LOCUS       DAZSIO010000001        70712 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZSIO010000001 DAZSIO010000000
VERSION     DAZSIO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.001"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..795
                     /locus_tag="ACHX5T_000001"
     CDS             <1..795
                     /locus_tag="ACHX5T_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR8465387.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="ACHX5T_000002"
     CDS             806..1897
                     /locus_tag="ACHX5T_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8465388.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="ACHX5T_000003"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="ACHX5T_000003"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR8465389.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..4901
                     /gene="lpoA"
                     /locus_tag="ACHX5T_000004"
     CDS             2865..4901
                     /gene="lpoA"
                     /locus_tag="ACHX5T_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR8465390.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4859..5254
                     /locus_tag="ACHX5T_000005"
     CDS             4859..5254
                     /locus_tag="ACHX5T_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR8465391.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5274..5864
                     /gene="diaA"
                     /locus_tag="ACHX5T_000006"
     CDS             5274..5864
                     /gene="diaA"
                     /locus_tag="ACHX5T_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR8465392.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5874..6449
                     /gene="dolP"
                     /locus_tag="ACHX5T_000007"
     CDS             5874..6449
                     /gene="dolP"
                     /locus_tag="ACHX5T_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR8465393.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6563..7603)
                     /locus_tag="ACHX5T_000008"
     CDS             complement(6563..7603)
                     /locus_tag="ACHX5T_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR8465394.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7676..8311)
                     /locus_tag="ACHX5T_000009"
     CDS             complement(7676..8311)
                     /locus_tag="ACHX5T_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR8465395.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8439..8957
                     /gene="yhbO"
                     /locus_tag="ACHX5T_000010"
     CDS             8439..8957
                     /gene="yhbO"
                     /locus_tag="ACHX5T_000010"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR8465396.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8937..9380)
                     /locus_tag="ACHX5T_000011"
     CDS             complement(8937..9380)
                     /locus_tag="ACHX5T_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR8465397.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9431..9733
                     /gene="yhbQ"
                     /locus_tag="ACHX5T_000012"
     CDS             9431..9733
                     /gene="yhbQ"
                     /locus_tag="ACHX5T_000012"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR8465398.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9720..10223)
                     /locus_tag="ACHX5T_000013"
     CDS             complement(9720..10223)
                     /locus_tag="ACHX5T_000013"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8465399.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10217..10741)
                     /locus_tag="ACHX5T_000014"
     CDS             complement(10217..10741)
                     /locus_tag="ACHX5T_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR8465400.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10950..11945
                     /locus_tag="ACHX5T_000015"
     CDS             10950..11945
                     /locus_tag="ACHX5T_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR8465401.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11954..12832
                     /locus_tag="ACHX5T_000016"
     CDS             11954..12832
                     /locus_tag="ACHX5T_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR8465402.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12913..13920
                     /locus_tag="ACHX5T_000017"
     CDS             12913..13920
                     /locus_tag="ACHX5T_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR8465403.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="ACHX5T_000018"
     CDS             complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="ACHX5T_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR8465404.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="ACHX5T_000019"
     CDS             complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="ACHX5T_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR8465405.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="ACHX5T_000020"
     CDS             complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="ACHX5T_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR8465406.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="ACHX5T_000021"
     CDS             complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="ACHX5T_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR8465407.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="ACHX5T_000022"
     CDS             complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="ACHX5T_000022"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR8465408.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="ACHX5T_000023"
     CDS             complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="ACHX5T_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR8465409.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21298..22242)
                     /gene="truB"
                     /locus_tag="ACHX5T_000024"
     CDS             complement(21298..22242)
                     /gene="truB"
                     /locus_tag="ACHX5T_000024"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR8465410.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="ACHX5T_000025"
     CDS             complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="ACHX5T_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR8465411.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22807..25455)
                     /gene="infB"
                     /locus_tag="ACHX5T_000026"
     CDS             complement(22807..25455)
                     /gene="infB"
                     /locus_tag="ACHX5T_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR8465412.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="ACHX5T_000027"
     CDS             complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="ACHX5T_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR8465413.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSAELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="ACHX5T_000028"
     CDS             complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="ACHX5T_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR8465414.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27654..27730)
                     /locus_tag="ACHX5T_000029"
     tRNA            complement(27654..27730)
                     /locus_tag="ACHX5T_000029"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27694..27696),aa:Met,seq:cat)
     gene            28078..29421
                     /gene="argG"
                     /locus_tag="ACHX5T_000030"
     CDS             28078..29421
                     /gene="argG"
                     /locus_tag="ACHX5T_000030"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR8465415.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29429..31055)
                     /locus_tag="ACHX5T_000031"
                     /pseudo
     CDS             complement(29429..31055)
                     /locus_tag="ACHX5T_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(31514..31600)
                     /locus_tag="ACHX5T_000032"
     tRNA            complement(31514..31600)
                     /locus_tag="ACHX5T_000032"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31564..31566),aa:Leu,seq:gag)
     gene            complement(31615..31947)
                     /gene="secG"
                     /locus_tag="ACHX5T_000033"
     CDS             complement(31615..31947)
                     /gene="secG"
                     /locus_tag="ACHX5T_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR8465416.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="ACHX5T_000034"
     CDS             complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="ACHX5T_000034"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR8465417.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33505..34353)
                     /gene="folP"
                     /locus_tag="ACHX5T_000035"
     CDS             complement(33505..34353)
                     /gene="folP"
                     /locus_tag="ACHX5T_000035"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR8465418.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="ACHX5T_000036"
     CDS             complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="ACHX5T_000036"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR8465419.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="ACHX5T_000037"
     CDS             complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="ACHX5T_000037"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR8465420.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37232..37525
                     /gene="yhbY"
                     /locus_tag="ACHX5T_000038"
     CDS             37232..37525
                     /gene="yhbY"
                     /locus_tag="ACHX5T_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR8465421.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37681..38157)
                     /gene="greA"
                     /locus_tag="ACHX5T_000039"
     CDS             complement(37681..38157)
                     /gene="greA"
                     /locus_tag="ACHX5T_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR8465422.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38405..39838
                     /gene="dacB"
                     /locus_tag="ACHX5T_000040"
     CDS             38405..39838
                     /gene="dacB"
                     /locus_tag="ACHX5T_000040"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR8465423.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            39902..40003
                     /locus_tag="ACHX5T_000041"
                     /pseudo
     CDS             39902..40003
                     /locus_tag="ACHX5T_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="ACHX5T_000042"
     CDS             complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="ACHX5T_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR8465424.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41212..42177)
                     /locus_tag="ACHX5T_000043"
     CDS             complement(41212..42177)
                     /locus_tag="ACHX5T_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR8465425.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="ACHX5T_000044"
     CDS             complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="ACHX5T_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR8465426.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="ACHX5T_000045"
     CDS             complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="ACHX5T_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR8465427.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43151..44122
                     /gene="ispB"
                     /locus_tag="ACHX5T_000046"
     CDS             43151..44122
                     /gene="ispB"
                     /locus_tag="ACHX5T_000046"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR8465428.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44349..44627
                     /gene="sfsB"
                     /locus_tag="ACHX5T_000047"
     CDS             44349..44627
                     /gene="sfsB"
                     /locus_tag="ACHX5T_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR8465429.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44675..45934)
                     /gene="murA"
                     /locus_tag="ACHX5T_000048"
     CDS             complement(44675..45934)
                     /gene="murA"
                     /locus_tag="ACHX5T_000048"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR8465430.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="ACHX5T_000049"
     CDS             complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="ACHX5T_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR8465431.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="ACHX5T_000050"
     CDS             complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="ACHX5T_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR8465432.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDMAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="ACHX5T_000051"
     CDS             complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="ACHX5T_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR8465433.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="ACHX5T_000052"
     CDS             complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="ACHX5T_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR8465434.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="ACHX5T_000053"
     CDS             complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="ACHX5T_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR8465435.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="ACHX5T_000054"
     CDS             complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="ACHX5T_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR8465436.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49715..50680
                     /locus_tag="ACHX5T_000055"
     CDS             49715..50680
                     /locus_tag="ACHX5T_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR8465437.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50694..51680
                     /gene="kdsD"
                     /locus_tag="ACHX5T_000056"
     CDS             50694..51680
                     /gene="kdsD"
                     /locus_tag="ACHX5T_000056"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR8465438.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51701..52267
                     /gene="kdsC"
                     /locus_tag="ACHX5T_000057"
     CDS             51701..52267
                     /gene="kdsC"
                     /locus_tag="ACHX5T_000057"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR8465439.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52264..52839
                     /gene="lptC"
                     /locus_tag="ACHX5T_000058"
     CDS             52264..52839
                     /gene="lptC"
                     /locus_tag="ACHX5T_000058"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR8465440.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52808..53365
                     /gene="lptA"
                     /locus_tag="ACHX5T_000059"
     CDS             52808..53365
                     /gene="lptA"
                     /locus_tag="ACHX5T_000059"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR8465441.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53372..54097
                     /gene="lptB"
                     /locus_tag="ACHX5T_000060"
     CDS             53372..54097
                     /gene="lptB"
                     /locus_tag="ACHX5T_000060"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR8465442.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54145..55578
                     /gene="rpoN"
                     /locus_tag="ACHX5T_000061"
     CDS             54145..55578
                     /gene="rpoN"
                     /locus_tag="ACHX5T_000061"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR8465443.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55601..55888
                     /gene="hpf"
                     /locus_tag="ACHX5T_000062"
     CDS             55601..55888
                     /gene="hpf"
                     /locus_tag="ACHX5T_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR8465444.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56006..56497
                     /gene="ptsN"
                     /locus_tag="ACHX5T_000063"
     CDS             56006..56497
                     /gene="ptsN"
                     /locus_tag="ACHX5T_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR8465445.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56543..57397
                     /gene="rapZ"
                     /locus_tag="ACHX5T_000064"
     CDS             56543..57397
                     /gene="rapZ"
                     /locus_tag="ACHX5T_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR8465446.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57394..57666
                     /gene="npr"
                     /locus_tag="ACHX5T_000065"
     CDS             57394..57666
                     /gene="npr"
                     /locus_tag="ACHX5T_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR8465447.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57879..58511
                     /gene="yrbL"
                     /locus_tag="ACHX5T_000066"
     CDS             57879..58511
                     /gene="yrbL"
                     /locus_tag="ACHX5T_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR8465448.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="ACHX5T_000067"
                     /pseudo
     CDS             complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="ACHX5T_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="ACHX5T_000068"
     CDS             complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="ACHX5T_000068"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR8465449.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="ACHX5T_000069"
     CDS             complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="ACHX5T_000069"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR8465450.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62547..63476)
                     /locus_tag="ACHX5T_000070"
     CDS             complement(62547..63476)
                     /locus_tag="ACHX5T_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR8465451.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64151..68611
                     /gene="gltB"
                     /locus_tag="ACHX5T_000071"
     CDS             64151..68611
                     /gene="gltB"
                     /locus_tag="ACHX5T_000071"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR8465452.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68624..70042
                     /gene="gltD"
                     /locus_tag="ACHX5T_000072"
     CDS             68624..70042
                     /gene="gltD"
                     /locus_tag="ACHX5T_000072"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR8465453.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70226..70630
                     /locus_tag="ACHX5T_000073"
                     /pseudo
     CDS             70226..70630
                     /locus_tag="ACHX5T_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
BASE COUNT        17316 a        19176 c        17558 g        16662 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccaccatta ccgtcatggt gcggtaaaag aagacgttgc gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata
     3721 tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac
     3781 aacctcagcc gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc
     3841 tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg
     3901 cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac
     3961 aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc
     4021 cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg
     4081 cactgaacca gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac
     4141 cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc
     4201 tggtgctgat cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt
     4261 ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac
     4321 gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag
     4381 cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg
     4441 accagttcac caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg
     4501 aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc
     4561 tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg
     4621 aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc
     4681 aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg
     4741 atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca
     4801 acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat
     4861 ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga
     4921 tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc
     4981 taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt
     5041 ttttgtcgag gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac
     5101 ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg
     5161 gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga
     5221 gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag
     5281 aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc
     5341 ttccggatgc catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca
     5401 aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca
     5461 tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata
     5521 atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg
     5581 tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc
     5641 gcgatattgt taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga
     5701 ccggctatga cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca
     5761 ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt
     5821 gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg
     5881 cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg
     5941 ccgtagtggg taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca
     6001 cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac
     6061 agattaagaa agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg
     6121 ggcagtcacc aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg
     6181 gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat
     6241 ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga
     6301 aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga
     6361 ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg
     6421 ttaccacagc gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg
     6481 ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc
     6541 atccgacatg ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag
     6601 acactgccac catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca
     6661 taatcagtga cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg
     6721 ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga
     6781 caaacaagca tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac
     6841 catcggcatg ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgt
     6901 aaaccgggat cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac
     6961 cgctctgtgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcggt gtttcacgca
     7021 cccatttttg caccagcgtc gcaatcagca acaccatcac cagcccggcc accagacgaa
     7081 tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca
     7141 ccggattgcc catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc
     7201 ccgccgcgac cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc
     7261 ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga
     7321 tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga
     7381 ggaaatagat catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa
     7441 ggatagattt accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt
     7501 gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc
     7561 actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata
     7621 agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt
     7681 cagctctttt gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca
     7741 tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg
     7801 gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc
     7861 ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt
     7921 cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag
     7981 cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta
     8041 ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc
     8101 cgagacaaca aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt
     8161 gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag
     8221 caatggcgtt aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc
     8281 ccgttgcgcc tgtaatcagt acctgactca tcttcgttcc tttacagaat tgtctgcctt
     8341 gcgctccccg gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg
     8401 aaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt
     8461 taatcactga tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag
     8521 ccggacacga agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag
     8581 gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg
     8641 ccctgttgct accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca
     8701 cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc
     8761 agctgctgat cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca
     8821 ttattgatgt taaaaatgcg ggcgtggaat tttacgatca ggaagtcgtg gttgataaag
     8881 atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac
     8941 gcctgctcgg tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg
     9001 tcggtgaatt tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca
     9061 accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg
     9121 cggatctcac ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt
     9181 accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc
     9241 gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag
     9301 agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg
     9361 atggcgatga gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc
     9421 gatgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta
     9481 taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc
     9541 gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg
     9601 ttcgctggca ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg
     9661 tctggtagcg gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat
     9721 taaaagcgat tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca
     9781 tccgctaaac gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg
     9841 tgatgcgccg ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc
     9901 actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc
     9961 agcccctgtc cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg
    10021 tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca
    10081 tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc
    10141 gcttccgcat cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg
    10201 ggaatttcta ctcgaattag catggttcac ctacagatgt ctgtttggtt tcaggcgcgg
    10261 ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg
    10321 gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat
    10381 cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg
    10441 caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc
    10501 tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat
    10561 gaatacttaa ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga
    10621 attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta
    10681 ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca
    10741 cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag
    10801 cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc
    10861 ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg
    10921 ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc
    10981 cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg
    11041 ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg
    11101 tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc
    11161 cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg
    11221 cgctgatcct cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg
    11281 aacgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc
    11341 gccattttga cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac
    11401 agctggcacg ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgtatta
    11461 tgtcggaagg ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg
    11521 gtgcgtgttc tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc
    11581 gcctgaacga agtgctgatc gaccgttatc aggacggcga aaacgcaggt tatccgacgc
    11641 tgtgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca
    11701 gtctcaatac cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa
    11761 ttgaaggccg ccagcgtagc ccggcgtatg tcagccaggc ggcgaaagtc tggcgtcagg
    11821 ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga
    11881 cgctcgggtc gatgtccgaa ggcacgcaaa ccacccttgg cgcgtatcac cgtaaatggc
    11941 agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac
    12001 gctggaagat ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga
    12061 agcggtatgc agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc
    12121 gctcgccgga agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc
    12181 tgaactgggc gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga
    12241 tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct
    12301 gaactgctac aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg
    12361 tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct
    12421 gggcattcgc aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta
    12481 ctccgcccgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg
    12541 ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt
    12601 actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc
    12661 catgcaggga ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat
    12721 gctcgacgcc ttccgcgcta atgaaaatgg cgctgtgcca ctgccgttga cggcaaacag
    12781 cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag
    12841 ctcactttgt taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca
    12901 aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata
    12961 cccgaaggct cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct
    13021 gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc
    13081 agtgctgcaa catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg
    13141 gggtctggcg gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc
    13201 acactcaata cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt
    13261 gaccaacgga caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc
    13321 cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc
    13381 ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt
    13441 gcacaactgg cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat
    13501 atgttgttcc aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa
    13561 aaaccatacg cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa
    13621 tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa
    13681 ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg
    13741 cagcaggcgc tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag
    13801 tcgatcctgc gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac
    13861 caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag
    13921 tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata
    13981 agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta
    14041 ctgatacacc ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc
    14101 aaacaccaga atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc
    14161 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    14221 cgctaaacca gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac
    14281 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    14341 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc
    14401 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
    14461 gcttaatgct tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc
    14521 cggacgaggg atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat
    14581 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
    14641 caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag
    14701 caaatacggc gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac
    14761 atgccccagc aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    14821 cgccgtcatg cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc
    14881 aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg
    14941 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat
    15001 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    15061 actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    15121 acagaaccag gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac
    15181 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    15241 aagcagcgac ggtgacgttt gggtggtggt tagtgttgcc atgagggctt ctctccagtg
    15301 aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac
    15361 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc
    15421 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
    15481 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    15541 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    15601 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt
    15661 catcggcttg ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc
    15721 tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct
    15781 gctgatgtcg ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc
    15841 gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg
    15901 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    15961 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    16021 ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc
    16081 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    16141 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    16201 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    16261 acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt
    16321 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    16381 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    16441 acgaccgtct ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    16501 cagcgcggcg ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt
    16561 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    16621 tgcttcgttt ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt
    16681 agtcacgctg gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    16741 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    16801 cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    16861 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    16921 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    16981 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    17041 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    17101 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    17161 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    17221 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    17281 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    17341 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    17401 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    17461 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    17521 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    17581 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    17641 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    17701 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    17761 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    17821 aagtgctgtt tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    17881 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    17941 tgcgctaact tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag
    18001 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    18061 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    18121 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    18181 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    18241 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    18301 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    18361 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    18421 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    18481 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    18541 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    18601 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    18661 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    18721 aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    18781 tctgcagtga tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct
    18841 gcaatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg
    18901 gcacggataa cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg
    18961 atggtatgga tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    19021 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    19081 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg
    19141 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    19201 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    19261 ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa
    19321 gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    19381 atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    19441 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    19501 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    19561 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    19621 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    19681 ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    19741 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    19801 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    19861 taagcatcgc tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    19921 accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg
    19981 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    20041 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca
    20101 accagatcca gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca
    20161 ttgatgtaac ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac
    20221 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa
    20281 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc
    20341 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg
    20401 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    20461 gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg
    20521 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    20581 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    20641 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    20701 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    20761 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    20821 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    20881 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    20941 aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    21001 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    21061 gctacctgaa cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    21121 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    21181 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    21241 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    21301 cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    21361 cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc
    21421 accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    21481 caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    21541 cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt
    21601 cacaatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc
    21661 gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga
    21721 gcagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac
    21781 ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt
    21841 tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa
    21901 agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat
    21961 ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    22021 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa
    22081 catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    22141 ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa
    22201 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    22261 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    22321 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    22381 cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    22441 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    22501 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct
    22561 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    22621 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    22681 tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc
    22741 gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca tggaacccta
    22801 aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga tcacatcgcc
    22861 agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc
    22921 gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt
    22981 gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc
    23041 tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat
    23101 ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag
    23161 gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc
    23221 agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt
    23281 ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc
    23341 agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt
    23401 caggacgatg ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag
    23461 tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc
    23521 aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac
    23581 gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc
    23641 cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc
    23701 cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag
    23761 gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac
    23821 ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga
    23881 cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag
    23941 ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac
    24001 caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc
    24061 gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat
    24121 tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc
    24181 aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt
    24241 tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac
    24301 aaccggcgcg cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc
    24361 cagctcgttt tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc
    24421 ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt
    24481 gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc
    24541 gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga
    24601 agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc
    24661 agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc
    24721 acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg
    24781 agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt
    24841 gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt
    24901 acgacgcgct tcttcttcag ctttacgctt gagctctgca gcttcctgct cacggcgggc
    24961 tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc
    25021 cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg
    25081 ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc
    25141 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    25201 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    25261 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    25321 caaagtctgt ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc
    25381 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    25441 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    25501 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat
    25561 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    25621 ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg
    25681 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    25741 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    25801 catcggcaca taggccagtt cttccagcgt cgagaaacct tcttctacca gaacagtcgc
    25861 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    25921 ctgatgctta gcctgcaggt cgtcaacggt cattacgttg agttcccaac cgctcagctg
    25981 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccagcttc
    26041 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    26101 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    26161 ctcgccgccc agttcagcag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc
    26221 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    26281 gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg
    26341 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    26401 ttccgggcga acggaataaa gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    26461 cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat
    26521 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    26581 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    26641 cgtctgggtg gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    26701 caagttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    26761 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    26821 gatctgtaca cggacgtcga tctcttgctc atattttttc tttgttgctg tcgccagcgc
    26881 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    26941 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    27001 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    27061 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    27121 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    27181 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    27241 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    27301 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg
    27361 aataaattcg atgcctacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    27421 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    27481 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    27541 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    27601 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    27661 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct
    27721 ccaccccgcg cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg
    27781 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    27841 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    27901 tgaatacttt tgccttcccc tgctctccct taagcgcatt attttacaaa aaacacacta
    27961 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    28021 ttcagtgaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    28081 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt
    28141 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    28201 actgcaaacc tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    28261 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    28321 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    28381 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    28441 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    28501 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    28561 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    28621 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    28681 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    28741 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    28801 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg
    28861 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    28921 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    28981 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    29041 gcgcatggtc gtcagttggg ccgtctgctg taccaggggc gttggtttga ctcccaggcg
    29101 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    29161 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    29221 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    29281 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    29341 tatgccaaaa ctggcctgct ttcctcctct gccacttcag gcctgccgca ggtggagaat
    29401 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt
    29461 gcttatcctc tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat
    29521 ccacaacccg tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt
    29581 ccggctttgt tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga
    29641 tattgttttc cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca
    29701 tcgggacatg atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct
    29761 tcgtagggtc acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt
    29821 ttaataattc aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc
    29881 aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag
    29941 ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct
    30001 gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt
    30061 ccatggcgcg catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg
    30121 agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt
    30181 aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg
    30241 cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt
    30301 caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag
    30361 actcccccac aatcaacacg taggtatcaa tacttgtatc cctgactgat aattgaaaat
    30421 acggcaccgt gtttgcaatt gagagtaatc tttgatgcgc tttcgctgct aaagcaaaat
    30481 agttgagatt gaaaaacggc gtataggtag caaatcacga cgctagtata tatggactga
    30541 acgctttttt attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg
    30601 aaatgacaat cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt
    30661 ttattattac tgccaaaaaa agaagggata aaaaagcaaa agcacataga taagggatat
    30721 acatccccag cattttcaca acttcatccg gatcactctg cagcacacta atcgcaaatc
    30781 catcattgaa agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg
    30841 cagtcagtaa cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt
    30901 taacaaccag gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gctccggaag
    30961 aggcgactaa gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt
    31021 taaaatcttc tagcaaattt gttgaatact gtcatccaga aatccaagtt cacaaccaat
    31081 ataaacaatt atcaacaatg ttcaatttta tatcagacat gtttttcaac tctgatccat
    31141 atcagaccga aacactgtta gttttttctt tttaaatgat gcgaccattg cgttctcagt
    31201 aattaaataa cattatcaat gcgtctctaa acattacgca gaacgtagcc atcagtagtt
    31261 atactgacag gagcaggatg gtttatcgag aagtaaacat atccgtattc gtaccggaac
    31321 tggagttgac acaataaagt gccaattatg tcagcagtag ggaaaagttt ataactaagg
    31381 cgtaccggca ccatcgtttc aaggtaccag ctacgagtaa agcaactgga cgagatacag
    31441 atacctgaca accattcatc agaccaggac caaaacgaaa agagacgctt ttcagcgtct
    31501 cttttctgga atttggtacc gaggacggga cttgaacccg taagccctat tgggcactac
    31561 cacctcaagg tagcgtgtct accaattcca ccacctcggc acggatacta ctttttagtt
    31621 cgggatatcg ctggtcggct tagccggagc agctggctga gtttgttcgg ttttcgccgg
    31681 tgcactcaga ttttcccatt cgctacctct attggttttg ttgctattga tgttacccag
    31741 caccagactg atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg tcatgaagtt
    31801 accagaacca cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg aggctcccat
    31861 atcagcgcct ttaccttgct gcagcatgat cagaccaaca aggccaattg ccacaataag
    31921 gaaaactact aaaagagctt cgtacataat caacctgttc cttgcggagt tgccgcgtac
    31981 caatgcttca accaataaag cgggagtttt taatctttcc cactgaagcg ggtgtgaata
    32041 ctaaccaaaa cgaatgacct tcgcaagggc aattttatcg cattgtatca actgcggaaa
    32101 aaaacagcaa aaccgatgtg ttcgccgaga aaaaaggcga caatcgccgc ctttttagcc
    32161 agttatctaa cgctttaaac ggcttttact gcatcggcga tgcggtgtgc aaattcagtc
    32221 acctgcgctt cgtcttcgcc ttccaccatc acgcgaatta acggttcggt gccggattta
    32281 cgcagcaaca cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt cacggcttta
    32341 actgactcat gctcaagcgg atcgccgcta cctgcggtgt aacgtacgtt aaccagaatc
    32401 tgcgggaaca ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt acgtgccatc
    32461 gccgccagca cctgcaagcc agcaacgatg ccgtcaccgg tagtggtttt atccagcagg
    32521 atcacatgac cggaattctc tgcaccgata cgccagcctt tctcctgcat tttttccagt
    32581 acgtagcggt cacccacttt cgcgcgcgca aatggaattc ctaactgttt cagcgccagt
    32641 tcaagcccca tgttgctcat caatgtaccc acagcaccac cacgcagctg gccctgacga
    32701 agaccttcac gcgcgatgat atacatgatc tgatcgccat cgactttatt gccttcatgg
    32761 tcaaccataa tcacgcgatc gccgtcgccg tcgaaggcaa taccgagatc cgctttttca
    32821 gccagcacac gagcctggag cgcgcgaacg tcggtagccc ccacttcggc attgatgttt
    32881 acaccgtttg gctcacaacc gatagcgata acgttcgccc ccagttcgcg cagcacgttc
    32941 ggcgcgatgt gataagtcgc accgtttgca caatccacca caatcttcag ttcactgagg
    33001 ctaagttcgt tcgggaacgt ggctttgcaa aactcgatat agcgacccgc ggcatcaacg
    33061 atacggctgg ctttacccag ttcagccgaa tcaacgcagc tgatctcctt ttccatttcc
    33121 gcttcgatgg cctcttctac cgcatccggc agtttagtgc cgtcgataga gaagaattta
    33181 atcccattat cgtagaacgg gttatgcgat gcagatatca caattccggc ctctgcgcgg
    33241 aaggtacgcg tcagataagc cacggccggt gttggcatcg ggccagtgaa gagtgcggaa
    33301 aggcccgctg ccgccagacc cgcttccagt gctgactcca gcatatagcc agaaatacgc
    33361 gtgtctttac caataataat cttacgggag ccgtggcgcg ccagcacttt acccgcggcc
    33421 caacccagct taagcacaaa atcaggtgtg atcggcgcat cccctacacg accacgaatc
    33481 ccatcggtac cgaaatattt acgattactc atagcgtttg ttttcctttg cagacagagt
    33541 ggcttccacc acccgcatcg cttctacggt ttctttgacg tcatgaacac gaatgatgtg
    33601 cgcgccttgc attgcggcaa tgaccgcaca ggccagacta ccgctcaggc gctcggacgg
    33661 ccccacgttc agcagctgcc caatcatcga ttttcgtgac atacccacca acagcggcag
    33721 gttgaaatgg tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg agagattttt
    33781 accgaaaccg aatccggggt cgagcaacaa tttctctttt gcgatacccg cctgctcgca
    33841 acgtgctatt tgctcaataa agtagcgatt cacttctgca aagacatcgt catacttcgg
    33901 agcttcctgc atggtttttg gatttccctg catatgcatc agacaaaccg gtaaaccggt
    33961 ttctgcagcc gcctccagag cgccaggttc ggaaagggag cggatatcat taataatgtg
    34021 agcgccaact ttcgctgact cacggatgac ttctggtttg gatgtatcga ccgagatcca
    34081 gacttcgaag cgttgagcaa ttgcctcaac cacaggaata acacgttgca actcttcttc
    34141 aacgctaact tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc
    34201 gccagcattg atcatcagat tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc
    34261 accatccgaa aaggaatcag gcgtgacgtt gaggatcccc attacgtgag gatggctaag
    34321 gtccagtgaa gtaccctggg caaagagttt cattatgtta tccctggtat gaattgataa
    34381 gaaaaaaccc cggagcacgc cccggggttt tcggtacaaa tacagtcatc tgatgcggga
    34441 acttacttgt cgcctaactg ctctgacatg gtgttacccg ggttcggcgt acgcggttca
    34501 tcaaccggac gaggagcctt tggactacca ttgtcgccag aattgttaga agcgcctggt
    34561 tcttcccagc ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt
    34621 gcgtcgatag tctcatattt catgagagca tctttcatcg catgcagaat atccatattg
    34681 tcggtcagaa gctgacgcgc acgattatag ttacgctcaa tcagtgcttt cacttcctgg
    34741 tcgatgatac gtgcagtttc atcggacata tgtttcgctt tcgctacgct acggccgagg
    34801 aatacttcac cttcttcttc cgcgtacagc agtggaccca atttctcaga gaagccccac
    34861 tgagtcacca tgttacgtgc caggttggtc gcaactttaa tatcgttgga cgcaccggta
    34921 gaaacatgtt ccggcccgta gatgatctct tctgccagac gaccaccgta cagcgtagaa
    34981 atctggcttt ccagtttctg acggctggcg ctgattgcgt cgccctcagg caagaagaaa
    35041 gtcacaccca gcgcacgacc gcgtgggata atcgtcactt tatgcaccgg atcgtgttcc
    35101 ggcaccaggc gaccgataat cgcatgaccc gcttcgtggt aagccgttga ttctttctgc
    35161 gcttccgtca tcaccatgga gcgacgttcc gcacccatca tgattttgtc tttcgctttc
    35221 tcgaactcaa ccatcgacac aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg
    35281 ttcaccaagt tcgccaggtc agcaccggag aaaccaggag taccacgagc aatgattgcc
    35341 gcgtcgatat cgggtgccaa tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga
    35401 ccgcgaacat ctggcaagcc gaccacaacc tggcggtcga aacggccagg acgcagcagg
    35461 gccgggtcga gaacgtccgg acggttagtc gcggcgataa cgatgatacc ttcgttacct
    35521 tcgaagccat ccatctcaac cagcatctgg ttcagagtct gttcacgttc atcgtgacca
    35581 ccgcccagac cagcgccacg ctggcggcct acggcgtcga tttcatcgat aaagatgatg
    35641 cacggtgccg ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc acccacaccg
    35701 acgaacattt ctacgaagtc agaaccggag atagtaaaga acggaacttt cgcttcgcct
    35761 gcaatcgctt tcgccagcaa cgttttaccg gtacccggag gaccgaccat caggacgcct
    35821 ttcgggatct taccgccgag tttctggaag cggctcggct cgcgcagata ctctaccagt
    35881 tcagcaactt cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc
    35941 tgatcttccg tcagcatgcg cgctttgctc ttaccaaacg acatggcacc tttgccaccg
    36001 ccgccctgca tttgacgcat gaagaagatc cagacaccaa tcagcaacag catcgggaac
    36061 caggagatga agatagaagc cagcaggctt ggttcttcag gcggttcacc gacaaccttg
    36121 acgttcttgg tcaacaggtt atccagtaat ttcggatcct gaaccggaat gtaagtggta
    36181 taacggttac tatctttctt ggtaacgttg atttcacgtc cgttgatacg cgcttcacga
    36241 acctggtcgt tattgacctc ttgtaggaag gtagagtaat ccaccttacg gccattagac
    36301 tcgctgggcc caaagctctg gaatactgac atcagcacaa cggcaatgac cagccagagt
    36361 attaggtttt tcgccatgtc actcaaggga ttaacctctt attacaactg tgttaaaaac
    36421 agcgtcagga tactctatat ccagcatctt tcaaactttc gtctgaaatc tcccggttag
    36481 ggtttacgcc cggtcgctac aatatacact tctcgcgaac gtgcacgaga agagtccggc
    36541 ttacgaactt tgaccttcgt aaacagggag cgaatttccc ttagatactc atcgaaacct
    36601 tcgccctgga acaccttcac tacaaaactg ccacctggcg ctaatacatc acgacacatt
    36661 tctagcgcca gttccaccag atacatggct cgggggatat ccaccgccgg tgttccgctc
    36721 atgtttggtg ccatatcgga catgacaacc tggactttgc tgtcgccaac gcgctccagc
    36781 agtgctttca tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac accaacgata
    36841 ggatccatag gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc
    36901 acatattgtg accaaccacc cggagcagca ccaaggtcga caaccgtcat tcccggttta
    36961 aagagtttgt cactttgctg tatttcatca agtttaaacc aggcacggga acgtaatccc
    37021 tttttctgtg cctgttgaac atatttatcg ctaaagtgtt cctgaagcca gcggctggag
    37081 ctggcagaac gcttcttacc tgtcatttaa ctttcccatg gggataactc atcgtaacca
    37141 attgcgtaaa tttttacgcg cctatttggt gatatatggg agatggcggt agaatgaccc
    37201 gttttcaatc ccaacgtaag caaaaaatac gatgaatctg agtactaaac aaaaacagca
    37261 cctgaaaggt ctggcacatc cgctcaagcc agttgttctg cttggcagta atggtttgac
    37321 cgaaggggtg ctggccgaga ttgaacaagc gttagagcac catgaactca tcaaggtgaa
    37381 aatcgccacc gaagatcgcg aaacaaaaac cttgatcgtg gaagctatcg tgcgcgaaac
    37441 cggcgcctgt aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc caactaaaga
    37501 acgtaaaatc tcgctgccac gctaagatta tcctaaagtt acacacattt gctgtgtaaa
    37561 acgaggggtt ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt gataaaaggc
    37621 cgcagagcgg cctttttcct ttctttacaa tacatcaaca tcttgagtat tgggtaattc
    37681 ttacaggtat tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac
    37741 cacaacatca tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat
    37801 caggttttgt ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc
    37861 agaatccaga ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat
    37921 tttggtgaca tcaatcacct gcgcgtttga cagcttggct tcgatgtctt tgatacggcc
    37981 ttcgcagaag ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc
    38041 atgctcacgc gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa
    38101 atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg
    38161 ttatacctct tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg
    38221 cccggcataa ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc
    38281 aggtcagcta caattcacat tttgatggtc attttaccct gaagttcccg aagggtcatc
    38341 gtttacttta tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga
    38401 gattatgcga ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca
    38461 ggccgcgaat gttgatgagt acgttactca actccccgca ggtgccaacc ttgccctgat
    38521 ggtgcaaaaa gtcggtgcgt cggcccccgc tattgattac cacagtcagc agatggcgct
    38581 gcccgccagt acccagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga
    38641 ttttcgtttc accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg
    38701 tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat
    38761 ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac
    38821 ctccattttc gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg
    38881 ctttagcgct ccgcctgctg ccgccatagt tgaccgcaac tgtttctccg tctcgctcta
    38941 cagtgcccca aagcctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac
    39001 gatgttcagc caggtacgca ccctcccccg tggttctgcc gaagcgcaat actgcgaact
    39061 ggatgtggtg ccaagcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc
    39121 tgagccgctc ccgttggctt ttgccgtgca ggatggagcc agctatgccg gtgcaattct
    39181 gaaagatgag ttaaaacagg cgggtatcac ctggagcgga acactgctgc gccagactca
    39241 ggttaacgaa cctggaacgg tagttgccag taaacagtcg gccccgctgc acgatctgct
    39301 taagattatg ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatcgg
    39361 ccatgcgcgc ttcaatgtgc ctggaacatg gcgggctggg tcggacgccg tgcgtcagat
    39421 cctgcgccag caagccggtg tcgatattgg taacaccatt attgccgatg gttcagggct
    39481 ttcgcggcat aacctgattg cccccgccac catgatgcag gtgctgcaat acattgccca
    39541 acacgacaat gaacttaact ttatctccat gctgccgctt gcgggctatg acggctcttt
    39601 gcagtaccgt gcaggtctgc atcaggcggg cgtggatggc aaagtctcag cgaaaaccgg
    39661 ttcgttgcag ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat
    39721 ggcatttgta caatatcttt ctggctatgc agtaaaaccc gcggatcagc gtaatcgccg
    39781 tattccgtta gtgcgttttg aaagccgttt gtacaaagat atttatcaga acaattagtc
    39841 aaaaagaaac cccggcgcat ggccggggtt tcagattatt gacaaagtgc gctttgttta
    39901 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat cgtgcaaatt caatatattg
    39961 caatcatctt gtaggcctga taagtgtagc gcatcaggct tatttggcat tggtcattag
    40021 tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcgtcatc
    40081 ccagtcttca tcatcctctt cagcaatctc ttcaagctgc tgacggtgat aatcatccca
    40141 catgaattcg actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat
    40201 gataaaggtc atcacatccc agcagagatc tttcacgccc agtccgctcg ccgcagagat
    40261 cagataatat ttatcttccc agcccagcgc ttcagcgatc gctttcgctt tctcttcggc
    40321 ttctgcctta tccagcagat cgatcttgtt gaagactaac caacgcggtt tcgccgccag
    40381 atcctggctg tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt
    40441 gccgtcaatc ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt
    40501 caggaagcga atgcccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc
    40561 ggcaacaacg aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt
    40621 ggtaaacgga taatccgcca ctttcggttt agccgccgat accgcacgaa taaaggtcga
    40681 tttacccgcg tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag
    40741 cagctcacgc ttatcgcccg gcgtaccgtt agttttctgc cgtggtgtac ggttaactga
    40801 cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg
    40861 accgtgttta gtcatatcac ccatggtttc accagtaccc tggtcgatta cacgcgtacc
    40921 taccggcact ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc
    40981 gttctgaccg cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag
    41041 gttctcgtcg gcttccatcc atacgtcacc accatcaccg ccgtcgccgc catccgggcc
    41101 gcctttcgga atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc
    41161 aacgaccaga atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc
    41221 tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc
    41281 aatggcggaa tacatcgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt
    41341 taacatcggt ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag
    41401 cgtaaacagt ggggtgagcg tgatgatcgc gctcacctgc gctgcctgcc agcgagccat
    41461 cgcttccgcc aaggcgccat atcctaccaa ggtattcagt ccgcaaaaaa ttaaacatgc
    41521 gagctgccag tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcgat
    41581 tgtacataaa gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac
    41641 cttttgcgcc acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc
    41701 ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac
    41761 cagaccgctc aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag
    41821 gataaatacg ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc
    41881 ggtcggactc aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc
    41941 ggtcgccact gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa
    42001 cctcttcttc accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa
    42061 cacgattgtc ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc
    42121 ccagcaaatt gctgtggtaa gcgccaaaag aatgccaatg cctgcctgct gcttcatacc
    42181 gtattccctg caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta
    42241 tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa
    42301 aacttattca gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt
    42361 cactttaccg tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc
    42421 agcgtggaat ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc
    42481 accgaaacgc ttaacgccca agcgtttagc ttctgaatcg cgaccgttac gtgtggagcc
    42541 gccagccttt ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta
    42601 attttcacat cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga
    42661 cgaaacttaa cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt
    42721 acgccgccat caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc
    42781 acttcagcga actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc
    42841 tgaccttcgc ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac
    42901 tccgcttccg cgcacacctt ttcaatgatt cagagtgcgc tataaatatt cacaataggg
    42961 cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa
    43021 aaacacaact tgtacggtaa cgtttatctg tgccattttt tcagtacaat taccctatat
    43081 tcctaaccat aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc
    43141 ggcttttgcg atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt
    43201 taatgcggca atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta
    43261 ttacatcgtc agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg
    43321 agctgttggc tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca
    43381 cacggcgact ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc
    43441 taccgccaac gccgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac
    43501 ccgcgcgttc cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga
    43561 agctgtaaac gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga
    43621 catcactgaa gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc
    43681 tgccgcgcag tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca
    43741 ggattatggg cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa
    43801 tgccgatggc gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac
    43861 gctgccgctg ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac
    43921 cgccatcgaa caaggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc
    43981 ttgtggatct cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc
    44041 agcgttacag gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat
    44101 cgctgttcaa cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgc gggatgattc
    44161 cagtaaattc cataaaaaca ctaattcagc gctataacac ctgaaaaaag ggcgatgaat
    44221 ctttatatat tctgaatatt cacactcttt acaggaactt tttagcgcaa tagaccatca
    44281 ggagtatagt gatgcccgac agaagaagtg ttctgaatga aagcgactaa cttaaggagt
    44341 gaggaaagat ggaaagtaat ttcattgact ggcatcccgc tgacatcatt gcaggtttgc
    44401 gcaagaaagg aacgtcaatg gcggcggaat ctcgcagaaa tggtttgagt tcctcaacgc
    44461 tggcaaacgc attatcgcgc ccatggccga aaggagagat gattattgcg aaagccctgg
    44521 gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc catgagttta
    44581 tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt
    44641 agccccgcga acggggctgc cagctctcag acgattactc gcctttcaca cgctcaatat
    44701 ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa
    44761 tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg cttgctgatg
    44821 cacgcagatc ggttgccata acctgtgcac cagaaagttt ttcaacacca tgacaaataa
    44881 cggtattgct ttcgatttcg gcgtgcgcgc ccatacggct cagctctggc acatgcataa
    44941 agcggttttc aaagaccgtt tcggtgatga acccggtccc ttcagccacc aggttcaaca
    45001 gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta cgtacgttaa
    45061 cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg acttcgatgt
    45121 ccgctccagc atcacgcagt ttcgccagca cggcgtccag agtatctggc tgcgcgttac
    45181 ggcagataat tttgccacga gagatcgccg ccgccaccag gaaagtaccg gtttcgatac
    45241 gatccggcag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg atgacgatac
    45301 gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc gcggtatcga
    45361 cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt gccttccgcc agggttgcag
    45421 cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata tgtgcgcctt
    45481 tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg gtcgcgccta
    45541 attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta caaccgccag
    45601 gtagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagcgcc cagatagaag
    45661 cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa
    45721 tatgcacaga accattacgt tctactttcg cacccagctg gcttagcagt ttcattgatg
    45781 tatcgacgtc tttcagtttc gggacgttct ggatctctac cggttcttcc gccagtagtg
    45841 cggcaaaaag aataggcaga gcagcatttt tagcgccgga aattgtgact tcgccctgga
    45901 gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt taacaattca
    45961 tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc
    46021 actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc gcaatatatt
    46081 ccatcagcgg gccatagacc gtctgctgtt ttttaacccg actcataccg tcaaacaact
    46141 cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact tcctggaggg
    46201 agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct tcaatcatca
    46261 gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata agcaaaaagc
    46321 ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca ggcggttaac
    46381 gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa ttatacaatt
    46441 tcgcgagggt atacactttg tcgtttaccc cctgaagcgt cacgttgttg ccctgctttt
    46501 tcgccatatc gataagatgg agcagcagtg ccagtccccc cgtatccacg cgggacacac
    46561 ggctaagatc gatgcaggta atccccttca ccgcctcctc acgcatttcc caaagcggta
    46621 gcagaacgtc ctgatccagc tctccggata acgccagcgt gtcacccgtc tgcatccagc
    46681 tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag aaatcgattt
    46741 cagttgcgca gtcaggccgt cgataccttt ggtacgcagc agcgttcccc actcgttttg
    46801 tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcttgcc agtttcccgt
    46861 ctgggagttt ttacgccact ggaagtccag acgcaccggc ggacggccat tcgggtcaat
    46921 aatggtaacg cgaataggca caatggtttt atcgcccagc ggctgttctg gcgcaatctg
    46981 ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa gcctgcttca ggtactcacg
    47041 gaaagcggca aagtaggctt cacgttgagc aggggtcgca ctcttgtaat actggcccag
    47101 caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat caacaatggt
    47161 acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc gatcgaacgt
    47221 tttctgcgcc gcctcgtcca tcagcttata aggattggtc tggtctgccg cggttgccgc
    47281 actcagaggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt
    47341 ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac ctggcgcagc
    47401 agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa ggaactgacc
    47461 aatgagatct tccagcacca tcgcagactt ggtgtcctga attgtatcgc catccttcag
    47521 gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat attgttctcc
    47581 cagcaggccg gaagtacgaa tgctcagcga actggtatca ggaatgtggt tataacgttg
    47641 ttcaatttcc agcgttacgc gcggcagata ggttttcggg tccagcgtaa tatccgccac
    47701 ccggcccaca acaacaccac caatgctgac cggagagcgc gctttcaggc cgccaatgtt
    47761 atcgaacgtc gcataaagcg tgtaggtcga ttcagtacgt atggacgtca cgttcgccgc
    47821 cttcaggcaa acaaacagcg ccgccagcag tgctgctaat aaaaagatac ccacccaaat
    47881 ttcatttttt ttcgtttgca tgaactcaat tcccaaacat caatgcggtc agcacaaaat
    47941 ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc cggctaatcc
    48001 cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc accgtgatgg
    48061 cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg acggcatttt
    48121 gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg accagcgatc
    48181 cgccccagat ccccacggcg acgaagataa ccgtcaacag tggtaatgaa ataaccccag
    48241 cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc tccatactgg
    48301 agagttgctc tgtagcgcgc atcaggccga tttctgcggt taacgccgaa ccagcacgcc
    48361 cggcaaacaa cagcgcggca accaccggcc ccagttcacg cagtagcgat aacgccacca
    48421 gcatacccag actggtttcc gcgctataag tggtcagaac cagataacct tgcagcccca
    48481 acaccattcc gatgaacacg ccagaaacta caataatcag catcgacagg acgccgacat
    48541 tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg ccgaccagcg
    48601 cattgaataa cattaacccg gcccgcccga acgttctcag ggttttaatc cctttatgtc
    48661 cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa aagatcagcg
    48721 tgataatcgc cggcaggata gcggaacgga acaggcccgt cagctatccc gtccagaaac
    48781 tgacgtacgc gcggatcagg attcgtctgc aacgcctggg cactgccatg agcgacaatt
    48841 tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg cacatcgtga
    48901 gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag cttcaccagt
    48961 acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat gagatccggc
    49021 tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga aagttcagaa
    49081 ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat caccgtacta
    49141 tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac gttgtcaaat
    49201 acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg tttacgcact
    49261 gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag gatctcacca
    49321 tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc gatgcccgat
    49381 ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt atcgaagatg
    49441 cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc cacagactgc
    49501 tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa tttaaccttg
    49561 aacccaacat atttacagaa tattacccgc cgtggttagc gaaagctggc atttgtttta
    49621 ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa aatcttcagc
    49681 ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg cactgttaat
    49741 tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg cgtctattct
    49801 ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca gtattggtac
    49861 atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac gcgatttagc
    49921 cgttggtaca gccctcggct caaacattat caatatattg ctgatcctcg gtctggctgc
    49981 gctggttcgt ccttttaccg tccattccga tgttctgcgc cgtgaattac ccttaatgtt
    50041 gttggtcagc gtggtggccg gttccgtact ctatgacgga caacttagtc gaagcgatgg
    50101 tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac ttgcacgtca
    50161 ggggactgac agcctgacca gagagcagct tgcagagctg ccgcgtgacg gcggattgcc
    50221 cgtcgcgttt ttatggctcg gcattgcgct tatcatcatg ccagtggcca cgcggatggt
    50281 ggttgataac gccacggtgc tggcgaatta ctttgccatc agcgagttga cgatggctct
    50341 gacggcaatt gctatcggaa ccagtctgcc ggaactggca accgcaatag cgggggttcg
    50401 caaaggtgaa aacgacattg ctgtcggaaa tatcattggc gcaaacattt ttaatattgt
    50461 catcgtgttg ggtttacccg cgctgataac gccaggagag attgatccac tggcgtacag
    50521 tcgtgactac agcgtgatgt tgctggtgag cattattttt gcgttgctgt gctggcggcg
    50581 ctccccgcaa ccgggccgtg gtgtaggggt attattaact ggcggattta tcgtatggct
    50641 ggcgatgttg tactggttat cgccaatact cgttgaataa ctggaaacgc attatgtcgc
    50701 acgtagagtt acaaccgggt tttgactttc agcaagcagg taaagaagtc ctggcgattg
    50761 aacgtgaatg cctggcggag cttgatcaat acatcaatca gaatttcacg cttgcctgtg
    50821 aaaagatgtt ctggtgtaaa gggaaagttg tcgtcatggg gatggggaaa tcggggcaca
    50881 tcgggcgcaa aatggccgca acgtttgcca gcaccggtac accttcattt ttcgtccatc
    50941 ctagtgaagc cgcgcatggt gatttaggca tggtcacccc acaggatgtg gtgattgcta
    51001 tctctaactc aggtgaatcc agcgaaatca cggccttaat tccagtgctt aagcgtcttc
    51061 acgtaccgtt aatctgcatc accggtcgcc cggagagcag catggcgcgc gccgcagatg
    51121 tgcatctgtg tgttaaagta gcgaaagaag cctgtccgtt agggctggca ccgaccagca
    51181 gcaccaccgc cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg
    51241 gctttactgc tgaagatttt gcgctctcac acccaggcgg cgcactgggt cgtaaacttc
    51301 tgctgcgcgt aaacgatatt atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg
    51361 ccagtctgcg tgacgcattg ctggaagtta cccgcaaaaa tcttggtatg actgtcattt
    51421 gcgatgacaa tatgatgatt gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg
    51481 atatgggcgt ggatgttcgt cagttaagta ttgccgatgt gatgacgccg gggggaatac
    51541 gtgtgcgccc tggcattctg gccgttgagg cactgaactt aatgcagtcc cgccatatca
    51601 cctccgtgat ggttgccgat ggcgaccatt tactcggtgt gttacatatg catgatttac
    51661 tgcgtgcagg cgtagtgtaa agattcaagg ataaacaaca atgagcaaag caggtgcgtc
    51721 gcttgcgacc tgttacggcc ctgtcagcgc cgacgttatg gcaaaagcag agaacattcg
    51781 tctgctgatc ctcgacgttg atggcgtact gtcagatggc ctgatttata tgggcaataa
    51841 cggcgaagag ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac
    51901 ctctgatatt gaagtcgcta tcattaccgg gcgaaaggct aaactggtag aagatcgttg
    51961 tgccacattg gggatcactc acttgtatca ggggcagtca aacaaactga tcgcctttag
    52021 cgatttgctg gaaaaattgg cgattgcccc ggaaaatgtg gcttatgttg gcgatgatct
    52081 catcgactgg ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc
    52141 actgttgatc ccgcgcgccg attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg
    52201 cgaagtttgc gacttattac tcctggcgca gggcaaactg gatgaagcca aagggcaatc
    52261 gatatgagta aagccagacg ttgggttatc attgtgctat cactggcggt tctggtgatg
    52321 atcggcatta atatggccga aaaagacgat accgcccagg tggtcgtcaa caacaatgat
    52381 cccacctata aaagcgagca tacggacacg ctcgtctata acccagaagg ggcactaagc
    52441 tatcgattga ttgctcaaca cgttgaatat tattccgatc aggccgtttc gtggtttacg
    52501 cagccggtac ttaccacgtt tgataaggat aaaatcccga catggtccgt aaaagcagat
    52561 aaagccaagc tgaccaatga ccggatgctc tatttatatg gacacgttga agtcaacgca
    52621 cttgtgccag actctcaact tcgcagaatc acgacggata acgcgcagat caatctggtg
    52681 acgcaggatg ttacctctga agacctcgtc acgttatacg gaacaacatt taactccagc
    52741 ggtctgaaaa tgcgcggcaa cttacgcagc aagaacgccg agctgattga aaaggttaga
    52801 acatcctatg aaattcaaaa caaacaaact cagccttaat cttgtgcttg ccagctcact
    52861 tctggccgcc agcattccgg catttgccgt aaccggagac actgatcagc cgatccacat
    52921 tgaatcggac cagcaatctc ttgatatgca aggcaacgtg gttaccttta ccggtaatgt
    52981 catcgtcacc cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg
    53041 cggcgaacaa ggtaaagaag tgattgacgg atacggtaaa ccggcaacgt tctaccagat
    53101 gcaggacaac ggtaaacccg ttgaaggtca cgcttcccag atgcactacg aactggcaaa
    53161 agattttgtc gttctgacgg gtaatgctta tctgcagcag gtcgatagca acattaaggg
    53221 cgataagatc acttacctgg tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa
    53281 gcgcgtaacc accgttctgg tgccgtcgca gctgcaggac aaaaacaaca aaggccagac
    53341 cccggcacag aagaagggta attaattcgt tatggcaaca ttaactgcaa agaaccttgc
    53401 aaaagcctat aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca actccgggga
    53461 aattgtcggt ctgctggggc caaacggtgc cggtaagacc accactttct acatggttgt
    53521 aggcattgtg ccgcgcgatg cgggcaacat cattattgat gatgacgata tcagtctgct
    53581 gcctctgcat gcacgcgcgc gccgcggtat cggctatctg ccacaggaag cctccatttt
    53641 ccgtcgcctc agcgtttacg ataacctgat ggcggtactg caaattcgtg acgacttgtc
    53701 tgctgaacaa cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca ttgagcacct
    53761 gcgtgacagc atggggcagt cactctccgg gggtgaacgt cgccgtgtag aaattgcccg
    53821 cgcactggct gcgaatccga aatttattct gctcgacgaa ccgtttgccg gggttgaccc
    53881 gatctcggtt atcgacatta aacgcatcat tgaacacctg cgcgacagcg gcctgggcgt
    53941 gctgatcact gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt
    54001 cagtcagggg catttgatcg cccacggcac gcctacagaa atcttacaag acgaacacgt
    54061 taagcgtgta taccttgggg aagacttcag actctgatag ggtagaagtt tgcgacgttt
    54121 tagcaggaga gtacgattct gaacatgaag caaggtttgc aactcaggct tagccaacaa
    54181 ctggcgatga cgccacagct ccaacaggca attcgtctgt tgcagttgtc gacgctggaa
    54241 cttcagcagg agctacagca ggcgctggag agtaatccgc tgcttgagca aatcgacact
    54301 catgaagaaa tcgacacccg cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg
    54361 ctcgaacaaa aagagatgcc ggaagagctg ccgctcgatg ccagttggga caccatttac
    54421 accgctggta caccatccgg caccagcggt gactacattg acgacgagct gccggtctac
    54481 cagggcgaaa cgacgcagac cttgcaggat tacctgatgt ggcaggtcga gctgacaccg
    54541 ttttccgaca ctgaccgcgc tattgctacc tctatcgtcg atgccgttga tgaaaccggt
    54601 tatctgactg tcccgctgga agatattctc gaaagtatgg gcgatgaaga gatcgacatc
    54661 gacgaggttg aagccgtcct taagcggatc caacggtttg acccggtcgg tgtagcggca
    54721 aaagatctgc gtgactgcct gctaatccaa ctctcccaat tcgataagac cacgccgtgg
    54781 ctggaagagg ccagattgat cattagcgat catctcgatc tgttagccaa tcacgacttc
    54841 cgcactttaa tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg
    54901 atccagtcgc tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc
    54961 attccagatg tgctggtgcg taagcataac ggtcactgga cggtagaact caacagtgac
    55021 agcattccgc gtctgcaaat caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac
    55081 gatggtgaca gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt
    55141 ctggaaagcc gtaacgatac gctactgcgc gtgagtcgct gtatcgttga acagcagcaa
    55201 gccttctttg agcaaggtga agaatatatg aaaccgatgg tactggccga tatcgcccag
    55261 gctgtcgaaa tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata cctgcatagt
    55321 ccacgaggca tttttgaact gaagtatttc ttttccagtc acgtcaatac cgagggcggc
    55381 ggcgaagctt cctccacggc gattcgtgca ctggtgaaga aattaatcgc ggcggaaaac
    55441 ccagcgaaac cgttgagcga cagcaagtta acctctttgc tgtcggaaca aggtatcatg
    55501 gtggcacgcc gcactgttgc gaagtaccga gagtctttat ccattccgcc gtcaaaccag
    55561 cgtaaacagc tcgtttgacc caaccgataa ggaagacact atgcagctca acattaccgg
    55621 aaataacgtc gagatcaccg aggcactgcg cgaatttgtt acagctaaat ttgccaaact
    55681 tgagcaatat tttgaccgaa tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac
    55741 ccacacctca gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg
    55801 tcaggacatg tacgctgcca ttgatggctt aattgataag ctggcacgcc agttgaccaa
    55861 acataaagat aaactgaaac aacactaatt gtccgggcaa ttagcatgtg catggcggcc
    55921 tgttgtgcgg cacaacgggc catttgtacg gttaatgctc cgagcctgtt ccactgtttg
    55981 agtgggcagg ttcttaggtg aaattatgac aaataatgat acaactctac agcttagcag
    56041 tgttcttaac agggaatgta cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct
    56101 ggaaatcatc agcgagctgg cggcgaaaca acttagcctg ccgcctcagg tggtttttga
    56161 agctatcctg acgcgggaaa aaatgggcag taccggtatc ggcaatggta ttgccattcc
    56221 gcatggcaaa cttgaagaag atactctgcg tgccgttggc gttttcgttc agctcgaaac
    56281 gcctatagct ttcgatgcca tcgacaacca accggtggac ttactttttg ccctgctggt
    56341 gccggcagac caaactaaaa cgcacctgca tactctgtcg ctggtggcga aacgtctggc
    56401 ggacaaaacc atctgccgcc gtttacgcgc agcccagagc gatgaagagc tgtatcaaat
    56461 cattacggat accgaaggta ctccggatga agcgtagtta ttcggtaatg tctcttttag
    56521 acgttgtgag gagaaacagt acatggtact gatgatcgtc agcggacgtt caggttcagg
    56581 taaatctgtc gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg ataaccttcc
    56641 cgtagtgttg ttacccgatc tggctcgaac tctggccgat cgcgagattt ctgccgccgt
    56701 cagcattgat gttcgtaata tgccggagtc accagaaata ttcgaacagg cgatgagtaa
    56761 cctgcctgac gctttctcac cgcaactact gttcctggat gccgaccgta ataccttaat
    56821 tcgtcgttac agtgacacgc gccgactgca tccgctttcc agcaaaaacc tgtcgctgga
    56881 aagtgctatc gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag cggatctgat
    56941 tgtcgatacc tcagaaatgt ccgttcacga gctggctgaa atgctgcgta cccgtctgct
    57001 gggtaaacgt gaacgcgaac tgaccatggt ctttgagtct ttcggcttca aacacggtat
    57061 ccctatcgat gcagattacg tctttgacgt gcgcttcttg ccgaacccgc actgggatcc
    57121 gaaactgcgt ccaatgacag gtcttgataa acctgtcgcc gcgttcctcg accgccacac
    57181 agaagtacac aattttatct accagacgcg aagctatctt gagctatggt tacctatgct
    57241 ggaaaccaac aaccgtagct acctgacggt cgccattggt tgtaccggcg ggaagcaccg
    57301 ttcggtgtat attgcagagc aactggcaga ctacttccgc tcgcgcggta aaaacgtcca
    57361 gtcacgccat cgtacgctgg aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc
    57421 acaaacaagc tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt
    57481 tttgacgctg aagtgctgtt acgtaatgac gaaggcaccg aggctgaagc caacagcgtt
    57541 attgcgctgc tgatgttgga ttctgccaaa ggacggcaga ttgaagttga agcgaccggt
    57601 ccacaggaag aggaagcact ggccgccgtt atcgccctct ttaattctgg ttttgatgaa
    57661 gattaatctt catcactttg acatacaact cccttcaagc tcccccgaca ataagaaaat
    57721 cacgtactga aatcgttctc aatcaacgtc atttgtacat tttgtgcgct tttcactttc
    57781 agaagaacct taagaaaacc ttaagaggca ttgtttaggt tttgtttaag ttaatcgacc
    57841 atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa
    57901 gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct
    57961 gtatcaagat tgtctaccat cgtggcgatg gtggcgataa agagatccgc cgtgagttaa
    58021 agtactacgc gcatcttggt cgccggttaa aagactggag tggaataccg cgctatcacg
    58081 gtaccgttga gacggattgc ggaaccggtt atgtctacga tgtgattgcc gattttgacg
    58141 gcaagccttc cattacgctg accgaatttg ctgaacaatg ccgttacgaa gaagacattg
    58201 cacaactgcg ccagctactg aaacagctga agcgttattt gcaggataac cgtatcgtga
    58261 cgatgtcgct gaagccgcag aatatcctct gtcaccgcat cagtgagtca gaggttatcc
    58321 cggtcgtctg cgacaacatt ggtgaaagca cattgattcc actggcgacc tggtcgaagt
    58381 ggtgttgtct gcgtaagcaa gagcggttat ggaaacgatt tatcgcccaa ccggcgctgg
    58441 ctattgccct gcagaaagat ctgcaacctc gtgagagcaa aacgctggcc ctcacttcgc
    58501 gcgaagctta atccagctgg tgttgttgca taaacggctc accgcctaac tgatacatct
    58561 gccgtaaaat ccacgcctga cggctacgca cgtagcccga tggcgaggag actttgaaac
    58621 gaagtggatt aggtaataca gctgccagta atgcagcttc cgaccgggta agtttgctcg
    58681 cgggtttgtg gaaataacgt tgtgccgcag cttcgacgcc aaacacaccg tcgccaaatt
    58741 cggcgatatt caggtaaacc gtcaggatac gctttttgct ccagactgtt tctatcccca
    58801 gcgttaatcc agcttccagc ccttttcgta cccagctacg cccatcccat aaaaagagat
    58861 tttggccgtc tgctgagaaa tcgttgaagc accgcgaata cggttttcat tgcgctcgtt
    58921 gtgcgccagg gctttctcaa tggaagcgac atcaaagccc cagtgctcag gaaatttctg
    58981 atcttctgcg gcaatcaccg ccagtcccat ccacggcgag atttgatcca tactgaccca
    59041 gtcagaatgt gccacgtaac gaaaattgcc atgcagccag gcgctgacct gtcgctcgac
    59101 cattaccgct gagaagggaa caggcgcaac gctaaacaac gcgatgcccc cgccccagaa
    59161 aacggcgagg acaaccatta accgcaaaag aaaacggcga acgaaactaa acaccgttaa
    59221 gcggctttta ctcattcagc cagaaccagc acgcgggaaa ccagcttatc aatgccgctc
    59281 gccgcttctg caatgttctg cgccagcata tatgctgggg tggtgacaat cttattgtct
    59341 tcatcaacca cgatatcatc gacaggacac ggcacatgct ccgcgcccat ctcttccagc
    59401 acttctgcgg tatcgatatc agtaccgatg gtcaaacgca gcgggaaatc gaaaattttc
    59461 ggcagcatcg ccggggcaat acacataaaa ccaagcggtt ttccggcctg atgcatcgct
    59521 tgtgccagcg cctttaattc acggtcaacg gtgcattcgc taccaagact ggcaaaattg
    59581 cttaaattct tcgccgcgcc aaaccccccc ggcacaatca acgcatccag ttcagcggca
    59641 tcggcctggg ccagaggacg gatttcaccg cgcgttattc gtgccgcttc aatcagcaca
    59701 ttgcgcgttt ccgtcatcgc ttcgccagtt aaatggttga taacatcaac ctgctgctta
    59761 tccggtgcaa agcagaccgc ctgcgcaccg ctgcgtgaaa tagctaacaa cgtcaacacc
    59821 gcttcatgaa tttcagaacc gtcatagacg ccgcatccgc tcagaattac gccaattttc
    59881 ttcattgtga tcatcctttc gcaattgact gaaacacatt aattttttta ataaaaatgg
    59941 tacgcatcac acatttaact gattcatgta acaaatcatt taagttttgc tatcttaact
    60001 gcgtgcggcc tgaaaaacag tgctgtgccc ttgtaactca tcataataat ttacggcgca
    60061 gccaagattt ccctggtgtt ggcgcagtat tcgcgcaccc cggtctagcc ggggtcattt
    60121 tttagtggct tttgccaccc acgctttcag cacttctacg tcgtgacgcc actcttcttt
    60181 catctcttca atccattcac cgacgttatc ttcccaggcg ggaaggtcag gagactgaat
    60241 ttgctgaccc agctgttgca gatggcgtaa ccccactgac cccgccgcac ctttaatttt
    60301 atgtccttcc tcaacaatgc cttttttatc ctgcgccgtc agattcgact ccagcacgct
    60361 gacatagccc ggcatcatct tctcaaacac cgctaacccg tcggtgatca gcttcggtcc
    60421 tacaagttcg agatactgtt ccagcatggg aatatcgagc aatgcttctg atttactgtt
    60481 ctcttctgtc gtcaccgtac tctcctcatc atcctgggta tcccagaatt tcttgatcat
    60541 cgcggttagc gccggaaccg aaagcggctt actcagcaca tcatccattc cagcattgag
    60601 gtactcttgt ttgtctttca gcacgttagc ggttaaggcc accagcggcg gtaaatcctc
    60661 gcgcggataa cgcttcgtca gttcacgaga gatatccagc ccggtcatat ctggcaactg
    60721 aatatccagc aacaccaggt cgtattcgcc cggtttaaac atctccagcg ccgccttgcc
    60781 ggtcatggcg acatcaacgc tgttacctaa tttttccagc acagaacgcg cgacaatcac
    60841 gttcagttca atgtcttcca ccagcagcac attcagcgcc ggtaaaggca tatcgtcttc
    60901 atcaaacgca tcatcgacct cttctgccac cgacggtgcg tggatcgtca acgtaaaggt
    60961 tgaacctttg ccctgttcac tggtaaccgt aatatcgccg cccatatttt tcgccagacg
    61021 acgagaaacg gccagaccaa taccggtgcc ggtggcaggt ttaccgccat gactgtcttt
    61081 cacctggtaa tacatggcga agattttatc cagctcatcc tgcggaatgc cgataccaga
    61141 atcttccact tcaaaatgca gcatatcgcc ttcatcgtag cgcacgcgca cggtaacctg
    61201 gccttgctgg gtgaatttga cggcgttact gatgaggttc cacaggatct gccgtaaacg
    61261 cgtcccgtcg gtaatgacct gatgcggtaa tggcagcgta ggctccaggt taaagcgcaa
    61321 tcctttttgt tgcgcctgca atgcggagag attttccaga tcggcaagga agctggtgaa
    61381 atcaaccggc tgattatcaa gctggacctt gcgccgttcc atcttatcca tgtcgataat
    61441 atcgttaaag atattcccca gcgtgacggc cgaaacatgg atagttttga gatatttttc
    61501 ctgctcggcg gtgagttcgg tatccagcag aatgcggctc aggccgacga taccgttcag
    61561 cggtgtacgc aattcgtgac tgatggtgga aataaacgtc gttttgtcgc ggctggcgcg
    61621 ttcaagcgca tcctgatacc gcttacgctc ggtaatgtcg cgaccaaagc ccatcaaacc
    61681 gtgacgttta cccacgcggt cgtagtacgg cactttacgg atttcaaagc aggctttgcg
    61741 cccgtccggg taatccagcc actgttcata ggtcagtgac acattatgac ggaacacttt
    61801 ttcatcggtt tcaatgactt ttgcggcggc ttccggtgag taaacatcag caggtttcag
    61861 gtgaaccagt tgtttttcgc tttttccggt cagcagctcc atcgcgcggt tacagccgga
    61921 aaactcttta tcttcgttac gataaaaaac caagtcgggt gaagcatcaa ggaaggaacg
    61981 taagaatgag gattgctgct cgagctgaat ttgtgtctct tcgcgctctt tgatttcaat
    62041 tttcagttgg ccgaaggttt cctgcagttc tgcttccgct ttttcacgaa cggcaatttc
    62101 ctgatttagc tgggcaatat tatcttttaa ctgaacgttg aggctcaaat cgcgctcgcg
    62161 catctcctcc agtttttgca ccagccgtga cagacgttgt cgtgactcct ccagttgctc
    62221 gacgaccacc gatagaaagt agaccgccca cggcgtaatc agcaaaccaa agaagataga
    62281 acgaataaca tcaatgcttt cgacctgacc atgcagcacc atggttaccg ccatttgtac
    62341 cacaatggca agaacgacga gggccagcgc cagcaacatt gagaagcgca ccagacctaa
    62401 cttcatcatc aggtcaacat aatactgcgc cagcagacga atttgcttca ttagggaatt
    62461 ccttcacgac aacctgcccc aataataccc aattctgaca gttacgttat aaattgtgtg
    62521 agaaatgcgg aattcccctc aacgacctac tccgttggag gtagccacgg acgtcccagc
    62581 gccgatccct gtacgccatg ttcattcaga tagcgatcca gctcgaccat tcccgtccat
    62641 cgattttcgc accacagcgg agcaagcagc gtcgggcgac gggcgctggc ggaaatgcgg
    62701 tggtagatca cttccggtgg cgtatggcga atcatttctc cggcagtgag cgtataatcc
    62761 tccagttcaa taccgttcaa acgccccgct tcccaggctt tcgccataat gctgcctttc
    62821 acaatatgca gcggatgcag ctttatgccg tccacgctgg tttcaaccac tcgctccagc
    62881 gtttgcaaac attcagcctg cccttcgcca ggcaggccga caattaagtg ggaacacacc
    62941 ttcagcccgc gctgacgtgc cagctgggtt gtacgctgat aacaggcaaa atcatggccg
    63001 cggttgatgc gatgcagtgt tttgtcgtgg gcggtttgta gccccagctc cagccacact
    63061 tcgtaaccct ggtccttata ttcgcaaagc agatccagca ccgcatccgg cacgcagtcc
    63121 gggcgggtac caacacacaa accgacaata ttggcctggc tcaccgcctg ctgatacata
    63181 gaacgcagca cctgaacttc cgcaaaggtg ctggtatacg cctgaaagta ggccagatag
    63241 cgtttagcgc ggttaactaa attcgcctgg tgcgccagtt gctcggcaat ggaacgatgc
    63301 tgctgcgctt catcggcaaa cgaggcaaca ttacagaatg tgcagccacc acgcccgatg
    63361 gtaccgtcac ggttagggca gctaaaaccg ccgtgcagcg tcagcttatg caccttttgc
    63421 ccataacgac gggtgagatc accaccaaac atattgacta atttctgtaa ctgcataatc
    63481 tgatagaccg cgccttgaaa agaggccaaa gcctgccatt tttagctcaa ttcggcgatg
    63541 acctggatca atcgtcccgg cctgctttta tcaactgcat aatcaatcaa aattaccgaa
    63601 atttcatgca caatcacata aatcactttt gcttatcttg tgtcagattt ttttatctcc
    63661 ttatggattt taggcaaaaa cagtagcatg aaacgtcatt accaattaag gcagtataaa
    63721 atgctggttt tctcgtcagt tcaaggcagg ataagggtta acacgccttt atgacagtca
    63781 ggaattgact gtttctctaa cgactctcct tttagcctta aagataaaat ctattttaat
    63841 ttcagctatt taataaagaa ttttgcgcta aagcacattt ctgtaccaat aagcttgcca
    63901 tttgacctgt atcagctttc ccgataagtt ggaaatccgc tggaagcttt ctggatgagc
    63961 agcctgctca tcatatttat gcagtaattg agatcccctc ttcaccgtat taaccgatgc
    64021 gaaaaggaca acaagggggc gaatgcgagg cgcgcgtatg acacgcaaac cccgtcgcca
    64081 cgctctttct gtgcccgtgc gcagcggttc ggaagtgggg ttcccgcaga gcctggggga
    64141 ggttcacgat atgttgtacg ataaatccct tgagagggat aactgtggtt tcggcctgat
    64201 cgcccacata gaaggcgaac ctagccacaa ggtagtgcgt actgcaatac acgcactggc
    64261 ccgcatgcag caccgtggcg cgattctcgc cgatggtaaa accggcgacg gttgcggctt
    64321 gctgttacaa aaaccggatc gcttttttcg catcgttgcg caggagcgcg gctggcgttt
    64381 agcaaaaaac tacgctgtcg ggatgctctt cctgaataaa gatcctgaac tcgccgctgc
    64441 cgcacgccgc atcgttgaag aagaactgca acgcgaaacc ttgtcgattg tgggctggcg
    64501 tgatgtcccc actaacgaag gcgtgctggg tgaaatcgcc ctctcctctc tgccacgcat
    64561 tgagcaaatt tttgtgaacg ccccggcagg ctggcgtcca cgcgatatgg agcgccgtct
    64621 gtttatcgcc cgccgccgca ttgaaaagcg tctcgaagcc gacaaagact tctacgtctg
    64681 tagcctgtcg aatctggtga acatctataa aggtctgtgt atgccggcgg atctgccgcg
    64741 cttttatctg gatcttgcgg acctgcgtct ggaatcggcc atttgcctgt tccaccagcg
    64801 cttctccact aacaccgtac cgcgctggcc gctggcgcaa ccgttccgct atctggcgca
    64861 taacggtgaa atcaacacca tcaccggtaa ccgccaatgg gcgcgtgcgc gtacctataa
    64921 attccagacg ccgctgatcc ctgacctgca cgacgccgcg ccattcgtca acgaaaccgg
    64981 ttctgactcc agttccatgg ataacatgct ggaactgctg ctggcaggcg ggatggatat
    65041 catccgcgcc atgcgcctgt tagtgccacc cgcctggcag aacaacccgg atatggaccc
    65101 ggaactgcgt gccttctttg actttaactc catgcatatg gagccgtggg atggtccggc
    65161 gggcatcgtg atgtccgatg gtcgcttcgc tgcctgtaac ctcgaccgta acggtctgcg
    65221 tccggcacgc tacgtcatta ccaaagacaa gctcatcacc tgcgcctctg aagtcggtat
    65281 ctgggattac cagcctgacg aagtggtcga aaaaggccgc gtcgggccag gcgaactgat
    65341 ggtgatcgac actcgcagtg ggcgtattct gcactcggca gaaaccgatg acgatctgaa
    65401 aagccgccac ccttataaag agtggatgga gaaaaacgtc cgccgactgg taccgtttga
    65461 agatctgccc gatgaagaag tgggtagccg cgaactggac gacgacacgc ttgccagcta
    65521 ccagaaacag tttaactaca gcgcggaaga gctggactcc gtaattcgcg tactgggcga
    65581 aaacggtcag gaagcggtcg gttcgatggg cgatgatacc ccattcgccg tgctctccag
    65641 tcagccgcgc attatttacg actacttccg ccagcagttt gcccaggtga ctaacccgcc
    65701 aatcgacccg ctgcgtgaag cgcatgttat gtcgctcgcc accagtatcg gtcgtgaaat
    65761 gaacgtcttt tgcgaagcag agggccaggc gcaccgttta agctttaaat cgccgattct
    65821 gctctactcc gatttcaaac agctcacgac gatgaaagag gagcactacc gcgcagatac
    65881 gctggatatc acctttgacg tcactaaaac cacgctcgaa gcgacagtca aagagctatg
    65941 cgacaaagcc gaaaaaatgg tacgtagcgg caccgtgttg ctggtgctct ccgaccggaa
    66001 tatcgctaaa gatcgcctgc cggttccggc tccgatggcg gttggcgcga tccagacccg
    66061 tctggtcgat caaagcctgc gttgcgatgc caacatcatc gtcgaaaccg ccagcgcccg
    66121 cgatccgcac cacttcgccg tgttgctggg tttcggcgcg acggctattt atccgtacct
    66181 tgcctatgaa acgctgggcc gcctggtaga cacccacgcg attgccaaag attatcgtac
    66241 cgtgatgctc aactaccgta acggcatcaa caaaggcttg tacaaaatca tgtccaaaat
    66301 gggcatctcc accatcgcct cttaccgctg ctcgaaactg tttgaagcgg tcggtctaca
    66361 cgatgatgta gtgggcctgt gcttccaggg cgcggtcagc cgcattggtg gggcaagctt
    66421 tgaagacttc cagcaggatc tgctgaatct gtcgaaacgt gcctggctgg cgcgcaagcc
    66481 gattagccag ggcggcctgc tgaaatacgt ccacggcggc gaataccacg cctacaaccc
    66541 ggacgtggtg cgcacgctgc aacaggcggt acaaagcggc gagtacagcg actatcagga
    66601 atacgcgaag ctggttaatg agcgtccggc aaccacgctg cgcgatctgc tggcaattac
    66661 gccgggtgaa aacgcggtca acattgctga tgttgaaccg gcaagcgaac tgtttaaacg
    66721 ctttgatacc gccgcgatgt ctatcggcgc gttaagcccg gaagcccacg aggcgctggc
    66781 ggaagcgatg aatagcatcg gcggtaactc gaactccggt gaaggcggcg aagacccggc
    66841 gcgctatggc actaacaaag tgtcgcgcat caagcaggtg gcttctggtc gcttcggcgt
    66901 tactccggcg tatctggtca atgccgacgt cattcagatt aaagtcgccc agggcgcgaa
    66961 gccgggcgaa ggtggtcagt tgccgggcga caaagtcact ccttacatcg ccaaactgcg
    67021 ctattcggtg cccggagtga cgctgatctc cccgccgccg caccacgata tctactctat
    67081 cgaagactta gcgcagctta ttttcgacct caagcaggtt aacccgaaag cgatgatctc
    67141 cgttaagctg gtgtccgaac caggcgttgg caccatcgcg actggcgtgg cgaaagctta
    67201 tgcggacttg atcaccatcg cgggctatga cggcggcacc ggcgcaagcc cgctgtcgtc
    67261 agtgaaatat gcaggctgcc cgtgggaact cggtctggtg gaaacccaac aggcgctggt
    67321 tgccaacggc ttgcgtcata aaattcgttt gcaggttgat ggcggcctga aaacgggtgt
    67381 cgatatcatc aaagcggcga ttctcggcgc agaaagcttc ggcttcggca ctggcccaat
    67441 ggtggcgctc ggctgtaaat atctgcgtat ttgccacctg aacaactgcg ccacgggtgt
    67501 agcaactcag gatgacaaac tgcgtaagaa tcactatcac ggcctgccgt tcaaggtgac
    67561 taattacttt gagtttatcg cccgtgaaac ccgcgagctg atggcacagc tgggagtaac
    67621 gcgtctggtg gatctgattg gtcgcaccga cctgctgaaa gagctggacg gtttcaccgc
    67681 caaacagcag aaactggcgc tgtcgaagct gctggagact gccgagccac atgcaggtaa
    67741 gtcactctac tgcactgaaa acaacccgcc gtttgataac ggcctactga acgcgcagtt
    67801 gctgcaacag gcgaaaccgt ttgtcgatga acgccagagc aaaaccttct ggttcgatat
    67861 ccgcaacacc gaccgttctg tcggcgcgtc gctttcaggc tatatcgccc agacgcacgg
    67921 cgatcagggg ctggcagccg atcctatcaa agcgtacttc aacggcaccg caggccagag
    67981 tttcggtgtg tggaacgcgg gcggcgtgga actgtacctg accggggatg ccaacgacta
    68041 tgtcggtaaa ggcatggcgg gcggcttaat cgccattcgt cctccggttg gttccgcctt
    68101 ccgcagccat gaagcaagca ttatcggcaa cacctgcctg tatggcgcga ccggtggtcg
    68161 tctgtatgcc gcaggccgcg cgggtgaacg tttcggcgtg cggaactccg gtgctatcac
    68221 cgtggtagaa ggcattggcg acaacggctg tgaatatatg acgggcggta ttgtctgtat
    68281 tctgggtaaa accggcgtta acttcggcgc gggcatgacc ggcggcttcg cttacgttct
    68341 cgatgaaagc ggtgatttcc gcaaacgcgt taacccggaa ctggtcgaag tcttaagcgt
    68401 cgacgatctg gcgatccatg aagagcatct gcgcggtctt atcaccgagc atgttcagca
    68461 taccggttct cagcgcggtg aagagattct ggcgaactgg tcaaccttcg ccacgaaatt
    68521 cgcgctggtt aaaccgaagt ccagtgatgt aaaagcactg ctgggtcacc gtagtcgtag
    68581 cgcagcagag ttgcgcgtgc aggcgcagta aggggtagca acaatgagtc agaatgttta
    68641 tcaatttatc gacctgcagc gcgttgatcc gccaaagaaa ccgctgaaga tccgtaaaat
    68701 tgagtttgtt gaaatttacg agccgttttc cgaaggccag gccaaagcgc aggctgaccg
    68761 ctgtctgtcg tgcggcaacc catactgcga gtggaaatgc ccggtacaca actacatccc
    68821 aaactggctg aagctcgcca acgaggggcg tatttttgaa gcggcagaac tgtcgcatca
    68881 gaccaacaca ctgccggaag tatgcggacg tgtctgcccg caagaccgtc tgtgcgaagg
    68941 ttcctgcacc ctgaacgatg agttcggtgc ggtgaccatc ggcaacattg agcgttatat
    69001 caatgataaa gcgttcgaaa tgggctggcg tccggatatg tccggcgtga agcaaaccgg
    69061 taaaaaagtg gcgattatcg gtgcaggccc ggcaggtctg gcgtgtgcgg atgtcctgac
    69121 gcgtaacggc gtaaaagccg ttgtcttcga ccgtcatcca gaaattggcg ggctgctgac
    69181 cttcggtatt ccggccttca agctggaaaa agaggtaatg acgcgtcgcc gtgaaatctt
    69241 caccggcatg ggtattgaat tcaaactcaa taccgaagtg ggccgcgacg tacagctgga
    69301 cgatctgctg agtgattacg atgccgtgtt ccttggcgtt gggacttatc agtcaatgcg
    69361 cggcgggctg gaaaacgaag acgccgatgg cgtgtacgca gcgctgccgt tccttatcgc
    69421 caacaccaaa cagttaatgg gctttggtga aacccgcgac gaaccgttcg tcagcatgga
    69481 aggcaaacgc gtggtagtac ttggcggtgg cgacactgcg atggactgcg tgcgcacgtc
    69541 tgtacgccag ggggcaaagc acgttacctg tgcctatcgt cgtgatgaag agaacatgcc
    69601 gggttcccgc cgcgaagtga aaaacgcgcg ggaagaaggc gtagagttca aattcaacgt
    69661 ccagccactg gggattgaag tgaacggtaa cggcaaagtc agcggcgtaa aaatggtacg
    69721 taccgaaatg ggcgaaccgg atgccaaagg ccgtcgccgc gcggagatcg ttgcaggttc
    69781 cgaacatatc gttccggcag atgcggtgat catggcgttt ggtttccgtc cacacaacat
    69841 ggaatggctg gcaaaacaca gcgtcgagct ggattcacaa ggccgcatca tcgctccgga
    69901 aggcaacgac aacgctttcc agaccagcaa cccgaaaatc tttgctggcg gcgatatcgt
    69961 ccgtggttcc gatctggtcg ttaccgccat cgccgaaggt cgcaaagcgg cagacggcat
    70021 tatgaactgg ctggaagttt aagctctgtc ccagcgccgc ctcctcagaa aagagtcggc
    70081 gctgaaattc tcctttcttt ctggaaatcg cctcgcataa tctgaagaaa accaatgtaa
    70141 tacgctcatc tggattgtta ccggtagatg cccggcgtat agctgattcc attattcaac
    70201 gcagcgccat ataaagggac agataatgga atcgctctca gagagaacct caacaggcta
    70261 ccagcaaatc cacaacggca ttattcatct ggtcgatagc gcccgaacgg aaacggtacg
    70321 tagcgttaac gcgctgatga ccgcgacgta ctgggaaatt ggccgacgga ttgtcgaatt
    70381 tgaacaaggt ggtgaggcca gagcagctta tggtgcacag ttgattgatc ggctatcgaa
    70441 ggatttaagt cgacggtata aacgtggggt ctctgcaaaa aacttacgcc aaatgaggct
    70501 tttttacctc ttttttcaac atgttgaaat tcgccagaca gtgtctggcg aattaacacc
    70561 attgggaatt ccgcagacac catctgcaga attcccatcg gcaaagattt ggcagacgct
    70621 gtctgccaaa ttgagaggct gtttgatatt tccacagctt ttgtattgca tctcctattt
    70681 tcggtttgta tattcatgca aataaacgaa gg
//
LOCUS       DAZSIO010000002        65883 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZSIO010000002 DAZSIO010000000
VERSION     DAZSIO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65883
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.002"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..458)
                     /gene="folK"
                     /locus_tag="ACHX5T_000074"
     CDS             complement(<1..458)
                     /gene="folK"
                     /locus_tag="ACHX5T_000074"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR8465454.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRA"
     gene            complement(455..1852)
                     /gene="pcnB"
                     /locus_tag="ACHX5T_000075"
     CDS             complement(455..1852)
                     /gene="pcnB"
                     /locus_tag="ACHX5T_000075"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR8465455.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTT"
     gene            complement(1912..2808)
                     /gene="gluQRS"
                     /locus_tag="ACHX5T_000076"
     CDS             complement(1912..2808)
                     /gene="gluQRS"
                     /locus_tag="ACHX5T_000076"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR8465456.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWMLTAVPESAIVNSTFSNASC"
     gene            complement(2875..3330)
                     /gene="dksA"
                     /locus_tag="ACHX5T_000077"
     CDS             complement(2875..3330)
                     /gene="dksA"
                     /locus_tag="ACHX5T_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR8465457.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3508..4212)
                     /gene="sfsA"
                     /locus_tag="ACHX5T_000078"
     CDS             complement(3508..4212)
                     /gene="sfsA"
                     /locus_tag="ACHX5T_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR8465458.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4227..4757)
                     /gene="thpR"
                     /locus_tag="ACHX5T_000079"
     CDS             complement(4227..4757)
                     /gene="thpR"
                     /locus_tag="ACHX5T_000079"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR8465459.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4831..7260
                     /gene="hrpB"
                     /locus_tag="ACHX5T_000080"
     CDS             4831..7260
                     /gene="hrpB"
                     /locus_tag="ACHX5T_000080"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR8465460.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPKILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7456..9990
                     /gene="mrcB"
                     /locus_tag="ACHX5T_000081"
     CDS             7456..9990
                     /gene="mrcB"
                     /locus_tag="ACHX5T_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR8465461.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10210..12453
                     /gene="fhuA"
                     /locus_tag="ACHX5T_000082"
     CDS             10210..12453
                     /gene="fhuA"
                     /locus_tag="ACHX5T_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR8465462.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12504..13301
                     /gene="fhuC"
                     /locus_tag="ACHX5T_000083"
     CDS             12504..13301
                     /gene="fhuC"
                     /locus_tag="ACHX5T_000083"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR8465463.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13301..14191
                     /gene="fhuD"
                     /locus_tag="ACHX5T_000084"
     CDS             13301..14191
                     /gene="fhuD"
                     /locus_tag="ACHX5T_000084"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR8465464.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14188..16170
                     /gene="fhuB"
                     /locus_tag="ACHX5T_000085"
     CDS             14188..16170
                     /gene="fhuB"
                     /locus_tag="ACHX5T_000085"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR8465465.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16205..17485)
                     /gene="hemL"
                     /locus_tag="ACHX5T_000086"
     CDS             complement(16205..17485)
                     /gene="hemL"
                     /locus_tag="ACHX5T_000086"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR8465466.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17651..17698
                     /gene="yadX"
                     /locus_tag="ACHX5T_000087"
     CDS             17651..17698
                     /gene="yadX"
                     /locus_tag="ACHX5T_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR8465467.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17710..19131
                     /gene="clcA"
                     /locus_tag="ACHX5T_000088"
     CDS             17710..19131
                     /gene="clcA"
                     /locus_tag="ACHX5T_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR8465468.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19155..19220
                     /gene="yadW"
                     /locus_tag="ACHX5T_000089"
     CDS             19155..19220
                     /gene="yadW"
                     /locus_tag="ACHX5T_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR8465469.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19213..19557
                     /gene="erpA"
                     /locus_tag="ACHX5T_000090"
     CDS             19213..19557
                     /gene="erpA"
                     /locus_tag="ACHX5T_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR8465470.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19604..20227)
                     /locus_tag="ACHX5T_000091"
     CDS             complement(19604..20227)
                     /locus_tag="ACHX5T_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR8465471.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20265..21065)
                     /gene="btuF"
                     /locus_tag="ACHX5T_000092"
     CDS             complement(20265..21065)
                     /gene="btuF"
                     /locus_tag="ACHX5T_000092"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR8465472.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21058..21756)
                     /gene="mtnN"
                     /locus_tag="ACHX5T_000093"
     CDS             complement(21058..21756)
                     /gene="mtnN"
                     /locus_tag="ACHX5T_000093"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR8465473.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21840..23357
                     /gene="dgt"
                     /locus_tag="ACHX5T_000094"
     CDS             21840..23357
                     /gene="dgt"
                     /locus_tag="ACHX5T_000094"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR8465474.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23487..24911
                     /gene="degP"
                     /locus_tag="ACHX5T_000095"
     CDS             23487..24911
                     /gene="degP"
                     /locus_tag="ACHX5T_000095"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR8465475.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25066..26222
                     /gene="cdaR"
                     /locus_tag="ACHX5T_000096"
                     /pseudo
     CDS             25066..26222
                     /gene="cdaR"
                     /locus_tag="ACHX5T_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26311..26697)
                     /locus_tag="ACHX5T_000097"
     CDS             complement(26311..26697)
                     /locus_tag="ACHX5T_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR8465476.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27011..27835)
                     /gene="dapD"
                     /locus_tag="ACHX5T_000098"
     CDS             complement(27011..27835)
                     /gene="dapD"
                     /locus_tag="ACHX5T_000098"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR8465477.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27866..30538)
                     /gene="glnD"
                     /locus_tag="ACHX5T_000099"
     CDS             complement(27866..30538)
                     /gene="glnD"
                     /locus_tag="ACHX5T_000099"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR8465478.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30600..31394)
                     /gene="map"
                     /locus_tag="ACHX5T_000100"
     CDS             complement(30600..31394)
                     /gene="map"
                     /locus_tag="ACHX5T_000100"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR8465479.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31762..32487
                     /gene="rpsB"
                     /locus_tag="ACHX5T_000101"
     CDS             31762..32487
                     /gene="rpsB"
                     /locus_tag="ACHX5T_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR8465480.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32745..33596
                     /gene="tsf"
                     /locus_tag="ACHX5T_000102"
     CDS             32745..33596
                     /gene="tsf"
                     /locus_tag="ACHX5T_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR8465481.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33743..34468
                     /gene="pyrH"
                     /locus_tag="ACHX5T_000103"
     CDS             33743..34468
                     /gene="pyrH"
                     /locus_tag="ACHX5T_000103"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR8465482.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34760..35317
                     /gene="frr"
                     /locus_tag="ACHX5T_000104"
     CDS             34760..35317
                     /gene="frr"
                     /locus_tag="ACHX5T_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR8465483.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35409..36605
                     /gene="ispC"
                     /locus_tag="ACHX5T_000105"
     CDS             35409..36605
                     /gene="ispC"
                     /locus_tag="ACHX5T_000105"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR8465484.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36794..37552
                     /gene="ispU"
                     /locus_tag="ACHX5T_000106"
                     /gene_synonym="uppS"
     CDS             36794..37552
                     /gene="ispU"
                     /locus_tag="ACHX5T_000106"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR8465485.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37565..38422
                     /gene="cdsA"
                     /locus_tag="ACHX5T_000107"
     CDS             37565..38422
                     /gene="cdsA"
                     /locus_tag="ACHX5T_000107"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR8465486.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38434..39786
                     /gene="rseP"
                     /locus_tag="ACHX5T_000108"
     CDS             38434..39786
                     /gene="rseP"
                     /locus_tag="ACHX5T_000108"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR8465487.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39816..42248
                     /gene="bamA"
                     /locus_tag="ACHX5T_000109"
     CDS             39816..42248
                     /gene="bamA"
                     /locus_tag="ACHX5T_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR8465488.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42370..42855
                     /gene="skp"
                     /locus_tag="ACHX5T_000110"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42370..42855
                     /gene="skp"
                     /locus_tag="ACHX5T_000110"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR8465489.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42859..43884
                     /gene="lpxD"
                     /locus_tag="ACHX5T_000111"
     CDS             42859..43884
                     /gene="lpxD"
                     /locus_tag="ACHX5T_000111"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR8465490.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43989..44444
                     /gene="fabZ"
                     /locus_tag="ACHX5T_000112"
     CDS             43989..44444
                     /gene="fabZ"
                     /locus_tag="ACHX5T_000112"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR8465491.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44448..45236
                     /gene="lpxA"
                     /locus_tag="ACHX5T_000113"
     CDS             44448..45236
                     /gene="lpxA"
                     /locus_tag="ACHX5T_000113"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR8465492.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45236..46384
                     /gene="lpxB"
                     /locus_tag="ACHX5T_000114"
     CDS             45236..46384
                     /gene="lpxB"
                     /locus_tag="ACHX5T_000114"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR8465493.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46381..46977
                     /gene="rnhB"
                     /locus_tag="ACHX5T_000115"
     CDS             46381..46977
                     /gene="rnhB"
                     /locus_tag="ACHX5T_000115"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR8465494.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47014..50496
                     /gene="dnaE"
                     /locus_tag="ACHX5T_000116"
     CDS             47014..50496
                     /gene="dnaE"
                     /locus_tag="ACHX5T_000116"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR8465495.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50509..51468
                     /gene="accA"
                     /locus_tag="ACHX5T_000117"
     CDS             50509..51468
                     /gene="accA"
                     /locus_tag="ACHX5T_000117"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR8465496.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51567..53708
                     /gene="ldcC"
                     /locus_tag="ACHX5T_000118"
     CDS             51567..53708
                     /gene="ldcC"
                     /locus_tag="ACHX5T_000118"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR8465497.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53765..54154
                     /locus_tag="ACHX5T_000119"
     CDS             53765..54154
                     /locus_tag="ACHX5T_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR8465498.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54219..55517
                     /gene="tilS"
                     /locus_tag="ACHX5T_000120"
     CDS             54219..55517
                     /gene="tilS"
                     /locus_tag="ACHX5T_000120"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR8465499.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55566..55826)
                     /gene="rof"
                     /locus_tag="ACHX5T_000121"
     CDS             complement(55566..55826)
                     /gene="rof"
                     /locus_tag="ACHX5T_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR8465500.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55813..56013)
                     /locus_tag="ACHX5T_000122"
     CDS             complement(55813..56013)
                     /locus_tag="ACHX5T_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR8465501.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56179..56724
                     /locus_tag="ACHX5T_000123"
     CDS             56179..56724
                     /locus_tag="ACHX5T_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR8465502.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56721..57143
                     /gene="arfB"
                     /locus_tag="ACHX5T_000124"
     CDS             56721..57143
                     /gene="arfB"
                     /locus_tag="ACHX5T_000124"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFR8465503.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57157..57867
                     /gene="nlpE"
                     /locus_tag="ACHX5T_000125"
                     /gene_synonym="cutF"
     CDS             57157..57867
                     /gene="nlpE"
                     /locus_tag="ACHX5T_000125"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR8465504.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58022..58846)
                     /locus_tag="ACHX5T_000126"
     CDS             complement(58022..58846)
                     /locus_tag="ACHX5T_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR8465505.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(58899..60617)
                     /gene="proS"
                     /locus_tag="ACHX5T_000127"
     CDS             complement(58899..60617)
                     /gene="proS"
                     /locus_tag="ACHX5T_000127"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR8465506.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60728..61435)
                     /gene="tsaA"
                     /locus_tag="ACHX5T_000128"
     CDS             complement(60728..61435)
                     /gene="tsaA"
                     /locus_tag="ACHX5T_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR8465507.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61432..61836)
                     /gene="rcsF"
                     /locus_tag="ACHX5T_000129"
     CDS             complement(61432..61836)
                     /gene="rcsF"
                     /locus_tag="ACHX5T_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR8465508.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61954..62769)
                     /gene="metQ"
                     /locus_tag="ACHX5T_000130"
     CDS             complement(61954..62769)
                     /gene="metQ"
                     /locus_tag="ACHX5T_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR8465509.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62809..63462)
                     /locus_tag="ACHX5T_000131"
     CDS             complement(62809..63462)
                     /locus_tag="ACHX5T_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR8465510.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63455..64486)
                     /gene="metN"
                     /locus_tag="ACHX5T_000132"
     CDS             complement(63455..64486)
                     /gene="metN"
                     /locus_tag="ACHX5T_000132"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR8465511.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64674..65249
                     /gene="gmhB"
                     /locus_tag="ACHX5T_000133"
     CDS             64674..65249
                     /gene="gmhB"
                     /locus_tag="ACHX5T_000133"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR8465512.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            65613..>65883
                     /locus_tag="ACHX5T_000134"
     rRNA            65613..>65883
                     /locus_tag="ACHX5T_000134"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15573 a        16336 c        18173 g        15801 t
ORIGIN      
        1 aatgctcttg tatgtaagac ttcacgcaac gtctccccat caggaaacgc cagctccggt
       61 gcaatctcaa acagcggcca cagcataaat ccacgattct tcatatcgta gtgcggaacg
      121 gtcaggcgtt cggtatttat cacttcatta ccaaacagca tgatatcgag atccagcgtg
      181 cgtggtcccc agcgttcagc tttacgaaca cgaccctgtt gcagttctat gcgctgtgta
      241 tggttgagta actcttccgg ggcgggcgaa gtttcaagcg ccacggcggc gttgaggtaa
      301 tcgggttgat cttgcggccc cagcggtggg gtgcggtaaa acgaagaaac ggcaagaatg
      361 cggctttcag ggatatcgcc taatgctttc agggcagcat tgacctgctc cagcggagag
      421 gccagattgc tgcctatggc aatatacgcc actgtcatgt ggtaccctca cgacgcggtg
      481 cgcgtttgcg tggacgacga gtacgacgac gcggtgacgg ttcttcatcc agctcgttaa
      541 gcatcccttt ttgatctggt ggtgcggaaa cctggaactc accccaccat ttcaccagac
      601 gctgcagttc agcattacgt tcaacttcag ctcgcaaggc caacaggtca taagccgcac
      661 ggaacttagg atgctccagc agtttccatg cgcgtttacc ctgacgacgg gacatacgca
      721 actgcaactg ccagatatcg cgggttaacg tcgtcagacg tttcgggatt gccagtgaac
      781 ggcaggcttc gtccagcacg tcgttcatcg ccagcgcgaa agcgtcgtga taggtcaggc
      841 cgctttcctg ggcgatcttc tgtgccgtct ccagcagtgg gtaccagaac atggcggcaa
      901 acaagaacgc cgggttcacg cgcatatcgt tatggatacg cgtatcggta ttcttcagca
      961 cctgttcaat gatccgctcc atcgggctgt cgccattttc cgtgaagtag cgggtaatgg
     1021 tcgggaacag cggctggaac agatgatatt cacacaacag cttataggtt tcgtaaccgt
     1081 agcccgcttg tagcagttta agcgattctt caaacaggcg tgccggtggg atatcgttca
     1141 gcagggtagc gaggcgaggg atcggttctg cggtttccgg gctgatgcgc atacccaatt
     1201 tggcggcaaa acgtaccgcg cgcagcatac gtaccggatc ttcacggtag cgcgtttccg
     1261 ggttaccaat cagacggata acgccgtcct tcagatcctt cataccacca acgtaatcac
     1321 ggacggtaaa atccgctacg ctgtaataca ggctgttgat agtgaaatcg cggcgctggg
     1381 cgtcttcttc gatggagccg aaaatgttgt cgcgcagcaa catgccgttt tgcccgcgtt
     1441 gggaggtcgt gcggtcgctg acgttacctt cgtggtgtcc acggaaggtc gcaacttcga
     1501 taatctccgg gccaaacatt acatgagcca gacggaaacg gcgacccacc aggcggcagt
     1561 tacggaacag tttgcgcacc tgctcaggcg tggcgttagt ggttacgtca aaatctttcg
     1621 gctttttgcc aagtaacagg tcgcgcacgc cgccgccaac cagccaggct tcgtatcccg
     1681 ctttgttgag cctgtacatt accttcaggg cattttcact gatatctttg cgggaaatag
     1741 catgctgctc acgcgggatc accgtcacct gtggacgggc gactgcctgt tcagcctcgc
     1801 tttcctcgcg gcttagcacc ttgcggcaaa aattagcgac tcgggtaaaa atagtacacc
     1861 tcggtagtgt caaacatcat tcaggacaaa aaaatagcgg ctaatcatag ctcagcatga
     1921 cgcatttgag aatgttgaat ttacaattgc cgactcgggc acggcggtca gcatccagtt
     1981 tttgacggct gactgaagga tttgctcgac gctgaaatcc tgccagtgtg cttctgcctg
     2041 ctgccccaga aattgaagtg ccgcgattag taccgggcgt ggatcgcctt tcggcaacgc
     2101 aggcgcatga ttctgcttgg aaagtttagc gccttgtgga ttaagcgcca gcggcagatg
     2161 aatgtaatct ggcactttcc agccaaaaag ctggtacagc gggatttgcc ttactgttgg
     2221 ttcaatcaga tcagccccac gcactatttc tgtaacgccc tggaaatgat catcaaccac
     2281 aacagccagg ttgtaggcga acaacccatc acggcgatga atgataaaat cttcccgtgc
     2341 cagtttttcg tcggcgtgaa taatgcctcg cagcaggtca gtaaattgcg tgaccggatg
     2401 ctgctggcgg atacgcactg cggcgttgtc tggtccatga tgcaacaccc ggcaatgacc
     2461 gtcgtaaata ccgccaatgc tttgaatacg cgcacgcgta caggtgcagt aataacttag
     2521 tccttgttca tgtaaccagg cgagtgcttc acgataggcg tcgtgacgtt gcgattgcca
     2581 gagaacatcg ccgtcccagt gcagaccgta atgttccagc tggcgcagga tagtttctgc
     2641 ggcaccggga acttcacgag gcgggtcgat atcttctatg cgtaccagcc agcgaccttg
     2701 ccgggcgcga gcctgcaaat agctgccgag cgcggcgatc agagagccaa aatgaagctc
     2761 gccggaagga gagggggcga agcggccaat atactgtgtg tctgtcatct ctttgaacaa
     2821 aaaataaggc gggagcattt cccgcctgtg gtaaacgtga tggaacggct gtaattagcc
     2881 agccatctgt ttttcgcgaa tttcagccag cgttttgcag tcgatgcaca gatcggctgt
     2941 cgggcgcgct tccagacggc gaataccaat ttcaacaccg caggattcgc agtagccgaa
     3001 atcttcgtct tccacttttt tcagcgtctt ctcgatcttt ttgatcagct tacgctcgcg
     3061 atcgcggtta cgcagttcga ggctgaactc ttcttcctgg gctgcacggt ctaccgggtc
     3121 cgggaagttg gctgcttcat cctgcatatg tgtaacggtg cgatcgactt catccctgag
     3181 ttgattacgc catgcttcca gaatacgacg gaagtgcgcc agctgggctt cattcatata
     3241 ctcttcgccc ggcttctcct gatatggttc caccccagcg atggcgagaa tactcaggga
     3301 cgatgtttta cggttttgcc cttcttgcat gttgcttctc cttaacacgc actatcgatc
     3361 cccatgttcg ggggaaaaat gaggccgcta taaatagcag atgcttttcc ggatagcaat
     3421 tatctaaacg taacacttga caactgtgtg aggaaaagcg tatttgcgca cgcgaccaga
     3481 atgtaaatta accagttact tactttacta caatgtaacc ggcagtgatt ttttaagagc
     3541 catgccttca gcagaaattt ccgctttgta agccagaatt tctacccccc tctgttgagc
     3601 ttctgacaat agttgcgcgt atttctcatc gatgtggcgc gcgggtgaaa accgtgtaat
     3661 ggctgaatgc agcacggcga aaaaaataac cgcacgctgg ccttcagccg ctacgctcat
     3721 caactcccga aggtgtttct gacctcgttc agtgaccgca tcgggaaaat atccctgttc
     3781 gttctccgct aacgtaaccg atttcacttc aatatagcag tctggacgcg aatccgcctg
     3841 caacataaag tcaatacggc tgcgttcgga gccgtatttt acttcgcttt tcagcgagct
     3901 atagcctgac agttctgaaa ttgattcatt aaggatagcc tctttcgtca acctgttagc
     3961 ccaaagcgta ttgacgcaaa taaatgcgcc gctctggctt tgagttaatt cccaggtgtg
     4021 tgggtatttc cgtttggtgt tgtctgaagt cgaataccag acggtatcgc caggcgttgc
     4081 acaaccggtc atcgcacccg tattcgggca gtgtagcgtt aattcgcgac catcgggtgt
     4141 gatcacatcg gctaaaaaac gtttgtaacg ctgaattagc gtcgcgcgct gtagaggggg
     4201 agaaaattcc attgcgacaa tccttgttat tgcgttagcg cccagcgttt tagcggcgtg
     4261 tagcgtgtgc gtccacgggc aaacgaggag gcgtaaaggg tgaactccgt caccgcataa
     4321 gaccagttaa aacccggcgg cgggaatgtc accgcctcgc tggcgtcgcg caataaggta
     4381 atatgtggat gaaacggacg attgctttga aaacaaccgc tgcgggcagc ctgtgaacgg
     4441 agcatattcg ccagctggat taagccgcgt ggaggctgac gcatccctaa ccacaccaca
     4501 cgcgaacgca gccattgtcc ggcgtcatca agcgtgagtg tgaaaccagg ttgacgaatc
     4561 cgtccggcta aaagagaaag cgccttctct ttctctgcgc tcacttcgcc taaaaatgcc
     4621 agagtcaggt gcaaattatc ggcggcgacc ggacgtcccg cctcaggtgg gaagtgtgtg
     4681 gcgcgccaat ggataatctg ttcgcggatt tctgcaggta agtcgatagc aaagaacaga
     4741 cgttgcggtt cagacatgtg aggcactcgg ttatgaatta ccgcgatgct acaatgtggc
     4801 gcgaagaatg ttaaccctct ggagcgtttt gtgtcgtcgt tgcccgttgc tgccgtctta
     4861 cctgaattac ttaccgctct cgattgtgcg ccgcaggtat tattaagtgc gccgaccggg
     4921 gccgggaaat caacctggct gccgctgcaa ctgctggcgc atcccggcat taacgggaaa
     4981 attatcctgc tggagccgcg tcgtctggcg gcgcgtaacg tcgcgcaacg gctggcggag
     5041 ctgcttaacg aaaagccagg cgataccgtt ggctaccgga tgcgtgcgca aaactgcgtc
     5101 gggctgaata cccgcctgga agtggttacc gaaggcgtgc tgacgcggat gatccagcgt
     5161 gacccggaac tgagcggtgt tggattggtg atccttgatg agtttcatga gcgcagtttg
     5221 caggcggatt tggcgttggc gctgttactc gatgtgcaac aaggtctgcg tgatgacctt
     5281 aaactgctga ttatgtcggc tacgctggac aatgaccgct tgcagcaaat gctgccggaa
     5341 gcgcccgtcg tcatctcaga agggcgctcg tttccggttg aacgccgtta tttaccgctg
     5401 cccgcgcatc agcgttttga cgaagccgtt gcggtagcca ccgccgaaat ggtgcgtcag
     5461 gaaagtggat cattactgtt atttttacct ggcgtcggag aaattcagcg cgtgcaggaa
     5521 caactggctt cgcgcatcgg cagtgatgtg gtgctctgcc cgctgtatgg cgtgttgtca
     5581 ctgaacgatc agcgaaaagc gatcctcccg gcaccgcaag ggatgcgcaa agtggtgctg
     5641 gcgaccaata ttgctgaaac cagtttaacc atcgaaggta ttcgtctggt ggtggattgt
     5701 gcccaggagc gtgtggcgcg ctttgatccg cgcacggggc ttacgcggtt gattactcaa
     5761 cgcgttagtc aggcatccat gacgcagcgt gccgggcgcg ccgggcgtct ggagccgggt
     5821 atcagcctgc atttaatcgc caaagaacaa gcagaacgcg ccgcggcgca aagtgagccg
     5881 aagatcttac aaagcgatct ttccggtttg ctgatggaat tactgcaatg gggatgcagc
     5941 gatccggcgc agatgagctg gctggatcaa ccgccaacgg tgaatctact ggccgcgaaa
     6001 cgcctgttac aaatgctggg ggcgctggag ggtgaacggc ttagtgcgca agggcaaaaa
     6061 atggcagcgc tgggtaacga tccgcgttta gcggcaatgc tggttaacgc aaagagcgat
     6121 gacgaagctg ctaccgcggc aaaaattgcc gccattctcg aagagccgcc acggatgggc
     6181 aatagtgacc tgggcgtggc gttttcgcgc aatcaaccag cctggcagca acgtagtcag
     6241 caactgttaa aacgcttaaa cgtacgcggc ggtgaggcag acagttcgct tatcgcgccg
     6301 ctacttgccg gggcgtttgc cgatcgcatt gctcatcgcc gtgggcaaga tggacgctat
     6361 caactggcaa acagcatggg agcgatgctc gatgccgacg acgcgctaag ccgccacgaa
     6421 tggttgatcg caccgttatt attgcagggc agcgcctcgc cggatgcgcg gattttactg
     6481 gcgctaccgg tcgatattga tgaattagta caacgctgtc cgcagctggt gcagcaatct
     6541 gacaccgtgg aatgggatga cgcgcaaggt acgctgaaag cctggcgtcg gctacaaatc
     6601 ggtcagttga cggtgaaagt gcagccgctg gcgaaaccgt cagaagacga gttgcatcag
     6661 gcgatgctta atggcatccg tgataaaggt ttaagcgtgc tcaactggac ggcggaagcg
     6721 gaacagctac gcttgcgttt gttatgcgcc gcaaagtggt tgccggaata tgactggcca
     6781 gcggttgatg atgaaagttt gttggcgacg ctggaaacgt ggctgctgcc acatatgacc
     6841 ggcgtacatt cactacgcgg cctgaaatca ctcgacattt atcaggcact acgcggatta
     6901 cttgattggg gaatgcagca acgtctggat agtgaattgc ctgcgcatta cactgtgccg
     6961 acgggaagcc ggatcgccat tcgttatcat gaagataacc cgcccgcgct ggcggtgaga
     7021 atgcaggaga tgtttggtga ggccactaat ccgacgatcg cccaggggcg cgtgccgctg
     7081 gtgctgaagt tgctttcccc tgcccaaagg ccgctgcaaa tcacgcgtga tttgggggcc
     7141 ttctggaaag gggcgtatcg tgaggtgcaa aaggagatga aagggcgtta tcccaaacac
     7201 gtctggccgg acgacccggc aaacacggca ccgacgcgac ggacgaaaaa gtattcgtaa
     7261 ggttggtttt ctccctctcc ctgtgggaga gggccggggt gagggcatca gcgcgcacgt
     7321 tcaccctcac cctaaccctc tccctcaagg gagaggggac cgatcgagcg caaattttga
     7381 gagatatctt cttctgtctt gtaacagaag aacagaaaat cgggcttttg cgcctgaata
     7441 ttgcggagaa aaagcatggc cgggaatgac cgcgagccaa ttggacgcaa agggaaaccg
     7501 acgcgtccgg tcaaacaaaa ggtaagccgt cgtcgttacg aagatgacga tgattacgac
     7561 gattatgatg actatgagga tgaagaaccg atgccgcgca aaggtaaggg caaaggcaaa
     7621 gggcgtaagc ctcgtggcaa acgcggctgg ctatggctac tgctaaaact ggctatcgtt
     7681 tttgccgtgc tgatcgccat ttacggcgtt tatctcgatc aaaaaattcg tagccgtatt
     7741 gatggcaagg tctggcaact gcccgcggca gtttatggcc gaatggtcaa ccttgaacca
     7801 gatatgacca tcagcaagaa cgaaatggtg aagctgctgg aggcaacaca gtatcgtcag
     7861 gtgtcgaaaa tgacccgtcc tggcgaattt accgtgcagg ccaacagcat tgagatgatc
     7921 cgccgtccgt ttgatttccc ggacagtaaa gaagggcagg tgcgtgcgcg tctgaccttt
     7981 gatggtgatc atctggcgac catcgtcaat atggagaaca accgtcagtt cggtttcttc
     8041 cgtcttgatc cgcgtctgat caccatgatc tcctcgccaa acggtgagca gcgtttgttt
     8101 gtgccgcgca gtggtttccc tgatttgctg gtggatactt tgctggcgac agaagaccgt
     8161 catttttacg agcatgatgg aatcagtctc tactccattg ggcgtgcggt gctggcaaac
     8221 ctgaccgcag gtcgcacggt gcagggtgcg agtacgctga cgcaacagct ggtgaaaaac
     8281 ctgttcctct ccagcgagcg ttcttactgg cgtaaagcga acgaagctta catggcgctg
     8341 atcatggacg cgcgttacag caaagaccgt attcttgagc tgtatatgaa cgaggtgtat
     8401 ctcggtcaga gcggcgacaa cgaaatccgc ggcttcccgc tggcaagctt gtattacttt
     8461 ggtcgcccgg tagaagagct aagcctcgac cagcaggcgc tgttagtcgg tatggtgaaa
     8521 ggggcgtcca tctacaaccc gtggcgtaac ccgaaactgg cgctggagcg acgtaatctg
     8581 gtgctgcgtc tgctgcaaca gcaacagatt attgatcaag aactctatga catgttgagt
     8641 gcccgtccgc tgggggttca gccgcgtggt ggggtgatct ctcctcagcc agcctttatg
     8701 cagctggtgc gtcaggagtt gcaggcaaaa ctgggcgata aggtaaaaga tctctccggc
     8761 gtgaagatct tcactacctt tgactcggtg gcccaggacg cggcagaaaa agccgccgtg
     8821 gaaggcattc cggcactgaa gaaacagcgt aagttgagcg atcttgaaac tgcgattgtg
     8881 gtcgtcgacc gctttagtgg tgaagttcgt gcgatggtcg gaggttctga gccgcagttt
     8941 gcgggctaca accgtgcgat gcaggcgcgt cgttcgattg gttcccttgc aaaaccagcg
     9001 acttatctga cggctttaag ccagccgaaa atctatcgcc tgaacacgtg gattgccgat
     9061 gcgccaatcg cgctgcgtca gccgaatggc caggtctggt caccgcagaa cgatgaccgt
     9121 cgttatagcg aaagcggcag agtgatgctg gtggatgcgt tgacacgttc gatgaacgtg
     9181 ccgacggtaa atctggggat ggcgctgggg ctgcctgcgg ttacggagac ctggatcaaa
     9241 ctgggcgtac cgaaagatca gttgcatccg gttccggcaa tgctgctggg ggcgttgaac
     9301 ttaacgccaa tcgaagtggc gcaggcattc cagaccatcg ccagcggtgg taaccgtgca
     9361 ccgctttctg cactgcgttc ggtaatcgcg gaagatggta aggtgctgta tcagagcttc
     9421 ccgcaggcag aacgcgctgt tccggcgcag gcggcgtatc tgacactatg gaccatgcag
     9481 caggtggtac aacgtggtac gggtcgtcag cttggggcga aatacccgaa cctgcatctg
     9541 gcagggaaaa cagggactac caacaataac gtagatacct ggtttgcagg cattgacggc
     9601 agcacggtga ccatcacctg ggtaggccgt gataacaacc agccgaccaa actgtatgga
     9661 gccagcgggg caatgtcgat ttatcagcgt tatctggcta accagacgcc aacgccgctg
     9721 aatcttgttc cgccagaaga tattgcagat atgggcgtgg actacgacgg caactttgtt
     9781 tgcagcggtg gcatgcgtgt cttgccggtc tggaccagcg atccgcaatc gctgtgccag
     9841 cagagcgaga tgcagcagca gccgtcaggc aatccgtttg atcagtcttc tcagccgcag
     9901 caacagccgc aacagcaacc tgctcagcaa gagcagaaag acagcgacgg tgtagccggt
     9961 tggatcaagg atatgtttgg tagtaattaa catctaagcg tgaaataccg gatggcgagt
    10021 tgccatccgg taaaataaca tcccatctaa gatattaacc ctttcttttc atctggttgt
    10081 ttattaaccc ttcaggaacg ctcagattgc gtaccgcttg cgaacccgcc agcgtttcga
    10141 atattatctt atctttataa taatcattct cgtttacgtt attattcact ttacatcaga
    10201 gatataccaa tggcgcgttc caaaactgct cagccaaaac actcactgcg taaaatcgca
    10261 gttgtagtag ccacagcggt tagcggcatg tctgtttatg cacaggcagc ggttgaaccg
    10321 aaagaagaca ctatcaccgt taccgctgca cctgcgccgc aagaaagcgc atgggggcct
    10381 gctgcaacta ttgcggcgcg gcagtcagct accggcacta aaaccgatac gccgattcaa
    10441 aaagtgccac agtctatttc tgttgtgacc gccgaagaga tggcgctgca tcagccgaag
    10501 tcggtaaaag aagcgcttag ctacacgccg ggtgtctctg ttggtacgcg tggcgcatcc
    10561 aacacctatg accacctgat cattcgcggt tttgcggcag aaggccaaag ccaaaataac
    10621 tatctgaatg gcctgaagtt gcagggcaac ttctataacg atgcggtcat tgacccgtat
    10681 atgctggaac gcgctgaaat tatgcgtggc ccggtttccg tgctttacgg taaaagcagt
    10741 cctggcggtc tgttgaatat ggtcagcaag cgtccgacca ccgaaccgct gaaagaagtt
    10801 cagtttaaag ccggtactga cagcctgttc cagactggtt ttgactttag cgatgcgttg
    10861 gatgatgacg gtgtttactc ttatcgcctg accggtcttg cgcgttctgc caatgcccag
    10921 cagaaagggt cagaagagca gcgttatgct attgcaccgg cgttcacctg gcgtccggat
    10981 gataaaacca atttcacctt cctttcttac ttccagaacg agccggaaac cggttattac
    11041 ggctggttgc cgaaagaggg aaccgttgag ccgctgccga acggtaagcg tctgccgaca
    11101 gactttaatg aaggggcgaa gaacaacacc tattctcgta atgagaagat ggtcggctac
    11161 agcttcgatc acgaatttaa cgacaccttt actgtgcgtc agaacctgcg ctttgctgaa
    11221 aacaaaacct cgcaaaacag cgtttatggt tacggcgtct gctccgatcc ggcgaatgct
    11281 tacagcaaac agtgtgcggc attagcgcca gcggataaag gccattatct ggcacgtaaa
    11341 tacgtcgttg atgatgagaa gctgcaaaac ttctccgttg atacccagtt gcagagcaag
    11401 tttgccactg gcgatatcga ccacaccctg ctgaccggtg tcgactttat gcgtatgcgt
    11461 aatgacatca acgcctggtt tggttacgac gactctgtgc cactgttcaa tctgtacaat
    11521 ccggtgaatc ccgatttcga cttcaatgcc aaagatccgg caaactccgg cccttaccgc
    11581 attctgaata aacagaaaca aacgggcgtt tatgttcagg atcaggcgca gtgggataaa
    11641 gtgctggtca ccctaggcgg tcgttatgac tgggcagatc aagaatctct taaccgcgtt
    11701 gctgggacga ccgataaacg tgatgacaaa cagtttacct ggcgtggtgg tgttaactac
    11761 ctgtttgata atggtgtaac accttacttc agctatagcg aatcgtttga accttcttcg
    11821 caagttggga aggatggtaa tattttcgca ccgtctaaag gtaagcagta tgaagtcggc
    11881 gtgaaatatg taccggaaga tcgtccgatt gtagttactg gtgccgtgta taatctcact
    11941 aaaaccaaca acctgatggc ggaccctgag ggttccttct tctcggttga aggtggcgag
    12001 atccgcgcac gtggcgtaga aatcgaagcg aaagcggcgc tgtcggcgag tgttaacgta
    12061 gtcggttctt atacttacac cgatgcggaa tacaccaccg atactaccta taaaggcaat
    12121 acgcctgcac aggtgccaaa acacatggct tcgctgtggg ctgactatac cttctttgac
    12181 ggtccgcttt caggtctgac gctgggcacc ggtggtcgtt atactggctc cagctatggt
    12241 gatccggcta actcctttaa agtgggaagt tatacggtcg tggatgcgtt agtgcgttat
    12301 gatctggcgc gagtcggcat ggcgggctcc aacgtggcgc tgcatgttaa caacctgttc
    12361 gatcgtgaat acgtcgccag ctgctttaac acttatggct gcttctgggg cgcagaacgt
    12421 caggtcgttg caaccgcaac ctttcgtttc taatttctct tttggggcac ggatttccgt
    12481 gcccatttca caagttggct gttatgcagg aatacacgaa tcattccgat accacttttg
    12541 cactgcgtaa tatctccttt cgtgtgcccg ggcgcacgct tttgcatccg ctgtcgttaa
    12601 cctttcctgc cgggaaagtg accggtctga ttggtcacaa cggttctggt aaatccactc
    12661 tgctcaaaat gcttggtcgt catcagcctc cgtcagaagg ggagattctt cttgatgccc
    12721 agccgctgga aagctggagc agcaaagcgt ttgcccgcaa agtggcttat ttgccgcagc
    12781 agcttcctcc ggcagaaggg atgaccgtgc gtgaactggt ggcgattggt cgttacccgt
    12841 ggcatggcgc gctggggcgc tttggtgcgg cagatcgcga aaaggtcgag gaagctatct
    12901 cgctggttgg cttaaaaccg ctggcgcatc ggctggtcga tagtctctct ggcggcgaac
    12961 gtcagcgggc gtggatcgcc atgctggtgg cgcaggatag ccgttgtctg ttgctcgacg
    13021 aaccgacttc agcgctggat atcgcccacc aggttgatgt gctggcgctg gtgcaccgtt
    13081 taagtcagga acgtggcctg acggtcattg ccgtgttgca cgatatcaat atggcggcac
    13141 gctactgtga ttatctggtc gccctgcgca gtggtgaaat gattgctcag ggaacgcctg
    13201 cggaaattat gcgcggcgaa accctcgaaa tgatttatgg catcccgatg ggtattttgc
    13261 cgcatccggc gggtgctgca cctgtgagtt ttgtttattg atgagcggct tacctcttat
    13321 ttcgcgccgt cgactgttaa cggcgatggc gctttctccg ttgttatggc agatgaatac
    13381 cgcccacgcg gcggctattg accccaatcg tattgtggcg ctggagtggt tgccggtgga
    13441 attactgctg gcgctcggca tcgtgcctta cggcgtggcg gataccatca actatcgcct
    13501 gtgggtcagc gaaccaccat tgccggactc agtgattgac gtcggtttgc gtacagaacc
    13561 taaccttgaa ctgctgaccg aaatgaaacc gtcgtttatg gtctggtcgg caggatatgg
    13621 cccttcatca gaaatgctgg cacgtattgc gccgggtcgc ggatttaact tcagtgacgg
    13681 caaacatccg ttggcgatgg cgcgtaaatc gctgacggaa atggcagatt tacttaacct
    13741 gcaaagcgca gcggaaacgc atttagcgca atacgaagac tttatccgca gcatgaaacc
    13801 ccgctttgtg aagcgtggtg cacgcccgtt actgctgacg acgcttatcg atccgcgcca
    13861 tatgctggtc ttcggtccaa acagcttgtt ccaggaaatt ctcgatgagt acggcatccc
    13921 aaatgcctgg caaggggaaa ccaacttctg gggcagtacc gccgtcagta tcgatcgtct
    13981 ggcagcgtat aaagacgttg atgtgctctg ttttgatcac gacaacagca aagacatgga
    14041 tgcgctaatg gcaaccccgc tgtggcaggc catgccgttt gtccgtacag ggcgctttca
    14101 gcgcgtaccc gcagtctggt tttatggtgc aacgctttcg gcaatgcact ttgtgcgtgt
    14161 tctggataac gccatcggag gtaaagcgtg agtaaacgaa ttgcgctttt cccggcgtta
    14221 ttgctggcgc tgttagtgat tgtcgctacg gcgctcacct ggatgaactt ctcacaggcg
    14281 ctgccgcgca gtcagtgggc gcaggccgcc tggtcgccgg atattgacgt catcgagcag
    14341 atgatttttc actacagctt gttgccgcgt ctggcgattt cgctgctggt gggcgcgggc
    14401 ctggggctgg tgggcgtgct gtttcagcaa gtactgcgta acccgctggc ggaaccgacg
    14461 acgttgggcg ttgcaacagg cgcgcaactg gggattaccg tcaccacgct ctgggcgatc
    14521 cctagcgcga tggcgagcca gtttgctgcg ctggcagggg cttgcgttgt tggcttaatt
    14581 gtctttggcg tcgcgtgggg gaaacggctg tcgccggtaa cgctgatttt cgcggggttg
    14641 gtagtgagcc tttattgcgg cgcaatcaat cagttactgg ttatcttcca tcatgaccag
    14701 ttgcaaagca tgtttctgtg gagcactgga acgctgacgc aaaccgactg gggcggtgtt
    14761 gagcgtttat ggccgcagtt gctgggcggt gtgatgctga cgttgctgct acttcgtccg
    14821 ttaaccctga tggggcttga tgatggcgtg gcgcgcaatc tcgggctggc cttgtcgctt
    14881 gcgcgtctgg cggcgctgtc gctggcgatt gtcatcagtg cgctgctggt gaacgctgtg
    14941 gggattatcg gctttatcgg gttgttcgcg ccgctgctgg caaaaatgct gggggcgcgg
    15001 cgtctgctgc cacgactgat gctggcgtcg ttgattggtg cgctgatcct ctggctttcc
    15061 gatcaaatca tcctctggct gactcgcgtg tggatggaag tgtccaccgg ttcggtcact
    15121 gcgttgatcg gtgcgccgct gctactgtgg ctgctgccgc gtttacgcag cattagcgcg
    15181 ccggatatga aggttaacga tcgtgtcgcg actgaacgcc aacatgtgct ggcgtttgcc
    15241 ctcgcgggcg gcgtgctgtt gttgatggct gtggtggtgg cgctgtcgtt tggtcgtgat
    15301 gcgcacggct ggacgtgggc gagcggggcg ttgctcgagg atttaatgcc ctggcgctgg
    15361 ccgcgaatta tggcggcgct gtttgcgggc gtcatgctgg cggtagcggg ctgtattatt
    15421 cagcgactga ccggcaaccc gatggcaagc ccggaagtgc tggggattag ctccggcgcg
    15481 gcgtttggtg tggtgttaat gctgtttctg gtaccgggta atgcctttgg ctggttgtta
    15541 cccgcaggca gtctcggggc ggcggtgacg ctgttgatca ttatgatcgc cgccggacgc
    15601 ggtggatttt ccccacaccg catgttactg gcggggatgg cgttaagtac cgcgttcacc
    15661 atgcttttga tgatgttgca ggcaagtggt gacccgcgaa tggcgcaagt gctgacctgg
    15721 atttccggtt cgacctacaa cgcgaccgat gctcaggtct ggcgcaccgg aattgtgatg
    15781 gtgattttgc tggcgattac tccgctgtgc cgccgctggc tgaccatttt accgctgggt
    15841 ggtgataccg cgcgggcggt agggatggcg ctgacgccga cgcgaattgc gctgctgctg
    15901 ttagcggctt gcctgacggc gaccgcgacg atgactattg gaccgttgag ttttgttggt
    15961 ttaatggcac cgcatattgc gcggatgatg ggctttcgac ggacgatgcc acacatcgta
    16021 atttcggcgc tggtgggtgg tttactgctg gtgttcgctg actggtgtgg gcggatggtg
    16081 ctgtttccat tccagatccc ggcggggctg ctgtcgacct ttatcggtgc gccatatttt
    16141 atctatttgc tgagaaagca gagccgttaa tagaatgcct cgcccggtgg gcgaggcaaa
    16201 attatcacaa cttcgcgaac acccgacgtg cagcatcgat ggtgttattg atatcttcca
    16261 tgctgtgcgc cacagacata aagcctgctt caaacgctga cggtgccagg taaacacctt
    16321 catccagcat catatggaag aaacgcttaa aacgctcgac gtcacaggcc atcacatcct
    16381 gatagcacgt cacggactcg gcgtcggtaa agaagatacc gaacatacca ccgacgtggt
    16441 taacgaccag cggaattccc gcttcttctg ccgcttccag cagaccttct gccagacgtg
    16501 ttgtcagctc atccagcgtt tcgtgaatgc ccggctgcgc gacttcattc agacaggcga
    16561 aacccgctgc catcgcaatc gggttaccgg aaagcgtacc cgcctgatag accggacctg
    16621 tcggggccag cgcatccatt acatcacgac gaccaccgaa tgcgcctacc ggcattccac
    16681 cgccgatgat ttttccgagg caggttaaat ctggcactac ggcgaaataa tcctgtgcgc
    16741 cagctagcgc tacgcggaaa ccggtcatca cttcatcgat gatcagcaac gcgccaaatt
    16801 cgtcgcacag cgcacgcaga cctggcagga actctggcag cggcggaaca cagttcatat
    16861 tgcctgccac cggctcgacg ataatacagg caatctcttg cgggtattgc tcaaacgcgg
    16921 cgcgtacaga ggccagatcg ttataagtac aggttaaggt atgtttggcg aaatctgccg
    16981 gaacgcccgg cgagtttggc tggcctaacg tgagtgcgcc agaaccggct ttcaccagca
    17041 ggcagtcagc gtgaccgtgg taacaacctt caaatttaat gattttgtcg cgaccggtaa
    17101 aaccacgggc caggcggatg gcgctcatcg tcgcctcggt gccggagttc accatgcgca
    17161 ccatatccat ggtcgggacc agttcggtca ccagttgcgc cattttcact tccatttcgg
    17221 tcggtgcacc aaagcttaaa ccacgctcgg cggcttcaat cacggcattg cggatcgccg
    17281 gatggttatg gcccagcacc atcggccccc aggaaccgac ataatcgata taggctttgc
    17341 catcaacatc gtacagataa gcgccgtccg ctttttcgat aaacagtgga gtgccgccca
    17401 cgccagtaaa ggcgcgaaca ggggagttca caccgccagg gatcagctcg cgcgctgcgc
    17461 tgtaaagatt ttcagactta ctcatggagg gttcctgatt cgtagaaaaa gtgaatggct
    17521 gctattccat gttattcata acaagttaaa tactcgtcaa acatcaggct gcttgtactg
    17581 gtgcaatcct gatttcttag agtataaaag ttttgtgcat ttgaaacatt acgctttgca
    17641 aaggattttc atggaacgtg cgagtaaaat gccgtcatct tatttgtatg accaataagt
    17701 gatcattgga tgaaaactga tactccctct ttagaaacac cgcaggccgc gcgcctgcga
    17761 cgcagacaac tgattcgcca acttcttgag cgcgataaaa ccccgttagc cattttgttt
    17821 atggcggcag tcgtcggcac gcttgtcggg ctggctgcgg ttgcttttga caaaggtgtc
    17881 gcctggttgc agaaccaacg tatgggggcg ctggtacata ctgctgataa ttatccgcta
    17941 ctgttaaccg tcgcttttct ctgttcggcg gtgctggcga tgttcggcta ctttttggtg
    18001 cgcaaatacg cgcccgaagc agggggatcg gggatcccgg aaattgaagg ggcgctggaa
    18061 gatcaacgtc ctgttcgctg gtggcgtgta ttgccggtga aattctttgg cgggctgggg
    18121 acactcggcg gaggcatggg gctagggcgc gaaggaccaa ccgtgcagat cggcggcaat
    18181 atcagtcgca tggtgcttga tattttccgc ctgaaaggtg atgaagctcg ccatacgctg
    18241 ctggcaaccg gtgctgctgc ggggctggct gcggccttta acgcgccgct ggcgggtatt
    18301 ttgtttatta tcgaagagat gcgtccgcag tttcgctata cgttaatttc gattaaagcg
    18361 gtatttattg gcgtcattat gtcgaccatt atgtaccgga tttttaatca tgaagttgcg
    18421 ttgattgacg tcggtaaact ttctgatgcg ccgcttaata cgctgtggct ttatctgatc
    18481 ctcggtatta tttttggcat tttcggccct atttttaata aatgggtgct ggggatgcag
    18541 gatttgctgc accgtgtgca cggcggcaat attaccaaat gggtactgat ggggggggcg
    18601 attggcggtc tgtgtggatt gctggggttt gtggcaccag caacgtcggg cggcggtttt
    18661 aacctgattc ctatcgctac cgcggggaat ttcagcatgg gaatgctggt gtttatcttc
    18721 gtcgctcggg tcattaccac cttactctgc ttctcttccg gcgcgccggg cggtattttt
    18781 gccccgatgc tggcgctggg tactgtgctg ggaaccgctt tcggaatggt tgccgttgag
    18841 ctgtttccgc aatatcacct ggaggcgggg acgtttgcta ttgccggaat gggggcatta
    18901 ctggcggcat ctattcgcgc gccgttaacg gggatcattc tggttctgga gatgaccgat
    18961 aactaccagc tcattttgcc aatgattatt accggtcttg gcgcaacact attagcgcaa
    19021 tttaccggcg ggaaacctct atactcggcg attcttgcgc gcacgctggc aaaacaggaa
    19081 gctgagcaac tggcgcgaag caaggccgca tcagccagcg agaatacttg aacgaaatac
    19141 cagggtatta gataatggcg attattattg ggttagaatt tgcccgattg ccgatgtcgt
    19201 ttggagcaaa atatgagtga tgacgtagca ctgccgctgg agtttaccga cgcagcagcc
    19261 aacaaagtta aaagcctgat cgctgacgaa gataacccga atctgaaatt acgcgtgtat
    19321 atcaccggtg gcggttgcag cggcttccag tatggtttca cctttgatga tcaggtgaac
    19381 gaaggcgata tgaccatcga aaaacagggc gttggcctgg tggttgatcc gatgagcctg
    19441 caatatctgg tcggcggttc cgttgactac accgaaggac tggaaggttc tcgtttcatc
    19501 gtgaccaacc cgaacgcgaa aagcacctgc ggttgcggtt cttcctttag catctaatct
    19561 gttgttcatg tgccggataa actatccggc acatcttctc tcttcaacgc ccattctcat
    19621 ccagcgcaaa cgtcggtagc ttaagatgcc aacgaatagc cgccagccga atcaatagcg
    19681 tcacgaccat gcccatcata ctggctgttt ccagtggtac ggaaaatgtg taataagccg
    19741 tagcgtggac aataccgccg ataatacagg cagttgcgta gatttctgta cgtaaaatca
    19801 tggggatttc gcggaccagt acatcacgaa tgatcccgcc gccaacgcca gtaatgacgc
    19861 ccatacaaac cgcgattaac ggaccggctt ccgcattaaa ggctttattc acgctaatgc
    19921 cgacaaacac cgccagacca acggcgtcca gcaccggcaa catccatttt ggtaagcgtc
    19981 ttggctggcg caccagcacg atggtcagca tgctggtgac catggcaacg accagatcgg
    20041 tgggatcttt cacccaaaat accgggccgt gatccagcgc catgtcgcga attgttccgt
    20101 cgcctactgc ggtaaccacg cccagtacca gaacaccaaa agggtccata cgcaattttc
    20161 cggctaacaa aacgccgtag atggcaaata ccgctgtgcc gactatatcc agccaataga
    20221 cgagcattgt tcaatcccca ctgagcacct gaaaaggtca ggcactaatc tacctgtgaa
    20281 agcgcattac agagctgttg tgctgcgagg ataatacgtg ggcttgcacg ttcaaaccag
    20341 tcactcgtga gaggaataac gggaattttg agctgttcac cccagtattg tttgatttta
    20401 ggaatttggt ccggtccgcc tgtaatgaca atagcctgtg gcgagcgtgc taacacctgt
    20461 tcgcggctaa cttgcggcca gggaacccgg ctgtctttaa agatgttttc tccgccacaa
    20521 acttcgagta cctggttctg aatcgactct tttccactgg taaatggcgg attaatgccg
    20581 aattgcagaa aaacacgttt tttaggttta tcagcatatt gcgctttcaa ttgcacgtac
    20641 tgatccagca gggattgcgc ggcttgttcg gccttgtctg gttgcggact ccagggggcc
    20701 agttgacgta acgcattggc aatttgttca atgcttgtcg catcgaccca catcactttt
    20761 attcccagcg aagccagctg gtcaacctgc cgctcggcat tacctccacg ccaggcaatc
    20821 accagatcgg gtttcagcgc gacaatgcgt tccagattca tcccctgcca ggtggaaacc
    20881 tgctcaatct tttgcgcttg tggaggatag tcggaatagc tgctgacccc aaccggcgtg
    20941 atcccggcgg caaaggcaag ttcagtgttg gcgggagaaa gcgtgatgac gcgcggcgcg
    21001 gcgttgagcc acagtggcgc gagaaaagac agggcgacca gcgccctgaa cagtgattta
    21061 gccatgtgcc agtttctgca ccagtgactc aaccatcagg ctggactgtt tagcggcaac
    21121 agccaggaac tcatcgaagc taagatgaga ctgttgatcg gccacgtcgg agatggcgcg
    21181 tacgacaaca aacgggacgt tgaaattgtg gcagacatgg gcgattgccg tcgcttccat
    21241 ctctacagca atggcctgtg ggaagttgtg gcggattttc gccagaccaa cagaaccgtt
    21301 gatgaaagcg tcgccgctaa caatcaggcc acgtacagcg ttaagattca gttcggcaat
    21361 gcaggcctca gcggcagcga tcagtttatc gtcagcttta aagcctgccg gacagcctgg
    21421 taactgaccg tattcataac caaatgccgt gacatccgcg tcgtgataac gcgcttcgtc
    21481 cgagacaacg atatcgccca ctttcagcgt tggtgccagg ccaccggcag aaccggtgtt
    21541 aataatcaca tctggcttgc agtgttccaa cagcagagtg gcacccagcg ccgcagcgac
    21601 tttaccgatg cccgatttca gaagcgcaac ctcggttcca ttcagttggc cggtatagat
    21661 ttcgcaaccg ccgagactga tagtttgacg gttttcgatt ttgtcacgca gcagcgtaac
    21721 ttcttcttcc attgcaccaa tgatgccgat tttcatagat ttactcgcga taagcccgat
    21781 ttgaaggcat agtttaccat gcgcttacgg ggaagcgtat ttctcacgcg ggagaggaca
    21841 tggcacagat tgatttccga aaaaaaataa actggcatcg tcgttaccgt tcaccgcagg
    21901 gcgttaaaac cgaacatgag atcctgcgga tcttcgagag cgatcgcggg cgtatcatca
    21961 actctccggc aattcgtcgt ctgcaacaaa agacccaggt ttttccactg gagcgcaatg
    22021 ccgccgtgcg cacgcgtctt acccactcga tggaagtcca gcaggtggga cgctacatcg
    22081 ccaaagaaat tttaagccgt ctgaaagagc ttaaattgct ggaagcatac ggcctggatg
    22141 aactgaccgg cccctttgaa agcattgttg agatgtcatg cctgatgcac gatatcggca
    22201 atccgccgtt tggtcatttt ggcgaagcag cgataaatga ctggtttcgc caacgtttgc
    22261 acccggaaga tgccgaaagc cagcctctga ctgacgatcg ctgcagcgtg gcggcactac
    22321 gtttacggga cggggaagaa ccgcttaacg agctgcggcg caagattcgt caggacttat
    22381 gtcattttga ggggaatgca caaggcattc gtctggtgca tacattgatg cggatgaatc
    22441 tcacttgggc acaggttggc ggtattttaa aatatacccg tccggcgtgg tggcgtggcg
    22501 aaacgcctga gacacatcac tatttaatga aaaagccggg ttattatctt tctgaagaag
    22561 cctatattgc ccgcttgcgt aaagaactta atttggcgct ttacagtcgt tttccattaa
    22621 cgtggattat ggaagctgcc gacgacatct cctattgtgt ggcagacctt gaagatgcgg
    22681 tagagaaaag aatatttacc gttgagcagc tttatcatca tttgcacgaa gcgtggggcc
    22741 agcatgagaa aggttcgctc ttttcgctgg tggttgaaaa tgcctgggaa aaatcacgct
    22801 caaatagttt aagccgcagt acggaagatc agttttttat gtatttacgg gtaaacaccc
    22861 taaataaact ggtaccctac gcggcacaac gatttattga taatctgcct gcgattttcg
    22921 ccggaacgtt taatcatgca ttattggaag atgccagcga atgcagcgat cttcttaagc
    22981 tatataaaaa tgtcgctgta aaacatgtgt ttagccatcc ggatgtcgag cagcttgaat
    23041 tgcagggcta tcgggtcatt agcggattat tagagattta tcgtccttta ttaagcctgt
    23101 cgttatcaga ctttactgaa ctggtagaaa aagaacgggt gaaacgtttc cctattgagt
    23161 cgcgcttatt ccacaaactc tcgacgcgcc atcggctggc ctatgtcgag gctgtcagta
    23221 aattaccgtc agattctcct gagtttccgc tatgggaata ttattaccgt tgccgcctgc
    23281 tgcaggatta tatcagcggt atgaccgacc tctatgcgtg ggatgaatac cgacgtctga
    23341 tggccgtaga acaataacca gtcttttgta aagacgaaca ataaattttt accttttgca
    23401 gaaactttag ttcggaactt cgggctataa aacgaatctg aagaacacag caattttgcg
    23461 ttatctgtta atcgagactg aaatacatga aaaaaaacac attagcactg agtgcactgg
    23521 ctctgagttt aggtttggcg ttatctccgc tctctgcaac ggcggctgag acttcttcag
    23581 caacgacagc ccagcagatg ccaagccttg caccgatgct cgaaaaggtg atgccttcag
    23641 tggtcagcat taacgtagaa ggtagcacaa ccgttaatac gccgcgtatg ccgcgtaatt
    23701 tccagcagtt cttcggtgat gattctccgt tctgccagga aggttctccg ttccagagct
    23761 ctccgttctg ccaggggggc cagggcggta atggcggcgg ccagcaacag aaattcatgg
    23821 cgctgggttc cggcgtcatc attgatgccg ataaaggcta tgtcgtcacc aacaaccacg
    23881 ttgttgataa cgcgacggtg attaaagtcc agctgagcga tggtcgtaag ttcgacgcga
    23941 agatggttgg caaagatccg cgctctgata tcgcgctgat ccaaatccag aacccgaaaa
    24001 aactgaccgc aattaagatg gcggattctg atgcactgcg cgtgggtgat tacaccgtag
    24061 cgattggtaa cccgtttggt ctgggcgaga cggtaacttc cgggattgtc tctgcgctgg
    24121 ggcgtagcgg cctgaatgct gaaaactacg aaaacttcat ccagaccgat gcagcgatca
    24181 accgtggtaa ctccggtggt gcgctggtta acctgaacgg cgaactgatc ggtatcaaca
    24241 ccgcgatcct cgcaccggac ggcggcaaca tcggtatcgg ttttgctatc ccgagtaaca
    24301 tggtgaaaaa cctgacctcg cagatggtgg aatacggcca ggtgaaacgc ggtgagctgg
    24361 gtattatggg gactgagctg aattccgaac tggcgaaagc gatgaaagtt gacgcccagc
    24421 gcggtgcttt cgtaagccag gttctgccta attcctccgc tgcaaaagcg ggcattaaag
    24481 cgggtgatgt gatcacctca ctgaacggta agccgatcag cagctttgcc gcactgcgtg
    24541 ctcaggtggg tactatgccg gtgggcagca aactgaccct gggcttactg cgcgacggta
    24601 agcaggttaa cgtgaacctg gaactgcagc agagcagcca gaatcaggtt gattccagct
    24661 ccatcttcaa cggcattgaa ggcgctgaga tgagcaacaa aggcaaagat cagggcgtgg
    24721 tagtgaacaa cgtgaagacg ggcactccgg ctgcgcagat cggcctgaag aaaggtgatg
    24781 tgattattgg tgcgaaccag caggcagtga aaaacatcgc tgaactgcgt aaagttctcg
    24841 acagcaaacc gtctgtgctg gcactgaaca ttcagcgcgg cgacagcacc atctacctgt
    24901 taatgcagta atctccctca accccttcct gaatacggga aggggttctc cttacaatct
    24961 gtgaacttcg ccacaactcc atacatcttc atcatccttt gggcatttgc acaatgccgt
    25021 acgttacgta cttccttatg ctaagccgtg cataacggag gacttatggc tggctggcat
    25081 cttgatacca aaatggcgca ggatatcgtg gcacgtacca tgcgcatcat cgataccaat
    25141 atcaacgtaa tggatgcccg cgggcgaatt atcggcagcg gcgatcgtga gcgtattggt
    25201 gaattgcacg aaggtgcatt gctggtactt tcacagggac gagtcgtcga tatcgatgac
    25261 gcggtagcac gtcatctgca cggtgtgcgg caggggatta atctaccgtt acggctggaa
    25321 ggtgaaattg tcggcgtaat tggcctgaca ggtgaaccag agaatctgcg taaatatggc
    25381 gaactggtct gcatgacggc tgaaatgatg ctggaacagt cgcggttgat gcacttgttg
    25441 gcgcaggata gccgtttgcg ggaagaactg gtgatgaacc tgattcaggc agaggagaat
    25501 actcccgcac ttctgaatgg gcgcaacggc tggggatcga tctcaatcaa ccgcgagtgg
    25561 tggctattgt tgaggtcgac agcggtcagc ttggcgtgga cagcgcaatg gcggagttac
    25621 aacaactgca aaacgcgctg actacgcccg agcgtaataa tctggtggcg attgtctcgc
    25681 taaccgaaat ggtggtgttg aaaccggcgt tgaactcttt tgggcgctgg gatgcagaag
    25741 atcatcgtaa gcgagttgaa caactgatta cccgcatgaa agagtacggc cagctgcgtt
    25801 ttcgcgtttc actgggcaac tattttaccg gtcctggcag tattgcccga tcctatcgta
    25861 cggcgaaaac gacgatggtg gtgggtaaac agcggatgcc agaaagtcgc tgctattttt
    25921 atcaggatct gatgttacct gtgttactcg acagtttgcg tggcgactgg caggccaacg
    25981 aactggcgcg accgctggcg cggctgaaag cgatggacaa taacggcttg ctgcgacgaa
    26041 cgctggcggc gtggtttcgc cacaatgtgc aaccgctggc aacgtcaaag gcgttgttta
    26101 ttcatcgtaa taccctggag tatcggctta atcgtatatc ggaactgacc gggcttgatt
    26161 tgggcaattt tgatgacagg ttgctgctgt atgtggcgtt acaactggat gaagagcggt
    26221 agattatgcg ttaaggttga ggcggcgctg gctcattccc ctcagcccga aggggagagg
    26281 ggaatgcccg agttaaccgt caattcggga ttatttacgc gttagttttt ccagatcggc
    26341 ttcaatctcg ctgatcttat tggttacgac tgactccagg tgacgtaagt cgtcgaggat
    26401 cttacgctta agatcaactt cgctgcggtc acgctgacag atttgatcaa gctcatcaat
    26461 gatataccgt agattcgggc tgatttcctg gacttcttta taaccctgac ccacaccatc
    26521 agcgacgacc gttttacgct gacgcggata tttaaactta acgctcttgg cgaaaaactc
    26581 gcctttgtct ttctggaaat agattttcag aatatcgttg ttggcttcct gccggaggct
    26641 gtaacgatca atttcttcag gattggtaat acccagactt ttcagattgt cgtacatagc
    26701 gttacctcaa aatgagtcag taaattgtgc ttattttagc atttggcttg gcccgcccgg
    26761 ctcgactgtg atcgcagaaa gcctttttct tccagacttc tatcatggcg taaaaatcaa
    26821 aaaattacct gctttattgt ggcgatattc catgggatgg attagcacat ttatcttcgt
    26881 tttgcgaatt acatgacagt ccgaaacaag atgaaaaagt atttaaaaaa gggggaggaa
    26941 gaatgagtag gacgaattat tctgagccag aaagcatacg ggcagcatca tgctgcccgt
    27001 atatgtactc ttaatcgatg gtacgcagca gttcgttaat gccgactttg ccgcgagttt
    27061 tcgcgtcaac tttcttaacg ataaccgcac agtagaggct gtatttgcca tcttttgacg
    27121 gcagattacc tgaaacaacc acagaccccg ccggaacgcg accgtagtgg atttcgccgg
    27181 tttcacggtc gtaaatacgg gtgctctgac caatgtatac gcccatagag atgacggaac
    27241 cttcttcgac aatcacccct tcaaccactt cagagcgtgc gccaatgaag cagttatctt
    27301 caataatggt cgggttagcc tgcagcggct ccagcacgcc gccgatgccc acgccaccgg
    27361 aaaggtggac gtttttacca atctgcgcac aagaaccgac ggtcgcccag gtatcaacca
    27421 tggtgccttc atcaacatat gcgccgatgt taacgtaaga cggcatcagc acggtgttac
    27481 gggcaataaa cgcaccctga cgtaccgccg ctggtggcac aacgcggaag ccttctttct
    27541 ggaaacgtgc ttcgtcgtag tcggcgaatt tcatcggcac tttgtcgaag tagcggcttt
    27601 ctgccccttc gatcacctga ttatcattaa tacggaaaga gagcagcacc gcttttttca
    27661 accactgatg cgtcacccac tgaccgtcaa ttttttccgc tacacgcagt gcgccggaat
    27721 ccagcagggc gatcacctga tttaccgctt cgcgggtaac ggtgtctgca ttggctggcg
    27781 tgatctcggc acggcgttca aaagcggttt caataatgtt ctgtaactgc tgcattgtta
    27841 aactcttttc atatcagtaa acacatcacc ctttatcgtt tggattgagg gcctctgtca
    27901 accgctgatg cacttcctgc tgcaactcgt tattaagcgc acgccggtca gcggtggcaa
    27961 ttatgaataa atcttctact cgctcgccaa tggttgtaat tcgggcacca tgaagcgaaa
    28021 ttcccagatc ggcaaaaatt ttcccgactc gcgccagcag tccaggttgg tcgagggcga
    28081 tcagttcgag gaacgatttg cggtcggtat gggtcggcaa aaacgttact tcggtttcaa
    28141 cagtaaaatg gcgtaatttg gcgggttggc gacggggctg cggtggctgc cagctacttt
    28201 gcgtcagcac ctgctccaga ccaaaccgaa taacctcatg gcgatctgcg gacagcgggc
    28261 tgccatcggg ttccagcacg ataaaggtat ccatcgccat accgtcgcga gtggtgaaaa
    28321 tttgtgcgtc gtgaacactt aaattgcggc ggtctaattc ggcacagacg gcggcaaaca
    28381 gataggggcg gtccgggctc cagataaaaa tctcggtgcc tccacgcgta gcctgcgggc
    28441 taagcaatac cagcggtttg cttaaatcat gctgtaataa atggcgggca tgccaggcca
    28501 gttgatttgg gctatggcgg acaaaatagt tagcacgaca gcgtgaccaa atttggtgca
    28561 gcgcctcttc gtcgatgtta tccatgcgca gtagtgccag tgcctggagt tggtgatggc
    28621 gaacccgttc gcgcatatcc ggcgtgtttt gcattccgcg tcgtagctgc ttttcggtgg
    28681 caaaatagag ttcgcgcaac agactttgct tccagctatt ccacagtgtt tcgttggtgg
    28741 cgcaaatgtc agccactgtc aggcatacca gatagcgcag acgattttcc gtttgaactt
    28801 cttcggcaaa ctgcttgatg acttcagggt cctgaatatc gcggcgttgt gctgtcaccg
    28861 acatcagcaa gtgctggcga accagccagg caaccagctg tgtttcgcgt gagttcagcc
    28921 cgtggagttc tgcaaaatgc actacatcct gagcaccgag aatggagtgg tcgccgccgc
    28981 gtcctttggc gatatcgtga aacagcgcgg cgatgaaaat cagctcagtt gacggcaggc
    29041 gcggccagac gtccacacac aacggatggc gctggcgcgt ttcttcactg gcaaaactct
    29101 ccagtttcag catcacgcgg atagtatgtt catccaccgt gtaggcgtgg aacagatcaa
    29161 actgcatctg cccgacgata tgcgaccatt gcggcatata cgcgccgagc acgctatggc
    29221 gatgcattgg caatagtccg cgccgcaccg ctccggggtg acgcagaatg ctcaaaaaca
    29281 gttttcgtgc ttgcggaata ttacacagcg gttgttgcag atggcgacgg gcatggcgta
    29341 actggcgcag cgtggtggag taaatgccgg tgatcgcact gttgcgcacc atggtgtaga
    29401 acatacgcaa gatggcttcc ggctggcgca taaatagtgt ttcatcacgc aggtcgatta
    29461 gcgtaccgcg tagctgaaac tcatcgtcga gtggacgtgg tttttcgtcg gcgggaaggg
    29521 cgaggatggc ttcatcgaac agttgcagca gcatctggtt gagttcactg acgcggcgtg
    29581 taacgcggaa gtaatccttc atcatccgct cgaccggttc gttgccttca ccactgtaat
    29641 tcagacgctg agcgacgcta agttggcgat cgaataacag acgattatcg taacggctga
    29701 cgaccagatg cagggcaaag cgaatacgcc acagtatatg cagacattcg tttaattccg
    29761 cccgctccgc tgaggttaag aagccaaacc cgaccatttc atccagcgat gttgcgccaa
    29821 aatgacggcg ggccacccat tgcagagtgt gaatatcgcg caagccgcca gggctacttt
    29881 tgatgtctgg ttcaaggttg tagctggtgc catggtaacg ctgatggcgc tggttctgtt
    29941 cttcaacttt cgctgcgtag aacttgtcgg aaggccagaa tccttcgcta aaaatatgtt
    30001 tttgcagttc gaggaagagc gcaacatcgc caattaataa gcgggattcg attaaattgg
    30061 tggcgacggt taaatccgat aacccttcca acatgcactc ttcaagcgtg cgcacactat
    30121 gaccgacttc cagctttaca tcccagagca gcgttaacag ctcgcccact ttttgcgcct
    30181 gatcgtccgg gagcttttta cggcttaaaa tcagtaaatc gatgtcagaa agtggatgca
    30241 gctcgccacg accgtagcca ccgacggcaa ccaatgccag gtcggcaatc tggctgaatc
    30301 ccgcttcaat ccataatcgt tgcaggagct ggtctatgaa ctcggtacgt gcctcaatca
    30361 actgttctgc agagatccca ttgtcaaagg catcacccag ccaacgctgg aaagtatcga
    30421 tatgggcttt tatcccacag acggttaatt catcacgggg ccaggcgcat ggattttgcg
    30481 gttgaccggg cagggtggag agagcggtgt ttgcgtactg ttctggaagg gtattcattg
    30541 tgcgccaccc ataagattaa attatcgcat taaaaaagcc ggcattcgcc ggcttcatct
    30601 tattcgtcgt gcgagattat cgccgggatg gtgtcatcct tgcgtagcgt caaaatttcg
    30661 cagccgttat cagtcaccac aatagtatgc tcatattgtg cagacaagct gcgatctttg
    30721 gtttttaccg tccagccatc tttcatggtg cggatctctt ttttacccgc gttgaccatt
    30781 ggctcaatgg tgaacgtcat ccccggtttc agcacgacgt tggtttcacg ggagtcatag
    30841 tgcagcacct gcggttcttc atggaagccg cgaccaatac cgtgtccgca atattcacga
    30901 acgacggaga agccttctgc ttcgacaaat ttctgaatcg ccgcaccgat ttcgcgcaga
    30961 ttaatgcctg gttttaccat gcgtagcgcc aggtacaggc tttcttgcgt gatgcggcac
    31021 aggcgttcgc ccatgatggt cggcttaccg acgataaaca ttttcgaggt atcgccgtgg
    31081 aaaccatctt tgattacggt gacatcaatg ttaacgatat cgccatcttt cagcagctta
    31141 gcatcgtccg ggataccgtg gcacaccact tcattaatag agatgcaaac ggatttcgga
    31201 tagccgtgat agccgaggca ggcagaaacc gcgtgttgtt cattaacaat gtaatcatta
    31261 cagatgcgat ccagctcgcc ggtgctgacg cccggtttaa catacggttc gatcatctcc
    31321 agcacttcgg cagccagtcg gccagcgacg cgcatttttt cgatatcttc tggggtcttg
    31381 attgagatag ccattaattc tgtccatcag cgtcggtgat accgacaata tatatgtaag
    31441 tgccgtcaat ggtatcacac ccgggcaaat tgagaatcat tctgaatttc gccaaacgtg
    31501 ccactgaagg ttttctataa tagaaaattc gacgtctgat gctgtacaca gcgccaacaa
    31561 ttattggtgt ccacgacgta tttgtggtat aaagcgcgcc ggacttccga tccatttcgt
    31621 atacacagac tggacggaag cgacaatctc actttgtgta acaacacaca cgtatcggca
    31681 catattccgg ggtgcccttt ggggtcggta atatgggata cgtggaggca taaccccaac
    31741 ttttatatag aggttttaat catggcaact gtttccatgc gcgacatgct caaggctggt
    31801 gttcacttcg gtcaccagac ccgttactgg aacccgaaaa tgaagccgtt catcttcggt
    31861 gcgcgtaaca aagttcacat catcaacctt gagaaaactg taccgatgtt caacgaagct
    31921 ctggctgaac tgaacaagat tgtttctcgc aaaggtaaaa tccttttcgt tggtactaaa
    31981 cgcgctgcaa gcgaagcggt gaaagacgct gctctgagct gcgaccagtt cttcgtgaac
    32041 catcgctggc tgggcggtat gctgactaac tggaaaaccg ttcgtcagtc catcaaacgt
    32101 ctgaaagacc tggaaactca gtctcaggac ggtactttcg acaagctgac caagaaagaa
    32161 gcgctgatgc gcactcgtga gctggagaaa ctggaaaaca gcctgggcgg tatcaaagac
    32221 atgggcggtc tgccggacgc tctgtttgta atcgatgctg accacgaaca cattgctatc
    32281 aaagaagcaa acaacttggg tattccggta tttgctatcg ttgataccaa ctctgatccg
    32341 gacggtgttg acttcgttat cccgggtaac gacgacgcaa tccgtgctgt gaccctgtac
    32401 ctgggcgctg ttgctgcaac cgtacgtgaa ggccgttctc aggatctggc ttcccaggcg
    32461 gaagaaagct tcgtagaagc tgagtaataa ggcttgataa ctcccccaaa atagttcgag
    32521 ttgcagaaag gcggcaagct cgagaattcc cgggagctta catcagtaag tgaccgggat
    32581 gagcgagcga agacaacgca tctgcggcgc gaaatatgaa gggggagagc ccttatagac
    32641 caggtagtac acgtttggtt agggggcctg catatggccc cctttttcac ttttatatct
    32701 gtgcggttta atgccgggca gatcacatct ccgaggattt tagaatggct gaaattaccg
    32761 catccctggt aaaagagctg cgtgagcgta ctggcgcagg catgatggat tgcaaaaaag
    32821 cactgactga agctaacggc gacatcgagc tggcaatcga aaacatgcgt aagtccggtg
    32881 ctattaaagc agcgaaaaaa gcaggcaacg ttgctgctga cggcgtgatc aaaaccaaaa
    32941 tcgacggcaa ctacggcatc attctggaag ttaactgcca gactgacttc gttgcaaaag
    33001 acgctggttt ccaggcgttc gcagacaaag ttctggacgc agctgttgct ggcaaaatca
    33061 ctgacgttga agttctgaaa gcacagttcg aagaagaacg tgttgcgctg gtagcgaaaa
    33121 ttggtgaaaa catcaacatt cgccgcgttg ctgcgctgga aggcgacgtt ctgggttctt
    33181 atcagcacgg tgcgcgtatc ggcgttctgg ttgctgctaa aggcgctgac gaagagctgg
    33241 ttaaacacat cgctatgcac gttgctgcaa gcaagccaga attcatcaaa ccagaagacg
    33301 tatccgctga agtggtagaa aaagaatacc aggtacagct ggatatcgcg atgcagtctg
    33361 gtaagccgaa agaaatcgca gagaaaatgg ttgaaggccg catgaagaaa ttcaccggcg
    33421 aagtttctct gaccggtcag ccgttcgtta tggaaccaag caaaactgtt ggtcagctgc
    33481 tgaaagagca taacgctgaa gtgactggct tcatccgttt cgaagtgggt gaaggcatcg
    33541 agaaagttga gactgacttt gcagcagaag ttgctgcgat gtccaagcag tcttaattat
    33601 caaaaaggag ccgcctgagg gcggcttctt tttgtgccca tcttgtaaat tcagctaacc
    33661 cttgtggggc tgcgctgaaa agcgacgtac aatgtcgcta gtattaattc atttcaatcg
    33721 ttgacagtct caggaaagaa acatggctac caatgcaaaa cccgtctata aacgcattct
    33781 gcttaagttg agtggcgaag ctctgcaggg cactgaaggc ttcggtattg atgcaagcat
    33841 actggatcgt atggctcagg aaatcaaaga actggttgaa ctgggtattc aggttggtgt
    33901 ggtgattggt gggggtaacc tgttccgtgg cgctggtctg gcgaaagcgg gtatgaaccg
    33961 cgttgtgggc gaccacatgg ggatgctggc gaccgtaatg aacggcttgg caatgcgtga
    34021 tgcactgcac cgcgcctatg tgaacgctcg tctgatgtcc gctattccat tgaatggcgt
    34081 gtgcgacagc tacagctggg cagaagctat cagcctgttg cgcaacaacc gtgtggtgat
    34141 cctctccgcc ggtacaggta acccgttctt taccaccgac tcagcagctt gcctgcgtgg
    34201 tatcgaaatt gaagccgatg tggtgctgaa agcaaccaaa gttgacggcg tgtttaccgc
    34261 tgatccggcg aaagatccaa ccgcaaccat gtacgagcaa ctgacttaca gcgaagtgct
    34321 ggaaaaagag ctgaaagtca tggacctggc ggccttcacg ctggctcgtg accataaatt
    34381 accgattcgt gttttcaata tgaacaaacc gggtgcgctg cgccgtgtgg taatgggtga
    34441 aaaagaaggg actttaatca cggaataatt cccgtgatgg ataaataagg gtaagattcc
    34501 gcgtaagtat cgcgggggcg taagtctggt tataaggcgt tattgttgca ggcagtttgg
    34561 tcacggccag cgcgcagcaa ccggagcgta caaaagtacg tgaggatggc gagcactgct
    34621 cggggccaaa atggcaaata aaatagccta ataatccaga cgattacccg taatatgttt
    34681 aatcagggct atacttagca cacttccact gtgtgtgact gtctggtctg actgagacaa
    34741 gttttcaagg attcgtaacg tgattagcga tatcagaaaa gatgctgaag tacgcatgga
    34801 caaatgcgta gaagcgttca aaacccaaat cagcaaaata cgcacgggtc gtgcttctcc
    34861 cagcctgctg gatggcattg tcgtggaata ttacggcacg ccgacgccgc tgcgtcagct
    34921 ggcaagcgta acggtagaag attcccgtac tctgaaaatc aacgtgtttg atcgttcaat
    34981 gtctccggcc gttgaaaaag cgattatggc ctccgatctc ggcctgaacc cgaactctgc
    35041 gggtagcgac atccgtgttc cgctgccgcc gctgacggaa gaacgtcgta aagatctgac
    35101 caaaatcgtt cgtggtgaag cagaacaagc gcgtgttgca gtacgtaatg tgcgtcgtga
    35161 cgcgaacgac aaagtgaaag cactgttgaa agataaagag atcagcgaag acgacgatcg
    35221 ccgttctcag gacgatgtac agaaactgac tgatgctgca atcaagaaaa ttgaagcggc
    35281 gctggcagac aaagaagcag aactgatgca gttctgattt cttgaacgac aaaaacgccg
    35341 ctcagtagat ccttgcggat cggctggcgg cgttttgctt tttattctgt ctcaactctg
    35401 gatgtttcat gaagcaactc accattctgg gctcgaccgg ctcgattggt tgcagcacgc
    35461 tggacgtggt gcgccataat cccgaacact tccgcgtagt tgcgctggtg gcaggcaaaa
    35521 atgtcactcg catggtagaa cagtgcctgg aattctctcc ccgctatgcc gtaatggacg
    35581 atgaagcgag tgcgaaactt cttaaaacga tgctacagca acaggggagc cgcaccgaag
    35641 tcttaagtgg gcaacaagcc gcttgcgata tggcagcgct tgaggaggtt gatcaggtga
    35701 tggcagccat tgttggtgct gctgggctgt tacctacgct tgctgcgatc cgcgcgggta
    35761 aaaccatttt gctggccaat aaagaatcac tggttacctg cggacgtctg tttatggacg
    35821 ccgtaaagca gagcaaagcg caattgttac cggtcgatag cgaacataac gctatttttc
    35881 agagtttacc gcaacctatc cagcataatc tgggatacgc tgaccttgag caaaatggcg
    35941 tggtgtccat tttacttacc gggtctggtg gccctttccg tgagacgcca ttgcgcgatt
    36001 tggcaacaat gacgcccgat caagcttgcc gtcatccgaa ctggtcgatg ggacgtaaaa
    36061 tttctgtcga ttcggctacc atgatgaaca aaggtctgga atacattgaa gcgcgttggc
    36121 tgtttaacgc cagcgccagc cagatggaag tgctgattca cccgcagtca gtgattcact
    36181 caatggtgcg ctatcaggac ggcagtgttc tggcgcagct gggggaaccg gatatgcgta
    36241 cgccaattgc ccacaccatg gcatggccga atcgcgtgaa ctctggcgtg aagccgctcg
    36301 atttttgcaa actaagtgcg ttgacatttg ccgcaccgga ttatgagcgt tatccatgcc
    36361 tgaaactggc gatggaggcg ttcgaacaag gccaggcagc gacgacagca ttgaatgccg
    36421 caaacgaaat caccgttgct gcttttcttg cgcaacaaat ccgctttacg gatatcgctg
    36481 cgttgaattt atccgtactg gaaaaaatgg atatgcgcga accacaatgt gtggacgatg
    36541 tgttatctgt tgatgcgaac gcgagtgaag tcgccagaaa agaggtgatg cgtctcgcaa
    36601 gctgaggata atccggctac agagagtcgc gctatttgtt agcgtagggc ttcagtgata
    36661 tagtctgcgc catctgatcg taagtagttg gctttataag gtcaggtatg ccgtggtttt
    36721 acacggcttt tttttgtata ggcttcagta ttcctgagta ccgtaaaccc tgtcagggaa
    36781 taaaaaacgc gtgatgttgt ctgctactca accacttagc gaaaaattgc cagcgcatgg
    36841 ctgccgtcat gttgcgatca ttatggacgg caatggtcgc tgggcaaaaa agcaagggaa
    36901 gattcgtgcc tttgggcata aagccggggc aaaatccgtc cgccgggctg tctcttttgc
    36961 ggccaacaac ggtattgagg cgttaacgct gtatgccttt agtagtgaaa actggaaccg
    37021 accagcgcag gaagtcagtg cgttaatgga actgtttgtg tgggcgctcg atagcgaagt
    37081 aaaaagtctg caccgacata acgtgcgtct gcgtattatt ggcgatacca gtcgttttaa
    37141 ctcgcgtttg caagaacgta ttcgtaaatc tgaagcgcta acagccggaa ataccggtct
    37201 gacgctgaat attgcggcga actacggtgg acgttgggat atagtccagg gagtcaggca
    37261 actggctgaa aaggtgcagc aaggaaacct gcaaccagat cagatagatg aagagatgtt
    37321 aaaccagcat gtctgtatgc atgaactggc ccctgtagat ttagtaatta ggactggggg
    37381 agagcatcgc attagtaact ttttgctttg gcaaattgcc tatgccgaac tttactttac
    37441 agatgttctc tggcccgatt tcgatgaaca agactttgaa ggggcgttaa atgcctttgc
    37501 taatcgagag cgtcgtttcg gcggcaccga gcccggtgat gaaacagcct gatgggggtc
    37561 gcttttgctg aagtatcgcc tgatatctgc ttttgtgtta atacccgtcg tcatcgcggc
    37621 gttgtttctg ttgccgccgg tggggttcgc cattgtaacg ctggtggtct gcatgctggc
    37681 agcgtgggaa tggggacagc ttagcggttt taccactcgt tcgcagcgag tatggttggc
    37741 ggtgttatgc gggttattgt tggcgctgat gctttttctg ttgccggaat atcaccgaaa
    37801 tattcatcaa ccgctggttg aaatctcact ttgggcttcg ttgggttggt ggattgtcgc
    37861 gctattgctg gtgctgtttt acccaggttc cgcagcaatc tggcgtaact ctaaaacatt
    37921 gcgccttatt tttggcgtgc taaccattgt tcccttcttc tggggcatgc tggcgttacg
    37981 ggcctggcac tatgacgaga atcattacag tggcgcaata tggctgctct atgtcatgat
    38041 cctggtatgg ggcgctgact ccggcgcata tatgtttggc aaattgtttg gtaaacataa
    38101 gctggcaccg aaggtttctc cgggtaaaac ctggcaaggc tttataggtg gactcgctac
    38161 tgcagcggta atctcatggg gttatggcat gtgggcgaat ctcgacgtcg ctcccgtcac
    38221 cttactcatt tgctctattg tcgcagcgtt agcctcagtg ctcggcgatc tgaccgagag
    38281 tatgtttaag cgtgaagcag gaattaagga cagcggtcat ttaattccag gacacggtgg
    38341 tattttagat cgtattgata gcctgacggc tgcggtaccg gtctttgctt gcttgttgtt
    38401 actggtattc aggacgcttt aacggaaggt aatatgctga gttttctctg ggatttggct
    38461 tcgttcatcg ttgcactggg tgtacttatc accgtgcatg aatttggtca tttctgggtt
    38521 gcccggcgtt gtggtgttcg cgttgagcgt ttctcaatag ggtttggtaa ggcgctctgg
    38581 cggcgaactg ataagctcgg caccgaatat gttatcgccc tgatcccgtt gggcggttat
    38641 gtcaaaatgc tggatgagcg cgcagaaccg gtcgttccgg aactccgcca ccatgccttc
    38701 aataataaat ctgtcggcca acgagcggcg attattgccg caggtccggt tgcaaacttc
    38761 atttttgcta tctttgccta ctggctggtt tttattattg gtgtgcctgg cgtacgtccg
    38821 gtggttggcg aaatagcagc caattcgata gctgcggaag cacaaattgc accaggtacg
    38881 gaactaaaag ccgtagatgg tatcgaaacg cctgattggg atgccgtgcg tttgcagttg
    38941 gtcgataaaa ttggcgatga aagcaccacc attacagtag cgccatttga cagcgaccaa
    39001 cggcgggatg taaagctcga tttacgtcac tgggcgtttg agcctgataa agaagatccg
    39061 gtatcttcgc tggggattcg tcctcgtggg ccgcaaattg aacctgtact ggaaaatgtg
    39121 cagccaaact cggcggcaag caaggcaggt ttgcaagcag gcgacaggat cgttaaagtc
    39181 gatggtcagc ccttaacgca gtgggtgacc tttgtgatgc ttgtccggga taacccgggt
    39241 aaatccttag cgttagaaat cgaaaggcag gggagtccct tgtctttgac attaatcccg
    39301 gagagtaaac cgggtaatgg taaagcgatt ggttttgtcg gtattgagcc gaaagtcatt
    39361 cctttgccag atgagtataa agttgtacgc cagtatgggc cgttcaacgc catcgtcgaa
    39421 gccacggaca aaacgtggca gctgatgaag ctgacggtca gtatgctggg aaaattgatc
    39481 accggtgatg tgaaactgaa caacctcagt gggccgatct ctatcgccaa gggggctggg
    39541 atgacagcgg aactcggggt agtttattac ctgccgtttc ttgcgcttat tagcgtgaac
    39601 ttagggataa ttaacctgtt tccgttgccc gtacttgacg gggggcatct gctgttcctt
    39661 gcgatcgaaa agatcaaggg cggaccggta tccgagcggg ttcaagactt ttgttatcgc
    39721 attggctcga ttctgctggt gctgttaatg gggcttgcac ttttcaatga tttctctcgg
    39781 ttatgagagt tagttaggaa gaacgcataa taacgatggc gatgaaaaag ttgctcatag
    39841 cgtcgctgct gtttagcagc gccaccgtat acggtgctga agggttcgta gtgaaagata
    39901 ttcatttcga aggccttcag cgtgtcgccg ttggtgcggc cctcctcagt atgccggtgc
    39961 gtacaggcga cacggttaat gatgaagata tcagtaatac cattcgcgct ctgtttgcta
    40021 ccggcaactt tgaggatgtt cgcgtccttc gtgatggtga tacccttctg gttcaggtaa
    40081 aagaacgtcc gaccattgcc agcattactt tctccggtaa caaatcggtg aaagatgaca
    40141 tgctgaagca aaacctcgag gcttctggtg tgcgtgtggg cgaatccctc gatcgcacca
    40201 ccattgccga tatcgagaaa ggtctggaag acttctacta cagcgtcggt aaatatagcg
    40261 ccagcgttaa agctgtcgtg accccgctgc cgcgcaaccg tgttgaccta aaactggtgt
    40321 tccaggaagg tgtgtcagct gaaatccagc aaattaacat tgttggtaac catgctttca
    40381 ccaccgacga actgatctct catttccaac tgcgtgacga agtgccgtgg tggaatgtgg
    40441 taggcgatcg taaataccag aaacagaaac tggcgggcga tcttgaaacc ctgcgcagct
    40501 actatctgga ccgcggttat gcccgtttca acatcgactc tacccaggtc agtctgacgc
    40561 cagataaaaa aggtatttac gtcacggtga acatcaccga aggcgatcag tacaagcttt
    40621 ctggcgttga agtgagcggc aaccttgccg ggcactccgc tgaaattgag caactgacta
    40681 agatcgagcc gggcgagctg tataacggca ctaaagtgac caaaatggaa gatgacatta
    40741 aaaagcttct cggtcgctat ggttatgcct atccgcgcgt acagtcgatg cctgaaatta
    40801 acgatgccga caaaaccgtt aaattacgcg tgaacgttga tgcgggtaac cgtttctacg
    40861 tgcgtaagat ccgttttgaa ggtaacgata cctcgaaaga tgccgtcctg cgtcgcgaaa
    40921 tgcgtcagat ggaaggtgca tggttgggga gcgatctggt cgatcagggt aaggagcgtc
    40981 tgaatcgtct gggcttcttt gaaactgtcg ataccgatac ccaacgtgtt ccgggtagcc
    41041 cggaccaggt tgatgtagtc tacaaggtga aagaacgtaa cactggtagc ttcaacttcg
    41101 ggattggtta cggtactgaa agtggcgtga gcttccaggc tggcgtgcaa caggataact
    41161 ggttaggtac aggttatgct gttggtatca acgggaccaa aaacgattac cagacctatg
    41221 ctgaactgtc ggtaaccaac ccgtacttca ccgtagatgg cgtaagcctc ggtggtcgtc
    41281 tcttctataa tgacttccag gcagatgacg ccgacctgtc cgactatacc aacaagagtt
    41341 atggtacaga cgtgacgttg ggcttcccga ttaacgaata taactcgctg cgtgcaggtc
    41401 tgggttatgt acataactcc ctgtccaaca tgcagcctca ggttgcgatg tggcgttatc
    41461 tgtactctat gggtgaacat ccgagcacct ctgatcagga taacagcttc aaaacggacg
    41521 acttcacgtt caactatggt tggacctata acaagcttga ccgtggttac ttcccgacag
    41581 atggttcacg tgtcaacctg accggtaaag tgaccattcc tggatcggat aacgaatact
    41641 acaaagtgac gttagacacg gcgacttatg tgccgatcga tgacgatcac aaatgggttg
    41701 ttctgggtcg tacccgctgg ggttatggtg atggtttagg cggcaaagag atgccgttct
    41761 acgagaactt ctatgccggt ggttccagca ccgtgcgtgg cttccagtcc aataccattg
    41821 gtccgaaagc agtttacttc ccgcatcagg ccagtaatta tgatccggac tatgattacg
    41881 aatgtgcgac tcaggacggc gcgaaagacc tgtgtaaatc ggatgatgct gtaggcggta
    41941 acgccatggc ggttgccagc ctcgagttca tcaccccgac gccgtttatt agcgataagt
    42001 atgctaactc ggttcgtact tcattcttct gggatatggg taccgtttgg gatacaaact
    42061 gggattccag ccaatattct ggatatccgg actatagtga tccaagcaat atccgtatgt
    42121 ctgcgggtat cgcattacaa tggatgtccc cattggggcc gttggtgttc tcctacgccc
    42181 agccgttcaa aaagtacgat ggagacaagg cagaacagtt ccagtttaac atcggtaaaa
    42241 cctggtaagt gttctccaca aaggaatgta gtggtagtgt agcgatgact ttaggcgatc
    42301 aatataagat cgccgggcca cgcaaagaac tgcaccctcc ggtgcaaatg ggatggtaag
    42361 gagtttattg tgaaaaagtg gttattagct gcaggtcttg gtttagcact ggcaacttct
    42421 gctcaggcgg ctgacaaaat tgcaatcgtc aatatgggca gcctgttcca gcaggtagcg
    42481 cagaaaaccg gtgtttctaa cacgctggaa aatgagttca aaggccgtgc cagcgaactg
    42541 cagcgtatgg aaaccgatct gcaggctaaa atgaaaaaac tgcagtccat gaaagcgggc
    42601 agcgatcgca ccaaactgga aaaagacgtg atggctcagc gccagacttt tgctcagaaa
    42661 gcgcaggctt ttgagcagga tcgcgcacgt cgttccaacg aagaacgcgg caaactggtt
    42721 actcgtatcc agactgctgt gaaatccgtt gccaacagcc aggatatcga tctggttgtt
    42781 gatgcaaacg ccgttgctta caacagcagc gatgtaaaag acatcactgc cgacgtactg
    42841 aaacaggtta aataagtaat gccttcaatt cgactggctg atttagcgca gcagttggat
    42901 gcagaactac acggtgatgg cgatatcgtc atcaccggcg ttgcgtccat gcaatctgca
    42961 caaacaggtc acattacgtt catggttaac ccaaaatacc gtgagcattt aggcttgtgc
    43021 caggcgtccg cggttgtcat gacccaggac gatcttcctt tcgcgaaaag tgccgcgctg
    43081 gtagtgaaga atccctacct gacttacgcg cgcatggcgc aaattttaga taccacgccg
    43141 cagcccgcgc agaacattgc acccagtgcg gtgatcgacg cgacggcgaa gctgggtaac
    43201 aacgtatcga ttggcgctaa cgcggtgatt gagtccggcg ttgaactggg cgataacgtg
    43261 attatcggtg ccggttgctt cgtaggtaaa aacagcaaaa tcggtgcagg ttcgcgtctc
    43321 tgggcgaacg taaccattta ccatgagatc cagatcggtc agaattgcct gatccagtcc
    43381 ggaacagtgg taggcgcaga cggctttggt tatgccaacg atcgtggtaa ctgggtgaag
    43441 atcccacaga ttggtcgcgt aattattggc gatcgcgtgg agatcggtgc ctgcacaacc
    43501 atcgatcgcg gcgcgctgga tgacactgtt attggcaatg gcgtgatcat tgataaccag
    43561 tgccagattg cacataacgt cgtgattggc gacaatacgg cggttgccgg tggcgtcatt
    43621 atggcgggca gcctgaaaat tggtcgttac tgcatgatcg gcggagccag cgtaatcaac
    43681 gggcatatgg aaatatgcga caaagtgacg gttacgggca tgggtatggt gatgcgtccc
    43741 atcactgaac caggcgtcta ttcctcaggc attccgctgc aacccaacaa agtctggcgc
    43801 aaaaccgctg cactggtgat gaacattgat gacatgagca agcgtctgaa atcgcttgag
    43861 cgcaaggtta atcaacaaga ctaacgttcc atcttttgtt cgccaaactt tacggcctgt
    43921 ctcattctta cgattgcggc aggccgtgtt attattgtcg tttcttatat tttgacagga
    43981 agagtatctt gactactaac actcatactc tgcagattga agagatttta gaacttctgc
    44041 cgcaccgttt cccgttctta ctggtggatc gcgtgctgga ttttgaagaa ggtcgttttc
    44101 tgcgcgcagt aaaaaatgtc tctgtcaatg agccattctt ccagggccat ttccctggaa
    44161 aaccgatttt cccgggtgtg ctgattctgg aagcaatggc acaggcaaca ggtattctgg
    44221 cgtttaaaag cgtaggaaaa ctggaaccgg gtgagctgta ctacttcgct ggtattgacg
    44281 aagcgcgctt caagcgcccg gtcgtgcctg gcgatcaaat gatcatggaa gtcactttcg
    44341 aaaaaacgcg ccgcggcctg acccgtttta aaggggttgc tctggtcgat ggtaaagtag
    44401 tttgcgaagc aacgatgatg tgtgctcgta gccgggaggc ctgatacgtg attgataaat
    44461 ccgcctttgt gcatccaacc gccattgtgg aagagggcgc gtcaattggc gcgaacgcac
    44521 acattggtcc tttttgtatc gttggacccc atgtcgaaat tggtgagggt accgtactga
    44581 aatctcacgt tgtcgtgaat ggtcatacta aaattggccg cgataatgag atttatcagt
    44641 ttgcctccat cggcgaagtt aaccaggatt tgaaatatgc tggcgaaccg acccgtgtgg
    44701 aaatcggcga tcgtaaccgc attcgcgaaa gcgtcaccat tcatcgtggc acagtccagg
    44761 gcggtggatt gacgaaggtg ggcagcgaca acttactgat gatcaacgcg cacattgcgc
    44821 acgattgtac ggtaggtaac cgctgtattc tcgccaacaa cgcaacgctg gcgggtcacg
    44881 tatcggttga cgacttcgcg atcatcggcg gcatgaccgc agtccatcag ttctgcatca
    44941 ttggtgcgca tgtgatggtt ggcggctgct ccggtgtggc gcaggacgtc cctccttatg
    45001 ttattgcgca gggtaaccac gcaacgccgt tcggtgtcaa tatcgaaggg ctgaagcgcc
    45061 gcggattcag ccgtgaggcg attaccgcta tccgcaatgc gtataagcta atttatcgta
    45121 gcggtaaaac gctcgatgaa gtgaaaccgg aaattgctga actggcggaa acatatccgg
    45181 aagtgaaagc ctttaccgat ttctttgcac gctcaacgcg cggtctgatt cgttaatgac
    45241 tgaccagcgt ccattaacga ttgccctggt cgccggagaa acctccggcg atatcctggg
    45301 ggccggttta atccgcgctc tgaaagaacg tgtgcccaac gcccgctttg ttggtgttgc
    45361 cgggccacga atgcaggctg aaggctgcga agcctggtac gaaatggaag aactggcggt
    45421 gatgggcatt gttgaagtgc tcggtcgtct gcgtcgctta ctgcatattc gtgccgatct
    45481 gacaaagcgt tttggcgaac tgaagccaga tgtttttgtt ggtattgatg cgcccgactt
    45541 caatattact cttgaaggta acctcaaaaa gcagggtatc aaaaccattc attacgtcag
    45601 tccatccgtc tgggcgtggc gacagaaacg cgttttcaaa ataggcagag ccaccgatct
    45661 ggtgctcgca tttctgcctt tcgaaaaagc gttttatgac aaatacaatg taccgtgccg
    45721 ctttatcggt cataccatgg ctgatgccat gccgttagat ccagataaaa atggtgcccg
    45781 tgatgtgctg gggatccctt acgatgccca ctgtctggca ttgttgccgg gcagccgtgg
    45841 tgcagaagtc gaaatgctta gtgccgattt cttgaaaact gcccagcttt tgcgccagac
    45901 atatccggat ctcgaaatcg tggtgccgct ggtgaatgcc aaacgccgcg agcagtttga
    45961 acgcatcaaa gctgaagtcg cgccagacct gtcggttcat ttgctggatg gaatgggccg
    46021 tgaggcgatg gtcgccagcg atgcggcact actggcgtcg gggacggcag ccctggagtg
    46081 tatgctggcg aaatgcccga tggtggtggg atatcgcatg aagcctttta ccttctggtt
    46141 ggcgaagcgg ctggtgaaaa ctgattatgt ctcgctgcca aatctgctgg cgggcagaga
    46201 gttagtcaaa gagttattgc aggaagagtg tgagccgcaa aaactggctg cggcgctgtt
    46261 accgctgttg gcgaacggga aaaccagcca cgcgatgcac gataccttcc gtgaactgca
    46321 tcagcagatc cgctgcaatg ccgatgagca ggcggcacaa gccgttctgg agttagcaca
    46381 atgatcgaat ttgtttatcc gcacacgcag ctggttgcgg gtgtggatga agtcggacgc
    46441 gggccgttag ttggcgcggt cgtcaccgct gcggtgatcc ttgacccggc gcgcccgatt
    46501 gccgggctga atgattccaa aaagctgagc gaaaaacgcc gtctggcgct ctatgaagag
    46561 atcaaagaga aagcgttgag ctggagtctg ggccgcgcgg aaccccacga aatcgacgag
    46621 ctgaacattc ttcatgcgac catgctggcg atgcagcgtg ccgtcgctgg gctgcatatt
    46681 gcgccggaat atgtgttgat tgatggtaac cgctgcccga aattaccgat gcctgcgatg
    46741 gctgtggtga aaggggatag ccgcgtacca gaaatcagtg ccgcgtctat cctggcgaaa
    46801 gtgacgcgtg acgccgaaat ggcggcgctg gatattgttt tcccgcaata tggttttgcc
    46861 caacataaag ggtatccaac cgcttttcat ctggaaaaac tggctgaata tggcgcgact
    46921 gaacatcatc gtcgcagctt tgggcctgtc aaacgcgcac tgggacttgc gtcctgattc
    46981 ttgtgtcgag attaagtaaa ccggaatctg aagatgtctg aaccacgttt cgtacacctg
    47041 cgggtgcaca gcgactactc gatgatcgat ggcttggcca aaaccgcacc gctggtaaaa
    47101 aaggcggcgg cgttgggtat gccagcactg gcgatcaccg atttcaccaa cctttgcggt
    47161 ctggtgaagt tctacggagc gggacatggc gcagggatta agcctatcgt cggggcagat
    47221 tttaatgtcc agtgcgacct gctgggtgat gagttaaccc acctgacggt actggcggcg
    47281 aacaataccg gctatcagaa tctggcgttg ctgatctcaa aagcgtatca gcgcgggtac
    47341 ggtgccgccg ggccgatcat cgatcgcgac tggcttatcg aattaaacga agggttgatc
    47401 cttctttccg gcgggcgcat gggcgacgtc ggacgcagtc ttttgcgtgg taacagcgcg
    47461 ctggtagatg agtgtgtcgc gttttatgaa gaacacttcc cggatcgcta ttttctcgag
    47521 ctgatccgca ccggcaggcc ggatgaagaa agctatctgc acgcggcggt ggaactggcg
    47581 gaagcgcgcg gtttgcccgt cgtggcgact aacgatgtgc gctttatcga cagcagcgac
    47641 tttgacgcac acgaaatccg cgtcgcgatc cacgacggct ttaccctcga cgatcctaaa
    47701 cgcccgcgta actattcgcc gcagcaatat atgcgtagcg aagaggagat gtgtgagctg
    47761 tttgccgaca tcccagaagc ccttgccaac accgttgaga tcgccaaacg ctgtaacgta
    47821 actgtgcgtc ttggtgaata cttcctgccg cagttcccga ccggggacat gagcaccgaa
    47881 gattatctgg tcaagcgtgc aaaagaaggc ctggaagagc gtctggcatt tttattccct
    47941 gacgaggaag aacgtcttaa acgccgcccg gaatatgacg aacgtctgga gactgaactt
    48001 caggttatca accagatggg cttcccgggc tacttcctca tcgttatgga atttatccag
    48061 tggtcgaaag ataacggcgt accggtaggg ccaggccgtg gctccggtgc gggttcactg
    48121 gtggcctacg cgctgaaaat caccgacctc gatccgctgg aatttgacct gctgttcgaa
    48181 cgtttcctta acccggaacg tgtctccatg cctgacttcg acgttgactt ctgtatggag
    48241 aaacgcgatc aggttatcga acacgtggcg gacatgtacg gtcgtgatgc ggtatcgcag
    48301 attatcacct tcggtacgat ggcggcgaaa gcggtgatcc gcgacgtagg ccgcgtgctg
    48361 gggcatccgt acggctttgt cgaccgtatc tcgaaactga tcccgcccga tccggggatg
    48421 acgctggcga aagcgtttga agccgagccg cagctgccgg aaatctacga agcggatgaa
    48481 gaagttaagg cgctgatcga catggcgcgc aaactggaag gggtcacccg taacgccggt
    48541 aagcacgccg gtggggtggt tatcgcgccg accaaaatta ccgattttgc gccgctttat
    48601 tgcgatgaag agggcaaaca tccggtcacc cagtttgata aaagcgacgt tgaatacgcc
    48661 ggactggtga agttcgactt ccttggtttg cgtacgctca ccatcatcaa ctgggcgctg
    48721 gagatgatca acaagcggcg ggcgaagaat ggcgagccac cgctggatat cgccgcgatc
    48781 ccgctggatg acaagaaaag cttcgacatg ctgcaacgct cggaaaccac ggcggtattc
    48841 cagcttgaat cgcgcggcat gaaggacctg atcaagcgtc tgcaacctga ctgcttcgaa
    48901 gatatgatcg cactggtggc actgttccgc cctggtccgt tgcagtcagg gatggtggat
    48961 aactttatcg accgtaaaca tggtcgtgaa gagatctcct atccggacgt gcagtggcag
    49021 catgaaagcc tgaaatcggt actggagcca acctacggca ttatcttgta tcaggaacag
    49081 gtcatgcaga ttgcccaggt gctttctggt tataccctcg gtggcgcgga tatgctgcgc
    49141 cgtgcgatgg gtaagaaaaa gccggaagag atggctaagc agcgttctgt atttgctgaa
    49201 ggtgcagaaa agaacggaat caacgccgaa ctggcgatga aaatcttcga cctggtggag
    49261 aaattcgcgg gttacggatt taacaaatcg cactctgcgg cctatgcttt ggtgtcatat
    49321 caaacgttat ggctgaaagc gcactatccg gcggagttta tggcggcggt aatgaccgcc
    49381 gatatggaca acaccgagaa ggtggtgggc ctggtggatg agtgctggcg gatggggctg
    49441 aaaatcctgc caccagatat aaactccggt ctttaccatt tccacgtcaa cgacgatggc
    49501 gaaatcgtgt atggtattgg cgccatcaaa ggggtaggtg aaggtccgat tgaggccatc
    49561 atcgaagccc gtaataaagg cggctacttc cgcgaactgt ttgatctctg cgcccgtacc
    49621 gacaccaaaa agttaaaccg gcgggtgctg gaaaaactga tcatgtccgg ggcgtttgac
    49681 cgtcttgggc cacaccgcgc ggcgctgatg aactcgctgg gtgatgcgtt aaaagcggca
    49741 gatcaacacg cgaaagcgga agctatcggt caggccgata tgttcggcgt gctggccgaa
    49801 gagccggaac aaattgaaca atcctacgcc agctgccaac cgtggccgga gcaggtggta
    49861 ttagatgggg aacgtgaaac gttaggcctg tacctgaccg gacaccctat caaccagtat
    49921 ttaaaagaga ttgagcgtta tgtcggaggc gtaaggctga aagacatgca cccgacagaa
    49981 cgtggtaaag tcatcacggc tgcggggctc gttgttgccg cgcgggttat ggtcaccaag
    50041 cgcggcaatc gtatcggtat ctgcacgctg gatgaccgtt ccgggcggct ggaagtgatg
    50101 ttgtttactg acgccctgga taaataccag caattgctgg aaaaagaccg catacttatc
    50161 gtcagcggac aggtcagctt tgatgacttc agcggtgggc ttaaaatgac cgctcgcgaa
    50221 gtgatggata ttgacgaagc ccgggaaaaa tatgctcgcg ggcttgctat ctcgctgacg
    50281 gacaggcaaa ttgatgacca gcttttaaac cgactccgtc agtctctgga accccaccgc
    50341 tctgggacaa ttccagtaca tctctactat cagagggcgg atgcacgcgc gcggttgcgt
    50401 tttggcgcga cgtggcgtgt ctctccgagc gatcgtttat taaacgatct ccgtggcctc
    50461 attggttcgg agcaggtgga actggagttt gactaataca ggaatactat gagtctgaat
    50521 ttccttgatt ttgaacagcc gattgcagag ctggaagcga aaatcgattc tctgactgcg
    50581 gttagccgtc aggatgagaa actggatatt aacatcgatg aagaagtgca tcgtctgcgt
    50641 gaaaaaagcg tagaactgac gcgtaaaatc ttcgccgatc tcggtgcatg gcagattgcg
    50701 caactggcac gccatccgca gcgtccttat accctggatt acgttcgcct ggcatttgat
    50761 gaatttgacg aactggctgg cgaccgtgcg tatgcagacg ataaagctat cgtcggtggt
    50821 atcgcccgtc tcgatggtcg tccggtgatg atcattggtc atcaaaaagg tcgtgaaacc
    50881 aaagaaaaaa ttcgccgtaa ctttggtatg ccagcgccag aaggttaccg caaagcactg
    50941 cgtctgatgc aaatggctga acgctttaag atgcctatca tcacctttat cgacaccccg
    51001 ggggcttatc ctggcgtggg cgcagaagag cgtggtcagt ctgaagccat tgcacgcaac
    51061 ctgcgtgaaa tgtctcgcct tggcgtaccg gtagtttgta cggttatcgg tgaaggtggt
    51121 tctggcggtg cgctggcgat tggcgtgggc gataaagtga atatgctgca atacagcacc
    51181 tattccgtta tctcgccgga aggttgtgcg tccattctgt ggaagagcgc cgacaaagcg
    51241 ccgctggcgg ctgaagcgat gggtatcatc gctccgcgtc tgaaagagct gaaactgatc
    51301 gactccatca tcccggaacc gctgggcggc gctcaccgta acccggaagc gatggcggca
    51361 tcgttgaaag cgcaactgct ggcggatctg gccgatctcg atgtgttaag cactgaagat
    51421 ttaaaaaatc gtcgttatca gcgcctgatg agctacggtt acgcgtaatt cgcaaaagtt
    51481 ctgaaaaagg gtcacttcgg tggccctttt ttatcgccac ggtttgagca ggctatgatt
    51541 aaggaaggat tttccaggag gaacacatga acatcattgc cattatggga ccgcatggcg
    51601 tcttttataa agatgagccc atcaaagaac tggagtcggc gctggtggcg caaggctttc
    51661 agattatctg gccacaaaac agcgttgatt tgctgaaatt tatcgaacat aaccctcgaa
    51721 tttgcggcgt gatttttgac tgggatgagt acagtctcga tttatgcagc gatatcaatc
    51781 aacttaatga atatctcccg ctttatgcct tcatcaacac ccactcgacg atggatgtca
    51841 gcgtgcagga tatgcggatg gcgctctggt tttttgaata tgcgctgggg caggcggaag
    51901 atatcgccat tcgtatgcgt cagtacacca acgaatatct tgataacatt acgccgccgt
    51961 tcacgaaagc cttgtttacc tacgtcaaag agcggaagta caccttttgt acgccggggc
    52021 atatgggcgg caccgcatat caaaaaagcc cggttggctg tctgttttat gattttttcg
    52081 gcgggaatac ccttaaggct gatgtctcta tttcggtcac cgagcttggt tcgttgcttg
    52141 accacaccgg gccacacctg gaagcggaag agtacatcgc gcggactttt ggcgcggaac
    52201 agagttatat cgttaccaac ggaatatcga cgtcgaacaa aattgtgggt atgtacgccg
    52261 cgccatccgg cagtacgctg ttgatcgacc gcaactgcca taaatcactg gcgcatctgt
    52321 tgatgatgaa cgatgtagtg ccagtctggc tgaaaccgac gcgtaatgcg ttggggattc
    52381 tgggggggat cccgcgccgt gaatttactc gcgacagcat cgaagagaaa gtcgctgcca
    52441 ctacgcaagc acaatggccg gttcatgcgg tgatcaccaa ctccacctat gatggtttgc
    52501 tctacaacac cgactggatc aaacagacgc tggatgtccc gtcgatccac ttcgattccg
    52561 cctgggtgcc gtatactcat tttcatccaa tctaccaggg taaaagtggt atgagcggcg
    52621 agcgtgttgc gggaaaagtt ttcttcgaaa cgcaatcgac ccacaaaatg ctggcggcgt
    52681 tatcgcaggc ttcactgatc cacattaaag gcgagtatga cgaagaggcg tttaacgaag
    52741 cctttatgat gcataccacc acctcgccca gttatcccat tgttgcttcg gttaagacgg
    52801 cggcggcgat gctgcgtggt aatccgggca aacggctgat taaccgttcg gtggagcggg
    52861 cgctgcattt tcgcaaagag gtccagcggc tgcgggaaga gtctgacggt tggtttttcg
    52921 atatctggca accgccgcag gtggatgaag ccgaatgcag gcccgttgcg cctggcgaac
    52981 aatggcacgg ctttaacgat gcggatgccg atcatatgtt tctcgatccg gttaaagtca
    53041 ctattttgac accggggatg gacgaacagg gcaatatgag tgaggagggg atcccggcgg
    53101 cgctggtagc aaaattcctc gacgaacgtg ggatcgtagt agagaaaacc ggcccttata
    53161 acctgctgtt tctctttagt attggcatcg ataaaaccaa agcaatggga ttattgcgtg
    53221 ggttgacgga atttaagcgc tcttacgatc tcaacctgcg gatcaaaaat atgctgcccg
    53281 atctctatgc tgaagatccc gatttctacc gcaatatgcg tattcaggat ctggcgcaag
    53341 ggatccataa gctgattcgt aaacacgatc tttccggttt gatgttgcgg gcattcgata
    53401 ctttgccgga aatgatcatg acgccacatc aggcatggca gagacagatt aaaggtgaag
    53461 tagaaaccat tgcgctggaa caactggtcg gtagagtatc ggcaaatatg atcctgcctt
    53521 atccaccggg cgtaccgctg ttgatgccag gagaaatgct gaccaaagag agccgcacgg
    53581 tactcgattt tctactgatg ctttgttccg tagggcaaca ttaccccggt tttgaaacgg
    53641 atattcacgg cgcgaaacag gacgaagacg gcgtttaccg cgtacgagtc ctaaaaatgg
    53701 cgggataact tgccagagcg gcttccgggc gagtaacgtg ctgttaacaa ataaaggaga
    53761 cgttatgctg ggtttaaaac aggttcacca tattgcgatt attgcgacgg attatgcggt
    53821 gagcaaagct ttctactgcg atattcttgg tttcacgctg caaagcgaag tctatcgcga
    53881 agcgcgcgac tcgtggaaag gggatttggc gcttaatggg caatatgtga ttgagctttt
    53941 ctcttttccg ttcccgccgg agcgccccag ccgaccggaa gcttgcggcc tgcgtcatct
    54001 ggcttttagc gttgatgaca tcgatgcggc agtggcgcac cttgaaagcc ataacgtgaa
    54061 gtgtgaagcc atccgtgtcg atccatacac gcaaaaacgc ttcaccttct ttaacgatcc
    54121 ggacgggctg ccgttggaac tgtatgagca gtaaggcttg tcatcgccgc atttgcccgg
    54181 taacgtgccg ggcattgcta ctgtaaaatc gcaccatcat gacactcacg ctcaatagac
    54241 aacttctcac ctcacgccag attctggtgg cctttagcgg cgggcttgac tccaccgttc
    54301 tgctgcatca gttggtgcag tggcggacgg aaaatccggg cgtcactctg cgcgctattc
    54361 atgtgcatca cggtttaagt gccaatgccg atgcctgggt tacgcattgc gaaaacgtct
    54421 gccaacagtg gcaggtgccg ctggtggtcg aacgcgtaca acttgcgcaa gaggggctgg
    54481 ggattgaggc ccaggcgcgg caggcacgtt atcaggcatt tgcccgtacc ctattgcccg
    54541 gcgaagtgct ggtcaccgcg caacatctcg acgatcaatg tgaaaccttt ctgctggcgc
    54601 taaaacgcgg tagcggccct gccgggcttt cggctatggc ggaagtttcg gagtttgctg
    54661 gaacacggct tatccgcccg ttgcttgccc gcacgcgggg ggaactggag cagtgggcat
    54721 tggcgcatgg tttacgctgg attgaagacg aaagtaatca ggacgactca tacgatcgta
    54781 actttctgcg cctgcgcgta gtgccgttat tgcagcagcg ttggccgcat tttgcggaag
    54841 caacggcccg cagcgccaca ctttgtgctg aacaagagag tctgttggat gaactgctgg
    54901 cggatgattt agcacactgc caaacgtcgc aggggacgct gcagattgcg ccaatgctgg
    54961 cgatgagtga tgcccgtcgc gcggcgatta ttcgccgctg gctggcaggg cagaatgcac
    55021 cgatgccttc ccgcgacgcg ctggtgcgca tctggcagga agtggcgctg gcgcgggaag
    55081 atgcttcccc ctgtttacgt ttgggcgcct ttgaaatccg ccgctatcag tcgcaactgt
    55141 ggtggattaa atccgtcacc gggcaaagcg aaaccattgt gctgtggcag acgtggctac
    55201 aaccgctgga attaccggcg gggctgggaa ccgtacagct tactgcggga ggcgatattc
    55261 gccctccgcg tgcagacgaa gcggtcagcg tgcgtttcaa agcgccagga ttgctgcata
    55321 ttgtcgggcg caacggcgga cgtaagctga agaaaatctg gcaagagtta ggcgtgccgc
    55381 cgtggctacg tgacaccacg ccactgctgt tttatggaga aacgctgatt gcggcggcag
    55441 gggtatttgt gacgcaagaa ggtgtggctg aaggtgagaa tggcgtaagt tttgtctggc
    55501 agaaaacgct tagttaagtg aaagccggat aagacgcatc aaacgtcgca tccggcgaaa
    55561 gtcaatcagg attcgcttac caccaccgta ccgatttcag ggtggctaaa gctggtaatt
    55621 ttatccagac gcagctcgcg ggtttcgcca gcggcctcga cgaccagata ctccacattt
    55681 ttgcgggaga ctaaatcact ggcttttgcc tgcaattttt cgccatcttt cagctcaagt
    55741 gtcagcatta aatgatgctg gcaggcgagc tcaagattat cgtaatcatc acaattaatt
    55801 ggttgatacg tatcattcat tgacataatc gctcaccagt aagtttgccg cagcgtatgc
    55861 tgctttttcc ctgacagcct cagaaagggc gtcgtcggca gccatttcat tcagcacttt
    55921 caaaacgcag cccagcgcgt ccggaacgta tcctaagtct ccgctggcga tttccgcgta
    55981 ccgcttgcgt attaactcac aatatttttc cacatgccct cctgtcagca ctctgactta
    56041 accgtggatg caagtctaag cctacgaaga taaactctgt ttcgcaaggt gactatacca
    56101 cactcatttc tgcaatatca gcgccgcaac tgcacgtatt ccgttacaat ggcctcctga
    56161 ttcgaaagga gttttcttat ggcgcttaaa gcgacaattt ataaagcgac ggttaatgtg
    56221 gccgatctcg accgcaacca gtttctcgat gcctctctga cgctggcgcg ccacccttca
    56281 gaaacccagg agcgtatgat gctgcgcttg ctggcgtggc tgaaatatgc tgatgaacgt
    56341 ctgcaattta cccgtggttt gtgtgccgat gatgagccgg aagcgtggct gcgtaacgat
    56401 cacctgggca ttgatttgtg gattgagctg gggctgccgg atgagcgacg aattaagaaa
    56461 gcctgcaccc aggccgcaga agtggcgctg tttacctata atagtcgggc ggcgcaaatc
    56521 tggtggcagc aaaatcagag caaatgtgtg cagtttgcca atctttccgt ctggtatctg
    56581 gacgatgaac aactggcgaa agtaagcgcc tttgccgatc gtaccatgac gctgcaggca
    56641 acgattcagg atggtgttat ctggttatcg gatgataaga ataatctgga agttaactta
    56701 accgcctggc aacaaccttc atgattgtga tttcccgaca tgttgctatt cccgatggtg
    56761 agcttgagat caccgccatt cgtgcgcagg gcgctggcgg gcagcatgtt aataagacct
    56821 caacggctat tcatctgcgt tttgacattc gggcgtccag cctgccagag tattacaaag
    56881 agcgtctgct cgccgccagc catcatttga tcagcagtga tggcgtgatt gtcattaagg
    56941 cacaggaata ccgcagtcag gaactgaacc gcgaagccgc tctggcccgg ctggtggcag
    57001 tgattaaaga tttaacaaaa gaacaaaaag cccgacgacc cacgcggccc acccgtgcat
    57061 cgaaagagcg caggctggca tcgaaagcac aaaaatcaag cgtgaaggcg atgcgcggca
    57121 aagtacgcag cggtcgggaa taaaaagaag gaatggatgg tgaaaaaagc gatagtgaca
    57181 gcgatggctg taatcagcct ctttactctg atgggatgta ataatcgggc cgaagtcgat
    57241 acgctttctc cggcgcaggc tgccgaactg aaaccgatgc cgcaaagttg gcgcggcgtg
    57301 ctaccgtgtg ccgattgcga aggaatcgaa atctctctgt tcctcgaaaa agacggaaca
    57361 tgggtgatga atgagcgtta tctcggtgct cgtgaagaac cttcctcctt cgcttcctac
    57421 ggtacatggg cgcgaaccgc tgacaagctg gtattaaccg acagcaaagg tgaaaagtca
    57481 tattatcggg cgaaaggcga tgcgctggag atgctcgatc gtgaaggcaa tccaattgaa
    57541 tcgcagttca actatacgct ggaaccggca caatccagtt tacctatgac gccgatgacc
    57601 ctgcgaggca tgtattttta tatggctgat gcggcgacct tcactgattg cgcgaccgga
    57661 aaacgtttca tggtagcgaa taacgcagag ctggagcgta gctacctggc tgcgcgcggt
    57721 cacagtgaaa aaccggtgtt actgtcagta gaaggtcact ttacgcttga ggctaatccg
    57781 gatactggtg cgccaaccaa agtattagcg cccgatacgg cgggtaaatt ttacccgaat
    57841 aaggattgca gtagtttggg gcagtaaacc tgcttaaggc agaaacaaac gcaaaattgc
    57901 ctgatgcgct gcgcttatca ggcctacagc atataatgca acattttgaa ttagcacgat
    57961 tttgtaggcc ggataaggcg tttacgccgc atccggcatt ttgtgcacga ggctggcttt
    58021 atcactgagt cagcccaaca aagcgacccg ccttgatata aatcccaggc ttcaggtgcc
    58081 caacatactg taactgcgtt tctggcttaa acgcagagac atcaccaata cgcatatgca
    58141 tcagatcggc agggctaatc cagcccgatt gcgccagtgt aagtggatgc ccggatttgg
    58201 caaatgcatc agtgacaaat tccgaacaaa accacgactt tttgtctcct tcgcacacac
    58261 tgcttaactg cgctttcgcc aggtcgctga cgcactgttg gcgaaaatcc tcggagaacg
    58321 gattcagtga gcacatctgg cgagtcacca taaagggaat aaattcgaca atgccgcgat
    58381 agttataacc gctatctttg attttattgg caaacgcggt gatatctgtg gcttgttgcg
    58441 gggtaagatc cgggactcgt aagacgaaaa gcttatcact atgcttcatc gcttttttaa
    58501 gggaaacaat ctggacgcca gcgcctgtcg cttctgcaac gttattatca cccagaaaga
    58561 ttgcaacgtg actcacagag gaagtgctga agacgcggat tccaaatgag gttaccccaa
    58621 ggcttgagga gaacagcaaa tcgccgggtt tgagatcagg tgccgttatt tcttttattg
    58681 attgttcggt gaaagagctt tgatgctgga atttgacagc ccatgttttt gcctcggcat
    58741 ctactgctgt ggctgatggg tctggctggc tgatatcaac ggtacaagcc gaaagtaaaa
    58801 gaaagctggg gagaagcagg cggcagtacg cctttggttt atccatttta tacaatccat
    58861 gtaaaaaagg gccctgaaat tcaggaccct ttctggcatc agcctttaat ctgtttcacc
    58921 agatattcga cgatgtcacc agtcttaatt aactgtttct cgccgttgcg acgatattta
    58981 tattcgatat cgtcgttgtc gaggttacgg tcgcccagca caatagtgtg cggaataccg
    59041 atcagttcca tatcagcaaa catcacgccc ggacgctctt tgcggtcatc cagcagcact
    59101 tcgatacctt gtgcacgcag ttcgctgtac agtttctcag caagctcttg tacgcggaag
    59161 gatttgtgca tgttcatcgg cagaatcgcc acctggaacg gcgcgatagc gtcaggccat
    59221 acgatgccgc gttcgtcgta gttctgctca atcgccgcag ctaccacacg cgttaccccg
    59281 ataccgtagc aacccatcgt caggatttgg ttacggccat cttcaccctg tacggaggct
    59341 ttcagtgctt cggagtactt ggtacccagc tggaagatgt gaccaacttc gataccacgt
    59401 ttgatcagca gcgtaccctg gccatccggg cttggatcgc cagccaccac gttacggata
    59461 tctgcaactt ccggggtagc gacatcgcga tcccagttga taccgaagta gtgtttacca
    59521 tcgatgttag caccagcagc gaaatcactc atcgccgcaa cggtacggtc aatcaccacc
    59581 ggaatcggca tgtttaccgg acccagtgaa cccggaccgg ctttaaccac agcacgaatt
    59641 tcttcttcgg tcgcgaaagt cagcgggctt gcaacctgcg gcagtttttc tgctttaact
    59701 tcgttcagct cgtgatcacc gcgcaccagc agcgcaacca gcgggaagct actgccttca
    59761 accgctttaa ccagcagagt cttaaccgtt ttctcaatcg gcagattgaa ctgttcaacc
    59821 agttccgcga tggttttcgc gttcggcgta tcaaccagcg tcatttcctg ggtagcagca
    59881 gcgcgcggtt ctttcggcgc gatagcttct gccagttcaa tgttcgctgc atagtcagag
    59941 gtgtcggaga agaccacatc gtcttcaccg ctctgcgcca gcacctggaa ttcgtgagag
    60001 gcgctgccgc cgatagaacc ggtgtcggcc tgtacggcgc ggaaatccag ccccatgcgg
    60061 ctgaagattt tgctgtaggc cgcatacatt gcatcgtagg tttcctgcag ggattcctga
    60121 gaagtatgga aagagtaagc atctttcatc aggaattcgc gggaacgcat gacgccgaaa
    60181 cgcggacgca cttcgtcgcg gaacttggtc tggatctgat agaagttcag cggcagctgt
    60241 ttgtaagagc taagctcgtt acgaatcagg tcagtgataa cttcttcatg agttgggccg
    60301 agtacgaacg gacgctcgcc acggtcaaca aaacgcagca gttccggacc gtactgttcc
    60361 caacgaccac tctcttgcca caaatcggct ggctgaacca ccggcatcga cacctcgatc
    60421 gcaccggcgt tgttcatctc ttcacgcacg atgttttcga cttttttcag aacgcgcacg
    60481 ccggtcggca gccaggtata taacccggag gccagcttgc ggatcatccc ggcgcgcagc
    60541 atcagctgat ggctgatcac ctcggcgtcg gcaggtgtct ccttgagagt ggagagcagg
    60601 tattggctag tacgcatgtt gttacggttc cagttggaag gtagaacagg ctcaaggcga
    60661 gcctgggaca aaaaaagtga tttagtttac cagtgcaaaa gaaatgtcaa aagagaaggg
    60721 cgtgaattta acgcggttcc agcgcaaaga cttcaaaacc tgcgtcggtg acgcgccagc
    60781 gaacgttaaa atcatgtagc cagacggcat aggttttgcc cgtttcctca cctttacgat
    60841 aggccgggcg cgggtcctgc gccagtactt cgcggataaa cagcgttaac tgcggataac
    60901 gcttctccag cgtcaaaagc tgcttttcga cctctgcggt aaaactcacc gccatctctg
    60961 cagctggcgc actttgcgca tagctggcac tggcatcggg aagcgattcg gcaaagggga
    61021 gatacggttt gatatccact accggcgtac catcgaccag atccagactg ccgagcttca
    61081 gaatcacgct gcctttatgg caaacaacct ctttcagctc taccagcgac atgccaattg
    61141 ggttagggcg gaaagtagag cgtgttgcga aaacccccat tctggcgtta ccgccgaggc
    61201 gcggtggacg cacagtcgga cgccagccgc cttccatcgt ttgatgaaag acgaaaagga
    61261 tccataaatg gctgaatgct tccaggccgc gaacggcgtc ggcctggttg taggaagcta
    61321 tgagatgcag ttcgccgttg gcgcttttta ccagacctgg ctggcgcgga acggcgaatt
    61381 tttctttata gggcgagcga ataacgccta tttgctcgaa ctggaaactg ctcatttcgc
    61441 cgtaatgtta agcgcagagc cgatacatac agcctgacga tagcagcccg gcgtaccgct
    61501 ggtgacttcg cagctatgca gtaatacagc attggctttc attttagagg cattgatttg
    61561 catctgctta cgtgcggttg gaatgctcgg cggagagtcc tgattagagg cctggcaaga
    61621 gtcgccactg acttcaccga gatcgcggaa cggtttgccg actaattctt ctgcattggt
    61681 ataaattcgg accggcgtgg cgcgcggcgc tttcggtttt gcaggctccg ctttcggctg
    61741 gggtgcagtg ctttgaacgg gttcgacagg ggatctgctt aacatggaac agccgcttag
    61801 catgagtgct actaaacaga tcggtaaagc acgcatagta tttcctcaat gtatgatcaa
    61861 aacgtcaata ttgaatcagg agcttgtaaa aatgacaaga cgggcaagcg cccgtcctga
    61921 atgatattac aaattgtgga aacagcctaa aaattaccag cctttaacag cgccgccgtt
    61981 aaacactttg tttgctgctt cgtaaacttc atcagactga taagcctgga cgaatttctt
    62041 cacgttctcg gcgtctttgt tatcttcacg cgttacgatc aggtttacgt atggggagtc
    62101 tttatcttcg acaaagatac cgtctttcgc cggagtcagg ccaatctggc tggcataggt
    62161 ggtattgata actgccagag cgatttgcgc gtcgtccaga gaacgcggca gttgcggtgc
    62221 ttccagttca acaattttca gattttttgg gttctcaaca acatcaagaa cggtcggcag
    62281 caggccaacg ccatctttca gtttgatcaa gcccactttt tgcagcagca gcagtgaacg
    62341 accaagatta gttggatcgt ttggcacggc aacctgcgaa ccatcctgca gttcatccag
    62401 tgatttgatt ttcttggagt aaccagcaat cggataaaca aaagtgttgc ctactgcgac
    62461 cagtttgtag ccacgatctt tcagttgctg atcaaggtac ggtttatgct ggaaggcgtt
    62521 ggcgtcgata tcgcctttgc tcaatgcttc gtttggcaga acatagtcgt tgaaggttac
    62581 cagctcaacg tccaggccat atttgtcttt cgcaactttc tgcgcgactt ctgctacctg
    62641 ctgttcggca ccaacaatca cgccgacttt aatgtggttt ggatcttttt catcctgacc
    62701 gcagcctacc agtgccagtg atccaatcag ggctcccact gccgcaaagg ttttgaattt
    62761 gaacgccata ccttattcct tcttcaatta tttatgttgt gttgaacgtt acttgcgagt
    62821 gacagcccgg acgatgcggt cgcctgcaaa ctgaattaaa taaaccagaa tgaccagcaa
    62881 taccagtacc gtattcatca ccgtcgcgtt gtagccgatg tagccatact gatagccaat
    62941 ctgacctaaa ccaccggcac cgactgcacc acccatcgcg gaataaccga ccagggtaat
    63001 cagggtgata gttgccgcat tcaccagacc cggcaacgct tccggtaaca gcactttaca
    63061 gacgatctgc attggcgtgg cacccattgc gcgggaagct tcaattaacc cggttgggat
    63121 ctccagcaga gcgttctcga ccatacgggc aataaacggt gctgcaccaa cggttaacgg
    63181 aacaattgct gcctgcaaac caatcgatgt accgacaata acgcgggtaa acggaatcat
    63241 ccataccagc aagataatga acgggatgga acggaaaatg ttcacaatcg cagaaacggt
    63301 acgatacagc ttcgcgttag caataatttg ccccggacgc gtgacataaa gcagaacgcc
    63361 aaccggcaga ccaatcacaa acccaaaaaa tccggatacg aaggtcattg ccagcgtttc
    63421 ccatacgcca cgaaccagca gccacatcat cggctcagac ataacccagt acctctactt
    63481 ttacatggtg ttcctgcagc caggcaatgg cggcttgcgt atcttgttgt gtgccgtgca
    63541 tttcagtcag catgatgccg aatttaacgc caccggcgta gtccatctgc gcgctaataa
    63601 tgttgttgtt gacgttgaaa cgacgcgcgg tttcagaaag cagtggggca tcgaccgatt
    63661 gaccggtaaa ctccagacgc agcatcggca cgcagtcagt aaatggctcc gtttgcagac
    63721 gttcctggta atcttccggg atatccagat gcagggtcga ctgaataaac ttctgcgcca
    63781 gcggcgtttt cggatgcgag aacacttcac ttaccgtgtc ctgctcgatc agttcgccat
    63841 tgctgatgac cgccacgcaa tcacaaatac gcttcacaac gtccatttcg tgggtgatca
    63901 acagaatcgt caaacccaga cggcggttaa tgtctttcag cagttcgaga atagaacgtg
    63961 tcgttgccgg gtccagcgcg ctggtggctt catcacacag caatactttg ggattgctgg
    64021 ctaatgcacg ggcaatcgcc acacgttgtt tctgcccacc ggaaagattc gacgggtagc
    64081 tatcatgctt atcgccaaga ccaaccaatg acagcaattc cgtcacgcga cgtttgatct
    64141 cgtctttcgg tgtgttgtcc agctccagcg gcagagccac gttgccaaaa acagtacgcg
    64201 aagagagcag gttaaaatgc tggaaaatca taccaatctg gcggcgagct ttggtcaact
    64261 cggattctga cagcgtggtc aattcctggc catcgaccag cacgctaccc tcggttgggc
    64321 gctccagcaa gtttacacaa cgtataagcg tactcttacc cgcgcctgag gcaccgataa
    64381 cgccataaat ttgtccagct ggcacatgca ggctgacgtt gttcaacgcc tggatggtgc
    64441 gggtgccctg gtggaacact ttggtgatat tcgaaagttt tatcattgat tatttattat
    64501 cgtcattaag ttagtcgtag catctcgaat gcctgaaacg ggcaacgccg tcaatgaaat
    64561 ggatgttaag gcatccagac gtctaaatca atcaggttta tgcgaagagc actttcttgc
    64621 aggtcgaaac atgcgatact agcgtcccat gccttattaa ggagctataa aaggtggcga
    64681 agagcgtacc cgcaattttt cttgaccgtg atggcaccat taatgtcgat cacggctatg
    64741 tccatgagat cgacaacttt gaatttatcg acggtgttat tgacgccatg cgcgagctaa
    64801 aaaaaatggg ctttgcgctg gtggtagtaa ccaaccagtc tggcattgct cgcggtaaat
    64861 ttaccgaagc acagtttgaa acgctgaccg agtggatgga ctggtcgctg gcggaccgag
    64921 atgtcgatct ggatggtatc tattattgcc cgcatcatcc gcaggggagt gttgaagagt
    64981 ttcgccaggt ctgcgattgc cgcaaaccac atccggggat gcttttgtca gcacgcgatt
    65041 atttgcatat tgatatggcc gcttcttata tggtgggcga taaattagaa gatatgcagg
    65101 cagcggttgc ggcgaacgtg ggaacaaaag tgctggtgcg tacgggtaaa cctattacac
    65161 ctgaagcaga aaacgcggca gattgggtgt taaatagcct ggcagacctg ccgcaagcga
    65221 taaaaaagca gcaaaaaccg gcgcaatgat taaaagatga acggttgaaa taaaaatgca
    65281 tttttccgct tgtcttcctg agccgactcc ctataatgcg cctccatcga cacggcggat
    65341 gtgaatcact tcacacaaac agccggttcg gttgaagaga aaaaatcctg aaattcaggg
    65401 ttgactctga aataggaaag tgtgatatac gccacctcgc gacagtgagc tgaaagctgc
    65461 gtcgcaactg ctctttaaca atttatcaga caatctgtgt gggcactcga agatacggat
    65521 tcttaacgtc gcaagacgat aaatgaatac caagtctcaa gagtgaacac gtaattcatt
    65581 acgaagttta attctttgag catcaaactt ttaaattgaa gagtttgatc atggctcaga
    65641 ttgaacgctg gcggcaggcc taacacatgc aagtcgaacg gtaacaggaa gcgcttgctg
    65701 cttcgctgac gagtggcgga cgggtgagta atgtctggga aactgcctga tggaggggga
    65761 taactactgg aaacggtagc taataccgca taacgtcgca agaccaaaga gggggacctt
    65821 cgggcctctt gccatcggat gtgcccagat gggattagct tgttggtggg gtaacggctc
    65881 acc
//
LOCUS       DAZSIO010000003        55497 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZSIO010000003 DAZSIO010000000
VERSION     DAZSIO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.003"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            99..1585
                     /locus_tag="ACHX5T_000135"
                     /pseudo
     CDS             99..1585
                     /locus_tag="ACHX5T_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
     gene            1600..2334
                     /gene="ypdB"
                     /locus_tag="ACHX5T_000136"
     CDS             1600..2334
                     /gene="ypdB"
                     /locus_tag="ACHX5T_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR8465513.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2347..3204
                     /locus_tag="ACHX5T_000137"
                     /pseudo
     CDS             2347..3204
                     /locus_tag="ACHX5T_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(3207..5709)
                     /gene="ptsP"
                     /locus_tag="ACHX5T_000138"
                     /pseudo
     CDS             complement(3207..5709)
                     /gene="ptsP"
                     /locus_tag="ACHX5T_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5734..6778)
                     /gene="ypdE"
                     /locus_tag="ACHX5T_000139"
                     /pseudo
     CDS             complement(5734..6778)
                     /gene="ypdE"
                     /locus_tag="ACHX5T_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(6778..7194)
                     /locus_tag="ACHX5T_000140"
     CDS             complement(6778..7194)
                     /locus_tag="ACHX5T_000140"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFR8465514.1"
                     /translation="MTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQ
                     QIDDAARRVITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIY
                     LLGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(7191..7298)
                     /locus_tag="ACHX5T_000141"
     CDS             complement(7191..7298)
                     /locus_tag="ACHX5T_000141"
                     /inference="COORDINATES: protein motif:HMM:NF019360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8465515.1"
                     /translation="MARGAAARQSQRQDCCSGRVCHSRFWCAVSGLLLI"
     gene            complement(7259..7861)
                     /gene="ypdF"
                     /locus_tag="ACHX5T_000142"
                     /pseudo
     CDS             complement(7259..7861)
                     /gene="ypdF"
                     /locus_tag="ACHX5T_000142"
                     /inference="COORDINATES: protein motif:HMM:NF007310.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7876..9123)
                     /locus_tag="ACHX5T_000143"
     CDS             complement(7876..9123)
                     /locus_tag="ACHX5T_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFR8465516.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9145..9471)
                     /locus_tag="ACHX5T_000144"
     CDS             complement(9145..9471)
                     /locus_tag="ACHX5T_000144"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR8465517.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9690..10655)
                     /gene="glk"
                     /locus_tag="ACHX5T_000145"
     CDS             complement(9690..10655)
                     /gene="glk"
                     /locus_tag="ACHX5T_000145"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR8465518.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            10859..12115
                     /locus_tag="ACHX5T_000146"
     CDS             10859..12115
                     /locus_tag="ACHX5T_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR8465519.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            12230..12556
                     /gene="ypeC"
                     /locus_tag="ACHX5T_000147"
     CDS             12230..12556
                     /gene="ypeC"
                     /locus_tag="ACHX5T_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR8465520.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12697..13935)
                     /locus_tag="ACHX5T_000148"
     CDS             complement(12697..13935)
                     /locus_tag="ACHX5T_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR8465521.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14271..15473
                     /gene="nupC"
                     /locus_tag="ACHX5T_000149"
     CDS             14271..15473
                     /gene="nupC"
                     /locus_tag="ACHX5T_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR8465522.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15523..17635)
                     /locus_tag="ACHX5T_000150"
                     /pseudo
     CDS             complement(15523..17635)
                     /locus_tag="ACHX5T_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(17843..17918)
                     /locus_tag="ACHX5T_000151"
     tRNA            complement(17843..17918)
                     /locus_tag="ACHX5T_000151"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17883..17885),aa:Ala,seq:ggc)
     gene            complement(17958..18033)
                     /locus_tag="ACHX5T_000152"
     tRNA            complement(17958..18033)
                     /locus_tag="ACHX5T_000152"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17998..18000),aa:Ala,seq:ggc)
     gene            18254..18613
                     /locus_tag="ACHX5T_000153"
     CDS             18254..18613
                     /locus_tag="ACHX5T_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR8465523.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18615..19007
                     /locus_tag="ACHX5T_000154"
     CDS             18615..19007
                     /locus_tag="ACHX5T_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR8465524.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(19059..20474)
                     /gene="gltX"
                     /locus_tag="ACHX5T_000155"
     CDS             complement(19059..20474)
                     /gene="gltX"
                     /locus_tag="ACHX5T_000155"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR8465525.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            20733..20808
                     /locus_tag="ACHX5T_000156"
     tRNA            20733..20808
                     /locus_tag="ACHX5T_000156"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20766..20768,aa:Val,seq:tac)
     gene            20853..20928
                     /locus_tag="ACHX5T_000157"
     tRNA            20853..20928
                     /locus_tag="ACHX5T_000157"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20886..20888,aa:Val,seq:tac)
     gene            20933..21008
                     /locus_tag="ACHX5T_000158"
     tRNA            20933..21008
                     /locus_tag="ACHX5T_000158"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20966..20968,aa:Lys,seq:ttt)
     gene            21039..21164
                     /locus_tag="ACHX5T_000159"
     ncRNA           21039..21164
                     /ncRNA_class="other"
                     /locus_tag="ACHX5T_000159"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            21284..21625
                     /locus_tag="ACHX5T_000160"
     CDS             21284..21625
                     /locus_tag="ACHX5T_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR8465526.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(21616..22542)
                     /locus_tag="ACHX5T_000161"
     CDS             complement(21616..22542)
                     /locus_tag="ACHX5T_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8465527.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            22632..23630
                     /locus_tag="ACHX5T_000162"
     CDS             22632..23630
                     /locus_tag="ACHX5T_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR8465528.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(23627..23845)
                     /locus_tag="ACHX5T_000163"
     CDS             complement(23627..23845)
                     /locus_tag="ACHX5T_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR8465529.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(23847..25862)
                     /gene="ligA"
                     /locus_tag="ACHX5T_000164"
     CDS             complement(23847..25862)
                     /gene="ligA"
                     /locus_tag="ACHX5T_000164"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR8465530.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(25933..26919)
                     /gene="zipA"
                     /locus_tag="ACHX5T_000165"
     CDS             complement(25933..26919)
                     /gene="zipA"
                     /locus_tag="ACHX5T_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR8465531.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            27149..27910
                     /gene="cysZ"
                     /locus_tag="ACHX5T_000166"
     CDS             27149..27910
                     /gene="cysZ"
                     /locus_tag="ACHX5T_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR8465532.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            28095..29066
                     /gene="cysK"
                     /locus_tag="ACHX5T_000167"
     CDS             28095..29066
                     /gene="cysK"
                     /locus_tag="ACHX5T_000167"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR8465533.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            29450..29707
                     /gene="ptsH"
                     /locus_tag="ACHX5T_000168"
     CDS             29450..29707
                     /gene="ptsH"
                     /locus_tag="ACHX5T_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR8465534.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            29752..31479
                     /gene="ptsI"
                     /locus_tag="ACHX5T_000169"
     CDS             29752..31479
                     /gene="ptsI"
                     /locus_tag="ACHX5T_000169"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR8465535.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            31520..32029
                     /gene="crr"
                     /locus_tag="ACHX5T_000170"
     CDS             31520..32029
                     /gene="crr"
                     /locus_tag="ACHX5T_000170"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR8465536.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(32071..32922)
                     /gene="pdxK"
                     /locus_tag="ACHX5T_000171"
                     /gene_synonym="thiJ"
     CDS             complement(32071..32922)
                     /gene="pdxK"
                     /locus_tag="ACHX5T_000171"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR8465537.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            33027..33401
                     /locus_tag="ACHX5T_000172"
     CDS             33027..33401
                     /locus_tag="ACHX5T_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR8465538.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            33434..34157
                     /locus_tag="ACHX5T_000173"
                     /pseudo
     CDS             33434..34157
                     /locus_tag="ACHX5T_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(34361..35272)
                     /gene="cysM"
                     /locus_tag="ACHX5T_000174"
     CDS             complement(34361..35272)
                     /gene="cysM"
                     /locus_tag="ACHX5T_000174"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR8465539.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(35406..36503)
                     /gene="cysA"
                     /locus_tag="ACHX5T_000175"
     CDS             complement(35406..36503)
                     /gene="cysA"
                     /locus_tag="ACHX5T_000175"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR8465540.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(36493..37368)
                     /gene="cysW"
                     /locus_tag="ACHX5T_000176"
     CDS             complement(36493..37368)
                     /gene="cysW"
                     /locus_tag="ACHX5T_000176"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR8465541.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(37368..38201)
                     /gene="cysT"
                     /locus_tag="ACHX5T_000177"
     CDS             complement(37368..38201)
                     /gene="cysT"
                     /locus_tag="ACHX5T_000177"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR8465542.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(38201..39217)
                     /gene="cysP"
                     /locus_tag="ACHX5T_000178"
     CDS             complement(38201..39217)
                     /gene="cysP"
                     /locus_tag="ACHX5T_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR8465543.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(39521..40312)
                     /gene="ucpA"
                     /locus_tag="ACHX5T_000179"
     CDS             complement(39521..40312)
                     /gene="ucpA"
                     /locus_tag="ACHX5T_000179"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR8465544.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(40441..41299)
                     /gene="murR"
                     /locus_tag="ACHX5T_000180"
                     /pseudo
     CDS             complement(40441..41299)
                     /gene="murR"
                     /locus_tag="ACHX5T_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
     gene            41463..42359
                     /gene="murQ"
                     /locus_tag="ACHX5T_000181"
     CDS             41463..42359
                     /gene="murQ"
                     /locus_tag="ACHX5T_000181"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR8465545.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            42363..43787
                     /gene="murP"
                     /locus_tag="ACHX5T_000182"
     CDS             42363..43787
                     /gene="murP"
                     /locus_tag="ACHX5T_000182"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFR8465546.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            43792..43878
                     /locus_tag="ACHX5T_000183"
                     /pseudo
     CDS             43792..43878
                     /locus_tag="ACHX5T_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(43967..44866)
                     /gene="yfeX"
                     /locus_tag="ACHX5T_000184"
     CDS             complement(43967..44866)
                     /gene="yfeX"
                     /locus_tag="ACHX5T_000184"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR8465547.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(44962..45537)
                     /locus_tag="ACHX5T_000185"
     CDS             complement(44962..45537)
                     /locus_tag="ACHX5T_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR8465548.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(45598..46047)
                     /locus_tag="ACHX5T_000186"
     CDS             complement(45598..46047)
                     /locus_tag="ACHX5T_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR8465549.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(46034..46459)
                     /locus_tag="ACHX5T_000187"
     CDS             complement(46034..46459)
                     /locus_tag="ACHX5T_000187"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR8465550.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            46673..47542
                     /gene="amiA"
                     /locus_tag="ACHX5T_000188"
     CDS             46673..47542
                     /gene="amiA"
                     /locus_tag="ACHX5T_000188"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR8465551.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            47546..48445
                     /gene="hemF"
                     /locus_tag="ACHX5T_000189"
     CDS             47546..48445
                     /gene="hemF"
                     /locus_tag="ACHX5T_000189"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR8465552.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(48451..49503)
                     /gene="eutR"
                     /locus_tag="ACHX5T_000190"
                     /pseudo
     CDS             complement(48451..49503)
                     /gene="eutR"
                     /locus_tag="ACHX5T_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(49549..50049)
                     /gene="eutK"
                     /locus_tag="ACHX5T_000191"
     CDS             complement(49549..50049)
                     /gene="eutK"
                     /locus_tag="ACHX5T_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR8465553.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(50062..50721)
                     /gene="eutL"
                     /locus_tag="ACHX5T_000192"
     CDS             complement(50062..50721)
                     /gene="eutL"
                     /locus_tag="ACHX5T_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR8465554.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(50731..50799)
                     /locus_tag="ACHX5T_000193"
                     /pseudo
     CDS             complement(50731..50799)
                     /locus_tag="ACHX5T_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            51903..52694
                     /locus_tag="ACHX5T_000194"
     CDS             51903..52694
                     /locus_tag="ACHX5T_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8465555.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            53414..53809
                     /locus_tag="ACHX5T_000195"
     CDS             53414..53809
                     /locus_tag="ACHX5T_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR8465556.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            53827..54090
                     /locus_tag="ACHX5T_000196"
     CDS             53827..54090
                     /locus_tag="ACHX5T_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8465557.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            54113..54655
                     /locus_tag="ACHX5T_000197"
     CDS             54113..54655
                     /locus_tag="ACHX5T_000197"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8465558.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            54794..55081
                     /locus_tag="ACHX5T_000198"
     CDS             54794..55081
                     /locus_tag="ACHX5T_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8465559.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            55120..55326
                     /locus_tag="ACHX5T_000199"
     CDS             55120..55326
                     /locus_tag="ACHX5T_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8465560.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
BASE COUNT        13842 a        14729 c        13726 g        13200 t
ORIGIN      
        1 ccccttttaa atttatgttt tttcctcaaa aacggcctgc tggtcatcag ccttcaggtt
       61 acctatcata gaggtttaat ccttattcag agtcacccgt gcacgaaata ttcaacatgc
      121 tgctggcggt cttcgatcgg gcagcgttaa tgcttatctg cctgttcttt ctcatccgta
      181 tccgcctgtt tcgcgaactg ttgcacaagt cggcgcactc cccaaaagaa ttgctcgccg
      241 ttaccgccat tttctcgctg ttcgccctgt tcagcacctg gtccggcgtt cccgtagaag
      301 gctcgctggt gaacgtacgt attatcgcgg tgatgtccgg cgggattctg tttggcccgt
      361 gggtaggcat cattaccggc gtgattgcgg gtattcaccg gtatttaatt gatatcggcg
      421 gcgtgacggc gatcccctgc tttatcacca gcattctggc gggttgtata tcaggctgga
      481 taaacctgaa aatccccaaa gcacagcgct ggcgctggat atcgccaaca aaacgctacc
      541 gctgtttcgc catgtcaata gcgagtcatt acgcaaggtc tgcgaaatta tccgcgatga
      601 cattcacgcc gatgcggtgg cgattactaa taccgatcat gtgctggcct atgttggcgt
      661 gggtgaacat aactatcaga atggcgatga cttcattagc ccgactaccc gtcaggcgat
      721 gaattacgga aaaatcatca ttaaaaacaa tgatgaagcc caccgcacac cagagattca
      781 ttccatgctg gtgatcccat tgtgggagaa aggggtcgtg accggaacgc tgaaaattta
      841 ctactgccac gcgcatcaga tcacctcgtc attacaggaa atggcggtcg gtctgtcgca
      901 aatcatctcc acgcaactgg aggtttcacg cgccgagcag ctacgtgaaa tggcaaataa
      961 ggcagagatt cgcgccctgc aaagcaaaat taatccccat tttctgttta acgctctgaa
     1021 cgctatttca tcgtcaatcc gtctgaatcc ggataccgct cgccagttga tctttaatct
     1081 gtcgcgttat ctgcgctata acattgaatt aaaagacgat gagcaaatcg atatcaaaaa
     1141 agagctgtat caaattaaag actatattgc cattgagcag gcccgctttg gcgacaagct
     1201 gacggttatc tatgatattg atgaagaggt gaattgctgc attcccagcc tgctgatcca
     1261 gccgttggtg gagaacgcca ttgtccacgg tattcagcct tgcaaaggta aaggcgttgt
     1321 caccatcagc gttgcagagt gcggaaatcg ggtacgcatt gcggtgcgag ataccgggca
     1381 cggcatcgtt ccaaaggtga ttgagcgggt cgaagcgaat gaaatgccgg gcaataaaat
     1441 tggcctgctg aatgtccatc atcgcgtgaa gttattgtat ggcgaggggc tgcatatccg
     1501 ccgcctggag ccggggacgg aaattgcgtt ttacattcct aaccaacgca ccccagtcgc
     1561 ctcacaggct acgttattgc tttgagccgg agtgatattg tgaaagtcat cattgttgaa
     1621 gacgaattcc tggcacaaca ggaactgagc tggctaatta aagagcacag ccagatggag
     1681 attgtcggca cctttgacga cggtctggac gtgttgaagt ttttgcagca taaccgcgtc
     1741 gacgccattt ttctggatat caatattccg tcgctggatg gcgtgttgct ggcgcaaaac
     1801 atcagccagt tcgcccataa accgtttatt gtgttcatca ccgcgtggaa agaacatgcg
     1861 gtagaagcgt ttgaactgga ggcgtttgac tacattctca aaccgtatca ggagtcacgt
     1921 attaccggga tgctgcaaaa actggaagcg gcctggcaac aacagcagac cagcagtacg
     1981 cctgccgcga cggtaacgcg tgagaatgac accattaatc tggtgaaaga tgagcgaata
     2041 atcgtcacgc caattaacga tatctattac gccgaagcgc acgagaaaat gacctttgtc
     2101 tatacgcggc gtgaatccta cgtaatgccg atgaacatta ccgaattttg cagcaaactg
     2161 ccgccgtcgc attttttccg ctgccatcgc tcattttgtg tcaatctgaa caaaatacgc
     2221 gaaatcgaac cgtggtttaa taacacctac attctgcgac tgaaagatct ggattttgaa
     2281 gtgccggtca gccgcagcaa agtgaaagaa tttcgccagt taatgcatct ttaatcggga
     2341 actttcatga aagcaccagg cttgcccgcc gatcaacaat ttttcgccga tctgttcagc
     2401 ggcctagtgc ttaacccgca actactgggg cgcgtctggt ttgccagcca gcctgcctcg
     2461 ttaccggtgg gcagtttatg tattgatttt ccccgtctgg atatcgtgct gcgcggcgaa
     2521 tacggcaacc tgctggaagc aaagcagcag cgaatggtgg aaggagaaat gctgtttatt
     2581 ccggcacgcg cggctaattt accgatcaac aacaaaccgg tgatgctgtt aagcctggtg
     2641 ttcgcaccga cctggcttgg gttatcgttt tacgatagcc gcaccacgtc gttgttgcat
     2701 cctgctcgcc agacccagct tcccagcctg caacgcggtg aaggtgaagc gatgcttacc
     2761 gccctcaccc atcttagccg ttcgccgctg gagcagaata tcattcagcc actggtgtta
     2821 agtttgctgc atctttgccg caacgtggtg aatatgccgc cgggtaattc acaaccgcgc
     2881 ggcgattttc tctatcacag catttgtaac tgggtttagg ataattatgc ccagccgctc
     2941 acccgcgaga gcgtggcgca gttttttaat atcacgccca atcatctgtc aaaactgttt
     3001 gctcagcatg gaacgatgcg ttttatcgag tatgtgcgtt gggtgcgaat ggcgaaggcg
     3061 aggatgattt tgcagaaata tcatctgtca attcatgaag tggcacagcg ttgcggtttt
     3121 ccggatagcg actatttttg tcgcgttttc cggcgtcagt ttagtctgac gccgggagag
     3181 tacagcgccc gttttcaggg ctaacgtcag aaggttaatt ctgtttccag cagcgtcagg
     3241 atactttgtg catcttgcgc ggcaaacaac gactggcgga agtttttatt caccagttta
     3301 cgcgccagct gcgagaagac tttcacatga ttcatccctt cgttagcacc cagcgtcagc
     3361 atgatcacca gttcgacttc gcccatttct gactgccagt caaccggttt cgccagccgg
     3421 gcaatgctga tactggaatg acggatccac tgagatttgg tgtgcgggat cgctacgcca
     3481 aaaccaacgc cggtggtaac aatctcttcc cgctgccaga catcttcttc cagctcaaac
     3541 ggatgttcag tgcgcccgtt aacgccgagg ttgccgcaca ggaactggat cgcctgctct
     3601 ttattgctaa aatcctgatc aacaaagata ttctccagcg ccagcagtgg gcgaacgtct
     3661 tcttccggcg taaaggcggt gagtaacgct tcaatttcct gcgcactgcg gcattcacat
     3721 gcctgacgcg ccagttcccg acacgcctcg ctatccagtt gacgaagctg gcttttcacc
     3781 gccggaatac gcgggctact catactcagc tcatccaggc ccagcccaag cagtagcggc
     3841 agataacggc tttcaccgcc cagttcaccg caaatgccta cccatttgcc ccgctgatgc
     3901 gcagtggtaa ctatttgctg caacatgcgc aggaacgatg gcgtaatcgg gttatatagc
     3961 ggcgatacgc gcgggttatt acgatcgacc gcatacagat actgggtcat atcgttggag
     4021 ccgatactga agaaatcgac ctcatcgcag aagtggtcga tgatgtagca caccgacggc
     4081 acttccacca tgatcccaag cgtaatcgtc tctgcatgac gcaggccatc gcgcttaagc
     4141 tcaacgatcg ctttttggat ctcgcctttc acccataaga tctgatcgag gctgtgaacc
     4201 atcgggatca tcaactgggc gttgccgaaa ctggcggcgc gcaaaatggc ccgcagttga
     4261 gtgcggaaca ggccagcaaa ttccgggtaa atacgtaccg cgcgatagcc gaggaacggg
     4321 ttctcttcct ggggaatatt cagataagga atgcttttat cgccgccgat gtccatcgtg
     4381 cggaagataa tcggcttgtc gcccgccgcc cgccgccagt agcacctgct ggtaggcttc
     4441 aaattgctcc tgctcgtccg gcgcgctgtc gcgatccata tagagcattt cggtacggaa
     4501 cagcccgaca ccttccgcgc cgttggcaaa cgcgcctggc gcttccagag cggtgccgat
     4561 attcgccgca atatcaatac gcttgttatc acgggaatag gccagctgcg cggcagcctg
     4621 cgcctgttgt ttttggcgtt tatccgccag cgtctgcgcg acctgataat aaccgctcac
     4681 cgcgtcattc gggttaatcg ccagcacgcc gcactgggcg tcaagcactg caggttgccc
     4741 ggcataacgg gcaatcgcat ccagcggcaa gccactcagt accgggatcg ccgaggcacg
     4801 ggccagaatc agtgtatgcg aggtgcgccc ggttttctcc agaatcatac ccgcaagatt
     4861 tttcaaatca aggctcaaaa actggcttgg ggttaaatct tcagccacca gaatggtcgg
     4921 tttttccagc accagattgt tgcgcggctt cagttccggc caggtgatat gcagcaactg
     4981 ttcgctgata tcgcgaatgt cgctgacccg ctcgcgcaga taatcgctgg cagaagcaga
     5041 tagtttggcg caaacctgct ccatattgct gatgatcgcc gcccccagtc cctgatgctg
     5101 ttctgtcatc aggcgacgga tattgcctgc aaattcatca tcctgaatca gcgacaaatg
     5161 agcgctgagg atagttttgc tttcgccgtc acgctcacgc aattgctgat tcagttgctc
     5221 ggcaagcgtt gccaggctgt gctccagccg ggtggaatct tgcgcactgg cggggattgc
     5281 ccgataactg tcgaggctgt cgctttgtaa cagggtcagc gtacccacgc cgacgccgct
     5341 tgccagcaca ttgccgtaca gtaaatccgg gttaaggcgg cttaatgaac gcggcagcgg
     5401 atgcgccgtc agttctgcct gcgtcggctg aacgctgtcg ctgtcgataa agcgcaccag
     5461 gatgtactct tccagcacgc gccgcgcctg ctcttcatcg ctgccgctaa tgttcaggct
     5521 gcaactgtca ttaaataagg tgccagttcc aatcaacgcc agcgagcttt tagcatcagc
     5581 ctttgcgttc tggcgatggt tgataaaggt gatttcgctt tgccactggc tgcactgttc
     5641 tttaagttcc cacgccggac gagcatgtag accgttaggc aaaggacaga gaaattgaat
     5701 cgttaacata accgctccgg gttaatcagg agatcatctg aaatccgtca gttgaacaac
     5761 cgtctcacgc gtaagacgtt gaataagggc agataaaagt tgctccatct gcaaaatgtc
     5821 gcggcaatcg gcaatcggca atcgatgccg cgcaatgtcc gtggcgggtg gcaggcccca
     5881 ttaccaatgt gggtacgcca gtaccggtta agtgcaccgc tccaccgtcc gtgccgccgt
     5941 tactgaacat atccgcctgc aacggaacgc caatttctgc cgccacggtt tcaatccacg
     6001 cggtaagttt tggcggcgca atcagcgatt tatcgcttaa caccagcatc ggcccgttac
     6061 caatctgacg atggttagcc gcgccataat caaagttttt cgcccagcag gcggtatcaa
     6121 gcacaatggc gacgtccggc gacaccgcgc gggtagcagt ttgcccgccg cgtaatccca
     6181 cctcttcgct ggaacttgcc accagccaca cttccgcagg cagttcggcg tcgtgcagct
     6241 cacgtagtaa cgtcaccagc agatagcaac tgaggcgatc atcaaaggct ttgcccatca
     6301 cccgctggtg agggaggacc tgaaaagtgg tatcgaaggt gacgcgatcg cctggacgaa
     6361 ttcccgcctg catcacttcg tcataggtgc gcgcgccaat gtccacgcgc atggcgctaa
     6421 cgtcattccc ctgccggtcg ccgtcaagca ggcctggaat tttgcactct tcacgggtgg
     6481 tgatgcgcac cggctgcagc tggcgggcag ccatgcgcac gttgccaacc ggcagcacgt
     6541 caatagcgcc ttcgcgggag atgctgcgca ccataaatcc cacttcgtcc atatgggcgc
     6601 agatcatcac cttcggacct gtcgattcat tgaggcggat cagcaccgat cccagaccat
     6661 caaatcgcac ttctttttgc aggcgagccg cttcttccag caggatctgc cgcacttcct
     6721 gttccgagga ggcgatcgca tctgcctcgc tcaacgcttt taatagcgat aaatccatta
     6781 tgcctctccc gtgagcaaca ctgttttcgg catggcgtag agcacttctg cgccttgcgg
     6841 ggtaaccagc acaacatctt cgatgcgcac gcccccttgc cctagcaaat aaatccccgg
     6901 ctccacggtc agcaacatgc ctggctgtag cgtcgtggtg tcccgcggtg aaaaacgcgg
     6961 atcttcatgg acttcaatgc cgatagcgtg accagtgtta tgaccgaaat agtcgccaaa
     7021 tccagcctcg gtaatgaccc ggcgcgcggc gtcgtcaatc tgctggcagc gcacgccggg
     7081 gcgaattgca gagattgccg cgagctgtgc ctgcaggaca atctgataga cgtcaaacag
     7141 cgggtgagat tcggcgctca ccccttcgcc attcaccagc aaggtgcgcg tcatatcagc
     7201 agtagccctg atacagcgca ccaaaatcga gagtgacaaa ctcgcccgct gcaacaatct
     7261 tgtcgctggc tttgccgtgc ggcagcgccc cgcgccagcc gctggcgaca atggtgtcaa
     7321 aagaggcttt ttctgcgccc tgctggcgca taaaccactc cagttcagcg gctatctcgc
     7381 gctcgctcat ccccgcctga ataaagcggc gaatatgctc cgctccgcga tcggcaatcc
     7441 cacaggcgag gcggattatc tccacctcct ctggcgtttt gatttgccgc agcacatccg
     7501 gcgtggcgct aaccagtttc gcattgagtt cagacttcca gcggtgcgcg gtttcccaac
     7561 tcacctgctg gccttcaaaa ccgagcgttt gcaactgctc atcggcaatg atttgattga
     7621 cgatagtggt aagcgtgttc gttgcgtcaa gcaaatgcag ctggtagcct tgcgcacggg
     7681 cttccacctc aacgaaatag cgcgaatcta ccagaatgtg cgcactttca cggctaataa
     7741 ccacatagcc tgatccggtg gagatcccca gatgcggctg tttgttctgc cgtgaggaga
     7801 gaagcaccgc atccagttgt tgcaccttaa gccagttgcg cagcgaagcg agtaatgtca
     7861 tctggatttt ccttcttaca ggctatcgat taacaatttg cctttgcgga acatcatcag
     7921 acgcaggaac accaccatca gcgcagtaat gattgccccc agcgcgatcc ccgccatata
     7981 aacgcccaag ttagtgacca gcggccatgc ccagatagcg gattccggga accattgcac
     8041 tgcgcccagc cagacagctg cggttgagcc gacaatcgcg ccgaccatat atgacggaat
     8101 agcggtgatg gggctttcca gcgcaaacgg aatcgcaccc tcactaatcc ccataaaggc
     8161 caggaacatc gcggtcttac cctgcggata aagttgagcg ttgaacaggc gtttgccggt
     8221 taaacgacgg tcaataatgg tcgccagacc cagaccaatc ggcggaataa cgatagcgat
     8281 agaacgagcg gtgaccggta atacgtggtc agtggtaaag ctgaaggcaa cgaaacctgc
     8341 ggctttgttg atcgggccac caaggtcgat tgctgtcgcc gcggcgatcc ccattgcgta
     8401 catcagcgcg cccttctcac ctgcggcagt cagcacagta cggataccgc cgttgatcca
     8461 gccaccaaag ggagtgatga cgtagtacat cgccagcatg acaaaaatgg cggaaagaat
     8521 cggtagcaaa aatgtggttt tgaacgctaa caggaaatcc ggtaactgga ttttctggtt
     8581 catccatttc acaaggtaac cggcaacgat agaaataatc agcgcaccaa tgaaggtgga
     8641 cggcaccggc gaagaggtcg cccattgcat agtgctggga tcgaagttca gcagttgcgt
     8701 cggctgggta gacatcaacc cgccgataaa accagccggg aacgccagtt tgccaccgat
     8761 agagttcgcc acaaatgcag caaacatcgg aatggcaaaa ccaaacaaca cgccgccgaa
     8821 ggactgcgac agccaggcaa atttaagtaa ggagaggtcg aaaccggaga atttcccgct
     8881 attaagagca tccatgatgc cgatctcagc aggaatttta agccagctat aagcaatcag
     8941 ctggctgaaa gcgaggatca caccgcccat aattaaggtc ggcaccatgc gtgaaatccc
     9001 tgacatcaca tgctgcggga gttcacccca gaagctgctt ttagaggcct gcggattctt
     9061 aactgccgct gccgcaccgg atgcgccagg cacaacggtt gcactgcgtt ttttaatggc
     9121 cataacaatt atccctgtcg aatattactg ttgttcagag gcaatcatct cttcgatttc
     9181 tttgatgatg cccgcagcgt ttttaattgc gtcctgcaaa gtgatttcat aaacgtcgcg
     9241 tgattcgaaa cgttcgttat cttccggggt aactgccacg gaatggatga tgatggtcgc
     9301 ttcggcgata tcctgcgccg tcaggcgatt ctggatgccg tccgcgccct gggtttcaat
     9361 tttcacttca taaccggctt ctaccgccgc ttcttccagc acctgagcgg ccataaaagt
     9421 gtgagccagg cccattgggc aggcacataa ggcaatcagt ttcttactca tcgttaattc
     9481 ctcgttcagt gatagttgac tagtgaaatt atgaacgtgc aaaacaaatc gccgttacca
     9541 gacaaataat gcatttactg gagaattaat cccgctgtac ggaagtgtga cgcaggtcgg
     9601 ccttgtgagg ggacacaaag aaaaattgaa ttaactgtat gatttaaaag attatcggga
     9661 gagttacctc ccgatataac aggaaggatt tacagaatgt gacctaaggt ctggcgtaaa
     9721 tgtgcaccgg aaccgagaag acccggatag tcatggacga tgagatacac cggaatatca
     9781 tggacatatt ctttaaagcg ccctttatct tcaaatgcgg cacggaaacc ggaggctttg
     9841 aagaactcaa ggaagcgcgg cacgataccg cccgcaataa acacgccgcc aaatgtcccg
     9901 agattgagcg ccagattgcc gccaaaacgg cccataatga cgcaaaacag cgacaatgcg
     9961 cggcggcaat cggtgcagct gtcagccagc gcgcgttcgg taatatcttt tggcttgaga
    10021 ttttctggca ggcggttgtc agctttcaca attgcgcgat acaaattcac cagcccaggg
    10081 ccagaaagca cgcgctccgc cgaaacatga ccaatttccg cacgcaatat ttcgaggata
    10141 atggcctctt cttcactatt cggcgcaaaa tcaacgtgac cgccttcgcc cggcaagctt
    10201 acccaacgct tatcgacatg gaccagatgc gcaaccccaa gccccgttcc ggcaccgtaa
    10261 accgcaatag gcttaccttc gaccggttct gcgccaccaa actgaatcag atgctctttt
    10321 ttcagcatcg ggatcgccat cgatacagcg gtaaaatcgt taataatttc cagatggcta
    10381 aaaccgagat tctttttcat ttcggcaatt gagaacgccc aggtatggtt ggtcattgcc
    10441 acccagtcac cggtaattgg gcaagcgatg gcaatacagc cgtctttcac ctcgacctta
    10501 tgttcttcaa gataaacgcg aatgaccgct tcgaggctgg ggtaatcaag ccctgaatag
    10561 gtcttagcct gcgagatttc accactggca atatcacaca gagcaagacg tgcgttggtg
    10621 ccgcccacat caccgactaa tgcatacttt gtcattcttc aactgctccg ctaaagtcaa
    10681 aataattctt tctcacactg taaatacctg ggggcataac aacaacgccg gaaaggcagg
    10741 ctccctgtaa atatcgatct gggtcacaca attactttat cgtttcagca ccaattgcag
    10801 cgatgccttt ttgcaagctg ggcaaactaa gtatctgacc ccgcataagg aatagaacat
    10861 gctccatccg cgagccagaa ccatgttgtt attatcgctc cccgccgtgg caattgggat
    10921 tgcgtccagt cttattctga ttgtggtgat gaaaatcgcc tcggtattac agaatttgct
    10981 ctggcaacga ctgccgggaa ctctggggat agcccaggat tcacccctct ggatcatcgg
    11041 tgtattaacg ctaacgggta ttgcggtggg gttggttatc cgtttcagcc agggtcatgc
    11101 cggaccagac cccgcctgtg aaccgctgat cggcgcaccg gttccgccct ctgcgctacc
    11161 tagacttatc gtagcattaa ttctcggtct tgctggcggc gtcagcctgg ggccggaaca
    11221 tccgatcatg accgtcaata tcgcccttgc cgtcgccatt ggcgctcgtc tgttaccgcg
    11281 cgtcaaccga atggagtgga ctattttagc ctctgccgga accatcggcg cgctgtttgg
    11341 tacacctgtt gcggcggcgt tgatattttc gcaaacctta aatggcagta gtgaagttcc
    11401 gctatgggat cgtctttttg cgccgttaat ggcggcagca gctggtgcac ttactaccgg
    11461 attatttttc catccccatt tttcactgcc cattgcgcat tacggacaga tggaaatgac
    11521 cgatattctc agcggtgcaa ttgtcgcggc gattgccatc gcagcaggga tggtcgccgt
    11581 atggtgctta ccacggttgc acgcgatgat gaatcaaatg aaaaatccgg tgctcgtgct
    11641 gggtattggc ggatttattc tcggaattct gggggttatt ggtggaccag tttcgctgtt
    11701 taaagggctg gatgagatgc agcagatggt ggcaaatcag gctttcagca ccagcgatta
    11761 ctttttgctg gcggtaatta aacttgccgc cctggtcgtt gctgccgcca gtggctttcg
    11821 cggtgggcga atcttcccgg cagtgtttgt cggcgtggca ttagggttga tgctgcatga
    11881 gcacgttccc gccgttccag cggcaataac cgtttcctgc gctattctcg gcatcgtgct
    11941 ggtggtaaca cgcgatggct ggttaagtct ttttatggcg gcagtcgttg tacccaatac
    12001 cacattgcta ccgctgctct gtatcgtcat gcttccggca tggctgttat tagcaggtaa
    12061 gccgatgatg atggtcaatc gtccgaagca acagccaccc cacgataatg tttagcaaaa
    12121 aatgctctcc tttatgattt aagagttatg gcgatgatac ggggctttac ctccccgtaa
    12181 tattgcgtta acaggccgct gacggcttcg atcgtgaagg agaataacaa tgttcaggtc
    12241 actgtttctg gcggccgccc tgatggcatt taccccgctt gcagcaaatg caggtgaaat
    12301 caccctactg ccatcgatca aattacaaat tggcgatcgc gatcattacg gtaattactg
    12361 ggacggtggt cactggcgag atcgtgatta ctggcatcgc aattatgaat ggcgcaaaaa
    12421 ccgctggtgg cgtcatgata atggctatca tcgtggctgg gataagcgta aagcatatga
    12481 gcgtggctat cgtgaaggct ggcgcgatcg tgacgatcat cgcggaaaag gccgcggaca
    12541 tgggcaccgc cattaaacgc gtcctccaat ggagcacaat gcctgatgcg acgctagcgc
    12601 gtcttatcag gcctacaaac cggctcttta acgcgtaggg cggataaggc gtttacgccg
    12661 catccgccaa tagtgccaga tgcgacgctc attcaactac aatcccagcg ccgtccccac
    12721 caacaaccag atattcagcg ccacgaccag cactacaatc acccagcctg tctgttttac
    12781 gcgtttgctg ttcaccagat cgcccatcaa cttgctgtca ctggtgaaaa tcagcagtgg
    12841 aaccagcgcc agggcgatac caaaacttaa cagcacctga ctcataacca gaatccgtgt
    12901 cggatctaat cccatcagaa tgacaataaa tgacggcaac atggtgactg tacgacgcac
    12961 ccacagcggg atatggaagc gaatgaatcc ctgcatcacc acctgccccg ccagtgtccc
    13021 caccaccgtt gaggacagtc cggcagcaac cagacttaac ccaaagaccg ttgccgcagc
    13081 atggcttaac agcggttgca gcgtcagata agcctcatca agatcggcaa caccagtatg
    13141 accagaaaag tggaacgccg ccgcagctgt agccatcatc accagattga caaaaccggc
    13201 aatcgtcatg gcgatagcca catcccattt ggtggcggaa taacgttgtt gacgcgaacc
    13261 gccatgtaaa tgctgagtga gcgaagagtg caaataaatc acatgcggca taatcgtcgc
    13321 ccctaacacg cctgctgcca ggaagaccgc ctccgaagta ggtaaactcg ggatcaccat
    13381 tcctttaccc agctgcgcca ggttaggctg ggagaaaatc aactcgacaa tgtaagccgc
    13441 ggcaacaaac aacagtaacc cgccaatcac tttctccagc ggtttttgcc cgcgacgttg
    13501 cagcattaaa atcaggaaag tcgcgatccc cgtcagcacc gcgccctgca acaacgaaac
    13561 accaagaatg agtttaaaac cgatcgccgc accaataaat tccgccaggt cggttgccat
    13621 cgcaataatt tctgcctgaa cccaatagaa ccacactacg ggacgcggat agtgatcacg
    13681 aatctgctcc gccagatttt taccggtggc aatccctagt ttggcagaga ggatctgaat
    13741 cagcatcgcc atcaggttgg cccaaacgac aacccacagt agctgatagc cgaagctagc
    13801 acccgcctga atattggtcg caaagttacc gggatcgata taaccaatcg ccgcaatgaa
    13861 cgcaggtccc attaatgcga gcctcatctt acgcgccgcc cgtccgctgc tactctcaac
    13921 gcgatagttc gtcatcttgt gcctctaaaa catagccttt gctatgtttc atgctatgct
    13981 aaacgagaat gattatcaaa ttcatttaaa tggattgtgg tgatttctct gatagaccaa
    14041 gattatgatt acgaaaagat tgatgacttg aatgttatgg ttatgtttaa tgttagcaca
    14101 tttacataac tttcagcttc catacacaac atagcagaaa tgtatgacag atcactattt
    14161 ttgaagcctg tcacaggacg tcattatagt gtgtgtcaga tctcgttttc cttaaccatg
    14221 ttacatagaa tgtgcacgga aatttaacct gcctcatatt tggagcaaat atggaccgcg
    14281 tccttcattt tgtactggca cttgccgttg ttgtgattct cgcactgctg gtaagcagcg
    14341 accgcaaaaa aattcgtatc cgttatgtta ttcaactgct tgttatcgaa gtgttactgg
    14401 cgtggttctt cctgaactcc gacgttggtt taggcttcgt gaaaggcttc tccgaaatgt
    14461 tcgaaaaact gctcggattt gccaacgaag ggactaactt cgtctttggt agcatgaatg
    14521 atcaaggcct ggcattcttc ttcctgaaag tgctgtgccc aatcgtcttt atctctgcac
    14581 tgatcggtat tctccagcac attcgcgtgt tgccggtgat tatccgcgca attggtttcc
    14641 tgctctccaa agtcaacggt atgggcaaac tggaatcctt taacgccgtc agctccctga
    14701 ttctgggtca gtcagaaaac tttattgcct ataaagatat cctcggcaaa atctcccgta
    14761 atcgtatgta caccatggct gccacggcaa tgtccaccgt gtcgatgtcc atcgttggtg
    14821 catacatgac catgctggaa ccgaaatacg tcgttgctgc gctggtactg aacatgttca
    14881 gcacctttat cgtgctgtcg ctgatcaatc cttaccgtgt tgatgccagt gaagaaaaca
    14941 tccagatgtc caacctgcac gaaggtcaga gcttcttcga aatgctgggt gaatacattc
    15001 tggcaggttt caaagttgcc attatcgttg ccgcgatgct gatcggcttt atcgccctga
    15061 tcgccgcgct gaacgcactg tttgccaccg ttactggctg gtttggctac agcatctcct
    15121 tccagggcat cctgggctac atcttctatc cgattgcatg ggtgatgggt gttccttcca
    15181 gtgaagcact gcaagtgggc agtatcatgg cgaccaaact ggtttccaac gagttcgttg
    15241 cgatgatgga tctgcagaaa attgcttcca cgctctctcc gcgtgctgaa ggcatcatct
    15301 ctgtgttcct agtttccttc gctaacttct cttcaatcgg gattatcgca ggtgcagtta
    15361 aaggcctgaa tgaagagcaa ggtaacgtgg tttctcgctt cggtctgaaa ctggtttacg
    15421 gctctaccct ggtgagtgtg ctgtctgcgt caatcgcagc actggtgctg taaaaccata
    15481 cataaaaaag ccggggatca ttccccggct tttttacgcc tgttaatcag ctaatggctg
    15541 cgggcgacca atcaaatacc cttgcagata ttgcaccccg agcttatgca atagcgcctg
    15601 ctgctgttgc gtctcgacaa actccgcgac cacactcaat gacttcgctt tcgccagatc
    15661 ggtaattgat ctcacaatca tcgcatccag cgtattcgtg acaatatctt tcacaaagac
    15721 gccatcaatt ttgatgatat cagcctgcaa acgctttaac cgttcgtagt tggcatatcc
    15781 ggtgccaaag tcatcaatcg caatccggaa accaaactta tgcagctgct cgatgttgta
    15841 catgctgctt tctgcgttag aaaacgcctg ctcctcggtg atctcaagaa tgaccgcctg
    15901 cggggagatg tgataacgct taaacagacg aataatccgc ccggcaatat tcttttgcag
    15961 cagcgtgagc ggcattaaat tgactgaaaa gcgcggtcct tttttgtcgc aagggtgtgt
    16021 tgccagccac ttcaacaagg attccagcac ttgcaaatca aaacgcgcgc taaggttaaa
    16081 ctgagcaata aggggcagaa acttatccgg ggtcataatg ccgccgtcat atttcagtcg
    16141 cgcgaggatc tcatcataac cttcaccctc tttgttgcga attggctggg cgtagagcag
    16201 caaatcaccc tgatctaacg ccgtgcgaat ggtattcagc aatagcacct gtttagtggt
    16261 ctgcccggaa accatctcct ctctgctatc cagcgccagc acatgatgat gtgcgcagga
    16321 ttgctccgcc agccagctta actgccccaa caagggttgc agggtttcct gatttccatc
    16381 aaaacgcccc caggcagcac catagcccat atccagcccg gtattgttcc agtgaatttg
    16441 ccgactattc aggatgttaa ccatatgctg gagtcgccct tccgtttccg gcccgctcag
    16501 cactaacagc agttcactac ccggcaattg atacaacttt tcgttttcct gcatcaacgg
    16561 cagcagcgta cggcaaattg agcggataca gtgaacgcgc atcattaagc cgtaatgacg
    16621 actcataaac tcaagattat caatgcgcag gcagcaaaaa ctcttgcccg cctcttgctc
    16681 cggcgcttgt tccaacgcac gaaagttggg taggagcgtt aacggatctg ttagcgcctg
    16741 caaatgccag cggcgattaa gccattcact gcgatgataa atgcgcacca tatagagcag
    16801 gcaaacgcta aaggaaatca gcaccgcgag aataaatgcc agcgaatatt cggtttccac
    16861 cccttgcaaa aagttctgat tgtaattcag aaggcaaagc gttgaaacag cccaggtgag
    16921 atttaaaaac ggatagcgaa gattaccgac cccgagggta aagatgatga agaaaacggg
    16981 taccaggtaa ccggcaataa agtcgttttc ataaggtgtg cacaacagaa gtagcagcac
    17041 gcttaacgct gccagccagc ttaaggtaaa tgcgagtgag atagtagaaa agcatgttgt
    17101 aaatcagcac tgcggtgaaa aggcttagta aatcaacgac cgtgaaaatg gcatccgcat
    17161 cgccgaaaaa ggtggatatc tttagcggga aatcaaagaa gctccccaca agatacatgc
    17221 tgcatttgat gccgatcggc gtcaccaaac caagccagaa cagacgttgc cagacatatc
    17281 ggctggtcaa tccataacgc caacgcgtcc ccagctgcca gcgtaatatt gcgcaggcac
    17341 acaacacgac aaacgtctgg ctgccgagca tgaccgcagt ctgtaaaaaa gagagatgaa
    17401 agttccacag attggtgcaa aacatcccgc ataaaacggg aacgacaccg cgccagccaa
    17461 agataaacaa gattgacagc attacgcaca gtggcatcca cgccagaaag atataactgg
    17521 aatggataat ggcaagtggc gaaataaaac gggatagctg aataagtacc acggtgagcg
    17581 taaacgctag tgtgaatatc ttgatatttt ttatcaggtt atgctccaca aacatgagta
    17641 cactactctc actgatcggg gttttcaggc acacaaataa taagtttatt cgcatcgcga
    17701 agcaaacagg ggccagcctg ttgccgattt ttcatccaat gatgctttca ccaggcggcg
    17761 ctaacccgga cgtagactgt attacaaaag cggcaaaaag cagagacaaa aaacccccgc
    17821 tttgcagcga gggttggaaa tttggtggag ctaagcggga tcgaaccgct gacctcttgc
    17881 atgccatgca agcgctctcc cagctgagct atagccccac gatgcgttta cgtaccaaat
    17941 ttgctgggtg caaaatttgg tggagctaag cgggatcgaa ccgctgacct cttgcatgcc
    18001 atgcaagcgc tctcccagct gagctatagc cccgtcacgt aaagcttgtc gagttgacgg
    18061 gcggcatcat atgaattccg cccgaatgtg tcaacggcaa attgcaacgt gtagtttcaa
    18121 tcgctgaaaa atcaggcaaa tgaacaattt tggaaaaccg ctcgcattca gtagttattc
    18181 atgtcacggt ttcctgtaaa gtggtgttat aaaatgaact actaatagac ccacatacat
    18241 tcagggaatt gttatgttca aggagaggat gacaccagat gaacttgcca gactgaccgg
    18301 ctatagccgc cagaccatta ataaatgggt acgcaaggaa ggctggacaa cgtcaccaaa
    18361 acctggcgtc cagggtggca aagcccgact ggttcacgtc aatgaacagg ttcgtgaata
    18421 tattcgcaat gctgaacgtc cagaaggtca gggagaagcg cctgcccttt ctggtgatgc
    18481 gccgcttgaa gtattgctgg tgacactggc gaaagagatg acgccagttg aacaaaaaca
    18541 gtttacatcc ttgcttctgc gggaagggat tatcggattg ttacaacgct tagggattcg
    18601 cgatagcaaa taatatgaaa agattacgca ataaaatgac caccgaagaa ctggctgaat
    18661 gcctcggtgt ggctaaacaa actgttaacc gttggaccag agaaaaaggc tggaaaacgg
    18721 aaaaatttcc cggcgtgaaa ggcggtcgtg ccagacttat tctggtcgat acgcaagttt
    18781 gcgagtttat tcagaacacg ccagccttcc ataacacacc aatgttgatg gaagctgaag
    18841 agagaattgc tgaatacgct cctggagcac gcgccccggc gtatcgacaa atcatcaacg
    18901 cgatagataa tatgacagac attgagcagg aaaaagtcgc gcaatttttg tcacgtgaag
    18961 gaattcgcaa tttcctcgcc cgtctcgata ttgacgaatc agcataaata aaaaacggca
    19021 ggatattatc tcctgccgtt tatcttttta cacgctaatt actgctgatt ttcgcgttca
    19081 gcaataaaat ccagcgcttt gttgatacgc tcgatactgc gggtcttacc aattgcgtga
    19141 acggtaacat ccagtgctgg agactgcccc gcaccggtta cggcgacacg cagcggcata
    19201 ccaactttac ccatacccac ttccagctca tccgccgtcg cctgaatagc gtgatgaacg
    19261 ttttcagcgg tccagtcagt aatcgcggcc agtttgtcac gaaccacttc cagcggctga
    19321 cgcgctaccg gacgcagatg ttttttcgcc gcatcggcat cgaactcagc aaaatcttcg
    19381 tagaaataac ggcagctctg tgccatctct ttcagcgtct tgcagcgttc gcccagcagt
    19441 ttcaccagat cagccagctg cgggccgtta cgggtatcga tattttcctg ctcaatgtgc
    19501 cactgtaagt gagtagcaac atactccggc ggcagcgcgt taatgtagtg atggttcagc
    19561 cacagcagct tgtcggtgtt gaacgcactg gcagatttgc tgacggcatt caaagtgaag
    19621 tatttgatca tctcttcacg agtgaagatt tcctgatcgc cgtgggacca gcccagacgc
    19681 accagatagt tcagcagtgc ttctggcaaa taaccgtcat cacgatactg cattacgctg
    19741 actgccccgt gacgtttgga cagtttttta ccgtcatcgc cattgatcat agaaacgtgc
    19801 gcgtaaaccg gcaccggcgc tttcagggcc ttaagaatgt tgatctggcg cggcgtgttg
    19861 ttgatatggt cttcgccacg gataacgtgg gtgatttcca tatcccagtc atcgacaacc
    19921 acacagaagt tataggttgg ggaaccatcg gtacggcgga taataagatc gtccagttcc
    19981 tggttgctga actcgatcgg accacggatc tgatcgtcaa aaacaacaga accttcctgc
    20041 gggttagcaa aacgtacaac acacggttca tcatcagcat gatgctcatg gctgtggcgg
    20101 cagcgaccgt cataacgcgg cttctcacct ttcgccattt gctcttcgcg cagcgcttcc
    20161 aggcgctctt tagagcaata gcatttataa gcagtgccct cttccagcat ctgatcgatc
    20221 accgcgttgt agcgatcaaa acgtttggtc tggtagtacg gaccttcatc ccactccagg
    20281 ctcagccagt tcatgccatc cataatggct tcgatagctt ccggcgtgga acgctcaaga
    20341 tcggtgtctt caatacgcag cacgaactca ccgccgtggt tacgtgcaaa aagccaggag
    20401 taaagagcag tacgcgcgcc gccaacgtgc agatagcctg ttgggcttgg cgcgaagcga
    20461 gttttgattt tcatgaaatg gccttacgtt tagaaagatg ccgacaaccg gcaaatcctg
    20521 gaaaaattga gtgggcgata ttctatcact cccgcctgat tcctcaatgt agttcgggtt
    20581 tctaccttac gcattattgt tttcgttgac aaattgcgca acacggtctg ttttgcgatc
    20641 atcttgctta aatttacgac gaacgaacaa tttctttaga aaatgcgttg actcattttg
    20701 aactctccct ataatgcgac tccacacagc gggggtgatt agctcagctg ggagagcacc
    20761 tcccttacaa ggagggggtc ggcggttcga tcccgtcatc acccaccaac tactttatgt
    20821 agtctccgcc gtgtagcaag aaattgagaa gtgggtgatt agctcagctg ggagagcacc
    20881 tcccttacaa ggagggggtc ggcggttcga tcccgtcatc acccaccact tcgggtcgtt
    20941 agctcagttg gtagagcagt tgacttttaa tcaattggtc gcaggttcga atcctgcacg
    21001 acccaccaat gtaaaaaagc gccctaaagg cgcttttttg ctatctgcga tactcaaaga
    21061 ttcgaacctg cagcaggttt gagttgagcg cagcgaaaca acggagccgc tcgcggcgac
    21121 ggcccgaagg gcgagcgaag cgagtcatcc tgcacgaccc accaatgcaa aaaagcgccc
    21181 taaaggcgct tttttgctat ctgcgatttg taagattgat ttttcttctc aagttccttg
    21241 ttcttctacc gatattacct cgtacagtga cgaggactga acaatggcca ctatctcttc
    21301 aacttcaatt ccttccatac aaacacagag tagtaacagg gcatcacaag gtagcgatgt
    21361 tgcttcacaa atcgcgcgga taagtcagca aatcatcaaa ctcactcaac aaattaaaga
    21421 gattgtagac actagcggca gtgctgaaga caagcaaaaa caggccgaat tgattcaaca
    21481 gcaaatcacc ctactggaaa cacaactggc gcagttgcag aaacagcagg cagaaaaggc
    21541 gcaggagaaa gagcagcgtt tgtctctgaa tgtatccctt ctaaatcctg ttgaaaaaac
    21601 gactcatatc gatatctata tctgatacaa cggatcccct tcccgaccag ccattagcgc
    21661 attgccctgg tctcgtacca cgtcccataa cgcttcagcg gcggtggaaa gtgaacggtt
    21721 cttcctgcgc accagcatca actggcgttc aaccaccggc gtaatgcgct taaccaccag
    21781 tgggctacct tcaggcaatg gtagcgccag tgcaggaaga atactgatgc cgatgcctgc
    21841 ggctaccatc gggaacagcg tcgccggatg tccaatctcc tgcacaatat tcgcctgaat
    21901 accattgcgc gccagcgctg cgtcaatcag cggtcggctg cctgacgcgt aatcctgcaa
    21961 caccaatttc gctccctgta atgcctgcca gggtacataa tcttccacgg ccaaagcgct
    22021 atcgcgatgg caaagcagaa agaaaggctc ggaaagaatc gcttcacatt gcagatcgcc
    22081 cactgggcct ggatcgatga cgatgccaaa gtcgacatct ccctggcgaa tactttccat
    22141 cacccactgc tgcggacgat catgcaggac aaactgaata tctggatagc ggcgatggct
    22201 ttcagcaatg cattgcggaa taagatgcgc ggaaatggtc tggctggcag cgacccgaac
    22261 ttttccgctc agttgttgcc ccatacgccc ggtatcgcgc aacgtgctgt taagttcatc
    22321 cagcagtcgc tcaagacgca aagccaactg ctgccctgca tctgtaagca ccacttcacg
    22381 cgtggttctg tccagcaggc gaacaccagt atgattttcc agctccttca cactgtgact
    22441 tactgccgac tggctcaagc cgatacgctc tcctgcacga ctaaaacttt tctcctgcgc
    22501 tactgtgacg aaaaccttta attgttttaa agaataattc atctgttttc ttcatgaatg
    22561 gatgcaataa atcaatttta tttctcaaac tggaagaagc acaatagaac catcgatcat
    22621 ctggagtctt tatgaaactt tttcgtatcc tcgatccttt caccttaacc ctgatcacgg
    22681 tggtgttgct ggcctctttc tttccggcca gaggcgattt cgtccccttc tttgaaaatc
    22741 tgaccaccgc agctattgcc ctgctgttct ttatgcacgg cgcgaagttg tcgcgtgagg
    22801 cgattattgc tggcggtggt cactggcgac tgcatttgtg ggtaatgtgc agcaccttcg
    22861 tgctgtttcc gattctgggt gtactgtttg cctggtggaa accggtaaat gtcgacccga
    22921 tgctctactc cggttttctc tacttgtgca ttctcccggc taccgtgcag tctgcaatcg
    22981 ccttcacgtc aatggcgggc ggtaacgtcg cggcggcggt ttgttctgcg tcagcatcca
    23041 gcctgctggg gattttcctt tcaccattgc tggttggtct ggtgatgaat gttcacggtg
    23101 caaggggcag ccttgagcag gtcggtaaaa ttatgctgca actgctgctg ccgtttgtgt
    23161 tggggcatct ttcccggccg tggattggtg actgggtgtc gcgcaataaa aaatggattg
    23221 cgaaaactga ccagacgtcc attctgttgg tggtttatac agcgttcagc gaagccgtcg
    23281 ttaatggtat ctggcataaa gttggctggg gatcattgct gtttatcgtg gtggtcagct
    23341 gcgttcttct ggctatcgtg attgtagtta acgtctttat ggcacgccga ctgagcttca
    23401 ataaggcaga tgaaattact atcgtctttt gtggttcgaa aaagagtctg gcaaatggca
    23461 tcccgatggc aaacattctg ttccccacat cggtgatcgg tatgatggtg ctgcccctga
    23521 tgattttcca tcagatccaa ttgatggtct gtgcggtgct ggcgcgtcga tacaaacgcc
    23581 agaccgaaca gttacaggcg cagcaggaaa gcagcgccga taaagcttaa agcggacgct
    23641 tcaggggctg gaccagttgc gtcagcccct cggttttaat cagcagcgtg atttgcatta
    23701 gctcaccgag cttccctgcc gggaactcat ctttgcgggc aaaccacagc agatactcct
    23761 ccggcaggtc aattaagcga cgccctttgt atttgccaaa cggcattatc gtattggcta
    23821 tttcaatcag ctgctctttt tccatctcag ctacccagca aacgcagcat ttccgcttcg
    23881 tcgatgactt caatgcccag ttcctgcgcc ttcgccagtt tagatcctgc agcttcaccc
    23941 gctatcacca gatcggtttt cttcgacacg ctgcccgcga ctttcgcgcc cagttcgacc
    24001 agtcgagctt tagcgtcatc acgcgacatc tggcttaagc tgcccgtaag caccacggtt
    24061 ttaccagcaa acgggctgtc aatctcttcc gcgttgataa cgatcggcgc aggccagtga
    24121 acaccttccg ccaacagctc gctgatgaca ttgcggttgc tttcttcggc aaagaagttg
    24181 tgaacgtggg atgcaacgac aatgccaaca tcaggcacct tttgcagctc ttcaatcgaa
    24241 gcggcttcca gcgcttccag cgtgccgaaa tatgccgcca gacctgctgc ggtggcctcg
    24301 ccgacttcac ggatgccaag tgcatagagg aagcgagcaa aggtggtttc tttcgctttt
    24361 tccagcgcgt taaccacgtt ttgtgccaat tttggcccca tacgctccag tccggtcagt
    24421 ttgcctgcgg tgagtttgaa cagatctgcc ggagtatgga catattcttt ttcaaccagc
    24481 tgatcgatga ttttgtcgcc cattccgtca acatccatcg cacggcggga aacaaagtgt
    24541 ttcagcgact ctttacgctg cgcaccgcaa atcaggccac cggtacagcg ggcaaccgct
    24601 tcaccttcca cacgctcaac gtcagaacca cataccggac aatgcgtcgg gaatacaacc
    24661 tcacgggtat cttccgggcg ttcagaaagc acgacgttaa ccacctgcgg gatcacgtcg
    24721 ccagcgcggc gaatcaccac tttatcgcca atgcgtaaac caagacgttc gatttcatcc
    24781 gcattgtgta aggttgcgtt actcaccagc acgccagcaa catggacagg ttccagacgc
    24841 gcaacaggcg taatcgcgcc agtacgccca acctgaaact cgacgtcacg cacaaaggtc
    24901 atctgctcct gcgccgggaa tttaaacgca accgcccagc gcggggcacg cgcgacaaag
    24961 ccaagctgct cctgctgttc cagtgagttg accttaatca ccacgccgtc gatatcaaag
    25021 cccagcgtcg ggcggtcttc ttccactttg tgatagaacg ccagcacttc ttccgccgat
    25081 tcacaaagcg ttacccgatc gctgaccggc accccccact ttttaaattg cagtaaacgg
    25141 ccaagatgag tatccggcag ctcgccacct tccagaacac caacgccata gcagaaaaaa
    25201 gtgagcggtc gcttcgctgt aatacgcgga tcaagctgac gcagtgaacc agctgccgca
    25261 ttacgtgggt tagcaaaaac tttcccgccc gtgcgtcgcg catcttcgtt aatcttttcg
    25321 aaccccgcct gcggcaggaa cacttcacca cgcacttcca gacgcgccgg gatattctct
    25381 ccgtgcagct tcagcggaat ggcgcgaata gtacgcacat tagacgtgat atcttccccg
    25441 gtggtgccat cgccacgggt cgcggcactg actaaaacgc cattttcata cagaatactg
    25501 acggcaaggc catccagctt cagctcacag caccaggtga ctttctcgtt gtttttcaga
    25561 cggtcctgca cacgtttgtt gaaagcaaga aagctttctt catcaaaaac gttatccagt
    25621 gacagcattg gtacttcatg gcgtatctgg ctgaaagctg ccagcggcgc agcacctaca
    25681 cgttgggtag gcgaatcagg cgtaatcagt tctggatgtt tggtttccag ctcgcgcagt
    25741 tcgcgcatca gcctgtcgta ttcagcgtcg ggaatttccg gcgcatccat cacatgataa
    25801 agatattcat gatggcaaag cgtcgttcgc agttctgtca gttgttgttc gattgattcc
    25861 atatcgcacc atcaatgcta aaaacccccg acaagcgggg gttcgaagag gagttaattt
    25921 gccttaagtg tatcaggcgt tggcgtcttt gacttcgcgg atgatgtcct ggtactcgcg
    25981 caatttctgc ggagtcatca tacggcgctg atcgtcaagc acgacaccgc ccacttcatc
    26041 ggcaatatgc tgcgcagatt gcagcatcag cttgaagttc tgcagctcgt caccgtaaga
    26101 cggtacctgc ataaagatag tgacacccgg agtagtgaaa tccttcattt caggatcaaa
    26161 ggttcccggt ttcaccatat tcgccaggct gaataacgcc gggccgctgc catccgggct
    26221 aagatgacga tggtaaatat tcatatcgcc aaaaatgaag cccgcttgtt gaatgctgtt
    26281 aagaagcagt tcaccgttta gctcgctacc gtgatgcgcc gcgacgttca tgataatcac
    26341 cgcttctttg cgcttcggtt tatccataac tggagccggt tccgctacag gctcaggctg
    26401 tggtgccgct acgggttctg caggctggaa agcctgttgt gccggttgcg gtgctgaatg
    26461 cacaggctgc ggcgctggcg cgacctgtgg cgaaactggc tgctgcaacg gctgttcagg
    26521 ctgcggctga taggcaggct gctgcaccgg ctgcgctgga cgcggagcat gttgcggcgg
    26581 tacctgcgct tcaggcggct gctggaccgg ttgacgcggc tgcgcagacg cataaggcgg
    26641 taggtactgg tgttgcggcg acggacgagc agcctcatgc tcctgagcgt tagccggggc
    26701 atgattcacg cggtgaacac gaacctcacc aacgccctca tcatcttcga catcctcgtc
    26761 ataagaatcg tcgtcacgtt ttgacttcat tcgttttaat ggccgatcgc ggaacataga
    26821 agatcgttct ttacggctgg tccagaaacc atgtaccagt aaagcgatta tggcgatcgc
    26881 gccaacaatg attaatatca gacgcaaatc ctgcatcatt atattctctg ttgttctaac
    26941 accttgccac cacggcaaac atttactcac taagagtatt tgccgattac ctcaagtgca
    27001 agtgcactat taactttcac agcacaaaga tagatgaaat cgtgcttttt gctgtttttt
    27061 cgaacatatc ctaactgtcc attgcgcaat tacccggtaa aatacgcaga attttcctgg
    27121 gattggtcaa aaggagctca tcctgattat ggtttcatca ttcacatctg ccccacgcag
    27181 cggtttttac tattttgcgc aaggctggaa gctcgtctcg caacctggga ttcggcgttt
    27241 cgttatttta ccgctgctgg tcaatatttt gttgatgggg ggcgcattct ggtggctctt
    27301 tacacagctc gatgtctgga tcccgacttt catgagttac gttccggact ggctacaatg
    27361 gctgagttat ctgttgtggc ctctggctgt catctctgtg ctgttagtgt ttggctattt
    27421 cttctccacg attgctaact ggattgccgc tccgtttaac ggtttattgg ctgaacaact
    27481 ggaagcacga ttgactggcg ctacaccgcc agataccggg attttcggta tcatgaaaga
    27541 tgtgccgcga atcatgaaac gcgaatggca aaaatttgtc tggtatctgc cgcgcgcaat
    27601 tgtattacta attctttact tcatccctgg tattgggcaa accgtcgcgc cggtactgtg
    27661 gttcctgttt agcgcctgga tgttagccat ccagtattgc gattacccct tcgataacca
    27721 caaagtgccg tttaaagaga tgcgcaccgc cctgcgcaca cgcaaaatca ccaatatgca
    27781 gtttggtgct ttaaccagcc tgtttacgat gatcccgctg cttaatctgt tcatcatgcc
    27841 cgttgccgtt tgtggcgcga cggcgatgtg ggtcgattgc tatcgcgata aacacgcgat
    27901 gtggcggtaa caatctaccg gttattttgt aaaccgtttg tgtgaaacag gggtggctta
    27961 tgccgcccct tattccatct tgcatgtcat tatttccctt ctgtatatag atatgctaaa
    28021 tccttacttc cgcatattct ctgagcgggt atgctacctg ttgtatccca atttcataca
    28081 gttaaggaca ggccatgagt aagatttttg aagataactc gctgactatc ggtcacacgc
    28141 cgctggttcg cctgaatcgc atcggtaacg gacgcattct ggcgaaggtg gaatctcgta
    28201 accccagctt cagcgttaag tgccgtatcg gtgccaacat gatttgggat gccgaaaagc
    28261 gcggcgtgct gaaaccaggc gttgaactgg ttgaaccgac cagcggtaat accgggattg
    28321 cactggccta tgtagctgcc gctcgcggtt acaaactcac cctgaccatg ccagaaacca
    28381 tgagtattga acgccgcaag ctgctgaaag cgttaggtgc aaacctggtg ctgacggaag
    28441 gtgctaaagg catgaaaggc gcaatccaaa aagcagaaga aattgtcgcc agcaatccag
    28501 agaaatacct gctgctgcaa caattcagca atccggcaaa ccctgaaatt cacgaaaaaa
    28561 ccaccggtcc ggagatctgg gaagataccg acggtcaggt tgatgtattt attgctggcg
    28621 ttgggactgg cggtacgctg actggcgtca gccgctacat taaaggcacc aaaggcaaga
    28681 ccgatcttat ctctgtcgcc gttgagccaa ccgattctcc agttatcgcc caggtgctgg
    28741 caggtgaaga gattaaacct ggcccgcata aaattcaggg tattggcgct ggttttatcc
    28801 cggctaacct cgatctcaag ctggtcgata aagtcattgg catcaccaat gaagaagcca
    28861 tttctaccgc ccgtcgtcta atggaagaag aaggtattct tgcaggtatc tcttctggag
    28921 cagctgttgc cgcagcgttg aaactacaag aagatgaaag ctttaccaac aagaatattg
    28981 tggttattct accatcatcg ggtgagcgtt atttaagcac cgcattgttt gccgatctct
    29041 tcactgagaa agaattgcaa cagtaatgcc agcttgttaa aaatgcgtaa aaaagcacct
    29101 ttttaggtgc ttttttgtgg cctgcttcaa actttcgccc ctcctggcat tgattcagcc
    29161 tgtcggaact ggtatttaac cagactaatt attttgatgc gcgaaattaa tcgttacagg
    29221 aaaagccaaa gctgaatcga ttttatgatt tggttcaatt cttcctttag cggcataatg
    29281 tttaatgacg tacgaaacgt cagcggtcaa cacccgccag caatggactg tattgcgctc
    29341 ttcgtgcgtc gcgtctgtta aaaactggcg ctaacaatac aggctaaagt cgaaccgcca
    29401 ggctagactt tagttccaca acactaaacc tataagttgg ggaaatacaa tgttccagca
    29461 agaagttacc attaccgctc cgaacggtct gcacacccgc cctgctgccc agtttgtaaa
    29521 agaagctaag ggcttcactt ctgaaattac tgtgacttcc aacggcaaaa gcgccagcgc
    29581 gaaaagcctg tttaaactgc agactctggg cctgactcaa ggtaccgttg tgactatctc
    29641 cgcagaaggc gaagacgagc agaaagcggt tgaacatctg gttaaactga tggcggaact
    29701 cgagtaattt cccgggttct tttaaaaatc agtcacaagt aaggtagggt tatgatttca
    29761 ggcattttag catccccggg tatcgctttc ggtaaagctc tgcttctgaa agaagacgaa
    29821 attgtcattg accggaaaaa aatttctgcc gaccaggttg atcaggaagt tgaacgtttt
    29881 ctgagcggtc gtgccaaggc atcagcccag ctggaaacga tcaaaacgaa agctggtgaa
    29941 acgttcggtg aagaaaaaga agccatcttt gaagggcata ttatgctgct cgaagatgag
    30001 gagctggagc aggaaatcat agccctgatt aaagataaac acatgacagc tgacgcagct
    30061 gctcatgaag ttatcgaagg tcaggcttct gccctggaag agctggatga tgaatacctg
    30121 aaagaacgtg cggctgacgt acgtgatatc ggtaagcgcc tgctgcgcaa catcctgggc
    30181 ctgaagatta tcgacctgag cgccattcag gatgaagtca ttctggttgc cgctgacctg
    30241 acgccgtccg aaaccgcaca gctgaacctg aagaaggtgc tgggtttcat caccgacgcg
    30301 ggtggccgta cttcccacac ctctatcatg gcgcgttctc tggaactgcc tgctatcgtg
    30361 ggtaccggta gcgtcacctc tcaggtgaaa aatgacgact atctgattct ggatgccgta
    30421 aataatcagg tttacgtcaa tccaaccaac gaagttattg ataaaatgcg cgctgttcag
    30481 gagcaagtgg cttctgaaaa agcagagctt gctaaactga aagatctgcc agctattacg
    30541 ctggacggtc accaggtaga agtatgcgct aacattggta cggttcgtga cgttgaaggt
    30601 gcagagcgta acggcgctga aggcgttggt ctgtatcgta ctgagttcct gttcatggac
    30661 cgcgacgcgc tgcccactga agaagaacag tttgctgctt acaaagcagt ggctgaagcg
    30721 tgtggctcgc aggcggttat cgttcgtacc atggacatcg gcggcgacaa agagctgcca
    30781 tacatgaact tcccgaaaga agagaacccg ttcctcggct ggcgcgctat ccgtatcgcg
    30841 atggatcgta aagagatcct gcgcgatcag ctccgcgcta tcctgcgtgc ctcggctttc
    30901 ggtaaattgc gcattatgtt cccgatgatc atctctgttg aagaagtgcg tgcactgcgc
    30961 aaagagatcg aaatctacaa acaggaactg cgcgacgaag gtaaagcgtt tgacgagtca
    31021 attgaaatcg gcgtaatggt ggaaacaccg gctgccgcaa caattgcacg tcatttagcc
    31081 aaagaagttg atttctttag tatcggcacc aatgatttaa cgcagtacac tctggcagtt
    31141 gaccgtggta atgatatgat ttcacacctt taccagccaa tgtcaccgtc cgtgctgaac
    31201 ttgatcaagc aagttattga tgcttctcat gctgaaggca aatggactgg catgtgtggt
    31261 gagcttgctg gcgatgaacg tgctacactt ctgttgctgg ggatgggtct ggacgaattc
    31321 tctatgagcg ccatttctat cccgcgcatt aagaagatta tccgtaacac gaacttcgaa
    31381 gatgcgaagg tgttagcaga gcaggctctt gctcaaccga caacggacga gttaatgacg
    31441 ctggttaaca agttcattga agaaaaaaca atctgctaat ccacgagatg cggcccaatt
    31501 tactgcttag gagaagatca tgggtttgtt cgataaactg aaatctctgg tttccgacga
    31561 caaaaaggat accggaacta ttgagatcat tgctccgctc tctggcgaga tcgtcaatat
    31621 cgaagacgtg ccggatgtcg tttttgcgga aaaaatcgtt ggtgatggta ttgctatcaa
    31681 accaacgggt aacaaaatgg tcgcgcctgt agacggtacc attggtaaaa tctttgaaac
    31741 caatcacgcg ttctctatcg aatctgatag cggcgttgaa ctgttcgtcc acttcggtat
    31801 cgacaccgtt gaactgaaag gcgaaggctt caagcgtatt gctgaagaag gtcagcgcgt
    31861 gaaagttggc gatactgtca ttgaatttga tctgccgctg ctggaagaga aagccaagtc
    31921 taccctgact ccggttgtta tctccaacat ggacgaaatc aaagaactga tcaaactgtc
    31981 cggtagcgta accgtgggtg aaaccccggt tatccgcatc aagaagtaat tcttgccgca
    32041 gtgaaaaatg gcgcccatcg gcgccatttt ttatgcttcc gccagcggcg gcaaaatcaa
    32101 ttcatcgctc tcatgctgct gggtgtagcg catcacttcc agtacgcgca accccgcacg
    32161 gtgcaccgca tcgtttaacg ccttcccttt cagcaaacca ctgataagct gagcacaaaa
    32221 caggtcgcca gtccctttca ggtcggtttt tacccgtgaa tgggaaatga cattcacgct
    32281 gtcggcactg accaccacca cctgcatctc ctgattttct tcattaccgg aggcgctggt
    32341 aatcaccacc cattttaatg tgtctgaaag cagacttttt gcggcagcaa tggcactgtc
    32401 gagatcgcgg caatttttac cggtcaggat ttccagctca aagatattgg gggtaattcc
    32461 ctgcgccagc ggcagtaaat attgtcgata cgcctcggga aggtcaggtt tgacataaat
    32521 tccgctatca atatcgccaa tcaccggatc gaccatgatc aataggtcag gatggtcttt
    32581 gcgtagcgca gtcagccact cggcaaggat tttgatttgc gatgccgttc ccatatagcc
    32641 cgtggttaca gcacgaagtt ggcgcagcgc atcacgctcc tgaagcgcac gcaaatagcc
    32701 gctaaaccat tcgtccggaa tcgcaccacc gtagaaagtg tcataatgcg gcgtattgct
    32761 cagcaatacc gtcggcacgg caaagacatt caagccgttc tgttttatag caggcaccgc
    32821 aatgctgttg cctacgcttc cgtacactac ctgcgactgc acggcgacga tatccgcctg
    32881 cagcgcccta ctcttatcgt taaacaacaa caaactactc atttaatttt ttctccttgc
    32941 cgatgatcct catcgtattc caaccgaaac tttacctgat tctggcagtc aaatcggcta
    33001 ttacaaaaca aagataaggt aattcagtga agaaaatcat ttgtctggcc attacgctgc
    33061 taatgacact ccccgcttac gcgaagttaa ctgttcatga agaagcccgc atcaacgcca
    33121 tgctggaggg attagcacag aaaaaggatt tgatatttgt gcgcaacggt gatgaacata
    33181 cctgcgatga agcggtttcc cacctgcgtc tgaagctcgg caatacccgt aaccgcattg
    33241 acactgccga gcagtttatt gataaggtag cttcgtcgtc atcgattacc gggaagccgt
    33301 atatcgtgaa gatccccggt aagagcgatg agaacgcaca gcctttttta catgcgttaa
    33361 ttgcgcagac ggataaaacg gtgcctgcgg aaggaaatta atccgctttg gtaaggcatt
    33421 tacaggaggt aacatgaaaa aatgctttat ttatcacgat gaaaaatcga ataaattttg
    33481 gtggatagat tacgaagggg atagtttagc tgtcaactat ggcaaggtag gtagtattgg
    33541 taaattccat acaaaagaat tcgataatga agaacagtgt ctgaaagaag ccagtaaatt
    33601 gattgccgca aaaatgaaaa aaggatatca agaaactcca gactttaact tcatggatct
    33661 ctactatttt gatgatgaag agattgggtt acatcttaaa acgtcacacc caaacttcca
    33721 gtgccatttt actgatccac tttatatgtg ttgctgggat gaagaatctc cttttggtag
    33781 cgatgaaggt gctgatgctc taaacgaact tgaaaatagc ctccgttctg gactgtgctg
    33841 atttccctca aatgttaatt gaaactatgt ggggtatgaa atacatcgct atggacagta
    33901 ttcttgaaga ggatgtgcgt gcgcaattac tagccgatga aatgagctct atccagagca
    33961 atatgattac ctacgcgact gcattcggtc agatcaaagt catgggtaaa atctcccata
    34021 aacttaaaaa gatgggactc aatgcactag cgcgtcatca gcttaccgca aaaattcttc
    34081 aatggggtga cggtcaggac tcaccaatac ttcaaaaaat gattgatgac cttactgctt
    34141 ttccacacga aaattaaaaa ctgcatttgt cggtagcaac aactgccgac aaatcagatt
    34201 attgacaaag tgcactttgt ttatgccgga tgcggcgtaa acgcctgatc cggcataaaa
    34261 atattgctaa ttcaatatat tgcagaaaca ttgtaggcct gataagcgga gcgcatcagg
    34321 cagttttgcg tttgtcatca gtctccaatg ctattaatcc ttaaatccct gccccctgac
    34381 taaaatgctc ttccccaaac actccggtgg agaggtagcg atcgccacga tcgcagatga
    34441 tcgctaccac caccgcacca gggttagctt ttgccacccg cagtgctcca gcaaccgcgc
    34501 cgcctgagct gacgccacag aatattcctt cccgcaccgc cagttcgcgc atggtgtttt
    34561 ccgcatcgcg ctgatgaata tccagcacct catccaccag cgcagcgttg aaaatccccg
    34621 gcagatattc cgcaggccag cggcgaatgc ccggaatact gctgccttct tccggttgca
    34681 ggccaacaat ggtcaccggt ttggattgtt cgcgcataaa gcatgaaacg ccggtgatag
    34741 tgccggtcgt ccccatgctg gagacaaaat gagtgatgcg cccgccggtt tgctgccaga
    34801 tttccggccc ggtggtggtg taatgcgcat aagggttatc gggattattg aactgatcga
    34861 gcagctttcc ttcgccacga ttcgccatct ccagcgccag atcgcgcgca ccttccatgc
    34921 cctgctcttt ggtgacaaga atcagttccg caccataagc acgcatcgcc gcacggcgtt
    34981 cctggctcat gttgtcgggc atcagcaatt tcatgcgata gcctttcagc gcggcaatca
    35041 ttgccagcgc aatgccggtg ttaccactgg tggcttcgat taagacatca cccggtttaa
    35101 tttccccgcg cttttccgcc tcgacgatca tcgaaagtgc cgcacgatct ttcaccgaac
    35161 ctgccgggtt attgccttcc agttttaacc acacttcact gccgttatcc ggccccattc
    35221 gctgcaactt caccagaggc gtattgccta ttgtttgttc taatgtactc acaatctcta
    35281 tccatacgtg gtgttgcctg atgcgacgct tgcgcgtctt atcaggccta caggttacaa
    35341 accttgccat taaaaagccc ggatcgcggg aagcgcctcc gggcgtttaa cattcactca
    35401 acctatcagg cgctttgtgc gagagcaagt tcctcatcgc gggtttcaat acgctcgtcg
    35461 ccgttataca gccgcgcatg ttgcagacca acgaataaac gctcgccatg ctgcggggca
    35521 tcgtcgccat gcatcacgac cgtcagcggt tcgttgtacc accccagcgg ctgcaccact
    35581 aattgggtgt agtgaccttt cgggctggct tccagtacct gtaccggcag cggcgaatcg
    35641 agactggtac ggcggctgat atccacttcc cacgggcgca ggaagagatc caccggcccc
    35701 tgatacgcag gtgtgtagcc cagcggccag cgatgcgcgc caacatggaa ctgcccgccg
    35761 cgaatggttc cctgcaggcg gttcacttcg cccataaatt cgagtacaaa acgggtcgct
    35821 ggttcgcgcc atacctgatt cggcgcatca gcctgttcaa tattcccctg actcatcacc
    35881 actacacgat cggctacttc ggtcgcctct tcctggtcgt gggtcacaaa cacgctggtg
    35941 aatttcagct cttcatgcag ttgacgcagc cagcgacgca gctctttacg cacctgcgca
    36001 tccagcgcgc cgaacggttc atcaagcagc agaatttgcg gttctacagc cagcgcgcgc
    36061 gccagcgcca cacgctgttt ctggccgccg gaaagctgcg ccggataacg atccgccaga
    36121 tgcgcaagct ggaccatttc cagcaatttt gtcactttcg ctttgatggc tgcggcattc
    36181 gggcgctcgc gacgcggcag caccgtcagg ccaaaagtga tattgtcgaa taccgtcata
    36241 tggcggaaca gcgcgtaatg ctggaacacg aaaccgactt tacgatcgcg cgcgtgcagg
    36301 cgactcacgt cggtgccgtg gaagcgaata tgcccgctgg tttgatgctc cagcccggcg
    36361 ataatccgca gcagcgtggt tttcccggaa ccggacggcc ccagcaacgc gaccatctga
    36421 ccagaaggaa tatccagtga gatatcgttc agcacctggg tgcgaccaaa agacttcttg
    36481 atattggcaa tctcaatgct catgatgctc ctcctgctgc gcgcgttttt cctgattctc
    36541 caggcgccac tgcaacatac tctttaaaaa cagggtgata atcgccatca acgtcaacag
    36601 cgcggcggcg gtaaaggagc cgacggtgtt gtagtcctgc tccaacaatt caatctgtaa
    36661 cggcagcgaa agtgtttcgc cgcgaatcga gccggaaacc accgacactg caccaaactc
    36721 gccaatcgcg cgggcgttgg tcagcaccac gccgtaaagc agcgcccagc gaatattcgg
    36781 caatgtgacg cgacggaaca tctgccagcc ggacgcgcca agcaaaatcg ccgcttcgtc
    36841 ttcctggctg ccctggctta acatcaccgg caccagctcg cgcaccacaa acggacacgt
    36901 cacaaagatg gtgaccagca ccattccggg ccaggagaac ataatttgca gattatgttc
    36961 atccagccag ccgccgagcg ggccgttgga gccgtagaac agcagataca ccagaccggc
    37021 aaccaccggc gatacagcaa acggaatgtc cagtagcgtc agcagtaact ggcgtcccgg
    37081 gaagttaaag cgcgtcacca gccaggccag cagaatgccg aacaccaggt ttaccggtac
    37141 ggcaatcagc gcgatcatca ccgtcagcca gatggcgtgc agcatgtccg gatcggccag
    37201 attctgtaaa accggcatca gccccttgct gaatgcctgc acgaagatgt aaatcatcgg
    37261 caccagcagg atgaacgccg aaaccagcat cccgatgcca atcagaaacc atttgcccca
    37321 gttaatcggg cgcgcgtcat aacgcttcaa ttgggtaact tccgccatta atgacctacc
    37381 acacgccgac caaagcgact ttgcagagtg ttaattgaga acagcagcag cagagatgcc
    37441 gcgaggatca ccgaagcaat cgcgctcgct gccgggtaat caaactcctg taagcgcaca
    37501 aaaatcatca gcgacgtcac ttccgtcttc cacgcgatat ttccggcgat aaaaatcacc
    37561 gcgccaaatt caccaagact acgggtaaac gacagcgcca cgcccgccac cagcgccgga
    37621 gaaagctccg gcagcaccac tttgcagaaa ctctgccagc gcgttgcacc aagcgtttcc
    37681 gccgcttctt catattccgg gcctaactct tccagcaccg gctgcacggt acgcaccaca
    37741 aacggaatgc tggtaaaggc catcgccacc gcaataccaa gccaggtata ggtgactttg
    37801 atatcaaact tcgccagcca ttcaccgtaa aaaccgttta cggaaaagag cgaggccagc
    37861 gttaaaccgg cgactgccgt tggcagcgca aagggtaaat ccatcagcgc atcaagcagc
    37921 gtgcggcccg gaaagcgata acgtgttaaa atccacgcca tcagcagacc gaaaacgccg
    37981 ttaaaaatcg atgccacaaa cgccgacagc agcgtcactt tgtaggccgc gaccacctgc
    38041 gggttggtga tcacctccca gtactgcgcc cagctcatct cagaaagctg catcaccagc
    38101 gcagagagcg gcagcagcaa tatcaggcac acaaacagca gactggtgcc aaggcttaaa
    38161 gtaaagcccg gcagcacgcg tcttgaagaa actgcaaaca ttacttacgc cccgccgcta
    38221 acagcttgtc taactcgccg ccgctggtga agtgggtttt catcacttcc ggccatgagc
    38281 caaatttgtc ttccacgcgg aacagttcgg tctgcgggaa tttatctttc agtttgtcca
    38341 tgacttccgg gttattcaca cggtagtaat agtcggtgat gatggtctgc gcctgtgggc
    38401 tgtagagcca gttcaggtag gcttttgcgg ctttttccgt accgttggcc tgcacatttt
    38461 tatcgaccca cgccaccggg aattccgcca gaatattggt tttcgggatc accacttcaa
    38521 agccctgtgc ttcatactgt ttacggatgt tgttcacttc cgattcaaag ctaatcagca
    38581 catcgcccaa gccacgctcg gcaaaagtgg tggttgcgcc acgaccgccg gtatcgaaca
    38641 cttcaacgtt tttcaggaac tgggtcataa actgttcggt tttggcttta tcgccaccgt
    38701 cagctttatc cgctgcgccc catgccgcca gataggtata acgcgcgtta cccgacgttt
    38761 tcgggttcgg gaaaatcagc ttcacgtcgg agcgcaccag gtcgttccag tcgtggatat
    38821 tcttcgggtt gcccttacgc accaggaagc ccatggtgga gtagaacggc gagctattat
    38881 tcggcaggcg cgactgccag tcagccggga tcagcttgcc tttgtcgtgc aggatttgta
    38941 cgtcggtcac ctggttataa gtgacaacgt ctgcttttaa gccctgcaaa atcgccagcg
    39001 cctgttttga tgacccggca tgagattgtt ttatcgtcag tttgtcgccg ccgttatctt
    39061 ttgcccattg ctgttcaaac ggcggattca gggcggcaaa cagctcgcgg gagacgtcat
    39121 aagaactgtt cagcagttcc gttgcctgta catggcccgc cagcagcaga gaagcgacca
    39181 gcgcgagtga gttctttttc agtaagttaa cggccattgc gcacccttat aaatttaatg
    39241 actttctaat agccatcata tttataacgg acgtgaaagg agtaacggtt ttatataccg
    39301 tttggtgatt tggaagttaa aaagggaata agaacttggt tcaacaggcg taattgttgc
    39361 aggcagtttg gacacggaca gcgcggagaa accggagcgt acacatagta cgtgaggatt
    39421 tcgagcactg cccggggcca aaatgacaaa tgaaatagcc tggtgaactt agttcaagac
    39481 gaaatcctcc ccacacatgc agggaggaaa cagaggtgaa tcagataccg acgctaaccg
    39541 tctccggcag tgtgctgccg ccatcaatca cattctgtgt accggttaaa tagctggatt
    39601 catccgatgc gaggaaggcc gccagttcgc cgacttccag cggatcggcg aggcgacaca
    39661 gcgggattgc ttttgccatt tcagtcagca ccgattctgg atcttccggg ttcgactggc
    39721 gggcaatgct ttccgccatt ggcgtgcgga cgtatcccgg gcagatggcg ttaacgcgaa
    39781 taccggattg cgcgtactcc accgccagcg atttagtcag gccaacaatc gccgctttcg
    39841 ttaaggcata cgccgtttcg ccaggatcag ccaccatatc accagtgact gaagacatca
    39901 tcacaatgcg accatctttg cgcgcaatca tctccggcag caccgccttc gtgacgttcc
    39961 atacgccttt aatattgata tcaatatgga aatcgcgatc ttcatcgctc atatcgagga
    40021 aactgcccag acgacaaacg cctgcgttat tcaccaggat atcaatgcgc ccttcttttt
    40081 ccttcgcgcg tttgatagct gcggctaccg acgccgggtc acgcacatcg gcgacaaccg
    40141 ccgtacagcg atgaccacga ccacacagtt cgtccgctag cttttcgatc tcaggggaga
    40201 tatccagcaa gattaggttc gcgccatgac gtgcaaaagt tctggcaatt ccttcgccaa
    40261 ttccctgcaa tgcgcccgta atcagtgctg tcttgcccgt gagtttaccc attttaatgc
    40321 tctccttgtt aatacagagc gttacactcc attccctctg aaaatacagg ataaacaaaa
    40381 tattgctgcg gatcagctca ctaattcagg aaaatctcaa tccagtcgct gctttatgca
    40441 tcatttcagg cgttgtgtta gctcactgct gcgctgaatc attttcaatg acgccacatc
    40501 attgatgttg cacaagacca acaaacagta aatcggtaac agaattttgc gccgtgcggg
    40561 tagacatcga cgagctacgc cattcggttt caccggaaac ggtatcgagc gtgaaatgcg
    40621 ccagccgccg cagcggagag tcagccagag aagtaatggc aatcaccgtt gccccctgct
    40681 ttcgtgccgc ttctgcgcac aagacaatct cttttttact gccgctgtaa gagatagcga
    40741 tttgtacatc gccttttttc aacgcctggg aaacggtggc ttgtacgtgc gtgtcggctt
    40801 cgcaggccac gcgatagcca attttcatca gtttgaacga cagatcgcgc cccaccagcg
    40861 ccgatccgcc caggcccgtt atctggataa acggtgcttt actgatgacc tcaatgattt
    40921 tttgcagccg cgcgtagtcg aacaacgcac aggtttgctc cagcgccagc tctttttcgc
    40981 gattcagttt gcgggcaatc acttccagcg aatcatcact ggtaatcgaa ctgtgcaggt
    41041 gcagcgccgt ggcattcgtt ttctcccggc tggcactgta ttcgccaatt aacgccatac
    41101 gcaattcagt aaatccttgc gcgccgagct tctgggcaaa tttcacaatg ctcgactggc
    41161 tgataccgag ctgtttcgcc atctgccgtg aagagacgga tttcagctcg ctcactctgg
    41221 cccgcagaaa gtcagcaatt ttttgctcgt tcccggtaaa ttcactttcc gcattgcgta
    41281 ttttcgtcag gtacaacatc ttccactctc ctaggctggc agcaaaaatt ccgcttaagt
    41341 tcttatgacg ctcttcacac tctgcgagtg tagactcaat gattccttta agtctgtcaa
    41401 ttcgggatat atgattccat acatctgcaa ttatttgatc gtaaatagta aggtcaccac
    41461 cgatgcaact agaaaagatg attactgaag gctcgaacac cgcctcggct gaaattgacc
    41521 gcgtatcgac gctggaaatg tgccggatta tcaacgatga agataaaacc gtaccgcctg
    41581 ccgttgatcg cgtcctgccg gatatcgccg cggcgatcga tgttatccac gcccaggtca
    41641 gcggcggcgg tcgtctgatt tatctcggtg cgggaacatc cggtcgtctg gggattctgg
    41701 atgccagcga atgtccgccc acctacggcg tgaaaccggg tctggtggtt ggtttgattg
    41761 ctggaggcga atatgccatt cagcacgcgg tggaaggagc tgaagatagc cgggaaggtg
    41821 gtgttaacga tctgaaaaat attaatttaa cggcacagga tgtggtggtt ggcattgctg
    41881 ccagcggtcg cacgccgtat gtgattgccg gactggaata cgcacgccag ctcggctgcc
    41941 gcacagtggg aatttcctgt aatccgggga gcgccgtttc aaccaccgct gagtttgcca
    42001 ttacaccgat tgtaggtgcc gaagttgtta ccggttcttc gcggatgaaa gcaggtacag
    42061 cgcagaaact ggtgctcaat atgctttcca ccgggctgat gattaaatcc ggcaaagtgt
    42121 tcggcaacct gatggtcgat gtggtcgcca ccaacgaaaa actgcatgtg cgccaggtca
    42181 atatcgttaa aaacgccacc ggatgtaatg ccgaacaagc ggaagcggcg ttaattgcct
    42241 gcgagcgcaa ctgtaaaacg gccattgtga tggtgctgaa aaatctcgat gccgcagaag
    42301 ctaaaaaacg cctggatcaa cacggcggct ttattcgtca ggttttagac aaggaataac
    42361 ccatggccaa agagatcagc agtgaacttc tgaacaccat tcttacccgt gtcggcggac
    42421 cgggaaatat cgccagttgt ggtaactgta tgacgcgcct gcgtctgggt gtacatgaca
    42481 gttcactggt tgatcccaat atcaaaacgc tggaaggcgt gaagggcgtc attttgacca
    42541 gcgaccaggt gcaggtcgtt tttgggcctg gtaaagcgca tcgtgccgca aaagcgatga
    42601 gcgagctgct gggcgatgca ccggtacagg atgccgcaga aatcgccgcc cagaacaaac
    42661 gtcagttaaa agccaaacaa acctccggcg tgcaacaatt tctcgccaaa ttcgccacca
    42721 tcttcacgcc gctgatcccc ggttttattg ccgccggtct gctgctgggg atagcgacgt
    42781 taattgccac ggtgatgcac gttccggcag atgctcaggg cacgctaccc gacgcgctga
    42841 attttatgaa ggtgttcagc aaaggtttgt tcaccttcct agtgattctg gtgggctata
    42901 acgccgccca ggcatttggc ggcacgggcg tcaatggcgc aattattgcc gcactctttt
    42961 tgctcggtta taaccccgct gcaaccaccg gttactacgc cggttttcac gatttctttg
    43021 gtctgcccat cgatccgcgc ggcaatatta tcggcgtgct gattgccgcg tgggcctgcg
    43081 cccgcattga aggcatggtg cgccgcttta tgccggacga tctcgacatg ctgctgacct
    43141 cgttaatcac cctgctgatc accgctacgc ttgcgtacct gatcattatg ccgctgggtg
    43201 gctggttatt cgaaggtatg tcatggctgt ttatgcacct gaacagtaat ccgttcggtt
    43261 gtgcggtttt agccgggctg ttcctgatcg ccgtggtatt tggcgtgcat cagggcttta
    43321 ttcctgttta cctcgcgtta atggacagcc agggattcaa cagcttattt cccatccttt
    43381 caatggcagg cgcgggccag gtgggcgcgg cgctggcact ctactggcgg gcgcaaccgc
    43441 acagtgcgct acgcagtcag gtacgcgggg cgattattcc cggcctgctg ggcgttggtg
    43501 aaccgctgat ttacggtgtc accctgcccc gcatgaagcc gtttgttacc gcctgtttag
    43561 gcggcgcggc gggcggtttg tttatcggat tgatagcctg gtggggtctg ccgatgggct
    43621 taaacagcgc ctttgggccg tctggtctgg tggcgctgcc gctgatgact tccgcacaag
    43681 gcatccttcc ggcaatggcg gtttatgctg gcgggattct ggtggcatgg gtttgcgggt
    43741 ttattttcac cacgctcttt ggctgccgta acgtcaatct ggactgatat tatgaaacgg
    43801 acaatgctct atctttctct gctggctgtt agttgtagcg tcagcgccgc aaaataccct
    43861 gttctgacag aaggtagttg atcaagtata tgcggcgtta aagcagtaat aaaaaggcca
    43921 gtcagtaatg ttactgcctg gcctttaatc aatgaatcag aaacgcttac agcgccatca
    43981 acttgtccag cgacggtgcg aaataatagc cgccggttac cggtttggtg aaacgcaaca
    44041 tcgcatcacg tttgccatcg gtatcgccaa acatgctcag cagttgctgc tcaatgttat
    44101 gcagacgcgc gcagtaggcg cagaagtaca gaccgtgagt gccactggcg gtgccgtacg
    44161 gcaggctctg gcgaacaatc ttcagccctt tgccatcttc tttcagatca acgcgggtga
    44221 ggtgagaggt ttccggacgt tcgtcgccgt caatctcttc gttggcctct ttggtgcgcc
    44281 cgatcatcat ctcctgatcg tgaacgctca tccggttgag ctgcttcagg ttgtgctccc
    44341 aacgctgcac aaacacatag ctgccgccgg aatccacgcc gtctttgata accgccactt
    44401 cgcggcgcgt ctcttcaccc gccgggtttt ctgtaccgtc aacaaagccg ctcaggtcac
    44461 gctcttccac ccaacggaag ccgtggatct cttctttcac ttcaatgcag tcaccaaagg
    44521 cttccatcgc cgcctgagcg acagagaagt ttacgtcgtg acgcagagaa agaatgtgga
    44581 tcaacacatc gaactgggtc gtcggcgcaa ggcctttacc gtagcccgga aaatctttca
    44641 gctcttctgc cccaacgccg ccgctcagag cgcgccaggt gttgttacca aaggcaacca
    44701 ccgcaccaag atgcgcgtcc gggaatttcg cttcaaaagt tgccagtttg tcggcaaatg
    44761 ttttactggc cgcacgcagg gcgtcaactt cccctttcac gttggcttcg atccaaatcg
    44821 ccgcgcggca atgttctggc aaaatgccac tctgaacctg agacattgtt cctcctgaaa
    44881 ataagaatgc cacgcaatcc gtggcattgt tgagcgctat tttacgtgtt ttttcgcgac
    44941 ggcggtttgt tcagacgcaa attaacgccg ccagataatt ttactgactt tccagttttt
    45001 cagggtatcg tccgaaggca ttaacccttc aggaccacgc cattccccgc tgaactggta
    45061 gctaatatgt tgactgcctt cggctttaca ttcgacggca cgattatcat caccatcagc
    45121 tttttggcaa ttgccaaatg ctttgctgta aaggtcgcta aacggtgtac cgattttaac
    45181 accggtgtca gcaggaatat cgctatccag cacgtcaatg cgactgatcg tgccttgatc
    45241 gccgttaatc accatcgcca cgttgtcgcc tttcatcact tcaaaaaagc gcaccacgtt
    45301 gccgttcgtg gttttcattc cgctgcgcag gcgataatcg ccatcaagcg catcagcaat
    45361 ggcttgttct tgcagtggtg tggacgccgt taattcaccc acgccctgct cgctcacttt
    45421 ggtggacgat ccaaaccagt tccacgggtt agcggcagac cagttaactg acgacatcgt
    45481 ggaacagccg gtcagcatca acggcatagc gcataacatt aaacgcagcg atttcatgtc
    45541 acttcctttg gttattcaat aacgttgctt ggagtgcaaa ttcaccaaaa agtgccgtta
    45601 ttctttttct tcattaaaac aagcgcgtaa acgtcgattg gtcagcagcc agattaacgc
    45661 cacaatatcc gccactacca gcgccagacc gataccgcta acggattcac cgttcagcca
    45721 cagccacggt tgccagcaaa gtaaaaccac ctgcgccaac agcaacagaa aatagagcac
    45781 atgccaggtg cgaggaaacg aagcccgccg accgctcaac aaaaacgcca gcaccgccgg
    45841 aataccggga atcagcccca gccagaaatt atcgtgatcg ggataaaaca gatttagcag
    45901 cgcagtaccc tgctcacgcg acgcaccggc aatgacaaac agcacccagg ttcgcgcctg
    45961 aagcaatagc acaagccaga agagcaaggg taaacgcagg cgtccgtgcg catcataatg
    46021 gacaggatgg aactcagtac tcttcatctt caatcaaacg cttacccaga ctcagcacgt
    46081 cggcgtgttc atatcccagg cgttcataca ttccgagcac catgtcgtta tcttccggca
    46141 cgttgatctg aattttcggg cagccgcgag caatcagctt tttctccagc cggttaagca
    46201 acgcattggc aattccacgc ccacgaaact ctggatgcac gccaagataa taagcagacc
    46261 cgcgatgccc gtcataaccg cccatcaccg ttccgaccac ttcaccgttt acctcagcga
    46321 ccaaaaacaa actgacgtca tggttcatct tacgctcgat gtccatttcc ggatcgttcc
    46381 acggacgcag caagtcgcaa cgctcccaaa gggtgatgac ctcttcgaaa tcttcctggc
    46441 gaaatacgcg tatctccatg gtattcgtta cctttttgcg ggttaaaagg ctgattatgg
    46501 cgtgaacggt cgaattagcc aatatctgac gaaaatcggt tgaaaaagtg gcataatggg
    46561 gagttgtcaa ctattgaaat gaaaagtaaa acaattctca acagcaaacc gtcgtaacgg
    46621 attacgcgat acgatataac atctgaaact ttattattac aactcaggcc gtatgagcac
    46681 ttttaaacca ctaaaaacac tcacttcgcg ccgccaggtg ctgaaagccg gtttagctgc
    46741 cctgacgttg tcaggaatgt cgcaagccat tgccaaagac gaacctttaa aaaccagcaa
    46801 cggacacagc aagccgaaag ccaaaaaatc tggcggcaaa cgtgtcgttg ttctcgatcc
    46861 aggccacggc ggaattgata ccggagcgat cggacgcaac ggttcgaaag aaaaacatgt
    46921 ggtgctggcg attgctaaaa acgtccgttc cattttgcgt aatcatggga ttgatgcgcg
    46981 tttaacgcgt tctggcgata cgtttatccc actttacgat cgcgttgaaa tcgcccataa
    47041 acatggcgca gatctgttta tgtcaattca tgccgatggc tttaccaacc cgaaagctgc
    47101 cggtgcttcg gtatttgccc tctctaaccg tggggcaagt agcgcaatgg cgaaatacct
    47161 gtctgaacgc gaaaaccgcg ccgatgaagt tgccggtaaa aaggcgactg acaaggatca
    47221 cctattgcag caagtactgt ttgatctggt gcaaaccgat actattaaaa acagcctgac
    47281 gcttggctcg catattctga aaaagattaa gccggtgcat aaactgcaca gccgcaacac
    47341 cgaacaagcc gcgtttgtgg tgttgaaatc gccgtcggtt ccttcggtgc tggtggaaac
    47401 ctcgtttatc accaacccgg aagaagagcg gctgttaggc acggcggcgt ttcgtcagaa
    47461 aatcgccaca gcgattgctg aaggcgtgat cagttatttc cactggttcg acaaccagaa
    47521 agcacattcg aaaaagcgat aagttatgaa acccgacgca caccaggtta aacagtttct
    47581 gctcaacctt caggatacga tttgtcagca gctgaccgcc gtcgatggcg cagaatttgt
    47641 cgaagatagt tggcagcgcg aagctggcgg cggcgggcgc agtcgggtgt tgcgtaatgg
    47701 tggtgttttc gaacaggcgg gcgtcaactt ttctcacgtc cacggtgagg cgatgcctgc
    47761 ttccgccacc gctcatcgcc cggaacttgc cgggcgcagt ttcgaggcga tgggcgtttc
    47821 actggtagtg catccgcata acccgtatgt tcccaccagc cacgcgaatg tgcgtttttt
    47881 tattgccgaa aaaccgggtg cagagcccgt ttggtggttt ggcggcggct tcgatttaac
    47941 ccctttctat ggttttgaag aagacgccat tcactggcac cgcaccgccc gtgacctgtg
    48001 ccagccattt ggtgaagacg tttatccccg ttacaaaaag tggtgcgacg attacttcta
    48061 cctcaaacat cgcaacgaac agcgcggcat tggcgggctg ttctttgatg atctgaacac
    48121 gccagatttc gaccactgtt ttgcctttat gcaggcggta ggcaaaggct acaccgacgc
    48181 ttatttacca attgtagagc gacgtaaagc gatggcctac ggcgagcgcg agcgcaattt
    48241 tcagctctac cgtcgcggtc gttatgtcga gttcaatctg gtctgggatc gcggcacgct
    48301 gtttggcctg caaactggcg ggcgcaccga gtcgatcctg atgtcaatgc cgccactggt
    48361 acgctgggag tatgattatc agccaaaaga tggcagccca gaagcggcgt taagtgagtt
    48421 tattaaggtc agggattggg tgtaactccc tcacccccat tcccgcatcc gctgatgcaa
    48481 cgtcaacgac ggcttctcgg caaacagctg ctggtaatcc gtggcaaatt gccccagatg
    48541 ccagaatccc cactgcatgg cggcgtcttt taccgttgtg ctttgcgacc acgggcttat
    48601 cagttcgcgg cgtacgacgt tcaggcgaat gcgtttcagc cacgcgtttg ggccaatgcc
    48661 taaaatagcg tgaaacgcgt tttgtagcgt gcggcgactg acatgcagtt gattacacaa
    48721 atccagcacc gtcaccggtt cggacatgtt ttccagcaca tattcacggg cgcgggaaag
    48781 caaccgacgg taactctgat gactgatgct ttccgccgtc accattggtt gcgcttcttc
    48841 cagcatcgcc cccatcgcca ttagcaaatt atcccccagc acttttcgca ctgcaggctg
    48901 atggagattt tccgggttct cgcaaaacgt cgccagcgcc tgttggacaa agccccacag
    48961 cgcggcttta tgctgctctt tcacttccag cgccgactgg ctacgcagca tatgtaatac
    49021 ccgatccgga ttgtgcaaaa agttagcctg acaggtgatg acatcttcag acagcactac
    49081 gcccaggatc gtataatcgt ccggcgtact taattcaaac tcggttccac cggggcgcgt
    49141 cgctatttct gcgcttccca gacattgcga accgataaat ccctgctcac cgcgcgtcgc
    49201 cggaatgcca aaccagaacg agttcggcca gaccaggcac gactgacgca gcgccagacc
    49261 ggtgtattca cgaaaaacct gaatatcatc gagtagaatt tccgtaaatt caccatgaaa
    49321 cttgcccgga tgcagctgat cgtaaatctg ctgctaggcg gtaatcgtca aagcatgttc
    49381 atagacatcc gttgtctgtc gttgatgaac attatccact tcgaccttcg gcgtgagctt
    49441 caggttttcg ggtaagggtt catgataaag atggtgcaaa ttggctgtac gggtcttttt
    49501 catgatgtta atgccgggtg ttgtaggaca cccggcacct ccgacaggtt aatggggctt
    49561 gagacgataa cgactactgc gtttacgtaa cgtcccggca gaaaagagct gttcgagcgc
    49621 atttctggct ttttccagcg gccagccaaa gtgggcggcc acttctcctg ccgtcattcc
    49681 ctgacatact gatgttaaca gcgccaacaa ctcgtcagca gattccgcga cgataactgg
    49741 cgcgtcgggt tcctttacgg gttgcttcgg ctggcggtta aagccagtaa ccagccactg
    49801 ggtgtcatcg tctggccgac cgatctcctt acggctgatg acacgcccgg tacgcatcgc
    49861 ggcagcacaa cctgcgtcca gcgccgcacg acacgccgcc agatcgcctt ccaccaccag
    49921 cgttaagcga ccagggtcaa gcacttcgtg actgagcaga cgaacgttag ctgctttgag
    49981 catggcatcc gctgcatcta tcgcggcgac catgccgtcc acttccagca atcccagtgc
    50041 attgatcatc ggcaacctcc gttacgcacg ctggattgga ttacgcgcga tttccagcac
    50101 tgcatcggta aaggcgttac aggctgcttt acacgcggcc tggctaccgg ttaaaaatgc
    50161 tgccgagtag ttggtttcag acggtggcgg gacgtaggtc accagctgta cgtcggcaga
    50221 tttcaacgcg gcatcaatgc cgtaggtcgc ttccagcggc ggtgccacca gatacgccat
    50281 cggatcgcca agcgtgatcc cggcggttga tgaaagataa gaaccggtac gcgaaaccac
    50341 atgcgccagg aaggcggtgt tttctgcgtc attggcccac tggaaagctg cgccattttc
    50401 aatatgtgca accatcgcat ccagaccggc acgcacttcc gccgggtttg gcccaccaag
    50461 cataatcagc acttcaccgg cagtcggtga cgggccgtgt gccgctcccg catacagcga
    50521 gcgaccatat accacttcga ccatcgcttg tttggtcgct tcgtcagccg caatgtaggt
    50581 tacgtcgtca gaatcggcag aaatcagccc gaggctacga atatgcggcg gcaattttag
    50641 ttcacgcgca aattcagcgt taacggaggc aatcacccgc atggcggtta ccgacggtcg
    50701 aatcaaatct aaagctggca tgatgcctcc ttatcgggtc atgttgatgc cggatgcttt
    50761 ctgctccagc atacgtttcg ccaaatccac aatgacggca cagcagcacg aaagagaagc
    50821 aaaagtcagc tacctggtac actaggcagc gggtcagcaa aggggggcaa atgccccttt
    50881 tttcatgtca gataatcaga tcattacctc aataaactaa taatcacatc gcggttacaa
    50941 ccagtttgat agtgatagct caaacccaga cgtggcgctg gctgcaagca aaataagccg
    51001 caaaaatgcg accccatgtt tactagccgc aaaaatgcgg tcctatttgc cagaaccttt
    51061 tattattcaa ttggttagtg caagaaaaag cgtgattact gttcaagaag tatccagaaa
    51121 ggtcatgatt ttgacgctga aaaaaaatag gtgggcattt ttgcccactc actagccgca
    51181 aatttgccca ctcactagcc gcatttttgc ggcccaaagt aataaataca gagaaataat
    51241 aaatacagat cagcgccaag atttttcgtc ttttcggcgg aatcatgaga ctagtgctta
    51301 cgtcctgata acctccagag cagaccttaa cttctgtgca gcaggactac gctcattgcc
    51361 gctcggctag gtcaaatcct cacgataaag ctgttcggat tgtgaccata atgttaaagg
    51421 actctttaaa tcattccttg acgaattaac tagctggctt aagcggaata gcatgcagga
    51481 taaaaacaaa accagcttag cagtggggag agcttcgctt cgctcgtctc ctggggccaa
    51541 tgctcaatga aacgacgtaa ggcacggagc gctcaccata ggtagacaca taaacatctg
    51601 agagcgcacc ttctatcgcg ctacgcgctc aataaaaacc aacgacaccg ccacactgca
    51661 agaccaatgg tttttatcgc gtatacagac cagacaggaa acagcagcaa agcagcgtat
    51721 agccaggtgc agacagcaca ctcacacgac gcacagcagg cttaagtgat cactcaggaa
    51781 actttatccc cgccgccgat caccgcgaac tatcatgacc gcgccaaatt cgaaaggaat
    51841 ggcccataga acgcgatgaa cacacataag cgctacactt acttaacgtc gccacagagc
    51901 gattgagagc gcttaaaatc gatgcgattt cattaaattt ttctcactcg cctgaatggt
    51961 ggcgatcagg actgatcgca acattgcccc gcctcgatat tgagcgcata atagatacat
    52021 acattaagcg gaaaagagat aagtttattg tgaaaatcag ggggttacat atcatgcgca
    52081 tatcagaaca acaacggcag atcctcgact atctggcaaa aaacgaatca atcaccgcac
    52141 gacaagccgc agatctcgtt tatggtggca atgttacccg cgtccaggtt gacgccgccc
    52201 gccgctcact catgacgatg gttagtaatg ggctaatcag gaaacaaggc cgcgctttcg
    52261 tagctgaaag acatatcaat cacgcttata gccaggacgt tgaagaattg cgccgccaga
    52321 tcctttgggt attcagcaaa cagggtcagg agtatttcct ttctgaacac ccaaatttcg
    52381 ccgccaaaga gttaacagta agcggattgt cgtgcgcact ggattatctt gtcggtgaag
    52441 gttgcgttac ttacaagcag ctatggcacg ccctgaatac gcttgtagaa gatggtttac
    52501 tcattgccac gcgtgagaac atcgaatact ggataatgcc gcgttcaggt cgccgcaatc
    52561 atgcacggta tatttcaggc aacagataca gaacgccgga ctttaatccg acacaggaag
    52621 atcgggaccg tgccgaaatg atgtgcaggg aacaggagaa aagaattatg gataagttat
    52681 tttcgatgca gtgaacacaa gcgggcgcaa tgcccgtttt tattgcctta ttccacgcct
    52741 gacgaatcaa tttgatcaca cctgagaaaa tgcatagcca gaagttaagg ccgttatatt
    52801 gcgattggga gcgttttaaa cggtatcaat ttaattaatt ctgatgtggt gatttgcagg
    52861 caaaaaaaac ccgccggaga agcgggaaat agagacatat cgttgtagtt attaacagat
    52921 cactttgagg tcgagggcgg ctaaccctcg ctaatattat cccgctatta ccttgcgggt
    52981 gcaaataatc ttttattgct ttgatgattt aataaatagc actttttgct aacaaaaacc
    53041 gctgtcaata tatggccaaa aatgtcaaca gaaaaaataa ggtttataat attaagtatc
    53101 acatgggtaa tgggttcagg gaatcggaat ccaccgcctc acggaaattc aaaatttgct
    53161 tcttctttcc tccgtgggcg gcttcttttt cgtatcgagg tatttatgaa agcaataaca
    53221 ttagaacagt tcgcagcaat ggcgaatgta tcgaccagaa cgctgaggcg ctggctaaaa
    53281 aaaccatcaa ccggagtccc tttgccattt accccacgcg gtactcgcca attattctgg
    53341 gagcacgaat gcaaagaatg gttgtacaga acagaacgat aataatcacg catcacattt
    53401 tgaggtaaac atcatgacca atatcgaaag attagaacgc tgtgccgata ttatgcgacg
    53461 cagatggatt tatgaccctg aagcgggttt attgtttagc cgtgaaacaa aagacgttat
    53521 caaaggcagc atgaccaata aagggcattt ggttgtaacg gtacatgaag gtaaattcaa
    53581 agtgacactc acctatcaga aagcctgcta tgtatacaca tacggcgctt atgacgagac
    53641 attatatgaa gtgcaccacg ttaatttgaa caaacaggat aaccgcatca agaatattcg
    53701 cctgatgccg aaagaaaagc accgccggat acataaaaac gtccgcaaac ttattcggct
    53761 gggccacaca caatggcttc aaccgcaatt aattgaacaa tcactataaa cacaggagta
    53821 aataccatgc attcattaga cagttatttt cagcgcacga cagcccctaa atcagccgcc
    53881 caggaacgcc gtgaggaatt ccatgagaaa gtgatgcgtt ctgctgatta tattgcggac
    53941 aagttcgtgg aaacggttcg tccacttgtt gatgaagtag ctgataaact tcagtctgaa
    54001 atgccggaag atatggaagg cactgcaaag cggcgactca tttgcgaatt aagtcgtcgt
    54061 tttggcgtgt caataagcgc ttttaaataa tcgaaaaaag gggggaaaga taatggacgc
    54121 atttgaggta ctggcaaata aaggcgctga actggtagca cagcgtgata aaaccgccaa
    54181 cgaaggggaa cgcgcactgc taaataaaca gattaaagcg atccgtatgg cccagtttaa
    54241 attaatcagt aatgaagtta tcgaaacaat taaaccgcag atcccacaaa tcgttgctga
    54301 cgctattaaa gccgccggac tggagaaacg cattgccggg ctaaaaacga acgcagagaa
    54361 aatggagatg tacaaacaga gcggtgctcc gaatcccgat gagtacttct caccggatgt
    54421 tgagtttatg gcgcaggttg aggaacgtct gggtagctgt ctcaccgagg aacagcgtcg
    54481 ctattttgat ggagtggata gttcggcagg tattgacctg aactcgtatt tcggtcgtga
    54541 aattgagcat tttgatgccg ccccgtctat gccggagcca gaaccagaaa tggccaccga
    54601 tcctgaagct gagcaacgga aacgcgtatt caatgcgata gggattcggt actgatcgga
    54661 taaaagaaag ccgggaatta ccccggctga cttaacaatg tgaccaatga acaaatcgga
    54721 caagacaaac aggataagca gtctcgaaac aatgttcggg aactatatta gcacaagtcc
    54781 ggagaaaatc atcatgttgg cactacgcat catgcagggc atcgctaaga ccctcgcaga
    54841 acatgtctta gacctgaaac attcgccact gagcaaacag gccatgaagc gccaaacgct
    54901 tcgtctgtgg gctgaatatt cgcttggaac tatcaataaa atcatcgaca tgaaatcggg
    54961 gccgagcaac cagagcgcgg aggaaatgga gttcatcagg cgtttaattc tcattcgcag
    55021 agacattcat agccagttac acagcgtggg aatcgatatt aatgatggga cgggcgatta
    55081 attttcggac aagtactatg cgcgcgcgag gagctgaaca tgaagcaaga tattgcagac
    55141 aggctggaga tactcgaagg ccagcgggca gaagcgaaac aactacgtaa gcaggccaga
    55201 agagcgcaca ggaacaacga agcagagtta ctaaccaaat acatcagttt caccaattac
    55261 tgcatctacg agtgttacaa ggaagatgca gaggactggc tggattcatt gccagaacag
    55321 tactgagcag aataacacca cgtcatgttg ttctgaggtg agcatatcac ctctgttcag
    55381 gtggccaaat ttagtgtgcc acttcagcat caccgttgaa atcacaataa cgattttttt
    55441 gtaaatatgc tctcacatat gtaggaatag accaggttgg cattgaaaca catctac
//
LOCUS       DAZSIO010000004        55418 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZSIO010000004 DAZSIO010000000
VERSION     DAZSIO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.004"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="ACHX5T_000200"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="ACHX5T_000200"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR8465561.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="ACHX5T_000201"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="ACHX5T_000201"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR8465562.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="ACHX5T_000202"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="ACHX5T_000202"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR8465563.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="ACHX5T_000203"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="ACHX5T_000203"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR8465564.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="ACHX5T_000204"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="ACHX5T_000204"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR8465565.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="ACHX5T_000205"
     CDS             7826..9559
                     /locus_tag="ACHX5T_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR8465566.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="ACHX5T_000206"
     CDS             9708..10559
                     /locus_tag="ACHX5T_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8465567.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="ACHX5T_000207"
     CDS             complement(10546..10887)
                     /locus_tag="ACHX5T_000207"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR8465568.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="ACHX5T_000208"
     CDS             complement(10889..11767)
                     /locus_tag="ACHX5T_000208"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR8465569.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="ACHX5T_000209"
     CDS             complement(11733..14030)
                     /locus_tag="ACHX5T_000209"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR8465570.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="ACHX5T_000210"
     CDS             complement(14081..14401)
                     /locus_tag="ACHX5T_000210"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR8465571.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="ACHX5T_000211"
     CDS             complement(14416..15495)
                     /locus_tag="ACHX5T_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR8465572.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..18305
                     /gene="ptsP"
                     /locus_tag="ACHX5T_000212"
                     /pseudo
     CDS             15804..18305
                     /gene="ptsP"
                     /locus_tag="ACHX5T_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="ACHX5T_000213"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="ACHX5T_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="ACHX5T_000214"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="ACHX5T_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20973
                     /locus_tag="ACHX5T_000215"
                     /pseudo
     CDS             20366..20973
                     /locus_tag="ACHX5T_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="ACHX5T_000216"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="ACHX5T_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR8465573.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="ACHX5T_000217"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="ACHX5T_000217"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR8465574.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="ACHX5T_000218"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="ACHX5T_000218"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR8465575.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            25474..25608
                     /locus_tag="ACHX5T_000219"
     CDS             25474..25608
                     /locus_tag="ACHX5T_000219"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8465576.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="ACHX5T_000220"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="ACHX5T_000220"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR8465577.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="ACHX5T_000221"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="ACHX5T_000221"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR8465578.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="ACHX5T_000222"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="ACHX5T_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR8465579.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="ACHX5T_000223"
     CDS             30120..30728
                     /locus_tag="ACHX5T_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR8465580.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="ACHX5T_000224"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="ACHX5T_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR8465581.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="ACHX5T_000225"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="ACHX5T_000225"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR8465582.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="ACHX5T_000226"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="ACHX5T_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR8465583.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="ACHX5T_000227"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="ACHX5T_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR8465584.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="ACHX5T_000228"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="ACHX5T_000228"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR8465585.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="ACHX5T_000229"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="ACHX5T_000229"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR8465586.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="ACHX5T_000230"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="ACHX5T_000230"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR8465587.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="ACHX5T_000231"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="ACHX5T_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR8465588.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="ACHX5T_000232"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="ACHX5T_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR8465589.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="ACHX5T_000233"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="ACHX5T_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="ACHX5T_000234"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="ACHX5T_000234"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR8465590.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="ACHX5T_000235"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="ACHX5T_000235"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR8465591.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="ACHX5T_000236"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="ACHX5T_000236"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR8465592.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="ACHX5T_000237"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="ACHX5T_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR8465593.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="ACHX5T_000238"
     CDS             complement(44843..45142)
                     /locus_tag="ACHX5T_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR8465594.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="ACHX5T_000239"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="ACHX5T_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="ACHX5T_000240"
     CDS             45895..46494
                     /locus_tag="ACHX5T_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR8465595.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="ACHX5T_000241"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="ACHX5T_000241"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR8465596.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="ACHX5T_000242"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="ACHX5T_000242"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR8465597.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="ACHX5T_000243"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="ACHX5T_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR8465598.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="ACHX5T_000244"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="ACHX5T_000244"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR8465599.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="ACHX5T_000245"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="ACHX5T_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR8465600.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="ACHX5T_000246"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="ACHX5T_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR8465601.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="ACHX5T_000247"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="ACHX5T_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR8465602.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="ACHX5T_000248"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="ACHX5T_000248"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR8465603.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="ACHX5T_000249"
     CDS             complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="ACHX5T_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR8465604.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13494 a        15017 c        14163 g        12744 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttat attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt tagtttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatcgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatatggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg gatcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctagg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgatgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcac gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaacacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtatg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggtgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttat tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    55081 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    55141 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    55201 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    55261 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    55321 ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    55381 tagcaaaccg gacgaacgtt agttcatccg gctgtggg
//
LOCUS       DAZSIO010000005        54516 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZSIO010000005 DAZSIO010000000
VERSION     DAZSIO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.005"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            133..765
                     /gene="comR"
                     /locus_tag="ACHX5T_000250"
     CDS             133..765
                     /gene="comR"
                     /locus_tag="ACHX5T_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR8465605.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(827..1366)
                     /locus_tag="ACHX5T_000251"
     CDS             complement(827..1366)
                     /locus_tag="ACHX5T_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR8465606.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1593..2897)
                     /gene="ndh"
                     /locus_tag="ACHX5T_000252"
     CDS             complement(1593..2897)
                     /gene="ndh"
                     /locus_tag="ACHX5T_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR8465607.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3305..3847)
                     /gene="ycfP"
                     /locus_tag="ACHX5T_000253"
     CDS             complement(3305..3847)
                     /gene="ycfP"
                     /locus_tag="ACHX5T_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR8465608.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3870..4895)
                     /gene="nagZ"
                     /locus_tag="ACHX5T_000254"
     CDS             complement(3870..4895)
                     /gene="nagZ"
                     /locus_tag="ACHX5T_000254"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR8465609.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(4906..5730)
                     /gene="thiK"
                     /locus_tag="ACHX5T_000255"
     CDS             complement(4906..5730)
                     /gene="thiK"
                     /locus_tag="ACHX5T_000255"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR8465610.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5711..6352)
                     /gene="lpoB"
                     /locus_tag="ACHX5T_000256"
     CDS             complement(5711..6352)
                     /gene="lpoB"
                     /locus_tag="ACHX5T_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR8465611.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6367..6744)
                     /locus_tag="ACHX5T_000257"
     CDS             complement(6367..6744)
                     /locus_tag="ACHX5T_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR8465612.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6747..7106)
                     /gene="hinT"
                     /locus_tag="ACHX5T_000258"
     CDS             complement(6747..7106)
                     /gene="hinT"
                     /locus_tag="ACHX5T_000258"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR8465613.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7439..9624
                     /gene="fhuE"
                     /locus_tag="ACHX5T_000259"
                     /pseudo
     CDS             7439..9624
                     /gene="fhuE"
                     /locus_tag="ACHX5T_000259"
                     /inference="COORDINATES: protein motif:HMM:NF007447.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(9684..11117)
                     /gene="ptsG"
                     /locus_tag="ACHX5T_000260"
     CDS             complement(9684..11117)
                     /gene="ptsG"
                     /locus_tag="ACHX5T_000260"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR8465614.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11412..12209)
                     /locus_tag="ACHX5T_000261"
     CDS             complement(11412..12209)
                     /locus_tag="ACHX5T_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR8465615.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12220..13224)
                     /gene="holB"
                     /locus_tag="ACHX5T_000262"
     CDS             complement(12220..13224)
                     /gene="holB"
                     /locus_tag="ACHX5T_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR8465616.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13221..13862)
                     /gene="tmk"
                     /locus_tag="ACHX5T_000263"
     CDS             complement(13221..13862)
                     /gene="tmk"
                     /locus_tag="ACHX5T_000263"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR8465617.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13852..14874)
                     /gene="yceG"
                     /locus_tag="ACHX5T_000264"
     CDS             complement(13852..14874)
                     /gene="yceG"
                     /locus_tag="ACHX5T_000264"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR8465618.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14877..15686)
                     /gene="pabC"
                     /locus_tag="ACHX5T_000265"
     CDS             complement(14877..15686)
                     /gene="pabC"
                     /locus_tag="ACHX5T_000265"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR8465619.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(15806..17047)
                     /gene="fabF"
                     /locus_tag="ACHX5T_000266"
     CDS             complement(15806..17047)
                     /gene="fabF"
                     /locus_tag="ACHX5T_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR8465620.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17134..17370)
                     /gene="acpP"
                     /locus_tag="ACHX5T_000267"
     CDS             complement(17134..17370)
                     /gene="acpP"
                     /locus_tag="ACHX5T_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR8465621.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17581..18315)
                     /gene="fabG"
                     /locus_tag="ACHX5T_000268"
     CDS             complement(17581..18315)
                     /gene="fabG"
                     /locus_tag="ACHX5T_000268"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR8465622.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18328..19257)
                     /gene="fabD"
                     /locus_tag="ACHX5T_000269"
     CDS             complement(18328..19257)
                     /gene="fabD"
                     /locus_tag="ACHX5T_000269"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR8465623.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19273..20226)
                     /gene="fabH"
                     /locus_tag="ACHX5T_000270"
     CDS             complement(19273..20226)
                     /gene="fabH"
                     /locus_tag="ACHX5T_000270"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR8465624.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20294..21364)
                     /gene="plsX"
                     /locus_tag="ACHX5T_000271"
     CDS             complement(20294..21364)
                     /gene="plsX"
                     /locus_tag="ACHX5T_000271"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR8465625.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21445..21618)
                     /gene="rpmF"
                     /locus_tag="ACHX5T_000272"
     CDS             complement(21445..21618)
                     /gene="rpmF"
                     /locus_tag="ACHX5T_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR8465626.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21670..22191)
                     /gene="yceD"
                     /locus_tag="ACHX5T_000273"
     CDS             complement(21670..22191)
                     /gene="yceD"
                     /locus_tag="ACHX5T_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR8465627.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            22390..22974
                     /gene="yceF"
                     /locus_tag="ACHX5T_000274"
     CDS             22390..22974
                     /gene="yceF"
                     /locus_tag="ACHX5T_000274"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR8465628.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23086..24045)
                     /gene="rluC"
                     /locus_tag="ACHX5T_000275"
     CDS             complement(23086..24045)
                     /gene="rluC"
                     /locus_tag="ACHX5T_000275"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR8465629.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24618..27809
                     /gene="rne"
                     /locus_tag="ACHX5T_000276"
     CDS             24618..27809
                     /gene="rne"
                     /locus_tag="ACHX5T_000276"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR8465630.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(28006..28948)
                     /gene="flgL"
                     /locus_tag="ACHX5T_000277"
                     /pseudo
     CDS             complement(28006..28948)
                     /gene="flgL"
                     /locus_tag="ACHX5T_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(28960..30602)
                     /gene="flgK"
                     /locus_tag="ACHX5T_000278"
                     /pseudo
     CDS             complement(28960..30602)
                     /gene="flgK"
                     /locus_tag="ACHX5T_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(30668..31609)
                     /gene="flgJ"
                     /locus_tag="ACHX5T_000279"
     CDS             complement(30668..31609)
                     /gene="flgJ"
                     /locus_tag="ACHX5T_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR8465631.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31609..32706)
                     /gene="flgI"
                     /locus_tag="ACHX5T_000280"
     CDS             complement(31609..32706)
                     /gene="flgI"
                     /locus_tag="ACHX5T_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR8465632.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32718..33416)
                     /gene="flgH"
                     /locus_tag="ACHX5T_000281"
     CDS             complement(32718..33416)
                     /gene="flgH"
                     /locus_tag="ACHX5T_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR8465633.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33469..34251)
                     /gene="flgG"
                     /locus_tag="ACHX5T_000282"
     CDS             complement(33469..34251)
                     /gene="flgG"
                     /locus_tag="ACHX5T_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFR8465634.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34389..34976)
                     /gene="flgF"
                     /locus_tag="ACHX5T_000283"
                     /pseudo
     CDS             complement(34389..34976)
                     /gene="flgF"
                     /locus_tag="ACHX5T_000283"
                     /inference="COORDINATES: protein motif:HMM:NF009280.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(35078..35140)
                     /locus_tag="ACHX5T_000284"
                     /pseudo
     CDS             complement(35078..35140)
                     /locus_tag="ACHX5T_000284"
                     /inference="COORDINATES: protein motif:HMM:NF012674.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35160..36299)
                     /locus_tag="ACHX5T_000285"
     CDS             complement(35160..36299)
                     /locus_tag="ACHX5T_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR8465635.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGANNIVATTQNGYKPGDLVSYQINDDGTV
                     VGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTN
                     GALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR"
     gene            complement(36324..37019)
                     /gene="flgD"
                     /locus_tag="ACHX5T_000286"
     CDS             complement(36324..37019)
                     /gene="flgD"
                     /locus_tag="ACHX5T_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR8465636.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37031..37434)
                     /gene="flgC"
                     /locus_tag="ACHX5T_000287"
                     /pseudo
     CDS             complement(37031..37434)
                     /gene="flgC"
                     /locus_tag="ACHX5T_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(37438..37854)
                     /gene="flgB"
                     /locus_tag="ACHX5T_000288"
     CDS             complement(37438..37854)
                     /gene="flgB"
                     /locus_tag="ACHX5T_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR8465637.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38009..38668
                     /gene="flgA"
                     /locus_tag="ACHX5T_000289"
     CDS             38009..38668
                     /gene="flgA"
                     /locus_tag="ACHX5T_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR8465638.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            38744..39037
                     /gene="flgM"
                     /locus_tag="ACHX5T_000290"
     CDS             38744..39037
                     /gene="flgM"
                     /locus_tag="ACHX5T_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR8465639.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39042..39458
                     /gene="flgN"
                     /locus_tag="ACHX5T_000291"
     CDS             39042..39458
                     /gene="flgN"
                     /locus_tag="ACHX5T_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR8465640.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(39498..41033)
                     /gene="murJ"
                     /locus_tag="ACHX5T_000292"
     CDS             complement(39498..41033)
                     /gene="murJ"
                     /locus_tag="ACHX5T_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR8465641.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41200..42066)
                     /locus_tag="ACHX5T_000293"
                     /pseudo
     CDS             complement(41200..42066)
                     /locus_tag="ACHX5T_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(42068..42715)
                     /locus_tag="ACHX5T_000294"
     CDS             complement(42068..42715)
                     /locus_tag="ACHX5T_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HFR8465642.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42726..43310)
                     /gene="rimJ"
                     /locus_tag="ACHX5T_000295"
     CDS             complement(42726..43310)
                     /gene="rimJ"
                     /locus_tag="ACHX5T_000295"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR8465643.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43546..44754
                     /gene="mdtH"
                     /locus_tag="ACHX5T_000296"
     CDS             43546..44754
                     /gene="mdtH"
                     /locus_tag="ACHX5T_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR8465644.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44818..45465
                     /gene="grxB"
                     /locus_tag="ACHX5T_000297"
     CDS             44818..45465
                     /gene="grxB"
                     /locus_tag="ACHX5T_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFR8465645.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            45599..46159
                     /locus_tag="ACHX5T_000298"
     CDS             45599..46159
                     /locus_tag="ACHX5T_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR8465646.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46265..47311
                     /gene="pyrC"
                     /locus_tag="ACHX5T_000299"
     CDS             46265..47311
                     /gene="pyrC"
                     /locus_tag="ACHX5T_000299"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR8465647.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47385..47630
                     /gene="dinI"
                     /locus_tag="ACHX5T_000300"
     CDS             47385..47630
                     /gene="dinI"
                     /locus_tag="ACHX5T_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR8465648.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            47920..48174
                     /gene="bssS"
                     /locus_tag="ACHX5T_000301"
     CDS             47920..48174
                     /gene="bssS"
                     /locus_tag="ACHX5T_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR8465649.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            48289..49407
                     /gene="solA"
                     /locus_tag="ACHX5T_000302"
     CDS             48289..49407
                     /gene="solA"
                     /locus_tag="ACHX5T_000302"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR8465650.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            49455..49568
                     /locus_tag="ACHX5T_000303"
     CDS             49455..49568
                     /locus_tag="ACHX5T_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFR8465651.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            49829..50395
                     /locus_tag="ACHX5T_000304"
     CDS             49829..50395
                     /locus_tag="ACHX5T_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR8465652.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            50399..50974
                     /locus_tag="ACHX5T_000305"
     CDS             50399..50974
                     /locus_tag="ACHX5T_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR8465653.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(51016..52068)
                     /locus_tag="ACHX5T_000306"
     CDS             complement(51016..52068)
                     /locus_tag="ACHX5T_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR8465654.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            52293..53213
                     /gene="lpxL"
                     /locus_tag="ACHX5T_000307"
     CDS             52293..53213
                     /gene="lpxL"
                     /locus_tag="ACHX5T_000307"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR8465655.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            53385..53570
                     /locus_tag="ACHX5T_000308"
                     /pseudo
     CDS             53385..53570
                     /locus_tag="ACHX5T_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            53606..>54516
                     /locus_tag="ACHX5T_000309"
     CDS             53606..>54516
                     /locus_tag="ACHX5T_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR8465656.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGII"
BASE COUNT        13330 a        14499 c        13438 g        13249 t
ORIGIN      
        1 attaattaat ttaaaaatct ttaaatttca gtcaaataga aagcaattat ttttatggga
       61 tctggactgg tgaacggagg tgattatttt tataataacc tttcattcac agtcaccagg
      121 tacaacggca tcatggcaac tgactcaaca caatgtgtaa aaaaaagccg tggccgccca
      181 aaagtgttcg acagggatgc cgcgcttgat aaggccatga aattgttctg gcaacacggt
      241 tatgaagcga cttctcttgc ggacctcgtc gaagcaaccg gagccaaagc gcccacgcta
      301 tacgcggaat ttaccaacaa agaggggtta tttcgtgccg ttctcgaccg ctatatcgat
      361 cgttttgccg ctaagcatga agcacagctg ttttgtgaag agaaaagcgt ggagtctgcg
      421 ctggctgact attttgctgc catcgccaac tgctttacca gcaaagacac cccggcaggc
      481 tgcttcatga tcaacaactg caccaccctc tccccagatt caggagatat agccaatacg
      541 ttgaaatcac gccatgcgat gcaagagcgc actttgcagc agtttttatg tcaacgacaa
      601 gcgcgcgggg aaatcccgac ccactgtgac gtgacacatc tggcagaatt ccttaattgt
      661 attattcagg ggatgtcgat cagcgcacgc gaaggtgcat cgctggaaaa actgatgcag
      721 attgccggaa cgactttgcg tttatggccc gaactggtga aataagtcgc aaaaaaggcc
      781 cctcagcctg agcgaatgag gggccaaatt gcagagtaca gccttgttat actttgttat
      841 tcaaaatcag ttgcccgtta ctatctagcg ggatctgcgt acccggatcg cggtccatgc
      901 ggattttgcc ctgctgatcg ccaatcttat aggtcacatc ataaccgagc attttttctg
      961 acttgtcata caccgtttta caacgctgtt gcgtagtcgt gtaagtatcg ctttcctgga
     1021 gagagccctg gatctggtta ccggcatatc caccacccag cgcccccaca acagtggcga
     1081 catctttacc gcgaccacca ccaaactgat gccctatcac gccaccagca acagcgccga
     1141 gcaccgaccc ggtaatgcga ttttcatcct gcaccggtcg acgatgggtc actgtgacgt
     1201 tgcgacactc ctgacgcggt gttttaaccg tttccttgat tggggttgca gaaacaacct
     1261 gagcgtattg cgggccccgt tcaaacacgt tcagactggc cactgccgct acgcccaacg
     1321 cagctgcgac accaatcccg atacccgcca acattgattt attcacggga acacctcctt
     1381 ctttgcagtt atgccaatca tgcaagcgaa ggagaaaagc agctatcaga cagaagatga
     1441 aaagcgcggg aatgactgga aatacttaag gattcggagg agtctgaagg aatgcctgat
     1501 gcgacgctta acgcgtctta tcaggcctac cgctcgtacc atttgtccag ggcggataag
     1561 gcgtttatac cacatccgcc agtgtacgtc gattaatgca acttcaaacg cggacggata
     1621 acgcggttaa tactccccac cagcatcatt aatccggttt taaagtaacc atgcagcgca
     1681 atctgatgca ttcggtatag cgagatatat acaaagcgcg caattcgtcc ttcaatcatc
     1741 attgagccgc gcgtcaggtt acccatcagg ctaccaacgg tggagaagtt cgacagcgat
     1801 accagcgaac catgatcttt atactgataa tttttcagcg gcttaccgtt catctgcgcc
     1861 agaatgttgt tcattgcgca agtcgccatc tggtgtgcag cctgagcacg cggcggaaca
     1921 aagccccctt ccggacgcgg gcatgacgcg cagtcgccaa tagcgtaaat gtctggatcg
     1981 cgagtggttt gcagcgtcgg ttccaccacc agctggttga tacggttagt ttcaagacca
     2041 ccgatatctt tcaggaagtc tggcgctttg atcccggctg cccataccat cagatcagcc
     2101 tcaatatatt cgccatcttt agtgtgcagg ccgccttcat cagcactggt gaccatggtt
     2161 tgcgtcagca cgcgaacgcc aagtttcgtt agctcgttgt gggccgcagc agagatacgt
     2221 ggcggtaatg caggcaaaat acgttctccc gcttctacca gcgttacgtt cagggcttcg
     2281 ttggtcaggc ctttgtaacc gtagctgtgc agttgcttga tcgcgttgtg caattcagcg
     2341 gagagttcta cacccgtcgc gccgccgccg acaatcgcaa tgttcacttt gccatttgcg
     2401 cccaggttgg cagagtattt caggaacaga ttcagcatct cctggtggaa gcgacgcgcc
     2461 tggtgcgggt tatcgaggaa aatgcagttc tctttgacgc ctggcgtatt gaaatcgtta
     2521 gaggtgctac ccagcgccat gaccagggtg tcataggcga ttttacgttc cggaaccaac
     2581 agttcacctt tctcgtcgcg tagttctgca atagtgattg ttttcgcttc acgatcaata
     2641 tcaatgacgg aacccaactg gaactggaaa ccatgattgc gcgcatgggc cagatagctc
     2701 aacgcatcga cgccttcatc aagcgagcca gtcgccactt cgtgcagcag tggtttccac
     2761 aggtggctgt ggttacgatc gaccagcgta attttggctt ttttcttacg tcccagctta
     2821 tgccccagct gtgttgccat ttccagccca ccagcaccgc cgccgacaat cacaatcttt
     2881 ttcaatggcg tagtcaacgt gcccccctta aatttattaa ccaattgtta attaatggtt
     2941 attaacatag ctcttaatta acaacaggtt acagcaatga aaatgttctt cgctattgag
     3001 aataacatga atggtgcatt ggtcatacca aaattgatgt taatcaagtt ttgttgctta
     3061 aaagataggc aactttaccg ggggagatca aaaaatagag tttattgttt attatttgag
     3121 caaattcgga agggtaatat agggtgaagc gtatatcgag ggcgtttggt cgtcctgata
     3181 agatgcgcag catcgcatca ggcattgatg ctcgactgtc ggatgcggcg tggacgcctt
     3241 atccgactta cagaattcgg agcgcatcag gtaccggcaa ggttacctga tgcgacgagg
     3301 gcatttaccc gagggttttg aacgctttaa tgcgctgtaa atgcggggag atattcttga
     3361 atttgtgcgt ctgctcttcg tcccagacaa tctcgtaata atgatgcaac tcttcagatg
     3421 tccgctggct gttaagtgct tcatcattac gcgacaaaat aaccagacaa cgatcgcgat
     3481 tcttctcgcg gaagttggtc acacacttag tggcaatatc ggcatactct tccgggcgat
     3541 caatcttgcc ttccatgttc tcataaggga acaaattagg gttgaagatc acctggcgga
     3601 tatcgcaaag aaaaccaatc cgttccgccc agtatccgcc caggccaacg ccgcaaatta
     3661 gcggacgctc gtcaacgttc agttgcaaca ttttgtccac ttctttaagc agatgctgca
     3721 tatcatgttt cggatgccgc gtactgtagc ttatcaagcg tacatccggg tcaataaact
     3781 gcaattgtaa gactttctcg tggttacccg gactgttaga gtcaaaaccg tgtaaataga
     3841 taatcatcgc ttcctcacat aagccagggt tagtgaccta ctttctcttc ctgccagcgt
     3901 tcatgtaact gattcagacg ggtgctgatc gctttccagc gagccgagtc catcagttcc
     3961 tgtcgcgaaa atgaaccttt atgatacaaa cgtgtaacac gttctgcctt gatcggtgac
     4021 agattatcta acacgctgac ggccccttta cgattattgc agaccaggat catatcgcaa
     4081 cccgcatcca gtgatgcctg cccgcgttcg gcataactgc ccataatcgc ggcaccttcc
     4141 atcgataaat cgtcagagaa aatcacgccg tcaaacccca gttcctgacg caaaacggtt
     4201 ttcagccagt agggagaacc gctcgccgga cgcggatcaa catcactgta gatcacatgc
     4261 gcaggcataa tggcgtcgag tttattttcg cggattaagg aactgaagac cgacatatct
     4321 ttagcgcgaa tctccgcttg tggacgtgga tcgcacggtg tttctttgtg tgagtctgcc
     4381 gttactgcac cgtgtcctgg gaagtgtttc ccggtcgttt tcattccggc ttcatgcata
     4441 ccatcaataa accggctggc aattgccagg gctttttgtg gatcggcatg ataagaacgc
     4501 tcgccaatcg ccgcgctgat atgcccgaca tccagcactg gcgcaaagct gatatcaata
     4561 tccatagcga tcatttcgct ggccatcagc caacccgcct cttgcgccag tttgccgccc
     4621 tcttccattc ccaacagcgc agcaaaggat tgtgctgccg gtaagcgggt aaaaccttcg
     4681 cgaaaacgct gcacgcgtcc accttcctga tctaccgcca ccaccagatg attgcgcgat
     4741 gctgcgcgga tctggcgcac cagttcacgt aactgggcag gatcatgata gttacgcgta
     4801 aagagaatca gccctcccac cagcggatgc gccagtattt cacgctcttc cgcgtccagt
     4861 tcgtaacctt cgacatccaa cattactgga cccacactgc tctccttatt gttttattaa
     4921 tagctgccgc caggtgtcat cggccagcct gataaattgt tgatcgccgg tttgtcgcca
     4981 gcggtactca aaccaccctc ctttgagcat cagcagccag ggaaaccatc gcctgacctg
     5041 acgccataat tgcgccggat aaatcttcgc gcgagtggca tagtcattga ccaattgccg
     5101 gtgctgttca gtattttcca cccacaccgc cgccagttcc agcgcgatat caccatctcc
     5161 agcatactcc cagtcgatga gttttaaccc tgatgcgcta tgcactaaat ttccggcgtg
     5221 gacatccata tgcaatggac ttaagcgtaa aggtcgtggt tcccgcgctt tgcgcagacg
     5281 ttttaacatt cgcagccaac ccacagtccg ccgcgccgga tcgctttgct gccagtacag
     5341 ttccagtaag ggcaacagcg ttattcgcca gccaaaacgt ggttgttgat gtagataata
     5401 cagcaagcct gccagttcgt tggtatccgg caaatacgtt tttaccgcac cgggcagata
     5461 gtcgactacc atccagtcac ggagatataa atgcggcttc ggtgcaatgc ttgcgggtag
     5521 ttgtgataaa gcccgatact ggcgcaagaa cgcggactgc ggcgcatcag gatcgtgcgg
     5581 ctgacgcaca acaaaacgct ggccctgatg ttcaatgaga aaactaccgc cactaagccc
     5641 actattaaac gtcgtgacgg gatgaaactg cgggaaaaag cgcgacagca attcgtcgcg
     5701 cgtgatggga ttattgctgc gaaacggcac ctttacctga ccagataatt tcgcccgtct
     5761 gcaccagcat cagctgcatt tgtagggtcg gagcgttaac gttgccagag gcgcaggagt
     5821 acagcacgta atgagcgccg acattgcggg caatgcctat ggctttacta cgggtgccta
     5881 aactgtcctg cggcgacaaa cctaactgtt gcttagccat cgacagttgc tgggcggaaa
     5941 ccagggtaaa tttcccgtta ttagccagcg catttcgcag cgtttcggtc gcttctgcgg
     6001 cattcagcga accgttagta cggttgttaa cgctatcaac cagcaggacg ctacccgcag
     6061 tcaccccgtc agccccaagc atcttactga ccatcggctg cattgcgcca ttccagtcat
     6121 aatggcgaat atgcggcgca ggcggtgccg ttcgatcttc gtgctcaatt gggcctggct
     6181 gctgcgggat cgtcggcacc gagggcactg tggggacagg ctgttgtggc tcggctggtt
     6241 gttccggcgc tggtttcact tcttctaccg gtgcaggttc acgttgcccc acacacccgg
     6301 cgagaaacat cgccagcgcg gtaatcaagg cgtagcgact catttttgtc atcaagattc
     6361 acccctttac aaatatagat aaagtctgac tttgtgcgcc cccagaaaat tggcgctgcc
     6421 gtacagcgtt accgccgaat gtgcgggaat ggtaacgctg cgtggccttt ccagaggatg
     6481 catctccagc cctctggcgt cataccagta aaaacgataa tgaacggtaa cgggttcttg
     6541 cctttcgtta taaatcgttg aggatgctga aggttgaata tcagacgtcg aaaggacggg
     6601 cttttctgca ctgatgcccg cagccaataa agatgactcc atcaccagcg attgctcatc
     6661 attcaccgga atttccggat gtgaacgaca gcccaccagc agtaacaaca ccagagacac
     6721 cagcccaaag catccttttc tcatcgttac agacctttat gcgccagcat tggtcccagc
     6781 ggacggccac ccaacaagtg catatggatg tggtaaacct cttgtccgcc atggcggttg
     6841 gtattcatga tcagacgata gccatcttcg gcaatacctt cttgctcagc aatttttgcc
     6901 gctacggtga tcatgcgtcc cagcgcctgc tcatgctcag ctgacacgtc gttcacagtc
     6961 ggaatgagaa tattcggaat gatcagaata tgcgttggcg cttgcggcga aatatcgcga
     7021 aacgccgtta ccagatcatc ctggtagacg atatcggagg ggatctcacg acgaataatt
     7081 ttgctgaata tagtttcttc tgccacgaca ttttcctttt tcaaatagcc atgcgttgcg
     7141 ccatgctacg ccgcgccaca ctgcgagtat agagtatggt cgagttaaac ctgctctttc
     7201 aatcttaact cacgatttct taagcaaaaa atcgttttgc tgccgtatat atcgccatta
     7261 ttcccatttc tgcctgcgaa tgcgctgaaa agacattctc tgtttggttt acacgacaaa
     7321 tatgaatgcg tatatttctc atttgcattt gcagcaaaat tattcgcaca ctaaatataa
     7381 ccgctcatca ccctcctccg gcatgagcct gacgacaaca taaaccaaga gatttcaaat
     7441 gctttcaaca caatttaaca gggataatca atatcaagtc atcaccaaac cgtcactact
     7501 tgccggttgc atagcactgg cactattacc ttctgccgct tttgctgcac cagccactga
     7561 agaaacggtg attgttgagg gttcagccac agctccagat gatggcgaaa atgattacag
     7621 cgtaacgtct acctctgcgg gtaccaaaat gcagatgact caacatgata ttcctcagtc
     7681 ggtcactatt gttagccagc agcggatgga agatcagcag ttacaaacgc tgggcgaagt
     7741 gatggaaaac acgctgggga tcagcaaaag tcaggcggat tccgatcgtg ctctttatta
     7801 ttcccgcgga ttccagatcg ataactatat ggttgatggt atccccacct attttgaatc
     7861 gcgctggaat ctgggcgacg cactttctga tatggcactg tttgaacgcg tagaagtagt
     7921 gcgtggcgcg acaggactca tgaccgggac gggtaatcca tctgcggcaa ttaatatggt
     7981 tcgaaaacac gcgacctgtc gtgaatttaa aggcgatgtc tcggcggaat acggtagctg
     8041 gaacaaagaa cggtatgtgg cggatttaca aagcccactc accgaagacg gtaaaatccg
     8101 cgcgcgaatt gtcggcggct accagaataa cgactcatgg ctggaccgct acaatagtga
     8161 aaagaccttc ttctcgggca ttgtcgatgc tgatttaggc gatcttacga cgctttcagc
     8221 cggttacgaa tatcagcgca ttgatgttaa tagcccaacc tggggcggtt taccgcgctg
     8281 gaatactgat ggcagcagca acagttacga tcgcgcacgc agtaccgcac ctgactgggc
     8341 gtacaacgat aaagagatca acaaggtctt tatgaccctg aagcagcggt ttgctgatac
     8401 ctggcaagcg acactgaatg ccacccactc tgaagtcgaa tttgacagca aaatgatgta
     8461 tgtcgatgcc tatgtaaaca aagcggatgg tatgctggtt gggccataca gtaattatgg
     8521 acctggcttt gatcatgtcg gcggcaccgg ttggaacagt ggcaaacgta aagttgatgc
     8581 gctggatttg ttcgctgacg gtagttatga attgtttggt cgtcagcaca atctaatgtt
     8641 tggtggcagt tacagcaaac aaaacaatcg ttcagttcat gggccaacat cttcccggat
     8701 gaaattggca gtttctacaa ctttaatggc aatttcccac aagccgactg gtcatcacag
     8761 agcctggcgc aggacgatac cacacatatg aaatcgttat atgctgccac tcgtgtcacc
     8821 cttgccgatc cgctgcatct gatcctcggc gcacgttata ccaactggcg ggttgatacg
     8881 ctgacttaca gcatggagaa aaaccacacc acgccttacg ctggtctggt gtttgacatc
     8941 aatgacaact ggtcgaccta cgccagctat acctctattt tccagccgca aaatgatcgt
     9001 gacagttcag gcaaatatct ggctccaatc accggtaaca actacgagct gggtctgaaa
     9061 tcggactgga tgaatagccg tctgaccacc acgttagcca tcttccgtat tgagcaggat
     9121 aatgtcgctc agtccaccgg tacacctatc cccggcagca acggcgaaac cgcctataaa
     9181 gcggtggatg ggacagtcag taaaggggtg gaatttgaac tcaacggcgc aattaccgac
     9241 aactggcagc tgacatttgg cgcaacgcgc tatcttgcag aggataacga aggaaacgcc
     9301 gttaatccta atctgccacg caccacggtt aaaatgttca ccagctatcg gttgcctgtc
     9361 atgccagagt tgacagtcgg cggtggtgtt aactggcaaa atcgcgtgta taccgacacc
     9421 gtgacaccgt atggcacctt ccgcgccgag caaggtagct acgcgctggt ggatctcttc
     9481 acccgctacc aggtgacgaa aaacttctcg ttacagggga acgtcaataa cctgttcgac
     9541 aaaacctacg ataccaacat ggaaggttct atcgtctacg gcgcaccgcg taatttcagc
     9601 attaccggca cgtatcaatt ctgaataaca cctgtaaaaa aggcagccat ctggctgcct
     9661 tagtctcccc aacgtcttac ggattagtgg ttacggatgt actcatccat ctcggttttc
     9721 aggttatcgg atttagtacc gaaaatagcc tgaacaccag aaccagcaac cactacgccc
     9781 gctgcgccca gtttcttcag gccagcctga tccactttag acacatcagc aacgctgacg
     9841 cgcagacggg taatacatgc gtcgaggtta gtaatgtttt ctttaccacc aaatgcagca
     9901 accagagccg gtgccatttc gctggtacct gttgcttttg catcttcagt cgcgtcttca
     9961 cgacccggcg ttttcagatc cagtgcttta atcagcacgc ggaagatggt gtagtaaaca
    10021 atcgcataac cgataccgac gatcggtaac agccacagtt tgctgctgtt accagacaga
    10081 acgatgaagt cgatcagacc gtgcgagaac gacgtaccgt cacgcatccc cagaagaata
    10141 cagattggga atgccaggcc tgccagaatc gcgtggatga tgtacaggat cggcgcaacg
    10201 aacatgaaga agaactcgat cggctcggtg ataccggtca ggaacgaggt cagcgccgcg
    10261 gagatcataa taccgcccac tttcgcgcgg ttttctggtt tagcagagtg ccagatagca
    10321 attgcggcag ctggcagacc gtacattttg aacaggaagc caccagacag tttacccgca
    10381 gtcgggtcac ccgccatata acgcggaatg tcgccgtgga aaacctgacc tgctgcgttg
    10441 gtgtattcac caatctgcat ctggaaaggt acgttccaga tgtggtgcag accaaacggt
    10501 accaggcaac gttcgatgaa accgtaaatg ccaaacgcaa ctaccgggtt ctggtaagca
    10561 gcccactgag agaaggtctg gattgcagaa ccaatcggcg gccaaatgaa ggacagcaca
    10621 acgccagtaa agatggcagc caggccagaa atgatcggca caaagcgttt accggcaaag
    10681 aagccaagat actcaggcag cttaatacgg tagaaacggt taaacatgta cgctgcaatc
    10741 gcaccggaga taatccctcc gagtacgcca gtatccgcca agtgtttaga ggcgatttct
    10801 tcagcaggta aatgcagtac cagtggcgca accacggcca tggttttaac catgatgcca
    10861 taggcaacaa ctgcggccag cgcggatacg ccatcgttat tggtaaagcc gagggcgaca
    10921 ccgatcgcaa aaatcagtgg catgtttgca aagacggaac cgcctgcttc tgccataaca
    10981 tgcgatacaa cggcgggcag ccagctgaaa ttcgcggaac cgacgcccag cagaatacct
    11041 gcgataggta gtacggatac cggcagcatc agcgatttac cgaccttttg caggttagca
    11101 aatgcattct taaacataat tgagagtgct cctgagtatg ggtgcttttt ttacgttctc
    11161 acgcgtggca aggggggaga gcctcgccgt gtacagggca tctaagcgcc ctttatttat
    11221 tacacagagt aaaataattc agtgccaatt tgtttgacgg ctatcacgtt tcaacttcag
    11281 atcattggag aagttgcaca ttttgacaaa acatatgtca caatatttca aacgcggtta
    11341 ttcaccgccc aattttcggc ggtgaacttt actcgtttta gccattaatt acgagcttta
    11401 aaaaaactca ttcaacggat ggattgaagg cgggaagcgt cgatgtgaaa cagacgggcg
    11461 aagttatcgg tggttacctg cgccagttct tcaacggcaa cacctttcaa cacagccatg
    11521 tattctgcaa cgtcacgaac catcgcaggt tgattctctt ttcctcgatg cggtaccggc
    11581 gcaaggtaag gtgagtcagt ttccaccagt aatcgatcca gggggacata acgcgcagca
    11641 tcgcgcagtt gctccgcatt acggaaggtc acaatgccgg aaaaggagat gtaaaatccg
    11701 agatccagta atttacccgc cgtttctctg tcctctgtaa aacagtgtag tacgccaccg
    11761 caatccgtca ctttttcttc gcgcagaatt gccagcgtat cggcacgggc gtcacgggta
    11821 tggacgataa ccggcttgtt cagttcacga ccaatctgga tatgatggat gaaggactct
    11881 tgctgacgta ctttagtttc cggtgtgtaa taataatcca gcccggtttc acccagcgct
    11941 acaacaccct cttctgccgc cagacggcgt aaatcttcta catcgtaggg atcattctgg
    12001 ttgagtggat gcacgccaca agaaaatacg acgttgtcac gttcgcctac cagatcccgc
    12061 atatgtaaat aacccggtaa tgttgtggcg actgccagac aaaatttcac atcgcgtgcg
    12121 gcggctttcg ccagaacgtc atccacgtcc ttatgcaaag attcataatc cagaccatcg
    12181 agatggcagt gtgagtcgac taaaaacatg atgtctctct taaagatgag gaaccggtaa
    12241 cacaacgccc ggttgcaggt aatgctcaat acgcagtaaa agatcggtga tgagaagctc
    12301 gcggttgatg cctgtgacag acattaactg ttcacgaatg tggcaaacat cccccagtat
    12361 agcctgcagg cacgagggag aaagatggtt tgccagttcg gcgaccaggc ccggcacatc
    12421 aacattggtc acctgcgcag cgccatgatg gcgttttagc gcatccatca gcaacgttgc
    12481 cagccagtgt aaacgcgccg gagcttgttc atgattaagg gccgccagca gcgaatacca
    12541 gtcgcccgat ggcacgctat atgccaacgc ctgacacaat gtttcacgag cctgccagtt
    12601 atctccctga aacaacgcca gtgccgcgcc aggcgaaccg gcgcttaagc gcaatgcggc
    12661 aagtaatgca tcctgtgaca ttgtcacttc gcgtgaaagc caagtcacgg cgtactgttc
    12721 tggaggcggc gcaaggtaat gtaaccgaca acgactacgt aatgttgcca gtaaacgttc
    12781 aggctcgcgg gtagccagga aaaaccaggt ttctgctggt ggctcttcaa gcgttttcag
    12841 caatgcgtta gccgcggcgt cggtcagtaa ggcagcatcg gttacccaga caactttcgc
    12901 accacctagg cgtgcgtgct cattcagctt ttcggtgacc tcacgtaccg catcaatgcc
    12961 cagcgtattt ttcccttttt cgggagccag ggtgtagtaa tcgggatgcg tgccagcctg
    13021 catcaactga catccacgac agtgaccgca acttttgtgg ccctgcggtt gttggcagag
    13081 taaataacgg ctcagggcgt agattaaagc atcatcgccc atgcccggta acgcctgaat
    13141 gagtagcgca tggtgacctc ttccggcctg atagctggct accagttttt cgaaatcagg
    13201 tcgtaaccat ggataccatc tcatgcgtcc aactccttca cccagtgggt cacggtagtg
    13261 cggattgcat ccatcacggc ctccagcggc tgggtggcat caatggtatg aatactttta
    13321 tcttgtgcag ccagttccag atagcgggcg cgggtgcgat taaagaaatc gaaagactct
    13381 tgctcaatac gatccagctc gccgcgcgca cgcgcgcgtt ttaagccaac ttccggggta
    13441 acatcgagat agagcgttaa gtctgggcga aaatccccga gaacagcatc acgcagtgtt
    13501 gccagcatat gttggtcaat accacgtcct ccgccctgat acgcctgagt ggaaagatcg
    13561 tggcgatcgc caatcaccca ggtgccgtta gccagcgcag gtttgatgac cgtttctacc
    13621 agttgaacgc gcgcggcata aaacatcaga acttcggctt tatcggtaat gacttcatcg
    13681 cctaccgatt tgatatccag caccaggctt cttaactttt cggcaagttg cgtaccgcca
    13741 ggttcccgag tgaaaaccat gtcgcggata cccagttgct cgagcgtctc aaccaccaca
    13801 ttacgcgcgg tagttttgcc tgcgccttcc agcccctcaa tgacgatata cttactgcgc
    13861 atttttttcc ttaagcactt tcagataatc ctgcacagac ttgttatgac tggcaagatt
    13921 ggtattaaac gtgtgaccac ctttaccatc ggccacaaaa tagagatacg gcgtttttgc
    13981 cggatgcgca gcagccttca gcgaatccgc ccccggcgta gctatcgcac ccggcggcaa
    14041 accggtaatg gtataggtgt tatacgctgt cggcgtttcc aggtctgcac gagaaagttt
    14101 gccattataa cgctctccca tcccgtaaat cacggttggg tcggtctgca ggcgcatacc
    14161 aatgcgtaaa cggttgataa ataccgaggc aacctgatcg cgttcactgg caacggcggt
    14221 ttctttttcg ataattgatg ccatcgtcac cagctgattt ttatctttat aaggcagacc
    14281 gtccgcacgc ccttcccagg cgctatcgac cgctttcacc attttcttgt gaactcgctt
    14341 gagtaacgcg acatcggtgg tattggcggt atacatcaag gtgtctggcc agaaccaacc
    14401 ttcaatccac tccgggtttt ccagttcaag tgcctgcgct acggtggcgt acttatcgtc
    14461 actcagcgtg tgcttgatat acggagcctc acgcaattgc ttgaggtagt cgctcagacg
    14521 catcccttct accagtcgca gcgggaactg tgcttcttta ccgctttcca acaatttcag
    14581 catctcgcgc acggtcatct gcggtgtaaa gcggtaagtc ccggctttaa agtgagaaag
    14641 atccggttcg atacgcagca gccattgaaa aacccgtggg cgattgatga tcttatcagc
    14701 ataaagctgt tcaccgagcg ccagacgtcc ggtccctggc ttcagggtaa atatcgtctc
    14761 ttctttgata agcaatttgc tgtcggcaag atggcgaacc ttccagacgc ccacaccagc
    14821 ggcgataccc agtaccacca gcaataacaa gataatcaat aacacttttt tcatgactaa
    14881 ttcgggcgct cacaaagtgg ggctaaatat tcatataacg ttgctgacga aaaggtgaca
    14941 tcgccacagg cacgtacggg catcactggc attaacgcat tacaaataac catctcatct
    15001 gcctgcaacg cctcctccag cgaggcttgc acttcgacaa gctgataaga ggattgtgcc
    15061 agtaaacgga tacagaattg tcgcataatg ccgttaacac ctgcctgatc cagtcgcggc
    15121 gtataaacta cattgccctt ccgccagaac aaattagccg cacagcattc cgtaacccac
    15181 ccttcgctgt caaggaccag cgcctcatca gcgtttgtct gctcaagatg agagcgaatc
    15241 aatacttgct caagccgatt aagatgttta atacctgcaa gatgaggatt gcgccccagc
    15301 cgcaccgggc ttagcgccaa cgtcatccct tcgttacgca aacggtcgta atgtgcagga
    15361 taagccgtaa cggagagaat ccgcgttgct ggtccgctgt tcaatgtgct gtaccctcgc
    15421 ccgccactac cgcgactgat cacgactttc agcacaccat tttgctgttc tgctgccagc
    15481 gttttcatct cttgttcaag ctgaggccag aagtcacagg aaatcatcaa ccgctgacaa
    15541 gcatcctgta gtcgcaggat atgcgccgat aacaaactga ctttaccgtc gataactctg
    15601 gcggtggtaa aacaaccatc accaaactgc gttgcccgat cgcttactgc cagcgattcc
    15661 tgcttataac cgttaattaa gaacataccg gctccttatc agagaattgt tagtgtggca
    15721 gcatgttcac tacggaacaa gtcggaataa aagctaagaa aaaaggcccg caagcggacc
    15781 ttttatatgg gtggaaaatg acaacttaga tctttttaaa gatcaaagaa ccattagtgc
    15841 caccgaagcc gaaggagtta cacagcgtgt attccattcc gctgacctga cgtgcttcat
    15901 gcggtacgaa atccagatcg caaccttcat ccgggttatc caggttgatg gttggcggaa
    15961 cagcctgatc gcgcagcgcc aggatggagt agatagattc tactgcaccc gccgcaccta
    16021 acaggtgacc ggtcatagat ttcgtggagc ttaccaacac acggcttgca gcttcaccga
    16081 agatggtttt caccgcctgc gcttcagctt tatcgccagc cggcgtagaa gtaccgtgcg
    16141 cgttaacgta gccaatctga ctcgcttcaa tgcctgcatc acgcagagca tttgccattg
    16201 ccagagctgc gcctgcgcca ttttctggcg gtgacgtcat atgataagca tcgctgctca
    16261 taccaaagcc gacgagttca gcgtaaattt tcgcaccgcg ttttttcgcg tgttcgtact
    16321 cttcaagtac cagcataccg gcaccatcgc ccagtacgaa gccatcacgc tctttatccc
    16381 acgggcggct cgccgcttgc gggttatcat tgcgggtaga taatgcacgt gccgcgccaa
    16441 aaccaccaac gcccagcggc gtactggctt tctctgcgcc acctgcaacc atcacgtcag
    16501 catcgccata cgcgataata cgcgcagcat ggccaatgtt gtgcacgccg gaagtacagg
    16561 cggtcgcgat agagatgctt gggccacgca ggccatacat gatagtcaga tgacctgcca
    16621 ccatgttcac aatcgttgac ggaacgaaga atgggctgat cttacgtgga ccaccgttca
    16681 tcagagatgt gtggttttct tcgatcagtc cgaggccgcc aatcccggag ccaattgcgg
    16741 caccaatgcg ggttgcgttc tcttccgtta tttcaaggcc agaatcctgc atggcctgaa
    16801 cgccagcgac aattccatat tgaatgaagg catccatctt gcgctgttct ttgcgcgaga
    16861 taatgtcctc acagttaaaa tcctttacta agccagcaaa tttcgttgca taggcgctag
    16921 tatcgaaatg gtcgattagg ctgatgccac tctgaccggc aagcagagct ttccaggtag
    16981 actctacggt attgccgaca ggagacaaca tgcccagtcc ggtcacaact acacgacgct
    17041 tagacacgtt tgtcctccaa ggagggaaaa atgattctag tgggacaaaa agataaaact
    17101 caggcggtcg aacgaccgcc tggagatgtt cacttacgcc tggtggccgt tgatgtaatc
    17161 aatggcagcc tgaacggtgg tgattttctc agcttcttcg tccggaatct cagtatcaaa
    17221 ctcttcttcc agagccatta ccagctcaac ggtgtcaaga gaatccgcgc ccaggtcttc
    17281 aacgaaagaa gcattgttgg taacttcttc ctgcttaacg cccagctgtt cgccgataat
    17341 tttcttaacg cgttcttcga tagtgctcat actcttaaat ttcctatcaa aactcgcttt
    17401 cgcgatggtt ttcgtagtgt ataaaatgtt gaaaaatttg caactaaatc ccggcaggtc
    17461 ttaccacgat tttacgttat tttgaggcct taccccccaa taacgcaaat cattttgcac
    17521 taattattac tcataaccac gcagactgcg cgcaacatga gcattttgtg caaatcgcgg
    17581 tcagaccatg tacatcccgc cgttcacatg caaagtttca cccgtgatgt aagctgcttc
    17641 gtcggatgcc aggaatgcaa ccgcgttggc gatttcctgt gcgccgccga ggcgacccgc
    17701 aggaacctgc gccaggatac ccgcacgctg gtcatcgctc agcgcacgtg tcatgtccgt
    17761 ttcaataaag cccggagcaa caacgtttac agtaataccg cgtgacgcaa cttcgcgcgc
    17821 cagtgattta ctgaagccga tcaagcccgc tttcgccgca gcgtagttgg cctgaccgcc
    17881 atttcccatg gtaccaacca cagaaccgat agtgataata cgaccatgac gctttttcat
    17941 catagcgcgc attaccgctt ttgacagacg gaaaacagat gaaaggttag tttcgataat
    18001 atcgttccac tcttcatctt tcattcgcat taacaggtta tcacgagtga taccggcatt
    18061 attgaccagg atatccactt caccaaattc tgcgcgaatt ttttccagaa cagattcgat
    18121 agatgccggg tcggtcacat tcaacatcag acctttgccg ttggcaccta aataatcact
    18181 gatcgcctga gcgccatttt cactggtcgc agtgccaata actttcgcgc cacgggctgc
    18241 gagcgtttca gcaattgcgc ggccaattcc gcggcttgca ccggttacca gtgcgatttt
    18301 tccttcaaaa ttcatgattt tcctctttta aagctcgagc gccgctgcca tcgctgaagg
    18361 ttcgttcagc gccgaggcgg tcagggtgtc gacaatgcgt ttcgtcaggc cagtaagcac
    18421 cttgcccggg ccgacttcat agagatgttc tacgccttgc gctgccatgt actcaacaga
    18481 cttcgtccac tgaaccgggt tatacaactg acgtaccagt gcgtcacgga tggcatcacc
    18541 attggtttcg catttcacat caacgttatt cacaacagaa actgttggtg cgttaaaggt
    18601 gattttcgct aattctactg ccagtttgtc ggctgctggt ttcatcagcg cacagtgaga
    18661 cggtacgctc actggtaacg gcagcgcgcg ttttgcgcca gcagctttac aggcagcgcc
    18721 agcacgctca accgcttctt tatgaccggc aataaccacc tgtcccggag agttaaagtt
    18781 taccggagaa acgacctgac cttctgcagc ttcttcacac gctttcgcaa taggcgcatc
    18841 atccagaccg atgattgccg ccatagcgcc cgtgccttcc ggtacggctt cttgcatgaa
    18901 cttaccgcgc atctcaacca gacgcaccgc atcagcgaaa tcaatcacgc cagcgcaaac
    18961 cagcgcggag tattccccca ggctgtgacc ggccatcatt gccggtgctt taccgccctg
    19021 ctgctgccat acgcgataca gcgcaacaga tgcagtcaac agcgctggct gggtttgcca
    19081 ggttttattc agttcttcag ctgacccctg ctgggtcagc gcccacaggt cgtagcccag
    19141 cgccgcagaa gcttcagcaa acgtttcttc gacaattgga tagctcgccg ccatatcagc
    19201 cagcattcca acggtttgag aaccctgtcc agggaacaca aatgcaaatt gcgtcatgtt
    19261 ttaatcctta tcctagaaac gaaccagcgc agagccccaa gtgaatccac cgccaaaggc
    19321 ttcaagcaga accaactgcc ccggcttaat gcgcccgtcg cgtacagctt catccagcgc
    19381 gcacgggaca gaggccgcag aggtattacc gtggcgatcc agcgtcacca cgacattgtc
    19441 catcgacata ccgagttttt tcgccgttgc actgataata cgcaggttag cctgatgcgg
    19501 aaccagccag tccagttgag aacggtcaag attattcgcc gccagcgtct catcaacgat
    19561 gtgcgccagt tccgttaccg caaccttgaa gacttcgttg cccgccatcg tcagatgaat
    19621 tgaattctct ggattcacgc ggtcggcgtt tggcagcgtc agcaattcac cataactacc
    19681 gtcggcatgc agatgggtgg aaatgattcc cggctcttca gaggcagcca gcaccgcagc
    19741 gcccgcgcca tcgccaaaaa taataatagt cccacgatct gttggatcgc aggtgcgcgc
    19801 cagtacatcg gaaccgacga ccagagcata cttcaccgcc ccagatttca cgtattgatc
    19861 ggctacgctt aatgcatagg tgaaacctgc acaggctgct gcaacgtcaa atgccgggca
    19921 acctttaatg tccaacatgc tttgaatctg acaagctgcg ctcgggaaag cgtgcgtagc
    19981 agaagtcgtt gccacaacga tcaggccaat ctggtctttc tcaatgcccg ccatctcaat
    20041 tgcgcgtgtc gccgcttcaa agcccatggt tgaaacggtt tcgtttggcg cggcaatgtg
    20101 gcgttcgcgg ataccggtac gagtgacaat ccactcgtca gaggtgtcca ccattttttc
    20161 caaatcggcg tttgtccgca cttgttcggg cagatagctg ccagtaccaa taatcttcgt
    20221 atacatgtac gctcagtcac ttttcggtta tataccgtca cttgcaaact gcgagttcgc
    20281 tggcagcgtc ctgctaccgc agagttccgc ttttgccacc gtccagcagc tcaaaaccag
    20341 ctgggtatac agattccagg cgagcggcaa ttcgctgagg aacttgtcgc tgcaccgcct
    20401 gcactgcctg ttcaatcgcg accgcaaaag ctcgctgatt ggctgcacca tgacttttta
    20461 tcaccgtgcc gcgcaatcct aacagacagg cgccgttata ctggtcgggg ttgaggtgac
    20521 tgaatcgcct cgtcaggctc ttttgtagcc aacgctttaa taacagtagc caccacgacc
    20581 gttttttccc ttcaccctga gatttcagca gagaaaggaa catcctgaca acaccttcca
    20641 tcgtctttaa cgtgacattt cctgtaaagc cgtcacaaac cagcacatct gtcttgccag
    20701 ttaacaactc attggcttca agatagccga tataattgat agaagggatt gttttaagca
    20761 ccgctgaggc atcccgaata ctgtcaagac cctttacttc ttcttcacca atattgagca
    20821 acgccacgcg aggattggga atttccacca cctcttcagc cagaactgag cccataatgg
    20881 caaattgcac caacattgtg ctgtcacaat cgacgttggc ccctaaatcg aggaccaccg
    20941 ttttgccctt ttgctgatgt ggtaataccg tcaccagcgc cggacgctca atcccctcca
    21001 ggggcttgag taataatttt gccagcccca tcagtgcccc ggtattaccg gcactgacac
    21061 aggcttgcgc tcgaccttct ttcaccagct ccagggccat acgcattgaa ctcccacggc
    21121 tggcgcggat agcttgcgaa ggccgggcat cactggcgat aactgactgc gcaggaataa
    21181 tctgcagacg cgaacgttgt tcaaagtcag ctttagcaag taatggcgtg atggcgtcgg
    21241 gattgccgac taaaagaaga gtgagttgcg aattagaatt cagtgcctgc aatgctgcag
    21301 gcactgtcac ggaagggcca aaatcccctc ccatgacatc taacgccagg gttagacgtg
    21361 tcaaggtatc gtcgccgccc ggtttggtct ttccccagtt gcctggggaa aatcctcact
    21421 aagcttcatc acgcagacgc gtgattactt agcgatgacc ttgcggccgc ggtagtaacc
    21481 gtcggcagtg atgtggtgac gcaggtgttt ttcaccagaa gttttgtcta cagacaggct
    21541 ggtgactgcg gtcagcgcgt catgggaacg acgcatgcca cgtttggaac gggttggttt
    21601 attctgttgt acggccatgg accttactcc tcaattacgg tttatccccg atccaacggg
    21661 gagcaccaat tacttacgct ttaagctggc taatacggca aatgggtttg gcttttgcac
    21721 ttcttcaggc agttcaccaa agaccatgtc cgcttcggac acttcacagt gttcagaatc
    21781 atgcaccgga actaccggca aggcgaggat gatttcatct tcaaccattg caagcagatc
    21841 gatttcaccg aattcgttaa cctcaatcgg ttcatacgct tccggcagtg cttcagcctg
    21901 ttcgtctgaa cgcacaggac taaaacaata cgttgtgtag acctgatgag taaacggctt
    21961 cccgcaacgc tgacactcga gcgttaccgt caccttcgca tcgccgttta acactgcgag
    22021 acgttggtta tcgatagcga acgacatgga gcattccaca tcactgtcca cactgactac
    22081 ggattcggcg acgcgctcaa cctgatcagg ggtatagata ccctggtaat caaggcgttt
    22141 ttgagccgta cgaaccggat cgagagtcag gggtaatttt accttttgca tagggcgcgc
    22201 atattaactt tgtaacgtca tagagtcaaa gaaaaaggca gcctgtggtt gccttttgcc
    22261 aataattcgc acacattgcg ggttactgct ttattttcgt tcagtgctga taacgaacat
    22321 caacctgcaa aacgaagcgc atagtttaaa atggctatca tcaatacgct atatctgatg
    22381 gaaaaaaata tgcctaaact tattttagcc tccacctcgc catggcgtcg cgctcttctg
    22441 gaaaaactgc aaatctcttt tgaatgtgca gcacctgagg tcgacgaaac cccacgcagc
    22501 gacgaatcac cgcgacagtt ggtgcttcga ctggcacaag aaaaatcgca atctctggcg
    22561 tcacgttatc cggatcattt aattattggt tcagaccagg tatgtgttct tgatggcgaa
    22621 atcaccggta aaccgttaac ggaagaaaat gcccgtctgc aattacgcaa agccagcggc
    22681 aatatcgtca ccttctatac tggactggcg ttgtttaatt cggcgaatgg gcatctacaa
    22741 acagaagtgg agccttttga cgtccatttc cgtcacctaa gcgaggcgga gattgataat
    22801 tacgtgcgta aggagcatcc cctgcactgc gcgggtagct ttaagagtga aggatttggc
    22861 attacgctgt ttgagcgttt agagggacgt gaccctaaca cgctggttgg tttaccgcta
    22921 atcgcgctgt gtcagatgtt acgtcgggaa gggaaaaacc cgctgatggg atagttgttt
    22981 gcgccggatg cggttacgtc gcatccggca atcggtgcag gcggcctgat gcggcgtaaa
    23041 cgccttatca ggccaaccgc ccattatcag gcttatatgc ttgtattagc gcgcgttacg
    23101 cagcttttgc aaacaacgct tcaaaccttc atccatcggc gcttcgatac gcatcacctc
    23161 accggtcccc ggatgggtaa acttcaacgc tgcggcgtgc aggaacagac gatttaatcc
    23221 cgtgcctgct tcagtgagct gtctgtcaaa ttcacggtca ccgtagcgat cgtcaaaggc
    23281 aatcggatga cccgcatact gtgtatgcac acggatctga tgagtacgtc cggttaccgg
    23341 gctacaacgc accagggtgg caaatgcata gcgttcttcc actttaaagc gtgtttccga
    23401 cggtttgcct tcctgactga cacgcacgat acgttcgccg ctttgcagaa tatttttcaa
    23461 taacggcgct tgaacgctct tcacatgcga ctgccactga ccgcgcacca gcgccaggta
    23521 atctttttgc attccttttt cacgtaattg ctcatgcaga gaacgcaacg ccgagcgttt
    23581 tttcgctacc agcaacacac ctgaagtgtc ccggtcgaga cgatgaacca gttcaaggaa
    23641 ccgcgcttcc gggcgcaacg cccgcaaacc ttcgataacg ccgaagctta aaccactgcc
    23701 gccatgtacc gccgtaccgg aaggtttatt cagcaccagg atatgatcat cttcatataa
    23761 gatgacgtcc gccagcgccg ccaccttttg cagatgtggc gaaaccgcct cttcttcccg
    23821 ctcagcaacg cgaaccggtg ggatacgcac ctcatcaccc gcttcgagtt tatattcagg
    23881 cttaatacgt tttttgttca cccgcacttc acctttacgc aaaatacggt aaatcatact
    23941 ttttggtacg cctttcaatt gggtacgcaa aaagttatcg atacgttgcc ccgcttcgtc
    24001 agcggtgata gcaacaattt ttacggatgg agtctctgtt ttcatggtgg gcgattctaa
    24061 ttagccaaca ggattcgcgc cactcatttt tctatgctta tttttagttt tgcaccttat
    24121 tacttcacta cgtgatcact ttattgatgg ttattaaacc aatcaccagc aagaagtgaa
    24181 aaaactgtga gtaagcgggt gataaatggt aaaagtcatc ttgctataac aaggcttgca
    24241 gtggaataat gaggccgttt ccgtgtccat ccttgttaaa acaagaaatt ttacggaata
    24301 acccattttg cccgaccgat catccacgca gcaatggcgt aagacgtatt gatctttcag
    24361 gcagttagcg ggctgcgggt tgcagtcctt accggtagat ggaaatattt ctggagagta
    24421 atacccagtc tgtttctttg ataattgcgc tgtttttccg catgaaaaac gggcaaccga
    24481 cactctgcgc ctctttgagc tgacgataac cgtgaggttg gcgacgcgac tagacacgag
    24541 gccatcggtt cacacccgga aaggcgttac tttgcccgca gcttagtcgt caatgtaaga
    24601 ataatgagta agttacgatg aaaagaatgt taatcaacgc aactcagcag gaagagttgc
    24661 gcgttgccct tgtagatggg cagcgtctgt atgacctgga tatcgaaagt ccagggcacg
    24721 agcagaaaaa ggcaaacatc tacaaaggta aaatcacccg cattgaaccg agtctggaag
    24781 ctgcttttgt tgattacggc gctgaacgtc acggtttcct cccactaaaa gaaattgccc
    24841 gcgaatattt ccctgctaac tacagtgctc atggtcgtcc caacattaaa gatgtgttgc
    24901 gtgaaggtca ggaagtcatt gttcagatcg ataaagaaga gcgcggcaac aaaggcgcgg
    24961 cattaaccac ctttatcagt ctggcgggta gctatctggt tctgatgcca aataacccgc
    25021 gcgcaggtgg catttctcgc cgtatcgaag gcgatgaccg taccgaatta aaagaagcac
    25081 tggcaagcct tgaactgccg gaaggcatgg ggcttatcgt gcgcaccgct ggcgtaggca
    25141 aatctgctga ggcgctgcaa tgggatttaa gcttccgcct gaaacactgg gaagccatca
    25201 aaaaagccgc tgaaagccgc ccggctccgt tcctgattca tcaggagagc aacgtaatcg
    25261 ttcgcgcctt ccgcgattat ttacgtcagg atatcggcga aatccttatc gataacccga
    25321 aagtcctcga actggcacgt cagcatatcg ctgcattagg tcgcccggat ttcagcagca
    25381 aaatcaaact gtacaccggc gagatcccgc tgttcagcca ctaccagatc gagtcgcaga
    25441 tcgagtccgc cttccagcgt gaagtccgtc tgccatccgg tggttccatt gttatcgaca
    25501 gcaccgaagc gttaacagcc atcgacatca actccgcacg cgcgacccgc ggcggcgata
    25561 tcgaagaaac tgcgtttaac accaacctcg aagccgccga tgagattgct cgtcagctgc
    25621 gcctgcgtga cctcggcggc ctgattgtta tcgacttcat cgacatgacg ccagtacgcc
    25681 accagcgtgc ggtagaaaac cgtctgcgtg aagcggtgcg tcaggaccgt gcgcgtattc
    25741 aaatcagcca tatttctcgc tttggcctgc tggaaatgtc ccgccagcgc ctgagcccat
    25801 cactgggtga atccagccat cacgtctgcc cgcgctgctc cggtaccggt accgtgcgtg
    25861 acaacgaatc gctgtcgctc tctattctgc gtttgatcga agaagaagcg ctgaaagaga
    25921 acacccagga agttcacgcc attgttcctg tgccaatcgc ttcttatctg ctgaatgaaa
    25981 aacgttctgc ggtgaatgcg attgaaacgc gtcaggacgg tgttcgctgc gtgattgtgc
    26041 caaacgatca gatggaaacc ccgcactacc acgtgttgcg cgtgcgcaaa ggggaagaaa
    26101 cgccgacctt aagctacatg ctgccgaagc tgcatgaaga agcgatggcg ctgccgtctg
    26161 aagaagagtt cgctgaacgt aagcgtcctg aacaacctgc actggcaacc tttgccatgc
    26221 cggatgtacc gccagcgcca accccagctg aacctgccgc gactgtcgta gccccagcac
    26281 cgaaagctgc aacggcaaca ccagcagctc ctgcacaacc tgggctgtta agccgcttct
    26341 tcggcgcact gaaagcgctg ttcagcggtg gtgaagaagc caaaccgacc gagcaaccaa
    26401 caccgaaagc agaagcgaaa ccggaacgtc aacaggatcg tcgcaaacct cgtcagagca
    26461 accgccgtga ccgtaatgag cgccgcgaca cccgtagtga acgtactgaa ggcagcgata
    26521 atcgcgaaga aaaccgtcgt aatcgtcgcc aggcacagca gcaaactgcc gagacgcgtg
    26581 agagccgtca gcaggctgag gtaacggaaa aagcacgtac caccgacgag cagcaagtgc
    26641 cgcgtcgtga acgtagccgc cgccgtaacg atgataaacg tcaggcgcaa caagaagcga
    26701 aggcgctgaa tgttgaagag caatctgttc aggaaaccga acaggaagaa cgtgtacgtc
    26761 cggttcagcc gcgtcgtaaa cagcgtcagc tcaatcagaa agtgcgttac gagcaaagcg
    26821 tagccgaaga agcggtagtc gcaccggtgg ttgaagaaac tgccgctgcc gaaccaattg
    26881 ttcaggaagc tccagctcca cgcacagaac tggtgaaagt cccgctgcca gtcgtagcgc
    26941 aaactgcacc agaacagcaa gaagagaaca atgctgataa ccgtgacaac ggtggcatgc
    27001 cgcgtcgttc tcgccgctcg cctcgtcacc tgcgcgtgag tggtcagcgt cgtcgtcgct
    27061 atcgtgacga gcgttatcca acccagtcgc caatgccgtt gaccgtagcg tgcgcgtctc
    27121 cggaactggc ctctggcaaa gtctggatcc gctatccaat tgtacgtccg caagatgtac
    27181 aagttgaaga gcagcgcgaa caggaagaag tacaagtgca gccgatggtg actgaggtcc
    27241 ctgtcgccgc cgctgtcgaa cctgttgtta gcgcgccagt tgttgaagaa atggctgaag
    27301 tcgtagaagc ccccgtaccg gttgccgaac cgcaaccgga agtggttgaa acgacgcatc
    27361 ctgaagtgat tgccgccgcg gtaactgaac agccgcaggt gattaccgag tctgatgttg
    27421 ccgtagccca ggaagtcgca gaacacgcag aaccgatggt tgaaccgcag gaagagacgg
    27481 cagatgcaga tattgaagaa gttgccgaaa ctgctgaggt tgtggttgct gaacctgaag
    27541 ttgttgctca acctgccgcg ccagtcgtcg ctgaagtcgc agcagaagtt gaaacggtgg
    27601 ccgcggttga accagagatc accgttgagc ataaccacgc taccgcgcca atgacgcgcg
    27661 ctccggcacc ggaatatgtt ccggaggcac cgcgtcacag tgactggcag cgccctactt
    27721 ttgccttcga aggtaaaggt gccgcaggtg gtcatacggc aacgcatcat gcctctgccg
    27781 ctcctgcgcg tccgcaacct gttgagtaat aattagctca aagtaatcaa gccctggtaa
    27841 ctgccagggc ttttttattt catgtttgaa tcaacatatt aaaattcatc aaatggcatc
    27901 cttgctaacc aacaatgcaa aataggcaaa aaaaatgccc gccccggcga tcccagagcg
    27961 ggcagacaaa acatacccag tttcatgata tgttttaaag cgagcttatt tgttgagctg
    28021 gaagagcgac aatccctgca tatcggtaaa tgctttatac gatgcctgca atgccgtttg
    28081 ctgcatgatg taagatgaaa tagttgcatt ccagtccaca tcaaccagat cgctcatctg
    28141 ctgcgtttgc cctaaagcgc gatcgctacc taaggaatcc agcgactcca gtttgttcag
    28201 ctgcgtgcct aattccgcgc gaacggtaag gacattattc agcgagtttt tcagtccacg
    28261 gttggtttta tccaatgccg cagcggcgat ttctttatcc gcttcgctat ccgcgaccgg
    28321 cgttttcagg gctgcgatgg cgctatccag catggcaaaa agattggttt cagaagcgct
    28381 accgtctggt tccgctaccg cgttactggt aatactgtcg aaaattttgt cgcccgtatg
    28441 tcctatcacc atcgaacgcg aagcatcgac ctgttgctta atactttctg ttccgccaac
    28501 ataatcaccg ttagcctcac taaacggcgc agtctctgtt ttatatgtag cgcccattac
    28561 cgtcagtggt gttcgccaga ttcagcaact ggtcacgaag cccctgaata tccgtagcca
    28621 gcgaggcccg gtcatcgtca ctcaaggtgc cattgctggc gtagacaatt ttttcctgag
    28681 cattctggat agcagtggtg acctggctaa gtacactctc ttccagtgac actttttgag
    28741 tggcgaaagt acgtgccagc gtgtactggc tgttttgcgc ctgtgcctgg gagagaacaa
    28801 cagcttgtga tgcagcaatg ggatcgtctg aagggttaac gactcgctta cccgtcgaca
    28861 tctgctcgcc gtacttcatc cattctgcct gagaattggt gatgccgcgc atgttttgct
    28921 ggtacatcat ctgggtactg aaacgcattt tatctcccct tagcgaatgt taatcagcgc
    28981 atcaaaaatc gcgtttgccg tctgcagaac ctgcgcattc gccaggtaat actgctgaaa
    29041 acgttgcaga tttccgtact cctcatcgag attgacaccg gaaatcgact gctgctgatt
    29101 ggaaagctgc gtcaccacat taccttgcgt ggtgctactg gttttcaacg tcgcggtttt
    29161 attaccgata tcactcacta acgaggcata agcgtcgtta aaggatttcg caccgcccac
    29221 cgttttactg ttgctttgca gatccagcag ggcctgaccg ttgcggttgt cgctatcacc
    29281 cgcatcttct tcgctcgcca tcgcgatttt cgcttcgtcg gtgattaata catccatgtt
    29341 gacgatggcg tcacttactg gtttcagcgt gaagctgtcg ttaacggcag gcgttcctgt
    29401 aaacgtcaac tccagaccat caaatgccac tttaccgttg gcatctggtg tcgccgtaaa
    29461 agtggtattg ctggcaaggc gggtgacctg ccactgatta ttatcgaacg agattttgta
    29521 atctgtcgcc agtaccacgg aggcatcagt taccgtggca ccgatcgcaa cgtcaccttt
    29581 gtttttcgtg ttttgcagaa ccgcgggctt accgatagta aagaaatctt caccagcatc
    29641 gccgttagca tcaaatccgg ctttgtgttg gctgttgaaa gcctcggcaa atgccagcgc
    29701 cagttgtcca agcgtattac gcgtctggtc cagatcctga gaacggaatg tcagaatgcc
    29761 gcccagcgac ccggtattca gtaatttctc cgggatctca atattgcctg ccgtcccatc
    29821 aacgtaagcg acagtcgtac gagaagggtc ggcgctggaa ggaactgccg ccagttgccg
    29881 cgccgtactt ccctgaacca gtgagtaacc attggcatcg tgatgttata agtaccgcca
    29941 tcctgaacgc tgacttctac gccaacaatc tggtttaatt cgctcaccag ttgatcgcgt
    30001 tgatccagca gattgttagg tgacgcccct gcccccacgc ctgtcaggcg cgagatttga
    30061 tcgttcaggc tggcaatttg tttagcgtaa ttgttgatct gatcaacgct ggcacctatc
    30121 gcgatattga cctgtttgtc ctggtcgcgc agatattgat cggtggtttt aaactgattc
    30181 accaaccctt ctgatttccc aatcagcgcc tggcgcgccg ccgggtcttc cgcgttactc
    30241 accagcgttt gcaggctggt gaagaaatcc tgcatctgtg ttgccagcga agaggtactg
    30301 gtggagagca tattgtcgat tttcgacatc tgctcatagc gggcggtcag gccgctactt
    30361 tgcgtctgcg cagcacgtaa ctggttggta ataaacgtat catactcacg ctgcacacca
    30421 gaaacgtaga cgccattgcc aacccagccg ccagcgccca acgtgctatt ggcctgcgcc
    30481 ataatagtgg tttggcgggt atatccggca acgttatagc tggagatatt attacttgcc
    30541 gtatttaacg ccgcctgggc cgcgttcagt ccgctcatgg cgttattgat caagctggac
    30601 atggaggttc cttataagcc ttcaattaca gagtattatc ggcagcgacc cgccggactt
    30661 gagttattca gaacagatta tcaatgttca tgctgtaggt tttgctcacc ttgtcgctta
    30721 tcgatttcat ctgctggatc atgttggtga gtttacgggc atagtgagga tcggtagcat
    30781 agcccgcgtc ctgtaggacc tgcgccccct gttccgcact cgcggcggtc gtcacggcgg
    30841 cgtagcgcgg gttacgtgtt aacagcccaa cgtaatccga caatgcttcc agatacgagc
    30901 tgtagacccg aaacttcgct tttactttct tcgcttcgcc gttttcatat tcagtcgtgg
    30961 tgatttcagt gacttgccct ttccagttgc cagaggcttt gacaccaaac aggttatagc
    31021 ttggctcgcc gttttcgcgg cggatttgcc gttgccccca accagattca agcgcagcct
    31081 gagcgaggat caaatgatgt ggcacaccgc tttgctggct tgccagttgg gcgggcagcg
    31141 agagttgcgc gaggaatgct ctactgtcac ccggcagcga atcatcgtag ttacgtggta
    31201 cggccttttg caccagctgc gaaagcgtct ggttttgata acgcaccacg gtttcgagcg
    31261 ggaatttcat cggtgctgct ggcgtggact cctctggcaa tggttgttct ggcgtcatct
    31321 gtttaaccat catctctgca agccccagac ctttgcccgc cgtcatctgt tgggcaattt
    31381 gctggtcata catactggta tacagacgag tgtgctcgct gctgaacagg ccatcttttg
    31441 gtaaagcgtc gcgcatgctt ttcaacatca tctgcacgaa catcccttcc acctgacggg
    31501 ccaccggacg gatatttgcc gccggatctt cgctcgcttt cgcctttagt tcgttgagtg
    31561 attgcgcatc ccaggccgca cttgccagta gtttgctgtc gctgatcatc agatgatttc
    31621 cagttttgcc cgcagacatc ccgcactttg cattgattgc aaaatagaca tcagatccat
    31681 cggcgtagcg cccagcgcat tgagcgcacg caccacgtta ttgaggctgg cgctggaacg
    31741 tacgctttgc agcgaaccgc cgctctggcg taaatcgatc tgcgtttgtg gagtaacaac
    31801 agtctgtcca ccaccaaacg gtgtatctgg ctggctgaca ttggcctgac gattaactgt
    31861 tactgagaga tttccctgcg ctaccgcgca gctgtcgagg gtcacttcgc gattcatcac
    31921 caccgaaccg gtgcgcgagt taatcactac tttagcgtcc tgcggggtga cattaacctg
    31981 catattctgg atatcggcaa ggaaacggac ctgggaactg ttgccacttg gtacgcgcac
    32041 ctgaatagtc cgcgcatcta acgcggtggc gctgccatat ccacgcacgc ggttgatggt
    32101 gtcagcgatt tgctgcgcca tgctgaaatc ttcgtcgtta agttgcaaat taagggtatt
    32161 cccgacgcca aactggctgg gcaattcacg ttcaataacc gcaccattgg tgatccgtcc
    32221 accgttcagt tggttaacct gaacactgct accgcctgcg gaggctcctg cgccgccaac
    32281 cagaatattg ccctgcgcca gcgcatacac ctgactgtca acgcccttaa gcggagtcat
    32341 caacaacgta cctccacgca agcttttggc attacccatg gaagaaacca ccacatcgat
    32401 ggtttgcccc tgacgtccaa acggaggaag tgacgctgtc accattaccg cagcgacgtt
    32461 ttttagctgc atattggtgc ccgtcggaac ggtaattccc agctgtgaga gcatgttatt
    32521 aagcgtttgt gtggtaaacg gcgtctgggt tgtctggtca ccagtgccat ccagccccac
    32581 taccaagcca tagccaatca gtgagttttg ccttaccccc tgaacactgg tgagatcgcg
    32641 aatacgctca gcctgagccg ccgtcgtaac cagtagaaga attaatgcag agagaaattt
    32701 aatcaccaca gcctcactta cattggcgac aggttaagga agaaacgctg caaccagccc
    32761 atattttgcg cttcgttaat gtagccattg cctacgtatt caatacgcgc atccgccacc
    32821 tgagtagacg gtacggtatt gctgccgctg atagtgcgtg gattaaccac gcccgagaag
    32881 cgaataaatt cggtaccctg attaatggca atctgttttt cacccaccac atgcaggttg
    32941 ccgttgacca gtacctggtc aaccgtcacc gtcaacgtgc cgctaaaggt attgctggca
    33001 ttggccccgc cctttccgtt gaacgtgtta ccaccggagg cttcgacatc ggcacgagcg
    33061 ttaccaaaca gcccctgcaa atagcgcggc acagtatcaa agccaaaatt agttttaccg
    33121 tcacggctgg cattcgcaga ggagcttttg ctggcgctga cgttctcctg caacacgatg
    33181 gtcagcgtat cgccaatatt gcgtggtcga cgatcttcaa acagcggttg atagccatag
    33241 ttaatcggct gagcagactg gaaaatagaa ccgttggcga cgggcgtcgg accgggaacc
    33301 ggttgtgcac tggtcgcccc ctgcaccagc ggcgtggagg gtatccaggc gcagccggtt
    33361 agtgaaagca ccaacaagct ggaaatggca taagtatgcg cagcgttttt ttgcatggct
    33421 atcatcttca aaaatcagta aaccggtgaa cctgccaccg gttatgcctt agagttgcgt
    33481 cagtttttgc agcatctgat cggtggtgga caccgcttta ctgttgattt cgtaagcgcg
    33541 ttgcacctga atcatattga ccagttcttc cgccacgttg acgttagacg tttcaacata
    33601 cccttgatac agcagtcccg cgccgttcag gcccggcgtg ctttcgttcg gtgcaccgga
    33661 ggattgcgtt tcggtgtaga ggttttcgcc aatgctctcc agtccggtat cattcataaa
    33721 ggtggtgaga ttgagctgcc caacctgaac cggagctgcc tggccttgtt gggttacgct
    33781 gaccacgcca tcacgaccga tggtgatact taacgcattc gccggaatgg tgatcgctgg
    33841 ctgcacctga aaaccaccag ccgtcaccag ctgcccgttc tgatccacct ggaaagagcc
    33901 gtcacggata taggctgatg agccatctgg caacattacc tggaaaaagc cctgcccttt
    33961 aatcgcgaca tctttgctgt tattggtctg cgacagattg ccctggctgt gtaagcgttc
    34021 agtggcgacc gggcgtaccc ccgtgccgat ttgtaatccg gagggtaagg tggtttgttc
    34081 ggaagactgt gcccccggct ggcgaatggt ttgataaagc agatcttcaa acaccgcgcg
    34141 ctgacgctta aaaccgttag tactgacgtt tgccaggttg ttggcaatga cgtccatatt
    34201 ggtttgctgg gcgtcaaggc ccgttttggc gatccataat gaactgatca tataatgtcc
    34261 tgtagtggat tgctgacaag cagagcagaa gcacgcgaca gtgtgaattt cccctcaccc
    34321 taaccctctc cccaaagggg cgagggaata gtccgcgcgc ttttattgca atgtcatcaa
    34381 tcgttcaatt aactcatcga cagcagttgg ttggcacggc ctgcgttatc atcgacgctg
    34441 ctgatcacct tcatctgcat ttcaaaacgc cgcgcgctgg caatcatgtc gctcattgcc
    34501 gcaacggcat tgacgttact gccttccaga acccccgaca tcacacgcaa ggttggatct
    34561 gcctgcagta ccggccctcg cgtggcctgg gtttctgcgc ttaaacgaaa aatgccgtcg
    34621 tcaccgcgct gcacttcgct gcccgtggct ttcaccagtt tcagacgccc tactggcgca
    34681 actgtatttg ccggatcgcc cggattgagc gccgagattg tgccatcggc agcaatcgtg
    34741 atttccgccc cttccggcac agcaattggc cccgcctcgc ctatcaccgg atgcccctga
    34801 attgtcagtt gcccggtggg atcaacctga atgctgccat tacgcgtata cccttcgctg
    34861 ccgtcagcgg tctgcacggc cagccatcct gctgcaacgc aacgtccagc gggcgagagg
    34921 tgtaatccat cttgccgggc gtcatgtctg cacccggtgt tgacgccgtt accagcgtgc
    34981 gcgtgggcag tgaaagccct tccaccggca ctgcgcgcaa ggcgtttaac tgcgcgcgaa
    35041 aaccgggcgt agaggcattg gccagattac tggcggttac cgcctgttga ttcagcgtct
    35101 ggctggctgc tcccatcgca gtataaattg cgtgatccat tgagctatcc cgtcagcgat
    35161 tagcgtaagt taacccgcgt gttgaggatc tggtcctggg ttttgatggt ctgggcgtta
    35221 gacttatagt tacgctgggc aacgatcata ttgaccagtt ctttactgag atcgacgttg
    35281 gacgcttcca gcgcaccgtt ggtcagagtg ccaaagtttc ccgtcccggc tgtccccaac
    35341 agcgccacgc cagaagattg cgtcgcagac cagacgttgt cgccttcgga tgccagacct
    35401 tcgttgttgg caaagttcgc cagtacaatc tgccccagca gttgggtttg ttcgttggaa
    35461 ttgttgccga caaccgtacc gtcatcattg atttgataac tcaccagatc gcccggtttg
    35521 tagccattct gggtggttgc cacaatgttg ttagcgccgg ttgcgatatt attcgccatc
    35581 gcaccatcca ctaatgtgcc attagcatta aattccagtg ttgtcgctgt cttcgcaatg
    35641 ctgtttggat cactgctatc ctgggtgtag acctgccagt tattatcccc ggtcttcaca
    35701 aaatagacgc tcatgtcatg agcattaccc tgactgtcga aaacagtcac cgaacctttt
    35761 ttgttatagc tatccgcatt gctggcgctg aatggcgtaa cagtaggaag cggatcactg
    35821 gaattcaggt tgatctgcat cgacgccgtg gtggtagttt tcgctgccat cagggtattc
    35881 gggatcgaaa tattggtcgg attcgcccct tgctgaatag tcggcggcgt accggttacc
    35941 gggtaacccg tcagctgtaa accttgcgta ttcaacaggt tacggttttc atccagctta
    36001 aattgtccgt tacggctgta gaacaccgaa ccgttgctgt ctaccagacg gaaaaaaccg
    36061 ttctggctga tagcaacgtc cagacctcgc ccggtgttgg tggtcgtgcc atcggtaaag
    36121 tcctgagtga taccggcaac ttttaccccc agtcccactt tcgaaccggc aaacatatcg
    36181 gcaaaagagg ccgtgcctga tttaaagccg taggtggcgg agttggcgat attgttgcca
    36241 ataacatcga ggttggtggc ggcagcgttt aatccgctaa ccgcttgaga aaaggccatg
    36301 actgactcct gaaatgtgaa ggcttaaatt atctgccgta cttcgtcgag ggtggtggtg
    36361 ccgtaagtgc cgagatccag cgtattaccg ctgttgccgc ggatcacacc ctgcaccaga
    36421 gcaaactgca gcggctgggc aaccagttgt gtaccaccgt tactggcgct aatcgctaca
    36481 ttgtaagaac cgttcggcgc agtgctgcca tcagtcaacg aaccgtccca ggtgaaactg
    36541 tgaactccgg cggtcagttc accaatatca atggtgcgca caaccgcgcc atttttatcg
    36601 gtgatggtgg ccgtaacttt gtctgccgcc tgttgcagct caacaccaaa cggcgtggtc
    36661 gtggtcacag ccccttcttc actgccggtt cccgcaagaa cagttgtgcc ggggatcatc
    36721 acgccgtgac cgatcaggtt actggcctgt aacgactggc tgttatcaat ctgtccggaa
    36781 atagatccga gcgtggtatt gagtttttca atcccgctga ccgtgctgat ttgtgccaat
    36841 tgcgacgtca gctcgttgtt ttccattgga ttggtcgggt cctggttttt cagctgcgcc
    36901 accagcaaag tcagaaaact gctttgtaaa tctgcggcgt tgctgcccgt gagcgaacta
    36961 ctgctggtgg tactgacgcc ggtatttgtc ggatcggtgg tggttaccgc aatggacata
    37021 gctttctcct ttattgaccg agcgtaaggg ttttcagcat catgcttttc accgtgttga
    37081 gcacttcaac attggcctga tagctgcgtg acgccgacat ggtgttaacc atctccccga
    37141 cgacatcaac gttcggcatt tttatgtagc cctttgcatc tgccagcgga ttacctggtt
    37201 cataaaccag tttgtccggg gcctgacttt cttaacatcg gcaaccttta cgccgcctgt
    37261 cgcagcacct ggtgcagcgt taacctggaa taccacctgt tttgcccgat atggctgtcc
    37321 atcaggaccg gtcacgctat cagcatttgc cagattactg gccgccacgt tcaggcgctg
    37381 ggactgggca gttaacgccg acccggcgat atcaaaaata ttcagcagtg ccatccgtta
    37441 atttccgctc tgtaaaacgt tcatcatgcc tttgatttgc ccgctcaacg cgctaaggct
    37501 catctggtat tgcaggctgt tatcggcaaa ctgggtgcgt tcgcgatcca tatcgacggt
    37561 attaccgtca agcgaaggct ggtccggaat acggtattgc agttctgcgg taggaggcgt
    37621 cagcgcctgc gccgggatgt gctgcgttga ggtcatcgtc agtgcaacca cactggttgc
    37681 atcccgtcca cgttgcatga cttttttaag ttcactggca aaatcgatat cgcgcgcctg
    37741 ataaccaggg gtatcggcat tggcgatgtt tgctgccagc acttcctgac gctgggcgcg
    37801 cagattgagc gcctcttgtt gaaaacgtaa ggcggcgtcg agcttatcga gcatatctcc
    37861 tccgcaggta tcaaaattct gccatcagct taaatgcctt tacgcgcgcg ttatcggcgg
    37921 aataaacgca aaatgggtcg ctatttatgc cgttgatggt cattgcggac aggtacaatt
    37981 cacgttgtag aaatggctgg gggcgaaaat gctgacaata aaacgtagcg tggcgatcat
    38041 cgcgatactg ttcagtccgt taagtacggc gagcaatctc acatcgcaat tgcacaactt
    38101 ttttagcgcc caactcgcgg gggtaagtga tgaggttcgt gtttctattc gtacagcgcc
    38161 caatctacta ccgccatgcg agcagccatt gctttcgatg agcaataatt cccgcctgtg
    38221 gggcaatgtg aatgtgttgg cacgctgcgg taacgacaaa cgatatttac aggttaatgt
    38281 gcaggccaca ggaaattatg tggttgccgc gatgcctatt gtgcggggag gaaagctgga
    38341 agctggcaat gtcaaactga aacgtggacg cctggatacc ctgccaccgc gtacggtgct
    38401 ggatatcaat caacttgttg atgccgttag cctgcgcgat ctatcacccg atcaacctat
    38461 ccagttaacc cagtttcgcc aggcatggcg ggtaaaagcg ggacaacgcg tcaatgtgat
    38521 cgccagcggt gatgggttta gcgccaacgc agaaggtcag gcgctgaaca atgcagccgt
    38581 cgcacagaat gcgcgggtgc gcatggtatc ggaacaggta gtcagcggcg ttgttgatgc
    38641 agatgggaat attcttataa acctgtaagc tgttaaagat tacccgtccc ttgccgataa
    38701 ataagcaaca catgataaaa gcgccctcaa tgaggaataa accatgagta ttgatcgcac
    38761 ttcgcctctg aagcctgtaa gcaccgttca accgcgcgaa accaccgacg cgccggtaac
    38821 gaacagccgg gcggcaaaaa caaccgcctc caccagcacc agtgtgacgt taagcgacgc
    38881 gcaagcaaaa ctgatgcaac ccggcagcag tgatatcaat cttgaacgtg tcgaagcgtt
    38941 aaaactggcg attcgtaacg gtgaactaaa aatggacacc ggcaaaattg ccgatgcgct
    39001 gatcaacgaa gcgcagcaag acttgcagag taactgatcg tatgacacgt cttgcagaga
    39061 tcctcgacca gatgtccgct gtgcttaacg atctcaaaac ggtaatggat caagagcagc
    39121 aacatctctc tatggggcag atcaacggca gccagttgca atggattaca gaacaaaaaa
    39181 gctcactgct ggcgacgctg gattacctcg aacagttacg caagaaagaa cccaatacag
    39241 caaatagcgt tgatattagt caacgctggc aggaaattac tgtgaaaacg cagcaactac
    39301 gccaaatgaa tcaacataac ggctggttac tggaaggaca gattgagcgc aatcaacagg
    39361 cgctggaaat gttgaaaccg catcaggaac cgacgctata tggggcgaac ggtcagacct
    39421 caacacccca tcgcggcagt aaaaagattt cgatctgaat attatctccg gcctgcactg
    39481 caggccggaa tgcattgtta caccgtccgg cgggcaaatt ctttaacttt gaagcccagt
    39541 accgccagtg cagcgaagta cgcggcaatc cccgccagca cgaccgccat taaacgcagt
    39601 aagcgccagg gcatggtacc caatgaccac tccggcatga tatgtaacat acctaaaagc
    39661 acgccagaca tcaccagtac cgccaccacc agacgcaaca gaaacgccat ccagccgggt
    39721 tgcggagtaa agattttctg cttacgcaac tgccagtaaa gcagcgaagc attcagacac
    39781 gccgccagac caatagaaag tgacagcccg gcatgtttca acggaccaat aaacgccagg
    39841 ttcatcaatt gcgttaaaat cagcgtaacg atggcaattt tcactggcgt tttaatgtcc
    39901 tggcgggaat aaaagccagg agccaacact ttcactacaa tcaggccaat caaacccacc
    39961 gagtaggcaa ttaacgcccg ctgggtcatc agcgcatcaa acgcggtaaa tttaccgtac
    40021 tggaacagcg aaacggttaa cggacctgaa agaatgccca acgcaaccgc actcggcagt
    40081 gccaacagga aacaaagacg caacccccag tccatcaaac ggttgtattc atcatgattg
    40141 ccactggcaa aacttttcga cagcgacggc agcaaaatgg tgccaagcgc cacgcccagc
    40201 acaccggagg gaaactccat taagcggtcg gcgtaataca tccaagacac cgaaccggaa
    40261 gcaagaaacg aggcaaaaat ggtgttgatg attaaggaga tctggctcac agagacgcca
    40321 aggatcgccg gtcccatctg tttcaccacg cgcattgctc cagcatcgtg gaagttaatg
    40381 cgcggcagga ccagcatgcc gatcttcttc aggtgcggta gctgatacac cagctgcaga
    40441 atgccgccga ccgtaacagc ccacgccagc gccagcaccg gtgggttaaa gtacggtgcg
    40501 gcaaacagcg cgaaaccaat catgctgatg ttaagcagtg ttggcgcaaa cgccggaatc
    40561 gagaagcggt tccacgtatt cagaatcgct cccaccagcg acgccaggga gatcagcaag
    40621 atatagggaa aggtaatctt tagtagctgg ctggtcaggg caaatttgtc agctgtgtca
    40681 gcgaagcctg gcgcggtcac catgatcacc cacggtgcgg cgagcatgcc agcgaccgtc
    40741 acaaccgcca gcgcaagtgt cagcaggcca gaaacataag agacaaagac ccgcgtggcg
    40801 tcttcaccct gcttactttt atattccgcc agaatcggta caaatgcctg ggaaaatgcc
    40861 ccttcggcaa agatacggcg taacaagtta ggaagtttaa aagcgacgaa aaaggcgtcg
    40921 gttgccatcc ctgcgccaaa gattctggcg acaattgcgt ctcgtgcgaa gccaagcaca
    40981 cgcgaaaaca tggtcatcga gctgacggcg gccagcgatt ttaataaatt catcggtgtt
    41041 ctaatccgga cccacgggca aaacgcctgc aaggcaggcg ctgaaagagg cgaatagtct
    41101 acctagatta tggtgaattg ctaccgccag atgttacagg gtttattcac tcatcgcatc
    41161 gcgccagatc ttgtcaacga tacgttgcgc cagcactgcc tattcgccgg cggtttgcgg
    41221 aactgtctga ttttacacgc attcaatgaa gtgccgcgca cagccgacaa atccacgttg
    41281 ctcaagagtg ctctgccaac caggaatcgg tttattcact acaccctgcc cgcgctcctc
    41341 acgccattcg cgcatatccg taatgtcgat gagcgcaccg tcagtcacgg cctgcacggt
    41401 ttcacgctga cttccggcac ggcgatgcat acaagtggtg atctgcaaag gaccggccga
    41461 aaaatggtgc tcggcaaaca gcatttcgcc agcgtcgtta gtcagtagcg taccgccatc
    41521 cagagaggct ttgccgcccg acaaccacag cgcggtatcc accacatgca gataatcatc
    41581 cagcaacgtg aaataaagat cgtgtggccc gacgctattg ctacgatgtt tatccattct
    41641 tagcgaggct gcggtggcga gttgcgtttt taactcaccg tagagtggtg cgaaacgacg
    41701 gttaaaaccg accatcaacg tcagtttttt ccgcgccgcc agttccacca gcctttcagc
    41761 atcgcgcaga ttttctgcca gcggtttatc gacacagaca tgtacccccg cattgagtaa
    41821 cgtactgacc acgtcaaagt ggctggcggt gctggagtgc acaaaaaccg catcacaact
    41881 ggcggcaagg ctggataacg aatcggcata aggaatgcgc cagctgtcac aaattggcag
    41941 ggctttggcg cgcgtaggcg accaggctcc ttgtaacgtc cagtcagacg ctgccgccag
    42001 caccggtaac cacgcttttt gcgcaatgcc gcctaatccc actacgccga tacgtaattt
    42061 tttcacttta atctcccaga tgggccagca acgaatcaag acgttgtttc agttctgcca
    42121 cttcgatttc cagggcttcg acgcgggtct gtaaatcacc gtcaaccatg ttcgacatag
    42181 ccgttaccgc cggctgatct tcaacctcac cactgaaaag atgcatgtag cggctttcgc
    42241 gtttacccgg ttcgcgggcc agacgcacca caaaaggacc atcttcgcga ttcgccagtt
    42301 gttccagcgt cgactccact tccgccatat cgctgaattc atacattcgc gcggcgcggc
    42361 tgcgcagttc acccggcgtc tgggcaccac gcaataacaa cgtggtgatt aacgccactt
    42421 ctgcggcgct cagtttcaga tcgccaaatt ctgaattaca aaaacgttgc tcatatttgg
    42481 tgacccgatt accaaaaccg ctcactgtgc gtagataatg acgtttgacc agattatcca
    42541 gctgttcctg cacttcggat tcactcagat tcatgaccgg ttcacggttc gttttctgat
    42601 tacaggccgt gactacacca ttgactgaga gcgggtattg ctccggcgtg gtcacctgct
    42661 tttccagcaa gcaaccaatt acgcgagctt ccagggcagt aagttgatat ttcatgagat
    42721 tctccttagc ggccgggcgt ccagtctggg gtagttaatg ccgtcagtac gtgatcgcgc
    42781 cattgtccat caatcaacag atagtctttc gcatagcctt ctttttcaaa acccagtcgc
    42841 gccagtaaat caccgctgcg tttattgtgc ggcatataat tagccataat gcgatgaata
    42901 tgttgggtgc gctgcatata acgaatggct gcggtcaggg cttcaaacat gagtcctttg
    42961 ccctgccatt tttgcccaat cgaataaccg agatagcagg catgaaaaga gccgcgaaca
    43021 acattggaaa aattggcaac gccaataatc tctttttcgt ccgggtcgaa taagccaaag
    43081 tagaaagctg aaccttgttt atgaaattcg ttaatcatcc ctagcctggc ctgccagcct
    43141 gatggataac agtggctttc gtcgcgcact ggctcccagg gcttgaggaa atggcgattc
    43201 tctgcgtaat aatccgccag acgccaggca tcacgatcat gcaccagacg cacgaccagt
    43261 cggtctgtgg ttaagcgcac ttttggcacg ttactgcgat agccaaacat tctataccta
    43321 ctccttcccg taacgtcttt aatacgctgt aagtattact atacctgcgc aaccagaccc
    43381 tgtgaaaagt ccaaaatgcc attttctgca ttattcacat atttgataaa aactctctat
    43441 caaagcgacg ttttgataaa aaaatactgt ccccgaagtg atgggaaaag tcttagcggc
    43501 aatgcagaat agaaagtgct catcttttat ttcccgggag gggaaatgtc ccgcgtatcg
    43561 caggcgagga acctgggtaa atatttcctg ctcatcgata atatgctggt cgtgctgggg
    43621 ttctttgttg tcttcccgct gatctctatc cgcttcgttg atcaaatggg ctgggccgcc
    43681 gtcatggtcg gtattgctct cggtctacgc cagtttattc agcaaggtct gggtattttc
    43741 ggcggcgcaa ttgccgaccg ctttggtgcc aaaccgatga ttgttaccgg tatgctgatg
    43801 cgcgccgccg gattcgccac aatgggtatc gcccacgaac cgtggctatt gtggttttca
    43861 tgcctgctct cgggactcgg cggcacgttg tttgatccgc cgcgttcggc gctggtggtg
    43921 aaactaatcc gtccacaaca acgtggtcgt tttttctcgc tgttgatgat gcaggacagt
    43981 gccagtgcgg tcattggcgc attgttgggg agctggctgt tgcaatacga ctttcgcctg
    44041 gtctgcgcca caggggccgt tttgtttgtg ctatgtgcgg cgttcaatgc gtggttgtta
    44101 ccggcatgga aactctccac cgtacgcacg cccgttcgcg aaggcatgac ccgcgtgatg
    44161 cgtgacaagc gttttgtcac ctatgtcctg acgctggcgg gttactacat gctggctgta
    44221 caagtgatgc tgatgctgcc aattatggtc aacgacgtgg ctggcgcgcc ctctgccgtt
    44281 aaatggatgt atgccattga agcgtgtctg tcgttaacgt tgctctaccc tatcgcccgc
    44341 tggagtgaaa agcattttcg tctggaacac cggttgatgg ctgggctgtt gataatgtca
    44401 ttaagcatga tgccggtggg catggtcagc ggcctacaac aacttttcac cctgatttgt
    44461 ctgttttata tcgggtcgat cattgccgag cctgcgcgtg aaaccttaag tgcttcgctg
    44521 gcggacgcaa gagctcgcgg cagctatatg gggtttagcc gtctgggtct ggcgattggc
    44581 ggcgctattg gttatatcgg tggtggctgg ctgtttgacc tgggcaaatc ggcgcaccag
    44641 ccagagcttc cgtggatgat gctgggaatt attggcatct tcactttcct tgcgctgggt
    44701 tggcagttta gtcagaaacg cgccgcgcgt cgtttgctgg aacgcgacgc ctgatttgat
    44761 actcccccct gactcctcca tactgaaagt gaactgacag ccaaatggag gagtcacgtg
    44821 aagctataca tttacgatca ctgcccttac tgcctcaaag cccgcatgat tttcggcctg
    44881 aaaaatatcc ccgtcgaatt acatgttctg ctcaacgacg acgcagaaac acccacccgg
    44941 atggtcggtc aaaaacaggt tcccattctg caaaaagatg acagccgcta tatgccagaa
    45001 agcatggata tcgttcacta tgtcgataaa ctcgacggca aaccgttact gaccggcaaa
    45061 cgttcccctg ccattgaaga gtggctgcgc aaggtcaatg gctacgtcaa caaactgctg
    45121 ttgccgcgtt ttgccaaatc ggcatttgat gagttttcta ctcccgccgc gcgcaaatat
    45181 ttcgtcgaca agaaagaggc cagcgcgggt aattttgccg acctgctggc ccactctgac
    45241 ggtctgatta agaatatcag cgatgatttg cgtgcgctgg acaaactgat cgtcaaaccg
    45301 aacgccgtga atggcgaact ttcggaagat gatattcagc tatttccgct actgcgtaat
    45361 ctgacgctgg tagccggaat caactggcca agccgcgttg ctaattaccg cgataatatg
    45421 gcgaaacaga cacaaatcaa tttgttatca tcaatggcga tttaatcctg acccgccgcg
    45481 gcagcctgta ttcgcgggtt gccgccgttt tcagtttttt ctgctggcga gtttatcgct
    45541 ggtgacacat gctgcgttgt gtcaggatat tgacgcagat ttcatgtcga ggactaccat
    45601 gaacaagttt ttatttgccg ctgcattgat cgtcagtggc ctgctcgttg gctgtaatca
    45661 actcacccaa tacaccatca ccgaacaaga aattaaccag tcgcttgcga aacataataa
    45721 tttctcaaaa gatatcggtt tacccggcgt ggctgacgcc catattgttc tgacaaactt
    45781 gaccagccaa attggtcgcg aagagccgaa taaggttacc ctaaccggag acgccaatct
    45841 ggacatgaac tccctgttcg gtagtcagaa agcgaccatg aaactgaagc tgaaagcgct
    45901 gcccgtgttt gataaagaaa aaggtgcgat cttcctgaaa gagatggaag tggtcgatgc
    45961 gacggtacaa ccggaaaaaa tgcaaacggt gatgcaaacg ttgcttccct atttgaacca
    46021 ggcattacgc aattacttta accagcaacc tgcttacgtc ctgcgcgaag atggcagtca
    46081 gggcgaagca atggcgaaaa aactggcgaa aggcattgaa gtgaagccag gcgaaattgt
    46141 cattccgttt acggattaat gacgagggcg cattcgcgcc ctttattttt cgtgcaaagg
    46201 aaaacgtttc cgcttatcct ttgtgtccgg caaaaacatc ccttcagccg gagcatagag
    46261 attaatgact gcaccatccc aggtattaaa gatccgccgc ccagacgact ggcaccttca
    46321 cctccgcgat ggcgacatgt taaaaactgt cgtgccgtat accagcgaaa tttatggacg
    46381 ggctatcgta atgcccaatc tggctccgcc cgtgaccaca gttgaggctg ccgtggcgta
    46441 tcgccaacgt attcttgacg ccgtacctgc cgggcacgat ttcaccccat tgatgacctg
    46501 ttatttaaca gattcgctgg atcctaatga gctggagcgc ggatttaatg aagccgtgtt
    46561 caccgctgca aaactttacc cggcaaacgc aaccactaac tccagccacg gcgtgacgtc
    46621 aattgacgca atcatgccgg tacttgagcg catggaaaaa atcggtatgc cgctactggt
    46681 gcatggtgaa gtgacacatg cagatatcga catttttgat cgtgaagcgc gctttataga
    46741 aagcgtgatg gaacctctgc gccagcgcct gactgcgctg aaagtcgttt ttgagcacat
    46801 caccaccaaa gatgctgccg actatgtccg tgacggaaat gaacggctgg ctgccaccat
    46861 cactccgcag catctgatgt ttaaccgcaa ccatatgctg gttggtggcg tgcgtccgca
    46921 cctgtattgt ttacccatcc tcaaacgcaa tattcaccaa caggcattgc gtgaactggt
    46981 cgccagcggt tttaatcgag tattcctcgg tacggattct gcgccacatg cacgtcatcg
    47041 caaagagagc agttgcggct gcgcgggctg cttcaacgcc ccaaccgcgc tgggcagtta
    47101 cgctaccgtc tttgaagaga tgaatgctct gcagcacttt gaagcattct gttctgtaaa
    47161 cggcccgcag ttctatggct tgccggtcaa cgacacattc atcgaactgg tacgtgaaga
    47221 gcaacaggtt gctgaaagca tcgcactgac tgatgacacc ctggtgccat tcctcgccgg
    47281 ggaaacggta cgctggtccg ttaaacaata aaaatcgtag cttcctgttg tcattaggtt
    47341 attttacctg tataaataac cagtatattc aacagggggc tattatgcga attgaagtca
    47401 ccatagcgaa aacttctcca ttgccagctg gggctattga cgccctggct ggcgaacttt
    47461 cccgccgtat tcagtatgcg tttcctgata atgaaggcca cgtatcggta cgttatgccg
    47521 cagcgaataa tttatcggtt attggcgcaa caaaagaaga taaacagcgc attagcgaaa
    47581 ttctccagga aacgtgggaa agcgccgatg actggtttgt cagcgaataa tatgcagtga
    47641 tttttgtttt tgccggatcg ccccggcttt tttattttac ttacgaattt tgagtacaaa
    47701 atttctacac ttcctcaaaa taattgcaca atatgctaaa aaaatatgaa tgacatgctg
    47761 ttttattgtt catataaatc ttaaaagcag aaatttatct gttcccccct ttaaaaattc
    47821 ggtgaatacc cttacttatt ggtatactga agttgcttca gataaaaacg cattgaacct
    47881 cgaataacgt tgtctagtaa cacgaattag ggggccatga tggaaaaaaa taatgaagtc
    47941 attcagactc atccgctcgt agggtgggac atcagcaccg ttgatagcta tgatgcgctg
    48001 atgttgcgtt tgcactacca gaccccaaat aagtccgagc aggaagggac tgaagttggt
    48061 cagacgctct ggttaaccac tgatgttgcc agacaattta tttcgatatt agaagcagga
    48121 atcgccaaaa ttgaatccgg tgatttcccg gtaaacgagt atcggcgtca ttaacatgac
    48181 atgaccatcc acccaaaagg cacctcaccg gtgcctttac catttctggg ttgtatatct
    48241 gcattcagtt aattactctg cttaaaccat gagagcagag agaaaaaaat gaaatacgat
    48301 ctcatcatta ttggcagcgg ttccgtaggc gctgccgccg ggtattatgc aacccgcgcc
    48361 ggtttaaacg tgctaatgac cgacgcccat atgccaccgc atcaacacgg cagccaccac
    48421 ggcgatacgc gattaattcg ccatgcttat ggtgaaggcg aaaagtatgt cccgttggtc
    48481 ctccgcgcgc aaacgctgtg ggatgaactt tcccgccaca acgaagatga tcccattttt
    48541 gtacgctctg gtgtcattaa cctcggcccg gctgactccg catttctcgc caacgtcgcc
    48601 cacagcgccg aacagtggca actcaacgtt gaaaagctcg atgcgcaagg gattatggcc
    48661 cgctggccag aaatacgcgt cccggacaac tacatcggct tatttgagac tgattccggt
    48721 tttttgcgca gcgaactggc gattaaaacc tggatccaac tggcgaagga agcgggctgt
    48781 gcgcaactgt tcaactgccc ggtcaccgca attcgtcatg acgatgatgg cgtaactatt
    48841 gaaacggttg acggtgagta tcaggcgaaa aaagcgattg tctgcgcggg aacatgggta
    48901 aaagacctgc tcccggagct gcctgtccag cccgtacgta aagtatttgc ctggtatcag
    48961 gccgatggcc gctatagcgt gaagaataaa ttcccggcgt ttaccggtga actgcccaat
    49021 ggcgatcaat attatggttt tccggcagaa aacgacgcgt tgaagattgg caaacataac
    49081 ggaggccagg ttatccattc agcggatgaa cgtgttccgt ttgcggaagt ggccagtgat
    49141 gggtcagaag ccttcccgtt cttgcgcaat gtattgccgg gtatcggttg ctgcctgtac
    49201 ggcgctgcct gcacctatga taattcgcct gacgaagatt ttattatcga taccctaccc
    49261 ggccacgata atacactgct cattaccggc ctgagtgggc acggttttaa atttgcgtca
    49321 gttttagggg aaatagctgc cgattttgcg caagaccaaa aaagcgattt tgatttgacg
    49381 ccattcaggc tttcccgctt ccaataatca tcattacggc ctccagaatg aggccgtttt
    49441 tacaggagta tttaatgcgt cggctgttgc actatctcat caataatatt cgcgagcatc
    49501 tgatgcttta tctttttctc tggggattgt tggcaattat ggatctgatc tatgtgtttt
    49561 atttttagtt ttccgcactg acataaactg acaaatattt aatctccctt tcatttagcg
    49621 tgaaaaattg aatgagatta ttcctgcttc tacagcactc accagacatc tctgatgctg
    49681 aacccactaa acttatgaag attctttcat tattaattca aaattattga aataaaaata
    49741 aatatcattc aatttgttat ttttgtgttg caattcattc tccgacacgc cattttcttt
    49801 ctctcgcaaa ttcgtattgg atgcacttat gtcattcaca aatacccctg aacgctatgg
    49861 tgttatatct gcggccttcc attggctaag cgcaattatt gtctacggca tgtttgcttt
    49921 aggtctgtgg atggtcacgc tcagttatta cgatggctgg tatcacaaag cacccgaact
    49981 gcataaaagc atcggtattt tgttaatgat ggggctggtt attcgcgttc tgtggcgtgt
    50041 catatctcca ccgccgggtc cgctgccgag ttattcgccc atgactcgtc ttgccgcaaa
    50101 agctggacat ctcgcattgt gcctattgct gttcgccatc ggcatcagcg gctatctgat
    50161 ctcaactgcc gatggtaaac cgatcagcgt ttttggctgg tttgacgtcc ccgcgaccct
    50221 tgccgacgct ggcgcacagg cagactttgc cggtgccctg catttttggc tcgcgtggag
    50281 cgtcgtagta ctgtctgtta tgcacggatt tatggccctg aagcatcatt tcatcgataa
    50341 agacgacact ctgaagcgca tgctgggaaa gtcgtcatct gactatggag tataaaaaat
    50401 gaaaaaaagc ctgcttggtt taaccttcgc gtccctgatg ttctctgccg gttcagcggt
    50461 tgccgccgat tacaaaattg acaaagaagg tcagcacgcc tttgttaact tccgcataca
    50521 gcatcttggc tatagctggt tatatggcac ctttaaagat ttcgacggta cttttacctt
    50581 tgatgaaaaa aatccggctg ccgataaagt gaatgtgaca attaacacca ccagcgtcga
    50641 tactaaccac gccgaacgcg ataaacatct tcgcagtgcg gattttctca ataccacaaa
    50701 atatccacag gcaacattca cctccaccag cgtgaagaaa gacggtgacg aactggatat
    50761 taccggcgat ctgacgctga atggcgtaac caaacccgtc acgctggaag cgaaattaat
    50821 tggtcagggc gacgacccat ggggcggtaa acgtgcaggc tttgaggccg aaggcaaaat
    50881 taagctcaaa gacttcaata tcaagacaga tttaggtcca gcttctcagg aagtggatct
    50941 gattatttca gtggaaggcg tacagcagaa gtaagagtga aatgttgccg gatggcacgc
    51001 atccggcaat gatatttatt ctgttggatc aggaataccc agtgttgtat tcagacgtcc
    51061 acgagactta ttaaagatct tattgccatt ttcacgtcct gcccgacggc gtcgctgttc
    51121 ttctggcggt aacgcgcttt cttcgcagca aatctcacta caacaacctt tgtatttttc
    51181 cgcgcatact ggacactgaa taaacagcag gtggcagcca tcatttttac agttggtatg
    51241 gctgtcgcac ggcgcgccgc actggtggca atgcgcgata atctcatccg atatacgttc
    51301 gcccatccgc tcgtcaaaaa caaaattttt gccaataaaa cgcaccggca agccctgctc
    51361 gcgcgcctta cgggcgtatt caataattcc gccctcgata tgccagactt tgttgaatcc
    51421 gttatgtttc atccaggcac tggccttttc acaacgaatg ccgccggtgc agtacatgac
    51481 gattttttta tctttatgtg cctgcatcat ctcgactgct tttggcagct gctcacggaa
    51541 ggtatctgcc ggaatttcga gcgcgttttc aaagtgcccc acttcatact catagtggtt
    51601 acgcatgtcg ataaatagtg catcgggatc gtcaagcatg gcgttcactt ccgccgcttg
    51661 cagatactca ccaacattgc tggcatcaaa gtgaggatcg tcaataccgt cggcaacaat
    51721 gcgatcgcgt accttcatgc gcagtaccca gaaggatttc ccgtcatcat ccaacgcgat
    51781 attcaggcgt aaaccctcta aagccgggtc gaaggcatag agctgcgcgc gaaatgtttc
    51841 aacattgctc gcaggtacgc tgatttgcgc gttaatgccc tcatgcgcca gatacactcg
    51901 cccaaaaaca ttcagcgcgg taaacagctg atataaagcg tcacgggtcg ccttaggatc
    51961 ggcgatgtgg aaatacttgt aaaacgaaat ggtggttcgc ggttcgctct cagccaacat
    52021 tttggctttt aacgcgtcgt tggaaatgcg gttgtgtaac actggcatgg tgtacggttc
    52081 ctgcgagatg ggaaagtaaa aatccgcggc atgatatagc aattatcgat aattaacatc
    52141 cacacatttt acgctacatt tgcgcattaa aaattatttg ttatttacaa gcgcggcaat
    52201 ttcgcccagt cttcagccac aaattttggt tgcgggcgaa aaaatgcgac aatacataca
    52261 attgcccgta taggttgaaa aacaggattg atatgacgaa tctacccaag ttctccaccg
    52321 cactgcttca tccgcgttat tggttaacct ggttgggtat tggcgtactt tggttggtcg
    52381 tgcaattgcc ctacccgatt atctaccgcc tcggttgtgg attaggaaaa ctggcgttac
    52441 gttttatgaa acgacgcgca aaaattgtgc atcgcaacct ggaactgtgc ttcccggaaa
    52501 tgagcgaaca agaacgccgt aaaatggtgg tgaagaattt cgaatccgtt ggcatgggcc
    52561 tgatggaaac cggtatggcg tggttctggc cggaccgccg aatcgcccgc tggacggaag
    52621 tgatcggcat ggaacacatt cgtgacgtgc aggcgcaaaa acgcggcatc ctgttagttg
    52681 gcatccattt tctgacactg gagctgggcg cgcggcagtt tggtatgcag gaaccgggga
    52741 ttggcgttta ccgcccgaac gataatccac tgattgactg gctacaaacc tggggccgtt
    52801 tgcgctcaaa taaatcgatg ctcgaccgca aagatttaaa aggcatgatt aaagccctga
    52861 aaaaaggcga agtggtctgg tacgcaccag atcatgatta cggcccgcgc tcaagcgttt
    52921 tcgtcccgtt atttgccgtt gagcaggctg cgaccacgac cggaacctgg atgctggcac
    52981 ggatgtccgg cgcatgtctg gtgcccttcg ttccacgccg taagccagat ggcaaagggt
    53041 atcaattgat tatgctgcca ccagagtgtt ctccgccact ggatgatgcc gaaactaccg
    53101 ccgcgtggat gaacaaagtg gtcgaaaaat gcatcatgat ggcaccagag cagtatatgt
    53161 ggttacaccg tcgctttaaa acacgcccgg aaggcgttcc ttcacgctat taaatctccc
    53221 atgccggatg cttcagaatg gcatccggca ttaccacagc aaatccccct gatttagcga
    53281 taaaagctct ctggattgcg ccccctggaa gtcgggcgca taattagtgt gtttatcttt
    53341 tcttcttatg ttcaccgcgc ctggcgcacc aacagcggat tgctatgtca ccctgtgaaa
    53401 atgacacccc tataaactgg aaacgaaacc tgatcgtcgc ctggctaggc tgttttctta
    53461 ccggggccgc cttcagtctg gtaatgccct tcttacccct ctacgttgag cagcttggtg
    53521 ttaccggcca ctccgccctg aatatgtggt ccggtattgt cttcagtata atgggttcct
    53581 aaacacatca ttttaggtat tgaatatgag acgggcaatc agctacatca gattttcaag
    53641 tgaacgacaa ctgaaagggg actctgtacg ccgacaatct aagctggtta ctgactggct
    53701 tgataagaac cctgagtttt acttggattc atccttgagc tttaaagatt tggggaaaag
    53761 tgcttttagt ggtaagcacc tgaaaggtgg tctaggtgac tttttaaccg ctatcgagaa
    53821 agggcttgtt aaagctggtg atacgctact aatcgagagt ttagaccgtc tgtcacgtca
    53881 ggatattgat attgcttctg agctattacg ccgtatctta agggctggtg tagatgtagt
    53941 cacactgagc gatggagaac attacacacg agaaagttta aaagacccgt tagccctgat
    54001 taaaagcatt ctgataatgc agcgggcgca tgaggaaagt ttgagaaagt ctgagcgtgt
    54061 ccaagctgca tggaaccgta aaaaagaact catctccgaa ggtatcaagg tatctaggcg
    54121 ttgtcctgca tggttaaggc tgaatgatga ccgtcgaaca ttcacaatca ttcctgataa
    54181 agtggaagtt gttaaacgag catttgacct gagattacag ggtttaagct tttgggctat
    54241 caccagaact ttaaacgatg aaggtcactt gtcattgaat caatacacac ccaaacaaaa
    54301 ggggtggtct gatactgctg taaagaaact gctcagaaat cgtgctgtga ttggatgttt
    54361 cacacctgct ggacgtgaag aagttcaggg ttactacccc gcaataatca gtgagtcact
    54421 attctatcga gtacagcaac tgaacacagg gcaatatggt agggcttctg tatcaagtaa
    54481 tccgctatca gtcaacttgt tcaggggtat catcaa
//
LOCUS       DAZSIO010000006        54391 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZSIO010000006 DAZSIO010000000
VERSION     DAZSIO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.006"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            533..1822
                     /gene="sdaC"
                     /locus_tag="ACHX5T_000310"
     CDS             533..1822
                     /gene="sdaC"
                     /locus_tag="ACHX5T_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR8465657.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1880..3247
                     /gene="sdaB"
                     /locus_tag="ACHX5T_000311"
     CDS             1880..3247
                     /gene="sdaB"
                     /locus_tag="ACHX5T_000311"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR8465658.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3358..4113
                     /gene="xni"
                     /locus_tag="ACHX5T_000312"
     CDS             3358..4113
                     /gene="xni"
                     /locus_tag="ACHX5T_000312"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR8465659.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="ACHX5T_000313"
     CDS             complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="ACHX5T_000313"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR8465660.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="ACHX5T_000314"
     CDS             complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="ACHX5T_000314"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR8465661.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6536..7852
                     /gene="fucP"
                     /locus_tag="ACHX5T_000315"
     CDS             6536..7852
                     /gene="fucP"
                     /locus_tag="ACHX5T_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR8465662.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAATPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7885..9660
                     /gene="fucI"
                     /locus_tag="ACHX5T_000316"
     CDS             7885..9660
                     /gene="fucI"
                     /locus_tag="ACHX5T_000316"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFR8465663.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9769..11188
                     /gene="fucK"
                     /locus_tag="ACHX5T_000317"
                     /pseudo
     CDS             9769..11188
                     /gene="fucK"
                     /locus_tag="ACHX5T_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11190..11612
                     /gene="fucU"
                     /locus_tag="ACHX5T_000318"
     CDS             11190..11612
                     /gene="fucU"
                     /locus_tag="ACHX5T_000318"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR8465664.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11670..12401
                     /gene="fucR"
                     /locus_tag="ACHX5T_000319"
     CDS             11670..12401
                     /gene="fucR"
                     /locus_tag="ACHX5T_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR8465665.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="ACHX5T_000320"
     CDS             complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="ACHX5T_000320"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR8465666.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13538..13933)
                     /locus_tag="ACHX5T_000321"
     CDS             complement(13538..13933)
                     /locus_tag="ACHX5T_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR8465667.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="ACHX5T_000322"
     CDS             complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="ACHX5T_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR8465668.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15220..15447)
                     /locus_tag="ACHX5T_000323"
     CDS             complement(15220..15447)
                     /locus_tag="ACHX5T_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR8465669.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15639..16844
                     /gene="csdA"
                     /locus_tag="ACHX5T_000324"
     CDS             15639..16844
                     /gene="csdA"
                     /locus_tag="ACHX5T_000324"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR8465670.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16844..17287
                     /gene="csdE"
                     /locus_tag="ACHX5T_000325"
     CDS             16844..17287
                     /gene="csdE"
                     /locus_tag="ACHX5T_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR8465671.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="ACHX5T_000326"
     CDS             complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="ACHX5T_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR8465672.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18382..19479)
                     /gene="mltA"
                     /locus_tag="ACHX5T_000327"
     CDS             complement(18382..19479)
                     /gene="mltA"
                     /locus_tag="ACHX5T_000327"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR8465673.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19688..19764
                     /locus_tag="ACHX5T_000328"
     tRNA            19688..19764
                     /locus_tag="ACHX5T_000328"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19722..19724,aa:Met,seq:cat)
     gene            complement(19839..21092)
                     /gene="amiC"
                     /locus_tag="ACHX5T_000329"
     CDS             complement(19839..21092)
                     /gene="amiC"
                     /locus_tag="ACHX5T_000329"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR8465674.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21324..22655
                     /gene="argA"
                     /locus_tag="ACHX5T_000330"
     CDS             21324..22655
                     /gene="argA"
                     /locus_tag="ACHX5T_000330"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR8465675.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22717..24543)
                     /gene="recD"
                     /locus_tag="ACHX5T_000331"
     CDS             complement(22717..24543)
                     /gene="recD"
                     /locus_tag="ACHX5T_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR8465676.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24543..28085)
                     /gene="recB"
                     /locus_tag="ACHX5T_000332"
     CDS             complement(24543..28085)
                     /gene="recB"
                     /locus_tag="ACHX5T_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR8465677.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28078..30966)
                     /gene="ptrA"
                     /locus_tag="ACHX5T_000333"
     CDS             complement(28078..30966)
                     /gene="ptrA"
                     /locus_tag="ACHX5T_000333"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFR8465678.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31142..34510)
                     /gene="recC"
                     /locus_tag="ACHX5T_000334"
     CDS             complement(31142..34510)
                     /gene="recC"
                     /locus_tag="ACHX5T_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR8465679.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34523..34873)
                     /locus_tag="ACHX5T_000335"
     CDS             complement(34523..34873)
                     /locus_tag="ACHX5T_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR8465680.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(34858..35265)
                     /locus_tag="ACHX5T_000336"
     CDS             complement(34858..35265)
                     /locus_tag="ACHX5T_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR8465681.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35262..35825)
                     /locus_tag="ACHX5T_000337"
     CDS             complement(35262..35825)
                     /locus_tag="ACHX5T_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR8465682.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35816..36286)
                     /locus_tag="ACHX5T_000338"
                     /pseudo
     CDS             complement(35816..36286)
                     /locus_tag="ACHX5T_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36470..37264)
                     /gene="thyA"
                     /locus_tag="ACHX5T_000339"
     CDS             complement(36470..37264)
                     /gene="thyA"
                     /locus_tag="ACHX5T_000339"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR8465683.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37271..38146)
                     /gene="lgt"
                     /locus_tag="ACHX5T_000340"
     CDS             complement(37271..38146)
                     /gene="lgt"
                     /locus_tag="ACHX5T_000340"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR8465684.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38297..40543)
                     /gene="ptsP"
                     /locus_tag="ACHX5T_000341"
     CDS             complement(38297..40543)
                     /gene="ptsP"
                     /locus_tag="ACHX5T_000341"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR8465685.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40556..41086)
                     /gene="rppH"
                     /locus_tag="ACHX5T_000342"
     CDS             complement(40556..41086)
                     /gene="rppH"
                     /locus_tag="ACHX5T_000342"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR8465686.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(41439..41585)
                     /gene="ygdT"
                     /locus_tag="ACHX5T_000343"
     CDS             complement(41439..41585)
                     /gene="ygdT"
                     /locus_tag="ACHX5T_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR8465687.1"
                     /translation="MLSTESWDNCEKPPLLFSFTALTCDETPVFSGSVLNLVAHSVDK
                     HGIG"
     gene            41771..42460
                     /gene="mutH"
                     /locus_tag="ACHX5T_000344"
     CDS             41771..42460
                     /gene="mutH"
                     /locus_tag="ACHX5T_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR8465688.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42529..43242
                     /locus_tag="ACHX5T_000345"
     CDS             42529..43242
                     /locus_tag="ACHX5T_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR8465689.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43380..43598
                     /gene="ygdR"
                     /locus_tag="ACHX5T_000346"
     CDS             43380..43598
                     /gene="ygdR"
                     /locus_tag="ACHX5T_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR8465690.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43706..44746
                     /locus_tag="ACHX5T_000347"
     CDS             43706..44746
                     /locus_tag="ACHX5T_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR8465691.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44779..45969)
                     /gene="lplT"
                     /locus_tag="ACHX5T_000348"
     CDS             complement(44779..45969)
                     /gene="lplT"
                     /locus_tag="ACHX5T_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR8465692.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(45962..48121)
                     /gene="aas"
                     /locus_tag="ACHX5T_000349"
     CDS             complement(45962..48121)
                     /gene="aas"
                     /locus_tag="ACHX5T_000349"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR8465693.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48707..49738
                     /gene="galR"
                     /locus_tag="ACHX5T_000350"
     CDS             48707..49738
                     /gene="galR"
                     /locus_tag="ACHX5T_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR8465694.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49745..51007)
                     /gene="lysA"
                     /locus_tag="ACHX5T_000351"
     CDS             complement(49745..51007)
                     /gene="lysA"
                     /locus_tag="ACHX5T_000351"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR8465695.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51129..52064
                     /gene="lysR"
                     /locus_tag="ACHX5T_000352"
     CDS             51129..52064
                     /gene="lysR"
                     /locus_tag="ACHX5T_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR8465696.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52051..52743)
                     /gene="ygeA"
                     /locus_tag="ACHX5T_000353"
     CDS             complement(52051..52743)
                     /gene="ygeA"
                     /locus_tag="ACHX5T_000353"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFR8465697.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52872..54290)
                     /gene="araE"
                     /locus_tag="ACHX5T_000354"
     CDS             complement(52872..54290)
                     /gene="araE"
                     /locus_tag="ACHX5T_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR8465698.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT        13292 a        14727 c        13414 g        12958 t
ORIGIN      
        1 ttgccgggtt ttctcgtttt tggtcactta ctcatcaact catttcattt gttatatgaa
       61 tgtttcttac caccctcacg cgacaaatat catcacagtt aatatgtcat acaatttatg
      121 ttgcaacaca aacgtttccc tattttcata aaccgtattt tatcgctatg aaaaagaaat
      181 tatcgccatg attaactaaa agtattgatt ttttcagttc aacctacata tattgcgcgc
      241 cccggaagaa gtcagatgtc gtttaatggg caaatattgc ccttaaattc tcttttactt
      301 ttgatttaca gagtaaagcg ttgggataat ctatcttcca agcagattat tgtatttggg
      361 atcaagatca ctgatagata cataacttgt gtgtatcttt ccgccctcaa attattacgg
      421 cggtaaatga ttaagccatc gccgatagac agatttcatt tttacggtca ggcaccttcc
      481 cgggctgaac tggctaaaag ctgaattatt tgcattcctc caggagaaat agatggaaac
      541 gactcaaacc agcacgattg cgtcgaaaga ctctcgtagt gcctggcgca agacagacac
      601 catgtggatg ctgggccttt acggcacggc aatcggcgcg ggcgtgctgt tcctgccaat
      661 caacgccggt gttggcggta tgatcccgct gatcatcatg gctatccttg cgttcccgat
      721 gacgtttttt gctcaccgcg gcctgactcg cttcgtactg tctggtaaaa acccgggcga
      781 agacatcacc gaggttgtag aagaacactt tggtattggc gcaggtaaac tgattaccct
      841 gctctacttc ttcgctatct acccgatcct gctggtttat agcgtggcaa tcaccaatac
      901 cgttgaaagc ttcatgtctc accagctggg tatgacgcca ccgccgcgtg cgattctgtc
      961 gctgatcctg atcgtgggta tgatgaccat cgttcgcttt ggtgagcaga tgatcgttaa
     1021 agcgatgagt attctggtat tcccgtttgt tggcgtactg atgctgctgg ctctgtacct
     1081 gatcccgcag tggaacggcg cagcactgga aacgctgtct ctggacactg catctgcaac
     1141 cggaaacggt ctgtggatga ccctgtggct ggcaattccg gtaatggtgt tctcgttcaa
     1201 ccactctccg atcatctctt ctttcgccgt tgcgaagcgt gaagagtacg gcgatatggc
     1261 agaacagaaa tgctcgaaga tcctggcatt cgcacacatc atgatggtgc tgaccgtaat
     1321 gttcttcgtc ttcagctgcg tactgagcct gactccggca gacctggctg cggctaaaga
     1381 gcagaacatc tcgattctgt cttacctggc taaccacttt aacgcaccgg ttatcgcgtg
     1441 gatggctccg attatcgcga ttatcgctat caccaaatcc ttcctcggcc actacctggg
     1501 cgcacgtgaa ggcttcaacg gtatggtgat taaatctctg cgtggtaaag gtaagtctat
     1561 cgaaatcaac aagctgaacc gtatcactgc gctgttcatg ctggtaacga cctggattgt
     1621 tgccaccctg aacccgagca tcctgggtat gattgaaacc ctgggcggcc caatcatcgc
     1681 gatgatcctg ttcctgatgc cgatgtacgc aattcagaaa gtaccggcaa tgcgtaagta
     1741 cagcggtcac atcagcaacg tattcgttgt cgtgatgggt ctgattgcaa tctccgcaat
     1801 cttctactct ctgttcagct aagtcctttc gcgccgcttt cgggcggcgc ttcctccgtt
     1861 ttaacgcgat gtatttccta tgattagcgt attcgatatt ttcaaaatcg gcattggccc
     1921 ttccagttct cataccgttg gaccaatgaa agcgggtaaa caatttaccg acgatctgat
     1981 tgcccgtaac ctgcttaaag acgtgacccg cgtggtggtt gacgtgtacg gctcgctctc
     2041 tctgactggt aaaggccacc acacagatat cgccattatt atgggcctgg cgggtaacct
     2101 gccggatacc gtggatatcg attccatccc cggttttatt caggatgtga atactcatgg
     2161 tcgcctgatg ctggcaaacg gtcagcatga agtggagttc ccggttgatc agtgcatgaa
     2221 cttccatgca gacaaccttt ctctgcatga aaacggtatg cgcattaccg cgctggcggg
     2281 cgataaagtc gtttacagcc agacttacta ctctattggc ggtggcttta tcgttgatga
     2341 agagcatttt ggccagcagg atagcgcacc ggttgaagtt ccttatccgt acagttcagc
     2401 agccgatctg caaaaacatt gtcaggaaac cgggctgtca ctctctggcc tgatgatgaa
     2461 aaacgagctg gcgctgcaca gtaaagaaga gctggaacag cacctggcga acgtctggga
     2521 agtcatgcgt ggcggtattg agcgcggtat ttccaccgaa ggcgtgttgc ctggcaaact
     2581 gcgcgttcca cgccgtgctg cggcactacg ccggatgctg gtcagccagg ataaaaccac
     2641 cactgacccg atggcggttg ttgactggat caacatgttt gcactggcag tgaacgaaga
     2701 gaacgctgct ggcggtcgcg tggtgactgc gccgactaac ggtgcgtgcg ggattatccc
     2761 ggctgtgctg gcgtactacg ataagtttat ccgcgaagtg aacgctaact cactggctcg
     2821 ttacctgctg gtagccagcg ccattggttc tctttataag atgaacgcgt cgatttctgg
     2881 tgccgaagtg ggttgccagg gtgaagttgg cgtggcgtgc tctatggcgg cagctggtct
     2941 ggcagaactg ttaggcgcaa gcccggcgca ggtttgcatc gcagcggaaa tcgcgatgga
     3001 gcacaacctc ggtctgactt gtgacccggt cgcggggcag gttcaggtac cgtgcattga
     3061 gcgtaacgcc attgcagcag tgaaagcggt gaacgccgcg cgtatggcac tgcgccgtac
     3121 cagcgaaccg cgcgtctgcc tcgataaagt tatcgaaacc atgtacgaaa caggtaaaga
     3181 catgaacgcc aagtaccgcg aaacctctcg cggcggcctg gcgatgaaga tcgttgcctg
     3241 cgattaatct ctcccagagg cctcgttttg cgaagcctct tcctgttttc tcaaccagcc
     3301 atagcctgtt ccggcatcga atgttaccct tatcgcctga tctttaaggg ggttatcgtg
     3361 gctgttcatt tgcttattgt cgatgcactg aatcttatcc gtcgcattca tgccgttcag
     3421 ggttcgccct gtgtcgaaac ctgccagcat gcgctcgatc agctcattat gcacagccag
     3481 ccaacccacg cggtcgccgt ttttgatgat gaaaaccgca gtagcggctg gcgtcatcag
     3541 cgtttaccag attacaaagc ggatcgaccg ccaatgccgg aagagttgca cgacgagatg
     3601 cctgcattac gcgccgcctt tgagcaacgc ggcgtcccgt gctggtcagc cagcggcaac
     3661 gaagccgatg acttagccgc cacactggcg gtcaaagtga cacaggccgg gcatcaggca
     3721 acgattgttt cgacagataa aggctactgt cagttacttt caccgacatt acgtattcgt
     3781 gattacttcc agaaacgttg gctggatgcg ccatttatcg ataaagaatt tggcgttcaa
     3841 ccgcagcagt tgcccgatta ctggggactt gcggggatca gcagttcaaa ggtaccgggt
     3901 gttgcgggaa tcggaccaaa aagcgccacg cagctgctgg tcgagtttca gagtctggaa
     3961 gggatatatg agaatctgga tgcggttgcc gaaaagtggc gcaaaaaatt agaaacccat
     4021 aaagagatgg cgtttctgtg ccgcgatatt gcccgcttac aaaccgattt gcatatcgac
     4081 ggcaatttac agcaattgcg gttggtacgg taacggcgag ccggatacgc cgcaaatcgt
     4141 atccggcatt atcacatcag cgcatttacc aggcggtatg gtaaagctct acaatatcct
     4201 caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc atccagtgcc gcctgagcca
     4261 gtgccgaaat gtcttccttg cgtacaccaa catcacgcaa atgtggcgga ataccgacat
     4321 cacggttgag ggcaaacacc gcttcaacag cggcattacg cgccccttcc aggctcatac
     4381 cttccacttt cacgcccata acgcgcgcga tatcgcggta cttctcaccg gtaaagtcag
     4441 cgttatagcg catgacatgc ggtaacagga tggcgttcgc aacaccgtgt ggagtgttat
     4501 aaaacgcgcc cagtggatgc gccataccat gcaccaaccc taacccaaca ttcgagaagc
     4561 ccatacccgc aacatactgc ccgagcgcca tttcttctcc ggcatcctta tcaccagcaa
     4621 ccgatcctcg cagcgcccca gcaatgattt caatcgcttt aatgtgcagt gcatcggtta
     4681 gtgcccacgc gccacgggta atatacccct caatagcatg agtgagcgca tcaacacccg
     4741 tcgcggcttt cagcgccgga ggcataccat ccatcatgtc agcatcaata aacgccacct
     4801 gcgggatatc atgcggatca acgcaaacaa acttgcgccg tttttcttcg tcagtgatca
     4861 cgtagttaat ggtcacttct gccgcagtac ctgctgtggt ggggattgcc agaatcggta
     4921 cactgggttt attggtcggg gaaagccctt ccaggctacg cacatcggca aactccgggt
     4981 tgttgctgat aatgccaatt gctttacaag tatcctgtgg agaaccacca ccaatagcga
     5041 tcaggtaatc cgcgccgcta ttctggaata caccgagccc ttctttgacg acagtaattg
     5101 ttgggttggg cactacgccg tcgtaaatcg cccatgccag ccctgcagca tccatcttat
     5161 cggtcacttt cgccaccacg ccgcattgca ccagcgtttt atcggtgacg atcagcgcct
     5221 tctgataacc acggcgtttc acctcatcgg ttaaagcccc aacagcaccc cgaccaaacc
     5281 atgccgtttc gttcagaatc attctgttag ccatcatcct tctccttgtt gctttacgaa
     5341 attactcttc aatccgtaac ccataggttt tgaatttctc cagcactacg gcaatctctt
     5401 catcgctcag cactggcacc gggtccgtaa tcgccagggt cgtcaggtaa agttgcgcca
     5461 gcacttcaac ttcatgcgcc agccataacg ctttttccag attcacctca caagcgataa
     5521 gcccatgatg ttgtaacaaa gttgccttac gatttttgag agccagcgca acatgttcag
     5581 aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg aatagaatta ccgccagccg
     5641 ccgcaatcat gtagtgaata gcggggatcg atcggttaag aatggaaact gccgtgcaat
     5701 gaacggcatg attgtgaaca accgcgttgg catccggtct gctttgatag gctgccatat
     5761 ggaaacgcca ttcgcttgag gggagctttc cttcctcatg tttaccgttg ccatcaataa
     5821 agacaatatg cgactccgtc agtttttcat atggaatgcc tgtaggcgta atcagcatcc
     5881 catcctgata acgtacactg acgttccccg ctgtcccctg gttcagtccc aggcgggtca
     5941 tttccaggca agtgtcaata atctgacgag caagtttatt tcgttccatt agctacctct
     6001 ctctgattca aaacagggca ataatgttgt tcctttcaca ctattgaatt agccgtttaa
     6061 ttacccacca tcttcttcct gattaacaag aaagaaattc acaagcttat attttgtgac
     6121 ctagctcaac taatcacagt aaataactgc aagttctctt tttataaccc cgttaaaaat
     6181 gaccgctctt aaaaatattt atcaaaacgg tcatttttcc atttcccctc gccagaaatg
     6241 cccgttttcg gcacaaacaa ttaatacggt catctggttt gtgtttttta tgatttattt
     6301 tctgaaacgg gcatgaaatt tcgattatta aagtgatggt agtcacataa agtcaccttc
     6361 cagctaataa gtgtgaccgc cgtcatatta cagagcgttt tttatttgaa aatgaatcca
     6421 tgagttcatt tcagacaggc aaatattcac cgatatgaag cccgaactcg ctggttttgc
     6481 acttttgaaa acataaccga ttacgtgctt aagcttctga acctaagagg atgctatggg
     6541 aaacacatca atacaaacgc agagttaccg tgcggtagat aaagatgcag ggcaaagcag
     6601 aagttacatt attccattcg cgctgctgtg ctcactgttt tttctttggg cggtagccaa
     6661 taaccttaac gacattttat tacctcaatt ccagcaggct tttacgctga caaatttcca
     6721 ggctggcctg atccaatcgg ccttttactt tggttatttc attatcccaa tccctgctgg
     6781 gatattgatg aaaaaactca gttataaagc agggattatt accggattat ttttatatgc
     6841 cttgggcgct gcattattct ggcccgctgc agaaataatg aactacacct tatttttagt
     6901 tggcctattt attattgcag ccggattagg ttgtctggaa actgccgcaa ccccttttgt
     6961 tacggtatta gggccggaaa gcagtagtca cttccgctta aatcttgcgc aaacatttaa
     7021 ctcgtttggc gcaattatcg cggttgtctt tgggcaaagt cttattttgt ctaacgtgcc
     7081 acatcaatcg caagacgttc tcgataaaat gtctccagag caattgagtg cgtataaaca
     7141 cagcctggta ttatcggtac agacacctta tatgatcatc gtggctatcg tgttactggt
     7201 cgccctgctg atcatgctga cgaaattccc ggcattgcag agtgataatc acagtgacgc
     7261 caaacaagga tcgttctccg catcgctttc tcgtctggcg cgtattcgtc actggcgctg
     7321 ggcggtatta gcgcaattct gctatgtcgg cgcacaaacg gcctgctgga gctatttgat
     7381 tcgctacgct gtagaagaaa ttctaggtat gactgcaggc tttgccgcta actatttaac
     7441 cggaaccatg gtgtgcttct ttattggtcg tttcaccggt acctggctca tcagtcgctt
     7501 cgcaccacac aaagtcctgg ccgcctacgc attaatcgct atggcactgt gcctgatctc
     7561 agccttcgct ggcggtcatg tgggcttaat agccctgact ttatgcagcg cctttatgtc
     7621 gattcagtac ccaacaatct tctcgctggg cattaagaat ctcggccagg acaccaaata
     7681 cggttcgtcc ttcatcgtta tgaccattat tggcggcggt attgtcactc cggtcatggg
     7741 ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa ctgatccccg cactctgctt
     7801 cgcggtcatc tttatctttg cccgtttccg ttctcaaacg gcaactaact gaacatattt
     7861 tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct taccgaaaat tggtatccgc
     7921 ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc ttgaagaaca aacaatgaat
     7981 atggcgaaag ctacggccgc actgctgacc gagaaactgc gccatgcctg cggagctgcc
     8041 gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg ctgaagccgc tgcttgcgaa
     8101 gaaaaattca gcagccagaa tgtaggcctc accattacgg taacgccttg ctggtgctat
     8161 ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag ccatttgggg ctttaacggc
     8221 actgaacgcc ccggcgctgt ttacctggct gcggctctgg cagctcacag ccagaaaggc
     8281 atcccggcat tctccattta cggtcatgac gttcaggatg ccgatgacac atcgattcct
     8341 gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg gtttggccgt cgccagcatg
     8401 aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg gtatcgccgg ttctattgtt
     8461 gatcacaact tctttgaatc ctggctggga atgaaagtcc aggcggtgga tatgaccgaa
     8521 ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg aactggaaat ggcactggcc
     8581 tgggctgata aaaacttccg ctatggcgaa gatgaaaata acaaacagta tcaacgcaat
     8641 gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga tggcgatgtg tatccgcgac
     8701 atgatgcaag gcaacagcaa actggccgat attggtcgcg tggaagaatc acttggctac
     8761 aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga ccgatcaata tcccaatggt
     8821 gacaccgccg aagcgatcct caacagttca tttgactgga atggcgtgcg cgaacccttt
     8881 gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa tgctaatggg tcaccagctc
     8941 accggcaccg ctcaggtatt tgccgatgtg cgtacctact ggtcaccaga agcaattgag
     9001 cgtgtaacgg ggcataaact ggacggactg gcagaacacg gcatcatcca tttgatcaac
     9061 tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg acagcgaagg taacccgacg
     9121 atgaagccgc actgggaaat ctctcagcaa gaggctgacg cttgcctcgc cgctaccgaa
     9181 tggtgcccgg cgatccacga atacttccgt ggcggcggtt actcttcccg cttccttacc
     9241 gaaggcggcg tcccgttcac catgactcgt gtcaacatca tcaaaggcct gggaccggta
     9301 ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg atgtgcatga catcctcaac
     9361 aaacgcacca actcaacctg gccaaccacc tggtttgcac cgcgcctcac tggtaaaggg
     9421 ccgtttacgg atgtgtattc ggtaatggcg aactggggtg ccaaccacgg agttctgacc
     9481 atcggccacg ttggcgcaga ctttatcacc cttgcctcca tgctgcgtat cccggtatgt
     9541 atgcacaacg ttgaagagac caaagtgtat cgtccttctg cctgggctgc gcacggcatg
     9601 gatattgaag gccaggatta ccgcgcttgc cagaactacg gtccgttgta caagcgttaa
     9661 tacattacac cctgatgtga tgcccggtcg ctccggctac cgggcctgaa caagcaagag
     9721 tggttagccg gataaacaat gttatccggc tatattgcag gagcgattat gaaacaagaa
     9781 gttatcctgg tactcgactg tggcgcgacc aatgtcaggg ccatcgcggt taatcggcag
     9841 ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg atatcgcgat ggaaaacaac
     9901 acctggcacc agtggtcttt agactgccat tttgcaacgc tttgctgatt gctgtcggca
     9961 aatcaatagt gaactgactg aatgccacat ccgcggtatc gccgtcacca cctttagtgt
    10021 ggatggcgct ctggtagata agcaaggcaa tctgctctat ccgatcatta gctggaaatg
    10081 tccgcgaaca gcagcggtta tggacaatat tgaacggtta atctccgcac agcggttgca
    10141 ggctatttct ggcgtcggag cctttagttt caatacgtta tataagttgg tgtggttgaa
    10201 agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg ctcttcattt cgtcgctgat
    10261 taaccaccgt ttaaccagcg aattcactac tgatatcacg atggccggaa ccagccagat
    10321 gctggatatc cagcaacgcg atttcagtcc gcaaatttta caagccaccg gtattccacg
    10381 ccgactcttc cctcgtctgg tggaagcggg tgaacagatt ggtacgctac agaacaacgc
    10441 cgcagcaatg ctcggcttac ccgttggcat accggtgatt tccgcaggtc acgataccca
    10501 gttcgccctt tttggcgctg gtgccgaaca aaatgaaccc gtgctctcct ccggtacatg
    10561 ggaaatttta atggttcgca gcgcctaggt tgatacttcg ctgttaagtc agtacgccgg
    10621 ttccacctgc gaactagata gccaggcatg gttgtataac ccaggtatgc aatggctggc
    10681 atccggcgtg ctggaatggg tgagaaaact gttctggacg gctgaaacac cctggcaaat
    10741 gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat ggtgtgaaaa tgcagtgtga
    10801 tttattgtcg tgtcagaacg ctggctggca aggagtgacg cttaatacca cgcgggggca
    10861 tttctatcgc gcggcgctgg aagggttaac tgcgcaatta cagcgcaatc tacagatgct
    10921 ggaaaaaatc gggcacttta aagcctatga attattgtta gtcggtggag gaagtcgcaa
    10981 cacattgtgg aatcagatta aagccaatat gcttgatatt ccggtaaaag ttctcgacga
    11041 tgccgaaacg accgtcgcag gagctgcgct gttcggttgg tatggcgtag gggaatttaa
    11101 cagcccggaa gaagcccgtg cacagattca ttatcagtac cgttatttct acccgcaaac
    11161 tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac aatttcgccg ttaatttctc
    11221 ccgaactatt gaaagtgctg gcagagatgg gacatggaga tgaaattatt ttttccgatg
    11281 ctcactttcc cgcccattcg atggaaccgc aggtgatccg cgctgatggc ctgttggtga
    11341 gcgacttgct ccaggcgatt atcccgttat ttgagctgga cagttatgcc ccgccgctgg
    11401 tgatgatggc ggcggtagag ggtgacactc tcgatcctga agtagaacga cgttaccgta
    11461 atgcgctttc actacaagcc ccgtgtcctg acatcatccg catcaatcgt tttgcgtttt
    11521 atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg cgaacgagcg aagtacggga
    11581 atattctttt aaaaaaaggg gtaacaccgt aatctcatac cggtacgccc gcatgacgcg
    11641 ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc acgccagcaa gcgatagtcg
    11701 acctgctgct gaaccatacc agcctgacca cggaagctct ctctgaacag ctaaaggtca
    11761 gtaaagaaac cattcgtcgc gatctcaatg aattacagac gcagggtaaa attctgcgca
    11821 atcatggacg cgctaaatat atccaccgtc aaaatcaaga cagtggcgat ccctttcaca
    11881 tcaggctgaa aagccattat gcgcataaag cagatatcgc gcgcgaggcg ctcgcgtgga
    11941 ttgaagaagg gatggtgata gccttagacg ccagttcaac ttgctggtat ctggcacgcc
    12001 agttgcctga catcaacatt caggtcttca ccaatagcca tccgatttgc catgaactcg
    12061 gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac acttgagcgc aaatatggct
    12121 gttacgtcaa tccctcgctg atttcccaac ttaaatcgct ggaaatcgat ctgtttattt
    12181 tttcttgtga agggatcgat agcagcggcg cactgtggga ctccaatgcg atcaacgctg
    12241 attacaaatc gatgctatta aaacgtgccg cgcaatcgtt gttattgatt gataaaagta
    12301 aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga tgaggtaacg cacattattt
    12361 ctgatgagcg ccaggttgca acttctttgg taacagcctg acggaaaggg tagcaggccg
    12421 gagacgacgc cccggccttg cctgttatcg ctcgtcgcga cgaccaccca ccgcagccca
    12481 gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga tacaactgcc gcgcctgaat
    12541 ctgagcattt atgccatgtt catcaagctg tgcctgaata tacgccagat tgtgtgatac
    12601 ttcttcgtag cgttttttca tcggcagttt gagattgaaa atggtttcac ggcaccagcc
    12661 attaaccagc cactgcgcca tcaatgccgc aactttagcc ggtttttcaa ccatatcgca
    12721 taccatccag gagatattgc tgcgtgtcgg acggaatttg aaaccgtctt cccgcagcca
    12781 cgtcacctgc ccggtatcca tcagactttg cgccatcggg ccgttgtcga cggaataaac
    12841 ccacatgttg cgcttcacca gttggtaggt ccagccgcca gggcaagcgc ctaaatccac
    12901 cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc gcaggaataa acacatgaaa
    12961 tgcctcttcc agtttgagcg tggaacgact cggcgcctct gccggaaatt tcaggcgcgg
    13021 aatgcccata tagaacggcg aattattgtt gctgtatgag taaccggtat agcagcatcc
    13081 tggtgcaata aagaatacat gcacaaccgg acgcttcggc gtttcatagt tcgccagcac
    13141 cccagcatcg cgcaaggcag cgcgtagcgg aacagtgaac ttacggcaga atttcagcaa
    13201 ctctttgctt tcgttggtat cggcaacttc aacacgcagt tcaccgccct tctctactac
    13261 accctgtaac atgccgacaa tgggggtaat acgatcttct ggcggcaaat gctgcaggag
    13321 ttcccccacc acaaaccact ggcgggcaaa aattaatgaa ctgaacggca gctcacggat
    13381 taacttatcg ccatcatcag gttgataaca ttcataaatg acataacccg cattctcttt
    13441 cacgcgggca aaaccgaaaa tttcccgctg gccggcttta tcggtaattt ctgcggcgca
    13501 ctctttttca aagcccggac ggcacagcaa tacaacctta ttcatgactt acgcccttgc
    13561 gctttaaacg gatagcacca actaacatta acgcccagcc cgcaaggaag ctcacgccgc
    13621 caaccggtgt gacaaacgcc cacaaacgca gatgggacag cgccaggcaa taaaggctgc
    13681 cgctgaacag caccgtgcct aacgcgagga aaacgctact ccagtaaaac cagatactga
    13741 tgcgacgctg cattgccacc gccagaccta agatcgccag cgtatgaaac gcctggtatt
    13801 cgagaccggt ttggatccag cccatctcaa cggcccccat ggttttactt aacacatgcg
    13861 caccaaaagc gcccagtgcc acaaaaatga agccgctaat ggcggcgaaa atcagcataa
    13921 aacggctggt catggtcgta ccctacgtaa attattgttc ataacgaaag cggaattttt
    13981 cttgttcagc agcggctttc gccaggatcc attggcgaaa ggcggctatt ttacccagtt
    14041 ctgcctggct gtcatgacaa accagataaa aagcattttt actgaccaga acatcattaa
    14101 acgggcaaac aagacgtccg gcctcgattt cagattgcgc catcacgtta tttgccagcg
    14161 ccactccctg cccgtgaata gccgcttgca gcaccatggc gctatgacta aaaattggcc
    14221 cttgctgaac gttgatatga ttaaacccca actgtcgggt atatgtctgc cagtcacggc
    14281 gtgacgcatc atgtaataac gtatgtttag ccagatcttc cggggtcttc aagggttttt
    14341 cgcctgtcag cagtagcggc gaacacaccg gcaataaata ttcggcgtac agtttttcca
    14401 cccgtagccc cggccagttg ccccgaccat aaaatatcgc cacatcaaca tcatccgcca
    14461 gcttatcttc ctgacgatca accgcctgga ttcgaacgtc aattcccgga taagctgaat
    14521 taaagctgga aagtcgcgga accaaccaat gaatggcgaa actggggagt aaactgaccg
    14581 tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt cgcttcggtt aattgcgaaa
    14641 atatctcttt gatatcgagg aaatagcttt gcccttcctc ggtcagcagg agtgaacgat
    14701 tacggcggcg gaacagtttt agccccaaaa aatcctcaag agacttgatt tgatgactta
    14761 ctgcggcctg ggtcacaaaa agctcttctg ctgcgcgagt aaaacttaaa tggcgtgctg
    14821 cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg tttagacatg gcttttaaac
    14881 tttgatgtta aatgaattta acaattagat cacactgtgt aacctattag tttttttaat
    14941 ctgagccatt ataaattgtc cgttgagctt ctaccagcaa atacctatag tggcggcact
    15001 tcctgagccg gaacgaaaag ttttatcgga atgcgtattc tggtgaactt ttggcttacg
    15061 gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga ttcagaccat ggtagcaaag
    15121 ctaccttttt tcacttcctg tacatttacc ctgtctgtcc atagtgatta atgtagcacc
    15181 gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat tactgttcca gttcgaccat
    15241 ctctttcacg tcagtacggt tgatctgctg tttgttgcca ttagcgtctt tatacgaaat
    15301 cataccgata tcgttatcag tctgtggttt gccgtcagag acgatggtac gtccgtcatt
    15361 ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg gcaaaagtca gcatacaggc
    15421 agaaataatt gcggcagtct ttttcatcat cttctcctta aaacgattaa taaatcttaa
    15481 tagtattgat taacaggcta aaattaacgc ctaacactat tcagcatatg ttacttacgc
    15541 ggcttcgcca ggatatccag ataattctga tggttagcac tctccttgta tcaaagtgaa
    15601 ttttgcgtca cgatcggtgc atcaagccga ggagtaccat gaacgttttt aatcccgcgc
    15661 agtttcgcgc ccagtttccc gcactacagg atgcgggcgt ctatctcgac agcgccgcga
    15721 ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca gttttacagc ctgagcgccg
    15781 gaaacgtcca tcgcagccag tttgccgaag cccaacgcct gaccgcgcgt tatgaagctg
    15841 cacgagagaa agtggcgcaa ttactgaatg caccggatga taaaactatc gtctggacgc
    15901 gcggcaccac tgaatccatc aacatggtgg cacaatgcta tgcgcgtccg cgtctgcaac
    15961 cgggcgatga gattattgtc agcgtggcag aacaccacgc taacctcgtc ccctggctga
    16021 tggtcgccca acaaactaga gccaaagtgg tgaaattgcc gcttaatgcg cagcgactgc
    16081 cggatgtcga tttgttgcca gagctgatta ctccccgtag tcggattctg gcgttgggtc
    16141 agatgtcgaa cgtgactggc ggttgcccgg atctggcgcg agcgattacc tttgctcatt
    16201 cagccgggat ggtggtgatg gttgatggtg ctcaggggac ggtgcatttc cccgcggatg
    16261 ttcagcaact ggatatcgat ttctatgctt tctctggtca caaactgtat ggcccgacgg
    16321 gtatcggcgt gctgtatggc aaatcagaac tgctggaagc gatgtcgccc tggctgggtg
    16381 gcggcaaaat gattcacgaa gtgagttttg acggtttcac cactcaatct gcgccgtgga
    16441 aactggaagc aggaacgcca aatgtcgctg gcgtcatagg attaagcgcg gcgctggaat
    16501 ggctggcaga ttacgatatc aaccaggccg aaagctggag ccgtagctta gcaacgcttg
    16561 cagaagaagc gctggcgaaa cgtccaggct ttcgttcatt ccgctgccag gattccagcc
    16621 tgctggcctt tgattttgct ggcgttcatc acagcgatat ggtgacgcta ctggcggagt
    16681 acggtattgc cttgcgggcc gggcaacatt gcgctcagcc gctactggca gaattaggcg
    16741 taaccggcac actgcgcgcc tcatttgcgc catataatac aaagagtgat gtggatgcgc
    16801 tggtgaatgc cgttgaccgc gcgctggaat tattggtgga ttaatgacaa acccgcaatt
    16861 cgccggacat ccgttcggca caaccgtaac cgcagaaacg ttacgcaata ccttcgcacc
    16921 gttgtcgcaa tgggaagata aatatcgcca gttgatcatg ctggggaaac agcttccggc
    16981 tttgccagac gagttaaaag cgcaggctaa agagattgcc ggatgcgaaa accgcgtctg
    17041 gctgggatat accgtggctg aaaacggcaa aatgcacttc tttggcaaca gcgaagggcg
    17101 cattgtgcgc ggcctgttgg cggtgttgtt gactgccgtt gaggggaaaa ccgccgccga
    17161 attacaggca cagtcgccgc tggcattgtt tgatgagctg ggattacgtg cgcagcttag
    17221 cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg attatcgctg cgacgaagca
    17281 ggtttaagca cagaaccggg tcggataaga cgttcgcgtc tcatccgacc tgattgttta
    17341 accctgacgc gccgctttcg ccatcatctt cttcagcgca tgagaaaccg caacaaaccc
    17401 aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat gcacaatcca tccgcttcgg
    17461 cccttctgcc gtggctttca tcgcacacac cgtaccgtct gactgcgggt acaccagcgc
    17521 ttcagtagaa aacacgcaat ccacgccgag cttaccttta ctgtttttca ctacgccaaa
    17581 atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga tcctgaatcg ttttcgccag
    17641 atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc gcgccaccgg tcgtgaccag
    17701 cgggatttta ttgcgccgac aataagcaat cagcgccgct ttgggccgta cactatcaat
    17761 ggcatcaatc acgtacgaat aaccggcact catatattgc gctacgttat ccggcgtcac
    17821 gaaatcatcc accaccgtta cacggcactc cgggttaatc tggcgaatgc gctccgccat
    17881 gacttccgct tttgccagcc cgacgttatc gcgcagagcg tgaatttgcc gattggtatt
    17941 ggtgacgcac acatcatcca tatcgataag cgtgattgcg ccaatccccg tgcgcgccag
    18001 cgcttccgcc gcccaggaac cgacaccacc gataccaacc acacaaatat gcgcgtcagc
    18061 aaacagttgc aacgcttttt caccatacag acgcgctgtg ccaccaaaac gctgacgcca
    18121 tgcatcacta attaccacag acatagcacc tcagaattaa aaagggcgaa gttatccctc
    18181 accctaaacg cataaatgcc tgatgcgcta cgcttatcag gcctacataa ctcctgcaat
    18241 attttggatt tgcatgattt gtaggccgga taaggcgttc acgccgcatc cggtatgaac
    18301 aacgcgcact tagtcaataa tcaggatgtg aggttacccc tcccctgtca tatccgtaaa
    18361 aacggcatac agaatatcac atcagccgct aaagacgtta cctgcgcccg gagccgtttt
    18421 cagcacccaa acccgtccat agtggttgta ccaacctgcg cggtgtccgg cttccggccc
    18481 gatcccttga tagatatcga agtgttggcc tttgattgcg cccccgacat ccaacgccac
    18541 catcagacgc aattcgtact gaccgttaaa tttgccgtta ttatccagca gcggcacttc
    18601 tgccagcaag gtagtacctg gcggaataat tgaacgatcg gaggcaacag aagcacggcc
    18661 aaccagcggc accgcacttg cccctttcac cggtgcaaaa gattgcggtt taaagaagac
    18721 gaaagacggg ttctgttcca gcagctcgcg aacctcggct tcactgtgtg tttcgcccca
    18781 atgacgaatc gcctgcatcg acatatcttc ttttttcact tcgccacggt cgatcagcac
    18841 cttaccaatg ctgcgatagg catgaccgtt tttccctgca tagctgaaaa agttaagcgg
    18901 actgccatca ccaaagtcga tatacccact accctgcaca tccataatga agttatccat
    18961 cagagagttg ctgtaagcga ggatgtattt atcgcccaat gccccggcgt agatctccgc
    19021 acgagacggc agacgaccac gttttggcgg catacggtaa ataggatact ggaactcgcc
    19081 ctggcgggta tggcgcgcct gaattaccgg cgtgtaatag cctgtaaact gcacgttacc
    19141 atagttgtcg gcaccttcca tctgccaggc atcaatgccg aactggcgca tattgcgggt
    19201 atcaccgcct gcgcgcagcc actcttgcac cgcgttataa acattactct ggttgccata
    19261 cagacgcggt gacgaattac ggatatggtt aatttgctcg gcaaaatcac cggcgttaat
    19321 cggcgcgcca acggcatctg gctggttcac cagagagaaa ggctgggtaa atttcccgtc
    19381 tttatattgc tgtccgcgat cggttggttt ggaagagcag gcggcaagca ttgccacaac
    19441 cgtgcccata agaaggtact ttacccaacg tcctttcatt gttctcttct tcaggttaaa
    19501 aaataaggcg caacgaagat aacaaaccgc cgcgtttaat gaaatgagca gcctctccct
    19561 gacgcaaatt ttgcacaaaa aacaggcttt agtggtttgt ttttgttcaa aatcatgcta
    19621 aatccgtgat cggggtaaaa aaaaggttgc atgaaaacgc gagcggagta tagtgcgcat
    19681 ccacggacgc ggggtggagc agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg
    19741 gttcaaatcc ggcccccgca accaattatt gaacacccta acgggtgttt ttttgtttct
    19801 gatctcccat aaaaaaagcg ccattcagcg cctttttatc atccccttct cgccagcgtt
    19861 gccccatcgg caaaatacgc tttaatcccc gcaagaatag actccgcaac ttcctgctgg
    19921 aaagtcgccg ttttcagttt acgctcttcc tcaacgttac tgataaacgc cgtttcgacc
    19981 agaatggagg gaatatctgg tgcctttagt acggcaaacc cggcctgttc aacttgattt
    20041 ttatgcagct tgttgatttt tccgagctta ttcagtaccg ctttaccaaa cttaaggctg
    20101 tcagcaatag tcagcgactg caccatatcg aacatggtgt ggtcgacata gcggtcaccg
    20161 cttttgctca cgccaccaat caagtccgag gcgttctggg tttgtgccag atattttgcc
    20221 gcagtactgg ttgcgccttt ggttgagagc gcaaacaccg aggaaccgct cggctgacga
    20281 ctggtaaagg cgtcggcatg gatagagaca aacaggtcag cacgctgttt ctgggctttt
    20341 gctacgcgca cttgcaacgg aatgaagatg tcttcattgc gcgtcatgta caccttcata
    20401 ttgccctctt cctcgatcag agagcgcaaa cggcgagcta tttgcaatac cacatctttt
    20461 tcgcgcgttt tgtatttccc caccgcaccg gagtcttcgc caccgtggcc agggtcaagc
    20521 ataatgacaa tcggacgatc gcgccctgct ttacccggtt gtggaccact ttgcgctggc
    20581 ggcacctgct tttcgaggtc gcctttgttg taatcctcca gcagcgccag cagcgggtcc
    20641 tgcatatcct gtgcattggc cggatagagg tccatcacca gacgctcttt aaacccggcg
    20701 accggcgcaa gggcaaacag ctgcggtttt acgttttgct ttaattcaaa aaccatgcgt
    20761 acggtttgcg ggtcaaattg cccgacgcgc gccgacttga tgaacgggtc gtctgcacga
    20821 atttgtgccg ccatcccctt gagtaccgag ttcaggttta catcttcgat atccaccacc
    20881 acgcgttcag ggttactcaa cgcgaactgc ttatatttca gctgacgatt tgattctacc
    20941 gtcacgcggg tgtagctgga cgcaggccag acgcgcaccg ccacgacctg gctaaccgca
    21001 gccaggctga cctgacttac gctcaatagc cacatagcgc ccgcgccttg cagtaaacga
    21061 cgacggctga ttgcagtgtt ggatcctgac atgcctctcc cgagcaaaag aaatctaagc
    21121 tgtgtaacaa gtaaacgact aatttgaccg gaaactttag cgaaagacgc ataatctgtc
    21181 atctaataaa cggtaaacat tctttttata ttcacggcat tactgataaa aaagtcgctc
    21241 tcgcataaaa tttacacttg caccctgcga aaaaacagaa taaaaataca ataatttcaa
    21301 ataatcatgc aaaggggtgt gccgtggtaa aggaacgtaa aaccgagctg gtcgagggat
    21361 tccgccattc ggttccctat atcaataccc accggggaaa aacgtttgtc atcatgctcg
    21421 gcggtgaagc cattgagcat gagaatttct ccagtatcgt taatgatatc gggttgttgc
    21481 acagcctcgg catccgtctg gtggtggtct atggcgcacg tccgcagatc gacgcaaatc
    21541 tggctgcgca tcaccacgaa ccgctgtatc acaagaatat acgtgtgacc gacgccaaaa
    21601 cactggaact ggtgaagcag gctgcgggaa cattgcaact ggatattact gctcgcctgt
    21661 caatgagtct caataacacg ccgctgcagg gcgcgcatat caacgtcgtt agtggcaatt
    21721 ttattattgc ccagccgctg ggcgtcgatg acggcgtgga ttactgccat agcgggcgta
    21781 tccggcggat tgatgaagac gcgctccatc gtcaactgga aagcggtgca atagtgctaa
    21841 tggggccggt cgctgtttca gtcactggcg agagctttaa tctgacctcg gaagagattg
    21901 ccactcaact agccatcaaa ctgaaagctg aaaagatgat tggtttttgc tcttcccagg
    21961 gcgttactaa tgacgacggt gatattgtct ccgaactttt ccctaacgaa gcgcaagcac
    22021 gggtagaagc ccaggaagag aaaggcgatt acaactccgg tacggtgcgc tttttgcgtg
    22081 gcgcagtgaa agcctgccgc agcggcgtgc gccgctgtca tttaatcagt tatcaggaag
    22141 atggcgcgct gttgcaagag ttgttctcac gcgacggtat cggtacgcag attgtgatgg
    22201 aaagcgccga gcagattcgt cgcgcaacaa tcaacgatat tggcggtatt ctggagttga
    22261 ttcgcccgct ggagcagcaa ggtattctgg tgcgccgttc tcgcgagcag ctggagatgg
    22321 aaatcgacaa attcaccatt attcagcgcg ataacacgac tattgcctgc gccgcgctct
    22381 atccgttccc ggaagagaag attggggaaa tggcctgtgt ggcagttcac ccggattacc
    22441 gcagttcatc acggggcgag gttctgctgg aacgcattgc cgctcaggcg aagcagagcg
    22501 gcttaagcaa attgtttgtg ctgaccacgc gcagtattca ctggttccag gaacgtggat
    22561 ttaccccggt ggatattgat ttactgcccg agagcaaaaa gcagttgtac aactaccagc
    22621 gtaaatccaa agtgttgatg gcggatttag ggtaagcgat gaaaatcacc ggatgcggca
    22681 ggcgtaacac tcgtgcgtcg catccggcaa ttacgtttat tcccgcgaac taaacaacgc
    22741 cgccagacca ctgcgccgct cagtacgagt ggcgattgcc gcacttaata tacgctcatc
    22801 ggcatacagc gacagacgac gacgcgcgcg ggtcaccgcg gtgtatacca gctctcgcgt
    22861 tactaccggc gtgcgttggc tcggtaaaat caacgccgca tggtcgaact ccgatccctg
    22921 cgatttatgt accgtcatcg cccacgtagt ttcgtgctct ggcaagcgac tcggttgcac
    22981 agacttaata ttgccgtccg gcatcgcaaa ccagacgcgc gtcccctgcc cgcgatccag
    23041 cgcaataccg atatcgccat taaacaaccc aagcgcgctg tcattacggg caatcatcac
    23101 cggtcggcct tcgtaccaac gagagtgcgg atgacgatga attttgcgct tctgttgcat
    23161 aaactgttca attcgctcat tcagtccagc cacgccaaac ggcccttccc gcagggcgca
    23221 caaaagctgg tactcattga acgcctgaat gattaaatcc ggctcggcac gcgcttgcag
    23281 cagatccaga taacgtccgt aacccgcaag agcttcctca agcatcgcaa tataatcttc
    23341 gccgctctgt aaaagtcgtt tttcgatatc agtaaaatcc tgctgaaaaa cggttttcac
    23401 tgccgtttta tcaccacggt tgatcgccgc agctaactga ccaatgccag aatcgctgcc
    23461 gaaacgatag cttttttgca gcaggcagag actgtcgcgc aaagatgccg cttctgtgcc
    23521 ggttcctgcc ggaacgtgag tccccgtcag gcggcttagc tgcctggcac gctcggcggt
    23581 aaagcccgcg ttggcataag cgcagatatc gcccagcaca gccccagcct caaccgaggc
    23641 cagttgatca cgatcgccga gaaagatcac tcgcgcatga tctggcaagg cgtcgatcag
    23701 tctcgacatc ataggcagat cgatcattga cgcttcatct accaccagca catcaagatg
    23761 cagcgggtta ccggcatgat gacgtaaacg ctggctaccc ggctgcgcgc ccagcaatcg
    23821 gtgcaaagtg ctggcatctt ccggaatgcg tttcttttgt tcatcggtca gcggtaactg
    23881 tcgcaaagcc ttgccgagag attcggttaa gcgcgcggca gctttacccg ttggtgcagc
    23941 cagacggata cggcagcgtt cgccgtcggc catttgaatt aacgctgcca gcaacttcgc
    24001 cacggtggtc gttttaccgg tgcctgggcc gccggaaatc accgagatcc gccgcgtcag
    24061 cgccactgcc gctgcaactt tttgccagtt aatttcatcg cttactggaa aaagtttgtc
    24121 cagggtttgc gccagtagag cttcatcaac ctcaatggta tgattcacct cattgaagaa
    24181 gcgcgctacc gtccgctcgt tacaccacat gcgattcaaa taaagacgat cgccacagag
    24241 gatcatcggc gtgggttcat ctcccctgct gaccgcttga gaagccagca agcattcttc
    24301 ccaattttgt agctcaccga tttcactgac acaggtcgcc aacagcggat tcgacgcctc
    24361 gttattttcc agtcgtgaaa gcggcaaaca gacgtgtccc tctccggcat catgacttaa
    24421 cagtgccgcc gcgagggtga cggcaggatg ttcatctccc gccacggtca gggcaaattg
    24481 cacatccagc gggcgtagct gtttgtgctc cacagcttcc agtaattgct tttgcaattt
    24541 cattacgcct cctccagggt cataccggca aacatctcat ccatcagggc aatcaacccg
    24601 gcgttgggtc gggttgtgta aatcccttgt tgcggatgtt ctttatcaac gccacgcagg
    24661 aacagataga taacgccgcc aaagtgaaga tcatagtcgt aatcagcaat gcgatggcgc
    24721 agataacgat gcagcgccag ggtataaagc tgatattgca gatcatagcg gtgtgcctgc
    24781 atcgctgctg ccatagcctg ttgggtgtaa gccgaactgt cttcacccag ccagttggat
    24841 ttatagtcga gcaggtaata acgcccttcg tggcggaata ccaggtcgat aaagcctttt
    24901 aacatgccgc gtacctgcat gaactccagc ggcgggcagc cagcggatag cgggtcaaac
    24961 tggcgaatta gtgcatcaag ctggctggcg atgagcggtt cgctaatcgg caggtaaaac
    25021 tccatctcca cctgtttatc gcggtcggaa agctgactca ggctaacacc cgtttcattg
    25081 agaggtgcct ggaggacagc cgtgatccac tcggtcaaaa ccggttccca ctgcgattca
    25141 aagccgccga gttccagttt ttcctgtacc cagttcgggt caaccggctg ggtaaaatcg
    25201 aggtcttcaa acaaactgtg caagaatgtc cccggtgacg caccgcgcgg gaactgatgc
    25261 ggtgttaacg tcggttcttc aacgacgctg accacgcctg cggcatcaac atccagccgt
    25321 ggcatcagat cctgagcgat accgtgacca cgctgctgca agccggagta gctggtgaca
    25381 cgccagttat cgccgggcaa tcgttgtagt gtcctcgcat tcagttctgc agttaaagca
    25441 tcattaacct gccagggctg gttatcacca gtttgtgccg tttgccaggc aatatcatca
    25501 tcgcataacg cttcaataca ggtgcgaagc cctgccgcat cttgcggttc ccctttttgc
    25561 agcaaacgcc cgagcgcact ttggtggacg tcggtgtcac cttttttatc gccacgacgg
    25621 cgcaccagcg gtgcaacgcc gagactgcaa tgccaaaccg aacgcgtcag cgccacgtaa
    25681 agcaaacgca gatcttccgc cagacgttcg acctccgcga ggtcgacgct ttctggcgca
    25741 gcattaagat ccaaaactgc ctcaaacgag tggcgatcgt gataaaacgc ctgatcctgg
    25801 acgcggaaat tggtaataaa tggcaaccag accagcggat attccagccc tttcgattta
    25861 tggatcgtga caatctgcac cagatgttta tcactttcga ggcgcatttg ttggctggag
    25921 gcattactgt ctggctcgag gatatgttgc gataaccagc gtaccagcgc atgttcactt
    25981 tccagctgcg ttccggcttc ttgaagcagt tcgctgatat gcaagatatc ggtaagacgc
    26041 cgctcaccgc ctgccgttgc cagcaagttt tctgcaatgt tacgcgccga catcagcgcc
    26101 cgcagcatcg gcataacgcc acgtttgcgc cagatttgcc gataaccatc gaactcttcg
    26161 actaccgcat cccacgcatg ttcgtcatta ttcagcgttt caatatccag cgcattcagc
    26221 cccatcattg acgttgccag cgcactgcgc agggtgttct cacgttcggg cgtcatcacc
    26281 gcctgcaaca accaaagcat ttcctgcgct tccagagttt caaaaacact gtcgcggttc
    26341 gaaaggtaaa cggaagggat ttccagcaac gttaaggcat cgcgcacctg ggcggcctcc
    26401 tggcggctgc gcaccagcac gctgatgtcc gaagcacgca ccggacgcgc gtcgtcgccg
    26461 ttcatcagca acgcttcgcc ccgctgtccg gcttgtagcc agtcgcggat ttgcgcagca
    26521 catacctgcg ccatggtact ttgatagtcg ccaacgccgc agctttcgcc ttccatcagc
    26581 cacattttca tcgcaggctg cgtttcacct ttaaatacaa aacgtaacgc ctgatttttc
    26641 ccggctgatt tcactggaat aaacggtatt tcgcgaaaca tgaacgtgtc atcagtctgg
    26701 ctgaaaagct tgttcacact gttcaccatt ccaggtgcgg agcgccagtt ggtgtctaaa
    26761 gtgtagtggg catgaacttc actacgcgcc ttcatataag tgaagatatc cgcaccccgg
    26821 aatgcatata tggcctgctt cgggtcgcca atcagcaaca atgcggtttc cggctgatgg
    26881 tgccagatac ggcgaaaaat tcggtactgc tgggggtcgg tatcctgaaa ttcatcgatc
    26941 attgccaccg ggaatcgcgt acggatcgcc gctgccaaca cctcgccgct ttcgctacgc
    27001 agcgcggaat cgagccgact taacatgtca tcaaaaccca attcgccacg gcggcgtttt
    27061 tcacgcgcta ctgtttcgcg gatctcagcc aatgcgcggg tgatcaacag atcgcggatc
    27121 gacaatggtt ctgcaagcag ttgatcgatc gcctcaaaca gtggatgtcg cggggtttcc
    27181 cccccggcct tcgtgcgatc ttctaagaaa cgctgggaga atttttccag cgactccggc
    27241 aactgataac tgtttgtctc ttcttctgcc caggcgctga tcttttcgat ccatttagcc
    27301 tgattgctac ggttaaactt gcgtcgatca ataccagaag attcgatcag cgcatccagt
    27361 tcacccactg cgtcgcgcca ctgctgtttt accgtatcaa tacgcgccac aatttgcgcg
    27421 tggcgggaag ccagcgtttc atcatcgggc ggcggtgctt tgataaccgg cggttcgcct
    27481 tgcagataac gattaatatc gcgcagcaac gcctgcggcc ctttccaggt ttcaaagacg
    27541 acctgggcta tttcacgcgg cagcgggtag cagtggcgac gccagaaatc ggcgcaggcc
    27601 tggtagcgta gcagagactc atcttcaatc agctgctgct caaacagcat gccggattca
    27661 aaggcattca ggttgagcat gcgctggcaa aagccgtgaa tagtaaagac tgccgcttca
    27721 tccatctgcc gttcggctaa caacaaccac tgcgcggctt gcgctttatc gtcgatctct
    27781 tccagcaggc gttcgtacag tggattgtcg gtggtttcac gcagacaggc gatgcgcaac
    27841 tcgtggatat tgctacggat acgaccgcgc aattctgccg tggcagcctc ggtaaaggtg
    27901 accaccagca gttcttcaac gatcagcggg cggggaaagg cggcggaacc gcctagtcca
    27961 agtaacaggc gcaaatagag cgccgcaatc gtaaaggttt tgcctgtgcc ggcagaggct
    28021 tcaatcaggc gctcgccctg taagggcaag cgcaaaggat ctagtgtctc ggcgacatca
    28081 ctcattcttt tcactcatca ggggcattgt ttgctgcaac gcgctgacgt tctcccacac
    28141 tttccagcct tcaggatgca catattcggc tttcccgttc tggctgccgg aaatctgcga
    28201 cagaatagcc atgccttgcg gctcgaccac cgcctgatgg aagaaatcag caagtttttg
    28261 cggcgtcagc agttttatct gggccacgat tttatcacgc gaatcgaagc gcatattgcc
    28321 gcgatcgaaa tctttactta acttcgatgc ttcttcgccg agcgtttgcg gtgcctgcag
    28381 catctgggta attaccgcct gctggatttg cgcaaactca tctggcttca tcgctcgcaa
    28441 ttttgcctct gcggttggga aaaacgcctt gtaacgctcc cacaagaatg aaggctgttt
    28501 atcattgctt tgcaaaagga agcccatgcc ccactgacgc cccacgctca ttggaaacgc
    28561 aaacacggca tagcccaatt gttcttcggt acgcaactga ttgtagaacc acggctgtac
    28621 gatctgcccc aacagagagc tataggctga gctggtgtat tcatcgtagc cagtcggtac
    28681 aaataccgct gccagtgcgg agtcggtgct gttaccggct ttttcaaaga tgacggattg
    28741 ttttttatcg accactacat ctttgtttcg acaccactct gaaccatcag cgcccaactg
    28801 tttttgcaca tcgcgtgcca gcgttgttgc ctgggcctcg gtcatgttgc cgataaccat
    28861 aaactctggt cgagcccctg attttaaggc gtcgcgatag gccagcacct ctttcaacgt
    28921 aatggagggc aaaattttac gccgttcatc tcgcgagaag tacggcactt gcgagagcat
    28981 ctgcgcgggc ataatcgcct gctcaaacgc tttacccttt tctgcggaat ccatcatctg
    29041 gttataccag gacttcgcct gctcaagctg ctcttccgta gcggtatagc taaagtaccc
    29101 ctcgagcaat gcctggaaca gctgcggcag acgctgggtg taaccattag cattaatcat
    29161 aaggccgttg ttagcgttgg tggaaaaact tatgccacca accgacgcct ggttgcttaa
    29221 ctgatcaagc gccagccctg cgagataatc attgagcgca aacatcacct gattgcgggc
    29281 gctgtccatg gctttcggat tacgcaaaat caggctgaca tcagctttgg gctcgctggc
    29341 aaaataacgg cttggcgcat acaccacgcg cagattcgac tcatcaacaa tcagctctgg
    29401 atggtcgtat ttcttctctg acttaatcag cgagaaatca tcaggaatat aagggttaag
    29461 ctctggcaaa gagagcgcaa tgtcggcggc ttttttctgc cagtcggcga aagtttgtgc
    29521 gctgatttta tcgacctgat acggcgcatc gacaaagtaa gccgttttgt tgtgcggctc
    29581 tttcgggctg atataccaga tacgcgcatt ctgcggcgtc atcatcgcca gacgttcctt
    29641 tactgcttta gcatcgtacc gatcggcaat attgactgca tccagcgtat gctcaacagg
    29701 aacgcgaatc atggtatctg ccagccattc gacgtaatcc atatcacggg tgatcgacgg
    29761 ataacggaag tcgatatcca gcacattcgc cagttcatcg aagtattgtt tatcaatgcc
    29821 tttttcacgt aacagattga gatagctaaa aattgccgcc acaacctgat cgcgattagc
    29881 caggccttta tcagttaaag acgcagagat cgctaatacg ccgctgttgc cgttgacgat
    29941 aggatcggag ttggcgctaa tgccctcaac taatccctgc ttttgcagcc agtcagaaag
    30001 tgtacctggg ctgcgattgc caatcagata ggtaatcaat tcatcggttt tactacggaa
    30061 cttcgctgag ttgttatcga tgcgaaactc aacgcgcaac actttacgcg gcagcgcagg
    30121 gacgtaatga atgataatgc ccttttgcgc gtcggtgact accggcacgg tgatttccgg
    30181 ttttttgctc tctttgttcg gcacgcgacc aaaggtgtcc gccgccattt ttgccaactc
    30241 cggcagcggt ttattactgt aaataaccgc cttcatcaaa ttggcggaat agtacttctc
    30301 gtggaaatct ttcagcgcct gctgcaccgg attaccaggt ttgtcgctta aagtttcgag
    30361 gttaccacca gaaaactttg aaccggggtg tgccgggtta atggtttctg cgctgacctg
    30421 tgccatgcgc atcccgtcac gcgtacgcgc catggttaat tcagcgttca ctgcattacg
    30481 ctcacgttcg gcgtatttct tgtcgagcaa gggttctgca atagcatcgg ccaggcggtc
    30541 taccgcaccg ggcaaggcgt cgttctcaac ttccagatag aaagccgtgc gatacggcgc
    30601 agtgctggca ttgtgactac cgccgtgcat tttgagatat tcggccagac tgtcagcctg
    30661 cgggtacttt ttcgacccca tcagactcat atgttcaagg taatgtgcca gcccctggta
    30721 cgcctcggga tcttccagcg acccaacggg cactaccagc gccgagagcg atttaactgc
    30781 ctgcggatca gaaaccagca agaccaccat accgttatcc agacgtatag cctgatactg
    30841 gcggttatct ttatcacttt tacggatggt ttcctgaatc ggctgccatc ccgtttctgc
    30901 ctgacttaat ggtgcccaga gggcaacaaa caacaataat gctttgaacc aggtgctgcg
    30961 gggcattcac ggacctcata atcaacttta ttttctggac agattcaaca ggttaacgca
    31021 aatcatctgc cagaatttaa tcttgtgctg cacgagtcag cctatgttta tataaccatc
    31081 agtccgtgac tggtgcgcat cataaagtaa gcagatagat tgcgcaattt ttatacaaca
    31141 ctcatgactg attaaagcga aacagcggta acaggaaacg ttgcgactgt tcaacgatgg
    31201 cctccattgt ctctggtgtt aattgccgcc agagccgttg ataccagata tcatcacctt
    31261 cgccacgcac catcatgttg ccttcgtaag cctgaaggaa tttcgtacgg gctttttgca
    31321 acgtggaatc gtcatccagc atggcatcgt tttgcgcgtc ataacaggtt tttagccacg
    31381 cgccgccact ttcaggtaac accagcaatg gcgcggacat tccttcacga tacccctcaa
    31441 tcagttgtga gaggtaatgc aaagcctgtt cggctgcaag cggcggaaaa cgccactcac
    31501 cgtctttgcg tagaaaaagg cgactttcgc cattaccacc gctggcacag tagacaaggt
    31561 gttccagcca aagttgcatt ccctgcgcca cacttaataa agagggacgc cagcgcaaca
    31621 ggccatccgg ctgcacctgc ggcaaccagc cagttatctg cacaccgttg caggcgagat
    31681 caatttccat actctgcccc ggctggcgac aggcaatgac tctgtcggca agctgctgca
    31741 tctcctggca ctgtgtttcc cagaaaattt caccaaaagc gccatacggt aaatcccctg
    31801 ccgcccggaa gcggcggaac aagcgttcgg catcatcctg ctcaaccagt gcattcaata
    31861 actgctgatt gatttgataa cggctaagtc cttccagaat aaatggctcg gtgtctggga
    31921 tttcgctgtc ttcagtatgg aagttcacct gcaaacgcat ctggaagaac gcccgcaccg
    31981 gatgtgccca gaatcgttgt agcgtttcca gcggcacggt ttccggtaag gtaaacggca
    32041 gcggctgaac aaattcagaa tgtgctttac cagcctggct ggccgcaggt agccattcac
    32101 gagcatagct ttgtcgttcg cctggctggt agttttgcgg atcaaacggc atccgggtat
    32161 ggaggcaagt aagatgcgct tttacccttg cctcgctttc atcacagttg agcgcttcat
    32221 cgcccggtag ataatgactt tgcccgatgt agtcgatcag ttcctgcacc agtaccgacg
    32281 ggaaacgttc actgttatcc tgaatggaac gcccgatata gctgatatag agtttttgct
    32341 gcgcggaaat taacgcttcc aggaacaggt agcggtcgtc atcgcgacgg ctacggtcgc
    32401 cacgcttcgg tttctggctc atcaggtcaa agcccaatgg cgcaagctga cgtggataaa
    32461 cgccgtcgtt cattcccagc aggcaaacca ctttgaacgg aattgaacgc attggcatca
    32521 gagtacaaat gttaaccggt ccggcgagaa aacgctggct gatacgttct tgatccagac
    32581 gctgtgccag ttcatcacgc aatagtgaca gcggcaccgc gtcgccatac tgcgcaccta
    32641 aaccttcggc gataatcgcc tgccattgtt gttcgatcag cgtcatcgcc gcttcggttt
    32701 ccgcatccgg caggaagaag gcgttgagca tatcgcgaca aaccggcaac cactcttcta
    32761 gcggacgctc ctgtgccagc ccgcgacgcc agatgtttag ctgcattagc agtgaagcca
    32821 gatgccccac cagttctgca attaagccgc tcgattcatc ataaggtaga accgattgcc
    32881 actcgccctg cgcgctctcc atcgcgtagc ccagcaacat acgcgtcagg ccaaatcgcc
    32941 aggtgtgttg tccggtggcg gggagttcca gctcgcgaac gttgtcgtca tctatccccc
    33001 aacgaatgcc ggattcgttg acccactggc gtaaataacg cagcccttct tcggtgatgt
    33061 caaaccgcgc cgccagcacc ggcacatcca gcagcgccag cacatcctct gacacaaagc
    33121 gactgtcagg cagtgataac aggctgataa acgcttccag taccggatgt gactgccgcg
    33181 cacgacggtc ggaaatggcg taaggcaggt aacgatccgc aggtgcgcta ccaaacactg
    33241 cctgaataaa cggactgtag ctgtcgatat cagccaccat cacgatgatg tcgcgcggag
    33301 taagtgtcgg ggcttcctcc agcatcgcca gcaagcgatc gtgtaaaact tcaacttcac
    33361 gctgcgggct atggcaaacg tggaatgtga tactgctatc cagtggatca agcgggcgtt
    33421 tgttatcgct acgggaaaac tcttcgatgt tcacacccgc aacggcgcgg ttttccagtt
    33481 ccagaatgtc agactgaatg ttatgcagca ggttatctgg cgtcacatcg acaaaagcgt
    33541 ccagctcctg gctgctctcc aggtcagaaa ggagataaat gtagtcgcgc ccaagcttcc
    33601 cccatgaggc cagcagcgag ttgccgacat cctgttcacc atcgctgtta aagagctgtc
    33661 cggcattttc gctatcgcga aatagcggca attcgcgatc ttcaaaactg tgtcgacgct
    33721 gacgagtcag cagtttcgcc agataagcag gatctttaat atcgccccag taataacggc
    33781 aggggttggt aaacaggaga tggatttcaa tatgtttacc cagcgcctgg agcgcctgga
    33841 gataaacagg cggtaacgcg gaaataccgc atataaagac gcgcgaaggt aaccccggcg
    33901 ggcaggtcgt cgcggactcc agcgtttcga taaagcgctg atagagattc gcgcggtgcc
    33961 agcgcggttg tccgagttca tgggtatatt ccaccagcgc cttccacaac ggggcttgcc
    34021 aggcctgtgc ttctcccagc ccttcaacca aatgtcctgt ttcccactgt gccagccagt
    34081 ccggacgata gaccagatac tggtcaaaca ggtccgccgc ttttgaggaa agctggaaca
    34141 gttttcgctt gtcgctatcg tcagtcagat aatgccgcaa cagggtaaag tcttcgcgct
    34201 ccagcaattg cggcagcaga gtcatcagtt tccagctcat gctctgtttg ttaaaggcgc
    34261 tctctttggg gatttccggt aacacccgga cgaacatatc ccagataaag ctcgctggca
    34321 gcggaaaatc aatgtttgcc gcaataccaa acttttgcga cagggtcatt tgcagccact
    34381 gcgccatacc ggtactttgc accagaatca tctctggttc gaaaggatcg tccagccgtt
    34441 cgcgttcgac aataaactcc atcaacgctt ccagcacgtc cagacgattg gaatggtaga
    34501 cccttaacat agcggctcct gactactgac aattcgggca atgcaggcgg gtcatctcgc
    34561 cttctctgcc cccgggtgaa actagcgtaa cgctgatgct gacacatccc gcctgcgatg
    34621 tctgcattcg gttgacctgc cagttggcag gtggcgaaat cgcgcgcagt tgcgtttgct
    34681 gccaaccatg ccgccagagc ttttggtact ggtttctgct ggcgagactg ttcattgatg
    34741 ttcgctggat acccgataac gcagtgacaa tcatcaccat cagcaccatc agcaccatca
    34801 gcaccatcag caccatcgca accattacct ccggcaggct aaagccttgt tgattcttca
    34861 gggaagctga cataatgccc cctctttcag cggacaaaaa tcgctccagc cgcgtggcga
    34921 aaaaacaatt tttccatcaa tgacttcgcc tgttcgccac aacgaaacgc cttcataacc
    34981 ggcaatcaat aaggcttcat tatctgccag taaacgcaaa caaacctggg catcggttcc
    35041 agcgtactgc gagcactgaa ctgctggctg cgtctgccag gagtgcattt ttccccacgc
    35101 cagcgccgac tgaacgatgg cctggcggcg caatgactga ctttccatgc tcacgcgaga
    35161 ggcaaaactg cgatcctgct gactcattcc ttgcaatagc aagctaccca aaaccagcag
    35221 catcaggacc agagccagtg acgaaacacc tttttcgcgg ttcacaggtt gaatcctgtc
    35281 acgctatagc tggcatccac cacggtttgc ggttcagact tactggcagc acgcatatta
    35341 accgtcaaca ccggcgagaa gccgctgaca tcctgacgta cgacctgaaa agtgtcgata
    35401 atgatggcat ccggattagt gactttatcc cagcccttac cttcacagga tgtcgcaccg
    35461 cgtagcgttt ccagcacatg ctccttcaga cgaaatccaa tctggtcgga ctcttttacc
    35521 ggttcgcgat cccagatacc gttactgttc gcatcccact gcacaatgat acagtcaccc
    35581 tgtccgacaa tttccagccc ttcgccggta cagctgccat gacaataacc cgccctctgg
    35641 agatgcttcg cgacggtaaa tacccgcagc cagatttcat cttccagtgc cagcttacga
    35701 gtgctcgtta aactttcacg ctgtaacgca ggcagaaagc gtgcagcccc cagcaacaat
    35761 acgctactga tcgccatagc aatcaacact tccagcagag aaaaaccttg ctcttttaca
    35821 ggcatccttc tgtttctcct tgctgataaa gccggagtct tccccacggc gaaaccacca
    35881 gccaccactc gcccgttgag tttttgaagc gaatatgcac ggcccatgcg gtattgcgca
    35941 ggccaaagaa agcaagcgaa ggtgtcaggt cgctcatttc gacttcgggc cagcgtggca
    36001 caaagaccaa tggtgaactg ccatgacagg tattggcccc agcagcggaa ctcacaaggc
    36061 accataacgt cccctccctg ataacgctga tactgtggtc gcggttatgc cagttggcat
    36121 cttcacgtaa atagagcaaa tagtcccgcg cctagctggc ggtttgccaa agccgttgcg
    36181 actgctgcca gtattgccag ccatagagtc cacttgcgct tagcatgacc aaaatcagca
    36241 tcgcgaccag cgtttcaatc agcgtataac cacgttgtgt tttcatgccg gcagtatgga
    36301 gcgaggagaa aaaaagacga gggccagttt ctatttcttc ggcgcatctt ccggactatt
    36361 tacgccgttg caggacgttg caaaatttcg ggaaggcgtc tcgaagaatt taacggaggg
    36421 taaaaaaacc gacgcacact ggcgtcggct ctggcaggat gtttcgtaat tagatagcca
    36481 ccggcgcttt aacgcccgga tgtggatcgt agccttcaat ctcaaagtct tcgaaacggt
    36541 agtcgaagat ggattcgggt ttacgtttga taatcaactt cggcagcgga cgcggttcgc
    36601 ggcttaattg cagatgagtt tgatccatat ggttgctgta caggtgcgta tcgccaccgg
    36661 tccagacaaa atcacccact tccagatcgc actgctgcgc catcatatgc accagtaacg
    36721 cgtagctggc aatgttgaac ggcaggccga ggaagacgtc acaggagcgc tgataaagct
    36781 ggcaagagag tttgccgtct gccacataga actggaagaa tgcatggcac ggtgccagcg
    36841 ccattttatc cagttcgcct acgttccacg ctgaaacaat aatgcggcgc gaatccgggt
    36901 cgtttttcag ctggttcagt accgtagtaa tctggtcaat atgacgacca tctggcgttg
    36961 gccaggcgcg ccactgttta ccatacactg gcccgaggtc gccattttca tcggcccatt
    37021 cgtcccagat ggtgacattg ttttcgtgta gataagcaat gttagtatcg ccctgcagga
    37081 accacagcag ttcgtggatg atggaacgca ggtggcaacg tttggttgtc accagcggga
    37141 atccatcctg caggttaaaa cgcatctgat gaccaaaaat ggaaagcgtt ccggttccgg
    37201 tacggtcgtt tttctgtgtg ccttcgtcga gcactttttg catcagttct aaatactgtt
    37261 tcatggttcc tcaggaaacg tgttgctgtg ggctgcgacg atatgcccag accatcatga
    37321 tcacacccgc gacaatcatc gggatggaaa gaatttgccc catgctgatg tactgcaccc
    37381 aggcaccggt aaactgcgcg tcgggctggc ggaaaaactc aacaatgatg cgaaacgcgc
    37441 cgtagccaat caggaacaag cctgagacag ctcccattgg gcgcggttta cgaatataca
    37501 ggttgaggat aataaacagc accacacctt ccagcagcag ttcgtaaagc tgtgatgggt
    37561 ggcgcggcag cacaccgtaa gtgtcgaaaa tggattgcca ctgcgggttg gtttgcagca
    37621 gcaaaatatc ttctgtacgg gagcctggga acagcatggc aaacgggaag ttcgggtcaa
    37681 cgcggcccca caattcaccg ttaataaagt tgcccagacg cccggcacca agaccaaacg
    37741 gaatgagtgg tgcgataaaa tcagagacct ggaagaagga acgtttagta cggcgggcga
    37801 agataatcat caccacgata acgccaatca ggccgccgtg gaaagacatg ccgccgtccc
    37861 agacacggaa cagatacagc ggatcggcca taaactgcgg gaaattgtag aacagaacat
    37921 aaccaatacg tcccccgagg aagacgccga ggaagcccgc atagagtaag ttttcaactt
    37981 catttttggt ccagccactg cccggacgat tcgcccgacg tgttgccagc cacattgcaa
    38041 aaatgaaccc caccagatac atcaggccgt accagtgaag cgccacgggt cctattgaga
    38101 aaatgaccgg atcaaactcc ggaaaatgca gatagctact ggtcatctgt caccacaagt
    38161 tcttgttatt tcgctgaaag agaacagcga ttgaaatgcg cgccgcaggt ttcaggcgct
    38221 ccaaaggtgc gaataatagc acaaggggac ctggctggtt gccggatacc gttaaaagat
    38281 atgtatatga tccgcgctat aaccctccgc gaatcagccc gcccatgccg cgacgctcca
    38341 taaaggctgc aacctgatga cgaacttcgg tcgccagttg cgcttccaga ctacgctgcg
    38401 caagattttc tgcttcggca aaatcaatgc gccgcagcag gtattttacc cgcgctacag
    38461 aacgtccgtt catagacaga tggcgatacc caagcccaat gaggattgcc acgcacatgg
    38521 gatcgcccgc catttcaccg cacaaacgaa gatcgatacc atgtatttcc gcttcccggg
    38581 cgatcatcgc cagagctcgt aacattgcag gatgaagact gtcgtaaatg ttcgccaccc
    38641 gggtattgtt gcgatcaacg gccagaatgt attgagtcag atcgttggtg ccaacagaga
    38701 tgaaatcgac cctctttgcc agatgcggca gcataaatac cattgacggc acttccagca
    38761 tgatgccgat acgtggtttg ggaatttcgt aaccgatcat ctcctcgact tcacgtccgg
    38821 cacgttcaat caggcggcgt gcttcgtcaa cttcatcgag gctggtgacc atcggcaaca
    38881 gaatattcag gttgcccgta gcggcattag cacgcagcat cgcccgcacc tggatcaaga
    38941 agatctccgg ctgatcgagc gtaatgcgaa tcccacgcca acccaggcat ggattctctt
    39001 cgctgatcgg catgtaaggc agctgcttat ctgctccgac atccagcgta cgcaaggtga
    39061 cgggtttatc attgaacatt tgcagcatcc cctgatactg cgccacctgt tcttcttccg
    39121 acgggaaacc actttgcagc atgaatggga tttcagtgcg ataaagaccg atgccatcaa
    39181 tacagctgcc cagtttttct tcatgttccg ggcttaaacc agcattgagc atgactttta
    39241 tacgctcacc gctttttaac tgcgcgggta aattgacgtc atcttccgcc agacggctaa
    39301 gctcaatctc ttcactaatt agccgctgat attcttgcag cagtaccggc tccggatcga
    39361 ccagcaattc accgcgatag ccatcaacga tcagcgtcct acgatgcagc accgaaggct
    39421 gaatatccgc gcccatcacg gtagggatcc ccagcgcacg taccatgatc gcagcatggg
    39481 agttggctgc gccatctcgc acgacaacac cgactaagcg atcctggggc agctcagcaa
    39541 gagttgtcgc tgacagttca tctgccacca gaatgaaacg ttccggccag gcgttcggcc
    39601 cttgattagc gtcatcaaga tgaaacagca atcgctgacc cagcgcacgt aaatcaccag
    39661 cccgctcttt gagatagtta tcgcttagcg cggcaaactg ttcggcaaat ttttcaatga
    39721 ccgtttttac cgcccactct gccaccgagc ctttatcaac ctcggcaaac aattcgcgac
    39781 gcagccgggt gtccgaaagc aggtgcgagt aaagatcgaa aatagccgcc gtttcttttt
    39841 gtgctccggc ggcaaagcgt ttgctgtagc ggcgaaactc gtttgccgct tcttccagcg
    39901 ccccggtcag tcgttcgcgt tccagagccg gatccagcgt tgatgcctga tacacctgtt
    39961 ccattaaagg taacgtggca tcctgccagc cttcggcaat cgccacacca ggtgctgccg
    40021 gtaatgcgcg gattcgcgtc tggcgatatt gcccaaacag ggcagtcaac tgcgactgag
    40081 aaagaatagc tgccatctgg gtggcaagcg tcaccaggaa ggattcttca ctttcgtcat
    40141 actggcgcaa ctctcgttgc tgtaccacca gtacaccaag caactggcga cgttgaataa
    40201 ttggtacgcc taaaaacgcg cggaaacgtt cttcttttac ggaggggatg tatttgaagc
    40261 tggggtgctt ttgcgcatct gcaaggttta tcggttccgc cagcctgcca accaggccga
    40321 cgatcccttc atcaaacgcg agcgttacag tgcgaccgcg tggttttttc agcccccggg
    40381 tcgccatcag gtagtaacaa cgtcgatcat gatcggccag gtagaccgaa cagacctcgg
    40441 tatccatcgc aagacagatg tcggtaacca gaatatttaa cgcctcattc aggcgtggtg
    40501 cgctggctac cttttcgact atttcgcgca ggcgagtgag cataattggc gtgacttaac
    40561 ctcttttacg tcgataagca gatgcgtttt gtggttttgg cgtattttcc tgcagtgaca
    40621 tcaccacact cgcgaactct ttcattaccc tacggtagac atcacgttta aatgacacca
    40681 cctgtctgac cggataccag taacttaccc atcgccagcc gtcaaactct ggtgtactgc
    40741 tggtttgcat attgatttct gcatcgccgc ttaccagctg caagagaaac catttttgtt
    40801 tttggccgat acaaaccggc ttcgtgtccc aacgcaccaa acgtttcggt aatttgtagc
    40861 gcaaccagtt acgcgttgaa gcaaggattc gaacgtcttt gcggcttaat cctacttctt
    40921 caaacaattc acggtacatc gcctgctctg cggattctcc ggggttgatt ccgccttgcg
    40981 gaaattgcca ggagtgctga ccaaatcgcc gggcccacat tacctgcccc tggcgattac
    41041 aaatcacgat acctacgttt gggcggtagc catcgtcatc aatcaccgga ctacctcaaa
    41101 ataaagcttt atatacgaat gattgtttca tactccagga agacggtaaa ccactctctg
    41161 cagggcatta cacactaata acaattgaat aactcacagt tatgtgcaga gttataaaca
    41221 gaggaagggg tggatagccg tttttattca ccttttctgt ggatagagtt gtgaagaact
    41281 acggaattac tacgggaaaa cccggagcat tctgaataag cccgatatag acattttaaa
    41341 atattcatta ttagtcaatg cattacatgt aatttgagta atgaaaaaga gtaattcgtg
    41401 acccaggtca cacctctcat ttacgggttg gcgaaagatc aaccaatgcc gtgtttatcc
    41461 acagaatgtg ccactaagtt aagcactgaa ccactaaaaa ctggagtttc gtcgcacgtc
    41521 aaggctgtaa atgaaaacag tagtggaggt ttttcacagt tatcccagct ttctgtggat
    41581 aacatggtgt aagatcctgt ttattttcag tgaccagatt tggaaaacct attgcagtgt
    41641 tgcgcaactc gattaccggc agcctaaaaa gcaatataaa tcagagaatt gaacaacaca
    41701 tgtggaaaaa gttacactgc gaatattcgg cacataattg ctgtttgttt tttaaccaag
    41761 gtatcatgac atgtcccaac ctcgcccact gctctctcct cccgaaactg aagaacagtt
    41821 gttagcgcaa gcacagcaac tttctggtta tacattggga gaactggcgg cacttgacgg
    41881 gctggttacg ccagagaatt taaaacgcga taagggctgg attggcgtgt tactggagat
    41941 ctggctaggt gccagcgcag ggagtaaacc tgagcaagat tttgctgctc tgggcgtgga
    42001 acttaaaact atccctgtgg atagtcttgg tcgtccgctg gaaacaacat tcgtttgtgt
    42061 tgcccggtta acgggtaata gcggggtgac ctgggaaacc agccatgtgc gccacaagct
    42121 caaacgcgta ctgtggatac cggttgaagg cgagcgcagc atcccgctgg cgcagcgtcg
    42181 cgtaggatca ccgttgctgt ggagcccgaa tgaagaggaa gaccgacagc tacgcgaaga
    42241 ctgggaagaa ttaatggata tgattgttct cggtcaggtt gagcggatca ccgctcgtca
    42301 cggggaatat ttacagataa gaccgaaagc agcgaatgcg aaagcgctta ccgaagccat
    42361 tggtgcccgg ggcgaacgga ttctgacgct gccacgcggc ttttatttga agaagaattt
    42421 caccagtgca ctactggccc gtcattttct gatccagtag ccatcgcttt gacctgccgc
    42481 tttccgggca tataattacc gcttcatttt tttggcaggg ctttttagat gttatttgca
    42541 tggataaccg atcctaacgc ttggcttgcg ctcggtacgc tgacgctgct ggagatcgtt
    42601 cttgggatcg acaatattat tttcctttct ctggtggtgg caaagcttcc cacagcacaa
    42661 cgtgctcatg cgcgccgtct ggggttggcg ggagccatgg ttatgcgtct ggcgctgctg
    42721 gcatcaatct cttgggttac gcgcctgaca aatccgcttt ttacaatatt cagtcaggaa
    42781 atttctgccc gtgatttgat tctgcttctg ggtggcttgt tccttatctg gaaagccagc
    42841 aaggaaatcc acgaatccat tgaaggtgaa gaagaagggc tgaaaacacg cgtttcatca
    42901 ttcctcggcg ctatcgtgca gattatgctg ctggatatta tcttcagcct cgactcggtg
    42961 attaccgctg tgggtctgtc agatcacctg tttattatga tggcagccgt ggtgattgcc
    43021 gtaggcgtga tgatgttcgc cgcgcgctcg attggtgatt ttgtcgaacg ccatccttcg
    43081 gtaaaaatgc tggcgctctc tttcctgatt ctggtgggct ttaccctgat tctggaaagt
    43141 ttcgacatcc acgtaccgaa aggttacatc tacttcgcga tgttcttctc tattgcggtt
    43201 gaaagcctca acttgattcg caacaaaaag aatccgctct gatactccgt acgctctcct
    43261 gcgggagggc gtactcactc cttttaccct cacagtttaa gattttcctg ctttcaagat
    43321 atataacgtc ggtttataaa cagactatta tcataggtag gccaaatgag gataaccgga
    43381 tgaaaaaatg ggcagtaata atttctgcag tcggactggc gtttgctgtt tccgggtgtt
    43441 ccagtgatta cgtcatggcg accaaagatg gccgtatgat tttgaccgat ggaaaacctg
    43501 aaattgatga tgataccggg ctggtgagtt atcacgatca gcaaggtaac gcgatgcaaa
    43561 ttaaccgtga tgatgtttcg caaattattg aacgttaaca aataaggtca gcatccggct
    43621 ggccttaaga tttttctctt tcccttttcc cttccctctg ccatttttat attccttatg
    43681 tcgtgattat aaaaaggaaa cggctatgca atatcaccgt ataccccaca gttcgctgga
    43741 agtcagcacg ctggggcttg gcacgatgac gtttggtgaa cagaacagcg aagccgacgc
    43801 ccacgcacaa ctcgactatg ccgtcgctca gggcattaac cttatcgacg ttgccgaaat
    43861 gtacccagta cctccgcgcc ccgaaacgca agggttaacc gaaacctacg tcggcaactg
    43921 gctggcgaaa catggcagcc gcgaaaagtt aattatcgcc tccaaagtga gcggaccgtc
    43981 gcgcaataat gacaagggca tccgcccgga tcaggcgctg gatcggaaga atatccgcga
    44041 agcgctgcat gacagcctca agcgcctaca gactgattac ctcgatcttt atcaggtgca
    44101 ctggccgcag cgcccgacca actgcttcgg caaactcggt tatagctgga cggattctgc
    44161 gcctgcggtt tcgctgctgg atacgctgga cgcactggca gagtaccaac gcgcgggaaa
    44221 aattcgttat atcggcgtgt cgaacgaaac tgcatttggc gtaatgcgct acctgcatct
    44281 ggcggacaaa cacgatctgc cgcgtattgt caccattcag aacccttaca gtctgttaaa
    44341 ccgcagtttt gaagtaggtc tggcagaagt cagccagtat gaaggggtcg aactgctggc
    44401 ctattcgtgc ctgggtttcg gcacgctgac cgggaaatat ctcaatggtg caaaacccgc
    44461 tggcgcacgt aatacgctct ttagtcggtt cacccgctat agcggtgagc aaacgcaaaa
    44521 agccgtcgcg gcgtatgttg atatcgccag acgtcatggc cttgatcctg ctcagatggc
    44581 gctcgcgttt gtacgccgtc aaccgtttgt tgccagcact ctgctgggcg caaccacgat
    44641 ggatcagctg aaaactaaca tcgaaagttt gcatctggag ttaagcgaag acgtattagc
    44701 tgaaattgaa gcggtacatc aggtttatac ttatcctgca ccataaagat taacgccggt
    44761 taaaaccggc gttaaatatt aatgacggcg ctgccagatc cacagcgccg ttattgccag
    44821 cgcaaatagc gcaccgaagc caatgccaat gggcacgacc gggatgccta ccattaccgc
    44881 cagcgagtaa atgcccagca tcaacaacat ggcgctgttt tcgccaaggt tttgtactgc
    44941 aatcgcattc cccgccccga cgcttttttt accccgctcc tgtagcaacg cattgagcgg
    45001 aacgacaaaa aagcccccca gcacgccaat cagcatcaac aaggcatagg ctggcagcag
    45061 ctcgtgttgc agggagaaaa tcggtaccac cacgccaatc aaaatcccgg ctggcataca
    45121 gcgtgacacg gtttccagcg taaccagctt cgctgccgca cctgcgccaa ccacgatacc
    45181 aatcgctacc atcgcgttga gataggtcgg cgtagcgtta tcggtaatgc ccagcgccac
    45241 cggtacccac agcaccaaca ggaaacgcag cgtgacaccc gctccccaga ataaactggt
    45301 gcccaccagc gaaaaacgcg tttcaccatt gcaccatagc gaggtgcagg cattcaggaa
    45361 actgcgggtc atgttgatga gattccagga ctgccccgga cgcgccgcca gtttgggaat
    45421 gtagatattg gcaacgaccg caccaccgta ggccagtgcg catgcggcca gagcgaccag
    45481 gacatgccag tcagccagca caccaccggc tacggaaccg agtaaaatcg ccgctatggc
    45541 agaagcttcc attaaaccgt tagcttttac taacttacta cccgtggtta attcgccgag
    45601 aataccgtat ttcgccggtg aataggctgc agcaccaaca cccaccagcg tatagccgag
    45661 aaacggattg ataccaaagc agatactggc tgcgcccagc agcttcaggc cgttggcaaa
    45721 catcatcacc cggcctttgg cgaagctatc cgccacctgc ccgacaaacg gcgccaaaag
    45781 aatgtaagca cctacaaaca ccatttgcag gatgggctgg ctccactccg gatagaactg
    45841 cgctttcagt aacgccagag tggcaaacag tagcgcgtta tcgccaaacg cagagagaaa
    45901 ctgcgccacg ataaccgctt tcatcccctt cgaccacaac gaagtgttag tgtgcactga
    45961 ctcactcatc gtgttgttcc gcttcgtcta cccagctttt caacgtgaca aagtcaggtt
    46021 tgccgctgcc aagtaatggc atctgtttca gatagcgaat atcgcgcggt acagcaagct
    46081 ccggcacgcc gtgctcgcgg gcatactgtt gcaacttatc gcgcgtcagt tcgttatctg
    46141 tggtgaaaag caccagtgcc tcgcctttgc tggcatcgct cttaatcgca gtggcatgga
    46201 ctttatctgg cgaaacacca agtgccagtt gttccaccat ttccagcgac accatttcgc
    46261 ctgcaatttt ggcaaagcgt tttgcgcggc cctgaatctg cacaaagccc tgctcgtcaa
    46321 aacgcacaat atcgccagtg tcataccagc cgcgctccat ttcgccgcga acattctcgg
    46381 cggtgggcac ttccagtaca cctggcttct ccacccgcag atagccgttc attatgttcg
    46441 gccctttcag ttgcaggcgt ccgccctctt cgataccagg gaccgacaac aggcgtgcat
    46501 ccattcctgg tagaatacgc cctaccgtac cgggtttcgc cgccatcggt acgttgatag
    46561 aaacgacagg cgcgcattcg gtcacaccgt agccttcaag gatgcgcagg ccaaatttat
    46621 cctgccaaag ctgtttggta ctttcttgta gtttttctgc gcctgccacc acatagcgta
    46681 gacgatagaa gtcatacggg ttggcgaaac gcgcgtagtg accgaggaaa gtcgaggtac
    46741 cgaacaacac ggtgcaactg cggtcataca ccaagtccgg cacaatgcgg taatgcagcg
    46801 ggcttggata aaggaacact tctgcacctg taagcagtgg cgtaaacagg cctaccgtca
    46861 gcccaaagga gtgaaacagc ggtaatgccg acataaagcg atcgttggtg gtgaagtcgg
    46921 caatcgtttt aatctgctcg acgttcgcca gaatgctttt atggctatgg acgacgcctt
    46981 ttggatggcc ttcagaaccg gaggtaaaga ggatcagcgc ctcttcttcc ggctgctgtt
    47041 ttacctgcgc caggcgcggc atcagcaaat gagcgaagat ccatactttg tcggcagtgg
    47101 tgacatctgc ttttaaatct tccagataga cccagcgcac ctgagtaagt tgctccggca
    47161 gatgccagag tttgccttta tcgagaaact ggcgggaagt gaagatggtt ttgatttcag
    47221 ccgccgtaat agcactggtc agccctttta ccccggcagt gtagttcatc attgcgggaa
    47281 tgcggcgacg cgcgatggcc ccaaaaatca ctgccgcact gatgcctgca ttgggcagca
    47341 ttaagccgat gcgttcgcct tcaacactgt atttttcaag gatgcgtcca acaaacagcg
    47401 ttttcgtaag caatttgcga taggagtctg gggtaaagtt gacgtcttcg acacatttct
    47461 tcccggctcc gaagcggtac attgcactca gtaaagattc gtacagagtt tcacgcgggc
    47521 gcaccgccat acgcgcttcc atcattattt gatgcagcat ttcgccagcg attttgcgac
    47581 ggtcacgggc acgcggcgca tccggcatcg ccacctgcgt tggaggcaaa atatgcagag
    47641 taatttgcgg gaacaagcga cgtttaacca gacctttcag gcggctgaag tgcgtaagtt
    47701 ccgccccttc aatacgcaca ggaataaccg ttgcaccaga cttcgccgcg acaaaacccg
    47761 cgccatcgta gattttcatc agcgagcctg tagtggtgat gcgtccttca gggaaaatca
    47821 ccactggtcg gccctgttcc accagacgta ccagatgttt aatagccata ggttgcgtcg
    47881 ggtcgagagg aacaaagtcg ataaatgatt tcagccaacg catataccac tgttggctta
    47941 ttgaggtgta aacggcaaac actggacgca caggtaaaaa cagtccaagc aaaatgccat
    48001 caataaaaga gacgtgatta ggcgtaatta gtacgcgctc gcccttcagt gcctgggtgt
    48061 cacccgtaac gcgaacgcga tacaaaacac ggcacaaatt tcgaaaaaag ctaaaaagca
    48121 ttccaactcc ctttgctctg attcagtaaa agcgaatgga gggagattac acgagataaa
    48181 gaacgcgagc gacagtaaat taggtgcgaa aaaaaacctg cgcatccgcg caggttggtg
    48241 caagagacag ggtacgaaga gcgtaccgaa taatctcacc aatcaatacc tctgggatct
    48301 tgattgtggt ctgcacgacg actcttcgcc agcgagaaaa cgcaaaggaa tgaagggaaa
    48361 tgcaacgagg tgtgtaaatt gtcggttact gttacagatt gatgaccggc aaaaaaaaac
    48421 ctgcgcatct gcgcaggctg gtgtaattca tgtgctcaac acgaagttga cttcacctat
    48481 caatacctct gggatcacca ctttagcaac ctgaagccaa acgccaccag cggtcaatcg
    48541 caacagcgtt tcgcaaagtg taaccaaagg tttgcaatct ctttttttgt cttacttatt
    48601 tgcgtttggc tcacattccc acgatgaaaa cacgccaccc cttgaaccaa cgggcgtttt
    48661 ccgtaacact gaaacaatgt aagcgtttac ccactaaggt attttcatgg cgaccataaa
    48721 ggatgtagcc cgactggcag gcgtttcagt cgccaccgtt tcccgcgtca ttaataattc
    48781 acccaaagcc agcgaagctt cccggctggc tgtgcatagt gcaatggagt ctcttagcta
    48841 tcacccgaac gccaacgccc gtgcgctggc gcagcagacc actgaaacgg tcggtctgat
    48901 cgttggtgat gtttccgatc cgtttttcgg tgcaatggtg aaagcggtcg aacaggtggc
    48961 ttatcacacc ggtaatttct tattgattgg caacggttac cacaacgaac aaaaagagcg
    49021 tcaggctatt gagcaactga tccgccatcg ctgtgctgcg ttggtcgtcc atgccaaaat
    49081 gatcccagat gccgatttag cctcattaat gaaacaaatg cccggtatgg tgctgatcaa
    49141 ccgtatcctg cctggctttg aaaaccgttg tattgctctt gacgatcgtt acggtgcctg
    49201 gctggcaacg cgtcatttaa ttcagcaagg tcatacccgc attggttatc tgtgctctaa
    49261 ccactctatt tctgacgccg aagatcgtct gcaagggtat tacgatgccc ttgctgaaag
    49321 tggtattccg gccaatgatc ggctggtgac atttggcgaa ccagacgaaa gcggcggcga
    49381 acaggcaatg accgagcttt tgggacgagg aagaaatttc actgcggtag cctgttataa
    49441 cgattcaatg gcggcgggcg cgatgggcgt tctcaatgat aatggtattg atgtaccggg
    49501 tgagatttcg ttaattggct ttgatgatgt gctggtgtca cgctatgtgc gtccgcgcct
    49561 gaccaccgtg cgttacccaa tcgtaacgat ggcgacccag gctgccgaac tggctttggc
    49621 actggcggat aatcgccctc tcccggaaat tactaatgta tttagtccga cgctggtacg
    49681 tcgtcattca gtgtcaactc cgtcgctgga ggcaagtcat catgcaacca gcgactaacc
    49741 gcagttaaag caattccagc gccagtaatt cttcgatggt ctggcgacgg cgaatcaacc
    49801 gcgcctgacc attatcaaac agaacttctg gtaacagcgg acggctattg tagttggatg
    49861 acattgatgc gccatatgcc cctgtatcat gcagtaccag ataatcacct gccttcactt
    49921 ccggcaaggc gcgggtttca acatttcccc cttcctgctg ggtaaagaca tcgcccgatt
    49981 cacataacgg cccggcgacg acggtttcca ccgttggcgc gtgttccaga gaacgaccat
    50041 cagctgccag ggcactgata tggtggtaac taccgtacat tgccgggcgc atcagatcgt
    50101 tgaacccggc atcaaccagc acaaagtggc ggctacccat ttgtttgacg ctccgcacct
    50161 gagtaattaa tacgccagac tgcgctacca ggaagcgacc cggttcaatt tccagtttca
    50221 cagggtggcc caaatggcga gcgatttgct cacgcgcggc attccacaga ccataataat
    50281 gttcggtatc aaccgcctct tcaccctgtt gataaggaat agaaagccca ccgcccgcag
    50341 aaatagcctg taaatcctga ccgaattcga taacctgacg caccatagca ccacacacct
    50401 gctccagatg ggcataatca acgccagaac caatgtgcat gtgaatgccg actagctgca
    50461 gatgatgacg ttgtatcacg tccagtgcgg cgggcagatc ggtgtaccag ataccgtgct
    50521 tgctgttttc gccaccggta ttggtttttt ggctatgtcc gtgaccaaac cccggattaa
    50581 cgcgcagcca tacccgatgc cctggcgaaa cctggcccag ttggtcgagc gtatcaacag
    50641 aacccgcatt caccggaatt tgcaattcac tgacgcgttc aagcgtcgcc tgatcgataa
    50701 catctgccgt aaaaacaata tcatcggggt gcgtttgcgg attgtaaccc gccgccaacg
    50761 cacgctctat ttcgcctaac gagacggaat ccactttcac gccctgctca cgcattaagc
    50821 gcaaaatatg aatattggaa caggctttct gtgcaaagcg caccacttca aactgtttca
    50881 gcgctgcaat ctgccgacga ataatttgcg catcgtagac ccacaccggg cagccaaatt
    50941 cagcgggcaa acgcagcaga ttttcggcgg tgagatcggt atcggtgctg aacagtgaat
    51001 gtggcataac aaactccaga tgagtgcttt tttatgatta cgccacatca taaaaagaat
    51061 aaaaaatatc gatttatgtc gagtctatgc aaaaatgata tggattaccg gattgcgaga
    51121 gagcgctaat ggccgccgtt aacttacgtc atattgaaat ttttcatgcg gtaatgaccg
    51181 ccggaagcct gactgaggca gcacacctgc tacacacctc acagccaacc gtcagccgcg
    51241 aactggcgcg ctttgagaag gtgatcgggc tgaaattgtt tgagcgcgta cgtggacgat
    51301 tacatcctac cgtgcaagga ctgcgtctgt ttgaagaagt gcaacgatcc tggtacggac
    51361 tggatcgcat tgtcagcgcc gcagaaagtc tgcgcgagtt tcgccaggga gaactgtcta
    51421 ttgcctgcct gccggtcttt tcgcaatctt ttttaccgca gctcctgcaa ccctttctgg
    51481 cacgttatcc cgatgtcagc ttaaatatcg tgccccagga atcaccgcta cttgaagagt
    51541 ggctctcggc ccagcgtcat gatttaggac tcactgagac actccatacg cctacgggaa
    51601 cagaacgtac cgaattactc tctttagatg aagtgtgtgt gttacctccg ggtcatccgc
    51661 tggcggtaaa aaaggtatta acgccggatg attttcaggg tgagaattac atcagccttt
    51721 cccgtactga cagctatcgc cagttgctgg atcagctatt tactgaacat caggttaaac
    51781 gacgcatgat cgtagaaacc cacagcgccg cgtcagtctg cgcaatggta cgggcggggg
    51841 taggtatttc ggtggttaac ccgctcaccg cactagatta tgcggcaagc ggtttagtgg
    51901 tgcggcggtt cagtattgcg gttccgttca ccgtcagcct gatccgcccc ctgcaccgcc
    51961 cgtcatcagc gctggttcag gcgtttagtg ggcatttaca agcggggtta ccgaaactgg
    52021 tcacttctct tgacgctatt ttgtcgtcag ctacgacagc ataaaagcga cagcatcctc
    52081 ggcatggatc gccgcggtat caaacacagg caaaacactg cgctcttctg gcaccagtaa
    52141 accaatttct gtgcagccaa aaatgacgcc ctgtgcgccc tgttctgcaa ggcgagcaat
    52201 cacttgcgca taataagcgc gtgacgcttc ggtaaattgc cccagacaca gttcttcaaa
    52261 aataatctga ttaattttcg cccgttcatc cgcttcagga ataagacaat tgatggaaaa
    52321 ttgttccgtc agccgcccgc gataaaaatc ctgttccatg gtgtaacgcg tacccagcag
    52381 cgccacacga gtcattccgg ccccggtaat tgcacgtccg gtggcatccg caatgtgtaa
    52441 gaaaggcaga gtgcaacgtg actcaatggc atccgccact ttatgcatcg tattggtaca
    52501 taacacaata ccttctgcgc ccgcccgctg taagccaagc gccgcctcag ccagaatgtc
    52561 cccggtttta tcccattccc cgcgacgctg gcactcttct atttcatgaa aatcgacgct
    52621 atgtagcagc atttgcgcag agtgaagccc accaagccgc tgtttaatgc cttcatttat
    52681 cagacgatag taaggaatgg tggattccca gctcattcct cccagcaaac caattgtttt
    52741 cattcctctc tcctgatgtg tgttaccgca acaaggctga ataaggaacg agacaaacgc
    52801 ctcaacggcc aggtgcccaa tctctattaa cgaaaaaagg gccggatgta cagcacatcc
    52861 ggcccgtgaa atcagacgcc gatatttctc aacttctcgc ctgccatcag tttgcgttcg
    52921 atatgttcca gcgtgacatt tttggtttcc ggaatgagcc agaaagtaat acccacaaac
    52981 gcaatgttca gcgcagtgta gagccagaac gtaccggcag cgccaatgct atcaagcagt
    53041 gtcaggaagg ccgcgccgat aatcatattc gacacccagt ttgtcgtcgt cgaacaggta
    53101 ataccgaaat cgcggcattt cagcggctga atttcagagc acaggatcca caccactggc
    53161 gcggcgctca tcgcataacc ggcaatacac atcatcgtca tgccaacaga gagccaggac
    53221 aagccactgg aagccgtacc gttatcaaac tgcatcaggc aatagcccag caccagagtg
    53281 cctaacgcca tcacgctgaa accaattttc agagccggtt tacgacctgc tttatctacc
    53341 gtaaagaccg caatgaaggt cgcgaacata aaggtcagtc ccacgaccag agtcgcaatc
    53401 atctgttgtt ctgtggtcgt aaagcccgcc attttgaaga tacgcggcgc gtagtacatg
    53461 atgatgttca taccggtaaa ctgctgcatc gcctgcaaca acataccgag aaacacagca
    53521 cgacggacgt tacggttgat cttaaacagt gcccaaccgc cctgttttaa cttcaggctt
    53581 tcacgaattt cgttgagttc ttctcgcgct ttttccgacg tatcgcgcag catacgcaac
    53641 acttcttccg cctcaatatg acgccccttt tccgccagcc agcgcgggct atttggcagg
    53701 aagaccacca gaataatcag cagaactgct ggtaaagcaa gaacccccaa cattgcgcgc
    53761 cagttaccgc tataactgaa cgctgtatcg gataaaaacg ccagcacgat gccgagtgtg
    53821 accatcaact ggtacatact gatcatctta ccgcgaacgt tttcacttgc catttcagaa
    53881 agatacagag gagcggtgta agacgcgatc ccgacagcaa tgcccagcac cacacgagcg
    53941 gcgattaaca tctctacgct ggtcgcaaaa gcggacccta tagaaccgag tacaaacagg
    54001 atggcccccg ccatcaggct gtatttacgc cccaggcgga acgacagcca accattaaac
    54061 agcgcaccaa ttgctgcgcc gagcatcatg ctactaacca cccattcctg caaacgactg
    54121 gtcagcacaa agtgatcggt aatgaacggc aacgctccgg cgattacgcc gatatcaaga
    54181 ccaaataaca atcctgcgac cgcagcagct accgaaacaa acatattcat acgccgcgta
    54241 tcacgcaaag aacgtggcgt taaagcagat tccgtattga tagtaaccat tttttcctgc
    54301 cagcagagag taagacattg tgaaaaaata cgtgaacaat tcacacaggt gtcaggtcgg
    54361 aaacagcata aatatggatt aaattgctgc g
//
LOCUS       DAZSIO010000007        53080 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZSIO010000007 DAZSIO010000000
VERSION     DAZSIO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.007"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            290..376
                     /gene="leuL"
                     /locus_tag="ACHX5T_000355"
     CDS             290..376
                     /gene="leuL"
                     /locus_tag="ACHX5T_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR8465699.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            469..2040
                     /gene="leuA"
                     /locus_tag="ACHX5T_000356"
     CDS             469..2040
                     /gene="leuA"
                     /locus_tag="ACHX5T_000356"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR8465700.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            2040..3131
                     /gene="leuB"
                     /locus_tag="ACHX5T_000357"
     CDS             2040..3131
                     /gene="leuB"
                     /locus_tag="ACHX5T_000357"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR8465701.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3134..4534
                     /gene="leuC"
                     /locus_tag="ACHX5T_000358"
     CDS             3134..4534
                     /gene="leuC"
                     /locus_tag="ACHX5T_000358"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR8465702.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4545..5150
                     /gene="leuD"
                     /locus_tag="ACHX5T_000359"
     CDS             4545..5150
                     /gene="leuD"
                     /locus_tag="ACHX5T_000359"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR8465703.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(5199..6377)
                     /gene="setA"
                     /locus_tag="ACHX5T_000360"
     CDS             complement(5199..6377)
                     /gene="setA"
                     /locus_tag="ACHX5T_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR8465704.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(6479..6610)
                     /gene="sgrT"
                     /locus_tag="ACHX5T_000361"
     CDS             complement(6479..6610)
                     /gene="sgrT"
                     /locus_tag="ACHX5T_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR8465705.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6699..8354
                     /gene="sgrR"
                     /locus_tag="ACHX5T_000362"
     CDS             6699..8354
                     /gene="sgrR"
                     /locus_tag="ACHX5T_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR8465706.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8388..8487
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8518..9501
                     /gene="thiB"
                     /locus_tag="ACHX5T_000363"
     CDS             8518..9501
                     /gene="thiB"
                     /locus_tag="ACHX5T_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR8465707.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9477..11087
                     /gene="thiP"
                     /locus_tag="ACHX5T_000364"
     CDS             9477..11087
                     /gene="thiP"
                     /locus_tag="ACHX5T_000364"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR8465708.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11071..11769
                     /gene="thiQ"
                     /locus_tag="ACHX5T_000365"
     CDS             11071..11769
                     /gene="thiQ"
                     /locus_tag="ACHX5T_000365"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR8465709.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(11883..12647)
                     /locus_tag="ACHX5T_000366"
     CDS             complement(11883..12647)
                     /locus_tag="ACHX5T_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR8465710.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12733..13611)
                     /gene="araC"
                     /locus_tag="ACHX5T_000367"
     CDS             complement(12733..13611)
                     /gene="araC"
                     /locus_tag="ACHX5T_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR8465711.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13950..15650
                     /gene="araB"
                     /locus_tag="ACHX5T_000368"
     CDS             13950..15650
                     /gene="araB"
                     /locus_tag="ACHX5T_000368"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR8465712.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15661..17163
                     /gene="araA"
                     /locus_tag="ACHX5T_000369"
     CDS             15661..17163
                     /gene="araA"
                     /locus_tag="ACHX5T_000369"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR8465713.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17363..18058
                     /gene="araD"
                     /locus_tag="ACHX5T_000370"
     CDS             17363..18058
                     /gene="araD"
                     /locus_tag="ACHX5T_000370"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR8465714.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLHKH
                     YLRKHGAKAYYGQ"
     gene            18133..20484
                     /gene="polB"
                     /locus_tag="ACHX5T_000371"
     CDS             18133..20484
                     /gene="polB"
                     /locus_tag="ACHX5T_000371"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR8465715.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20649..23555
                     /gene="rapA"
                     /locus_tag="ACHX5T_000372"
     CDS             20649..23555
                     /gene="rapA"
                     /locus_tag="ACHX5T_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR8465716.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23567..24226
                     /gene="rluA"
                     /locus_tag="ACHX5T_000373"
     CDS             23567..24226
                     /gene="rluA"
                     /locus_tag="ACHX5T_000373"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR8465717.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24343..25158)
                     /gene="djlA"
                     /locus_tag="ACHX5T_000374"
     CDS             complement(24343..25158)
                     /gene="djlA"
                     /locus_tag="ACHX5T_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR8465718.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25413..27767
                     /gene="lptD"
                     /locus_tag="ACHX5T_000375"
     CDS             25413..27767
                     /gene="lptD"
                     /locus_tag="ACHX5T_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR8465719.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27820..29106
                     /gene="surA"
                     /locus_tag="ACHX5T_000376"
     CDS             27820..29106
                     /gene="surA"
                     /locus_tag="ACHX5T_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR8465720.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29106..30095
                     /gene="pdxA"
                     /locus_tag="ACHX5T_000377"
     CDS             29106..30095
                     /gene="pdxA"
                     /locus_tag="ACHX5T_000377"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR8465721.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30092..30913
                     /gene="rsmA"
                     /locus_tag="ACHX5T_000378"
     CDS             30092..30913
                     /gene="rsmA"
                     /locus_tag="ACHX5T_000378"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR8465722.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30916..31293
                     /gene="apaG"
                     /locus_tag="ACHX5T_000379"
     CDS             30916..31293
                     /gene="apaG"
                     /locus_tag="ACHX5T_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR8465723.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31300..32142
                     /gene="apaH"
                     /locus_tag="ACHX5T_000380"
     CDS             31300..32142
                     /gene="apaH"
                     /locus_tag="ACHX5T_000380"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR8465724.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32220..32699)
                     /gene="folA"
                     /locus_tag="ACHX5T_000381"
     CDS             complement(32220..32699)
                     /gene="folA"
                     /locus_tag="ACHX5T_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR8465725.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32890..34752)
                     /gene="kefC"
                     /locus_tag="ACHX5T_000382"
     CDS             complement(32890..34752)
                     /gene="kefC"
                     /locus_tag="ACHX5T_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR8465726.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWVKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34745..35275)
                     /gene="kefF"
                     /locus_tag="ACHX5T_000383"
     CDS             complement(34745..35275)
                     /gene="kefF"
                     /locus_tag="ACHX5T_000383"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR8465727.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35323..36717)
                     /locus_tag="ACHX5T_000384"
     CDS             complement(35323..36717)
                     /locus_tag="ACHX5T_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8465728.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(36776..37063)
                     /gene="fixX"
                     /locus_tag="ACHX5T_000385"
     CDS             complement(36776..37063)
                     /gene="fixX"
                     /locus_tag="ACHX5T_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR8465729.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37060..38346)
                     /locus_tag="ACHX5T_000386"
     CDS             complement(37060..38346)
                     /locus_tag="ACHX5T_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR8465730.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38397..39338)
                     /locus_tag="ACHX5T_000387"
                     /pseudo
     CDS             complement(38397..39338)
                     /locus_tag="ACHX5T_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(39353..40123)
                     /locus_tag="ACHX5T_000388"
     CDS             complement(39353..40123)
                     /locus_tag="ACHX5T_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR8465731.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            40479..41993
                     /gene="caiT"
                     /locus_tag="ACHX5T_000389"
     CDS             40479..41993
                     /gene="caiT"
                     /locus_tag="ACHX5T_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFR8465732.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            42024..43166
                     /gene="caiA"
                     /locus_tag="ACHX5T_000390"
     CDS             42024..43166
                     /gene="caiA"
                     /locus_tag="ACHX5T_000390"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR8465733.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            43295..44512
                     /gene="caiB"
                     /locus_tag="ACHX5T_000391"
     CDS             43295..44512
                     /gene="caiB"
                     /locus_tag="ACHX5T_000391"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFR8465734.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            44586..46139
                     /gene="caiC"
                     /locus_tag="ACHX5T_000392"
     CDS             44586..46139
                     /gene="caiC"
                     /locus_tag="ACHX5T_000392"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR8465735.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            46248..47033
                     /gene="caiD"
                     /locus_tag="ACHX5T_000393"
     CDS             46248..47033
                     /gene="caiD"
                     /locus_tag="ACHX5T_000393"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR8465736.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            47039..47629
                     /gene="caiE"
                     /locus_tag="ACHX5T_000394"
     CDS             47039..47629
                     /gene="caiE"
                     /locus_tag="ACHX5T_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR8465737.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(47715..48110)
                     /gene="caiF"
                     /locus_tag="ACHX5T_000395"
     CDS             complement(47715..48110)
                     /gene="caiF"
                     /locus_tag="ACHX5T_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR8465738.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(48372..51593)
                     /gene="carB"
                     /locus_tag="ACHX5T_000396"
     CDS             complement(48372..51593)
                     /gene="carB"
                     /locus_tag="ACHX5T_000396"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR8465739.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(51611..52759)
                     /gene="carA"
                     /locus_tag="ACHX5T_000397"
     CDS             complement(51611..52759)
                     /gene="carA"
                     /locus_tag="ACHX5T_000397"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR8465740.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
BASE COUNT        12740 a        14438 c        13928 g        11974 t
ORIGIN      
        1 catataattc aatacaaatc atcaggatag gttttgcaac gcgtgcattt tgtccccttt
       61 ttcctcgttg attagatgca aaaatttatg ctgaaatatg tcaaccgatg aaaagcgtcg
      121 gtagttaagc agaaattaat atcgcttact ttaaccaccg tagcacaatt agctaatttt
      181 acggatgcag aactcacgct ggcgggacgt ttttattgcg tcagggttga catccgtttt
      241 tgtatccagt aactctaaaa gcatatcgca ttcatctgga gctgatttaa tgactcacat
      301 cgttcgcttt atcggtctac tactactaaa cgcatcttct ttgcgcggta gacgagtgag
      361 cggcatccag cattaagcca gcacgcagtc aaacaaaaaa cccgcgccat tgcgcgggtt
      421 tttttatgcc cgaagcgagg cgctctaaaa gagacaagga cccaaaccat gagccagcaa
      481 gtcattattt tcgataccac attgcgcgac ggtgaacagg cgttacaggc aagcttgagt
      541 gtgaaagaaa aactgcaaat tgcgctggcc cttgagcgta tgggtgttga cgtgatggaa
      601 gtcggtttcc ccgtctcttc gccgggcgat tttgaatcgg tgcaaaccat cgcccgccag
      661 gttaaaaaca gccgcgtatg tgcgttagct cgctgcgtgg aaaaagatat cgacgtggcg
      721 gccgaatccc tgaaagtcgc cgaagccttc cgtattcata cctttattgc tacctcccca
      781 atgcacatcg ccaccaagct gcgcagcacc ctagacgagg tgatcgaacg cgctatctat
      841 atggtgaaac gcgcccgtaa ttacaccgat gatgttgaat tttcttgcga agatgccggg
      901 cgtacaccca ttgccgatct ggcgcgagtg gtcgaagcgg cgattaatgc cggtgccacc
      961 accatcaaca ttccggacac cgtgggctac accatgccgt ttgagttcgc cggaatcatc
     1021 agcggcctgt atgaacgcgt acctaacatc gacaaagcca ttatttccgt acatacccac
     1081 gacgatttag gcctggcggt cggcaactca ctggcggcgg tacatgccgg tgcacgccag
     1141 gtggaaggtg caatgaacgg gatcggcgag cgtgccggta actgttcgct ggaagaagtc
     1201 atcatggcga ttaaagttcg taaggatatt ctcaacgtcc acaccgccat taatcaccaa
     1261 gagatatggc gcaccagcca gttagttagc cagatttgta atatgccgat cccggcaaac
     1321 aaagccattg ttggcagcgg cgcattcgct cactcctccg gtatccacca ggatggcgtg
     1381 ctgaaaaacc gcgaaaacta cgaaatcatg acaccagaat ctattggtct gaaccaaatc
     1441 cagctgaatc tgacctctcg ttcggggcgt gcggcggtga aacatcgcat ggatgagatg
     1501 gggtataaag aaagtgaata taatttagac aatttgtacg atgctttcct gaagctggcg
     1561 gacaaaaaag gtcaggtgtt tgattacgat ctggaggcgc tggccttcat cggtaagcag
     1621 caagaagagc cggagcattt ccgtctggat tacttcagcg tgcagtctgg ctctaacgat
     1681 atcgccaccg ccgccgtcaa actggcctgt ggcgaagaag tcaaagcaga agccgccaac
     1741 ggtaacggtc cggtcgatgc cgtctatcag gcaattaacc gcatcactga ctataacgtc
     1801 gaactggtga aatacagcct gaccgccaaa ggtcacggta aagatgcgct gggtcaggtg
     1861 gatattgtca ccaactacaa cggtcgccgc ttccacggcg tcggcctggc caccgatatt
     1921 gtcgagtcct ccgccaaagc catggtgcac gtactgaaca atatctggcg tgccgcagaa
     1981 gtcgaaaaag agttgcaacg caaaactcaa cacaacgaaa acaacaagga aaccgtgtga
     2041 tgtcgaagaa ttaccatatt gccgtattgc cgggagacgg tattggtccg gaagtgatga
     2101 cccaggcgct gaaagtgctg aatgccgtgc gcaaccgctt tgcgatgcgc atcaccacca
     2161 gccattacga tgtaggcggc gcagccattg ataaccacgg gcaaccactg ccgcctgcga
     2221 cggttgaagg ttgtgagcaa gccgatgccg tgctgtttgg ctcggtaggc ggcccgaagt
     2281 gggaacattt accaccagac cagcaaccag aacgcggcgc gctgctgccg ctgcgtaagc
     2341 acttcaaatt attcagcaac ctgcgcccgg caaaactgta tcaggggctg gaagcattct
     2401 gtccgctgcg tgcagacatt gccgctaacg gcttcgacat ccagtgcgtg cgcgaactga
     2461 ccggcggcat ctatttcggt cagccaaaag gccgcgaagg tagcggacaa tatgaaaaag
     2521 cgtttgatac cgaggtgtat caccgttttg agatcgagcg tattgcccgc atcgcgtttg
     2581 aatctgctcg taaacgtcgc cacaaagtca cctcaatcga caaagctaac gtgctgcaat
     2641 cctctatttt atggcgtgaa atcgtcaacg agatcgccac ggaatacccg gatgtcgaac
     2701 tggcgcatat gtacatcgac aacgccacca tgcagctgat taaagatcca tcacagtttg
     2761 acgttctgct gtgctccaac ctgtttggcg acattctgtc tgacgagtgc gcaatgatca
     2821 ctggctcgat gggcatgttg ccttccgcca gcctgaacga gcaaggtttt ggactgtatg
     2881 aaccggcggg cggctcggca ccagatatcg caggcaaaaa catcgccaac ccgattgcac
     2941 aaatcctgtc gctggcactg ctgctgcgtt acagcctgga tgccgatgat gcggcttccg
     3001 ccattgaacg cgccattaac cgtgcattag aagaaggcat tcgcaccggg gatttagccc
     3061 gtggcgctgc cgccgttagt accgatgaaa tgggcgatat cattgcccgc tatgtggcag
     3121 aaggggtgta atcatggcta aaacgttata cgaaaaatta ttcgacgctc acgtagtgta
     3181 cgaagccgaa aacgaaaccc cgctgttata tatcgaccgc cacctggtgc atgaagtgac
     3241 ctcaccgcag gcgttcgatg gtctgcgcgc ccacggtcgc ccggtacgtc agccgggcaa
     3301 aaccttcgcc actatggatc acaacgtctc tacccagacc aaagacatta atgcctgtgg
     3361 tgaaatggcg cgcatccaga tgcaggagct gattaaaaac tgcaaagaat ttggcgtcga
     3421 gctgtatgac ctgaatcacc cgtatcaggg gatcgtccac gtaatggggc cggaacaggg
     3481 cgtcaccttg ccggggatga ccattgtctg cggcgactcg cataccgcca cccacggcgc
     3541 gtttggcgca ctggcctttg gtatcggcac ttccgaagtt gaacacgtac tggcaacaca
     3601 aaccctgaaa cagggccgtg cgaagaccat gaaaattgaa gtccagggca aagccgcgcc
     3661 gggcattacc gcaaaagata tcgtgctggc aattatcggt aaaactggca gcgcaggcgg
     3721 caccgggcat gtggtggagt tttgcggcga agcaattcgt gatttaagca tggaaggtcg
     3781 tatgaccctg tgcaatatgg cgatcgaaat gggcgcaaaa gccgctctgg ttgctcctga
     3841 cgaaaccacc tttaactatg tcaaaggccg tctgcatgcg ccgaaaggca aagatttcga
     3901 cgacgccgtt gcctactgga aaaccctgca aaccgacgaa ggcgcaactt tcgataccgt
     3961 tgtcactctg caagcagaag aaatttcacc gcaggtcacc tggggcacca atcccggcca
     4021 ggtgatttct gtgaacgaca atattcccga tccggcttcg tttgccgatc cggttgaacg
     4081 tgcgtcggca gaaaaagcgc tggcctatat ggggctgaaa ccgggtattc cgctgaccga
     4141 agtggctatc gacaaagtgt ttatcggttc ctgtaccaac tctcgcattg aagatttacg
     4201 cgcggcagcg gagatcgcca aagggcgaaa agtcgcgcca ggcgtgcagg cactggtggt
     4261 tcccggctct ggcccggtaa aagcccaggc ggaagcggaa ggtctggata aaatatttat
     4321 tgaagccggt tttgaatggc gcttgcctgg ctgctcaatg tgtctggcga tgaacaacga
     4381 ccgtctgaat ccgggcgaac gttgtgcatc caccagcaac cgtaactttg aaggccgcca
     4441 ggggcgcggc gggcgcacgc atctggtcag cccggcaatg gctgccgctg ctgctgtgac
     4501 cggacatttc gccgacattc gcaacattaa ataaggagca caccatggca gagaaattta
     4561 tcaaacacac aggcctggtg gttccgctgg atgccgccaa tgtcgatacc gatgcaatca
     4621 tcccgaaaca gtttttgcag aaagtgaccc gtacgggttt tggcgcgcat ctgtttaacg
     4681 actggcgttt tctcgatgaa aaaggccaac agccaaaccc ggacttcgtg ctgaacttcc
     4741 cgcagtatca gggcgcttcc attttgctgg cacgagaaaa cttcggctgt ggctcctcgc
     4801 gtgagcacgc gccctgggca ttgaccgact acggttttaa agtggtgatt gcgccgagtt
     4861 ttgctgacat cttctacggc aatagcttta acaaccagct gctgccggtg aaattaagcg
     4921 atgcagaagt ggacgaactg tttgcgctgg tgaaagccaa tccggggatc catttcgacg
     4981 tggatctgga agcgcaagag gtgaaagcgg gagagaaaac ctatcgcttt accatcgatg
     5041 ccttccgccg ccactgcatg atgaatggtc tggacagcat cggcctgacc ctgcagcacg
     5101 acgacgctat tgcgtcttat gaagcaaaac aacctgcgtt tatgaattag accccttgcc
     5161 cggtcaaatg accgggcttt ccgctatcgt ccacgtcatc aaacgtcttt aacctttgcg
     5221 gttaaaaata atgcgacaac agaaataatc gcaattaccc agtagacagc aaagtgcccc
     5281 caactttgtg caattgctcc ctgaataaca ccagccagaa ttaccccggt agagatagtg
     5341 ttggtaaata aggtggtagc tgcccccgct cttccaggca ttaaatcctg aaaccatagc
     5401 atcccaatac ccgcaacaat gccgataaat acagcgttaa aaagttgcag cgtcatcaac
     5461 gccatacggc tatgaaagaa aatcaatcca gtgtaaaaca gcactcctgc caccactgca
     5521 atgaccatca ttcgccgctt accataacgt ttgacatagt agccagccag aatcattgct
     5581 ggtatttcca gtccagctgc cgtccccatc aggaaacccg ccagtttgtc tggcaatcct
     5641 aaatcgctac tgatccacaa tggcatatca ataatgtaca tagtgttgca ggtccacatt
     5701 aacgtcgagg cgacaaataa catccgtacg ttactatcct gccagtcacc ttgcattgat
     5761 aaagcatttt ccgacggcag ttctacccgc gccacagaca gaagcataaa tgcaatcaat
     5821 accagactga gtgtgaatat cccggcggca atcgaaaaca tcactgtaaa gccgtaattc
     5881 aacgccagca taaaggccag cggtggaccg attacccatg ccagagaaag ctgcgcacgc
     5941 atcaccgagc taaacatcac cacttctcgc gccgagttat ccgcgtattc ccgcgccaga
     6001 gcaaataact gtggcattgc cgtattggcc agagatgcca gaagcacacc acaggtgata
     6061 agcgtcagat aatgacgatt aaatgcaaac agtagcgcat tgccaatagc catcaaacag
     6121 caaaatataa tcagttttcg ccgatcgccc tgactgtcag aacgttttgc caaccagagg
     6181 cttacgccga tcccagcaat agcattcacc gtataaaaga gtccgatcca gaaaggttgc
     6241 gcgccaacct cacgactcag aaataagctc aatgtaggag cctgtagcgc cccggccacc
     6301 cccatcataa aagcgaccag cataaatgcc gcgtaaacac cgttcatacg gcgagccatc
     6361 gtcattatcc agatcatacg ttcccttttt agcgcggcga gaataaaaaa aaccagcagg
     6421 tataatctgc tggcgggtga ttttacacca atactcagtc acacatgatg caggcaagtc
     6481 aactttcaga attgcggtca tcccactgca tcagtccttc aagcatggtt aatcgttgtg
     6541 ggacgcttaa ccaacgcaac cagcacaact tcgctgtcgc ggtaaaatag tgctgataaa
     6601 actgacgcat ggggcacctc cttgcttcat cgttaggaat tattgactta atatagggaa
     6661 aataaaattg ctgtcttttg cgcaggagtt ccccttttat gccatctgct cgtctgcaac
     6721 aacagttcat ccgcctgtgg caatgctgcg agggtaaatc gcaggacaca acgctcaacg
     6781 aactggcagc gttattgagc tgctcgcgtc gtcatatgcg taccctgctc aacaccatgc
     6841 aggatcgcgg ctggctgacg tgggaagcgg aagtcgggcg cggtaaacgc tcgcgtctga
     6901 cattcctcta taccgggctg gcgcttcagc aacagcgggc ggaagacctg ctggagcagg
     6961 atcgtatcga ccaactagtg cagttggttg gcgacaaagc gactgtgcgg caaatgctgg
     7021 tttctcatct gggccgcagc ttccgccagg ggcggcacat cctgcgcgtg ctctactatc
     7081 gtccgttgcg taatctgcta cctggcagcg cattgcgccg ttccgaaacc catattgccc
     7141 ggcaaatctt cagttcgcta acgcgcataa atgaggaaaa tggggaactg gaagcagaca
     7201 tcgcccacca ctggcagcaa atatcaccgc ttcactggcg tttctttttg cgtccaggag
     7261 tccattttca tcatggtcgt gaactggaaa tggacgacgt gatcgcctct ttaaaacgaa
     7321 tcaatacgct gccgctctat tcgcatatta cggacattgt gtcgccgacg ccctggacgc
     7381 tggatatcca tctcacgcag ccggaccgct ggttgccgtt actgctgggg caagttccgg
     7441 cgatgatcct gccgtgcgaa tgggaaaccc tcagtaactt tgccagccat cctattggca
     7501 ccggtccgta tgcggtgatt cgcaacagca ccaatcaact gaaaattcag gcattcgatg
     7561 acttcttcgg ttaccgggca ttaatcgacg aagttaacgt ctgggttctg ccggaaattg
     7621 ccgacgagcc agccggtggt ctgatgctaa aaggtccaca gggcgagaaa aaagagattg
     7681 aaagccgcct ggaggaaggt tgctactatt tactgttcga cagccgcacc catcgcgggg
     7741 cgaatcagca agtcagggac tgggtaagct atgtgctttc tccaactaat ctggtctatt
     7801 tcgctgagga acagtaccag caactgtggt tcccggctta tggactgctc ccccgttggc
     7861 accatgcccg caccataaag agcgaaaaac cggctggcct ggaaagcctc accctgacct
     7921 tttatcagga tcacagtgag catcgggtga ttgccgggat catgcagcag attctggcaa
     7981 gtcaccaggt cacgctggaa atcaaagaga tcagctacga tcagtggcat gaaggagaga
     8041 tcgagagcga tatctggctt aatagcgcca actttacgct gccgctggat ttttcgctgt
     8101 tcgcgcactt gtgcgaggtg ccgctgctcc aacactgtat tccgatcgac tggcaagccg
     8161 acgccgcccg ctggcgcaat ggcgaaatga acctggcgaa ctggtgccag caactggtcg
     8221 ccagtaaagc aatggtgccg cttatccacc actggctgat cattcaggga caacgcagta
     8281 tgcgcggcct gcgtatgaat accctcggct ggttcgattt taaatcagcg tggtttgcgc
     8341 caccggatcc atgattgctg gtagataaca aaatcactac actaacgccg ttctcaacgg
     8401 ggtgccacgc aaacgcgtgc gctgagaaaa tacccgtcga acctgatccg gataacgccg
     8461 gcgaagggat ttgaggctcc ttctcaagtc ctttgccact cttttttgag gtgcaaagtg
     8521 ttaaaaaaat gtcttcccct gctgttgctg tgcacagcgc ccgttttcgc taaacccgtt
     8581 ctgactgttt atacctacga ttccttcgcc gccgactggg ggcctggtcc aaagatcaaa
     8641 aaagcttttg aagccaactg taactgcgaa ctgaaactgg tggcgctgga agatggcgtt
     8701 tcgcttctca accgtctacg gatggaaggc aaaaatagta aagccgatgt ggtgctgggg
     8761 ctggataaca atctgttaga cgccgccagt aaaaccggac tatttgccaa aagcggtgtg
     8821 gcagcggatg ccgttaacgt tcccggcggc tggaataatg acactttcgt cccgtttgat
     8881 tacggctatt tcgccttcgt ctatgacaag aacaaactga aaaacccgcc gcaaagcctg
     8941 aaagaattgg ttgagagcga tcagaactgg cgggtgattt atcaggatcc gcgtaccagc
     9001 acgccggggc tgggtctgct gttgtggatg caaaaagtct atggcgatga cgccccacaa
     9061 gcctggcaaa aactggcgaa gaaaaccgtc acggtcacca aaggctggag cgaagcctac
     9121 ggcctgtttt taaaaggtga aagcgatctg gtactgagtt acaccacctc tccggcttat
     9181 cacattctcg aagagaaaaa agataactac gccgccgcga actttagcga aggtcactat
     9241 ctgcaggtgg aagtcgccgc ccgcaccgct gccagcaagc agccggagct ggcgcaaaaa
     9301 ttcctccagt ttatggtttc tccggctttt cagaatgcga tcccaaccgg taactggatg
     9361 tatccggtgg caaatgtcac gctgcctgcc gggtttgaac aattgaccaa accggccacc
     9421 acgctggagt tcacaccagc tgaagtggcg gcacaacgtc aggcatggat tagcgaatgg
     9481 caacgcgccg tcagccgtta attcccggct ggttaattcc aggtgtaagc gccgccacgc
     9541 tggtggtagc ggttgcgctc gcggcgtttc tcgccctgtg gtggaacgcg ccgcagggta
     9601 actgggtggc agtctggcag gacagctacc tgtggcatgt ggtgcgcttc tccttctggc
     9661 aggcgtttct ctcggcgcta ctctctgtcg tacccgcgat attcctcgcc cgcgccctct
     9721 atcgcaggcg ctttccgggt cggctggcgc tgttgcgtct gtgcgcaatg accttgatcc
     9781 tcccggtgct agttgctgtt ttcggcattc ttagcgtcta tggtcgtcag ggctggctgg
     9841 catcgctctg ccaatcgctc ggtctggagt ggaccttttc gccctacggc ctgcaaggca
     9901 ttttgctggc gcacgtattt tttaatctgc cgatggcgag ccgcttatta ctccaggcac
     9961 tggaaaacat tcccggcgaa caacgtcagc ttgccgccca gcttgggatg cgcggctggc
    10021 attttttccg cttcgtcgaa tggccgtggc tacggcgaca aatcccgccg gttgccgcgc
    10081 ttatctttat gctctgtttc gccagcttcg ccaccgtgct atcgctgggc ggcggtccgc
    10141 aggcaaccac tatcgagctg gctatttatc aggcgctgag ttacgactac gatcctgccc
    10201 gcgcggcgat gctggcgctg ctccagatgg tgtgttgcct tgggctggtg ctgttgagtc
    10261 agcgactaag taaggccatt gcgcccggca ccacgctgct gcaaggctgg cgcgatccgg
    10321 acgatcgtct gcatagccgc atttgcgaca cggtgttaat agtgctggcg ctgctgctgt
    10381 tgctgccacc gttgctggcg gtgatcgtcg atggggtaaa tcgccagttg ccggaagtgc
    10441 tggcacaacc ggtgctgtgg caggcgctgt ggacctcgtt gcgtattgcg ctggcggcag
    10501 gtgtattgtg cgtagtgctg accatgatgc tgctatggag cagtcgcgaa ctgcgggcgc
    10561 ggcagaaaat gctggcgggt caggcgctgg agatgagcgg catgttgatc ctcgccatgc
    10621 cggggattgt gctggctacc ggcttctttt tactgctcaa caacactatc ggcctgccac
    10681 aatctgctga cggcattgtg attttcacca atgcgttaat ggcgatccct tatgcgctga
    10741 aagtactgga aaacccgatg cgcgatatca ccgcccgcta cagtatgttg tgtcagtcgc
    10801 tgggcattga aggctggtcg cgcttaaaag tggtcgagct gcgcgccctg aaacgtccac
    10861 tggcgcaggc gctggctttt gcctgcgtgc tgtcgattgg tgattttggc gtagtggcgt
    10921 tgttcggtaa cgatgatttc cgtaccctgc cgttttatct ctaccagcaa attggctcct
    10981 atcgcagcca ggacggcgcg gtcaccgcgt taattctgct actactctgt tttctgctgt
    11041 ttaccgtgat tgaaaaacta ccggggcgaa atgttaaaac tgactgatat cacctggctt
    11101 tatcaccatt tgccgatgcg ttttagctta acggtagaac gcggcgagca ggtggcaatc
    11161 ctcgggccaa gcggcgcggg taaaagtacc ctgctgaatt tgattgccgg ttttctgacg
    11221 ccagccagcg gttcgctgac tatcgatggt gttgatcaca ccacgacgcc gccgtcacgc
    11281 cgtccggtgt cgatgctgtt tcaggagaac aacctgttca gccatctgac ggtcgcgcag
    11341 aacatcgggc tggggctaaa tccggggttg aaactgaacg cggcacagca ggagaaaatg
    11401 cacgcgattg cccgtcagat ggggattgat aatttaatgg cgcggttacc gggcgagctt
    11461 tccggcggtc agcgacagcg cgtggcgtta gcgcgttgtc tggtgcgcga acagccgatt
    11521 ttattgctcg atgaaccgtt ctctgcgctc gatccggcgt tacgtcagga gatgctgacg
    11581 ctggtgagca cgagctgcca gcagcaaaaa atgacgctat tgatggtgtc gcacagtgtg
    11641 gaagatgcgg cgcggatcgc cacacgctcg gtggtggtcg ccgacgggcg catcgcctgg
    11701 cagggtaaga ctgaagaatt gttgagcggt aaggcgagtg cttcggcaat attggggatt
    11761 acgggttagt atgcactgtc agatgcggcg tgaacacctt atccgaccta cggacagtgc
    11821 gttttgtagg cctgataaga cgcggtcagc gtcgcatgag gcaacccgca caagacaatc
    11881 ccctaaaccc caaccacttt acgcaaaata tcgatataca ccggcatcaa cgggtggcga
    11941 attaacacca ccagcgccac cacgccgatg gcagaaatca acggcgtcag ccacaacaaa
    12001 cggccgcgcg acaaataatg acttaaacga tcggttgctt taccgctgcg ccataaccgc
    12061 cagcacagcc agccaccaac ccacaaaaat accgccgtcg ccagcagcaa ccatttaaac
    12121 tcgccgctct gcattccggc tggaatatcg atcgccgcgc ccgccagaat cccaggcagg
    12181 aagtaaaacg gtggccacag caggcagccg ataatattcg gcgtaatgaa ttttgccacc
    12241 ggcagatcca gcattcccgc caccattggc accagcggac gcgtcgggcc aacaaagcga
    12301 ccgaccagaa tggtgaacat gctgtgttga tgcaacgcat gttcagtttt atcgagtaat
    12361 gctttgtttt tcttcagaaa tgaccagcga tgcaacggct ttttaaaacg ccagcccagc
    12421 cagaaggaga tccagtcgcc cagcagacaa ccgacaatcc ctgccagcca agcgtgccag
    12481 aaacttaact cgccgctgcc aatcagcgct cccagccccg ccatcagcac cgtaccgggt
    12541 agaatcaaac cgaccagcgc cagcgactcc agaaaggcca ccaacaccac cgccatcaat
    12601 gaatacacgg tagattgggt aataaagtgt tccagcaatg cttgcataat gtgcctgtca
    12661 aatggacgaa gcagggattc tgcaaaccct atgctactcc gtcaagccgt caattgtctg
    12721 attcgttacc aattatgaca acttgacggc tacatcattc actttttctt cacaaccggc
    12781 acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga aatagagttg
    12841 atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag
    12901 cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg
    12961 ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc
    13021 gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacacg
    13081 attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg
    13141 ctcaagcaga ttcatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat
    13201 gatttgccca aacaggtcgc tgaaatacgg ctggtgcgct tcatccgggc gaaagaaacc
    13261 cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa
    13321 gtaaacccac tggtgatacc attcgcgagc ctccggatga caaccgtagt gatgaatctc
    13381 tcctggcggg aacagcaaaa tatcacccgg tcggcagaca aattctcgtc cctgattttt
    13441 caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg
    13501 gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg ccaccagatg
    13561 ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca tacttttcat
    13621 actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt gccgtcactg
    13681 cgtcttttac tggctcttct cgctaaccca accggtaacc ccgcttatta aaagcattct
    13741 gtaacaaagc aggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg
    13801 cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt
    13861 atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc
    13921 atacccgttt ttttggatgg agtgaaacga tggcgattgc aattggcctc gattttggca
    13981 gtgattctgt gcgagctttg gcggtggact gcgccagcgg tgaagagatc gccaccagcg
    14041 tagagtggta tccccgttgg caaaaagggc aattttgtga tgccccgaat aaccagttcc
    14101 gtcatcatcc gcgtgactac attgagtcaa tggaagcggc actgaaaacc gtgcttgcag
    14161 agcttagcgt cgaacagcgc gcagctgtgg tcgggattgg cgttgacagt accggctcga
    14221 cgcccgcacc gattgatgcc gacggtaacg tgctggcgct gcgcccggag tttgccgaaa
    14281 acccgaacgc gatgttcgta ttgtggaaag accacactgc ggttgaagaa gcggaagaga
    14341 ttacccgttt gtgccacgcg ccgggcaacg ttgactactc ccgctatatt ggcggtattt
    14401 attccagcga atggttctgg gcaaaaatcc tgcatgtgac tcgccaggac agcgccgtgg
    14461 cgcaatctgc cgcatcgtgg attgagctgt gcgactgggt gccagctctg ctttccggta
    14521 ccacccgccc gcaggatatt cgtcgcggac gttgcagcgc cgggcataaa tctctgtggc
    14581 acgaaagctg gggcggcttg ccgccagcca gtttctttga tgagctggac ccgatcctca
    14641 atcgccattt gccttccccg ctgttcactg acacctggac tgccgatatt ccggtgggca
    14701 ccttatgccc ggaatgggcg cagcgtctcg gcctgcctga aagcgtggtg atttccggcg
    14761 gcgcgtttga ctgccatatg ggcgcagttg gcgcaggcgc acagcctaac gcactggtaa
    14821 aagttatcgg tacttccacc tgcgacattc tgattgccga caaacagagc gttggcgagc
    14881 gggcagttaa aggtatttgc ggtcaggttg atggcagcgt ggtgcctgga tttatcggtc
    14941 tggaagcagg ccaatcggcg tttggtgata tctacgcctg gtttggtcgc gtactcggct
    15001 ggccgctgga acagcttgcc gcccagcatc cggaactgaa agcgcaaatc aacgccagcc
    15061 agaaacaact gcttccggcg ctgaccgaag catgggccaa aaatccgtct ctggatcacc
    15121 tgccggtggt gctcgactgg tttaacggtc gtcgcacgcc aaacgctaac caacgcctga
    15181 aaggggtgat taccgatctt aacctcgcta ccgacactcc gctgctgttc ggcggtttga
    15241 ttgctgccac cgcctttggc gcacgcgcaa tcatggagtg ctttaccgat caggggatcg
    15301 ccgtcaataa cgtgatggcg ctgggcggca tcgcgcggaa aaaccaggtc attatgcagg
    15361 cctgctgcga cgtgctgaat cgcccgctgc aaattgttgc ctctgaccag tgctgtgcgc
    15421 tcggtgcggc gatttttgct gccgtcgccg cgaaagtgca cgcagacatc ccatcagccc
    15481 agcaaaaaat ggccagtgcg gtagagaaaa ccctgcaacc gcgcagcgaa caggcacaac
    15541 gctttgaaca gctttatcgc cgctatcagc aatgggcgat gagcgccgaa caacactatc
    15601 ttccaacttc cgccccggca caggctgccc aggccgttgc gactctataa ggacacgata
    15661 atggcgattt ttgataatta tgaagtgtgg tttgtcattg gcagccagca tctgtatggc
    15721 ccggaaaccc tgcgtcaggt cacccaacat gccgagcacg ttgttaatgc gctgaatacg
    15781 gaagcgaaac tgccctacaa actggtgttg aaaccgctgg gcaccacgcc ggatgaaatc
    15841 accgctattt gccgcgacgc gaattacgac gatcgttgcg ctggtctggt ggtgtggctg
    15901 cacaccttct ccccggccaa aatgtggatc aacggcctga ccatgctcaa caaaccgttg
    15961 ctgcaattcc acacccagtt caacgcggcg ctgccgtggg acagtatcga tatggacttt
    16021 atgaacctga accagactgc acatggcggt cgcgagttcg gcttcattgg cgcgcgtatg
    16081 cgtcagcaac atgccgtcgt taccggtcac tggcaggata aacaagccca tgagcgtatc
    16141 ggctcctgga tgcgtcaggc ggtctctaaa caggataccc gtcatctgaa agtctgccga
    16201 tttggcgata acatgcgtga agtggcggtc accgatggcg ataaagttgc cgcacagatc
    16261 aagttcggtt tctccgtcaa tacctgggcg gttggctatc tggtgcaggt ggtgaactcc
    16321 atcagcgacg gcgatgttaa cgcgctggtc gatgagtacg aaagctgcta caccatgacg
    16381 cctgccacac aaatccacgg cgaaaaacga cagaacgtgc tggaagcggc gcgtattgag
    16441 ctggggatga agcgtttcct ggaacaaggt ggcttccacg cgttcaccac cacctttgaa
    16501 gatttgcacg gtctgaaaca gcttccaggt ctgcccgtac agcgtctgat gcagcagggt
    16561 tacggctttg cgggcgaagg cgactggaaa accgccgccc tgcttcgcat catgaaggtg
    16621 atgtcaaccg gtctgcaggg cggcacctcc tttatggagg actacaccta tcacttcgag
    16681 aaaggtaatg acctggtgct cggctcccat atgctggaag tctgcccgtc gattgccgta
    16741 gaagagaaac cgatcctcga cgttcagcat ctcggtattg gtggtaagga cgatcctgcc
    16801 cgactgatct tcaataccca aaccggtcca gcgattgtcg ccagcctgat tgatctcggc
    16861 gatcgttacc gtctgctggt taactgtatc gacacggtga aaacaccgca ctccctgccg
    16921 aaactgccgg tggcgaatgc actgtggaaa gcgcaaccgg atctgccaac tgcttccgaa
    16981 gcgtggatcc tcgctggtgg cgcgcaccat accgtcttca gccatgcgct gaacctcaac
    17041 gatatgcgcc agttcgccga gatgcacgac attgaaatca cggtgattga taacgacacc
    17101 cgcctgccag cgtttaaaga cgcactgcgc tggaacgaag tgtattacgg atttcgtcgc
    17161 taagtagcca catcaggtat gtaacgcctg atgcgacgct gacgcgtctt atctggccta
    17221 cacgctgcga ttttgtaggc cggataagca aagcgcatcc ggcattcaac gcctgatgcg
    17281 acgctggcgc gtcttatcag gcctacgcgc tgcgattttg tagaccggat aagcgaagcg
    17341 catccggcac gaaggagtca acatgttaaa agatctcaaa cgccaggtat tagaagccaa
    17401 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    17461 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    17521 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    17581 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    17641 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag gcgggtcagt cgattccagc
    17701 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    17761 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    17821 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    17881 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    17941 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    18001 gctgctgcat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    18061 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    18121 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    18181 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    18241 gcaccgcaag agtccgtggc gtttattcac gccgatcagg ttccccgcgc tcagcatatt
    18301 ttgcagggtg aacaaggctt tcgtctgaca ccgctggcgt taaaggattt tcaccgccag
    18361 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgtctg
    18421 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    18481 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    18541 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    18601 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    18661 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    18721 tacgtcgcca gccgcccgct gctgctggaa aaactcaacg cctggtttgc aaaccacgat
    18781 cccgatgtga tcatcggctg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    18841 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    18901 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    18961 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    19021 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    19081 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    19141 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    19201 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    19261 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    19321 cacgccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    19381 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    19441 gggctggttg aaggcatggc gcaacctgat ccagagcaca gtaccgaagg ttttctcgat
    19501 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    19561 cgcgataaag ccaaacgtca gggtaataag ccgctttcgc aggcgctgaa gatcattatg
    19621 aatgcctttt atggcgtgct cggcaccacc gcctgccgct tcttcgatcc gcgcctggca
    19681 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaga ccaaagcatt gattgaagcg
    19741 cagggctacg acattatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    19801 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    19861 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    19921 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    19981 tacgccgggc tgattcagga gggcgacaag cagcggatgg tgtttaaagg gctggaaacc
    20041 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctgta cctgcgtatc
    20101 ttccgcaacg agccatatca ggagtacgtc cgccaaacca tcgacaaact gatggcgggt
    20161 gaactggatg cgcggctggt ttaccgtaaa cgccttcgcc gtccgctgag cgaatatcag
    20221 cgtaatgttc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa tcaaaagcgc
    20281 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    20341 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    20401 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    20461 accgggcaac ttgggctatt ttgagcaaaa aaaagagttc gccagatacc attttgatgc
    20521 gtgacgaatg ctttgccatc cagtaccata gcgccctttc cattcctgga cctgaataac
    20581 accactacct cataagcacg gtagcgggtg gttattgcct gcaattaaag atatagagcc
    20641 gaacacatat gccttttaca cttggtcaac gctggatcag cgatacagaa agcgaattgg
    20701 gacttggaac cgttgtcgcg gtggatgcgc gaactgtcac tttacttttc ccatctactg
    20761 gtgaaaaccg tctgtacgca cgcagtgatt cccccgtgac ccgcgtgatg ttcaaccctg
    20821 gtgataccat taccagccat gacggctggc agatgcaagt cgaagaagta aaagaagaaa
    20881 atggcttgct gacctatatc ggtactcgcc tggatactga agagtccggc gtagccctgc
    20941 gtgaagtttt ccttgatagc aaactggtgt tcagcaaacc gcaggaccgt ctgtttgccg
    21001 ggcagattga ccgtatggac cgctttgcgc tgcgttatcg cgcgcgtaag tattccagcg
    21061 aacagttccg tatgccgtac agcggcctgc gcggtcagcg taccagcctg atcccgcatc
    21121 agctcaacat cgctcatgat gttggtcgcc gccacgcgcc gcgcgtcctg ctggctgacg
    21181 aagtgggttt agggaaaacc attgaagccg ggatgatcct gcatcagcaa ctgctctctg
    21241 gcgctgctga acgtgtgctg attatcgtcc cggaaacctt acagcatcag tggctggtag
    21301 aaatgctgcg ccgtttcaac ctgcgctttg ctctgtttga tgatgagcgt tatgccgaag
    21361 ctcagcacga tgcttacaac ccgtttgaca ccgaacagct ggtgatttgc tcgctggatt
    21421 ttgcccgtcg tagcaaacag cgcttggaac atctctgtga agccgaatgg gacctgctgg
    21481 tggtcgatga agcgcatcac ctggtgtgga gcgaagatgc gccaagccgt gaatatcagg
    21541 ccattgaaca actggcagag cacgtgccgg gcgttctgct gctgaccgcg accccggaac
    21601 agctggggat ggaaagccac ttcgcccgtc tgcgtctgct ggacccgaac cgtttccacg
    21661 attttgcgca gttcgttgaa gagcagaaaa attatcgtcc ggttgcggac gccgttgcca
    21721 tgctgctggc aggtaacaaa ctgagcaatg acgaactgaa catgctcggc gagatgatcg
    21781 gcgagcagga tatcgagccg ctgttgcagg cagcaaacag cgacagcgaa gatgcccaga
    21841 gcgcccgtca ggagctggtt tcgatgctga tggatcgcca cggcaccagc cgcgtgctgt
    21901 tccgtaacac gcgtaacggt gtgaaaggat tcccgaaacg cgagctgcac accattaagc
    21961 tgccgctacc gacgcagtat cagacggcta ttaaagtctc cggcattatg ggcgcacgta
    22021 aaagtgcgga agatcgtgct cgcgatatgc tctacccgga gcgtatttat caggaatttg
    22081 aaggtgataa cgccacctgg tggaacttcg atccgcgcgt tgagtggctg atgggctacc
    22141 tgaccagcca tcgctctcag aaagtgctgg tgatctgcgc caaagctgcc actgcgctgc
    22201 aactggagca ggtactgcgc gaacgtgaag gtattcgcgc tgcggtgttc cacgaaggta
    22261 tgtcgattat cgaacgtgac cgcgctgccg cctggtttgc cgaagaagac accggcgcac
    22321 aggtactgct gtgctcagaa atcggttctg aaggacgtaa ctttcagttc gccagtcaca
    22381 tggtgatgtt tgacctgcca ttcaacccgg atctactgga gcagcgtatt ggtcgtctgg
    22441 atcgtatcgg ccaggcgcac gatattcaga tccatgtgcc ttatctggag aaaaccgctc
    22501 agtcggtgct ggtgcgctgg tatcacgaag gtctggatgc atttgagcac acctgcccga
    22561 ccggacgcac tatttacgat agcgtataca acgatctgat taactatctg gcttcaccgg
    22621 atcaaaccga aggctttgac gatctgatca aaagctgccg cgagcaacat gaagcgctga
    22681 aagcacagct ggaacagggt cgtgaccgcc tgctggaaat ccactccaac ggtggcgaaa
    22741 aagcccaggc actggcagaa agcattgaag agcaggatga cgataccaac ctgatcgcct
    22801 tcgccatgaa cctgttcgat attatcggta tcaatcagga cgatcgcggc gacaacatga
    22861 tcgtgctgac gccgtccgat catatgctgg tgccggactt ccctggcctg tcggaagatg
    22921 gcatcaccat cacctttgat cgtgaagtgg cgctggcgcg tgaagatgca cagtttatta
    22981 cctgggagca tccgctgatc cgcaacggtc tggatctgat cctttctggc gataccggta
    23041 gcagcacgat ttcactgtta aaaaacaaag cgttgccggt aggtacgctg ttggtggaac
    23101 tgatttatgt ggttgaagcc caggctccga agcagttgca gctcaaccgc ttcctgccac
    23161 cgacgccggt acgtatgctg ctggataaaa acggcaacaa cctggcggcg caggtagagt
    23221 ttgaaacctt taaccgccag cttaacgcgg ttaaccgtca caccggcagc aaactggtta
    23281 acgccgtgca gcaggatgtt cacgctatcc ttcaactggg tgaagcgcag atcgagaaat
    23341 ctgcccgtgc attgattgat gcagcgcgta acgaagccga cgaaaaactg tctgccgagc
    23401 tgtctcgtct ggaagctctg cgtgcagtga acccgaacat tcgtgacgac gaactgaccg
    23461 ccattgagag caaccgtcag caggtaatgg aaagcctgga tcaggcaggt tggcgtctgg
    23521 atgccctgcg tttgatcgtt gtaacgcatc agtaacggag ccgaaaatgg ggatggaaaa
    23581 ctacaatcca ccgcaggaac cctggttggt tatcctgtat caggatgacc atattatggt
    23641 ggtcaacaag ccgagcggtt tgttgtcagt gccgggtcgt ctggaagagc acaaagacag
    23701 cgtgatgacg cgcattcagc gtgattatcc gcaggcagaa tcggtgcatc gtctggatat
    23761 ggctaccagc ggcgtgattg tagtggcgct gaccaaagcc gcggagcggg agttaaaacg
    23821 ccagttccgc gagcgcgagc cgaaaaagca gtatgtggcc cgcgtctggg ggcatccatc
    23881 ccccgcagaa ggtctggtgg atctgccgct gatttgcgac tggccaaacc gcccgaaaca
    23941 gaaagtctgt tacgaaacgg gtaaacctgc gcagacggaa tatgaagtgg tggagtatgc
    24001 ggcggataac acggcaagag tggtgttaaa accgattacc gggcgttcgc atcaattgcg
    24061 tgtgcatatg ctggcgctgg ggcatccgat tctcggcgat cgtttttatg catcaccaga
    24121 agcgagagcg atggcaccgc gtttgttgct acatgcagag atgttgacga ttacccatcc
    24181 ggcgtatggc aatagtatga cgtttaaagc gccagcggat ttttaatcgt cacgctggcg
    24241 cagttcggta ggcggataag gcgttcacgc cgcatccggc aattgtgcgc cgattgcctg
    24301 atgcgacgcg ggccgcgtct tatcaggcct acagtttaaa acttatttaa accctttctg
    24361 ctgctttatc agctcatatg cctgctgaat ttcctgcgct ttctgcttcg ccatctccat
    24421 catctcaggc ggcaaacctt tcgccaccag cttatcggga tggtgttcac tcatcagctt
    24481 acggtaggca cgtttgatgg tggtcgcatc atccgtcggc ttcacgccca gcacattaca
    24541 ggcatcttcc agcgtcgggc cacgctgcgc ttgctgccag ttaccaccgc cagattgctg
    24601 ctgataaccg ccgccaaact gtgcaccgcc ctgcatcatg cgcaaaaact ggtcaaactg
    24661 ggcgcgggag atccctaatt cttcagcaat gacatacagc accgcccgtt cattcgggtg
    24721 cagtgaacca tcagcaaacg ccgcctgaat ctggatctcc agaaacatac gaattaagtc
    24781 aaaacgacca aagcagacac tgcgaaactg gcgcatcttt tcgcgcagcg ggtaattatc
    24841 tgattttccc acccggaacg cgttttgcgc cgcagtacgg gaagcgccat gaaggttcat
    24901 tcggtccatc aactggctgg cgatatgaat atcagcctcc gtgacgcgac ctttggattt
    24961 ggttaaatgc cccatcactt caaaagtggt ggcaaaaaac agcgcctgac gctcacgctg
    25021 gttggcgaac cacgccattt tacggctacg ggctttatca aacatatggc caattaacag
    25081 gcctaacact acgccccaaa agccgccgcc catcagtaag gccacggcca cgccaatgat
    25141 ttttccccaa tactgcatat attccccaaa tcgacacacg gatatcaggg ctatctccca
    25201 caatataaag gtgcttttac cgttttccgg ctgcggtcaa ttgtgggaca tcgcctataa
    25261 tttgcattat catacctgtc attcaatgcc gtgcctaaca ccactgacgc tattcggaca
    25321 ggattaacac tagcgtagag atgacgagta cgttagtctc tgaccgtttg tcacgcgcaa
    25381 cgttaccgat gatggaacaa taaaatcaac gtatgaaaaa acgtatcccc actctcctgg
    25441 ccaccatgat tgccaccgcc ctttatagtc aacagggact ggcagccgac ctcgcctcac
    25501 agtgcatgtt gggcgtgcca agctatgacc gtcctctggt acagggcgat accaatgact
    25561 tacccgtgac tatcaatgct gaccacgcga aaggggacta cccggatgac gccgtgttta
    25621 ctggcagcgt ggatatcatg cagggtaaca gccgtctgca ggccgacgaa gtgcagctcc
    25681 atcaaaaaga ggcaccagga caaccggagc cggtacgtac cgttgatgcg ctcggtaatg
    25741 tccattacga cgataaccag gtgatcctca aagggccgaa aggctgggcg aatctgaaca
    25801 ccaaagatac caacgtctgg gaaggtgatt accagatggt gggtcgccag ggtcgcggta
    25861 aagcggacct gatgaaacaa cgtggcgaaa accgctatac cattctggat aacggtagct
    25921 ttacctcctg tctgccgggt tctgacacct ggagcgtggt aggtagcgaa attattcatg
    25981 accgcgaaga acaagttgct gagatctgga acgcccgctt taaggtgggt ccggtaccga
    26041 tcttttatag cccctatttg cagttgccgg tgggtgacaa acgtcgctct ggtttcttga
    26101 tcccgaacgc caagtacacc accaccaact actttgagtt ctacctgcca tattactgga
    26161 acatcgcgcc aaatatggat gccaccatca cgccgcatta tatgcatcgt cgtggcaaca
    26221 tcatgtggga gaacgaattc cgctacctct cccaggcggg cgctggcttg atggaactgg
    26281 actatctgcc ttcagataaa gtctatgaag atgaacaccc gaacgatgac agttcacgtc
    26341 gttggttatt ctactggaac cactccgggg tcatggatca ggtgtggcgt ttcaacgtcg
    26401 actacaccaa ggtcagcgat cctagctact tcaatgattt cgataacaag tacggttcca
    26461 gtactgacgg ctacgcaacg caaaaattca gcgttggcta tgcggtgcaa aacttcaatg
    26521 ccaccgtttc aaccaagcag ttccaggttt tcagcgaaca gaacaccagt agctactcgg
    26581 cagagccgca gttagacgtt aattactacc agaatgatgt tggtccgttt gatacgcgta
    26641 tttacggcca ggcagtgcac tttgttaaca ccagagacga catgcctgaa gcaacccgtg
    26701 ttcacctgga accgaccatc aatttgccgc tctctaataa ctggggcagc atcaataccg
    26761 aagcgaagtt tctggcaacc cattatcagc aaaccaatct tgactggtat aactccagaa
    26821 acacgaccaa gctggacgaa tccgttaacc gcgtaatgcc gcaattcaaa gttgacggca
    26881 aaatggtctt tgaacgcgat atggaaatgc tggctccggg ttatacccaa acgctggaac
    26941 cgcgcgcgca gtatttgtac gtgccgtatc gcgatcagag cgacatctat aactacgact
    27001 cgtctctgct gcaatctgac tactctggcc tgttccggga ccggacttac ggcggtcttg
    27061 accgtattgc ctccgctaac caagtgacga ccggtgtcac atctcgcata tatgatgatg
    27121 ctgccgttga acgttttaat atttccgttg gtcaaatcta ctatttcacg gagtctcgca
    27181 ctggcgatga caacataaca tgggagaatg acgacaaaac gggctcactg gtgtgggcag
    27241 gcgacaccta ctggcgtatc tccgagcgtt ggggattgcg tggcgggatt cagtacgata
    27301 cacgtctgga taacgtagcg accagtaact ccagcattga ataccgtcgg gatgaagacc
    27361 gtctggtaca gctgaattac cattacgcca gcccggaata tattcaggct acgctgccta
    27421 agtactattc cactgctgag caatataaga atggtatttc gcaggtaggt gctgtcgcca
    27481 gcaggccaat tgccgatcgt tggtccattg ttggggccta ctactacgac accaatgcga
    27541 acaagcaagc cgactctatg ttaggtgtgc aatacagctc ctgctgctat gcaattcgcg
    27601 tcggttacga gcggaagctg aacggttggg ataacgataa acaacatgcg gtatatgaca
    27661 acgcaatcgg ctttaacatc gaacttcgcg gcctgagctc caactacggt ctgggtacgc
    27721 aagagatgct gcgttcgaac attctgccgt atcaaaacac tttgtgattt gttgatttac
    27781 cacgtaatcc gcagtgcggt taattgaaat ggaaaaagta tgaagaactg gaaaacgctg
    27841 cttctcggta tcgccatgat cgcgaatacc agtttcgctg ccccccaggt agtcgataaa
    27901 gtcgcagccg tcgtcaataa cggcgtcgtg ctggaaagcg acgttgatgg attaatgcag
    27961 tcggtaaaac tgaacgctgc gcaggcaagg cagcagcttc ctgatgacgc gacgctgcgc
    28021 caccaaatca tggaacgttt gatcatggat caaatcatcc tgcagatggg gcagaaaatg
    28081 ggagtgaaaa tctccgatga gcagctggat caggcgattg ctaacatcgc gaaacagaac
    28141 aacatgacgc tggatcagat gcgcagccgt ctggcttacg atggtctgaa ctacaacact
    28201 tatcgtaacc agatccgcaa agagatgatt atctctgaag tgcgtaacaa cgaggtgcgt
    28261 cgtcgcatca ccatcctgcc acaggaagtc gaatccctgg cgcagcaggt gggtaaccaa
    28321 aacgacgcca gcactgagct gaacctgagc cacatcctga tcccgctgcc ggaaaacccg
    28381 acctctgatc aggtgaacga agcggaaagc caggcgcgcg ccattgtcga tcaggcgcgt
    28441 aacggcgctg atttcggtaa gctggcgatt gctcattctg ccgaccagca ggcgctgaac
    28501 ggcggccaga tggggtgggg ccgtattcag gagttgccgg ggatcttcgc ccaggcatta
    28561 agcaccgcga agaaaggcga cattgttggc ccgattcgtt ccggcgttgg cttccatatt
    28621 ctgaaagtta acgacctgcg cggcgaaagc aaaaatatct cggtgaccga agttcatgct
    28681 cgccatattc tgctgaaacc gtcgccgatc atgactgacg aacaggcccg cgtgaaactg
    28741 gagcaaattg ctgctgatat caagagtggt aaaacgactt ttgctgccgc agcgaaagag
    28801 ttctcgcagg atccaggttc tgctaaccag ggcggtgatc tcggctgggc tacaccagat
    28861 attttcgatc cagccttccg tgacgccctg acccgcctga acaaaggtca aatgagtgca
    28921 ccggttcact cttcattcgg ctggcattta atcgaactgc tggatacccg taatgtcgat
    28981 aaaaccgacg ctgcgcagaa agatcgtgca taccgcatgc tgatgaaccg taagttctcg
    29041 gaagaagcag caagctggat gcaggaacaa cgtgccagcg cctacgttaa aatcctgagc
    29101 aactaatggt taaaacccaa cgtgttgtga tcactcccgg cgagcccgcc gggattggcc
    29161 cggacttagt tgtccagctt gcacagcgtg agtggccggt cgaactggtt gtttgtgccg
    29221 atgccactct ccttaccgac cgggcagcga tgctcggttt gccgctcacc ctccgccctt
    29281 attcccccaa ctcccctgca caaccgcaaa ctacgggcac attaacgcta cttcctgtcg
    29341 cgctacgtga atctgtcact gcggggcagt tagcgattga aaatgggcat tacgtggtgg
    29401 agacgctggc gcgagcgtgt gatggctgtc tgaacggtga atttgctgcg ctgatcacag
    29461 gccccgtgca taaaggcgtc attaacgacg caagcattcc gtttaccggt cataccgagt
    29521 ttttcgaaga gcgttcgcag gcgaaaaaag tggtgatgat gctggcgacc gaagaacttc
    29581 gcgtggcgct ggcaacgacg catttaccgc tgcgcgatat cgcagatgct atcacccctg
    29641 cacttttgca cgaagtgatt gctattttgc atcacgattt gcgtacaaaa tttggtattg
    29701 ccgaaccgcg cattctggtc tgcgggctga atccgcacgc gggcgaaggc ggtcatatgg
    29761 gtacggaaga gatagacacc attattccgg tgctcgacga gctgcgggcg caggggatga
    29821 aactcaacgg gccgctgcct gccgataccc tgtttcagcc gaaatatctc gataacgccg
    29881 acgccgtgct ggcgatgtac cacgatcagg gtcttcccgt gctaaaatac cagggcttcg
    29941 ggcgcggtgt gaacattacg ctgggcctgc cctttattcg cacatcagtg gaccacggca
    30001 ccgcgcttga actggcggga cgtggcgaag ccgatgtcgg cagttttatt acggcgctta
    30061 atctcgccat caaaatgatt gttaacaccc aatgaataat cgagtccacc agggccactt
    30121 agcccgtaaa cgcttcgggc aaaactttct caacgatcag ttcgtgatcg acagcattgt
    30181 ttctgccatt aacccgcaaa agggccaggc aatggtcgaa atcggccccg gtctggcggc
    30241 attgaccgag ccggtcggcg aacgtctgga ccagctgacg gtcatcgaac ttgaccgcga
    30301 tctggcggca cgtctgcaaa cgcatccatt cttaggcccg aaactgacga tttatcagca
    30361 ggatgcgatg acctttaact ttggcgaact ggccgagaaa atgggtcagc cgctgcgtgt
    30421 tttcggcaac ctgccttata acatctccac gccgttgatg ttccatctgt ttagctatac
    30481 tgatgccatt gccgacatgc actttatgtt gcaaaaagag gtggtgaatc gtctggttgc
    30541 aggaccgaac agcaaagcgt atggtcgatt aagcgtcatg gcgcaatact attgcaacgt
    30601 gatcccggta ctggaagttc cgccgtcagc ctttacacca ccacccaaag tggattccgc
    30661 cgtcgtgcgc ctggtccctc atgcaacgat gcctcacccg gttaaagata ttcgcgtgtt
    30721 gagccgcatc accaccgaag cctttaacca gcgtcgtaaa accattcgta acagcctcgg
    30781 caacctgttt agcgtcgagg tgttaaccgg aatggggatc gacccggcga tgcgagcgga
    30841 aaatatctct gtcgcgcaat attgccagat ggcgaactat ctggcggaga acgcgccttt
    30901 gcaggagagt taacgatgat caattcgccc cgagtgtgta ttcaggttca aagcgtctac
    30961 attgaggcac aatcttcacc tgataatgaa cgttacgttt ttgcttatac cgtaaccata
    31021 cgcaatctgg ggcgagcgcc agtgcagttg ttggggcgtt actggctgat caccaatggc
    31081 aatggccgtg aaaccgaagt ccagggcgaa ggagtggttg gcgtccagcc acttatcgcg
    31141 cctggcgaag agtaccagta caccagcggt gcaatcattg aaaccccgct gggcaccatg
    31201 cagggtcact acgaaatgat cgatgaaaat ggcgtccctt tcagcatcga cattcccgta
    31261 ttccgactcg ccgttcccac actcattcat taaaagaata tggcgacata ccttattggc
    31321 gacgttcatg gttgttacga tgaactgatc gcattgctgc ataaagtaga atttaccccc
    31381 gggaaagata ccctctggct gacgggcgat ctggtcgcgc gcggcccggg ttcgctggat
    31441 gttctgcgct atgtgaaatc cttaggcgac agcgtacgtc tggtgctggg caatcacgat
    31501 ctgcatctgc tggcggtatt tgccgggatc agccgcaata aaccgaaaga tcgcctgaca
    31561 ccgctgctgg aagcgccgga tgccgacgag ctgcttaact ggctgcgtcg ccagcctctg
    31621 ctgcaaatcg acgaagagaa aaagctggtg atggcccacg cagggatcac gccgcagtgg
    31681 gatctgcaga ccgccaaaga gtgcgcgcgc gatgtagaag cggtgctgtc gagtgactcc
    31741 tatcccttct ttcttgacgc tatgtacggc gatatgccaa ataactggtc accggaattg
    31801 cgggggctgg gaagactgcg ttttatcacc aacgccttta cccgtatgcg tttttgcttc
    31861 ccgaacggtc aactggatat gtacagcaaa gaatcgccgg aagaagcccc tgccccactg
    31921 aaaccgtggt ttgcgattcc tggccctgtc gctgaagaat acagcatcgc ctttggtcac
    31981 tgggcatcgc tggaaggcaa aggtacgccg gaaggtattt acgcgctgga taccggctgc
    32041 tgctggggtg ggtcattaac ctgcctgcgc tgggaagata aacagtattt tgtccagccg
    32101 tcgaaccggc ataaggattt gggcgaagcg gcggcgtctt aaacacagcc tgatacagga
    32161 aggccggata agacgcgacc ggcgtcgcat ccggcactag ccgtaaattc tatacaaact
    32221 taccgccgct ccagaatctc aaagcaatag ctgtgagagt tctgcgcatc ggcgtcgtgg
    32281 aattcgctga ataccgattc ccagtcatcc ggctcgtaat ccgggaaatg ggtgtcgcct
    32341 tccacttctg cgtcgatatg cgtcagatac agtttttgcg cttttggcag gaactgctca
    32401 taaacgcgac cgccgccaat caccatgatt tctggtacgt caccacacgc cgcaatggct
    32461 tcatccaccg atttcaccca cgttacgcga tcgtccgtac ccggttgact gctgaggata
    32521 atatttttgc gtcctggcaa cggacgaccg attgattccc aagtatggcg gcccataatc
    32581 acgggtttat ttaaggtgtt gcgtttaaac caggcgagat cggcaggcag gttccacggc
    32641 atggcgtttt ccatgccgat aacgcgatct accgctaacg ccgcaatcag actgatcatt
    32701 gagatttccc gataaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    32761 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctccccct
    32821 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    32881 cactctttat taagatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    32941 accttcttct gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    33001 ggtgatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    33061 gtcattctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    33121 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagcgcca gacgcccggt
    33181 tttcagcgca ccttcaaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    33241 aatgtagtga tcgacatcgc gggcgcgggc aataatctgc aaatgcggga aatgttcttt
    33301 caccatctcc gtcagttgca ggttggtttg cggatcgtca atagcgttaa tcagcacttc
    33361 cgctttcgcc gctccggcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    33421 tttcatacca aatttacgca gcgtttcgat atggtccgga tcgtgatcga gcaccaccat
    33481 tttcaccccg ctggagagca gtaaacgtcc ggtaatctgt ccaaaacgac cgaatccggc
    33541 gataatcacg cgcggctgtt cttcatcgat ctcatcggct tcacgcgcct cctccgtaga
    33601 agattgctca aggcgattga ggatcaccag cagaatcggc gttgctgcca tcgacagcgc
    33661 caccgccagg gtcagcgatt tcacccactc cggctccagc acattcgcca tctgcgccgc
    33721 accaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accagcgacg
    33781 ctgtttattt ggcacctgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    33841 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaatcagcg tgccaaagtc
    33901 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa acggttcgat
    33961 atcgctttcc aacgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    34021 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacaaggaa
    34081 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gcaacaaaac gcagcgccgg
    34141 acgcgtaaca tagcgcccca gcaataccac cagtaccagc gcacccgcca cttttaacgc
    34201 cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gccgccagta gcgggatcat
    34261 cgccaccagc gggattgccg cgatatcctg gaacagcagc actgcaaagg cactgcggcc
    34321 catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgttgatga
    34381 gagcgccagc gtcataccga tcaattccgc gacctgccag cgcagcccaa gtaacataca
    34441 gaacagcccc agcaggccac cgcaaatcac catctgcaat gcgccaccgc cgaacactgc
    34501 cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag
    34561 caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc
    34621 ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg
    34681 taccgcaatg ggtacaatca gcgccgccga tccgaggtag atcagcgcct gaaccagcgt
    34741 atgactatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag tggcgcgcct
    34801 gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc
    34861 agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg
    34921 gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat
    34981 gtttgccatg cagcgccgtg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat
    35041 cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcgggtgc tgccagacga
    35101 tcagatcggc gcgagagagc gcctcctgct cggcggcaat atcgatattg aagtcaggat
    35161 agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc
    35221 gtttattcgc atgggaatga tgcggatacg gatgcgcata aatgatgagg atcatagtta
    35281 ttagcccgcg tgtttacgcc tttgttttcc cacaaagtgt agtcatatat tcacgcggct
    35341 tatagtcaat aatattgatt cagaaaagag agaaatctga acaaataatc ccattctctg
    35401 cccgcggatc gtcatattgc tggtctgcgc cagtgacatt cctcgagtct ccggggcaaa
    35461 cgctacggaa atcaacaagc caaacagcga gatacccgcc cccattagca tcgtgttact
    35521 gataccgtaa ttattgataa atatcggcag tgcccaggtc gaaacaatgg tgccaatacg
    35581 acttaaggac ataatcacgc ccacggcaga ggcgcggata tccgtcggga agagttcatt
    35641 aggatagagc cactgcaaat tacccggccc gccagagaaa aaggcataca ccgcaaaggc
    35701 catcactacc agccagatcc ccatatccgg gattagcccc aaaaccgcca acgccagcgt
    35761 catcatggca aagctgccaa tcaacaatgg acgccgtccg gcagtgttta gccacagcat
    35821 cggcggaata cagccgagca taaagaacag gctaatcacc acattcccta gtgccgcgtt
    35881 tttgccaacc cccaatccca acgaaccaac gatttgcggg ccaaaggtgt aaatggcgaa
    35941 cattgggatc acctggcagg tccagatggc ggcaacaaac agaacaaaag gaaaatggcg
    36001 gcgattaaac agatcgcgaa aacgggtttg ctgcggctgc tcttcatcga aagccaccgg
    36061 ttcgccaaac agttttatca tcatctcttc gcactctttt actcgccctt tgcgtaataa
    36121 ccagcgggga gattctggca gttcgaatcg accaatcaaa atcaacaaac aagggatcgc
    36181 cgcgctaccc agcatccagc gccaaccacc ttccacatca taaagccagt agccgaccag
    36241 atcggcgcag gtcgcgccga cataccacat cgcggcaata aagctgatgg aaaacgcccg
    36301 ctgacgggta ctggagaact cggtgatcat cgaagtggcg atgggataat ctgcaccgat
    36361 gacaatgccg atgagtaccc gcatcaccaa cagttcgacg ggggatgaaa cacacatcgt
    36421 cgccaccgat atcacgccga tggcgatgat atcaatgagg aacattttgc gccgtccgac
    36481 tttatcggag atatagccaa acagcgatgt gccaacgaac agcccggcga gcgttcccgc
    36541 gcccagcaag ccaatccagt cagcgtccag tttcagcgcc ggagtcagtt gctccagcgc
    36601 cacgccaatc attaccagta cataaccatc cagaaacgga ccaccgcttc cccacagcaa
    36661 aatgcggcgg tgaatagagg agaatttgag atcgtcaaag tttctggacg gttgcatggg
    36721 ttattcctgt ttttttgcaa tgagagtcag tacggcgtta cgtatcaaac caacatcagc
    36781 cgtaacggaa ctccacacca aaggtgccgc gcgggtattc ccactgttcc agcgccgacc
    36841 ccagccccag aatgcgacag gtgccgcact ccagacatcc ggcgtaatcg aagcgcacac
    36901 tgccgtcatc ctgcttcttg tacagacctg cggggcacgc tttcaccagc agctccagca
    36961 cctgtttatc agcatcggcc ttcacaacaa tgtgcggatg ctcttcatcg acattgaatt
    37021 tattgacgcc cagtttgacg tccacattga cgggagaagt cataaaacgg tcactccttt
    37081 catgccatcc ttgatcagat tgatgaagcc cactttcttg ccgtggcgga ggattttctt
    37141 gcgcatcagt tccggcgcac tgccgtcaat ggtgaacagg tcacgcgcca cgcccaccgc
    37201 cagttccggg ttgccgctaa acatgcgtgg gttatcaagg aacgccggta gtttctggta
    37261 catgcgcata tcgcgcagcg ggccactctc cagatgctga cgatattccg cgagtttttg
    37321 cttactgaaa tcgtcgcttt tcatcgctga aagcacggtt tttgctgcag cttccccggc
    37381 ggcaatcgcc agatccatac cgcgaatggt aaaaccgagg ttcatacaca ttccggcggc
    37441 atcaccggca atcaatacgc cgtcaccgac cagctccggc agcatgttga tgcctgcttc
    37501 cggcactacg tgagcggaat attccaccag cttgccgccc gcgatcagcg gtgcaacggc
    37561 cggatgctgt ttgaaatctt ccagcatttg cggcaccgat tttttcgcgt catgcagatg
    37621 atgcagacca caaaccagcc ccagcgacag ggtattttca ttggtataaa ggaagccgcc
    37681 gcccatcagg ccatcggtgg gtgatcccgc aaacagacaa gccgcgccct gattaccctg
    37741 caactgaaaa cggtcttcga ttaccgactt cggtaactcg atcagttcct tcacgccaac
    37801 cgccacatcc gtcggtttaa cgcgttttgc catccccaac ttttcggcaa ggatggagtt
    37861 caccccatca gcaaggagca ccgttttcgc ttcaatcaca tcaccatcgg cttctacacc
    37921 gacgactttg ccatcgcgct gtacgaggtt atcgacgcgg atcccggtaa ttaactgcgc
    37981 gcccgcttct tcggcctgct ccatcaacca ggcatcaaat ttactgcgca aaacggagta
    38041 agaacgctgg gatggcgagg tttcgtcacc gttgcagtag tccatagtca tcgctgactt
    38101 ttccgtcata aacgcgagtt tttcatgggt gatcaggcgt tctacggggg cggaatcggc
    38161 gaaacctgga ataatgtgtt ccagactgtg ggcatagaga cgcccgccag taacgttctt
    38221 ggcacctgcg gaattgccac gctcgataac taacacttgc gccccttcgc gggcgagcac
    38281 cagtgcggca accgaaccgg caagccctgc accgacgatg atggcgtcaa agatatcttc
    38341 ggacataaga gctccctgtc agcggcagag ccgtaatgca gccctgccag agtggatcaa
    38401 cgcgctaaag ctgcggtcag tgccggaagg atcttcacgg catcgccaac aatgccgtaa
    38461 tccgcgtact ggaagatcgg cgcattttta tctttattga tggcgaaaat ggtttgcgac
    38521 gcgttagcgc caaccatgtg ctggatctac ccggagatcc ccaccgccag gtacagttca
    38581 ggtttcagca tcaggttgga gataccgaca tagcgttcgt gctccatcca tttttcgttt
    38641 tctgccaccg gacgagaaca ggccaactcc gcacctatcg ccttgcaaag ctgttctgcc
    38701 agcgcaatgt tctctttgct gccaatgccg cgaccgacgc tgaccaccag acgggctttg
    38761 tcgagatcga cgctgttgct ctggcgcgcc tgggttgccg tgcgggtaat cgccacagcc
    38821 ggagcctgcc actccacggt gtgcgtttcg ccagtgcgtg acgcgtctgg ctgagccgca
    38881 tcgaacgtgc cgctgctgat ggtcagtacc gcatacggcg tggcaatgcg ttcttcgcca
    38941 atcgccagac cgccgtaaac catgtgtttc actgttgctt taccgtcctg tacgctgacg
    39001 gtgctggcat cgttagacac cgccgcttta aggcgataac ccagttttgc cgccagtaat
    39061 ttgccgcgac gggtgtttgg caacagcacc agaccgtcag cgccgtgctg gcgaatagtg
    39121 tcagccatga caccggcgta atcttcgatc atccgatcgt ccggtttgcc gtttaatttc
    39181 cagacatgat tagcgccgag ctggattgcc tgtgcgccgt cggcatcatt gaggacaaag
    39241 gcattgattt gattagctaa agcctgcgca ccgttcatca gttccggcag acgagagggg
    39301 gtatcgctga atacccagac ttgagaaaac gtgttcatag catcccctgt aattaaatga
    39361 ctttgcgaag attttcagca aatgcggcga tctgttcttc gccgtcgcct tcaatcacga
    39421 tgcgctgacg ctcgcgctgt ttcggcgcgg caacctgttg ttcagaccag gcgtccacgg
    39481 cgttaaaacc aatatccgcc gccgaccata cctggactgg ctttttcgcc gcgccgagaa
    39541 tggctttcat cgaaggaatt tgtggggagt tgatatcagt ggaaacagca acaaccgcag
    39601 gcagcggaat gcttaaggtt tcggtttcat cttccagttc gcgctcaacg gtgagggtat
    39661 ctgccgtcag ggagataatt ttgctgacgc cgttaactgc cggaatattg aggatttcgc
    39721 ccaccagcag accaacctgc tgggcataaa ggtcggaaga accatcgcca cagaggatca
    39781 gatcaaagcc tgctttctgg gcggctgcag ccagtgcgct cgccgtttgt tgcggcagtg
    39841 cctgctcgaa ctggtcatca atcaccacaa tcagttcatc cgggccgcgc gatagcacat
    39901 ctttacgccc tttggcgttg gtcagggctt taccgcccac acttaaggct gtcacctgcg
    39961 cctctgctgc ctgttgcttt agctggcaag ccgcttcaat agcgttgaga tcgtattggc
    40021 ttattttggc atcggctttg ctgaagtcta atgaaccatc agcattattg accgcaatat
    40081 cctgttcatc aggcacgcac ttatagcaag taataatctt cattgcatct ccagaaatca
    40141 tgaaggaaac gtcgttcata tcggtggtgt taattacaga tatcacgccc tttaatttgg
    40201 aaataaaact ttaatcacca atattgaaag tcagatgcta tttttttacc cacaaaagaa
    40261 tataacaaac tgattaataa ccaaaattaa cgaaaaacgc cctctgttca ctcgaaacaa
    40321 aaatgtgata ccaatcacag aatacagctt attgaatacc cattatgagt tagccattaa
    40381 cgcgtccacg atgttaataa taattatatt aaatgttaac aaaaataaaa caaacgggaa
    40441 cgcaaaataa atattcgttt tcacagtgga attaactcat gaagaatgaa aagagaaaaa
    40501 cgggaataga accgaaggtt ttctttccgc cattaataat cgtcggcata ctttgttggc
    40561 ttacagtcag agatctggat gcggcgaatg tcgttattaa tgctgtattc agttacgtca
    40621 ccaatgtatg gggatgggca tttgaatggt atatggtggt gatgcttttc ggttggttct
    40681 ggctggtgtt tggcccgtat gccaaaaagc gtttaggtaa cgaaccgcca gaatttagca
    40741 ccgccagttg gatctttatg atgttcgcct cctgtacgtc tgctgccgta ctgttctggg
    40801 gatcgattga gatctactac tacatctcca ccccgccgtt tggcttagaa ccgaactcga
    40861 caggagcgaa agagttgggg ctggcttaca gcttgttcca ctggggacct ctgccgtggg
    40921 ccacttacag cttcctttca gtcgccttcg cttacttctt ctttgtccgc aaaatggaag
    40981 tgattcgccc cagctcgaca ctggtgccgc tggcaggtga aaaacacgcc aaagggttgt
    41041 tcggtaccat cgtcgacaac ttctatctcg tcgccttgat cttcgcgatg ggtaccagtc
    41101 tgggccttgc cacgccgctg gtgaccgagt gtatgcaatg gttgtttggc attccgcata
    41161 ccctgcaact ggacgctatc atcattacct gctggattat cctcaacgcc atttgcgtcg
    41221 cttgcggtct gcaaaaaggg gtacgtatcg ccagtgacgt gcgtagttac ctgagcttcc
    41281 tgatgctggg ttgggtgttc atcgtcagcg gtgccagctt catcatgaac tacttcaccg
    41341 attcggtggg gatgttgctg atgtatctgc cgcgcatgtt gttctatacc gatcccatcg
    41401 ctaaaggcgg cttcccgcag ggctggaccg tgttctactg ggcatggtgg gtgatttacg
    41461 ccatccagat gagtatcttc ctcgcccgca tctcccgtgg tcgtactgtg cgtgaactgt
    41521 gcttcggcat ggtgatgggg ctgacagcat ccacctggat cctgtggacc gtgctcggta
    41581 gtaacactct gctgttgatg gataaaaaca tcatcaacat tccaaatctg atcgaacagt
    41641 acggtgtggc gcgcgccatc atcgaaacct gggccgctct gccactcagc accgccacca
    41701 tgtggggctt cttcatcctc tgctttattg ccaccgttac gctggttaac gcctgctctt
    41761 ataccctggc gatgtctact tgccgcgaag tacgcgatgg tgaagaacca ccgctgctgg
    41821 tgcgtatcgg ttggtcaatt ctggttggca ttatcggtat tgttctgctg gcgctcggcg
    41881 gcctgaaacc gattcaaacc gccattatcg ccggaggatg cccgctgttc ttcgtcaaca
    41941 ttatggtgac gctctccttt attaaagacg cgaaacagaa ctggaaagat taattaaccc
    42001 ccaaaatatc aagaggttga aagatggatt ttaatttaaa tgatgagcag gaactgtttg
    42061 tcgccggtat ccgcgaactg atggccagcg aaaactggga ggcctatttt gccgagtgcg
    42121 accgtgacag cgtctacccg gaacgttttg tcaaagcact ggcggatatg ggtatcgaca
    42181 gtctgctgat ccctgaagag cacggtggtc tggacgcggg gtttgttact ctcgccgccg
    42241 tgtggatgga gctgggacgt ctgggggcac caacctatgt actgtaccag ctgccgggcg
    42301 ggttcaatac cttcctgcgc gaaggcacac aagagcagat cgacaagatt atggctttcc
    42361 gcggcaccgg taagcagatg tggaactcag cgattaccga accgggcgcg ggctccgacg
    42421 tgggtagcct gaaaacgact tatacccgta gaaatggtaa gatttatctt aatggtagta
    42481 agtgttttat taccagtagc gcctacaccc cgtacatcgt ggtgatggcg cgcgacgggg
    42541 cttctccgga caaacctgtc tacaccgaat ggtttgttga tatgagcaaa ccgggcatca
    42601 aagtgaccaa acttgagaag ctcggtctgc gtatggatag ctgctgtgaa atcacctttg
    42661 acgatgtgga actggacgag aaagacatgt tcggtcggga aggtaacggc tttaaccgcg
    42721 tcaaagaaga gttcgaccat gaacgtttcc tggtagccct gaccaactac ggtacggcga
    42781 tgtgcgcctt tgaagatgct gcgcgctacg ccaaccagcg cgtgcagttt ggcgaggcta
    42841 ttggtcgttt ccagttgatt caggaaaaat tcgcccacat ggcgatcaaa ttaaactcca
    42901 tgaaaaacat gctgtatgaa gcagcgtgga aagcagacaa cggcaccatc acctctggcg
    42961 atgcagcgat gtgcaaatac ttctgcgcca atgcggcatt tgaagtggtg gatagcgcaa
    43021 tgcaggtgct gggcggtgtc gggattgcgg gcaaccaccg catcagccgc ttctggcgtg
    43081 acctgcgtgt agaccgcgtt tccggaggat ctgacgaaat gcagatcctg acgctgggtc
    43141 gtgcggtgct gaagcaatac cgctaagtcc gtgcgctgcc tgatgcgacg ctgacgcgtc
    43201 ttatcaggcc tacgggagca cgaatgtaag ccggataagg cgtttacgcc gcatccggca
    43261 gtcatgcgct acataaattt ttcaggagct aataatggat catctaccca tgccgaaatt
    43321 cggaccgctg gccggattgc gcgttgtctt ctccggtatc gaaatcgccg gaccgtttgc
    43381 cggacaaatg ttcgcagaat ggggcgcgga agttatttgg atcgagaacg tcgcctgggc
    43441 cgacaccatt cgcgttcaac cgaactaccc gcagctctcc cgccgcaatt tgcacgcgct
    43501 gtcgttaaat attttcaaag atgaaggccg cgaagcgttt ctgaaattaa tggaaaccac
    43561 cgatatcttc atcgaagcca gtaaaggtcc ggcctttgcc cgtcgtggca ttaccgatga
    43621 agtactgtgg cagcacaacc cgaaactggt tatcgctcac ctgtccggtt ttggtcagta
    43681 cggcaccgag gagtacacca atcttccggc ctataacacc atcgcccagg ccttcagtgg
    43741 ttacctgatt cagaacggtg atgttgacca gccaatgcct gccttcccgt ataccgccga
    43801 ttacttttct ggcctgaccg ccaccacggc ggcgctggca gcactgcata aagtgcgtga
    43861 aaccggtaaa ggcgaaagta tcgacatcgc catgtatgaa gtgatgctgc gtatgggcca
    43921 gtacttcatg atggattact tcaacggcgg cgaaatgtgc ccgcgcatga gcaaaggtaa
    43981 agatccctac tacgccggtt gcggtctgta taaatgtgcc gacggctaca tcgtgatgga
    44041 actggtgggc attacccaaa ttgaagagtg ctttaaagat attggcctcg cacatctact
    44101 cggtacgccg gaaatcccgg aaggcactca gcttatccac cgtatcgaat gcccttacgg
    44161 cccactggta gaagaaaaac tcgatgcctg gctggcggca cataccatcg cagaagttaa
    44221 agaacgcttt gccgaactga atatcgcctg tgccaaagta ctgaccgtac cggaactgga
    44281 aagcaatcca cagtatgttg cccgtgaatc aatcactcag tggcaaacga tggatggtcg
    44341 cacctgcaaa gggccgaaca tcatgccgaa attcaaaaat aaccccggac aaatctggcg
    44401 cggaatgccc tcacatggca tggacacggc tgccattttg aaaaatatcg gctacagcga
    44461 aaacgacatt caggagttgg tcagcaaagg tctggccaaa gttgaggact aaacgcgtgg
    44521 cgtcagtcgg cttgcctgac tagcgcccgc ccaataacaa acgaacaaaa atggatagag
    44581 gtgcaatgga tatcattggc ggacaacatc tacgtcaaat gtgggacgat ctggcggacg
    44641 tttacggtca taaaacggcg ctgatttgtg aatccagcgg cggagtcgtt aaccggtata
    44701 gttatcttga gttaaatcag gagattaacc gcacggcaaa cctgttttat acgctgggga
    44761 ttcgcaaagg caacaaggtt gcactacatc tcgacaactg cccggaattt atcttttgct
    44821 ggttcgggct ggcaaaaatt ggcgcgatta tggtgccgat taacgcccgc ctgttacgcg
    44881 aggaaagcga gtggatcttg caaaatagcc aggcgtgcct gctggtgacc agtgcgcaat
    44941 tttatcctat gtatcaacag attcagcagg aagatgccac tcaattgcgg cacatttgcc
    45001 tgacagatgt ggcacttccc gctgatgatg gagtgagttc atttactcaa ctgaaaaatc
    45061 aacaacctgc caccttgtgc tatgcaccgc cgctatcgac tgacgatacg gcggaaattc
    45121 tcttcacctc cggcaccacc tcccgaccga aaggtgtggt gattacccat tacaacctgc
    45181 gcttcgctgg atattactcc gcctggcagt gtgcactgcg tgacgatgac gtctacatga
    45241 cggtaatgcc tgcgtttcat atcgattgcc agtgtactgc ggcgatggcg gcgttttctg
    45301 ccggggccac ctttgtgctg gtcgagaaat acagcgcccg cgccttctgg ggacaggcgc
    45361 agaagtaccg cgccaccatt accgaatgta ttccgatgat gatccgtacg ttgatggtgc
    45421 agccgccttc agcgaacgat cggcaacacc gcctgcggga agtgatgttt tatctcaact
    45481 tgtcggagca ggaaaaagac gcattttgtg aacgcttcgg cgttcgcttg ctgacgtctt
    45541 atgggatgac ggaaaccatt gtgggcatta tcggcgatcg ccctagcgat aaacgacgct
    45601 ggccgtcgat tggtcgggcg gggttttgct acgaagcgga gatccgcgac gatcacaatc
    45661 gcccgctccc ggcaggtgag atcggtgaaa tctgtattaa aggcgtacca gggaaaacca
    45721 tcttcaaaga gtattttctc aacccgaaag ccactgcgaa agtgctggaa gccgacggct
    45781 ggctacatac cggcgatacc ggataccgcg acgaagaagg ctttttttat ttcgtcgatc
    45841 gccgctgcaa tatgatcaaa cgcggtggcg agaatgtctc ctgcgtggag ctggaaaata
    45901 tcatcgccac ccatccgaaa attcaggaca tcgtggttgt gggtattaaa gattcgattc
    45961 gcgatgaagc catcaaagca tttgtggtgc tgaatgaagg tgaaacattg agcgaagagg
    46021 aattttttcg cttctgcgaa caaaatatgg cgaaatttaa agtgccctct tatctggaga
    46081 tcagaaaaga tctgccacgt aattgctcgg ggaaaataat tagaaagaat ctgaaataaa
    46141 tgaaacgtca gggaactacc ctgccagcta ataatcacgc cctaaaacaa tatgcatttt
    46201 ttgcagggat gttgtcgtcc ctaaaaaagc aaaaatggag aaaaggaatg agtgaatcat
    46261 tacatctgac ccgcaatgga tcaattctgg aaattaccct tgatcgccca aaagcgaatg
    46321 ctattgatgc aaaaaccagc tttgaaatgg gcgaagtatt tctaaatttc cgtgacgatc
    46381 cgcaattacg tgtcgccatt attaccggtg ccggagagaa attcttttcc gcgggctggg
    46441 atttaaaagc ggcagcagaa ggcgaagcac cggatgctga ctttggcccg ggtggttttg
    46501 cgggattaac cgaaattttc aatctcgaca aaccagttat cgcagctgtg aacggctacg
    46561 cctttggcgg cggtttcgaa ctggcgctgg cggcagattt tattgtttgt gccgataacg
    46621 ccagcttcgc cctgccggaa gccaaactgg gcatcgttcc tgacagcggc ggtgtgctgc
    46681 gtctgccgaa gatcctgccg cctaccatcg tcaatgaaat ggtgatgacc ggcagacgaa
    46741 tgggcgcaga agaggcgctg cgttggggga tagtcaaccg cgtggttagc caggcggaac
    46801 tgatggataa cgcccgcgaa ctggctcagc agctggttaa cagcgccccg ctggcgattg
    46861 cggcgctgaa agagatctac cgcaccacca gcgaaatgcc ggtagaagaa gcgtatcgct
    46921 atattcgcag cggcgtgttg aaacactatc catcggttct gcattcggaa gatgccattg
    46981 aagggccgct ggcgtttgcc gagaagcgcg atccggtgtg gaaaggacgt taacaaccgt
    47041 gagctattac gcctttgagg ggttaattcc ggtggttcac ccgacggcgt ttgtccatcc
    47101 cagtgccgtc ttgattggcg atgtgattgt gggagccggt gtctacatcg gcccactcgc
    47161 ctcactgcgt ggtgactacg ggcggttgat cgtgcaagcg ggagccaata ttcaggatgg
    47221 ctgcattatg catggctact gcgacactga caccatcgtt ggggaaaacg gccatatcgg
    47281 gcatggagcg atcctgcatg gttgtgtgat tggtcgcgat gcattggtcg ggatgaacag
    47341 cgtaattatg gatggcgcgg tcattggcga agagagcatt gttgccgcaa tgagctttgt
    47401 caaagcaggc tttagtggcg agaaacgcca gttgttgatg ggtacgccag cccgcgctgt
    47461 acgcagtgtt agtgacgacg agttacactg gaaacggttg aataccaaag agtatcagga
    47521 tcttgtcggg cgctgtcatg catcgttaca tgaaacgcag ccactgaggc aaatggagga
    47581 aaatcgcccc cgtttgcagg ggacgacgga tgtgacgcct aaacggtgat cttgccggat
    47641 gcgacctggt tgctggataa gatgctacaa catcgtatct ggcaagcagg acgcccggta
    47701 tcaacgagat aaaattaacg acgcatactc tttgacaaca tcatctgcca cttctgctcg
    47761 cggttaagtt ccgtgagcgg aacggcagga atacgcggtg tctttcttac gccgactaat
    47821 ttttcccgac tgttattgcg caggcgcgcg taaatttgct catcgatatc gaccacttta
    47881 accagtcgct gacactggca tccccgccct tccagcttat tagggatcat cttaacttcg
    47941 cagcttattt ctgtgacttc cgacagaata taagtcaggg tattaaccgc cttgctgtgt
    48001 tcaatatcga aatgaataga gatctctctt gctatcaccc accgattttc agccatcatc
    48061 cattcggcga ttaacaagta gagtggtttt tcaacatatc cttcacacat acgaactcca
    48121 gtttcacaaa ttaaaagtct agctatgact cgttttgaag caattaaaca aggccatatt
    48181 aaaattgtgg atatatcgat agtatgcaac ttcaccgtcg ataagtgtga gctaaatcct
    48241 gcttatgtca tcaaaaatat cgattcccca aaagatttac ttaatgggca aaaaaaaacg
    48301 gtcctcatca gagaaccgta ttagttattc gatcaaatta gcggatgaaa aatatccgcc
    48361 acgacacgct attatttgat ctgtgcgtgc atttcctgca ccgaaattac tttttcagtc
    48421 gcatcggcat tcagcgccat cgcggtagcg aaaccaccgt tcagggtggt gtcataatgc
    48481 actttatatt gcagcgcact gcgacggatc acgcgggagt cttcaatcgc acgacggcct
    48541 gaggtggtgt tgatgatgta ggtatattcg ccattcttga tacggtcctg aatgtgcgga
    48601 cggccttcat gcaccttgtt taccagacgc ggattgatac ccgcttcgcc cagcacaatc
    48661 gccgtgccgt gggtcgcatc cagctcgaag ccctgtttca gcagttttgc cgccaggtcc
    48721 accacgcgtt ctttatcgcc ttcgcgcacg gaaagcagcg cacgaccgtg tttcttcatg
    48781 gtggagttgc tgcccagctg cgctttggcg aacgcttcag cgaaggtgcg gcccacgccc
    48841 atgacttccc cggtagagcg catttctggc cctaacagcg ggtcaacgcc tgggaattta
    48901 ttgaacggca gcaccacttc tttcaccgag tagtacggcg gaataacttc tttggttacg
    48961 ccctgctcag ccagcgattt gccagccatc acacgcgccg ccacttttgc cagcggtacg
    49021 ccggtggctt tggagacgaa cggaacagta cgcgccgcac gcgggtttac ttcaatcagg
    49081 taaacttcgt tgtttttcac cgcaaactgc acgttcatca gaccgcgcac ctgcaattcg
    49141 aaggccagtt tctgcacctg ctggcgcatc acatcctgaa tttcctgact taaggtgtac
    49201 gctggcagcg aacatgcgga gtcaccggag tgcacacccg cctgctcgat gtgctccatg
    49261 atgccgccaa tcagcaccat ttcgccgtcg cagatggcat ccacgtcaac ttctactgcg
    49321 tcatcaagga aatgatccag caacactggc gcatcgttag acacgctaac cgccgtctgg
    49381 aagtagcgac gcaggtcagc ttcgtcatag acgatttcca tcgcccgacc gccgagaacg
    49441 taagacggac gcaccaccag cgggtagcca atctcttttg ccttctcaac tgccatttca
    49501 atagcagtaa cggtggcgtt cgccggttgt ttcagtttca gacggtcaac cgcatgctgg
    49561 aagcgttcac ggtcttccgc acggtcgata gcatccgggc tggtgccgat aaccggtacg
    49621 ccagcggctt ccagtgcacg cgccagtttc agcggggtct gaccgccgta ctgaacgata
    49681 acgcctttcg gcttctcaat acgcacgatt tccagcacat cttccagagt taccggctcg
    49741 aagtagaggc gatcggaagt gtcgtagtcg gtagagacgg tttccgggtt acagttaacc
    49801 ataatggttt cgtaaccgtc ttcacgcagc gccagcgagg cgtgtacgca gcagtagtcg
    49861 aattcgatac cctgaccgat acggtttgga ccgccgccaa gcaccatgat tttttcacgg
    49921 tcggtagacg gattcgcttc gcactcttct tcataagtgg agtacatgta agcggtgtcg
    49981 gtggcaaact ctgccgcaca ggtatccacg cgcttgtaga ccgggtgcag gtcatattgg
    50041 tcacgcagct tacggatttc cgcttcgcgt acgcccgcca gttttgccaa gcgcgcatcg
    50101 gcaaagcctt tgcgtttcag ctggcgcagg aattcagcgt tcaggccagt gatgcccact
    50161 tccgccactt tctcttccag acgcaccagt tcttcaatct gtaccaggaa ccagcggtca
    50221 atgttagtca ggttgaagac gccgtccaca gacaggcccg cgcggaaagc atcggcgatg
    50281 taccagatac gctctgcgcc tgcgtctttc agttcgcgac ggattttggt taacgcttcc
    50341 gggtcatcca ggctcacttt cgggtcgaag ccagttgcgc ccacttccag gccgcgcagc
    50401 gctttttgca gggattcctg ctgcgtgcga ccaatcgcca tcacttcgcc aaccgatttc
    50461 atctgagtgg tcagacggtc gttagcaccg gcgaattttt cgaagttgaa gcgaggaatt
    50521 ttagtaacca catagtcgat ggacggctcg aaggaggccg gagtacgtcc gccagtgatg
    50581 tcgttcatca gttcgtctag ggtgtaaccc accgccagtt tcgccgccac tttagcaatc
    50641 gggaaaccgg tcgctttcga cgccagcgcc gaagaacggg acacgcgtgg gttcatttcg
    50701 ataacaatca ggcgaccgtt tttcgggttc accgcaaact ggacattgga accaccggtt
    50761 tcaacgccga tttcacgcag caccgccatc gaggcgttac gcatgatttg atattctttg
    50821 tcggtcagcg tttgggctgg cgcgacagtg atggagtcac cggtgtggat gcccatcgca
    50881 tcaaagtttt cgatagagca gacgatgatg cagttgtcgt ttttatcacg caccacttcc
    50941 atctcgtact ctttccagcc gatcagcgac tcatcaatca gcaactcttt ggtcggagag
    51001 agatccagac cgcgggcgca aatttcttca aactcttcac ggttataagc gataccgccg
    51061 ccgctaccgc ccatggtaaa ggatgggcga ataatgcacg ggaagcccac gtcagcggca
    51121 acagccagcg cttcttccat cgtgtgtgcg ataccggaac gcgcggtttc cagaccaatt
    51181 ttcttcatcg ctacgtcgaa acgacggcgg tcttctgctt tatcaatcgc atcggcagtg
    51241 gcaccaatca tggtgacacc gaactcttcc aacacgccct gacgttccag ctccagcgcg
    51301 cagttcagcg ccgtctgacc gcccatggtt ggcagcaccg cgtccgggcg ctctttttca
    51361 ataatcttgc gtaccacttc ccagtgaatc ggctcgatgt aggtcgcatc ggccatttcc
    51421 gggtcggtca tgatggtcgc cgggttggag ttcaccagaa tgacgcggta accctcttcg
    51481 cgcagggctt tacatgcttg cgcgccagag tagtcaaact cacacgcctg accgataaca
    51541 atcgggcccg cacccagaat caggatactt tttatatctg tacgttttgg catggctctt
    51601 ttactcctga ttacttagcg gttttacggt actgctcaat taacttgata aagtggtcga
    51661 acaacggcgc ggcgtcgtgt ggaccagggc tggcttcagg gtgaccctgg aagctgaacg
    51721 ccggcttatc gatgcgatga atgccctgta acgtaccgtc gaacagggat ttatgcgtga
    51781 cacgcaggtt tgcaggtaat gttgcttcat ccaccgcaaa accgtggttc tgggcggtga
    51841 tcatcaccac gtttttctcc acatctttaa ccggatggtt gccgccgtgg tgaccaaatt
    51901 tcattttgac agtcttcgca ccgctcgcca gcgccagcag ctgatgaccg agacagatgc
    51961 cgaataccgg aatatcggtt tcgaggaact tctggatggc ggtaatggcg taatcgcacg
    52021 gggccgggtc gccaggaccg ttggagagga agatgccgtc tggattcatt ttcagcacat
    52081 cttccgcaga agtttgcgcc ggaacgatgg tcaggcgaca gcctctatcc accagcatcc
    52141 gcaggatgtt gcgcttggca ccaaaatcat aagccacgac gtggaacggc agctcgtctt
    52201 cttttttcgc ttctggcagg ccgccggtca acgtccagct cccttgtgtc cagctatagg
    52261 cttctgcggt ggtcacttct tttgccagat ccatgccgtt cagacctggg aacgcgcggg
    52321 ctttttctaa cgccagcgcc gcatccgggt tatcgcctgc gataatgcag ccattctgtg
    52381 cgcctttctc gcgcagtaaa cgcgtcagct tacgggtatc gatatcggca atcgccacga
    52441 tgttatggcg cttcaggtaa gaagagaggt cttcggtatt acggaagttg ctggcaatca
    52501 gcggcaggtc gcgaatcacc agaccttgtg catgtacctg agaagattct tcatcggcgt
    52561 cattagtgcc gacattgcca atatggggat aagtaagagt aacgatttga cgagaatagg
    52621 aaggatcagt gaggatttct tgataaccgg tcattgaagt attgaaaacg acttccccaa
    52681 ccgccgaacc tgttgcccct atggcccgac cgtgaaactg ggttccgtct tccagaacca
    52741 atagcgctga cttaatcaaa acaccctcca gagaatactc actcacttta tttgcatatt
    52801 aattcacagt gatgacgtaa atcaatgcaa atctgcttac cgacgaattt ctggcaaacg
    52861 gcggcattct ggagatatgg gcgctaaaag tcaacttaat ggcaggattt tttatattat
    52921 tttgtggctc atgcatgttt tacaggcatt aagcgacaaa aacatcatct aacctgttct
    52981 ggaaaacgct tgcgcagcaa gaagattgca tgaaataaaa acgagcagga aaaagtggac
    53041 caaatggtca aaatttacat taaaacaaca aaaatagaag
//
LOCUS       DAZSIO010000008        51637 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZSIO010000008 DAZSIO010000000
VERSION     DAZSIO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 51637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51637
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.008"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            29..2419
                     /gene="gcd"
                     /locus_tag="ACHX5T_000398"
     CDS             29..2419
                     /gene="gcd"
                     /locus_tag="ACHX5T_000398"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR8465741.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(2466..4016)
                     /gene="cueO"
                     /locus_tag="ACHX5T_000399"
     CDS             complement(2466..4016)
                     /gene="cueO"
                     /locus_tag="ACHX5T_000399"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR8465742.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMVIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            4182..4529
                     /locus_tag="ACHX5T_000400"
     CDS             4182..4529
                     /locus_tag="ACHX5T_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR8465743.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            4635..5501
                     /gene="speE"
                     /locus_tag="ACHX5T_000401"
     CDS             4635..5501
                     /gene="speE"
                     /locus_tag="ACHX5T_000401"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR8465744.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            5517..6311
                     /gene="speD"
                     /locus_tag="ACHX5T_000402"
     CDS             5517..6311
                     /gene="speD"
                     /locus_tag="ACHX5T_000402"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR8465745.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(6349..6711)
                     /gene="yacL"
                     /locus_tag="ACHX5T_000403"
     CDS             complement(6349..6711)
                     /gene="yacL"
                     /locus_tag="ACHX5T_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR8465746.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(6886..9483)
                     /gene="acnB"
                     /locus_tag="ACHX5T_000404"
     CDS             complement(6886..9483)
                     /gene="acnB"
                     /locus_tag="ACHX5T_000404"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR8465747.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            9838..11691
                     /locus_tag="ACHX5T_000405"
                     /pseudo
     CDS             9838..11691
                     /locus_tag="ACHX5T_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(11762..13186)
                     /gene="lpdA"
                     /locus_tag="ACHX5T_000406"
     CDS             complement(11762..13186)
                     /gene="lpdA"
                     /locus_tag="ACHX5T_000406"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR8465748.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(13511..15391)
                     /gene="aceF"
                     /locus_tag="ACHX5T_000407"
     CDS             complement(13511..15391)
                     /gene="aceF"
                     /locus_tag="ACHX5T_000407"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR8465749.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(15406..18069)
                     /gene="aceE"
                     /locus_tag="ACHX5T_000408"
     CDS             complement(15406..18069)
                     /gene="aceE"
                     /locus_tag="ACHX5T_000408"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR8465750.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(18230..18994)
                     /gene="pdhR"
                     /locus_tag="ACHX5T_000409"
     CDS             complement(18230..18994)
                     /gene="pdhR"
                     /locus_tag="ACHX5T_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR8465751.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            19535..20908
                     /gene="aroP"
                     /locus_tag="ACHX5T_000410"
     CDS             19535..20908
                     /gene="aroP"
                     /locus_tag="ACHX5T_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR8465752.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(20951..21805)
                     /gene="ampE"
                     /locus_tag="ACHX5T_000411"
     CDS             complement(20951..21805)
                     /gene="ampE"
                     /locus_tag="ACHX5T_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR8465753.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(21802..22353)
                     /gene="ampD"
                     /locus_tag="ACHX5T_000412"
     CDS             complement(21802..22353)
                     /gene="ampD"
                     /locus_tag="ACHX5T_000412"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR8465754.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            22441..23334
                     /gene="nadC"
                     /locus_tag="ACHX5T_000413"
     CDS             22441..23334
                     /gene="nadC"
                     /locus_tag="ACHX5T_000413"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR8465755.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            23537..23977
                     /gene="ppdD"
                     /locus_tag="ACHX5T_000414"
     CDS             23537..23977
                     /gene="ppdD"
                     /locus_tag="ACHX5T_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR8465756.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            23987..25134
                     /locus_tag="ACHX5T_000415"
                     /pseudo
     CDS             23987..25134
                     /locus_tag="ACHX5T_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
     gene            25124..26326
                     /gene="hofC"
                     /locus_tag="ACHX5T_000416"
     CDS             25124..26326
                     /gene="hofC"
                     /locus_tag="ACHX5T_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFR8465757.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(26361..27404)
                     /gene="guaC"
                     /locus_tag="ACHX5T_000417"
     CDS             complement(26361..27404)
                     /gene="guaC"
                     /locus_tag="ACHX5T_000417"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR8465758.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(27566..27604)
                     /locus_tag="ACHX5T_000418"
                     /pseudo
     CDS             complement(27566..27604)
                     /locus_tag="ACHX5T_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27629..28249
                     /gene="coaE"
                     /locus_tag="ACHX5T_000419"
     CDS             27629..28249
                     /gene="coaE"
                     /locus_tag="ACHX5T_000419"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR8465759.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            28249..28992
                     /gene="zapD"
                     /locus_tag="ACHX5T_000420"
     CDS             28249..28992
                     /gene="zapD"
                     /locus_tag="ACHX5T_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR8465760.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            29002..29199
                     /gene="yacG"
                     /locus_tag="ACHX5T_000421"
     CDS             29002..29199
                     /gene="yacG"
                     /locus_tag="ACHX5T_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR8465761.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(29299..29688)
                     /gene="mutT"
                     /locus_tag="ACHX5T_000422"
     CDS             complement(29299..29688)
                     /gene="mutT"
                     /locus_tag="ACHX5T_000422"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR8465762.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(29748..32453)
                     /gene="secA"
                     /locus_tag="ACHX5T_000423"
     CDS             complement(29748..32453)
                     /gene="secA"
                     /locus_tag="ACHX5T_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR8465763.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(32515..33027)
                     /gene="secM"
                     /locus_tag="ACHX5T_000424"
     CDS             complement(32515..33027)
                     /gene="secM"
                     /locus_tag="ACHX5T_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR8465764.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNPNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(33258..34175)
                     /gene="lpxC"
                     /locus_tag="ACHX5T_000425"
     CDS             complement(33258..34175)
                     /gene="lpxC"
                     /locus_tag="ACHX5T_000425"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR8465765.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(34276..35427)
                     /gene="ftsZ"
                     /locus_tag="ACHX5T_000426"
     CDS             complement(34276..35427)
                     /gene="ftsZ"
                     /locus_tag="ACHX5T_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR8465766.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(35488..36750)
                     /gene="ftsA"
                     /locus_tag="ACHX5T_000427"
     CDS             complement(35488..36750)
                     /gene="ftsA"
                     /locus_tag="ACHX5T_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR8465767.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(36747..37577)
                     /gene="ftsQ"
                     /locus_tag="ACHX5T_000428"
     CDS             complement(36747..37577)
                     /gene="ftsQ"
                     /locus_tag="ACHX5T_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR8465768.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(37579..38499)
                     /gene="ddlB"
                     /locus_tag="ACHX5T_000429"
     CDS             complement(37579..38499)
                     /gene="ddlB"
                     /locus_tag="ACHX5T_000429"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR8465769.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(38492..39967)
                     /gene="murC"
                     /locus_tag="ACHX5T_000430"
     CDS             complement(38492..39967)
                     /gene="murC"
                     /locus_tag="ACHX5T_000430"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR8465770.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(40021..41088)
                     /gene="murG"
                     /locus_tag="ACHX5T_000431"
     CDS             complement(40021..41088)
                     /gene="murG"
                     /locus_tag="ACHX5T_000431"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR8465771.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(41085..42329)
                     /gene="ftsW"
                     /locus_tag="ACHX5T_000432"
     CDS             complement(41085..42329)
                     /gene="ftsW"
                     /locus_tag="ACHX5T_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR8465772.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(42329..43645)
                     /gene="murD"
                     /locus_tag="ACHX5T_000433"
     CDS             complement(42329..43645)
                     /gene="murD"
                     /locus_tag="ACHX5T_000433"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR8465773.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(43648..44730)
                     /gene="mraY"
                     /locus_tag="ACHX5T_000434"
     CDS             complement(43648..44730)
                     /gene="mraY"
                     /locus_tag="ACHX5T_000434"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR8465774.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(44724..46082)
                     /gene="murF"
                     /locus_tag="ACHX5T_000435"
     CDS             complement(44724..46082)
                     /gene="murF"
                     /locus_tag="ACHX5T_000435"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR8465775.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(46079..47566)
                     /gene="murE"
                     /locus_tag="ACHX5T_000436"
     CDS             complement(46079..47566)
                     /gene="murE"
                     /locus_tag="ACHX5T_000436"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR8465776.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(47553..49319)
                     /gene="ftsI"
                     /locus_tag="ACHX5T_000437"
     CDS             complement(47553..49319)
                     /gene="ftsI"
                     /locus_tag="ACHX5T_000437"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR8465777.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(49335..49700)
                     /gene="ftsL"
                     /locus_tag="ACHX5T_000438"
     CDS             complement(49335..49700)
                     /gene="ftsL"
                     /locus_tag="ACHX5T_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR8465778.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(49697..50638)
                     /gene="rsmH"
                     /locus_tag="ACHX5T_000439"
     CDS             complement(49697..50638)
                     /gene="rsmH"
                     /locus_tag="ACHX5T_000439"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR8465779.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(50640..51098)
                     /gene="mraZ"
                     /locus_tag="ACHX5T_000440"
     CDS             complement(50640..51098)
                     /gene="mraZ"
                     /locus_tag="ACHX5T_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR8465780.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        12164 a        14428 c        12795 g        12250 t
ORIGIN      
        1 tattaatgaa ttgaaatggt gtctctttat ggcaattaac aatacagcct cgcgacgatt
       61 actcgtcacg ctaacagccc tttttgcagc gctttgcggg ctgtatctac tcattggcgg
      121 aggctggctg gtcgcgattg gcggctcctg gtactaccct atcgctggcc ttgtgatgct
      181 cggcgtcgcc tggatgctgt ggcgcagtaa acgcgccgcg ctttggctat acgcagccct
      241 gctgctcggc accatgattt ggggcgtctg ggaagttggt ttcgacttct gggcgctgac
      301 tccgcgcagc gatattctgg tcttcttcgg catctggctg atcctgccgt ttgtctggcg
      361 tcgcctggtc attcctgcca gcggcgcagt tgccgcactg gtggtcgcac tgctgattag
      421 cggtggtatc ctgacctggg ccggatttaa cgatccgcag gagatcaacg gcaccttaag
      481 cgccgatgcc acacctgccg aagctatctc ccccgttgcc gatcaggact ggcctgcgta
      541 tggccgcaat caggaaggtc aacgcttttc accactgaag caaattcacg ccgataacgt
      601 ccacaagctg aaagaagcct gggtgttccg tactggcgat gtgaagcagc cgaacgatcc
      661 gggtgaaatc accaatgaag tgacgccgat taaagtgggc gatacccttt acctgtgtac
      721 cgctcaccag cgcctgtttg cgcttgatgc cgccagcggc aaagagaaat ggcattacga
      781 tcctgagctg aaaaccaacg agtctttcca gcacgtaacc tgccgtggcg tctcttatca
      841 tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg gattgcccgc gtcgtatcat
      901 tcttccagtg aatgatggtc gcctgattgc gattaacgct gaaaacggca aactgtgcga
      961 aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat atgccagaca ccaaaccggg
     1021 tctgtatgag ccgacttctc cgccaattat caccgataaa accatcgtga tggccggttc
     1081 agtcaccgat aacttctcaa cccgcgaaac gtctggcgtg atccgtggtt ttgatgtcaa
     1141 caccggggag ctgctgtggg cttttgatcc gggcgcgaaa gatccgaacg caatcccgtc
     1201 agacgaacac acctttacct ttaactcacc aaactcgtgg gcaccagcgg cctatgacgc
     1261 gaagctggat ctggtctatc tgccgatggg cgtgaccacg ccggatatct ggggcggtaa
     1321 ccgcacaccg gaacaggaac gttatgccag ctcgattctg gcgctgaatg ccactaccgg
     1381 gaaactggcg tggagctacc agaccgttca ccacgacctg tgggacatgg atcttccggc
     1441 acagccgacg ctggcggaca tcaccgttaa tggtcagaaa gtgccgatta tttacgctcc
     1501 ggcgaaaacc ggtaacattt ttgtgctcga tcgtcgtaat ggcgaactgg tggttccggc
     1561 cccggaaaaa ccggttcccc aaggtgctgc caaaggcgat tacgttaccc ctactcaacc
     1621 attctcggaa ctgagcttcc gtccgacgaa agatttgagc ggtgcggata tgtggggagc
     1681 caccatgttt gaccaactgg tgtgccgcgt gatgttccac cagatgcgct atgaaggcat
     1741 tttcacgccg ccatctgaac agggtacgct ggtcttcccg ggtaacctgg ggatgttcga
     1801 atggggcggg atttccgttg atccaaatcg tgaagtggca attgccaacc caatggcact
     1861 gccgtttgtt tcgaaactgc tcccgcgtgg tcctggcaac ccgatggagc agccgaaaga
     1921 tgccaaaggc acgggtacgg aatccggcat tcagccacag tacggtgtac cgtatggtgt
     1981 cacgctcaac ccgttcctct caccatttgg tctgccatgt aaacagccag catggggtta
     2041 tatctcggcg ctggatctga aaactaatga agtggtgtgg aagaaacgta ttggtacgcc
     2101 gcaggacagt atgccgttcc cgatgccggt tccggtgccg ttcaatatgg gtatgccgat
     2161 gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt atcgccgcta cggcagataa
     2221 ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg tggcagggtc gtttaccggc
     2281 gggtggtcag gcaacaccga tgacctatga agtgaatggc aagcagtacg tggtgatctc
     2341 cgcaggcggt cacggttcat ttggtacgaa gatgggcgac tatattgtgg cttatgccct
     2401 gccggatgat gtgaaataac cataatgccc ggagagatcc gggcatattt ccgaatacgg
     2461 tctttttata ccgtaaaccc taacatcatc cccgtatctt catgctccag cagatggcag
     2521 tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa acttcaccag cacttcactg
     2581 acattgcctt ctaccttaac ggtatctttc cagcccgcgc gatgagccgc tggcggtttg
     2641 ccattttctg acaagatacg gaactgcgtg ccgtgaatat ggaacggatg cagcatcatg
     2701 tcgcccacgc cagagataac ccaacgttcg tactgccctt tcgccgccgc aaacatcggc
     2761 ttattcatat caaacgcctg accgttgatt ttattggcat ggtggaaatc gaacttcccg
     2821 ccgtggttca tatgattcat attgccgtgc cccatatggc ccatcatctg gctgtgatcc
     2881 attccggcca tcgcctgatc gccatatttc tccattagca tctgcatccc catcatatcg
     2941 agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca gcccttccag cgaaggtaac
     3001 gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg cactaatagc aatcggctga
     3061 atccgcatta ccggatgcgg cttgtcaaac ggtgcaatca ccatccccat ctggctgacc
     3121 ggcagcgtca ccaggtcaaa gggtttgttg tcgttaacct ccaccagcac ttcaaaacgc
     3181 tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt caggtagcag accaccgtcg
     3241 ctggcaatca catacagcgg gcgattgtcg ctggtggcga aattgagcga gcgggcatta
     3301 cagccattga gcaaacgcag gcgcagccaa ccacgcgggg cagcgtgttg tgggtagatt
     3361 gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg cggcggtcat cacatccagt
     3421 tgataatcaa tctgcccgtc ggcgctaaat ttcttatcct gaacgatcac cggaacatca
     3481 tcgatacccc actgttttgg cagcattaat ttcaggatct cgtcatcttc aatcaccacc
     3541 agaccagcca gccccatcgc cacctgtcgc ccggtcttgc catgttgatg tggatggaac
     3601 cagcaggtag cggcaggttg atcaacgttc aacgtcaccg agcgcttgcc acctggcgga
     3661 ataattccct gtgggccgcc gtcgacgtca cccggtactt ccagcccgtg ccagtgcaac
     3721 gtcgtctctt ccgccagttg gttgtagata tcaaccgtta ccgctttgcc gcgctgtaat
     3781 ttcaccgccg gccccagcag attgccgtta tagccccagg tagttgcagt tttctcgcca
     3841 aaggtggact gacctgcgcc aatagttaac tgaatgcgat tacgggcatc ggtcgtgagc
     3901 aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga ctgcacggct ccacagtggc
     3961 aaggctgaag ccacacccag tgcgacggaa tatttcaaga aatcacgacg ttgcatagtt
     4021 atttccttat tcttaagcag gcgaaacaaa atcaaacgtt gagcatagtc cttcccctta
     4081 cgggaaggtc aagccgcaga cacaatttaa tcgacaataa tggtcgtcgc ctcgctcgca
     4141 gtgtgctaac gtttatcttc tttaagcccc tggtagaagc aatgaagacg tttttcagaa
     4201 cagtgttatt cggcagcctg atcgccgttt gcgcaaacag ttacgcgctc agcgagtctg
     4261 aagccgaaga tatggccgat ttaacggcag tttttgtctt tctgaagaac gattgtggtt
     4321 accagaactt atctaacggg caaattcgtc gcgcactggt ctttttcgcc cagcaaaacc
     4381 agtgggatct cagtaattac gacaccttcg acatgaaagc tctcggtgaa gacagctacc
     4441 gcgatctcag cggcattggc attcccgtcg ctaaaaaatg caaagccctg gcccgcgatt
     4501 ccttaagcct gcttgcctac gtcaaataat ccctgatacc tttttgtaga aatattgacc
     4561 gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt tttttacggg tgttaacaaa
     4621 ggaggtatca acccatggcc gaaaaaaaac agtggcatga aacgctacac gaccagtttg
     4681 ggcagtactt tgcggtagat aacgttctgt atcatgaaaa gaccgatcac caggatctga
     4741 tcatttttga gaacgctgca tttggtcgcg taatggcgct ggatggcgta gtacaaacca
     4801 ccgagcgcga cgagtttatc tatcatgaga tgatgaccca tgttccgcta ctggcccacg
     4861 gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg tgccatgctg cgtgaagtaa
     4921 cccgacataa aaacgttgag tcaatcacga tggtggaaat cgatgcgggt gtcgtgtcgt
     4981 tctgccgtca gtatctgccc aaccataacg ccggtagcta cgacgatccg cgctttcaac
     5041 tagtgatcga cgatggcgtc aatttcgtta atcaaaccag ccagaccttt gatgtcatta
     5101 tctccgactg caccgatcct atcggtcccg gcgaaagcct gttcacctca gcattttatg
     5161 aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt cgcacaaaac ggcgtctgct
     5221 ttttacagca ggaagaagcc atcgacagcc atcgcaaact cagccattac ttcagcgacg
     5281 ttggctttta tcaggcggcg atcccgacct attacggcgg tatcatgact tttgcatggg
     5341 cgacagataa cgacgcctta cgccatctct caaccgaaat tattcaggcg cgttttctcg
     5401 cttctggcct gaaatgccgt tattacaatc cggcaatcca tacggcagct tttgccttac
     5461 ctcagtatct gcaagacgca ctggcttcac agccgtccta aggagaagat aagaaattga
     5521 aaaaactgaa actgcatggc tttaataatc tgaccaaaag tctgagtttt tgtatttacg
     5581 atatctgcta cgccaaaact gccgaagagc gcgacggtta tattgcttat atcgatgaac
     5641 tctataatgc caaccgtctg accgaaatcc tgtcagaaac ctgttccatt atcggggcca
     5701 atattcttaa catcgcccgc caggattacg aaccacaggg tgccagcgtc actattctgg
     5761 tgagtgaaga accggttgac ccgaaactca tcgacaaaac agaacacccc ggcccactgc
     5821 cagaaacggt ggttgctcat ctcgataaaa gtcatatttg cgtacatacc tacccggaaa
     5881 gtcatcctga aggcggttta tgtaccttcc gcgccgatat tgaagtctct acctgcggcg
     5941 tgatttctcc gctgaaggcg ctgaattacc tgatccacca gcttgagtcc gatatcgtaa
     6001 ccattgatta tcgcgtgcgc ggttttactc gcgacattaa cggtatgaag cactttatcg
     6061 accatgagat taattcgatt cagaacttta tgtctgacga tatgaaggcg ctgtatgaca
     6121 tggtggatgt aaacgtctat caggaaaata tcttccatac caagatgttg cttaaagagt
     6181 tcgaccttaa gcactacatg ttccacacca aaccggaaga tttaaccgac agcgagcgcc
     6241 aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat ttattacggg cgcaatatgc
     6301 cagctgttta acggctctgg cggagctccc aggctccgcc agatttattt acttctgctg
     6361 cacgaaattg cggtaagccg ccacgacctg cagaaaatcc tcaacgccgc atagcgacag
     6421 gctttcttcg tcgtagtagt tcatcccctc ttccatttca tcgccagcga attccagttg
     6481 attggcgcga accatcacct cttcagcgtc catccacagg gtgtattcat gccctgcccg
     6541 ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct tgttccactt catcaagcaa
     6601 ggccaggttt tctttcacct cttcattaaa ccagtgcccg accacttcat gccccatgga
     6661 catacgcacc tttaccactc cggtaatatc gcgcagaaat tcgtaatcca tagtgtgttc
     6721 ctctgctccc ggcaatgcgc cgaaatcgca tcgcgccatt gatgtaatta tcgcagcctt
     6781 accggagaaa aacagcggaa cggcgatagt ttagaaataa aaaatgcccg ggaatctcat
     6841 tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt acttattaca ccgcagtctg
     6901 gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc tggttgaagt tcagataacg
     6961 ataagtatca acggcagttt tatctacttg cgcaacgtag gtctggtact cttccggcgt
     7021 cggcagtttg ccaatcagcg ccgcaacagc cgccagttct gcagaagcca ggaagacatt
     7081 cgcgccagta cccaaacggt tcgggaagtt acgggtagag gtggaaacca ccgtcgcacc
     7141 gtctgctaca cgcgcctggt tacccataca cagggaacag cccgggatct cgatacgagc
     7201 cccgctctta ccgaagacgc tgtagtagcc ttcttccgtc agttgcgcgg cgtccatacg
     7261 ggttggcggt gccacccaca ggcgggtcgg caactgacct ttgtgcgcat ccagcagttt
     7321 accagcggca cggaagtgac cgatgttagt catgcaagaa ccgataaata cttcgtcgat
     7381 cttctcgccc tgtaccgcag acagcggacg tgcgtcatcc ggatcgttcg gagcacacag
     7441 gattggctct ttaatctccg ccagatcgat gtcgatcact gccgcgtatt ccgcatctgc
     7501 atcggcttcc agcagctcag gattcgccag ccatttttcc atgccctgaa tacggcgttc
     7561 cagggtacga cgatcgccgt aaccttccgc gatcatccac ttcagcagga cgatgttgga
     7621 gctcaggtat tcgatgatcg gttctttgtt cagcttgata gtacaaccag cggcagaacg
     7681 ctcggcggac gcatcggtta gctcaaaggc ctgctcaact ttcagatccg gcagaccttc
     7741 aatttccaga atgcgaccag agaagatgtt tttcttgcct ttcttctcaa cggtcagcag
     7801 accttgtttg atcgcataca gcggaatggc gtgtaccaga tcgcgcaggg tgatgcccgg
     7861 ctgcattttg cctttgaagc gcaccagaac ggattccggc atatccagcg gcattacgcc
     7921 agttgcggca gcaaacgcca ccagaccaga acccgccggg aaagagatac cgatcgggaa
     7981 acgggtatgg gagtcaccac cggtaccgac ggtatccggc agcagcatac ggttcagcca
     8041 ggagtgaatg acgccgtcac ccggacgcag cgacacaccg ccacggttca taatgaagtc
     8101 cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga tacgccgcgg tgtggcagaa
     8161 agactgcatc accaggtcag ccgagaagcc caggcacgcc aggtctttca gttcatcacg
     8221 ggtcatcggg ccggtggtgt cctgagagcc tacagaagtc attttcggtt cgcagtacgc
     8281 gcccggacga atgcctttca cgccacaggc acggcctacc attttctgcg ccagcgagaa
     8341 gccgcgatcg ctctcagcga catctttcgc ctgacggaac acatcactgt gcggcagacc
     8401 aagtgcttca cgcgctttgg tggtcaggcc acgcccgata atcagcggaa tacggccgcc
     8461 agcacgcact tcatcaatca gcacgtcggt tttcagttcg aaggtcgcca gcagttcgcc
     8521 tgtttcgtgg ttacgcactt cacctttgta cgggtaaacg tcaatcacgt cgcccatgtt
     8581 caggttagag acgttgactt cgattggcag tgcacccgcg tcttccatcg tgttaaagaa
     8641 gatgggtgca attttaccgc cgaggcacaa accaccgccg cgtttgttcg gcacatgtgg
     8701 aatatcatcg cccataaacc acagaacgga gttcgtggcg gatttacgcg atgaacccgt
     8761 acccacaacg tcaccgacgt acgccagcgg gaaacctttc tgttgcagag cttcaatctg
     8821 tttgatcggg ccaacaacac ctggctggtc tggctcaata ccttcacggg cgtttttcag
     8881 catcgccagc gcgtgcagtg ggatatccgg gcgtgaccac gcatccggtg ccggagaaag
     8941 gtcatcggtg ttggtttcgc cagtgacttt aaagacggta acggtcagtt tttcagccag
     9001 cgccgggcga ttcaggaacc attcggcatc cgcccaggac tgcattacct gcttcgcata
     9061 ttcgttgcct gctttcgctt tctcttctac gtcatagaag ttatcgaaca tcagcagcgt
     9121 gtgagaaagt gctttggcag caataggtgc cagtttggca tcatccagcg cgtcgatcag
     9181 cggatgaatg ttgtaaccac cctgcatggt gcccagcagt tcgatagctt tttccggagt
     9241 cagcagaggg gatttagctt cgcctttagc gatagcagcc aggaagcctg cttttacata
     9301 ggcggcttca tcgacgcctg ggggaacacg gttggttaac agatctaaca ggaattcttc
     9361 ttcgcccgcg ggcgggtttt tcagcagctc tacaagcgcg gccatttggt ttgcatccag
     9421 gggtttgggc gcaatcccct cagcggcacg ctcagctacg tgcttacggt attcttctag
     9481 cacgacggtt ctcctcgctc tcattgtcat agtgcggcag gcgattctct tcacgctcct
     9541 gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa cgggcagaat agcaggattt
     9601 tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa aagtggctga atcgttaagg
     9661 atggtctaga gattgtttcc ctccgtaaaa acttcgttaa ttcgcatggt gataatcacc
     9721 tttctcaacg aatcccaaga ttagtcaaaa tttaaactac cgcctcttta tactcggatt
     9781 cacagcacct gcgggtggca gttcgccgac cattgcgatt tccttgagat ccgaattatg
     9841 aaaatgactt tgccgtttaa accccatgtg ctggcgctaa tttgcagtgc cgggctttgt
     9901 gctgcctctg ccgggctata tataaaaagc cgcacagtgg aagcgcctgt agaaccgcaa
     9961 tcgacacaac tggctgtgtc tgacgctgcc gctgttacgc ttcctgcaac ggtttccgca
    10021 cctcccgtaa tacccgcagt cgtcaaatcc gcattcagca ctgcacaaat agatcaatgg
    10081 gtcgcacccg tcgcgctgta tcccgacgcc ctactttcgc aggtgctgat ggcatcaacc
    10141 tatccgacaa acgttgctca agcagtgcaa tggtcgcacg ataatccact taaacaaggc
    10201 gatgctgcta ttcaggcggt atctgaccag ccgtgggacg ccagcgttaa atcactggtg
    10261 gcctttccac aattgatggc attgatgggc gaaaacccgc aatgggtgca aaacctgggc
    10321 gatgcttttc tggcgcagcc gcaggacgtg atggactcgg tacagcgatt gcggcaactg
    10381 gcgcaacaaa ccggctcgct gaagtcatca accgaacaga aagttattac cacaacgaag
    10441 aaagctgtac cggtaaaaca gacagtcacg gcacccgtca taccatccaa taccgtttta
    10501 actgccaacc ccgtcattac agagccggca acaaccgtca tttccattga gcccgccaat
    10561 cctgatgtgg tctatattcc caactacaac ccaaccgtgg tttacgggaa ctgggccaat
    10621 actgcgtatc cgccggttta tctgccacca ccagccggag aaccgtttgt tgacagcttt
    10681 gtgcgcggat tcggctatag catgggcgtt gctaccacgt acgcactatt cagcagcatc
    10741 gactgagatg acgacgatca tgaccatcat catcatgacg atgatgatta tcatcaccac
    10801 gatggcggtc atcgtgacag taatggctgg caacacaacg gcgacaacat caatatcgac
    10861 gtcaacaatt tcaaccgtat caccggtgag catcttactg ataagaatat ggcatggcgg
    10921 cacaatccaa actaccgtaa tggtgtgccc tatcatgatc aggatatggc aaagcggttt
    10981 catcaaaccg atgtcaacgg cggaatgagt gccacgcagc tacctgctcc aacacgcgac
    11041 agccagcgtc aggcggcagc aagtcagttt cagcaacgaa cacacgccgc ccccgtcatt
    11101 acacgagata cccaacgtca ggcagcggca cagcggttta atgaagctga acactatggg
    11161 agctatgacg acttccgcga cttcagccgt cgccaaccac tgacccagca gcaaaaggac
    11221 gccgctcgtc agcgttatca gtcggcctct cctgagcagc gccaggcagt ccgcgagaaa
    11281 atgcagacta acccgcagat ccagcagcga agggaggcgg cgcgccagcg tattcagtcc
    11341 gcctcgcctg agcagcgcca ggcggttcgc gagaaaatgc agactaaccc gcagaaccag
    11401 cagcgaagag aggcagcgcg tgagcgtatt cagtctgcaa ctcccgaaca gcgccaggtg
    11461 tttaaggaaa aagtacagca gcgcccactg aaccaacagc aacgtgataa cgcccgccag
    11521 cgtatacaat cggcatcacc tgaacaacgt caggtttttc gggagaaagt tcaggagagc
    11581 cgcccacaac gtctaaacga cagtaaccat actgtcaggc tgaataacga gcaacggtca
    11641 gcagtacgcg aacgtctctc tgaacgtgga gcaagaagac tggaaaggta aattgcagac
    11701 gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg gatgttccgg caaacgaaaa
    11761 attacttctt cttcgctttc gggttcggca ggtcggtaat gctaccttcg aacacttctg
    11821 ccgccaggcc cacagactcg tgcagagtcg ggtgcgcgtg gatggtcagt gcgatgtctt
    11881 cagcatcaca acccatttcg attgccaggc cgatttcacc cagcagctcg ccgccgttgg
    11941 taccaacaat cgcaccaccg atcacacggt gagattcttt gtcgaaaatc agcttggtca
    12001 taccgtctgc gcagtcagaa gcgatagcac gaccagaagc agcccacggg aaggtggcgg
    12061 tttcatagct gatgcctttc tctttcgctt ctttctcagt cagacctacc catgcaactt
    12121 ctggttcggt ataggcgatg gacgggataa ctttcggatc gaagtagtgt ttcttaccgg
    12181 cgataacttc agcggcaacg tgaccttcgt gaacaccttt gtgtgccagc atcggctgac
    12241 cgacgatatc gccgatagca aagatgtgcg gtacgttggt acgcagctgt ttgtcaacgc
    12301 ggatgaaacc acggtcgtca acttccacgc cagctttgcc tgcgtcgagg tttttaccgt
    12361 tcggcacacg accaatcgct accagcacgg cgtcgtaacg ctgcggttca gctggtgctt
    12421 ttttgccttc catcgtcaca taaataccgt cttctttcgc ttcaacggcg gtaactttgg
    12481 tttccagcat caggttgaat ttcttgctga tacgcttggt gaagacttta acgatgtctt
    12541 tgtcagctgc cgggataacc tggtcgaaca tttcaaccac gtcaatctgt gaacccagcg
    12601 cgtggtaaac ggtgcccatt tccagaccga tgataccgcc acccattacc agcaggcgtt
    12661 ctggtacttc tttcagttcc agcgcgtcag tggagtccca gatacgcgga tcttcatgcg
    12721 gaataaacgg cagttggatc gggcgagaac ccgctgcaat gatcgcattg tcgaagttga
    12781 tcacggtttt gccgttctca ccttcaactt ccagggtgtt agccccggtg aatttaccca
    12841 gaccgttgac cactttgact ttgcggcctt tcgccatacc agccagacca ccggtcagct
    12901 gattgatcac tttctctttc caggtacgaa tcttgtcaat atcggttttc ggttcgccga
    12961 agacgatacc gtgttcagcc agcgctttgg cttcttcgat aacttttgct acgtgcagca
    13021 gtgctttaga agggatacag ccgacgttca ggcaaacacc gccaagggtg ttgtaacgtt
    13081 ctacgattac ggtttccaga cctaaatcag cgcaacggaa ggcagcggag taacctgcgg
    13141 ggcctgcccc aagtaccacg acctgagttt tgatttcagt actcatcatg acctctatat
    13201 atttatctcc ggcggtcata cccgtcgtct ttcaggccgc agcggcgtta gcttcacgcc
    13261 ctcaccccag tcacttactt atgtaagctc ctggggattc tggcgcttgc taccttgctg
    13321 cacccagaaa tccatagggt atgtgaagta ataccctaac caccaccggg tcgttctatc
    13381 cgtcggtatt ttacaaaatt gttaacaatt tttaaacaac aaacggcaac cgatttgtct
    13441 attcgctaat aacctcaata cattcatgag attaccagaa aaaagccggc cgttgggccg
    13501 gctcttttac ttacatcacc agacggcgaa tgtcagacag cgtgttgtta atgatggtaa
    13561 tgaaacgggc accatcagca ccgtcgatca cgcggtggtc gaaggagaga gaaatcggca
    13621 gcatcagacg cggcacgaac tctttaccat tccacaccgg ctccatcgcg gacttggaaa
    13681 cgccgaggat agccacttcc ggcgcgttca caatcggcgc gaagtgggta gtacccaggc
    13741 cgccgatgct ggagatggtg aagcaaccgc cctgcatttc gcccgcagtc agcttaccgt
    13801 cacgcgcttt cttagaaata gtcatcagct cgcgagacag ctcgatgatg cctttcttgt
    13861 tgacgtcttt gaataccgga acaaccagac cgttcggggt atccaccgcc acaccgatgt
    13921 tgatgtattt cttcagggtc agacgctgac cgtcttccga cagcgaacta ttgaagcgag
    13981 gcatctgctc aagagctgca gcaacggctt tcatgatgaa gacaaccggg gtgatcttca
    14041 catccagctt acgtttcgcc gcttcttcgt tctgctgttt acggaacgct tccagctcgg
    14101 tgatatcggt tttgtcgaag tgggtaacat gcgggatcat cacccagtta cggctcaggt
    14161 tagcaccaga aattttctgg atacggccca gttccacttc ttcgatttcg ccaaacttgc
    14221 tgaagtccac cttcggccac ggcagcatgc cagggatacc accgccagtc gccgccggag
    14281 ctgcttctgc acgtttgata gcttctttca cgtaagcctg aacgtcttcg cgcaggatac
    14341 gacctttacg gccagtgccc ttcactttcg ccaggttaac gccaaactcg cgtgccagac
    14401 ggcggatcag cggggtcgcg tgaacgtaag cgtcgttttc agcaaattca gatttgcctt
    14461 ccgctttcgc agccggagct tcagcttttg ctgccggagc cggcgctgcc gcttcctgtt
    14521 tcgcaggagc tgccgcaggc gctgcgcctt caacttcgaa gatcataatc agcgaaccag
    14581 ttttcacttt atcgccaacg ttgactttca gttccttcac gacgcctgca aacggagccg
    14641 gaacttccat agaagcttta tcgccttcta cggtaatcag tgactgttca gcggcaactt
    14701 tgtcgcccac tttcaccatc acttcggtca cttcaacttc gtcaccgccg atatccggaa
    14761 cgttaacttc tttcacgcca gctgctggtg caggggtcgc tgccggagtc gcttcctgtt
    14821 tagccgccgg agctgccgcg cctgcttcac ccgcgacttc gaagaccata atcagcgagc
    14881 cggtagacac tttgtcaccc acgttcactt tgatctcttt cacggtacct gcaaacggag
    14941 ccggaacttc catagaagcc ttgtcgcctt ctacggtgat cagcgactgt tcagcttcaa
    15001 ctttatcgcc aactttcacc aggatttcgg tcacttcaac ttcgtcgctg ccgatatccg
    15061 gaacgttaac gtcttttgcc gccgcagccg cgggtgctgc tgccggagct gcttctttct
    15121 tctcttctgc ctgagcaggt gcagcgtctg ctgcaccgtc ggcggaatcg aaaatcataa
    15181 tcagtgcgcc ggtctgggtt ttatcgccaa cagagacttt gatctcttta acgatacccg
    15241 cctgcggaga tggaacttcc atagaggctt tgtcgccttc tacggtgatc agcgactgtt
    15301 cggcttcaac tttgtcgccc actttgacca ggatctcggt gatttcaact tcatcagccc
    15361 cgatgtccgg tactttgatt tcgatagcca ttattctttt acctcttacg ccagacgcgg
    15421 gttaacttta tctgcatcga tgttgaattt ggcgattgcg tcagcaacca ctttcttatc
    15481 gatttcgcca cgtttagcca gttcgcccag cgccgcaacc acgacataag aagcatcaac
    15541 ttcgaagtgg tgacgcaggt tctcacggct gtcggaacga ccgaagccat cagtacccag
    15601 tacgcgatag tcgtcagccg gtacgtaagt acggacctgc tcagcgaaca gtttcatata
    15661 gtcggtagat gccactgccg gcgcgtcgtt catcacctga gcgatatacg gaacgcgcgg
    15721 agtttccagc ggatgcagca tgttccagcg ttcacaatcc tgaccatcac gcgccagctc
    15781 ggtgaaggag gtcacgctat aaacgtcaga acctacgccg taatctttcg ccaggatctc
    15841 tgccgcttca cggacgtgac gcaggatgga accggagccc agcagctgaa ctttaccttt
    15901 gctaccttca atggtttcga gtttgtagat acctttacgg ataccttcct cagcaccttc
    15961 cggcattgcc ggcatgtggt agttttcgtt cagcgtagtg atgtagtagt aaacgttctc
    16021 ttgtttttca ccgtacatac gctccagacc gtcatgcata atgacagcaa cttcgtaagc
    16081 gtaagccggg tcgtaagaga tacagttcgg gatagtcagc gactgaatgt ggctgtgacc
    16141 atcttcgtgc tgcagacctt caccgttcag ggtggtacga ccggaagtac cgccgatcag
    16201 gaagccacgc gcttgctggt ctccagccgc ccagcacaga tcgccgatac gctggaagcc
    16261 gaacatcgag taatagatgt agaatgggat catcggcaga ttgttcgtgc tgtaagaggt
    16321 cgccgctgcc agccaggaac accctgcgcc cagctcgttg atcccttcct gcagaatctg
    16381 acctttctcg tcttctttat agtaagcaac ctgctcgcgg tcctgcgggg tgtactgctg
    16441 accgttcggg ctgtaaatac caatctgacg gaacagacct tccataccga aagtacgcgc
    16501 ttcgtcggcg atgatcggta ccagacgatc tttgatcgac ttgttcttca gcatcacgtt
    16561 cagagcacga acgaaagcga tagtggtaga gagctctttg ctctgctctt ccagcagcgc
    16621 gccgaagtct tgcaggctcg gcagctcaag cttctcggtg aagttcggct gacggcttgg
    16681 cagataaccg tgcagtttct gacgctgagc gtgcagatag gtatgctctt cagaaccttc
    16741 cgggaaggtg atgtacggca gtttttcgat atctgcatca gacaccggca cattgaaacg
    16801 gtcgcggatg tgacgcacgc cgtccatgtt cattttctta acctggtgcg cgatgttttt
    16861 accttcagcc gcgtcgccca tgccgtaacc tttaatggta tgagcaagga ttactgtcgc
    16921 tttgcctttg gtttcctgcg ctttcttgaa tgcagcgtag attttcttcg gatcgtgacc
    16981 accacggttc agtgcccaga tctgctcgtc agtccagtct gcaaccagtg ctgcggtttc
    17041 aggatattta ccgaagaagt gttcacgaac gtacgcacca tctttcgatt tgaaggtctg
    17101 gtagtcgccg tcaacggttt cgttcatcag ctggatcagt ttaccgctgg tatctttacg
    17161 cagcagttca tcccaacggc taccccacat cactttgatc acgttccagc cagcaccttc
    17221 gaagatgcct tccagttcgt tgatgatctt gccgttaccg gtgaccgggc cgtcaagacg
    17281 ctgcaggtta cagttgataa cgaaaaccag gttatccagt ttttcacggg tagcgatggt
    17341 gatcgcacct ttggattccg gttcgtccat ttcaccgtcg ccgaggaacg cgtaaacggt
    17401 ctgtttagag gtatctttca ggccacggtg ttccagatat ttcaggaatt tagcctggta
    17461 aatagcacca atcggaccca gacccataga tacggtcggg aactgccaga attccggcat
    17521 cagtttcggg tgcggatagg aagagaggcc attgccgtga acttcctgac ggaagttatc
    17581 cagctgctcc tgagtcagac gaccttccag gaaagcacgg gcgtaaacgc ccggggagat
    17641 gtggccctgg aagtaaacca ggtcgccgcc atcctgctcg ttgcgtgcac ggaagaagtg
    17701 gttaaagcac acatcataaa tggttgcgga agactggaag gacgccatgt ggccgcccag
    17761 ttcgaggtct tttttcgacg cacgcagcac cgtcatgatg gcgttccagc ggatagctga
    17821 acgaatacgg cgttccagtt ccagattacc cggatactcc ggttgttctt caacggggat
    17881 ggtgttgatg tagttgctga tacctgtgcc tgcggctacg tttacaccgc ctttgcgggc
    17941 ttcagcaagc agttggtcga tcagatactg agcacgctca acaccttctt cacggatgac
    18001 cgattcgatc gcctggagcc agtcgcgagt ttcgatcgga tccacgtcat ttgggaaacg
    18061 ttctgacatg ggttattcct tatctatcta ataacgttga gttttctgga acctgtccca
    18121 ttgaactctc gccagaaagc tcaataagac aggttctacg tttagttacc gcgcttttat
    18181 atgcgcttga tttacaacat cttctggata atttttacca gaaaaatcac taattctttc
    18241 gttgctccag acgacgcaga gaacgctcac ggcggctctc ttcacggctt ctgtcgagca
    18301 aaatttcttc gataaaggcc agatggcgat gcgatgcttc gcgcgcttct tccggcttac
    18361 cgaccataat cgcttcaaat atgcgggtgc ggtgactact caccagcggc agcatctcgc
    18421 gacgcgaata gagcaattcg aagttctggc ggacattctg ggccaacatc ggctccatac
    18481 accttagcag atgaagcaga accacattgt gggccgcttc ggtgacggca atctgatact
    18541 ggagtacggc gtttgattcc gcgtccagat cgccagactg ctgcgccagc tctatggcgt
    18601 ggtggagttc acggatgcgt tccttgtctt catcggtact acgcagcgcg gcgtaataag
    18661 cggcgatacc ttccagggcg tgtcgtgttt cgagcaagtc atactgtgat tcaggatggt
    18721 cggagagcag ctccaccagc ggatcgctga agctttgcca taggctgctc tggacaaaag
    18781 tgccgccacc ctggcgacga agcaacaagc ccttcgcttc gagacgttga atcgcctcac
    18841 gcaaggaggg acgggagacg tcgaactgtt ttgccagttc gcgttccggt gggagttttt
    18901 cgcccgggcg gagagtgcct tcgaggatca aaaactccag ttgctgctca atcacatcgg
    18961 agagttttgg ttggcggatt ttgctgtagg ccatgagttc ctgtcttaag ccacttgccg
    19021 aagtcaattg gtcttaccaa tttcatgtct gtgacgctaa agtaacaaag tattcacctt
    19081 atgtccatac aggttttgat tgaaatcatg aaactgtgca cattttaaca acttgacata
    19141 tataacgttt caaagttgta actatgcaca aatgtagact ttacgtagga aagggatttt
    19201 ttaacgatta ttaaactata aaaatccgaa ttgaaccgat tcacttacca attttttgat
    19261 ttttaagtca ataaaaacga atttaaattc actctgcata ttgagagggg ttgaggctga
    19321 gctttacaaa cggtttcttt ttaagcaact catcttcaac catgcataaa gcgggtgcat
    19381 tcgctgccgc ataccattat tcttgatctg acggaagtct ttttgtaaca attcaaacct
    19441 ccttgatgta aacaaatcaa tacaacaaac ggaattgcaa acttacacac gcatcactgc
    19501 gtagatcaaa aaaacaatca ccgcacgagg tttcatgatg gaaggtcaac agcacggcga
    19561 gcagctaaag cgcggcctta aaaaccgcca tattcagctt atcgcgctgg gtggcgcgat
    19621 agggacaggg ctattcctag gtagcgcctc cgtaatacag tccgcagggc cagggattat
    19681 cctgggttac gccattgctg gttttatcgc ctttctgatc atgcgtcagc tgggtgaaat
    19741 ggtggtcgaa gaacctgtcg caggctcctt tagccacttt gcttataaat actggggcag
    19801 ttttgccggt ttcgcctctg gctggaacta ctgggtactg tacgttttag ttgccatggc
    19861 agagctgact gccgtgggta aatacattca gttctggtat ccggaaatcc caacctgggt
    19921 ttctgccgcc gtattctttg tggtgattaa cgccatcaac ctgaccaacg ttaaagtgtt
    19981 tggcgagatg gagttctggt ttgccattat caaagttatt gcggtagtag cgatgatcat
    20041 cttcggcggc tggctgctgt tcagtggcaa cggcggcccg caggcgagcg ttagcaacct
    20101 gtgggatcaa ggtggtttcc tgccgcacgg cttcaccgga ctggtgatga tgatggcgat
    20161 tatcatgttc tcgttcggtg gtctggaact ggtggggatc accgcagcag aagctgataa
    20221 cccggagcaa agtatcccga aagcaactaa ccaggttatc taccgcatcc tgattttcta
    20281 tattggttcg ttagccgttc tgctctcact gatgccgtgg acccgcgtta ccgccgatac
    20341 cagtccgttt gtgctgatct tccacgagtt aggcgatacc tttgtggcga atgcgctgaa
    20401 catcgtggta ctgactgcgg cgctctccgt gtacaacagc tgcgtatatt gcaacagccg
    20461 tatgctgttt ggtctggcac aacaggggaa cgcgccaaaa gcgctggcgt ctgtcgataa
    20521 acgcggcgta ccggttaaca ccattctggt gtctgcgctg gttacagcat tgtgcgtatt
    20581 gattaactat cttgctccgg aatccgcatt tggcctgtta atggcactgg tggtatccgc
    20641 actggtgatc aactgggcga tgatcagtct ggcgcatatg aagttccgtc gcgccaagca
    20701 ggaacaaggc gtggtaactc gcttccctgc tctgctttat ccgctgggta actggatctg
    20761 cctgctgttt atggcggcgg tactggtgat tatgctgatg accccaggaa tggcgatttc
    20821 ggtatacctg atcccggtat ggctgatcgt gttaggtatc ggctatctgt ttaaagagaa
    20881 aaccgccaaa gccgtaaaag cgcattaatc tctctacgcc ctcacccgta cagggtgagg
    20941 gcaataatct ctacaccaac gccccgtaaa tcgtcagtag cgcaatcacc accacgacca
    21001 cgaacgaggt tttcttcgcc attgaaaccg ctgccttcgg cgtctccacc ttatcgacat
    21061 gcggttcacg cgccagagag aactgcgcca gacgcgttaa tacctgatat tgcgaagtat
    21121 ggaaatcacc cagcgaagca aaccaggccg gtaacgcttt ctcaccatga ccgatcaagg
    21181 catataccac acccgcaaga cgaaccggca cccaatccag tacatgaagc actgcatcaa
    21241 tgccggactg taaacgatga tgcggcgtct ggtatcgcgc cagccagtat tgccatgcac
    21301 gcaagaacgc ataccccatc agcgtaacgg gtccccaggt tccccccaca atcagccaga
    21361 acagcggtgc aagataaaaa cgaaagttaa tccacagcaa tgcattttgc agctcacgca
    21421 aatactcacg ttcgtcgcag cctgccggga cgccgtgaat catggtgagt tcgccagcca
    21481 tcgtggcacg ggcatggcta tcattacgtg aagcagctgt cagataagca tgataatgaa
    21541 gacgaacttt acctgcgcca atacacagca aaccaatcag cagccacacc agcagcgtgg
    21601 gaacgttgaa caatactccc tgcaatgcgc gtaacagtaa aaaagtcaca cccatcgcaa
    21661 taatggtcat gcctaacgtg cgcccgagag aaaaatgttt cacccgccga aagaacgctt
    21721 caagacgatg atcaagctgc cagtgctcgc ccaacttaaa caggcgctcg aaaattaaca
    21781 ccagtaaggt tgtaaatagc gtcatgttgt ctccttgctg accagcgcac gaaaccgtgc
    21841 ccaatcaaat gcaggaccgg gatcggtttt ccgatccggc gcaatatcac aatggcccgt
    21901 catgttatta gcgatatcgg gatagcgatc tatcagtgcc cgcgtaaccg ccgcaagctg
    21961 ttgatactgc gcatcggtat acgccagagt atcggtgcct tcaagctcaa tcccaataga
    22021 aaaatcattg cagcgttcac gcccctgata ctgagagact cccgcatgcc aggcacgttt
    22081 atcgaaagga acatactgga ctatttcacc atcacggcga atcaaacagt gagcggagac
    22141 gcgcaaatgg gcgatctcag caaagaaagg atgtgcctgc ggatcaatag ttccagtgaa
    22201 taatgcgtcg atccacggac cgccaaactc gcctggcggc aggctaatat tgtgcaccac
    22261 cagcagggtg ggtgtttcgt catccaggcg gcaatcgtaa tgtggtgagg gaacgcggcg
    22321 cgcgccaacc agccacccct gttctaacaa catgcaggat ctccttatat gtggtgctaa
    22381 tacccggttc agagtagcat gtttctacct tatgattcgt tagctatctg gagttttaac
    22441 atgccgcctc gccgctacaa ccctgacacc cgacgtgacg agctgctgga acgcattaat
    22501 ctcgatatcc ccggcgcggt ggcccaggcg ctgcgggaag atttaggcgg aacagtcgat
    22561 gccaacaatg acattacggc aaaactttta ccggaaaatt ctcgctctca tgccacggtg
    22621 atcacccgcg agaatggcgt attttgtggc aaacgctggg ttgaagaggt gtttattcaa
    22681 ctggcaggcg acgatgtcac cataatctgg catgtggatg acggcgatgt catcaacgcc
    22741 aatcaaccct tgttcgaact tgaaggccca tcccgcgtgc tgttaacggg cgaacgcact
    22801 gcgcttaatt ttgtgcaaac cctttcagga gttgccagta aggtacgcca ctatgtcgaa
    22861 ttactggaag gcaccaacac gcagttgttg gatacgcgca aaaccttacc tggcctgcgt
    22921 tcagctctga aatacgcggt actttgcggc ggcggagcga atcaccgtct ggggctttct
    22981 gatgccttcc tgatcaaaga aaaccatatt attgtctccg gctcagtgcg ccaggcggtc
    23041 gaaaaagcgt cctggctgca tccggatgcg ccagtagaag tcgaagtaga gaatctggaa
    23101 gaacttgatg aggccctgaa agccggagcc gatatcatca tgctggataa cttcgaaaca
    23161 gaacagatgc gcgaagccgt caaacgcacc aacggcaagg cgctactgga agtgtctggc
    23221 aacgtcactg acaaaacact gcgcgaattt gccgaaacgg gcgtggactt tatctccgtc
    23281 ggtgcgctaa ctaaacacgt acaagcactc gacctttcaa tgcgttttcg ctaattgctg
    23341 acagatttat ccgctccatc gcgggcggat aatctcccct tccccaactt tcttcgtaac
    23401 gcctcgcaaa tttcttgtct ttcgctgcaa cgaaataaaa aacctcggaa tctgctttcc
    23461 cgtttcgttt tttgcccctc gcctgtgctg atggcgtttg ccaaagtagc accaaccaaa
    23521 tcaaggagcg aaacagatgg acaagcaacg cggttttaca cttatcgaac tgatggtggt
    23581 tattggcatc attgccattt taagctccat tggtattccc gcttatcaaa actacctgcg
    23641 caaagccgca ctcaccgaca tgctacaaac ctttgtgcct taccgtaccg ccgtagagtt
    23701 gtgcgcgctg gaacatggtg gattagatac ctgcgacggt ggcagcaatg gcattccctc
    23761 gcctaccacc acccgctatg tttcagccat gagtgtggca aagggcgtgg tgtcgctgac
    23821 cgggcaagaa agtctcaatg ggctaagcgt cgtcatgacg ccaggttggg ataacgcaaa
    23881 cggcgtcacc ggctgggcgc gcaactgcaa tattcaaagt gacagcgcat tgcagcaagc
    23941 ctgcgaagat gtcttccgct ttgatgacgc caactaagga gcggcaatga atattccaca
    24001 gctcacggcc ctgtgcctgc gttatcaggg agtcttgctg gatgccagcg aagaagtggt
    24061 tcatgttgcg gtggtcgatg ccccctcaca tgagttgctg gacgcattgc atttcgctac
    24121 caccaaacgt attgagatca cctgctggac gcgccaacaa atggaaggtc acgccagtcg
    24181 cacacaacag acattacccg tagctgttca ggagaagcat cagcccaaag cagagttgct
    24241 gactcgaacg ttacaatctg cgctggaaca acgcgcgtct gatattcata tcgaaccagc
    24301 ggacaatgcc taccgcatcc gcttgcgtat cgacggcgta ttgcatcctt taccggatgt
    24361 ttcaccggat gccggagtcg cattaacagc cagattaaaa gtgctgggaa acctggatat
    24421 tgcggaacat cgcctgccgc aggacgggca attcactgtc gaactggcag gaaacaccgt
    24481 ctcatttcgt attgcgacct taccatgtcg gggtggtgaa aaggtggtat taaggttgtt
    24541 acagcaggtg agccaggcac tggatgttaa cacgctggga atgcagccgt tacaactggc
    24601 ggactttgct catgccttgc aacaaccaca gggactggtg ctggtaactg gccctacagg
    24661 cagtggcaaa acggtcacct ggtgttgtcc acgctacaca ccaattccac cactgaaacg
    24721 ctggtacgtt tacagcaaat gggggtcgcc cgctggatgc tatcatcggc gcttacgctg
    24781 gtaatagccc agcgtctggt acgtaaactt tgcccacatt gtcgccgaca gcaaggggag
    24841 cccatccata ttccagacac tgtatggccg tcgccgctgc cccactggca ggcacccggt
    24901 tgtgtacatt gctaccacgg tttttatggt cgtaccgcct tgtttgaagt cctgcccatt
    24961 acaccagcca tacgccagct tatttccgct aatactgaag ttgaatcgct ggaaacgcac
    25021 gcccgacagg cgggtatgcg tacgcttttt gaaaacggct gcctggccgt ggagcaaggc
    25081 ttaaccacct ttgaagagtt aatccgcgta ctggggatgc cgcatggcaa gtaagcaact
    25141 ctggcgctgg catggcataa ccggcgacgg caatgcgcaa gatgggatgc tatgggcaga
    25201 gagccgtact ttactgctta tggcactaca gcaacagatg gttaccccac taagcctgaa
    25261 gcgaatcgcc atcaattctg cgcagtggcg aggagataaa agcgcggaag tcattcatca
    25321 actggcgacg ctacttaaag ccgggttaac gctttctgaa gggctggctc tgctggcgga
    25381 acagcatccc agtaagcaat ggcaagcgtt gctgcaatcg ctggcgcacg atctcgaaca
    25441 gggcattgct ttttccaatg ccttattacc ctggtcagag gcatttccgc cgctctatca
    25501 ggcgatgatc cgcacgggtg aactgaccgg taagctggat gaatgctgct ttgaactggc
    25561 gcgtcagcaa aaatcccagc gtcagttgac cgacaaagtg aaatcagcgt tgcgttatcc
    25621 catcatcatt ttagcgatgg caatcatggt ggttgtggca atgctgcatt ttgttctgcc
    25681 ggagtttgcc gctatctata agaccttcaa caccccacta ccggcactaa cgcaggggat
    25741 catgacgctg gcagacttta gtggcgaatg gggctggctg ctggtgttgt tcggctttct
    25801 gctggcgata gccaataagt tgctgatgcg ccgcccgacc tggcttatcg cgcggcagaa
    25861 attgctgtta cgcatcccga ttatgggttc actgatgagg ggacaaaaac tcacgcagat
    25921 ctttacgatt ctggcgctga cacaaagtgc aggcattact tttttacagg gcgtagagag
    25981 cgtcagagaa acaatgcgct gcccgtactg ggtgcaactt ctgacacaaa tccagcacga
    26041 tatcagtaac ggtcatccca tctggctggc gctaaaaaat gccggtgagt ttagcccgct
    26101 ctgtttgcaa ttagtgagaa caggagaggc atccggctcg ctggacctca tgttagacaa
    26161 cctcgcccat catcatcggg ataacacaat ggcgctggcg gataacctcg cagccttact
    26221 ggaaccggcg ttgctgatca taacgggagg aattatcggt acgctggtgg tggcaatgta
    26281 tctgccaatt ttccatttag gcgatgcgat gagtgggatg ggataacgct ggcgtgttgc
    26341 tccacgccag cgttgggaga ttacaggttg ttgaagatgc ggttttcttg ttccagcaca
    26401 cgaataaacg tggtgcgctt ggtcagctct ttcaggcgtg aagccccaac gtatgtacaa
    26461 gctgaacgca ggccgcccaa aatatcacgc gcggtatttt caacctggcc tcgcagcggc
    26521 agcttaacgg ttttaccttc tgctgcgcgg tattccgcaa cgccgccaac gtgacgtttc
    26581 atcgcagact cggagctcat gccgtagaac agcataaatt tctcgccgtt ctcctcaacg
    26641 atgcgaccgc cgctctcttc gtggcccgcc agcatgccgc caagcatgac gaaatcggca
    26701 ccgccgccaa aggctttcgc cacatcgccc ggcgtggtac agccaccgtc gctgacgatc
    26761 attccgccca gaccgtgcgc cgcatcggca cattcgatta ccgcagaaag ctgcggataa
    26821 ccgacgcctg ttttgacgcg agttgtacaa acagaacctg ggccaatgcc aactttaacg
    26881 atatcagcac cggagaggat aagctcctca cacatttcac cagtcactac gttgccagca
    26941 caaatggttt tggtcggcca cgcttcacgc gctttcgcaa cgaactgcac gaagtgttcg
    27001 gaataaccat tcgccacatc aatacaaacg aagtttaatg ccgggttcag gtcgagaatc
    27061 tgtttagttt tttcgaaatc cgcatcagac gtgccggtag aaaccatcac atgtttcagc
    27121 acatcagcgg aagaattgtt gataaacgct tgccactctt cgacagaaaa gtgtttatgc
    27181 acagcagtca gaatatcaaa agaagccagc gcagaggcca tagaaaatgt gcctacggtg
    27241 tccatatttg cggcgataat cggcacgccg gaccagctct gacctgaatg tttgaaggtg
    27301 aattgacgtt ccagttcaac atcggaacgg cttttaagag tggagcgttt agggcggatg
    27361 agaacgtctt taaaacctaa cttcagatct tcttcaatac gcatgtgcgg attcctgggg
    27421 ttaatggcta taagggtaag agcgcaactc cagtgacgct atcatacgca ctaatcaatg
    27481 cggcgcaaga ctgcgaaatt gtcttttttt acgctacaat cccataaatt tacctggtaa
    27541 atagtgagcg aacgtttcat caatttcact ttcgcgcgct catttttaac gttttgataa
    27601 tcatgatgaa ttcccgggaa tataaattat ggggtatata gttgccttaa cgggaggcat
    27661 tggcagtggt aagagtaccg ttgccaatgc gtttgctgat ctcggaatta acgtcattga
    27721 tgccgatatt attgcgcgtc aggtggttga accaggtgca cctgcgctac atgccattgc
    27781 tgatcacttt ggcgaaaaca tgattgctgc tgatggaaca ttgcagcgcc gggccttgcg
    27841 cgagcggatc ttcgccaacc cggaagagaa aaactggctt aacgacctgc tgcatccgct
    27901 gattcaacaa gagacgcaac accagatcca gcaagcaact tccccctatg tactgtgggt
    27961 tgtgccattg ctggtagaaa actcactgta taaaaaagcg aatcgagtgc tggtggtgga
    28021 tgtcagccca gaaacgcaac ttaagcgcac catgcagcgc gatgatgtaa ctcgcgaaca
    28081 tgtcgaacaa atccttgctg ctcaggcaac gcgcgaagcc cgccttgccg tggcagatga
    28141 cgtcattgat aataacggcg caccggatgc tatcgcatcg gatgttgccc gcctgcacgc
    28201 acactatttg cagcttgcgt cgcagtttgt ctcacaggaa aaaccgtaat gcagacccag
    28261 gtcctttttg aacatccact aaatgaaaaa atgcgtacat ggctgcgcat tgagtttttg
    28321 attcagcaac tcaccgttaa tttacccatc gttgaccacg ctggcgcgct gcatttcttc
    28381 cgtaatgtca gtgaattact ggatgttttc gagcgcggcg aagtccgcac tgagctgttg
    28441 aaagaactta accggcagca acgtaaactc cagacctgga ttggcgtgcc tggcgtggac
    28501 cagagccgta ttgaagcatt aattcagcag ttaaaagcgg cggtgagcgt attaatttcc
    28561 gcgccgcgta tcgggcaatt tctgcgtgaa gatcgtttga ttgctctggt gcgtcagcga
    28621 ctgagcatcc caggcggctg ttgcagcttt gatttaccta cattgcacat ttggctgcat
    28681 ctaccccagg cgcagcgcga cagccaggta gaaacctgga ttgccagcct gaacccgctc
    28741 acccaggcac ttaccatggt gctggattta attcgccagt cggccccctt ccgtaaacaa
    28801 accagcctga atggttttta tcaggataac ggtggcgatg ccgacttgct gcgcctgaat
    28861 ctgtcgctcg attcacagct ttatccgcaa atttccggac ataagagccg ttttgccatt
    28921 cgttttatgc cgctggacac tgaaaacgga caggtaccgg aacgtctgga tttcgaactg
    28981 gcctgttgct aaggagtaaa aatgtcagaa actattacgg tgaattgccc aacctgcggg
    29041 aaaacggtgg tgtggggtga aatcagcccg tttcggccat tttgctccaa acgttgtcag
    29101 ctgatcgacc tcggagaatg ggctgctgaa gaaaaacgaa tccccagcag tggcgatctt
    29161 tccgaaagcg atgactggag cgaagagcca aagcagtgac atttgtctga tgcctcacgt
    29221 aggcctgata agacgcggac agcgtcgcat caggcatctt gtgcaaatgt cggatgcggc
    29281 gtgaacgcct tatccgacct acagacgctt aagcttcgca attaccggtt cattggctgg
    29341 cggaaaatca tcggcattaa gaccgaccag cgacatccac tcaccgggtt gcccttcttt
    29401 accccacggc cccccttccc aactttcgac cagccagaac cacagagtta tatgcctgtc
    29461 cgggaattca tattccagtt tttcaaatag cgaaaaatgt tgtggggtaa tcccgacctc
    29521 ttcctgaagt tcacgcacca ccgcctgttc cggcgtttcg cccatttcaa ttttaccgcc
    29581 gggaaactcc agtttattcg ccatgtgcgc atctgctgcg cgacgtgtta taaagatttc
    29641 attgttctcg ttgcgaataa tacctaccgc aatttgcagc tttttcattg tcttaaacct
    29701 ataaaaaagg cgcagaatcc tgcgcctttt acttcaactg ttagctttta ttgcaggcga
    29761 ccatggcact gcttgtattt tttaccagaa ccgcacgggc aaggatcgtt acgtcctact
    29821 ttgcgttcac cggtttgcgc cgccagtgca gctgcggctg cagagtcgtc atcctgatgg
    29881 ctaagctgct gcatttgtgc taaacgctcg gcttccatac gacgctgttg ttccagctcc
    29941 tcaacctctt caggcatacg tacctgaact ttgctcagcg tactgataac ttcatatttc
    30001 aacgactcca gcatcgctgc aaacatggag aacgattcac gtttgtattc ctgcttcgga
    30061 tctttctgtg catagccacg caggtggata ccctgacgca gatagtccat cgctgccagg
    30121 tgctctttcc acagggagtc aagcgtttgc agcatgacgc ctttctcgaa gtgacgcatc
    30181 atctcagcac caaccacttc ttctttacgc tgatacactt cgatggactg cgccagaatg
    30241 cgctcacgca gcgtctcttc atgcagttct ggttctttat ccagccactc ggtaattggt
    30301 aaatcgaggt cgaaatcgtt cttcagacgt tcctgcagcc ccggaatatc ccacatttct
    30361 tccagcgact gtggcggaat gtaggcatca atggtggctt tgaacacatc ttcacgaatg
    30421 ctgttgatgg tttcgctcac atcgctgaca tccagcagtt cgttacgctg ggagtaaatg
    30481 gcgcgacgct gatcgttagc cacgtcatca tattccagca gttgcttacg aatgtcgaag
    30541 ttacgacttt caactttacg ctgggcgttg gcaatcgctt tagtcaccca cgggtgttca
    30601 atggcttcgc ctggcttcat acccagttta cgcatcatgc cggatactcg gtcggaagca
    30661 aaaatacgca tcagcgcatc ttccatcgac aggtagaaac gggaagaacc agcatccccc
    30721 tgacgaccag aacgaccgcg caactggtta tcgatacgac gggattcgtg acgctcggta
    30781 ccgatgatat gcaggccacc tgcttccagt accgcatcgt gacgtacctg ccagtcggct
    30841 ttaatttttt caatttgctc tgcggtcgga ttttccagcg cggcaacttc tgcctgccag
    30901 ctacctccga gcacaatatc tgtaccacga cccgccatgt tggtcgcgat agtcaccgca
    30961 gccggataac ctgcctgagc aacaatcgcc gcttcgttgg cgtggaattt ggcgttcagg
    31021 acattgtgct taataccggc tttggtcagt tcgtttgaca ccagctccga tttttcgatg
    31081 gagatagtac ccaccagcac cggctggcct ttcgcagtac gttctttgat atcttcaatg
    31141 atcgcctgaa ttttttccgc ttcagtcatg tagaccaggt ccggcagatc tttacgaatc
    31201 attggacggt tggtcggaac aacgacggta tccagtttgt agatggagct aaattcgaaa
    31261 gcttcggtat cagcagtacc ggtcatcccc gccagttttt catacagacg gaagtagttc
    31321 tggaaggtga tcgaagccag cgtttggttt tcgttctgga tctgcacacc ttctttcgct
    31381 tccacagcct ggtgcagacc atcggaccag cgtcggccct gcatggtacg accggtgtgt
    31441 tcgtcaacga tgataacttc accatcttta acgatgtagt cgacgtcacg ggtaaacagc
    31501 gcatgagcgc gcagcgccgc cgttacgtgg tgcatcagca tgatgttggc cggagagtac
    31561 agagactccc cttcatccat gatgccctct ttcaccagca gttcttcaat cagcaccaga
    31621 ccacgttcgg tcaggttcac ctggcgagat ttttcgtcca ccgagaagtg gccttcgccc
    31681 tggaaggttt cggagtcttc tttttcctga cggatcaggt gcggaataat tttattcacg
    31741 cgtttataca tttccgagct gtcttctgcc gggccggaaa tgatcagcgg tgtacgcgct
    31801 tcatcgatca ggatggagtc cacttcgtcc accagcgcat agtgcagttt acgctgtaca
    31861 cgttcttcag ggctgaacgc catgttgtcg cgcaggtagt caaagccgta ttcgttgttc
    31921 gtaccgtaag tgatgtcagc tgcgtaagct tcgcgctttg ccggtgctgg catgcccggc
    31981 aggttgatac caacagtcag gccaaggaat tcaaacagcg gacggttgtt ttcggcgtca
    32041 cgttgcgcca ggtagtcgtt gacggtaact acgtgcacgc ctttaccggt tagtgcgttc
    32101 aggtaagcag gcagcgttgc ggtcagggtt ttaccttcac cggtacgcat ttcggcgatg
    32161 cagcgttcgt taagaaccat accgccgagt aactgaacgt cgaagtgacg cataccaaag
    32221 acgcgcttac ttgcctcacg caccacggcg aaagcttccg ggatcagatt ttccagcact
    32281 tcgccttttt ccagacgcgc acgaaactct gcggttttcc ctttcagttc ttcatcggag
    32341 agtttttcca tctccggttc catggcattg atgatgttga ccactttgcg catccggcgc
    32401 agggtgcgat cgttacgact accgaaaact ttagttaaca atttgattag cataataaaa
    32461 tctcaaacgc cccgcgttac ggagttaata aaatgaagta aaggtttatt gttgttaggt
    32521 gaggcgttga gggccagcac ggatgccttg cgcctggctt atccagacgg gcgtgctgaa
    32581 ttttgcttgt ggggtaaaat gcgcataatc aatgcgataa cccttttcag acggcgtgcc
    32641 ttcctgggtc agcagcgcgc tgagcgtatc cagtaatgca agatgttgcg cctgaagagg
    32701 caaagattct tcagcaacgg gcagtgtttg cggtgccatt gcgaaagaaa gatgacggat
    32761 taccgtacga atagcatgtt gatgccagta atcaacggaa tagttcggat tcgggcggcg
    32821 cgtgttcgct tccagcaagg ccaattgacc aaagttaact ttggctgaag gctcgtggtt
    32881 gcgggttgtc gcttttgcgg gcgcgtttgg ttcggcggcg ttgctgagcg caggcaaacc
    32941 taaactcgcc gcgaccatcc ctaataagag atgcggccag aagtagcgtt taccaaactg
    33001 tcgccagcgc gtcagtattc cactcacgtt attgccatcc cgatcgaatt caaacgtatt
    33061 tagcgcacaa atcttatcat taaagcctga agtccacagc acgaatgaag ataagttgtg
    33121 cgtaaaagtg cttaagaaaa cagcgttcgc accgtttttt atagctctta taaaattcaa
    33181 aaaaaaacga ctggttcacc tggccggaga gagtgccaga tttgccagtc gaattttata
    33241 cgacagtata aatgtcgtta tgccagtaca gctgaaggcg ctttgaaggc caacggcagt
    33301 tctgcgtcgt cctggaaggt cacatattcc caggcttcct gtttcgccag gacagcctgc
    33361 agcagtttgt tattcagtgc atgaccggat ttataagcgg taaatgcacc aataatattg
    33421 tgaccacaca tgaacaagtc accgatcgca tcgagcattt tgtgacgcac aaattcgtct
    33481 tcaaaacgca ggccgtcttc gttcagtacg cgataatcgt caacaacgat ggcacaatcg
    33541 aagctgccgc ccaggcacaa accacgggac tgcagatatt cgatatcacg catgaaaccg
    33601 aacgtacgcg cacggctgat ctggcgcata aacgcatcag cggagaagtt catcgcatag
    33661 cgctggttgc tggaatcaat agccggatgg ttaaaatcga tggtgaaatc cagcgaaaaa
    33721 ccattgtacg gcttaaattc agcccactta tcgccatctt cgacacgaac agtctctttg
    33781 atgcgaacaa attttttggc gcagttcaac tcgtcgatac cggcgtcaag cagcaggtat
    33841 acaaacggag cggcgctgcc gtccatgatc gggatttccg gcgcgttaac ttcgataaca
    33901 atgttatcga tgcccaggcc cgcgagagca gcattgaggt gctctacggt tgaaatccgt
    33961 acatcatgct cgttgaccag acacgtacag agcatggtat cacgcacaga tttggcatcg
    34021 gccgggaaat ctaccggtgg attcaagtcg gtgcgacgat agatgacccc ggtgttggcc
    34081 ggcgcagggc gtaacgtcag ggtgactttc ttgccggtat gtaaaccgac acccgtcgcc
    34141 tgaacgatac gtttaagtgt cctttgtttg atcatcgtat tatctcgcca aattacctat
    34201 ccaaccgaag tgtactatac attcggcggg ccagtttagc acaaagagcc tcgaaaccca
    34261 aattccagtc aattcttaat cagcttgctt acgtaggaat gctgggatat ccagataatc
    34321 cggctctttc gcagtttgcg gcgcattgtc attcactact ttagcaaccg gcttctgctc
    34381 ctgggtcagc ggagccatcc catgctgctg gtagcgatcc atcactggct gctgaacctg
    34441 cttattggtc accagagtga tttcaggacg tttgtccatg ccgatacctg tcgcaacaac
    34501 ggttacgcgc agctcgtcat tcatatccgg gtcaagagaa gtaccgataa ccacagtcgc
    34561 gttgtcggaa gcaaatgcac ggatggtgtt acctaccgtt tcgaactcat ccagacgcag
    34621 gtcgaagccc gccgtgatgt taaccagcac gccgcgcgcg ccagacaggt cgatatcttc
    34681 cagcagcgga gaagagatag ccatttcagc agcttcttcc gcacggtctt caccgctcgc
    34741 cacgccagaa cccatcattg cgtagcccat ttcagacatt acggtgcgta cgtctgcaaa
    34801 gtccacgttc atcaaacccg gacgagtaat cagttcggcg ataccttgca cagcgccttt
    34861 cagtacatcg ttcgctgcgc caaacgcatc cagcagggag ataccgcggc ccagaacttt
    34921 cagcagtttg tcgttcggga tagtgatcag agagtccaca tgcttggaca gttcagtgat
    34981 cccctgctcg gcgaatgcca tacgcttctt gccttcaaag ttgaaaggct tagtgacgac
    35041 agcaacggtc aggataccca aatcttttgc cacttcagcg acgactggtg ctgcacctgt
    35101 accggtacca ccacccatac ccgcagcaat aaagaccatg tctgcacctt ccagcgccgc
    35161 acgcaatgca tcgcgatcct catcagccgc attgcggcca acttctggat tagcgccagc
    35221 gcccagtcct ttggtgatac cgctaccgat ttgaatcgtc tgtccaaccg ctgttttacg
    35281 cagcgcttgt gcatcggtat ttaccgcgaa gaattcaaca ccttcaatac gctcgcgcac
    35341 catgtgttca acagcattac cgccgccgcc gccgacgccg atgactttaa tcaccgcgtc
    35401 attggtaagt tccattggtt caaacatagt ttctctccga tttgtgcctg tcgcctgagt
    35461 ccgtaatcat cgtcggcctc ataaaaatta aaactctttt cgcagccaac tattgagtcg
    35521 cttgatccac gagccaactg atgctgtaac acgtttttct acttcagctt caccgttaag
    35581 atgtgactct ttcccatagt gaagcaatcc caccgccgtc gaataatacg gctcctgagc
    35641 ataatccgtt aaaccggtaa tgttcagcgg cgcgccgata cgcacttgcg tatgaaacac
    35701 gcgctgagca caggctgcaa gaccttcgat ctgcgctgcg ccaccggtta atacaatgcc
    35761 tgccgccagg tgatgtttaa ccccttgttg gcgaagcttt tcctgcaact gcaatatctc
    35821 ttcgttgacc aggttgagca actcggtata gcgcggctcg atcacctctg ccagggtctg
    35881 acgttgcaga ctccgcggcg gacgaccgcc tacgctcggc acttccacgc tctcatcttt
    35941 tccaacgatg gaacccagcg cacaaccgtg gcgaacttta atcgcttcgg cgtcgcttgg
    36001 cggcgtgcca aaggcgtaag cgatatcact ggtcacgaca ttgccagcat aaggaattac
    36061 ctttgtgtgg cgcaatgccc caccggtata aacggcgata tccattgtac caccaccgat
    36121 atcgacgacg cagacaccca gttcacgttc atcttccgtc aataccgaat aacttgatgc
    36181 cagtccggca aatatcagtt ggtcaacttt cagcccacaa cgttcaaccg ctttgacgat
    36241 gtttttcgcc atatcgttgt gacatgtgat caggtgcact tttgcctgca tccgcacacc
    36301 cgaaagtcct accggattct tgatcccttc ctgatagtca atcgcatact cttgcgggat
    36361 cacatgcagc acacgatgct catcgcgcac acgcaccgat ttcgcggtat ggacgacgtt
    36421 ttccacatct tcttgcgtca cttcttcttc agaaataggc accataccaa tttcattctg
    36481 gcagctgatg tgcttaccag aaagcgccag atataccgaa gagatctgac aatctgccat
    36541 caattctgcc tggtcaatgg cgcgttgtac gcacttgacc acggattcga ggtcgttcac
    36601 cccgccttta tccataccac gtgacgggca gctgcccacg ccaatgatat tgaccatacc
    36661 gtcgggcaga acttccccta ctaaagcggc aaccttcgcg gtaccaattt ccagtcctac
    36721 taccagtttt ctgtccgtcg ccttgatcat tgttgttctg cctgtgcctg attttgttgc
    36781 tgagtagatt cctctggcgg caagggcgcc cagcctactg ccgctccaga gtcataacgc
    36841 aaatcaacgt agctaatccg tttgccatcg gtttgcgcct gctgctgtaa aaccggataa
    36901 agttctacaa agcgaaccaa acgtttcatc gtatcgcccc ggccaagatt gagcttaata
    36961 tcgttattca gcgtcaactg ccaggaacgc cgcgcggtca tcgccgcttc cttcagagta
    37021 aatctgtcct ttgccagcat ctgccccatt tcgcgatagc cctgcaacac ttcattagcg
    37081 ctgccttccg ggccatacag cattggaagc acctgcttgc tggtgcgatc tggcggcacg
    37141 ctgaaggtgt ttccttccgc gtctaccatg tgttgatcat tccaccgcgc aatcggcaca
    37201 tattcaacca gatgaatctt caattcatca ggccactgct ttctgacgct cacctgctta
    37261 atccacggca ggcgttgttc tatttgcgtc tggatgatgt tgacatcctg ggtcataaag
    37321 gtacccggct cacccaatgc caggatcgac tgccggatat cgtcattacg cgtgtaatgg
    37381 cgttcaccgg tcaacaccag ctttgagagc ggcaggcgtt gcgcatcttc catccagctc
    37441 aacacgaccc agccgctcac caacactgtc gttaaaacgg tcagcaggaa aaggatcccc
    37501 gccagacgcg ttccattatt gcggcgagaa gaaacctctt cttcgctgtt tcgcgtgttc
    37561 agagcagcct gcgacatatt agtccgccag ttccagaatt cgtactacca actgcgagaa
    37621 gctcatacct gcctgacgtg ccgccatcgg caccaggctg tggctggtca tacccggtga
    37681 ggtattggct tccagcagat aaaactgtcc atcactgtcc agcataacgt caatacgtcc
    37741 ccatcctttg caacctaacg tcgtccatgc tttcagcact aatgcctgca aattggcctc
    37801 ttgtgacgct tccagacctg cggggcagaa atactgtgtc tcatcagaga gatacttcgc
    37861 ctcataatca tagaaggttc cggacggttg aatacgtatt gacggtaaaa tttcttcacc
    37921 gagtatcgca accgtgaact ccggcccact tagccatttt tcaatcaata cttcttcatc
    37981 gtgctgaaat gccaatctta atgcatcttg tagagcattt tctgctacta cttttgacat
    38041 tcccacactg gaaccttcgc ggctcggctt aacgataacc ggcaaaccca gagcagaaat
    38101 ttctgctaac tgcttatcgc tcaggccttt ttcaaactct gcgcgggtta acgctaccca
    38161 cggcgcgacc ggtaaaccgg caccttgcca cagaagtttg ctgcgtagtt tatctattga
    38221 aagcgcagat gccatcactc cgctgccggt ataaggcaag cccatcagct cgagcatgcc
    38281 ctgcagcgta ccatcttcac cgccgcgacc gtgtagcgcg ataaacactt tctgaaaacc
    38341 catcgacttc agttgcgtca cgtcgacttc tttcgggtcg acaggatacg catcaatacc
    38401 gccttcacgc agcccggcta acaccgctgc gccagaattc agagaaactt cccgctcagc
    38461 ggaagtcccg cccaacagga ccgcgatttt atcagtcatg ttgttcttcc tccggagttt
    38521 gcggcttcag tttgatttca gctaaagaac gggcaatttt tccgatatta ccagccccct
    38581 gaacgagaat caggtcgtta ccggttaata ccggtgccag catctcggct acctgcgcag
    38641 ggtccggcac cagaatggga tcaattttcc cacgtccacg aattgtgcga cacagcgaac
    38701 ggctgtccgc tcccggaatt ggcgcttcgc cagccggata cacttccagc atcaacaggg
    38761 tatcaacctg cgtcagcaca ttggcgaaat catcatacag gtcgcgcgta cgggtaaaac
    38821 ggtgcggctg aaacagcatt accaggtttt tatccggcca gcctgcgcgc gccgctttaa
    38881 tggtggcgtc aacttccgtc gggtggtggc cgtagtcatc gaccagcatt gccgtaccgc
    38941 ttttaccatt cactggctcc agcgggaatt caccgaggaa atcaaaacgg cgaccagtcc
    39001 cctggaagct ctccagcgca cgcaaaatag cctcgtcgtc aattccctct tccgtagcaa
    39061 ccgcaaccgc agctgcggcg ttcagcgcgt tatgacggcc tggcgcattc agggtgacgc
    39121 acatcggctc tttgtcctga cgcagcagcg taaagtgccc ctgcgggcca atctgctgat
    39181 aatcttctac acgcacgtcg gcatcttcgc tgaagccgta agttgtggtc tgacgtccca
    39241 cacgcggtaa caattcgcgg atcaccggat catcaacaca catcaccgca cgaccgtaaa
    39301 aaggcaggtt gtgcagaaaa ttaataaagg tctgttttaa attctcaaag tcgccctggt
    39361 aggtatccat gtggtcggct tcgatattgg tgacaatcgc caccatcggt tgcagatgca
    39421 ggaacgatgc atcactctca tctgcttcgg caatcaggta ccgaccatgc cccaaacgcg
    39481 catgaacccc cgccgctttt accagcccgc cgttaacgaa ggttgggtcg agccccgctt
    39541 ctgcgtagat gctggaaacc atcgcggtag ttgtcgtttt gccgtgcgtt cctgcaatgg
    39601 cgatgccatg acgaaaacgc attaactcag ccagcatttc ggcacgacgg atcaccggaa
    39661 tacgcgcttc atgagctgcg acaatttccg ggttgtcggc agaaatggcg ctggaaacaa
    39721 cgaccacgct ggcatcacgt acgttttccg ggcgatggtt gaaataaatc gtcgcaccca
    39781 gattcagtaa ctgctgcgtg accggatttg gcgctaaatc ggaaccactg atctgataac
    39841 cttcattggc cagaacttcg gcaataccgc ccataccggc accaccaatg ccgacaaaat
    39901 gtatgtgccg aacgcgacgc atttcgggca cgatggaacg cagttttgcc aattgttgtg
    39961 tattcattct ttacgccatt aacttcttaa attcatacga tgcaaaaggc atcgctacaa
    40021 ttacgcccgg gcagcccggc tcacttcatt tgccactcgc tcggtggcat ccggaatgga
    40081 tgcagcgcgg gcgcgttctg ccatggttaa taaggtttct cgcgaccacc cggccagggt
    40141 gttggcgaca gcatccacgc taagctgtgg ctgctcgata attttggctg cgcccgcttt
    40201 ttccagcggt agcgcattcc agtattgctg gcggtcttta tgttgaaacg gcacaaacaa
    40261 cgccggtagt cctgccgcgg cgatttcact cacagttaaa gcaccggagc ggcaaacgac
    40321 gacatccgcc cacgcatacg ccgccgccat atcatcaata aattcagtca ctttatgctg
    40381 cggttgcccc gcttcggcat acgcctgttc aacggattgt tgcgaacctt tgccgctctg
    40441 atgccagata gtgactgaat cacccagttt cgcagcaacc tgcggcattg tctggttaag
    40501 aatgcgtgcg ccctgggaac cacccactac caacacacga accggacctt cacgtccagc
    40561 caaacgttgc tgcggcaacg gcagcgccag cacatcggta cgcaccgggt tacccaccac
    40621 ttccgcatta gggaaagcgc ctggaaacgc ctgcatcact ttggtagcaa tcttcgccag
    40681 ccatttattg gttaagcccg caataccgtt ttgttcatga agtacaaccg gaatgcctaa
    40741 cgaccacgcg gccagaccac ctggacctga tacatagccg cccataccga gtaccacgtc
    40801 aggtttgtac gctttcataa tcgcccgcgc ctgacgccag gcgttgaaga tacgcagcgg
    40861 cgcagctatc agtgctttta taccttttcc acgcaaacca gagatacgaa tgaaatcaat
    40921 ttcgatgcca tgttttggca ctaagtccgc ttccatacgg tcggcagtcc ccagccagcg
    40981 aacttgccaa ccctgagcca ttagatagtg cgcaaccgcc agtcccggga atacatgtcc
    41041 accggttccg cctgccatca ccattaatcg ctttccttga ccactcatcg tgaacctcgt
    41101 acaaacgcct gcgctttctc cagacgcgtt tcataatcaa tacgcaacag catcatgatg
    41161 gctgtcgaca taatcagtaa gctcgaacca ccgtaactga ttagcggcaa tgtcagacct
    41221 ttggtcggta acatccccgc cgccgcgcct acgttaacca gcgcctggaa gctaaaccag
    41281 atgccaatag aacaggcgag aaaaccggaa aaacggtggt caatttctaa tgctttgcgg
    41341 ccaatcgaca tcgcacgaaa agcgacgaag aataccatta aaagtgctag taccacaccg
    41401 acataaccta attcttcgcc gataatggcg aaaataaagt cagtgtgcgc ttccggcaga
    41461 tactccagtt tttgtaccga attacctaaa ccttgccccc aaagttcgcc gcgaccaaac
    41521 gccatcagcg attgcgttaa ctgatagccg ctgccaaagg gatcttccca cgggttccag
    41581 aatgcggtaa cacggcggat acggtacggt tcggcgagga tcagcaacac aaccgctgaa
    41641 atgcccatac cgataatggc aatgaactgc cacaatttcg ctcccgccag gaacaacatc
    41701 gccagcgtag tcacaaacaa caccaccacc gtaccaaggt ctggctgtgc cagcagtaac
    41761 actgccaaca ccagaatcac gcccatcggt ttcaggaagc cgcgcaggtt attacgtact
    41821 tcgtcgcctt tacgcaccag atagttggcg atatagcaaa acagcgacag ttttgtcagc
    41881 tccgcaggct ggatacgcag caaaccgaga tcgatccaac gcgatgcccc tttaacagcg
    41941 ctacccacta ccagcacgat catcagcagg atgatagatc cgagcagcat cgtggcactg
    42001 tagcgttgcc agaactccat cggcagacgc agcgtaatga tcgccagaat aaacgccaaa
    42061 atcagataga caccatcacg cttcgcgaag aagaacggat cgttggttaa gcgttgccct
    42121 atgggcattg acgccgaggt caccatgata aagccaatcg ccgcgaggcc gaaggtcagc
    42181 cacagtaagg tgcgatcgta catgatcagg ctgtcggtat ctttttcccg cgagcccatc
    42241 acccagccct ttagcgccgt ggagatccag accaggatac tgaatcctgg caggcgcggc
    42301 attttcaggc gagggagaga taaacgcatc aacctaactc cttcgccaga cgggcaaact
    42361 cattgcctcg ttgttcaaag ttcttgaact gatcaaggct ggcacaggcc ggggagagca
    42421 gaaccatatc gcccggctga acacgcggag ccagcaagcg catagcctgt tccatagttt
    42481 cggtttgttc tgccacttcc gggcgtagcg ccgccagctg cgcgccgtca cgaccgaaac
    42541 aatacagacg tacgttatcg ccattcaggt aacgcaccag tgggctaaag tccgccgatt
    42601 taccatcgcc gcccagcaac aaatgcagcg tgccgtctac gtgcaggcca ttcagcgccg
    42661 cttccgtact gccgacgttg gtcgcttttg aatcgttaat ccagcgcaca ccgttatgct
    42721 ccagcaccac ttcaaagcga tgcggcagac cggtgaaggt ggttaacgct ttcaggctgc
    42781 tggcgcgcgg taaccctgcg gcatctgcca gcgccagcgc caccagcgca ttggtgtagt
    42841 tatgctgccc ggaaagtttc atctctttca cattcagcac tttctcgcct ttaacccgca
    42901 gccaggtttc gccctgctga tgattcaggt gatagtcacc catgttgacg ccaaagctga
    42961 cgcagcgttc atccgcaccg cgaatcggca ttgttaaggc atcatcagca ttaaccacgc
    43021 aaactttcgc gttttcgtaa atgcgcagtt ttgctgcacg atactgttgt aaaccaaacg
    43081 ggtagcgatc catatgatct tcagtcacgt tcagaatggt cgctgctacc gcctgtaagc
    43141 tggaggtggt ttccagctgg aagctcgaca gttccagcac gtacagttca cactcatcat
    43201 ccagtagcat caacgcaggc aggccaatat tgccacccac accaacgtta acccccgccg
    43261 ctttcgccat ttcacccact agcgtggtga ccgtgctttt gccgttagaa ccggtaatcg
    43321 ccacaatcgg tgcttgtgct tcgcgacaaa acagctcgat atcgccaacg atttcgattc
    43381 cggcatcagc ggcagcgctt aaggatggat gcgccagtgc aataccggga ctggcgacaa
    43441 tcagatctgc cgccatcagc cattcatcat tcagactgcc cgtgtggcgt tctacggctt
    43501 cgggtaattt atccaggcca ggcggtgtca tacgcgtatc cataacgcgc ggcgtcacac
    43561 cgcgagcgag gaaaaagtcc acgcaggaaa gcccggtgag gcccaggccg ataatgacaa
    43621 catttttacc ctggtaatca gccatgatta acgtaccttc agcgttgcca gaccaatcag
    43681 gaccagcatc agcgaaataa tccagaaacg cacaatgacg cgcggttccg gccagccttt
    43741 cagttcatag tggtgatgaa tcggtgccat gcggaaaata cgttgtccgc gcagtttaaa
    43801 ggagccgacc tgcaggatga cagaaagcgt ttctaccacg aacacgcccc ccataatcac
    43861 cagcaggaat tcctgacgta gcagtacggc gataatgcct aacgcaccac ctaacgccag
    43921 cgaaccgaca tcgcccataa agacctgcgc cggataggtg ttaaaccaca ggaagcccag
    43981 tcctgccccg actatcgcgg tacagacaat aaccagttcc ccggcgtgtc gcagatacgg
    44041 tatgtgtaaa tagctggcaa agttcatgtt gccggtcgcc cacgccacca gcgcaaaacc
    44101 accggcgaca aatacggtcg gcataattgc cagaccgtca agaccatcgg tcaggtttac
    44161 cgcgttgcca gtacccacaa tgacgaagta agccagcaga atgtagaaca gccccagttg
    44221 cggcatcaca tctttaaaga atgggaccac cagctgcgtt gcgggcgtgt ctttgccggc
    44281 aaggtacaaa gcgaaagcaa cacccagcgc aatgaccgac atccagaaat atttccaacg
    44341 agcgatcaac cctttggtgt ctttacgcac cactttgcga taatcatcaa caaagccaat
    44401 aacaccgtaa cctaccagca ccaccaacac gcaccagacg tacggattgg acgggtaagc
    44461 ccacagcagc acggagatca caatcgctgt caggatcata atcccgccca tggtcggcgt
    44521 accgcgcttg ctgaagtgtg attcaggacc gtcgttacgc actacctgac caaaggaaag
    44581 tttttgcaaa tgagcaatca tgcgcgggcc catccacaat gagatgaaca gcgcggtcag
    44641 caggctgacg atggcgcgaa acgtcagata ggaaaagacg ttaaagccgg aataatattt
    44701 gaccaaatgt tcggccagcc aaactaacat gtcccattct cctgtaaagc gcgtactacc
    44761 tcttccatgg cagcactacg tgaaccctta actaaaatcg taattacctg ttgctcagca
    44821 atcagtgatt taagacgcgt aattaacgca gttttatcag caaaatgttc gccaacgccg
    44881 ctggcggtgc tgatagcatg gctttgttta cccatgctta acacgcggtc aataccagca
    44941 gcttttgccg cctcacccac ctgtacatgg caggcttcgc tttcagcgcc cagttccgcc
    45001 atatcgccca ccaccagcac gcggtagccc ggcatttcag ccagtacctg aacagctgcg
    45061 gtcattgaac cgacattggc gttgtaggag tcgtcgagca gcaactggtt ttctgccagt
    45121 tggatgggga acagacggcc aggaacagct ttcagatttg ccagccccgc tttgatagca
    45181 tcaagcgttg cgcccacgga catggagagc gcagcggctg ccagcgcatt cgcaatattg
    45241 tgacgccccg gcaacggcag cagaacatcg acgctaccgg ttggggtttg tagggtaaat
    45301 tccataccgt gcgaggtcac atggatattg gtggcggtga aatcgctgtt ggcggcattg
    45361 ggtgagaaac gccacacttt gcgtgagcca attacgctct gccagttcag ccagtcgttg
    45421 ttgtcggcgt tcataatggc gataccgttt tccggcaggc cgctaaagat ttcacctttc
    45481 gctttcgcga cacccgcaag cgagccaaaa ccttccagat gcgccgctgc caggttgttg
    45541 accagcgccg cttccgggcg agtcagactc acagtccagg caatctcgcc ctgatggttc
    45601 gcgccaagtt caataactgc gtaatcgtat tccggcgtta agcgcaacag cgtcatcggt
    45661 acaccgatgt cgttgttgag attgcctgcc gtataaagcg tgttgccgca ctggcttaaa
    45721 atcgccgccg tcatctcttt aacggaggtt ttgccggagg accccgtcag agcaaccacg
    45781 agcgccggaa cttgctggcg aacccatgca gccagttcac caaacgccag acgcgtatcc
    45841 ttgacgatta actgcggcag gtcgatgtcc agcggacggc taaccagtag tgcgcctgcg
    45901 ccgccagctt tcgcctggtc ggcaaaatcg tgggcatcaa aacgttcacc tttcagggca
    45961 acaaacaggc agcccggcgt cagttttcgg gtatcagtgg ttacagcatc aagggtgata
    46021 tctgcacctt gcagttcacc gttgagaata tcggtaagtt ggctaagggt tacgctaatc
    46081 atgcaatccc ccccagcaga cgcgccaccg tgacgcgatc ggagtagtcc agacgctgat
    46141 tgccaacaat ctggtaatcc tcatggcctt tgcccgcgac cagtaccaca tcattctctt
    46201 tagcctgcat aacggcgcaa gtcaccgctt cagcacggcc ttccatcact ttggcatgtc
    46261 cggcatctaa cattcccgcc agaatatcgt tgatgatggc acgcggttct tcggtacgcg
    46321 ggttatcgtc cgtcaccacc gccacgtcag caaactcttc ggcaattgcg cccatcagtg
    46381 gacgcttacc tttatcgcga tcgccaccac agccaaagac gcaccacagc ttgcccgcac
    46441 agtgcaggcg cgccgcctgt aaggcttttt ccagtgcatc cggcgtatgc gcgtaatcca
    46501 ccaccaccgt cggtttgcct ggcgcagtga acacttccat acgtccgcaa accggttgca
    46561 gacgcgcggc ggttttcagc agatcagcca gtggatagcc cagcgccagc agtgttgcca
    46621 gcgcgagcaa caggttgctg acgttaaaag cgcccatcag atggctttca atttcgccat
    46681 cgccccagct tgaactaaag cgaatggtcg cgccgctgtc gtgatagttc acttcggtcg
    46741 ctttcaacca gcgtccgtga cagttcggat tgatatgatc ttccattgat accgcaaccg
    46801 cgtccggcag ttttgccagc cagcggcggc ccacttcatc gtcggcgtta ataatcgcct
    46861 gaccgcaatg atgttcagaa taaagcagcc atttcgcagc ttcgtagtgt tccatatcac
    46921 catgataatc aaggtgatcg cggcttaagt tggtaaagac cgacgccgga aatttcaatg
    46981 ccgccacacg gtgctgtacc agcccgtggg aggaaacttc cattgcgcaa aaagtcgcgc
    47041 cctgatccac cagccccgcc agctcatgct gaacatcaac tgccgaaccg gtagtatttt
    47101 ctgtcgggat cactttcccc agcagaccgt taccaacggt gcccattacc gcgctggttt
    47161 cgccaagcag ttggctccac tgcgccagaa gctgggtcgt cgtggttttg ccgttggtgc
    47221 ccgttacgcc tacgagacgt aaattgtcag agggttcatg gtaaaagcgg cccgccagtg
    47281 cagataaacg ctcgttgagc tggctgagat agatgaccgg tacgccgtgc atttcacgga
    47341 tttcaccgtc ggtcgcctca tctttcgcct ctgcaataat ggcagccaca ccttgcgcta
    47401 tcgcctgcgg gatatatcga cgcccgtccg cctgatgacc tactacagct acaaagagat
    47461 cgcccgccgc agccacacgg ctgtcgagtg tcatctctcg cagtgctcgc gaaggtgcgt
    47521 ctggcaccca cggagcaaga aggtcgcgca aattacgatc tgccacctgt cccctcgcct
    47581 tgattaatca caaattcatt tttatcgccc gttgtcagcg catccggctc gatgttcatg
    47641 gtacgcaata cgccgcccat gatggcacca aagaccggcg cggaaacggc gccgccgtag
    47701 tatttacccg cctgcggatc gttgataaca acaaccagcg cgaagcgcgg ctgactcgca
    47761 ggcgcaacgc ctgcggtata agcaatatat ttattgatgt agcgaccgtc cggcccgacc
    47821 tttttcgcgg taccggtttt aatggcgata cgatagcctt taatcgccgc cttcacgccg
    47881 ccgccgcctg gtagcgccac gctttccatc atatgcacca cggtgcgaac aatggattcc
    47941 gggaagacac gttcaccggg aaccgggggg tcaactttgg taatcgacag tgggcgataa
    48001 atgccgtagc tgccgatagt tgcgtagact cgcgctaact gtaacggtgt taccattagc
    48061 ccgtagccga aagagaaggt ggccctctct atgtcagacc accgttgttt ttgaggatat
    48121 aagccactgc gttctccgac caaccccaaa ttggtcgctt ttcccagtcc aaaacgtgag
    48181 taagtatcta ctaacgctga ggacggcatc gttaacgcca gcttggaaac accgacgtta
    48241 ctcgacttct gtaatacccc ggtcagggtt aattcgctgt agcgtgccac gtctttgatt
    48301 tcgtggccgt taattcgata aggaatggta ttgagtaccg agttttcccg caccacgcca
    48361 cgttgcaacg cggtcatcac caccatcggt ttaaccgttg agcccggttc aaacacgtcg
    48421 gtgatggtac ggttacgcat cgcctctttc ggcgtgccgc tcagattgtt agggttgtat
    48481 gacgggctgt tagccatcgc cagcacttca cctgtgttga catccaccag cacggcgcta
    48541 ccagattcag ccttgttaaa ggccaccgcg ttgttcagtt cgcgataaac cagcgcctgc
    48601 aggcgttcat caatactcag cgccaggttg tgcgctgcct ggctgtcagt agaagaaata
    48661 tcttcaatta cgcgaccata gcggtcttta cgcacaatgc gctcacccgg ctgcccggta
    48721 agccatttat cgaaactctt ctcaacgccc tcaatccctt gactatcgac gttagtaaag
    48781 ccgatgaggt gagcagtcac ttcgccggac ggatagtaac ggcgagactc ttcacgcaga
    48841 tgaatccccg gcagtttcag ttttttgatg tagtccgcca tgtcagagtt tacctgacgc
    48901 gccagataaa taaagcgccc tttcgggttg gcgttaatgc gggcagaaag ctgatccagc
    48961 ggaatattga gcgcgttagc cagcgccttc cagcggtcac cgacgctgat accgccagcg
    49021 tcatgcactt ctttcgggtc agcccaaatc gcttttaccg gcacgctcac cgctaacggg
    49081 cgaccagaac ggtcagtaat catgccgcgg gaggtggaaa cttgctgaac gcgaagagaa
    49141 cgcatgtcgc cctctttcac cagcatatcc ggggagataa cttgtaacca cgctacgcgt
    49201 ccgagcagaa aagccagcgc caggagaata cagccgcata acaacgcaaa acgccaactg
    49261 ataaagttgg catgttcttc ctgacgtttt ggtttctgcg ttttcgccgc tgctttcatg
    49321 cgtcgcgttt atccttattt ttgcactacg atattttctt gtgacggatc aacatgctgc
    49381 atttgcagct tttccgtggc gatcctttcc acccggctat ggtcgccgag cgcattctct
    49441 tcaaggatca ggttgcgcca ttcaatgtct aaagcatctc gctccagcac cagttgttcg
    49501 cgctgagcgg tcagtaaacg ggtatggtgc gccgtggtta ccacggtcac cgccgtcaaa
    49561 ataatgcaaa tgaacaggca gagtggcagc ttcccaaatc gcaaaagatc gtcaccgata
    49621 acaccaggca atgcatggcg ctcgtggctt cccatcgatc ctttaacttt gcttagagct
    49681 tctgtcactc tgctgatcat gcgttcgtcc tctctgcaat acgcagaact gaactacggg
    49741 cacgagggtt ctcagccacc tcttcttcgc ccggcattaa cttgcctagt gctcgcagct
    49801 gacggccacc cagttttttg agctgctctt cagtcatcgg taaccctgcc ggaacttgcg
    49861 gaccgcggct gttttcacgc ataaaacgtt tcacaatacg gtcttccagc gagtggaagc
    49921 tgatgatcga aagccgccca cccggggcca gcacgttgag cgagcttttt agcgcctgct
    49981 ctatctcctc cagttcactg tttacccaaa tgcgcaccgc ctggaaggta cgggtcgcgg
    50041 gatgtttaaa cttatctttc accggcgttg cagcagccac gacttccgcc agttctttgg
    50101 tgcgggtcat cggctgttcg cggttacgct cgacaatggc gcgggcaatg cgtttggcaa
    50161 aatgctcttc accataggtt ttcaataccc aggcgatatc ggcttcttct gcggtttgta
    50221 gccattcagc ggctgactgc ccacgggttg ggtccatacg catgtccagc ggaccatcgc
    50281 gcataaagga aaagccgcgt tcagcatcat caagttgcgg tgaagagacg ccaagatcga
    50341 ggagaatgcc gtcgatcttg ccaataagat cgcgctcggc aacgtattcg cccagcgcgg
    50401 agaaaggtcc gtggatgatg gagaagcgcg gatcatcaat agtcttcgca acggcgatag
    50461 cctgcgggtc gcgatcgatc gccagcaaac gcccctcttc gccaagctgc gagaggatca
    50521 gacgtgagtg accaccgcga ccaaaagtcc catcaatgta gatgccatca ggacggatat
    50581 tgaggccatt aacggcttca tccagcagca ccgtagtatg tttatagttt tccatcattt
    50641 tatagagaca agtcctgcag tcgctccgat aagtctccgg tagccaactg ctctgcgtcg
    50701 atatcttcct tgacctgttg atgccaggtt gtttcatccc acagctcaaa cttgttgaac
    50761 tgtccaacca gcatcacttc ttttgtcagc ccggcatgtt gccgcagtac tggcgcgatt
    50821 aacaatcgac ctgcgccatc catctgacat tcgctggcat gacctaacag taggcgctgc
    50881 acacggcgct caaccgggtt catgctcaac agacgcgata atttttgctc gataatttcc
    50941 cattcaggca gggggtaaag cagcaggcac gggtgatgaa tgtcaatggt gcaaaccatt
    51001 tgaccggcag cgttctcaag cagctgttcc cgataacggg taggcactga taagcgccct
    51061 ttgctgtcga gattgactaa cgttgctccc cggaacatgc cagcctcacc ccttattccc
    51121 actttgcccc acaatttccc actgaaagga gtttacggag cggaggaaaa gcttgtcaag
    51181 ccagtcacaa ggcataccga ggtaaaaaac ccgatgttta cggctaatat cagcgttgtt
    51241 tattttacgt ccaacgtgcg aagcaaatta acgttatgaa tagctgtaag aaaaaatccc
    51301 tcatcgtcat cactgtttta aaatgactca ctattttcca cagcaaatat aaagtgtcag
    51361 tttgcgacgc gagcggcatt ttaggacata tcttccccgg ttaacagtcc ttgttacgtc
    51421 tgtgtggcgc ttgatgccag cgggtttgcg caaaagcaaa gaaaagtgtt tgttaaatcg
    51481 gcaattcatc gccgcatgta acaaaataat gcaaaaacgc gctgtcgata tcacttaatt
    51541 attgtgaatt tcagctggtg agttaattaa cgggcgggtt taaaaccatt tatcaataat
    51601 agtcaggaat agtcttattt actttaagga tattgat
//
LOCUS       DAZSIO010000009        49532 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZSIO010000009 DAZSIO010000000
VERSION     DAZSIO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.009"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..1150)
                     /gene="setB"
                     /locus_tag="ACHX5T_000441"
     CDS             complement(<1..1150)
                     /gene="setB"
                     /locus_tag="ACHX5T_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR8465781.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIAT"
     gene            1518..2648
                     /gene="fruB"
                     /locus_tag="ACHX5T_000442"
     CDS             1518..2648
                     /gene="fruB"
                     /locus_tag="ACHX5T_000442"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR8465782.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2648..3586
                     /gene="fruK"
                     /locus_tag="ACHX5T_000443"
     CDS             2648..3586
                     /gene="fruK"
                     /locus_tag="ACHX5T_000443"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR8465783.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3603..5294
                     /gene="fruA"
                     /locus_tag="ACHX5T_000444"
     CDS             3603..5294
                     /gene="fruA"
                     /locus_tag="ACHX5T_000444"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR8465784.1"
                     /translation="MKTLLIIDANLGQARAYMVKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5718..6659
                     /locus_tag="ACHX5T_000445"
     CDS             5718..6659
                     /locus_tag="ACHX5T_000445"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR8465785.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6647..7585
                     /gene="psuG"
                     /locus_tag="ACHX5T_000446"
     CDS             6647..7585
                     /gene="psuG"
                     /locus_tag="ACHX5T_000446"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR8465786.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7679..8929
                     /locus_tag="ACHX5T_000447"
     CDS             7679..8929
                     /locus_tag="ACHX5T_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR8465787.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9000..9694)
                     /locus_tag="ACHX5T_000448"
                     /pseudo
     CDS             complement(9000..9694)
                     /locus_tag="ACHX5T_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9825..10766
                     /gene="rihB"
                     /locus_tag="ACHX5T_000449"
     CDS             9825..10766
                     /gene="rihB"
                     /locus_tag="ACHX5T_000449"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR8465788.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10866..12116
                     /locus_tag="ACHX5T_000450"
     CDS             10866..12116
                     /locus_tag="ACHX5T_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR8465789.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12172..13260)
                     /locus_tag="ACHX5T_000451"
     CDS             complement(12172..13260)
                     /locus_tag="ACHX5T_000451"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR8465790.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="ACHX5T_000452"
     CDS             complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="ACHX5T_000452"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR8465791.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14194..15243)
                     /locus_tag="ACHX5T_000453"
     CDS             complement(14194..15243)
                     /locus_tag="ACHX5T_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR8465792.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15342..16223
                     /gene="yieE"
                     /locus_tag="ACHX5T_000454"
     CDS             15342..16223
                     /gene="yieE"
                     /locus_tag="ACHX5T_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR8465793.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16429..17898
                     /gene="lysP"
                     /locus_tag="ACHX5T_000455"
     CDS             16429..17898
                     /gene="lysP"
                     /locus_tag="ACHX5T_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR8465794.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18190..20181
                     /gene="cirA"
                     /locus_tag="ACHX5T_000456"
                     /pseudo
     CDS             18190..20181
                     /gene="cirA"
                     /locus_tag="ACHX5T_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="ACHX5T_000457"
                     /pseudo
     CDS             complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="ACHX5T_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21306..21974
                     /gene="folE"
                     /locus_tag="ACHX5T_000458"
     CDS             21306..21974
                     /gene="folE"
                     /locus_tag="ACHX5T_000458"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR8465795.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21991..23067
                     /gene="yeiB"
                     /locus_tag="ACHX5T_000459"
     CDS             21991..23067
                     /gene="yeiB"
                     /locus_tag="ACHX5T_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR8465796.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23209..24249
                     /gene="galS"
                     /locus_tag="ACHX5T_000460"
     CDS             23209..24249
                     /gene="galS"
                     /locus_tag="ACHX5T_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR8465797.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24529..25527
                     /gene="mglB"
                     /locus_tag="ACHX5T_000461"
     CDS             24529..25527
                     /gene="mglB"
                     /locus_tag="ACHX5T_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR8465798.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25588..27108
                     /gene="mglA"
                     /locus_tag="ACHX5T_000462"
     CDS             25588..27108
                     /gene="mglA"
                     /locus_tag="ACHX5T_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR8465799.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27124..28134
                     /gene="mglC"
                     /locus_tag="ACHX5T_000463"
     CDS             27124..28134
                     /gene="mglC"
                     /locus_tag="ACHX5T_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR8465800.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28170..29383)
                     /gene="preA"
                     /locus_tag="ACHX5T_000464"
                     /pseudo
     CDS             complement(28170..29383)
                     /gene="preA"
                     /locus_tag="ACHX5T_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29377..30615)
                     /gene="preT"
                     /locus_tag="ACHX5T_000465"
     CDS             complement(29377..30615)
                     /gene="preT"
                     /locus_tag="ACHX5T_000465"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFR8465801.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30809..31048)
                     /locus_tag="ACHX5T_000466"
     CDS             complement(30809..31048)
                     /locus_tag="ACHX5T_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR8465802.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="ACHX5T_000467"
     CDS             complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="ACHX5T_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR8465803.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31920..32804)
                     /gene="cdd"
                     /locus_tag="ACHX5T_000468"
     CDS             complement(31920..32804)
                     /gene="cdd"
                     /locus_tag="ACHX5T_000468"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR8465804.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32934..33629)
                     /locus_tag="ACHX5T_000469"
     CDS             complement(32934..33629)
                     /locus_tag="ACHX5T_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR8465805.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33626..34024)
                     /locus_tag="ACHX5T_000470"
     CDS             complement(33626..34024)
                     /locus_tag="ACHX5T_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR8465806.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34263..35210
                     /gene="dusC"
                     /locus_tag="ACHX5T_000471"
     CDS             34263..35210
                     /gene="dusC"
                     /locus_tag="ACHX5T_000471"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR8465807.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35581..35664)
                     /gene="yohP"
                     /locus_tag="ACHX5T_000472"
     CDS             complement(35581..35664)
                     /gene="yohP"
                     /locus_tag="ACHX5T_000472"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFR8465808.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35888..37324
                     /gene="mdtQ"
                     /locus_tag="ACHX5T_000473"
                     /pseudo
     CDS             35888..37324
                     /gene="mdtQ"
                     /locus_tag="ACHX5T_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37392..38138
                     /locus_tag="ACHX5T_000474"
                     /pseudo
     CDS             37392..38138
                     /locus_tag="ACHX5T_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013097944.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38255..38833)
                     /locus_tag="ACHX5T_000475"
     CDS             complement(38255..38833)
                     /locus_tag="ACHX5T_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR8465809.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39003..39590
                     /locus_tag="ACHX5T_000476"
     CDS             39003..39590
                     /locus_tag="ACHX5T_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR8465810.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39764..40696
                     /gene="pbpG"
                     /locus_tag="ACHX5T_000477"
     CDS             39764..40696
                     /gene="pbpG"
                     /locus_tag="ACHX5T_000477"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR8465811.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40734..42449)
                     /gene="dld"
                     /locus_tag="ACHX5T_000478"
     CDS             complement(40734..42449)
                     /gene="dld"
                     /locus_tag="ACHX5T_000478"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR8465812.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42645..44942
                     /gene="bglX"
                     /locus_tag="ACHX5T_000479"
     CDS             42645..44942
                     /gene="bglX"
                     /locus_tag="ACHX5T_000479"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR8465813.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45194..46111
                     /gene="osmF"
                     /locus_tag="ACHX5T_000480"
     CDS             45194..46111
                     /gene="osmF"
                     /locus_tag="ACHX5T_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR8465814.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46118..47275
                     /gene="yehY"
                     /locus_tag="ACHX5T_000481"
     CDS             46118..47275
                     /gene="yehY"
                     /locus_tag="ACHX5T_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFR8465815.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47268..48194
                     /gene="yehX"
                     /locus_tag="ACHX5T_000482"
     CDS             47268..48194
                     /gene="yehX"
                     /locus_tag="ACHX5T_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR8465816.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48199..48930
                     /gene="yehW"
                     /locus_tag="ACHX5T_000483"
     CDS             48199..48930
                     /gene="yehW"
                     /locus_tag="ACHX5T_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR8465817.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48911..49018)
                     /locus_tag="ACHX5T_000484"
     CDS             complement(48911..49018)
                     /locus_tag="ACHX5T_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR8465818.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49078..49532)
                     /locus_tag="ACHX5T_000485"
                     /pseudo
     CDS             complement(49078..49532)
                     /locus_tag="ACHX5T_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11955 a        12203 c        13311 g        12063 t
ORIGIN      
        1 gagtggcgat aatcatcacc atcgcaaacc agaacacagc gtgataattc cagatctcgg
       61 cgacgatgcc cgccactgat cctgcgatga tccagcccac gcgcgacgtg ttggtataga
      121 gcgtggtggc tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc atcccgatgc
      181 cgcccagaat gccaataaaa atagcattta gcagctgcaa gcccaacaga atggcaggtg
      241 aatgcgccat cagcattcct gcgtaaaaac agacgccacc cacggcagca acgcgcatta
      301 agaaacgctt acccagacgt ttggcgaaat atccggcaat caacatcgtc gggatttcca
      361 gcccggcggc ggtccccatc atcacaccgg ccagtttctc gggaagatgc agttcgttga
      421 taataaatag cggcatgttg atgatgtaca ggctgttcga gccccacatc aatgtacaaa
      481 tgacaaacag cagtagcgta tcgcgacggt tacggcgcgg cgcttcaacc gtgccggtcg
      541 ccagcggaag ctctttttgc atcgacggta aaaacagcca caccatcacg ccgcaaacaa
      601 taaacgctac cgctgcgctc agatacatta ccgtaaagct gaaacccatc gctaacgcat
      661 aagccagcgg tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa aaagagctga
      721 acatcaccgc ctcacgtccg gttttgtcgg catgttcacg ggcaagggca aacatttgcg
      781 ggttagcggt cgagccaaag ctgctaagaa agacgccaac gaatagcaaa acaaagtagt
      841 tgcgattcca agcaaaaagg gtgcaggcca gcacgcctaa caggcagcaa aagacaatca
      901 gcgatttgcg atcgccgcgc ttatcagagc gcccagcgag aaactgactt accagaatcc
      961 caatgacagc gctgccggta aagaagaatc ccaccatcgc cggacgggca tgtacttcat
     1021 cggtaaggaa aatactgagt gtcggggttt gcagagcacc cgcaataccg gtgagaaagg
     1081 caacgattaa aaacgccgtc gaggtcaggt caaacgattt cgcgctggag actgcggggg
     1141 agttatgcat gctggtcgat cactggaaga ggaagacgcg gagtttacgc tgcttgtcag
     1201 aaaataaaca gacagcgaaa tcaaaagcgc cacgaataat caaaatgcca tttcactgtt
     1261 gctgattggc tgctgacgct gctgaaatga aattgctgat aacggatttt cccatcagca
     1321 attaggaaaa atgacaaaaa attgtgcagc acatcaaact tttgctcata actttacggc
     1381 tttccttgcg tgctgaaaca gaagtattat gctttcttga aacgtttcag cgcgatcttg
     1441 tctttaaccc taagctaggt tggcgctttt tttctcatag aggctgaatc gtttcaattc
     1501 agcaagagag gagaactatg ttccagttat ccgtacagga catccatccg ggcgaaaagg
     1561 ccggagacaa agaagaggcg attcgccagg tcgctgcggc gctggtgcag gccggtaatg
     1621 tagcagaagg ctacgtcaat ggcatgctgg cgcgcgaaca gcaaacctca acgttcctcg
     1681 gcaatggtat tgctattcca cacggcacta ccgacacccg cgatcaggtg ctgaaaaccg
     1741 gcgttcaggt atttcagttc ccggaaggcg tcacctgggg tgacggtcag gtagcgtacg
     1801 tggcaatcgg tattgctgcc agctcggata aacatctggg cctgctacgc cagctgaccc
     1861 acgtactgag cgatgattcc gttgctgaac aactgaagtc agcaacaaca gcagaagaac
     1921 ttcgcgcatt actgatgggc gaaaagcaga gtgagcagct gaagctcgac aacgaaatgc
     1981 tgacgctgga tatcgtcgcc agcgatctgc tgactcttca ggcgctgaac gctgcgcgtc
     2041 tgaaagaggc gagggcagtt gacgccactt tcgtcaccaa agccatcaat gaacaaccgc
     2101 tgaatctcgg acagggtatc tggctgagcg atagcgccga aggcaatctg cgtagtgcga
     2161 ttgcggtaag ccgtgcggca aatgcttttg atgtggacgg cgaaacggca gccatgctgg
     2221 tgagtgtggc gatgaatgac gatcagccca tcgcggttct taagcgtctc gctgatttgt
     2281 tgctcgacaa taaagctgac cgcttgctga aagcggatgc ggcaacgttg ctggcgctgc
     2341 tgaccagcga tgatgcgccg accgacgacg tgttaagcgc ggagtttgtg gtgcgcaatg
     2401 aacacggcct gcatgctcgt ccaggtacca tgctggtcaa taccattaaa caatttaaca
     2461 gtgatattac cgtgacaaac cttgatggca ccggcaaacc ggcaaacgga cgtagtctga
     2521 tgaaagttgt ggcacttggc gttaagaaag gtcatcgcct acgctttacc gcccagggtg
     2581 cagatgctga acaggcgctg aaagccattg gcgacgctat cgctgctggt cttggggagg
     2641 gcgcataatg agcagacgtg ttgctacgat cacccttaat ccggcttatg accttgttgg
     2701 tttctgcccg gaaattgaac gcggcgaagt gaacctggtg aaaaccaccg gtctgcatgc
     2761 ggcgggtaaa ggcatcaacg tggccaaagt attaaaagac ctgggaattg atgtcaccgt
     2821 tggcggcttc ctgggtaaag acaatcagga tggttttcag caactgttca gcgagctggg
     2881 cattgccaac cgtttccagg ttgtacaggg gcgcacccga attaacgtta agctgacgga
     2941 aaaagacggc gaagtgaccg acttcaactt ctcgggtttc gaagtcaccc ccgccgactg
     3001 ggaacgcttt gtgactgatt ctctgagctg gctcggtcag ttcgatatgg tctgtgtcag
     3061 cggaagctta ccgtcaggcg tcagcccgga agcgttcacc gactggatga ctcgcctgcg
     3121 tagtcagtgt ccttgcatta tctttgatag tagccgtgaa gcgttagtag caggtttgaa
     3181 agcggcaccg tggctggtga aacctaaccg ccgcgagttg gaaatctggg caggccgtaa
     3241 actgcctgaa atgaaagatg tgattgaagc tgcacatgcg ctacgtgaac aaggtattgc
     3301 gcatgttgtt atttcactgg gtgccgaagg cgcgctttgg gttaatgcct ccggcgaatg
     3361 gatcgctaaa ccaccgtcag tcgatgtcgt aagcaccgtt ggcgcagggg attctatggt
     3421 tggtggcctg atttatggct tgctgatgcg tgaatccagt gaacacacac tgcgtctggc
     3481 gacagctgtt gcagccctgg cggtaagtca aagcaatgtg ggtattaccg atcgtccgca
     3541 gttggccgca atgatggcgc gcgtcgactt acaacctttt aactgacagc aggagaggca
     3601 taatgaaaac gctgctgatt attgacgcta atctcggtca ggcacgcgcc tatatggtga
     3661 agaccctgct gggcgcggcg gcgcgaaaag caaaactgga aatcatcgac aatccgaacg
     3721 acgcagaaat ggcgattgtt ctcggtgatt ccatcccgaa tgacagcgcg ctgaacggta
     3781 aaaatgtctg gctgggtgat atttcccggg cagttgcgca ccctgagctg ttcctgagtg
     3841 aagccaaagg ccatgcgaaa ccttacactg cgccggtcac tgcgacagca ccggttgccg
     3901 ccagcggtcc gaaacgcgta gttgcggtga ctgcttgccc gactggcgta gcacacacct
     3961 ttatggcggc tgaagccatt gaaaccgaag cgaaaaaacg tggctggtgg gtgaaagttg
     4021 aaacccgtgg ttctgttggc gcgggtaatg caatcactcc cgaggaagta gccgcagcgg
     4081 atctggtgat tgtggcggca gatatcgaag tggatctggc gaaatttgct ggtaaaccga
     4141 tgtatcgtac ctctaccggt ctggcgctga agaaaacctc gcaggaactg gataaagcgg
     4201 ttgctgaagc aacgccgtat gaaccggcgg gcaaagctca aacggcgacc actgaaggta
     4261 agaaagagag tgcaggcgct tatcgtcact tgctaacggg cgtctcttac atgctgccga
     4321 tggtcgttgc tggtggtctg tgtatcgcgc tttcttttgc ttttggtatc gaagcgttta
     4381 aagagccggg tacgttggca gcggcgctga tgcagattgg tggtggttca gcctttgcgc
     4441 tgatggtgcc ggtactggca ggttatattg ccttctccat tgccgatcgt ccgggcctca
     4501 cgccgggtct gattggcggt atgctggcgg tcagcaccgg ttctggcttc attggcggta
     4561 ttattgcggg cttcctggct ggttacattg cgaagttaat cagtacgcaa ctgaaactgc
     4621 cgcagagtat ggaggcgctg aaaccgatcc tgatcattcc gctaatttcc agtctggtgg
     4681 tcggtctggc gatgatctac ctgatcggta aaccagttgc tggcattctc gaagggctga
     4741 ctcactggct gcagaccatg gggactgcga atgcggttct gctgggggcg atcctcggtg
     4801 gcatgatgtg tactgacatg ggcggtccgg taaacaaagc agcgtacgca ttcggtgtgg
     4861 gtctgctgag tactcaaacc tatggcccga tggcggcgat tatggcggca ggtatggtgc
     4921 caccgctggc aatgggcctg gcaacaatgg tggcgcgtcg caaattcgac aaagcgcagc
     4981 aggaaggtgg caaagccgct ctggtattgg gactgtgctt catttcggaa ggtgcaattc
     5041 cgtttgctgc tcgtgatccg atgcgtgtgc tgccgtgctg tatcgtgggt ggggcgctga
     5101 ctggcgcaat ctcaatggcg attggtgcga aactgatggc accgcacggt ggtctgtttg
     5161 ttctgctgat ccctggcgct attacgccgg tattgggtta cctggtagca attattgccg
     5221 gtacgctggt ggcgggtttg gcctatgcct tcctgaaacg tccggaagtg gacgcagtag
     5281 cgaaagcagc gtaataaaag gtgtgttaca gggcagaaat ttcctgccct gtaacatttg
     5341 atgagcgcaa agagaatgac cagcttgtga atgtaatttt atgaatttaa atggttttat
     5401 ctttcagtag aaacttcaaa cgttcatcaa acactaaggt tatttcctca agggcagatt
     5461 attttctgcc cttttttagt gttcctgttc tgtgcatccc gtcacaaatt cctcgctttt
     5521 gtaattcagg catttaatcg cattttgtga gcctgctcaa atctgcccat tttgcctctc
     5581 tggcggtaat ttactttacg taaaacatgc atactatgag cacatgttta aatatgaaca
     5641 catgtttaaa ccgtctttgc agtatctata ttcttgtgga ttgacagggt tggtaatttt
     5701 ttaccagagg acgtgctatg cgcgaaaagg attatgtcgt aattataggt tcggcgaata
     5761 ttgatgtcgc cggatattca catgaatcat taaattatgc ggattcaaat ccaggtaaaa
     5821 taaaatttac gcctggtgga gtagggcgca atattgcaca aaacctggcg ttgctgggta
     5881 acaaagcctg gctactgagc gccgtaggca gtgattttta tggtcaatcg ctgctaacgc
     5941 aaaccaatca atctggtgtt tatgtcgata aatgcctgat tgtgccggga gaaaatacgt
     6001 cgagttattt atcattactc gataataccg gtgaaatgct ggttgctata aatgacatga
     6061 atattagcaa cgctattaca gctgaatatc tcgcacagca ccgtgaattt attcagaggg
     6121 caaaggtcat tgtcgcggac tgtaatatca gtgaagaggc actggcatgg attctggata
     6181 atgccgccaa cgtacccgta tttgtcgatc cggtttcagc gtggaaatgt gtcaaagtgc
     6241 gcgaccgtct aaatcagaac cacactctga agccaaaccg ccttgaagcg gaaaccctga
     6301 gtgggattgc gctgtcaggg cgtgacgatg tggcaaaagt tgctgcctgg ttccatcaac
     6361 atggcctgaa ccgactggta ttgagcatgg gcggcgacgg cgtttattac agcgatatca
     6421 gcggtgaaaa tggctggtct gcgccgatca aaaccaatgt tattaatgtt accggagcgg
     6481 gcgatgccat gatggcggga cttgcttcgt gttgggtaga cggaatgccg tttgccgaat
     6541 ctgttcgttt cgcacaggga tgttcgtcaa tggcgctctc ctgtgaatac accaataacc
     6601 ccgatttatc gattgccaac gttatatcgt tagtggagaa cgcagaatgt ctgaattaaa
     6661 aatttcccct gaattattac aaatttctcc ggaagtgcag gacgctttaa aaaacaaaaa
     6721 accggttgtg gcgctggaat cgaccattat ttctcacggg atgccgttcc cacaaaatgc
     6781 ccagaccgca attgaagtag aagaaactat tcgtaaacag ggcgcagtac ctgccactat
     6841 cgccattatt ggcggcgtga tgaaagtggg tttaagcaaa gaaaaaattg aattactggg
     6901 tcgtgaaggg cataacgtga ccaaagttag tcgtcgcgat ttaccttttg ttgttgccgc
     6961 cggaaaaaat ggcgcaacca ctgtggcttc aacgatgatt attgcggcgc ttgccggaat
     7021 taaagtattt gccaccgggg gaattggtgg tgtgcatcgc ggggcggaac ataccttcga
     7081 tatttctgcc gatttgcaag aactggcaaa tactaatgtc accgttgttt gtgccggggc
     7141 gaaatctatt ctcgatttag gattaaccac tgagtattta gaaaccttcg gtgtgccgtt
     7201 aattggctat cagactaaag cgctgcctgc gtttttctgc cgtaccagct cgtttgacgt
     7261 cagcattcgt ctcgacagcg ccagtgaaat tgcccgtgca atggcggtga aatggcaaag
     7321 cgggctgaac ggtggcctcg tggtagcgaa cccgatcccg gaacagtttg cgatgccgga
     7381 ggaatctatc aatgcagcca tagatcaagc cgtcgccgaa gccgaagagc agggcgttat
     7441 tggtaaagaa agtacaccgt tcctgctggc gcgcgttgct gagctgacca gcggtgacag
     7501 cctgaaatcc aatatccagc tggtgttcaa caacgccatt ctggcgagtg aaattgccaa
     7561 agaataccag cgtctcgcgg gttaatttct gacgggcaga aagccgtcgc ccgtactcat
     7621 caagcatacc aaaacctggc gctgacgccg ggtttactgg catgaaggga aaatcattat
     7681 ggatataatg agaagtgttg tggggatggt ggtgttactg gcaatagcat ttctgttgtc
     7741 agtgaataaa aagagcatca gtttgcgcac ggttggagcc gcactgctgc tgcaaatcgc
     7801 tattggtggc atcatgctct acttcccacc gggaaaatgg gcagtagaac aggcggcatt
     7861 aggcgttcat aaagtgatgt cttacagtga tgccggtagc gccttcattt ttggttcgct
     7921 ggttgggccg aaaatggatg tcctgtttga cggtgcgggt tttatcttcg cctttcgcgt
     7981 acttccggcg attattttcg ttactgcgct catcagtctg ctgtactaca ttggcgtgat
     8041 ggggctgctg attcgcatcc ttggcagcat tttccagaaa gccctcaaca tcagcaaaat
     8101 cgaatctttt gttgcggtta ccactatttt cctcgggcaa aatgagatcc cggcgatcgt
     8161 taaaccgttt atcgatcgcg tgaatcgcaa cgagttgttt accgcaattt gtagcgggat
     8221 ggcgtccatt gctggttcgg tgatgattgg ttatgccgga atgggcgtac caattgacta
     8281 cctgttagcg gcatcgctga tggcgatccc cggcggtatt ttgtttgcac gtattcttag
     8341 cccggcaacc gagccttcgc aggtcacatt tgaaaatctg tcgttcagcg aaacgccgcc
     8401 aaaaagcttt atcgaagcgg cggcgagcgg tgcgatgacc gggctgaaaa tcgccgctgg
     8461 tgtagcgacg gtggtaatgg cgtttgtcgc aattattgcg ctgatcaacg gcattatcgg
     8521 cggaattggc ggctggtttg gtttcgccaa tgcctctctg gaaagtattt ttggctatgt
     8581 gctggcaccg ctggcgtgga tcatgggtgt ggactggagt gatgccaacc ttgctggtag
     8641 cctgattggg cagaaactgg cgattaacga attcgtcgct tacctgagtt tctccccata
     8701 cctgcaaacg ggcggcacgc tggaagtgaa aaccgtggcg attatatcct ttgcgctttg
     8761 tggttttgct aactttggtt ctatcggtgt tgtcgttggc gcattttcgg ctatttcgcc
     8821 aaaacgtgct ccagaaatcg cccagcttgg tttacgggcg ctggcagcag caacgctttc
     8881 caacctgatg agtgcgacta ttgccgggtt ctttattggt ctggcgtaat gctcgatttg
     8941 tagaccggat aaaacgtatt aagcgttgca tccggcaatc gcgctattgc aaataatttt
     9001 tactgcatca tcccggagaa tttattctcc gggtccatct ccagcgccag tcctgacaac
     9061 tgctttctgt ttttaattag atatcctttc ttttaattaa taaaccgtcg tgaatgaatt
     9121 gcgcgagaac atataaaaga tgtcgataag aaacgcctaa atactctgcc gcttgcgtat
     9181 gcttttcgtg ataaagatca ccttcttgtg agagtaatat aaatgctgcc aggcgattca
     9241 ctaacggaaa tgattgattc tgagttaaag aaacaatatt acgataattt ttatgactta
     9301 aggtgacgca aagttttcgt aaaaataggg tgtcgtttaa taacagcgga cggtaatgtt
     9361 tcatagggag cgcaaggcac caacactctt caatagcctg caccgcacgc ggttcatggt
     9421 ctttgtcgat taactcaatc tcgccaataa aacagggagc ggcaaagaaa tcgatcagcg
     9481 acacgcgacc attggcaagc gtggcgtaaa gcctggcgcg gcctcgcgtc agataaaaca
     9541 gccatgacgg ctgttgccct tcctgcacaa tgtagtcacg cgctaaaaaa tgaaacagcc
     9601 gcgtatcggc tgaaacatcc gttgaaaagc aatccttaaa cgcagattca ctcatcaatt
     9661 gctgcttaag gtcattattg tggatttctt tcacgatgtt tatccgctat gagatttctc
     9721 atatttagca tacgtacgct gttgttaaga ttcaatcatt ggcccggtcg aattttcttt
     9781 gctgtagaaa aaaataacgc aactggaaac agaggaaata aacaatggaa aagagaaaaa
     9841 ttattctgga ttgtgagccg ggtcatgatg atgctattgc aatgatgatg gcagcgaaac
     9901 atccggcaat agatttatta ggcatcacta ttgtagcggg taatcagacg cttgataaaa
     9961 cattaattaa tggcctgaat gtttgccaga aactggagat taatgttccg gtttatgcgg
    10021 ggatgccgca acccattatg cgtaaacaaa tcgttgccga taatattcac ggtgaaaccg
    10081 gactggatgg cccggtattc gagccgctga cccgccaggc agaaagtacc catgcggtga
    10141 aatatatcat cgataccctg atggcaagcg atggcgatat cactctggtg ccggttggtc
    10201 cgctttcaaa tatcgcggtg gcaatgcgta tgcaacccgc aatcctgccc aaaatccgtg
    10261 aaattgtgct aatgggcggc gcttacggta caggcaactt caccccatct gccgagttca
    10321 atatctttgc cgacccggaa gccgcccgcg tggtgttcac ctccggcgtt ccattagtga
    10381 tgatgggcct cgatcttacc aaccagaccg tttgcacccc ggatgtaatt gctcggatgg
    10441 aaagggtagg cggccctgcc ggagagctgt tcagcgacat catgaacttc actctcaaaa
    10501 cgcagttcga atactacggc ctggctggcg gcccggtgca tgacgccacc tgcattggtt
    10561 atctgatcaa ccctgatggc attaaaaccc aggatatgta cgtcgaagtg gacgtcaaca
    10621 gcggcccttg ctacgggcgt acggtctgcg acgaactggg cgttcttggc aagcccgcta
    10681 ataccaaagt cggcatcact attgataccg actggttctg gggcttagtc gaagagtgcg
    10741 tgcgcggcta catcaaaacc cattaagtgt tgataacaag ccgggacagt atcgcaagat
    10801 gcaggcccgg ctatttctga tgggcagaaa gccgtcgccc aaaatgacat gaagagataa
    10861 tcactatgga tgtcatgaga agtgttctgg gaatggtggt actgctggca attgcgtttt
    10921 tactgtcagt aaacaagaag aagatcagcc tgcgtaccgt tggcgcggcg ttagtgttac
    10981 aggtcgtgat tggcgggatt atgctttggt taccgccagg gcgttgggtc gcagaaaaag
    11041 tcgcttttgg cgtccacaaa gtgatggcgt acagcgacgc aggtagcgca tttatcttcg
    11101 gttctctggt cggaccgaaa atggatacct tatttgatgg cgcaggattt atctttggtt
    11161 tcagggtatt accggcaatt atcttcgtca ctgcactggt gagtattctc tactacatcg
    11221 gtgtgatggg gatcttaatt cgcattctcg gcggtatctt ccagaaagct ttaaatatca
    11281 gcaagatcga gtcattcgtc gcggtcacca ccattttcct cgggcaaaac gaaattccgg
    11341 cgatcgtcaa accctttatc gatcgtctga atcgcaatga attatttaca gcgatttgta
    11401 gtggcatggc ctcgattgct ggttcgacaa tgattggtta cgccgcactg ggcgtacctg
    11461 tggaatattt gctggcggca tcgttaatgg cgatccctgg cgggatcttg tttgcccgcc
    11521 tgttaagccc ggctacggaa tcttcgcagg tttcttttaa taacctctct ttcaccgaaa
    11581 caccgccaaa aagcattatt gaagccgatg cgacaggggc aatgaccggg ctgaaaatcg
    11641 ccgccggtgt agcgacagtt gttatggcat ttgtcgccat cattgcgtta attaacggta
    11701 ttatcggcgg cgttggcggc tggtttggtt ttgaacatgc ctcgctggag tccattttag
    11761 gttacctgct ggccccactt gcgtgggtaa tgggtgttga ctggagtgat gcaaatcttg
    11821 ccgggagttt gattggacag aaactggcaa taaatgaatt tgtcgcttat ctcaatttct
    11881 caccctatct gcaaacggct ggcactctgg atgctaaaac cgtggcgatt atttccttcg
    11941 cgttgtgcgg tttcgctaac tttggttcta tcggggtggt ggtgggggcg ttttctgcgg
    12001 ttgcgccaca ccgtgcgccg gaaatcgccc agcttggttt acgcgcgctg gcggcggcga
    12061 cactttctaa tctgatgagt gccaccattg ccgggttctt tattggttta gcgtagtgtg
    12121 caatttatga gccggattag atgcggtcaa gcgtcgaatc cggcaacatt gttacaccat
    12181 tggcactaat gacagcgcgt tatcggcaga cagggtaggg ttgtttaagc tgccgctggc
    12241 gcgtgaaatt gccgcgcagg ccatcgcaaa acgggcgctg tcacggaaat tgtttccctc
    12301 cagaaagcta tataccagac ccgccataaa accatcgtca gcaccaaaac tgtctaccgt
    12361 cgtatgcgct ggcacagtca gcaaaaattg ttcaccatcc ttttcgctgc aataaactga
    12421 ctcatcgggc aaataaacaa acagttgctg aacaccttgc tgatgcaatg cattcactgc
    12481 ggcattacgg tcagcatcgc tggtgatcgc ctgtccccat aaaatttcca gctccggtaa
    12541 agtgggtttc agggtgtgaa tatgcgccag ccagtgtttg attttgcccg ctttgaattc
    12601 tgaaacggta tcgacaaaca ccgggatctc atcagcgagg gtaaagaccc attccagcgc
    12661 ctcggctgtc aggttgcaat cagccagtac cacgcccgca tgacgaagta aatcgcgcga
    12721 cccgttcagt agttgcggtg tcaactgttc cagcagatgg gtatcgttaa ttgccagcac
    12781 ggtttgatcg tctcgattgg caattgccag atacgtcgat gtgctttgac catgcaaacg
    12841 aacgcagccg gagacattca cgccggcacg gcgcgtttct tccaggagca tttcgccata
    12901 aaagtcatcg ccaatcactg aaagcaaatg gacgtcacgg cctaacagcg ccagattgtg
    12961 ggcgatattg cgtccaacgc cgcctgccga acaatgaatt gtaccgggat gagaagccgc
    13021 ttgcgggtaa tggatatccg ccatcccgcg aatatccata ttgattgtcc ccaccactac
    13081 gcagtattcc tgctcggtga gaatgtaacc tttgtcttta atccggcctt tgcgcattaa
    13141 atccataata tgcgccgcaa cgcgcgaacg actgatttgc agcatgtccg caatttcgtt
    13201 ctgctgaatc agcgggttac gccgtaaaat tgcaaggatc tccttttccc ggttatttat
    13261 cttcaggcta ccgctttttc agtttgttgc gctttcagcc aggcgatctc ttctgcccag
    13321 atatccgggt tgatggtttc aaggatcagc ggaatgccgt cgaaacggtc gttctgcata
    13381 atccagcgga acgcatcatg accgatattg ccttcaccga ggctatgatg gcggtcaacg
    13441 cggctgccaa aggtactttt cgcatcgtta aggtgcatcc cgcgcaaata cttaaagccg
    13501 acaatacggg caaaatcagc gaacgttttt tcgcattcgg ctggagtacg caaatcatac
    13561 ccggcagcga aagcatggca ggtatcaatg cagacgccga cgcgggattt atcttccacg
    13621 ccgtcgataa tcgccgcgag atgttcgaat ttaaacccta agttactgcc ctgaccggcg
    13681 gtgttttcaa tcaccgctgt tacaccttga gttttatcca gcgcaatgtt gatggattcg
    13741 gcaatacgcg caaggcaatc ctcttctgaa atctgcatca gatggctgcc agggtggaag
    13801 ttgagcaaag aaagccccag ttgttcgcaa cgctgcattt catctataaa ggcatcgcgc
    13861 gatttttcca gagcttcagt gaccggatgt ccgaggttga tcagataact gtcgtgggga
    13921 agaatttgcg ccgatgtgta gtgatatttt tcacaggcgg ctttgaattc atcgatggtt
    13981 tgcgtcgtga gcggtgcggc acgccactga cgttggtttt tggtgaacaa ggcaaacgcg
    14041 gttgcgtcga tttcggcggc gcgaattgcg gcatttgcca gaccgccagc agcactaacg
    14101 tgcgctccaa tgtatttcat gcgaggactc ctgttaaacc cgctggagga aaacggtaat
    14161 gatagcgggt taacaggagc aagaagtagt ggtttatgcg atgacgcttt gaatcacata
    14221 gttaatcgca ccaccaccaa caatcagcca ggcaaacagt accagtgcca tcagcagagg
    14281 tttcgcccca gcttttttca gcgcgctgac gtgagtggtc agacccagcg ccgccatcgc
    14341 cattgccagc aggaaggtat ccagcgttac cagcatgttc accacgctct gcggtaacag
    14401 gtggaacgag ttaaagatgg caactacgat gaacaagatg gcaaaccacg gaatagtgat
    14461 tttgcttttc tcgccgctgt tcgccccaga cagctgttta acacgcgccg ccagcaggat
    14521 gaggaacgga gccagcatca tcacgcgcag cattttggaa ataactgctg cgttttctgc
    14581 atccgggctg atggcatgac ctgccgccac cacctgcgcc acttcgtgca cagtagaacc
    14641 aatgtagata ccgaaagttt ccggactaaa ccattgagac atcagcggat atatcgccgg
    14701 gtagaggaaa atcgcgacgg tcccgaagat aacaacggtt gcaacagcca cggttacttt
    14761 actggcttcc gctttcacta ccggctcagt cgccagtacc gcggcagcac cacagatact
    14821 gctaccggca ccgatcaacc aactggtgtg cttatccaga ccaaacactt tctgccccag
    14881 gaagcaagcc agcaggaagg tactggacag cgtcagcacg tcaatgatga tcccactgat
    14941 accgacatcg gcaatttgcg agaacgtcag acggaagcca taaagaatga tacccagacg
    15001 taataaatat tgcttggcaa acagcacacc accgtcacag cttttccaga tgtgcggata
    15061 gatggtgttg cctaaaacca tccccaacaa gattgcgagg gtgagggcac taaacccggc
    15121 acccgcaacc gcgggaatgg aaccacccca cagggcgacc ccggtgataa ctgcactcag
    15181 ggctaacccc ggaataaaat gccacagtgt acgatgttgt ttctgtaagg tgatattcgt
    15241 cataaccctc tcctttaacc gggcataagg ttacgactaa ctggtttaaa aataaaattg
    15301 attatatatt tataattaat ctttataagt ggtaagcgac tatgcacatc accctccggc
    15361 agttggaagt ttttgcagaa gtattgaaaa gtggatcaac cacccaggcg tcggtgatgc
    15421 tggcgttgtc gcaatcagca gtgagcgcag ccttgactga cctggaaggg cagcttggcg
    15481 ttcaactgtt tgatcgcgtg gggaaaagac tggttgttaa tgaacacggg cggctgctct
    15541 atccgcgagc gttggcactg cttgaacagg cggttgaaat cgaacaactg tttcgcgaag
    15601 acaacggcgc gattcgtatc tatgccagta gtaccatcgg taactacatt ctgcctgcag
    15661 ttatcgcccg ttatcgccat gattatccgc agttgccgat tgaacttagc gttgggaaca
    15721 gccaggacgt gatgcaagcg gtgctggatt tccgcgttga tattggcttt attgaaggac
    15781 cgtgccacag cactgaaatc atttctgaac cgtggctgga agacgagctg gtggttttcg
    15841 ccgcgccgac ttcgccgttg gctcgtggtc cggtcacctt agaacagctg gccgctgcgc
    15901 cgtggatcct gcgtgaacgc ggttccggca cgcgggatat tgtcgattat ctgttgctgt
    15961 cacatttacc gaagtttgag atggcgatgg aattaggtaa ctccgaggca atcaaacatg
    16021 cggtgcgtca tgggttggga attagttgcc tgtcgcgacg tgtgattgaa gatcaattgc
    16081 aggcaggcac attaagtgaa gttgcggtcc ctctgccgcg cctgatgcgt acgttgtggc
    16141 gtatacatca tcggcaaaaa cacctttcca acgcgctacg gcgctttctg gactattgcg
    16201 atcccgcaaa tgtgccgcgt taagttgctg tacaagaaca tgctggtgct gtgtcgattt
    16261 cgtgacgcag cgccttcagc atgcattcgc cagaaaagag attggctgct ttacttataa
    16321 tccctgggcg atcatgaagg tgtcttataa ccgtgtattt ctgccggaag gattgccaat
    16381 cgtctgctac aatcgcgcca tcatttttaa gatggatagc atttttgtat gggttccgaa
    16441 actaaaacta cagaagcgcc gggcttacgc cgtgaattaa aggcgcgtca cctgacgatg
    16501 attgccattg gcggttccat cggtacaggt ctttttgttg cctctggcgc aacgatttct
    16561 caggcaggtc cgggcggggc attgctctcg tatatgctga ttggcctgat ggtttacttc
    16621 ctgatgacca gtctcggtga attggctgca tatatgccgg tttccggttc gtttgccact
    16681 tacggtcaga actatgttga agaaggcttt ggcttcgcgc tgggctggaa ctactggtac
    16741 aactgggcgg tgactattgc cgttgacctg gttgcagcac agctggtcat gagctggtgg
    16801 ttcccggata caccgggctg gatctggagt gcgttgttcc tcggcgttat cttcctgttg
    16861 aactacatct ctgttcgcgg ctttggtgaa gcggaatact ggttctcact gatcaaagtc
    16921 acgacagtta ttgtctttat catcgttggt gtgctgatga ttatcggtat cttcaaaggc
    16981 gcgcagcctg cgggctggag caactggaca atcggcgaag cgccgtttgc tggtggtttt
    17041 gcggcgatga tcggcgtagc tatgattgtc ggcttctctt tccagggaac cgagctgatc
    17101 ggtattgctg caggcgagtc cgaagatccg gcgaaaaaca ttccacgcgc ggtacgtcag
    17161 gtattctggc gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag cctgattatt
    17221 ccgtataccg atccgagcct gctgcgtaac gatgttaaag acatcagcgt cagtccgttc
    17281 accctggtgt tccagcacgc gggtctgctc tctgcggcgg cggtgatgaa cgcagttatt
    17341 ctgacggcgg tgctgtcagc aggtaactcc ggtatgtatg cgtctactcg tatgctgtac
    17401 accctggcgt gtgacggtaa agcgccgcgc attttcgcta aattgtcgcg tggtggcgtg
    17461 ccgcgtaatg cgctgtatgc gacgacggtg attgccggtt tgtgcttcct gacctccatg
    17521 tttggcaacc agacggtata cctgtggctg ctgaacacct ccgggatgac gggttttatc
    17581 gcctggctgg ggattgccat tagccactat cgcttccgtc gcggttacgt attgcaggga
    17641 cacgacatta acgatctgcc gtaccgttca ggtttcttcc cactggggcc gatcttcgca
    17701 ttcattctgt gtctgattat cactttgggc cagaactacg aagcgttcct gaaagatacc
    17761 attgactggg gcggcgtagc ggcaacgtat attggtatcc cgctgttcct gattatttgg
    17821 ttcggctaca agctgattaa aggaactcac ttcgtacgct acagcgaaat gaagttcccg
    17881 cagaacgata agaaataagt ttcctccctt ccttgctaag ccctctcaac cgagagggct
    17941 ttttcaattc catttccctg acaaatcatg cggatataaa atttaacatt tggattgata
    18001 attgttatcg tttgcattat cgttacgacg caatcaaaaa aggctgacaa atcagaggct
    18061 gttccggctt tctgggatga tcacctgcat acaaataagt ccaccgcgat gctgccgtac
    18121 gcaaggggac gtaaagaaga tgtgagcgat acccatttta ttttcgtagt tacctcatgg
    18181 agatatggaa tgtttaggtt gaaccctttc gtacgggtcg ggctgtgttt gtccgctatt
    18241 tcttgtgcat ggcctgtgtt agcggtcgat gatgatggcg aaacgatggt tgtcactgca
    18301 tcttccgtgg aacaaaatct taaagatgca cccgccagta tcagcgtcat tacccaggaa
    18361 gacctgcagc gaaaaccggt acagaatctg aaggatgtcc tcaaagaagt gcctggcgta
    18421 caactgacga acgaagggga taaccgtaag ggcgttagta ttcgtggtct ggacagcagc
    18481 tacaccctga ttcttgtcga cggtaaacgc gttaactccc gcaatgccgt cttccgccac
    18541 aatgatttcg atctgaactg gatcccggtc gattccatcg aacgtattga agtggttcgt
    18601 ggcccgatgt cgtcgctata cggctccgat gcgctcggcg gtgtagtgaa tatcatcacc
    18661 aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg ataccaccat tcaggaacat
    18721 cgcgatcgcg gtgataccta taacggtcag ttctttacca gtggaccatt aattgatggt
    18781 gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg aaaaggatga cccgcaaaac
    18841 tcaacgacca ccgataccgg agaaacgccg cgtattgaag gattctccag ccgcgacggc
    18901 aatgtcgaat ttgcctagac accgaatcaa aatcacgatt ttactgccgg atacggtttc
    18961 gaccgtcagg atcgtgattc cgattcgctg gacaaaaacc gcctggaacg ccagaactac
    19021 tgcgtcagcc ataatgggcg ttgggattac ggcaccagcg aactgaaatc ctacggtgag
    19081 aaagtcgaga acaaaaaccc tggcaacagc agcccgataa cttccgaaag caatacggtc
    19141 gacggcaaat acacgttgcc gctgacggcg attaatcagt ttctcacggt tggcggtgaa
    19201 tggcgtcacg acaaacttag cgatgcggtg aacctgaccg ggggaaccag ctccaaaacg
    19261 tctgccagcc agtacgcgct gtttgtggaa gatgaatggc ggatcttcga gccgctggcg
    19321 ctgacgaccg gcgtgcgtat ggacgatcac gaaacctacg gtgaacactg gagtccgcgt
    19381 gcctacatgg tttataacgc caccgacacc gtaacggtga aagggggctg ggcgacggca
    19441 tttaaagcac cttctctgtt gcaacttagc cctgactgga cgagcaattc ctgccgtggc
    19501 gcatgtaaga ttgtgggtag cccggatctg aaaccagaaa ccagcgaaag ttgggagctg
    19561 gggctttact acatgggtga agaaggctgg ctggaagggg gtgaatccag cgttatcgtt
    19621 ttccgtaacg atgtgaaaga tcgtatcagc atcagccgta cgtctgacgt caacgctgca
    19681 ccgggctacc aaaactttgt tggttttgag acgggcgcta acggacggcg cataccggta
    19741 tttagctact acagcgttaa caaagctcgt attcagggcg tggaaaccga actgaaaatt
    19801 ccgttcaacg atgaatggaa actgtcgatc aactacacct acaacgatgg tcgtgatgtc
    19861 agcaacggcg aaaacaaacc gctatccgat ctgccgttcc atactgctaa cggtacgctg
    19921 gactggaaac cgctggcgct ggaagactgg tcattctatg tttctggtca ctataccggg
    19981 cagaaacgcg ccgacagcgc gacggctaaa acaccgggcg gttataccat ctggaatacc
    20041 ggcgcggcct ggcaggtgac taaagacgtc aaactgcgcg caggcgtgct gaaccttggc
    20101 gacaaggatc tcagtcgtga cgactacagc tataacgaag acggacgtcg ttactttatg
    20161 gcagtggatt atcgcttctg atgagaagat gcccggcgaa ccgggcggac tttcacttca
    20221 gtaaatactg cgcatggaag cgcaggtgat cctctataaa agaggcgatg aagtagtaac
    20281 tgtgatcata tcccggctga atacgcagcg tcatcggcca cgctttctgg cgcgcggctt
    20341 ctgccaatac cgcaggttgc aactggtcgg caagaaactg atcattatcg ccctgatcga
    20401 taagcgtcgg gatcgcatcc tgcgcgttac tggcatacat cagcgcgcaa ctatcccatt
    20461 ccagcatgca tttttgtcct cacctaaata ggtgctaaac gctttgattc cccacgggac
    20521 gctgcacgga ttcacaatgg gcgcaaaggc cgaaacgctg gtgtatttac ccggattttt
    20581 cagcgccata atcagcgcac cgtgaccacc cattgagtga ccgctaatgg cgcagcggtc
    20641 gctgacatta aattgcgact gaaccagcgc cggtaattca tcgcgcagat aatcatacat
    20701 ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg cgccctggcc
    20761 taaatcgtag ccatcgtcgt tggcaacctt ttcgccgcgc gggctggtgt ctggcatcac
    20821 cagtacaatc cccagttccg ccgctacccg ctgggcaccc gccttggtgg tgaagttctc
    20881 gtcattgcag gttaatccgg aaagccagta cagcactggt ggcggggtgt gatcacgagg
    20941 gggggggaga aagatactga acgtcatcgg gcagtttaag gtactggagt cgtgtcgcca
    21001 gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc atttccatgc gtggctcctt
    21061 gttgtgttgt ttgcaatttc ttcatcatac cgtttattca atgtgctgtg agtaactttc
    21121 acttccgtat ttgcataacg atgttttaac atctgctgat gaaaggcagc ggcaattaca
    21181 ataattatcg ctgtgaatac tggattatgt gcgccgcctc acgcacaata atcaagctgt
    21241 aaatcagctt aataactttg cccccacgca gggtggaggc gtcacacctg caggagaaat
    21301 cataaatgcc atcactcagt aaagaagcgg ccctggttca tgaagcgtta gttgcgagag
    21361 gactggaaac accgctgcgc ccgcccgtgc atgaaatgga taacgaaacg cgcaaaagcc
    21421 ttattgctgg tcatatgacc gaaatcatgc agctgctgaa tctcgacctg gctgatgaca
    21481 gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt cgatgaaatt ttctccggtc
    21541 tggattacgc caatttcccg aaaatcaccc tcattgaaaa caaaatgaag gtcgatgaaa
    21601 tggtcaccgt gcgcgatatc actctgacca gtacctgtga acaccatttt gttaccatcg
    21661 atggcaaagc gacggtggct tatatcccga aagattcggt gatcggtctg tcaaaaatta
    21721 accgtatcgt gcagttcttt gcccagcgtc cgcaggtgca ggaacgtctg acgcagcaaa
    21781 ttcttattgc gctacaaacg ctgctgggca ccaataacgt ggctgtctcg atcgacgcgg
    21841 tgcattactg cgtgaaggcg cgtggcatcc gcgatgcaac cagtgccacg acaacgacct
    21901 ctcttggtgg attgttcaaa tccagtcaga atacgcgcca cgagtttctg cgcgctgtgc
    21961 gccatcacaa ctgattaaaa ggcaggaacc atggagcgca acgtcacgct cgattttgtt
    22021 cgcggcgtcg ccattctggg gatcctgcta ttaaacatca gcgcctttgg gctaccaaag
    22081 gcggcttatc tcaatcccgc ctggtacgat gctattacgc cgcaggatgc atggacctgg
    22141 gcatttctcg atctcatcgg ccaggtgaaa ttcctcacgc tttttgcgct gctgtttggt
    22201 gctggcctgc aaatgttgct gccccgtggc agacgctgga tccagtcgcg gttaacgctg
    22261 ttagtcttgt tgggctttat tcacggttta ttgttctggg acggcgatat tctgctggct
    22321 tacgggctgg tggggcttgg cgttttgctg ttattggggc tgatttccga cagccagacc
    22381 agccgcgcct ggacgccgga tgcatcggct attttgtatg aaaaatactg gaagcttcac
    22441 ggcggcgttg aagcgatcag taatcgtgcc gatggtgttg gcaacagttt actggcactg
    22501 ggcgcacagt atggctggca actggcaggg atgatgctca ttggtgcggc gctgatgcgc
    22561 agcggctggc tgaaagggca gttcagctta cgtcactatc gtcgtactgg ttttgtgctg
    22621 gtggcgattg gggtgaccat taaccttcct gccatcgccc tgcaatggca gctggactgg
    22681 gcgtatcgct ggtgtgcctt cttacttcaa atgccgcggg aactgagtgc gccgtttcag
    22741 gcgattggct atgcgtcgct gttttatggc ttctggccgc aattgagccg ctttaagctg
    22801 gtgcttgcga tcgcctgcgt cggacggatg gcgctgacca actatctatt gcaaacgctg
    22861 atttgtacca cgctttttta ccacctcggt ttgtttatgc attttgaccg cctggagctg
    22921 ctggcgtttg ttattccggt atggctggcg aatatcctct tctctgttat ctggctgcgt
    22981 tacttccgcc aggggccggt ggaatggctc tggcgtcagt taactttgcg tgctgccgga
    23041 ccggcaatat ctaaaacatc aagataacga tctggattac attcgttaac aaaacggctg
    23101 taaccgtttc cattgctgtg actcgattca cgaagtcctg tattcagtgc tgacaaaata
    23161 gccgccagca agcagtcatt tactgcaatc tcataacagg tagtgaatat gatcaccatt
    23221 cgtgatgtag cgcgtcaggc tggcgtctct gtggcaacgg tttcccgggt gctcaataac
    23281 agcacgctgg tcagtgccga cacgcgtgaa gcagtaatga aagccgtgag tgagctggat
    23341 tatcggccaa acgccaatgc ccaggcgctg gcaactcagg ttagcgacac cattggcgtg
    23401 gtggtgatgg acgtttctga tgcgtttttc ggcgcgctgg taaaagcggt ggatctggtc
    23461 gcccagcagc atcagaaata cgtgctaatc ggcaatagct atcatgaagc ggaaaaagag
    23521 cgtcacgcca ttgaggtgtt aattcgccag cgttgtaatg cgttgattgt tcactcaaaa
    23581 gcattgagtg acgatgaact ggcgcaattt atggataaca ttcccggtat ggtgttaatc
    23641 aaccgcgttg tgccggggta cgcccatcgt tgcgtttgcc tggataatct cagcggtgcc
    23701 cgaatggcga cgcgcatgtt gctgaataac ggtcatcaac gtattggtta tctttcttcc
    23761 agccacggca ttgaagatga cgccatgcgt aaagcaggct ggatgagtgc gttgaaagag
    23821 caggatatta ttccgccgga aagctggatt ggcactggta cgccggacat gccgggcggt
    23881 gaggcggcga tggttgaact gctggggcgc aatctacaac ttaccgctgt atttgcttat
    23941 aacgacaata tggccgctgg cgcactgaca gcattaaaag ataatggcat tgcgattccg
    24001 ttacatctct caatcatcgg tttcgatgat attcccatcg cccgttacac cgacccgcaa
    24061 ttaacgaccg tgcgttatcc cattgcttca atggctaaat tagccaccga actggccttg
    24121 cagggggcag caggcaatat tgatcctcgt gccagccact gttttatgcc gacgttagtg
    24181 cgtcgccatt ctgtcgcaac gcgccagaat gcggcggcga tcactaactc aacaaatcag
    24241 gcgatgtaac cgctttcaat ctgtgagtga tttcacagta tcttaacaat gtgatagcta
    24301 tgattgcacc gttttaatgt tgtaacccgt atgtaacagt gaataatcac tttcaccgag
    24361 gtaatagcgt cataacaaca attaaagccg ttttctggag cgttaccggg catggaagaa
    24421 cgaattttaa aaagtgagct tcggcgttca gtaacacttc attaactcta ctgccccgcc
    24481 gagcatttat ctcaagcact accctgcata agaaaaaccg gagataccat gaataagaag
    24541 gtgttaaccc tgtctgctgt gatggccagc atgttattcg gtgccgctgc acacgctgct
    24601 gatactcgca ttggtgtaac aatctataag tacgacgata actttatgtc tgtagtgcgc
    24661 aaggctattg agcaagatgc gaaagccgcg ccagatgttc agctgctgat gaatgattct
    24721 cagaatgacc agtccaagca gaacgatcag atcgacgtat tgctggcgaa aggggtgaag
    24781 gcactggcaa tcaacctggt tgacccggca gctgcgggta cggtgattga gaaagcgcgt
    24841 gggcaaaacg tgccggtggt tttcttcaac aaagaaccgt ctcgtaaggc gctggatagc
    24901 tacgacaaag cctactacgt tggcactgac tccaaagagt ccggcattat tcagggcgat
    24961 ttgattgcta aacactgggc ggcgaatcag ggttgggatc tgaacaaaga cggtcagatt
    25021 cagttcgtac tgctgaaagg tgaaccgggc catccggatg cagaagcacg taccacttac
    25081 gtgattaaag aattgaacga taaaggcatc aaaactgaac agttacagtt agataccgca
    25141 atgtgggaca ccgctcaggc gaaagataag atggacgcct ggctgtctgg cccgaacgcc
    25201 aacaaaatcg aagtggttat cgccaacaac gatgcgatgg caatgggcgc ggtagaagca
    25261 ctgaaagcac acaacaagtc cagcattccg gtgtttggcg tcgatgcgct gccagaagcg
    25321 ctggcgctgg tgaaatccgg tgcactggcg ggcacagtac tgaacgatgc taacaaccag
    25381 gcgaaagcga cctttgatct ggcgaaaaac ctggccgatg gcaaaggtgc ggctgatggc
    25441 accaactgga aaatcgacaa caaagtggtc cgcgtacctt atgttggcgt agataaagac
    25501 aacttggctg aattcagcaa gaaataagac tgattgggcg cagtcgttta ctgcgccctt
    25561 attgtatggc caacaaggta aaattatatg gtcagctcaa cgactccgtc ttccggggaa
    25621 tacttgttgg aaatgagcgg tatcaacaag tcctttcctg gtgttaaggc acttgataac
    25681 gttaatttaa aagtccggcc acattctatc catgcattaa tgggggaaaa cggtgcagga
    25741 aaatcgacat tattaaaatg cctgtttggt atttatcaaa aagactccgg caccatttta
    25801 ttccagggta aagagatcga tttccattcc gccaaagagg cactggaaaa tggtatttcg
    25861 atggtacacc aggagttaaa cctggtatta caacgttcgg tgatggataa catgtggctg
    25921 gggcgatatc ccaccaaagg catgtttgtc gatcaggaca aaatgtaccg cgaaaccaaa
    25981 gcgatttttg atgaactgga tattgatatc gatccgcgtg cgcgcgtcgg cacattatcc
    26041 gtttcgcaaa tgcagatgat cgaaatcgcc aaagcgtttt cctataacgc gaaaattgtg
    26101 attatggatg aaccgacttc ttcgttaacc gaaaaagagg tcaatcatct gttcactatt
    26161 attcgtaaat taaaagagcg cggctgcggt attgtttata tctcgcataa aatggaagaa
    26221 atcttccagt tatgtgatga agttaccgta ttgcgcgacg gtcagtggat cgccaccgaa
    26281 ccgctggcag gactgacgat ggacaagatc atcgccatga tggttgggcg ttctcttaat
    26341 cagcgttttc ctgacaaaga aaacaagccg ggcgaagtca tcctcgaggt gcgtaaccta
    26401 acgtcactgc gccagccgtc gattcgcgat gtctcgtttg atctgcataa aggggagatc
    26461 ctcggtattg ccgggctggt gggggcgaaa cgtaccgata ttgttgagac gttatttggt
    26521 attcgcgaga aatcggctgg caccattacg ttgcacggca aacagatcaa taaccataat
    26581 gccaacgaag ccataaacca cggatttgca ctggtaactg aggagcgccg ctcaacggga
    26641 atttatgcct atctggatat tggttttaac tcgttaattt ccaatattcg caactacaaa
    26701 aataaagttg gtttactgga taactcgcgg atgaaaagcg atacccagtg ggtgattgat
    26761 tcgatgcggg taaaaacgcc gggtcatcgg acgcaaattg gttcgctctc cggtggtaat
    26821 cagcaaaagg tgattattgg tcgctggcta ctaacgcaac cagaaatatt aatgctcgat
    26881 gaaccgacgc gcggtattga tgtcggggcg aagtttgaaa tttatcagtt aattgccgaa
    26941 ctggcgaaga aaggcaaggg gattattatt atctcctctg aaatgcctga gttgttaggg
    27001 ataacagacc gtattctggt catgagcaat ggtctcgttt ccggaattgt cgatacaaaa
    27061 acaacaacgc aaaacgaaat tctgcgtctt gcgtctttgc acctttaaga ttaggggctt
    27121 cccatgagtg cgttaaataa gaaaagtttt cttacttacc tgaaagaggg cggtatttac
    27181 gtcgttcttt tagttttgct ggcgattatt attttccagg acccaacatt tttaagtctg
    27241 ttgaacttaa gtaatattct cacccagtca tcggtgcgta ttattatcgc gctcggtgtg
    27301 gcagggttaa ttgtcaccca ggggaccgat ctttctgctg gtcgtcaggt agggctggcg
    27361 gcagtggtgg ctgcgacatt attgcagtcc atggataacg ccaacaaagt gttcccggaa
    27421 atggcgacga tgccgattgc gctggttatt ctgattgtct gtgccattgg tgcggtgatc
    27481 ggtttgatca acggtctgat tatcgcttat ctcaacgtga cgccgttcat taccacgctc
    27541 ggcacgatga tcatcgtcta tggcatcaac tcgctctatt acgactttgt cggggcgtcg
    27601 ccaatttctg gttttgacag tggcttctct acctttgctc agggctttgt cgcgctgggg
    27661 agtttccgtc tctcttacat caccttctac gcgttgattg cggtggcgtt cgtctgggtg
    27721 ttgtggaaca aaacccgctt cggtaagaac atttttgcca ttggcggtaa cccggaagcg
    27781 gcaaaaatat ctggtgtcaa cgtcggcctg aacctgctga tgatctacgc gttgtctggc
    27841 gtgttctatg cctttggcgg gatgttagaa gccggacgta tcggctctgc caccaacaac
    27901 ctcggcttta tgtatgagct ggatgctatc gcggcgtgcg tggtaggcgg tgtatcgttc
    27961 agcggcggtg tggggacggt gattggcgtg gtgaccgggg taattatttt taccgtcatc
    28021 aactatggcc tgacgtatat cggcgtaaac ccatactggc agtacatcat caaaggggcg
    28081 attattatct tcgccgtagc gctggattca ctgaaatacg cgcgtaagaa gtgatgtgat
    28141 tgcggatatt gccggatgcg tctctggttt tacaacgtta ccgggtgttc tttctcgcct
    28201 ttcttaaact tcacttcccc gagctcaata caacccaccg ggcagacgtg accacaaagc
    28261 agacaaccca cacatttctc ggtattacaa tgcggtgtgc gggttttctc gctccattcc
    28321 atcgcctggt gaccgccgtc gtaacaggaa atataacagc gtccacagcc aacacatttg
    28381 tcaagattga tacggggata gacaatataa ctgcggtcta aatcttccgc cgggacaata
    28441 ttgttattcg ccagacctac catctcctgc aacgaatcaa atccctgatc ggcgagataa
    28501 tgcgataacc cgctcgccat atcttctacg atgcgatagc cgtactgcat aatgccggtg
    28561 gtgacctgta acgttgctgc gccgagcaat aaaaactccg ccgcatcttc ccaggtttca
    28621 atgccgccga taccgctgat tgggaaatcg cgcagttctg gatgggtgcg catttgctga
    28681 atgaagcgca gggcgatcgg ttttaccgct ttaccggaat atccggaaat actcgattta
    28741 ccgttaacga tcggcatacc gattttctga ttaagatcga tattggtgat ggatttaacg
    28801 gtgttaattg ccgcaatgcc atctgcgccg ccgcgcttcg ccgccagcgc cacttcgcac
    28861 atatcaccga tattcggcgt catcttcgcc agcattggca gcgtggaacc ccgtttcact
    28921 gcccgacaat atttttctac cagctccggg ctttgcccga catcgctacc catcgcatga
    28981 gaagtcattt gcggacagga gaagttacac tcgatcatat ccgcgccagc tgctcccatt
    29041 gctgctcatt ttcccccatg atcgaagcga tcaatacttt gtccgggtaa tcttccttca
    29101 gccgacgcag ggcggtcaga ttctcttcca acggatgttc agcaatctgc tccatatttt
    29161 tgaagccgat aaaaccggta tcttctttca ccagatgatc aaaacgcggc gagacttcgt
    29221 tggcgataaa aaagccgatc gttttaaaca ccacaccgcc ccaacctgtg tcgtaggctt
    29281 tggcacacat ctcatagcag ttgcctaccg gcgaagaaga gaggcagaac gggttgggaa
    29341 agttcacgcc gcaaaaagta atcgaaagat ctttcgttaa catgagcaag ctccctctaa
    29401 atagtgatga atcgcctcgg cggcttcttt cccggttttc acggcataga ccacggtttt
    29461 gtcaccctca acaatatcgc cagcagcaaa gacttgcgga tcgcggatct gataattttg
    29521 tgttttgatg gtgttacgct gctgctctaa ctccgcaaag gcatccagtc tggcatgctg
    29581 accgacggcg agaataattt tatctgccgc catcgtcagt tcgcctgata accgtacatg
    29641 cttaaacgtg actttattgc cttccacggc tactggcgtg aatccatcga tgatcgaaac
    29701 gcccagctcc cgggcgctgg caaactcttt ttcgcttgcc ggaaactcat ctaactcttc
    29761 acgtgctacg caagttaccg cctgacagcc gagaactttc agcgtgctgg ctacgtccat
    29821 cgcgacatca ccgccgccga taattaatgc gctttgtgga atgctgatat cgccttgcgc
    29881 ctgtcgtaca cgttgcagga agtcgacggc aatctcaacg tcgctatgct caaacagcgg
    29941 tagcccggaa ccgcttgata accccacggt gaccagtacc gcgcggtttt ccgctttcag
    30001 ttgctccagg gtgagtgttt taccgatttc gttattgcac ttaatggtca cgcccatttt
    30061 ttcaatacgg gcgatctctg catccagcac tgattgcggt aaccggaatt gcggaatacc
    30121 gttacgcaac cagccaccgg ggtgcgcttc tttctcataa atcgtgacgt cataaccctg
    30181 gtttgtcagt gtcacactgg cctgcaatcc ggcaggacct gcgccaataa tcgcgacttt
    30241 gccgagcgtt ttagtaccgg gctgataaat ttccatcccg gtttgttgtt caaaatcagt
    30301 aacaaaacgc tgtaagcggc cgatatcaat gggagcatca acaccggcac gggtacaacc
    30361 gctttggcat aatttctccg tcgggcacac tctggcacaa acggcaccga gggcattatt
    30421 ttcgcgaatt gtctcggcag cgcctttaaa attacgaaag tagattgagc gaataaattt
    30481 ccccggatcg gtctgtgccg ggcaagcctg actacaggga gcgtcgtgac ataataaaca
    30541 gcgagaggct tctttaatcg ccagtaacgg cgtaaaagcc ggagtgagtt catccagata
    30601 attttgttgc ggcatgtgat cttccttaat gctatgagta attatggcga acgctaaata
    30661 aatattgcgt gctgatatta aggctatgcg cgtggggaaa tttaagctgt gaaaggattc
    30721 acaaatagct gcggttcaga atgttatatc cgcagcttat tgagtgaagt aataattatt
    30781 ctcaataatt aacagaaaag agaaaatatt aacgatagaa aataagataa acaatcatgc
    30841 cgatagataa aattccgaag ccaatatatg ccaccatata agcgaagaag agtccgggat
    30901 tttttgctcg ccagacatac accggttccg gtgcattttt aacccgttta tcaatcgcgc
    30961 ctgcgcgcca gcctttccat gcttcgcgga aacaaaatat cgggataagg aaaaaaacgg
    31021 caacgacaaa aaactgctgt acatccataa ctactttcct tgtttctttt gtaattcaag
    31081 taactgttcg ggtgtaacgg cgggataccc ctgcgcatct tccggcacct ggtgcatagc
    31141 cggaataggg accagcggcc ctaaaaaacg cggttcacgt ttaaaaatat aaaggtcagc
    31201 cagcgcaccg aaacgggcgg caaattcgcg aatacgtact gacagcatat ctttcggtga
    31261 cggtacggca taacactgag cctgaatccc catatgcagc gcaataaata atgctcgctc
    31321 acagtggaaa cgttgggtga taataatgaa atcattagta tcgaaaactt tgcgtgtacg
    31381 cacgatggaa tccagcgtac gaaagcctgc gtaatcgaga acaatatctg atgggtcgac
    31441 accagcagcg attaaatctt tgcgcatggt catcggctcg ttataacttt gcaatgcgtt
    31501 atcgccgctc agtaatagat aatttacctt accgctgtta taggcattaa tcgctccttg
    31561 aatgcggtag cgataatact gattaataac gccggtacga taatattttg ctgtgccgag
    31621 caccacgccg acctggcggt aggggagatc ctgcaattcg tcgtagatat aaggcgcggt
    31681 tttccagctc atccagcgat cgaggccgag cacagtcaac agcagcaagc cgatcaggac
    31741 taacaggctg aggaacacgc gctttaacat gcaggagact cgagctaagg aaaatttcca
    31801 tcaggctact ttacccgctg gttttgcgca agaaacggca aactgtttgc cgggaatttc
    31861 gacaccagga gagggctggc aagccggaca aggcgttcac gccgcatccg gcaccaggct
    31921 taagcgagaa gcacgcggtc gatactgtga cagccgagag ctttcagcgt tgcggaggtg
    31981 gcatcccact gaatcaacgg cgcatcggct ttttctgcca gaaccgcgcg ctggatatcc
    32041 gggtaatcat aacccttgag attcaacaga attaacgccc cctgcaacgg cggcagagtc
    32101 gggttgaatg cggcgttttc agcgtagctg ccgctgaaaa tacgaccgtc tttacattcc
    32161 agcgcgacac cgcttggtga cttactgtaa ggcatgtgcg aacggtttgc cgctgcaatc
    32221 gccgcctgag aaagcgcatc accagtcagc gcatagccgt gatcctgctc gtccatcagc
    32281 agcgttttaa tctccagatc tttcggccca aaggcatccg gcagatagtc acgcagcgcg
    32341 tgtgcctcgc ggcccggcaa atgaatacgc agatccagac cgctgttcag ttcattcata
    32401 aactgacggc agtgaccaca aggcgtatag ttaacggtga tggctgcaag cgctttttca
    32461 ccactcaacc aagcgtggct gatcgcgctt tgttcggcat gaacggtttg ctgcattgtt
    32521 gcaccaataa attccatatt ggcaccaaaa taccaggttc cgctcacacc gcgcgcaatt
    32581 gcgccaacat taaaattcga caatggcgta cgcgcacagg ccgccgccag cggaagtagt
    32641 gcgaatgcca gcgcgtcttc gtccagtccc gttgcgctct tcagcgatga gacttgctcc
    32701 ccggtcaaca aagcggggaa gtacttgtct gccagaatag gttccagtgc agattgcaag
    32761 ttatccgcaa gttgggcaaa agcggtttga aaacgtggat gcatggcgtt gcctcataat
    32821 catgtaatgg gataacagtt tacgaacccg ttatggcttt atatgtgatc tgaatctcat
    32881 taattatgca acacgtgaaa cttacatcaa aaatgcgcga cgtatcgcaa attttagccc
    32941 attactgcca gaataatcgg gaaaaggaac ggtgcgatca gcgaagtaat tatcccgcat
    33001 aacaccagcg ctagcgaact aaatgcacct tcctgataat ccagctcggc gcagcgcgcc
    33061 gtaccgaggg cgtgcgaggc agtccccatt gccagaccgc gcgcagcttt ggtacgaata
    33121 cgcatcgcat taagcaatgt atggccaaat accgcgccga ggatgccgac gaaaatcacg
    33181 caaactgcgc tgattgccgg aataccgcca atactgccgc caaccgccat tgcaataggc
    33241 gtggtgactg atttcggcag gattgacgcg gcgatttccg gtgaagcgcc catcaacaat
    33301 gccacggaag tccccgtcac catcgcaacc acgctgccga taaaacagat ggtgatgatc
    33361 gatttccagc gcgcgcggat ctggtgtagc tgctcatata aaggataggc cagcgcgacg
    33421 accgccggtt gcagcagatc gtttagcact tcgctacctt taaagtaact gtcgtaagag
    33481 atgccggtca gcattaaaaa aggaatgatc accaccattg ctaccagcaa cgggttaagc
    33541 aggggaaact tataccgtgc cgccagtttg cgggcggcaa aaaagacaat caacgtcagc
    33601 ggtaatgacc accagatatt cgccatcatt cttctgatcc tttctgacct acgactttac
    33661 gttcaccgtg taccagttgt gaactccagc tcaccaccag aaaaaccacc agcgtactga
    33721 cagcacagga aaccactacc gggccaaact gcgcgcggag caaatcaaaa tattgcatga
    33781 cgcctacgcc aatcggcaca aacaatagcg ccatatagcg aatcagtacg tagcaccccg
    33841 gattgaccca tttcgccgga agaatttgca aggccagcag gacaaacagg atcagcatcc
    33901 cgatgatgct gcccggaatg gttaccggta gcagggaagc aatgaaaatg cctgcataca
    33961 ggcaggcata aatcaggacg aaagcgcgta aatattgcca gataatgttc agtgtcttac
    34021 tcataatcaa caccccagat gagatggatt catcatacaa ttaattcgta aaacgtgcta
    34081 ccgatcacat taagaaatca gcgcttattt tttcgctacc cggtattacg caatgagcag
    34141 cgaactcagg catacatcaa gcatagagag ggattgcagt ttgcagcaat ccctctttgc
    34201 agacctttaa tccgctgacc ataggcgcta acatagcgcc tcattttttt gcgggtgatg
    34261 atatgcgtgt gttactggca ccgatggagg gagtgcttga ctctctggtg cgtgaattgc
    34321 tgaccgaagt taacgactac gatctgtgca tcaccgagtt tgtccgcgtg gtggatcaac
    34381 tgctgccggt aaaagtcttt catcgcattt gccctgagct acaaaacgcc agccggacac
    34441 catctggtac gctggtgcgc gtgcagttgt taggtcagtt cccacaatgg ctggcagaga
    34501 acgccgcccg tgcggtggag ttaggtttct ggggcgtgga tctcaattgc ggctgcccgt
    34561 cgaaaacggt taacggtagc ggcggcgggg cgacgttact caaagatcct gaactcatct
    34621 accagggggc aaaagcgatg cgtgaagctg taccggcgca tttgcctgtc agcgtgaaag
    34681 tgcgtctggg ctgggacagc ggtgagaaga aatttgaaat cgccgatgcg gttcaacagg
    34741 ctggcgctac ggagctggtg gtgcatgggc ggacgaaaga gcagggttac cgcgcggagc
    34801 atattgactg gcaggcgatt ggcgagattc gccagcggct gaatattccg gtgattgcca
    34861 acggtgaaat ctgggactgg cagagcgcgc aacaatgcat ggcgatcagc ggctgcgacg
    34921 cagtgatgat tggtcgcggg gcgctcaata ttcccaacct gagccgggtg gtaaaatata
    34981 acgaaccgcg aatgccgtgg ccggaggtgg ttgctttgct gcaaaaatat acccgtctgg
    35041 aaaagcaggg cgataccggg ttatatcacg ttgcgcggat taaacagtgg ttgagttatt
    35101 tgcgtaaaga atacgatgaa gcaacggaat tatttcagca tgttcgggtg ttgaataact
    35161 cccctgatat tgcaagggct attcaggcaa ttgatatcga gaaactctaa aaaccatagt
    35221 gatgtttttg acaatgtgtt agctctttat caataggccg cacggttgtt tggtggcgtc
    35281 aaaatcgccg aggcgttccc tgaaggccgg ggcagcccac atggatgtgg gctgagggcg
    35341 cgttttacag ggatgttacc tcgcgcccga cccggtagcc gtaagggata agccgagggc
    35401 accgcgcagc ggcgattttg ttcgccagag cccggggggg cagggggcgg cggcgattgg
    35461 tcgccccctg cgcgctcctt gcgccagtga ttatatgaag caatgcacat gacaggagcg
    35521 caacaggatg agagactgaa atattctcgt tattttcgga tctgatgaca ttaattaatt
    35581 ttaaaatatc atcttaaata cgccagtcac caccagtagc ccaatcagga aaataatcaa
    35641 tacagcccag agtataattt tcattggcgt tcctttattt gtgagtgctg aataagtata
    35701 ggaaaaaacg aagccctttg ccgttatcca taaacgatta agataaatcg gaagatatca
    35761 tttacttagt ctatattttt ctgggtatgt cgttttattg ctactgatgt attgccacgc
    35821 gaaactaaaa aatatatcgc aacacacggt aatctccgcg caccgtttct tacctgatta
    35881 ttcccccatg aatcgtgatt ccttttatcc agccatcgcc tgttttccgc tgttactgat
    35941 gctggccggg tgtgcgccta tgcatgaaac ccgccaggcg ttaagccagc aaacgcccgc
    36001 tgcacaagtt gacaccgcat tacccacggc gctgaaaaat ggttggccag acagccaatg
    36061 gtggctggag tatcacgata atcagctcac ttcattaatt aacaatgcac tacagaacgc
    36121 accggatatg caggtggctg aacagcgcat ttagctagct gaagcccagg cgaaggcagt
    36181 tgccactcag gatggtccgc agatcgactt ttcggcggat atggagcggc aaaaaatgtc
    36241 ggcagaaggg ctaatggggc cgtttgctct gaacgatccg gccgcaggta cgaccggtcc
    36301 gtggtacacc aacggaactt ttggcttaac ggcgggctgg catctcgata tctggggaaa
    36361 gaatcgggcg gaggttattg cccgcctggg tacggttaaa gcacgggcgg cggaacgcga
    36421 gcaaacccgc caattgctgg ctggcagcgt agcccgcctg tactgggagt ggcaaaccca
    36481 ggcggcgtta aacacggtct tgcagcaaat agaaaaagag cagaacacca ttatcgcgac
    36541 cgatcgccag ctatatcaga acgggattac ttcttcagtt gacggtgtgg aaaccgatat
    36601 taatgccagc aaaacccggc agcagctcaa cgatgtcgcg gggaaaatga aaattattga
    36661 ggcacggtta aatgcactca caaatcacca gactaagtca ttgaagctta aaccggtcgc
    36721 gttgccgaaa gtggcaagcc agcttcctga tgaactgggg tactccttac tggcccggcg
    36781 ggcagatttg caggcggcgc actggtacgt tgagtcatcg ttaagcacta ttgatgcggc
    36841 aaaagcggca ttttatcctg acatcaacct gatggccttc ctgcaacagg atgcgttgca
    36901 cttaagcgat ctgttccgtc attccgcgca gcaaatgggc gttacggcag gcctgacgct
    36961 acctattttc gatagtggtc gtcttaacgc caatctcgat atcgcaaaag ccgaaagcaa
    37021 cttgtctatc gccagctaca acaaagcggt ggttgaagca gtgaatgacg tggcgcgggc
    37081 agccagtcag gttcagacac tggcggagaa aaaccagcat caggcgcaaa ttgagcgcga
    37141 tgccttgcgt gtggtaggtc ttgcgcaggc ccgctttaac gcgggcatca ttgctggttc
    37201 ccgcgtcagc gaagccagaa tcccggcgct gcgtgagcgg gccaatggcc tgttattgca
    37261 agggcagtgg ctggatgcct ccattcgact caccggtgcg ctgggcgggg gttacaaacg
    37321 ctgatgatat gcgccttcta tacttaacgt ttattcagcg ttaagtggag aactcgatgg
    37381 cataggtcgc gataattacc gcttccgatt cggggatcgg caaagagtgc gcgttattac
    37441 tggcgcagca ggggtttgat attggcatta cctggcactc agatgaagaa ggggcaaaag
    37501 ataccgcgcg tgaggtggtt acccacggcg tacgtgcgga gatcgtgcaa ctggatctcg
    37561 gcaatctgcc agaaggtgcg caggcgctgg agacactcat ccagcgattt gggcgtattg
    37621 atgtgctggt gaataatgcg ggtgcaatga ccaaagcgcc gtttcttgat atggcttttg
    37681 atgagtggcg caagattttt accgttgatg ttgatggtgc atttttatgc tcgcaaattg
    37741 cggctcgtca gatggtgaaa taagggcagg gcggtcgcat catcaacatt acgtcggtac
    37801 atgaacatac gccgctgccg gatgccagcg cctacacagc cgctaaacat gcgctcggcg
    37861 ggttaaccaa aacgatggcg ctggagttgg tcaggcataa gattttggtg aacgcagtcg
    37921 cgcctggggc gatagccacg ccaatgaaca gcatggatga tagcgacgtg aagcccgacg
    37981 cggagccttc gattcctcta cggcgttttg gcgcaacgca tgagattgcc agcctggtgg
    38041 tgtggctttg ttcggagggc gcaaattaca cgacagggca gtcgttgata gtggatggcg
    38101 gctttatgtt ggcgaatccg cagttcaacc cagaatagcg cagtgaggtg cgtggatatt
    38161 tcaatgggtt aggccagata agacaccagc gtcgcatcag gcatggaaca cccggtgccg
    38221 gatgtggagt gaatgcttat ctgtggcaac tgctttacgc ctgattatcc ggctttttct
    38281 tcccgcgccg tttcagccac cagcgcacgc ccaccaccag aaccacgacc agaatcaacc
    38341 agacccagtg cttcaaatgc tggtcgagat tgtgcaacca cggcgcaatc acctgaccac
    38401 cagcgtaacc aatagtggta aaaatcaacg cccaggcaaa tgcgccgaga atattcagcg
    38461 gcagaaagat tttcggcggc agctggctgg caccaatcag cgtcgggcca atcacccgaa
    38521 agccatacat aaagcgcgta ccaatgacaa acagatacgg atggcgttgg ataagtttct
    38581 gtgcccgctc aattttatcc tgatgtttcg agaaacggcg taacagcttg ccgccaaacc
    38641 gccgcccgca cagatagagc acctggtcgc caatcatgcc accaagcgcc acagaaagta
    38701 ccaccagtgg gaactttaat agcccctgat gtgccgcaac gcctcccagc aaagtcacgg
    38761 tttcaccttc cgccaggcta ccgataacca gcgcggcata accatattgt gagatgagtg
    38821 tattgagatc cataggccag tttgtctccc tttggttgcc tgtaagcata caccctgtga
    38881 atcattcacg ctgaaaaagg acatacatca gacggtgcaa attacatgga tgaacaaaaa
    38941 ataatcacaa gctggcttat acttgaactg tcaggttcat gaactcacaa caagggggcg
    39001 gtatgagcca tgtctgggga ctgttttccc atcccgatcg tgaaatgcag gtgattaatc
    39061 gcgaaaacga aacgatttct caccattaca cccaccacgt tttgctgatg gcggcgattc
    39121 cggtgatttg cgccttcatt ggcactacac agattggctg gaattttggc gatggcacta
    39181 tcctgaagtt atcctggttt accggactgg cgctggctgt cttgttttac ggcgttatgc
    39241 tggctggggt tgcggtgatg gggcgggtca tctggtggat ggcgcgcaat tacccgcagc
    39301 gtccgtctct tgcgcactgc atggtcttcg cgggctacgt tgcaactccg ctgtttttaa
    39361 gcggtctggt ggcgctttat ccactggtct ggttgtgtgc gctggtcggc acggtggcgc
    39421 tgttttacac cggctacctg ctttaccttg gtattccgag cttcctgaat atcaacaaag
    39481 aggaaggcct tagcttctcc agttcgacgc tggcgattgg cgtgctggta ctggaagtgc
    39541 tgctcaccct aaccgttata ctatggggct acggttatcg tttgttctag aaagttcttt
    39601 gacgttgcat tgctggcgta aatgtcagca atgcagcatt tactcacgat tctcctttga
    39661 cgatcgcact ttttgctcgt tatgatggcg tcgctagcct cagtaaatcc ttactccggc
    39721 ggtgcgcaac ccgtgcgcgt gaaccactat ctgaatgctc atcatgccga aatttcgagt
    39781 ttctttattt agcttggccc tgatgctggc tgtgcctttt gcaccgcagg ccgttgcgaa
    39841 aacggcagcc gctaccaccg cttcacaacc ggaaattgcc tccggtagcg cgatgattgt
    39901 tgatctgaat accaacaaag tgatctattc gaaccacccg gatctggtgc gtccgattgc
    39961 gtctatcagc aaattaatga ccgcgatggt tgtgctggat gcacgactgc cgctggatga
    40021 aaaactaaaa gtggatatca gccagacgcc ggagatgaaa ggggtctatt cgcgcgtacg
    40081 actgaatagc gaaatcagcc gtaaagatat gctgttgctg gcgctgatgt cttcagaaaa
    40141 ccgcgcggcg gcaagccttg cgcaccatta tcccggtggt tacaaagcct ttattaaggc
    40201 aatgaatgcg aaagcgaaat cgctcggaat gaacaacacg cgctttgttg aacctaccgg
    40261 attgtcggtg cataacgttt caactgcccg tgacttaacc aaactgctca ttgccagcaa
    40321 acaatatccg ttgatcgggc agttaagtac tacccgggaa gatatggcaa ccttctctaa
    40381 cccgacgtat acgctgccgt tccgcaatac taatcatctg gtgtatcgcg ataactggaa
    40441 tattcagtta accaaaaccg gctttaccaa tgcggcgggc cattgtctgg tgatgcgtac
    40501 ggttatcaat aataaaccgg tggcgctggt agtgatggac gcgtttggca aatataccca
    40561 ttttgccgat gccagccgcc tgcgtacctg gattgaaacc ggtaaagtga tgcctgtgcc
    40621 ggcagcagcg ttgagctata aaaaacaaaa agccgcccaa atggcggcgg cggggcagac
    40681 ggcacagaac gattaagtga attcggatgg cgatactctg ccatccgtaa tttttactcc
    40741 acttcctgcc agtttttccg tttactggtt ttaccgatcc ccggattcat gctattggtc
    40801 ggatcattct cgcgatagaa cttctgcaac gtctccggtg ctttatacaa atgaccgacg
    40861 ttatgctcgg cagggtactg cgcgccgcgc tgctgtagca gttccagcat ctgttctttt
    40921 aacgcatgca catccacgcc ttttttcact atgtaatcct gatggaagac atagcacata
    40981 aaatggccgt aatagagctt gtgcaccagc tggctgtcga tctccggcgg taaatgctca
    41041 taccactcgg tgtcgttacg ccgcagagcg atatccagtg ccagaatgtc ttcgacttca
    41101 tcggaatgca ccgcctgata acgaattgct gcgcccgcag cggcgaaacg gtgtaaaaac
    41161 gctttgctac cttcctccgg cgtacagaca aagaaatcgc cctcggcctg tttgaaataa
    41221 tccaccagcc acgatttggc ttcaccgacg ccatcgcctg ccatttttaa cagcagatga
    41281 tgctcgtatt tatcgcgcca gtttttcatg cgcggcggta aatggctggg gaacaggtga
    41341 ccgaattttt gcattgcacg gtcagtaaaa tgcggacgga agaatttcac tttctccagc
    41401 atcgcatcgg tgcgtccctt gagattaaag aagaacggca tcttgtcggt gcctaactta
    41461 tcaatcatca ggaaggtgtc tttgccgtat ttttccgcaa tatcgtagat atcccggtgc
    41521 atatattccc cggcaaccgg cagattttcg aagttagcca gaatatgacg gcggatttcg
    41581 gtcagcactt ccggctggtt ggtgccgata taaaacacct gctgattttt ttccgcttcg
    41641 aaggtatcaa gacgtaccgc aaagacggcc agcttcccgg cgcaaccaga agattcaaat
    41701 aaccgatccg gatcggcgtt ataacgtgcg ggcgtgtcgg cttcaatatc acgaacgcgg
    41761 tggacatagt cataatcgtg ggcgtgacga ccatcgtgac gcacatcgtc atctttgatg
    41821 cgatcgtcat ccagcttgct aaggatctgc tccggcgttt cgcccagatc aatccccaga
    41881 tggttcacca gcgtcagttt gccatcttca tctatacgcg cgaataacga catttcggta
    41941 tacgccgggc cacgttgcac cagcgagccg ccggagttgt tacaaatacc gccgatgacc
    42001 gatgcgccta tacacgatga tccaatcacc gaatgcggtt cgcgtcccag cggtttgagg
    42061 gctttttcca gcgaatagag cgtggtgccc ggataggcca gcacctgttc gcccttgccg
    42121 agaacgtgca gcttgtcgag acgcagggtg ctgatgatca cgatatcgcg atcatagtcg
    42181 ttaccgtttg gcgtcgatcc ttcggtcagg cctgtattgg cagcctgcat cagaataatt
    42241 ttgtcggcgg tgacgcaggc tttcagcacc cgccacaatt ccagtagtga gccagggaaa
    42301 acgacagcca gcgcgtcgcc ctgaccagaa cggaagccct tgcgatagcg ggccgttttt
    42361 gcgggatcgg tgagcaggtg tgaatgaccg accagacggg caagttcatt caaaaaggct
    42421 ttattatcag ttgttgtcat ggaagacatt ttccactcct tgtggtggcg aaaaaatatc
    42481 acaaactaga atagcgcctt gtatgacaat tcagcgagtg aatattttga aaattcagac
    42541 aataaactga gcattctttt tctctctcca tcatgcttat ggcaaactac ggtttttgca
    42601 ccattttcgc tgccacgtcg ggcaacaaag gaagaaaaat ccatatgaaa tggctatgtt
    42661 cagtaggaat cgcggtgagt ctggtcctgc agccagcact ggcggatgat ttattcggta
    42721 accatccatt aacgcctgaa gcgcgggatg cgttcgtcac cgatctgctc aagaaaatga
    42781 cggttgatga gaaaattggt cagctgcgct taatcagcgt aggcccggat aatccgaaag
    42841 aggcgatccg cgagatgatc aaagacggtc aggttggggc gattttcaac accgtaaccc
    42901 gtcaggatat ccgcgccatg caggatcagg tgatggaatt aagccgcctg aaaattcctc
    42961 ttttctttgc ttacgacgtg ctgcacggtc agcgcacggt gttcccgatt agcctcggtc
    43021 tggcctcgtc ttttaacctc gatgcggtga aaacggtcgg gcgtgtctct gcttatgaag
    43081 cggcagatga tggcctgaat atgacctggg caccgatggt cgatgtctcg cgcgatccgc
    43141 gctggggacg tgcttccgaa ggttttggcg aagatacgta tctcacctca ataatgggta
    43201 aaaccatggt ggaagcgatg cagggtaaaa gcccggcaga tcgttactcg gtgatgacca
    43261 gcgtcaaaca ctttgccgca tacggcgcgg tagaaggcgg taaagagtac aacaccgtcg
    43321 atatgagtcc gcagcgcctg tttaatgatt atatgccgcc gtacaaagcg gggctggacg
    43381 caggcagcgg cgcggtgatg gtggcgctga actcgctgaa cggcacgcca gccacctccg
    43441 attcctggct gctgaaagat gttctgcgcg accagtgggg ctttaaaggc atcaccgttt
    43501 ccgatcacgg tgcaatcaaa gagctgatta aacatggcac ggcggcagac ccggaagatg
    43561 cggtgcgcgt ggcgctgaaa tccggaatca acatgagtat gagcgacgag tactactcga
    43621 agtatctgcc tgggttgatt aaatccggca aagtgaccat ggcagagctg gacgatgctg
    43681 cccgccatgt actgaacgtt aaatatgata tggggttgtt taacgaccca tacagccatt
    43741 tggggccgaa agagtctgac ccggtggata ccaatgccga aagccgcctg caccgtaaag
    43801 aagcgcgtga agtggcgcgc gaaagcctgg tgttgctgaa aaaccgtctc gaaacgttac
    43861 cgctgaaaaa atcggccacc attgcggtgg ttgggccact ggcggacagt aaacgtgacg
    43921 tgatgggcag ctggtccgca gccggtgttg ccgatcaatc cgtgaccgta ctgaccggga
    43981 ttaaaaattc cgtcggtgaa aacggtaaag tgctgtatgc caaaggggcg aacgttacca
    44041 gtgacaaagg cattatcgat ttcctgaatc agtatgaaga agcggtcaaa gtcgatccgc
    44101 gttcgccgca agagatgatt gatgaagcgg tgcagacggc gaaacaatct gatgtggtgg
    44161 tggctgtagt cggtgaagca caggggatgg cgcacgaagc ctccagccgg accgatatca
    44221 ctattccgca aggccaacgt gacttgattg cggcgctgaa agccaccggt aaaccgctgg
    44281 tgctggtgct gatgaacggg cgtccgctgg cgctggtgaa agaagatcag caggctgatg
    44341 cgattctgga aacctggttt gcggggactg aaggcggtaa tgcaattgcc gatgtattgt
    44401 ttggcgatta caacccgtcc ggcaagctgc caatttcctt cccgcgttct gtcgggcaga
    44461 tcccggtgta ctacagccat ctgaacaccg gtcgtccgta taatgccgac aagccgaaca
    44521 aatatacttc gcgttatttt gatgaagcta acggagcgct ttatccgttc ggctatggtc
    44581 tgagctatac cactttcacc gtctctgatg tgaaactttc tgcgccgacc atgaagcgtg
    44641 acggcaaagt gacggccagc gtgcaggtga cgaacaccgg taagcgcgag ggggccacgg
    44701 tagtgcagat gtacttgcag gatgtgacgg cttccatgag tcgccctgtg aaacagctga
    44761 aaggctttga gaaaatcacc ctgaaaccgg gcgaaactca gactgtcagc ttcccgatcg
    44821 atattgaggc gctgaagttc tggaatcaac agatgaaata tgacgccgag cctggcaagt
    44881 tcaatgtatt tatcggcact gattccgcac gcgttaagaa aggcgagttt gagttgctgt
    44941 aatgctttag taagggcgcg acgtttgcgc cctttgcctg atgttcatat cttaacttgc
    45001 tgaattttcg ccctttgtag gccggataag gcgctcgcgc cgcatccggc aggaaacgcc
    45061 ggaccgaata tgcctcttaa gtatttatct gattctgcaa ctgaatcctt ccgctcaggc
    45121 taactctgtc attatcaact atgcttttct cttaattcgc tgaaaacaac agtaaaagaa
    45181 tgaggaaagc atcatgccac tcttaaagct ctgggcaggt tcactggtta tgttggcagc
    45241 cgtgagcctg ccgctacagg cggcttcccc cgttaaagtc ggttcaaaaa tcgataccga
    45301 aggcgcgctg ctcggcaaca tcatcttgca ggtactcgaa agccacggcg taccgacggt
    45361 gaataaagtg caactcggaa cgactcctgt ggggcgcggg gcgattactt ccgatgaact
    45421 ggatatctat ccggaatata ccggcaatgg cgctttcttc tttaaagatg aaaacgatgc
    45481 agcgtggaaa aatgccgggc agggctacga gaaagtcaaa aagctcgatg cggagcacaa
    45541 caagttaatc tggctgacgc ccgcgcctgc aaataacacc tggactatcg ccgtgcgtca
    45601 ggatgtggca gagaaaaaca aactcacttc gcttgccgac ctgagtcgtt atctgcaaga
    45661 gggcggcacc ttcaaactgg cagcctcggc agagtttatc gaacgcgccg atgcgttacc
    45721 cacgtttgaa aaagcctatg gctttaagct cggtcaggat cagttgctgt cactggctgg
    45781 tggcgacacg gcggtgacga ttaaagccgc tgcccagcaa acctctggcg ttaatgccgc
    45841 aatggcttac ggcactgacg gtccggtcgc ggcgctgggg ctgcaaacct taagcgatcc
    45901 gcaaggcgtg caacctatct acgcgcctgc accagtggtg cgtgaatcgg tgttgaagga
    45961 gtatccgcaa atggcacagt ggctacagcc agtcttcgcc agcctcgatg caaaaacatt
    46021 gcagcaactg aatgccagca ttgcagtgga aggactggat gccaaaaaag tggctgccga
    46081 ctacctgaaa caaaaagggt gggcgaagta atttcccgtg acttatctac gtattaatcc
    46141 tgttctggcg ctgctgttgt tgctgacggc aatcgcagcg gcgctgccgt ttatcagtta
    46201 cgcgcctaat cgtttagtct cgggtgaagg gcttcatctc tggcaactgt ggccgcaaac
    46261 gatctggatg ctggtgggcg ttggttgcgc ctggctgaca gcctgttttg tccccgctaa
    46321 aaaaggcagc atttttgcac tcattctggc gcaattcgtc ttcgtattgt tggtgtgggg
    46381 agctggaaag gcggcgaccc aactggcgca aaatggcagt gcgctggcgc gtaccagcct
    46441 cggcagaggt ttctggctgg ctgctgcgct ggcattgctg gcctgtagcg atgccatccg
    46501 ccgaatctcc acgcatccgc tgtggcgctg gttgttgcat atgcagattg ccattattcc
    46561 gctgtggttg ctgtactccg gcacgcttaa cgatctctca ctaatgaaag aatacgccaa
    46621 ccgtcaggat gtgtttgacg acgcgctggc acaacatctg acgttgctgt ttggtgcggt
    46681 gctgcctgcg ttagtgattg gtgtgccgtt gggcatctgg tgctactttt ccactgcgcg
    46741 gcagggggca attttttctc tgctcaatgt cattcagacc gtgccttcgg tggcgctctt
    46801 tggcctgttg attgcgccgc ttgccgcgct ggttacggcc tttccgtggc tggggaagct
    46861 cggcatagca ggaaccggaa tgacacccgc actgattgcg ctggtgctct atgccttgct
    46921 gccgctggtg cgaggcgtgg tagtcggttt gaaccagatc ccgcgcgatg tgctggagag
    46981 cgccagagcg atggggatga gcggggcgca gcgtttcctg catgttcagt taccgctggc
    47041 gttaccggta tttctgcgca gcctgcgggt ggtgatggtg caaactgtag gtatggcggt
    47101 gattgcggcg ttaatcggcg caggcggttt tggtgcgctg gttttccagg ggctgctaag
    47161 cagcgccatt gatttagtgt tgctgggggt gatcccggta attgttctgg cggtgcttac
    47221 cgacgcgctg ttcgatttgc ttatcgcact gctgaaggtg aaacgtaatg attgaattta
    47281 gccatgtcag caaactgttc ggcgcacaaa aagccgttaa cgatctcaat ctcaattttc
    47341 aggaagggag tttttcggtg ctgattggca catctggctc cggcaaatcc accaccctga
    47401 aaatgattaa ccgcctggtg gagcatgaca gcggagtgat ccgctttgcc ggagaagaaa
    47461 ttcgctcgct gccagtactg gagttgcgcc gccggatggg ctatgccatt caatctattg
    47521 gcctgttccc ccactggagc gtggcgcaaa acatcgctac cgtgccgcaa ttacaaaaat
    47581 ggtcgcgggc gcggattgac gatcgtatcg acgaattaat ggcgctactg gggctggagt
    47641 caaatttgcg tgagcgttat ccgcatcagc tttccggtgg tcagcagcaa cgtgtgggag
    47701 tggcgcgtgc actggctgcc gatccgcaag tcttactgat ggatgaacct tttggcgcac
    47761 tggacccggt aacgcgcggc gcgttgcaac aagagatgac gcgcattcac cgtttgctgg
    47821 ggcgcactat tgtgctggtc actcatgata ttgatgaggc gctacggctg gcagaacatc
    47881 tggtattgat ggatcacggt gaagtggtgc agcaggggaa tccgctgacg atgctgactc
    47941 gtccggcgaa tgattttgtc cgccagtttt ttggacgtag tgaactgggg gtgcacctgc
    48001 tttcgttacg tagcgtggcg gattacgtgc gtcgcgaaga acgggcagaa ggtgaggcac
    48061 tggcagaaga gatgacgcta cgcgatgcgc tctccctgtt tgtcgcgcgg ggatgcgagg
    48121 tgctgccggt ggtgaacacg cagggccagc cttgcggcac gctgcatttt caggatctgc
    48181 tggtggaggc gtaagcgtat gaagatgttg cgcgatccgc tgttctggct cattgctctt
    48241 tttgtggcgc tgattttctg gctgccttac agccagccgc tgtttgctgc cttgttccca
    48301 caactgccac gacccgttta tcagcaagaa agttttgcag ctctggcact ggctcatttc
    48361 tggctggtgg gaatttcgag tttgtttgcg gtgatcattg gcacaggtgc cggaattgct
    48421 gttactcgcc cgtggggcgc ggaatttcgc ccactggtgg aaactattgc cgccgttgga
    48481 cagacttttc cgcctgtcgc agtgctggcg attgccgttc cggtgatcgg ctttggtctg
    48541 caaccagcga ttatcgcctt gatcctttac ggtgtgctgc ccatcctgca ggtgacactt
    48601 gccgggctgg gagcgattga tcccagcgtg acagaagttg cgaaaggtat gggaatgagt
    48661 cgaggtcagc gactgcgtaa ggtcgagcta ccgctggcgg ctccggtgat tctggcgggc
    48721 gtgcgaactt cggtgattat caacattggt acggcgacga tcgcctcaac ggtaggggcc
    48781 agtacgctgg gtacgcccat catcatcggg cttagcggat ttaataccgc gtatgtgatc
    48841 cagggggcgt tactggtggc actggcggcg atcatcgcag accgcctgtt tgaaaggctg
    48901 gtgcaggcgc ttagccagca cgcaaaataa aggtataacc tgcgagcatg acgccaccaa
    48961 ttccgcctaa cgccataaac aggaacaggg cgatgacccc aattttagct atgcgcataa
    49021 tgcactcctt atgttaacga aaggattgta cagtaaagcg catttgttaa cgaatcatta
    49081 aataccgagt gggaaaatat catggccttg ttcttgccaa ctggtgagtt gctgctgttg
    49141 ggcggaggtt cgattttcac cgcaccacac cagcaatgta cggccttcga atagttcagg
    49201 gcgtagttga ttgagcgagt gggcgagcga ggacatcaat gcgccatcct tgttgactgg
    49261 caatccagcc ctccagccac agacgggtgg tatcctgaat attccagcca accaccagcg
    49321 catctttacc ctgtttttta cgtgccgaag ccagacaaat ggcgatgtag ttgatcagta
    49381 cgccgtcgag gatcgccagc agcgcctgga gagtcggttg ttggcactga agccgtcggc
    49441 gcagaggaat aaacagatgt gtggtgagtg tctgggcggg gtaatcctga ccgcgctctt
    49501 tgatccacgt tcgcaggcta tgtagattgc cg
//
LOCUS       DAZSIO010000010        48439 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZSIO010000010 DAZSIO010000000
VERSION     DAZSIO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.010"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            330..1547
                     /gene="alaA"
                     /locus_tag="ACHX5T_000486"
     CDS             330..1547
                     /gene="alaA"
                     /locus_tag="ACHX5T_000486"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR8465819.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1631..2230
                     /gene="yfbR"
                     /locus_tag="ACHX5T_000487"
     CDS             1631..2230
                     /gene="yfbR"
                     /locus_tag="ACHX5T_000487"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR8465820.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2289..4121)
                     /locus_tag="ACHX5T_000488"
     CDS             complement(2289..4121)
                     /locus_tag="ACHX5T_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR8465821.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4208..4858)
                     /gene="hxpA"
                     /locus_tag="ACHX5T_000489"
     CDS             complement(4208..4858)
                     /gene="hxpA"
                     /locus_tag="ACHX5T_000489"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFR8465822.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4869..5363)
                     /locus_tag="ACHX5T_000490"
     CDS             complement(4869..5363)
                     /locus_tag="ACHX5T_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR8465823.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5446..5901)
                     /gene="yfbV"
                     /locus_tag="ACHX5T_000491"
     CDS             complement(5446..5901)
                     /gene="yfbV"
                     /locus_tag="ACHX5T_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR8465824.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6239..7441
                     /gene="ackA"
                     /locus_tag="ACHX5T_000492"
     CDS             6239..7441
                     /gene="ackA"
                     /locus_tag="ACHX5T_000492"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR8465825.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7516..9660
                     /gene="pta"
                     /locus_tag="ACHX5T_000493"
     CDS             7516..9660
                     /gene="pta"
                     /locus_tag="ACHX5T_000493"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR8465826.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9850..11370
                     /gene="yfcC"
                     /locus_tag="ACHX5T_000494"
     CDS             9850..11370
                     /gene="yfcC"
                     /locus_tag="ACHX5T_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR8465827.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11403..11945)
                     /gene="yfcD"
                     /locus_tag="ACHX5T_000495"
     CDS             complement(11403..11945)
                     /gene="yfcD"
                     /locus_tag="ACHX5T_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR8465828.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12003..12554)
                     /gene="yfcE"
                     /locus_tag="ACHX5T_000496"
     CDS             complement(12003..12554)
                     /gene="yfcE"
                     /locus_tag="ACHX5T_000496"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR8465829.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12610..13254)
                     /gene="yfcF"
                     /locus_tag="ACHX5T_000497"
     CDS             complement(12610..13254)
                     /gene="yfcF"
                     /locus_tag="ACHX5T_000497"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR8465830.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13390..14037
                     /gene="yfcG"
                     /locus_tag="ACHX5T_000498"
     CDS             13390..14037
                     /gene="yfcG"
                     /locus_tag="ACHX5T_000498"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR8465831.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14094..14456
                     /gene="folX"
                     /locus_tag="ACHX5T_000499"
     CDS             14094..14456
                     /gene="folX"
                     /locus_tag="ACHX5T_000499"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR8465832.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14477..15370
                     /locus_tag="ACHX5T_000500"
     CDS             14477..15370
                     /locus_tag="ACHX5T_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR8465833.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15418..16308)
                     /gene="rpnB"
                     /locus_tag="ACHX5T_000501"
     CDS             complement(15418..16308)
                     /gene="rpnB"
                     /locus_tag="ACHX5T_000501"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR8465834.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16505..17278)
                     /gene="hisP"
                     /locus_tag="ACHX5T_000502"
     CDS             complement(16505..17278)
                     /gene="hisP"
                     /locus_tag="ACHX5T_000502"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR8465835.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17286..18002)
                     /locus_tag="ACHX5T_000503"
     CDS             complement(17286..18002)
                     /locus_tag="ACHX5T_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8465836.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17999..18685)
                     /gene="hisQ"
                     /locus_tag="ACHX5T_000504"
     CDS             complement(17999..18685)
                     /gene="hisQ"
                     /locus_tag="ACHX5T_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR8465837.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18775..19557)
                     /gene="hisJ"
                     /locus_tag="ACHX5T_000505"
     CDS             complement(18775..19557)
                     /gene="hisJ"
                     /locus_tag="ACHX5T_000505"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR8465838.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19778..20560)
                     /gene="argT"
                     /locus_tag="ACHX5T_000506"
     CDS             complement(19778..20560)
                     /gene="argT"
                     /locus_tag="ACHX5T_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR8465839.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20826..21395)
                     /gene="ubiX"
                     /locus_tag="ACHX5T_000507"
     CDS             complement(20826..21395)
                     /gene="ubiX"
                     /locus_tag="ACHX5T_000507"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR8465840.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21490..23007)
                     /gene="purF"
                     /locus_tag="ACHX5T_000508"
     CDS             complement(21490..23007)
                     /gene="purF"
                     /locus_tag="ACHX5T_000508"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR8465841.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23044..23532)
                     /gene="cvpA"
                     /locus_tag="ACHX5T_000509"
     CDS             complement(23044..23532)
                     /gene="cvpA"
                     /locus_tag="ACHX5T_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR8465842.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23791..24453)
                     /gene="dedD"
                     /locus_tag="ACHX5T_000510"
     CDS             complement(23791..24453)
                     /gene="dedD"
                     /locus_tag="ACHX5T_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR8465843.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24443..25711)
                     /gene="folC"
                     /locus_tag="ACHX5T_000511"
     CDS             complement(24443..25711)
                     /gene="folC"
                     /locus_tag="ACHX5T_000511"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR8465844.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25781..26695)
                     /gene="accD"
                     /locus_tag="ACHX5T_000512"
     CDS             complement(25781..26695)
                     /gene="accD"
                     /locus_tag="ACHX5T_000512"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR8465845.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26851..27510)
                     /locus_tag="ACHX5T_000513"
     CDS             complement(26851..27510)
                     /locus_tag="ACHX5T_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR8465846.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27593..28405)
                     /gene="truA"
                     /locus_tag="ACHX5T_000514"
     CDS             complement(27593..28405)
                     /gene="truA"
                     /locus_tag="ACHX5T_000514"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR8465847.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28405..29418)
                     /locus_tag="ACHX5T_000515"
     CDS             complement(28405..29418)
                     /locus_tag="ACHX5T_000515"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR8465848.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29484..30620)
                     /gene="pdxB"
                     /locus_tag="ACHX5T_000516"
     CDS             complement(29484..30620)
                     /gene="pdxB"
                     /locus_tag="ACHX5T_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR8465849.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30719..31714
                     /gene="flk"
                     /locus_tag="ACHX5T_000517"
     CDS             30719..31714
                     /gene="flk"
                     /locus_tag="ACHX5T_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR8465850.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31711..32889)
                     /locus_tag="ACHX5T_000518"
     CDS             complement(31711..32889)
                     /locus_tag="ACHX5T_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8465851.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33144..34385)
                     /gene="fabB"
                     /locus_tag="ACHX5T_000519"
     CDS             complement(33144..34385)
                     /gene="fabB"
                     /locus_tag="ACHX5T_000519"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR8465852.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            34544..36550
                     /gene="mnmC"
                     /locus_tag="ACHX5T_000520"
     CDS             34544..36550
                     /gene="mnmC"
                     /locus_tag="ACHX5T_000520"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR8465853.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36671..36949)
                     /locus_tag="ACHX5T_000521"
     CDS             complement(36671..36949)
                     /locus_tag="ACHX5T_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR8465854.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(36983..37531)
                     /gene="epmC"
                     /locus_tag="ACHX5T_000522"
     CDS             complement(36983..37531)
                     /gene="epmC"
                     /locus_tag="ACHX5T_000522"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR8465855.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37531..38340)
                     /locus_tag="ACHX5T_000523"
     CDS             complement(37531..38340)
                     /locus_tag="ACHX5T_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFR8465856.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38340..39164)
                     /gene="mepA"
                     /locus_tag="ACHX5T_000524"
     CDS             complement(38340..39164)
                     /gene="mepA"
                     /locus_tag="ACHX5T_000524"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR8465857.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39168..40253)
                     /gene="aroC"
                     /locus_tag="ACHX5T_000525"
     CDS             complement(39168..40253)
                     /gene="aroC"
                     /locus_tag="ACHX5T_000525"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR8465858.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40288..41220)
                     /gene="prmB"
                     /locus_tag="ACHX5T_000526"
     CDS             complement(40288..41220)
                     /gene="prmB"
                     /locus_tag="ACHX5T_000526"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR8465859.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            41386..41937
                     /gene="smrB"
                     /locus_tag="ACHX5T_000527"
     CDS             41386..41937
                     /gene="smrB"
                     /locus_tag="ACHX5T_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR8465860.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42041..42931)
                     /locus_tag="ACHX5T_000528"
     CDS             complement(42041..42931)
                     /locus_tag="ACHX5T_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR8465861.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(42918..43442)
                     /locus_tag="ACHX5T_000529"
     CDS             complement(42918..43442)
                     /locus_tag="ACHX5T_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8465862.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(43439..43909)
                     /locus_tag="ACHX5T_000530"
     CDS             complement(43439..43909)
                     /locus_tag="ACHX5T_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8465863.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(43906..44454)
                     /locus_tag="ACHX5T_000531"
     CDS             complement(43906..44454)
                     /locus_tag="ACHX5T_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8465864.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(44459..45181)
                     /locus_tag="ACHX5T_000532"
                     /pseudo
     CDS             complement(44459..45181)
                     /locus_tag="ACHX5T_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281619.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(45201..47841)
                     /gene="yfcU"
                     /locus_tag="ACHX5T_000533"
                     /pseudo
     CDS             complement(45201..47841)
                     /gene="yfcU"
                     /locus_tag="ACHX5T_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(47923..48438)
                     /locus_tag="ACHX5T_000534"
     CDS             complement(47923..48438)
                     /locus_tag="ACHX5T_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8465865.1"
                     /translation="MFTSTATIAAGNNGTARFYGTIEDSVCSIVPDDHKLEVDMGDIG
                     AEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSANSGNYYTIFNTDTGAAF
                     NNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNFNAWLVGAADAPDLGNF
                     EANTTFQITYL"
BASE COUNT        12010 a        13016 c        11961 g        11452 t
ORIGIN      
        1 tcacgctgtt aaactctccg taatgcggaa atatcatcat cgcgtttcat tagtgaattc
       61 ttaaatgagg cattttcaca caattatctt acagataaaa aaaccagact tgcaattaag
      121 aattagaaca attcactata taacattgca tgtaaagcat ataagcctaa ttatttgtca
      181 ttattaagtt attaacagca caatcgagcc ttcccctctg gcaaaatctt attctgcaga
      241 ccttcaaaac accgtcctgg gggagtacat tgttctaagc tgacttccac ggcagggagt
      301 ggcgataaca gcaaaaaagg tcaagattca tgtcccccat tgaaaaatcc agcaaattag
      361 agaatgtctg ttatgacatc cgtggtccgg tgctgaaaga agcaaaacgc ctggaagaag
      421 aaggtaacaa ggtactgaaa ctgaacatcg gcaacccagc cccattcggt tttgacgcgc
      481 cagatgaaat cctcgttgac gtgatacgca acctgcctac cgctcaaggg tattgcgatt
      541 ccaaagggct ttactccgcg cgtaaagcca tcatgcagca ctaccaggct cgtggcatgc
      601 gtgatgttac cgtggaagat atttacatcg gcaatggtgt atcggagctt atcgttcagg
      661 caatgcaggc attgctgaac agcggggacg aaatgttggt tcctgcacca gattacccac
      721 tctggaccgc ggcggtttcg ctttccagcg gtaaagcggt gcattatctt tgcgatgaat
      781 cctctgactg gttcccggac ctcgatgata ttcgcgctaa aattacgcct cgtacgcgtg
      841 ggattgttat tatcaaccca aataacccaa ccggcgcggt atattccaaa gagcttttaa
      901 tggagattgt ggagattgca cgtcagcata atctcattat cttcgccgat gaaatttatg
      961 acaaaattct ctacgacgat gctgagcatc actcaattgc gccgctggca cctgacctgc
     1021 tgaccattac ctttaacgga ctgtcgaaaa cgtaccgcgt tgcaggcttc cgtcaggggt
     1081 ggatggtgtt gaacgggccg aaaaaacacg ccaaaggcta catcgaaggt ctggaaatgc
     1141 tggcttcaat gcgcctgtgt gctaacgttc ctgcgcaaca cgccattcag accgcgctgg
     1201 gtggttatca gagcatcagt gaatttatta cccctggcgg tcgtctttat gagcagcgta
     1261 accgcgcgtg ggaactgatc aacgatattc cgggcgtttc ctgcgtgaaa cctcgtggtg
     1321 cgctgtatat gttcccgaaa atcgacgcca aacgctttaa cattcatgac gatcagaaaa
     1381 tggtgctgga tttcctgttg caggaaaaag ttctgttggt gcaagggacg gcattcaact
     1441 ggccgtggcc ggatcacttc cgcattgtca cgctaccgcg tgtcgatgat atcgagctgt
     1501 ctttgagcaa gttcgcgcgt ttcctttctg gttatcatca gctgtaatct taatttcact
     1561 gccggagatt gcatccggca gtgttatccc gccacaatga cctgatgatg tcatcatacg
     1621 taaggtcact atgaaacaga gccatttttt tgcccatctc tcccgcctga aactcattaa
     1681 ccgctggccg ctaatgcgca acgtgcggac ggaaaatgtg tccgaacaca gtttgcaggt
     1741 agcgatggtc gcccatgcgc tggcagctat caaaaatcgg aaatttggcg gtaatgtcaa
     1801 cgccgaacgt atcgctttac tggcgatgta ccacgatgcc tcagaagtgc tcaccggcga
     1861 tctccctact ccggtgaaat acttcaatgc gcaaatcgct caggagtaca aggctattga
     1921 aaaaatcgct cagcagaaac tggtcgatat ggttccggaa gaactgcggg atatctttgc
     1981 gccgttaatt gacgagcatg catatagcga tgaagaaaaa tcgctggtga aacaggcaga
     2041 tgcactgtgt gcatatctga aatgtctgga agaactcgcg gccggaaata atgaattctt
     2101 gctggcaaaa acgcgactgg aagcgacgct tgaagcgcgt cgcagccagg agatggacta
     2161 cttcatggaa gtatttgttc ccagcttcca tctttcgctc gatgagatta gccaggattc
     2221 accgctgtaa gcagccggag tctgcgtcgc atcagtcaat aagcgccgga tgcgacatca
     2281 ggctcttgtc aaaacggaaa cagcatcggg atcatcacca cacaaaccgc catcacgata
     2341 atggtgaacg gtacccccaa cttcacaaag tcactgaagc tgtaatttcc cggacctaaa
     2401 accagggtgt taacaggtga agacaccggt gtcataaagg cggcggatgc tgccatcgcc
     2461 acgaccatcg caaatggata aggcgacact cccatcgttt tggcagcagc cagcgcaatc
     2521 ggagccatca acaccgccgt cgcggtatta gagataaaca gcccaataac cgccgacaag
     2581 acaaacaaac accccagcat catatgtggc ccataaccgc cgccaatatc catcagccct
     2641 ttcaccgcca gcgcgacacc tcccgttttc tgtaatgcca cagcaaacgg catcatccca
     2701 acgatcaaaa taatgctcgg ccagtgaatg gatttatagg cgctttcagc atctatacag
     2761 cggaatttcc ccatcagcag gcaggcgatg atagcggcaa caggattagg aatttcattt
     2821 gtcagcatta acgccaccat tagcaccaga cagaaaatgg catggggtgc ctggctgtgc
     2881 gcgggcgatg cttcactcac ctcttccggt aagttcagcg ctacgaagtc gcggccctgt
     2941 ttggccagca taccgatcag tttccagtta cccacaacca ggatgatatc gcccagcagc
     3001 agaggctcat ccgccagcga accttccagc gccacgccat tgcgcttgag ccccaccaca
     3061 ttcagtccgt agcgggtacg aaaaccaatt tcgcgcaccg atttaccaat cagttctgac
     3121 tcaggaatta atgaaatctc tgccatgccc acatcaaggg cctggtcaga aaaatactcg
     3181 ccgcgcagta ccatcggctc cagcaattgc tcactacaaa attgccggag atcgacatca
     3241 gccgcagaca tatcaataag caaaacgtca cgcgcgcgaa attcagaaac cccattaacg
     3301 ttcacgataa cgcgacgaaa acgccgccag cgttcaacac cgatgacgtt agcgccataa
     3361 cgctcacgta atttgagatc atccagccgt tgacctatca ttggcgatcc ggggcgaata
     3421 gccagacgtc gcgcacgccc ggtcagtcga tattcacgga taagatcgcg aaaggttcga
     3481 cgcgtccagc cttcgcgctg cggggtctgg gtatcccctt tcagcatgaa acgcatcact
     3541 aacatataca aaatacccag caccagcacg accaggccaa taggtgttac gctaaagaaa
     3601 ctgaagccgt gatagccttc acgcagcaat tcactgttga ctaccaggtt cggcggcgtc
     3661 gccaccagcg tcatcatgcc gctaatcagc ccggcaaaac ttaacggcat catcagacgt
     3721 gacggcgaag tttgcatacg catggcaacg cttaacacca cgggaataaa gatagcgaca
     3781 acgctggttg aactcataaa cgcgccaagc cccgcgacgg tcagcatcaa caaaaccaac
     3841 attttgattt cactgttgcc cgccactttg accagccatg ttcccattac ggtggcaaca
     3901 ccggtacgga ccaaaccatc gccaataata aacaaggcgg caatcaggac aacgttagga
     3961 tcagaaaagc cggaaaatac ttcggggacc gtcagcgttc cgcttaatgc aaacgcgaca
     4021 ataacaaaca aagcgaccgc atccatacgc actctgcccg tcgcaaacaa gacgatggca
     4081 acggccagta atgaaagaac ccaaatcaat tcaccgttca caacttatcc ttgttaattg
     4141 agggggatga cttgattctg ccataaaaaa gccccgacga gacggggcta aatcatgatc
     4201 aggtgtttca ctgaataata acatcgccat ttggctgttt ggtcacagta atttgctcca
     4261 gactgtggag gaccaaatcg acctcattca ggcgcggggt atctgccgga gcgttaaccg
     4321 caatgacatg gcaacccgcc gccaggccag aaagcacgcc agcgggagca tcttccacca
     4381 ccacacactc ctgcggcgca agccccagca gctgcgcacc taacagatac gcatcaggtt
     4441 ctggttttcc gcgcttcact cgctcagcgg ttacaaacac ctctggtgcg ggaagccccg
     4501 ctattttatg gcgcgctcgc gctaccggca tggagccaga agtcacaatg gcccacggaa
     4561 tacctgcttt attcaaatga ttgagtaagg cgattgcccc aggaagcgcg gtaatacctt
     4621 cggtttccgt ggcctcgatt tgctccagac gcgtaaactc ggcggcaata tcagcctcgg
     4681 atttgcccgc cataaaatgg cgcaaagagg tgatcgcctg tttaccgtga atgaaagcca
     4741 gcacctcttc cggcgctaac ccatgacgtc tggcccagtt gctccacgcc cgttctaccg
     4801 caggcaggga atccaccagc gttccatcaa gatcaaacag aaaacctttg caccgcacgc
     4861 gggcctcctc aggcgttgat aatttgatta atttcgttgg cgctcaaatg gtactgacgc
     4921 gggcaggcat gccacacatt aagcatgcgc tgatattttt cccacattgg cgtctgggcg
     4981 ttaaaaccat gagtcccagc gtcaaaatgg gtatagcgcc cttccacatt aaccataaag
     5041 cggacataac cgaggtaacg tgcttcagtg gcggcgtcaa agccgaggaa ggtgacacga
     5101 cgttcatcga tggattgctg gtcctgcaaa ttagaccaag aaacatacaa cgcatgatac
     5161 atatccataa tgtcgatgat agtgcggcag gtttcttctt tcagctcgcc aaactcgcga
     5221 tccaattcac gcatctgtaa tccgtaacca cgctcaataa ttgtttgcag gcgacggtaa
     5281 cgttcagcat ttgccggatc gagcatagtc atcatcttgt actgattaga caaaataaga
     5341 cgttgcgcgt tggtcatttc cattgttgac tcctgtatca ctctactacg gtgaaaaaaa
     5401 agaaggctga gtatgccttc ttttatatgc gtaatcaggg gtcaattaca aatcatcaag
     5461 gaaagtttta tccagttgtt tgaaggcgcg cttaagcgtg tcagctaatg cctggtaatc
     5521 aggcttgcct tcaacgggtg ccaacacctg tccagactcc tgcaatttac cgcgaacttc
     5581 ataaaaccag ttaaggattg cagggggtaa tggcgttaca gaacgcttgc ccagccacca
     5641 caatccctgc atgggtaaac ttaaggcgaa cagtgcagtg gcaactgccg gcccaagcag
     5701 accgcccagg gcaatctgcc agcagagagt aaatacggcg atcggcggca taaaacggat
     5761 cgcataacgc gtcatcttga taacgcgatt ttcgacaaag accggagcaa ggcgtttttc
     5821 cagtggccac gtctttgagt aatgctgtcc gcggcgaaac aagctaaaaa aattaacaga
     5881 acgattatcc ggcgttgaca tgcttcacct caacttcaca tataaagatt caaaaatttg
     5941 tgcaaattca caactcagcg ggacaacgtt caaaacattt tgtcttccat acccactatc
     6001 aggtatcctt tagcagcctg aaggcctaag tagtacatat tcattgagtc gtcaaattca
     6061 tatacattat gccattggct gaaaattacg caaaatggca tagactcaag atatttcttc
     6121 catcatgcaa aaaaaatttg cagtgcatga tgttaatcat aaatgtcggt gtcatcatgc
     6181 gctacgctct atggctccct gacgtttttt tagccacgta tcaattatag gtacttccat
     6241 gtcgagtaag ttagtactgg ttctgaactg cggtagttct tcactgaaat ttgccatcat
     6301 cgatgcagta aatggtgaag agtacctttc tggtttagcc gaatgtttcc acctgcccga
     6361 agcacgtatc aaatggaaaa tggacggcaa taaacaggaa gcggctttag gtgcaggcgc
     6421 cgctcacagc gaagcgctca actttatcgt taatactatt ctggcacaaa aaccagaact
     6481 gtctgcgcag ctgactgcta tcggtcaccg tatcgtacac ggcggcgaaa agtataccag
     6541 ctccgtagtg atcgatgagt ctgttattca gggtatcaaa gatgcagctt cttttgcacc
     6601 gctgcacaac ccggctcacc tgatcggtat cgaagaagct ctgaaatctt tcccacagct
     6661 gaaagacaaa aacgttgctg tatttgacac cgcgttccac cagactatgc cggaagagtc
     6721 ttacctctac gccctgccgt acaacctgta caaagagcac ggcatccgtc gttacggcgc
     6781 gcacggcacc agccacttct atgtaaccca ggaagcggca aaaatgctga acaaaccggt
     6841 agaagaactg aacatcatca cctgccacct gggcaacggt ggttccgttt ctgctatccg
     6901 caacggtaaa tgcgttgaca cctctatggg tctgaccccg ctggaaggtc tggtcatggg
     6961 tacccgttct ggtgatatcg atccggcgat catcttccac ctgcacgaca ccctgggcat
     7021 gagcgttgac gcaatcaaca aactgctgac caaagagtct ggcctgctgg gtctgaccga
     7081 agtgaccagc gactgccgct atgttgaaga caactacgcg acgaaagaag acgcgaagcg
     7141 cgcaatggac gtttactgcc accgcctggc gaaatacatc ggtgcctaca ctgcgctgat
     7201 ggatggtcgt ctggacgctg ttgcattcac tggtggtatc ggtgaaaatg ccgcgatggt
     7261 tcgtgaactg tctctgggca aactgggcgt gctgggcttt gaagttgatc atgaacgcaa
     7321 cctggctgca cgtttcggca aatctggttt catcaacaaa gaaggtaccc gtcctgcggt
     7381 ggttatccca accaacgaag aactggttat cgcgcaagac gcgagccgcc tgactgcctg
     7441 atttcacacc gccagctcag ctggcggtgc tgttttgtaa cccgccaaat cggcggtaac
     7501 gaaagaggat aaaccgtgtc ccgtattatt atgctgatcc ctaccggaac cagcgtcggt
     7561 ctgaccagcg tcagccttgg cgtgatccgt gcaatggaac gcaaaggcgt tcgtctgagc
     7621 gttttcaaac ctatcgctca gccgcgtacc ggtggcgatg cgcccgatca gactacgact
     7681 atcgtgcgtg cgaactcttc caccacgacg gccgctgaac cgctgaaaat gagctacgtt
     7741 gaaggtctgc tttccagcaa tcagaaagat gtgctgatgg aagagatcgt cgcaaactac
     7801 cacgctaaca ccaaagacgc tgaagtcgtt ctggttgaag gtctggtccc gacacgtaag
     7861 caccagtttg cccagtctct gaactacgaa atcgctaaaa cgctgaatgc ggaaatcgtc
     7921 ttcgttatgt ctcagggcac tgacaccccg gaacagctga aagagcgtat cgaactgacc
     7981 cgcaacagct tcggtggtgc caaaaacacc aacatcaccg gcgttatcgt taacaaactg
     8041 aacgcaccgg ttgatgaaca gggtcgtact cgcccggatc tgtccgagat tttcgacgac
     8101 tcttccaaag ctaaagtaaa caatgttgat ccggcgaagc tgcaagaatc tagcccgctg
     8161 ccggttctcg gcgctgtgcc gtggagcttt gacctgatcg cgactcgtgc gatcgatatg
     8221 gctcgccacc tgaatgcgac catcatcaac gaaggcgaca tcaatactcg ccgcgttaaa
     8281 tccgtcactt tctgcgcacg cagcattccg cacatgctgg agcacttccg tgccggttct
     8341 ctgctggtga cttccgcaga ccgtcctgac gtgctggtgg ccgcttgcct ggcagccatg
     8401 aacggcgtag aaatcggtgc cctgctgctg actggcggct acgaaatgga cgcgcgcatt
     8461 tctaaactgt gcgaacgtgc tttcgctacc ggcctgccgg tatttatggt gaacaccaac
     8521 acctggcaga cctctctgag cctgcagagc ttcaacctgg aagttccggt tgacgatcac
     8581 gaacgtatcg agaaagttca ggaatacgtt gctaactaca tcaacgctga ctggatcgaa
     8641 tctctgactg ccacttctga gcgcagccgt cgtctgtctc cgccagcgtt ccgttatcag
     8701 ctgactgaac tggcgcgcaa agcgggcaaa cgtatcgttc tgccggaagg tgacgaaccg
     8761 cgtaccgtta aagcagccgc tatctgtgct gaacgtggta tcgcaacttg cgtactgctg
     8821 ggtaatccgg cagagatcaa ccgtgttgca gcgtctcagg gtgtagaact aggtgcaggc
     8881 attgaaatcg ttgatccaga agtggttcgc gaaagctatg ttggtcgtct ggtcgagctg
     8941 cgtaagaaca aaggcatgac cgaaaccgtt gcccgcgaac agctggaaga caacgtggtg
     9001 ctcggtacgc tgatgctgga acaggatgaa gttgatggtc tggtttccgg tgctgttcac
     9061 actaccgcaa acaccatccg tccgccgctg cagctgatca aaactgcacc gggcagctcc
     9121 ctggtatctt ccgtgttctt catgctgctg ccggaacagg tttacgttta cggtgactgt
     9181 gcgatcaacc cggatccgac cgctgaacag ctggcagaaa tcgcgattca gtccgctgat
     9241 tccgctgcgg ccttcggtat cgaaccgcgc gttgctatgc tctcctactc caccggtact
     9301 tctggtgcag gtagcgacgt agaaaaagtt cgcgaagcaa ctcgtctggc gcaggaaaaa
     9361 cgtcctgacc tgatgatcga cggtccgctg cagtacgacg ctgcggtaat ggctgacgtt
     9421 gcgaaatcca aagcgccgaa ctctccggtt gcaggtcgcg ctaccgtgtt catcttcccg
     9481 gatctgaaca ccggtaacac cacctacaaa gcggtacagc gttctgccga cctgatctcc
     9541 atcgggccga tgctgcaggg tatgcgcaaa ccggttaacg acctgtcccg tggcgcactg
     9601 gttgacgata tcgtctacac catcgcgctg actgcgattc agtctgcaca gcagcagtaa
     9661 tctcgtcatc atccacagct ttgcgctgcg gatatctgaa ccggaaataa tcactatttc
     9721 cggtttttta ttctcttaat ttgcattaat cctttctgat tatcttgctt aactgcgctg
     9781 catcaatgaa ttgcgccatc tcactttgca tacttaccac tttgttttgt gcaagggaat
     9841 atttgcgcta tgtccgcaat cactgaatcc aaaccaacaa gaagatgggc aatgcccgat
     9901 acgttggtga ttatcttttt tgttgctatt ttaaccagtc ttgccacctg ggtagttccg
     9961 gtggggatgt ttgacagtca ggaagtgcag tatcaggttg atggtcaaac aaaaacacgc
    10021 aaagtcgtag atccacactc atttcgcatt ctgactaacg aagcaggcga acctgagtat
    10081 caccgcgtac agctgttcac gacgggcgat gaacgcccgg gcctgatgaa cttcccgttt
    10141 gaagggttaa cctcaggatc gaaatacggg acagccgttg gcatcatcat gtttatgctg
    10201 gtgattggcg gcgcgtttgg cattgtgatg cgtacaggaa ccattgataa cggtatcctg
    10261 gcgcttattc gccatacccg cgggaatgaa attctcttta ttcctgcgct gtttattctg
    10321 ttttcacttg gcggcgcggt atttggtatg ggagaagagg ccgtcgcctt tgccattatc
    10381 atcgcaccgc taatggtccg gctgggctat gacagtatta ccaccgtcct ggtgacttat
    10441 attgccacgc aaatcggttt tgccagttcg tggatgaacc cgttttgtgt ggttgttgct
    10501 caggggattg ccggcgttcc ggtgctttct ggctccgggt tgcgcatcgt ggtgtgggtt
    10561 atcgccactc tgattggcct gatctttacc atggtgtacg cctcacgagt gaaaaagaat
    10621 cctcttctgt cacgcgtgca tgagtccgac cgcttctttc gtgaaaagca ggcggatgtt
    10681 gaacaacgtc cgtttacctt tggtgactgg ctggtattga ttgtcctgac agccgtaatg
    10741 gtctgggtga tttggggcgt gatcgttaat gcctggttta ttccagaaat tgccagccag
    10801 ttcttcacca tgggtctggt gattggcatc atcggcgttg ttttccgcct taacggcatg
    10861 acggttaata ccatggcttc atcctttacc gaaggggcgc gaatgatgat cgcccctgcc
    10921 ctgctggtgg gtttcgccaa agggattttg ctgctggttg gtaatggtga agcaggtaat
    10981 gccagcgtgt taaatactat cctcaacagc attgccaatg ccattagcgg tctggacaac
    11041 gcggtcgcgg cctggtttat gttgctcttc caggctgtat ttaatttctt cgtgacgtcc
    11101 ggttctggtc aggcggcatt aactatgccg ttactggcac cgcttggcga tctggtcggt
    11161 gttaaccgtc aggttaccgt gctggcgttc cagtttggtg atggcttcag ccacatcatt
    11221 tacccaacct cagcttcgtt aatggcaacg ctcggtgtgt gccgggtgga cttccgtaac
    11281 tggctgaagg tgggcgcgac cctgcttgga ctgctgttta ttatgtccag cgtcgtagtg
    11341 atcggcgctc agttgatggg ctaccactaa aaatgttaag agccgcaatt gcggctcttt
    11401 tttcattctg ccgtttcagt ctctacggct tcatttttgg cattgcgctt catccacaac
    11461 gccagcgctt tcagcgagtc tggagtgaac tcatcgcagc gtgcggtgat ttcttccggc
    11521 gtcagccagc aaactccact gacttcatct tcctgtaggg cgaagggacc gtgagagacg
    11581 caactgaaca acgcgcccca gacacggcaa tttttatctt cgaaatagaa ctgcccgtgc
    11641 tcggcaaagg ggacaccggc aatgcccaac tcttcttccg cttcgcgacg cgcggattcc
    11701 agcagttgct catcggcctg aactacaccg cctgcggtcg catctaacat gccgggtaaa
    11761 aagtcttttg tctcggtacg acgctggacc agaattttgc ccatgccatc atgcacgacg
    11821 atgtaagttg cacgatgacg cagacactgt gcccgcattt gttcccggct ggcttgtgca
    11881 atgacttcgt tctcttcatt gacaatatcc acccattcag tacttgccaa acgacgctgt
    11941 tccattatca ggaaaccttc tttttctggc gctcttacgg cgcattttga gttgtgggta
    12001 aattacggat taatcgcgac ctgcgcaatg atactttgat cattgagtgc gattacacta
    12061 agtacgtcat tatccagcat gccataaccc gccggattac cgcctttcgg aatactcacc
    12121 gagccggggt tgaagtggaa aatctccccc cgctgttctg ccactggcag atgggtatga
    12181 ccgtacacca gcacatcgtt ctggtttaaa gcaggtagat tttccgggcc aaaaagatga
    12241 ccatgcgtca aaaacagacg ttgtttttcc agtaatacct gttgccacgg cgcggttatc
    12301 gggaaatgca gcatcatttg atccacttcg ctgtcgcagt tgccacgcac agcgataacc
    12361 ttatgtgcca cttcattaag ccgttcagcg actttggctg gcgcgtaacc ctccggtaaa
    12421 gcattacgcg ggccgtgatt caacacgtcg ccaaggatca ccagccactg ggcaccgctt
    12481 tggacaaaca actccacaac acgttccgtc gccggtaacg acccatgaat gtccgatgca
    12541 aacatcagtt tcatcactca ctcctcgtcg aaaaaacgta agctatgata ctggattctg
    12601 tcgttgctat cagccagatt gcttcgccga aagtgcaata aaacgctgga cagacgctcg
    12661 ctgccactgg aatgtcgcat aatccaccag gcgttctggc acctcatcgc catgtagtac
    12721 caggcggtta atcattagcg ccagatcagt atcagcaatg caccattcac caaataaatt
    12781 cggctgaccc agtgctaaca aatgttctgc catcgcgaac agtttctctg cactggcttt
    12841 tccctcggcc gttagtggcg cttttttcgc ccccgcaaag acaacatccg tcggacgctc
    12901 ttcgcggatg ggcatcagat cgctgcgcag ccaggcctga atctgtcgtg cacgcgcacg
    12961 attttctaaa tcaagcggat aaatacgttc ccaggtcggt ggcgcaaatc gatcttccag
    13021 atactccgca atggcagaag attcactcaa ctcaaaatca tcgatttgta ataacggcac
    13081 acggcgtgtc tgaccgtaac cttgccacgt cggctgcaaa tgttcaccgc tgtcgaggtc
    13141 gatggtcttg atatgaaacg acaggccttt ttcctgcaac gccacccagg cggataacac
    13201 ataaggggag aaaaagtggg catctgacca aagcgtgata gcgggtttac tcattatgtc
    13261 ctcggcaata aagggggctg gctatcaaac tatagagcct ttttgtcact gtcacgtgac
    13321 gaatactcac gtaagtcgcc gcgctttcta tacttgtggt gttcagatga ttaaacaacc
    13381 ggagctgcaa tgatcgatct ctatttcgcc ccgacaccca atggtcacaa aattacgctg
    13441 tttctcgaag aagcagagct tgattatcgc ttgattaagg tagacttggg gaaaggcggt
    13501 cagtttcgtc cggaattttt gcgcatttcg cccaacaaca aaattccggc aattgttgat
    13561 cattctcctg ccgatggcgg cgaaccgtta agcctctttg aggctgctgc cattttgttg
    13621 tatctggctg agaaaacagg actctttttg agtcatgaaa cgcgtgagcg cgccgccaca
    13681 ttacagtggt tattctggca ggtaggcgga ctggggccga tgcttgggca aaatcatcat
    13741 tttaatcacg cagcccccca aaccattcct tacgctattg aacgttatca ggttgaaact
    13801 cagcgtcttt accatgtact aaacaagcgg ctggaaaact cgccctggct gggaggcgag
    13861 aactacagca ttgcggatat tgcctgctgg ccgtgggtta atgcctggac tcgcctgcga
    13921 attgacctcg caatgtatcc ggcagtcaag aactggcatg agcggatccg ttcgcgccct
    13981 gccaccgggc aggcactgct aaaagcacaa ctcggtgatg agcgttcgga tagttaacag
    14041 aaacaggttc tcgtgtatta tttcatccta agtaaaacaa cggagaacct gcaatggcac
    14101 aacctgccgc tattattcgt ataaagaacc ttcgtttgcg tacgtttatc ggaattaagg
    14161 aagaagaaat taacaaccgt caggatattg ttatcaatgt gacgatccac taccccgccg
    14221 ataaagcgcg cactagcgaa gatatcaacg atgcgctgaa ttatcgcacc gtaacgaaaa
    14281 acattattca gcatgtagag aataaccgtt tctctttgct ggaaaaatta actcaggatg
    14341 tgctcgatat cgcacgtgaa catcactggg tgacgtatgc tgaagtggag atcgataaac
    14401 tgcacgcgct gcgctacgcc gattcggtat ccatgacctt aagctggcag cgttaatcgc
    14461 catatcggga ggctgcatga acatagtgat caccggaggg acgggattaa ttggtcgcca
    14521 tttgattccg cgtttgctgg agctgggcca tcaaattacg gtagtgacgc gtaacccgca
    14581 gaaagccagt tccgttctcg gccctcgggt gacactatgg caagggcttg ccgatcaaag
    14641 caacctcaac ggcgttgatg cggtaatcaa cctggccgga gaaccgattg ctgataaacg
    14701 ctggactcac aagcaaaaag agcgtctctg ccaaagccgc tggaatatca cgcaaaaact
    14761 ggtcgatttg attaatgcta gcgacacgcc gccgtcggta ctcatttccg gctcggcaac
    14821 gggctattat ggcgacttag gtgaagtggt ggttaccgaa gaggaaccgc cgcataacga
    14881 atttactcat aaactctgcg cccgctggga agagattgcc tgccgggcgc aaagtgacaa
    14941 aacgcgagtg tgcctgctgc gaaccggcgt ggtactggca ccggacggcg gtattctcgg
    15001 taaaatgctg ccaccgtttc gtcttggcct gggcgggccg attggttccg gtcggcagta
    15061 tctggcctgg attcatattg atgatatggt caacggcatt ctctggctgc tggataacga
    15121 gctgcgcggg ccatttaata tggtttcgcc ctacccggta cgcaatgaac aatttgccca
    15181 tgcgctcggt catgcgctgc atcgcccggc tattttgcgc gtccccacaa ccgccattcg
    15241 gctgttaatg ggcgaatctt cagtactggt attaggtgga caacgcgcgc tgcctaaacg
    15301 gctggaagaa gcgggttttg cgtttcgctg gtacgattta gaagaggcgc tggcggatgt
    15361 cgttcgctga tgtggtttac agcaaacatc cgccagttaa ctccctgtgt tacaggatta
    15421 gtggctttgc gcgataagat cgtctggtga aagtcgggtc accatcataa ctaactctct
    15481 gtctaaacct ctatccagca tctcctgagc aatacgcagg gcttcttcgt gtttgccctg
    15541 cattgcgcct tcttcacgta atctgtcagc aatggtcatc agtttctcct tttcttgtgg
    15601 tgcgcgttct gttatctcac caataaatgc acggaaacgc tgggcatccc ctgtttgtaa
    15661 tacgtaatta aacagggctt ttagctgtct gtcattagtg ttccctgtaa ctaacagcga
    15721 aacaatttgg tcgactaatc ccaacagatc gcgctgacga atatgtttct gaattaactc
    15781 caacagcgcc attttgcggt gttgcataat ctcgtcatcc ggcaccacgg taatatccac
    15841 caacggaaac gctgatgaat atattttgcg ggctatagca ggctcggcaa attcatcaag
    15901 ccagcagagt gaataaggat aaggacttct gcaaccatga taaaacagca tcgggatcac
    15961 caatggaagc tctttatagc ccgcatcaag atggttttgc attgccgcaa tggaataacg
    16021 catcatgcga aaagccatta attcttccgg cttactttgg tgctctatca ctacataaat
    16081 ataacccact ccctcctgcg ttttcacaga ccacaagagg tcggaataat attgccgcag
    16141 gtcttcatca ataaaactgt ttggttctag tttaagcgtc gttaaatcac acagtttgcg
    16201 cagcggcgcg ggaagatgaa tattaataaa attccgcgcg gtgtctggat ggcgtaaaaa
    16261 agatttaaat accgcatcat gcggcgtgga agttgtcgat attgtcatgg cgttccgtca
    16321 cccctcaaat ttaagatgac gcgacaataa ccacgattaa cacccaatgc agcaggcagt
    16381 ttttatcttt ggacagcgaa ttcagaagta tttttagcgg actgcaactt ttacatttca
    16441 gcggaagctg actcgcaaat tccccttgaa aatactccgg cagcgttagc caccggagag
    16501 gaaattattt cagcgatccc ttaaggaact gttgcagacg agggctttgc gggttgccaa
    16561 ataactgctc cggcgcgccc tcttcttcta ttttcccctg atggaggaaa atgacgtgag
    16621 tagaaacatg gcgagcaaag cccatttcgt gagtcactac caccatggtt ttcccctctt
    16681 ctgccagttg ctgcataata cgtaacactt cgcctaccag ttcaggatcg agcgccgagg
    16741 taggttcatc aaacagtaaa acttctggtt ccatcgccag cgcccgcgcg atagaaacac
    16801 gctgttgctg accgccggaa agatgcaccg gatatttccc ctgcgcacgt tcgtctatcc
    16861 cgactttcgc cagatacttc accgcccgct cgcgcgcttc ctgcttgctc aggcccaaca
    16921 cctgaatcgg cgcttccatg acgttttcca acaccgtcat atggctccag agattgaagt
    16981 gctggaatac catcgtcagg cgtgtgcgca gtaagcgcag ttgattttta tcggcgactt
    17041 tgagttgacc gtctttgtcg cgcaccagat tgatcgtctg gccattgacc acgatcgatc
    17101 cttcactcgg tttttcgagg aagttaatgc agcgcagaaa ggtacttttc cccgatcccg
    17161 acgatccgat gatgcttatt acatctccgg cattcgcttg cagtgagacc cctttcagca
    17221 cttcgtgttc gccatagcgt ttgtgcaaat cgataacgtt taatttattc tcggacatcg
    17281 tattctcagt gcgttgaaga aggtttcaca tgctgcaacc agcgtttttc cgctctgcga
    17341 aagaggctga tcaggacata agagatgatt aaatagagca ccgcggcaat gccgaaggcg
    17401 gtaaaaggtt gatacgtggc ggcgttaata tcgcgggcta ttttcagcag atccggcaca
    17461 gtggccgtaa acgccagcgc agtagagtgc agcatcagga tcacttcgtt gctgtatgcc
    17521 ggtaacgcaa tacgcagcgc agaaggcaaa ataatgcagc gatacatttt aaaggtcgag
    17581 aagccatagg ctctggcggc ttcaatttcc ccatgcggaa ccgaacggat tgccccagca
    17641 aaaatctcgg tagtgtaagc gcaggtgtta agcgtcagcg ccagcacggt acagttcagg
    17701 ccactgcgga agaaagcgtt aaggaattcg gttcccttaa caatctcaag cgtgtacatg
    17761 ccggaataga acaccagcaa ctgaacatac agcggcgtac cgcgaaaaat ataggtaaat
    17821 aaccagattg gaaactggat gtatttatta ctggagacac gaccaatcgc cagaaacagc
    17881 gccaggactc cgcctatcac taccgacaaa ataagcagcc acagagtgat cgccacacca
    17941 gtaaagcgat aaccgtcggt ccacagcagc ggtttccagt attcatgtaa gatttcgatc
    18001 acaggtcagc cctcttcaca cccacggagt agcggcgctc aaggaacagc agcacaccat
    18061 tggaaacggt ggtgaaaacc aggtaaatca cgccacagac gatggcgaaa tagaacggtt
    18121 cccaggtact tttgcctgcc agctgcgtgg ctttgaccac atcttccagg ccgagtaacg
    18181 aaaccagtgc ggtagatttg aggatcacct gccagttgtt gccaatgcct ggcagggcgt
    18241 aacgcatcat cgccggaaac atgatccgcc gaaacacttg cccgcgagta aaaccgaacg
    18301 ccgtcgccgc ctctatatgc cctttcggca ctgccataaa cgcgccacgg aacgtttcgg
    18361 taaaataagc accgtagata aaaccgagag tgataatacc agcgaccatc ggatcgatat
    18421 caatctgccc gacgcccatc gcctccgtca ccatgtttag cgcaatctgc aaaccataga
    18481 aaatcagcag catcaacacc aagtccggca cgcctcgaat cagcgtggtg tacccttcga
    18541 aaatcagccc cgaaagccga ttttgcgaga gcttaccgcc agcgccaatt aaaccgatga
    18601 ttacagcgag cactacagag ctgatagcca gctccagcgt gacgagcgca ccctgtaaaa
    18661 taacacctga aaacccatac aacatgctgc ctgtcctgtc gtgtgtggtg aattaccgcc
    18721 tcttgtctcc ctccggttac ccgaagggag cgggctttct ttcaccgatg gcgattaacc
    18781 accataaaca tcaaaatcga agtacttttt cgctaatttc tcgtaagtac cgtcagcgcg
    18841 catttcggca aaggctttgt tcagtgcctc gcgcagttcg ttatcttctt tacgcaggcc
    18901 catgccggtc cctacgccaa acagtttttc atctttaaca gacggaccac cgaatttgta
    18961 atctttaccg acgggttgtt tgaggaaacc ttcgctggca gcgacctcat cctggaacgc
    19021 ggcatcaata cgtccggcag tcaggtcaga ataaatgttg tcctgcccct gatacgagac
    19081 gatttcaatg ccttttggtg cccagtgttc attaccgaac gtctcctggg tggtgccctg
    19141 caatacgcct acccgtttgc ctttcagcga ctcgactgtc ggctgaatgt cagaattttt
    19201 cgccaccacc agacgagaat cggcagcgta cagtttgtcg gtgaaggcaa tttcttgctg
    19261 acgtttttcc gtaatggaaa gcgatgacat gatggcgtca atcttcttcg cttttaagga
    19321 cgggattaac gcatccagcg ggttttcgac aaacgtacat tgcgtattga tgcgcttgca
    19381 caactccttc gccagctcga tatcgaaacc aaccagttcg ccttgtgaat tttttgattc
    19441 aaatggcgca taggtcgggt cggtaccgat gcggatgttt tgcggaattg cagcaagtgc
    19501 cgcagttgcg ctggagaagg ccagaaccag agagagcgat agcaccagtt ttttcataat
    19561 gtcctcaact gacagtcttt ttatagggaa tatttacagg tttgttacac ttatcgtgcc
    19621 ataaaattga ccatcaaggc aaaatatccc gccccgttag acgtattatt tttcattaaa
    19681 aatgcttaag tatgcatttc aatgcaccac gatagtgcaa caaacctggc gtgcaccatg
    19741 atgatcattc catcaggtac agcttcccag cgacgtatca gtcaccgtag acattaaagt
    19801 cgaaatactt tttcgccatc ttgtcgtagg tgccgtcctg acgcagctcg ccaagcgcct
    19861 tattgaaggc agccgtcagt tcagcataat ctttacgtag ccctacaccg gtgccatcac
    19921 cgaagtattt tttgtctttt actgatgagc cagcaaaagc gaaatcttta ccagcaggtt
    19981 gcttgaggaa tccttcgctg gcagcaactt catcttgtaa cgcagcatcc agacgtcctg
    20041 cagccagatc ggaatagacc aaatcctggt tggcataggc caccacatcc acgcctttac
    20101 tacgccaggt ctcgttagcg taagcttcct gggttgatcc ctgcagcaca ccaacatgtt
    20161 tacctttcag tgaatccagc gttggctgaa tcggtgaacc tttggcagca atcaaacgag
    20221 aatctgcggc gtacagcttg tcggagaagg caatctcctg ctgacgttta tcggtaatgg
    20281 aaagcgacga aataatagcg tcgatttttt tcgctttcag tgaggggatc agcgcgtcaa
    20341 agtcactggc aacccaggta catttcacct gcatccgttt gcacatctcg ttaccgagat
    20401 cgatatcaaa gccaacaaaa tcacctttag catctttcga cgagaacagc gcataggtgg
    20461 tatcggttcc gatacgtacc gtctccggta gcgccgcata gctggaagcc gctgcggaga
    20521 gaccgactaa taaagacaga gcgagaatcg acttcttcat acataaccct caagtgaaaa
    20581 tggctttttt atgttgtgta ttgtgttgtg tgtttgcagg ctctttcatg caggtcttat
    20641 gccatcttga cgacaacagt aacattcaac gttaaatatg ttaataagac attgcattat
    20701 tgtcctgaag ttgaagatag caggtatagc ggttgaatcg cagcgtttcg attgaatggc
    20761 agaaacaaaa tgtcgaggat ttgatcgcgg ttgcaaaatt gccctgaaac agggcaacag
    20821 cggagttatg cgccctgcca gcgggcaaag agatcttcag gaagggttat tgcaaactgg
    20881 tcaagaacac gattaaccgt ctgatttatc acatcatcaa gggattgcgg gcgatgataa
    20941 aacgccggaa cgggaggcat aatcaccgca ccgatttctg ccgcctgagt cattaaacgc
    21001 agatggccta agtgcaatgg tgtttcacgc acgcagagca ccaacgggcg acgctctttc
    21061 agcaccacat ctgccgcacg ggtcagtaag ccatcagtat agctatggac aatgccggac
    21121 agggttttga ttgaacaggg taaaatcacc atccccagcg tctggaaaga accggaagag
    21181 atgctggcgg aaatatcgcg cgcatcgagc gtgacatcag ctaatgcctg cacttcgcgc
    21241 agggaaaaat ccgtttcgag ggataaggtc tggcgcgccg cctggctcat caccagatgc
    21301 gtttcgatat ctgtgacatc gcgcagaacc tgtaataagc gcacgccata aatcgcgccg
    21361 ctggcaccgc tgatgcctac aatgagtcgt ttcataaata gttgccctgt cagacttgca
    21421 ggcagacttt gcagtatttc ggcgggagtt gcaagtcagg gtgccagacc ggcaccctca
    21481 gcgaaggcat catccttcgt tatgcatttc gagattttcc acttcgttct gacgttgcac
    21541 tgctttggcg tcatcattac gtaacgtatc gaggaaatcg aggtagccct gatcaacatc
    21601 tttggtgacg tagacgccgt tgaacaccga gcattcaaac tgctggatat ccggattttc
    21661 agcgcgaacg gcgtcgatca gatcgttcag atcctggaaa atcaacccgt cagcaccgat
    21721 gatctggcga atttcatcaa cttcgcgacc gtgagcgatc agttccgtgg cgctcggcat
    21781 atcaatacca taaacgttcg ggaagcgaat ttccggtgcc gcagaagcga ggtacacttt
    21841 cttcgctccg gcttcgcgtg ccatctcgat aatctgctca gaagtggtgc cacggacgat
    21901 ggagtcgtcg accagcagga cgtttttatc gcggaactcg gcgcggttgg cattcagttt
    21961 acggcgcacg gacttacgac gcagctgctg gcccggcatg ataaaggtgc ggccaacata
    22021 gcggttttta acgaagccct ggcggtacgg tttgcccaga atacgagcaa tttccagcgc
    22081 gatatcacac gaggtttctg ggatcgggat caccacgtcg atatccagat cttcccattc
    22141 gcgggcaatt ttctcgccca gtttcgtgcc catattcaca cgcgcgctgt aaacggaaat
    22201 tttgtcgata aacgagtccg ggcgggcaaa gtatacatac tcaaacaggc acggattgct
    22261 gaccggattg tcagcacatt gacgggtaaa caactgccct tcttcagtga tgtaaatcgc
    22321 ttcgcccggc gcgacgtcac gcaggaaatc aaagcccagc gtatcgagcg ctacgctttc
    22381 ggaagcgacc atatattctg tacggttctc gtcaatatca cgttttccca gtaccagcgg
    22441 acgaatcccg tttggatcgc ggaaagcaac cataccgtgg ccgataatca tcgccacaca
    22501 ggcatacgcg ccgcggatta agcggtttgt ggcagcaatg gcagcgaaaa tattgtcggc
    22561 ttccagcggg tagtggcgga agttgtccag ctcgctggcg aagatattaa gcagaatttc
    22621 cgagtcggaa gtggtgttga tgtggcggcg tttttcttca aacagttttt tacgcaactc
    22681 gtgagcgttg gtcagattgc cgttgtgggc aagcgtaatg ccatacgggg agttaacgta
    22741 aaacggctgc gcttcagagg cgctggagct gccagccgta gggtaacgca catgaccaat
    22801 gcccatattg ccctgcaaac gctgcatatg gcgagcttca aatacatcgc tcaccagccc
    22861 gttcgcttta cgcaaacgga agcagttatt ggcatctatg gtgatgatgc cggcggcatc
    22921 ctgaccgcga tgctgaagca ccgttaaggc atcataaatc gactggttaa ccggcataac
    22981 accggcgata ccgacaatac cgcacatacg tctttttcct cgttaagcca catctcagag
    23041 cacttacgct ctgggcaaga aacttgacga gctttgcaga taatcaaaaa accatctgat
    23101 gataaaactg aattgcggga tcagctgtga tttgctccag tcttcacttt tcgacacccc
    23161 ggtaaaggag tcgagaaaga agagaatggc ggcaacaatc aacacaccgc gcaacgcacc
    23221 gaaacagacg cccagcaccc gatcggtgcc tgacaacccc gttttctcca ccaactggcc
    23281 tatcacgaag ttcacgatag caccaacgat cagggtagcg ataaacagta ccgcgatggc
    23341 aatcccattt cgaaccagtt cgtcttcaaa gcccgtaaac cagactgaca ggtaagtgta
    23401 gtaatgactg gcaacaaaga aagcacaacc ccatgtcacc agcgataacg cttcacgaac
    23461 aaagccgtgg atcaggctaa ccagagagga aaaagcaatc accgcgatta tggcgtaatc
    23521 aatccagacc atatgtgtcc cacgatttta cgccctgtca tcctgttcgg ggcgcattct
    23581 aacagaaaaa gaaaacgttt gcgtagggat ttccttcccg cgcatcaata aaaatggcgc
    23641 tgaaaaaata ttcaacgcca tcgacttttt atgcctttgc ggcatcgtgc aatgcgtgtc
    23701 ggatgcggcg taaacgcctt atccgaccta cggttctaac actgcccaag cctgataaga
    23761 cgcgccagcg tcgcatcagg caagactgta ttaattcggc gtatagccca ttaccacgcc
    23821 acttaagcca gaaagttgct tcaactcacc cagcgaacct ttcagcttat ctttcgaggc
    23881 atccggccca accagaatac gggtaatttt accctgcact ggcgtggatg gcgacgtata
    23941 aacccgataa ccggcaccgc gcagcttacc gacaatctca ttcactttat cggcattttt
    24001 cagcgcaccc agttgcacaa cataggcttt acccgttggc gctgcttttt cttccacgac
    24061 cggcttcggt tctggcttcg gcgcaggtgg tacttcaacc tttggtttag gcggctccac
    24121 cggtttcggc ttcggtgggg cgaccggtgc aggttccggt tcaaactcgg tgttattggc
    24181 tgcaatagtg gccggatcga gcgacggtgc tgcggcatca cctgcccgca cctcttccgc
    24241 tgcgccttcc ggcggctgcg tcggcaacgc ctgggtggcg gctggcatca tatcaggctc
    24301 atcacgatcg cccgctttcg gcaccagcgg gatagccgcg aactcatcct gataatgttt
    24361 tttctgcccg tccagcagcc ctggaagtac aatcaccccc agcgccacca gcacgatcgt
    24421 gcccactaac cgattctgaa acttacttgc caccgcttct cctcgcgtca atcacttcca
    24481 tgacatgtgc gaccgtgtgg aaagaaccac acaccagcac ggtgtcttcc gctttagcgt
    24541 ccgccattgc ggcatcccat gcctgcgcaa cgctatcaaa tgatttgccg ttacccaaat
    24601 gctcaagcag ttgttctgcc gtggcaccgc gcggcccttc cagtggcgca caataccagt
    24661 catcaaccac gcttttcaac caggccagag ttccggcaat atctttatca tgtagcatac
    24721 cgataaccgc cagcacgcgc ccgtttttcg gtagcgcttt catacgcccg gtgagatatt
    24781 ccgccgcatg tggattatgc gcgacatcaa aaataacgcg tggcgactcg ctcacaatct
    24841 ggaaacgtcc cggcaaaatt gcgctggcaa tcccgtcgcg aatggcattt tcactgactt
    24901 ccagcccgct ggcacgcagt gccgccagcg ccgttgcggc attcggttgc gggacaagcg
    24961 gcaacggcag attttccagc gtgccgtgag catcgctaaa cgcccagtca tgatcggtga
    25021 cggaatagtt ccactcaacg ccccgacgtt gtaacagtgc acctttttcc tgcgccacat
    25081 cagcaatggt agaaggcatt tccggctcac cgacaattgc cggtttttcg ctgcggaaga
    25141 tgcctgcttt ctcgagacca atactttcgc gatctggacc cagccagtcg gtatgatcca
    25201 gcgcaatact ggttactacc gcgacatcgg catcgacaat attggttgcg tccagacgac
    25261 cgcccagccc tacttccaga atcaccacgt caagttgtgc ctgcttgaac agccacaacg
    25321 ccgacagcgt accgtactcg aaataggtca gggaaatatc accgcgtgcc gattcaatct
    25381 ccgcaaaaga ggcggtgtgg gccgattccg gcaattcctg gccctgcaca cgtacgcgct
    25441 cggtataacg caccagatga ggcgaactgt agacgcccac tttgtaccct gccgccatca
    25501 gaatcgactc cagcgtacgg caggtggtgc ctttgccatt cgtacccgca acggtaaaca
    25561 caaatggcgc tggtttcagg acgccaagac gcgccgcgac ctggctcacg cgctcaaggc
    25621 cgagatcgat agttttactg tgcaggtttt ccagataaga aagccacgaa gccagaggcg
    25681 acgcggcttg aggagtgcgt ttgataatca tggtatccgc tgattcgtta cggtgagaat
    25741 agcaaaaggg cagagccagt ggccctgccc ttatcagtta tcaggcctca ggttcctgat
    25801 ccggtaccgg gggtaccact acgccttcac gcggcgcttc aggattcggc gctggcagat
    25861 tcatcaactt cgccagaatg ctcgccagtt tcaggcgcat ttccggacga cggacgatca
    25921 tgtcgatcgc gcctttctcg atcaggaatt cactgcgctg gaatctaggc ggcagttttt
    25981 cgcgaacggt ctgttcgata acacgcggac cggcaaagcc gattaacgct ttaggttcag
    26041 cgatgttgag atcgcccagc atggcgaaac ttgcagaaac accgcccatc gtcgggtcgg
    26101 tcagcacgga gatgtacggc aagccgcgct cctgcatttt tgccagtgcc gcagaggttt
    26161 tcgccatctg catcagcgac atcagtgctt cctgcatacg tgcgccacca gaggcggaga
    26221 agcagatcag cgggcagtta tcttcctgcg cctgctcaac ggcacgcacg aaacgtgcac
    26281 ccacaacaga ccccattgaa ccgcccataa aggcgaactc gaatgccgca gcgacaaccg
    26341 gcattccata cagagtgcct ttcatcacca ccagcgcatc tttttcgccg gtttctttct
    26401 gcgcagatgc cagacggtct ttatacttct tggagtcacg aaacttcagc tcatctttcg
    26461 gctcaagctc gctacccagc tccacaaggc ttccttcatc taacaggcta tgcaggcgat
    26521 tacgcgctgt catacgcatg tgatggtcac acttcggaca gacctcaaga ttacgttcca
    26581 gctcagcgcg gtataaaacc tgaccgcagc tatcacactt agtccacacc ccttcaggaa
    26641 tgctcgcctt gcgggtggga gtaatgttgc ttttaattcg ttcaatccag ctcattaggg
    26701 acctttctgt ctgaacctgg ttcgatgcca gttttatctt tggggacgca taatgccatt
    26761 tttgccccca acagaccatg aatgttgcac attaaaacat aacagcccga aactttggat
    26821 aaaaaagtgg tcgaaccgcg gagttacttt ttattttgcg gcgcgtgccg cagcgcgttt
    26881 gtgacggatt atttcgatga cgcccggcaa aatagaaacc acaataatcc cgacgatcag
    26941 cagcttaagg ttatcctgaa ccatcgggat tgtaccgaag aaatagcctg cgtaggtaaa
    27001 aagcagtacc cacaacagtg cgccgatcac gttataagca gcgaaatgac ggtacgacat
    27061 gtggcccatt cccgcaacaa acggggcgaa cgttctgacg atcggcacaa aacgggcgag
    27121 aataatcgtt ttgccgccat gtttctcata aaactgatgg gttttgtcga gataactgcg
    27181 acggaaaatt ttcgaattgg gattactgaa taacttttca ccgaacaacc gcccgatagt
    27241 gtaattgacc gcgtcaccca caatcgcggc gatcagcatc agcactacca tcatatggac
    27301 gttgagatcg ttggtttcca gtgatgccaa cgctccggca acaaacagca atgaatcacc
    27361 cggtaaaaac ggcattacca ccagaccggt ttcacagaac aaaattaaga acaaaatggc
    27421 ataaacccag acgccgtact ccgcgactag ctctgccaga tgcacgtcaa tgtgcaggat
    27481 aaaatcgatg aggaaataaa tcaggtccat atttgcctat gccttgtact cgtcatcttt
    27541 caggctgtaa ctacgttggc tacagcctga aatgttccga gcattattct cgttagtccg
    27601 ccagaaatag cgggcccatt ggcggttttg gaagatcata ccggtcaggg taatccaccg
    27661 cgaccagata cagcccttcc gcttttgccg ttgctgccgc cagcgttctg tcctttgccg
    27721 ccagcaactc tgctatccag ctctccggct ggttgtgggc accgacttcc atcaggctgc
    27781 cgacaatatt cctgaccata tgatgtacaa aggcattcgc tttgatatca accaccacat
    27841 aaggaccgtg acgcgtgaca ttaatatgca taacgttgcg ccacggggtt cgggactggc
    27901 actgcaccgc acggaacgag gtgaaatcat tctcgcccag caagcattgc gcagcccgat
    27961 gcatccgttc agcgtccagc ggttcgtaaa aatgggttac ccctttactc agtaccgccg
    28021 ggcgcagccg atgattgtag atgatgtagc gataacggcg agccgtggcg ctaaatcggg
    28081 catgaaaatc atcaggtaca gctttaaccc aacgcacggc gatgtcacca ggtaaattcg
    28141 catttacgcc caaggtccac gccgcgtctt tgcgctgcgc ggttgtttcg aaatgcacca
    28201 cttgcccggt accgtgtacc cctgcgtcag tacgcccggc gcagaagacg gtgatgggtt
    28261 cgttcgccac ctgggagagc gccttttcca gcttctcctg cacactgcgg acttcattct
    28321 gccgttgcca gccgtaatac ttactgccgt cgtactcaat gcccagcgca attttataaa
    28381 ctggcagttg ttgctggtcg gacattagta cagatactcc tgcaccagtt tctcggcgat
    28441 ttttactgcc atcagcgcgc cgccaaagcg aacgttatcg gccaccgacc agaactggac
    28501 ttgctccggc ataccgtagt cattacgcac gcagccaaca gaaagatgcg gcgtacccga
    28561 agcatcccct acctgagtcg ggaattcgtt ctcttcagag agcacaatat cttcgccctg
    28621 agcaaacgca tcacgcgctt cttctgccgc cagcggacgc agtgcttcaa agttgaccat
    28681 ctgggcatga ccgtagaata ccggtgcctg gacgacgctt gccgaaatca tcagcccttc
    28741 gtcctgcagg attttgcgta cttcgtcaac gatacgacgt tcttcacgca cgctaccttc
    28801 gctatccggc aacaacggca gcatattgaa cgccagttga cgaccgaaga aatcttcttc
    28861 gtcaatcgga atgccgttga gcaatttcgc actctgcccc gctaacgcat cgaccgcttt
    28921 tttgccctgg gcggaggctg aaatcaggct ggtaacactg atacgcgaca aaccgccctg
    28981 atcgattaac ggtttcagtg ccgccagcag ctggctggtc agactgtctg gtacggcgat
    29041 gacattccgg ttgcggtaat cggtcagtac aaacgggttt acttccggca ccaccagcgg
    29101 tacgtcgggt tcgagagcaa acagtccgct gctgtcgatc accaggcaac ctgagttggt
    29161 cgcttcttca acccaggcag cggtagcttc tttgcctgcg acaaaaaatg ccagctgcgc
    29221 ctgcgtccag tcgaattcag cggcatcctg cacggtgatt gttttaccac caaagcgcag
    29281 ttgttcgcct gcgctttcgt tacgtgccag tgcataaatt tccccaaccg ggaactgacg
    29341 ttcagccagc gtttcaagca gggcttcgcc catagcgcca gttgcgccca ggacggcaat
    29401 gttccagcct tcagacatgg tggtttactc cagaaatagc aaagctccct gccaatgtta
    29461 cagcagagag catgaagaag agattaacgt gccggatgat gaacggcgtt aaaacccagt
    29521 ttacacagca atgatgccgc actggcgtca tcacaaatta catacagaga tgaccattca
    29581 cggcgctcaa gatagttttt gcgcagttta tcgaactcac ccggtatccc ggcgacttta
    29641 cgcagcggtg cgtcatcgcg gcgcacatca tacaccaaat gcaccagcct tttcagcgtc
    29701 ggttgatcga gcggaccatg cagcgtaatg cggccaaact caggcgcggg cagcaacgta
    29761 tccagcgcaa cgtgttgttc atgcccaata aacttgctat aggcttcaaa cacttgcgtc
    29821 gtgccacgcg ctttaccctc cagggtatag cctgcgatat gcggcgtgcc gatatccact
    29881 tttgtcagca attccacatt aagttccggc tcgccttccc agacatccag caccacgctt
    29941 aacttctggc cttcattcag gcaggtcagc agcgcggtat tatcgacgac tgcgccacgg
    30001 caggcgttaa tcagaatcgc tccgggcttc aggctacgga tcagtttttc atccgccaga
    30061 tgtagcgttt tgtacggacc atctttaaag agtggcgtat ggaaagtcag aatatccgcg
    30121 cgctggacta actcatccag cgagcggaaa tcaccctcat ccccacggtc ggcgcgaggc
    30181 ggatcgcaaa gtaaggtttt aatccctaac gcttccagtc gcgcctgcaa tcgacgtcca
    30241 acgttaccaa cgcccacgat ccccaccgta cggtcgtgca gtgaaaatcc atcgcgttcg
    30301 gcaagcatca gcagggagga gaaaacatat tccaccaccg caatcgcatt acagccaggt
    30361 gcagcggaaa aaccaattcc cgcctgcttc aaccatgctt catcgacatg gtcggtcccc
    30421 gcagtggcag ttccaacaaa tttaatgggt tttcctgcca gcaaagattc attcactttc
    30481 gtgaccgaac gcaccatcag cgcatccgcg tctgccagtt gagcgacggg gattggacgc
    30541 ccgggaaccg cgatcacctc acccaaacgg ctaaataagt cgcgggcata aggcatattt
    30601 tcatcaacaa ggattttcac gtttgtgtta cctgtatgag acgagagtta accggacaag
    30661 tatgccataa tctcgcggcc aggcatactt gcgaagattt caggtataag gatacgtaat
    30721 gatacaacct atttccggcc ctcctcctgg gcagccacca ggtcagggag ataacctgcc
    30781 gtctggcgcg ggcaatcagc ctttatccag tcagcaacgt acttcgctgg aaagcttaat
    30841 gacgaaagtg acctcactga cgcaacaaca aagagcagaa ctgtgggcgg gtatcaggca
    30901 cgatattggt ctgtcgggag attcaccgct gctttcgcgt cacttccctg ccgctgagca
    30961 taatctggcg caacgtctgc tggccgcgca aaaaagccat tctgcccgcc agcttttagc
    31021 gcaattaggg gagtatttac gtctggggaa tcatcgtcag gcggtcacgg attatatccg
    31081 tcataacttt ggtcagacgc cgctgaatca gctctcaccg gagcaattaa aaaccattct
    31141 caccctgttg caggaaggga agatggttat tccgcaacca cagcagcgcg aggcgaccga
    31201 ccgtccttta ttaccggcgg agcacaatgc gctaaaacag ctggtgacca aacttgcggc
    31261 ggcaacgggg gaacccagca aacagatctg gcaatcgatg ctggaacttt ccggggtgaa
    31321 agatggcgag ttaattccag cgaaactgtt taaccatctg gtgacctggc tacaggcgcg
    31381 tcagacgcta agccagcaaa atacgccgac gctggaatca ctacagatgg cgctaaaaca
    31441 acctttagat gccagtgaac tggcggcgtt atcggcatat atccagcaaa aatatggcct
    31501 ttctgcgcaa tcatcgcttt cttctgccca ggccgaggat attcttaatc agctttatca
    31561 acggcgggtt aaagggattg atccgcgtga tatgcaaccg ctgcttaatc cttttccacc
    31621 gatgatggac acgttgcaaa atatggcaac gcgtcccgcg ctgtggatac tgttagtcgc
    31681 gattatcctg atgctggtct ggctggttcg ttaaccccga cgaaatgaca gtatcgtgac
    31741 aataataccc agcaccgcag agatcgcccc ggcaagaaat accgaagagt aaccaaacgt
    31801 ggtcgccagc attcccgcca gcggcccgga gacgccgagg gcgatatcct gaaacgcggc
    31861 gtaaccgccc agtgcggtgc cgcgaacttg tgaggggacg cgtttaacca cctccacgcc
    31921 cagcgcagga aagataagcg aacatccggc tccggttaac gccgcgcccg ctaatgcgac
    31981 ccatgcacct ggggcttgcc agagcagcaa caagcccacc gtttctacaa gcagagagac
    32041 aatcgccact ttcacgccgc caaaacggtc cggcatccag ccaaacatga cgcgcatcac
    32101 gacaaatgcg ccgccaaacg cggtaagagt aaagcccgcc atcgcccatc ctttgctggc
    32161 aaagtagagc gaaacgaaag tcccgataac cgcaaaacca acgccttgta gtgccagccc
    32221 taaccctggt ttccagataa gcccgacaac gctccacagc gatggacgtt ctcccgccag
    32281 ggccggtact ttgcgcactg tgccgttaca ggcccacgcc agtaagggta ataccattgt
    32341 ggtgatcgcc agtgcggcaa aaccgtaatg gctatgaatc aacaggccaa gcggcgcacc
    32401 aacagcgagg gcaccgtaaa tcgccattcc gttccatgac atcactttgc cagagtgttt
    32461 tggccctacg atgcctaacc cccaggtcag agcgcctgtc agtaactggc tttcaccaaa
    32521 cccaagaatc aaacgcccga tgaccaacag ggcaaatttg aacggtgcgg agacaggcaa
    32581 aatcgccgcc agcagcaacg cgccgccagc cagaccacaa gctaacatcc cctgaagcgc
    32641 cgaacgtttt gcgccatatt gatcggccag tcgcccggcg taaccacgcg tcagcaccgt
    32701 agccagaaac tgaatcccga cggcaatgcc gaccatggta ttgccatagc ccagatcatg
    32761 atgaacaaac agcgggataa ccggcaacgg caaccctacg gtcatgtagg tgagaaaaac
    32821 cgcaaaagag atgcggaaga gcgaaaaact ggcagaagat cgtgtttcgg tttggcttac
    32881 agcagtcatg cattactcca gaatgcagcg caaggcgagg agtatccccg tctcatctct
    32941 ctggtttcag ggttacggtg cgttggcagg atttaacgcg tacgtctttt cagaaggaaa
    33001 tcgacaaagc gggaagtttg cctagaactg gtggcgattg tcaatgatgt gaaaaaggga
    33061 accatcaggt tcccttttgc gttagtgccg gaggccgcat tggcgcgtaa cgtcggatgc
    33121 gacgctggcg cgtcttatcc gacctactcc ggcctactgc gaattaatct tttcagcttg
    33181 cgcattacca gcgtggcgtt ggtgccgccg aagccgaagc tgttagacat aacagtggtc
    33241 agttcgcgat cggtcgtttc ggtcacgatg ttcagacccg cagcctgctc gtccagctct
    33301 tcaatgttga tgctcggggc gataaagccg tgttccagca tcagcagaga gtagatagct
    33361 tcctgcacac ccgcagcgcc cagtgagtga ccggtcatgg ctttggttgc agaaatcgcc
    33421 gggctcttat cgccgaacac ttcacggata gctgccagct ctttcacgtc gccaaccgga
    33481 gtcgaagtac cgtgagagtt caggtaatcg attggggtat caacgccatg catcgccatc
    33541 tgcatacaac gtactgcgcc ttcgccagac ggagcaacca tgtctgcacc atcagaggtt
    33601 gcgccgtagc caacgatttc agcatagatg taagcaccac gcgccagcgc gtgttccagc
    33661 tcttcaacca ctaccatacc gccgccgcca gcgataacga aaccgtcacg gtgagcgtcg
    33721 taagtacggg aggctttttc cggggtgtcg ttgtatttag tagatagcgc acccatcgcg
    33781 tcgaattcgc aggccatttc ccagcacagc tcttcgccgc cgccagcaaa cacgatgtcc
    33841 tgtttgccca gttggatctg ctctactgcg ttaccgatac agtgtgcgga agtcgcacac
    33901 gcggagctga tggagtagtt aacgccatga attttaaacg gcgtggcgag acaggcagaa
    33961 acgccggatg ccatcgcttt agtgaccaca tacgggccaa ccgctttcag gccgcgcggg
    34021 ccgcgcattg cgtcagcgcc gaacacctgg aaacgcgggg aaccgccgcc ggaacctgca
    34081 atcaggccaa cgcgcgggtt attctggtaa gcttccggag agaggcccgc atcagcgatt
    34141 gcctgctcca tagaaaggaa tgcataaatg gatgcgtcgc tcataaagcg cacaactttg
    34201 cggtcaatga ggccagtggt atccagtttt atgttgcccc agacgtggct acgcatgccg
    34261 gaatccttca gctcctgaga gaaagtgatc cctgaacgtc cttcacgcag agatgccagg
    34321 acttcctgct ggttattacc gatgctggaa acaatgccca ggccagtaat cactgcacgt
    34381 ttcattcaat acctctgtaa gtcgcacata gagtaagttt cgaatgcaca atagcgtaca
    34441 cttgtacgcc gaacaagtcc gatcagccat ttaatagaga tatttgcgca gccttacaca
    34501 catcgctaag atcgagccac cgcctgtaag acgagtaact tacgtgaaac actactccat
    34561 acaacctgcc aacctcgaat ttaatgctga gggtacacct gtttcccgag attttgacga
    34621 tgtctatttt tccaacgata acgggctgga agagacgcgt tatgtttttc tgggaggtaa
    34681 ccaattagag gtacgctttc ctgagcatcc acatcctctg tttgtgatag cagagagcgg
    34741 cttcggcacc ggattaaact tcctgacgct atggaaggca tttgatcagt ttcgcgaagc
    34801 gcatccgcaa gcgcaattac aacgcttaca tttcattagt tttgagaaat ttcccctcac
    34861 ccgtgcggat ttagccttag cgcatcaaca ctggccggaa ctggctccgt gggcagaaca
    34921 acttcaggcg cagtggccaa tgcccttgcc cggttgccat cgtttattgc tcgatgaaag
    34981 ccgcgtgacg ctggatttat ggtttggcga tattaacgaa ctgaccagcc aactggacga
    35041 ttcgctaaat caaaaagtag atgcctggtt tctggacggc tttgcgccag ctaaaaaccc
    35101 ggatatgtgg acgcaaaatc tgtttaacgc catggcaagg ttggcgcgtc caggcggcac
    35161 gctggcgaca tttacgtctg ccggttttgt ccgccgcggt ttgcaggaag ccggattcac
    35221 gatgcaaaaa cgtaagggct ttgggcgcaa acgggaaatg ctttgtgggg tgatggaaca
    35281 gacattacca ctcccctgct ccacgccgtg gtttaatcgt acgggcagca gcaaacggga
    35341 agtagcgatt atcggcggtg gtattgccag cgcgttgttg tcgctggcgt tattacggcg
    35401 cggctggcag gtagcgcttt attgcgcgga tgaagccccc gcgctgggtg cttccggcaa
    35461 tcgccagggg gcgctgtatc cgttattaag caaacacgat gaggcgctaa accgcttttt
    35521 ctctaatgcg tttacttttg ctcgtcggtt ttacgaccaa ttacccgtta aatttgatca
    35581 tgactggtgc ggcgtcacgc agttaggctg ggatgagaaa agccagcata aaatcgcaca
    35641 gatgttgtca atggatttac ccgcagaact ggctgtagcc gttgaggcaa atgcggttga
    35701 acaaattacg ggcgttgcga caaattgcag cggcattact tatccgcaag gtggttggct
    35761 gtgcccagca gaactgaccc gtaatgtgct ggaactggcg caacagcagg gtttgcagat
    35821 ttattatcaa tatcagttac agaatttatc ccgtaaggat gactgttggt tgttgaattt
    35881 tgcaggagat cagcaagcaa cacacagcgt agtggtactg gcgaacgggc atcaaatcag
    35941 ccgtttcagc caaacgtcga ctctcccggt gtattcggtt gccgggcagg tcagccatat
    36001 tccgacaacg ccggaactgg cagagctgaa gcaggtgctg tgctatgacg gttatctcac
    36061 gccacaaaat ccggcgaatc aacatcattg tattggtgcc agttatcatc gcggcagcga
    36121 agatacggcg tacagtgagg acgatcagca gcagaatcgc cagcggttga ttgattgttt
    36181 cccgcaggca cagtgggcaa aagaggttga tgtcagtgat aaagaggcgc gctgcggtgt
    36241 gcgttgtgcc acccgcgatc atctgccaat ggtaggcaat gttcccgatt atgaggcaac
    36301 actcgtggaa tatgcgtcgt tggcggagca aaaagatgag gcggtaagcg cgccggtttt
    36361 tgacgatctc tttatgtttg cggctttagg ttctcgcggt ttgtgttctg ccccgctgtg
    36421 tgccgagatt ctggcggcgc agatgagcga cgaaccgatt ccgatggatg ccagtacgct
    36481 ggcggcgtta aacccgaatc ggttatgggt gcggaaattg ttgaagggta aagcggttaa
    36541 ggcggggtaa tctgctctcg cagtgtttgc cggatgcggc gtgaacgcct tatccaacct
    36601 acgtgtgacg tgtaggcctg ataagacgcg ataagcgtcg catcaggcat ggtgctccag
    36661 acgccgcaac ttacagctgt gacgcctgct gaaacaggtt ttcccacata tcggtcacca
    36721 gcgcctggtc acgcggcgac aattcaccgg caccaatggc tttttccaga ctctggctaa
    36781 cggtcgtatg caccgcctga gcggagtggt catcaccact ttccagttct gcgatggcta
    36841 atgtcaggtg gccacgcaaa tacccactgg caaacaactc atcatcactg gcatggtcaa
    36901 ccatgccgtc gattaatgcc agaatgcgtg attcaaactc cgcgatcatc ttctttcctc
    36961 attgtaaaac gtggcgctgg cttcagttga gcgcttccgg ccacgggaac tgttccgccg
    37021 taagttccgg cgtgtgataa taattttgta gtgctttaat gaagcgtgcc gggcgttccg
    37081 gaatgccatt tgtcagataa tccatcacct gcgcatgtac ccgccgttga aacaccacac
    37141 gatccggttc gaaatcccct tccagattgt cgcaactgac gttaaacgga tatccagccg
    37201 ccacgcagaa taaccagtcg aacgcctgcg gcttcacttc aacatcttca aactggcttt
    37261 gcgtctgggc atcgcgtccg tccgggcaat accagtagcc gaaatcaacc agctcacggc
    37321 gcgcttttcc ggcaatacac cagtgcgaaa tctcatgaat tgcgctggca taaaagccat
    37381 gagcaaagac gattcggtta tacggtactt ccgcatcagc aggaagatag atcggttcgt
    37441 cgtcgccttt aatcagacgg gtattaaatt catcggcaaa gcagctatta aaaatttcaa
    37501 tcaactgctc gtagtggtgt gtactgttca ttagttcatc cccaaccagt ggaggatctc
    37561 ctgtccgtgg ctatcgtaaa gtaatttggc actcatcacc gccgagacga taacaatcat
    37621 cgggcggatc agcttttgtc ctttgctcaa caccaaccgt gaacccatgc gcgcgccgag
    37681 gaactgcccc accagcatga caaagcccgt cgcccaaatc actttgccgc cgagaataaa
    37741 cagcagcaga ccgccaatgt ttgacgttgc gttgagtaat ttggcatgag ccgtggcttt
    37801 ggcgaggttg aatccgcaca gcataacgaa ggccagcgcg taaaacgatc cggcagccgg
    37861 gccaaagaat ccgtcataaa aaccgacgca gccaccggca attaacgcga agggtaaacc
    37921 gtacatccgg cgctggcgat cttcttcacc cagctttggc atcagcaaga aatagaggcc
    37981 gatacaaatc accagaatgg gcaaaatctg ccgtaagaca tcagcctgaa cgtactgtac
    38041 caacagtgcg ccgctcattg agccgacaaa ggtcatggca atattgagtt tctgatcgct
    38101 taaactaacc actttgcggc gaataaagta gatagtggcg gaaatagagc cgccgcaggc
    38161 ttgcagttta ttggttgcca gcgcgttagc gggagacatt cccgcagcca tcaatgccgg
    38221 aatggtgagt aacccaccgc caccggcaat cgagtcgata aatcccgcca gcatggcgac
    38281 aaaaaagaga actcccagca acagcggaga aaccataaac aggctattaa acgtttccat
    38341 tagatcacgt gctcatccag tagcgcctgg caggaaggcg gcaacggagg cggtgtcttc
    38401 ttctcaggct ttgttgttcc cggttttgga ggttcaaacc agctttgcag ttctgccccg
    38461 caaccatcgc ctgatggcgg taaaggttga tcttcacact ccagactatc ggcagggcaa
    38521 cgtaatcgta catgcatatg cgcgcgatgc tggaaccagg gtcgcacttt gcgcaaccag
    38581 tcgcgatcgg tgcccgcatc aaggcaaagt tgttgtttaa tcgccggatt aacaaaaatg
    38641 cgcgtgacgt ctttgtcctg ggcggcgagt ttgatcaagc tgaaaatttc tggcttccac
    38701 agcgtggaga caacgtgttt accgtcgcgg gaaactaagt ccagtgcttg cgggcgcaag
    38761 agctgcgcgg aggtccagcg agttttcggc agttgcagaa agatatcgac atccagtccg
    38821 gtctggtggc tggcatgacc gccgttgaaa cgcccaccag cgggcatccc catatcgcca
    38881 atcagcaccg tacccatgcc cagattgctc acctggctac tcagacgctg gataaacatc
    38941 accagatccg ggtgaccgaa atagcgacgc tgatcggtac gcatgacctg ataatgttcg
    39001 gactgtatcg gcaacgtgtc agcgccgaca atacagccat tagaaaaact gcctatcgat
    39061 tgtgcgctac ccggcacagg ttgggttatt ttttgccacg gcgtcgctgc caggctagca
    39121 ctactggcaa gcagagccag cagcgcaatc gcggttttgt tcatttttta ccagcgtgga
    39181 atatcagtct tcacatcggc attttgcgcc cgctgccgta acaggtgatc cattaaaacg
    39241 atcgccagca tcgcttcagc gatcggcact gcacggatcc cgacacaggg atcgtgacgg
    39301 cctttggtga tcatctcaac ttcttcgcca aagcggttaa tagtgcgccc cggcacggta
    39361 atgctggagg tcggtttcag cgccatatga gcaatgattt gctgcccgct gctgataccg
    39421 ccgaaaatgc cgcccgcatg gttgctctgg aaaccgtctt tggtgatttc gtcgcggttc
    39481 tggctgccgc gcagcgccac tacgtcaaaa ccgtcgccaa tttccacgcc tttcaccgcg
    39541 ttgatgctca tcagcgcatg ggcgatgtcg gcatccaggc ggtcaaagac cggctcgcca
    39601 agtccggcgg ggacaccact ggcaacaacg gtgactttcg cgccgatgga gtcgccctct
    39661 tttttcagcg cgcgcatcag ttcatctaac gcgtcgattt tgtcggggtc cgggcagaag
    39721 aacggatttt gttcaaccag cgaccagtct ttgatttcca gcggaatgtc gcccatctgg
    39781 gtcaggcagc cgcggatttc aataccaaat ttctcggcga gatatttttt ggcaatcgcc
    39841 cctgccgcca cgcgcatggc ggtttcgcgg gcggaagaac gtccaccgcc gcgataatcg
    39901 cgcagaccgt atttttgttc gtaggtgtaa tcagcatggc ctggacggaa gacgtcttta
    39961 attgcgctgt aatcctgaga acgctggtcg gtattttcga tcaacaagcc aatgctggtg
    40021 ccagtagtaa cgccttcaaa aacaccggag agtattttga cctgatccgg ctcgcggcgc
    40081 tgggtggtat agcgcgatgt cccagggcga cgacgatcaa ggtcatgttg caggtccgct
    40141 tccgtcagcg gaatgcctgg cggaacacca tcgacgatgc agccgagcgc cagcccgtgc
    40201 gattcgccga aggtggttac gcgaaagagt tgtccaattg tgtttccagc catcacggct
    40261 ccgttattgt tgtgtttgcg tgtttactta atctttataa atcgcgaaat gttctcgtgc
    40321 ggcaataagc tgctctttag tgagcataaa cacaccatcg ccgccgttat caaactccag
    40381 ccaggtgaac ggaacatcgg gatattgttc cataagatgt accatgctgt tgcccacttc
    40441 acaaatcaac acgccatcat cagcaaggta atctgccgcg ttaccgagaa tgcgacgcgt
    40501 cagtttcagg ccgtcggtgc cagatgccag gcccagttcc ggctcgtgac ggtattcgtt
    40561 tggcaggtcg gacatatctt ccgcgtcgac atacggcggg ttagtgacaa tcagatcgta
    40621 ttgcactttc ggcaaatcac ggaacagatc ggaacgaatc ggaatgacgt tgtggatcag
    40681 accgtgttct tcgatgttct gttcggcaac cgccagcgcg tcgggagaga tatctaccgc
    40741 gtcgacttcc gcttccggga aggcataagc acaggcaatg gcgatgcagc cgctaccggt
    40801 acacatatct aaaatatgct gcggttgctt gctgatgagt ccggcaaatt tattgttgat
    40861 cagttcacca atcggcgagc gcggcaccag cacgcgttca tcgacgtaaa attcatgacc
    40921 gcagaaccag gctttgttgg tcaggtaagc caccggaatg cgctcgttga cgcggcggat
    40981 cacgcgttca acaatacggt gtttttcgct ggaggtcaga cgcgcggtgc gcatatcttc
    41041 cgggatatcc agcggcaggt agagcgatgg taacaccagc tgtacggctt catcccacgg
    41101 gttatcggtg ccgtgaccgt accagatatt tgccgcgctg aagcggctta ccgaccagcg
    41161 caatatgtcc tgaatggttt gcagctcatt tactgcttca tcaacgaaaa ttttatccac
    41221 gtattcctcc agggcatgat cgcaataatt tcggcggcta gtttgccatg aagatgacga
    41281 taaatcagca ttcacgcgcg gtgagtgagg aaaaatacgt ttaaaacgat cgattgtgct
    41341 acgagtcggg taaactgtag gaaaattaga aatagagaca gataaatgaa aaagaaaaca
    41401 acacttagcg aggaggacca ggctctgttt cgccagttga tggcggggac tcgcaagatt
    41461 aagcaggaca cgattgtcca ccgaccgcag cgtaaaaaaa tcagcgaagt gccggtgaaa
    41521 cgcttgatcc aggagcaggc tgatgccagc cattatttct ccgatgagtt tcagccgtta
    41581 ttaaataccg aaggtccggt gaaatatgtt cgcccggatg tcagccattt tgaggcgaag
    41641 aaactgcgcc gcggcgatta ttcaccggaa ttgtttttgg atttacacgg tctgacgcag
    41701 ctgcaggcca agcaggaact gggcgcgttg attgccgcct gtcgccgcga acatgtgttt
    41761 tgtgcctgcg tgatgcatgg gcacgggaag catattttga agcaacaaac accgctgtgg
    41821 ctggcacaac atccgcatgt gatggcattt caccaggcac cgaaagagta cggcggtgat
    41881 gcggctttgt tggtgttgat tgaagtggaa gagtggttgc cgccggagtt gccctgaatg
    41941 ctggcagcaa atgccggagc agcgtaaacg ccttatccgg cctacaaata tgtgataatt
    42001 caataaattg caattcactt cgtaggcctg ataagacgca tcagagcaat actacgaact
    42061 tcagttcatt caatacatac tgaaaacgac acgtcgaatc aatcggttgc ccactactgg
    42121 tattcacatt caccacaata tcgtgtagta ctttatccgt taccggatga cgagttggcg
    42181 taaccgttac cgttgattta tcacaaccgt aagaggtaaa ggttaacaat tctgataccg
    42241 cgcggcagta tgctcctctg ccatcattat cagaaaaatt actgccatca cagagtctgc
    42301 cgccttccat caggtaggtc gaaagcgtgg tggtcactgt tgaccccgtg gactgaattc
    42361 gtaacgcctt attatggctg acataatttt cactccagga gatatcgtat gaagcgaccg
    42421 gtatcgttgg gcatattgtg ttatgcactg taggggccgt atagcttcca aatccgggta
    42481 aatctgtata ggaataaaag gatgcgatcc aagggcaacc ataatcatca gatgcaacgg
    42541 taattttaaa cgtcatatat gcacaacgaa aaattttcgt atctatgcca gggcaatttg
    42601 agttattaat tgtatcaaag tcagtcagta ccaaagttgg agctgaaccc gacccagacc
    42661 aggaaataac ccgcaacgta tcgcctttat acgcaaaaga atcatatttc ccatatgcaa
    42721 cctcctttgg cgttatcacc gtaacattga aaacactgct cgctacagca ccggttacct
    42781 tgtccatggc ataataaact ctcacactgt caaaactcgc actcataagt ttatctccag
    42841 cataactctt agatgcgccg acaacactca gaacgagcag cattaggctc gcgaaaatta
    42901 tggtccattt tttcatttca gaaatactca aaggttgccg tcactgtggc actaaagtct
    42961 ccggacgtta catctcgccc gctctttgcc tgtaaccagg tattaaaatt taggctgtta
    43021 tttccctgat tcagcgggag cgtgacgcct gttgtattgt tgattggcac cgatgtgcct
    43081 gccgcagttt caataccaat cccagcgccg gatgctgatg acgaggagtc aaaggccaga
    43141 aagccgggta gagcactatc ttctgttcct gtcaacgtta ctttgacatt aaacaatgta
    43201 gaactatgac aatctttcaa ttgaaacacc aatggcactc gctctgactg cccaaggttc
    43261 attaaatcac tggcggcaat ggcaggaaaa tgtacttctg ccaactggct accattaatc
    43321 actggcgtac acgatttact gattaaattg ccggtaaagt gcaaattatc tcctgctatt
    43381 gtggttccac tgcccagcaa catcaggcta ctaataaaaa acaacgtacg ctttttcttc
    43441 attggtaatc tacctgtaac gtggcccagg cctcaaagtc cgcctctggc agaatgacac
    43501 cactcttttt tactggcaca gccgttaaca ccggtaaatc ggtttcattc accacaagtg
    43561 gcgtattgat cgtaaaactc tgccctgcct gtttgagctg aatgcctaaa ccagtgatat
    43621 tgctgcttac tgcagcatta tcaaaatcgc ttggtgtgcc actccagctc aaggtcattt
    43681 ccattaatgg gtcgcgaacc gccgtatcgc aggtaatgga atacggcacg gtttgctcat
    43741 aattgctgcc attaatgtca tcaataagca catcctgaaa agttatttca atggtttgag
    43801 cgttgctaat cgtgcagtta ggcggctgca ctaaataacc gtgaaaagag acgtttgaga
    43861 gtgccatcga gcaaaagccc cacagcaaaa taagcgagat ccgtttcatt ggtaatccac
    43921 cacaattgtg gcactggcgt taaattcagc tgccgctaac gttgtaccac tctgtttcac
    43981 cggcacagct tccagaacgg gaactcccga ggagaaatta aatggcaacc atgagccaga
    44041 ggttaagtcg agtattgtgt gatcactact tttctgaacc cgaataccaa acccaggaat
    44101 acccgtgctg ataactgttt caccgttaat cacgaccgtt gtcgcctgca tctgtagacg
    44161 caggtcatcc attgccagat tgttacaaac aagcgagtag tcaatgggac gtttataatc
    44221 aaccctattg atcttattaa tgaatacatt accaaactca actgccgcac catttaccgt
    44281 acaaggagga ggggcatcga caagcacccg taaattcata cttactgact tactcgctgc
    44341 aactgcaaag ccgctaccaa cacaacatat cagtaaccca gttaccgccc agacagaaca
    44401 agatcctttc atactggctc tcccggcctc agcctttgcg gctctgctct tcatcgacct
    44461 agcaactgtt accgttgcaa gtgaaaaaga gcggcatccg agcgccatag tcgttgacat
    44521 aggtcagtac cggcacgcta cccatcttca cattcagcgg tgcagaggtt ttcggcgaca
    44581 tcactattgg cgagaaaccg gctgccggat tgccattttt ctgtgtgctg gcattactga
    44641 aaatgacgta atacgacgtc gggttattga cggtaaattc ctgaccgttg cgggttaaag
    44701 taatcttata ttgctagggg tttttcatgc tgacgttctc cagcgccttc ggtcgccaga
    44761 acaacttaat acgcgtttgt agtgctattt gcagcgtgtt ggctttgttt gattttggcg
    44821 ggatctcacg cacgttgaag tagaacaggc tttcacgatc ggcaggcaat ttgttgatat
    44881 ccggcattcc ctgcactttt acctgcccgt tcatcattga atcgatgcgt tgcaccggtg
    44941 gcagaacagt taacggtgaa ctgatcttgt tacccttttc atcttcgatc cagctttggg
    45001 ccagataagg cagtttcgga tcgttattgc gcaacgtcac gctgattgat ttgtcgcttt
    45061 cattaaatat caaacgggta cgatctggcg ttacagaagc ctgggcagac aaactggcag
    45121 cgctaagcag taacgctagc gtcgtagcac ccaatttggc agagcaaagt aaatcagaca
    45181 tggatcattc cttaatcacg ttattggtta gctgaaacag aaacaggggg attggtgggt
    45241 gtcacctgtt gcgtctgctc ctgaatgaca ggctttatgt catcaggaac aaccggcgcg
    45301 acgttgcctt tatgctggca tggcagtaac aagccgttaa acagatcggc aggcagcggg
    45361 tccggcaggt tgatatcgca atgcgcaaca ccactccaga acacactcat gtgttcgcca
    45421 ggtttcactc cagccagata aacattgccg tcatcatcga caaggcccac tgtctgctcg
    45481 ttatcatttt ttacttctgc gccaaacgga ggatggctgc cgtcttgtaa acgcagcacc
    45541 gccatcgctt tttgaccact gatgacggca aatttgcggt agccaatcgc cccttcagtt
    45601 agcgtggctt gtaccaccga ctgggttgct tcggcgtttt ccggcagttt gttgaggtcg
    45661 atatacgcct gattgcggta atagttattg acgtcagaaa cgacggcttt accaaacata
    45721 ttggtataaa cagcagcacc gttaccttct accggaacat cggcaacgcc atcggcatca
    45781 atcaacaagc gtgtcccgcc catattctgg gtacggtgaa gtgcgccacc gtgggcagta
    45841 agcgttgcgc cgccctgtaa cgacaagccc gcagaggtgt attgcccttc atggtagttc
    45901 gcactgaggt caacctgcgc cagcgaacca tcatggctgt aatagccgtc aacgctggtg
    45961 tgtttgtcac tggtgccgac gttcaactga tagtgagtcg cgtcatcgac acggctgaaa
    46021 taaccgacct gactgctgtc cgtcccactg ccatagtacc gttatagcta acggtactgt
    46081 tgtcgcccca cggcatactg agcgaaatgt acatgccttt gtcggcctgg ttgtcatact
    46141 cgtaacggta gccagtcatc gagatgctga cattacgaat actgcccata ttgaaatagt
    46201 gcgagagcat gatgttgtag tttgtctgct cctcgcgatc ccagtaggta tggcgggtat
    46261 agttgagata aaccgaaaca cccgcatcgc ggaagttctg gttataagtg gcggtgtaca
    46321 tctctttgtc gttgcccgtg cgtaccattc cgctgtcact ggcatccaga tactcgctca
    46381 tggtcataaa gttctcttcc gagaagcgat atccagcaaa agtaacgcgg ctgttgagct
    46441 ggtcaaagtc tttggaataa ctcacacgga aggagttacc gtccagcgaa cctttgccat
    46501 aagcggtgtc tttatccagt ttggtgtgcg agtgagtaac atcaaacgcg accgcgccga
    46561 atgtagacaa atcgcgaccg acaccaagcg ccgcagactg atagttttca tctcccagtg
    46621 cgccgccata taacgaccag ccgttagcga tcccccagga agcttcagca ccagaaaaaa
    46681 attcaccctc gacatggtgt ccccactctt gcggacggcc catcatgatc ttatagcgaa
    46741 cctgacctgg acgagtgagg tatggcatcg aggcggtgct gatgtcatat tcctgcacct
    46801 ggccgttctg ttcttcaatg cgaatatgca acgtaccgga gacagaatca ccaagatcct
    46861 gaatacgaaa cggtccagcc ggaacctgcg tttcgtaaat cacacgcccc atctggctga
    46921 cggtcacttt tgctgtggtg tgtgccacgc cggaaatgtc tggcgcgtag ccgcgcagat
    46981 tgggaggcaa catttgatcg tcagtactga cactgccacc aacatagtta aaaccatcaa
    47041 aaatatcgga tctgaggtaa tcctcgccca gcgccagttt ggcttttaat gatggtaacg
    47101 cccgcctggc atagtagcga ctccactccc atttttttga gtttcatcgc cgctgaattc
    47161 atcgtcatca ttactgcgag tatgttgata gttagtctgc cagtcagcac gcatacgcca
    47221 cggccctagg ttaaccccaa ccgtcccgtt gccgctgatc tcgttactat catcaccgcc
    47281 attttcttcg tgccgggttt gtgcgttgat gctgtagtcc gcgacgatcc cggagatgcc
    47341 gtcatcccaa cgtgaaggcg gatccctgtc gggataagta tattcgaggt aagcctgtgg
    47401 cagtgaaatg actaatgcgg actggcttaa atcagcctta atttccatgc cttccagttg
    47461 accggatttc aggcatttag catcgtggct ccattgcaga tttttcgcca cgtcttcttt
    47521 taaaccaaac tgcgctacca gttccggtgt cagacaagca tagcttttgc tcgcgtcatc
    47581 ttcaccagca taccagtaaa tatcgtactc ttccgccaat ggctgtttat ttagttgaac
    47641 ctgtaaattg tattttccgg gctcaacata tccctggctg gaaaaacgct tcatatcaat
    47701 ttttgtgtcg ccttttaatt ccagaaaacg ggaatcaaac tgaatgtcgt cttcagccca
    47761 gacactgcta taactccctg acattgccag cgcaatgcac cacggaagaa tccgtaatcg
    47821 aaaaagagaa tggtcaggca tacacgtttc tatccgtttt tattcttgtt cggtcaaaat
    47881 acatttaaat cccccatagc agtgtcgcta tgggggtatg ccttacaggt aagtaatctg
    47941 gaaggtggta tttgcttcaa aattacccag atctggcgca tcagctgcgc cgaccagcca
    48001 ggcgttaaag ttcagtgtct gcttcgcttt gcctttgttg gtagaagtgt ctttcactat
    48061 tttctggtca atacccatgc cgcttttgta agaggtaccc agagagtcac cgatcgccag
    48121 gctaacattg ttaaatgccg caccggtatc ggtattgaaa atggtgtaat aattgccgct
    48181 atttgcagaa gaaacggtac cagtaaaggt agtggtcatt gtttcctgag tatcaaatac
    48241 gcaatcttgc agacgaatct ggaaactttt cggcgtggtg gtgccgttat ttttcagttt
    48301 ttcggcaccg atatcaccca tatcaacttc cagtttgtga tcgtccggaa cgatagaaca
    48361 cactgagtct tcaatggtgc cgtaaaaacg tgcagtaccg ttgttacccg cagcgattgt
    48421 tgccgtagag gtgaacacg
//
LOCUS       DAZSIO010000011        48023 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZSIO010000011 DAZSIO010000000
VERSION     DAZSIO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48023
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.011"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(95..3244)
                     /gene="acrB"
                     /locus_tag="ACHX5T_000535"
     CDS             complement(95..3244)
                     /gene="acrB"
                     /locus_tag="ACHX5T_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR8465866.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3267..4460)
                     /gene="acrA"
                     /locus_tag="ACHX5T_000536"
     CDS             complement(3267..4460)
                     /gene="acrA"
                     /locus_tag="ACHX5T_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR8465867.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4602..5249
                     /gene="acrR"
                     /locus_tag="ACHX5T_000537"
     CDS             4602..5249
                     /gene="acrR"
                     /locus_tag="ACHX5T_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR8465868.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5377..8739
                     /gene="mscK"
                     /locus_tag="ACHX5T_000538"
     CDS             5377..8739
                     /gene="mscK"
                     /locus_tag="ACHX5T_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR8465869.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8951..9112)
                     /gene="rsmS"
                     /locus_tag="ACHX5T_000539"
     CDS             complement(8951..9112)
                     /gene="rsmS"
                     /locus_tag="ACHX5T_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR8465870.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(9126..9653)
                     /gene="priC"
                     /locus_tag="ACHX5T_000540"
     CDS             complement(9126..9653)
                     /gene="priC"
                     /locus_tag="ACHX5T_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR8465871.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9723..10100
                     /locus_tag="ACHX5T_000541"
     CDS             9723..10100
                     /locus_tag="ACHX5T_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR8465872.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10253..10804
                     /gene="apt"
                     /locus_tag="ACHX5T_000542"
     CDS             10253..10804
                     /gene="apt"
                     /locus_tag="ACHX5T_000542"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR8465873.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10933..12858
                     /gene="dnaX"
                     /locus_tag="ACHX5T_000543"
     CDS             10933..12858
                     /gene="dnaX"
                     /locus_tag="ACHX5T_000543"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR8465874.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    12191..12255
                     /gene="dnaX"
                     /locus_tag="ACHX5T_000543"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12911..13240
                     /locus_tag="ACHX5T_000544"
     CDS             12911..13240
                     /locus_tag="ACHX5T_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR8465875.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13240..13845
                     /gene="recR"
                     /locus_tag="ACHX5T_000545"
     CDS             13240..13845
                     /gene="recR"
                     /locus_tag="ACHX5T_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR8465876.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13955..15829
                     /gene="htpG"
                     /locus_tag="ACHX5T_000546"
     CDS             13955..15829
                     /gene="htpG"
                     /locus_tag="ACHX5T_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR8465877.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16010..16654
                     /gene="adk"
                     /locus_tag="ACHX5T_000547"
     CDS             16010..16654
                     /gene="adk"
                     /locus_tag="ACHX5T_000547"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR8465878.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            16786..17748
                     /gene="hemH"
                     /locus_tag="ACHX5T_000548"
     CDS             16786..17748
                     /gene="hemH"
                     /locus_tag="ACHX5T_000548"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR8465879.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17745..18704)
                     /gene="aes"
                     /locus_tag="ACHX5T_000549"
                     /pseudo
     CDS             complement(17745..18704)
                     /gene="aes"
                     /locus_tag="ACHX5T_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            18856..20160
                     /gene="gsk"
                     /locus_tag="ACHX5T_000550"
     CDS             18856..20160
                     /gene="gsk"
                     /locus_tag="ACHX5T_000550"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR8465880.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(20293..21969)
                     /gene="ybaL"
                     /locus_tag="ACHX5T_000551"
     CDS             complement(20293..21969)
                     /gene="ybaL"
                     /locus_tag="ACHX5T_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR8465881.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22207..23427)
                     /gene="fsr"
                     /locus_tag="ACHX5T_000552"
     CDS             complement(22207..23427)
                     /gene="fsr"
                     /locus_tag="ACHX5T_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR8465882.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23645..25297
                     /gene="ushA"
                     /locus_tag="ACHX5T_000553"
     CDS             23645..25297
                     /gene="ushA"
                     /locus_tag="ACHX5T_000553"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR8465883.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25334..25813)
                     /gene="ybaK"
                     /locus_tag="ACHX5T_000554"
     CDS             complement(25334..25813)
                     /gene="ybaK"
                     /locus_tag="ACHX5T_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR8465884.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(26017..26811)
                     /locus_tag="ACHX5T_000555"
     CDS             complement(26017..26811)
                     /locus_tag="ACHX5T_000555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR8465885.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            26949..27290
                     /locus_tag="ACHX5T_000556"
     CDS             26949..27290
                     /locus_tag="ACHX5T_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR8465886.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27505..30009)
                     /gene="copA"
                     /locus_tag="ACHX5T_000557"
     CDS             complement(27505..30009)
                     /gene="copA"
                     /locus_tag="ACHX5T_000557"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR8465887.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30271..31203
                     /gene="glsA"
                     /locus_tag="ACHX5T_000558"
     CDS             30271..31203
                     /gene="glsA"
                     /locus_tag="ACHX5T_000558"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR8465888.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31206..32498
                     /locus_tag="ACHX5T_000559"
     CDS             31206..32498
                     /locus_tag="ACHX5T_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR8465889.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            32529..32626
                     /locus_tag="ACHX5T_000560"
                     /pseudo
     CDS             32529..32626
                     /locus_tag="ACHX5T_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32623..33030
                     /gene="cueR"
                     /locus_tag="ACHX5T_000561"
     CDS             32623..33030
                     /gene="cueR"
                     /locus_tag="ACHX5T_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR8465890.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(33031..33489)
                     /locus_tag="ACHX5T_000562"
     CDS             complement(33031..33489)
                     /locus_tag="ACHX5T_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR8465891.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(33486..34403)
                     /locus_tag="ACHX5T_000563"
     CDS             complement(33486..34403)
                     /locus_tag="ACHX5T_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR8465892.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            34549..35226
                     /gene="fetA"
                     /locus_tag="ACHX5T_000564"
     CDS             34549..35226
                     /gene="fetA"
                     /locus_tag="ACHX5T_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR8465893.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            35213..35992
                     /gene="fetB"
                     /locus_tag="ACHX5T_000565"
     CDS             35213..35992
                     /gene="fetB"
                     /locus_tag="ACHX5T_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR8465894.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(36055..36909)
                     /gene="cnoX"
                     /locus_tag="ACHX5T_000566"
     CDS             complement(36055..36909)
                     /gene="cnoX"
                     /locus_tag="ACHX5T_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR8465895.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(36970..37779)
                     /gene="ybbO"
                     /locus_tag="ACHX5T_000567"
     CDS             complement(36970..37779)
                     /gene="ybbO"
                     /locus_tag="ACHX5T_000567"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR8465896.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(37769..38391)
                     /gene="tesA"
                     /locus_tag="ACHX5T_000568"
                     /pseudo
     CDS             complement(37769..38391)
                     /gene="tesA"
                     /locus_tag="ACHX5T_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            38362..39048
                     /gene="ybbA"
                     /locus_tag="ACHX5T_000569"
     CDS             38362..39048
                     /gene="ybbA"
                     /locus_tag="ACHX5T_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR8465897.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQLFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            39045..41460
                     /gene="ybbP"
                     /locus_tag="ACHX5T_000570"
                     /pseudo
     CDS             39045..41460
                     /gene="ybbP"
                     /locus_tag="ACHX5T_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
     gene            complement(41678..41833)
                     /gene="dinQ"
                     /locus_tag="ACHX5T_000571"
     CDS             complement(41678..41833)
                     /gene="dinQ"
                     /locus_tag="ACHX5T_000571"
                     /inference="COORDINATES: protein motif:HMM:NF041472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR8465898.1"
                     /translation="MKKIIVIGNVRVSCDCLSPLREQRMKTFLDKVLIVLKALIALLE
                     LIRQFIE"
     gene            complement(42173..43267)
                     /gene="mnmH"
                     /locus_tag="ACHX5T_000572"
     CDS             complement(42173..43267)
                     /gene="mnmH"
                     /locus_tag="ACHX5T_000572"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR8465899.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(43336..44262)
                     /gene="allS"
                     /locus_tag="ACHX5T_000573"
     CDS             complement(43336..44262)
                     /gene="allS"
                     /locus_tag="ACHX5T_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFR8465900.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            44492..44974
                     /gene="allA"
                     /locus_tag="ACHX5T_000574"
     CDS             44492..44974
                     /gene="allA"
                     /locus_tag="ACHX5T_000574"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFR8465901.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            45052..45867
                     /gene="allR"
                     /locus_tag="ACHX5T_000575"
     CDS             45052..45867
                     /gene="allR"
                     /locus_tag="ACHX5T_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFR8465902.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            45957..47738
                     /gene="gcl"
                     /locus_tag="ACHX5T_000576"
     CDS             45957..47738
                     /gene="gcl"
                     /locus_tag="ACHX5T_000576"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFR8465903.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            47751..>48023
                     /locus_tag="ACHX5T_000577"
     CDS             47751..>48023
                     /locus_tag="ACHX5T_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFR8465904.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        11424 a        12502 c        12785 g        11312 t
ORIGIN      
        1 atgagatcct gagttggtgg ttcaattact ccttaatgtt cgtaggttat gcataaaaag
       61 gccgcttacg cggccttagt gattacatgt tgtatcaatg atgatcgaca gtatggctgt
      121 gctcgatatc ttcattcttg cggctaaagc ggcggcgaac caccacaaag aataccggaa
      181 cgaagaagat tgccagtacc gttgcggtca ccatcccgcc cattacaccg gtacctactg
      241 cgttctgcgc gccggaacca gcaccagtac tgataaccag cggcataacg ccgaggataa
      301 acgccagcga ggtcatcagg atcggacgta aacgcatccg caccgcatca agcgtcgctt
      361 caatcagacc tttaccttct ttatccatca agtctttggc gaattcgacg ataagtatcg
      421 cgttcttcgc cgacaaccca atggttgtga gcagtcctac ttggaagtaa acgtcattgg
      481 tcaggccacg gaaggtggca gccagcaacg caccgataac ccccagcgga acgaccagca
      541 taacggagaa cggagtcgac cagctctcgt acagcgccgc cagacacagg aatacgacaa
      601 tcaacgaaat cgcgtacagt gaaggtgcct ggttgccgga gagacgttcc tgataggaca
      661 tccccgtcca gtcatagcca acaccggtag gcagtttgct cgccagttgt tccatcagct
      721 ccattgcttc accggtactt ttacccggtg ccgcctggcc taagatttcc atggatggca
      781 ggccgttgta acgttccaga cgcggcgaac cgtactccca acgagaagag gagaacgccg
      841 agaatggcac catctgacca tcagcagcac gaacatacca gtcgccgata tcatccggca
      901 gcatacggta tttcgcttct gacatgacgt aaactttctt cacacgaccg cggtcgataa
      961 agtcgttcac atagctaccg ccccatgcag cgcccagagt ggtgttaatg tcgttgatag
     1021 aaacacccag cgcctgcgct ttttcctggt cgatatcaat cttaaactgc ggggtatctt
     1081 ccagaccgtt tggacgtacg ctggtcagca tatcagggtg cttcgctgct tctgcaagca
     1141 gctggttacg cgcctgagtc agtttttcgt gaccaaggcc agcctggtca atcagctcaa
     1201 agtcaaagcc ggttgcagta cccagttcca cgattgcggg caggttaaag gcgaaaacca
     1261 tcgcatcttt gatttgcgag aaagcgcgtg ttgcacgcat ggtaatcgct tcaactttgt
     1321 tttcttcgcc cggacgatcg gcccagtcct tcaaggaaac gaacgcaata ccggtattct
     1381 gaccacgtcc cgcaaagccg aagccgttaa cggcgaacac cgactcaacg ttgttctttt
     1441 ctttggtcag atagtaatgc gttacctcat tgagcacttt ctgcgtacgt tcctgcgttg
     1501 cacctgctgg cagctgaacc atggtcataa atacgccctg gtcctcatct ggcaagaagg
     1561 agcttggcag acgcacgaac agataggcca tgccgaccac gatgatcaga tacagcacta
     1621 ggtaacgccc cgtactgcgc agaataccgc ctacgctgtc ggtgtagtgg tgcgtgctct
     1681 tctcgaacat gcggttcaac cagccgaaga agcctttttt accttccccg tgatcgcctt
     1741 tggcaatcgg tttcagcatg gtggcacaaa gagccggagt caggatcaac gctaccagta
     1801 ccgacagcgc cattgctgaa acaatggtaa tagagaactg acgatagata gcaccagtag
     1861 aaccgccaaa gaaggccatc ggtacgaata ccgccgacag taccatcgcg ataccgacca
     1921 gagcgccctg aatctgcccc atcgacttac gggtagcttc ttttggcggc aaaccttctt
     1981 ccgccataac acgctccacg ttttctacca caacgatagc gtcatccacc aacaagccga
     2041 tggcgagcac catcccgaac attgttagcg tgtttatcga gaagccaaag gcggcaagga
     2101 cggcaaaggt cccgagcaat accaccggta cggcaatggt cggaatcaac gtcgcgcgga
     2161 agttctgcag gaacagatac ataaccagga acacgaggat gatcgcttcg accagcgttt
     2221 taaccacttc gtgaatagag attttcacga acggcgtggt gtcgtacggg taaacaattt
     2281 tcagacccga cgggaagaac ggttccatct tcgccagttc agcacggatt gccgcagcgg
     2341 tatccagcgc gtttgcaccg gtcgccagct tgatccccag accggaagca ggttggccgt
     2401 taaactctgc gatgatgtcg tagttctcac cacccagctc aatcttcgcc acatcacgca
     2461 gcagtacgcg ggaaccatcc tgattcactt tcagcaggat tttgccgaac tcttcagtag
     2521 aggtcagacg cgtctgagca ataatagagg cgttaagctg ttggcctttc accggcggcg
     2581 taccaccgag ctgacccgcc gcaacctggg cgttctgcgc tttgatggcg gtaatgacat
     2641 caaccggcgt tagctggaat ttgttcagct cattcgggtt catccagata cgcatcgcgt
     2701 actgtgaacc gaacaactga acatcaccca cgcccgacgt acggctgatg gcatctttca
     2761 tattcgccgc tacgtagtca gagatatcct cctgcgtcat ggtgccatcg gtgttgataa
     2821 cgccgacaac catcaggaag ctgctggatg atttctcaac gctcacccct tgctgctgaa
     2881 cttcttgcgg cagcaacggc atcgccagct gcagtttgtt ctgcacctga acctgcgcga
     2941 tatccgcatc agtaccagac tcaaaggtca gggtgatctg cacggtaccc gtggagtcac
     3001 tgttagagga catgtacatc aggttatcga taccgttcat attctgttcg ataacctgtg
     3061 tcaccgtgtc ctgcactgtt ttcgcatcag cgccggggta ggaggcggag atcgttactg
     3121 ccggcggtgc aatcgtagga tattgcgcca ccggcagttt gaggatcgcc agcccccctg
     3181 ccaacatgat gataatggcg atcacccacg caaaaatcgg gcgatcgata aagaaattag
     3241 gcatgtctta acggctcctg tttaagttaa gacttggact gttcaggctg agcaccgctt
     3301 gcggcttgct ggttattatc agcggtaact tcttgtgctt ttacctggac accaggacgc
     3361 actttctgca gcccacttat tactacgcga tcgcctgctt tcagaccttc tgtcaccagc
     3421 cacttatcgc caatagcctg gcttgcaacg atcggacggg tttccacttt gtcatccgcg
     3481 ccaactacca gtacggtggc atcaccacgc ggcgtacggg ttacgccctg ttgcgggact
     3541 aaaatagcgt ttggattaag cccttcttcc agacgtgcgc gcacgaacat acccggcagc
     3601 agagtgtgat ccgggttcgg gaagatagcg cgtagggtga tagacccagt ggtctgatca
     3661 acggtaacgt cagagaattc cagcgtaccg tcctgcggga acttaatgcc gtcactggtg
     3721 atcagcgaca ctttggcttt gccgttctct tgtttcagcg tgccattcgc cagttcctgt
     3781 ttcaggcgaa ggaagtcgtt gctggactgg gtcacatcaa cgtagatcgg atcaagttgc
     3841 tgcacggttg ccagcacagt cgcctgaccg ttctgtacca atgcgccttc cgtcacgttc
     3901 gacttaccaa tgcgaccgct aatcggagag gtgactttgg tgtaagccag attgatccgc
     3961 gcagtttcaa cggcagcttt cgccgcagtt accgcagcat tcgcctgttg cgcatcagcc
     4021 agagcctgat cgtacccttg cttactgatg tactgagtac cgagcagttt ctgataacga
     4081 ttcaccgtca attgcgcgat attggctgca gcctgggctt tcgccagatc acctttcgca
     4141 ctgtcgtatg tcgcctgata ggtcgcagga tcaatctgat agagagagac accagcttcg
     4201 atgtcgctac cttctttgaa attacgcttc aggataatcc cgctaacttg aggacgaact
     4261 tctgcgatcc ggtaggcact ggtgcgacct ggaagctcgg ttgtgatctg cagaggttca
     4321 gttttgactg ttactacgcc aacggcgggc atctgctggc caccttgttg ggcctgtttg
     4381 tcgtcacatc ctgttagggc taagctgcct gagagcatca gaacgaccgc cagaggcgta
     4441 aaccctctgt ttttgttcat atgtaaacct cgagtgtccg atttcaaatt ggtcaatggt
     4501 caaaagttaa taaacccatt gctgcgttta tattatcgtc gtgctatggt acatacattc
     4561 acaaatgtat gtaaatctaa cgcctgtaaa ttcacgaaca tatggcacga aaaaccaaac
     4621 aagaagcgca agaaacgcgc caacacatcc tcgatgtggc tctacgtctt ttctcacagc
     4681 agggggtatc atccacctcg ctgggcgaga ttgcaaaagc agctggcgtt acgcgcggtg
     4741 caatctactg gcattttaaa gacaagtcgg atttgttcag tgagatctgg gaactgtcag
     4801 aatccaatat tggtgaacta gagcttgagt atcaggcaaa attccctggc gatccactct
     4861 cagtattaag agagatatta attcatgttc ttgaatccac ggtgacagaa gaacgacgtc
     4921 gattattgat ggagattata ttccacaaat gcgaatttgt cggagaaatg gctgttgtgc
     4981 aacaggcaca acgtaatctc tgtctggaaa gttatgaccg tatagaacaa acgttaaaac
     5041 attgtattga agcgaaaatg ttgcctgcgg atttaatgac gcgtcgggca gcaattatta
     5101 tgcgcggcta tatttccggc ctgatggaaa actggctctt tgccccgcaa tcttttgatc
     5161 ttaaaaaaga agcccgcgat tacgtcgcca tcttactgga gatgtatctc ctgtgcccca
     5221 cgcttcgtaa tcctgccact aacgaataac cctgaatctg actccaggat ttttcctgga
     5281 cattttcgtc gttgctattc tggttcactg cgtcgtgata ttcttgcggt ttgacttttt
     5341 caggtcgttc ttcaggttca gaaaccttca ttcatcatga ctatgttcca gtattacaaa
     5401 cgatcacggc attttgtttt ttcagcattt attgcttttg tttttgtctt gttatgccag
     5461 aacacggcgt ttgcgcgggc gtcatcgaat ggtgatctgc cgacaaaagc ggacctgcag
     5521 gcgcaacttg actcactaaa taaacaaaaa gatctttctg ctcaggacaa actggtgcag
     5581 caggatctga cagatacatt agccaccctc gataaaatcg atcgcataaa agaagagaca
     5641 gttcagctac ggcaaaaagt cgctgaagcg ccggaaaaaa tgcgccaggc gaccgcggcg
     5701 ttaacagcac ttagcgatgt cgataacgac gaagaaacgc gcaaaattct gagtacgctt
     5761 tcgttgcgcc agctggaaac tcgcgttgcc caggcgctgg acgatttgca aaacgcacaa
     5821 aacgatctgg cgtcttataa cagccagctg gtttcgttac agacgcagcc cgaacgcgtg
     5881 caaaatgcga tgtataacgc ttcgcagcag ctgcaacaaa ttcgcagtcg tctggatggg
     5941 actgatgtcg gcgagactgc cttacgtccc agccagaaag tgttaatgca ggctcagcag
     6001 gcgttgctga atgcggagat tgaccagcag cgtaaaagcc tggaagggaa caccgtcttg
     6061 caggatacct tgcaaaagca acgtgattac gtgacggcga acagcgcccg tctggagcac
     6121 cagttgcagc tgttgcaaga agcggtaaac agcaagcgcc tgactttaac tgaaaaaacg
     6181 gcgcaggaag ccgtctcccc ggatgaagcc gcgcgtattc aggctaatcc gctggtgaaa
     6241 caggaactgg aaattaacca gcagttaagt cagcgtctga ttaccgcgac tgaaaacggt
     6301 aatcagttga tgcagcaaaa cattaaagtc aaaaactggc tggagcgggc gctgcaatcg
     6361 gaacgaaata ttaaagagca gattgccgtc ctgaagggca gcctgctgtt gtctcgtatc
     6421 ctttaccagc aacaacaaac gctgccctcg gcggatgaac tggaaaacat gaccaaccgc
     6481 atcgcggatt tgcgtctcga acagtttgaa gttaaccagc agcgtgatgc actcttccag
     6541 agcgatgcgt tcgtcaacaa actggaagaa ggtcacacca acgaagtcaa cagcgaagtt
     6601 cacgatgcgt tattgcaagt ggttgatatg cgtcgcgaat tgctggatca actcaacaaa
     6661 cagttgggta accagctgat gatggccatt aacctgcaaa tcaaccagca gcagttaatg
     6721 agcgtatcga aaaacctgaa atccatcctg actcagcaaa tcttttgggt gaacagtaac
     6781 cgtccaatgg actgggactg gatcaaagcg ttcccgcaaa gcctgaaaga tgaatttaag
     6841 tcgatgaaaa tcacggtgaa ctgggaaaaa gcctggcctg ccgtttttat cgctttcctc
     6901 gccggtttgc cgctgctgtt gattgccggg ctgatccact ggcgtctggg ctggctgaaa
     6961 gcgtatcaac agaaactggc ttccgctgtg ggttccctgc gtaacgatag ccagctcaac
     7021 acaccaaaag cgatccttat cgacctgatc cgcgcgctgc cggtgtgcct gattattctc
     7081 gcggttggcc tgattctgtt gaccatgcag ctcaacatca gcgaactgct atggtcgttc
     7141 agtaaaaaac tggcgatttt ctggctggtg tttggcctgt gctggaaggt actggagaaa
     7201 aatggcgttg ccgtgcgtca cttcggcatg ccggagcagc agaccagcca ctggcgtcgg
     7261 caaattgtcc gcatcagtct cgcgttgctg cctatccatt tctggtctgt ggtggcagaa
     7321 ctttccccgc tgcatctgat ggatgatgtg ctggggcagg cgatgatttt cttcaacctg
     7381 ctgctgattg ccttcctggt atggccaatg tgccgcgaaa gctggcgtga taaagagtcg
     7441 cacaccatgc gactagtcac cattaccgtg ctgtcgatta tcccgattgc attgatggtg
     7501 ctgactgcca cgggttattt ctacaccacg ctgcgcctct ccgggcgttg gattgaaacc
     7561 gtttatctgg tgatcatctg gaacttgctc taccagacgg tattgcgcgg cttaagcgta
     7621 gcggcgcggc gtatcgcctg gcgtcgtgcg ctggcgcgtc ggcagaatct ggtgaaagag
     7681 ggcgcagaag gtgccgaacc gccggaagaa cccaccattg cactggagca agttaaccag
     7741 cagacgctgc gtattaccat gttgctgatg tttgcgctgt tcggtgtcat gttctgggca
     7801 atttggtccg atttgatcac cgtgttcagc tatctcgaca gcatcacgct ctggcattac
     7861 aacggcactg aagctggcgc tgcggtggtg aaaaacgtca ccatgggcag tctgttgttt
     7921 gcgattatcg cctcaatggt ggcctgggcg ttgattcgca acctgcctgg tttactggaa
     7981 gtgctggtgc tctcgcgact gaatatgcgc cagggcgcgt cgtatgccat tactaccatc
     8041 cttaactaca tcattattgc cgttggtgcg atgacggtgt tcggatcgct gggcgtctct
     8101 tgggataaac tccagtggct ggccgcagca ttatccgtag gtcttggttt tggtttacaa
     8161 gaaattttcg gtaacttcgt ctccggtttg atcattctgt tcgaacgtcc ggtgcgtatt
     8221 ggcgatacgg taaccattgg tagcttctcg gggacggtaa gtaagatccg tattcgtgcg
     8281 acaacgatta ccgatttcga tcgcaaagaa gtgatcatcc cgaacaaagc gtttgttacc
     8341 gagcgtctga tcaactggtc gttgactgac actactacgc gtctggtgat ccgtctcggc
     8401 gtggcctatg gctccgatct ggaaaaagtg cgtaaagtgt tactgaaggc ggcgactgag
     8461 cacccaaggg tgatgcacga accaatgccg gaagtcttct ttacggcatt tggtgccagc
     8521 acgttggatc atgagctgcg tctgtatgtg cgtgaactgc gtgaccgtag ccgtactgtc
     8581 gatgagctga accgtactat cgatcagctg tgccgtgaaa acgacatcaa cattgccttt
     8641 aaccagcttg aagtgcatct gcacaacgag aagggcgatg aggtgacgga agtaaaacgc
     8701 gactacaaag gcgacgatcc gacaccagcg gttgggtaat aaccaaaggg ggcgacattt
     8761 agttgccccg agattactga caaagtgcgc gttgttcatg ccggatgcgg cgtgaccgcc
     8821 ttatccggcc tacgaaaccg caagaattca atatattgca ggagcggtgt aggcctgata
     8881 agcgtagcgc atcaggcagt tttgcgtttg cccgcaatct taggggacat ttagcggccc
     8941 catttatttc tcacttttcc gcctcatcat cgtgcgttaa tttcttttca taatcacgct
     9001 ttacaatatc cagcgcgcgc agcacggtac tggcagggat ctgattttcc tccagcagca
     9061 caatcaaatc gacagccagt ttgacatcgt caggggcatt ttcaagtgac atactctttc
     9121 cattgctagc gggttaagcg cgctagcctg ttttcgattt tttccagcgc atggcggcag
     9181 cgtgccaggc gcgcttcata ggcttccact tcacgatgca gcgtttgctg ttccacgaga
     9241 tcggtcaccc gcgccaggcg ggctctgcgt tcggcaacca tctcacgtag ccgccgctca
     9301 aaatcctgat gctgaatacg tttacgctgc cagcgggaaa ttttcggtgg cgcactgtcc
     9361 cactcgcgca atgaccaggc ggaggcttca cgcgcgatgg cttccagttg tgccgccaga
     9421 tgttccgcca gccaggccac ttgcggcagc tgttgctgct caactgcatg acgaagcgca
     9481 gccagattat cgcccgcctc gtcgagacaa gactgtagtg ttgtcgcacg agtctgaaaa
     9541 agatgcctgt cgaaacgagc acttagcgtg gcgaactgtg acaccggggc acaacgctga
     9601 cgcagcgtag cgagctgtcc ttccagtttt tccagcagca gggcggtttt cacgataaga
     9661 cctcaatgaa aatgataatt gttatgctaa agtagccact atgtaagctg acaacactca
     9721 atatgcaacg aatcatttta atcatcattg gctggctggc ggtagtgctg ggtacgctgg
     9781 gcgtggtatt accggtatta ccgacgacgc cgtttatcct gctggcggcc tggtgctttg
     9841 cccgttcttc cccgcgcttt cacgcctggt tgctgtaccg ctcatggttt ggcagctatc
     9901 tacgtttctg gcagaaacat catgcgatgc cgcgcggcgt caaaccgcgg gcgattttgc
     9961 ttattttgct cacgtttgcc atttctctgt ggttcgtcca gatgccatgg gtgcgcatca
    10021 tgttgctggt aattctcgcc tgtttgcttt tctatatgtg gcgaattccg gtgattgatg
    10081 aaaagcaaga aaagcactga agcacaacaa tcgcagttgc aattattgcg tacagccagt
    10141 acattctggc gttttcgagc acaggcgcag gcggtcaaag gttaaacaac agttactttt
    10201 gatacgttta aaacgcgccg tgagtaccac cgtaacaagc aggcatacac ttatgaccgc
    10261 gactgcacag cagcttgagt atctcaaaaa tagcatcaaa agcattcagg actacccaaa
    10321 acccggcatt cttttccgcg atgtcaccag cttactggaa gacccgaaag cttacgctct
    10381 cagcatcgac ttgctggttg agcgttacaa aaatgcgggc attaccaaag ttgtcggcac
    10441 cgaagcgcgt ggcttcttgt ttggcgctcc ggtagctctg ggtctgggcg ttggctttgt
    10501 accggtccgt aaaccgggca aactgccgcg tgaaaccatc agtgaaaatt acgacctgga
    10561 atacggcacc gatcagctgg aaatccacgt tgatgccatc aaaccgggcg acaaagttct
    10621 ggtggtggac gatctgctgg caaccggcgg cactatcgaa gcgaccgtta aactgatccg
    10681 tcgtctgggt ggtgaagtgg ctgacgctgc gttcattatc aacctgtttg atctcggcgg
    10741 cgaacagcgt ctcgaaaaac agggcattac cagctacagc cttgtcccgt tcccgggcca
    10801 ttaattatcg ccagtcttgt gctgcccacg ctacggacag cacaagatgt gcattcagcc
    10861 tcgccgttct gacggggctg tgttagcatt accccttcgt gaatccacct tccagcgttt
    10921 cagagcctgc caatgagtta tcaggtttta gcccgaaaat ggcgcccaca aacctttgct
    10981 gacgtcgtcg gccaggaaca tgtgctgacc gcactggcga acggcttgtc gttagggcgt
    11041 attcatcatg cttatctttt ttccggcacc cgtggcgtcg gaaaaacctc tatcgcccga
    11101 ctgctggcga aggggctaaa ctgcgaaacc ggcattaccg cgacgccgtg cggcgtgtgc
    11161 gataactgtc gtgaaatcga gcaggggcgc tttgtcgatc tgattgaaat cgacgccgcc
    11221 tcgcgcacca aagttgaaga tacccgcgac ctgctggata acgtccagta cgctccggcg
    11281 cgtggtcgtt tcaaagttta tctgatcgac gaagtgcata tgctgtcgcg ccacagcttt
    11341 aacgcactgt taaaaaccct tgaagagccg ccggagcacg ttaagtttct gctggcgacg
    11401 accgatccac agaaattgcc ggtgacgatt ttgtcacgct gtctgcaatt tcatctcaag
    11461 gcgctggatg tcgagcaaat tcgccatcag cttgagcaca tcctcaatga agaacatatc
    11521 gctcacgagc cgcgggcgct gcaattgttg gcgcgcgccg ctgaaggcag cctgcgagat
    11581 gccttaagtc tgaccgacca ggcgattgcc agcggtgacg gccaggtttc aacccaggcg
    11641 gtcagtgcga tgctgggtac gcttgacgac gatcaggcgc tgtcgctggt tgaagcgatg
    11701 gtcgagggca acggcgagcg cgtaatggca ctgattaatg aagccgctgc ccgtggtatc
    11761 gagtgggaag cgttgctggt ggaaatgctc ggcttgttgc atcgtattgc gatggtacaa
    11821 ctttcgcctg ctgcacttgg caacgacatg gccgccatcg agctgcggat gcgtgaactg
    11881 gcgcgcacca taccgccgac ggatattcag ctttactatc agacgctgtt gattggtcgc
    11941 aaagaattac cgtatgcgcc ggaccgccgc atgggcgttg agatgacgct gctgcgcgcg
    12001 ctggcgttcc atccgcgtat gccgctgcct gagccagaag tgccacgcca gtcctttgca
    12061 cccgtcgcgc caacggcagt aatgacgcca accctggtgc cgccgcaatc agcgccgcag
    12121 caggcaccga ctgtaccgct cccggaaacc accagccagg tgctggcggc gcgccagcag
    12181 ttgcagcgcg tgcagggagc aaccaaagca aaaaagagtg aaccggcagc cgctacccgc
    12241 gcgcggccgg tgaataacgc tgcgctggaa agactggctt cggtcaccga tcgcgttcag
    12301 gcgcgtccgg tgccatcggc gctggaaaaa gcgccagcca aaaaagaagc gtatcgctgg
    12361 aaggcgacca ctccggtgat gcagcaaaaa gaagtggtcg ccacgccgaa ggcgctgaaa
    12421 aaagcgctgg aacatgaaaa aacgccggaa ctggcggtga agctagcggc agaagccatt
    12481 gagcgcgacc cgtgggcggc acaggtgagc caactttcgc taccaaaact ggtcgaacag
    12541 gtggcgttaa atgcctggaa agaggagagc gacaacgcag tatgtctgca tttgcgctcc
    12601 tctcagcggc atttgaacaa ccgcggtgca cagcaaaaac tggctgaagc gttgagcatg
    12661 ttaaaaggtt caacggttga actgactatc gttgaagatg ataatcccgc ggtgcgtacg
    12721 ccgctggagt ggcgtcaggc gatatacgaa gaaaaacttg cgcaggcgcg cgagtccatt
    12781 attgcggata ataatattca gaccctgcgt cggtttttcg atgcggagct ggatgaagaa
    12841 agtatccgcc ccatttgatc gtaagcacag cttacgttcg tcatccttaa cgtgattgag
    12901 agagaaacct atgtttggta aaggcggtct gggtaacctg atgaagcaag cccagcagat
    12961 gcaagaaaaa atgcagaaaa tgcaggaaga gatcgcgcag ctggaagtta ccggcgaatc
    13021 tggcgcaggt ctggtaaaag tgaccatcaa cggtgcacac aactgccgtc gcgtagagat
    13081 cgacccgagc ctgctggaag acgacaaaga gatgctggaa gacctggtgg ctgcagcatt
    13141 caacgacgca gcacgtcgta ttgaagaaac gcagaaagaa aaaatggcct ctgtatcctc
    13201 cggaatgcag ctgccgcctg gctttaagat gccgttctga tgcaaaccag cccgctgtta
    13261 acacagctta tggaagcact gcgctgtctg ccgggcgttg gcccgaagtc ggcgcagcgt
    13321 atggcgttca cgctgcttca gcgcgatcgt agcggcggga tgcgtctggc gcaggcgctc
    13381 acccgggcga tgtcggaaat cggccactgc gccgattgcc gcactttcac cgaacaggaa
    13441 gtctgtaaca tctgttcgaa tccgcgtcgt caggaaaacg gtcaaatctg tgtggtggag
    13501 agtccggcgg acatctacgc cattgagcag acggggcagt tttctggtcg ttattttgtg
    13561 ttgatggggc atctgtcacc gctggacggc atcggtccgg atgatatcgg gcttgatcgt
    13621 ctggaacagc gtctggcaga ggaaaaaatc actgaagtga tcctcgccac caatcccacg
    13681 gttgaaggtg aagctaccgc taactacatt gccgagcttt gcgcgcaata tgacgtggaa
    13741 gccagccgaa tcgctcatgg cgtaccggtt ggcggcgaac tggaaatggt cgatggcacc
    13801 acgctgtcac actccctcgc cgggcgtcat aagattcgtt tttaagcaaa cgagagcagg
    13861 agcacctgct ctcgcttgaa attattctcc cttgtcccca tctctccctc atcctgtttt
    13921 taaccttaaa atggcattat tgaggtagac ctacatgaaa ggacaagaaa ctcgtggttt
    13981 tcagtcagaa gtgaaacagc ttctgcacct gatgatccat tctctctatt ccaataaaga
    14041 aatcttcctg cgtgagctta tctctaacgc ctccgatgcg gcggacaagc tgcgtttccg
    14101 tgcgctatct aacccggacc tgtacgaagg tgatggcgaa ctgcgcgttc gtgtctcttt
    14161 cgataaagac aagcgcacgc tgaccatctc cgataacggc gtggggatga cccgcgacga
    14221 agtgattgac catctgggga ctatcgctaa atccggtacc aaatcattcc tcgaatccct
    14281 gggttctgac caagcgaaag acagccagct gatcggtcag tttggtgttg gtttctactc
    14341 tgcgtttatc gtggccgaca aagtgaccgt gcgtactcgc gcggcaggcg aaaaaccaga
    14401 aaacggcgtc ttctgggaat cggctggcga aggtgaatat accgttgccg atatcaccaa
    14461 agaagatcgt ggtactgaaa tcaccctgca tctgcgtgaa ggcgaagacg agttcctcga
    14521 tgactggcgc gtgcgttcca tcatcagcaa atactccgac catatcgcgc tgccggtaga
    14581 gatcgaaaaa cgcgaagaga aagatggcga aaccgttatc tcctgggcaa aaatcaacaa
    14641 agcgcaggcg ctgtggactc gtaacaagtc ggaaatcacc gatgaagagt ataaagagtt
    14701 ctacaaacac atcgcccacg actttaacga tccgctgacc tggagccaca accgcgttga
    14761 aggtaagcag gagtacacca gcctgctgta catcccgtcc caggctccgt gggatatgtg
    14821 gaaccgtgat cataaacacg gcctgaaact gtacgttcaa cgcgtgttta tcatggacga
    14881 cgctgaacag ttcatgccga actatctgcg cttcgtgcgt ggtctgattg actccagcga
    14941 tctgccgctg aacgtttccc gtgaaatcct ccaggacagc acggtaacgc gtaacctgcg
    15001 caatgcgctg accaagcgtg tgctgcaaat gctggaaaaa ctggcgaaag acgacgcgga
    15061 aaaataccag accttctggc aacagtttgg cctggtactg aaagaaggtc cggcggaaga
    15121 tttcgctaac caggaagcga tcgccaaact gctgcgtttt gcttctactt ataccgattc
    15181 ttctgcgcag accgtgtctc tggaagacta cgtttcccgc atgaaagaag ggcaggagaa
    15241 aatctactac atcactgcag acagctatgc ggcagcgaag agcagcccgc acctggaact
    15301 gctgcgtaag aaaggcatcg aagttctgct gctttccgac cgcatcgatg agtggatgat
    15361 gaactatctg actgagttcg acggtaaacc gttccagtcg gtgtctaaag ttgacgagtc
    15421 gctggaaaaa ctggctgacg aagttgatga gagcgcgaaa gaagcggaga aagcactgac
    15481 tccgttcatc gaccgcgtga aagctctgct cggcgagcgc gtgaaagatg tccgtctgac
    15541 tcaccgtctg accgatacgc cagcgatcgt ttcgaccgac gcggacgaaa tgagcactca
    15601 gatggcgaaa ctgttcgctg cggcgggcca gaaagtgcca gaagtgaaat acatcttcga
    15661 actgaacccg gatcacgtac tggtgaaacg tgcggcagat actgaagatg aagccaagtt
    15721 cagcgagtgg gtagaactgc tgctggatca ggcgctgctg gcagaacgcg gcacgctgga
    15781 agatccgaac ctgtttattc gtcgtatgaa tcagctgctg gtttcctgat gtcatgccgg
    15841 atgacctttg tgtcatccgg catttttctt ttcatcatct gtactttccg caaattatct
    15901 cgccattaac cgtttcagcc tcaggtgcct ttcttgaggc aatcgcctgt tggtggtatc
    15961 gtttatcgct ttttcaaaaa aatcgacaca ttttaagggg attttcgcaa tgcgtatcat
    16021 tctgcttggc gctccgggcg cggggaaagg gactcaggct cagttcatca tggagaaata
    16081 tggtattccg caaatctcca ctggcgatat gctgcgtgct gcggtcaaat ctggctccga
    16141 gctgggtaaa caagcaaaag acattatgga tgctggcaaa ctggtcaccg acgaactggt
    16201 gatcgcgctg gttaaagagc gcattgctca ggaagactgc cgtaatggtt tcctgttgga
    16261 cggcttcccg cgtaccattc cgcaggcaga cgcgatgaaa gaagcgggca tcaatgttga
    16321 ttacgttctg gaattcgacg taccggacga actgattgtt gatcgtatcg taggccgccg
    16381 cgttcatgcg ccgtctggtc gtgtttatca cgttaaattc aatccgccga aagtagaagg
    16441 caaagacgac gttaccggtg aagaactgac tacccgtaaa gacgatcagg aagaaaccgt
    16501 gcgtaaacgt ctggttgaat accatcagat gactgcaccg ctgatcggct actactccaa
    16561 agaagcggaa gcgggtaaca ccaaatacgc gaaagttgac ggtaccaagc aggttgctga
    16621 agttcgcgct gatctggaaa aaatcctcgg ctaatcaagc cgcagcagat ctgctttcgg
    16681 gcaggtctgc ttatccacct caggcaatac ctctcacagc aattagttct tcttcctcac
    16741 ttttccgcta caattatcaa caagttgaat cgataagagg cggtaatgcg tcagactaaa
    16801 accggtatcc tgctggcaaa cctgggtacg cccgatgccc ccacacctga agcggtaaaa
    16861 cgctatctga aacaattttt aagcgacaga cgcgtggttg atacctcacg gttgttatgg
    16921 tggccgttgc tgcgcggcgt gattttgccg ctgcgctcgc cgcgtgtggc gaagctgtac
    16981 gcctctgtct ggatggaaga cggctcgccg ctgatggttt acagccgcca gcaacagcag
    17041 gcgttggcac aacgtttacc ggatacgcca gtggcgctgg gcatgagcta cggctcgcca
    17101 tcactggaaa gctccgtcga tgaactcctg gcagagcatg tagatcatat tgtggtgctg
    17161 ccgctttatc cgcaattctc ctgttctacg gtcggtgcgg tatgggatga actggcacgc
    17221 attctggcgc gcaaacgtag cattccgggg atatcgttta ttcgtgatta cgccgataac
    17281 cacgattaca ttaatgcact ggcgaacagc gtacgcgctt cttttgccaa acatggcgaa
    17341 ccggatctgc tgctgctctc ttatcatggc attccccagc gttatgcaga tgaaggcgat
    17401 gattacccgc aacgttgccg cacaacgact cgcgaactgg cttccgcact ggggatggta
    17461 ccggaaaaag tgatgatgac ctttcagtcg cgctttggcc gggaaccctg gctgatgcct
    17521 tataccgacg aaacgctgaa aatgctcgga gaaaaaggcg taggtcatat acaggtgatg
    17581 tgcccgggct ttgctgcgga ttgtctggag acgctggaag agattgccga gcaaaaccgt
    17641 gaggtcttcc tcggtgcggg cgggaaaaaa tatgaatata ttccagcgct taatgccacg
    17701 ccggaacata tcgaaatgat ggctaatctt gttgccgcgt atcgctaaag ctgagcggta
    17761 aagaactgag cgccgtcgcg aagagcctcg tcggcggttt tcatcatccg tgaataatgc
    17821 aaaaaggcat gcagcgtacc tgggtagagt ttgaactcac agggctgctg atgcgccgct
    17881 aacgtctagt aaagcagacg gctgtcatcc agcagcggat cgaactccgc cccggcaata
    17941 aaacagggcg gaacttcgcg agtgagatca ttattaaaca gacagtaata cggtgactcg
    18001 cggtccgcgt cgttgcttaa atacgcctct tcatacatct gcaaatcctg ttgcgttaag
    18061 ccatcccaga caccgcccaa cagacgacga gtcacggaat cccgtaatcc gtaaagccca
    18121 taccacagca aaacacccgc aattttaccg caatcgatct gtttatcacg caaccacaac
    18181 gcactggcga gcgccagcat ggcacctgcg gaatcaccgg caaagccaat gcgggacata
    18241 ttgatttgat aatcctccgc ctgctggtgg aaataacaac aagcagccac aatttcctct
    18301 atcgcttgtg gaaaacgcgc ttcaggtgaa agggtgtaat taataccaat caccgtacat
    18361 tggctgtagc ttgccagcag gcgcatgatg cgatcgtggg tatcgagatt gccgagaata
    18421 aaaccgcctc catgcaaata aaatagcgtc gccgggctat ctggctgcgg acaaaagaga
    18481 cgtgtttcca cctgcccata ttttgttgga accatgtaag ctctggttgc catttctgga
    18541 gcgcccgcat tccagaatcg gcgctcaagc gtgtaatact gtcgttgctc agcaatcgtt
    18601 cccgttgcgg gccaggacgg taaatccggc tgaagagtat tcacaacggt cttcatttca
    18661 gcagaaataa ggtccagaac aggtagtttg ttttccggct tcatacaaaa actcctttca
    18721 aattacgtca ttgtaaggaa ccactgccat gaaaatgcga tcccgcctgc tgatattgaa
    18781 actggctgcg tctcgcgcgc tcccgtcaga ttgtgttaac attcgccgct cagttaacca
    18841 cccgtaaaaa caaccatgaa atttcccggt aaacgtaaat ccaaacatta cttccctgta
    18901 aatgcacgcg atccgctgct tcagcaattc cagccagaaa acgaaaccag cgccgcctgg
    18961 gtcgtaggta ttgaccagac gctggtcgat attgaagcga aagtggatga tgaattcatt
    19021 gagcgttatg gattaagcgc cgggcattca ctggtgattg aggatgacgt agccgaagtg
    19081 ctttatcagg agctaaaaca gaaaaacctg attacccatc agtttgcggg tggcaccatt
    19141 ggtaacacca tgcacaacta ctcggtgctc gcggacgacc gttcggtgct gctgggcgtc
    19201 atgtgcagca atattgaaat tggcagttat gcctatcgtt acctgtgtaa cacttccagc
    19261 cgtaccgatc ttaactatct acaaggcgtg gatggtccga ttggtcgttg ctttacgctg
    19321 attggcgagt ccggggaacg tacctttgct atcagtccag gccacatgaa ccagctgcgg
    19381 gctgaaagca ttccggaaga tgtgattgcc ggagcctcgg cactggttct cacctcatat
    19441 ctggtgcgtt gcaagccggg tgaacccatg ccggaagcaa ccatgaaagc cattgagtac
    19501 gcgaagaaat ataacgtacc ggtggtgctg acgctgggca ccaagtttgt cattgccgag
    19561 aatccgcagt ggtggcagca attcctcaaa gaccacgttt ctatccttgc gatgaacgaa
    19621 gatgaagccg aagcgttgac cggagaaagc gatccgttgt tggcatctga caaggcgctg
    19681 gactgggtag agctggtgct gtgcaccgcc gggccaatcg gcttgtatat ggcgggcttt
    19741 accgaagacg aagcgaaacg taaaacccag catccgctgc tgccgggcgc tatagcggaa
    19801 ttcaaccagt atgagtttag ccgcgccatg cgccacaagg attgccagaa tccgctgcgt
    19861 gtctattcgc acattgcgcc gtacatgggc gggccggaaa aaatcatgaa cactaatgga
    19921 gcgggggatg gcgcattggc agcgttgctg catgacatta ccgccaacag ctaccatcgt
    19981 agcaacgtac caaactccag caaacataaa ttcacctggt taacttattc atcgttagcg
    20041 caggtgtgta aatatgctaa ccgtgtgagc tatcaggtac tgaaccagca ttcacctcgt
    20101 ttaacgcgcg gcttgccgga gcgtgaagac agccaggaag agtcttactg ggatcgttaa
    20161 gttatcgtcg gttcgtaggc cagataaggc gttcacgccg catctggcat ttggctctcg
    20221 atgcctgatg cgacgctggc gcgtcttatc atgcctacat atttttcata ttttacatcc
    20281 ggcaaccacc gtttaccccg tcaccacttc acccgccggt ggcgtttcca gcagttccag
    20341 catagtacgg gcaatttcac gttcacccat cactacctga ttcgcgccac gttcggtgat
    20401 atacgtcact tcatcgtcat aatgggcgcg ggcgataatc tcaatatccg ggtttttcgc
    20461 gcgggcagac gccacaatct cacccgcttc ataaccgttg gggatcgtca ggatcagcca
    20521 ttttgcacat tccagatgtg ccagttgcat aatttcttcg ttcgctgcat tgcccaatac
    20581 agcgcggacc ccacgctctc gcagctcatc aacacgggtt cgtgacgtct caatcaccac
    20641 cagcggaata tcagaggcga gcaatttctc ccccagcagg ctgcctacac gaccgtaacc
    20701 caccaatagc gcatggttgc aaatatccac tgggatctgt ttctcttctt cgattgcctc
    20761 ttccagcgtc tgctcttcca gcgtttcggt cttcgccaga tatttctcca gtagtgcgaa
    20821 cagtaccggg ttgagcataa tcgacaggat cgcccctgcc agtaccaggt tttgtccggc
    20881 ctgcggcagt aaattcaatg ccattcccag tcccgccagg ataaacgcga actcaccaat
    20941 ctgcgccagg ctggcggcga tggttaatgc cgtacgttgg gagtgaccaa acagacggac
    21001 aaggaacagg gccgcgaccg atttaccaaa cagaataatt gccagcgtcg ccagcactgc
    21061 cagcggttgc tgaatcagaa ttaacggatc aaacaacatc ccgactgaga caaaaaacag
    21121 caccgcaaac gcgtcgcgca atggcaacgt atcgtgggcg gcacggtgac tcaattcaga
    21181 ctcgttcagt accatcccgg caaagaacgc accgagtgca aaggagacat caaacagctc
    21241 taccgcacca aaggcaatcc ctaacgccag cgccagcact gacagggtaa acagctcgcg
    21301 agaaccggtt gccgcgctgc gtgccataat ccacggcacc agacggcgac ctaccagcat
    21361 cataatggcg ataaatgcga tcaccttgcc gatggtgatc cccatatcga ctgcaagagt
    21421 ggcaaagccc acatcgccct gttccatcat tcctgccact gcgggcagca acaccagcgt
    21481 cagaaccatc accaggtctt ccacaatcaa ccaaccgatg gcgatttgcc cacgctgact
    21541 gtctattaat tgccgttctt caagtgcgcg cagtaacacc acggtactgg cggtggaaag
    21601 acataagccg aacacgatac cggtcattaa cgaccagccc agcacggcgg agagcgccat
    21661 acccagcagc gtcgccacgg ctatctgggc gatcgcgccg ggaatggcga tggactttac
    21721 cgccatcaaa tccttgagcg aaaaatgcaa accgacgccg aacatcagca aaataacgcc
    21781 cagttcagcc agttccggcg caagcttggt atcggcaaca aagcccggag tgaatggtcc
    21841 tgccagcaca cccgctaaca gatatcccac cagaggagaa atacgtagtt tattggccag
    21901 catgccgagg ataaaggcga gcacaaggcc gccaacaatg gtggtgataa gcggggtggc
    21961 gtgatgcatt ccgtctcctt ttcctggtgg ttattgtcca tttttggctg ggaaaaccaa
    22021 aattacaggt aatagtttat gacaatttca ttgatgatgt ttatgaataa ttgttgaatt
    22081 ttgcagaaaa atggaattag ctgcaaaaaa agcacggttc ggaaaacaga aggcggttta
    22141 acagaggaaa ggcttatggc gactgtaaaa cgatgcagcc aaagtttgcc tttggctgca
    22201 atgaaatcag tctttatgcc ggttatcagg caggaatatg gtcaacatcc ccaatagtgg
    22261 caggaaagca cagattttat agactaactc gatgctggtg tgatcggcga taagccccag
    22321 aactgccgct cccagacctc ccatgccgaa agcaaaaccg aaaaagagtc cagaaaccat
    22381 accgatacgt cccggaagta gctcctgagc gtagaccaga atggcagaga atgccgaagc
    22441 gaggataaat ccaataatca ccgttaaaac ccccgtccag tgcaggcggg cgtagggtaa
    22501 aatcagcgta aacggcgcaa cgccgaggat agagccccaa atcacatatt tccgtccaat
    22561 tttatcccct acaggcccgc cgatcaccgt acctgccgca acggcaaaca ggaaggcaaa
    22621 cagatgaagc tgggcattct ggatagataa tccgaatttt tgcatcagat aaaaggtgta
    22681 atagctgctg atgctcgcca tatagaaata tttcgagaaa atgaggatta acagaatgct
    22741 gactgccagt accactttgt tgcgcggcag tggattgatt atcgtcgctt tgggttttcc
    22801 tttattcatt cggtgctgtg ccgagtacca acggctaatt tgcgccaaca ccacgatcgc
    22861 cagcagtgcc gcaagcacaa accaggcaac gttgcctttg ccataaggct cgataatcac
    22921 cgccgccagc aagggtccca gggaactgcc aaagttgccg ccgacctgaa agatagattg
    22981 cgccaggcca tgccgcccgc cggaagccat acgggctacg cgagaagatt ccggatgaaa
    23041 gaccgatgaa ccggtaccga ccagcgccgc tgccagcaga actgcgccaa aactgcccgc
    23101 cagcgcaagc agcaccagac cacttaaggt aaagcacatg ccaattggca acgaccacgg
    23161 catcggatat ttatcggtcc agtagccgac cactggttgc agtagcgaag aggcgagctg
    23221 gaaggtgagg gttatcatgc caatctgcat aaatgtcaga gaaaattctg actgaagcag
    23281 cggataaatc gccagaatca gcgattggat catgtcgttc agcagatgtg agaggctgat
    23341 agcacctaaa ataccaaacg atgttcgggc cttggtcgtt gacgcagccg cgcccgccac
    23401 aggctgggtt tgttcactca ttgccatagg aaagtcactt tttcagggtt gcgatgtaaa
    23461 gaatgatctt atttgtgatt attacccgac taacatacct gtatgcgtcg tctgaaggaa
    23521 gtctcaacgc cgaatacaga atttctaatc tggatgcaga tttatcttca ccggacgcag
    23581 acttgtctat gatgttgcgt catactattt ttcaacacgt tgaaatcagg tcagggagag
    23641 aagtatgaaa ttattgcagc ggggcgtggc gttagcgctg ttaaccacat ttacactggc
    23701 gagtgaaact gctctggcgt atgagcagga taaaacctac aaaattacag ttctgcatac
    23761 caatgatcat catgggcatt tttggcgcaa tgaatatggc gaatatggtc tggcggcgca
    23821 aaaaacgctg gtggatggta tccgcaaaga ggttgcggct gaaggcggta gcgtgctgct
    23881 actttccggt ggcgacatta acactggcgt gcccgagtct gacttacagg atgccgaacc
    23941 tgattttcgc ggtatgaatc tggtgggcta tgacgcgatg gcgatcggta atcatgaatt
    24001 tgataatccg ctcaccgtat tacgccagca ggaaaagtgg gccaagttcc cgttgctttc
    24061 cgcgaatatc taccagaaaa gtactggcga gcgcctgttt aaaccgtggg cgctgtttaa
    24121 gcgtcaggat ctgaaaattg ccgttattgg gctgacaacc gatgacacag caaaaattgg
    24181 taacctggaa tacttcactg atatcgaatt tcgtaagccc gccgatgaag cgaagctggt
    24241 gattcaggag ctgcaacaga cagaaaagcc agacattatt atcgcggcga cccatatggg
    24301 gcattacgat aatggtgagc acggctctaa cgcaccgggc gatgtggaga tggcacgcgc
    24361 gctgcctgcc ggatcgctgg cgatgatcgt cggtggtcac tcgcaagatc cggtctgcat
    24421 ggcggcagaa aacaaaaaac aggtcgatta cgtgccgggt acgccatgca aaccagatca
    24481 acaaaacggc atctggattg tgcaggcgca tgagtggggc aaatacgtgg gacgggctga
    24541 ttttgagttt cgtaatggcg aaatgaaaat ggttaactac cagctgattc cggtgaacct
    24601 gaagaagaaa gtgacctggg aagacgggaa aagcgagcgc gtgctttaca ctcctgaaat
    24661 cgctgaaaac cagcaaatga tctcgctgtt atcaccgttc cagaacaaag gcaaagcgca
    24721 gctggaagtg aaaataggca aaaccaatgg tcgtctggaa ggcgatcgtg acaaagtgcg
    24781 ttttgtacag accaatatgg ggcggttgat tctggcagcc caaatggatc gcactggtgc
    24841 cgactttgcg gtgatgagcg gaggcggaat tcgtgattct atcgaagcag gcgatatcag
    24901 ctataaaaac gtgctgaaag tgcagccatt cggcaatgtg gtggtgtatg ccgacatgac
    24961 cggtaaagag gtgattgatt acctgaccgc cgtcgcgcag atgaagccag attcaggtgc
    25021 ctacccgcaa tttgccaacg ttagctttgt ggcgaaagac ggcaaactga acgaccttaa
    25081 aatcaaaggc gaaccggtcg atccggcgaa aacttaccgt atggcgacat taaacttcaa
    25141 tgccaccggc ggtgatggat atccgcgcct tgataacaaa ccgggctatg tgaataccgg
    25201 ctttattgat gccgaagtgc tgaaagcgta tatccagaaa agctcgccgc tggatgtgag
    25261 tgtttatgaa ccgaaaggtg aggtgagctg gcagtaatcc gaaagtgccg gatgtttgca
    25321 tccggcacaa tgcttaatcg cggcgggcga tatcagcaaa tttggcatcg aggatctttg
    25381 ccagatcgcc tgccgccagt tcgatatcca gtccgcgctt gccgccggaa acataaatag
    25441 tggcaaattc ttgtgcgggg gcgtcgataa tcgttggcag acgttttttc tgccccagtg
    25501 ggctaatccc cccaaccagg tatcccgtcg aacgctgcgc gaccatcgga tcggccatct
    25561 caactttctt ggcacccagc gcttttgcta cttttttaag atccagttga cctgcgaccg
    25621 gcgtaacggc cacggcaagg tgtttcatat caccgttcac tgccaccagc agcgttttgt
    25681 agacctgatc cggattcaaa cctaattttt tgacgacttc atcgccaaaa ttggtttcag
    25741 ccggatcgtg ctcgtaggta tggatttgaa acgaaatctt gtttttttcg agtaatttaa
    25801 ctgcgggtgt catggtaatt ttcctgcctt aaacaaaaaa acgaagcaag catacgcctg
    25861 atggcggtcc aaaaaaaaga gtcatcttgc ctaagagtat tggcaggatg gtgagattga
    25921 gcgacaatcg agttacaccg tcgcttaaag tgacggcata ataataaaaa aatgaaattc
    25981 ctctttgacg ggccaatagc gatattggcc atttttttag cgcaacattt gcggcaaatt
    26041 cccttctcca tacaggtgta gtgcaccgac cgcgaccaca tatcgccccg gcggcatggc
    26101 gcgtaattta tcccgccagg cgagatttcg ctgatgcatc agcacatcgt acagcgactg
    26161 actgaacgta ttgggcagcg ttatatcatt attttgcggc ggtgcattca gccaccagct
    26221 catcatttgt tgcagcaacc gtgcgttggt atgccagtgg gtcagcgtat cgtccagcag
    26281 cgccagtcct ttgtcaggga gctggagcaa catggcaatc tggttttcag ccccttccag
    26341 ttcaatcacg ggtttatgtt gttgcttcgc cgcctgcaat agctggtaat cgataccgta
    26401 ttctgcccgc agccccagtt tttgtgcctg cgtcgcctga agaaccatcg cgatttgcca
    26461 cagcggttgg gtagaaaaga gtgagggaga aatgcccatc tcctggctaa tgtgctgcag
    26521 gttttggagt tgctcctcgc taatgcgctc ttccagcgcc tcgcaggcag gcaaattagc
    26581 aaaaggcgta tcgctggtgg aaacatccgc ctcgacgatc agcgcatcgg cgtttttgag
    26641 ctttttgagc aaacgggtgg gcaggggagc catatcgtgg ctacccatat gaatactgcc
    26701 aatcagatga aaatggcgat tgccggggag ggtgatatcg atggcaggcc aggtgtaatg
    26761 attaccgcgc agcgcggccc aaagcgtttt tacccggtac aacagatcca tacgacctcc
    26821 ctttgtgaaa tatcatgcta gcgcgcggtg agggatggcg caaatggagt tggggacttg
    26881 tttaatgtgt ttgtatgatt cagtatgttc ttgcatcgct attcacaggg aagcaacagt
    26941 taaaaaccat gaaacaggca acaagaaaac cgacgacacc cggagatatt cttctctatg
    27001 aatatctgga accgctcgat ttgaaaatca atgagttagc agagttgctg catgttcatc
    27061 gtaatcgcgt cagtgcactg atcaataaca atcgtaaact caccactgag atggcattcc
    27121 gtctggcgaa agtttttgat accacagtcg attttaggct aaacctccag gcggcagttg
    27181 atctttggga agttgaaaac aacatgcgca cccaggaaga attgggacgg attgaaacag
    27241 tggctgaata tttggcacgc cgtgaagagc gtgcaaaaaa ggtcgcgtaa ggccaaaaca
    27301 tcgcttcatc tcgtaggccg gataaggcgt tcacgccgca tccggcatcc gagcaccatt
    27361 gcctgatgcg acgcttacgc gtcttatcag gcctacaaac ctgtgcctga accgtaggcc
    27421 ggataaggcg tttacgccgc atccggcagt catgcgtcga tgccaaatgc gccgccctaa
    27481 agcagcgcat ccgcaatgac gtacttattc cttcggttta aaccgcagca accggttggc
    27541 gttacttact acggtaattg acgagagcgc cattgccgct ccggcaacta ccgggttaag
    27601 cagtgttcca gtaaacggcc acaaaatacc ggcggcgacc ggaataccga tgctgttgta
    27661 gataaaggca ccgagcaggt tctgcttcat gttgcgcagc gttgcgcggg aaatcgcgag
    27721 cgcatccgca acgcccatca ggctatggcg catcagggta atcgccgcgg tttcaatggc
    27781 aacatcactg ccgccaccca tcgcaatgcc gacatccgcc tgggccagcg ctggcgcgtc
    27841 gttaatgccg tcgcccacca ttgccacctg acgtccttca ctttgcagac gtttgatcgc
    27901 ttcggcttta ccgtccggca gcactccggc aatcacttca tcaattccag cttctttggc
    27961 gatcgcattg gcggtggttg ggttatcccc ggtcaacatc accagacgat atcccgcttt
    28021 atgcaggcgt tgcagcgccg ccacgctatc actacgcaac ggatcgcgta cagccagcag
    28081 ggctactgct ttcccgtcaa tcgccagcag cacaggcgtt gctccttgcg atgcctgagc
    28141 tgaaatatcg gcttcaatcg ctttagtatc gacttgctga tcatttaaca acgcctgatt
    28201 acccagcaat aacgcatgac cttctgcttc accgctcacg cccagcccgc gcaatgtgcg
    28261 gaaaccgttg acctgcggta gctgcatatc gcctgcttta tcaaggatcg ctcgtgccaa
    28321 tggatggctg gaaccttgct ccagcgccgc cgccagacgc agcgcctgcg cttcgtcaac
    28381 atcagcaaat gttttcactg cgacaacctg cggcttccct tcggtcagcg tcccggtttt
    28441 atcgaacacg acggtatcga gtgtactggc gcgttgcagc gcgtcggcgt cccgcaccag
    28501 cacgccaaac tcagccgccc gcccgacgcc ggaaataatc gacatcggcg tcgccagtcc
    28561 cagcgcacac gggcaggcga taatcagcac cgtagtggca atcaccaggg tatagacaat
    28621 ctgcggtgcc ggaccaaaga aataccagat tgccgcactg acaagcgcaa taaccactac
    28681 taccggcaca aatatggctg agattttatc cgccagctga ccgatttctg gcttgctgct
    28741 ctgggcctgg cgcaccatgc gaatgatcct tgatagcgtg gtatggctgc caaccgcact
    28801 ggcacgaaac agcacactgc cgtcctgtac cactgtcccg gcatggacgc tatcgccttc
    28861 gcctttttgc tgcgggattg gttcgcccgt cagcatcgct tcatccagcc atgcttcgcc
    28921 ctgggtaatc tcgccatcta ccggcacgcg atcgccggtc gtcaggcgca gcaacatacc
    28981 tggctgcact tctgccagag gcacgctttt ttcaccttcg tcagtaacca ggcgcgccgt
    29041 cggtggggtt aaatcgagta atttctccag tgccttagaa gaacgctgac gtgcgcgtgc
    29101 ttccagcata tggccgagat tgatcagacc gataatcatc gcactggctt catagtaaag
    29161 atgtcgcgct tccatcggga accactgcgg ccacaggttg acgctcatcg aatagagcca
    29221 cgccacgccc gttcccagcg ccaccagcgt atccatcgtc gctgcaccgt tcagcaggct
    29281 tttccatgca ctgcggtaaa aatggccgct ggcgaaaacc atcaccgcca gggttatcag
    29341 gccgataacc aaccacaggc tgcggttgtc ggcggtgact atcatgttat cgccgatcat
    29401 cccccagacc atcaccggga tacccaccgc cagtgcgaca attgcctgcc agcggaagcg
    29461 cttcatcgta gcgacggcgg tttcttgctg gcgttcgcgg cgtttcgcgt catcttcaat
    29521 cgcttccgcg ccgtagcccg ctttttccac cgcctgcact aaatcttgtg gggaggcact
    29581 gcccatcacc agcgcagtac gctccgccag gtttacccgt gcctgagtga cgcccggtac
    29641 gctttgcagc gcattttgta cgcgggtgac acagctggcg cagctcatgc cgctcagcag
    29701 caactgctgg ctgtcatcgt catcggcggt cgctgccgga agcgcctcag aaaccgctgt
    29761 cagtgcttcc gacgggattg atgactccgc cagcggttta gcctttgggt ggcttacaga
    29821 tgcgtcataa cccgcttgtt tgatggtttc gatcagctgt tctgcactgg cagtcccggt
    29881 aacgtgcgct tcagtgatag acacatccgc ttgttccaca tccgggcgct gttcaagact
    29941 ttctttcacg cgtttaacgc agtgaccgca ggacaggccg tccaggttca ggtcgatagt
    30001 ttgtgacata aaacactcct ttaagacagt tttgactggc tgtgataaag gttaaacctt
    30061 ccagcaaggg gaaggtcaag aaattaataa accaggcggg taaaagtccg taaagattaa
    30121 aaaatcggct cgatttgcat caggattaga catttatttc tttgttttcc tgtagttaag
    30181 ttgcgggtgc taagttaaat caggatgcct gaaaatcggc actggggtga ggaattacct
    30241 cccgcatcta taaaaaggag ttaacaaaag atgttagatg caaacaaatt acagcaggca
    30301 gtggatcagg cttacaccca atttcactca cttaacggcg gacaaaatgc cgattacatt
    30361 ccctttctgg cgaatgtacc aggtcaactg gcggcagtgg ctatcgtgac ctgcgatggc
    30421 aacgtctata gtgcgggtga cagtgattac cgctttgcac tggaatccat ctctaaagtc
    30481 tgtacgttag cccttgcgtt agaagatgtc ggcccgcagg cggtacagga caaaattggc
    30541 gctgacccga ccggattgcc ctttaactca gttatcgcct tagagttgca tggcggcaaa
    30601 ccgctttcgc cactggtaaa tgctggcgct attgccacca ccagcctgat taacgctgaa
    30661 aacgccgaac aacgctggca gcgaatttta catatccaac agcaactggc tggtgagcag
    30721 gtagcgctct ctgacgaagt caaccagtcg gaacaaacaa ccaacttcca taatcgggcc
    30781 attgcctggc tgctgtactc cgccggatat ctctattgtg atgcaatgga agcctgtgac
    30841 gtgtacaccc gtcagtgctc tacgctcatc aatactgttg aactggcaac gcttggcgcg
    30901 acgctggcgg cgggtggtgt gaatccgttg acgcataaac gcgttcttca ggccgacaac
    30961 gtgccgtaca ttctggccga aatgatgatg gaagggctgt atggtcgctc cggtgactgg
    31021 gcgtatcgcg ttggtttacc gggcaaaagc ggtgtaggtg gcggtattct ggcggtcgtc
    31081 cctggcgtga tgggaattgc cgcgttctca ccaccgctgg acgaagaagg caatagtgtt
    31141 cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg gctataacgt gtttaagggc
    31201 tgatcatgat gaacacggaa ggtaataacg gtaacaaacc cctcggtcta tggaacgtcg
    31261 tttccatcgg tattggggca atggtggggg cggggatctt cgcgctgctg gggcaggctg
    31321 cgttgctaat ggaagcctcg acctggggcg cctttgcttt tggcggtatt gtggctatgt
    31381 tttccggtta tgcctatgcg cgtctggggg cgagctatcc cagcaatggc ggtattatcg
    31441 acttttttcg tcgcggatta ggcaacggcg tcttttcgct agcgctctcg ttgctatacc
    31501 tgttgacgct ggcggtgagc atcgccatgg ttgcccgtgc ttttggcgct tatgccgtgc
    31561 agtttttgca tgaaggcagc caggaggagc accttatttt gctctacgcg ttggggatca
    31621 ttgcggtgat gacgcttttc aactccttaa gcaaccatgc ggtagggcgg ctggaagtga
    31681 tcctcgtcgg cattaaaatg atgatcctgt tattgctgat tattgccggt gtctggtcgc
    31741 tgcaaccggc gcatatttcc gtctctgcgc cccccagctc cggtgcgttc ttctcctgta
    31801 ttgggataac cttccttgcc tatgcgggct ttggcatgat ggcgaacgcg gcggataaag
    31861 tgaaagatcc gcaggtcatt atgccacggg cgtttctggt ggcgattggc gttaccacgt
    31921 tgctttatat ctcgctggcg ctggttttgc ttagcgatgt atcggcatta gagttagaaa
    31981 aatatgctga taccgccgta gcgcaggctg cttctccgct gctcgggcat gtgggttatg
    32041 tgatcgtcgt catcggcgct ttactggcga cggcttcagg cattaacgcg aacctgttcg
    32101 ccgtgtttaa catcatggac aacatgggca gcgaacgcga actgccgaag ctaatgaata
    32161 aatccctgtg gcggcagagt acctggggca atatcatcgt cgtggtgttg ataatgctga
    32221 tgacggcggc gctaaattta gactcactcg ccagcgttgc cagcgccacc tttttgatct
    32281 gctatctggc ggtgtttgtg gtggcgatcc gcctgcgtca tgatattcac gcctcgttgc
    32341 cgattcttat cgttggtacg ttggtgatgt tgttggtgat cgttggcttt atctacagcc
    32401 tgtggtccca gggcagccgt gcgttgatat ggattattgg tgcaatctta ctcagcctta
    32461 ttgtggcaat ggtcatgaag cgtaataaaa ccgtataaca cctctctgtg cgcagtactt
    32521 cctgtattat tgtggtggcg gtcgatattc gcactggcaa aaaaacgtgc ttgaatatct
    32581 gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa cgatgaacat cagcgatgta
    32641 gcaaaaatta ccggcctgac cagcaaagcc attcgcttct atgaagagaa ggggctggtg
    32701 acgccgccga tgcgcagcga aaacggttat cgcacctaca cgcagcagca tctcaacgaa
    32761 ctgaccttac tgcgccaggc acggcaggtg ggctttaacc tggaagagag cggcgagctg
    32821 gtgaatctgt ttaacgaccc gcagcggcac agcgccgacg tcaaacggcg cacgctggag
    32881 aaggtggcgg agatcgaacg acacattgag gagctgcaat ccatgcgcga ccagctgctg
    32941 gcactggcga atgcctgccc tggcgatgac agcgccgact gcccgattat cgaaaatctc
    33001 tccggctgct gtcatcatcg ggcagggtga ttaagacgag acggctcgga tatgtagggt
    33061 tatcccttct atcgcaatga cttcaacatg cgtacctgca ccgagatcct cgctggcgct
    33121 gacaggccat gaactgtcac cgacgcgcat atgaccgcgc ccgttgacca gcggagattc
    33181 cagcacaaaa cgtcggccaa tcagctgctg cccgcgctgg tttaaatgac tatcgctgtg
    33241 cttttgttcg cgcacccgcc gcgacaacca tttccaccac agccaggcgg cgagcagcgt
    33301 caggatggca aacattaccc cttgccactc ccaacccagc ggcaccagcc agaccaccag
    33361 gccggtaatc accgctgcca cgccgctcca caacaaataa ccatttccgc ccagcatctc
    33421 ggctgccagc agcaacccgc cgagactgag ccagaaaata tgtggatgaa cgaccattaa
    33481 ctccatcatg gctgagtccg tttgttggcg ctgtctttca ccagctcggc aatcccggca
    33541 atcgacccca tcaggctgct ggcctctaat ggcatcatca ctactttgct gttactggag
    33601 gaaccgatct gctgtaacgc ttcggtgtat ttttgcgcta cgaagtagtt caccgcctga
    33661 atatcaccgg aggcgatagc ttcagacacc attttggtgg cgcgggcttc tgcttcggcg
    33721 gaacgttcac gcgcttcagc ctgtaaaaac gccgactgac gttcgccttc cgctttcagg
    33781 atttgcgact gtttttcacc ttcggctttg aggatttccg cctgacggat cccttccgct
    33841 tcaagaatgt aagcgcgttt ggtacgttcc gctttcatct gcgcgttcat tgaagagata
    33901 agctctgccg gtgggcgcac gtcgcgaatt tcaatacggg tgactttaat cccccacggg
    33961 ttggtggctt catcgacaat acgcagcagg cgtgagttga tgctgtcgcg ctgagagagc
    34021 atttcgtcaa gttccattga acccaacacg gtacggatgt tagtcatggt caggttgatg
    34081 atcgccagct ccagattgct gacttcatag gcagcgcgtg gcgcgtcaat cacctgaata
    34141 aaacagactg cgtcgatggt aacgttggcg ttatctttcg agataacttc ctgggaaggg
    34201 atatcgagca cttgctccat catattgatc ttgcgaccaa tgcgatccat aaacggcacc
    34261 accagactga gccccggctg taacgttttg gtatagcgac caaaacgttc taccgtccat
    34321 tgatagccct gcggcacgat tttgacgccc gcgccgacaa tgaccagcgc gacaaaaatg
    34381 agaatcggga taaagataag catcggaaaa acctcctgtt gtaccgtcca taatcagcaa
    34441 aattgctgct tgattaaaca aattatacct gattactgaa agagagttcc cccttattcc
    34501 tgcgaaggat aaactgtttt tagtaaaaat cagaaaaagg gaacagcgat gcaggaaaat
    34561 agtcctttgc ttcagctaca aaacgtagga tatctggcgg gtgatacgaa gattcttaat
    34621 aacatcaatt tttcgctgcg tgctggcgaa tttaagttaa ttaccggtcc ttctggttgt
    34681 ggcaaaagta cgctgctaaa aatagttgct tcattgatca gcccaaccag cggaacgtta
    34741 ctgtttgaag gtgaggatgt cagcacacta aaaccagaaa tctaccgcca gcaagtctct
    34801 tactgcgccc agacgccgac gctgtttggc gatacggtat acgataatct gatctttccc
    34861 tggcagatcc gtgaccagca gcctgacccg gccatttttc tcgattttct cgaacgcttc
    34921 gctttgccgg acagcatttt gacgaagaat atcgccgagc tatctggtgg tgaaaaacaa
    34981 cgcatctcat tgattcgtaa cctgcaattt atgccgaagg ttttattgct ggatgaaata
    35041 accagtgcgc tggatgaaag taataaacat aacgtcaatg agatgatcca tcgttatgtg
    35101 cgcgagcaaa atattgccgt gctgtgggtg acacacgata aagacgaaat taatcatgcg
    35161 gataaagtga ttacactgca accgcatgcc ggagaaatgc aggaagcacg ctatgaactc
    35221 gcataatatt actaacgaat cattagcact ggcattaatg ctggtggtgg tggcaatctt
    35281 aattagccat aaagaaaaac tggcgctgga gaaagatatt ctatggagcg tcgggcgagc
    35341 gataattcag ctgattattg tcggctatgt gctgaagtat attttcagcg tggatgatgc
    35401 cagcctgaca ttattgatgg tgttatttat ctgctttaat gcggcgtgga acgcgcaaaa
    35461 acgcagtaaa tatattgcta aagcttttat ctcatcgttt attgccatta cggtcggggc
    35521 gggaattacc ctggcggtgc tgattctctc aggggcgatt gaatttatcc cgatgcaggt
    35581 gatccctgtc gccgggatga ttgccggtaa cgccatggta gcggtggggt tgtgttacaa
    35641 caatttaggg caacgggtca ttagcgaaca gcaacagatt caggagaaac tgagtcttgg
    35701 cgcgacgccg aagcaggctt cagcgatatt gattcgcgac agtattcgcg cggctttaat
    35761 tccgacggtc gattcagcaa aaacggttgg cttagtgagt ttaccaggaa tgatgtccgg
    35821 gctgatattt gccgggattg atccggtgaa ggcgattaaa tatcagatta tggtgacctt
    35881 tatgctgctc tcaaccgcca gcttgtcgac cattattgcc tgctatttaa cctatcgtaa
    35941 gttttataat tcgcgccacc agttggtggt gactcaattg aagaagaaat gatgcgatgc
    36001 cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc cgaacaacgg aatatcaata
    36061 caacaatgca tacagctggc ggcgatactt cgacgccagt gcatcacccg tacccagcgc
    36121 agcgaggatc tcctggaacg ttttacgcgt ctgaccgtct gcggcggtga gatctttacg
    36181 cagatgcccg aacagcaact ccagcgcctc ttcattgcgc ccaacctgat gcagttgcag
    36241 cgccagttgc gtcgccagtg cggcatcttc tggattctcc gccacctgct gttgcaactg
    36301 ttgaatttcc ggcttatcag ccgcctgctt cagcagttcg atttgcgcta ccagcccctg
    36361 gtagcgggtg tcctgatcct gcaacggaat ggttttcagc accgcttccg catcttcaga
    36421 acggttcagc gcaatcagcg tttctgccag cagcaggccg atctccccgt tctgattcga
    36481 caactgccag gcgtctttca gcaatggcag ggcatcggtg taattgcctt cctgcatcag
    36541 ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc agcactttat ccagcagggc
    36601 gcggatcgcc tcttccggtt gcggcccctg gaagccatct accggttgcc cgttctggaa
    36661 cagatacacg gtcggaatcg cgcgcagacc aaactgggcg gcaatcatct gctccgcgtc
    36721 gcagtccagc ttcgccagaa taaattgccc gttgtactgc gccgcgaggc tttccaggat
    36781 cggggttaac tgcaaacagt gctggctacg ttcagaccaa aaatagaaca gcaccggagt
    36841 ggtcatcgac tgttcaagaa cctgttgcag gttagattcg ttaatgttga caatattttc
    36901 tacggacatg gagtcgctct ctgttgtcga tattttcttt gacatggggg cttaagcgcg
    36961 cgcttcaact cacccctgca atattttgtc catcacgcgc ccaggcagca ggcgctcgag
    37021 caccattact gcccaggtca ccagcgttac cggatagcgc atcttcggct tctcgctaat
    37081 aaaagcatgg cgcactttgt cgaccaccgc ttccggtccc aacgtaaagc gggcggcgat
    37141 gccgggattt tcgactggtt tatcactttg cgtctggttg acgttgtcgg tgaagcgagt
    37201 acgaatggga ccgggttcga tcaggctgac tttaattccg ctgtggcgta gctccatgcg
    37261 cagcgcgtct gaccacgcct ccagcgcata tttactggcc gcgtaagcgc cacgacccgg
    37321 cgtggagatt aatcccatca ccgatgatgt catcacaata cgcccttcac cgtgcggtaa
    37381 catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg aaaaagttgg cggaaaactg
    37441 ctgttccatc tgcgcacggc tgatggtgga aagggggcca tacatgccga atccggcatt
    37501 gttaaagatc ccatacagac aattatcggt cagggcgatc acctcgtctg ctgcgcgatt
    37561 aacactttct ggtgaatcca gatcgatcaa cacgccggta aatcccatgt tgttcatacg
    37621 ctcaacatca ttcggttttc ggcaacctgc cagcacatga aaaccctggc gttttaattc
    37681 gagcgcgctt tccaggccaa ttccactgga acatccggta attaagaccg atttttgcat
    37741 aactttacct gtcaggatct ccgttgcttt atgagtcatg atttactaaa ggctgcaact
    37801 gcttcgccat ccagtcggca ataaacggct gggcgtcgcg gttgggatga ataccgtcat
    37861 cctgcatcca ttgtggcttg aggtagacct cttccataaa aaagggcagc agcggaacat
    37921 caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc ttcattataa cggcgaccat
    37981 agtttgcagg cagacgtatt tgcattaaca atggttcagc gttggcggct ttgacatcct
    38041 gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg aaaaccacgc aaaccatcat
    38101 tgcgcccagt tcaaccagca cccaacgcgg ctgatgctgt ttgagcagag ccggaaggcg
    38161 cgccagccct tgttgcgagg tgtcgccgct gatgctggca ttaactaccg acgttttact
    38221 ctgccactta tcattcaaca aggcaggcca ggccgcgctg gcagacattc gatacccggc
    38281 gctcaggcta tcacccagaa tcaataacgt gtccgctgcg gcggcacgga aggttaacag
    38341 gaccaggaac aggaagggca aatgccagcg gaaaacattg ttgaagttca tcatcttaag
    38401 aagtccgtcg gtcaggggga gcatgaactc tccatcctca ccggagttga gctggttgtc
    38461 aaacgtggcg agaccatcgc actggtgggc gagtcgggat cgggtaagtc aaccttgctg
    38521 gcgatcctcg ccgggcttga tgacggcagc agtggcgaag tgagtctggt gggacaaccg
    38581 ctacataata tggacgaaga agcgcgggca aagttgcgcg cgaagcacgt cggctttgtt
    38641 tttcagttat ttatgttaat tcctaccctt aacgcgctgg aaaacgtcga gcttccggct
    38701 ctgctgcgcg gtgagagtag cgcggaaagt cgtaacgggg cgaaagcgtt gctcgaacag
    38761 ttagggctgg gtaaacgtct ggatcatctt ccggcacagc tttccggcgg tgaacagcaa
    38821 cgagtggcgc tggcacgagc ctttaatggt cgacctgatg tgctgtttgc cgacgaaccc
    38881 accggcaacc ttgaccgcca gacgggcgat aaaattgccg acctgctgtt ttccctcaac
    38941 cgtgaacatg gcaccacgtt gattatggtg acccacgacc tgcaactggc ggcacgctgc
    39001 gaccgctgct tacggctggt gaacgggcag ttgcaggagg aagcatgatt gcacgttggt
    39061 tctggcgcga atggcgttcg ccgtcgctat taattgtctg gctggcgcta agcctggcgg
    39121 tggcctgcgt gctggcgctg ggcaatatca gcgatcgcat ggagaagggc ttaagccagc
    39181 aaagccgtga gtttatggcg ggcgatcggg cgttgcgcag ttcacgcgaa gtgccgcaag
    39241 cgtggctgga ggaagcgcaa aagcgcggcc tgaaagtcgg caagcagctg actttcgcca
    39301 caatgacctt tgcaggcgac acaccgcagc tggcgaacgt caaagcggtg gatgatatct
    39361 acccgatgta tggcggtctg caaactaatc cccctggcct gaaaccgcag gcgggcagcg
    39421 tattgctggc cccacgcctg atggcactgc ttaacctgaa aacgggcgac accattgacg
    39481 tgggcgatgc caccttgcgg attgccggag aagtgattca ggaaccggat tccggtttta
    39541 accccttcca gatggctccg cgtctgatga tgaatctggc ggatgtcgat aaaaccggag
    39601 ccgtgcagcc ggggagtcgg gtcacctggc gttataaatt cggcggcaac gagaaccagc
    39661 tcgacggcta tgagaaatgg ttgttacctc agcttaaacc cgaacaacgc tggtacggtc
    39721 tggaacagga cgaaggcgcg ctggggcgat cgatggaacg ctcgcaacag ttcctgctgc
    39781 tttcggcgct tctgaccttg ctgctggcag tggcagcggt ggcggtagcg atgaatcatt
    39841 actgtcgcag tcgctacgat ctggtggcga tcctcaaaac gctgggggca gggcgagcgc
    39901 aactgcgtaa gctaatcgtc ggtcagtggt tgatggtgct gacgctttca gccgttaccg
    39961 gtggggccat aggcctgttg ttcgaaaacg tgttgatggt gctgctcaag cccgttctgc
    40021 ctgctgcact accgccagcc agcctctggc cgtggctgtg ggcgcttggc accatgacgg
    40081 tcatctcgct gctggtgggg ctacgaccat accgcttgtt gctggcaacg cagcctttac
    40141 gcgtattacg taatgatgtg gtcgcgaacg tctggccgct gaagttttat ctgccgattg
    40201 tcagtgtggt ggttgtgctg ctgctcgccg gattaatggg tggcagcatg ctgctttggg
    40261 cggtgctggc gggcgcggta gtactggctt tgctgtgcgg tgtgctgggc tggatgctgc
    40321 tgaatgtact tcgccgcatg acgctgaaat cgctgcctct gcgcctggcg gttagccgcc
    40381 tgttacgtca gccgtggtca acgttaagcc agctttcggc attttcgctc tcctttatgc
    40441 tgctggcact gctgctggtg ctgcgtggcg atctgctcga ccgctggcag cagcagctac
    40501 cgccagaaag cccgaactac tttttaatta acatcgccac agaacaggta gcaccgctaa
    40561 aagcgttcct cgcggaacat catatagtcc cggaatcatt ttatccggta gtgcgggcgc
    40621 ggctgacggc gattaatgat aagccgacag aaggtaatga agatgaggcg cttaatcgcg
    40681 aactcaatct tacctggcaa aatacgcggc ccgatcataa tccgattgtc gccggtaact
    40741 ggccgccaaa agccgatgaa gtgtcgatgg aagaggggct ggcgaaacgc ttaaacgttg
    40801 ccctcggtga taccgtgact tttatgggcg atacccagga gttccgcgct aaagtgacca
    40861 gcctgcgcaa agtggactgg gaaagtctgc ggcctaattt ctattttatt ttccctgaag
    40921 gggcattaga cgggcaaccg cagagctggc ttaccagttt ccgctgggag aatggcaacg
    40981 gcatgttgac gcaactcaac cgccagttcc cgaccattag cctgttagac attggcgcga
    41041 ttttaaaaca ggtcggtcag gtgctggagc aggtaagtcg ggcgctggag gtgatggtgg
    41101 tgctggtcac cgcctgcggt atgttgctgt tgctggcaca ggtgcaggtg ggaatgcgcc
    41161 agcgtcatca ggagctggtg gtatggcgca cactcggtgc ggggaaaaaa ctgctgcgca
    41221 cgacattgtg gtgtgatgtt cgccatgctt gggtttgttt ccggcctggt ggccgcaatt
    41281 ggtgcggaaa cggcactggc agtgttgcag gcgaaagtgt ttgatttccc gtgggagcca
    41341 gactggcgat tgtggattgt tctgccgtgc agcggtgcgc tgctgctgtc gcttttcggc
    41401 ggctggctgg gtgcgcgact ggttaagggt aaggcgctgt tcaggcagtt tgtggggtga
    41461 tgacaggtgt tagattgcct gatgcgctac gcttatcagg cctacgaaga gcgtgcaata
    41521 tattgatttt gcaagattgt gtaggccgga taaggcgttt acgccgcatc tggcatgaac
    41581 agtgtgcatt ttggcaataa atatgaaacc aactgtcatg tcggtttttt taaaggccga
    41641 aggaggtaaa ccttcggcct tagctacgtt tcctcagtta ttcaataaac tgacggatca
    41701 gttccagcag cgcgattagc gccttaagaa cgatgagaac tttatcgaga aaagttttca
    41761 tccgttgctc ccttaaggga gaaagacaat cacagcttac ccttacgttg cctataacta
    41821 tgattttctt catgtgctag atgaggactg tctctctcca aattagagca ccagaatcag
    41881 caccacctgt atcctgacca ggacttaaaa gtacttttcc gcactgcggt aatcccttga
    41941 ggcagtatca ctactagcgt cgggattgta tcgccaacat agctgtttga atatgtaggc
    42001 atcctgactt ataattcact tgctagatga ggactgttta atttctgccc ggaaccacca
    42061 agtcttgggc ggaaaaaata aaaaaaccga ctgtcatgtc ggttttttta tatctgtatt
    42121 ctggtgcaac cattttgttc tcacgacaat cgccgtttca gaataccgcc catcactgcg
    42181 ccttaaccca taccgccact tccgcccact caccgcgaaa gacaactttt tccgcttttt
    42241 tctcaagctg atagcgatac attgggtcgt aatattcttt aagtaacggc accagccagg
    42301 ccagatgacc gtcggtgctg ccggtggtga gttgcgttgt cagcgccgta tccagctggg
    42361 cagcgagttc gttatagcgc tgtaacccca gccgacgctt aatcgccgaa agtccgtgat
    42421 gcaggtattc gcaatattcc tgccagccct gttcgtcgcc ataagcgtgg gtaaaatcat
    42481 gatgcatacg caagaaatac tcttcattca ggcgttcaag acggatctca aacggatctt
    42541 ctaccaccgc aatcgccgcc tgagtcattc gctcgcgcag acattccggc aggtgattcg
    42601 aaccgatcat ccggctttca tcttccagca cccacaggcg caaatcctga cgggcgtcgg
    42661 tttttagcat ttcggcagcc agcaggtttt caaaactcgc ctggctaagt tgtggttgta
    42721 acgtgcgacc aaacgccgaa ccgcgatgac gcgccagccc ttccagatca acaccgtttg
    42781 gctgttgttg cactaatagc gttttaccgc tgccggtaca accgccaatc agcactatcg
    42841 gtttttgtgc cagttcaatg gtcgcctgaa tcgcggtctg gcgcagtgcc ttataaccgc
    42901 cttccaccag cggataatca atccccgctt catgcagcca gcgttgcaca atatgtgaac
    42961 gttgaccgcc acgggcgcag cagagaatac cttgcggatt ttgcaggcac gctgcccgcc
    43021 aggcgtacat acgctgctga cgaatttcac ccgccaccag tttatgcccc agcgccagcg
    43081 ctgcgtctga gccttgctgt ttatagcagg tgccaacgga ggcgcgttca tcgttatcca
    43141 ttaacggcag attgatagcg gcgggcattg cgccttgctc aaactcgata ggggcgcgaa
    43201 catcaataat gggcgtatca gcaatcagca gggcacgata gtcctgttcc gtgtgtctct
    43261 cttgcatagt taaaagtgaa cctcaaatca gcttgcgcgc tattttacgc gccaacgcgc
    43321 aaggaaactt gatttttaac tgcgtggggt gccgaaaatt tctaaaaatc cgctgatttc
    43381 cggcctgtgt tgggtaaaca aggtcacaat atcttctacc gctttgcagc tgccaaattt
    43441 gcgccatgcc agactcaatg gcgaaggagg gcgcatcgtt gggattaccc ggctgaccag
    43501 ttgttgatta tcgatcattg actggcaaag tgattttggc aaaaaaccaa tgccaacacc
    43561 cgccagatgg gcggcgattt tcgtttccat atcgggaaca ataatctctt tttgccccgg
    43621 caatcgccag gcgacgcgtt tggttaaggt gccggcgctg tcttcaatat tgaccgccgg
    43681 aaagcgacgc aactgcgctt ctgttagcgg ctcttcaacg ttcgccagcg gatgatccgc
    43741 cgccatgaca aatcgccatt gcaccgatcc taagggatca agactaaagg tatttgccag
    43801 cgcctcagtt cccgtgaccc cgatagccag cgaaaaacct tcgtacaata gcgagtccca
    43861 gacgcccata tagatttgtc gggagatgtg aaactgggta aaggggtaac gctcattcag
    43921 ccacgccagc aactgggcga cggcctgggg gttatagagc aggttgttga tgacaatatt
    43981 cacctggcgt tccacgccat cattcacctg ttgcagctcg ctgggcatac tttccagcca
    44041 gctcagccag tctctggcct gggaaagtag atgctcgcca gccgctgtca acgttacgct
    44101 acgagtcgta cggaaaaaca gcgctactcc ggtattctct tccagaagtt taatgcgata
    44161 actgatcgtc gccgtggttt tacataatcg ttctgccgct tttgaaaaac ttcctgtttc
    44221 agcaaccgca atgaaagtcc gcaaggtttc tggatcgaac atcttcaggt atcccctttt
    44281 aaatccgcaa gttgcgtgat tttcttatcc tctgatttat cagtattttt acatgataat
    44341 cctgttcaat ttgtggacta aatctagttt tggaaaaaca ttccaacttt tgtattaatg
    44401 ttgttctctt aaggttttag attgcctgtt attgaaacca agctgaccgg tcggcggtgg
    44461 ttgaacggaa ttatgttaca aggacaaaaa gatgaaactt caggtattac cgttaagtca
    44521 ggaagccttt agtgcttatg gcgacgtaat cgaaacgcag caacgggatt ttttccatat
    44581 taacaatggc ctggtggagc gttaccacga tttggcgctg gttgagattc ttgagcaaga
    44641 ccgtacgctt atcagcatta accgcgcgca accggcgaat ctgccgctga ccattcacga
    44701 actcgaacgt catccgctgg gcattcaggc ctttatccca atgaaaggtg aagtgtttgt
    44761 ggtggtcgtg gcgttaggcg acgacaaacc agacctgtca acgctgcggg cgtttatcac
    44821 caacggtgaa cagggggtga attaccatcg taacgtctgg catcacccac ttttcgcctg
    44881 gcagcgcgtc accgattttc tgaccatcga tcgcggcggc agtgacaact gtgatgttga
    44941 aagtattcct gaacaggaac tctgttttgc gtgacgcctg caaccgactt gcataagata
    45001 aactaattgt tcattgttta tgctcacttg taggtcggag ttaacgtagg tatgacggaa
    45061 gttagacggc gcggcaggcc aggacaggcg gagcctgtgg cacagaaggg cgcacaggcg
    45121 ttagagcggg gaattgcgat tctgcaatat ctggaaaaaa gtgggggaag ttcgtcggtt
    45181 agcgatattt ctctcaatct ggatttgccg ctctccacga cctttcgctt gctgaaggtt
    45241 ttacaagcag cggattttgt ctatcaggac agtcaattag gctggtggca tataggatta
    45301 ggtgtcttta acgtcggtgc ggcgtacatc cataaccgcg atgtcctctc cgtcgccggg
    45361 ccgtttatgc gccgcctgat gttactttcc ggcgaaacgg tcaatgtcgc gatccgtaac
    45421 ggcaatgaag cggtattaat tggtcagtta gagtgtaaat cgatggtcag gatgtgtgcg
    45481 ccactgggca gtcgtctgcc gctgcatgct tccggtgcgg gcaaagcgct gctttatccg
    45541 ctggcggaag aggagttgat gagcatcatt ctgcaaaccg gtttgcagca gtttacgcca
    45601 actacgcttg tggatatgcc caccttgctg aaggacctgg aacaagcgcg tgaactgggc
    45661 tataccgtag ataaagaaga gcatgttgta ggtctgaatt gcatagcttc agcaatttac
    45721 gatgatgtcg gtagagttgt tgccgctatc tccatctccg ggccttcatc aagactgaca
    45781 gaagatcgtt ttgtcagtca gggtgagctg gtcagagaca ccgcccgcga tatcagcacg
    45841 gcgttgggac tgaaagcaca tccatagtgt ctgtcgcatc ccgctctgcg gagcgggttt
    45901 ttttgacaaa atttgaaagt tggaaaattt ttccaactaa tagaggtagg aataaaatgg
    45961 caaaaatgag agccgttgac gcggcaatgt atgtgctgga gaaagaaggt atcaccaccg
    46021 ccttcggcgt tccgggagct gcaattaatc cgttctactc agcgatgcgt aagcacggcg
    46081 gtattcgtca cattctggcg cgtcatgtgg aaggtgtttc gcacatggcg gaaggttata
    46141 cccgcgcaac ggcagggaat atcggcgtat gtctggggac ttccggtcct gcgggcacgg
    46201 acatgatcac cgcgctctat tccgcttctg ctgattccat tcctattctg tgcattaccg
    46261 gccaggcacc gcgcgcccgt ctgcataaag aagattttca ggccgtagat attgaagcaa
    46321 ttgctaaacc ggtcagcaaa atggcggtta cagttcgtga agcggcgctg gtgcctcgcg
    46381 tgctgcaaca ggcatttcac ctgatgcgtt ctggtcgtcc gggtccggta ctggtggatt
    46441 taccgttcga cgttcaggtt gcggaaatcg agtttgatcc tgacatgtac gaaccgctgc
    46501 cggtctacaa acctgctgcc agccgtatgc agatcgaaaa agctgtagaa atgttaatcc
    46561 aggccgaacg tccggtgatt gttgccgggg gcggggtaat caatgctgac gcagctgcac
    46621 tgttacaaca gtttgctgaa ctgaccagcg ttccggtgat cccaacgctg atgggctggg
    46681 gctgtatccc ggacgatcat gaactgatgg ccgggatggt cggtctgcaa accgcgcatc
    46741 gttacggtaa cgcaacgttg ctggcgtccg acatggtgtt tggtatcggt aaccgttttg
    46801 ctaaccgtca taccggttcg gtagagaaat acaccgaagg gcgcaaaatc gttcatattg
    46861 atattgagcc gacgcaaatt ggtcgcgtgc tgtgtccgga tctcggcatt gtctctgatg
    46921 ctaaagcggc gctgacactg ctggttgaag tggcgcagga gatgcaaaaa gcgggtcgtc
    46981 tgccgtgtcg taaagaatgg gtcgccgact gccagcagcg taaacgcact ttgctgcgca
    47041 aaacccactt cgacaacgtg ccggtgaaac cgcagcgcgt gtatgaagag atgaacaaag
    47101 cctttggtcg cgatgtttgt tatgtcacca ctattggtct gtcacaaatc gctgcggcac
    47161 aaatgctgca tgtctttaaa gaccgccact ggatcaactg tggtcaggct ggtccgttag
    47221 gctggacgat tccggcggcg ctggggggtt gtgccgctga tccgaaacgc aatgtggtgg
    47281 cgatttctgg cgactttgac ttccagttcc tgattgaaga gttagccgtt ggcgcgcagt
    47341 tcaacattcc gtacatccat gtgctggtga ataacgccta tctcggcctg attcgtcagt
    47401 cacaacgcgc ttttgacatg gactactgcg tgcaactcgc tttcgagaat atcaactcca
    47461 gtgaagtgaa tggctacggt gttgaccacg taaaagtagc ggaaggttta ggttgtaaag
    47521 ctattcgggt cttcaaaccg gaagatattg cgccagcctt tgaacaggcg aaagccttaa
    47581 tggcgcaata tcgggtaccg gtagtcgtgg aagttattct cgagcgtgtg accaatattt
    47641 cgatgggcag cgaactggat aacgtcatgg aatttgaaga tatcgccgat aacgcagcgg
    47701 atgcaccgac tgaaacctgc ttcatgcact atgaataagg gagataaatg atgttacgtt
    47761 tctctgctaa tttatcgatg ttatttggtg aatatgattt tctcgcccgt tttgagaaag
    47821 ctgcgcagtg tggttttcgc ggcgttgaat ttatgtttcc ttatgactac gacattgaag
    47881 aattaaaaca ggtactggcg agtaataaac tcgaacatac gctgcacaat ttaccggcag
    47941 gtgactgggc ggcgggcgag cgcggtattg cctgtattcc tggccgtgaa gaagagtttc
    48001 gggatggcgt agcagcagcg att
//
LOCUS       DAZSIO010000012        46314 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZSIO010000012 DAZSIO010000000
VERSION     DAZSIO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.012"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(21..1880)
                     /gene="gss"
                     /locus_tag="ACHX5T_000578"
     CDS             complement(21..1880)
                     /gene="gss"
                     /locus_tag="ACHX5T_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR8465905.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2085..2951
                     /gene="yghU"
                     /locus_tag="ACHX5T_000579"
     CDS             2085..2951
                     /gene="yghU"
                     /locus_tag="ACHX5T_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR8465906.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(2980..3061)
                     /locus_tag="ACHX5T_000580"
                     /pseudo
     CDS             complement(2980..3061)
                     /locus_tag="ACHX5T_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3074..3322)
                     /gene="hybG"
                     /locus_tag="ACHX5T_000581"
     CDS             complement(3074..3322)
                     /gene="hybG"
                     /locus_tag="ACHX5T_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR8465907.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3456..3797)
                     /gene="hypA"
                     /locus_tag="ACHX5T_000582"
     CDS             complement(3456..3797)
                     /gene="hypA"
                     /locus_tag="ACHX5T_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR8465908.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3790..4278)
                     /gene="hybE"
                     /locus_tag="ACHX5T_000583"
     CDS             complement(3790..4278)
                     /gene="hybE"
                     /locus_tag="ACHX5T_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR8465909.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(4271..4765)
                     /locus_tag="ACHX5T_000584"
     CDS             complement(4271..4765)
                     /locus_tag="ACHX5T_000584"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR8465910.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4765..6468)
                     /gene="hybC"
                     /locus_tag="ACHX5T_000585"
     CDS             complement(4765..6468)
                     /gene="hybC"
                     /locus_tag="ACHX5T_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR8465911.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6465..7643)
                     /gene="hybB"
                     /locus_tag="ACHX5T_000586"
     CDS             complement(6465..7643)
                     /gene="hybB"
                     /locus_tag="ACHX5T_000586"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR8465912.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7633..8619)
                     /gene="hybA"
                     /locus_tag="ACHX5T_000587"
     CDS             complement(7633..8619)
                     /gene="hybA"
                     /locus_tag="ACHX5T_000587"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR8465913.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8622..9740)
                     /gene="hybO"
                     /locus_tag="ACHX5T_000588"
     CDS             complement(8622..9740)
                     /gene="hybO"
                     /locus_tag="ACHX5T_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR8465914.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9929..10216)
                     /gene="yghW"
                     /locus_tag="ACHX5T_000589"
     CDS             complement(9929..10216)
                     /gene="yghW"
                     /locus_tag="ACHX5T_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR8465915.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10335..11222)
                     /gene="yghX"
                     /locus_tag="ACHX5T_000590"
     CDS             complement(10335..11222)
                     /gene="yghX"
                     /locus_tag="ACHX5T_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR8465916.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11379..12419
                     /gene="gpr"
                     /locus_tag="ACHX5T_000591"
     CDS             11379..12419
                     /gene="gpr"
                     /locus_tag="ACHX5T_000591"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR8465917.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12459..12953)
                     /locus_tag="ACHX5T_000592"
     CDS             complement(12459..12953)
                     /locus_tag="ACHX5T_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR8465918.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13144..14028
                     /gene="yghA"
                     /locus_tag="ACHX5T_000593"
     CDS             13144..14028
                     /gene="yghA"
                     /locus_tag="ACHX5T_000593"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR8465919.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14300..14725)
                     /gene="exbD"
                     /locus_tag="ACHX5T_000594"
     CDS             complement(14300..14725)
                     /gene="exbD"
                     /locus_tag="ACHX5T_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR8465920.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14732..15466)
                     /gene="exbB"
                     /locus_tag="ACHX5T_000595"
     CDS             complement(14732..15466)
                     /gene="exbB"
                     /locus_tag="ACHX5T_000595"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR8465921.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15718..16905
                     /gene="metC"
                     /locus_tag="ACHX5T_000596"
     CDS             15718..16905
                     /gene="metC"
                     /locus_tag="ACHX5T_000596"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR8465922.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            17045..17704
                     /gene="yghB"
                     /locus_tag="ACHX5T_000597"
     CDS             17045..17704
                     /gene="yghB"
                     /locus_tag="ACHX5T_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR8465923.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17744..18643)
                     /gene="yqhC"
                     /locus_tag="ACHX5T_000598"
     CDS             complement(17744..18643)
                     /gene="yqhC"
                     /locus_tag="ACHX5T_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR8465924.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18837..20000
                     /gene="yqhD"
                     /locus_tag="ACHX5T_000599"
     CDS             18837..20000
                     /gene="yqhD"
                     /locus_tag="ACHX5T_000599"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR8465925.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20105..20932
                     /gene="dkgA"
                     /locus_tag="ACHX5T_000600"
     CDS             20105..20932
                     /gene="dkgA"
                     /locus_tag="ACHX5T_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR8465926.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            21132..22058
                     /locus_tag="ACHX5T_000601"
     CDS             21132..22058
                     /locus_tag="ACHX5T_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR8465927.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            22109..22366
                     /gene="yqhH"
                     /locus_tag="ACHX5T_000602"
     CDS             22109..22366
                     /gene="yqhH"
                     /locus_tag="ACHX5T_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR8465928.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22409..24628)
                     /locus_tag="ACHX5T_000603"
     CDS             complement(22409..24628)
                     /locus_tag="ACHX5T_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR8465929.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24739..26151)
                     /gene="ftsP"
                     /locus_tag="ACHX5T_000604"
     CDS             complement(24739..26151)
                     /gene="ftsP"
                     /locus_tag="ACHX5T_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR8465930.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26226..26963)
                     /gene="plsC"
                     /locus_tag="ACHX5T_000605"
                     /gene_synonym="parF"
     CDS             complement(26226..26963)
                     /gene="plsC"
                     /locus_tag="ACHX5T_000605"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR8465931.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27197..29455)
                     /gene="parC"
                     /locus_tag="ACHX5T_000606"
     CDS             complement(27197..29455)
                     /gene="parC"
                     /locus_tag="ACHX5T_000606"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR8465932.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(30001..30483)
                     /locus_tag="ACHX5T_000607"
     CDS             complement(30001..30483)
                     /locus_tag="ACHX5T_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR8465933.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30536..30928)
                     /gene="ygiW"
                     /locus_tag="ACHX5T_000608"
     CDS             complement(30536..30928)
                     /gene="ygiW"
                     /locus_tag="ACHX5T_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR8465934.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            31080..31739
                     /gene="qseB"
                     /locus_tag="ACHX5T_000609"
     CDS             31080..31739
                     /gene="qseB"
                     /locus_tag="ACHX5T_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR8465935.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31736..33010
                     /gene="qseC"
                     /locus_tag="ACHX5T_000610"
     CDS             31736..33010
                     /gene="qseC"
                     /locus_tag="ACHX5T_000610"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR8465936.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(33056..33388)
                     /locus_tag="ACHX5T_000611"
     CDS             complement(33056..33388)
                     /locus_tag="ACHX5T_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFR8465937.1"
                     /translation="MLKQKIKTIFEALPYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            33707..34288
                     /gene="mdaB"
                     /locus_tag="ACHX5T_000612"
     CDS             33707..34288
                     /gene="mdaB"
                     /locus_tag="ACHX5T_000612"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR8465938.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            34319..34633
                     /locus_tag="ACHX5T_000613"
     CDS             34319..34633
                     /locus_tag="ACHX5T_000613"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR8465939.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(34681..36573)
                     /gene="parE"
                     /locus_tag="ACHX5T_000614"
     CDS             complement(34681..36573)
                     /gene="parE"
                     /locus_tag="ACHX5T_000614"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR8465940.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(36602..37183)
                     /gene="yqiA"
                     /locus_tag="ACHX5T_000615"
     CDS             complement(36602..37183)
                     /gene="yqiA"
                     /locus_tag="ACHX5T_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR8465941.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(37183..38010)
                     /gene="cpdA"
                     /locus_tag="ACHX5T_000616"
     CDS             complement(37183..38010)
                     /gene="cpdA"
                     /locus_tag="ACHX5T_000616"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR8465942.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(38035..38457)
                     /locus_tag="ACHX5T_000617"
     CDS             complement(38035..38457)
                     /locus_tag="ACHX5T_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR8465943.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(38458..39087)
                     /gene="nudF"
                     /locus_tag="ACHX5T_000618"
     CDS             complement(38458..39087)
                     /gene="nudF"
                     /locus_tag="ACHX5T_000618"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR8465944.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            39292..40773
                     /gene="tolC"
                     /locus_tag="ACHX5T_000619"
     CDS             39292..40773
                     /gene="tolC"
                     /locus_tag="ACHX5T_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR8465945.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            40921..41592
                     /locus_tag="ACHX5T_000620"
     CDS             40921..41592
                     /locus_tag="ACHX5T_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR8465946.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            41598..42758
                     /locus_tag="ACHX5T_000621"
     CDS             41598..42758
                     /locus_tag="ACHX5T_000621"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR8465947.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(42796..43584)
                     /gene="ygiD"
                     /locus_tag="ACHX5T_000622"
     CDS             complement(42796..43584)
                     /gene="ygiD"
                     /locus_tag="ACHX5T_000622"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR8465948.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            43727..44500
                     /gene="zupT"
                     /locus_tag="ACHX5T_000623"
     CDS             43727..44500
                     /gene="zupT"
                     /locus_tag="ACHX5T_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR8465949.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(44558..44728)
                     /gene="yqiD"
                     /locus_tag="ACHX5T_000624"
     CDS             complement(44558..44728)
                     /gene="yqiD"
                     /locus_tag="ACHX5T_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR8465950.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(44989..45642)
                     /gene="ribB"
                     /locus_tag="ACHX5T_000625"
     CDS             complement(44989..45642)
                     /gene="ribB"
                     /locus_tag="ACHX5T_000625"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR8465951.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(45746..45894)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            46016..46306
                     /gene="ubiK"
                     /locus_tag="ACHX5T_000626"
     CDS             46016..46306
                     /gene="ubiK"
                     /locus_tag="ACHX5T_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR8465952.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        11498 a        12389 c        11564 g        10863 t
ORIGIN      
        1 cggggttgct acaacatgta ttactttttc accacaatta acggttcaat atcactctct
       61 tttttgatga ccagtgattc atcaccgcgc aaacacgtcc caccgtagtt gccgccaacg
      121 gtgaaggtac atacctgaat gtatttaccg tccactttcg gcaaacacca cagttgctga
      181 tagatgtttt tctgctcggc aaatttaccg ctggttttgt ccagcacctc ttcatgatgg
      241 ctgaccaggt cgatattgct accacagcga ccggcgatcg gtttcactgc gtaacctgtt
      301 ttcaccaatt catcattaac agtgaaatcg gtatccagca ggtaacgatg gtgcgggaac
      361 agcgaccaga ggatcggcag aatcgctttg ttgccgggga tcaccgtcca cagcggctca
      421 aagaccagca cttccgggcg cagcaatacg tcgataagac gcacttcgtt ttgcggatga
      481 ccggtacgga ttggcaccgc agcaaactca cggtcgctaa cttcacgaat ctgatcaaac
      541 gcggtttccc acgcccaggt tttccacacg cagttaacca gtcgcccttc cccatcaatc
      601 agttgcccgg cagcatccca gcccagttca tccagcccgc gcaagatacg cgtttcaaag
      661 cccgcctggt gcagcgcctg ctccataaac tgcgcgtgat agttttcctc gatatctttg
      721 tcctgcatga tatggacaaa cggacgtgca cgactgtgtt tccaggcacc agccagttcg
      781 ttaatcagcc cttccgccgg attgaagccg ttgcctttat agccctgctc cgcccaacgt
      841 tcgaggatca agcccgcttc ggtatgacag gaggcggagt cggcgttgta ttcgtaaacc
      901 ttcaggccac gctcatccat gcagaaatcc atacgaccag tgatcatatg gtgacggcga
      961 cgctgccagg agagacgcaa acgtggccag aggattttcg gaatgtcgaa cagcgccagc
     1021 aggttgtcat ctttcagcac cttgtcggtt gcgtgaagat acatcagatg cagctcgttg
     1081 gtggctttaa ttagctcctg ctcggcactc tcggtaatgg tgtagtaatg atacggatcc
     1141 tgattgatca cctgaccgtt ggcctgcaca taggcgtttt gtagcggatc tttttcatcc
     1201 agccatttac cgtcaaactg gcctttgttt tccagacgcg ctccactgat tttcagcagc
     1261 tcgcctgcaa tttccggctg cggtaagctg tattcagtat cttccgtctg gatcatccag
     1321 cccagaatgg tggtgtcatc aaaagtgtct ttaagggtat agcagccgtt ttcgaccacc
     1381 atctccagct cgcgcgtcca ctgttgccct tgcggcaacg gggaatgaat cacgttctgt
     1441 tccgcaatac ggactttgtt gccatgcaat tgggtaatga tggcgacatg gccagtgtct
     1501 ttaaattcac cgcctttatc ccagataaga agcgcacccg cgaccggcgc acgcggcgag
     1561 ccgttaggaa atgcctgcaa tggcaggatg ttgtcattaa ccacttctcg caggaagcgc
     1621 agcgagaaaa tctcccacgc catacccacg tcagtaaaga ccacaccgta attcagaaag
     1681 agaaaacggc gagcaaattc aacgcattgc cacttgtggc ccatatattc gtcgtcgata
     1741 tagctacgga atacggcgtc atcttcgtat tcctgcggat cgagagaact gtaatctgaa
     1801 gagtagattg ctaccccacc tggggcgtag cccaataatg tcccgaacgg ggcatcctgg
     1861 ctggtcgttc ctttgctcat cactttacct ttaacaatac aacctaagca gttggcgtaa
     1921 ttgttgtgct ctgattacct gctcagcgac attaaccgga cagaggtcag actaatcata
     1981 cacctcatcg cagcggctgg cgcaaagggt taaaaaattc acattctgta cagcaagtga
     2041 cctgctacac tgcttcaaca ctaccactca gaaggcaact cactatgaca gacaatactt
     2101 atcagcccgc gaaagtctgg acgtgggata aatccgctgg cggcgcgttc gccaatatca
     2161 atcgcccgtt ttctggtccg acgcatgaaa aaacgctgcc cgttggcaaa catccattgc
     2221 aactttattc gctgggaacg ccgaacggtc agaaagtaac gattatgctt gaggagctac
     2281 tggcgctggg cgttactggt gcagagtacg acgcctggct gattcgtatt ggcgatggcg
     2341 atcaattctc cagcggcttt gtcgaagtga acccaaactc gaagatcccg gcgctgcgcg
     2401 atcatacgca taatccgccg atccgcgtgt ttgaatctgg ttcgatcctg ctttatctgg
     2461 cggagaaatt tggctacttc ctgccgcagg atctggcaaa acggactgaa acgttgaact
     2521 ggctgttctg gttacagggt gccgcgccgt tcctcggcgg tggttttggt cacttttacc
     2581 attacgcgcc ggtgaaaatt gagtacgcca tcaaccgctt taccatggaa gccaagcgcc
     2641 tgctcgacgt gctggataag caactggcgc agcataagtt tgttgcgggc gatgagtaca
     2701 ccattgcgga tatggcgatt tggccgtggt ttggcaacgt ggtgctcggc ggcgtgtatg
     2761 atgccgcaga gtttctggat gcgggaagtt ataaacatgt gcaacgctgg gcgaaagaag
     2821 tgggcgaacg tccggcagtg aaacgtgggc gtattgttaa ccgcaccaac gggccgctga
     2881 acgagcagtt gcatgagcgc catgacgcca gtgattttga gacaaataca gaagataagc
     2941 gtcaggggta agggttggtg ttcgtcgcag caagccatcc aggccggata aggcgttcac
     3001 gccgcatccg gcaatcgtgc acaatgcctg atgcgacgct gccgcgtctt atcaggccta
     3061 caaaagctaa tcatcacgcg ctggtaatgt cgtaatccat ttggcgcagt gcgtctaatg
     3121 tggctttggc ttcatcttcg tcgatgatgc tcatggcaaa tccgacgtgt accagcaccc
     3181 actggcccag cagatcggca gggttacctt cgcaaatcag ggcgatattc acgtcgcgct
     3241 tgataccaca tacttcaacc tgcgcaagct ggtgaatatc ttcaccgaca gccagcacct
     3301 ggcctggaac gccaatacac attgttaact ccggttattt cgattgccgg acaaacgcga
     3361 aaaagaacct ttagcgggtg ttccctctca ccctaaccct ctccccggag gggcgagggg
     3421 accgtccgtg cgctttttag gcgttgtcct cagtcttatt caacttcaat acttttgacg
     3481 atcagcgaat cgccggtatc gacacgcaac cgctcgccgt gacagagcgg acactgcgca
     3541 tcgtgctgat gaatctccac cacctggctg caatcccagc accaggcctg ggcgggttta
     3601 tagacgatat gtaaatcgca cccttgcgcc accgttccgt ggcagacaat ttcaaaacta
     3661 aaacggacgg cgctctcctc aacgcaggag agcgcgccaa tttccagcca cacggcggtg
     3721 acgcgcttaa catcgtgctg ctccgcctgc cgttggataa tttcaacggc gctctggcaa
     3781 agcgacaact catgcatttt cgcccctcct gcggccaaac agcagggcgc gacgccctgc
     3841 gtgtggtaca tccggattgg tgactggcaa cgaaaggatc attcgtgcgc agtcatccgt
     3901 caggcgttgg ccttcttcaa tcgacatgct gtgcgaaagc ggcgacatca gcgaacagga
     3961 gaggtattgc gaaacaccgt ccagttcgcc aacggtaaag gtcatggtgc catacggcag
     4021 ttgcagacca attttttcac tcactttgcg cagcggccag agttgatccg ggccggggaa
     4081 gataactgca ctcagcatcc acggggtgat cacacacccc gtccactgac cttcgaacag
     4141 cgtaaaatca gaaacataca ccggcattga aggatgcaga aaagagagat cgtgcatcga
     4201 acgccgggca atttcttcaa acgctgcctg tacttgcgcc ttcggggagg tctggaaacc
     4261 tgctatctct tcagtcatga atcgcctccc gtgggatggc ttccacgcct gattcgcgca
     4321 gcgcagccag aacctgctca agcgcaggtt caatcattgc ttcaaccgtc ggcgttaagc
     4381 cgatgtgtgg ctccagcgat tccgggatca cgccgaccag ggtcagcttt ttcggaaact
     4441 caccggtgaa gcgcagggcc gacaatacgt cggccaggcc aagctgatgc ggagagattt
     4501 tgttggtaaa caacgccgga acttcttcat cccgcaggat catcatcgtt cccggagtgt
     4561 tctttttcga cacaatggca tccgcaataa tcagatgatc gcgatttgcc atgtcgccaa
     4621 gcagctccat tcccgccgtg ccgccatcga ggatctcaac ataatccggc agaatgtatc
     4681 gttgctctaa cgcttcgaca atccgcacgc cgatggcttc atcggtcagc aaaatattgc
     4741 cgacccctaa gactaaaata cgcattacag aaccttcact gaaaccactt cgttgccgtc
     4801 ggcatccact acgtgtaccg cacaggccat gcacgggtcg aaggagtgaa tggtacgcac
     4861 cacttccagc ggtttattcg gatcggcaac cggtgtaccc accagcgact gctcgtaagg
     4921 accgacgtca tcattgaagt tacgcggacc agagttccag gttgatggaa caaccgcctg
     4981 gtagttgctg atgataccgt ctttaataac catccagtga gagagcatac cgcgcggcgc
     5041 ttcgaggaag ccaacacctt tgaactcacc cgttgccgga atgttcggct tcacaaaggt
     5101 ggtgtgatcg cctttgccga tattggtgat cagtgcactg tattggtttt gcaggatatc
     5161 ctgcaattca cagcagtgaa cggtacgacc aataatacgg cccagcgtgg agtgcagctg
     5221 tgccacttcc agcgtgttgc cagtcagttt ctgataaatc gcaacgattt cattcagttt
     5281 gttctgggta gattcgcgac ctgccgccag tttcaccagc atattagcca gcggacccac
     5341 ttctaccgtt ttgccgtaga aagtcggtga tttcacccag gaatatttcc cgtcgtcaga
     5401 ccaaccatca taagccggaa tggtggtacc ttcccacggc gcctgtggcg cttcgtcttt
     5461 ataccaggag tgcttcgcgc tttcctgaat gcctttgatc aggtattcat cggaatgaga
     5521 agtgatcgga cgatacgagg acagatccgc attctcaatg tagcctcccg ggaacaggaa
     5581 gctgccgttt ttgctgtcgg tcgggaattc cggcacgctc aggtagttca ccgcaccttt
     5641 accgcgcgtc agccattccg ggtagaacgc ggcgataacc gcggtatcaa ccttataaac
     5701 ctgctcaaca aagtcgctca gtttgtcgat gaaagacttg atgtacatca ggcgctcaag
     5761 gttcaacacg cccaacccgt cgaggttgat tgggttcgcg acaccaccca cagccaggtt
     5821 ctgaatgtgc ggcgttttac cgcccagcag cgccacgacg cggttagcgt cacgctggca
     5881 ctccagcgcc tgcaggtagt gcgctaccgc aatcaggttc acttccggcg gcagtttcat
     5941 cgccgggtga ccccagtagc cattggcgaa aatacccaac tgaccgctgg caaccagatc
     6001 tttgatcttg ttctgaactt tagtgaactc ttccggactg ttcagatgcc aggtcgaaac
     6061 gcctttcagc atttcggagg ctttggttgg gtcagcttgc agtgcagaag tgatgtccac
     6121 ccagtccagc gccgaaagct gatagaaatg aacgatatgg tcatgcgtgg tgtgcgcagc
     6181 cagaatgatg ttacggatgt attgcgcgtt aaccggaacg tcgatattca gcgcactttc
     6241 tgccgcacga acggaagaca gcgcgtgagt ggtagtacat acgccacaga tacgttgcac
     6301 aatcatccat gcatcgcgcg gatcgcggtt tttcacgatc tcttccatgc cgcgccacat
     6361 ggtaccggaa gcccatgctt tcgaaacgac gccattttcg atttcgcaat cgatgcgtaa
     6421 atgcccctca atacgggtta ccggatcaat agtaattctc tggctcatgc tttgctcgcc
     6481 tcatgacgat tatgatcgtt ttgttttaaa ggaggaagta tcggcagtag acgaatgagt
     6541 acgatgtaag cgcaaatctc aatagccaca aaaccaatag aaatcaacag ttcttcccag
     6601 gtcgggaagt aggcgtaacc gccgcccggg ttgaatgcca ccaacgaata ggtcagacgc
     6661 caggttgcac aacctaacag tgcgctcagt gctgacagga acagcatacg ggaatcatta
     6721 cgcagcttcg ccacacgcag aacgaccagc gggaagagca tcagcaggac ttcaatccag
     6781 aacatcacgg agtagaagtc accggcaaac gctaacgaca gcttgtcgcg atagataagc
     6841 tcgccaaagc gcagcccgat gaaaatcgcc agcaacacac tgatggtgtt ggtcagcttg
     6901 acgaacaggc tcttttcatc cggaccgttg ccacgcagac ccgcctgcac cagtgaacct
     6961 tcaaagatga caatcgagaa gcccatgatg aacgccgtca gcagcgagaa cagcggcaac
     7021 atttcatagc tctgccagag cggatgcacc ttgtagcccg ccgagatcat cagcgacccc
     7081 attgaagact ggtgcatggt cggcagcagc gcaccgagcg cgatgatgaa gaacatcacc
     7141 ttgttcagac gttgtagcga caccttccag cccagacgtt caaacagtgc cggagcaaac
     7201 tccagagcca tcacgccgat gtagatggtc atacagaccg ccgtctcgaa cagtaccgag
     7261 ttcacgttga agtgacccgg aatgtagaag tacggcaggt tccagtagcg acccacgtcg
     7321 atagtgatcg acaagccacc cagtgagtaa ccaaacagac tcgccaacag cgccggacgc
     7381 accagcggat ggtattgccc acggttaaag acgtataccg cccacgccag cgcccagccg
     7441 ccacaggcaa agccggtgcc aatcagcagg tcaaacgcga tccacacgcc ccacgggaag
     7501 ccgccgttca ggtcagagac agagcccaga ccgaacacca gacgcttcac aatcaggagc
     7561 atacagatga cgattaacgg tccaaaaatc atgaccggtt tactgatgat tttgccgccc
     7621 agcggttgtg gatcatgact catgatcgtc tcctccgtcg tgatggtcgt ttttggtgtt
     7681 gcgacgaacc agcacggtta agcccgccag cacagccagt ggtagcatca tgcctttata
     7741 cagggtgtgt tgaatatttt cggaacgcgc accggtagaa agatcgtcca gtttcggcag
     7801 gtcgagattt tcataaggca cacccgtcag taccagaacc tgagtaccgc cgccctcttt
     7861 ctcgccgtac agatgcggat aatatttcgg caccgtatgc aggtaagtgt cgccagattt
     7921 cagcgtctga cgcggatagt ggtattcgct gccaggcttc agcgccagac gttttttcgc
     7981 ctccgccatc agctcttcac gcgtaccgaa aatcaccgcg cccgccgggc acacttctac
     8041 gcagccaggc agaccgcctt tatcgagacg ttccacacct ttctggttgc acagctcgca
     8101 cttatgcagc gcaccaaacg ggttgttgta gtcgtacttc ggcacgttgt acggacaggc
     8161 gaccatgcag taacggcaac cggtgcacac gtctttgtcg taatggacaa tgccggtttt
     8221 cggatctttt ttcagtgcag agaccgggca cacagagaca cagttcggat cgacgcagtg
     8281 catacactgt ttcttaatgt acgcgtagcc gttctcctcc tggtctttgt tgacccctgt
     8341 gccgctggtc cacacctgaa tgatgttatt ggtatacggc gacagtttgt cgttgttcga
     8401 ccaggtctgt tccccttgcg ggttacgttc agggaagttg atatcctgac acttggtgac
     8461 gcaagcctgg cagcctacgc acaaggtcga gtcgtacaac atccccagcg atcccggaat
     8521 tggcgggcgg ttttcagcag ccgcatgact gacagacggc agcgcgcccg tcagcaatgc
     8581 cccgcaggag gctgctttaa taaaattacg tctgttcacg gttattctcc ccgtgagtca
     8641 gcgttatctt tcttttgctg acgacccagt tcacgcaccg ccatcacgct gacaccggca
     8701 accagcccaa ccacaccgcc gagcaaacca atagcgcctg cagagacgtt gccgccctct
     8761 ttagcgttaa catccggttt ctgtgaacgc ggagtctgat tttcgacgtt ggcaagctga
     8821 tggatgcctt tatggaagcc gatacctttt tcgttacagc catagcacgg gtgaccaatc
     8881 gccaccggcc acacaccgcc aacatcgcag aattgcagcg ttgagcagtt gccgtaagtt
     8941 tctggccctt tacagccgag gtggtacaag caccagcctt cgcggtggcc ttcatcaccg
     9001 aactctttgg caaaacgacc agcatcgaag tgcgggcgac gttcgcagtg ttcgtgaatc
     9061 agacggccat aggcgaaggt cggacggttt ttgtcatcca gtttcggcgg tttgccgtaa
     9121 gtgatgatgt gcgcaacggt cgcgaggaag ttgtgcgggt tcggcgggca gcccggaata
     9181 ttgataacgg ttttgcctgg cagaacttct tgcaagctga ctgcgccagt tgggttaact
     9241 ccagctgcgg caacaccgcc ccacgcggag caggaaccaa tagcgataat ggctgctgag
     9301 ccttccgccg ctttgcggat gtgatccaca atcggctcac cggcaaccat gcaataaata
     9361 ccgttatctt ttaatgggat ggaaccatcc accactaaca catactgccc tttgtacttc
     9421 tcgagagcgt tatgtttgtt ctcttcgacc tgatgaccga aggcggcgga aagcacttcg
     9481 tgatattcca gagagatagt ctccagcacg aggttttcta ccgttggatg cgttgcacga
     9541 agcagagatt ccgtacaacc ggtgcactcc tgcgcgccaa tccagataac tggcggacgc
     9601 tgcgggttag taaccgattc ggccatctct gcagcggctt tgctacttaa ccccatggtg
     9661 gcggctaatg ctgcacaaag cttcatgaaa tcacgacggt taatgccgtg agaatggatg
     9721 agggtgttat ctccagtcat ttatagttat tccgttgcga gaacctggca tatattttgc
     9781 ctcaatcgca aaatcaataa tgcgatcgat gcgccattta ccacacattt attatggtta
     9841 tcgatatcat gatactgcgg cggggcgcta aaacgaaggg aagaagcatt agtgtaatta
     9901 attaaagcaa gataatacgt atgtttgatc aattttcgag gcgataaccg gccataaaaa
     9961 agcgcaacgt agaacaggaa ttattctcac gaaagaaatc cattaccatc tctttatcca
    10021 gtccatagcg gcgcgtcaga atacccgctt cccaggcgct aagctggcga tctccggtct
    10081 tttcgtacat cctgtgtgag tagcctaata caaaaccgcg tttataatcg gcacagaatt
    10141 ttgtcacatt aaccgcactg tcggcatgcg ttgcttttaa tcccgccatt aagcctttac
    10201 caaaatggtt attcatcgtt cacctcattc attatatatc gccatatata acgatatttt
    10261 aggcaaagaa cgacttttgc gcacgctatt ttttcatact tgtttaagac gtaatcccgt
    10321 aagagataaa cctatcagga gagatattta tcgaaccatt tcagtgtcct ttgccaggca
    10381 agatcggcgg cagatttgtc ataacggggc gtggaatcat tatggaatcc gtgattaacc
    10441 cccggataga tataagcctc ataaacctta ttattgtctt tcaacgccgc ctcgtaagga
    10501 ggccagccct cgttgattcg ggtgtccagt tccgcgaagt ggagtagtaa aggcgcttca
    10561 atcttcgcca catcggcagt gggtgcctga cgaccataaa acggcaccgc gcaggccagt
    10621 tccggatacg cgacagccgc cgcgttcgat acgccaccgc catagcaaaa tccggtaata
    10681 cccactttac ctgtcgcttg cggatagcgt tgcataaact caattgcggc aaagaaatca
    10741 ttcatcagtt tggttggatc aacctgctgt tgcagctcac gacctttatc atcatttccc
    10801 ggataacctc caacggaact taatccgtca ggtgccaggg cgatataccc cgccttcgcc
    10861 actcgccgtg ccacatcttc gatatacgga ttcagtccac gattctcatg caccaccccc
    10921 acggctggcg ttttgccgct catttttgcg ggtttcacca gataaccccg tacctcgccg
    10981 tgaccatttg gcgaaggata cgtgatgtac tcagcaacaa tctccgggtc ggtaaactct
    11041 acctgagtcg ccagcgcata atttggcttg agcaaatcaa acaacgctaa tgccgtcatc
    11101 ccgcccaccg catacttcgc cgcaaggttg aggaactcac gtttcgaaat tttcccgtga
    11161 acgtaatagt cgtagtaatc caacaactct tgtgggaaat ctttggcggt taaacgcggc
    11221 atcgttgcat tcctcaactg gtgttttttt aagcaaagca taagcgcgtg tttttgccca
    11281 gtttttcgtc actctgtgag ccagactacg ggatacgcgc tggcgaatcg ctaaactaga
    11341 aacattgttt cgaaattgaa cggtggaaag gagaggtcat ggtctggtta gcgaatcccg
    11401 aacgttacgg gcagatgcag taccgctatt gcggaaaaag tggtttacgc ctgcccgcat
    11461 tatcgctcgg tttatggcac aatttcggtc acgttaacgc gctggaatca cagcgtgcaa
    11521 tcctgcgtaa agcgtttgat ttaggcatta cgcactttga tttagccaac aattacgggc
    11581 cgcctccagg aagcgcagaa gagaactttg gtcgcctgct gcgcgaggat tttgctgctt
    11641 atcgcgatga actgattatc tctaccaagg ctggctacga tatgtggccc ggcccgtacg
    11701 gctctggcgg ttcacgtaaa tacctgctcg ccagcctcga ccaaagcctg aagcgtatgg
    11761 ggcttgagta tgtcgatatc ttttactctc atcgcgtcga tgaaaatacg ccgatggaag
    11821 aaaccgcctc tgcgctggct catgcggtac aaagcggtaa ggcgctgtat gtcgggatct
    11881 cctcttactc gccagagcgg acgcaaaaaa tggtcgagtt gctgcacgag tggaaaattc
    11941 cgcttttaat tcatcaacct tcgtacaatt tactgaaccg ctgggtggat aaaagcggcc
    12001 tgctggatac cctgcaaaat aacggcgtgg gctgtattgc ctttactcct ctggctcagg
    12061 gattgctgac cggaaaatat ctcaacggca ttccgcaaga ttcacggatg catcgtgaag
    12121 ggaataaagt tcgtggtctg acgccgaaaa tgcttaccga agccaacctc aacagcctgc
    12181 gcttattgaa tgaaatggca cagcagcgtg gacaatcaat ggcgcaaatg gcgttaagct
    12241 ggttgctgaa agatgatcgc gtgacgtcgg tattgattgg tgccagccgc gcggagcaac
    12301 tagaggagaa cgtgcaggcg ctgaataatc tgacatttag caccgaggag ctggcgcaga
    12361 ttgatcagca tatcgccgat ggcgagctga atctgtggca ggcgtcgtcc gataaatgac
    12421 ctgttaataa cgggcgcgcg aaccgcgccc gaatcagatc agtgattatg acgagtcagt
    12481 cggtcaagat agcccatcac aaatgcagaa agcacaaacg tcagatggat aatgacgtac
    12541 cacatcagtt tgttatccgg gacatttttc gcatccataa agacgcgcag taagtgaatg
    12601 gaagaaattg ccacaatcga cgctgctact ttgtttttca gcgacgttgc gtccattttc
    12661 cccagccact tcagcttctc tttgttctcg gagatatcca gctgtgagac gaaattctca
    12721 taaccggaaa acatcaccat caccagcaaa ccgccaacca gcgtcatatc caccagcgac
    12781 agcaacacga ggatcaaatc tgattccgcc atcgagaaga tattcggcag tacgtgaata
    12841 atctcctgga agaacttcag cgccagggca actaacgcca gcgaaaggcc aaagtacacg
    12901 ggggcaagca gccagcgaga agcatacatt gcattttcaa gaaaacgttc catacattcc
    12961 tgtcgtgtag ttaaaacggg caacagtata tcgcaattga gtaaacagca cgcaacagca
    13021 aatcaacata tacgttaaac ctttgtcgca taattctgag gtttagggat cttcctggtt
    13081 cccttatcaa cgcatcgaga gcgtctaccc ttaaaggacg ttgaacctga agggagaaaa
    13141 acgatgtctc atttaaaaga cccgaccacg cagtattaca ctggtgaata tcccaaacag
    13201 aaacaaccga cgccaggcat ccaggcgaag atgacaccga taccggattg cggcgagaaa
    13261 acctatgttg gtagcggtcg cctgaaagat cgtaaagcac tggtgacagg gggcgattcc
    13321 ggaataggtc gcgctgccgc catcgcttac gcgcgtgaag gggctgacgt ggcgatcagt
    13381 tatcttcccg tggaagaaga agacgctcag gatgtgaaaa agatcattga agaatgcgga
    13441 cgcaaagccg ttctgctgcc aggcgattta agcgatgaga aattcgcccg ttcgctggtt
    13501 cacgaagcgc acaaggcgtt aggcgggctg gatattatgg cgctggtcgc cgggaaacag
    13561 gttgccattc cggatattgc agacctcacc agcgaacagt ttcaaaagac ctttgccatt
    13621 aacgttttcg cgctgttctg gctaacccag gaagcgatcc ccctgctacc gaaaggtgca
    13681 agtgtcatca ccacttcgtc aatccaggca taccagccaa gtccgcattt actggactat
    13741 gcggctacga aggcggcgat tctgaactac agccgtggcc tggcaaaaca ggtcgcggag
    13801 aaaggtattc gggtgaatat tgtcgcgcca ggccctatct ggacggcact gcaaatttcc
    13861 ggcggacaaa cgcaggataa gatcccgcag tttggtcaga aaacgccgat gaagcgtgcg
    13921 gggcaaccgg cggaactggc ccctgtatat gtttatctgg caagtcagga gtcgagctac
    13981 gtcaccgcag aagtgcacgg cgtgtgcggc ggcgagcatt taggttaaaa aaatgcccgg
    14041 ttgtgaaaag caaccgggca tcattgtgaa ttcactctta cctgatgtgg cgaaaacgcc
    14101 ttatccgcct acaaacgcat gaagattcaa catgttgcaa tagattttgt aggcctgata
    14161 agcgtagcgc atcaggcatt gagcacctaa tgccggatgc gtcgtgaacg ccttatccgg
    14221 cctacaaacg cacgaagatt caacatgttg caatagattt tgtaggcctg atgagcgtag
    14281 cgcatcaggc aattctactt tacttcgctt tggcggtttc ttcgccgacc agacctatct
    14341 tcaggtaacc cgcctgatgc agcgtatcca ttaccttcat caacgtctcg taatcgacgg
    14401 ttttgtccgc tcggaagaag atggtggtgt ctttcttgcc ttcggttaac gcattcaacg
    14461 ccgtaatcat tgtttcatcg gtgaccggat cgttaccgat aaacatcgag ttgtctgcct
    14521 tcaccgacag ataaaccggt ttttccgggc gcggctgcgg cgtgctggta gaagcaggca
    14581 agttcacctt cacatctacc gtcgctaacg gtgccgccac cataaagata atcagcagaa
    14641 ccaacatcac gtcgataaac ggcgtcacgt tgatatcatg catttcgccg ttatcgtcga
    14701 ggttttcgtt aagatgcatt gccatcggac attatcctgc gcgtaatttt tgtgcgacac
    14761 gaaccggatg cgcagcggcg ctggcttcca gatctaggtc acggctttgc agcaacaata
    14821 cctgcgctgc aacatcaccc agcatcgctt taaagccgcc aatctggcgt gcaaatacgt
    14881 tatagataac gaccgcagga atcgctgcca cgaggccgat tgccgttgct aacagagctt
    14941 ctgcgatacc cggcgcaacg actgccaggt tagtggtctg cgtttgcgcg ataccaataa
    15001 agctgttcat gatgccccag accgtaccaa acagaccaac gaacggagaa atcgcgccga
    15061 tggttgccag atagccgtta ccgcggccca tttgacgacc cactgcggcg acccgacgct
    15121 ccagacggaa gctggtacgt tctttaatac cttcgttatc gtcgctgcct tctgacagtt
    15181 ccagctcatt ctgcgcttca ttgagcaaat gcaggcttaa gcttttgcta ccaaaatcag
    15241 cggcgatatc gttggcctgg tttaaggaac gtgcttcagc cagcagttgc tgctcgcgct
    15301 taaggcgacg cttctgattg aagaactcta cgctcttact gaagaagatt gcccaggtga
    15361 ctacagaggc caaaataagc ccaatcatca cgcacttaac gacaatatcg gcgtactgat
    15421 acatacccca gacggaaagg tccgtctgca ttaaattatt acccactgtg tatctccagg
    15481 acgcaagtca caaaatctgc gcataataat atcaaaacga cgtcgaattg atagtcgttc
    15541 tcattactat ttgcatactg ccgtaccttt gctttctttt ccttgcgttt acgcagtaaa
    15601 aaagtcacca gcacgccatt tgcgaaaatt ttctgcttta tgccaattct tcaggatgcg
    15661 cccgcgaata ttcatgctag tttagacatc cagacgtata aaaacaggaa tcccgacatg
    15721 gcggacaaaa agcttgatac tcaactggtg aatgcaggac gcagcaaaaa atacactctt
    15781 ggcgcggtaa atagcgtgat tcagcgcgct tcttcgctgg tatttgagag tatggaagcc
    15841 aaaaagcacg cgacgcgcaa tcgcgccaat ggcgagttgt tctatggacg gcgcggaacg
    15901 ttaacccatt tttccttaca acaagcgatg tgtgaactgg aaggtggcgc aggctgcgcg
    15961 ctatttccct gcggggcggc agcggttgct aattcgattc ttgcttttgt cgaacagggc
    16021 gatcatgtgt tgatgaccaa caccgcctat gaaccgagtc aggatttctg tagcaaaatc
    16081 ctcagcaaac tgggcgtaac gacatcgtgg tttgatccgc tgattggtgc cgatatcgtt
    16141 aagcatctgc agccaaacac taaaatcgtg tttctggaat cgccgggttc catcactatg
    16201 gaagtccacg acgttccggc gattgttgcc gccgtgcgca gtgtggtgcc ggatgccatc
    16261 attatgatcg acaacacctg ggcagccggt gtgctgttta aggcgctgga ttttggcatc
    16321 gatgtttcta ttcaggccgc caccaaatat ctggttgggc attcagatgc gatgattggc
    16381 actgccgtgt gcaatgcccg ttgctgggag cagctacggg aaaatgccta tctgatgggc
    16441 cagatggtcg atgccgatac cgcctatata accagccgtg gcctgcgcac attaggtgtg
    16501 cgtttgcgtc aacatcatga aagcagtctg aaagtggccg aatggctggc agaacatccg
    16561 caagttgcgc gagttaacca ccctgccctg cctggcagta aaggacacga attctggaaa
    16621 cgagacttta caggcagcag cgggctattt tcctttgtgc ttaagaaaaa actcaatgat
    16681 gaagagctgg cgaactatct ggataacttc agtttattca gcatggccta ctcgtggggc
    16741 gggtatgaat cgttgatcct ggcaaatcaa ccagaacata tcgccgccat tcgcccacaa
    16801 ggcgagatcg attttagcgg gaccttgatt cgcctgcata ttggtctgga agatgtcgac
    16861 gatctgattg ccgatctgga cgccggtttt gcacggattg tataacattg ccacttttgg
    16921 acaattttgc agacatttta ttgtgaaaag tcttaaattg ttgcgtccgg gatcaaggcg
    16981 tcccggacga ttcaggagta caataggcag ataaaggctt aaacgctgtt ccacaggaaa
    17041 gtccatggct gttattcaag atatcatcgc tgcgctctgg caacacgact ttgccgcgct
    17101 ggcggatcct catattgtta gcgttgttta ctttgtcatg tttgccacgc tgtttttaga
    17161 aaacggcctg ctgcccgcct catttttgcc aggcgacagc ttgttgatac tggcaggcgc
    17221 attgattgcc caaggggtta tggattttct gcctacgatt gcgattctga ccgccgcagc
    17281 aagtctgggc tgctggctaa gttatattca ggggagctgg ttagggaata ccaaaacggt
    17341 gaaaggctgg ctggcacagc ttcctgctaa atatcaccag cgcgccacct gcatgtttga
    17401 ccgccacggt ctgctggcgc tgctggctgg acgttttctt gcatttgtcc gtacgctgct
    17461 gccaaccatg gcgggaattt ccggtctgcc aaaccgccgc ttccagtttt tcaactggtt
    17521 aagtggattg ctgtgggtca gcgtggtaac cagttttggc tatgccttaa gtatgattcc
    17581 gttcgttaaa cgccatgaag atcaggtaat gacgttcctg atgatcctgc caattgcctt
    17641 gttaaccgct ggcttgttag gcacgctgtt tgtggtgatt aaaaaaaaat actgtaacgc
    17701 ctgacgattt tccccgttcc cggctgctgt accgggaacg tatttaattc ccctgcatcg
    17761 cccgcattct tgccgcatct tcccccggcg tcacaccgaa gtaacgttta aactcacggc
    17821 taaattgcga tgcgctttca tagccgacgc gcatcgctgc tgcgctggcc ttcatgccgt
    17881 catggatgat catcatccgc gccttatgca gacggtaatt cttcaaatac tgcaacggcg
    17941 aggtgctggt gacagactta aaattatggt ggaacgccga tacgctcatg ttggcttctg
    18001 ccgccagttg ctcgacgctc aggttttcgg tgtatttatt ctcaattcgt ttcagcacgc
    18061 ggctaatcag actgaagtga gtctggcgac tgaccagcgc cagtaacgcg ccgccgcaag
    18121 gtccggtcag cacgtagtac agaatttcgc ggatgatctg tttgccgaga atacgcgcat
    18181 ccagtggtcg ctccatcacg tcgagtaacc gctccgccgc gcataaaatc tcttctgata
    18241 acgtggcgga gttaatcccg ctggctgcca tcgacggctg gaaatgctca tcttcgccaa
    18301 tgtccatcaa cagttcctgt aactgcaaaa tatcgacatt gagacgcaac cctgccagcg
    18361 gcacctctga cgtggcatag gtttcgcact caaacggcaa cggcaccgtc agcagcaggt
    18421 attcattggc atcataacga aacacgcgtt cattgatata accgatttta tgcccggaaa
    18481 agagaattat gatgccaggc tcgtacatca ccggtgtacg tgcgaaaggc gtctcgccat
    18541 acaacaaacg cacatcgggc aacagttctg acaaactatt ttctttattt ttcagtttat
    18601 taactttatc cgccagcaag cgacaaatct cttcacgttt catatcgcgt aatttcttag
    18661 gaataatgcg gcaatttgat tgtgcgcaat tttgtagcat ttctccagca ctctggagaa
    18721 ataggcaaga cattggcaga aatgagcatt gagagccagg gcgctggcga tcacaatgaa
    18781 aaacatcagg cagatcgttc tctgccctca tattggccca gcaaagggag caagtaatga
    18841 acaactttaa tctgcacacc ccaacccgca ttctgtttgg taaaggcgca atcgctggtt
    18901 tacgcgaaca aattcctcac gatgctcgcg tattgattac ctacggtggc ggcagcgtga
    18961 aaaaaaccgg cgttctcgat caagttctgg atgccctgaa aggcatggac gtgctggaat
    19021 ttggcggtat tgagccaaac ccggcttatg aaacgctgat gaacgccgtg aaactggttc
    19081 gcgaacagaa agtgactttc ctgctggcgg ttggcggcgg ttctgtactg gacggcacca
    19141 aatttatcgc cgcagcggct aactatccgg aaaatatcga tccgtggcac attctgcaaa
    19201 cgggcggtaa agagattaaa agcgccatcc cgatgggctg tgtgctgacg ctgccagcaa
    19261 ccggttcaga atccaacgca ggcgcggtga tctcccgtaa aaccacaggc gacaagcagg
    19321 cgttccattc tgcacatgtt cagccggtat ttgccgtgct cgatccggtt tatacctaca
    19381 ccctgccgcc gcgtcaggtg gctaacggcg tagtggacgc ctttgtacac accgtggaac
    19441 agtatgttac caaaccggtt gatgccaaaa ttcaggaccg tttcgcagaa ggcattttgc
    19501 tgacgctaat cgaagatggt ccgaaagccc tgaaagagcc agaaaactac gatgtgcgcg
    19561 ccaacgtcat gtgggcggcg actcaggcgc tgaacggttt gattggcact ggcgtaccgc
    19621 aggactgggc aacgcatatg ctgggccacg aactgactgc gatgcacggt ctggatcacg
    19681 cgcaaacact ggctatcgtc ctgcctgcac tgtggaatga aaaacgcgag accaagcgcg
    19741 ctaagctgct gcaatatgct gaacgcgtct ggaacatcac tgaaggttcc gacgatgagc
    19801 gtattgacgc cgcgattgcc gcaacccgca atttctttga gcaattaggc gtgccgaccc
    19861 acctctccga ctacggtctg gacggcagct ccatcccggc tttgctgaaa aaactggaag
    19921 agcacggcat gacccaactg ggcgaaaatc atgacattac gttggatgtc agccgccgta
    19981 tatacgaagc cgcccgctaa gctttttacg cctcaaactt tcgttttcgg gcatttcgtc
    20041 cagacttaag ttcacaacac ctcaccggag cctgctccgg tgagttcata taaaggagga
    20101 acgtatggct aatccaaccg ttattaagct acaggatggc aatgtcatgc cccagctggg
    20161 actgggcgtc tggcaagcaa gtaatgagga agtaatcacc gccattcaaa aagcgttaga
    20221 agtgggttat cgctcgattg ataccgccgc ggcctacaag aacgaagaag gtgtcggcaa
    20281 agccctgaaa aatgcctcag tcaacagaga agaactgttc atcaccacta agctgtggaa
    20341 cgacgaccac aagcgccccc gcgaagccct gctcgacagc ctgaaaaaac tccagcttga
    20401 ttatatcgac ctctacttaa tgcactggcc cgttcccgct atcgaccatt atgtcgaagc
    20461 atggaaaggc atgatcgaat tgcaaaaaga gggattaatc aaaagcatcg gcgtgtgcaa
    20521 cttccagatc catcacctgc aacgcctgat tgatgaaact ggcgtgacgc ctgtgataaa
    20581 ccagatcgaa cttcatccgc tgatgcaaca acgccagcta cacgcttgga acgcgacaca
    20641 caaaatccag accgaatcct ggagcccatt agcgcaagga gggaaaggcg ttttcgatca
    20701 gaaagtcatt cgcgatctgg cagataaata cggcaaaacc ccggcgcaga ttgttatccg
    20761 ctggcatctg gatagcggcc tggtggtgat cccgaaatcg gtcacacctt cacgtattgc
    20821 cgaaaacttt gatgtctggg atttccgtct cgacaaagac gaactcggca aaattgcaaa
    20881 actcgatcag ggcaagcgtc tcggtcccga tcctgaccag ttcggcggct aacgtgcaaa
    20941 ttctcccggt ggcggtaatg ttccgctacc ggacttttca gaaatcattt attcccctcg
    21001 cgtcccgccc gttgttactc ttccttgttc aggaatgcca aatataagga catcatcatg
    21061 cagagccgga agctcttaaa agaacaactc atctatatcc gggataaacg caacggagag
    21121 gtgaaaaaca gatgaaaata atacttctgt ttttagcagc cctggcaagt tttaccgtac
    21181 acgcgcagcc tccttcacag accgtagaac aaacagtccg gcagatttat cagaactata
    21241 aatcagatgc cagtacgcct tatttcggtg aaaccggaga gcgggcgata acttcagcgc
    21301 gtattcaaca ggcgcttacc ctgaacgaca atcttacact accgggcaat attggctggc
    21361 tggattatga tccggtttgt gattgtcagg attttggcga tctggtgtta gaaagcgtag
    21421 cgataaccca aactgacgtc gatcatgccg atgccgttgt gcgctttcgt atctttaaag
    21481 atgataaaga aaagaccaca cagacactga aaatggtggc ggaaaatggt cgttgggtca
    21541 ttgatgatat tgtcagcaat catggcagcg tcttacaagc agttaatagc gagaatgaaa
    21601 aaacgctggc cgctttagct tcgttgcaga aagaacagcc agaatccttt gttgctgaac
    21661 tctttgaaca tattgctgat tatagctggc cgtggacgtg ggtggtttcc gactcttacc
    21721 gccaggcggt taatgccttc tataaaacca ccttcaagac ggccaataat cccgatgaag
    21781 atatgcaaat agaacggcaa tttatttacg acaatccgat ctgttttggc gaagagtcgc
    21841 tattttcacg cgttgatgaa attcgagtcc tggagaaaac cgccgattcc gcccgcattc
    21901 atgttcgttt tacgctgacc aatggcaaca atgaagagca agagctggtt ttacagcggc
    21961 gcgaaggtaa gtgggaaatc gctgatttta tccgcccgaa cagcggcagc ctacttaagc
    22021 agattgaggc aaaaaccgcc gccagattaa agcaatgagc tgaattaaat aacaattagc
    22081 cggaacaata aataaaaggg aacactatat gaaaacgatt ttcaccgtgg gagctgttgt
    22141 tctggcaacc tgcttgctca gtggctgcgt caatgagcaa aaggtcaatc aactggcgag
    22201 caatgtacaa acattaaatg ccaaaatcgc ccggcttgag caggatatga aagcactacg
    22261 cccacaaatc tatgctgcca aatccgaagc taacagagcc aatacgcgtc ttgatgcaca
    22321 ggactatttt gattgcctgc gctgcttgcg tatgtacgca gaatgataaa aaaatccccg
    22381 gcagcatgtc agttgccggg gatttttttt aacgtccaac cgccgcttta gggcgtttct
    22441 tcgcaccagc attcaccgga cgagattgcg tagacgacgc tttttttgcc gtagcaggcg
    22501 tctgacgctg agtcgccatc ggcgtatgtt tagtcaacgc cggacgggta ttgcggttct
    22561 ggcgacgagc ttcacgcatc tcttcaatgg ttggcgcagg cactaagcaa tcgcgacggc
    22621 tgccaatcag atgctttttg cccatcgctt ccagcgcctg gcggattaac ggccagtttg
    22681 ccggatcgtg gtaacgcaac aacgctttat gcaaacgacg ctgtttgtcg cccttcggta
    22741 cgaagacgcc ttcgctctta taaccaatct tcgccagcgg ttttttcccg gtgtaataca
    22801 tggtggttga gttcgccagc ggcgatgggt agaagttctg tacctggtcg agacggaaac
    22861 gatgcttttt cagccacagc gccaaattca ccatatcttc atcacgcgta ccggggtgcg
    22921 cagagatgaa atacgggatc agatactgct ctttacctgc ctgtttcgag taggtatcga
    22981 acagctcttt aaagcggtca tagctgccca tgcccggctt catcatcttc gacaacggcc
    23041 cttcttcggt atgttccgga gcaatcttca gataaccgcc gacgtgatgg gtcgccaact
    23101 ctttgatata gcgcggatct tccacggcga tgtcataacg tacgcctgag gcgataagga
    23161 tttttttaat gcctttcaga tcacgcgcac ggcgatagag gttgatcgtc ggttcgtggt
    23221 tagtgtccat gtgcggacaa atatccggat aaacgcacga caaacggcga caagtttgtt
    23281 cagcgcgtgg cgatttgcag cgcaacatat acatgttggc agttggccca ccgagatcgg
    23341 aaatcacgcc cgtaaaacct ggaacggtgt cgcggatcgc ttcgatctca ttaatgatcg
    23401 aatcttccga acggctctga ataatgcgcc cttcgtgctc ggtaatagaa cagaaagaac
    23461 agccgccaaa gcagccgcgc ataatgttga ccgaaaaacg gatcatttcg taagccggaa
    23521 tacgggcatt gccataggcc ggatgtggca cgcgcttgta cggcagcgca aaaacgctgt
    23581 ccatctcttc ggtagaaagc gggatagcag gcgggttgat ccacacatag cggtcaccgt
    23641 gtttttgcat caatgcgcgg gcgcagcctg ggttggtttc gtggtgcaga atgcgcgaag
    23701 catgggcgta cagcacttta tcgcccttca ctttctcgaa agaaggcagc aatacgtagg
    23761 ttttttccca cggtttctgg cgcggtggct gcacgattac agctttcgct tcctgctttt
    23821 tcggcgcaac aggtttgtta tccgcacacg gcaaatcttc gccatacgga tgcgggattg
    23881 ggtcgatttt ccctagggta tcaagacggg tggaatccac accgctccag ccaggcagcg
    23941 cctcttttac gataattgcg gtattacgca catcgcggat ttcactaatc ggctcgccca
    24001 tcgccagacg atgcgctact tccaccagcg gacgttcacc gttaccaaac atcagcatgt
    24061 cggctttcga atctaccaga acggaacggc gcacggtatc ggaccagtaa tcataatgcg
    24121 cggtacggcg caggctagcc tcaataccgc cgaggatcac cggcacatct ttccacgcct
    24181 ctttacaacg ctgggtataa accagcgtgg cgcgatccgg tcgcttaccc gcgacattat
    24241 ctggcgtgta ggcatcatca tgacgtaaac ggcgatcggc ggtatagcgg ttgatcatcg
    24301 aatccatgtt gccagcagta acaccaaaaa acagattcgg tttacccaga cgcataaagt
    24361 cgtctttgct gctccagtcc ggctgggcga tgatcccgac gcgaaagccc tgcgcttcca
    24421 gcatacgacc gcaaatcgcc atcccgaagc ttgggtgatc gacatacgcg tcgccagtaa
    24481 ccaaaatgat gtcgcagcta tcccagccaa gttgatccat ctcttcacga gacatcggca
    24541 aaaacggtgc cggtccaaaa caggcggccc agtactgcgg ccaggagaac aggtcgcgat
    24601 ccggttggat cagggagata gagctcattt tgcttccaga aatgataaaa aaataatcaa
    24661 aggccgggga ttataagccg gaacgaaaga gaaatcgaaa ggtattccat actcgccctc
    24721 ctcgggcgag tatgaagatt acggtaccgg attgaccaac agttgcccaa tcgagccacg
    24781 gtccgccatt tccagcgtct gactgttgaa gtagaacggg aagtgcgccc aggaaggctg
    24841 accgaaataa acaagcagct ccacttgtcc gtcaacccaa acggtatctt tccagcctct
    24901 gtcttccggg aacggcatcg cgccattcac gttacggatc tggaacatta cgccttcaat
    24961 atggaacgct tgcggctcgt ccgcgcgtac cgtccagcgt tcccacgttc cttgctgcgc
    25021 ggtgacatca atacggttga cgtcccacag ctgtccatta atacccgggt catcacccag
    25081 actgatatcg cgactgcgaa ttggcgaacc agccatgatt tcagttggca gcaagcgcat
    25141 cggaagactg tctgtgacca gcggcagaag gccggttggg cgcagcgtta gcaccaaggt
    25201 agaaaccaga atactggatg gctcaaagaa gccgcgaata cgatcaacaa tgctcgccgc
    25261 ttcaccacag gtgatcgaca cttcatcgcc gttgctcata tccaccagaa tctcgcggcg
    25321 ctcgcccggt gccagcgaaa gttgcttcac cgatacagga gcaggcagga atccctgatc
    25381 gccagaaatc acatgtaacg ggcgaccatc gctcatctgt agttgatagc gacgagagtt
    25441 cgacgcgttc agcagtcgca agcgcaccca gccacgcgag acttcaacgt acgggctttg
    25501 tacaccgtta accagcagcg tatcaccaac aaagccgccg cttcccggtt cgttgtattc
    25561 tggcgtacca aagttatcca gccgtttatc ctggataatg accggaaaat catccacacc
    25621 ataatggttg gggataggca gcgacttgct gacttcatct tccaccagcc acattccggc
    25681 aaggccgtta tagacctgct gagccgtgcg gttgggagta ttggcgtgat accacagagt
    25741 agctgcgttc tggcgaatag gcagtacggg tgcccagtca gcgtttggcg acatcatccg
    25801 tgccggaccg cccatcagcg ggcctggtac ctgtagcccg gccaccgtca ttgagacatt
    25861 ttctgtcagg cggttgctgt aaataagctt aacatcgtcg cccttccaga cgcggatagt
    25921 cggccccagg taacgaccat tgattcccca gaccgacgcg cgtgtccctg gcgtaaatga
    25981 ccagtgcgca cgttgtacag tcataaacag cggttgccca cggcgagatt caagtagcgg
    26041 cggaacgggt agcggttgct gttgcccggc tgcgctggcc ttcaggggaa cagcgcctgc
    26101 acaaagtgca atccccgatg cctgaatgaa ctgacgccga ctgagtgaca tatttgctcc
    26161 atgtaaaact ggctaaagta ataccgtgca ggaaagtgtt ccccgcaaaa atacagattg
    26221 ccgacttaaa cttttccggc ggcttcgcgt tctgcgactt ctttatcgag ctcggcgatt
    26281 ttttgttcca ttatcgaacg acaatgggca gccagctcac gaacctgatc tttgccatac
    26341 tgactgacgt caattggcgg cagcatttcg acaatcacca gaccgttgtg cagtcgatta
    26401 agattaatct tattcgaagt tgtagagacg cacacgggaa taatcgggac gcccgccgca
    26461 attgccgcgt gaaatgctcc agtcttgaac ggtagcaggc cgcgaccacg gctgcgggtt
    26521 ccttccggga acatccagat ggaaatacgg cgttttttga aatgattcac gacttccgca
    26581 atggtgccgt gagctttagt gcgattgttt ctgtcgatca ataagttgcc ggttaaccag
    26641 tacaactgcc cgaagaaggg gatccacagc aagctctttt tacctaccgt caccgtcggc
    26701 ggttgcacaa tgttcgatgc tgtcaccatg tcatagttgt tctggtggtt agcgatatag
    26761 atagcattgc cgtagctttc agcatctgca ggtttgcggc actcaacttt caggccgaac
    26821 agtggcgcaa gacggccaaa catatgccca aaggtggcca catgtttcgg gttacgcggg
    26881 ctgaaaaggc agtaaatgga gccgaataca cagactaaga tgctgtaaat cacggtaata
    26941 ataagacgaa agatatatag cataacaccc tctgaaggtc tgagagactg gcattgtacg
    27001 tcacctgaaa tcaggttagg gcattgttaa aagccgctct ctggaaaagc gtattgttaa
    27061 tcgcaacgca tgaataaaca acggtttcaa gggaattttt atcgtttccg ggggttgaat
    27121 acgctgccgg atgacgactt aacgtttcat ccggcgttcc ttgctagcgg gaggaaacag
    27181 cgccctcccc ggcatattac tcttcgctat caccgctgct ggcacggcga ggagagtcga
    27241 tctcaacacg atcgatacgc tgcaaaccgc gcatcaacgt accgcggcgt ccacgttcgc
    27301 cagtgacttt ctgtagctct tccggacgca gtttaatttt gcgtttccca acatgaatgg
    27361 tcagcgtgct ttgcggcggc agaacgtaca gttgcgccag accatcctcg ccacgcgcgg
    27421 cttctgccga tggaatgttg ataatcttgt tgcctttgcc cttcgacagc tgcggcagat
    27481 cgcttaccgg gaacatcaac atacggcctg cctgagtgat tgccagcagc atatcggaag
    27541 catcttcaat caccaccggc ggcataacat gggcattttc cggtaaggtg atcaaagcct
    27601 tacccgcacg gttacgcgct accagatcgt taaaggtgca gacgaaaccg taacccgcat
    27661 cggaagccat cagcagtttc tgatcgtcgc tttccatcag catatggtca acggtcgccc
    27721 caggcggcaa cgttaatttg ccggtgagtg gttcgccctg accacgcgcc gacggcagcg
    27781 taatcgggtc gatggcatag ctacgaccgg tggaatcaac aaacactacc ggttggttac
    27841 tcttaccttt caccgccgct ttgaagctat cacccgcttt ataattcagg cccggcgcgt
    27901 cgatatcatg gcctttagcg ctgcgtaccc agcccatctg cgacagcaca atggtgacag
    27961 gttcagacgg cagcatgtcg tgctcgctca tcgctttcgc ttcttcgcgt tcctgcaacg
    28021 gcgaacgacg atcgtcaccg taggcttgcg cgtctgcctg cagttctttc ttcagcaggt
    28081 tattcatttt acgctcggaa gccaaaatgc cctgcaactg gtcgcgctct ttttccagct
    28141 cactctgctc accgcgaatc ttcatctctt ccagtttggc aagatgacgc agtttcagtt
    28201 cgaggatcgc ttccgcctgg gtttccgtaa ggccaaaccg cgacatcagc gccggtttcg
    28261 gttcatcttc gttacgaatg atctcaatca cttcgtcgat gttgagaaac gccaccagca
    28321 aaccttccag gatatgcagg cgcttgagga ctttctccag acgatagttc agtcggcggc
    28381 gcacggtatc gcgacggaac accagccatt cggagaggat ttccagcagg tttttcaccg
    28441 ccggacgacc atccagaccg atcatattga ggttaatgcg atagctcttt tccagatcgg
    28501 tggtagcgaa gaggtggttc atcacctgat ccatatccac gcggttggaa cgcggcacaa
    28561 tcaccagacg ggtcgggttc tcgtggtcag attcatcgcg cagatcgtca accatcggca
    28621 gctttttgtt gcgcatttgc gcagcaattt gctccagtac gcgcgcacct gaaacctgat
    28681 gcggtaatgc gctgataacc accgcgccat cttctttctt ccacaccgcg cgcatacgca
    28741 ctgaaccacg tccgttctcg tagattttac gaatctcggc gcgcgaagtg ataatttccg
    28801 cttcagtcgg ataatccggc ccctgcacga tatccagcag ctgatcgagc gtagttttcg
    28861 gctggtcgat taatgcgatt gccgcctgag ctacttcacg caggttatgc ggtggaatat
    28921 cggtcgccat gccgacggca ataccggtgg tgccgttaag caaaatgttt ggcagacggg
    28981 caggtagcat tttcggctcc tgcaaagtgc cgtcgaagtt tggcacccag tcagccgtcc
    29041 cctgccccag ctcgctcaat agcagctcgg aatatttcga caaccgggat tcggtgtaac
    29101 gcattgccgc gaacgatttc ggatcgtccg gcgcgcccca gttcccctga ccatcaacca
    29161 gcggataacg gtaagagaac ggctgcgcca tcaggaccat cgcttcataa caggcgctat
    29221 cgccgtgcgg atggtattta cccagtacgt caccgacggt acgggccgat tttttaaatt
    29281 tggcgctggc attcaggccc agttcagaca tcgcatacac aatgcggcgc tgaacaggtt
    29341 tcagaccatc accaataaac ggcaacgcac ggtccatgat cacgtacatg gagtagttta
    29401 agtaggcgtt ttccgtaaat tcatgtagcg caaggcgctc tgccatatcg ctcattaatt
    29461 ctgattcctc aacttattcg ccagccttca aaggggcaat attgccggag atactacatt
    29521 atctgccgcg tcgagtcaca ggagatcagc gtgaatagcg ccgccggacg tgctccgacg
    29581 gcgataaagg catcaatgtg ccttgatata caactctttg ctgtagactt gatcggcata
    29641 gagggcagca caaggttgcg cccatgccac accactcggc atgggggtcc gctccgggcg
    29701 attcgagaag ttgaggttat gcgagtccag ggaagcctaa agcatcaaag tagtcgactg
    29761 taagtatacg gttcagccca gtttagctgt gtctccacca gtgccccgcg cctcctgcca
    29821 taagtgcggc ttgctcatgg atatccccga gacggcttac ggcgcaccgg ataataaatt
    29881 tcgcgtatac atggtattcc ttaccggaga aattataagc ccaacaacaa tacgcctgtg
    29941 acattgttcg ctcaaactta tcgcgagtga tttggctcac actccggtaa agattcccca
    30001 tcacgccaac ggcacataaa catccgtttc cagtcgctgc tctgtcaccc cttcggcaag
    30061 attggtgtag tgaaacagaa tcggtgcttt acgcattttc tcgccgcttg caggcagcca
    30121 gtggcgaata atgccccata tcgtgtgtga aatatcgtcc agctcgccaa cgtggcgggc
    30181 cacggcataa cgtccaccgg taagttcacc attgctcaca ccataacgat tatcgggaat
    30241 cggttcgcta acgctgccgc agatatcaaa gcgaaacgct tccggtgcgg tggtggcagg
    30301 ggcgtcccag gcgacgccaa aagtctggct ttggttaaca ggagaaagcc ccgtctcttt
    30361 acgccacatg ataaatttcg ccacactgta gttgagcaac tcagggctac agcggtgcgg
    30421 caacatcgcc acagggattg atgggaaatc gataatgttt acatccagtg tcaggtttgt
    30481 cattttgcct ccctgcgaaa ttattcccgg gaacgcgtcc cgggagcggt aacaattacg
    30541 gatttacttt gcggatctgt ttgacgtcaa tttcaacaga attccagtct ttatcgactt
    30601 caccctgaat ctcaaccgta tctttcggcg tcaccgtcac accgttccag cgtttgtgat
    30661 cgatatcaac attgatagta ccgctggcat ctttaaacac gtagagatcg tcagagatgc
    30721 gttcaacgat attgccgcgc agggttaccc aggtgtcgtc acgcagggat tttgcgcttt
    30781 ctacagtcgt tacgctgccg ttcggcccct gaaatcctcc ggcctgactt tgcgttaccg
    30841 atgggccaga aaaaccgccc tgctctgctg ccatcaccgg tgcactgcac agggccatta
    30901 ctgcgattac tgctgcgaat tttttcatgt ttattactcc ctttaatgtc tgtttccgag
    30961 catttaacaa gatagtcctt aacaacttct taagggaaaa aaataaaatt taatgctgta
    31021 cagagcgcgt tacaacacgg tttactggca gcaaatacgg ttatcgcagg gatgaaaaaa
    31081 tgcgaatttt actgatagaa gatgacatgc tgattggtga cggcatcaaa actggcctta
    31141 gtaaaatggg ttttagtgtc gactggttta cacaaggtcg tcagggaaaa gaggcgcttt
    31201 atagcgcacc ttatgatgcg gtgatcctgg atttaacctt accaggcatg gatggtcgcg
    31261 atattttgcg cgaatggcga gagaaaggtc agcgtgagcc ggtactgaac ctgaccgcgc
    31321 gtgatgcgtt agcggaacgt gtagaagggc tgcgtctggg agctgacgat tatctgtgta
    31381 aaccttttgc gttgatagaa gtcgccgcca ggctggaggc tctgatgcgc cgaaccaacg
    31441 gccaggccag caacgagctg cgccacggta acgtcatgct cgaccccggc aaacgtatcg
    31501 ccacgctggc tggcgaaccc ttaacactga aaccaaaaga atttgccctg ctggaattac
    31561 tgatgcgtaa cgctggtcgg gtactgccgc gcaaactgat tgaagagaaa ctgtatacct
    31621 gggacgaaga ggtcaccagt aatgccgttg aagtgcatgt gcatcatctg cgacgcaaac
    31681 tcggtagtga ttttattcgt accgtgcatg gtattggcta cacattaggt gagaaatgaa
    31741 atttacccaa cgtcttagtc tgcgcgtcag gctgacgcta atctttttaa ttctggcctc
    31801 ggtgacctgg ctgctttcca gctttgtcgc ctggaaacaa acaacggata acgtcgatga
    31861 attgttcgac acccaactga tgctgtttgc caaacggtta agtacgctcg atctcaacga
    31921 aatcaacgcg gcggatcgca tggcacagac gccaaataaa ttaaaacacg gtcatgttga
    31981 tgacgatgcg ctgacctttg ccatctttac ccacgacggc agaatggtcc ttaatgatgg
    32041 cgataacgga gaagatattc cctatagcta tcaacgggaa ggttttgctg acgggcaact
    32101 ggtcggtgaa gacgatcctt ggcgttttgt ctggatgacc tcacctgatg gcaaatatcg
    32161 catcgttgtt ggccaggaat gggaataccg tgaagacatg gcgctggcga ttgttgccgg
    32221 gcaattgatc ccgtggctgg tcgcactgcc gattatgtta atcatcatga tggtactact
    32281 gggtcgtgaa ctcgcgccgc tgaacaaact ggcgctggca ctacgtatgc gtgaccctga
    32341 ctcggaaaaa ccactaaacg cgactggcgt acccagcgaa gtgcggccac tggttgagtc
    32401 gctaaatcaa ctgttcgccc gcacacatgc gatgatggtt cgagaacgac gctttacctc
    32461 cgatgcagct cacgaactac gtagcccgtt aacagcgctg aaagtgcaaa ccgaagttgc
    32521 gcagctctct gacgatgatc cgcaggcgcg gaaaaaagca ctgctccaat tacattccgg
    32581 gatcgatcgc gctactcgtc tggttgatca actgctcacg ctatcgcggc tggactcact
    32641 ggataacctt caggacgtcg cggagatccc gcttgaagat ctcctacaat cgtcggtgat
    32701 ggatatttac cacacggcgc agcaggcgaa aattgacgtg cgactgacac tcaatgccca
    32761 cggcatcaaa cgcaccggac aaccgctatt gctaagtttg ttggtgcgaa atttgctgga
    32821 taacggcccc ggtgtgacac cagaggcact ggcgcgaatt ggcgaacgct tctatcgccc
    32881 acccggacaa accgctaccg gcagtggact tgggctatcg attgtccagc gaatcgccaa
    32941 gctgcatgac atgaatgttg agtttgggaa tgcggaacaa ggtggatttg aggcgaaggt
    33001 aagctggtaa cttctctgtc tatgagagcc gttaaaacga ctctcataga ttttactaat
    33061 agcaaaatat aaaccgtccc caaaaaagcc accaaccaca aaccagacaa ataacgagcg
    33121 agaatacata tagccaaaac tcgaacattt cccgcataaa gagttttctt aagataagaa
    33181 taataagtgg cgtaagaaga aaaaatgctg caatcattgc ttgcaatata cgatcttcgc
    33241 ctgaaacgga aggtatactg caaatattgc ctcccgactc aagcatccag tcatacttat
    33301 tgaaagcaaa aaaagaatct attaaccaat aagtaagcat tatatatggc aatgcttcaa
    33361 atattgtttt aattttctgc tttaacatat atcagtacgc tcataaaaat ccatttataa
    33421 ttatcaacat gacacgttga tattttcatc ttttacttat tcaaagccat attttcaatc
    33481 cccaaaaaaa ccgaaccaaa cgtgtaaatt attccggtgc gagtaagaat tatgaggaat
    33541 ggctatcagt attgtcattt tcagaaaata tttatcctgc atcggtgagt cagagctaga
    33601 tcagactttt gctaaattcg caaaagactt tgcacatttt gctaatttca cagtaccgct
    33661 ctgtgacgta ctatagtcga caaacgtctc accttgagat taaaaaatga gcaacatcct
    33721 gattatcaac ggcgcgaaaa aattcgccca ctccaatggt caactgaacg acaccctgac
    33781 cgaagtcgcg gatggcacac tgcgcgacct cgggcatgat gtccgcatcg ttcgcgccga
    33841 cagcgactac aatgtcaaag cggaagtaca aaactttctc tgggctgatg tggtgatctg
    33901 gcagatgcca ggctggtgga tgggcgcgcc gtggacagtg aaaaaatata ttgatgatgt
    33961 attcaccgaa ggtcacggga ctctgtatgc cagcgatggt cgtacccgca aagatccgtc
    34021 gaaaaaatac ggttccggca gcctggtaca ggacaaaaaa tatatgcttt ctctaacctg
    34081 gaacgcacca atggaagcct tcaccgaaaa agatcagttc ttccacggcg ttggcgttga
    34141 cggtgtgtat ctgccgttcc ataaagcaaa ccaattcctc ggtatggaac cgctgccgac
    34201 atttatcgct aatgacgtga taaaaatgcc tgatgttccc cgctatactg aagaatatcg
    34261 caagcatctt gtggaaattt ttggttaact agagctcagg ctttagaagg agttaaccat
    34321 gcttaccgta atcgcagaaa tccgtactcg tcctggtcaa catcaccgtc aggcggtatt
    34381 ggatcagttt gctaaaatcg ttccaaccgt actgaaagaa gaaggttgcc acggctatgc
    34441 gccaatggtg gattgcgctg ctggcgtgag tttccagtct atggcaccgg attctatcgt
    34501 gatgattgag cagtgggaaa gcatcgcgca ccttgaagcg catctgcaaa ccccgcacat
    34561 gaaggcgtac agcgaagccg taaaaggcga cgtgctggag atgaatatcc gtattctgca
    34621 gccagggatt taatcctgcc ttgtttgccc ggccatcctg accgggcaat gttctttcct
    34681 ttaaacctca atctccgcca tgtcgccttt ctcttgcaac cagttgcggc gatcttccga
    34741 gcgtttcttc gccagcagca tatccatcat cgcgtcagta cgctgatcgt cttcatcatc
    34801 gatagtcaac tgcaccagac ggcgagtgtt cggatcaagc gtggtttcac gcaattgcat
    34861 cgggttcatt tcccccagac ctttaaaacg ctggacgttc ggcttgcctt tcttgcgttt
    34921 taattgctca agtacgccct ctttctcttc ttccgtcagc gcgtaataaa cctctttccc
    34981 gagatcaata cggtagagcg gtggcagtgc gacgtaaacg tgaccgtgtt tcaccaacgc
    35041 gcggaagtgt tttacgaaca aagcgcagag cagcgtggca atgtgcagac catcagagtc
    35101 cgcatccgcg aggatacaga ttttgccata acgaagctgg ctcagatcgt cgctgtcagg
    35161 atcgataccg atcgctaccg aaatatcatg cacttcctgc gaggccagca cttcgtcgga
    35221 agaaacttcc caggtgttaa ggatcttacc tttcagcggc atgatcgcct gatattcacg
    35281 atcgcgcgcc tgcttggcag atccgcctgc ggagtcacct tccacgagga acagttcggt
    35341 acggttaagg ccctgcgcgg tacaatcagc cagtttgcca ggcagcgccg ggccgctggt
    35401 cagcttttta cgcaccactt ttttagccgc acgcatacga cgctgggcgc tggaaatcgc
    35461 catctctgcc agcaactccg ccgcctgaac gttctggttc agccacagga tgaaggcatc
    35521 tttcaccacg cccgaaacga atgccgcaca ctgacgcgaa gagagacgct ctttggtctg
    35581 cccggcaaac tgcggatcct gcattttaac cgacagaaca taggcgcagc gatcccagat
    35641 atcttccgcc gacagcttca cgccgcgcgg cagaatgttg cggtattcac agaactcacg
    35701 catcgcgtcc aacaggccct gacgcagacc attaacatgg gtaccgccct gcatcgttgg
    35761 gataaggttg acgtagcttt cggtcagcag ttcaccgcct tccggcagcc acagtagcgc
    35821 ccagtccaca gcttcagtat cgccagcgaa attaccgata aacggttttt ctggcagcgt
    35881 cggtaaaccg tttaccgctt ccgccaggta atcattcaga ccgtcctgat agcaccagcg
    35941 ctgttcggtg ttgttgatct catctttaaa ggtaatctca acgccggggc acagtactgc
    36001 tttggccttc agcacatgcg tcaggcgtga aacagaaaaa cgcgggctgt caaagaaggt
    36061 ttcatccggc cagaagtgca cgctggtacc ggtattgcgt ttaccgcaag tgccgacaac
    36121 ctgtaaatcc tgcacctttt cgccattttc aaaggcgatg ttataaacct gaccatcgcg
    36181 gcgcacgtta acttctacgc gcttcgacaa ggcgttaact accgaaatcc ccacgccatg
    36241 caggccgcca gagaactggt aatttttgtt agagaattta ccgcctgcat gcagacggca
    36301 aagaatcagt tcaaccgccg gtactccttc ttccgggtga atatccaccg gcatcccgcg
    36361 cccatcgtca atcacttcta acgactggtc agcatgtaag attacatcca cgcgttttgc
    36421 gtgacccgcc agtgcttcat cgacactgtt atcaataact tcttgcccca aatggttagg
    36481 gcgagtggta tcggtataca tccccggacg gcggcgaacc ggctcaagcc cggtgagtac
    36541 ctcaatggca tcagcgttat aagtttgcgt catggtttaa gttagcaatt cgagttgatc
    36601 gtcagagatg gtgcagacca agaaaatcga cgatcgggtt gaaataatct tcgaagcccg
    36661 tgaatgcgtg gttgccgcct tctatgacag tctggcggca ggaagcatag tacgccaccg
    36721 cctggcggta atccagcact tcatctcccg tctgttgcag cagccagatc aaatccggcg
    36781 cttccagcgg gtcaatctgc atgactttga gatcgtaaat atggcgtgac tctagcacat
    36841 attgctgccc ggtgtagggg ttctcgttct gaccgagata gtccgtcagc aattcaaacg
    36901 ggcgtaccgc cgggtttacc accactgcgg gcagcataaa acattgcgac aaccaggtgg
    36961 cgtaatatcc ccccagtgac gaaccgacaa tacccagcga atcgccgcca tgttccagga
    37021 caatggattc cagcagttct gccgcgtcgg aaggatacgg cggcaactgc ggaatgatca
    37081 tctcaacgtc agggtgatgt tccgccagcc agttttttaa caagctcgct tttgcagagc
    37141 gcggcgagct gttgaaaccg tgtaaataaa gaagcgtaga catcagtagc cttctgaagc
    37201 ggtatcaggt tggaaacgtg tgtccgccag gcgatgcacc tcggtggtca gcgtgccatc
    37261 agcatgtaac tcgagagtac gccagccggg cgcgatagta tccagcgtaa agttggaaca
    37321 gtgcggctta aactgcacac aggtcgacgg cgttgccagc aggcggcgac cgttccagtc
    37381 gagatccagc tcctgatgaa tatgaccgca cagcaagtat ttgacgtgcg gaaacttcgc
    37441 cagcacggta tccagttcgc ccgcgttacg cagactgtgt tgatcgagcc aactacaacc
    37501 cgcaggtagc ggatgatgat gcagcagcag caacgtatgg cgttctggcg catcggccag
    37561 tttacgttcc agccactcaa gctgaaactc gctcagctca ccgtgcggca cgccaaacac
    37621 ctggctatcc agcaacagga tttgccattg ctcaccaata aacacccgct tcgccgggga
    37681 gatacccgca tcctgtaacg cgctgtacat cgcgggctgg aaatcgtggt tgcccggcag
    37741 ccagacgcag ggcgcacgaa aacttgcgat gccttcagcg aaatgctgat aggccgcaga
    37801 ggattgatcc tgcgctaaat cacctgtcgc gacaatcagg tcgaattcgt gctggtgtgg
    37861 acgaatcgcc tccagcaccg cctggtagct ttcccaggtg tttaccccta acagggcttc
    37921 gtgcttttgt gcaaacaggt gagtatcggt aatttgtaaa atccttcctc tggcctcacc
    37981 agccagagga agggttaaca ggctttccaa atggtgtcct taggtttcac gacgctagta
    38041 aaccggaatc gccatcgctc catgtgctaa acagtatcgc aaccagtccg ctaaaaactg
    38101 gttaatttga tgcttttcgt cgcgttgatg caacttttta ttaggataat cataccgcgc
    38161 tttgaagcga aaaatctgct ggcttgaaca cacttcagcc accatcgcgt catgatacag
    38221 acgcaccgtc attgacggaa ggctccagta actgatcgcg ggcgcagtct gttctattgt
    38281 caccagggta gtgtatcggg tcgattccac aatcgtcagc cgatattgtg cgtttgccac
    38341 ctgatagctt acagtttcgc cgggtgcgtc attgcgcggt aacaaacggc gcaattgtga
    38401 aaagttcatc tcgcacaggc gcatcatttc aggaaagtca ggtgtgtaac gcttcattta
    38461 tgcccactca ttttttaacg cttgatgatg cagctgcagc cattgcaaag cgatgaccga
    38521 cgctgcgttg tcgattttcc cctcttctac ccactggtat gcctgttccc ggcttaccac
    38581 atgaacgcga atatcttcgt tttcatcagc cagaccatga ataccgcttg cggtcgtggc
    38641 gtccacttcg cccaccataa ttgacgaacg ctcactggtg ccccccgggc ttgccaggaa
    38701 acttaacacc ggtttggtcc gtttgactat cagtcccgcc tcttcaatcg cttcgcgacg
    38761 ggcaacatct tccacacttt caccctcttc aatcatcccg gcaaccatct ccagtagcca
    38821 gggggtttcg ctggtgtcgt acgcggcaat ccgaatctgc tcaatcagca caacttcatc
    38881 acgcactggg tcaaagggta gcaagactgc ggcgtgaccg cgctcaaaaa tttcccgccg
    38941 tacctcatga ctcatttgcc cgttgaatag acgatgacga aatctataaa gatctaatga
    39001 aaaaaagccg cgataaagtg tttctcgtgc aataatttct acatcgtttt tgccaaatgt
    39061 aacgggcagg ttgtctggct taagcattgt taatgtccta gcactaatag tgaattaaat
    39121 gtgaatttca gcgacgtttg actgccgttt gagcagtcat atgttaaatt gaggcacatt
    39181 aacgccctat ggcacgtaac gccaaccttt tgcggtagcg gcttctgcta gaatccgcaa
    39241 taattttaca gtttgatcgc gctaaatact gcttcaccac aaggaatgca aatgaagaaa
    39301 ttgctcccca ttcttatcgg cctgagcctt tctgggttca gttcgttgag ccaggccgag
    39361 aacctgatgc aagtttatca gcaagcacgc cttagtaacc cggaattgcg taagtctgcc
    39421 gccgatcgtg atgctgcctt tgaaaaaatt aatgaagcgc gcagtccatt actgccacag
    39481 ctaggtttag gtgcagatta cacctatagc aacggctacc gcgacgcgaa cggcatcaac
    39541 tcgaacgcga ccagtgcgtc cctgcagtta actcaatcca tttttgatat gtcgaaatgg
    39601 cgtgcgttaa cgctgcagga aaaagcagca gggattcagg acgtcacata tcagaccgat
    39661 cagcaaacct tgatcctcaa caccgcgacc gcttatttca acgtgttgaa tgctattgac
    39721 gttctttcct atacacaggc acaaaaagaa gcgatctacc gtcaattaga tcaaaccacc
    39781 caacgtttta acgtgggcct ggtagcgatc accgacgtgc agaacgcccg cgcgcagtac
    39841 gataccgtgc tggcgaacga agtgaccgca cgtaataacc ttgataacgc ggtagagcag
    39901 ctgcgccaga tcaccggtaa ctactatccg gaactggcgg cgctgaatgt cgaaaacttt
    39961 aaaaccgaca aaccacagcc ggttaacgcg ctgctgaaag aagccgaaaa acgcaacctg
    40021 tcgctgttac aggcacgctt gagccaggac ctggagcgcg agcaaattcg ccaggcgcag
    40081 gatggtcact taccgactct ggatttaacg gcttctaccg ggatttctga cacctcttat
    40141 agcggttcga aaacccgtgg tgccgctggt acccagtatg acgatagcaa tatgggccag
    40201 aacaaagttg gcctgagctt ctcgctgccg atttatcagg gcggaatggt taactcgcag
    40261 gtgaaacagg cacagtacaa ctttgttggt gccagtgagc aactggaaag cgcacatcgt
    40321 agcgtcgtgc aaaccgtacg ttcctccttc aacaacatta atgcttctat cagtagtatt
    40381 aacgcctaca aacaagccgt agtttccgcg caaagctcat tagacgcgat ggaagcgggc
    40441 tactcggtcg gtacgcgtac cattgttgat gtgttggatg cgaccaccac gctgtacaac
    40501 gccaagcaag agctggcgaa tgcgcgttat aactacctga ttaatcagct gaatattaag
    40561 tcagccctgg gtacgttgaa cgagcaggat ttgctggcac tgaacaatgc gctgagcaaa
    40621 ccggtttcca ctaatccgga aaacgttgcc ccgcaaacgc cggaacagaa tgctattgct
    40681 gatggttatg cgcctgatag cccggcaccc gtcgttcagc aaacatccgc acgcactacc
    40741 accagtaacg gtcataaccc tttccgtaac tgatgacgac gacggggctt cggccccgtc
    40801 tgaacgtaag gcaacgtaaa gatacgggtt atctgccgca ttcttccccc ttctcgcttc
    40861 aatttcgacc agccatcctc tattctgatg ggtatttacc actggtcccg gaagacaaaa
    40921 atgaaacgga caaaatccat acgccacgca tcgttccgca aaaactggag cgcacgccat
    40981 ctgacaccag tcgctctcgc ggttgccact gtttttatgc tggctggctg tgaaaagagt
    41041 gatgaaacag tgtctctcta tcaaaatgct gacgactgtt cagctgcaaa cccaggcaaa
    41101 agcgccgaat gtaccaccgc gtacaacaat gcgctgaaag aagccgaacg tactgcgccg
    41161 aaatacgcca cccgtgaaga ctgtgttgct gaatttggtg aaggtcagtg tcagcaagca
    41221 ccagcccagg ctggcatggc accagaaaac caggcgcagg ctcagcaatc cagcgggagt
    41281 ttctggatgc cgctgatggc cggttacatg atggggcgtc tgatgggcgg cggcgcggga
    41341 tttgcacagc agccgctgtt ctcctcgaaa aacccggcca gtccggctta cggtaaatat
    41401 accgacgcga cgggtaaaaa ctatggcgca gcccagccag gccgcaccat gaccgtaccg
    41461 aagacggcaa tggcaccaaa accggcgacc accactaccg ttacccgtgg cggttttggt
    41521 gaatctgttg ccaaacaaag cactatgcag cgtagcgcaa ccggcacctc ttctcgttca
    41581 atgggtggct gataccgatg gaaagagtca gtattaccga gcgcccggac tggcgtgaga
    41641 aagcccacga atacggtttc aattttcaca ccatgtacgg cgagccgtac tggtgtgaag
    41701 atgcttacta caagttgacc ctcgcccagg ttgaaaagct ggaagaagtc accgccgaac
    41761 tgcaccagat gtgcctgaaa gtggtggaaa aagtgatcgc cagcgatgag ctgatgacca
    41821 aattccgcat accaaaacac acctggagtt ttgtgcgtca atcatggctg actcaccagc
    41881 catcgcttta ttcgcgtctt gatctggcgt gggatggcac tggcgaacct aaacttctgg
    41941 aaaataacgc cgatacgcca acgtcactat acgaggcggc gttctttcag tggatctggc
    42001 tggaagatca gcttaacgcc ggtaacttgc cggagggcag cgaccagttt aacagtctgc
    42061 aagaaaaact gatcgatcgc ttcgttgagc tgcgtgaaca gtatggcttc cagttgctgc
    42121 atctcacctg ctgtcgcgac acggtggaag atcgcggaac cattcagtat ttgcaggact
    42181 gcgcaaccga agctgaaatc gccaccgagt tcctctacat cgacgagata ggtttaggcg
    42241 aaaaaggcca gttcacggat ttacaggatc aggtgatttc taacctgttc aagctttatc
    42301 cgtgggaatt tatgctgcgc gaaatgttct ccaccaagct ggaagatgct ggcgtacgct
    42361 ggctggaacc ggcgtggaag agcattatct ccaacaaggc acttctaccg ctactgtggg
    42421 agatgttccc gaatcacccg aacctgctgc ccgcttattt tgcggaagat gatcatccgc
    42481 aaatggaaaa atatgtggtt aaaccgatct tctcccgtga aggcgcaaac gtgtcgatca
    42541 ttgagaacgg caaaaccatt gaagcagcgg aaggtccgta tggcgaagaa gggatgattg
    42601 ttcagcaatt ccacccgtta ccgaaattcg gcgacagcta tatgctgatt ggtagctggc
    42661 tggtgaacga tcaacctgcc ggaattggca ttcgtgaaga ccgtgcgttg atcacccagg
    42721 atatgtctcg gttttatcca catatttttg ttgaataagc cacgataccg gatggcactc
    42781 gccatccggt aattgttagc ctatctgcac cgacagcata cttaaactgc ccatttctat
    42841 accatcgacc ggaatggtaa ttggctcctg cccatcccac acacctaaca catacaacaa
    42901 cggcagatag tgctctggcg ttgggttcga taacgcgcca ccttcatggt cgagataatt
    42961 caccagagga tgctgctcta ccggcccttg ccacgtcaga ttcgctttca catactcatt
    43021 aaacgacgtc gcccacggat acggtgaact atcaccgtgc cacttcactg tgcgcaggtt
    43081 atgcaccacg ttaccgctgg cgaccaacat tattccttca tcacgcagcg ccgcgagttt
    43141 gcgccccatt tcgaaatgcc aggcggcagg tttgctactg tcgatactca actgcaccat
    43201 agggatatcg gcgtcaggat acatcttaat cagcacgccc cacgagccgt ggtcaaagcc
    43261 ccaggcttct ttatccagcg tcaccgggat cggcgctaac agctcaacca gacgctgtgc
    43321 cagcgcaggc gaacccggag caggataatg cgtatcgtac agcgcctgcg ggaagccacc
    43381 aaagtcatga atcgtgggcg gcgtctccat cgcggtcact cctgttccac gggtaaacca
    43441 gtgagccgaa accaccacaa tcgcttgcgg gcgtggcaat gtcatcccca acttctgcca
    43501 gctgcgggta tacaaattat cttccagcac gttcatcgga ctaccgtgac ctaaaaacaa
    43561 tgctggcata cgtgttgaag acatgatgat atccttaact aaaggtgtca ttttgatatc
    43621 ctcacaatac gcttgttcgg cggagtaaga acccggataa caatgatgat gatcatcagt
    43681 tattttgacg atctgcctga aggtgaagat ttataaggag ttgtcgatgt cagtacctct
    43741 cattctgacc atactggcgg gggcagccac gtttattggc gcgtttctcg gcgttctcgg
    43801 acaaaaaccc tcgaaccgct tactggcgtt ttcgctaggt tttgcggcgg ggatcatgtt
    43861 gctcatctca ttaatggaaa tgcttcctgc cgcactagca gctgaaggaa tgtcgccggt
    43921 gttgggttat ggaatgttta tcttcggtct gcttggctac tttggcctgg accgcatgtt
    43981 gccgcatgcg catccgcagg atttaatgca aaaatcggtg cagctgttgc caaaatcgat
    44041 caagcgcaca gccattctgc tcactctcgg catcagtctg cataacttcc cggaagggat
    44101 tgccaccttt gtcacggcga gcagcaacct ggagctggga tttggcatcg cactggccgt
    44161 cgcgttgcac aatatccctg aaggtctggc agtggcaggc ccggtttatg cggcaacggg
    44221 gtctaaacgc accgcgattc tgtgggcggg gatttctgga ctagcagaaa ttcttggtgg
    44281 tgtgctggcg tggttaatcc tcggtagcat gatttccccg gtggtcatgg cggcaatcat
    44341 ggcggcggtt gcaggaatta tggtggcgct ctcggttgat gaattaatgc cgctcgccaa
    44401 agagattgac cctaataata accccagcta tggcgtacta tgtggaatgt cagtgatggg
    44461 attcagttta gtgctgctac aaacggcggg aattggttaa aaacaacatc agaagttgtc
    44521 cagcacatgg acaacttctg ccagcttcac gaaagcctta ttctaccttt tcggctgtct
    44581 cttctctcgt actgtttaag tatttgttcc gcgcttcaag cagtgcgtct ctgtcctggc
    44641 gaaacgcccg acaataacgt ttcaaatgca ggaaataacc caccacaacc agagcaatca
    44701 atacaatcca gtaccaggcg ataaacatcc ttttaccctt aatatttaac aatactaaac
    44761 atttagcgca taaatttcac atatcctttt tcggatatat tcatatggtc gcataataac
    44821 ggaacaatta tatgattttg atcataatga aaaagaatgc ctgatattaa tgaaattttc
    44881 tcatagaaag agaagcggaa ttaacatctt gcatagcagg gcaaaataaa aaccgaagcc
    44941 agctacaact tcggtttctt gattaaggca gtaattaagc agcggttttc agctggcttt
    45001 acgctcatgt gcctgacggt atgccaccag gtcttcaata gtcacgagcg ccatattgtg
    45061 tttattggca aactcaatgc actctggtgc acgcgccatc gtgccatcgt cattagtcag
    45121 ctcacacagc acaccagccg gtttaaagcc tgccagcgtc atcaaatcaa tagtcgcttc
    45181 agtatgaccg ccacgcgtca gtacaccacc tgcctgcgcg cgaagtggga aaacgtggcc
    45241 cgggcgattc agatcagaag gttttgcgcc atcggcaatc gctgcgcgaa cggtcgtaat
    45301 acgatcagcg gcagaaacac cggtagtcac accttcagcc gcttcaatgg tcacggtaaa
    45361 accggtgcca taggcgctgg tgttattttc taccatcatt ggcagatcaa gttgtttacg
    45421 gcgatcttca gtgatgcaca ggcaaacaat accgctaccg tggcgaatgg tcagcgccat
    45481 ctgctcaacg gtcatggttt ctgccgggaa gatcatatcg ccttcgtttt cacggtcttc
    45541 atcatcaagc accattacac cgcgtccttc acgcagcgca gccagtgcat tttcaacacg
    45601 ttcgaaaggc gtaccaaaag aggaaagtag cgtctgattc atggtaaaaa aacctcacta
    45661 aaattatggt taccagaatc agggcagtct taggagtggc ggcatatagc caaaataacg
    45721 tgagcgggtc catgcccgac agaatcgtta ctctctccca tccggactct aaccgtcggc
    45781 cccggaatta caccggatct gctgtccttt gagttcgcac ccaaagcgct cgcgggcttt
    45841 caactgagtt gatttaccgc cggtggggaa tttcgccccg ccctgagaat aagcggattc
    45901 actataacgc taatgattag cggcagcaac gcatagcttc acataattct ggtttatgac
    45961 ttacccttat cgcactacaa tggtactcaa cacctatcag tacagggaaa ctacaatgat
    46021 tgacccgaaa aaaattgagc aaatcgctcg ccaggttcac gaatcaatgc ctaaaggaat
    46081 cagggagttc ggggaagatg tggagaaaaa aatccgccaa accctacaag ctcagctgac
    46141 gcgcctcgac cttgtaagcc gtgaagagtt cgacgtccaa acgcaagtcc tgttacggac
    46201 tcgtgagaaa ctggcgctgc ttgaacaaag aatcagcgaa ctggaaaatc gcagtactga
    46261 gatcaaaaag cagccagatc cagagactct ccccccaacg ctgtaaacat ttgt
//
LOCUS       DAZSIO010000013        44233 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZSIO010000013 DAZSIO010000000
VERSION     DAZSIO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.013"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..1734
                     /gene="adeD"
                     /locus_tag="ACHX5T_000627"
     CDS             <1..1734
                     /gene="adeD"
                     /locus_tag="ACHX5T_000627"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR8465953.1"
                     /translation="ISRAEYQELLAVSRGDAVADYIIDNVSILDLINAGEISGPIVIK
                     GRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLT
                     TVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQM
                     LAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGLGGKELNAYIAA
                     GIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNSPQCMLCTDDRN
                     PWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVL
                     LSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQPVSASDFALQFT
                     PGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRLAPACGLLGGFG
                     LNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIA
                     GLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGE
                     KFAFTTLEVTE"
     gene            complement(1780..3171)
                     /gene="uhpT"
                     /locus_tag="ACHX5T_000628"
     CDS             complement(1780..3171)
                     /gene="uhpT"
                     /locus_tag="ACHX5T_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR8465954.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3309..4628)
                     /gene="uhpC"
                     /locus_tag="ACHX5T_000629"
     CDS             complement(3309..4628)
                     /gene="uhpC"
                     /locus_tag="ACHX5T_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR8465955.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4638..6140)
                     /gene="uhpB"
                     /locus_tag="ACHX5T_000630"
     CDS             complement(4638..6140)
                     /gene="uhpB"
                     /locus_tag="ACHX5T_000630"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR8465956.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6140..6730)
                     /gene="uhpA"
                     /locus_tag="ACHX5T_000631"
     CDS             complement(6140..6730)
                     /gene="uhpA"
                     /locus_tag="ACHX5T_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR8465957.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6805..7095)
                     /gene="ilvN"
                     /locus_tag="ACHX5T_000632"
     CDS             complement(6805..7095)
                     /gene="ilvN"
                     /locus_tag="ACHX5T_000632"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR8465958.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7099..8787)
                     /gene="ilvB"
                     /locus_tag="ACHX5T_000633"
     CDS             complement(7099..8787)
                     /gene="ilvB"
                     /locus_tag="ACHX5T_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR8465959.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8893..8991)
                     /gene="ivbL"
                     /locus_tag="ACHX5T_000634"
     CDS             complement(8893..8991)
                     /gene="ivbL"
                     /locus_tag="ACHX5T_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR8465960.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9556..9645
                     /gene="tisB"
                     /locus_tag="ACHX5T_000635"
     CDS             9556..9645
                     /gene="tisB"
                     /locus_tag="ACHX5T_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR8465961.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9769..9843)
                     /gene="ysdE"
                     /locus_tag="ACHX5T_000636"
     CDS             complement(9769..9843)
                     /gene="ysdE"
                     /locus_tag="ACHX5T_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR8465962.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            9925..11109
                     /gene="emrD"
                     /locus_tag="ACHX5T_000637"
     CDS             9925..11109
                     /gene="emrD"
                     /locus_tag="ACHX5T_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR8465963.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11117..11614)
                     /locus_tag="ACHX5T_000638"
     CDS             complement(11117..11614)
                     /locus_tag="ACHX5T_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR8465964.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11611..11973)
                     /locus_tag="ACHX5T_000639"
     CDS             complement(11611..11973)
                     /locus_tag="ACHX5T_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR8465965.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(11963..12310)
                     /locus_tag="ACHX5T_000640"
     CDS             complement(11963..12310)
                     /locus_tag="ACHX5T_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR8465966.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12419..12832
                     /gene="yidI"
                     /locus_tag="ACHX5T_000641"
     CDS             12419..12832
                     /gene="yidI"
                     /locus_tag="ACHX5T_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR8465967.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(12879..14321)
                     /locus_tag="ACHX5T_000642"
                     /pseudo
     CDS             complement(12879..14321)
                     /locus_tag="ACHX5T_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(14356..16071)
                     /locus_tag="ACHX5T_000643"
     CDS             complement(14356..16071)
                     /locus_tag="ACHX5T_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFR8465968.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            16238..17131
                     /locus_tag="ACHX5T_000644"
     CDS             16238..17131
                     /locus_tag="ACHX5T_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR8465969.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(17128..17942)
                     /locus_tag="ACHX5T_000645"
                     /pseudo
     CDS             complement(17128..17942)
                     /locus_tag="ACHX5T_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17942..19558)
                     /locus_tag="ACHX5T_000646"
     CDS             complement(17942..19558)
                     /locus_tag="ACHX5T_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR8465970.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            19853..20527
                     /locus_tag="ACHX5T_000647"
     CDS             19853..20527
                     /locus_tag="ACHX5T_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR8465971.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(20566..22227)
                     /locus_tag="ACHX5T_000648"
     CDS             complement(20566..22227)
                     /locus_tag="ACHX5T_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR8465972.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(22423..22851)
                     /gene="ibpB"
                     /locus_tag="ACHX5T_000649"
     CDS             complement(22423..22851)
                     /gene="ibpB"
                     /locus_tag="ACHX5T_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR8465973.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(22963..23376)
                     /gene="ibpA"
                     /locus_tag="ACHX5T_000650"
     CDS             complement(22963..23376)
                     /gene="ibpA"
                     /locus_tag="ACHX5T_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR8465974.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(23370..23442)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            23681..24013
                     /locus_tag="ACHX5T_000651"
     CDS             23681..24013
                     /locus_tag="ACHX5T_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR8465975.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(24015..25247)
                     /locus_tag="ACHX5T_000652"
     CDS             complement(24015..25247)
                     /locus_tag="ACHX5T_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR8465976.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTWTPDSQWLVFDVRPSGASFT
                     GETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHR
                     RGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPA
                     LDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVG
                     NHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQR
                     RLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTH
                     NKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDG
                     QWLAWMEGGQLWITETDR"
     gene            25348..26448
                     /gene="cbrA"
                     /locus_tag="ACHX5T_000653"
     CDS             25348..26448
                     /gene="cbrA"
                     /locus_tag="ACHX5T_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR8465977.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(26409..27746)
                     /locus_tag="ACHX5T_000654"
     CDS             complement(26409..27746)
                     /locus_tag="ACHX5T_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8465978.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(27821..28969)
                     /gene="dgoD"
                     /locus_tag="ACHX5T_000655"
                     /pseudo
     CDS             complement(27821..28969)
                     /gene="dgoD"
                     /locus_tag="ACHX5T_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(28966..29583)
                     /gene="dgoA"
                     /locus_tag="ACHX5T_000656"
     CDS             complement(28966..29583)
                     /gene="dgoA"
                     /locus_tag="ACHX5T_000656"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR8465979.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(29567..30445)
                     /gene="dgoK"
                     /locus_tag="ACHX5T_000657"
     CDS             complement(29567..30445)
                     /gene="dgoK"
                     /locus_tag="ACHX5T_000657"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR8465980.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(30442..31131)
                     /gene="dgoR"
                     /locus_tag="ACHX5T_000658"
     CDS             complement(30442..31131)
                     /gene="dgoR"
                     /locus_tag="ACHX5T_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR8465981.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            31409..32065
                     /gene="yidX"
                     /locus_tag="ACHX5T_000659"
     CDS             31409..32065
                     /gene="yidX"
                     /locus_tag="ACHX5T_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFR8465982.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(32111..32923)
                     /gene="yidA"
                     /locus_tag="ACHX5T_000660"
     CDS             complement(32111..32923)
                     /gene="yidA"
                     /locus_tag="ACHX5T_000660"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR8465983.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(33037..33435)
                     /locus_tag="ACHX5T_000661"
     CDS             complement(33037..33435)
                     /locus_tag="ACHX5T_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR8465984.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(33675..36089)
                     /gene="gyrB"
                     /locus_tag="ACHX5T_000662"
     CDS             complement(33675..36089)
                     /gene="gyrB"
                     /locus_tag="ACHX5T_000662"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR8465985.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(36118..37191)
                     /gene="recF"
                     /locus_tag="ACHX5T_000663"
     CDS             complement(36118..37191)
                     /gene="recF"
                     /locus_tag="ACHX5T_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR8465986.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(37191..38291)
                     /gene="dnaN"
                     /locus_tag="ACHX5T_000664"
     CDS             complement(37191..38291)
                     /gene="dnaN"
                     /locus_tag="ACHX5T_000664"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR8465987.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(38296..39699)
                     /gene="dnaA"
                     /locus_tag="ACHX5T_000665"
     CDS             complement(38296..39699)
                     /gene="dnaA"
                     /locus_tag="ACHX5T_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR8465988.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            39996..40076
                     /locus_tag="ACHX5T_000666"
     CDS             39996..40076
                     /locus_tag="ACHX5T_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8465989.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            40306..40446
                     /gene="rpmH"
                     /locus_tag="ACHX5T_000667"
     CDS             40306..40446
                     /gene="rpmH"
                     /locus_tag="ACHX5T_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR8465990.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            40463..40822
                     /gene="rnpA"
                     /locus_tag="ACHX5T_000668"
     CDS             40463..40822
                     /gene="rnpA"
                     /locus_tag="ACHX5T_000668"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR8465991.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            40786..41043
                     /gene="yidD"
                     /locus_tag="ACHX5T_000669"
     CDS             40786..41043
                     /gene="yidD"
                     /locus_tag="ACHX5T_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR8465992.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            41046..42692
                     /gene="yidC"
                     /locus_tag="ACHX5T_000670"
     CDS             41046..42692
                     /gene="yidC"
                     /locus_tag="ACHX5T_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR8465993.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            42798..44162
                     /gene="mnmE"
                     /locus_tag="ACHX5T_000671"
     CDS             42798..44162
                     /gene="mnmE"
                     /locus_tag="ACHX5T_000671"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR8465994.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        10699 a        12007 c        11339 g        10188 t
ORIGIN      
        1 attagccggg ctgaatacca ggaattgtta gccgtttccc gtggcgacgc tgttgccgat
       61 tatattattg ataatgtctc tattctcgac ctgatcaatg ccggagaaat ttccggccca
      121 attgtgatta aaggacgtta cattgccggt gttggcgcag aatacgctga tactccggct
      181 ttgcagcgga ttgatgctcg cggcgcaacg gcggtgccag ggtttattga tgctcacctg
      241 catattgaat ccagcatgat gacgccggtc acttttgaaa ccgctaccct gccgcgcggc
      301 ctgacgaccg ttatttgcga ccctcatgaa atcgtcaacg tgatgggaga agccggattc
      361 gcctggtttg cccgctgtgc cgaacaggca aggcaaaacc agtacttaca ggtcagctct
      421 tgcgtgcccg ctctggaagg ctgcgatgtt aacggtgcca gttttaccct tgaacagatg
      481 ctcgcctggc gggaccatcc gcaggttacc ggccttgcag aaatgatgga ctaccctggc
      541 gtaattaacg ggcagaatgc gctgctcgat aaactcgatg catttcgcta cctgacgctg
      601 gacggtcact gtccgggttt gggtggcaaa gaacttaacg cctatattgc tgcgggtatt
      661 gaaaactgcc acgaaagtta tcagctggaa gaaggacgcc ggaaattaca actcggcatg
      721 tcgttgatga tccgcgaagg gtccgctgcc agcaatctca acgcactggc accgttgatc
      781 aacgaattta acagcccgca atgcatgctc tgtaccgatg accgtaaccc gtgggagatc
      841 gcccatgaag ggcatatcga tgccttaatt cgccgcctga tcgaacaaca caatgtgccg
      901 ctgcatgtgg catatcgcgt cgccagctgg tcgacggcgc gccactttgg tctgaatcac
      961 ctcggcttac tggcacccgg caagcaggcc gatatcgtcc tgttgagcga tgcgcgtaag
     1021 gtcacggtgc agcaggtact ggtgaaaggc gaaccgattg atgcgcaaac cttacaggcg
     1081 gaagagtcgg cgagactggc gctgtctgcc ccgccatatg gcaataccat tgcccgccag
     1141 ccagtttccg ccagcgactt tgccctgcaa ttcacgcccg gaaaacgcta tcgggtcatt
     1201 gacgtcatcc ataacgaatt gattacgcac tcccgctcca gcgtctacag cgaaaatggt
     1261 tttgatcgcg atgatgtgtg ctttattgcc gtacttgagc gttacgggca acggctggct
     1321 ccggcctgtg gtttgctcgg cggctttggc ctgaatgaag gcgcgctggc tgcgacagtc
     1381 agccatgaca gccataatat tgtggtgatc ggtcgcagtg ccgaagagat ggcgctggcg
     1441 gtcaatcagg tgattcagga tggcggcggg ctgtgcgtgg tacgtaacgg ccaagtacaa
     1501 agtcatctgc cgttacccat tgccgggctg atgagcaccg acacggcgca gtcgctggcg
     1561 gaacaaattg acgccttgaa agccgccgcc cgtgaatgcg gtccgttacc cgatgagccg
     1621 tttattcaga tggcgtttct ttctctgcca gtgatccccg cgctaaaact aaccagtcag
     1681 gggctatttg atggcgagaa gtttgccttc actacgctgg aagtcacgga ataataaaaa
     1741 aagcccggcg tcatgccggg caaaagtcac cagttacgtt tatgccactg tcaactgctg
     1801 aatttttttc tcgcggcgga ttttgcgttc ttccattacc gccactatcg ccatcaggca
     1861 gatacaacca atcgcggcga tatccagcgc ggcgaaggtg cctgcccagc cggtaaggcc
     1921 gaataccggc gtcccatcgg caatcattcc cagacctaac ttggcaaagc tgtcaccaat
     1981 caggtaagca aaggtgcctt taataccatc ggcagcgcca atcgcttttt taggtacaaa
     2041 gccaacagca gccacaccaa tcaacaattg cgggccaaag accaggaaac ccaacgcaaa
     2101 gagagaagcc agatagatat attcgttact ggcatgttga tacacaccga gcgtggcgat
     2161 aatcagcgcc agcgcgatgc aggccaccag gccacggcga ccgttcgcca ggtcagagag
     2221 ccagccccac agcagcgtac cgaccagcgc accagcttca aacagcgtaa agccctgaat
     2281 cgccaccgct ttagagagtt tcagttcctg gaacgcgtat acggttgacc actggtcgat
     2341 accaatacgt accacataga ggaaaatgtt ggcgaagcac agcagccaga tcactttgtt
     2401 tttcagcaca tactcaacaa agatctgcca cttggtcata tcggtagatt ctgtctcttt
     2461 gtcctcttcg ctgatctcct cgccgaacag ttcttcagct ttgccgaggc cataagattc
     2521 cggggagtcg ctgccgtaac gcaggccgat aaaaccgaca atcagcgcga taatcgacgg
     2581 gaagataaac atgccgatga catggccatc gaacaggtaa tttgccccga acagcgccac
     2641 acctgctgcg cctgcaccgc caaggttgtg agaaatattc cagaaaccga ggaatgtccc
     2701 gcgtttacga cgcggcgtcc atttggtgat ggtggagtaa ctgcacgaac cgccggtact
     2761 ctggaaaaag ccgcttaagg cgtagaaggc aatcatcagg aacaggctaa ccgagccgct
     2821 gcccatactg gcactgaagc ccagcataca aatggcagag aggataagca tgaacggcag
     2881 gaattgtttg gtgtttttgc cgtcggcgta gtatgaaacc aacgttttac ccacgccata
     2941 agtgatggag aaacccaggc cgatcatccc cagctgcgtc atgctcaacc cgtaggtcga
     3001 aatcatatcg ttctgcgcga tgttaaagtt cttgcgaatc aggtacatcg tcaggtagcc
     3061 gataaagacc accaggtagg attgcatgaa cggtttgaac cacattttgc gccgcacttc
     3121 gagcggaagg tccagggtcg gcttgcgaac ttggtttagg aaagccagca tgggttactc
     3181 ctgaaatgaa tacctgcgtg aggcatgcat tgtaaaaatc agcagcaaat caggcctgag
     3241 atagcgtcca agaaaaacct ggaaaatttc ttagttttgc cggatatgaa gtgaaaaggt
     3301 gagatgcatc acgcttcacg cggtgtctgg gcgttcaaaa agggcagtaa cagcagtgcg
     3361 gaaatcccgg cggcgataga gataaccaca aaaaatccgc tccagtgcca ggtatcgagc
     3421 actttcgcca gcggccaacc agcaagcgac gcccccagat aagcaaacaa gccgacaaac
     3481 cccgtcgccg cccctgccgc ctctttgtgg gaacactctg ccgccgccat accaattaac
     3541 atctgcgggc caaagacaaa aaaaccaatg gtgaagaagc aggttgcctg catcacgtag
     3601 ctggcaaatg gcatcagcca cagcgagccg actgaaagca aaattccggc ggcgaaaatc
     3661 aaattcatcg gcccacggtt gccgttaaac aatttgtccg agccccaacc ggctaccagc
     3721 gcaccgataa atccgcccag ttcaaacatc gtcactgccg tattcgccgt gaccagatcg
     3781 acgcccagcg tctcggacat atacaaattg ccccagtcgt tgatcgccgc ccggaccaca
     3841 tagaccagca catagcaaaa cgaaagcagc cagatatacg gattcagcaa cacatatttg
     3901 gtgaggatct ctttacgcgt caaccctgcc ccttcttgtt gttgagcaat ttccagcgcg
     3961 tcgtgccgcc attcaccgac cgccggtaaa cctaacgcct gcgggcgatc gcgtagccgc
     4021 cagcagagaa aaatccccac gactatcgcc atacaaccag caatcatcat cccggcacgc
     4081 cagccgtaat gcagcgcgct cgctgccatc acaatgggaa tgagtgcgcc gccgacgtta
     4141 tgcgccgtgt tccataatgc ccaccaaccg ccgcgctcgg tacgtgaata ccaggccgtt
     4201 aacagacgcg cacacaccgg tgaaccccag ccctggaaaa aggcgttcag cacccagagc
     4261 acggcaaacg cccatagcga cgtcgagaag ccaaacagaa tgttcatgat gcccgtggcg
     4321 ataagcccta tccccataaa ataacgggca tttgagcgat cgctgacaat gccggagaca
     4381 aacttcgaca cgccgtaagt aatgtaaaac agggtcgcta acaggccgat atcgctacgg
     4441 ctgagcacgc cgttagcaag gatttctggt accgcggcgt taaaactttt ccgcgtgaag
     4501 taaaacagcg cgtaacccag ccagatggtc agcagaatat gccgacgcca gtagcgataa
     4561 cgggcatcaa tttcatgttt atcagtcatt aatggcgcat cggcaggcgc tttcagaaac
     4621 ggcaacatca tcgaacctta gacataacgt tgaggtagag aaacgctgac acgcgtgccg
     4681 tgcagacagg aaatgtgtaa tgtgccgccc agcgccgtta cgcgctcgcg cattccggtg
     4741 aggccaaaac cttgttgccc ggaacccggc ggcaaaccgc tgccatcgtc ttcaatcacc
     4801 agcatcaacc gttcatcctg ctgccagcct tgcagcgtaa ccgcgctggc atcggcatgt
     4861 ttcacaatgt tgttcagccc ttcctggcag acacgaaaca gcgtcacgcg ctggttttcg
     4921 cttaatgctg attcatcgat acgccattcg agatggctga caatgccgcg cccttccagc
     4981 tccatttccc gcatcagtga gcggatggcc tgctccaggg tgagatcatc cagctggcgc
     5041 ggacgtaacc gtcctaacaa acggcgcacc gcgtcgtaaa cgcccagcga cagttgttcg
     5101 atgagctgcc cgctctgctt cacactggcg ttatctgccg ccagccgctg aacaatgccc
     5161 gcctgagtac gaatagcagt gatggtctga ccgatatcgt cgtgcagctc acgcgccacg
     5221 tcgcggcgca cgctctcttc ggtttctaac aaacgttcag ccagatgctg attgcgcgcc
     5281 agttcctttt gtagcgactg gttaagttca cgcaaccgct ggatgcctgc gcccagtaac
     5341 aaccctgtca gactttgcac cagcaacgag agtaataaat ccaccagatg atcgcgccag
     5401 gtttgactgg cgatcagcgc gatggcgttc atcaacgtcg caatcagcgc tccttgccag
     5461 ccatagtgcc aggccagcgc gataatcggc agcgccagac agaatggcgt aaagcgcgac
     5521 agttcgtccg gcaatcccaa ctggagccag agactgataa caaacagcag caagtaccag
     5581 accagatgcc gcccgcgcca gttgatgggc tgagaaacca gtgacgggcc gagcggcagc
     5641 caggtgttat tggcgagata gtgccagaac accagacata tcggggccag cgtcagaccg
     5701 ccagttaaag tcagcaacag cgcattccac gactctttgc cgtgccaaag ccagggcagc
     5761 gactgcaaca acgccgccgc cgttaacgcc gcaccctgta acagcaaggt gcgccagtca
     5821 cgctgatggc gatagcgcga gatcagcgct acgggcagta acgtcagcaa actaccgatc
     5881 atcaataacg gaaaatgggt taaaccgacc gcctgcgtta gccagtaaat cagcagccac
     5941 tccgcaccca gcaatacggg ccagtatccg cgcgggcatt gcagcattag ccccagacgc
     6001 agaccaaacg gaaataacag caccgccata tcagggcgct caaccagatg caggctgata
     6061 ctccataggc aaaaccatgc ggcagagaag ataaaaaagc aggcaataac ggtaattaag
     6121 cgggagaaca acgtcttcat caccagccat caaacatgcg gcgcgccagc tctacgtcgt
     6181 tactgacgcc cagtttttcc atcagattgg cgcgatggac gtgtaccgtt ttcggtgaca
     6241 agcccagttc ggcggcaatc tctttcaccg ccattccttg cgccagtttt tccgccacct
     6301 ggcgttcacg tttggtcagc gggtcctgac gaccggatgc cagtttaatg gcaatatccg
     6361 gcgtcagata acagccgccc gtggcaaccg tatgcaccgc agcaatgagt tcatccgggc
     6421 tacagcgttt ggaaagaaag ccgcgtgccc ccgcgttaag cgcctgctca accagcgcag
     6481 gactgtcgtg aacggagagc ataatcgtcg ccataccttt cggcagctgg cttagcagct
     6541 ccagaccgga gatatcgggc atggagatat cgcaaataca cacctgcaca ccgcgccccg
     6601 gcagccccgc cagcgcctcg cgccccgaac caaactcggc aactacctgc aaatcaggtt
     6661 ccagccccag cagctgcgca aagccggagc ggacgatgag gtgatcgtct ataagggcaa
     6721 cggtgatcat ggtcttgtcc tggcgggtaa aaaaaaacgc gcttacctta acgataagcg
     6781 cgatgttgtt caagccttga gcggttactg aaaaaacacc gcgatcttgt taaacatcgt
     6841 cggatcggac tgattacgct gcactttcac gacatcttcc agcttagcga tttggcttat
     6901 catctgctcc agacgctggt cgtcattgac cagtagccag atatggcttt tgtcgctgtc
     6961 ctgaatcggc agacaaagaa tgccttcaac gttaaaagcg cggcgggcaa aaaggccaca
     7021 aacgtgggtc attacgcccg gatggttgcg aacggtgagc tccagaatta cgttgttatg
     7081 agttgtgttt tgcatggctt attcccccac catttcagta ttcgccgcac ctggcggcac
     7141 catcgggtaa actttttctt cggcatcaat gcgcacatgg atcagcgccg ggccagggcg
     7201 attgatgatt tcctgcaatg cagcctgcgg atcggcttcg ttattcaaat cacaggtttc
     7261 gaggccgaat ccggcggcaa tctgcataaa gttgattttg cccggatagg tggcggcaaa
     7321 aacgccttgc tcgtagaaca gactctgttg ctgatgcacc agccccagcg cttcgttgtt
     7381 catcagaatg attttgacat ccagctgatt ttcactggcg gtcgccatct cctgaatatt
     7441 catcatcagg ctgccgtcgc cggagaaaca caacactttg cgatccgggt tcgccagcgc
     7501 cgcgccaatc gccgcaggca ggccaaaacc catcgtgccc agcccaccgg aggtcagcca
     7561 ctggcgtggg cgattgagcg gataagcttg cgcggtccac atctgatgct gacccacatc
     7621 ggtggtgata atcgcattgt catcgacaca ggcggcaacg gcgttgatca ggccgtaatg
     7681 gcttaatgga tcgcacgctt tcgggattgg acacgggaat tcgcgctgca aatccgctac
     7741 caactggtgc cactctgcac gcggttgcgc ttccacccgc gggatcaatt gcgccagcac
     7801 gtcatcaaca tccgcctgaa tcgccacatg cggctgcttg attttaccca gctctgcgcg
     7861 atcgatatcg acatgaataa ttttggcatt cggacagaac tgctcggttt tgccaatcgc
     7921 ccggtcatca aaacgcgcac cgagcacaat caacaaatcc gcctcctgca aaatatagtt
     7981 ggtactgcgc acgccgtgca tccccagcat acccagcgac aacggatgcg cttttggcag
     8041 catgcccagc gccattaaag tcatggtggt aggcagttgc gctttctccg ccagttcacg
     8101 cacccgcgca ggcgcattga tcacaccacc acccagataa agcaccgggc gtttggcagc
     8161 gttaatcatt gtagctgcgt cacgaatgct ttcttcgcta aaggcgggtg caacggcttt
     8221 ttctgccacc gcgggctgag cttcaatctc aaaaaccgcc gtttgtacat ccttaggaat
     8281 gtctatccac accgggctag ggcggcctga ttgcgcaatg cggaaggcat cgctcatgac
     8341 ctgtgggagc tcttcgatat gtctgaccag atagttgtgt ttggtgatgg ggatagagat
     8401 gccgtaggtg tccacttctt ggaaggcgtc ggtgccgatc atcgaggcgg gaacctgacc
     8461 agtgatgcaa atcagcggga tggagtccag ccgcgcatca gcaatggcgg tcaccaggtt
     8521 agtcgcaccc ggcccgctac aggccataca gaccgccggt ttaccgtcgg tgcgcgccat
     8581 tccctgagcg ataaaccccg cgccctgttc atggcgagcc agaatatggc ggatttgcgt
     8641 gctttggctt aaggcgtcgt aaacaggcag gatagaaccg cccggaatgc ccgtcacaat
     8701 cttaatgccc tgctgttcca ggaaatgaac gataaattct gcgccggtaa agcgcttacg
     8761 cgtcgatgtt gtgcccgaac ttgccatgct ccagtccttt tcttctgggc cgactttccg
     8821 ggaggtgctt aaacgaaaaa ccccgcccgg tttgcgccgg cggggttttg gaatcgtgtg
     8881 ttgttccagt ccctacggcg cattgccgac gaccaccacc acacgcacga cgaccactgc
     8941 ggcggatggc gcagttggta gtagttttgc gttgaacatg gaagtagtca ttggggacct
     9001 tgttggtttt gtgtttaaca atatttatac aagcacagct ttacagggga gacaatggaa
     9061 aatttttcag caagggaaaa ttgaggggtt gatcacgttt tgtactgaat tgcagataac
     9121 aaaaaacccc gccggagcga ggtttcgtca gccgcctgcg gctggtaacc gcaaagcaca
     9181 ctgtattatg tcaacactga aagtatacgt gttccgcgca gaacgcgcaa tttcggcacg
     9241 aattttgacg tatttagtgc atagttgagt atcgttcaca gtttgcgttt tgtccaaata
     9301 ttactgttta tttatacagt aagcttctat aatatcactg tacgcaatgt gttatgcggg
     9361 ggccgcatcg ttacccggcg cactaagtcc tggctgaaac gggtggtgcc gtcagcgcct
     9421 taaccccgcg tgagcacact gtgttatgtc aacaagcaca acgtttctcc ttgagatacc
     9481 gcgtgcacaa cagctggcaa caggcagcgg aaaggtacgt cagctggcag tgctcctgaa
     9541 ccacaggaga cgcgtatgag cctggtggat atcgccattc ttatcctcaa actcattgtt
     9601 gcagcactgc aactgcttga tgctgttctg aaatacctga agtaattcag attcaagtcg
     9661 caccaaaggg gagcgggtaa ccgctcccct tttatattta gcgtgcgggt tggagtcgga
     9721 tgcgatgctg acgcatctta tccgccctac catccctccc ggcaacattt attgccgctt
     9781 ttgtttacat attctgccgc taaacaattc cccattcctg gcgtatatct ggctaacatt
     9841 catcaatgtg atagattcct ctcccgcatt tatgggaatg cgtagtgact tattctaatt
     9901 atttttataa aagcatccgt gataatgaaa aggcaaagaa acgtcaattt gttattgatg
     9961 ttggtattac tcgtggccgt cggtcagatg gcgcaaacca tttatattcc agctattgcc
    10021 gatatggcgc gcgatctcaa cgtccgtgaa ggggcggtgc agagcgtaat gggcgcttat
    10081 ctgctgactt acggtgtctc acagctgttt tatggcccga tatccgaccg tgtcggtcgc
    10141 cgaccagtga tcctcgtcgg aatgtccatt tttatgctgg caacgctggt cgcggtcacg
    10201 acctccagtt tgacggtatt gattgccgcc agcgcgatgc aggggatggg caccggcgtt
    10261 ggcggcgtaa tggcgcgtac tttgccgcgt gatttatatg aacggacaca gttgcgccat
    10321 gctaacagcc tgttaaacat ggggattctc gtcagtccgt tgctcgcacc gctaatcggc
    10381 ggtctgctgg atacgatgtg gaactggcgc gcctgttatc tctttttgtt ggttctttgt
    10441 gctggtgtga ccttcagtat ggcccgctgg atgccggaaa cgcgtccggt cgatgcaccg
    10501 cgcacgcgcc tgcttaccag ttataaaacg cttttcggta acagcggttt taactgttat
    10561 ttgctgatgc tgattggcgg tctggccggg attgctgcct ttgaagcctg ctccggcgtg
    10621 ctgatgggcg cggtgttagg gctgagcagt atgacggtca gtattttgtt tattctgccg
    10681 attccggcgg cattttttgg ggcatggttt gccgggcgtc ctaataaacg cttctcaacg
    10741 ttgatgtggc agtcggttat ctgctgcctg ctggctggct tgctgatgtg gatccccgac
    10801 tggtttggcg tgatgaatgt ctggacgctg ctcgttcccg ccgcgctgtt ctttttcggt
    10861 gccgggatgc tgtttccgct ggcgaccagc ggcgcgatgg agccgttccc cttcctggcg
    10921 ggcacggctg gtgcgctggt cggcggtctg caaaacattg gttccggcgt gctggcgtcg
    10981 ctctctgcga tgttgccgca aaccggtcag ggcagcctgg ggttgttgat gaccttaatg
    11041 ggattgttga tcgtgctgtg ctggctgccg ctggcgacgc ggatgtcgca tcaggggcag
    11101 cccgtttaag cgcacgtcac cgcagcatcg tcatcagctc catgggagaa cgatgctgct
    11161 ttatcagatc acgcatcacc cgcatatgcg gtgcggagta agaataaaac gcctgatagc
    11221 ccgcacaaag cacgctgtct ggcgtctcct gccgagcgtg cgggcacagg cgatgccagt
    11281 cgcacgcctg gcagtcacgg cgcggttgcg ggttttcgct gccacaccat ttttgtaagg
    11341 tggcgtcaaa aagctcaatc gagatacgac tgatatctgc ccgcacccaa cactcaaaca
    11401 gggcaattaa aaatttcccc cacagctctg ctgtcaccga agcggccgtc agatcgcccc
    11461 ggtcatcaaa ttcaagatag ggaatgagct gtacggagcg gatgccccgc tgtttgaaat
    11521 tgtgataaaa atcggcaggt tgcagagcat ccttgtactc tacaaccagc ttatgcctgt
    11581 cctcttcagc atcaatgacc tgacttcctg tcatgcgact ctctcaataa atacgataag
    11641 ttgatgtata tgcgttacag caaacagtat tgcgagagat aacaccgcga gggagatcaa
    11701 aaatttgtca cggactacgt gaaattggga aaaatcgcta ttcgtgacat ccattaaatt
    11761 gcgattacgg gtgtagtgcc agaggatcag cgccacgatg gcgaggatgc caatcgaaat
    11821 ccagaataac atgcccgcct ggtgccagtt gtgtttgata gccaacgcca tcaacgcgcc
    11881 gtagcccagc atggtacgaa accacgccag tgatgtgcgc tccggctgca gccctgggtc
    11941 ggcaatgcgt ctggctttac ggctatccgg catacaacac cagtcccata acgatcaccg
    12001 ccacaaccat caatattaag ctgatgatta ataagctgtt ggtgtagggc aggtcctctt
    12061 tcagacgcat cgctttttca ttgcgtaacc agcgcaggta gccatacatt gctaacccgc
    12121 cggaaaacag acacaacaac aacgccagca gttcacgaat gacgggcgtg gcgaaatctg
    12181 gcgcaagttg atcaagccca acccccgccg ccagaaatcc tagtgcggta cggatccacg
    12241 ccagaaaagt acgctcatta gccagcgaga agcggtaatc gggcgcttct ccaaggcggg
    12301 aaattttcat tgttgctcct ttgcaatttc actaacgctc tatctttagc ggtttttttt
    12361 aagaatttcg tccgcccgtt tcctgatatg cttttgccat ccgctacaag gaggtcagat
    12421 gggtatcata gcgcaaaata aaatcagttc attgggcatg ctgttcggtg ccatcgcttt
    12481 aatgatgggg attatccact tttcattcgg ccctttttcc gcaccaccgc caacgcttga
    12541 aagcatcgtg gcggacaaaa cggctgaaat taaaagaggt cttctcgccg gaataaaagg
    12601 cgaaaaaatt accactgtgg agaaaaaaga ggacatggat atcgataaaa tcctcgatca
    12661 aagcggtatc gcactggcca ttgctgcgct actttgcgca tttattggcg gtatgcgtaa
    12721 ggaaaatcgc tggggcattc gcggcgcgct ggtttttggt atcggcatcg tgtgcagcat
    12781 tttgctgata ttcctgattt tttccttcct gaccggcggt tcgctggttt aagcagcctg
    12841 aataatgccc gccgggtgat catcagcggg cattatcgtc agaatttctg ttttttctcc
    12901 tccaccttca ctccttgcgt cggtaggccg gtgtcatagt cgcgtaccac tggcgaatag
    12961 ccatcttgtg ggcgtgggcg aaacgccccc atccagcgcg gtcgtgcatc tttacgccac
    13021 ggacgcagac tccattggta actgcggaac ggatcgcgaa ttttgtccat gtaatccaat
    13081 aaggcgtcat gcattttgct gcgaacgtct gcaaaacgga tatcatcgat caggttatgc
    13141 atttcatttg ggtcattacg gcgatcgtaa agttcatcac tggtgaagag gttgagcacc
    13201 agtttaaagt catccgtcac ccagcaacgc accggaataa aaccgccaaa gctgtcatgc
    13261 tcaatctcgt agcggttaaa ttccaccatc acgccgcgtg gctctttcac ggcaaggata
    13321 ttttcccccg gcagaatctc tggtttttca atatctgcca gcgccatcat tgtcggcagt
    13381 aaatcaatat gactgactgg cgtatcgacc tgtcgccgct ccccttgcgg cgaacggatg
    13441 atcagcggaa tacgggtgat gtcgtcatac atcgccgctc ctttactgat cagcttatgt
    13501 gcgcccatca tttcaccgtg atcggaggta taaatgaccc acgtattttc acgttgctct
    13561 ggcgttaagg cattgatgac acgtccgatt tggtcatcaa caaagtcatt gcaggcaaaa
    13621 tagagcggat ggtgataaag cccgtcatca ccgactggcg atggcatcgc ctgcgcccat
    13681 aagcggtgat gttccggttt gttcgccagg tcatcctgtg atttctcgcc aagttcgtag
    13741 taaaaatcag cgtatttctc taaatactcc accggacagg tgaacgggtg atgcggctca
    13801 tcatacgaaa ccaccatcag gaagggttcc tcggcgcgca cgggctattg cagaaaatct
    13861 accgctcgat tgctgatgcg gtgcgcccag gtgaaggttt cgtcaatatg gttcgcctgt
    13921 aaatcttcga cgctgtttag gccattgcgc cacaggctga tctctttttc cgtcagttcg
    13981 ctaagatagt tcgccccatc aaaccagtaa tcagcatccc actccggcgg acactcgcca
    14041 gtgccgaaat agtcatgacc gtcgagatgc catttgccga tgtaacaggt gtgatagccg
    14101 gcatccttaa agtagcgccc catagtggag atgtttttgc ctggcgcgac gttgttggtc
    14161 cacgggccgg actggttagc gtagataccg gtgaatagtc cggcccgtgc aggcgtacaa
    14221 accggtgaac aagtgtaggc ggaattaaag cgaatacctt ccgccgccag actatcaata
    14281 ttttgcgtat tcagcggttt accgctatag caaccgacca tattggtggc ctgggtatcg
    14341 gtcaattggg gcgtttcata ggctatcctt cgcgtcggga gtaaaggtta cggctgggtc
    14401 gtgacgatgc caccagctgt caaaaatcag gtagacaatc actacggcag cgatgaaata
    14461 actgatcatc gctaaccagc gcgtaccgta gccgccgaat tcagccagcc cgacatagac
    14521 gccaatcatc gcgaacagga taccaattga cgcgatcttg acgtttttcc acggtttcat
    14581 gtccaccgca aacgcatctt tgaaggtgaa cggcgtggcg cgcggtttga taaaaccgat
    14641 caccagcatc acgaccacgt tgatgcagaa cgtacaggcc agcacataga ggaaatggaa
    14701 gtcgaacttc accagatagt tgatggtgat gtagctgatt atgccaatcc ccatcgccac
    14761 ttttgccgcc agcgccggga tgcgcggaaa gaaaaagccc ataatgatga tggtaaccag
    14821 cggcacgttg taaatgccgt tgagctgttt catccagcta tacagcccct gcggcgcgtt
    14881 cgcgatccac ggcgctacca aaaccgaaac gatagcgata aagaaaccaa atttgcgccc
    14941 gacggtgacc agctgctgcg gctcggcatt ctggttaatg atgcgacggt aaatacccat
    15001 actgaataat gtactggcgc tattcagaaa gccgttgaag gtactgatca ccgcaccaaa
    15061 taacaccgcg ccgaagaaac cctccagtgg cactggcaga acgttattga ccagcgtcgg
    15121 gtaggccatg tcggctttcg gtagatcctg atacagatga aacgcaatca accctggcag
    15181 caccagtacc agcgggtcga gcattttcag caccgccgtt aacagcgccc ccttttgccc
    15241 ttccgccagg ctttttgacg ccagcgtgcg ttgcacgatg ccctgatttg tacaccagta
    15301 aaaggtgttc accagaatta aaccggtaaa tgccgcgcca atcggcaagg gatcggtcgg
    15361 gccaccgact gagtttaatt tctcggcgtg aacggtggta atttgctcaa tgccctgcat
    15421 aaagctgccc ttgcctatcg cgatcaggcc aaataccggc accatcaacc cgccgataac
    15481 cagcccaata ccgttgatgg agtccgccac tgccattgcg cgcagtccgc cgatcaccgc
    15541 atacaaaatt cccgccagac caagcaaaat taccagtagc cagatagccg caccgtgaga
    15601 aatctgtagc gattccccga cgtgaaacag gctgttgagc gccaacgcgc cggagtagag
    15661 aacaatcggc agaaagcaga cgccggtggc aatgaggaag cagaagtcga tgataatacg
    15721 cgtcgtttta tcataacgtt cctccagaaa atcggggatg gtggcaatgc cgcgcttcag
    15781 atagcgcggt aaaaagatta gcgcgaggaa gatcagcgtc accgctgaag tcacttccca
    15841 gcccatcacc gacatgccgc ttttgtaggc ctgcccggaa agaccaacca gttgttccgt
    15901 ggaaaggttg gttaacatta acgaagcggc aataaccggc gcttttagtg aacgtccggc
    15961 aagaaaatag ccttgttgcg atccggtatc tgttttgcga accttccacc aggtgatcac
    16021 cgccaccagc agcgtaaaac cgacaaaact caagatttgt aacgaattca tctcatagcc
    16081 cttatttatg tttatgtccc ggacgcatga atatagggag aggtaaaatg cgtcgggtca
    16141 ggcagtcaga ttcttaaaat acaaacgtcg tatccctgaa cggattgttt tctgttaagt
    16201 tcaggttgtg agcataattc gccggaggga tattaaaatg aatggaaaat tgcaaagttc
    16261 agatgtaaaa aacgaaactc cgtacaatat tcccttattg attaacgaaa atgtgatctc
    16321 cagcggaatt tctctgatct cgctctggca tacctacgcc gacgagcatt accgggtgat
    16381 ctggccgcgg gacaagaaga aaccgcttat cgccaactca tgggttgcgg tgtataccgt
    16441 acaaggatgt gggaaaattc ttttaaagaa tggcgaacaa ataactctgc atagcaactg
    16501 tattatattt ttaaagccaa tggatattca ctcttatcac tgtgaaggtt tagtctggga
    16561 acagtactgg atggaattta cccccaccag tatgatggat atttccgttg gtcagcaaag
    16621 cgttatttat aatggcgaaa tttataatca ggaactcacc gaagttgctg agttaataac
    16681 ttcaccagaa gcaataaaaa ataatctggc agtcgctttt ctgacgaaaa ttatttatca
    16741 gtggatttgt cttatgtccg cagatggtaa aaaagatcca caacggcggc aaattgaaaa
    16801 attaattgcc actttacatg ccagtctgca acaacgctgg agcgtagctg atatggctgc
    16861 cacgatcccc tgtagcgaag cctggttgcg tcgtctgttt ttacgctata ccggcaagac
    16921 gccgaaagaa tattacctcg atgcgcgtct ggatctggcg ctatcgctat taaagcaaca
    16981 aggaaactcg gttggcgaag tcgctgatac gctcaacttc ttcgactcct ttcatttcag
    17041 caaagccttt aaacataaat ttggttatgc gccgtcagcc gtgctaaaga atacggacca
    17101 gcacccaacg gatgccagtc cacacaatta acgtaactct ggccagtacg ctttgttggc
    17161 ctccagcaac tcatccagaa tagctttggc gaccgaggcg ctcggtaccg ttttcgacaa
    17221 cgtaatcgcc tgccacaggt gctgatatga acgctgttcc caggcgtcca cgaccagttt
    17281 ttccaccgcc acttgctgac tcattaaccc tttctgaaac tgcgggatat cgccgaccac
    17341 cagtggttca ggccgtagta gcgcacgcgc atctgtttgc gatcttgcag cccaacaatt
    17401 tgcgccatcc ggctttcaat accgattggc atgtcacaga tgttgaggat tttcgcattc
    17461 gggcgcagac gacgcgtggc ttctgcgaca attgctgccg ggttggagta gttgagcatc
    17521 taggcgtttg gtgaatattt ttccatataa tccaccagtt ccaggacgcc gccaatggaa
    17581 cgcatgccgt acgctattcc gccgggtccg caagtttcct gaccaacaac accgtggcgc
    17641 agtgggattt tttcatccag ttcgcgcatc gggtacttgc cgacgcggat gtgcgccatg
    17701 acaaaatcaa cgtcgctgaa cgccacttca ggatcggtgg tgtaactaaa cgcaatgtcc
    17761 ggcgcttttt ctttaaggat gactttacag gcttcggcaa tcacttcctg ccgcgcgcca
    17821 tcgttatcat aaaatttcag tgcacgaagc gggaaacggt cctgattcgc caggagcatc
    17881 aacacgatgc ctggcgtaaa ggtgcttcca ccgcctgcga caaccactga gaatttggtc
    17941 attatactgc ctccgtaatg gcaacatttt ctgctgattg atgagaatta attaagctat
    18001 cgagctgttc acgcaattgg gatacatgca gaccaatgat cacctgaatg gcatcgccac
    18061 tacggaagac gccgtgcgct cccagctttt taaagacttc gtcatccagc gtttgtgaca
    18121 tgtcatgcag tgcaatacgt agacgcgtcg cgcaattgtt aatgctggag atattgccga
    18181 ccccgcccag ggcttgcagg ataccggcag cctgatccag ctcttttttt ggctctgcag
    18241 cggtggtttg gcctcgcgag gctttgtatt cggcttttga gtagagtttc acttccgcat
    18301 cttcacgtcc cggcgtgcac atgttgaact gcagaatcag ggtgcggaaa accacgaagt
    18361 acagcagggt aaagcacaac ccaatggcga tttgggtcag catcatatcc gcgtggttgc
    18421 tgaacatcgg gatccagttt tgcggtaaaa cctggtcaat cagacctccg cccatgttgc
    18481 ccaccacacc aaagagatac attacggtcg acattgaggc cgccagcacg gcgtgtaccg
    18541 caaacagcaa cggtgaaatg aacaggaagg taaattccag cggttcggta attcccacca
    18601 gcatggcggt taaggttgcg ggaatcagca agcccgccac ttttacccga ttttccggtg
    18661 cggcagtgaa gtacatcgct aaagaaatgc ccacggcacc aaagattttt gagttaccgt
    18721 gcagggcaaa accgccttcc gggaacaacg atttcagtgg ctcggcgctc agactgaact
    18781 cttgcagatg ctgcgcccag tacatctgaa tgccgccttc aacagctgcc ggaccaaaga
    18841 taaacggtcc gtagatgaag tggtgtaaac cggttgggat cagaatacgt tcgaggaagg
    18901 tgtaaaccca caccccaagc gcacccgccg aacgcaggaa cgcttgcaga gattcaatcc
    18961 ccatttgtac ttttggccag ccgagcaatg tcagccaggc acaggggatc atcaccagga
    19021 aggcgataat caccacataa gacgtcccct ggaaaatgcc gagaaaaacc ggcagttttt
    19081 tatcgaacag acggttatgc agcgccgtca caatgccgga aatgataatt gcgccgataa
    19141 tgctggtatc gagggtttta atcccggcca tcattgtcag accgctacct gccaccgcgt
    19201 cctgagtgaa atcgacgccg aagtagcttc cccaggtcat tcccatcgca ttgatgaaat
    19261 agttccaggt caggaaactc accatcaccg ccagacaagc acgtccctgc gcttgcttag
    19321 caaggccaat gggtaaaccg acagcaaaaa tcagcggcat attacggaat accgtccaac
    19381 cgccctcttc aataatgtgt acgatttgcg cgaataaact gttcggatcg gtcagtgatt
    19441 ccccgacaaa catcgggttt tgcagcaaga tggcaagacc caccacaatc ccggcgaagg
    19501 gaaacagcag cactggcgtg aacatcgcgc cgccaaagcg ttgaatttga ctgagcattt
    19561 ccacatcctc ttttctcaat tctgtaagta ggaagcctga ttggttatgc ggcagacctg
    19621 gagcgaggat aggaagatgc gatcggcaga acatgacgac ccaatgggat tcgtgatcgc
    19681 tttcatgctt ttcgttgggc ggtagagatc tacgttttcg cgaaaaagta gacatctgtg
    19741 agcacaggta cacagcatcg aacacatctt taaaaaaaag atttttttca atcgattaag
    19801 cagaacttgt gggcgcatta cccgtgcttg caggcaaaaa agagagctag agatgatcta
    19861 caaaagcatt gcggagcggt taagaattcg acttaattcc gcggatttca cgctaaacag
    19921 ccttcttccc ggtgaaaaaa agctggcgga agagtttgcg gtatcgcgga tgactatccg
    19981 taaagccatt gacctgctgg tagcgtgggg gctggtggtc cgccgccacg gcagcggcac
    20041 ttacctggtg cgcaaagatg tgctgcatca aaccgccagc ctgaccggac tggtggaggt
    20101 gttaaaacgg cagggaaaaa cggtcaccag ccaggtgctg atttttgaaa tcatgcctgc
    20161 gcctccggcc attgccagcc agttacggat tcaaatcaac gagcagatct acttctcccg
    20221 tcgcgttcgt tttgtggaag ggaaaccgct aatgctggaa gacagctata tgccggtaaa
    20281 actgttccgt aacctttcgc tgcaacatct ggaagggtcg aagtttgaat atattgaaca
    20341 agagtgcggg attttgattg gcggtaatta tgaaagcctg atgccggtgc tcgccgatag
    20401 actgctggcg cggcaaatga aggtagcgga acacacgcca ctgctgcgga tcacctcgtt
    20461 gtcatatagc gagagcgggg agtttttgaa ttattcagtg atgttcagaa atgccagcga
    20521 atactaggtg gagtaccatt tacggcgact ccacccggaa aaaagttaac cgatactcca
    20581 gaagagcacc gccagtaatt ggggggtgat aatgcgcagg aacatcacta acggatagac
    20641 agtggcgtaa gagagcgccg ccgcaccact ggttggatga agattattag caaacgccag
    20701 cgccggagga tcggtcatgg aacctgccag catcccgcac atggtcaggt aattcatttt
    20761 ggctaacatc cgcgccagaa tgccaacagt aatcagcgga acggcggtga tcagggcacc
    20821 ataaccaatc cagcttagcc cttcgccatt gaccagggta ttcacaaaat ccccaccaga
    20881 tttcagacca acgaccgaga ggaacagcac gatccccagc tcccgcagcg cgaggttggc
    20941 acttggcggc ataaaccagt acagcttgcc gatactgccg atacgcccga ggatcaacgc
    21001 cataatcagc ggaccgcccg ccagccccag tttcaacgcg gccgggaatc ctggcacaaa
    21061 gacgggaata gaacctaaca atacgccaag cccgatgcca ataaacactg gcagcatctg
    21121 aacctgttgc agtttttgct gcgcattccc cagcacattg gcaacggcat caattgcgga
    21181 cggacgcccc accaggttga ggatatcgcc gaactgcagg ctgatatcgc cactggcgac
    21241 cagttcgacc ccggcacggt tcaggcgcga gataacaacg tcatagcgtt ctttaaagtg
    21301 caggtcgcga atacgttttc cgagcacgtt ttcattggtg accaccacac gctcgacgcg
    21361 caaatcagtg cctttcgtag acagcgaggt atcgacctcc tgaccaatca ccagttgcgc
    21421 attatgtaaa tccgctggct gccccaccag atgcagcaaa tcgcccagtt ggataatggt
    21481 atctggcgaa ggtactttta gggtttcttc tcgtttcaga cgcgagcaga taattttgtc
    21541 gccgttgaga atcggtacat ctttaatggc taaatcatgc aggttagggt tctcaacgcg
    21601 aatattgata gtcttgatca gcgcgccgcc attggtgcgt gaagactcgt gctgctgagc
    21661 ttctgtctcg acattgacgc ggaaaataac ccgcaacatc cacatggtga acaaaatccc
    21721 gcaaatgccg aatggatacg ccatcgcgta actcatcccc atctgatcga ccatttccat
    21781 tggtgtaccc aggtcgcgca agatctgctg ccctgccccc agcgctggcg tattggttac
    21841 cgcaccggag aaaatcccca gcactaccgg cagtggaata tcaaacagtt tatgcaggat
    21901 ggcggtaacc agaccaccga tgatgacgat cagaacagca aacaggttga ggcgtaatcc
    21961 ggagacgcgc aatgaggcaa agaagcccgg ccctacctga atcccgatgg tataaacgaa
    22021 caggatcagg ccaaattcct gaataacatg cagcatatcg ctacttaatg tcatccccgc
    22081 ctgagaaaca aaatggccga cgatgatccc accaaacagc acgccgccaa tacctaatcc
    22141 tatgccgcga aatttgacgt tgccgataaa caaaccgacg actgccacca aagccagaat
    22201 actgaccgtt aatgctatat cactcatcgt ccatttctct tgagataaca ttttaattgc
    22261 tacggattat ggctgaatcg gtgaagcaga tttgccttat agcgcacatt atcacgggaa
    22321 agaacgtgcc gaaatatctt aaacagtcga aatttaacgc attgttgaag aagaaagaca
    22381 aaaaaagccc caccagaatg gcggggcaaa gagaatagct agttagctat ttaacgcggg
    22441 acgttcgctg atagcgatac gctgcgctgc gatgggttca ggctcattac gaattaaatc
    22501 aatatgcagt aaaccgttta cgaaggttgc gccagagact tccatatttt cagccagcgt
    22561 aaaactcagg ctaaatggct gattcataag cccttgatgc agccattttt tctcttcttt
    22621 tggctgctcc ggcgtgcctt ttacgctcag gcgcgtgcct tccagttgaa tctctaaatc
    22681 ttcctgacgg aaacctgcca gcgcaagggt aatgcggtag tggttatcgt cgcttttctc
    22741 aatgttgtac ggcgggaagc tctggctttc accggcgttt tgcagtgcgt tggccagttt
    22801 gtcaaaaccg atccattgac gcatcagtgg ggataaatcg aagttacgca tagtcatttc
    22861 tccttctaag aagcgagtaa gtacctgcaa atccgaagat tcgcatatgc tccctgacgg
    22921 cgagcatgga gatgtcaggc cgcgccaggc ggccttaggg aattagttga tttcgatacg
    22981 gcgcggtttt ttcgcttccg gaatcacgcg ttcgagatcg atatacagca aaccatttac
    23041 caggttagcc ccacgaacat gaatgttctc agctaactgg aatttgcgtt caaagttgcg
    23101 ttcagcgatg ccctggtaca gataggtgcg ctctttttgt tcgtcggcgt gagcaccttt
    23161 caccaccagc agattatcct gggcggtaat ttccagttcg ctctcagcaa aaccagccac
    23221 agcgatagca atgcggtaat ggttttcgtc taccagttca acgttatacg gagggtagcc
    23281 gccattactc tggctctggt tgttttctaa gtggttaaac aaacggtcaa atccaatagc
    23341 agaacggtaa agcggggata aatcaaagtt acgcataatc aatagctcct gaaatcagcg
    23401 agaatgtaag accttccaca atggacaggt caggtagcca gaacacccat caggcgcgtc
    23461 ctcatcgact acgatgtaaa atgggtctgg aaatgaactt ttcaagcctt atcagcggac
    23521 ttttttttgc agtttatggt ctattgcata gactgagggg gcagcacgaa acgttaaagt
    23581 gcgatagccg ccacagtgcg acgtaatgat gatgttattt tcagcaaggg atcgctataa
    23641 ctcatcaggc caacacccac gatcaacaga tgaatttatt atgataagaa atgtgttgtt
    23701 agcgttcatg atatgcagcg gaatgacatt actcggagga tgctccagcg tgatgtccca
    23761 taccgggggg aaagaaggaa cgtatcccgg cacccgcgcc agcgccacca tgattggcga
    23821 cgatgaaacc aactggggca ctaagtcgct ggcgattctc gatatgccgt ttaccgctgt
    23881 aatggacacg cttttactgc cgtgggatgt gttccgcaaa gacagctcgg tgcgctcgcg
    23941 cgttgaaaaa agcgaagcca acgcgcaagc caccaatgcg gttattccac cggccagaat
    24001 gcctgataac tgatttagcg atcagtttcg gtgatccaca gctggccacc ttccatccac
    24061 gccagccatt gaccatccgg cgagaagacc acggcgtccg cagaaggtgg attggcgtgg
    24121 ttttcggtta aatactcaac ctcgccgctt tgcgcatggg cacaaacaat tcgattatcc
    24181 agcacaaagc ccaaccattc tcctgacgga tgccagttaa atgcagactg aatatccgtt
    24241 ttgttatggg ttaacttgcg cggttcgccg ccctgtggcg agataagcca cagttgcaca
    24301 atgccgttat catcacgcat taaaaacgcg atttgcgtac cctgcggatt acagcgcacc
    24361 cagtggcgcg ggacgttgac taaccccgga taagcccgat gatgggtaaa ggttaaacgt
    24421 cgctgcacga cgccacgcgg tggcgcgggt agggttgttt ccgttccact taacggcgca
    24481 tcacctgccg atttccagct agcttcatct tgcggtaact caacgataaa tagctccggc
    24541 actttctcgc cctttggcga aagtgtgtcg ccaataaacg ccagcgcgtg atttcctacc
    24601 catccttctt cataagcacg attgatttca tcactgccag gctgcggcgt gggcgtggtt
    24661 ttactcacca gcacacacca gtggctaccg ctgtattcac gcggatgttg cttttgtacg
    24721 ttgaccgggc caaacggtgc agcaaccccg acgtttcgca aatccagcgc cggatcgagt
    24781 tgatgcatta catggtcgtt ataggtaaag ctcccccttt caccgttcgg gctaaagaca
    24841 tgcacatggc tgccaccgcg cagcgctcct ggcgtatacg gcgcggtaat atccattgca
    24901 tcgagattgc tcaccttgcc gccttcagca atgactccgc gtcgatgatg gaagtcgtaa
    24961 tgccatgttt catcaggatt ttcagggccg tgaataaaga catatttctc tgacttcgga
    25021 tgaacggtca ccacgccgac gtgtgcgccc tgtgacgcgc gatagataac ctcgacctcg
    25081 cctgtatgga tattcacacg ctcaatggtc tcgccggtaa acgacgcccc agaaggacgc
    25141 acgtcaaata ccagccactg gctgtcgggc gtccaggtat tggtatttgt attggtattg
    25201 gtattggtga gcaggtgatt acggggagca aaggttatct gtttcatggc gcggtcctga
    25261 tacaaaacgg ttcccgccat catacttcac aaggacttca ctttcagaca atagtctttt
    25321 gccatcgtga tgaatgtgag tgaaaagatg gaacatttcg acgtggcgat tattggcctc
    25381 ggcccggcag ggtcggcgtt ggcacgaaag ttagccggca aaatgcaggt gatcgcgctg
    25441 gataaaaagc accagtgcgg tactgaaggt ttcagcaaac cttgcggcgg tctgctggca
    25501 ccggacgcgc agcgatcttt tattcgcgat ggactgacgc ttcctgtcga tgtgatcgcc
    25561 aatccacaga ttttcagcgt caaaactgtc gacgtcgccg catcgctcac gcgtaactac
    25621 cagcgaagct atatcaatat taatcgccat gctttcgact tgtggatgaa atcgctgatc
    25681 cccgccagcg ttgaggttta tcacgatagc ctgtgccgaa aaatctggcg tgaggatgat
    25741 aaatggcatg tcatttttcg tgcagacggc tgggagcaac atattaccgc ccgctatctg
    25801 gtcggtgccg atggcgcaaa ctcgatggtg cggcgacatc tctacccgga tcatcaaatc
    25861 cgtaaatatg tcgctatcca gcagtggttc gcagagaaac atccggtgcc gttctactcc
    25921 tgcatctttg ataatgcgat aactgactgt tactcatgga gtatcagcaa agatggttat
    25981 tttatctttg gcggtgccta tccaatgaaa gacggtcaga cgcgtttcac gacgctgaaa
    26041 gagaaaatga gcgcctttca gttccagttt ggcaaagcgg tgaaaagtga gaaatgcacg
    26101 gtgttgtttc cctcacgctg gcaggatttt gtctgtggta aggacaacgc ctttctgatt
    26161 ggtgaagcgg cgggatttat cagcgccagc tcgctggagg ggattagcta tgcgctggat
    26221 agcgcagaga ttctgcgtgc ggtgttcctg aaacagccgg agaagagcaa cgccgcttac
    26281 tggcgcgcca cacacaaact gcgtttaaaa ctcttcggca agatagtaaa aagccgatgc
    26341 ctgaccgcac cggctttaag aaagtggatt atgcgcagtg gtgtggcgca tattccacag
    26401 ttgaaagatt atccaacgcg cttcacatca cccaccagca ggatgtaaga gagcgcgcca
    26461 atcaacgcga cggcggagat ataaaccagt gcaggcgcga aaccgtaacc ctgcgccagg
    26521 taccccacca ccagcggaac ggtgatgccg cccagaccac cggcgaagtt aaacacgccg
    26581 ccggttaaac caatcaggcg catcggtgcc agagaagaga ccagcgacca ggtaatcgaa
    26641 gcaaaaccgt tgccgaagaa tgccagcgcc atcaggcaca taatcatcat cggatcgttg
    26701 gtgtagttag cgcccataat gcaggtggag atcagcaagc cgcagataat cggcgtttta
    26761 cgcgcaaagc ccagtgaaaa gcccttacgt accagcagat ccgcgaccca gccagagagc
    26821 aggacgccga caaacgccgc gaggaatggc accgtggtca taaagcctgc tttcagcgcc
    26881 gtgattcctt tttcctgggt taaatagttc gggaaccagg ttaagaaaaa ccacagtgca
    26941 gaagccaccg caaattgccc aagataaacg ccgatcagtt tacgatggaa caccagtttc
    27001 cagtctttgg ctgttaacgg ctgacgcgcc tctttcttca ccggcgcatc accatccacc
    27061 agaccgccgc catcacgaat gtaatccagt tcagctttgc tgataccttt ggtcaggcgc
    27121 ggcggctgat aaaccttaaa ccaaatcagc gaccagataa tgccgatacc accagtgaca
    27181 atgaacaccc agtgccagct caacatctcc tgaatccaga tcagcagcgg agtcaaaaac
    27241 gccagaccga caaactgacc agacgtataa aaaccaacgg cggaagcgcg ttcatgttcc
    27301 gggaaccagc tggtcaccat ccggttattg gtcgggaaag cgggcgcttc gaaaataccg
    27361 gttatcgcac gcagaccaat taatgacatt aatcccgtgg caaagccttg gaataaagtc
    27421 gccactgacc aaccaaatat cgcaataaaa taagtcacgc gagaacctac gcgatctaaa
    27481 aaccaaccgc cggggatctg gcatagcgta taaagccagg cgaaggccga aaatacatag
    27541 cccatttccg ctttcgtaat gccgaactct tcctgaatat gggcggaagc cacggccagg
    27601 ttagcgcggt caacataaca aatgaccacc gtaataaaga tcatcaccag cgtcagataa
    27661 cgccgacgcc ccggctttgc tgcattaacg ggaatatcca tagcgagctt tctccagatt
    27721 ttgggcatag cgaagccgct caccatgccc tgtaatttac agagggttat atttttgtat
    27781 tgctgtttta gtgcccgatg aggggcttac gtggcaggaa ttaccactct gctacgctgt
    27841 tatcttcatg acgccagagc ggattacgcc agtccggggc atttttactg aactcaataa
    27901 ctttagcttc gtcgatttcc acacctaagc ccggtttcgt taacggttta aagaagccgc
    27961 caaccatgct gaagtcttct ttgtttttca caaagtcgag taactccgcg cctttgttgt
    28021 aatgaatgcc catactttgt tactgaagta ccgcgttata ggaaacgaag tcgatatgca
    28081 ggcaagccgc cagtgcaatc ggtccgaacg gacagtgcgg cgcaagggtc acatcatagg
    28141 cttctgccat tccagcaatt ttgtagcatt cggtaatacc gcctgcatgg gagagatccg
    28201 gttgcagaat cgaaataccg cctgcctcca gcacgcgttt aaaatcgaaa cgtgagaaca
    28261 tgcgctcacc cgccgccagt ggaatatgcg tttgtgccgc caatttcggg tagtattcgg
    28321 cttgttccgc cagcaccggc tcttcaataa acagcgggcg atacggctcc agttctttaa
    28381 tcagcacttt cgccattggc gcgctgacgc gaccgtggaa atcaagacca aactcaatct
    28441 gattgccaaa agcttcacga atttgtgcca cggtgttgac tgccgcatct accgcgcggg
    28501 agttatcaat tagccccagt tcttcacaac cgttcagttt gaaggtatcg aagccgattt
    28561 cgcgcagggt tttaatgccg tcgataacat ccgccggacg atcgccgccg acccaactgt
    28621 aggctttaat tttgtcgcga accaggccgc ccatcagttg ccagactggc gcattcagca
    28681 ctttgccttt gatatcccat aacgcctggt cgatcccggc gatggcgctc atcaggattg
    28741 gaccgccacg ataaaatccg gcgcgataca tcacttgcca taagtcgttg atgcgcgaag
    28801 gatcctgacc aatcaaatag tcacccagct cgtgaactgc cgcttccacc gtacgggcgc
    28861 ggccttcaat cacgggctcg ccccaaccga ccacgccttc atcggtttca attttcagga
    28921 acatccagcg gggaggtaaa cgatacgtgg taattttggt gattttcatt gcactgcctc
    28981 tcgatacgcc ttaacaaatg ctgctgcctg ctgcgcggtg cgttctacgg actgcccggc
    29041 gcgataaaga tcgctgccta agcccgcccc aacacaacct gcgtctatcc actgcgccag
    29101 gttttctggc gtcacgccgc caacggcaaa gactgcgatg tccggtggta ataccgcttt
    29161 taacgctttg atgtattgcg gaccaaaagc cgatgacgga aatattttca gcgcctgcgc
    29221 gcccgcttcg agcgcggtaa aggcttcggt cgccgtcgcg cagccggggc aaacggtcat
    29281 gccgtagccc accgcgcggc ggatcacttc actatggata ttgggcgtaa cgatgagctg
    29341 acagcccatc ctggcgagcg catcgacctg ttcaggtttc agtaccgtac ctgcgccaat
    29401 caacgccttg tcgccatacg catcaacgat ggcgggaatg ctctgctccc attgtgggga
    29461 attcagcggg atttcaaccg cgtcgaaccc ggcgtcaatc accgcgccaa catgcgccag
    29521 cgcctcgtcg ggcgtaatgc cgcgcaaaat ggcgatcagc gggagtttag tttgccactg
    29581 catgagcgat gctccttata ccagcctgaa atgccgtgtc gcccgccacc gccgccacgt
    29641 cgcaacccat cgcctgaaag gcttgctggt agcgcgcggt cagcgatgtt ccggcgacaa
    29701 gggtgatggc gtgttgatgg gtcacatagt cgcgcatact ggcaacctct gcgccaatca
    29761 acaaaccaga gagaaattcg ctgacctgtt cgcggggaag tgttcccagc acatgcgagg
    29821 cgcgaacttc aaaaagctgc ggcaatatgg cgggcgcatt aaggccgcgc tcaaggccag
    29881 ccgcgaaggc atcggcagag ttttcctgcg gcggcaaacc tgcgccaatc aatgagtgat
    29941 ttaacagtaa atgatgtaat tcaccggtca tcacggtgcg aaaatcgttg atttgctggc
    30001 tatcggcctg cacccatttg caatgcgtac cgggcataac ataaagagag gaaggagcca
    30061 gagctcgcgc gccgatcaat tgtgtttctt cgccgcgcat cacattgtgg ttatcgtcat
    30121 gagagacgca taatccggga ataatccaga tattgtcgcc aacagacgtt aattgttcgc
    30181 caatagacga aaaacgggca ggaacagata aatacggagc aactttccag ccgacattgc
    30241 tgccgaccat tcctgccatc accactggcg tattttcttc acgccagtcg gtcgtgactt
    30301 ctgctaacac tgcagccgga gattttccgt tcaggcgcgt gacgcctgct tctgattgcc
    30361 tgctctccag gcagtggtcg ccctgataaa gccaggcgcg cagattggtc gatccccagt
    30421 caattgcgat gtagcgagct gtcatgtgat ttcctttaac cttcgtgtcg agctggcaat
    30481 catggtaagc gccgcctgct ctgccgcatc gccgtcctga tgccgtatcg catcgaacag
    30541 cgccttatgt tcctggagcg tttgcggcat gttggcctca tcgcccatcc aggttcgttc
    30601 aaaaaccgcc cgctgcagcg aactgatcgc aatgctaagt tgctgtaaca ccgggttatg
    30661 caccgactgc agcaccgcct cgtggtagcg aatatccgct tcgttaaacg cttcgcggtc
    30721 ctgattgttg gcaatcatct cgttcagcgc cgattcaatc tgcgccagat cgctggaggt
    30781 cgcgcgctct gctgcccaac gggcaatcgc cggttccacc agatttcgca cttcgctcat
    30841 ggcactgata agccgtgggt cgtagtcgtt ttccagcacc cattgcagta cgtcagtgtc
    30901 gaggtaattc cactggttac gcggtgccac aaacgccccg cgataacgtt tcatttcaat
    30961 cagccgcttc gccatcagcg aacggaacac ctcacggatg atgttgcgcg aggttgcaaa
    31021 ctcttcacag agctccgcct cagccgcaag tggcgagcca ggtacgtatt tgccgtgaac
    31081 gatctgttta cccagcgtaa tgacaatgcg atcggtttta ttgagagtca tggagagtcc
    31141 ttgtgctctg tatgttcttc tctactttac cccgatcgat gcataacgcg gcaactttgt
    31201 agtaccagcg tgatgacgtt cgcgtttgcc gcgcgcgtaa tgtagtacaa acttatattg
    31261 ttgcgctaca atttagatca aaaaaagaac aacacataaa aaaatgacat gcgtcgggca
    31321 gaaatctgaa aagggatatc aggcgctaaa caggagggaa agaagagtat gctttcaacg
    31381 gcttagctac tcgtttaaag gattaatcat gaagttgaat tttaagggat tttttaaggc
    31441 tgccggttta ttcccactgg cgctgatgct ttcaggctgt atctcgtatg ctctggtttc
    31501 ccataccgca aagggtagtt caggaaagta tcaatcgcag tcagacacca tcactgggct
    31561 atcgcaggca aaagatagta atggaacaaa aggctatgtt tttgtagggg aatcgctgga
    31621 ttaccttatc actgatggtg ccgatgacat cgttaagatg ctcaatgatc cagcacttaa
    31681 ccggcacaat attcaggttg ccgatgacgc aagatttgtt ttaaatgcgg ggaaaaagaa
    31741 atttaccggc acaatatcgc tttactacta ctggaataac gaagaagaaa aggcactggc
    31801 aacgcattat ggttttgcct gtggtgttca acactgtacc aggtcactgg aaaacctaaa
    31861 aggcacaatc catgagaaaa ataaaaacat ggattactca aaggtgatgg cgttctacca
    31921 tccatttaaa gtgcgatttt atgaatacta ttcacccaga ggcattccgg atggtgtttc
    31981 cgcagcatta ctgccagtga ctgttacact ggacatcatt actgcaccgc tgcaatttct
    32041 ggttgtatat gcagtaaacc aataatcagt aagcgggcaa acgcgtttat gctgtttgcc
    32101 cgcccacaga ttaattcagc acatacttct caatagcaaa cgccacgcca tcttcaaggt
    32161 tggattttgt gacaaagttc gccacttctt tcaccgaagg aatagcgtta tccatcgcca
    32221 caccgacgcc tgcatattca atcattgcga tgtcgttttc ctgatcgcca atcgccatga
    32281 tttcttccgg tttaatacct aacacgtcgg ccagtgattt cacacccgta cctttgttaa
    32341 cgcgtttatc gaggatttcg aggaagtacg gcgcactttt cagcacggta tatttctctt
    32401 tcacttcctg cggaatacgc gcgatagcct ggtcgaggat ggcgggttca tcaatcatca
    32461 tcactttcag gaactgggta ttggggtcca ttttctccgc ttcgcagaac accagcggaa
    32521 ttgtggcaac gaaggattca tgcaccgtgt agtagctgat atcgcggtta gcggtgtaca
    32581 gcgtggtgcg gtccagggcg tggaaatgag aaccgacttc gcgagagagt ttttccagga
    32641 aacgatagtc gtcatagctg agagcggttt gcgccacggt gctaccatca gcggccttct
    32701 gtaccagcgc gccgttataa gtaatgcagt agtcgcccgg ctgttccata tgcagctctt
    32761 tcaggtagtt gtgcacacct gcatacggac gacccgtcgt tagcacgaca ttcacgccac
    32821 gggcgcgagc tgcggcaatc gcatttttaa cggcgggtga aatggtgtga tcgggcagca
    32881 gaagggtgcc atccatatcg atagcaatga gtttgatagc catgagttcc caaggtagat
    32941 tagttcctga cccatgctaa cgcgattccg ctcaaaaatc agtacaacac ccgagggaaa
    33001 aggggatgca acgcgggttg catgctcccc atttgcttag cggaagagtt tccctttcag
    33061 cagttccatg cctgcggaaa gcagatcgtt attggcttgt gctgacactt caccttgcgg
    33121 tgagagcgca tcaataatct tcggcaattg ctctgccagt aaactggaag ccgtactggt
    33181 atccacgcca agtttttgcc cgagatcgga caccgcattt gtgccgagcg ccgactccag
    33241 ttgctcgccg ctaactgatt gatttctctg ttgattactc aaccaggttg agagaatggc
    33301 ccctaagccg ccactttgca gtttttccag cagcacctga atgccaccct gctcctcaac
    33361 ccaacttaaa atcgcctgat atttccccgc atcgcctttc agaaaggcac cgacaacttc
    33421 atcaaaaagc cccatgataa tcacctgtaa agcgttacgt gttgacccaa aaagtataga
    33481 tctgcgggat gataattgcg gattgcagaa ataaaaaggg cggagatgat ctccgccctt
    33541 ttcttatagc ttcttgccgg atgcggcgtg aacgccttac ccggcctaca aaatcatgca
    33601 aaatcaatat attgcaggag tctcgtagga ctaataagcg tagcgcatca ggcacgctcg
    33661 catggttaac gccattaaat atcaatattc gccgctttca gggcgttctc ttcaataaac
    33721 gcacgacgcg gttctacggc gtcgcccatc agcgtggtga acaactggtc agcggcaatc
    33781 gcatccttaa cggtaacgcg cagcatacga cggctttccg ggtccatggt ggtttcccac
    33841 agctgttccg ggttcatctc gcccagacct ttataacgct ggatggagag gccacgacgg
    33901 gactctttca ccagccagtc cagcgcctgc tcgaagctgg ctaccggctg acgacgctcg
    33961 ccacgttcga taaacgcatc ttcttccagc aagccacgca gtttctcacc cagcgtgcag
    34021 atacgacgat attcgccacc ggtgataaac tcgtgatcca gcggatagtc agtatccaca
    34081 ccgtgggtac gcacgcgaac aatcggctcg aacaggtttt gttcggcatt ggtgtggaca
    34141 tcaaacttcc actggctgcc gtgctgttct ttgtcgttca gttcgctgac cagcgcgttc
    34201 acccagcggg taacggtctg ctcatcagaa aggtcagctt ccgtcaacgt cggctgatag
    34261 ataagctctt tcagcattgc tttcggataa cgacgctcca tgcggttgat cattttctgc
    34321 gtcgcgttgt actcagatac cagtttctct aacgcttcgc cagccaatgc cggtgcactg
    34381 gcgttggtgt gcagcgttgc gccgtccagc gcgatagaga tctggtactg atccatcgct
    34441 tcgtcgtctt taatgtactg ttcctgcttg cctttcttca ctttgtacag cggcggctga
    34501 gcgatgtaga cgtgaccgcg ttcaacgatt tccggcatct gacgatagaa gaaggtcaac
    34561 agcagcgtac gaatgtgcga gccgtcgacg tccgcatcgg tcatgatgat gatgctgtga
    34621 taacgcagtt tgtccgggtt gtactcgtca cgaccgatac cacagccaag cgcggtgata
    34681 agcgtcgcca cttcctgaga agagagcatc ttatcgaagc gcgctttctc gacgttaagg
    34741 attttaccct tcagcggcag aatcgcctgg ttcttgcggt tacgcccctg cttcgcagag
    34801 ccgcccgcgg agtccccttc caccaggtac agttcggaaa gcgccggatc gcgttcctgg
    34861 cagtctgcca gtttgcccgg caggccagct aagtcgagcg cacctttacg gcgggtcatt
    34921 tcacgcgcgc gacgcgccgc ttcacgggca cgggcagcat cgataatttt gccaaccacg
    34981 attttcgcat cagttgggtt ttccagcagg tattccgcca gcagttcgtt catctgctgt
    35041 tcaaccgccg atttcacctc agaagaaacc agtttgtctt tggtctggga ggagaatttc
    35101 gggtccggca cttttacgga aacgaccgca atcaggcctt cacgcgcatc gtcaccggtg
    35161 gcgctgactt tggctttttt gctgtagcct tctttgtcca tgtaggcatt cagggtacgg
    35221 gtcatcgccg cacggaagcc tgccaggtga gtaccgccgt cacgctgcgg aatgttgttg
    35281 gtaaagcagt agatgttttc ctggaagcca tcgttccact gcagcgccac ttcaacgcca
    35341 ataccgtctt tttcggtgga gaagtagaag atatttgggt ggatcggcgt tttgttcttg
    35401 ttcagatatt caacgaacgc tttgatgccg ccttcatagt ggaagtggtc ttctttgccg
    35461 tcacgcttgt cgcgcagacg aatggaaacg ccggagttga ggaacgacaa ctcacgcaga
    35521 cgtttcgcca gaatttcata ttcgaactcg gtcacattgg tgaaggtttc gaggctgggc
    35581 cagaaacgca ccatggtgcc ggttttttca gtctcgccgg taaccgccag cggagcctgc
    35641 ggtacaccgt gttcgtagat ctgacggtga attttaccct cgcgctgaat aaccagctcc
    35701 agtttttgcg acagggcgtt tactaccgaa acaccaacgc cgtgcagacc gccggacact
    35761 ttataggagt tatcgtcaaa tttaccgcct gcgtgcagaa cggtcatgat cacttccgcc
    35821 gccgatacgc cctcttccgg gtgaataccg gtcggaatgc cgcgcccgtc atcctgtaca
    35881 gagacagagt tatcggcgtg aatggtgacg ataatttctt tacagtgacc cgcgagcgct
    35941 tcgtcgatag cgttatctac cacctcgaat accatgtggt gcagaccggt gccgtcatcc
    36001 gtgtcgccga tatacatacc cgggcgctta cgcaccgcat ccagcccttt caggactttg
    36061 atactggagg agtcataaga attcgacatc aacgtttctc gctcatttat acttgggtta
    36121 atccgttatt ttaccctttt ccacggtaaa catcttcgaa ttttcgtccg acatgtctat
    36181 aacgtgttca gcactgatcg cgctgacaaa gacctgtgat tgcgtcgctt ttaagcggct
    36241 ggcaagcaac ccgcgacgct catcatcaag ctcagaggca aaatcatcta tcaggtagag
    36301 acaccgccgc ccgctttcac gagtgaggaa ctctccttgc gccagacgta aggcgcacat
    36361 caacagctta agctgcccac gcgataaggt atcttccacc ggcgcaccgt cggcgcgaat
    36421 gcgtaagtcc gctttatgcg ggccatgcgc ggtgtaggtt agctggcgat cgcgttcaaa
    36481 attacgttcc agcacctcag cgtactctgt ctctttctcc cagccgcgct ggaaagagaa
    36541 agtcagagaa aactcaggga gaaattgctt acaggtatca tccatatcgg ccgcgatacc
    36601 ggcgctatac tccgcgcgcc aggtgctgat ttgctccgcc agcgggatca gttctttatc
    36661 ccacgggcgt agctgttcgt aacgagtcac ctggcgcagc gccgcattgc gctgcttgag
    36721 caatcgcttg agattgctcc aggcggtgaa aaatccaggt tcgttgtgaa agcatcccca
    36781 gtcgaggaat gctcttctgt atttggggcc gccgttgagt aaagtaaacc cttctggcgt
    36841 tatcaactgc atcggcatca ggtgcgccag ttccgcgact ttatgcccgt ccgtaccgtc
    36901 aatacggact ttgctgtcgc cctgtttgtc tttggttaag ccaatcgccg tctcgcgctc
    36961 ttcgccctgt aatcgcccgt ggagaacaaa tgcctcctgc tcatggcgaa tgacgcgacc
    37021 aatctgcaaa ctgcgaaacg cccgaccatg gccgagcgta tagatggctt ccagcacgct
    37081 ggttttgcca ctgccgttgg cacctaccag aaagttaaag ccgggagata aagcgagatc
    37141 cgcggtttca atattgcgga aatcgcggat caacaagcgg gtgagggaca ttacagtctc
    37201 attggcatga caacataggc agccgactgt gatgcggcat cttcaatctg cacgctggaa
    37261 accgaatcgg tcagcatcat gcggacgttt tcgcatttca gcgcgttcag aacatccagc
    37321 acatagctga cgttgaagcc gatttccatc tccgcaccgc tataggtaac gtcgaggatc
    37381 tcttccgctt cttcctgttc cgggttgttg gcagtaattt tcagctggtt ttcgctgaca
    37441 tacaggcgca caccgcggaa tttctcgtta gagagaatcg ccgcgcgagc aaacgcctgc
    37501 ttgagcagat cgcagcctgc ttccagatgt ttgtccgggt tcttcggcag aacgcgacgg
    37561 taatccggga agcgaccatc caccagtttc gaggtgaaaa taaagtcgcc aacgtgggca
    37621 cgaatgttat tgctgccaat ctgcacacgc agcagattgt cgccgccgtc gagcatacgc
    37681 atcagttcaa tcacgccttt acgcggtacg atcaccgaat ggcttggcaa agattgacca
    37741 attggcattg aacagaccgc cagacggtgg ccgtcggttg ccacggtgcg cagttcttca
    37801 ccttcggttt caaacagcat accgtttaag taatagcgaa catcctgatg cgccatcgaa
    37861 aactgggttg cttcaatcag acgcttcatc gtcgcctgcg gcagggtaaa ttcgacttcg
    37921 ctctgccagt catcgaggtt cgggaaatcc gccgctggca gggtagacag cgaaaaacgg
    37981 ctacgcccgg agcgcaccag catccgttca ccttccagct gcacggcaat ttccgcgcct
    38041 tcaggcagac cacggcagat atcaaagaat ttgcgcgccg gaacggtcgt cgctcctggc
    38101 tcgtgtggct gaaccagcgc aacgcgtgcc accatttcca tctcgagatc agtaccggtc
    38161 agcgacaacg taccgtcagc aacctgtaac agcagattac cgagaatcgg tagcgtagga
    38221 cgaccaccta acggaccgct cacctgttgt agcggtttta ataaatgctc acgttctacg
    38281 gtaaatttca taggtttacg atgacaatgt tctgattaaa tttgaaaaat cttctttgat
    38341 atcgtggctc tcttcacgca actgctcgat cttacggcag gcatgaagca ccgtcgtgtg
    38401 gtcacgccca ccaaacgcat cgccaatctc tggcagactg tggttagtca gctctttcgc
    38461 tagcgccatc gccatctggc gcggacgcgc caccgagcgg gatcgacgct tggaaaggag
    38521 atccgcgact ttgatcttgt agtactccgc caccgtcttc tgaatattgt cgatggtgac
    38581 cagtttttcc tgcaatgcca gcaagtcgcg cagcgcctca cgcacgaagt cgatggtgat
    38641 cgcccgtccg gtaaagttgg cgttagcaat aacgcggttc agcgcccctt ccagctcacg
    38701 tacgttagat cgtagacgct tggcgataaa gaacgccact tcacccggca aacgaatgtc
    38761 attttcgtcg gcctttttca tcaggatcgc cacacgggtt tccagctctg gcggttcgat
    38821 cgccacagtc agtccccaac cgaagcggga tttcaaacga tcctcaacgc cgttgatctc
    38881 tttcggatag cgatccgagg tgagaatgat ctgttgatta ccttccagca gggcgttgaa
    38941 ggtgtggaaa aactcttcct gagatcgttc tttattagca aaaaactgaa tatcgtcgat
    39001 cagcagtgca tctacggaac ggtagtagcg tttaaactct tcgatcgcgt tgttttgcag
    39061 ggctttaacc atgtcctgaa caaagcgctc ggagtgcata taaaccactt tggcattcgg
    39121 cttgcgcgcc ataatgccgt tacccaccgc atgcagcagg tgagttttac ccagacccgt
    39181 gccgccataa aggaacaacg ggttataggc accgccaggg ttatccgcca cctggcaagc
    39241 cgccgcgcgc gccagttggt tagatttacc ttcaacgaag ttatcaaacg tgtgtttgac
    39301 gtttacgtta gaacgatagg tcggttctgc cggggccggg acgttatccc aacctgagcg
    39361 cgtagaaggc gcagcacgtt gcggctgcgt ttgcgccacc tgtgcagggg ccgcgacgtt
    39421 gctcgtcact gccgcttgtg gcgtttgcgt caccggtttg gtgccgactt caaaacgcag
    39481 ctgtggggca tccgctccgc agaaactggt tagcagtcca ttgatattat taaggtactt
    39541 gtcccgtacc caatcgagga caaaacggtt tggcgcgtac agggccagcg tgttatcgct
    39601 cagttccgcc tgcaatgggc gtatccacat actgaattct gtggctggta actcatcctg
    39661 caatcgggca agacactgct gccaaagcga aagtgacacg gcggactcca ctcgaacaaa
    39721 agtcgataat gactaaggct gaaacattca tgattgttga cgtacgtcga aaagaccctg
    39781 cttgagggtg acgcacgaac cgctgtctgc ggttatatgc ccgatcaaga tcctgcaaaa
    39841 cgatcgggac cgcggatcat agcctaaact gcgcaagaga tcttctgttt ctcacagatt
    39901 ttccctattt atccacagga ctttccagaa ctcgctaagt gtaaacgatc ctgccgcgag
    39961 gcgggcacga tttacgccgc atattggaaa atttaatgac catagacaaa aattggctta
    40021 atcgatctaa taaagatcca ggacgatcct tgcgctttac ccatcagccc gtataatcct
    40081 ccacccggcg cgccatgctg gtttccactg gtgtgaggtc gtacattttc cctgcgaaaa
    40141 ggtgcggaaa agcgcggtaa ataaggaaag agaattgact ccggtgtgta caattattac
    40201 aatccggcct ctttaatcac ccatggcttc ggtgtccatc gtttcatttt tcggcggata
    40261 tccaataaag ccattgaatt tattcaagtt taggtagaaa tcgccatgaa acgcactttt
    40321 caaccgtctg tactgaagcg caaccgttct cacggcttcc gtgctcgtat ggctactaaa
    40381 aatggtcgtc aggttctggc acgtcgtcgt gctaaaggcc gcgctcgtct gaccgtttct
    40441 aagtaataaa gctaacccct gagtggttaa gctcgcattt cccagggagt tacgcttgtt
    40501 aactcccagt caattcacat tcgtcttcca gcagccacaa cgggctggca cgccgcaaat
    40561 taccattctc ggccgcctga attcgctggg gcatccccgt atcggtctta cagtcgccaa
    40621 gaaaaacgtt cgacgcgccc atgaacgcaa tcggattaaa cgcctgacgc gtgaaagctt
    40681 ccgtctgcgc caacatgaac tcccggctat ggatttcgtg gtggtggcga aaaaaggggt
    40741 tgccgacctc gataaccgtg ctctctcgga agcgttggaa aaattatggc gccgccactg
    40801 tcgcctggct cgcgggtcct gatagccctc attcgggtct atcaacgcct gattagtccg
    40861 ctactcgggc cgcattgtcg tttcactcca acctgttcaa gctacggaat tgaggcattg
    40921 cgcaggtttg gagtgataaa aggcagttgg ttgacggtga aacgcgtatt aaaatgccac
    40981 cctttacacc ctggtggtga cgatcccgtc ccgcccggac catttgatac cagagaacac
    41041 taacgatgga ttcgcaacgc aatcttttag tcatcgcttt gctgttcgtg tctttcatga
    41101 tctggcaagc ctgggagcag gataaaaacc cgcaacctca ggcccaacag accacgcaga
    41161 caacgaccac cgcagcgggt agcgccgccg accagggcgt accggccagt ggccagggga
    41221 aactgatctc ggttaagacc gacgtgcttg atctgaccat caacacccgt ggtggtgatg
    41281 ttgagcaagc tctgctgcct gcttacccga aagagctgaa ctctacccag ccgttccagc
    41341 tgttggaaac ttcaccgcag tttatttatc aggcacagag cggtctgacc ggtcgtgatg
    41401 gcccggataa cccggctaac ggcccgcgtc cgctgtataa cgttgaaaaa gacgcttatg
    41461 tgctggctga aggtcaaaac gaactgcagg tgccgatgac gtataccgac gcggcaggca
    41521 acacgtttac caaaacgttt gtcctgaaac gtggtgatta cgctgtcaac gtcaactaca
    41581 acgtgcagaa cgctggcgag aaaccgctgg aaatctcctc gtttggtcag ttgaagcaat
    41641 ccatcactct gccaccgcat ctcgataccg gaagcagcaa cttcgcactg cacaccttcc
    41701 gtggcgcggc gtactccacg cctgacgaga agtatgagaa atacaagttc gataccattg
    41761 ccgataacga aaacctgaac atctcttcga aaggtggttg ggtggcgatg ctgcaacagt
    41821 atttcgcgac ggcgtggatc ccgcataacg acggtaccaa caacttctat accgctaatc
    41881 tgggtaacgg catcgtcgct atcggctata aatctcagcc ggtactggtt cagcctggtc
    41941 agactggcgc gatgaacagc accctgtggg ttggcccgga aatccaggac aaaatggcag
    42001 ctgttgctcc gcacctggat ctgaccgttg attacggttg gttgtggttc atctctcagc
    42061 cgctgttcaa actgctgaaa tggatccata gctttgtggg taactggggc ttctccatta
    42121 tcatcatcac ctttatcgtt cgtggcatca tgtacccgct gaccaaagcg cagtacacct
    42181 ccatggcgaa gatgcgtatg ctgcagccga agattcaggc aatgcgtgag cgtctgggcg
    42241 atgacaaaca gcgtatcagt caggaaatga tggcgctgta caaagctgag aaggttaacc
    42301 cgctgggcgg ctgcttcccg ctgctgatcc agatgccaat cttcctggcg ttgtactaca
    42361 tgctgatggg ttccgttgaa ctgcgtcagg caccgtttgc actgtggatc cacgacctgt
    42421 cggcacagga cccgtactac atcctgccga tcctgatggg cgtaacgatg ttcttcattc
    42481 agaagatgtc gccgaccact gtgaccgacc cgatgcagca gaagatcatg acctttatgc
    42541 cggtcatctt caccgtgttc ttcctgtggt tcccgtcagg tctggtactg tactatatcg
    42601 tcagcaacct ggtaaccatt attcagcagc agctgattta ccgtggtctg gaaaaacgtg
    42661 gcctgcatag ccgcgagaag aaaaaatcct gattcggtga gttttcgcta aaataagggc
    42721 ggtcagttga ccgccttttt tcttttcgta gggcggataa gcaccgcgca tccgccacac
    42781 aaagcaacag gaacatcatg agcgataatg acactatcgt agcccaggcc acgcctccgg
    42841 gacgtggcgg cgttggcatc ctgcgcatct ccggcttcaa agcccgtgaa gttgccgaaa
    42901 ccgtgctggg taaactgcct aagccgcgct acgccgatta tcttccgttt aaagacgccg
    42961 acggcagcgt gctcgatcag gggattgcgc tatggttccc tggcccgaac tcgttcaccg
    43021 gcgaagatgt gctggaactg caaggtcatg gcggtccggt gatcctcgac ctgctgttaa
    43081 aacgcattct gaccattccc ggcctgcgga ttgctcgccc tggtgagttt tccgaacgcg
    43141 cgtttcttaa cgataaactt gacttagccc aggccgaggc gattgccgat cttatcgacg
    43201 ccagttcgga acaggcggcc cgttcggcac ttaactcgct gcaaggcgca ttctccgcac
    43261 gggttaatca tctggtagaa gccctcaccc acttgcgcat ttacgtcgaa gcggcaattg
    43321 atttccctga tgaagagatc gatttcctct ccgacggcaa aattgaagcc cagcttaatg
    43381 acgtgatggc cgatctcgat gcagtgcgtg ctgaagcacg tcagggtagt ttgttgcgcg
    43441 aagggatgaa agtagtgatt gccggacgtc ctaatgccgg taaatcgagc ctgttaaacg
    43501 cgctggcggg ccgtgaagcg gcaatcgtaa ccgatatcgc cggaaccacg cgtgacgtgc
    43561 tgcgtgagca tatccacatt gacggaatgc cgctgcatat catcgacact gccgggctac
    43621 gtgaggccag tgacgaagtg gaacgtattg gtatcgagcg cgcgtggcag gaaattgaac
    43681 aggccgaccg cgtgctgttt atggtcgatg gcaccacgac agacgccgtt gatccggcag
    43741 agatctggcc ggaatttatc gcccgtctgc ccgcgaaact gccgatcacc gtggtacgca
    43801 ataaagccga tatcaccggc gaaacgctgg gaatgagtga agtgaacggt cacgcgttaa
    43861 ttcgtctctc ggcaaggact ggtgaaggcg tggacgtgct gcgtaaccat ctcaaacaga
    43921 gcatgggctt tgacaccaat atggaaggcg gcttcctggc gcgccgtcgc cacttacagg
    43981 cgctggaaca ggcagcggag catttgcagc aaggtaaagc gcaactgttg ggagcctggg
    44041 caggtgaact gctggcggaa gagttgcgcc tggcacagca gaacttaagc gaaatcaccg
    44101 gggaatttac ttcagacgac ctgctggggc ggattttctc cagcttctgt attggtaagt
    44161 aaccgcgctt acgaagccgt attctgactg tcagatgcgg cttcgcttca ttgttagcac
    44221 tcctgttatg cct
//
LOCUS       DAZSIO010000014        42799 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZSIO010000014 DAZSIO010000000
VERSION     DAZSIO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.014"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            172..654
                     /gene="sseA"
                     /locus_tag="ACHX5T_000672"
                     /pseudo
     CDS             172..654
                     /gene="sseA"
                     /locus_tag="ACHX5T_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
     gene            1316..1441
                     /gene="timP"
                     /locus_tag="ACHX5T_000673"
     CDS             1316..1441
                     /gene="timP"
                     /locus_tag="ACHX5T_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR8465995.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(1473..2249)
                     /gene="sseB"
                     /locus_tag="ACHX5T_000674"
     CDS             complement(1473..2249)
                     /gene="sseB"
                     /locus_tag="ACHX5T_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR8465996.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2391..3674)
                     /gene="pepB"
                     /locus_tag="ACHX5T_000675"
     CDS             complement(2391..3674)
                     /gene="pepB"
                     /locus_tag="ACHX5T_000675"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR8465997.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3733..3933)
                     /gene="iscX"
                     /locus_tag="ACHX5T_000676"
     CDS             complement(3733..3933)
                     /gene="iscX"
                     /locus_tag="ACHX5T_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR8465998.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3945..4280)
                     /gene="fdx"
                     /locus_tag="ACHX5T_000677"
     CDS             complement(3945..4280)
                     /gene="fdx"
                     /locus_tag="ACHX5T_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR8465999.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4282..6132)
                     /gene="hscA"
                     /locus_tag="ACHX5T_000678"
     CDS             complement(4282..6132)
                     /gene="hscA"
                     /locus_tag="ACHX5T_000678"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR8466000.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(6149..6664)
                     /gene="hscB"
                     /locus_tag="ACHX5T_000679"
     CDS             complement(6149..6664)
                     /gene="hscB"
                     /locus_tag="ACHX5T_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR8466001.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6760..7083)
                     /gene="iscA"
                     /locus_tag="ACHX5T_000680"
     CDS             complement(6760..7083)
                     /gene="iscA"
                     /locus_tag="ACHX5T_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR8466002.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(7100..7486)
                     /gene="iscU"
                     /locus_tag="ACHX5T_000681"
     CDS             complement(7100..7486)
                     /gene="iscU"
                     /locus_tag="ACHX5T_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR8466003.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7514..8728)
                     /gene="iscS"
                     /locus_tag="ACHX5T_000682"
     CDS             complement(7514..8728)
                     /gene="iscS"
                     /locus_tag="ACHX5T_000682"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR8466004.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8840..9328)
                     /gene="iscR"
                     /locus_tag="ACHX5T_000683"
     CDS             complement(8840..9328)
                     /gene="iscR"
                     /locus_tag="ACHX5T_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR8466005.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9598..10338)
                     /gene="trmJ"
                     /locus_tag="ACHX5T_000684"
     CDS             complement(9598..10338)
                     /gene="trmJ"
                     /locus_tag="ACHX5T_000684"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR8466006.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10457..11260
                     /gene="suhB"
                     /locus_tag="ACHX5T_000685"
     CDS             10457..11260
                     /gene="suhB"
                     /locus_tag="ACHX5T_000685"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR8466007.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11405..12259
                     /locus_tag="ACHX5T_000686"
     CDS             11405..12259
                     /locus_tag="ACHX5T_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR8466008.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12450..13730
                     /gene="csiE"
                     /locus_tag="ACHX5T_000687"
     CDS             12450..13730
                     /gene="csiE"
                     /locus_tag="ACHX5T_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR8466009.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13722..14861)
                     /locus_tag="ACHX5T_000688"
     CDS             complement(13722..14861)
                     /locus_tag="ACHX5T_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR8466010.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(15021..15911)
                     /gene="hcaR"
                     /locus_tag="ACHX5T_000689"
     CDS             complement(15021..15911)
                     /gene="hcaR"
                     /locus_tag="ACHX5T_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR8466011.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            16047..17408
                     /gene="hcaE"
                     /locus_tag="ACHX5T_000690"
     CDS             16047..17408
                     /gene="hcaE"
                     /locus_tag="ACHX5T_000690"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR8466012.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17405..17923
                     /gene="hcaF"
                     /locus_tag="ACHX5T_000691"
     CDS             17405..17923
                     /gene="hcaF"
                     /locus_tag="ACHX5T_000691"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR8466013.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17923..18243
                     /locus_tag="ACHX5T_000692"
     CDS             17923..18243
                     /locus_tag="ACHX5T_000692"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR8466014.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18240..19052
                     /gene="hcaB"
                     /locus_tag="ACHX5T_000693"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18240..19052
                     /gene="hcaB"
                     /locus_tag="ACHX5T_000693"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR8466015.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            19062..20279
                     /gene="hcaD"
                     /locus_tag="ACHX5T_000694"
     CDS             19062..20279
                     /gene="hcaD"
                     /locus_tag="ACHX5T_000694"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFR8466016.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRSAAQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVV
                     DGEKVELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIF
                     AGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQ
                     FIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDA
                     KLLIDENIALKSL"
     gene            20376..20798
                     /locus_tag="ACHX5T_000695"
     CDS             20376..20798
                     /locus_tag="ACHX5T_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR8466017.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20846..21718)
                     /locus_tag="ACHX5T_000696"
     CDS             complement(20846..21718)
                     /locus_tag="ACHX5T_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR8466018.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21730..22824)
                     /locus_tag="ACHX5T_000697"
     CDS             complement(21730..22824)
                     /locus_tag="ACHX5T_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR8466019.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22857..23855)
                     /locus_tag="ACHX5T_000698"
     CDS             complement(22857..23855)
                     /locus_tag="ACHX5T_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8466020.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23880..25391)
                     /locus_tag="ACHX5T_000699"
     CDS             complement(23880..25391)
                     /locus_tag="ACHX5T_000699"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR8466021.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25414..26396)
                     /locus_tag="ACHX5T_000700"
                     /pseudo
     CDS             complement(25414..26396)
                     /locus_tag="ACHX5T_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26493..29774)
                     /locus_tag="ACHX5T_000701"
                     /pseudo
     CDS             complement(26493..29774)
                     /locus_tag="ACHX5T_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            29892..31085
                     /locus_tag="ACHX5T_000702"
     CDS             29892..31085
                     /locus_tag="ACHX5T_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFR8466022.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31283..32536)
                     /gene="glyA"
                     /locus_tag="ACHX5T_000703"
     CDS             complement(31283..32536)
                     /gene="glyA"
                     /locus_tag="ACHX5T_000703"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR8466023.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32863..34053
                     /gene="hmpA"
                     /locus_tag="ACHX5T_000704"
     CDS             32863..34053
                     /gene="hmpA"
                     /locus_tag="ACHX5T_000704"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR8466024.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(34098..34436)
                     /gene="glnB"
                     /locus_tag="ACHX5T_000705"
     CDS             complement(34098..34436)
                     /gene="glnB"
                     /locus_tag="ACHX5T_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR8466025.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34497..35831)
                     /gene="glrR"
                     /locus_tag="ACHX5T_000706"
     CDS             complement(34497..35831)
                     /gene="glrR"
                     /locus_tag="ACHX5T_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR8466026.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35821..36534)
                     /gene="qseG"
                     /locus_tag="ACHX5T_000707"
     CDS             complement(35821..36534)
                     /gene="qseG"
                     /locus_tag="ACHX5T_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR8466027.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36699..38126)
                     /gene="qseE"
                     /locus_tag="ACHX5T_000708"
     CDS             complement(36699..38126)
                     /gene="qseE"
                     /locus_tag="ACHX5T_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR8466028.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38684..42571)
                     /gene="purL"
                     /locus_tag="ACHX5T_000709"
                     /gene_synonym="purI"
     CDS             complement(38684..42571)
                     /gene="purL"
                     /locus_tag="ACHX5T_000709"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR8466029.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
BASE COUNT        10108 a        11798 c        10813 g        10080 t
ORIGIN      
        1 acgttacaga cggtcccggc aaaagtgcct gacctgaccc ctattagcgg ttaccagcgc
       61 cccaccagac ccggtgacat ccccccccgg atcatggacg actgggggca gtaatcatgc
      121 cgggtctggc tggctaacac cccattataa ggggtacgaa taaagtaacg gggacttgca
      181 ggctggcagc gcgatgacct gctattagaa gaaggtgcag tagagctgcc ggaaggagag
      241 tttaacgccg cgtttaatcc tgaagcagtg gtgaaagtaa ccgatgtgtt attggcaagc
      301 catgaaaata cggcgcaaat tattgatgcc cgcccggctg cacgttttaa cgcagaagtt
      361 gatgaacctc gcccaggttt acgtcgcgga catattcccg gtgcattgaa cgttccgtgg
      421 acggaactgg tgcgcgaagg cgaactaaaa acgaccgatg aactggatgc gatatttttt
      481 ggtcgcggcg tcagctatga caaaccgatt atcgtcagct gcggctctgg tgtaacggca
      541 gccgtggttt tgttagcact cgcgacgctg gatgtgccaa acgtgaaact gtacgacggc
      601 gcatggagtg aatggggcgc gcgggcagat ttaccggttg agccagtgaa ataagtatat
      661 tacaggcaat aaaaaaccgc cgaatttggc gtttttttat tgctggtctg gttcgcggcc
      721 tttccagcag gttgacttgt gttacatgag caacgcaggt gcttcacagc aaaacaatac
      781 tcaccagtaa ctctcttttt gtcaagcaaa agagagtaat tattgtttat ttagcgtatt
      841 atcgacaccg gccctttccg ccgtgttcgg taataaaata acctggctta ttagtccgaa
      901 ttcagacaaa tataaacaaa tcctgctcaa aattaaaaat tctaaccggt aaaagatatt
      961 acttaaacat gtaaatttac tttcctttaa aaaacaaaaa accgccaaaa tcaggcggtt
     1021 atttgttgct ggtccggttc gcggcctttc cagcaggttg tattaccgta gtaatgcaag
     1081 cgcgtctcag cggagacaat actcgccagt aactctcttt ttgtcaagca aaagagagtt
     1141 attattgttc tgttagtgta ttatccactg cggccctttc cgccgtctcg caagcgggcg
     1201 ctggcttaag gaaaggatgt tccgcgaccg ttagagcaag cgcgtctcag cgcttgctat
     1261 cgcggcaata tcgccagtgg tgctgtcgtg atgcggtctt cgcatggacc gcacaatgaa
     1321 gatacggtgc ttctgtatcg tacttattgt ttctggtgcg ctgttttccc aagtaaataa
     1381 taaccggagt ctctccggcg acaatttact ggtggttaac aaccttcaga gcagcaagta
     1441 agcccgaatg ccgcccttta gggcggcata ttttagatta tccggttttg tttaaagtca
     1501 cgcaaaaaac caccccagcg acgttcatag aatggcgcaa tatgttcggt aataaagtgg
     1561 ctaattcctt tttccccttt tttcacctga caaatatcga ttggttcatc gccaggtaat
     1621 gtatcggtcg ctacacttcc cgtcgcctga ataatttctt cgatatcacc atcggcttca
     1681 atgccaataa gtaaattagg ctgtgcctct tcgttctctt taattgaaca aataaaagca
     1741 cgcttcaccg gcttaatggt tttaaataag gtggtgagtg aatcaatcat ttgtgctggc
     1801 ggctctgcga cttccgataa tatcagcgat tcaccgcctt ccaggatttc ctggctgctc
     1861 agcggatttc cctcttcacc aatcaacaaa ctgatttcac gcggcataaa ttctttaccg
     1921 gtcggcagtt tggcattaag gaagagcgtt tcgccaaggg tcatctcaaa caatgtgcga
     1981 acgggcatta cgacaaatgc ctgttcgtct tcaaccgcct gttgaagcgc ttctaacgag
     2041 gtgaaaaaag gaatgacgct ggtgccgtct tctttttccc agtgctgtaa atcaagcgca
     2101 ctatcttcaa ccacagcctc gccctgcgcc gccgtaccag gcacccagac ggtggattcc
     2161 agtagagtac ggaaaaaggc cgggcggtgc gccggttcgg ttgctgcttt ttccagcagg
     2221 tcttcaagtt cgttttttgt ttcggacata agaaccacaa ttcattcaac gttcggggcg
     2281 caaatgcctg atgcgctacg cttatcaggc ctacaaggga ttcgcaattt gttgaatttg
     2341 cagaatttgt aggccggata aggcgtttac gccgcatccg gcatattagt ttacgccgtt
     2401 aacagattag ctatcgtgcg cacaccaagt cccgtagcgc ccgcagacca ctgttcaacc
     2461 ggcgctttac ggtaagtcgc cgagcagtcg atatgcagcc agccttgctg atagttctca
     2521 acaaagtgcg acaggaagcc cgccgccgtg ctcgcgcctg ccggatacgc cgcgcttccg
     2581 gtattgttca gttcggcaaa gttagacggc agctggctgc ggtggaattc cgccagcggc
     2641 agacgccaga atggttcgtt ctcttgtgag gcactcgcca gtaaacgacc ggcaagcgca
     2701 tcgtcaaaac tgaacagcgc gtgataatca ttacccagcg cagttttcgc cgccccggtg
     2761 agggtcgccg catcaatgat catttccggt ttctgcgcac tggcatcaat cagaccatcg
     2821 gcaagcacca gacgcccttc ggcatcagtg ttcatcactt caactttttt accgttgcga
     2881 taggtgatga tatcgcccag cttgaacgca ttgccgctaa tcaggttatc cgcacagcag
     2941 aggaacagct tcacgcgctt gttcagtccg cgcataatgg caaatgccag cgccccggta
     3001 accgttgccg cgccgcccat gtccgacttc atcgagtcca taaacgcagt ctgtttgatg
     3061 ctgtagccgc cggagtcaaa agtgatacct ttacctacca ggcacgcgta caccggtgct
     3121 tctttatcgc cagttgggtt gtaatccagc gccagcaata ccggagaacg ttctgaaccg
     3181 cgcccgacag tgtgcagccc catataacct tgctcacgca gatcttcgcc tttggtgatc
     3241 cgataagtca cacgatcgcc cgcgacgttg ctgatcagat caacagcacg ctgtgccagt
     3301 tgcgatggtc ccaattcttc tgccggtgcg ttgatggtgt cacgcaccca gtcgatgatc
     3361 atcaggcggt tatccagttc ctggcgctgg gcatcgtcca gatccggcca ctccacttta
     3421 cgcgtgcctt tcgggccttt ataaccttgc cagaatgccc agcagcgatc cgcatcccag
     3481 ccttcaccgc ttaactgaac atgcttgatg cccagaccgt caatcttgcg ggccgcacgc
     3541 tggatcagcc ccagatcgtc tgccccgttc aaatgcaggg taatgccgtc attattaatg
     3601 ctgtaagttg ctttttctcc ccagcgcgcg tcggcaggtt gggtagagag ggtaatcttc
     3661 atcgcttctg tcattttagt tatccttatt cgtttaacag caaaacgggc tacccccagg
     3721 cagcccgttc attcactcgg cttcgtccag ccagactaac aaaatcgctt cgaggatttt
     3781 ctcgttggat gcctgcgggt cgtcgtcgaa atcttccaga tcgcaaatcc actgatgcat
     3841 atcggtgaat cgaaccgttt tcggatcaag atcgggatac gcatcgtaca gtgcttcgcc
     3901 aatttcgcgg ctatcggtcc acttaagtcc catactaacc tctgttaatg ctcacgcgca
     3961 tggttgatag tgtaacgcgg gatttcgacc actaaatctt cgtcggtgac gcgcgcctgg
     4021 cagcttaaac ggctttccgg ctccagtccc caggctttgt ccagcatgtc gtcttcctgc
     4081 tctgagcttt ccggcagtga gtcaaaacct tcacgaacga tgcagtggca ggtggtgcaa
     4141 gcacaggatt tttcacaggc gtgttcaatc tcaataccgt tacgcagcgc tgcgtcgaga
     4201 atggtttcac cgctattagc ttccagaaca gcgccatcag ggcagaggtc ctgatgaggc
     4261 aaaataacaa tctttggcat attaaacctc gtccacggaa tggcctttca gcgcacgacg
     4321 aaccgactgg tccatgcggc gagcggcgaa atcctgggtt tgtttgtcta cgtttttaat
     4381 cgcttgttcg atggcgtcaa catcatcgcc ctgcgccact tcactcaggt gagcggcagc
     4441 attgtcaatg acctgacgct ctgcggcgct taacagcgcg gcatcagcag ccagcgcgcc
     4501 gtgcagactt tccagcacac gcgccgcttc tactttttgt tctgccagca tacgggcttt
     4561 tacgtcctgc tcggcatagc tcattgagtc tttgatcatc gaagcgattt cgctatcggt
     4621 cagaccgtaa gacggtttga cctgaataga cgcctcaacg ccggtggatt tctccatcgc
     4681 cgtcacgctc aaaagaccgt cggcatcgac ctggaacgtc acgcgaatat gcgcaccgcc
     4741 agccggtagc gccggaatac cacgcagcgc aaaacgcgcc agtgagcggc agtcctgcac
     4801 cagttcgcgc tcaccctgca ttacatggat agacatcgcc gtctgaccat ctttaaaggt
     4861 ggtgaaatcc tgagcgcggg ccaccggaat agtggtatta cgcggaatca ctttctccac
     4921 caggccgccc atcgtttcga ggcccagcga cagtgggatc acatcaagca gcagcatttc
     4981 gctgtctggc ttgttaccca ccagaatatc cgcctgaatc gccgcgccaa tagcgacgac
     5041 tttatccggg tcgatggaag tcagcggtgg acgaccgaaa aattcgccta cccgttcacg
     5101 caccagcggc acgcgagtag aaccgcccac catcaccact tccagcactt cgtcagcttc
     5161 tacacccgcg tctttcagcg cgcgacgaca agccagtaag gttcgtttta ccagtggcgc
     5221 gatcagttca ttgaattgtt cacggctgat ttcgccctgc cagcccgcaa cgttaacggt
     5281 cacggagtcc gcatcgctca gcgcgatttt ggctgcaatg gtggcatcca gcagttcacg
     5341 ctgaacgcgg ttatcgctac gatcaggaat gtccgcctgc tcgcgaatgt aatccgccag
     5401 cagatggtcg aaatcatcgc cgccgagcgc ggaatcaccg ccggttgcca gcacttcaaa
     5461 cacgccgcga cttaagcgca gaatggaaat atcaaacgtc ccgccaccga ggtcataaac
     5521 agcgatcacg ccttcctgac cggaatccag cccgtaggcg atagccgcag cagtcggttc
     5581 gttaagtaag cgcagaacat gcaaccccgc cagacgcgcc gcgtctttgg tgccctgacg
     5641 ctgggcatcg tcaaagtacg ccggaacggt gataactaca ccatccagct cgcctgccag
     5701 ggcttcagtt gcccgcgccg ccagtgcttt gaggatgtcc gcagaaacgc gcaccgggtt
     5761 cagtagcccc gccgccgttt caatcatcgg caggccgttt tcgctggcct ggaattgata
     5821 aggcagatgc ggatagcgtt gctggatatc agccagcgag cgccccatca ggcgtttaac
     5881 agaactaatg gtgttggcgg tgtcaagcgc tgcgttagta cgcgcgtcat aacccaccga
     5941 atgcccttgc tgttgatagt gaacaacaga tggcagcagg tgacggcctt catggtcggc
     6001 taacgtttcg gcctgaccgc tgcgcactgt cgccaccagc gagttggttg tgcccaggtc
     6061 aataccagcc gccagacgac gctgatgcgg cgcagcactc aaaccaggtt cactaatttg
     6121 taataaggcc atgtttagct tccagaaatt aaaaatcgag cagtttttct tcgagttgtt
     6181 cggcactgct ccgcagttta tcgagaaaac gcagcttacg caccgtatcc gccgccgcgt
     6241 cccacgcctc gttgtctaac tgttcaacca tcaactgatg gcgggtatca aacatctttt
     6301 tcacacgttt gataaagctt tccagccgcg cttcatcttt cgcctgttcg atctcgtcca
     6361 gctcttcgcg cagctccaac tgttccatca ggaacgcggt gtcgcgcaca gtatgctgct
     6421 cgctggcgag atcaaaaccg tgtaaagaaa gcaaatattc cgcgcgcatt aacggatgac
     6481 gcagcgtttg ccaggcctgg ttaatggttg cagattgctg tacggcggcg agttgttccg
     6541 ccaggcttcc gctggcgaat ttatcaggat gatactgacg ttgtagatcc tgaaaacgca
     6601 ggctcagtgc ctgggtatcg agttgataac gggcaggcaa gccaaagagg gtgaagtaat
     6661 ccataacatt ctcagggctg cgatccgccg cgataacggc gaatcaggta aaacaaaccc
     6721 cacgcgcagg cgaccacggt ggggttatcg gtatgcgcat caaacgtgga agctttcgcc
     6781 gcaaccacac tcatctttaa cgttcgggtt ggtgaattta aacccttcat tcaggccttc
     6841 ttttacgaag tccagctgcg taccgtccag aaattgcagg cttttgccat cgaccacgac
     6901 tttcacgcct ttgtcttcaa acacgatgtc ttccggcgtc ggttcgtcaa caaattccag
     6961 tacataagcc atacctgaac atccggaggt tctcacgccc agacgcaggc caaacccttt
     7021 gccgcggtta gccagaaagg tatttactcg cgctgctgca ctgtcgctca gtgtaatcga
     7081 cataaccaaa cctcaactct tattttgctt cacgtttgct tttatagtcc gcaatggcgg
     7141 ctttgatcgc gtcttctgcc agaatagaac agtgaatttt caccggcggc agttcaagtt
     7201 cttcagcaat atcggtgttt ttgatcgcct gcgcttcgtc gagagacttc cctttcaccc
     7261 attcggtgac cagggagctg gaagcgatag cggaaccgca gccgtaagtt ttaaaacgcg
     7321 cgtcttcaat gataccttca tcgttgactt taatctgcaa cttcattacg tcgccacagg
     7381 ccggtgcacc caccatgccg ctgccgacgt tctcgtcgtt gttatcaaag gaacccacgt
     7441 tacgcggatt ctcgtaatgg tcgataactt tttcgctgta agccattata aattctcctg
     7501 attccgatac cgattaatga tgagcccatt cgatgctgtt cagatccacg ccctgcttgt
     7561 acatttccca cagcggagaa aggtcacgca gacgaccgat ggatttacga actaactcga
     7621 tggtgtagtc gatctcttct tcagtagtaa aacgacctaa agagaaacgg atagagctat
     7681 gtgccagctc gtcgttcagc cccagcgcgc gcagcacgta ggacggttcg aggcttgctg
     7741 acgtacaggc ggaacctgaa gaaactgcga ggtctttcag cgccataatc agcgactcac
     7801 cttcaacgta gttgaagctg acgttgagaa tgttcggcgc accgtgttcc aggtcaccgt
     7861 tcaggtaaac ttcttcgata tctttgatgc cgttccacag acggttacgc aggccgcgca
     7921 gacgttccat ctcggtcgcc atctcttctt ttgcgatgcg ataggcttcg cccattccga
     7981 cgatctggtg aacaggcaga gtgccggaac gcataccgcg ctcgtgaccg ccgccgtgca
     8041 tttgcgcttc gatgcgtaca cgcggtttac gacgcacata cagcgcaccg atacctttcg
     8101 ggccatagat tttgtgaccg gagaaagaca tcaggtcaac tttcaactgg ctcaggtcga
     8161 ttggcagttt gcccacgctc tgggttgcat caacgtgata gataatgcca cgagcacggc
     8221 acatttcgcc gatagccgcg atatcctgca ccacgccgat ttcgttattt acgtgcatga
     8281 tggaaacaag gatggtgtcg tcacgcatcg ccgcttcgag ttctttcagg tcgataatac
     8341 cgttacgctg cggtgccagg taggtgactt cgaaaccttc gcgctccagc tgacggcaag
     8401 tatccagtac cgctttgtgt tcggttttgc tggtgatgat gtgcttgcct tttttctgat
     8461 aaaagttggc tgcacctttg atcgccaggt tgtcagattc ggttgcacca gaggtaaaga
     8521 cgatttcacg cggatcagcg ccgaccagat cggcaatctg attacgggcg atatctaccg
     8581 cttcttcagc ctgccagccg aaacggtgag aacgagaggc cgggttacca aaggttccgt
     8641 ccatcgtcat gaactgcatc attttctcgg caacacgcgg gtccaccggc gtggttgcgg
     8701 agtagtcgag ataaatcggt aatttcattg ctctataaac tccgtacatc actcaatgca
     8761 aggaatcagg ctaccggctg gatgtacgac cgtgtttacg gagtatttag cactccggcc
     8821 tgattctgaa ttctttttat taagcgcgta acttaacgtc gatcgcgtct tgtgtgcggg
     8881 tgcgtggcgc gtcgtgagta tgctgacgac cagacacatc cagcacttcc tggttattaa
     8941 ccagttcgcc taaagtaatg ttgttgagaa aaccggtgag acggtcgctc aaatcacgcc
     9001 acagcgcgtg ggtcaggcat ttatcgccgc cctggcagcc gcctttaccc tgacaacggg
     9061 tggcatctac agattcgtca acggcgctaa tcacttcgcc aacggcgatg ctgctggcat
     9121 ctttgcctaa cagataacca ccgcctggtc cacgtacgct ggaaaccaga ccatttttac
     9181 gcagacggga aaacagttgt tccagataag aaagggaaat tccctgacgt tcggaaatat
     9241 cagccaacgg taccgggccc gcttcagagt tgagcgcaac gtcaagcatt gcggtcacgg
     9301 catagcgccc tttagatgtc agtctcatgt cttacttcac ctcaaactcg cccctgcccg
     9361 gggtttttta ttgtaaaagt gggggtattg catagcaggg tcaagtctga cattcccgag
     9421 taaattggtc aactatttac ttgactgatt tagtcgggta tttaaccttc agtgccattt
     9481 ttttatcgtg gcgtttgtac ctgttgtcgg atgcggtgtg aacaccttat ccgacctacg
     9541 attctgtaca ttgtaggcct gataagacgc gttaagcgtc gcatcaggca accactgtta
     9601 ttcggccttg ttacctttat tctgctgctc aatagaagcc agaatcccgc gcaggatatt
     9661 caattcctgg ctttccgggc gcgcacgggt aaacagacgg cgcaatttat tcatcacctg
     9721 ccccggatgg ttttcacgga taaaaccggt tgccagcagc gtttgttcca gatgaccata
     9781 aaaacgctcc agatcatcga ccagcggata cggcgtctct tcatgctcga cctgctcgcc
     9841 attttcctga gttgccagcc aggccatacg cacttcatag gcaataacct gaaccgccat
     9901 tgccaggttc agcgaactgt attccgggtt tgccgcaatc gcgacatgat aatggcattt
     9961 ctgcaactct tcattggtca agccgacgcg ctcacgacca aacaccagcg ccaccggggt
    10021 atttgccgct tcagcgacgc ttttcagacc gcattcgcgc gggtcgagca tcggccacgg
    10081 cagcgtgcgg gagcgtgcgc tggtgcccac caccaggcta caaccggcta acgcttcgtc
    10141 gagcgtatcg acgatgtgag cattaccaat cacatcgctg gcccctgcag ccagggcaat
    10201 cgcctgggag tcgggtttca ccagtggatt aaccagccac agattggtta atcccattgt
    10261 tttcatggca cgggcaacag aacccatatt gccggtgtgt gacgtctcca ccagcacaat
    10321 tcgaatattt tgcagcattg tctttcttca tctaaagatt attcacgcat cttatcataa
    10381 aacgaagaca gatgccgatc tcgctgctat actctgcgcc gttttcccgt tctttaacat
    10441 ccagtgagag agaccgatgc atccgatgct gaacatcgcc gtgcgcgcag cgcgcaaggc
    10501 gggtaattta attgccaaaa actatgaaac cccggacgct gtagaagcga gccagaaagg
    10561 cagtaacgat ttcgtgacca acgtagataa agctgccgaa gcggtgatta tcgacacgat
    10621 tcgtaaatct tacccacagc acaccatcat caccgaagaa agcggtgaac ttgaaggtac
    10681 tgatcaggat gttcaatggg ttatcgatcc actggatggt actaccaact ttatcaaacg
    10741 tctgccgcac ttcgcggtat ctatcgccgt tcgtatcaaa ggccgcaccg aagttgctgt
    10801 ggtatacgat cctatgcgta acgaactgtt caccgccact cgcggtcaag gcgcacagct
    10861 gaacggctac cgtctgcgcg gtagcaccgc tcgcgatctc gacggtacca ttctggcgac
    10921 cggcttcccg ttcaaagcaa aacagtacgc cactacctac atcaacatcg tcggcaagct
    10981 gttcaacgaa tgtgcagact tccgtcgtac cggttctgcg gcgctggatc tggcttacgt
    11041 cgctgcgggt cgtgttgacg gtttctttga aatcggtctg cgtccgtggg atttcgcggc
    11101 aggcgagctg ctggttcgtg aagcgggcgg catcgtcagc gacttcaccg gtggtcataa
    11161 ctatatgctg accggtaaca tcgttgctgg taacccgcgt gttgtgaaag ccatactggc
    11221 gaacatgcgt gacgagttaa gcgacgctct gaagcattaa tgactcaggc gggtgatatc
    11281 actcacccgc cctcgccttt caggcgctat tccgaaatac ttcctcaccg ctttactttc
    11341 tttcgtcact ctcccaccat ctttccccga ttaatggatg ataaagaagt cgcaaggaaa
    11401 ggttatggca ctgccagtga acaaacgcgt tcccaaaatt ctgtttattc tctttgttgt
    11461 tgccttctgc gtttatttag tgccgcgcgt tgccatcaac ttcttctatt atcctgacga
    11521 taaaatttac ggtcccgacc cctggtcggc ggaatccgtc gaatttacgg ctaaggacgg
    11581 tactcgtctg caaggctggt ttattccttc ttcgacgggc cctgctgaca acgccatcgc
    11641 aaccatcatt catgctcacg gcaatgccgg aaatatgtcc gcccactggc cgctggtcag
    11701 ttggttacct gagcgtaatt tcaacgtttt tatgtttgat tatcgcgggt ttggtaaatc
    11761 aaaaggcact ccgtcccagg ccggattgct ggacgatacg caaagcgcca tcaatgtggt
    11821 gcgccatcgc agtgatgtaa acccacaaca gctggtgctg ttcgggcaga gtattggcgg
    11881 ggcgaatatt cttgctgtta ttggtcaggg tgatcgtgaa ggcatacgtg cggtgatcct
    11941 cgactccaca tttgcctctt atgcaaccat cgccaaccag atgatccccg gcagtggcta
    12001 cttacttgat gagagttaca gcggcgaaaa ttacatcgcc agcgtcagcc cgatcccgct
    12061 tttactcatt cacggtaaag ccgatcacgt tatcccatgg cagcacagcg aaaagttgta
    12121 tagcctggca aaagagccaa aacggttgat cctaatcccg gatggcgaac acattgatgc
    12181 tttttccgat cgtcacggca atgtttatcg cgaacagatg gtggacttta tccttagcgc
    12241 gttgaatccg cagaactaac ccatgatcgc tagcacgata atcattcaca aaaccacctt
    12301 aagacatgct aatccactgg tcagaacagt ttaaaatgag aaaaattctg tgacgcttgc
    12361 caacatttct gatgattagc attcccttcg ccatttccct gagcaaactt tagctattct
    12421 tatcaattat gcttatggga gatctacaga tgatgcctac gcttgctcca ccatctgtcc
    12481 tttcggctcc ccagcgccgc tgccagatct tgctgacgct ctttcagccg gggttgaccg
    12541 ccaccatggc aaccttcagc gagcttaatg gtgtggatga tgatattgcc agtcttgata
    12601 tcagcgaaac aggacgggag atcctgcgct atcatcaact cacactgacg actggttatg
    12661 acggtagcta ccgggttgaa ggtacagtgc ttaaccaacg tttgtgttta tttcactggc
    12721 tacgacgtgg tttccgtctg tgtccgtcat ttattaccag ccaattcacc cccgccctga
    12781 agagtgaact gaagcggcgc ggaattgcgc gtaactttta cgacgatacc aatctacaag
    12841 cgttagtgaa tctctgctcc cgacggctgc aaaaacgctt tgaatcgcgc gatattcatt
    12901 tcctgtgtct gtatctgcaa tattgtttgc tgcagcacca cgctggaatt acgcctcagt
    12961 ttaatccgct ccaacgtcgc tgggcggaat cctgccttga atttcaggta gcgcaggaaa
    13021 ttggacgcca ctggcagcgt cgggcgctcc agcatgtacc gcctgatgag ccactgttta
    13081 tggcattact tttttccatg ttgcgggttc ccgatccact gcgggatgcg catcagcggg
    13141 acagacaatt gcgtcagtct atcaaacgtc tggtaaacca ttttcgtgag ctgggaaatg
    13201 ttcgttttta tgatgaacag gggttatgtg atcagcttta tatccacctc gcccaggcgt
    13261 taaatcgcag tttgtttgcc atcggtattg ataataccct gccggaagag ttcgccagac
    13321 tgtatccacg cctggtacgc accacccgcg cggcgctggc cggatttgaa agtgaatacg
    13381 gcgtccatct ttctgatgag gaaagtggtc tggtcgcggt gattttcggt gcctggctaa
    13441 tgcaggaaaa cgatctgcat gaaaaacaga ttattctact gaccgggaat gatagcgagc
    13501 gagaagcgca gattgagcag cagttacgcg aactaacgtt actgccgctc aacattaagc
    13561 atatgtcggt aaaggcattt ttgcagacag gcgctccgcg tggcgcggca ctgattattg
    13621 cgccttatac catgccgtta ccgctctttt caccaccgct gatctatacg gacctgacgc
    13681 tgacaacaca tcaacaggag cagatccgca aaatgctcga atcagcatga gggaacaact
    13741 ttcgggcgca aaaacattgc cggaagcgcc accagcgcca ttacccagaa cacgccgtgg
    13801 cccagatatt gatacaggaa accggcgaaa acggtcatga tagcgatact gccgcccatc
    13861 gcgacggcag agtaaaccgc ctgtaaacgg atgacttcgc taacctggcg agcagcaata
    13921 taacgcatgg cggccaggtg gcagaccgtg aaggtgccgc aatgcagaat ttgcaccact
    13981 atcaaccacg gcaacgccgt agttgctccc ataatgcccc agcgcactac gccgcaaatc
    14041 gccgagatca acagcatatc gcgagcacta caacggcgga aaagtttatt actcagcgca
    14101 aagataatga cttccgccac cacgcccagc gaccacaaat accccaccgc cgaggccgag
    14161 tagccagctg cctgccagta aatggcgcta aaaccgtaat aggccgcatg tgccccctgc
    14221 aataaacaaa cgcaggccag aaagcgccag ttctggcgaa ccagcgccaa ccacgcagac
    14281 caaccggtgc tctcctgctg gcggcttgcc ccttgtggct gaatcgtcgg acggatgaga
    14341 aagccgagca gcatggatgc cacgcccaac gtcaacagca cgaggatcac ccgataatca
    14401 aacatagtga ccagtttgcc cgtcagcgcc gagccaatga caaacgccac cgagccccac
    14461 agtcgcactt tgccgtaatc aagcgggaac tgcttttgcc acgtattcgc cagtgcatcg
    14521 gtcaacggta ccagcggtga gaaaaagagg ttaaagccaa tcatcaccag catcagccac
    14581 gctacgtgcg cccccgccca gaaggcgaca gcaaagagaa gtggcagcag tgccagcacg
    14641 cgcaaggcgg aaatcaggcg ggaaggatcg ctgacgcggg gcgcgatgag caaactcccg
    14701 agaaaacggg caaccagacc tgcccccaat aacaggccga tggtttctgg cgttaaacca
    14761 atccctttaa gccagacgct ccagaaaggt aaaaaaatgc cgtaactaaa aaagtatgtg
    14821 aaatagccga gcgccaacca gcgcgtggat tgcaaaacca tgagtccctc ccgtcaggag
    14881 gcgttagtct ggggttaatc gaatgttggt gcaagttgaa aaataagaat attcgagcct
    14941 gcttcccgat gtctgtaacg ccagttcaca agcactaaaa aactgcgatc cgcgacgcat
    15001 gttgcaaaaa taatgcgtaa ttatgccgtt acgcttgcca aacgttcctg cacaatggcg
    15061 atgaaatcgc gtaatgccag cttcatctcc cctttcttcc acgccatcag taaagcaatg
    15121 gaaggcacat ttccggcaat gggacgaaaa acaacctgcc cggtattaaa attattcata
    15181 taaccgggta tcaaagtgac gcccagcccc atgcccacca gattcatggt caccagaata
    15241 ttcgttgcca cctggacgat atttggctgg ctgttttctt gcgcaaacca cgctttaacg
    15301 atcggcgcaa gcgaaccgga atacgccgga tcggtactga cgaaattcac gccatccagt
    15361 tgcgcggcgg tgatctcttt ttcatgtgct aaagggtgat caaccggtaa caccaccact
    15421 aatggttcgt caaaaagctc cagataatca atctccgggc tataaacagg atggcgcatt
    15481 aagccgacgt cgagttcacc acggcgaatt ttttcctcct gttgcgtggt gattaaactc
    15541 accagctcaa tcaaggtgtc tggctgtctg agacgaaaca tcggtaatac ttttggcaat
    15601 aaattcactt ccgccgatgg cacaaagcca atggttaatt gtctgtcttc ctgaacaatt
    15661 ttccgcgccc gtaatttggc attttccgct tgttcgagga ttgccagcgc gtcctggaga
    15721 aaacattctc ccgccgccgt caacgcgact ttgcgcttat ccctcaccag taacggaaca
    15781 ccgacacagt tttcaagatc gcggatctgg ctgcttaacg aaggctgtga ggtatgcagt
    15841 ttttccgccg cacgggtaaa gttcagtgcc tgcgccactg cgacgaaata gcgtaaatgc
    15901 cgtagttcca tcaccttccc cttgttatcg aaaaaacgtc tcagctggta gaaattaact
    15961 atttcacata ttagcaacca accagcaaca tccttatggc acaaaaatag aaggtcaata
    16021 catcttatct ttcaggatta aaaaatatga ccacaccctc agatttgaac atttaccaac
    16081 tgattgacac ccaaaacggt cgggtcactc cgcgtattta taccgacccg gacatttacc
    16141 aactggagct tgagcgtatt ttcggtcgtt gctggctatt tctcgcccac gaaagccaga
    16201 tcccaaaacc cggtgatttc tttaacacct acatgggaga agatgcagtt gtcgtggtgc
    16261 gccagaaaga cggcagcatc aaggcgtttc tcaaccaatg ccgccaccgg gccatgcgtg
    16321 tgagttatgc agattgtggc aacagtcgcg cctttacctg cccgtatcac ggctggtctt
    16381 atggcattaa cggcgagttg atcgatgtac cgctggaacc tcgcgcctac ccacaagggt
    16441 tgtgtaaatc ccactggggg ctaaacgaag ttccttgtgt ggagagttat aaaggactga
    16501 tttttggcaa ctgggatacc agcgcaccgg gcctgcatga ttacctgggt gacattgcct
    16561 ggtatctgga tggcatgctg gatcgtcgcg aaggcggcac cgaaattgtc ggcggcgtac
    16621 aaaagtgggt gatcaactgt aactggaaat tcccggcaga gcagttcgcc agtgaccagt
    16681 atcatgctct gttcagccat gcttctgccg ttcaggtatt aggggcgaaa gatgatggca
    16741 gcgataagcg cctcggtgat ggacaaaccg cccgcccggt gtgggaaacc gccaaagatg
    16801 cgctgcaatt tggtcaggac ggtcacggta gcggtttctt ctttactgaa aaaccggatg
    16861 ctaatgtctg ggtcgatggc gcagtttcaa gctattaccg cgaaacctat gccgaagcag
    16921 aacaacgttt aggtgaagtt cgcgccctgc gcctggcggg tcataacaat atattcccca
    16981 cgctttcatg gctaaacggc actgccacac tccgcgtctg gcatccgcgc ggccctgatc
    17041 aagttgaagt gtgggcgttc tgtattactg acaaagccgc ctccgatgaa gttaaagccg
    17101 cttttgaaaa cagcgccact cgtgcttttg gtcctgctgg ttttctcgag caggatgact
    17161 cggagaactg gtgtgaaatc cagaaattgc ttaaaggcca ccgcgcccgc aacagcaaac
    17221 tgtgtctgga aatggggctt ggtcaggaaa agcgccgcga cgacggcatt cctggcatta
    17281 ctaactatat cttttcagaa actgccgctc gtggaatgta ccaacgctgg gccgatcttc
    17341 tgagtagcga aagctggcag gaagtgctcg ataaaaccgc cgcttaccag caggaggtga
    17401 tgaaatgagt gcgcaagttt cactggagtt acatcaccgc attagccagt ttctctttca
    17461 cgaagccagt ctgctggatg actggaaatt tcgtgactgg ttggcacagc tcgacgaaga
    17521 gattcgttac accatgcgca ccacggttaa cgcgcaaaca cgcgaccgtc gcaaaggcgt
    17581 ccagccgccg acgacctgga ttttcaatga caccaaagac cagctggagc ggcgaatcgc
    17641 ccgtctggaa acgggcatgg cctgggcaga agagccgccg tcacgcactc gtcacttaat
    17701 cagcaactgc caggtaagcg aaaccgacat cccaaacgta tttgccgtga gggtgaatta
    17761 tctactttat cgggcacaaa aagagcgcga tgaaacattc tatgttggaa cgcgtttcga
    17821 caaagttcgc cgtctggaag atgacaactg gcgcttgctg gaacgggata tcgtcctgga
    17881 tcaagcggtg atcacttccc ataacctgag tgtactgttc tgatgaatcg aatttatgcg
    17941 tgtcccgttg cggatgtgcc ggagggtgag gctctccgga tagatacctc gcccgtcatc
    18001 gccttgttca acgtgggcgg cgagttttat gccattaacg atcgttgcag ccatggtaat
    18061 gcgtcgatgt cagaagggta tctggaagat gacgccacgg tggagtgccc gctacacgcc
    18121 gccagttttt gcctgaaaac ggggaaagcg ttatgcctgc ccgccaccga tccgctcacc
    18181 acttatccag tacacgttga aggtggtgac attttcatcg acttaccgga ggcgcagcca
    18241 tgagcgatct gcataacgag tccattttta ttaccggcgg cggatcggga ttagggctgg
    18301 cgctggtcga gcgatttatc gaagaaggcg cgcaagttgc cacgctggaa ctgtcggcgg
    18361 caaaagtcgc cagtctgcgt cagcgatttg gcgaacatat tctggcggtg gaaggtaacg
    18421 tgacctgtta tgccgattat caacgcgcgg tcgatcagat cctgactcgt tccggcaagc
    18481 tggattgttt tatcggcaat gcaggcatct gggatcacaa tgcctcactg gttaatactc
    18541 ccgcagagac gctcgaaacc ggcttccacg agctgtttaa cgtcaacgta ctcggttacc
    18601 tgctgggcgc aaaagcctgc gctccggcgt taatcgccag tgaaggcagc atgattttca
    18661 cactgtcaaa tgccgcctgg tatcctggcg gcggtggccc gctgtacacc accagtaaac
    18721 atgccgcaac cggacttatt cgccaactgg cttatgaact ggcaccgaaa gtgcgggtga
    18781 atggcgtcgg cccgtgtggt atggccagcg acctgcgcgg cccacaggcg ctcgggcaaa
    18841 gtgaaacctc gataatgcag tctctgacgc cggagaaaat tgccgccatt ttaccgctgc
    18901 aatttttccc gcaaccggcg gattttacgg ggccgtatgt gatgttggca tcgcggcgca
    18961 ataatcgcgc attaagcggt gtgatgatca acgctgatgc gggtttagcg attcgcggca
    19021 ttcgccacgt agcggctggg ctggatcttt aaggaagcac gatgaaagaa aaaacgatca
    19081 ttattgtcgg tggcgggcaa gcggcggcaa tggctgcggc ctcgctacgc cagcaagggt
    19141 tcaccggtga gctgcatctg ttttccgatg agcgacatct tccttatgaa cgacctccac
    19201 tctcgaaatc catgttgctg gaagattccc cgcagttaca gcaggtgtta cccgctaact
    19261 ggtggcagga aaacaatgtt catctgcatt ccggtgtaac catcaaaacg ctgggtcgcg
    19321 acacacgaga gttagtgtta accaacggcg aaagctggca ctgggatcag ctttttatag
    19381 caaccggcgc ggcagctcga ccgctgccgt tgcttgatgc actgggagaa cgctgcttta
    19441 ccctacacca tgccggtgat gccgccagac tgcgagaagt tctgcagccc gaacggtcag
    19501 tcgtgattgt cggtgccgga actattggtc tggaactggc tgccagcgcc acgcagcgca
    19561 gcgcagcgca gcgcagatgt aaggtgacag tgattgaact ggcggcaacc gtcatgggcc
    19621 gtaatgcacc accgcccgtg caacgctatc ttttacagcg ccatcagcag gctggtgtgc
    19681 gcattctgct caataatgcc attgaacatg tggtcgatgg tgaaaaagta gaactgacgc
    19741 tgcaaagtgg ggaaacgctt caggctgatg tggtgattta cggtattggt atcagcgcca
    19801 acgagcaact ggctcgcgag gccaaccttg atactgccaa tggcattgtc attgatgagg
    19861 cttgccgcac ctgcgatccc gcgatctttg ccggtggcga tgtggcaatc actcgtcttg
    19921 ataatggtgc actacaccgc tgcgaaagct gggaaaacgc caataaccag gcgcaaattg
    19981 ccgctgccgc aatgttaggg ctaccgctac cgctactgcc gccgccgtgg ttctggagcg
    20041 atcagtacag tgataactta cagtttattg gcgatatgcg tggcgatgac tggctttgtc
    20101 gtggcaaccc ggaaactcag aaggcgattt ggtttaatct gcaaaacggc gtgcttatcg
    20161 gtgcggtcac gctgaatcag ggccgtgaga ttcgcccaat tcgcaaatgg atccagagcg
    20221 gcaaaacgtt tgatgcgaaa ctgctgatag atgagaacat cgcgcttaaa tcactgtaat
    20281 caggataatt agcgaatatc tcaatgcctg gggcgtggcg aggtgcaaga gtgtgtatta
    20341 cgtttaaatc acattatctt gcaaagggat tggttatgaa cacactacgt tattttgatt
    20401 ttggagctgc ccgccccgtt ttgttattaa ttgcccgtat cgccgtggtc ttaattttca
    20461 ttatttttgg ttttcccaaa atgatgggct ttgacggtac ggtccaatat atggcctcgt
    20521 tgggcgcgcc aatgccgatg ctggcagcga ttattgcggt agttatggaa gtgcccgccg
    20581 cgatattaat cgtgcttggc tttttcaccc gtccgctggc ggtgctgttt attttctaca
    20641 cgctgggtac ggcggtgatt ggtcaccatt actgggatat gaccggcgat gcggttgggc
    20701 caaatatgat taatttctgg aagaatgtca gtatcgctgg cgcgttcttg ctattggcaa
    20761 ttaccggacc gggggcaatt tctctcgatc ggcgttagga aagatgccgg atgcggcgtg
    20821 aacgccttat ccggcatttt ataaattaca gccactctac ccgcaacgac atctctgagg
    20881 ttgttgattc ccctggtgct agcgcaatga gatcaccccc ttccgggcga tgatgctcat
    20941 ccggcgcatg gctcatcggt tccagacaga agaaatcaaa cgcatatcct ttatcaaacg
    21001 cagggtcgga aacaaagatg aaataacacg gtgcaggtgg cgtcgtttcc atgatgatgg
    21061 cataaccttc ctgcggctgc tcgatgcggg cctgaccatt ccatccggca aaaccattgt
    21121 tcacccactg acgcggcaac ggcgcgagct ggttaaaatc cagttcctgc ggtagctgct
    21181 cgcaaaactc acccgccagc cactgctccc gttccagcca gtaaccgctc gcctgcgcct
    21241 gaatccgcgt ttgtggtgac aacggaaaat aaggatgcca gccggtacca aatggcagcg
    21301 tctctgcccc ttgattggtg acagagagcg tcaccgtcag cgtatccgcc gttaaatgaa
    21361 acgcctgact tacccgatag tgatagacac cgctgcgatg ttcatacacc agacacaaac
    21421 tatcatcgct gtgcgagaca cattgccact cgcccagcca gccatcgccg tgtagatagt
    21481 gcgcatccca ctcaacattc ggttgcagtt gatactcacg cccctgccag acaaaacggt
    21541 tgccgctcac ccggtttgca aatggcacca gcggaaaaca tgaggcatcg gttgccacac
    21601 cgcttttttt accaggacgt aagagcggcg tcgtatcgcg ccagaagcct tcgattacgc
    21661 cgccctggtc agaaacgtcc agctttagcg acccgtggga taaggtatag atggtcatcc
    21721 gctaactcct taatccggga agttaatcac aactttcccg catttgccgc tcgccatcag
    21781 cacataggca tcacctgcct gttctagcga gaagcgatgg gtaatggcgt tacgcggcca
    21841 cagcttccag tcagtcaaat cgtgggcgca tttttccata tggaacagac tggtcaccca
    21901 ggagccgata atccgccgtt gatggtgcat cagatcagcg ctgacctcga attccacttt
    21961 tccggtttca ccaatgtaaa ccacccgccc ccagtcagca gtggattgca gtgccagcaa
    22021 gcgacctgcg gcattaccgg aacaatcgag cgcaacatcc gcgccaccgt gggtaagttc
    22081 agcaattatc tgcggcagac cttcggtggt tgctaaatag ccgtgatcca tcccccctaa
    22141 ctgttttgcc atcgccagac gttccggcag catatcaacg ccgatgatcc gttttgcacc
    22201 gcgacctttc gccagcatca tcgccatcat gccgaccggc cccagaccga ccaccagcac
    22261 gttatcgctg ccggaaactt cgccgcgcaa aatcccttca taggccgtgc caacgccgca
    22321 actgataaac gcaccatctt cgtagctcag cgcatccggc aggaggatca gatctttttc
    22381 ttccgccagc agatattcgg catgaccgcc gtcacgctgc cagccgtaag ccgcttttcc
    22441 tttgccagta caagagatag gaaagccgcg gcggcagttc gggcaaaaac cacagccaga
    22501 aatgtgatac accagcacgc ggtcgccctc tttaaaatgg cggcagcctt gccccatcgc
    22561 cacaatctgc ccgcacggtt catgaccgtt gataaatccc tggtataacg gtttatcggg
    22621 tgccgccgcc gtggcacggt gttgatgata gatatagtgg acatcgcttc cgcaaatccc
    22681 ggaggatttc attttgatca gtacctggtt aatccccggc gtcggcaccg caacttcccg
    22741 cagatcgacg gttgaatttc ctagtaaata agcagccagc atcgttttca taaatccctc
    22801 attaacaata cgattaattt tcatccctgc agcacccgcg ccaggggcgt taaggtttag
    22861 cgtttcgctt tactgcttcg ctgggtcagc aagatattcg ccagcaccgc caccacgatg
    22921 atgacgccgc gtaccacctg ctggaaaaag gagttaatac cgagcagcac cagaccgtta
    22981 ccgattagcg taatcaccag cacaccaagc aatgtaccga acagggaacc gcgaccgccg
    23041 gaaagtgccg taccgccgac cacgaccgcg gcgatgacgt caaactccag accgtttgcg
    23101 gcacctgcgt taccggaacc gaggcgtgcc gccaacaaaa tgccggtcac cgccgctaat
    23161 aatcccgaaa gggtaaagat aagaatgcgc actcgacgaa cgttaatacc gcacaactgc
    23221 gccgccgtgg cattaccgcc aacggcaaaa accgagcgcc cgaaggcggt ttttcggcta
    23281 atgaacacaa acagcgcaaa caacaccatc atgatcagcg cggatactgg cacaccgaga
    23341 aattgtccgc ccagccagtc cagcacctcg ttttcgtcaa tcggcactgg cagtgcgttg
    23401 gtcataaaca gccccattcc gcgcagagcg ctccacagcc ccagtgtggc aacgaaactt
    23461 ggtacgttaa acacgccgcg cagcaccccg gcgagcgttc ccatcagcgc gcctaacagc
    23521 aacaccagca gacaagcaat cgccaacgga acttcaaatt gcagcaaaaa tgccaggcac
    23581 accgagacaa aagccaccat cggcccaacg ctgacatcaa tttcaccgga gataataatc
    23641 agcgtcatcg cccaggcggc aatcccaatg gtggcggcat cgcgcagcac gttcatctgg
    23701 ttattcaatg agataaagcc aggcgcgttc agggagaaga ccagataaag aatggcaatc
    23761 accaccagca aaccgatctc attaatatgg cgactgacaa attgtttgag cgagacgctc
    23821 ttgccctgcg gcaatggtaa tgacgaagca tacatcttca ggttcctcgg taaaattcat
    23881 cagtgcacag acagaatggc ggacatcagc tcatccacat tgaccggcga gtgaaactcc
    23941 tgcgagaacg tgccgtgctg taataacagg atgcggtcac acaccagcgg caattcctcc
    24001 acttcactgg agataaacac cacgcttttt ccttcggcag ccagctcacg gacaatacgg
    24061 taaatctgct gtttggcttc gatatcgacg ccgcgcgttg gctcgtcgag caacaaaatc
    24121 tggctggcgg catagaccca acgaccgatc accacttttt gctgattgcc accagaaagc
    24181 gtgccgatgg gtgtttcgct actggcagcc ttgaccgtca tccgctgcat cacctcttcg
    24241 gtcaggcggc ggatggtgga ccattgcagc acaccgttgg tgctgatttt ttgccgattg
    24301 gtcagcactg tattttcgtc aacgcctagc cagggaatga tccccgcttc tttgcggttt
    24361 tctggcgtat agccaatgcc gcgtttcagc atgtcgccgt aatcggggcg catgattttc
    24421 tcgccgttga taacaatttc gccctgttca tacgtctcca gcccaacaat cgccttcagc
    24481 aattcactgc gccctgcccc cagcaggcca gcaatgccaa gcacttcgcc acgacgtagc
    24541 gtaaagctga tatcctccag cttgggctta tggcgtaacg cacggacttc cagtacggcc
    24601 tgatccacaa tttcctgagg ggcaaccggc gcaatatcaa cgtgatcacg ccctagcatt
    24661 agcgacacaa tatgatgcgt ggaggtgttt tcgagcatca catcgcccgc cacctgacca
    24721 tcgcgcataa cggtggcata ggaggcaatg cggcggattt cttccatccg gtggctgaca
    24781 taaatcaccg ccacgcccag tgccgacatc tttttcaccg cactgatcac cagttcaact
    24841 tctgcactgg caagcgagct ggtgggttca tcaagaatga ccacgcgcgg ctcgcccttc
    24901 atcacccgtg caatttccac cagttgcttt tgcgccgggc ttagcgttga aacaagttgt
    24961 tcaggactaa cgtcaacgcc cagcgcctgt aagcaacgtt gggcatcctg cgccatttgc
    25021 aggtaatcaa tcatgccgtt gcggcgaggc cattgaccaa ggcagaggtt ttctgccacc
    25081 gtcagccctt ccaccagact taattcctga taaaccgcac gaacccccag ttcagcggca
    25141 cggcgagtca acgtagcttc gtcaccttcc agtcgcgtct cgccaatcca gatatcaccg
    25201 ctatccgggc gttcgctgcc ggtaagcatt cgaatgagag tcgatttgcc cgcgccgttt
    25261 ttacctaaca gcgcacgaac ttcgccttta ttgagcgtga agttaacgtt atccagcgca
    25321 acgacgccgg ggtaacgctt atttcctgcc accacttttg ctaccgggac tgcctctgtt
    25381 gccgtgaaca tagggacctc tgcgaatcag cgattagggc agaccatcaa cgtgcgttgc
    25441 cagccactgt ttaccatctt ccgttttggt atagagatcg attggcacct gaatcacttt
    25501 ttcaccgtcg gcttgtttat tgataacctt caatgtttgc gcgaaaacag cattgcccat
    25561 tttcttaccg gaaatatcca ctaccgcttt cagcacctga ttgttttcca gctcctgagc
    25621 aatttcggtt gtcatatccg aaccgaaaac agcaattttt ccggcctgat tttgattacg
    25681 taccgctttt accgcgccga gtgtcgcacc gcccgactcc cccataatgg cgttgagatc
    25741 cggcgtggag ataatcagtt tttcaccaac ggaaatcgct ttatctaaaa cagtcccttc
    25801 ctggttagcg acaatttgcg cgccgggaac gcgggatttt aatacttctt caaatccttt
    25861 acgtcgctgc acacaaactt caaaggcttc gcaattgatg acggcaattt tcggctggtc
    25921 aattttattg gcaataaaat aatcggcggc agcgttaccc agtttttacc aaattccagt
    25981 ggatcgccga ccagatacgc cgagacatat ttatcgacac ccttttgatt aatacaggtg
    26041 ttgtagcaaa tcaccggaat acccgcttca ctggcgcgac gaacggtacg gctactgcca
    26101 ttttcagaca ctgccgataa aataatggca tcgacattac gcgccacgag ggtatcaaca
    26161 aaggtacttt ctttcgaaat atccccctgg gcgttagttt caattaactg tacctgtact
    26221 gaagaatctt ttgccgcatc ctgaacgccc tgacgcactc cagcgtaata tccctgggta
    26281 tcaaggtata ttgcaccaat ggtcatttct ttatccgcag ccctggcaaa tagtgcgcta
    26341 cctaatagcg tagccattaa taatagatta cgggttgttc tcattttttt aggcatagtg
    26401 cctcctgtag ggtttttatt aacaacggct tattctaatt attttgtgat gagcggcagc
    26461 gcgtgccgct caaatattac agttaatagg gtttagtgaa cgtagttaaa aataaaaggc
    26521 atcacagtgg tgaataatgc cgctttcggc caggccggat taatcgccgt caattcagcg
    26581 cgagcagact catattgcgc aacctccccg agtcccgcgg acgccagcat cgccaccatc
    26641 aggcattttt ctttatgctg ctgttgtaaa tcgccataca gcgataacag gtcaggttgt
    26701 gaaacagcaa agaaatccgc ctcaatactg gttttcgcca tctcttgcgc ccactgtttc
    26761 atttcactaa acagttgctg tgcggtttgc tgttcaccca gcagtcgcag cgccattcct
    26821 tgccagaaga gataatcaac cggctgatcg ttgtaataac tgtggatgtt aatggtgcga
    26881 tcgccggtcg ccgccagacg taaacaacgc atcgcttcag tttcatcgcc ctgcgcgttg
    26941 gcgcatatcg cctgccagaa ccagatgtcg ttatcagttt gccccggtaa acggccttcg
    27001 cttaaattct ccggataata cagcgcggca tgaagcagtt cgctggcctg ctgcggctct
    27061 ctggcatcaa gatgctgcca ggcgcgtaat aactggttga ggataaactg actggtgacc
    27121 ttcccttccc cgccttccca cgggtggaat ttacgcgtgg cgagaatgtc cgccgctttg
    27181 tctgcctgac ccgtgagatg ccacaaattg agcagttctg cggtcatgtc gtcgcgtttc
    27241 agcgcaattt ccagattatt ttccagacgc gccagtcgtt tctccggtgt ggctccactt
    27301 aacttatcca gcaaatcccg ttcgaaaagc agacgtgcat cctgcggcgc aagctgataa
    27361 gcattatcaa gataacgcgc ggccagctca taatcgtgtt tcttattcca cgcatggatc
    27421 gctaacccgc gccagccgtc ggcaaactcc ggcgacatct ctacgcaacg ttgccataag
    27481 gcaatggctt tgttgtagct acgtttgttg tagtagaagc aggccagtaa atggcgagca
    27541 aaccaacact cttcaatact ctccagcgcc gccacttctt ccagcgtatt cgggaaacgg
    27601 acaaactgcg ggaagacatc aatggctttt gcgaccagtt cgccacgttc ggctttcggc
    27661 agcaagctgg cttgcaggta aagcggcagc gtgcgctggc agtccagtgc gttcagcatc
    27721 tctgccgcca gggtgggcat tccccagtta atcagttgcc cggcggtcat cagagcgtta
    27781 acgtcgcgtc cctgacacag accgcgccac tgcgcgaggg ctgattcgct acgaccatcg
    27841 aaccagttca gccaccacag agtggcgttc agcggataat cacgcagcag tttctcgcgc
    27901 tgcaaacgcg cgttgtcctg acgaccactt aacaccagca gcagattatg caagcaaagc
    27961 acttcctgat tggttgggca ggcacgaaga ctttgttggc aaaaatccag acccgcgtcg
    28021 aagttatcat tacgcgccgc cagtcgtgcc agaccataat agccgcctgc tttactattg
    28081 ccgctccaga ccgcacgcca gaaatcctct tcggcttgtt gatattgtcc ctgacgttcg
    28141 taagcactgg cgcgaatcaa acttgcttgt ccacactgcg gttttttgtt cagcgcatgt
    28201 gcacgtttca gcgcctgact ggcatacgcc accgcttgcg ggaaatctgc gcggttatat
    28261 tccagcatcg ccagcgccag gttacagcga taatccagcg ggtccagcgc cacgccgcgc
    28321 aggtagtaat cgaacggtga acggctggcg tgatgatatt gctccagatg ctgaccgata
    28381 aaccaggctt catctgtact ggtaatgtct tgtgctgcca gtggcgcttt ggcgacgtcc
    28441 ggcaacggca actcctgcgg ctgatgttcc tgataactca gtacaatatt gccgtcggca
    28501 tcggagagtt caatggtcag tctgtcagga ttgataccgt gcaacacgcc ctggatggcg
    28561 gtcgctggtg tcagggccac ggcatcatcg agcaacgcgt tgcatttgcc gatttcgcgg
    28621 atcgccagac ggtatccgtt caacggagag atggcataca gcccccactc aatcccccgc
    28681 tcactacgct ggagttttat caccgcatcg cgggaggcat tttgcaccat gcccagagaa
    28741 tgataaggca ggaaatactg ctcgaaacgc ttctcttcgt aagcatcaag ccaggtaaaa
    28801 tcaggctggt tatcggcaaa aataccggtc atcagttcga tatacgggcc gttattgtcg
    28861 gtcagactct tatcccacgc ctggccaaat tcactgtgtc cccaactcca ctgtttttta
    28921 cctggcgcaa tatggtggtt ggcaacgtgc agcaaaccgc catcttcatc gtgacaccac
    28981 gcgccgacaa aatcgtactg cgatttttcg gccatatatg aggttggaac aggcacattt
    29041 ttatagcgag aaatgtccac tccggcggag tagtccactt tgtagtaagt gccggtggcg
    29101 atggggaaag cggagacggc ccgtttgccg tgatcaaaca ccgcagtcac atccggtggg
    29161 aaaacgctct gatgcccttc accccctttc actgccgggt tgacccacca caagaaatga
    29221 cgcggcgtgg cgttgccgtt atagacgcgg ctggcgattt ccagtgccgc ccggtcaggg
    29281 cgcagggtga aacctgtcat cacctgtaaa ccatgcatcg gctccgtttc gccgacccac
    29341 accgtctgtg caccgtcttc atgggcttcg agggtgaaat caacgggcat aaaggtggtc
    29401 gggcgatggt gttgcggcca gttaaactca atcccgccgg agatccacgg tcccagcatc
    29461 cccaccagcg caggtttaat gacttcattg tgatagacaa aatcgcgctg tttcacttta
    29521 tcccatgcgc gatgcacccg accgcccagt tccggcagga tcatcacttt gatgtagtcg
    29581 ttttccagcc acaccgcctg ctaggatttc agggttttct gctcgctcag cgtatcggtc
    29641 acgccgtagg gataaaccgc gccggacgat ccctgataaa cgcgattttc caggaacatg
    29701 ggatgtatat cctgcggccc ggtttcatag gtcgggatct caacgcgctc ttgccacact
    29761 tttactggag tcatggtgcc ctcaatatta acaagacata ctgaattaaa agatttgtgg
    29821 cagtgtattg aacaatctgg caatgttttc gcggaataat cacgcaatta actaaacaag
    29881 gtttagtgaa gatgagagcc tgcattaaca atcaacagat tcgccaccat aacaaatgcg
    29941 tgattctgga actgctgtac cggcaaaagc gcgccaataa atcaacgctg gcccggctgg
    30001 cgcaaatttc gattccggca gtcagtaata ttttgcagga actggaaagc gaaaaacggg
    30061 tggtgaatat tgacgatgaa agccagacgc gcgggcatag tagcggtaca tggctgattg
    30121 cgccggaagg tgactggacg ctgtgcctga acgtgacgcc caccagtatt gagtgtcagg
    30181 ttgctaatgc ttgtttaagt ccgaaaggcg aatttgagta tttacagatt gatgtaccga
    30241 cgccgcaggc gctgctgtcc gaaatcgaaa aatgctggca tcgccaccgt aaattgtggc
    30301 cggaccgcac catcaatctg gcgctggcaa tccacgggca ggttgatccg gtgaccggcg
    30361 tatcgcaaac catgccgcaa gcgccgtgga caacgccagt tgaggtgaag tatctgctgg
    30421 aagagaagct gggcattcgg gtgctggtcg ataatgactg cgtgatgctg gcgctggcgg
    30481 agaaatggca aaataattcg caggaacggg atttctgcgt gatcaacgtt gattacggca
    30541 ttggctcgtc gttcgtgatt aacgagcaaa tttatcgcgg cagtttgtat ggtagcggac
    30601 agattggtca caccatcgtt aatccggatg gagtcgtctg cgactgtgga cgttacggca
    30661 gcctggaaac cgtcgcctcg ttaagtgcat taaaaaaaca ggcgcgggta tggctaaaat
    30721 cacaaccggt taatactcag cttgagcctg aaaaactgac aaccgcgcag ttaatcgctg
    30781 cctggcagag tggagaaccg tggatcacca gctgggttga tcgctctgcc aatgccattg
    30841 gtttgagtct gtataacttc ctcaacatcc tcaatattaa tcagatttgg ttgtacggtc
    30901 gcagttgtgc ctttggtgag aactggctta atactattat tcgccagaca ggatttaacc
    30961 cgttcgaccg cgacgaagga ccgagcgtga aagcgacgca aattggcttc gggcaattaa
    31021 gccgcgcaca acaggtgctg ggaattggct atttgtatgt tgaggcgcag ttacgacaga
    31081 tttgatggcg cgataacgta gaaaggcttc ccgaaggaag ccttgatgat cataaacgaa
    31141 aaattgcctg atgcgctacg cttatcaggc ctacacggag attgcaatat attgaatttg
    31201 caagattttg taggcctgag aaggtgttca tgccgcatcc aacatgaaca acgagcacat
    31261 tgtcagcaaa tcaccgtttc gcttatgcgt aaaccgggta acgtgcgcag atgtcgagaa
    31321 ctttaccttt gatgcgctcg ataacggctt catcattgat gctgtccagc acgtcacaca
    31381 tccagccagc cagttctttc gcttcggctt ctttaaagcc gcgacgggta atcgccggag
    31441 tacctacacg aataccggag gtcacaaacg ggctcttcgg atcgttcggt acgctgtttt
    31501 tgttgacggt gatgttagca cggcccagag cggcgtctgc ttctttaccg gtcaggtttt
    31561 tatcaaccag atcaaccagg aacaggtggt tatcagtgcc gccggaaacc actttgtagc
    31621 cgcgctcgag gaacacttct accatcgctt tagcgttttt cgcgacctgc tgctggtaag
    31681 ttttgaactc aggctccatc gcttctttca gagcaaccgc tttaccggcg attacgtgca
    31741 tcaacggacc gccctgacca ccagggaaaa cggcagagtt cagttttttg tacagctctt
    31801 cgctaccacc tttcgccaag atcaggccgc cgcgcggacc cgccagggtt ttgtgagtgg
    31861 tggtagtaac aacgtgagca tgaggaaccg ggttcgggta gacgccagca gcaaccaggc
    31921 ccgcaacgtg cgccatatca acgaacagat aagcaccgat gctgtcagcg atttcacgca
    31981 ttttcgccca gtcaaccacg ccggaatatg cagagaaacc accgataatc attttcggct
    32041 tgtgttcttt ggcttgtttt tccagatcgg cgtaatcgat atgaccggta gcatcgatac
    32101 cgtaaggaac gatgttgtac agtttaccgg agaagttaac cggagaaccg tgagtcaggt
    32161 gaccgccatg cgccaggttc atacccagaa cggtatcacc tggttccagc agcgcggtgt
    32221 agaccgcaaa gttagcctgg gagccggagt gcggctggac gttagcgtag tcagcgccga
    32281 acagttcttt cgcacgatcg atcgccagtt gttcaacgat atcaacatac tcgcaaccgc
    32341 cgtagtagcg tttgcccgga taaccttcag catatttgtt ggtcagctga gaaccctgcg
    32401 cctgcattac gcgcgggctg gtgtagtttt cggaggcgat cagttcgatg tgctcttcct
    32461 gacgtacttt ttcctgctcc atagcctgcc acagttcggc atcataatcg gcaatgttca
    32521 tttcacgctt taacatccgc atctcctgac tcagctaaca ataaaatttt tggcctttat
    32581 aggcggtcct gttggacaac ggcgaacagt ataaccgaat cattgtgcga taacaggtct
    32641 tgacaaagga atttacgcaa acgattacct tcaggctacg caaggctttg gagaataaag
    32701 agcttgcaac cggaaacgga tttcttttca ggtttgtgat gcaaattttt cacttcatca
    32761 cattctttct gaaaaacacc aaagaaccat ttacattgca gggctatttt ttataagatg
    32821 catttgagat acatcaatta agatgcaaaa aaggaagacc atatgcttga cgctcaaacc
    32881 atcgctacag taaaagccac catcccttta ctggtggaaa cggggccaaa gttaaccgcc
    32941 catttctacg accgtatgtt tactcataac ccagaactca aagaaatttt taacatgagt
    33001 aaccagcgta atggcgatca acgtgaagcc ctgtttaacg ctattgccgc ctacgccagt
    33061 aatattgaaa acctgcctgc gctgctgcca gcggtagaaa aaatcgcgca gaagcacacc
    33121 agcttccaga tcaaaccgga acagtacaac atcgtcggtg aacacctgtt ggcaacgctg
    33181 gacgaaatgt tcagcccggg gcaggaagtg ctggacgcgt ggggtaaagc ctatggtgta
    33241 ctggctaatg tatttatcaa tcgcgaggcg gaaatctata acgaaaacgc cagcaaagcc
    33301 ggtggttggg aaggtactcg cgatttccgc attgtggcta aaacaccgcg cagcgcgctt
    33361 atcaccagct tcgaactgga gccggtcgac ggtggcgcag tggcagaata ccgtccgggg
    33421 caatatctcg gcgtctggct gaagccggaa ggtttcccgc atcaggaaat tcgtcagtac
    33481 tctttgactc gcaaaccgga tggcaaaggc tatcgtatcg cggtgaaacg cgaagagggt
    33541 gggcaggtat ccaactggtt gcacaatcac gccaatgttg gcgatgtcgt gaaactggtc
    33601 gctccggcag gtgatttctt tatggctgtc gcagatgaca caccagtgac gttaatctct
    33661 accggtgttg gtcaaacgcc aatgctggca atgctcgaca cgctggcaaa agcaggccac
    33721 acagcacaag tgaactggtt ccatgcggca gaaaatggcg aagttcacgc ctttgctgat
    33781 gaagttaagg aactggggca gtcactgccg cgctttaccg cgcacacctg gtatcgtcag
    33841 ccgagcgaag ccgatcgcgc taaaggtcag tttgatagcg aaggtctgat ggatttgagc
    33901 aaactggaag gtgcgttcag cgatccgaca atgcagttct atctctgcgg cccggttggc
    33961 ttcatgcagt ttaccgcgaa acagttagtg gatctgggcg tgaagcagga aaacattcat
    34021 tacgaatgct ttggcccgca taaggtgctg taatttgatg ttgccggatg gaaacatccg
    34081 gcaacccttg acgcggttta aattgccgcg tcgtcctctt caccggtacg gatgcgaatg
    34141 acccgagcca cgtcaaagac gaagatttta ccgtcaccga ttttgccggt ttgcgccgtg
    34201 cgaataatgg tatcgacaca ggtatcgaca atgtcgtccg gtacgacaat ctcaattttc
    34261 actttcggca gaaaatccac catatactcc gcgccgcggt acagctcggt atggcctttc
    34321 tggcgaccaa agcctttcac ttcggtcacc gtcatgccgg taataccgac ttcggccagt
    34381 gcttcgcgga catcgtccag cttgaagggt tttataatcg catcaatctt tttcatgcta
    34441 ttccttgaaa aggttgcctg tcttttgatc tgctaaacgt aacacataac gccaattcat
    34501 tccttgaaat cgtttgcatc cagctcgtgt cgggaaagca gtttataaaa ttctgtccgg
    34561 ttgcgccccg ccattctcgc cgcgtgggtg acgttgcctt tggtgatttg cagcagctta
    34621 cgcaagtagt tgagttcaaa atgattacgt gcctcaacaa aggttggcag cgccgtattt
    34681 tcaccctcca gcgcctgctc caccagcgca tcactaatca ccggagatga ggtcagcgcc
    34741 acgcactgtt caatcacgtt gaccaactgg cgcacattac ccggccagct cgcggtcatc
    34801 aggcgtttca tcgcatcggt agagaacgcg cggacaaacg gtttatgtcg ctctgccgcc
    34861 tggcgcaaca ggtgatttgc cagtagcgga atgtcttctg tgcgctccgc cagtgccgga
    34921 attttcaggc tgacaacgtt gaggcggtaa tagagatctt cgcggaattc cccgcgcgcc
    34981 atcgcttttg acagatcacg gtgagtggca gaaataatcc gcacattgat atcaatatcg
    35041 cggttactgc ccagcgggcg cactttacgc tcctgcaaca cgcgcagcag tttgacctgt
    35101 aacggcgcag gcatatcgcc aatttcatcg agaaatagcg ttccgccttc cgccgcctgg
    35161 aataaacctt cgcgattgct gacagcgcca gtaaacgcgc cacgcgcatg accaaacagc
    35221 tccgactcca gcaattgctc gggcaatgcg ccacagttaa tagcaataaa tggtttgctg
    35281 ttgcgcgggc tggcgttgtg gatagcctgg gcgaaaatct ctttcccggt gccgctctga
    35341 ccgttaatca aaacgctgac gtctgattgc gccaccagcc gcgcctgttc cagcaaacgc
    35401 agcatcagcg ggctgcgggt gacaattgcc tcgcgccagc gttcatcggt ggctggcgcg
    35461 gattgctcca gcgcatcgtc aattgcctga tatagcgcat ctttatcgac aggcttggtg
    35521 aggaaactaa aaacgccctg ctgtgttgca gcaacggcat cggggataga accatgcgca
    35581 gtaagaataa ttactggcat tcccggctgc actttctgga tttcagcaaa tagctgcata
    35641 ccgtccattt catccatccg caggtcgctg ataactaaat ctactttttc gcgattcagt
    35701 acccgtaatc cttcagcgcc actttccgcc gtgaccacac tgtagccttc gctggtcagg
    35761 cgcaggccaa gcagtttcag caatcccgga tcgtcatcga ccaataataa atgcgcaggt
    35821 ttatggctca tcaggagtga cctcatgggt ggatggcgcg ggcttttcac tctcgtgcgg
    35881 cgtatccggc gagaaatttc cagccggttt gcgggtcgag agctggcgtt caatatcggt
    35941 caggttttcc agcttgcggg tggtgagttc cagttgctgc tgtagaacgt gatgttgctg
    36001 gcgcaatgta tccagctcgc tgtcgctgga ctgctggagt ttactgtaac gttggcgctc
    36061 ttccgccagt tgcagttgca gcgcctgacc gtcgcgccag agttgataca gtgggcgaac
    36121 ctgtgccggg atctcggtac ttaacgcttc aatacgcgcg accagctggc ggcgctcata
    36181 cggcgtaatt ttggcgtcgg cgagcaaaat cccttgttta aaggtatttt gccagctgcc
    36241 gtcgtcatat tggcgagctt gctgacgcga ctgcgcaggc attaaacgat cagcacaatc
    36301 catcgcccgc agccagtaaa gcggattggt ttcggttgat ttaccttgca gcgcccagat
    36361 gtcgctacat tcagtagaaa gatagtcagc cagttgataa accggaattt tttcttctgc
    36421 tggcgtatca atggctggct tattgtgatt ctgcacgcaa cccagcaatg ccagacatgg
    36481 cagccctgcc agccacagcc gtcggggcaa taatcgttga aaaatgtgtc gcatattcac
    36541 cagacttaaa gcctatccca gtgggcgtaa ttgttgcaga cagtctggac atggacagcg
    36601 cggagaaacc ggggcgtaca tatcgtacgt gaagatttcg agcactaccc ggggccgaac
    36661 tgacaaataa aatagcctga taggataggc tgttagcatt atttcgtgtt tttcgacgac
    36721 ggtaattcaa tgcggaaaca aacgtcttgc ccgctctcgt cgaccagata cagttcccct
    36781 tgcatacggc gaatacaatc cctggcaatg cttaatccca gaccgctgcc cttcaccgcc
    36841 ccttttcgct ggtggcttcc ctgaaaaaag ggttcgaaga tcatggcgcg ttcctcttgc
    36901 ggaatgggcg tgcctgtatt gatgacatca atataaaccc gcgcaccatg taaactgctg
    36961 cgaaggcaaa tgttaccgga ttcagccccg tagtgcaccg cattggagta aagattatcc
    37021 agtacgctca tcagcagcat tggctccgcc aggcaagctg ttgctttgag atcgacgtcg
    37081 gtatgcatca ttttagcccg tgcgggcagg ctatgagcag aaaccactgt ctccaccagc
    37141 ggtgctaatt caacattctc cagttccacc gcactgtccg cctgtttacg gttgtaatca
    37201 agcagttgtt cgatcagttt ttgcaaattg cagctgctgc tatcaagaat gctcaccacc
    37261 tctttttgct ctggcgtaag cggcccgaca acctgatcag ccagtaattc agtgccctcg
    37321 cgcatactcg ccagtggcgt ttttaattca tgagataaat gtcttaaaaa ttgatggcgt
    37381 tgggattcca gccatgacag gcgctcactt aaccaaagaa tacgttgccc aaccgagcgt
    37441 aactcgctcg gtccacagaa cgaaacgcta ttgcccagag aacgcccttc ccccagacgg
    37501 ttgatcatgc gctcgatatt tttcaccggc ccgataatca tccgcgtgaa aagtagcacc
    37561 atcaccagac tcaccagaaa tagcaccagc gattgccaac caaaatattg cccacgttcg
    37621 gcgatttcac gctgaagttg ctgcccacga gagaacacca ctgtgcgcgt ggcctgcacc
    37681 atttcggtat tggcactggc aaaggcttca agacgcgcgg cggcggcagc atcgggacca
    37741 ctgttgttac actgaagttg ggccagattg ttcaaatcct gacgcaatgc ctggtaaagt
    37801 ttatcgtctg gcagtacgcc tgcgtgggca tcgagcattt cgctgtaacg cttgcgctgg
    37861 ctttgataaa ccttcgcaag cgtagggtcg tccagcacgc aatactgacg gtagctacgc
    37921 tccatctcca gcgccgcgtt ggtcatcgct tcactacgcc gggcatcaat aagcgtagtg
    37981 cggttaacca gcgccgcctg atcgctaagc gcattcaggc tttgccaggc ttgccatgcc
    38041 agcaccaaca ggggtagtag aattagcaaa aatgccagca ttaccagttg tcgtaatgag
    38101 cggggaaaaa cgggccagcg tttcaaggtg ttactctcgt cagacgcgaa tagcctgatg
    38161 ctaaccgagg ggaagttcag atacaacaaa gccgggaatt acccggcttt gttatggaat
    38221 aaggcggtgc ctaactcgac gtttcgcccg atggttgata tagctacgct gatatcagaa
    38281 gctggacggc aggcaccttg ttgtgcgtca ttcgaatttt atgtagcacg tcccgaaggg
    38341 gctgacataa gtcggtgaat gagccactgc ttactattat gcagtaactg tgccaataaa
    38401 gaaaatagtt tggtaacgta ctgattatac gttgctttga gggatttatg gcttcttcgc
    38461 tatttgaatg taaatcagcc actgtgtcgc taaaaagaga caacttaaga taaacttatt
    38521 agattgcaat aaaatcaatc atttaagtgt cgccagaaag cgacacggta aaccacccat
    38581 tgtcgtgatt tacagacaca aaaaagctcc cggagttggg agcttatgat agtggttggt
    38641 gcttaatgcc gcatccgggc tgcaaaacca atgaactgat gacttacccc aactgcttac
    38701 gcgcattgcg gaaaatgcgc atccatgggc catcctcgcc ccagttttcc ggatgccagg
    38761 agttgctgac agtacggaaa acacgttccg ggtgcggcat cataatggtg actcgaccac
    38821 tttcagtcgt aaccgccgta ataccgttcg gtgaaccgtt cgggttagcc gggtaggttt
    38881 cagtgacttt gccgaagtta tcgacatagc gcagtgccac cagccctttg ctttccagtg
    38941 ccgccagatg cgccgcatca cgcacttcca cgcgcccttc accatgagag acagcaatcg
    39001 gcatttgcga gcccaccatc ccctgcaaca gcagagacgg gctttgggtt acttcaacca
    39061 ggctgaaacg cgcttcaaag cgatcggagg tgttacgcac aaaacgtggc cacaactcac
    39121 tccccgggat cagttcacgc aggttagaca tcatctggca accgttacat acccccaggg
    39181 ccagcgtttg cggacgatgg aagaaggttg caaactcatc gcgtacacgg tcattgaaca
    39241 ggatagactt cgcccaacct tcaccggcac ccagcacatc accgtaggag aaacctccgc
    39301 acgcgaccag ggcgtgaaaa tcctccagtc ccgtacgtcc agtcagcagg tcgctcatat
    39361 gcacgtcgat agcatcaaag cccccacgtt ggaaagctgc cgccatttca acatgcgagt
    39421 tcacgccctg ctcacgcagt acggcaactt tcggacgtgc gccagtggca atatacggtg
    39481 ctgccacatc ttcgttgata tcgaacgaca gttttacatt caggcccgga tcggcgtcgt
    39541 tagatttcgc ctgatgctcc tgatcggcac actccgggtt gtcacgcagg cgctgcatct
    39601 gccaggtagt ttctgcccac cagacacgca acgtagtgcg gctttcgctg aatacagtct
    39661 gcccgttggc ggtaatcaca aaacggtcac cggaaaccgc ctgcccaaca taatggacgc
    39721 aatccgcaag cccatgctgt gccagtacgg actcgaccgc ttcacggtca gcggcacgaa
    39781 cctgaatcac cgcacccagt tcttcgttaa acaacgccgc caggcgatcg tcacccagag
    39841 aggcgatatc cgcgtcaatg ccacaatgac ctgcaaaggc catttccgcc agcgttacca
    39901 gcaggcctcc atcagagcgg tcgtgatacg ccagcagctt acgctgtgca accagcgcct
    39961 gaatcgcgtc atagaagcct ttcagttgga caacatcgcg tacatctgcc ggtttgtcgc
    40021 caagctgacg ataaacctgc gccagcgccg ttgcgcccag cgcgttattg cctttgccca
    40081 aatcaatcag cagcagtgcg ttatcttcgg tacaaagctg cggcgtgatg gtgtgacgta
    40141 catcttccac gcgggcaaat gcagaaatca ccagcgacag cggcgacgtc atttcgcgct
    40201 cttcgttacc ttcctgccag cgggttttca tcgacataga gtctttaccc accgggatcg
    40261 tcaggcccag cgccggacaa agttcttcac ccacggcttt aacggcttca tacaggcccg
    40321 catcttcacc agggtggcct gccgccgcca tccagttggc ggaaagttta atgcgtttga
    40381 tatcgccaat ctgtgttgcg gcaatattgg ttaacgcttc accgaccgcc agacgggcag
    40441 aggcggcgaa atccagcagc gcaaccggcg cacgctcgcc aatcgccatc gcttcaccgt
    40501 agtagctgtc gaggctggcg gtagtgaccg cgcagttagc gaccggcacc tgccacggcc
    40561 ccaccatctg atcgcgcgcc accatgccgg ttacgctgcg gtcaccgatg gtcaccagga
    40621 aagttttctc cgccacggtc ggcagatgca gtacgcgttt caccgcgtct gcaatggtga
    40681 tcccttcacg gaccagcgca tcgcccttcg ctttcagcgt ttgtacatcg cgggtcatct
    40741 tcggcgtttt accaagcagg acgtccagcg gcagatcgat cggctgatta tcaaaatgac
    40801 gatcgtgcag agaaagatgc agttcttcgg tcgcttcacc aatcaccgcg tagggtgcgc
    40861 gctcacgctt acacagttcg tcaaacagcg gtaattgatc ggcagcaacc gccagcacgt
    40921 agcgttcctg ggattcgtta caccagattt ccagcgggct catgcccggt tcgtcgctta
    40981 gaatatcgcg cagttcaaat ttaccgccgc gtccgccgtc gctcaccagc tccggcatcg
    41041 cgttagaaag accgcccgca ccgacgtcgt ggataaacag aattgggtta gcatcaccaa
    41101 gctgccagca acggtcgatc acttcctggc aacgacgctc catctccggg ttgtcgcgct
    41161 gtacggaagc aaagtcgagg tcggcatcag actgaccaga cgccatggaa gacgccgcgc
    41221 cgccgccaag accgatgttc attgccgggc cgccgagaac gaccagcttc gcaccgacgt
    41281 tgatctcgcc tttttgtacg tgatcggcgc gaatgttgcc gatcccgccc gccagcatga
    41341 tcggtttgtg ataaccgcgc agctcttcgc cattgtggct gttcactttt tcttcataag
    41401 tacggaagta gccgttcagt gccggacgac caaattcgtt gttaaacgcc gcaccgccca
    41461 gcgggccttc ggtcatgatg tccagcgcgg taacaatgcg ctcaggctta ccgaaatctt
    41521 cttcccacgg ctgttcgaag ccaggaattc gcaggttgga tacggagaaa ccaaccaaac
    41581 cagctttcgg cttcgcgccg cgcccggtgg caccttcatc gcggatttca ccgccggaac
    41641 cggtcgccgc gcccggccac ggagaaatcg ccgtcgggtg gttgtgagtt tcgactttca
    41701 tcaggatgtg cgccggttcc tgatggaaat tgtagcggcc cgtttcgtgg tcagcaaagt
    41761 agcggcccac ttcagaacct tccattacgg cggcgttatc tttataagca gagagaacgt
    41821 gatctggcgt ggtttcgaaa gtatttttga tcatcctgaa cagcgatttc ggctgttgtt
    41881 caccgtcgat aatccagtcg gcgttaaaaa ttttgtggcg gcagtgctca gagttcgcct
    41941 gagcgaacat atacagctcg atgtcgttcg ggttacgacc aagctttgtg aaagcatcct
    42001 gcagatagtc aatttcatct tccgccagag ccaagccaag acgcaggtta gcgtcgatca
    42061 gcgcctgacg gccctgcccc agcaaatcaa cgcttgtgac cggagtcggt tgatggtggg
    42121 caaacaactg ctctgcatca tctaaatcaa aaaagaccgt ttccatcatg cggtcgtgca
    42181 gttcagcggt aacctgctgc cattgttcat tggtcagcgt accggcttct atatagtaag
    42241 caacgccgcg ctcaaggcgg tttacctgtt gtagcccgca gttatgggca atatcggtcg
    42301 ctttcgaaga ccagggagag atggtgccag gacgcggggt caccagcagg agtttgcctt
    42361 gcggggcgtg gctggcgagt gccggaccat atttcagcag gcgttcaagt tgtgcgtgct
    42421 catcatcgtt taacggcgca ttgaggtcag caaaatggac atactcggcg taaatattgt
    42481 gaaccgggag cctggcagcc tgaaaacgtg ccagcagttt gttgattcgg aatgccgaca
    42541 gtgcaggcga accacgcaga atttccatca taagtctctc gtcttctaag ctttcggtgt
    42601 acccaagggg ggggaaacgg gcgtcattat aaagaatctg atgcgctgac gaaaccgttt
    42661 gcgtggaaat aaaatcacca tcgtgaatta gcaacgcgtg ccgccaatgg ctgtaataag
    42721 ttgccatctg gcgcaggttt acgcaaaatg ccgctcattt atgagtaaac ctttcactat
    42781 tattacgttt tttcaagct
//
LOCUS       DAZSIO010000015        42597 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZSIO010000015 DAZSIO010000000
VERSION     DAZSIO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.015"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..842
                     /locus_tag="ACHX5T_000710"
     CDS             <1..842
                     /locus_tag="ACHX5T_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466030.1"
                     /translation="IVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRLAFLMTAP
                     EYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDIIISNTGE
                     KFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDTASANRVF
                     HQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKNAECMAHI
                     TFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYDRASGSAS
                     ATQ"
     gene            complement(902..1393)
                     /gene="yajQ"
                     /locus_tag="ACHX5T_000711"
     CDS             complement(902..1393)
                     /gene="yajQ"
                     /locus_tag="ACHX5T_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR8466031.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            1561..2472
                     /gene="panE"
                     /locus_tag="ACHX5T_000712"
     CDS             1561..2472
                     /gene="panE"
                     /locus_tag="ACHX5T_000712"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR8466032.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            2435..3025
                     /gene="yajL"
                     /locus_tag="ACHX5T_000713"
     CDS             2435..3025
                     /gene="yajL"
                     /locus_tag="ACHX5T_000713"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR8466033.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3079..4527)
                     /gene="thiI"
                     /locus_tag="ACHX5T_000714"
     CDS             complement(3079..4527)
                     /gene="thiI"
                     /locus_tag="ACHX5T_000714"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR8466034.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            4733..4975
                     /gene="xseB"
                     /locus_tag="ACHX5T_000715"
     CDS             4733..4975
                     /gene="xseB"
                     /locus_tag="ACHX5T_000715"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR8466035.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            4975..5874
                     /gene="ispA"
                     /locus_tag="ACHX5T_000716"
     CDS             4975..5874
                     /gene="ispA"
                     /locus_tag="ACHX5T_000716"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR8466036.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            5899..7761
                     /gene="dxs"
                     /locus_tag="ACHX5T_000717"
     CDS             5899..7761
                     /gene="dxs"
                     /locus_tag="ACHX5T_000717"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR8466037.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            7816..8733
                     /gene="yajO"
                     /locus_tag="ACHX5T_000718"
     CDS             7816..8733
                     /gene="yajO"
                     /locus_tag="ACHX5T_000718"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR8466038.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(8844..9362)
                     /gene="pgpA"
                     /locus_tag="ACHX5T_000719"
     CDS             complement(8844..9362)
                     /gene="pgpA"
                     /locus_tag="ACHX5T_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR8466039.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9340..10317)
                     /gene="thiL"
                     /locus_tag="ACHX5T_000720"
     CDS             complement(9340..10317)
                     /gene="thiL"
                     /locus_tag="ACHX5T_000720"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR8466040.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(10395..10814)
                     /gene="nusB"
                     /locus_tag="ACHX5T_000721"
     CDS             complement(10395..10814)
                     /gene="nusB"
                     /locus_tag="ACHX5T_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR8466041.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(10834..11304)
                     /gene="ribE"
                     /locus_tag="ACHX5T_000722"
     CDS             complement(10834..11304)
                     /gene="ribE"
                     /locus_tag="ACHX5T_000722"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR8466042.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(11393..12496)
                     /gene="ribD"
                     /locus_tag="ACHX5T_000723"
     CDS             complement(11393..12496)
                     /gene="ribD"
                     /locus_tag="ACHX5T_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR8466043.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(12500..12949)
                     /gene="nrdR"
                     /locus_tag="ACHX5T_000724"
     CDS             complement(12500..12949)
                     /gene="nrdR"
                     /locus_tag="ACHX5T_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR8466044.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13100..13639
                     /locus_tag="ACHX5T_000725"
     CDS             13100..13639
                     /locus_tag="ACHX5T_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR8466045.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            13937..14821
                     /gene="tsx"
                     /locus_tag="ACHX5T_000726"
     CDS             13937..14821
                     /gene="tsx"
                     /locus_tag="ACHX5T_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR8466046.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(14998..15345)
                     /gene="yajD"
                     /locus_tag="ACHX5T_000727"
     CDS             complement(14998..15345)
                     /gene="yajD"
                     /locus_tag="ACHX5T_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR8466047.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(15474..16445)
                     /gene="secF"
                     /locus_tag="ACHX5T_000728"
     CDS             complement(15474..16445)
                     /gene="secF"
                     /locus_tag="ACHX5T_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR8466048.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(16456..18303)
                     /gene="secD"
                     /locus_tag="ACHX5T_000729"
     CDS             complement(16456..18303)
                     /gene="secD"
                     /locus_tag="ACHX5T_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR8466049.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18331..18663)
                     /gene="yajC"
                     /locus_tag="ACHX5T_000730"
     CDS             complement(18331..18663)
                     /gene="yajC"
                     /locus_tag="ACHX5T_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR8466050.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(18686..19813)
                     /gene="tgt"
                     /locus_tag="ACHX5T_000731"
     CDS             complement(18686..19813)
                     /gene="tgt"
                     /locus_tag="ACHX5T_000731"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR8466051.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLLEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(19868..20938)
                     /gene="queA"
                     /locus_tag="ACHX5T_000732"
     CDS             complement(19868..20938)
                     /gene="queA"
                     /locus_tag="ACHX5T_000732"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR8466052.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21031..21604
                     /gene="acpH"
                     /locus_tag="ACHX5T_000733"
                     /pseudo
     CDS             21031..21604
                     /gene="acpH"
                     /locus_tag="ACHX5T_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(21609..23426)
                     /gene="malZ"
                     /locus_tag="ACHX5T_000734"
     CDS             complement(21609..23426)
                     /gene="malZ"
                     /locus_tag="ACHX5T_000734"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR8466053.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(23582..24955)
                     /gene="proY"
                     /locus_tag="ACHX5T_000735"
     CDS             complement(23582..24955)
                     /gene="proY"
                     /locus_tag="ACHX5T_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR8466054.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25031..26350)
                     /gene="brnQ"
                     /locus_tag="ACHX5T_000736"
     CDS             complement(25031..26350)
                     /gene="brnQ"
                     /locus_tag="ACHX5T_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR8466055.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(26757..28052)
                     /gene="phoR"
                     /locus_tag="ACHX5T_000737"
     CDS             complement(26757..28052)
                     /gene="phoR"
                     /locus_tag="ACHX5T_000737"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR8466056.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28110..28799)
                     /gene="phoB"
                     /locus_tag="ACHX5T_000738"
     CDS             complement(28110..28799)
                     /gene="phoB"
                     /locus_tag="ACHX5T_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR8466057.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            28989..30191
                     /gene="sbcD"
                     /locus_tag="ACHX5T_000739"
     CDS             28989..30191
                     /gene="sbcD"
                     /locus_tag="ACHX5T_000739"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR8466058.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            30188..33331
                     /gene="sbcC"
                     /locus_tag="ACHX5T_000740"
                     /pseudo
     CDS             30188..33331
                     /gene="sbcC"
                     /locus_tag="ACHX5T_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
     gene            33457..34638
                     /gene="araJ"
                     /locus_tag="ACHX5T_000741"
     CDS             33457..34638
                     /gene="araJ"
                     /locus_tag="ACHX5T_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR8466059.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(34781..35689)
                     /gene="mak"
                     /locus_tag="ACHX5T_000742"
     CDS             complement(34781..35689)
                     /gene="mak"
                     /locus_tag="ACHX5T_000742"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR8466060.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            35814..36725
                     /gene="rdgC"
                     /locus_tag="ACHX5T_000743"
     CDS             35814..36725
                     /gene="rdgC"
                     /locus_tag="ACHX5T_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR8466061.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(36803..37258)
                     /locus_tag="ACHX5T_000744"
     CDS             complement(36803..37258)
                     /locus_tag="ACHX5T_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466062.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37255..37590)
                     /locus_tag="ACHX5T_000745"
                     /pseudo
     CDS             complement(37255..37590)
                     /locus_tag="ACHX5T_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(37745..38029)
                     /gene="ppnP"
                     /locus_tag="ACHX5T_000746"
     CDS             complement(37745..38029)
                     /gene="ppnP"
                     /locus_tag="ACHX5T_000746"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR8466063.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(38101..38778)
                     /locus_tag="ACHX5T_000747"
     CDS             complement(38101..38778)
                     /locus_tag="ACHX5T_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR8466064.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(39036..39227)
                     /gene="yaiA"
                     /locus_tag="ACHX5T_000748"
     CDS             complement(39036..39227)
                     /gene="yaiA"
                     /locus_tag="ACHX5T_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR8466065.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(39277..39801)
                     /gene="aroL"
                     /locus_tag="ACHX5T_000749"
     CDS             complement(39277..39801)
                     /gene="aroL"
                     /locus_tag="ACHX5T_000749"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR8466066.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(39984..40442)
                     /locus_tag="ACHX5T_000750"
     CDS             complement(39984..40442)
                     /locus_tag="ACHX5T_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR8466067.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            40562..41371
                     /gene="proC"
                     /locus_tag="ACHX5T_000751"
     CDS             40562..41371
                     /gene="proC"
                     /locus_tag="ACHX5T_000751"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR8466068.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(41388..42503)
                     /gene="adrA"
                     /locus_tag="ACHX5T_000752"
                     /gene_synonym="dgcC"
     CDS             complement(41388..42503)
                     /gene="adrA"
                     /locus_tag="ACHX5T_000752"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR8466069.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        10741 a        11370 c        10616 g         9870 t
ORIGIN      
        1 acatagtatt tatcgataaa atttttgcct tttccccatc aacaagaagt gaattaagcc
       61 tcattaattt attaaattca ttaaatgagg tggaattgat gagaaaacgt ctggcgtttc
      121 tgatgacagc accagaatac gcttgttata atagtcagta ttgttatgaa gacaaagtca
      181 gcgcattaaa aatcctgtat cgtcatcatg acaatgagct aatggacaaa tatttcacga
      241 ctcttcttaa agagatcagt aatcaaccat tagaagactg ggatataata ataagtaata
      301 ctggtgaaaa atttagtgaa gtgggtctaa ccaaatatgc tcaacatctc gcatctttcg
      361 ctgaagaatt ttatataaaa gagcaaaaat cacattctga aagtgaaatg agaggactat
      421 tttcatcact tgccgaatta tatagttttg gtaatgatac tgccagcgca aaccgagtgt
      481 tccatcagca tgtccctgcc ttcaatacag atcatatgat tcaatatttt ataaatgcca
      541 aagaatggaa caatgccagg gaattattga taaaagagga aatgttgggt atgcataatc
      601 tgatattgtt ggagaatatt tgtatgcaaa aaaatgcaga atgcatggca cacattacct
      661 ttacgcttaa taaattaaca acacaaccag caataacaaa aatagatagc gtgggtaatg
      721 agcaacttta tcagattggc aaaatctacc ataagttgga aattaaacct gagccagagc
      781 aacaggtttt aattcagtcg ctttatgacc gggcatcagg aagcgcctct gcaactcaat
      841 aaatgcatgg tcaaaagggc caccacggtg gccctgacaa tcagtagcta tatccagaaa
      901 gttaatcgcg gaagtttttg aactggaacg gctgaccgag atcgccaccg cgcaccatcg
      961 ccatgacagc ctgcaaatca tcacgagatt tgccagtcac gcggatctca tcgccctgaa
     1021 tttgcgcctg cactttcagt ttgctgtctt tgatcatctt gacgattttt ttctgagtgg
     1081 cgctctcaat gccctgtttc agtttcgctt ccacaaacca ggttttaccg ctatgaacga
     1141 tattttccgg tacatccagt gaactgcctt caatgccgcg cttcagcagc ttggcacgca
     1201 gaatatccag caactgattg acctggaagt cggactcgct caacactttg atggttttgc
     1261 tggcgtcgtt cagctcaaat gaggcttcaa cgttacggaa gtcaaaacgg gactccactt
     1321 cgcgactcgc gttatcgacc gcgttacgtg cttcctgaag atcaacttca gagacaatat
     1381 cgaaagatgg catcttttcc tctcccttca tttttgatgc gaagcataat acctgcaaag
     1441 ttaaataacc aacagtcctg caccaggacg tatcccttaa ttgtgcatag tcgcaacagc
     1501 atgcacaatt aagggatagc cttattgaac acccggagtg gttgcgggtg aggaggaaca
     1561 atgaaaatta ccgtattggg atgcggtgcc ttagggcaat tatggcttac agcactttgt
     1621 aaacagggtc atgaagttca gggctggctg cgtgtaccgc aaccttattg tagtgtgaat
     1681 ctggttgaga cagatggttc gatatttaac gaatcgctga ccgccaacga tcccgatttt
     1741 ctcgccacca gcgatctgct cctggtgacg ctgaaagcat ggcaggtttc cgatgccgtc
     1801 aaaagcctcg catccacact gcctgtaact acgccaatac tgttaattca caacggcatg
     1861 ggcaccatcg aagaattgca aaacattcag cagccattac tgatgggcac caccacccat
     1921 gccgcccgcc gcgacggcaa tgtcattatt catgtggcaa acggaatcac gcatatcggt
     1981 ccggcacggc aacaggatgg cgattacagt tatctggcgg atattttgca aaccgtgttg
     2041 cctgacgttg cctggcataa caatattcgc gccgagctgt ggcgcaagct ggccgtcaac
     2101 tgcgtgatta atccactgac tgccatctgg aattgcccga acggtgaatt acgtcatcat
     2161 ccgcaagaaa ttatgcaaat atgcgaagaa gtcgcggcag tgatcgaacg cgaagggcat
     2221 catacttcag cggaagattt gcgtgattac gtgatgcagg tgattgatgc cacagcagaa
     2281 aatatctcgt cgatgttgca ggatatccgc gcgctgcgcc acaccgaaat cgactatatc
     2341 aatggttttc tcttacgccg cgcccgcgcg catgggattg ccgtaccgga aaacacccgc
     2401 ctgtttgaaa tggtaaaaag aaaggagagt gaatatgagc gcatcggcac tggtttgcct
     2461 cgcccctggt agtgaagaga ctgaagccgt caccacgatc aacctcttgg ttcgcggcgg
     2521 tatcaaagtc accactgcca gcgtcgacag cgatggcaac ctggcaatta cctgctcgcg
     2581 cggcgtgaag ctgctggcgg atgcgccgct ggtcgaagtg gctgatggcg aatatgacgt
     2641 gatcgtgctg cctggtggca ttaaaggcac ggagtgtttt cgcgatagca ctctgctggt
     2701 tgaaaccgtt aaacagttcc accgttccgg gcgtatcgtc gcggctattt gcgccgcgcc
     2761 agccaccgtg ctggtgccgc acgatatctt tccgattggc aatatgaccg gcttcccgac
     2821 gctgaaagac aaaattcccg ctgaacaatg gcaggacaag cgcgtcgtct gggatgcacg
     2881 ggtaaaattg ctgaccagcc aggggccggg tacagctatc gactttggtc tgaaaattat
     2941 cgacctgctg gttgggcgcg agaaagccca tgaagtggca tcacaactgg tgatggcggc
     3001 agggatttat aattattacg agtagtgtcg gatgcggcaa acgtcgcatc tgaccagatg
     3061 cgacgtaaaa actacaaatt acgggcgata taccttcaca ttgttaaagc cttgctcgcg
     3121 cagatagagc gcctgcagac ggctcatcac cccgcgctca caccacagca gccaggtttt
     3181 gttctggtcg agatcgccaa atttggtgct cagtttatag aacggcagag aaaccacatc
     3241 aatcccttcg actttcagtg gcttatcttc ctgttcatcg acagaacgga tatcgagaat
     3301 cacgtcgttc gggccgaagc cgttgacggt ttccacttcc accacttcct gctcggtctg
     3361 ctgggcgatt tcgcggatat caacgttatt cgcttcctca accactttat cgagaatact
     3421 gaagtcgaac ttctcttctt ccgcttcaat cttcgattta accgctttca ccgtcgggct
     3481 tttggagatc acgccacaat attccggcat cgtgcgagca aagtcttcgg tgccaatctg
     3541 gcgagccagg ttgatgatgt gctctttgtc gtaagaaatc agcggacgca ggatcagcgt
     3601 gtcagagacg ttatcaatca ggcgcaggtt ggtcagcgtc tgactggaca cctggccgag
     3661 cgcttcgccg gtgaccagcg cctgtacgcc gtaacgttca gccactttag atgcggcacg
     3721 caccatcata cgtttgagga taacgcccat ctgaccgtcg tcgattttct cgagaatttc
     3781 cccgacaacc ggttcgaaat taatggcgac aaaacgcacg cggtgggagc tgccaaagcg
     3841 gttccacaga taatgcgcca cctgacgcac gccaatttca tgcgctgcgc cgccgaggtt
     3901 aaagaagcag taatgcacgc ggcagccgcg acgcatcaac atataactgg aaacaccgga
     3961 gtcgaaacca ccggaaatga gcgacagcac atcttcctgg gtgccaatcg ggaaaccacc
     4021 aataccttcg tagcgacctt taatcagcag gaggcgatcg tcttccactt ccagatgaac
     4081 agttacatcc gggttagtca gcttcacgcg cgcggattca atatgctgat ttaaaccgcc
     4141 gccgacgtaa cgttccacat caatcgagct aaaatcatgt ttgccacggc gcttcacgcg
     4201 tacgcagaag gttttgcctt ccagctgatc gcgatactga accaacgctt tctcaaaaat
     4261 atcgtgcatg tcggtaaacg gcacgtcttc gacttcgaga atatggtgga tacccggaat
     4321 acgggtcagg gcgtcgcgaa tagtcagacg ctggttttca tctttcgcgc gaacttcgat
     4381 gttatcccag tggcggacga cagcgagcgt ctcatcatag tgctttaaaa cgttacgaat
     4441 gttcccggta aggattttta taaagcgcaa gcgcacagat tggcttttga tggtgatttc
     4501 cgggaacaat ttaatgataa acttcatggc ggcaatggtt cgttggcaag ccttaagcga
     4561 cttgtatagg gaaaaataca gcagcccaca cctgcggctg catccaggcg cggaagtata
     4621 ccactaacat cgctttgctg cgcacatcac cttaccattg cgcgttattt gctatttgcc
     4681 ctgagtccgt taccatgacg gggcgaaaaa tattgagagt cagacattca ttatgccgaa
     4741 gaaaaatgag gcgcccgcca gctttgaaaa ggcgctgagc gagctggaac agattgtaac
     4801 ccgtctggaa agtggcgacc tgccgctgga agaggcgctg aacgagttcg aacgcggcgt
     4861 gcagctggca cgtcaggggc aggccaaatt acaacaagcc gaacagcgcg tacaaattct
     4921 gctgtctgac aatgaagacg cctctctaac cccttttaca ccggacaatg agtaatggac
     4981 tttccgcagc aactcgaagc ctgcgttaag caggccaacc aggcgctgag ccgttttatc
     5041 gccccactgc cctttcagaa cactcccgtg gtcgaaacca tgcagtatgg cgcattatta
     5101 ggtggtaagc gcctgcgacc tttcctggtt tatgccaccg gtcatatgtt tggcgttagc
     5161 acaaacacgc tggacgcacc cgctgctgcc gtagagtgta tccacgctta ctcattaatt
     5221 catgatgatt taccggcgat ggatgatgac gatctgcgtc gcggtttgcc gacctgccat
     5281 gtgaagtttg gcgaagcaaa cgcgattctc gctggcgacg ctttacaaac gctggcgttc
     5341 tcgattctaa gcgatgccaa tatgccggaa gtgtcggatc gcgacagaat ttcgatgatt
     5401 tctgaactgg cgagcgccag cggtattgcc ggaatgtgcg gtggtcaggc attagattta
     5461 gacgcggaag gcaaacacgt acctctggac gcgcttgagc gtattcatcg tcataaaacc
     5521 ggcgcattga ttcgcgccgc cgttcgcctt ggtgcattaa gcgccggaga taaagggcgt
     5581 cgtgctctgc cagtactcga caagtacgca gagagcatcg gccttgcctt ccaggttcag
     5641 gatgacatcc tggatgtggt aggagatact gcaacgttgg gaaaacgcca gggtgccgac
     5701 cagcaacttg gtaaaagtac ctaccctgca cttctgggtc ttgagcaagc ccggaagaaa
     5761 gcccgggatc tgatcgacga tgcccgtcag gcgctgaaac aactggctga acagtcactc
     5821 gatacctcgg cactggaagc gctagcggac tacatcatcc agcgtaataa ataaacaata
     5881 agtattaata ggcccctgat gagttttgat attgccaaat acccgaccct ggcactggtc
     5941 gactccaccc aggagttacg actgttgccg aaagagagtt taccgaaact ctgcgacgaa
     6001 ctgcgccgct atttactcga cagcgtgagc cgttccagcg ggcacttcgc ctccgggctg
     6061 ggcacggtcg aactgaccgt ggcgctgcac tatgtctaca acaccccatt tgaccaattg
     6121 atttgggatg tgggacatca ggcttatccg cataaaattt tgaccggacg ccgcgacaaa
     6181 atcggcacca tccgtcagaa aggcggtctg cacccgttcc cgtggcgcgg cgaaagcgaa
     6241 tatgacgtat taagcgtcgg gcattcatca acctccatca gtgccggaat tggtattgcg
     6301 gttgctgccg agaaagaagg caaaaatcgc cgcaccgtct gtgtgattgg cgacggcgcg
     6361 attaccgctg gcatggcgtt tgaagcgatg aatcacgcgg gcgatattcg tcctgatatg
     6421 ctggtggtcc tcaacgacaa tgaaatgtcg atttccgaaa atgttggcgc gcttaacaac
     6481 catctggctc agctgctttc cggtaagctt tactcttcac tgcgcgaagg cgggaaaaaa
     6541 gttttctctg gcgtgccgcc cattaaagag ctgctcaaac gtaccgaaga acatattaaa
     6601 ggcatggtgg tgcctggcac gttgtttgaa gagctgggat ttaactacat cggcccggtg
     6661 gacggtcacg atgtgctggg gcttatcacc acgctaaaga acatgcgcga cctgaaaggc
     6721 ccgcagttcc tgcatatcat gaccaaaaaa ggtcgtggtt atgaaccggc agaaaaagac
     6781 ccgatcactt tccacgccgt gcctaaattt gatccctcca gcggttgttt gccgaaaagt
     6841 agcggcggtt tgccgagcta ttcaaaaatc tttggcgact ggttgtgcga aacggcagcg
     6901 aaagacaaca agctgatggc gattactccg gcgatgcgtg aaggttccgg catggtcgag
     6961 ttttcacgta aattcccgga tcgttacttc gacgtggcta ttgccgagca acacgcagtg
     7021 acctttgccg ccggtctggc gattggtgga tacaaaccca ttgtcgcgat ctactccacc
     7081 ttcctgcaac gcgcctatga tcaggtgctg catgacgtgg caattcaaaa gctaccggtc
     7141 ctgttcgcca ttgaccgcgc gggcattgtt ggtgctgacg gtcaaactca ccagggcgct
     7201 tttgatctct cttacctgcg ctgcatacca gaaatggtca ttatgacccc gagcgatgaa
     7261 aacgaatgtc gccagatgct ctataccggc tatcactata acgatggccc gtccgcggtg
     7321 cgctacccgc gcggcaacgc ggttggcgtg gaactgacgc cgctggaaaa actaccaatt
     7381 ggcaaaggca ttgtgaagcg tcgtggcgag aaactggtga tccttaactt tggtacgctg
     7441 atgccagaaa cggccaaagt cgccgaatcg ctgaacgcta cgctggtcga tatgcgtttt
     7501 gtgaaaccac ttgatgaagc gttaattctg gaaatggccg ccagccatga agcgctggtc
     7561 accgtagaag aaaacgccat tatgggcggc gcaggtagcg gagtgaacga agtgctgatg
     7621 gcccatcgta aaccagtacc cgtgctgaac attggcctgc cggacttctt tattccgcaa
     7681 ggaactcagg aagaaatgcg cgccgaactc ggcctcgatg ccgccggtat ggaagccaaa
     7741 atcaaggcct ggttggcata atccctactc cactcctgct atgcttaaga aattattcat
     7801 ggcaggagtg gcagcatgca atacaacccc ttaggaaaaa ccgaccttcg cgtttcccga
     7861 ctttgcctcg gctgtatgac ctttggcgag ccagatcgcg gtaatcacgc atggacactg
     7921 ccggaagaga gcagccgtcc cataattaaa cgcgcgctgg aaggtggcat aaatttcttt
     7981 gataccgcca atagctattc cgatggtagc agcgaagaga tcgttggtcg cgcactgcgg
     8041 gatttcgccc gtcgtgaaga cgtagtcgtt gccaccaaag tgttccatcg cgttggtgat
     8101 ttaccggaag gattatcccg tgcacaaatt ttgcgctcta tcaacgacag cctgcgacgt
     8161 ctcggcatgg attatgtcga tatcctgcaa attcatcgct gggattacaa cacgccgatc
     8221 gaagagacgc tggaagccct gaacgacgtg gtaaaagccg ggaaagcgcg ttatatcggc
     8281 gcgtcatcca tgcacgcttc gcagtttgct caggcgctgg aactacaaaa acagcactgc
     8341 tgggcgcagt ttgtcagtat gcaggatcac tataatctga tttatcgcga agaagagcgc
     8401 gagatgctgc ctctgtgtta tcaggagggc gtggcggtga ttccgtggag cccgctggcg
     8461 cgggggcgac tgacgcgtcc atggggagaa actaccgcac gactggtgtc tgatgaggtg
     8521 gggaaaaatc tctataaaga aagcgatgaa aatgacgcgc agatcgcaga gcggttaacg
     8581 ggcgtcagtg aagaactcgg tgcaacacga gcacaagttg cgctggcctg gttgttaagt
     8641 aaaccgggca ttgccgcacc gattatcgga acttcgcggg aagaacagct tgatgagctg
     8701 ctgaacgcgg tggatatcac tttgaagcca taacagattg ccgagctgga aacgccgtat
     8761 aaaccgcatg cggtcgtagg atttaaataa gagtccaggc ctgatgagac gtgacaagcg
     8821 tcacatcagg catcggtgca caactacgac agaataccca gcggccagtg atgaccgata
     8881 aagtacagga tgcctgcgga aatcaccccg gcgacaatat cgtcgatcat gatccccatg
     8941 ccgccatgta cattgcgatc aaaccagcgg atcggccacg gcttccacat atccagaata
     9001 cggaaaatca caaacccggc ggcaacccac tgccagtcat tggtcggcag cgccatgagc
     9061 gtgatccaca taccaataaa ttcgtcccag acaatgctgc cgtgatcgtg cacgcccata
     9121 tctttcgccg tttgatgaca aagatagacg ccgatacaga tccccaacat caccaccagc
     9181 gagtagagct gccagggtaa aaaggtcatc agataccaga aagggatcgc cgccagcgat
     9241 cccatcgagc caggaacgat tgggcttaat ccacttccga atccgacagc aagcagatgc
     9301 cacggattac tcatcttcag gcgacttttc gcgacatctt tatggcgtga caaaatggtc
     9361 atatcctttc cagtcaaacg tgacaggttc gccgtcacga ataaaacaaa gcccttcgat
     9421 atcggcggtc atttgcccga tacaggtaaa cggtacgcca aggtgtccga gagcaacatc
     9481 cagcgcgcca cggttcagtt ccggcaccgt gaaacacaac tcgtaatctt caccgccaga
     9541 gagcgcccag cgcagcgcct gttccggttc aacatggcga gaaagcgcat cagaaaacgg
     9601 cagcaatgcc aggtcaatac gtgcgccgca gtcgctggct ttcacgatat gcccgagatc
     9661 ggaaatcaga ccgtcagaga gatcgatagc tgaatttgcc agatcgcgca gtgcctgccc
     9721 ctgtaaaata cgcggcgatg gacggagatg acgtttgatc aagtagtcag catctttagc
     9781 atcggcaacc tgcaaacggt tttgcaaaat cgccagcccg gcggcgctat cgcccggtgt
     9841 accggtcaca tagatccagt cacccggttt cgccccagag cgcgttaagg ctcgtcccat
     9901 cggaacaaag ccgtggatac ccaacgtcat tgataatggc ccacgcgtgg tatcgccgcc
     9961 aatgagttgc atatcgtaat aattgagaag atcaaacaaa ctgtcgctga aggactcaag
    10021 ccacgcttcg tctacgtccg gtaaggttaa tgccagcgtc agccaggccg gatcggcccc
    10081 catcgctgcc agatcgctta ggttcaccgc cagtgcttta taagccagat cagcaggatc
    10141 gatatcaggg aggaaatggt tgcccgccac cagcgtatca gtgctgatcg ccagggtctg
    10201 tttctcgggg atattgagaa gagcgcaatc gtcgccgatg cccagttcga catcaagacg
    10261 agaacttctt acacggtcaa aataacgggc aatcagggag aactcgccac atgccatacg
    10321 ttatgcctca gcaggtaaaa aagaaaaggc cggcgatcat ggaacagtct tccgtgaatc
    10381 taccggcctg gatattactt tttgttaggg cgaatcacag gtgctgcttt atcgagtacg
    10441 ccgttgacga acttatggct gtcttctgcg ccgaacgatt tcgccagttc gatcgcttcg
    10501 ttaatggcca ctttgtatgg cacatcacta cgtttagaca gttcgtacag cgcaatgcgc
    10561 agtactgctt tttctacctg gcccagttct tccagcaggc gggacaggta gggcttcatc
    10621 agtccgtcga ggtatgcggt gttagtcgcc accccggcca gcagctcacg gaagtacagg
    10681 acgtcaacgt cttttacatc ctgttcagcc aggaactggt attcaacatc agcgatgtcg
    10741 ttctgggaca actgccagga gtagagcgcc tggacggcac actcacgagc gcggcgacga
    10801 gcagcaggtt tcacggattt ccccttacta atttcaggcc ttgatggctt tcaatacatt
    10861 aatcatttca agcgcggtca gtgcagcttc tgcacctttg ttgccagctt tggtgccagc
    10921 acgttcgatc gcttgttcaa tgctttcagt ggtcagaacc ccaaaagcaa ccggaatttc
    10981 gctgtcctgg gcaacatgcg ccaggccgtt gcttgcacca ccagcgacat attcaaagtg
    11041 ggcagtgcca ccacgaataa ccgtacccag cgcaatcacc gcgtcgtatt taccggtttt
    11101 agccagtgca cccgccgcca gcggcagctc ataggcacca ggcacccaaa caacggtaat
    11161 gttttcatct tttacctgac cgatacgttt cagtgcgtca attgcacctt ccagcaggct
    11221 gtcattgata aagttgttga aacgcgcgat ggtgatggcg acgcgagcgt ccggggtagc
    11281 aacgttagct tcaataatgt tcattttctt ccttcgggtt cgagtatggc cccgcagggg
    11341 ggcggatttt agcataatat ttcgtgcgct gcttcccttt cgagccggga gatcatgcac
    11401 ccactaaatg caggcaaaca tccgggccta catgacgtat ctctttgaat ttaaattggg
    11461 gggcgtcggc taatttctca agccctggca gcgagcataa tccacgggcg tcgctgccta
    11521 atagtttagg tgcgatatag acaatcagct catcgactaa acccgcctgt agcaatacgc
    11581 cagcgagcgt tggccccgct tccacccaga tgctgttaat ctgctgttta cccagttgca
    11641 tcatcagtac aaccagatcc agatgacctt tatgctctgg aatcagcaag gtacgcaccg
    11701 tttccggcca ctcacgagaa tcttcctgcg tacgcgcgaa ccaggtttcg ccgggctgct
    11761 gcacaatgcg atgttccggc gtcacgcgat tttggctatc aatcacaata cgtactggct
    11821 ggcggagatt ttgttgcggg tagagcgcct gagtttgttc atccagttca gaccaacgca
    11881 ctgttaaggc tggatcatcc gccagcaccg tggcgctgct ggttaaaatg gcatgacttt
    11941 gtgcgcgctg tcgttgtacg tcgcgccgcg cctgaggcga agtgatccac tggctttcgc
    12001 cgctcgccat cgccgtgcgg ccatcaagcg atgcgccaag ttttaactga atataaggaa
    12061 agccggtgcg catccgcttg agaaagcctt tattcaattg ctcggcttca ctcatcatca
    12121 gcccgtggct gacgtcaatg ccagcctgtt gcagacggta aagtccacgc ccagcgacct
    12181 gcgggttagg atcttgcatc gctgcgacca cgcgcgctac gccagcggcg atgagtgcgt
    12241 cacagcacgg tggcgtacgg ccatgatggc tacagggttc gagtgtcaca tacgccgttg
    12301 cgcctttggc tttttcaccc gccatacgca acgcgtgtac ttcggcatgt ggttcacccg
    12361 ctcgttggtg gtaaccttca ccgacaattt cgccatcttt gacaatgacg cacccgacat
    12421 tcgggttggg gtgggtggta aaacgtcctc gttgcgccag ctttagcgcc cgcgccatgt
    12481 aatactcgtc ctgcacggct tagtcctcca ggcgcgcgat ctcttcgcca aattctttga
    12541 tatcttcgaa actgcggtag acagaggcaa aacggatata ggcgacttta tcgagctttt
    12601 tcaattgctc catcaccaga ttgccaatca tcttgctcgg tacttcgcgc tcaccggtgg
    12661 cgcgcagctg cgatttaata tgattgattg ccatttcaac gtaatcggaa ctcaccggac
    12721 gtttttccag cgcccgcagc attccgctac gcaatttctc ttcattaaac ggttcacgca
    12781 cgtcgttgct ttttacaaca cgcggcataa ccagctccgc cacttcaaag gtggtgaaac
    12841 gttcattaca caccagacac tgccgacggc ggcgtacgga tgaaccctcg cccacgagac
    12901 gagagtcaat taccttagtg tccacggcga aacagaatgg gcaatgcata cggcgtcctg
    12961 accaggtggt taacggaaat ctattttacc ctgaactgac gatacaacaa aggcgcaacg
    13021 cttttgagat aaaggatcga tgctttcgct gcttttaccc tctaccatta ggctaattgc
    13081 gacgagaaag ggacgaccta tgaatactaa cgtttttcga ctgctcctgc tgggaagcct
    13141 gttcagcctt agcgcctgtg tgcagcaaag tgaagtgcga cagatgaaac acagcgtcag
    13201 cacgctgaac caggagatga cgcaactcaa tcaagaaaca gtcaaaatca ctcagcaaaa
    13261 caggctgaat gcaaaatcca gcagtggggt ttaccttctg cctggagcga aaacaccggc
    13321 aagactggaa agccagatcg gtactttacg tatgtcgctg gtgaatatta cgcctgatac
    13381 agatggcacc acccttacac tacgtattca gggtgagtcg aatgatccct tgcccgcatt
    13441 cagcggaact gttgaatatg ggcaaattca gggaacaata gacaactttc aggaaatcaa
    13501 tgtgcagaat caattgatta atgcccctgc cagcgtcctc gcccccagcg atgttgatat
    13561 tccgttacaa ttaaagggga tttctgtgga tcagttaggc tttgtacgta tccacgacat
    13621 tcaacctgtt atgcagtaaa cgtatttcgg gacgattttg tgcgtcccgc aacatcttcc
    13681 ccgtcatttt gttactctgc ttacatcacc tggattgata gtaaaagttt gcaacaaggg
    13741 cgaaagtcag tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa
    13801 atgatagaac tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct
    13861 ctgtatacga aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc
    13921 aaacagtggc atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc
    13981 ttcgtctttt actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg
    14041 gcaccagagc gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa
    14101 cgatacctac cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta
    14161 tgcggatgcg ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg
    14221 ttctccgctg tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga
    14281 ccttagcttc ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg
    14341 tcgtaataaa gatggtcgcc agagcacctg gtacatgggt ctgggtaccg atatcgacac
    14401 tggcctgccg atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatgg
    14461 cgcagcgaac gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat
    14521 taccgatctg tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc
    14581 cgatttaggg gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc
    14641 tatcgcttcc agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc
    14701 tcgttactgg cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg
    14761 caacttcaac gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg
    14821 attatgaaaa tgccgggatt tattcccggc atttctgatt gttaacaacg tgtgctttgt
    14881 tcatgccgga tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata
    14941 ttgctgaaat aacgtaggcc tgataagcgt agcgcatcag gccattttac gttttaatca
    15001 cttcttcttg ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac
    15061 atctttctgc gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata
    15121 tttcgaatgc tcatgatcgt ggcaatagag acacaacaat tcccagttac tgccatcttc
    15181 cgggttattg gtatggtcgt gatcaatgtg gtgaacggta agttcacgca ggttagaata
    15241 aacaaactcg cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg
    15301 atagccgctt tctaaccgtg catagttttt tgggatgata gccatgaaaa gttacctgga
    15361 tgaaatgtag ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat
    15421 caaaaaaaat cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattaaggc
    15481 agaattgacg gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc
    15541 ttcataccca gtttcagagc caacgcagat gctacgtaga tggaagatgc agtgccgatg
    15601 gaaacaccga taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac
    15661 agcatcagga taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg
    15721 gtcaaggaca cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca
    15781 cgaattcggt ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac
    15841 gatgccacaa tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg
    15901 ataacgtcgt gcgtcagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa
    15961 cctacgtaca cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca
    16021 aggtcagccc ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg
    16081 gtggattcgt taatcacctt cagaacttgg ctgcccagca cctgaccgcc ggtttcgcct
    16141 tcagcaggcg gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc
    16201 tcttcaaaac cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg
    16261 agcgtaattt cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg
    16321 cccataataa cgatagcagc gattaacagc agaccagaga tgccgaaagc ccagtagtcc
    16381 cagcgcataa agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt
    16441 gccacatcgc actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt
    16501 tacgatggca cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt
    16561 agtaatcgcg aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat
    16621 cagcgtggtg atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat
    16681 tgcctgttga acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac
    16741 gttcgcatcg accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag
    16801 tgttgcgcct ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc
    16861 actggtcgca atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag
    16921 cagaccggca aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc
    16981 aattgtacgt tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag
    17041 tgacagctga cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag
    17101 acgagactgg atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc
    17161 atttgcatct ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc
    17221 gatgttgtcc ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt
    17281 aacctgcggc tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccagt
    17341 cagaattacg cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga
    17401 gtcgcccggt acgcgaccgg atgccgcagc ggcctgatca acattggtgt ttaccagacg
    17461 gaattccagc gttgccgtcg cgcccagaat ctctttcgca cgcgcagtgt cctgaatacc
    17521 gggcagttca acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc
    17581 aagttggttt acacggttac gcaggatatt aatgttctgc tgcaccgcat attcacgcgc
    17641 ttcactcaga cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctgctaat
    17701 caccaggtcc ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc
    17761 gcggaaagtg atgctcaggc cgtagttgtt ttctttacga acagtggtat acgggatgcc
    17821 cttttcgcgc aggtcactgc gtaggctatc gatattttgt tcctggagtt tgccaagcgc
    17881 ggtgtccata tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt
    17941 catcggctca gcatgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc
    18001 cacgacgtat ttgtcaccca taacgcccat taatgcttca cgagcgcgca actgggtgtc
    18061 agtggagtcg aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat
    18121 tttttcttct tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc
    18181 gcgcgcacca gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag
    18241 cagaccgatg acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa
    18301 cacggcaatt cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag
    18361 gacggcagct acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc
    18421 aatgtagccg ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac
    18481 ttcatcacct ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg
    18541 tggacgcagg atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga
    18601 catcgggcta ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct
    18661 cattaaattt ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct
    18721 gacgctggta aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta
    18781 aacccgccat caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta
    18841 atatttcgtt gcaacggtcg agatgatgca agtaagcgcg tgaataattg cgacaggtgt
    18901 agcaatcaca ctcaggatcg agtgggccag tatcgctctt atatttcgca ttgcggattt
    18961 tcaccacgcc atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc
    19021 agtcaaacat atcgataccg cgacgtacgc cttcaagcag gtcttccggt ttaccaacgc
    19081 ccatcaggta acgcggtttg tctgccggaa tttgcggaca cacatgctcc agaatacggt
    19141 gcatatctgc tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaagccga
    19201 tatctaccag acctttaaca gatatatcac gtaaatcttc gtaaatactg ccctgaatta
    19261 taccaaataa cgcatttttg ttaccaagac tgtcaaaacg ctcacggcta cgcttcgccc
    19321 aacgcagaga catctccata gagcgttttg cgtaatccca gtcagcagga tacggcgtac
    19381 actcatcaaa gatcatgacg atatccgaac caagatcgta ctgaatctcc atcgactttt
    19441 ccgggtcgag gaaaatcggg tcgccgttga tcgggttacg gaagtgaacg ccctgttcgg
    19501 tgattttacg aatatcacca aggctgaaga cctggaagcc gccggagtcg gtaagaatcg
    19561 gtcctttcca ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg
    19621 ggcgcagcca caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt
    19681 cttccggcgt catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt
    19741 ccactacgcc acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca
    19801 gttcaaattt cattttttct ccactacgtc agaaaaacag tccaacgttt aaaccagcgc
    19861 cgcggaatta ctccccgacg cgctcattaa ttgcctgcgg attgtacgtg ataaacatcg
    19921 catcaccgta actaaaaaag cgatatttct cttctaccgc cgctttatag gcattcatgg
    19981 tgtgttgata accggcaaag gccgaaacca gcataatcag cgtcgactct ggcaagtgga
    20041 agttcgtcac cagcgcatcg accactttgt actggaagcc cggatagata aagatttggg
    20101 tatcgtcaaa gaacggttcg ataagatcgt tttttgccgc ctgtgccgcg ctttccagcg
    20161 aacgtactga agtggtgcca accgcaatca cccggttacc gcgcgctttc gccgccagta
    20221 ccgcgtctac cacatcctgc ggtacttcag cgtattccga gtgcatgatg tgatcttcaa
    20281 tggtgtcgac gcgcaccggc tggaaggtgc ccgcaccaac gtgcaacgtc acaaacgcca
    20341 tctccacgcc tttggcgcgt aatttttcca gcaaaggctc gtcaaaatgc agaccagcgg
    20401 tcggggctgc aaccgcgccc ggtttttcgc tataaacggt ttgataaagt tcgcggtcag
    20461 cgtcttcgtc cggacgatcg atatacggcg gcagcggcat atggccgatg ctgttgagga
    20521 tatccagtac cgagcgttca tcattaaatt cgacttcaaa cagtgcgccg tggcgcgcgg
    20581 tcattgttgc gttgatactt tcgtcatcgc ccagcaacag ttctgcgcca ggttttggcg
    20641 ctttcgaggc gcgaatatgc gcaagaatgc gtttgtcgtc gagcatccgc tcaaccagca
    20701 cttcaatctt gccgccgctg gctttacgcc caaacagacg cgccgggatc acgcgggtat
    20761 tattaaaaac cagaagatcg ccggggttga gcttatcgag taaatcggtg aaagtaccgt
    20821 gcgtcagcgc gcccgtcggc ccgtccagcg acagtaaacg acagctactg cgttcaggca
    20881 tgggatagtg ggcaatcagg gattcgggca attcaaagga gaaatcggta acgcgcatga
    20941 cactgactca gactaaaata agagtcgggt agtctagtgc cggggcgctc tccctgcaac
    21001 ctttaccccc ctttggatag aatataatcg atgaattttt tagctcacct gcatttagcc
    21061 catctcgcgg aaagctcgct ttctggcaat ttactggctg atttcgtacg cggaaatcct
    21121 gaagaaagtt ttccgcccga cgtcgtggct ggcattcata tgcatcgacg tattgactga
    21181 caatctgccg gaagtccgcg aagcacagga gtggtttcgt agtgaaacgc gccgcgttgc
    21241 gcctattacg ctggatgtca tgtgggatca ctttctttcc cgccactggt cgcagctgtc
    21301 gccggacttt ccgctacagg aatttgtctg ttatgcccgc gagcaagtga tgacgatttt
    21361 gccggactca ccgccacgtt ttatcaatct gaacaattac ttgtggtcag agcagtggct
    21421 ggtgcgctat cgcgatatgg atttcatcca gaacgtgtta aacggcatgg caagccgccg
    21481 cccacgtctg gatgccctgc gtgactcctg gtacgattta gacgctcatt atgacgccct
    21541 cgaaacccgc ttctggcagt tttatccgcg gatgatggcg caggcgtcac acaaggcgtt
    21601 ataaaacgtt agttcatcca taccgtagcc gaaatggcag gcaaagccag aatcccgtca
    21661 gtcagttgcc catgcccttc tttgcattgc cattgcacgg cattgagaaa cggtgacgcg
    21721 ggtagcacca cttcactggc ctcgccacgg ttgattgcca ccagtacacg ttgctgattc
    21781 agcacgcgga caaataccac tacgttatct tccgcataca gcacctgaca gccgccacga
    21841 cgcagcgcct gacttttctt acgcagcaca atcattcgcc ggtacagcgc gaataacgcc
    21901 gtatcctgct tttccacctg ccaggggaac ggtttacggc aaaacggatc gtttttacca
    21961 tccagtccca cttcatcacc gtaataaatg cacggtacac caggccaggt gaacagccag
    22021 accaccgcca gcggcaggcg cgcaatatcc cgaccgagca gcgttttaaa tcgcgcagta
    22081 tcgtggctat cgagctgatt aaacatacgt aattgttgtt gatgagaaag ccctgcgcgg
    22141 taattatcca tccaggccat acaggtttgg gcgtcaattt gctgcggatc ataagagatg
    22201 tcggtattgg caagaaatcc ccacaacggg aatgtgaagc cacgatagtt catggcagca
    22261 tcttccacat cggcctgtaa ccattgccgt gcatcgccaa aatgttcgcc gacaatatac
    22321 gcttccggct gggtttcttt cgccgcttcg gtgatcccgg caacgtgctg catattattg
    22381 cgcgccccac ccgcctcccc cagcatatgc accacatcca gccgccagcc gtccatattc
    22441 cacggcgctt tcagccagtg gcggacaata ctgtcttccc cgcgataaat ttcattcacc
    22501 agactttccg actgataatc cagcttcgtc aagctggcat agccaagcca gtcgagcgcc
    22561 gtgccatcat cactaaacga gtaccagtcg cgccagggcg attcagagtt gtgacaagct
    22621 ccccccgtgc caagattgtg cctgtcaaac caggcatggg aatcgccact gtggttaaac
    22681 acgccgtcca gcaccagccg cattcccagc tgctgcgtat tgtgtcgcaa acgcagcaac
    22741 gccccatcac cgccaaactg cggatcgaca tggcgataat cctcggtatc gtatttatgt
    22801 acgctgggag ctttaaacac cggattgaga tacagcgctg tcacgccaag ctttttcaga
    22861 tacggcagtt tttcgcttat cccgtccaga tcgccgccat agaacgttga tccgcccgcc
    22921 tgcgccgtga ccggttcatc ccagtcacgc aagatgatct cttgtccggc tgcatgatgg
    22981 taatagacat gatcctgttc agcttcacga ggaagactac gcgcaaaacg atcagggaag
    23041 atctgataaa aaatctgatc cgcagcccat tgtgggccga tatcaggtac atcgacggca
    23101 aactgctcca gtcgtgccgg cggcattcgg ctgaagccct gcggtgtaaa ccaacgctgg
    23161 cgatcgtgcc acagcagttt gaaactgtaa cgccgccggg gttgtccgct ggagagatca
    23221 atcgccgcac gccatgcggt gacgcctggc tgcggctgac tgcgctgctt atgcattggt
    23281 actgacattt cttcgttatc gtgttctgta cgcagcataa tgcgctgcgg tgggtcttcg
    23341 cccgtcagcc acagtgtaat gagcagttga tctttgcttt gtttaacaaa tgggggcacc
    23401 ggcaggtgcc atgcatttaa catcataatt cccctgtgat gaaaatctga tcaccttgcc
    23461 atagcctaat gcaggatcac tcatcatctg aaggatattt gggggaggag gacgggggcg
    23521 gataactttc ggaatagccg gatcaggatt tcctcctgaa ccggcttaca gaacgcaagt
    23581 attattggtt ttcagccagc tgacgatcgt ggcggcgttt aaacatccag ccaatcaaca
    23641 gcacaacaat ccacgcgaaa ccgacataca gcgagatacg cgtatccggg tgataaccaa
    23701 tcaacccgat aataaagagc aggaaaatca gcccgccgat ggtcgttgct accccacccg
    23761 gcactttaaa tttcagcgcc ttaacttctt ctggcggcaa acggcgacgg aaggcgattt
    23821 gcgacagcag gatcataatc cacacccata ccgtggcgaa ggttgccagc gaagcgatca
    23881 ccaggaagac gttttccggc atgatgtagt tcagatacac cgcaaacagc agcgcggtag
    23941 tcatcaccag caccgtaacc cacggaatac cgcgacgcga cgttttgctg aaaattttcg
    24001 gtgcgctgcc ctgctctgcc ataccgtgga gcatacggcc tacgccaaat acgtcactgt
    24061 taattgccga cagcgaagcg gtcagcacaa caaagttaag aatgctggcg gcaaaggtaa
    24121 tgcccatatg ctggaacgtc agtacgaacg ggctaccggc agtgccaacc tgattccacg
    24181 ggtagataga cataatgacg aacagcgtac caacgtagaa caccagaata cgcatcggca
    24241 cggagttaat cgcacgcgga atcgatttct caggatcttt cgcttcaccg gcggtaatcc
    24301 cgataatttc gatcccaccg taagcaaaca tcaccatttg caacgacatt accatgccaa
    24361 gccagccgtt actgaagaag ccgccgttgc tccacaggtt atgaataccg gtcggttgcc
    24421 cgccgttgcc aatcccccag atgatgatgc cgaaaccggc gacaatcatg atgatgatgg
    24481 tggcgacttt aaagaacgag aaccagaatt ccagctcacc gaagaccttc acgctcatca
    24541 ggtttacggc gcaaatgatc agcaccacgc tcagtaccca aatccagtgc ggcaccgtcg
    24601 ggaacaagac acccatatag ataccaaaag cggtcacatc ggcgatggcg acaataagga
    24661 tttcaaaaca gtaggtccat ccggtaatgt aacctgccag cgggccaagg ttttcctgcg
    24721 cataacgcga gaaagagctg gcggccgggt tatgtaccga catttccccc agcgcacgca
    24781 taatgatata cgccgcgata ccaccgataa tataggccaa caacacgctc ggaccggcca
    24841 ttttgatggc gtctgccgaa ccgtaaaaca gcccggtgcc aattgctgaa cccagtgcca
    24901 taaagcgtat gtggcgggta cttagcccac gctttagctt attcttactt tccatcaatt
    24961 cctaacccat caacacaata aaaaaccacg gggcatcgca gccccgtggt taaaacaaat
    25021 gttcagtgat ttagtgagcg ctggaggtca cctgacgacc tgccgcgcga tcccagataa
    25081 tggccagaac caccatcacc actgttggca ttaaccacgc cagaccttgt tcggccagcg
    25141 gtaaacgctg cgcccaggac ggtaagatat cgctgaatgc agatgctttg atcccgtcga
    25201 gaataccaaa aagcaggctg ataaacatcg gcggagcaat cacgcgggac gaattatgcc
    25261 accatgagcg tgtaaaactt aatacaacca gtgcgataca cggcggataa atagcggtca
    25321 gcaccggtac ggagatctgg atcagctggc ttaagccgag gttagacacc accatcgaga
    25381 agccgccgag gataaacacc agcgtacgat aagagagcgg tacgtactgg gcaaagaatt
    25441 ctgcacaagc acaggtcagg ccaaccgccg tgaccagaca ggcgatgaag attaacgccg
    25501 ccagcaggaa gctaccgccg ccgccaaagg tgtgctgaac gtaagcatgc agaatagcag
    25561 cgccgtttgc agactgatcg accagcgacg cgctgtctga acccagacag aacagcgcca
    25621 ggtacagcaa agtcagacca acacccgcca tcaggccagc ccagacggta taacgggtca
    25681 gcagacgcgc ttcggtaacg ccacgagaac gcgccgcgtt aacaataacg ataccaaaca
    25741 ccattgcgcc cagcgtatcc atggtcagat agccgttaac gaagccgtta gaaaatgcag
    25801 cgttttgata agcctcagtc gccgtgctga tagaacccgc cggccagaca atcgcgacaa
    25861 cagacaggat gaccagcgcg ataattttca gcggcgcaag gaagttgccc acggtatcca
    25921 gcagcttgcc cggatagagc gaaaccagaa taacgatagc gaaatagacc aggctgtaga
    25981 taaacagcgg cagcgcggaa tcacccgtca gcggcgcaat ccccacttca aaggaaacgg
    26041 tagctgtacg cggcgtagcg aaaagcggcc ccaccgccag gtaacaaacc gttgccagca
    26101 gtacgccagc gactttaccg attggcgtgc tgaggctgtc aacaccgccg ccaacttttg
    26161 ccagcgccac taccgttaat accggcaggc caacggcagt aatgaggaag ccgaatgccg
    26221 cagtccagac gtgttcgcct gcctgtaagc cgaccattgg agggaaaata atgttacctg
    26281 cgccgacgaa caacgcaaat gtcataaagc ccagagcgat gatatcgcgc gatcttaatt
    26341 gatgggtcat aaatcatact gcctgtggat gtggtgttgt aacgttaaat ttttgtgcca
    26401 atccctgggg atgatacagc ggtatattta aacaatttta gcgggaaata ctcccaacca
    26461 ggcgcggcga agaatagttt ttcgatttac cgcgttaatg cagtcagtat gacagcacct
    26521 gaagcgcaat ttaaacgctt ataacgtttt aaagcaagga gggatctaaa aaccagaggt
    26581 atatagcgat aagaaatcac caaccagtat aaaacactat ttccgtgaaa aagacatggc
    26641 taatcatgcg aacaaaaaat caccaaccgt tgtaaaatct aacgcccgta ttttgactgc
    26701 aaagaaaata agccagcatc ggctggctta tggcaataaa agatgacaaa ggcggattaa
    26761 tcgctgtttt tggcaattaa acgttccggg ataacaaaac tgaaacgtgt tccttttcct
    26821 actgtactct caatattcag gcgactttcg tgatgattca ccgcatgttt cacgatcgct
    26881 aaccctaatc cgctaccgcc ggtttgccgg gaacgcgctt tatcaacgcg ataaaaacgc
    26941 tcggtcaggc gcggaatatg ctccggtgca atgcccggtc cgttatcttc aacgctaaat
    27001 tcggcaccgt gcggcactcg ctgccagcgt acggtgatat gcgtgccttc cggcgtatga
    27061 ttcacggcgt tatagaccag gttcgaaatc gcactgcgta gctgatcttc gttgccagac
    27121 accttgaggc cgttatctat ctcaaaggta aatgtctgtt ttttctgact cagagtctga
    27181 gcctcgcgct caacaacgcg cagcatcatc ggcacatcaa ccttttcatt gagcaattgc
    27241 gtcggcgcgg cttcaatttt cgacagcgtt agcaactgct tcaccagccc ttccatccgc
    27301 tgggtctgct cgcgcatggt gtgcaacgct ttttcgcgta ccgcgccttc cagcggctgc
    27361 tcatccatca tctccaggta accctgtaac acggtcaatg gcgtacgtaa ctcatggctc
    27421 acgttggcaa aaaagttacg ccgcgcccct tccagttgat gcatttgcgt gacatcacgc
    27481 gccaccatca gcaactgttt gtgggtataa ggcatgacgc gaatttccag atgccgcccg
    27541 gtgttgagca ccagattgag cgggcgagaa aaatcacgcg ttttcagata ttgcgtaaac
    27601 tccgggtaac gcagtaggtt aaggatgttc tgcccgttat cttccggcca gcgcaaacca
    27661 agaatttgtt gcgccagacc gttacaccag aaaataccgc cctcttccgt ggtcagcacc
    27721 accgcgtcgg gcagcgactc cgcgccgcta cgaaagcgtt taatcagatt gcccagttca
    27781 cggcggcgtt ttttatttcg cagctgcatc tggtgtaagc cgtatagtag cggttcccag
    27841 ctaccacgcc ccggtggcgg ggtcatactg cgatccaccc acagccacca tgaaaggcgc
    27901 aataaattcc agaaatgcca gataagcagt cctgttaccg atgccagcaa aaaccagggc
    27961 aggtaaccaa aaaatgcacc caggatgaaa gccgggaggc agcaaagtag cagctccagc
    28021 accagccttt tccacgacag ccgttccagc acgtaagata ctccagttaa gaaatcataa
    28081 gccctgctct gcgtccgatg agcaaggcgt taaaagcggg ttgaaaaacg atatcctgta
    28141 ccgcgcacgg tctgcaccat gcggtcatgc ccgccgggct ccagtgcttt acgcaggcga
    28201 cgaatgtgga catcgaccgt gcggtcttcc acatacacgt tagttcccca gacgtggttt
    28261 aacagctgct cgcggctgta cacgcgctca ggatgcgtca taaaaaagtg cagcagttta
    28321 aattctgtcg gccccatctc cagcggctct tcgcccgcca tcactcggtg agatgtcggg
    28381 ttgagactta atccctgcat cttaatcacc tcttccaccg ccattggcga aatacggcgc
    28441 attaccgctt tgattcgcgc caccagctcc ttcggcgaaa atggcttggt gatatagtca
    28501 tccgcgccgg tttcaaggcc gcgcacgcga tcttcttctt cccctctggc ggtcaacatc
    28561 accactggaa tatcccgggt catcgactcg cgcttgaggt gtttgatgaa ctggataccg
    28621 gagccgccag gtaacatcca gtcaaggaga attaaatccg gccagggttc attcagttga
    28681 ttcacagcac tgtcataatc ttccgcttcg accggctgaa agccattttg ttcgagcacg
    28741 aagcagacca tttcgcgaat tggagcttca tcttctacga ccagaatacg tctcgccatg
    28801 atttgccctg ttgtaataaa taggttgcga tcattaatgc gacgtcatta tgcgtcagat
    28861 ttatgacaga tttatgaaaa gctcgtcgca catatcttca ggttattgat ttccgtggcg
    28921 cagaaaaaag caaatggcac atctgtttgg gtataatcgc gaccatgctt tttcgccagg
    28981 gaaccgttat gcgcatcctt cacacctcag actggcatct cggccagaac ttctacagta
    29041 aaagccgcga agctgaacat caggcttttc ttgactggct gctggagaca gcacaaaccc
    29101 atcaggtgga tgcgattatt gttgctggtg atgttttcga taccggctcg ccgcccagtt
    29161 acgcccgcac gttatacaac cgttttgttg tcaatttaca gcaaactggc tgtcatctgg
    29221 tggtactggc aggaaaccat gactcggtcg ccacgctgaa tgaatcgcgc gatatcatgg
    29281 cgttcctcaa tacaaccgtg gtcgccagcg ccggacatgc gccgcaaatc ttgcctcgtc
    29341 gcgacgggac gccaggcgca gtgctgtgcc ccattccgtt tttacgcccg cgtgacatta
    29401 tttccagcca ggcggggctt aacggtattg aaaaacagca gcatttactg gcagcgatta
    29461 ccgattatta ccaacaacac tatgccgatg cctgcaaact gcgcggcgat cagcctctgc
    29521 ccatcatcgc cacgggacat ttaacgaccg tgggttccag taaaagtgac gccgtgcgtg
    29581 acatttatat tggcacgctg gacgcgtttc cggcacaaaa ctttccacca gccgactaca
    29641 tcgcgctcgg gcatattcac cgcgcacaga ttattggcgg catggaacat gttcgctatt
    29701 gcggttcccc cattccactg agttttgatg aatgcggtaa gagtaaatat gtccatctgg
    29761 tgacattttc aaacggcaaa ttagagagcg tggaaaacct gaacgtaccg gtaactcaac
    29821 ccatggcggt gctgaaaggc gatctggcgt cgattaccgc acagctggaa cagtggcgcg
    29881 atgtatcgca ggagccacct gtctggctgg atatcgaaat cactactgat gagtatctgc
    29941 atgatattca gcgcaaaatc caggcattaa ccgaatcatt gcctgtcgaa gtattgctgg
    30001 tacgtcggag tcgtgaacag cgcgagcgtg tgttagccag ccaacagcgt gaaaccctca
    30061 gcgaactcag cgtcgaagag gtgttcaatc gccgtctggc actggaagac ctggatgaat
    30121 cgcagcagca acgtctgcag catcttttca ccacgacgtt gcgtaccctc gccggagaac
    30181 acgaagcatg aaaattctca gcctgcgcct gaaaaacctg aactcattaa aaggcgaatg
    30241 gaagattgat ttcacccgcg agccgttcgc cagcaacggg ctgtttgcca ttaccggccc
    30301 aaccggtgcg gggaaaacca ccctgctgga cgccatttgt ctggcgctgt atcacgaaac
    30361 tccgcgtctc tctaacgttt cacaatcgca aaatgatctc atgacccgcg ataccgctga
    30421 atgtctggcg gaggtggagt ttgaagtgaa aggtgaagcg tatcgcgcgt tctggagcca
    30481 gaatcgggcg cgtaaccagc cggacggtaa tttgcaggtg cctcgcgtag agctggcgcg
    30541 ctgcgccgac ggcaaaattc tcgccgacaa agtgaaagat aagctggaac tgacagcgac
    30601 gttaaccggg ctggattacg ggcgcttcac ccgttcgatg ctgctttcgc aggggcaatt
    30661 tgctgccttc ctgaatgcca aacccaaaga acgcgcggaa ttgctcgagg agttaaccgg
    30721 cactgaaatc tacgggcaaa tctcggcgat ggtttttgag cagcacaaat cggcccgcac
    30781 tgagctggag aagctgcaag cgcaggccag cggcgtcacg ttgctcacgc cggaacaagt
    30841 gcaatcgctg acagcgagtt tgcaggtact tactgacgaa gaaaaacagt taattaccgc
    30901 gcagcagcaa gaacaacaat cgctaaactg gttaacgcgt caggacgaat tgcagcaaga
    30961 agccagccgc cgtcaacagg ccttgcaaca ggcgttagcc gaagaagaaa aagcgcaacc
    31021 tcaactggcg gcgcttagtc tggcacaacc ggcacgaaat cttcgtccac actgggaacg
    31081 catcgcagaa cacagcgcgg cgctggcgca tattcgccag cagattgaag aagtaaatac
    31141 tcgcttacag agcacaatgg cgcttcgcgc gagcattcgc caccacgcgg cgaagcagtc
    31201 agcagaatta cagcagcagc aacaaagcct gaatacctgg ttacaggaac acgaccgctt
    31261 ccgtcagtgg aacaacgaac tggcgggttg gcgtgcgcag ttctcccaac aaaccagcga
    31321 tcgcgagcat ctgcggcaat ggcagcaaca gttaacccat gctgagcaaa aacttaatgc
    31381 gcttgcggcg atcacgttga cgttaaccgc cgatgaagtt gctaccgccc tggcgcaaca
    31441 tgccgagcaa cgcccactgc gtcagcgcct ggtcgcgctg catgggcaga ttgttcccca
    31501 acaaaaacgt ctggcgcagt taatggtcac tatccagaat gtcactctgg aacagacgca
    31561 acgtaatgtc gcacttaacg aaatgcgcca gcgttataaa gaaaagacgc agcaacttgc
    31621 cgatgtgaaa accatttgcg agcaggaagc gcgcatcaaa acgctggaag cccagcgtgc
    31681 acagttacag gcgggtcagc cttgtccact ttgtggttcc accagccacc cggcggtcga
    31741 ggcgtatcag gcgctggagc ctggtgttaa tcagtctcga ttactggcgc tggaaaacga
    31801 agttaaaaag ctcggcgaag aaggtgcggc gctgcgcggg caactggatg cattaacgaa
    31861 gcagcttcag cgcgatgaaa acgaagcgca aagcctccga caagatgagc aagcacttac
    31921 tcaacaatgg caagccgtca cggccagcct caatatcacc ttgcagccac aggacgatat
    31981 tcaaccgtgg ctggatgcac aagatgagca cgaacgccag ctgcggttac tcagccaacg
    32041 acatgaatta caagggcaga ttgccgcgca taatcagcaa attatccagt atcaacagca
    32101 aattgaacaa cgtcagcagc agctcttaac ggcattggcg ggttatgcac tgacattgcc
    32161 acaggaagat gaagaagaga gctggttggc gacacgtcag caagaagcgc agagctggca
    32221 gcaacgccag aacgaattaa ccgcgctgca aaaccgtatt cagcagctga caccgattct
    32281 ggaaacgttg ccgcaaagtg atgatctccc gcacagcgaa gaaactgtgg cgctggataa
    32341 ctggcggcag gttcatgaac aatgtctcgc attacacagc cagcagcaga cgttacagca
    32401 acaggatgtt ctggcggcgc aaagtctgca aaaagcccaa gcgcagtttg acaccgcgct
    32461 acaggccagc gtctttgacg atcagcaggc gttccttgcg gcgctaatgg atgaacaaac
    32521 actaacgcag ctggaacagc tcaagcagaa tctggaaaac cagcgccgtc aggcgcaaac
    32581 gctggtcact cagacagcag aaacgctggc acagcatcaa caacaccgac ctgacgggtt
    32641 ggctctcact gtgacggtgt agcagattca gcaagagtta gcgcaaactc accaaaagtt
    32701 gcgtgaaaac accacgagtc aaggcgagat tcgccagcag ctgaagcagg atgcagataa
    32761 ccgtcagcaa caacaaacct taatgcagca aattgctcaa atgacgcagc aggttgagga
    32821 ctggggatat ctgaattcgc taataggttc caaagagggc gataaattcc gcaagtttgc
    32881 ccaggggctg acgctggata atttagtcca tctcgctaat cagcaactta cccggctgca
    32941 cgtgcgctat ctgttacagc gcaaagccag cgaggcgctg gaagtcgagg ttgttgatac
    33001 ctggcaggca gatgcggtac gcgatacccg taccctttcc ggcggcgaaa gtttcctcgt
    33061 tagtctggcg ctggcgctgg cgctttcgga tctggtcagc cataaaacac gtattgactc
    33121 gctgttcctt gatgaaggtt ttggcacgct ggatagcgaa acgctggata ccgcccttga
    33181 tgcgctggat gccctgaacg ccagtggcaa aaccatcggt gtgattagcc acgtagaagc
    33241 gatgaaagag cgtattccgg tgcagatcaa agtgaaaaag atcaacggcc tgggctacag
    33301 caaactggaa agtacgtttg cagtgaaata actattcagc aggataatga atacagaggg
    33361 gtgaattatc tcttggcctt gctggtcgtt atcctgcaag ctatcacttt attggctacg
    33421 gtgattggta gccgttctgg tggttgtgat ggtggtatga aaaaagtcat tttatctttg
    33481 gctctgggca cgtttggttt ggggatggcc gaatttggca ttatgagcgt gctcacggag
    33541 ctggcgcata acgtaggaat ttcgattcct gctgccgggc atatgatctc gtattatgca
    33601 ctggtggtgg tggtcggtgc gccaatcatc gcactctttt ccagccgcta ctcactcaaa
    33661 catatcttgt tgtttctggt ggcgttgtgc gtcattggca acgccatgtt cacgctctct
    33721 tcgtcttacc tgatgctcgc cattggtcgg ctggtatccg gctttccgca tggcgcattt
    33781 tttggcgtcg gagcgatcgt gttatcaaaa attatcaaac ccggaaaagt caccgccgcc
    33841 gtggcgggga tggtttccgg gatgacagtc gccaatttgc tgggcattcc gctgggaacg
    33901 tatttaagtc aggaatgctg gcgttacacc tttttattga tcgctgtttt taatattgcg
    33961 gtgatggcat cggtctattt ttgggtgcca gatattcgcg acgaggcgaa aggaaatctg
    34021 cgcgaacaat ttcacttttt gcgcagcccg gccccgtggt taattttcgc cgccacgatg
    34081 tttggcaacg caggtgtgtt tgcctggttc agctacgtaa agccatacat gatgtttatt
    34141 tccggttttt cggaaacggc gatgaccttt attatgatgt tagttgggct agggatggtg
    34201 ctgggaaata tgctaagtgg caggatttca ggacgttatt caccactgcg cattgcagca
    34261 gtgactgact ttatcattgt actggcactg ctgatgctct ttttctgcgg cggcatgaaa
    34321 acaacgtcgc ttatttttgc ttttatttgt tgcgcgggat tatttgccct ttcagcacct
    34381 ctgcaaatat tgttactgca aaatgccaaa ggaggagagt tattaggtgc cgcaggtggg
    34441 caaatagcgt ttaacctcgg tagcgccgtc ggcgcatatt gcggaggtat gatgctgacg
    34501 ctggggctgg catataatta cgtggcgctg cctgccgccc tgctttcgtt tgctgcgatg
    34561 tcatcgttgc tgctgtatgg tcgctataag cgccagcaag cggcggatag tccggtgctg
    34621 gcgaaaccac tggggtagat tactgtcacg gtgcgcgttg cttattgccg gatgcggcgt
    34681 gaacgcctta tccgccctac gcggttctgg cacattttgt aggcctgata agacgcggca
    34741 agcgtcgcat caggcatgat gcgccaattg cctacgtttt ttactcttgt ggccataacc
    34801 acgcagcgcc gcgtacgccg ctggaatcac cgtgcttcgc cttactcacc ggcgtttcac
    34861 attcgccgcc gaagacaaat tgtttaatca actgcccaac cgtttgatat aaacggtcta
    34921 cattgctcat cccgcccccc aggacaatca catccggatc gagaatattc acgacatgtg
    34981 ccagcgattt tgccagccgc agctcgtagc gacgcaatgc cagttccgct accggatcgc
    35041 tttcttcaac caggcggata atttcactgc ctttcagcgc atgtccgctc aaacgacgat
    35101 aatccgtcgc gaatcccgtg cccgaaataa aggtttcaat acaaccttgt ttaccgcaat
    35161 aacaagggac ttcctcgcga taacgcagtt cgtcttcgtc catccacggt agcggattgt
    35221 gtccccactc acctgccgtg ccattgccgc cgatatgcgt ccgcccattg aatgccacgc
    35281 ccgcgccgca tcccgtgccg ataatcacgg caaataccgt ctgcgctccc gctgccgcgc
    35341 catctactgc ttctgaaacc gccagacagt tggcatcatt tgccagccgc acttcccgct
    35401 gcaacctcgc gcttaagtct ttatcgaatg gctgaccgtt gagccaggtt gaattgacat
    35461 tcttcaccac accggtgtag ggcgaaatcg aacctggaat gcccatacct accgttccgc
    35521 gctgccctgt cgcctgctcc gccatatcaa ccaacgtggc gatcgtttca atagtctgcc
    35581 ggtaatcatc acgcggcgtg ggcagacgat ggcggtacaa ctgctcccct gcatcgccca
    35641 gtgcaatcac ttcagttttg gtgccgccta aatcgatacc tatacgcact gtactctcct
    35701 tatttttttc aatatcaata gcgtagagac ggacaaccgg attggcaatg caaggccgcc
    35761 gacaattcgt tatcatgccc gctaaattta acgacaaggc cgtggaaatt atcatgctgt
    35821 ggttcaaaaa tttaatggtt taccgtctta gccgcgagat ttcgctgcgt gcagaagaga
    35881 tggaaaaaca gctagcctcg atggcattta ccccatgcgg cagccaggac atggcgaaga
    35941 tgggctgggt tcctccgatg ggatcgcaca gcgatgcgtt aacgcacgtt gccaatggtc
    36001 aaattgttat ctgcgcgcgc aaagaagaaa aaatcctccc gtctccggtg attaaacagg
    36061 cgctggaagc gaaaatcgcc aaactggaag cggaacaggc gcgtaagctg aagaaaaccg
    36121 aaaaagattc gctgaaagac gaagtgctgc actctctgct gccgcgtgct ttcagccgtt
    36181 ttagccagac aatgatgtgg ctcgacacgg ttaacggttt gattatggtg gactgcgcca
    36241 gtgccaaaaa agcggaagat acgctggcat tactgcgtaa aagcctgggg tcgttaccgg
    36301 ttgtaccatt gagcatggaa aacccgattg aactgacgct gaccgaatgg gttcgctccg
    36361 gtagtgcggc acagggcttc cagctgcttg atgaagccga gctgaaatcg ttgctggaag
    36421 atggcggcgt gatccgcgcg aagaaacaag atctgaccag cgaagagatc accaatcaca
    36481 ttgaagccgg aaaagtggtg actaaactgg cgctcgactg gcagcagcgc attcagtttg
    36541 tgatgtgcga cgatggttcg ctcaagcgtc ttaagttctg cgacgagctg cgcgatcaaa
    36601 acgaagatat cgaccgtgaa gatttcgccc agcgttttga tgccgatttc atcctgatga
    36661 ctggtgaact ggcagcgtta attcaaaacc tgattgaagg attaggtggc gaagcacaac
    36721 gttaattgct gattttcctt taatgccgga tgcgacgcct gccgcgtctt atccggcgta
    36781 cgaagccaca ccaggcatat aattattcgc tacggcgagc aataattttt agcgcagcaa
    36841 tattatgcgt tttacgccgt aactgatcta atcgcctcat ccctattcgc ctgtcagcag
    36901 cacgcagaat attcactaac ccccatttat cgctggtaaa ttgttttgtt aacagctccg
    36961 cttttggggt atcaatatat tcacgcaata aatcagaaat tgaacaaata tccgtcaggt
    37021 aaggatacca gcaagtttca ccgtgaaaat tacgcactcc agcgttacct gcaataaagt
    37081 ctttcgggta atcccagatc acgtttttat ccaacgtgat ccaataacgc ggtaaatcgg
    37141 tacttccacc attttggctg cgcatgggat aacgggtaca gtgaatttgc aggttgatcg
    37201 ttggcgtcag taagtcatag atctcccgct gtaacttgct ccatggacgt tgtgtcattg
    37261 tttttcctca agccggtgac gcgcttcact gcgaattttc cgcgttgccc cggttttcac
    37321 taaatcacgt aactgcgaaa gatcgggttg ttcgacaaat aagagtaatg atggatcctc
    37381 ttttaaccac gccgttttca catcggcagc cagctgtgga ttattaatag ccagcgaccg
    37441 atcctgaggc tcagtcaatg tcgttaacaa cgacgccggg gtatgtgcgt tgcgtaatgc
    37501 cgtttggcgc aaatgatcat tgcgactttg cagcaatgca cgccaccatt catcatctga
    37561 tttatctttt atcgtagcca gttccgcaaa aaatacctgg agatcttcac tgatatttat
    37621 atcaaccttg atgaattatg aatatatcgg aaattgaaaa aatcgcagca aaggaaatat
    37681 tattatgggt taaagagagg atatttcaga aaaatgccct cccgttcaag gggaaggcga
    37741 ggaattacag atagcggcac agataagagg taggttcggc aacttgaaga tgaaactcac
    37801 tgtgaccggg aacattaaac accgaaccgg cttcatacac ttgccagtcg gtcgcgtcag
    37861 gcagtaacac attcagcgcg ccactgatta ccgtcatctc ttccggctca gcggtgctga
    37921 aggtgtattc gccttcaacc ataacaccca cgctggcgcg accagtgctg ctgctggaaa
    37981 agccgatcga tttcactttg ccggaaaagt actcattact ttgaagcata aactggccct
    38041 gtctggtagt tgaacatcta cttcactata ggggccagag gcgctggctg tcacgcaaaa
    38101 ttacaccagt aattccgcag ccagccgtgc aatcaatacg ttagagagca agacaggaac
    38161 atcgagctgt ttttgcagta aatcgcgatg acgttggtga aatcctaaac aatccagcat
    38221 gatgacatca gccccttttg ccagtaattc tttcccagca tcaatgattt tttgttctga
    38281 atcatgaatg gggttaccca atgaaaatac cggcgatttc tgcaaaattt gccatttttg
    38341 cgcctgaacg ggcagcatct cctcaaccgg aacgataacc cccacctgat gatcttcaac
    38401 aatagaggaa accagtggag gcaatattcg cgacggctca agaaagatcg tattacgcgc
    38461 agtcatacta ctaatgtttg ctgtactcat taataaaatg acgtcataac cctgattatc
    38521 gagcacttca accacacctt gcagatctcg ctccacttta cgacgcgaaa catgggccag
    38581 ctggttgtca tttaataatg tgagaatggt gtcttcgcct gcttctggcg cgtactccgc
    38641 catcacttct tcacgactta acttccccag caggctatga tgggaaatat tatcttcgtc
    38701 aatgtattcc gtcaggagcg gcaaaacctc ctgcataggt acaatgccga tggtcaggat
    38761 cgccaacgac gcactcattg ttcatccacc ttacttttct ttcgtcgtta attaccgggc
    38821 aagtgtgaag caccactgct gacacttact tgtgctacaa atgacaaaaa gcgtagcagc
    38881 agacgtgcac ggcataatgt aaagattcca aatgattcca gtaatggaat gttattgttt
    38941 aatattctaa ttattagaaa aacataaatt atgaaaaatg tgacgcagat cccttgtagt
    39001 ggcactcaga atcccttccg agcagtctga tgcggtcact ctttgtcagg gtcctcatag
    39061 cgttttttcg catccatcgc ttcctgagcc gtcggatgtt cactgatcaa cgagtctggt
    39121 ttaggatgat cggctctgag ttggtaccag gttaccgtct gtcctggctt tcctttttca
    39181 atcgtcacta tatatgcttc acgaggataa ggcggtttcg ttggcatcgt tcttccttat
    39241 ttcacgggat gaacgttaag tataggcgct ctaaaatcaa caattgatcg tctgtgccag
    39301 ggcgctacga atttcagaaa tcacctggct gggttcgttt gttgcgtcga tgataatatg
    39361 cgcaacttcg cgatatagcg catcgcgttc ttccagcact tcctgaactt cttcgctcag
    39421 cggttttccc gttaaggttg gccgtaaatc ttcttccggt gcagcttgca gtcggttaac
    39481 caggactgat actggcgcac acaaataaac cacgatcccg ttattttgca tgaagtgacg
    39541 attaaattcc gtcagaataa tgccgccgcc tgtagcgata acggtggatg ccgcagttac
    39601 cgcttccagc gccgccgttt ctctggcgcg aaatcccgcc cactcttccc tttcgacgat
    39661 ctccgcgacc gtcatattga gctgtgattg caaccactga tcggtatcga caaaccgacg
    39721 gttaagcgaa tcggcaaggg ccattccgac cgttgtttta ccacagcccc gaggcccgat
    39781 cagaaaaaga ggttgtgtca tcgtgggttt tccccaatag gtcgcaatgc ggcgaaagcc
    39841 ggtgtcatga gaatagcgat cataccatca aactagtaca atttcgattg taaagaaaaa
    39901 attccactta aagtaaaaat ctcaatacat cccttactat accaataaat attcaagaat
    39961 gaagtgtaaa taataaatta catttagcca cgactacgtt gcacttccag ccaccacttc
    40021 tccagctccg cggcaaaggc ctggcggtca cgttgtgaaa ggctatctgg tccgccggtc
    40081 tggatcccac tggcacgtaa ggtatccata aaatcgcgca tcgtcaggcg ctcacgaatg
    40141 gtcgctggcg tgtaacgttc gccgcgcgaa ttaagcgccg ctgcgccttt ctcgatggct
    40201 tcagcagcca aaggtatatc tgcggtgatc accaaatcgc ccgcttcaca ctgccggaca
    40261 atttcgttat cggcaacatc gaaacctgcc gcgacgcgca gcgtacgaat aaatcgcgat
    40321 ggcggcacgc gtaaactctg gtttgctacc agtaccagcg gcatctgcat acgttccgcc
    40381 gcgcgataca aaatctcttt aattacattg ggacacgcgt cggcatccac ccaaattgtc
    40441 ataaagtcat cctttgttag gtaatcctct attgtgtcgc gcttttgcct tccggcatag
    40501 ttctgtttat gcttctgcca gcgattatca aaacaatgaa tttcacggca ggagtgaggc
    40561 aatggaaaag aaaatcggtt ttattggctg cggcaatatg ggaaaagcca ttctcggcgg
    40621 tctgattgcc agcggtcagg tgcttccagg gcaaatctgg gtatacaccc cctccccgga
    40681 taaagtcgcc gccctgcatg accagttcgg catcaacgcc gcagaatcgg cgcaagaagt
    40741 ggcgcaaatc gccgacatca tttttgctgc cgttaaacct ggcatcatga ttaaagtgct
    40801 tagcgaaatc acctccagcc tgaataaaga ctctctggtc gtttctattg ctgcaggtgt
    40861 cacgctcgac cagcttgccc gcgcgctggg ccatgaccgg aaaattatcc gcgccatgcc
    40921 gaacactccc gcgctggtta atgccgggat gacctccgta acgccaaacg cgctggtaac
    40981 cccagaagat accgctgatg tgctgaatat tttccgctgc tttggcgaag cggaagtaat
    41041 tgctgagccg atgatccacc cggtggtcgg tgtgagcggt tcttcgccag cctacgtatt
    41101 tatgtttatc gaagcgatgg ccgacgccgc cgtgctgggc gggatgccac gcgcccaggc
    41161 gtataaattt gccgctcagg cggtaatggg ttccgcaaaa atggtgctgg aaacgggaga
    41221 acatccgggg gcactgaaag atatggtctg ctcaccggga ggcaccacca ttgaagcggt
    41281 acgcgtactg gaagagaaag gcttccgtgc cgcagtgatc gaagcgatga cgaagtgtat
    41341 ggaaaaatca gaaaaactca gcaaatcctg atgactttcg ccggacgtca ggccgccact
    41401 tcggtgcggt tacgtccggc tttctttgct ttgtaaagcg ccaaatctgc cgatttcaac
    41461 cactcacgat agtgactcat ttgtgggttc agcggcgcaa cccccacact aatccgtaaa
    41521 gttacctgtg gcgtattcgg caagcgtaac gtgtttagcc cttcacgcac ccgtaacatg
    41581 gcggtaatgg cgctctcagc tggcgtaccg gacatgatta ctgcaaactc atcgccgcca
    41641 aaccgaccaa tcacatcgct accgcgcagg gtaatttgta actgtcgggt aagcgccaca
    41701 atcgcttcat cgcccacatc atgaccccag gtatcgttga tgctcttgaa atggtcgata
    41761 tcgataatca gtaacgttgc atcgcgatta tgccgccgac agttatcaaa ttcattgcgt
    41821 aacatagttt cccaatgacg tcggttatac acgcccgtca tgccgtcgcg agtactcatg
    41881 acctgcaacc tgcgtttatg ttccgccagt ttggttgccg tctggtagct gacccagcca
    41941 aacatcagag gataaacgac aataatggga agggagagcc accattccag cggagcacta
    42001 ttgaacgaca cggtaatgcc cgtaagctcg agggtgacaa ggcaggaaac caccatcaac
    42061 accagacccg cgacaaacag acgggggccg cctgccccca tcaaattcag acacagaatc
    42121 atcaacatag cggtggaagg cagcacgttt acgcccatta cgcctaccca cattcccgct
    42181 aatactgcat cggtttttaa gttgtaaatt tcccggctaa gcggatcgac ggccctgctc
    42241 gctatctgcc aggctaaatg cggccagacg aacgcccagc cgaccaacac cagccaccac
    42301 cagcccggcg gcgggtgcga aaccagcgtt gaagcaatcg gtaagaacat gccagccagg
    42361 ccaaccgcac ggggtagtct gacgcgacgg gcgaagcgca gcccggaccg ctgatgttcg
    42421 ttttgaggag ttaaaggagg ttcctcccca tgcgccgccg cttttttgaa aaagttttca
    42481 tcattcatta tttttgggaa cattcagaaa caattttccc aaattataga gacggggcta
    42541 aaggcagaag tatgaaattt cgggtgagcc actttcgtag ctcacccgtt agcacta
//
LOCUS       DAZSIO010000016        41158 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZSIO010000016 DAZSIO010000000
VERSION     DAZSIO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41158
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.016"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..209)
                     /locus_tag="ACHX5T_000753"
     CDS             complement(<1..209)
                     /locus_tag="ACHX5T_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR8466070.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHX5T_000754"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHX5T_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR8466071.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHX5T_000755"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHX5T_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR8466072.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHX5T_000756"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHX5T_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR8466073.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHX5T_000757"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHX5T_000757"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR8466074.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHX5T_000758"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHX5T_000758"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR8466075.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHX5T_000759"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHX5T_000759"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR8466076.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="ACHX5T_000760"
     CDS             complement(4598..5320)
                     /locus_tag="ACHX5T_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8466077.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHX5T_000761"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHX5T_000761"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR8466078.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="ACHX5T_000762"
     CDS             6520..6738
                     /locus_tag="ACHX5T_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR8466079.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHX5T_000763"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHX5T_000763"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR8466080.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="ACHX5T_000764"
     CDS             complement(7472..7672)
                     /locus_tag="ACHX5T_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR8466081.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACHX5T_000765"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACHX5T_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACHX5T_000766"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACHX5T_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR8466082.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="ACHX5T_000767"
     CDS             10101..12014
                     /locus_tag="ACHX5T_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8466083.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="ACHX5T_000768"
     CDS             12014..13036
                     /locus_tag="ACHX5T_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR8466084.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="ACHX5T_000769"
     CDS             13030..13248
                     /locus_tag="ACHX5T_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR8466085.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="ACHX5T_000770"
     CDS             13302..14171
                     /locus_tag="ACHX5T_000770"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR8466086.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="ACHX5T_000771"
     CDS             complement(14226..14630)
                     /locus_tag="ACHX5T_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR8466087.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="ACHX5T_000772"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="ACHX5T_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR8466088.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="ACHX5T_000773"
     CDS             15615..17705
                     /locus_tag="ACHX5T_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR8466089.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD"
                     /locus_tag="ACHX5T_000774"
                     /gene_synonym="dapC"
     CDS             complement(17772..18992)
                     /gene="argD"
                     /locus_tag="ACHX5T_000774"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR8466090.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="ACHX5T_000775"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="ACHX5T_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR8466091.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="ACHX5T_000776"
     CDS             complement(19673..20275)
                     /locus_tag="ACHX5T_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR8466092.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="ACHX5T_000777"
     CDS             complement(20265..20432)
                     /locus_tag="ACHX5T_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR8466093.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="ACHX5T_000778"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="ACHX5T_000778"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR8466094.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22569
                     /gene="tsgA"
                     /locus_tag="ACHX5T_000779"
                     /pseudo
     CDS             21389..22569
                     /gene="tsgA"
                     /locus_tag="ACHX5T_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
     gene            22831..25374
                     /gene="nirB"
                     /locus_tag="ACHX5T_000780"
     CDS             22831..25374
                     /gene="nirB"
                     /locus_tag="ACHX5T_000780"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR8466095.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25371..25697
                     /gene="nirD"
                     /locus_tag="ACHX5T_000781"
     CDS             25371..25697
                     /gene="nirD"
                     /locus_tag="ACHX5T_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR8466096.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25823..26629
                     /gene="nirC"
                     /locus_tag="ACHX5T_000782"
     CDS             25823..26629
                     /gene="nirC"
                     /locus_tag="ACHX5T_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR8466097.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26648..28021
                     /gene="cysG"
                     /locus_tag="ACHX5T_000783"
     CDS             26648..28021
                     /gene="cysG"
                     /locus_tag="ACHX5T_000783"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR8466098.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28268..28435
                     /locus_tag="ACHX5T_000784"
     CDS             28268..28435
                     /locus_tag="ACHX5T_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR8466099.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28730..30067
                     /gene="frlA"
                     /locus_tag="ACHX5T_000785"
                     /pseudo
     CDS             28730..30067
                     /gene="frlA"
                     /locus_tag="ACHX5T_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30088..31110
                     /gene="frlB"
                     /locus_tag="ACHX5T_000786"
     CDS             30088..31110
                     /gene="frlB"
                     /locus_tag="ACHX5T_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR8466100.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31160..31990
                     /gene="frlC"
                     /locus_tag="ACHX5T_000787"
     CDS             31160..31990
                     /gene="frlC"
                     /locus_tag="ACHX5T_000787"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFR8466101.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31987..32772
                     /gene="frlD"
                     /locus_tag="ACHX5T_000788"
     CDS             31987..32772
                     /gene="frlD"
                     /locus_tag="ACHX5T_000788"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR8466102.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32872..33603
                     /gene="frlR"
                     /locus_tag="ACHX5T_000789"
                     /gene_synonym="yhfR"
     CDS             32872..33603
                     /gene="frlR"
                     /locus_tag="ACHX5T_000789"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR8466103.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33763..34848)
                     /locus_tag="ACHX5T_000790"
     CDS             complement(33763..34848)
                     /locus_tag="ACHX5T_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR8466104.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34860..36164)
                     /locus_tag="ACHX5T_000791"
     CDS             complement(34860..36164)
                     /locus_tag="ACHX5T_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR8466105.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36176..36529)
                     /locus_tag="ACHX5T_000792"
     CDS             complement(36176..36529)
                     /locus_tag="ACHX5T_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR8466106.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36540..37418)
                     /locus_tag="ACHX5T_000793"
     CDS             complement(36540..37418)
                     /locus_tag="ACHX5T_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR8466107.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37415..38641)
                     /locus_tag="ACHX5T_000794"
     CDS             complement(37415..38641)
                     /locus_tag="ACHX5T_000794"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR8466108.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38642..39805)
                     /locus_tag="ACHX5T_000795"
     CDS             complement(38642..39805)
                     /locus_tag="ACHX5T_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFR8466109.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39889..40251)
                     /locus_tag="ACHX5T_000796"
     CDS             complement(39889..40251)
                     /locus_tag="ACHX5T_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR8466110.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40268..>41158)
                     /gene="yhfZ"
                     /locus_tag="ACHX5T_000797"
     CDS             complement(40268..>41158)
                     /gene="yhfZ"
                     /locus_tag="ACHX5T_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR8466111.1"
                     /translation="IKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVGLTQAA
                     LKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEGLASGL
                     KAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALELGPHT
                     YVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESLQRIVK
                     GDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLLRAVVD
                     KHALLAHQQRVVSGEQEPSY"
BASE COUNT         9924 a        11176 c        10340 g         9718 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaaga gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgctaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa gaaaagtggg ggatcggcgt actgtttctc
    22021 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22081 tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg
    22141 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22201 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22261 acaccagtac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22321 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22381 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcacgatt
    22441 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22501 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22561 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22621 cgctacgtgc atctatttct ataaacccgt tcattttgtc cattttttgc acaaacatga
    22681 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22741 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22801 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22861 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22921 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctca
    22981 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23041 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23101 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcaaccggt
    23161 tcctacccgt ggatcccacc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23221 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23281 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23341 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23401 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23461 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23521 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23581 acccagtgtg gtctggacgt tgctccgcgt ggggggattg tcattaatga ttcctgccag
    23641 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23701 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctgggtagc
    23761 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23821 ggtggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctatgt ttacctcgac
    23881 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    23941 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaacgct
    24001 atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg tagcggtaag
    24061 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24121 actaaaggtg atctgattgc tgccatcaac aaaggctgcc acacagttgc ggcgctgaaa
    24181 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24241 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24301 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24361 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg
    24421 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24481 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgt
    24541 tctccgggcg gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat cgcgcgtgaa
    24601 tttaatctct acaccaaaat caccggctcc cagcgcctgg cgatgtttgg cgcacagaaa
    24661 gacgatctgc cggaaatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24721 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggt
    24781 gttggcgata gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24841 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24901 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    24961 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25021 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25081 gcgccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25141 gacaaactgg ggctgaacgc tcatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25201 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25261 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25321 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25381 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcactgttag
    25441 gtgacaagca ggtagcgatt ttccgcccgt atcacagcga ccaggtgttt gcgatcagca
    25501 acattgatcc gttcttcgag tccagcgtgc tatcacgcgg gctgattgcg gaacaccagg
    25561 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25621 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25681 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25741 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25801 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaatgc
    25861 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25921 ggcgggcgca tatgtgggtc ttgggatcat cctgattttc acgctcggta atttgctcga
    25981 cccgtccgta cgtccgctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26041 tatcgccggt tctgaactgt tcaccggaca caccatgttc ctcacctttg gggtaaaagc
    26101 gggcagcatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26161 ggtcggttcc gtcttcgttg ccatgctcta tagctggggc ggcggtagcc tgctgccggt
    26221 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26281 gttcttcaaa ggtgcattgt gtaactggct ggtttgcctg gcaatctgga tggcgctgcg
    26341 cactgaaggg gcggcgaaat ttatcgctat ctggtggtgt ctgctggcat ttatcgcgtc
    26401 cggctacgag cactctatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26461 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26521 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26581 tcgtccggtt gcggacaaat ttaatcaaac tgaaacagct gccggttaat tactaagggg
    26641 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26701 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26761 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26821 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26881 agcgacggat gatgacacgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    26941 cttctgtaac gtagttgatg cgccgaaagc cgccagcttt attatgccgt cgattattga
    27001 ccgctcaccg ctaatggtag cggtctcctc tggcggcacc tctccggttc tggcgcgcct
    27061 gttgcgcgaa aaactcgaat cactgctgcc gttgcatctg ggccaggtgg cgaaatatgc
    27121 cgggcaatta cgcgggcgag tgaaacaaca gttcgccacg atgagtgagc gtcgccgttt
    27181 ctgggagaaa ttgttcgtta acgaccgcct ggcgcagtcg ctggcaaaca acgatcagaa
    27241 agccattact gaaacgaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27301 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acgctgaaag gactgcaaca
    27361 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27421 ggtacgccgc gatgctgatc gcgttttcgt cggcaaacgc gcgggatacc actgtgtacc
    27481 gcaggaagag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg
    27541 gctgaaaggt ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27601 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctctgc
    27661 ctattcgggt attccactca cgcatcgcga ttatgcccag agcgtacgct taattaccgg
    27721 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27781 ggtgttctat atggggttga atcaggccgc gactattcag caaaagctga ttgaacacgg
    27841 tatgcctggc gaaatgccgg tggcaattgt cgaaaacgga acggcagtca cgcagcgcgt
    27901 gattgacggt acgctcacgc aactcggtga acttgctcag caaatgaaca gtccatcgct
    27961 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaatcatta
    28021 atttaacccg ggccagagaa ttctggcctt cttaatgact ctttttatat attcagcaaa
    28081 taaaacataa ccccttaata aatattatct gctaatgggt tatattcacc gttacagcga
    28141 tttacaaaat taagtcattc gctcactgac gccaattaaa tattcctgta tcgttcttaa
    28201 cgagcagtct gcttatattc atataatcaa tgaatattaa ttaatatata atacataggg
    28261 aatgtaaatg aacaaattta ttaaagttgc actggtaggt gcagtactgg ctacgttaac
    28321 tgcatgtact ggtcatattg aaaaccgtga taagaactgc tcttacgact acctgctgca
    28381 cccggcaatt tctatttcta aaatcattgg cggttgcggt cctactgcac agtaagtctc
    28441 tgacagaaat cggctaacac cggtttaatt gcccgacgcc ctctggattt tccgagggcg
    28501 tatttgtttc tatgtccttc ttgcccccgc ccgtaaaaat attttcactt taaattcaat
    28561 tttataacta catccattag ccacagcgcg atcctgtgcg aaattttgtg atcttcctcc
    28621 acatcacata acataatatg ttgttatatt catcatgcat tgtcatgtta ccttttaaat
    28681 ggctgcaaac tctcccctac aacgtattgg acaagaaaaa ggtatcgcta tgggaagcca
    28741 ggaactccaa cgcaagctcg gattttgggc cgttcttgca atcgccgtcg ggacaaccgt
    28801 cggctccggt atttttgtat ctgtgggtga agtggcaaaa gcagcgggca cgccgtggct
    28861 tacggtgctc gcgtttgtca ttggcgggtt aattgtgatc ccgcaaatgt gcgtctatgc
    28921 ggaactatcc accgcttatc cggaaaatgg cgcagattat gtttatctga aaaatgccgg
    28981 aagccgaccg ctggctttcc tctccggctg ggccagcttc tgggccaacg atgcgccgtc
    29041 attgtcgatt atggcgctgg cgattgtcag caatcttggc tttttaacgc ctatcgatcc
    29101 gttgctcggt aaatttatcg ccgccggatt aattatcgcc tttatgttgc tacacctgcg
    29161 ctccgttgaa ggcggcgcag cgtttcagac gctaattacc atcgccaaaa ttatcccgtt
    29221 cactatcgtc attggccttg ggatcttctg gtttaaagcg gagaattttg ccgcccttac
    29281 caccactgcg attggcgcaa cgggcagctt tatggcgctg ctggcgggga tctctgccac
    29341 cagttggtcg tataccggca tggcctctat ctgttatatg accggcgaaa ttaaaaaccc
    29401 cggaaaaacc atgccacgag cgctgattgg ttcctgtctg ctggttctgg tgctctacac
    29461 cctgctggcg ctggtgattt ccggcctgat gcccttcgac aaactcgcca attctgaaac
    29521 gccgatttcc gacgccctga cctagatccc cgcactcggc agcaccgctg ggctctttgt
    29581 tgccatcacg gcgatgatcg tcattcttgg ttcgctttcc agctgcgtga tgtaccagcc
    29641 gcggctggaa tacgcgatgg cgaaagacaa cctgttcttt aaatgcttcg gccatgcgca
    29701 tccgaaatac aacacgccgg atgtctccat catcctgcaa ggggcgctgg ggatcttctt
    29761 catcttcgtt tccgatctca ccagcctgct gggttatttc accctggtga tgtgtttcaa
    29821 aaataccctc accttcggct ccatcatctg gtgtcgtaaa cgcgacgatt acaaaccgct
    29881 gtggcgtact ccggctttcg ggctgatgac caccctcgcc attgcgtcaa gcctcattct
    29941 ggtcgcctca acctttgtct gggcaccgat tcccggcctt atctgcgccg ccatcgttat
    30001 tgctactggt ctgcctgctt acgccttctg ggcgaagcgt agccgccagc tcaacgcttt
    30061 gtcgtaatgt tatctgaaga aaataaaatg ttggatattg ataaaagcac cgtggacttt
    30121 ctggtcaccg aaaatatggt tcaggaagtg gaaaaagttc tcagccatga cgttccgctg
    30181 gtgcacgcca tcgtggaaga gatggtgaag cgcgacattg atcgtattta tttcgttgcc
    30241 tgcggctcgc cactcaacgc ggcgcaaacg gcgaaacatc tggcggatcg cttttccgat
    30301 cttcaggtct acgccatttc cggctgggag ttctgcgata acaccccgta tcgcctcgac
    30361 gatcgttgcg cggtgattgg cgtttctgac tacggtaaaa cggaagaggt aatcaaagcg
    30421 ctggagctgg gccgggcctg cggagcactc actgcggcgt tcaccaaacg cgcggacagc
    30481 ccgattacct cggcggcgga atttagcatt gattatcagg ccgactgtat ctgggaaatt
    30541 cacctgctgc tctgctacag cgtggtgctg gagatgatca cccgcctcgc gccgaacgcg
    30601 gaaatcggca agatcaaaaa cgatctcaag cagttgccga atgcgctcgg tcatctggta
    30661 cgcacctggg aagaaaaagg ccgccagctt ggtgaactgg ccagccagtg gccgatgatt
    30721 tataccgttg ctgcgggtcc gctgcgtccg ctgggttaca aagaaggcat tgtaacgctg
    30781 atggaattta cctggacgca cggctgcgtg attgagagcg gagagttccg ccatggcccg
    30841 ctggagattg tcgaaccggg cgttccgttc ctgttcctgc tcggcaatga tgaaagtcgc
    30901 cacaccaccg aacgcgccat taactttgtt aaacagcgta ctgacaacgt gatcgtcatc
    30961 gattacgccg aaatttcgca agggctgcac ccgtggctgg caccgttcct gatgttcgtg
    31021 ccaatggagt ggctctgcta ctacctgtct atttacaaag atcacaaccc ggatgaacgc
    31081 cgctattacg gtggtctggt ggaatattaa tccctctccc ggcccggcaa cgggccggat
    31141 ttacgcaagg agttacccga tgaaaacagg tatgtttacc tgcggccacc agcggctgcc
    31201 tattgaacac gcatttcgtg atgcaagcga gctgggttac gacggcatcg aaatttgggg
    31261 cggtcgcccg cacgcgttcg cgccggactt aaaagcgggc ggcatcaaac aaatcaaggc
    31321 gctggcgcag acgtatcaga tgccgattat cggttacacg ccagaaacca acggctatcc
    31381 gtataacatg atgctgggcg atgaacatat gcgtcgtgaa agcctggaca tgatcaagct
    31441 ggcgatagat atggcaaaag agatgaacgc gggttatacg ctgatttctg cggcccacgc
    31501 gggctatctc acgccgccta acgttatctg ggggcggctg gcagagaact taagcgaact
    31561 gtgtgagtac gcggaaaaca tcggcatgga tctgatcctc gaaccgttaa cgccgtatga
    31621 atcgaacgtc gtgtgtaatg ctaatgatgt gcttcatgcg ctggcgctgg tgccttcgcc
    31681 gcgcctgttc agcatggtcg acatttgcgc gccgtatgtc caggcggaac cggtgatgag
    31741 ttatttcgac aaactgggcg ataaattacg tcatctgcat attgtcgaca gcgacggggc
    31801 cagcgacacg cattacattc ctggagaagg caaaatgccg ctgcgggaac tgatgcgcga
    31861 tattattgat cggggctatg agggttactc tacggtggag ctggtgacga tgtatatgaa
    31921 cgagcccaga ctctatgccc gccaggcgct ggaacgcttt cgcgcgctgc tgccggagga
    31981 tgagagatga aaaccctggc gacaatcggc gataactgcg tcgatatcta cccgcaactg
    32041 aataaagcgt tttctggcgg taatgcggtc aatgtggcgg tgtactgcac tcgctacggc
    32101 atacagccgg gatgcattac ctgggtgggt gacgatgact acggcacaaa gctgaagcag
    32161 gatctcgccc gcatgggcgt cgatatcagc catgtccata cgaaacacgg cgttaccgca
    32221 caaactcagg tggaactgca cgacaatgat cgcgtttttg gcgactacac cgaaggcgtg
    32281 atggccgact ttgccctgag tgaagaggat tacgcctggc tggcgcagta tgacattgtg
    32341 cacgcggcaa tctggggaca tgcggaagac gcattcccac agctgcacgc tgcgggcaaa
    32401 cttaccgctt tcgacttctc cgacaagtgg gacagcccgc tctggcagac actggtgccg
    32461 catctcgatt ttgcctttgc ctccgcaccg caagaagacg aagcgctgcg tctgaagatg
    32521 aaagcgattg ttgcccgtgg cgcaggaaca gtgattgtca cgctgggtga aaacggcagc
    32581 attgcctggg atggcgcgca gttctggcgt caggctcctg aaccggtgac ggttatcgac
    32641 accatgggtg ccggagattc gttcattgcc ggattccttt gcggctggtc tgcggggatg
    32701 acattaccgc aggcgatggc gcagggaacg gcgtgcgcgg cgaaaaccat tcagtaccac
    32761 ggtgcctggt aggtataacg ttggcgtgag catcttcacg ccaacgtgct gttacttgcc
    32821 ggaaaacgac cctataatcc gagtaattca ttctttattt cagggtcgat tatgtcagct
    32881 acggaccgct actctcatca actcctctac gctaccgtcc gccagcggct gctggatgat
    32941 atcgcgcagg gggtttacca ggccgggcaa cagatcccta ccgaaaacga gctttgtaca
    33001 caatataacg tcagccgcat taccattcgc aaagccatca gcgacttagt ggcagacggc
    33061 gtactgatcc gctggcaggg aaaaggcacc tttgtacaaa gccagaaagt tgaaaacgcc
    33121 ctgcttactg tcagtggttt taccgatttt ggcgtctcac aaggcaagtc gacgaaagag
    33181 aaagtgatcg aacaggaacg ggtcagcgcc gcgccgtttt gcgaaaagct gaacatcccc
    33241 ggaaacagcg aagtgttcca tctctgccgg gtgatgtatc tcgataaaga gccgctgttt
    33301 attgatagtt catggatccc gctgtcgcgt tatcctgact ttgatgagat ttacgtcgaa
    33361 ggaagctcca cctatcagtt atttcaggag cgttttgaca cgcgagtggt cagcgacaaa
    33421 aagaccatcg atatctttgc cgccacccgc ccgcaggcaa aatggctgaa atgcgaactg
    33481 ggcgaaccgt tgtttcgcat cagcaaaatc gcctttgatc agaatgacaa accggtgcac
    33541 gtctccgaac tcttctgccg cgccaatcgc atcaccttaa cgattgataa taaaagacat
    33601 taaccgtagg ccggataaga tgcgccagca tcgcatccgg cgatgctggc gcactgaatt
    33661 ttacatcccg cacgttcccc tcaccctaac cctctcccca aaggggcgag gggaccgttc
    33721 agtgcggaat ttagcttgat tgccgaacgg tgctatccag cattaaatac tcgcaatact
    33781 ctcacgcaaa atccgcagca ccgtctcttc accgctgcga ttcgggttaa tgcgaatcgc
    33841 acaatgttcg agttgtggat tcgcctggcg aaacgttcct gaaagtcgat agaagagcgg
    33901 cgggatttca tatttcgact ctgcaccgac cgggtaaggc aacgcgccac gcttttgcgc
    33961 ttcttccagc accctggcgg caatcagctg atgaaactcg acaatcaaca ccttcgactg
    34021 cgcattggca atgacggcgc ttttcacttc cgccaccgcg ccgccgttaa gcaaagccag
    34081 caaccgttcg gatactcccg cctgcaccgc atgcatcact ggcgcgaaca ccagaccacg
    34141 caagacttcc agcgcctgtg agccctggat ctggctaccg ccggagtaaa gcgtggcgcg
    34201 aatccgacta ataacatcgg catcgccaac cactgcacca acgccctccg gcccaaacag
    34261 cttgaagcag gagaatgtcg agacattcgc gccgcattca cagccgattc gcgctacctt
    34321 catcaccgca tagttatcat cggttaacgc tggaacacct gccgcgcgca acgttgccag
    34381 cacatctgcc agcacatagc tgtcctgcgg ctgctggcgc gtatgctgca ccagcgcggc
    34441 atcaggttgt tgctcgtcga cgacctgctt cagtgccgac aggtcattga aatcagcagt
    34501 aataagcgtc agccccatct gctcaataat aacccgtgtc gtcgggtaaa caggcgcgtc
    34561 atgcaccaga agacgctgcc ccggtttgag caaagccgcc agcccggcgc gaatcgcgcc
    34621 agtccccgcg ccctgcacca gcgccgcggc ctgtgcgtga aatgcatcag ccagcacctg
    34681 ctccacccgt tgggtaatac gcggttgatt cagacttgga gttaacccta aatcaccgcc
    34741 agcaagaaac tcgctgcctg gaaaatggcg gcaaatagtg tccaccagcg caaactgctt
    34801 ttgctgcgct tcagcaagag tcaggctttg caaaggaaac gtcttcatag gtgccttcct
    34861 taagccggaa cgaacaggcc aagccagtaa agaacattca gcagaatacc ggtgatcatt
    34921 actgccacga ccggtgccgc cattttctgt accggacggc ccagggattc gttgaggaag
    34981 taaatggcaa ccgcgataga gaatccggta taacccgcca tcttaattgc tgcgaaaatc
    35041 gaaccgacca gcagcgccac ttccatcagc atattcatgg cgttacggat gttatccgac
    35101 gcattacgca ccgacgggta gcgtcccagc catttgccga tcgaacgaag cagcaagact
    35161 tccgccgaaa taaccactgc gcctaatacc gctgcaacca tcggattcgg cgagagatag
    35221 cccaccgcat aaacaaaggt aaagcccgca actgcataca cacccgttgc taaggcggtg
    35281 gtggcaatca acggcacaaa ccccagtccg cgcataaatt ctgccagagc cgcctgatta
    35341 atcagcgttt gcgattgttc cggcgtcacg cctgcggaat acgctttctc cagtgtgaag
    35401 atcgaaactt cgctaccagc aaaaatcttc atactggcaa cggcggcaat caatgctccc
    35461 acgatggcga tataaggtaa gtttttgata atccgcgacg tgcgctcttc aaacaccgaa
    35521 agcccgctgg catcgatgtc attttcatca cgatgacgca ggtcgtgagt tatcgcgatc
    35581 cccagcagca tcaccatgcc aataaagatt tcgatggatt cagggttaag atgtgggaaa
    35641 tagcgcacga ctaccacacg ggtcatcaga acaaccacgg cggcgaccag actttgcttc
    35701 cagccaaact ggtagaaaat cgccaccagc gggaacagtg caaaagcaga aaccaccggc
    35761 gagcttaatt ccccgaggct acctaataca tccaccggca gcgcggtcag cagctggtta
    35821 actggcaaca ggcaagtaag gatcaacacg ccccagatag cgccaagacc aaacgccatc
    35881 aggctgttta tcgccagtac gccgaggata tcggtaggaa gaaagagtaa ccatgcgttg
    35941 agcagcccgg ttttcagggt aaaagagatc cccaccgagg ccacaaaacc aatgctcaga
    36001 ccaaaagcga tactccccgc ctcgcgacgg ttcatatagc cttcaatcag ttgcggcagg
    36061 atcgggtgga tgccgtcatg aaaaacagcc gccgagcgat gctccagaag cgatgtcata
    36121 cccgtcaggc acgccaccac gataatctga atatacagat ccatagtcgt gccttttatt
    36181 tcagatggtt aatcaacatc ggaatcgcgt gttcgacgtg ctcaacggaa aggccaaacg
    36241 ccactttccc ttcagcgatc attttggcga tatgctcgtc tttcgcttta atgcctggtt
    36301 tggcaatggt gcaacttttg ttatagccaa tcaccgcgat ggcaatcgac aatgccgcgc
    36361 ccgcaccggt attacacgcg ccaatgtaat aatctagttg cccggatttc accttcattg
    36421 ccgcttccat gtcgttgtga atgaaaactt caaaacagcc aggagccgtc gcttcaatgg
    36481 tttttttaat ctgctcacgc tgtaagcctg caacgccaat ctttttcatg agtctgtcct
    36541 tattggaaaa attgagaggg attttcacgt aacatcacat ccacatcggc ctgactgaat
    36601 cctgactggc gcaattgcgg aataaaggtg gtcaataaat agtcatagcc ataaccacca
    36661 ttggctttta aatgggagcg gcgcgtaata tccatcgaca gcatgacgcg gttcagcaac
    36721 ccacggtcac gtagcgcgtg gagcatcgca atgcgctttt cgtccgggta gtaactgttc
    36781 ttaccaatgg tgtcgaactg cacgtacgcg ccgagatcga tcatcttcaa aatgttgtcg
    36841 aggttgtctt tcagatcgca gtgaccaacg gtgacgcgcg aaagatcaac tccgtgggct
    36901 tgcaacagag ccagttgttc cagccccatg gtgctgaacg acgtatgcgt ggagatcggg
    36961 cgtccggtct ggttatgcgc cagcgcagcg gcaataaata ccttctcttc cagcggcgta
    37021 atctttcctt cgctggagcc gatctccgcg atgatcccgg ctttcagctc cgtgccatcg
    37081 ataccctgtt caatttcatc gaccatctcc tgcgccagtt cctgcacgct gcgggtcacc
    37141 acatgttccg ggaaaaacgc atcctggtaa taaccagtac aggccaccac gttgatcccg
    37201 gtttcgcgca ttacatcgag cataaattgc gcattgcgcc ccatgtaacg gttggtcatc
    37261 tcaatcacat tacgtacacc ccgggtcatc aggtcgttca tctcctggca aatgaacgca
    37321 tactgatcaa ggcggcagtc cacgttgttt ttaaagccgg agagatcaat atgcagatgc
    37381 tcatgggcca gggtgtaacc cgtcggatca aaactcatag ggtgtctcct gcaaaccgga
    37441 gggaggaaag aaaagagcga ccattttgtg gcggtggcgc acggaaaaat tcacacacgg
    37501 tagctcccac atcagaaagc gtggtgcgca caccgagctg cgtggcgacc agcccttgct
    37561 gataaaccag cactggcacc acttcacggg tatggtggct gtgaccaatg gtcggatcgt
    37621 taccgtgatc cgccatcacg accaggcaat catctggctg catcgcctca acaagccggg
    37681 caaggttacg gtcaacgacc tgcaaacgtt cggcataacg tgcgacgtct tctgcatgac
    37741 cagcgaggtc ggtttcctgg atattgatgc aaataaacgc cgtcggatgg gtgttaaatt
    37801 cgtcgagggt gatatccata atccgctggc tatccaccag gttttgccag ctcacaccat
    37861 aaggattgct gacgatatct gccaccttac ccaccagcac ggttggcacg cccgcttcat
    37921 acagtttttg cggcacctgc actttttcat caacaccgta gcccatatgc acgacctgga
    37981 agccgttgtc ataagcgcca gaacgcggcg cattaatacc aataaagcgc ccttctttgc
    38041 tttctgcggc atcgagaata cgctgactgt tggttaacag gccgccaaat gtaatgaccc
    38101 gaccgacctg tacctggtca cgcacgatac gaccaatttt gattgcgtcg tcaaaagaga
    38161 tcacagagag attggcggta atgttataga cttggcctaa atccgcctcg agattatcgc
    38221 caatcgcaac cgcctgattg accgacagaa attgcagatc atcgccacgg cgctccacct
    38281 gccagccagc ggaaactaat gcctgctcaa cacggtcaat cacatcgcga aaaggcatcc
    38341 gcagcggcgg taacgggcgc gtgcctaaaa tttcctgatg ccccataaag gtatcgccac
    38401 cttcatgttg cagctctgcc acgccccagg ttgcggaatc tgacggctgc atatcgcctg
    38461 gcgcataacc caatgtgttg attagcccca gcttctccag cgttggtagc tgcaaatgcg
    38521 gcaactggct caggatgtga ccacaggtat tcgccccggc atcttgcggg cgcaccagcg
    38581 tgacatcttt cattgcccct acgccaaagc tatcaatcac taacaccaca aatcgcgcca
    38641 tttatgcctc cagagggttg ccgaggctgt cataacgacc aactatttct ggttcgccgc
    38701 gctgaatacc ggacaccagc accacatcgc tacgggtgac aaaaatctgc gtgcgaaaac
    38761 agagcaccac tgcactgctc accggatact cgcctgccag cggcagggtg tagtcgatac
    38821 tgctgtcatc cacagttttg agattggttt cagtaatctt ttgattttct ggcgtaaaca
    38881 ccagcgcatg ttgcgcatga ccacgacggt agtaaccgcc gccgtagcag tagctgtcgc
    38941 cacggaaatg atgggagatt tcacttaacc agagcatcgc gatacgttca ggctgatcgc
    39001 cctgctggtt tgccggaata gtaccggtca gcgcatgacc gggttcggca tgagtcgcac
    39061 cgtattccgc cagcaatggc agcgaagtgc agctggtcgc tgaaggtacg ttcagttgct
    39121 caatcgcaat accagatttc gccagttgat cccgtgcttg taccagcgtg tgaagattcg
    39181 gtgttggcaa aactttccca gcagcctcat cccaaagcag gcaagggaaa tgggttagtc
    39241 cggctaaatg cagccccggc agtttcttaa tttcagcaat cacctcgtgc agcgaatgct
    39301 gtacaaaacc gctctcctgc cccggataaa gaaaatcatc gtcgctataa actttgagca
    39361 gcacaggctg gacccgtccg gccttaaccg ctgccgcaga aacttcccgc gctttgtcga
    39421 gagtaaacac ggtgatgatg tcggtgccct gttcaacggc gtcagcaacc tgatgacaag
    39481 ggatttgtac cagatgcccc tgatgcgcca ctggcaaacc agcgcggcgc atgactcgcg
    39541 cctctttgta atccaccgcc acaatgccgc tgtagcctaa tgccagcaat ttttccgcca
    39601 gccacggatt acgaccaaat tgcttggtca tcagatacag ttcaatgccg taaagccgcg
    39661 ccgtctcaat gagccgctta ccgttttcca gtatctgatc cacgtcgatc acccagctgt
    39721 ccggggcgat tttgccctgc tgccacaaac ttagtgcggc ggaaatcagc gccgggttct
    39781 ggcgtttcaa tgcttctaca aacatcttgt tgcctcgttt ctgttccttg aattaaatat
    39841 tcattttttt gaatatttag gttgtgcgac gtggatttgt cgggagcttc aaacctcttc
    39901 gttagcagcc atccataatc cataaagatt ggccagaaaa tagccttctt cacacgggtt
    39961 aacttccaga gcgaattcct tcaacaactc ctgatgcaat tgcacaacgg cttgccagtg
    40021 gctggattgc gccagttccg ccagcaactc gttatccagc ggctctattt cttcaccacg
    40081 gcgactgcgc atcagtgcac tcgccatatg tgtcatcgcc atcgttcctt gctcactgcg
    40141 caccggcaga tggcactctt tttccagtac gttgacgacc tgcatcatgc ctttgcagac
    40201 gtccttatca ataacgcctg cctcgcaaag caggttgagt ctggtttcca tatcagatgc
    40261 ctttcgttta ataactgggt tcctgttccc cactcaccac ccgttgttga tgggcaagca
    40321 gggcgtgttt atctacaaca gcacgcagca attgttgcat tgggtaatca tcgactcgcg
    40381 tcaggaccac ggcttcggtg gcctgtaaaa atcgcggatc gtctgttagc ggcgtcgcct
    40441 ctaatcccag catggtcagt tcgttttccg ccaccacgtt ccagatcacc gcatcgacat
    40501 cgcctttaac aatgcgttgt aaactctcgt gataagagag atcgactcgt tccacatcac
    40561 tatcgccaaa aaagacatcg gtcatgattt tctgatccgc cgaacgacta tcgagaccta
    40621 ctcgcttaac atttccagac tcacctttgc ggcatatcag ttgatgttca ccaacatacg
    40681 tatgcgggcc aagctcgagg gcgatgcaca aattgttttg gctgagataa ctttccgccg
    40741 ccaggcggga aactactgcc atgtcataga caccgttaag taaacactcc acgcgaatat
    40801 ccgcgccgcg catatgcgca tagtaaaaag gaatgccatc aaactgggct ttcaatccgc
    40861 tcgctaaacc ttcgtacaag cgggtatagg gcaaaggcat cgcacatacc acgttgttga
    40921 tatccacatg actcagcaat gctttgttat ccatctcgac aagataactg ccattgcgcc
    40981 cacggcgttc aatccgtatc gctccgcttg attccagcgt tttcaacgcg gcctgcgtca
    41041 ggccaaccga tgaacggcat tcagttgcca gctcatctat ggttttcagt cgattaccgc
    41101 atttttcacc caacaaataa cgggccagtg tcgtgatgac gacgccttct tttttgat
//
LOCUS       DAZSIO010000017        40842 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZSIO010000017 DAZSIO010000000
VERSION     DAZSIO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40842
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.017"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(88..1338)
                     /gene="icd"
                     /locus_tag="ACHX5T_000798"
     CDS             complement(88..1338)
                     /gene="icd"
                     /locus_tag="ACHX5T_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR8466112.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1510..2163
                     /gene="rluE"
                     /locus_tag="ACHX5T_000799"
     CDS             1510..2163
                     /gene="rluE"
                     /locus_tag="ACHX5T_000799"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR8466113.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2173..2634
                     /gene="nudJ"
                     /locus_tag="ACHX5T_000800"
     CDS             2173..2634
                     /gene="nudJ"
                     /locus_tag="ACHX5T_000800"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR8466114.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2688..3794
                     /gene="mnmA"
                     /locus_tag="ACHX5T_000801"
                     /gene_synonym="trmU"
     CDS             2688..3794
                     /gene="mnmA"
                     /locus_tag="ACHX5T_000801"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR8466115.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3830..4471
                     /gene="hflD"
                     /locus_tag="ACHX5T_000802"
     CDS             3830..4471
                     /gene="hflD"
                     /locus_tag="ACHX5T_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR8466116.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4475..5845
                     /gene="purB"
                     /locus_tag="ACHX5T_000803"
     CDS             4475..5845
                     /gene="purB"
                     /locus_tag="ACHX5T_000803"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR8466117.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6014..6685
                     /gene="phoP"
                     /locus_tag="ACHX5T_000804"
     CDS             6014..6685
                     /gene="phoP"
                     /locus_tag="ACHX5T_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR8466118.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6685..8145
                     /gene="phoQ"
                     /locus_tag="ACHX5T_000805"
     CDS             6685..8145
                     /gene="phoQ"
                     /locus_tag="ACHX5T_000805"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR8466119.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8942..9223)
                     /locus_tag="ACHX5T_000806"
     CDS             complement(8942..9223)
                     /locus_tag="ACHX5T_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466120.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9237..10898)
                     /locus_tag="ACHX5T_000807"
     CDS             complement(9237..10898)
                     /locus_tag="ACHX5T_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR8466121.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10882..11208)
                     /locus_tag="ACHX5T_000808"
     CDS             complement(10882..11208)
                     /locus_tag="ACHX5T_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR8466122.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11264..11446)
                     /locus_tag="ACHX5T_000809"
     CDS             complement(11264..11446)
                     /locus_tag="ACHX5T_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466123.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11430..11870)
                     /locus_tag="ACHX5T_000810"
     CDS             complement(11430..11870)
                     /locus_tag="ACHX5T_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR8466124.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11870..12166)
                     /locus_tag="ACHX5T_000811"
     CDS             complement(11870..12166)
                     /locus_tag="ACHX5T_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFR8466125.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12163..12501)
                     /locus_tag="ACHX5T_000812"
     CDS             complement(12163..12501)
                     /locus_tag="ACHX5T_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFR8466126.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12498..13709)
                     /locus_tag="ACHX5T_000813"
     CDS             complement(12498..13709)
                     /locus_tag="ACHX5T_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR8466127.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13711..14283)
                     /locus_tag="ACHX5T_000814"
     CDS             complement(13711..14283)
                     /locus_tag="ACHX5T_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFR8466128.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14323..15480)
                     /locus_tag="ACHX5T_000815"
                     /pseudo
     CDS             complement(14323..15480)
                     /locus_tag="ACHX5T_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15781..16005)
                     /locus_tag="ACHX5T_000816"
     CDS             complement(15781..16005)
                     /locus_tag="ACHX5T_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466129.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(16046..16159)
                     /locus_tag="ACHX5T_000817"
     CDS             complement(16046..16159)
                     /locus_tag="ACHX5T_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR8466130.1"
                     /translation="MSRVGISEPHHAAGAIRLFLRHLKRYGALLAFRHGPA"
     gene            complement(16234..16308)
                     /locus_tag="ACHX5T_000818"
                     /pseudo
     CDS             complement(16234..16308)
                     /locus_tag="ACHX5T_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16318..16623)
                     /locus_tag="ACHX5T_000819"
     CDS             complement(16318..16623)
                     /locus_tag="ACHX5T_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466131.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16620..16898)
                     /locus_tag="ACHX5T_000820"
     CDS             complement(16620..16898)
                     /locus_tag="ACHX5T_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466132.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(17178..18926)
                     /locus_tag="ACHX5T_000821"
     CDS             complement(17178..18926)
                     /locus_tag="ACHX5T_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFR8466133.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18923..19222)
                     /locus_tag="ACHX5T_000822"
     CDS             complement(18923..19222)
                     /locus_tag="ACHX5T_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFR8466134.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(19228..19461)
                     /locus_tag="ACHX5T_000823"
     CDS             complement(19228..19461)
                     /locus_tag="ACHX5T_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466135.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19463..19683)
                     /locus_tag="ACHX5T_000824"
                     /pseudo
     CDS             complement(19463..19683)
                     /locus_tag="ACHX5T_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19676..19897)
                     /locus_tag="ACHX5T_000825"
     CDS             complement(19676..19897)
                     /locus_tag="ACHX5T_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466136.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19887..20087)
                     /locus_tag="ACHX5T_000826"
     CDS             complement(19887..20087)
                     /locus_tag="ACHX5T_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFR8466137.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            20217..20396
                     /locus_tag="ACHX5T_000827"
     CDS             20217..20396
                     /locus_tag="ACHX5T_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466138.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20521..20850
                     /locus_tag="ACHX5T_000828"
     CDS             20521..20850
                     /locus_tag="ACHX5T_000828"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466139.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20900..21088)
                     /locus_tag="ACHX5T_000829"
     CDS             complement(20900..21088)
                     /locus_tag="ACHX5T_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8466140.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21463..22692)
                     /locus_tag="ACHX5T_000830"
     CDS             complement(21463..22692)
                     /locus_tag="ACHX5T_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8466141.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGEKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22941..24062
                     /gene="roxA"
                     /locus_tag="ACHX5T_000831"
                     /gene_synonym="ycfD"
     CDS             22941..24062
                     /gene="roxA"
                     /locus_tag="ACHX5T_000831"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR8466142.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(24111..25337)
                     /gene="pepT"
                     /locus_tag="ACHX5T_000832"
     CDS             complement(24111..25337)
                     /gene="pepT"
                     /locus_tag="ACHX5T_000832"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR8466143.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25587..26723
                     /gene="potA"
                     /locus_tag="ACHX5T_000833"
     CDS             25587..26723
                     /gene="potA"
                     /locus_tag="ACHX5T_000833"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR8466144.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26707..27564
                     /gene="potB"
                     /locus_tag="ACHX5T_000834"
     CDS             26707..27564
                     /gene="potB"
                     /locus_tag="ACHX5T_000834"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR8466145.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27561..28355
                     /gene="potC"
                     /locus_tag="ACHX5T_000835"
     CDS             27561..28355
                     /gene="potC"
                     /locus_tag="ACHX5T_000835"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR8466146.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28352..29398
                     /gene="potD"
                     /locus_tag="ACHX5T_000836"
     CDS             28352..29398
                     /gene="potD"
                     /locus_tag="ACHX5T_000836"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR8466147.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29554..30375)
                     /gene="cobB"
                     /locus_tag="ACHX5T_000837"
     CDS             complement(29554..30375)
                     /gene="cobB"
                     /locus_tag="ACHX5T_000837"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR8466148.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30391..31302)
                     /gene="nagK"
                     /locus_tag="ACHX5T_000838"
     CDS             complement(30391..31302)
                     /gene="nagK"
                     /locus_tag="ACHX5T_000838"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR8466149.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31331..32575)
                     /gene="lolE"
                     /locus_tag="ACHX5T_000839"
     CDS             complement(31331..32575)
                     /gene="lolE"
                     /locus_tag="ACHX5T_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR8466150.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32575..33276)
                     /gene="lolD"
                     /locus_tag="ACHX5T_000840"
     CDS             complement(32575..33276)
                     /gene="lolD"
                     /locus_tag="ACHX5T_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR8466151.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33269..34468)
                     /gene="lolC"
                     /locus_tag="ACHX5T_000841"
     CDS             complement(33269..34468)
                     /gene="lolC"
                     /locus_tag="ACHX5T_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR8466152.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34730..35803
                     /locus_tag="ACHX5T_000842"
     CDS             34730..35803
                     /locus_tag="ACHX5T_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR8466153.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35931..39377
                     /gene="mfd"
                     /locus_tag="ACHX5T_000843"
     CDS             35931..39377
                     /gene="mfd"
                     /locus_tag="ACHX5T_000843"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR8466154.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39520..40482
                     /gene="ldtC"
                     /locus_tag="ACHX5T_000844"
     CDS             39520..40482
                     /gene="ldtC"
                     /locus_tag="ACHX5T_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFR8466155.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40565..40777)
                     /locus_tag="ACHX5T_000845"
     CDS             complement(40565..40777)
                     /locus_tag="ACHX5T_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HFR8466156.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         9601 a        10786 c        10094 g        10361 t
ORIGIN      
        1 tatgctcgat atgctattaa taatatccac ataaataaaa caacgggcgt gttatacgcc
       61 cgtttaaata tttaacacat gtagtgatta catattcttg atgatcgcat caccaaactc
      121 tgaacatttc agcagtttag cgccttccat cagacgctcg aagtcatagg ttacggtctt
      181 ggcgttgatc gcgccttcca tacctttaac aatcaggtct gcggcttcag tccagcccat
      241 atggcgtaac atcatctcag cggagagaat aatagagcca gggtttactt tgtcctgacc
      301 ggcgtatttc ggcgcagtac cgtgggtggc ttcaaacagg gcgcattcgt caccgatgtt
      361 tgctccaggg gcgataccga taccgccaac ctgcgctgcc agagcgtcag aaatgtagtc
      421 accgttcagg ttcatacagg cgataacatc atattcagcc ggacgcagca ggatctgttg
      481 caggaatgca tcagcaatca cgtctttaat gacgatctct ttgccggtat tcgggttttt
      541 aactttcagc cacgggccgc cgtcgatcag ttcaccgcca aattcttcac gcgccagctg
      601 gtagccccag tctttaaacg cgccttcggt gaacttcatg atgttgcctt tgtgcaccag
      661 ggtcacagag tcacgatcgt tagcaattgc gtattcgatc gctgcacgaa ccagacgttt
      721 ggtgccttct tcagaacacg gcttaatacc gataccgcaa tgttccggga agcgaatttt
      781 cttcacgccc atctcttcac gcaggaattt aatcactttc tcggcgtcgg cagagtcagc
      841 tttccattcg atacccgcat aaatgtcttc cgagttttca cggaagataa ccatatcggt
      901 cagttcaggg tgtttaaccg ggcttggagt gccctgatag taacgtaccg gacgcaggca
      961 gatgtagaga tccagttcct gacgcagggc aacgttgaga gaacgaatac cgccaccaac
     1021 cggagtggtc agcgggcctt taatggcaac gcgatattca cgaatcagat caagggtttc
     1081 agcaggcagc cagacatcct gaccataaac ctgtgtggat ttttcaccgg tgtaaatttc
     1141 catccaggag attttacgct cgcctttata ggctttctcg actgcagcgt cgaccacttt
     1201 cagcatggct ggggttacat ctacaccgat tccatcacct tcaatgtaag ggataatcgg
     1261 attttcagga acgttgagtt tgccgttttg cagggtgatc ttcttgcctt gtgccggaac
     1321 aactacttta ctttccattc acctctcctt cgagcgctac tggtttgctc gtctgccacc
     1381 acgttgcata tgcgtttgcg tcctgcgata cggatgcttt agagcaattt tttgttaatg
     1441 atttgtaatt gacttgtcaa tactacccta ttgtttgccg tcatgaaaga tgcgcgttat
     1501 taggctataa tgcggcaatt cataatctct gaaaatacca tgcaaaaaac ttcttttaga
     1561 aatcaccagg ttaagcgatt cagctcgcaa cgttctacca ggcgtaaaca tgaaaaccag
     1621 cccacgcgtg tgatcctctt caataaaccc tacgatgttc ttccgcagtt caccgatgaa
     1681 accggacgca aaacattaaa agaattcatc ccggttcagg gcgtttatgc agcaggtcgc
     1741 cttgaccgcg atagcgaagg gttgctggtg ctgaccaata acggcgcgtt gcaggcgcgt
     1801 ttaacccagc cgggtaaacg caccggaaaa atctattatg tgcaggtgga aggtattccc
     1861 acacaagacg cacttgaagc cttgcgcaat ggcgtaacct taaatgatgg ccctaccctg
     1921 cccgcaggcg cggagctggt tgacgaaccc gcgtggttat ggccgcggaa tccaccaatt
     1981 cgtgaacgca aaagtattcc caccagctgg ctgaagatca ccttatatga aggacgtaat
     2041 cgccaggtgc gccgcatgac cgcccatgtt ggtttcccca cgctgcgact gattcgctat
     2101 gcgatgggag attactcttt ggataatctt gccaatggcg aatggcgaga agtgacagat
     2161 taaggatgca aaatgtttaa accgcacgtt accgttgctt gcgtggtgca cgcagaaggc
     2221 aaatttttag tcgttgaaga gacgattaat ggtaaagcgt tatggaacca acctgccggg
     2281 catctggaag ccgatgaaac cttagtggaa gccgccgccc gtgaactgtg ggaagaaacc
     2341 ggcatcagcg cgcagccgca acactttatt cgtatgcatc agtggattgc gccagataaa
     2401 acgccgtttt tgcgtttcct ctttgccatt gagcttgagc aaatatgccc gacccagcct
     2461 catgacagcg atatcgactg ctgccgttgg gtcagcgccg aagaaatttt acaggcgtca
     2521 aatcttcgtt cgccgctggt ggcggaaagt attcgttgtt atcaaagcgg gcaacgttat
     2581 ccgctggaga tgattggcga ttttaactgg ccttttacaa agggtgtcat ctaaaaggct
     2641 gcgtgataga atacgccgcc ttgaagttca atgtcgtgag tgatccaatg tctgaaaccg
     2701 caaaaaaagt aatcgtcggc atgtccggcg gtgtcgattc ctccgtttct gcctggctgt
     2761 tgcaacaaca gggatatcag gtcgaaggcc tgtttatgaa gaactgggaa gaagacgacg
     2821 gtgaggaata ttgcacagca gcagcggatc tggctgatgc ccaggcggtc tgcgacaagc
     2881 tcggcattga actgcacacc gttaactttg ctgccgagta ctgggacaac gtcttcgaac
     2941 tgttccttgc cgaatataaa gccggtcgca cgccgaatcc ggatattctg tgcaacaagg
     3001 agatcaaatt taaagccttc ctcgaatttg ccgccgaaga tttaggtgcc gattatatcg
     3061 ctaccggtca ttacgtacgt cgggccgatg tcgatggcaa gagccgcctg ctgcgtggtc
     3121 tggacagcaa taaagaccag agctacttcc tttatacgct cagccatgag cagattgcgc
     3181 aaagcctgtt cccggtaggc gaactggaaa aaccgcaggt gcgtaagatt gctgaagatc
     3241 ttggtctggt caccgcgaag aaaaaagact ctaccggcat ttgcttcatt ggcgaacgta
     3301 aattccgcga gttcctgggc cgttatctcc cggcgcaacc gggcaaaatc attaccgtcg
     3361 atggcgatga aattggtgag caccaggggc tgatgtatca cactctcggt cagcgtaaag
     3421 gtctgggtat cggtggcacc aaagaaggta ccgaagaacc gtggtatgtg gtggacaaag
     3481 acgtcgaaaa caacattctg gttgtcgctc agggccatga acacccgcgg ctgatgtctg
     3541 tcgggttgat tgcccagcag ctgcactggg tcgatcgcga accattcacc ggcactatgc
     3601 gttgcacggt aaaaacccgc tatcgccaga ccgacattcc gtgcaccgtc aaggcgctgg
     3661 acgacgatcg cattaaagtg attttcgatg agccggttgc cgccgtgacg ccgggccagt
     3721 ctgccgtctt ctataacggt gaagtgtgcc tcggtggcgg tattattgag cagcgtctgc
     3781 cgctgccggt ctgattatta tctttactta aaacaaggaa gcagtgaacg tggcaaagaa
     3841 ttactatgac atcaccctcg ccctggccgg tatttgtcag tcggcacgcc tggtgcaaca
     3901 actcgctcac caggggcatt gtgatgccga tgcgctacac gtctcactca acagtattat
     3961 tgatatgaac cccagctcga cgctggcggt ttttggcggt agcgaagcca acctgcgcgt
     4021 cgggctggaa accctgctcg gcgtgctcaa tgccagcagt tgccagggct taaacgccga
     4081 attaacccgc tacacactca gcttgatggt gcttgagcgc aaactctcct cagcgaaagg
     4141 cgcgctcgac actctgggca accggatcaa cggcctgcaa cgccagctcg aacacttcga
     4201 tttacagtcc gaaacgctga tgagcgcgat ggctgctatc tatgttgatg tgatcagccc
     4261 gcttggcccg cgcattcagg tcaccggttc ccctgctgta ctgcaaagcc cacaagtgca
     4321 ggcgaaagtt cgcgcaaccc tgctggcagg cattcgcgcc gccgtgctct ggcaccaggt
     4381 cggcggcgga cgtctacaac tgatgttttc tcgtaatcgc ctgaccactc aggcaaaaca
     4441 aattcttgct catttaaccc cggagttgtg atctatggaa ttatcctcac tgaccgccgt
     4501 ttcccctgtc gatggacgct acggcgataa agtcagcgcg ctgcgcggga ttttcagcga
     4561 atatggtttg ctgaaattcc gtgtacaagt tgaagtacgt tggctgcaaa aactggccgc
     4621 gcacgcagcg atcaaggaag ttcctgcttt tgctgccgac gcaatcggtt accttgatgc
     4681 aattgtcgcc aatttcagcg aagaagatgc cgcacgcatc aaaaccatcg agcgtaccac
     4741 taaccacgac gttaaagcgg ttgagtattt cctgaaagaa aaagtggcgg agatcccgga
     4801 actgcacgcg gtttctgaat tcatccactt tgcctgtact tcggaagata tcaataacct
     4861 ctcccacgca ttaatgctga aaaccgcgcg tgatgaagtg atcctgccgt actggcgtca
     4921 actgattgat ggcattaaag atctcgccgt tcagtaccgc gatatcccgc tgctgtctcg
     4981 tacccacggt cagccagcca cgccgtcaac catcggtaaa gagatggcaa acgtcgccta
     5041 ccgtatggag cgccagcacc gccagcttaa ccaggtggag atcctcggca aaatcaacgg
     5101 cgcggtcggt aactataacg cccacatcgc cgcttacccg gaagttgact ggcatcagtt
     5161 cagcgaagag ttcgtcacct cgctgggtat tcaatggaac ccgtacacta cccagattga
     5221 accgcacgac tacattgccg aactgtttga ttgcgttgcg cgcttcaaca ccattctgat
     5281 cgactttgac cgtgacgtct ggggttatat cgcccttaac cacttcaaac agaaaaccat
     5341 tgctggtgag attggttctt ccaccatgcc gcataaagtt aacccgatcg acttcgaaaa
     5401 ctccgaaggg aatctgggcc tttccaacgc ggtattgcag catctggcaa gcaaactgcc
     5461 ggtttcccgc tggcagcgtg acctgaccga ctccaccgtg ctgcgtaacc tcggcgtggg
     5521 tatcggttat gcgctgattg cgtatcaatc caccctgaaa ggcgtgagca aactggaagt
     5581 gaaccgtgac catctgctgg atgagctgga tcacaactgg gaagtgctgg cagaaccaat
     5641 ccagacagtt atgcgtcgct atggcatcga aaaaccgtac gagaagctga aagagcttac
     5701 tcgcggtaag cgcgttgacg ccgaaggcat gaagcagttt atcgatggtc tggcgttgcc
     5761 agaagaagag aaagcccgcc tgaaagcgat gacgccggct aactatattg gtcgagctat
     5821 cacgatggtt gatgagctga aataagcctc aaatcagtgc cggatggcga tgctgtccgg
     5881 cctgcttatt aagattatcc actttttctt ttttcacttt acctcccctc cccgctggtt
     5941 tatttaatgt ttacccccat aaacacataa tcgcgttaca ctattttaat aattaagaca
     6001 gggagaaata aaaatgcgcg tactggttgt tgaagacaat gcgttgttac gtcaccacct
     6061 taaagttcag attcaggatg ctggtcatca ggtcgatgac gcagaagatg ccaaagaagc
     6121 cgattattat ctcaatgaac atttaccgga tattgcgatt gtcgatctcg gattgcctga
     6181 cgaggacggt ctgtcactga ttcgccgctg gcgtagcaac gatgtttcac tgccgattct
     6241 ggtattaacc gcccgtgaaa gctggcagga caaagtcgaa gtattaagtg ccggtgctga
     6301 tgattacgtg actaaaccgt ttcatattga agaggtgatg gcgcgaatgc aggcattaat
     6361 gcggcgtaat agcggtctgg cttcacaggt catttcgctc cccccgtttc aggttgatct
     6421 ctctcgccgt gaattatcta ttaatgacga agtgatcaaa ctgaccgcgt tcgaatacac
     6481 cattatggaa acgttgatac gcaataatgg caaagtggtc agcaaagatt cgttaatgct
     6541 acaactctat ccggatgcgg agctgcggga aagccatacc attgatgtac tgatgggacg
     6601 tctgcgcaaa aaaattcagg cacaatatcc gcaagaagtc attaccaccg ttcgcggcca
     6661 gggctacctg ttcgaattgc gctgatgaaa aaattactgc gtcttttttt cccgctctcg
     6721 ctgcgggtac gttttctgtt ggcaacggca gcggtagtac tggtgctttc gcttgcctac
     6781 ggaatggtcg cgctgatcgg ttatagcgtc agtttcgata aaactacgtt tcggctgtta
     6841 cgtggcgaga gcaatctgtt ctataccctt gcgcagtggg aaaacaataa gttgcatgtc
     6901 gagttacccg aaaatatcga caagcaaagc cccaccatga cgctaattta tgatgagaac
     6961 gggcagcttt tatgggcgca acgtgacgtg ccctggctga tgaaaatgat ccagcctgac
     7021 tggctgaaat cgaatggttt tcatgaaatt gaagcggatg ttaacgatac cagcctcttg
     7081 ctgagtggag atcattcgat acagcaacag ttgcaggaag tgcgggaaga tgatgacgac
     7141 gcggagatga cccactcggt ggcggtaaac gtctacccgg caacatcgcg gatgccaaaa
     7201 ttaaccattg tggtggtgga taccattccg gtggagctaa aaagttccta tatggtctgg
     7261 agctggttta tctatgtgct ctcagccaat ctgctgttag tgatcccgct gctgtgggtc
     7321 gccgcctggt ggagtttacg ccccatcgaa gccctggcaa aagaagtccg cgaactggaa
     7381 gaacataacc gcgaattgct caatccagcc acaacgcgag aactgaccag tctggtacga
     7441 aacctgaacc gattgttaaa aagtgaacgc gaacgttacg acaaataccg tacgacgctc
     7501 accgacctga cccatagtct gaaaacgcca ctggcggtgc tgcaaagtac gctgcgttct
     7561 ctgcgtagtg aaaagatgag cgtcagtgat gctgagccgg taatgctgga gcaaatcagc
     7621 cgcatttcac agcaaattgg ctactacctg catcgtgcca gtatgcgcgg cgggacattg
     7681 ctcagccgcg agctgcatcc ggtcgcccca ctgctggaca atctcacctc agcgctgaac
     7741 aaagtgtatc aacgcaaagg ggtcaatatc tctctcgata tttcgccaga gatcagcttt
     7801 gtcggtgagc agaacgattt tgtcgaggtg atgggcaacg tgctggataa tgcctgtaaa
     7861 tattgcctcg agtttgtcga aatttctgca aggcaaaccg acgagcatct ctatattgtg
     7921 gtcgaggatg atggccccgg tattccatta agcaagcgag aggtcatttt cgaccgtggt
     7981 caacgggttg atactttacg ccctgggcaa ggtgtagggc tggcggtagc ccgcgaaatc
     8041 accgagcaat atgagggtaa aatcgtcgcc ggagagagca tgctgggcgg tgcgcggatg
     8101 gaggtgattt ttggtcgcca gcattctgcg ccgaaagatg aataaatatg tccttttacc
     8161 actacatcaa gtcattgatt cgatcaaaca attttcattg aaaaagtgcc aaaaatcgca
     8221 aattgactac accattaact acaccgttcg gtgcactgta tgaaacaacg tggaacaaat
     8281 agacacaaga aatatacagg cgggtaatct ttccaggggg aagcgcacca attcatgagg
     8341 ggcgttaatg tcgatatggg gatccccata acggggctac cggatttttt ttccggttaa
     8401 cctttaatca ggctggtggg ttttacatac ccttgatcac cgtaatgatg atccagatgt
     8461 gcaccttttc caccaatggc ggaaaagctc caaccgtaac gatgatcgct atggttgcct
     8521 cgatggtctg cgtggtcatt atgaccgttc agccagtgac cttaatcaga atttttcgct
     8581 aaaaaatgac gttggccacg tcatccggaa agcacaaaat cacgtcgatt tttaatgaac
     8641 ggttaaaccg tctttttgtc acggtgtgtc actggttgtc actctccgtc acgattgagg
     8701 tggtggtcat tctgcccgac cgcgcatctt tgcgctatcg ggaatatcaa acagttacct
     8761 cgccgacttc ctcaaattga ggtttccgaa aatatcagag cgggttagcg attaagctga
     8821 cgagtaatta aactgcgaaa atttcgtagt ttctgaatat attccggcct gctggttaac
     8881 agtcctcaga tgtgagggat gtagccagcg caattttgcg ctttgcttta atctcagtga
     8941 gttaccgccg taaccgctcc ggcttcttcc agtggtacgt gattttctcc ttctcccgat
     9001 acagttcaac gcggcgattg taggccagca tttccagaac gcggatccgt atgtcgcgca
     9061 tatccgcatc attaagctgg ataccatcac ggcgcatcac ctcagcaaca acacgcgcat
     9121 aattttcggc tgtcacgctg tccggctgcg tggtctgttc gtcagcctgc tggctgattc
     9181 cagagacgcg gcggattaat cgcagtattt cggcttctgt catgctgctg acctcattac
     9241 accccgctaa attcttccag tttctggcgg tggctgtcgc ttatatcaaa agcaaaatcc
     9301 tcatgctctg cctggaatgt accaaacgcc atcagcgccg ctacgctcgg gtctatcttg
     9361 ttcggtgatt ttttcttgtt cggcttgata ttggcgttcg cgtcactctg catcacgacg
     9421 ttactcatcg accaggacaa caccggatcg ccacgatgca caatcaccct gcggttaaca
     9481 aaaacttcga acgatttcgc cgccggactg aatctgaggt aggtttgcgg gaacggctcc
     9541 acctcaaaac cagccccctg taattgcgtc ctgagatgcg tggcgttcca cgtatcaaag
     9601 cccaccagcc tgatattaaa tttctccgca tcctgcatga tgtcatctct gatccggtcg
     9661 taatcaatgc agtcacctgg cgttgtgcgt atccagcccg ctttagccca ctggcgatag
     9721 acagcgcggt ttttattggc ggggttctgt agctggaact ccggcagata atgacgggaa
     9781 atcagcataa tggttttacc gaccggaaag gcatagcata cgctggaaat atcgctggtt
     9841 gatgataagt ccagccccgc gtaacactcc tgaccgtgta aatcttcctc cgtgaacgtt
     9901 ccggcacact cagcccatgc accattaccc atccacgggg tagtcccctg acaccagata
     9961 ttaaatcgct ttgtcatcat ctccacccac tgcgacggaa taccccgcgc tttctggatg
    10021 gttgaggcca gtttttcacg atccacggaa acatcaatat tagggttcgc ctttatccac
    10081 atcgccggat catcaacctc gttttcgtcg tccagttcgt aaatcagtac aaaaattgaa
    10141 tcgttaacct cttcgccgtc caggatctgg cagcaataat catagtgctg tttacaggct
    10201 gaaacgacgt tactgcccga tgtggtaatg gcaaataaca gcccttcggg acgtgcgccc
    10261 atccccagct caagcgcgga ataaacgccg ttatcggggt gtaggtggta ttcgtccacg
    10321 atggaaagac tggggtttgt cccctcgatg gttgccgctt ttgctgccag cggctttaac
    10381 aggctgttgc ttttcgggtg tatcacctta tgcgcctgaa tattcacccg cctgcgtaac
    10441 ggtcgggata aaaggcacat ctgacgcgca ttatcaaaca cgatccgcgc ctggtcacgg
    10501 ctcaccgctg cggtgtagat atcctgctga ccgttctcca taatcagaaa ccagttagcc
    10561 agaatcgcgg cggtcgtgga tttcgcattt ttgcgcggca cttcgataaa ggcgctcgtg
    10621 tatttgcgcc gtccggtggc cttaacctta aagccgagga tgcacgcaaa ggcgaactgc
    10681 tgccacggct ccagctcaat gggtctgcca cgcatcggtc cttttacgtg cgggcacacc
    10741 ctggaaaagg caataaaccg ctccacaacc tcacgatcga acgtgtaaag ggggctttta
    10801 aggtccgaaa agtaccgttt aacagcctgt tttaaccgtt tacaggccgg aattttgccc
    10861 gtttttacgt cttctgcgta cttattccag gcggtcaagc tcgtcctctt cttctgtttc
    10921 cggtggattt ttacggcggc ttatcgggtc aaaaccgagc aaggaggcca tttttatcat
    10981 cactctttca gcgtcggatt ttgcgcttaa tgcggggttt ctgctctcgc cgccctgact
    11041 gttaacaatg ctgaacccgc gcgccgcaag gtctgcaacg gctttccggt aaatggagta
    11101 gttgacgcaa tacagttcca gattgctcca gtcggcgggg gtcaggtctc cccgttccgc
    11161 aagctgccgc gatttttccc gccactgctt cacggcgata tcatccaggt aggcggggtc
    11221 gggtttgtcc tgaaaacgcc ccccaccccg tcatacagcc tcatcagcga ttgcggaaac
    11281 attccataac ctcgcggtca cggtcggtta atcgcttcgc tgtgatgcgt tctgcgcgtc
    11341 ctgacgcttt atctttatgc cctgtttcct gtgtcttcca tgcgtcacgc tgccttataa
    11401 gtccacggat aaggcggttt tgttcccgct cattcatcag cgtcatacat ccagttattg
    11461 cggttagcag cccgttcttc ctcttcacga aatccacctg cggcacgctt gctttttgtt
    11521 gccggatcaa gccatttcgt tttctggtta tgacacgcct ggcacaatgg ttgatggttc
    11581 cattcgggcc agaagagaac atcatcaccg ccattaatcg ggataatgtg atccaccacc
    11641 acggcgggcg tatatatccc cttctcaagg catcgcacgc ataacgggtt tttactcaga
    11701 tacatggcgc ggtatttgtc ccactgtctg gaatatccac gcgcgcggcg gtgtcctcgt
    11761 ctggcatcct ctgcacgcca tgccgcccgc ctgtgctcct cacacttgcc ggatttaacg
    11821 cgcctgttac agcctggttc tgtgcatcgt cttaatggtt gccacggcat cagtaccccc
    11881 ccacatcacg gtaagccgtc cagagtgcgc ctatcgtcat gggtacgcgt gtttttgcgt
    11941 tatccgcgac aatctggcga ttttcataca ggtgagcgat aaacatcatg cagccaatct
    12001 ttatggctgg cgtgaacgcc agcccgtcat caaagcgcct gcctatgtgt atctggcacg
    12061 cctcaagcga tgcggcaatg tatccgctga ttaactggtc ttcatcgtcg ccatcgatgc
    12121 ggcaatggag tttcacttct tccagggtga taagttcttc tgtcattttt ccgcgccctc
    12181 acgacaaaga atttcaaggc gtgtcctggc ggcatccggc agcggctgcc cgatgatatt
    12241 cagtacacgc cccgccagcg gccccgtact gacctttatc cggctggtgg cgttgatgtc
    12301 cttccggtac cgtatccaga tccttacggt accaatcgcc agctctgcgc ccgatgaaat
    12361 ggcctccttg ctgctgacca tgttcacgct tgcccagagt gtgtgaccgt cctcccacgt
    12421 ttcgagtatt tcgccggtta tatccctggt ctgtttcagg gtctgaatcg ttatcctgtc
    12481 acgcagtcgc cctatgttca ttccggtttt tctccctcgc tgatttttac ttcctgtttc
    12541 catgcctggc tgaactcatc accaccatca cgcggtgaca gcccttcacg ttcgcgggct
    12601 tcgttcggac acataacgtc ggattttatg ccgcgctcat aggtggcaaa gcgttcgcca
    12661 ggtgtggccc gtaacaggtc cgcgctgtca aactccacct gataccggat accaggtaca
    12721 ggcgaggcca ccagcaaggc gttttttatc tgctgctcaa agttcgccag ccacgggcgc
    12781 atcgtcatgg tgagaaatgc gcggcttgcc tcgctgaagt tgctgtaggt gctgttgctg
    12841 tattcctgca aaaaaatcgg cgagacgttg aacattcggg cgatgtcttc aatggtgaag
    12901 cgacgggagg ccagccattc ggcatcctga ttactcatgc ccagctgctg atagctcatc
    12961 cccccttcaa ggatgggcgt ttttccggcg tttctggccc ctttgtagcg ttccagtgcg
    13021 gctaatgcct gtttgccctt cacgccgtcc agccattcgc ctgacgtgat aacccctgcc
    13081 gccatcatgc catcacgcat cacgctcgcg ccgtggcgtt gttgggccag cccaagcccc
    13141 agcgattcgc ggcagatggt tacaggtgag cgccccataa aaccgtcatc cgtggagtaa
    13201 cgaaggtgga gaacctccca cggtaaatag ttgcggatgt ttccggtgta ggcgtcagtg
    13261 atgcagtagc gccagttatg atcacctgtc tgctccacgt tcaccgactg cggcgggtaa
    13321 ggatgtaaag ccgccggaaa accatcacgc ccccactgaa taaccgcata agcattacca
    13381 cttaacaggc agtagcggat catcattcgc ttaaactggt agggtgtctg ccacgcgttc
    13441 gggcgctcgt tgaggatgtg atcgaccgga tgagaatcaa gccattcgcg ggcctccttc
    13501 cccttctcat tgcgtaccag gtacaggtaa cacggcatgg tagccaccgc gtcagagatg
    13561 accgtgacgg cgttcatgac ggcggggagt gattccgctg tcccagatga tacgtactca
    13621 cccgcccccg tgttcgatgt gccggccagc gccataaatt catcaagcgt catgctgcgc
    13681 tgctcttttt ttcgtctgaa aggccacata tcacaccccc gctaaatccg cccaccagcg
    13741 gcgattatcc gcacgcggca ttttttcggg gtgctgctca tacagggaac ggcgggccag
    13801 ctccacgccg gaatcggggt aagccggtac cgatgtaacg gtaatttcgt acagttccgc
    13861 caccagcacg gtgcgcacgc atggatctgt tgtggtatcc catacatcct tacgggagcg
    13921 aaagccaaag ctcatccccg agatgtcccc acgcttaacc agttcgataa cgtcgcgccc
    13981 tgtactggta tccggtgggg tcagttcaaa gcgtaaccct gtttcgtcct cttccagttt
    14041 cagcgtgccg gaacgggtgc gccccagtaa catgctgtgg tcatgctcat acaggccgcg
    14101 aacgtcatta cccgccgcaa gccattcagt aaacgccccc cgctggaatt tttcgtaaaa
    14161 ctcaccccat aacagttctg aaagcttatc ccagcgaaca acgtagcccg tcagcgtacc
    14221 ggctccgctg gtggtgattt ccgatgaccg gatttccata ctcttcataa ttttttcacc
    14281 cataaaacac taaaggggct ttttagcccc ttctgtatgc tgttaatcgt cgtcttccgg
    14341 cagtgccaga atcttgatcg cgttcgaatc caccacgcca ccgcctaaat atttttgcgt
    14401 gaaaattttg atgaatcctg gttctgtcag gttgtccggt ctggtgcgaa cacctgtttc
    14461 gtgatcaaca atgtagtaac cgcgtttgaa gtcacccagg gcaataacgt tatcaggcat
    14521 aaactccaga tattcgaccg gaaggcccag caacgtatca ggatcacccg cctgtaaacg
    14581 gtcgcgccag atataatcac cgttcgcgtt cttcaccttc tgaagttttg ccgccgtcgt
    14641 ggagttaacc actcagaccg cgtttttgcg gtatttttta cgtaatgcaa atttcaggtc
    14701 gatcagcggg tccgcagatg tccacgccag agattcggaa ggtttaatta cctgcaacgt
    14761 accaaaatca cgctctttgt cgttcttctc tgcacggggt acggataaaa aaccttttgc
    14821 ttttttgtca ccgtcgccca caaccagatc gctttcttcg gtttccgtga aggtgtcgcc
    14881 aatctcaccc gtcagccatg aaaggatgtc cacatcggaa aaatccacga tttcctgtgt
    14941 ggtgcgcggg tacgcataga ccggatacag cttaatgctc acctcgttaa tctgcggggt
    15001 gctggtctgt tcgcgtgcct taccctcttc accgtggtta acggtcgcac cgccagcgga
    15061 aacaagctgc ttaaactcgt tgctgctgat tttcttcacg gtacagatgc gacgcatggt
    15121 ggattcatcc gccagcattc gcatgatttc ggtgttcagt tcagggataa cggtataacc
    15181 accatcagcg ggaacgcctg tacttaatgc gcgggtttca ccggtcagaa tgtagttacg
    15241 tagttctgcg gggtcagtgg tctggctgct tttacctggc ttgctgcgct cttcgtctgc
    15301 aatggcttca aggcgggaaa tgtctttatc gagggattca gctttagcgc gtaattcgtc
    15361 aaattttgcg ccctcagcat cgttaagact gcggttttct ttttcagcgt tctccagcat
    15421 gtcgcgcatc tgatttttaa tggcggtttt ctgctggcgt aattcgatta ttctctgcat
    15481 aaaaaaagtc ctggggttaa gtaaggaact ccaggacgcg gcaaacactc aaccgttttt
    15541 cataaggaaa tcagtaatcg caccgatcgt tttcccgcct ggtaatgaat attggcgagc
    15601 acattaacag gcgggaaagt ggccccagcg tcctggcacc acgggcgaga ataatcatga
    15661 ttcagttcgg gtaaaatatg ccgatccgct cagtgaacaa cgtggaacaa ccacgaacaa
    15721 ataatttaca aaaaataaca aaaagccgga ttgctccggc tgtatgtgct gtatgggtgc
    15781 tcagtctctg atccctttca gaaattctat caatgcatct gtctgttcag gatttactgc
    15841 cagcatttca ccggatagcg cgcaacgaac aaaaccatga tgatcctttt caatcagcgc
    15901 ccccgtttcc agaaatgcgc ggtaatcagt gatattcatc gtctccatgt tgtcagcatg
    15961 gtatttatca cgctgtttaa gtatctcatc aaatttcatc ggcattgcgt tttcctctgt
    16021 tgtgtctgtt tgtttcagat agtaactatg ccggaccgtg acgaaaagcc agtagtgctc
    16081 cataccgttt caaatgacgc aaaaaaagcc ggattgctcc ggctgcgtgg tgtggttctg
    16141 atattcctac tctgctaatt tgctcagccc catgtctgcg taagtgcgat ttactgcatt
    16201 tctcaggtct gcgtagttct ccgttggcgg ctccggtgtg cccttgctgc tctgttttac
    16261 cagcgggtcg cccaccagct gcccgtaagc tgcaatttgt gctgtcatat tgccacctca
    16321 gtgaagcgat ccggcattgt tctttttcat ctcttccatc attttttcag tgagcattac
    16381 gcactgaaac cccatgtttt tgatgtctac gggtttcatg cctttgtcag tggatatcac
    16441 aggcagaata tcctgataca catcgcggtc agggtaacta agcacacaaa gctcccatga
    16501 tgccacgggt gaggatcctt cgtctgtttc aacccaccag cctggtgtag ctgcgcttat
    16561 ttgcagaatc gtgatccgtc cgttgtgctt gtaaaatttt tcaccaaatt ttggttcgct
    16621 catgattcca cctctccggt tttaacgttg attgttgtta cctgttccgc ttcggcaatc
    16681 tcccgctctg tcagcgtggc aaaatttgcc gccgtcgtgg tcatgaatgc gcttatcagt
    16741 tcgggatgtg ctttcgcgta tccttccccc gcgtggcggt ctatcgttct gattgccacc
    16801 tttaaagcgt gctctgtcat gtctaacgcg cgatatttcg gttctgttct gtctctgcgt
    16861 tttttgaatg atttattaaa tttccctgaa gtgtgcatat ttatttttac cccctcgttt
    16921 aaaaagtttt gagttgtgcc tccccttgtc taccttatct accttagtgg ccctcatgcc
    16981 agtaatggcg ctgctttcag cggggtagag tgcttttatc cactatctac cccgtgtcta
    17041 cctccctgtc tgattcaggt aaaatcaggt agagagggta gatagtgggt agacagtaaa
    17101 aaaaggctat ctacctaact taatgcactg aattaaatgt atttttcttt actcaggtag
    17161 acagcaatta caaaaaatta taaaaacgcg tcgcactcgt ctgttgttat tgcgttagtc
    17221 tgcgttactc ccttaacttt ccgcgtaata tattcatgtc cgtaaacttt cgcggctggc
    17281 ttcatagcct tgccaaagtc atttacgttt agcggtttgc tcctgcctgc gtacgccata
    17341 aacgccagat agacgcggta aaggctgttt ctggtcgtgt acttcactga atcgccaccg
    17401 ccgcccatca tcaggccgcg tgcttcctcc agaaaattca ggaactggca aaactcaata
    17461 accggatccg tctgttgctt tattgccaga gcttcatcac cgtcacgctg ttccagtagt
    17521 aaagcccgtg ctttctcaga gtcggtaaag ttcgccagca atcggcggat aatgacaggg
    17581 atttcagccg caatcttttc cggtagctcc ctgtcttttt cggcctcact aacgatattg
    17641 tcgaaacgga aaatcacgcg acgacgtgcc acacctccgg cccgttcggt gaatatcatc
    17701 gggttattgt tggtcgccag caccaccgcc ctgattacag ccgtgaaacg cttttcgtat
    17761 ttcgggttaa tttccacggg gtcaccgccc gtgattttct tgatgcccgt tccttcgcct
    17821 gtatatttcg gctggtcagc caggacgata agacgactcc cgacaacctg cgcacgtcca
    17881 ccagcatcat caagcgatgt catttcagcg cttaccgtgt tctgtttccc tgccagaagg
    17941 ctggctatgt gtgtgaatgt acttttaccg ctcccgccgt ctccggtggc ctcaataaac
    18001 atctgccagt cgtaccggtt cgccataatc atgtacagcg cggcacatat acgcatcatc
    18061 ttgcgctggt cttttccggc tgcgtgctca agccatttat gaaagtttag cgcgttatcg
    18121 cggatgttct cccctggtgc tggtggcgtg tactcaatgc cgttgtgcgt ggtgatccag
    18181 ttctccggcg tgtgcgggga aaattccccc gttttcaggt caagcgcacc attggcgaac
    18241 ggcagcaaat cgccggacgg ctcgcccatt ggttcggcaa taacttttaa cgcttccacg
    18301 gcgttattga ttacgcgctt gctgaaagtg gccctgtgct ctgaatagat caccaccatt
    18361 tcgcggctaa gttccattgt gctgaccgga caccataccc cgccgcgcca tacgtgaacg
    18421 atttcacttt cctgatgcac acaaacgcca tcaaagcgct cggcaagcag ctgcgcgcgc
    18481 tcactgtctg ccatttgtgc cagttgcgta tttttctgct ccggcatggt aagccctgcg
    18541 gcaatatttt cacgctcagc attcagatag cgccgccagt tttcacactt ctggacgtgc
    18601 attccttcgg ggtaaaaatt tgcgtcctga atatctgccg ccgccagctt ctgaccaatc
    18661 tttttggtct ccaccaaatc cagttctccg gcctggtaca gccttacgcg tttttttcca
    18721 tccggaacaa ttttcagtgc atcaagttcg gcaagctgat ttggcccaag ccacacaggc
    18781 ggcacattat cgccggatgc ggggccatcc tgctcctgcc attgtttcgc gtgcgaccag
    18841 gcatcactac ccgcaaagat aattacctct gtgtctttgt gttttattcc gcgtggctgt
    18901 ttttttacgt tcggtgccag tttcattttt tacccctgaa tacgttaagc atctttttta
    18961 tttcctgaat attggcgcgt gctttctccc tgctggttgg tttactgcgg ggcgctgcct
    19021 gtaccagcgt gaaatcacgc cggaattgat aaacaggcat cacgcaatca tattcgtaac
    19081 cttcacggcg gtaggtgatg cgccgttctg ccacgccttt aatcgttacc atgccgccgt
    19141 actggtcgcg gtaaatatca ccgcgcgtaa atttagggtg agtgttgcca ctggcagtta
    19201 agccagaata tttaagtttc attattttta ttctccggtg tgttgttctt tatatctgtc
    19261 gtgcaataga tctatttctt gcagttccat tattacaggc tcaagaagcg ttattaatgc
    19321 cgtggcaatt cttgattttt gtttgtcgcg ttcattgtcg ccaagtgttt caagccatat
    19381 gcgcaatatt tccagcatgt tttcactgtg agaaagtgca agaaatgcgc ggtctattgt
    19441 ttcgtggtaa atatcacgca tattaatccc cgtccgtcgt ttttcttaaa ataacctctg
    19501 tcacgaaatc agcataatcg gcggcgatat tcagtacatc aagcccgttg atttatattc
    19561 tctcgtggaa agtaagaaaa aagccgctct aataagttct ggcattgacg aaagcgcatc
    19621 agccgcatca tcaggaacgc cggaaaattc ctgtttcagg gaattaaaac gatcatcacg
    19681 cataaccccc cccattttca caatcggcaa caagaatatt tttagcgtca ttcagtgacc
    19741 gcgttgcgat gtatcggata cattccaggg cgaactgtgt ccggtctttc tgttcttctc
    19801 cctgcgcaag ctctgccgtg cattcaatat caataagcgc gtgcatcagc gtagtgagtg
    19861 cggcggctgc tgcgttcggt gtggttttat tgtgcatagg tttccccctg acgaatacgg
    19921 gcggcgaata cagcaacaca accggacggg caacggctac gcgcttcgcg ttccgtccaa
    19981 gcggttacgt ggatgatttg ggattctccg gcactcagtg ccagaaaacg ccacacaaag
    20041 gccgtttgtg tgtgtacaag gtgtggtata tgatttacgg caaccataac ggctcctagt
    20101 ttacgttgtt ggttagaagc cccgttactg ctcctacagt gcggggcttt gctttttgtg
    20161 ttgattgtgt gtaatgtgtc actacacata aatacattac ctgaggtgta attaacgtgt
    20221 caacacacaa aaacgaaagg cgtggtaatc ctccgttcca gttccgttta gatcctgact
    20281 tacgggaaaa gatggaaaag gcacaacaac aggacggcga tgattctctt gctgcctgga
    20341 taaagcgaat aatccgcaaa gaattacagc aacgcggcat agaacccaaa ggataaaaac
    20401 tatcagcgcc gtggtgtgag gtactacggc gcattgcttt acaggcgcac acaatgacca
    20461 acgaagaatc aaccgaaaaa acatcacctg cacgaaaaag acgacgcaga aagaaagagc
    20521 atgaatcaga acgattcgcg ccgtgttctt ttgcccttga gaagtacctt aaagaatact
    20581 catcaaagga gagaacttcg cagttatggc aacgcactga accagactga ttgcattgcc
    20641 caccagcctg atagcggcta tcatccccgt gcttatgttt gggatcacac acacaaacgg
    20701 cgcagcgggt tatctgttca gaaaggcggc tccatttcgg ggccgctttt tttatgcctg
    20761 aaaaacccca attttgtggt tttccagttt caccagggcg aacgaatccc cgcccacgtt
    20821 ctggcgtata ttcaatcttc atggttatag ctctgtgttc agatgattga tgtgtggcgg
    20881 ctgcgtgccg ccagcgtgat taatgaactg ccttgcagct atccttccag gccagaacct
    20941 cggataaaga ccagccaacg gaacgaccgc caagtttacg acgtgatggg aattgtccgg
    21001 ccttttccag gcggtagcgg catgagcggc taaggcctgt tagcttttcg cattcttttt
    21061 cacgtataaa ccgatcagtg cttaacacta ttgccccctt tcgtttctta aagagttatt
    21121 tcgtgttcta ttgcgttggg atgtgtctga ttgtgtcagg atgattcaga gttggcaaat
    21181 gttgaggccg tatggtttac agaaagagga ataatcagga taaaatcctt taaattcatg
    21241 ttaatacaaa ggcataaaat attgtttcat gccttttttc tcgctattta aagagtgatt
    21301 cgctagtgta taaaaaatca gtaacacatt aaaaatcagc tacttataaa tctgtacact
    21361 ttttcacctc ttgttcgtag ttgttccgca ttgttgctca ttgttgcacg ttgtatctgt
    21421 tcgcatatcc agtatgcgca tactgaaaaa acacgaaaaa aattattttc ttctggctac
    21481 tggtagcgtg gttacgtttt catgtgttcc cgccagtatc cctaaccgct ccgtccacat
    21541 atccagcgca ttgcgtttag catccagata acgggaatga ttataaactc gctgcattcc
    21601 tggcatctgg tggcctgtaa gctgctccac gacgtgagga tccacgccta aatcgttcag
    21661 catcgttgta aaggtgcgcc ggatgtcatg tagtgaccag tgagggtgat ttagcctcct
    21721 gtgcgctaat ctgccgtact gcgacacgct tgtttcctgt ttcacttccc ccagcaataa
    21781 gcccgtgtgc ctgttctgct ccaccagctg cgtgacgaac ggcagtattg cttccggtat
    21841 gggccggaat attgccacct tcgttttgct gtgttctttc ggcacggtcc agagcatttc
    21901 ggtaaaatcc cactcgctga tctccgataa cctcagttct accgtccggc atccgaacac
    21961 aatcaggagg cggattaacg cgatgtagta gggggagaat atttttttgt ccagtgcctg
    22021 caataattcg cccagttctt tggtgcttaa gacacgctcg cttatatccg gtttttcccc
    22081 aacgtccgcc acactcatat catcaagaac gttgctgatt gcatagcgcc gcctccggca
    22141 gaacttaagc gcctgtttgc acgtctgtag caagaatccg gcagtaacag gcgttcgctt
    22201 tgccacctgg tcaaaacagg ccagccagtg ccgtagctcg catttatcca gcggcatagc
    22261 accaatgtgc tgtattacgt gattattaag gcgctttttc agggcggcat aatccacgcg
    22321 gttttccttt gcgtacgact caagccagta ggtgagcgca tcgccaaccg ttaccggctt
    22381 taacgcttcc tgtacggtgt aattaagctc atgacgtgga tttttcccct ctgccagcca
    22441 tgcgcgacac tgggcggctt tttccctggc tgatttcagg ctcagatcag gataatttcc
    22501 cagcttaatg cgttccggtg gtgcccccct tcccgttccg gccctgtaag tgaaatacca
    22561 ggttaaaagg ccactggttg aatgcctgac gctcaggttt ccaccgtcat taagaaaggc
    22621 tgttttttgg gcgggtgtgc cgttgatttt cctcagctgt gtatcgctca gtttgttaag
    22681 tgctctgctc ataattttga ttccggtcat cacaattaac tacaccatta actacaccga
    22741 tcgttgcaca aagggctaca acgtgaaaca agctggaaca aggaaaatca caaatctgtt
    22801 tataatcaaa aacataatga acaatctgaa acattatgaa acggcaaaaa acactaattg
    22861 ataaaatatg tccatacttc acgcattacg ttaagcatcc gttataatcg gttgcagata
    22921 ccagcctgtg gatgcttaac atggaatacc aactcactct taactggccc gattttcttg
    22981 aacgtcactg gcagaaacgc ccggtggtgt taaaacgcgg ctttaataat tttattgacc
    23041 cgatctctcc agacgagttg gcgggtctgg cgatggaaag cgaagttgac agtcgactgg
    23101 tcagtcacca ggatggcaaa tggcaggtca gccacggccc gttcgaaagc tacgatcatc
    23161 tcggtgaaac caactggtca ttactggtac aggcagtaaa ccactggcat gagccgaccg
    23221 ccgcgctgat gcgaccgttc cgtgaactac cggactggcg tattgatgat ctgatgattt
    23281 ctttttctgt acctggcggc ggcgtcggcc cgcatctcga tcagtacgac gtgtttatca
    23341 ttcagggtac cggacgtcgt cgctggcgag tgggcgaaaa gctgcaaatg aaacagcact
    23401 gcccacatcc ggatctgtta caggtcgatc cgttcgaagc catcatcgat gaagagctgg
    23461 agcctggtga tattctttat attccgccag gattcccgca tgaaggctac gcgctggaaa
    23521 atgcgatgaa ctattccgtg ggttttcgcg cgccaaatac gcgggaatta attagcggat
    23581 ttgccgatta tgtgctgcaa cgtgaactgg gcggcaacta ctacagcgat ccggatgttc
    23641 cacctcgtgc tcatcctgcg gatgttctgc cgcaagagat ggataaactg cgtgagatga
    23701 tgctcgaatt gatcaaccag ccggaacact ttaagcaatg gtttggcgag tttatatccc
    23761 agtcacgtca tgaactggat atcgcgccgc cggaaccgcc ttatcagcca gatgaaatct
    23821 acgatgcgct gaaacaaggt gatgtgctgg tgcgcctggg tggtctgcgc gtattgcgca
    23881 ttggcgacga cgtgtatgcc aatggtgaga agatcgattc cccgcaccgt ccggcactgg
    23941 atgcactcgc cagcaacatt gcgctgactg cggagaattt tggcgatgcg ctggaagatc
    24001 cgtcattcct cgcgatgctc gcggcgctgg tcaatagcgg gtattggttc ttcgaagggt
    24061 aagtttggat ttaagccgga tgcagcatca catgccgcat cccttttcgc ttacttccgt
    24121 tgcgccgtta actcggcaat acggacgatc acctgcaccg ctttttccat accttccaga
    24181 atcacaaact catgcttacc atgatagttg taaccgccag tgaacaggtt cgggcaaggt
    24241 aatcccataa acgacaactg cgcgccgtcg gtaccgccgc ggatcggttt cagttccggt
    24301 tcaatatcgc agtcacgcat cgcctgctgg gcgatatcga gaatatgcgg atgctcaacc
    24361 actttctcgc gcatattgta gtaactgtct tcaatcacca attcaatgta gcaatcagga
    24421 tgtaaccctt tgcccacttt tttggcgatc tccatcattt tacgtttacg cgcttcaaac
    24481 tgtttacggt cgaaatcacg gatgatgtag tgcatatcgg cccgttcaac ggtgcctttc
    24541 atgctcgcca ggtgatagaa accttcatag ccttctgtca tttccgggct ttcatccgcc
    24601 ggaacttccg catgaatacg tgccgccagc gacagcgcat ttaccatcac tcctttcgcc
    24661 gtgcccggat gaacattgtt accgacaatt tttatattga ccgatgcggc gttgaagttt
    24721 tcaaactcca gttcgcctac gccaccaccg tcaacagtgt aagcccagcg ggcatcgaag
    24781 gcatcaacat caaaatgttt cgcccctttg cccacttctt catccggggt aaaggcgacg
    24841 cgaatatcac catgcggaat gtttttctgt tgcaataccg ccagcgcggt catgatttct
    24901 gcaatacctg ctttgtcatc agcacctaac aaggttttac catcggtggt aatcaacgtc
    24961 tgacccagta gctgatgcag caccgggaac ataaccggtg ataaaacttc atcgccgata
    25021 cccagcgcaa tatcgccacc gcgatagttt tcaacaattt gcggattaac atttttgccg
    25081 ctgcaatccg gtgaggtatc cacatgagaa ataaagccaa tcgccgggat atcgccaggg
    25141 acgttagccg gtaacgtcgc catcaaagtg cccttctcac ttaaggtcac attgataagc
    25201 cccatttctt cgagctgctc tttcagcaga tgcaataact tccattggcc ttccgtgctg
    25261 ggaacctgtc tcacccctgc ttttgattgg gtatccagag acacgtagtt caaaaatcgc
    25321 tcaagtagtt tatccatgta gtcaccctca ctttttgtga caacattatt aagaagcaag
    25381 aaaagacaaa tattgcgtca ggtcactttt acccctgcaa gcgggaatat ttatcagcat
    25441 taccttaatg aatataaagc taagccagta attcacaaca aagattggcg attcaagcgg
    25501 tttgccgtag aattaccgcc ctcattagga gtcaccaagg tggttaacca caaaccccgc
    25561 atcggtaagc catccgttgc gtttacatgg gacagagtaa aaaattgaat aaacaaccga
    25621 gttcgctttc accgctggtg caattggcgg gaattcgcaa atgctttgat ggtaaagagg
    25681 tcattcccca gctggatctg actatcaaca atggcgagtt cctcacgctg cttggccctt
    25741 ctggctgcgg taaaacaacc gttcttcgcc tgattgcagg tctggaaact gttgattccg
    25801 gacgcatcat gctggataac gaggacatca cccacgttcc ggcggaaaac cgctatgtga
    25861 acactgtttt ccaaagctat gcacttttcc cccacatgac cgtgttcgaa aatgtggcct
    25921 ttgggttgcg catgcaaaaa acccccgctg ctgaaattac gccccgcgtg atggaagccc
    25981 tgcggatggt gcagctggaa accttcgctc aacgtaaacc gcatcagctc tctggtggtc
    26041 aacagcaacg cgtcgccatt gctcgagcgg tggttaacaa gcctcgtctg ttgttgctgg
    26101 atgagtcgct ctcagcgctg gattacaaac tgcgtaagca aatgcagaac gagctgaaag
    26161 cgttacagcg taagcttggc attacattcg ttttcgtgac tcacgaccag gaagaagcac
    26221 tcaccatgtc ggacaggatt gtggtgatgc gcgatggtcg cattgagcaa gacggcacgc
    26281 cgcgtgaaat ctacgaagag ccgaaaaacc tgtttgttgc cgggttcatt ggcgaaatta
    26341 atatgtttaa cgccacggtg atcgagcgac tcgacgagca gcgcgtacgc gccaacgttg
    26401 aaggccgcga atgtaatatc tacgttaact tcgccgttga accggggcaa aaactgcatg
    26461 ttctgctgcg cccggaagac ttacgtgttg aagagattaa cgacgacaac cacgctgaag
    26521 ggctgattgg ttacgttcgt gagcgtaact acaaaggcat gacgctggag tcggttgttg
    26581 aactggaaaa tggcaagatg gtgatggtca gcgaattctt caatgaagac gatcctgact
    26641 ttgaccactc tctcgaccaa aaaatggcca ttaattgggt agaaagctgg gaggtcgtac
    26701 tggctgatga agaacacaag taagttccag aatgtagtga ttgtcactat tgtcggttgg
    26761 cttgtgttgt ttgtctttct gcccaacctg atgatcattg gcaccagctt tttgacccgc
    26821 gacgacgcca gtttcgtcaa aatggtcttt acactggata actacacgcg tctgctcgat
    26881 ccgctctatt ttgaagtgct attgcactcg ctgaatatgg cgctgatcgc caccctcgcc
    26941 tgcctggtgc tgggctatcc atttgcctgg tttctggcga agctgccaca caaggtgcgt
    27001 ccgctgctgc tgtttctgct gattgttccg ttctggacca actcattgat tcgtatctac
    27061 gggctgaaaa ttttcctcag caccaaaggc tatctcaacg agtttttgct ctggctgggc
    27121 gttatcgaca caccaatccg tatcatgttc acgcccagtg cggtgattat cggtctggtt
    27181 tacattctgc tgccgtttat ggtgatgccg ctgtactcca gtatcgaaaa actggataag
    27241 ccgttgcttg aggcggcgcg cgatctcggt gccagcaagt tacagacttt tatccgcatc
    27301 attattccac tgacgatgcc ggggattatt gccggatgtc tgctggtgat gctgccagcg
    27361 atgggcctgt tctatgtgtc cgacctgatg ggcggtgcga aaaacctgct gatcggtaac
    27421 gtcatcaagg tacagttcct caatattcgt gactggccgt ttggtgcagc caccagcatt
    27481 acgctgacta tcgtaatggg cctgatgttg ctggtttact ggcgcgcttc tcgtttgctg
    27541 aataagaagg tggaactcga atgatcggtc gactgcttcg cggcggtttt atgaccgcta
    27601 tctacgcgta cctgtatatc ccaatcatta ttttgattgt gaactccttt aacagctcgc
    27661 gctttggcat caactggcag ggttttacca ccaaatggta tagcctgctg atgaacaacg
    27721 acagcctgtt acaggcagcg cagcattcac taacaatggc ggtgttttcg gcgacgtttg
    27781 ctacgcttat cggttcactg acggcagttg cgctgtaccg ttatcgcttt cgcggtaagc
    27841 cgttcgttag cggaatgctg tttgtggtaa tgatgtcacc agatatcgtg atggcaattt
    27901 cgctgctggt gctgtttatg ctgctgggta ttcagcttgg cttctggtcg ctgctgttct
    27961 cgcatatcac cttctgcctg ccatttgtgg tggtgacggt gtattcgcgc ctgaaaggtt
    28021 ttgacgtgcg gatgctggaa gcggcgaaag atctcggtgc cagcgaattt accattctgc
    28081 gaaaaatcat tctgccactg gcaatgccag cggtggcggc gggctgggtg ttaagcttta
    28141 ccctgtcgat ggacgatgtg gtagtttctt cattcgtcac cggaccaagt tatgaaattc
    28201 tgccgttaaa aatttattcg atggtcaaag tcggcgtatc gccggaagtt aacgcgctgg
    28261 caaccatatt actggtgctg tcgctggtga tggttattgc cagccagctt attgctcgtg
    28321 ataaaacgaa aggtaacaca ggggacgtta aatgaaaaaa tggtcacgcc acctgctcgc
    28381 ggcgggtgct ctggcactgg gcatgagcgc cgctcacgcc gatgacaaca acacgctgta
    28441 tttctacaac tggaccgagt acgtgccgcc aggactgctt gaacagttca ccaaagaaac
    28501 cggtattaag gttatctatt cgacttacga gtcgaacgaa accatgtacg ccaagctgaa
    28561 aacgtacaaa gacggtgcct atgacctggt ggttccttca acctattacg tcgataaaat
    28621 gcgtaaagag gggatgatcc agaagatcga caagtcgaag ttaacgaact tcagcaatct
    28681 cgatccagac atgctcaaca agccttttga cccgaataac gactactcca ttccgtatat
    28741 ctggggtgcg acggcgattg gtgttaacgg tgatgcggtg gatccgaaat ctgtcaccag
    28801 ctgggccgat ctgtggaagc cagagtacaa aggcagcctg ctgttgaccg acgatgcccg
    28861 tgaagtgttc cagatggcgc tgcgtaagct gggctactcc ggtaacacca ccgatccgaa
    28921 agagattgaa gctgcatata acgagctgaa aaaactgatg ccaaacgtcg cagcgtttaa
    28981 ctccgataac ccggctaacc cgtacatgga aggcgaagtt aacctcggca tgatctggaa
    29041 cggttctgct ttcgttgcac gccaggcggg tacgcctatt gacgtggtgt ggccgaaaga
    29101 aggcggcata ttctggatgg acagcctggc gatcccggca aatgccaaaa acaaagaagg
    29161 cgcgctgaaa ttgatcaact tcctgctgcg cccggatgtg gcaaaacagg ttgctgaaac
    29221 tatcggttat ccaacgccaa accttgcggc gcgtaagctg ttaagtccag aagtggcaaa
    29281 cgataagacg ctctacccgg atgctgaaac cattaaaaat ggcgaatggc agaatgacgt
    29341 tggcgcagcc agcagcattt atgaagagta ttatcagaag ctgaaagcag gacgttaatt
    29401 cagcaagcgg gcggtggtat taccgcccgt tttttagtta gcaaggatgt ggctaatgag
    29461 tcaggacagt aaagttttat cccgctcttt cgtcggtatt gtggtccgct tgtaggcctg
    29521 ataagcgtag cgcatcaggc aatgcttcct gctttataat ccctccagca acttttcaac
    29581 aaattctggc accacctggc ttgccgggcc gtaatatttc tcggcaaatt cattaccaac
    29641 ctgactcggt tcaagattca gttcaacggt gtgagcacca tgtagtttgg cttcgtgtac
    29701 aaacccagcc gccggataga catgcccgga agtaccaatg gcaataaaga catccgccat
    29761 cgacaacgcc atataaattt catccatgcc gagtggcatt tcgccaaacc aaacgacgtg
    29821 cgggcgcaag ggtgccggga actggcaaca atggcattta tcttctggcg taacgtctcc
    29881 ggtccagtcg agaacctgac cactttgcga acaacgaact ttcagcaatt ccccatgcat
    29941 atgaatgaca ttggtattac ctgcgcgttc atgcaggttg tctatattct gcgttaccag
    30001 caaaaagcga tcgccgagag cgtcttgcag tttagccagc gcaagatgcg cggcgttagg
    30061 ctgaatttct ggctgctgca gctgtcgacg acgggcgtta taaaacgctt gcaccagttc
    30121 aggatcgcga tcgaaacctt ccggcgttgc cacatcttca acccgatgtt cttcccacag
    30181 gccatctgcg gcgcgaaagg tacgaatacc tgattccgca gaaattcctg cccctgtcag
    30241 tacgagtact cttggttttt ccattgcttc cggcaccact ttatctctga aaaaaatacg
    30301 ctgacgcaaa cgctcgcgca ggcgacgttt atttttacga aaacgactta accgatgacc
    30361 ccgacgcgac agcatagcaa cctctgttgt ttaatcggtt agatgtagga aggccgcacc
    30421 acgcattcct cccgcatcac cgtggcgcgc gcgttcaatg cgtggaacac gagctacagg
    30481 taagagatga caaggcaacc tgtccgccag ttgcgttgtg attgccggga aattcgataa
    30541 gccaccacca atgacgacca ggtcagggtc aacaatggtc aggatatttc ccagagaaac
    30601 cgctaataaa tccagataac gctcaacgtg cgcccttgcc tgctcatcgc cttgatcata
    30661 aagcgcaata atttcgggag cctgcaacgg ttgatgataa tagtgttgat acagccacgc
    30721 aaaaccgcga ccagacagat aattttcaat gcagccatgc tgaccacagc cgcagcggcg
    30781 taacgggaaa tccaacccca tcatggttaa cgcatcaacc ggcagacgca tatggccaaa
    30841 ctcgccggta atgtagcttt taccagtaat cggtttgccg ttgaaaacca gcccgccgcc
    30901 aacgccggtg ccgagaatta accccatcac cagtggatat tgcgtaaatt catcatccca
    30961 ggcttctgaa agggcaaaac agttggcatc gttatcaagg cgtacatcgc gatcaagacg
    31021 tgcgctcagg tcggcacgca gcggtttgcc gctggcagcg gggacattag cggcatacag
    31081 cttaccatct tctgtttccg gcattcccgg aataccgatg ccgacagaac ctttacagcc
    31141 aaaacgttgg tcagcttcag ctaccagctc acacactgca tctaaaaatg cgtcatagct
    31201 gtcacgcggt gtcggcaccc gcttttccca ctgcaactgc cgaccgctat caaacacgcc
    31261 aagcgcaatt tttgttccac caatatcaaa cccgtaatac attgccgctc cttgttttaa
    31321 tgtactgcct ttactggccg ctaaggactc gcgcagggtc aatattactg gcgcgccgcg
    31381 ccggatacca acttgccaaa agactcagca acaatgctgt gaccagtacg tagaagacgt
    31441 ccagccagtg caattccgat ggcaggaagt caataaaata aatatcgctg gagaggaact
    31501 gatgaccgat cagcttttca atccactcaa taatcggggt aagttgcagt gaaacaacta
    31561 cgccgataac caccccacac aggctgccga acagccctgc cagcaatcca taccagacaa
    31621 agatggcgcg aattaaacca tctttcgccc ccagcgttct taatactgcg atatcgccac
    31681 tcttgtcttt caccgccatc actaaggtgg agacgatgtt gaaacaggcc acgccaatca
    31741 ccagtaccat cgccagatac ataatggcgc ggatcatctg gatatcgcga tacatatagc
    31801 cgtaagtacc aatccaactt ttaatataaa catagctgtt ggtcacttca cccgcatcgc
    31861 gtaccagctt attggcgttg aaaacatccg tcattttaag ggcaatacct gacacgctgg
    31921 aacccatatc aagatattgt tgggcatccg ccagcgggat catggcaaaa ctgtgatcga
    31981 gttgaccact caactgcaaa ataccggcaa tgtgcaaacg cacacgtttt ggctgcatca
    32041 gtttatgctc gggattcgag ttggggatca tgatcgacac ccaatcgccc tgcttcactt
    32101 tcagcgcatc cgccacgcct ttgccgatga taatttgctg ttcgcccgct ttaaaattgc
    32161 gccacgcatc cccctgaaca aacgagggta atgcgctcag acgctgttcc tgttgtgggt
    32221 taacgccctt cacctggatt gcgcgcagat tcgctccact ttccaccaga ccggtgaaat
    32281 tgatatacgg cgcagcggcg gcaatacccg gcactttctg cacgttatcc agtgcttcct
    32341 gccagttagt ccatggctga tttaccgctt cgatttcgcc atgcggcacc accgccagaa
    32401 tgcggttatt cagttcgcgt tcaaagccgt tcatcgcgct taagccgacg atcaataccg
    32461 ccacgccgag ggcaatgcca atggtagaaa tgacagagat tagcgacacc atgccgccgc
    32521 gtcgccgtcc gcgactaaaa cgcaggccaa tcaataacga taaaggcatc gccattactc
    32581 cgcccccatc aggctcagtt ccgccgtcag acgtccatca cgcatttcca gttggcggct
    32641 catacgtttc gccagttgca ggtcgtgagt gaccaccagg aaagcggtgc cctgcaagcg
    32701 attcaactcc ccaagcaact ggaagatgct gtcggcgtta cgcgcatcga ggttaccggt
    32761 aggttcatcc gccagtacca gacgcggatt attgaccagc gcacgggcaa tcgctacacg
    32821 ctggcgttcg ccgccagaaa gttcagatgg gcggtgattc gcacgatgct ccagccccac
    32881 cgcttttaac atctccagtg cacagctgtt gatttcagcg ggctttttct tgccaatcag
    32941 cagtggcata gccacgtttt ccagggcagt aaaatccggc agcaggtggt gaaactgata
    33001 aataaagccc agcttctggt tgcgcagttc tgctttcgcc gctgaagaca gtttgctcat
    33061 cggctgaccg ttaaagatca catcgccgga ggttggcgta tccagcccgc ctaacaggtg
    33121 cagcaaggta cttttaccgg aaccagagct accgacaatt gccatcattt caccttcgcc
    33181 cacgctgaaa ctgacattga gcagcacatc ggtttgcaca ctgccttcct gatagcgttt
    33241 gcacaggttg tcgcattgca acaggatctt attcataacg taaagcctca gcgggttgtg
    33301 tggcggcagc gcgccatgaa gggtaaagcg tagacagcag cgcgatagcc atcgccacca
    33361 gcgcaataac aatgacctgt aaaggttcga tagccaccgg cagcgccgcg ccatcaagca
    33421 ggacgccgat tatcggcatc agattattta actggctggc aagcagtgcg ccaagcgccg
    33481 ctccgaggat tgcaccgata atcccggcgc tagccccttg caccataaag accatcatga
    33541 tttgtcgcgg agttaagcct tgcgtttgca ggatcgcgac ttcgccctgc ttctccatca
    33601 ccatcagccc cagcgaggta ataatgttaa acgccgcaac agcgacaatc aggctcagca
    33661 gtaagcccat catatttttt tccatgcgta cggcctggaa cagctcgcct ttacggtcac
    33721 gccagtcctg ccatttgctg ccttcaggca gtttttgctg acttaaagag tcaactctca
    33781 gcggctcatc cagccacaaa cgccagccgg taatattggc tgccggataa cgcatcagac
    33841 gcgaggcatc ctcaatattc accagcattt catagccatc gacttcactg ttggcggcga
    33901 aagtgccaat cacattgaac aggcgctgac ttggaatacg ccccatcggc gtgaactggc
    33961 tggcagatgg caccatcacg cggatttgat caccgcgatt gacgcccaac tgtgaggcaa
    34021 gttgttcgcc gaggatgaca ttatatttcc ccggctcgag gtcagtttgt ttcacattga
    34081 ccagatacgg cgttagtgga tctttttgcg ccgggtcgat accgagcatc accccgaccg
    34141 ccacgctgcg cgcgctttgc agtaccacat caccggtagt aataggtgcg acgcgattaa
    34201 cgccgtccag tttgactgcc gtttccggga gttgctgcgg gttaagagag ccatgctcag
    34261 aagagagaat tgcctgtggc atcaggccaa ggatgttgtt ttgcagctcg cgctcaaagc
    34321 cgttcatcac tgacaatact gtgaccagcg ccatcacccc gagggtaatg ccgatggtag
    34381 aaagccagga gacgaaacga ccgaagcgat ccactgcacg cccacgcatg taacgcaggc
    34441 caataaatag agcgacaggt tggtacatga aatccgtctg gttgctgtag caaagacacg
    34501 agtatataag cgaaagcaag atgggtaaac gtctgaactg taagtgatcg tcagggctaa
    34561 tctgaaaagc ccccgtaaat caatcggcta aattgccata ttttatcatc aatcttgccg
    34621 cctgatctta atctgaaaaa tcatccaata cagacagata cccattacac ctcgggtcat
    34681 aaccagcagg atcaaccgca tgtaaattaa aaaagagcgt gttgttctga tgaaacaaaa
    34741 agagctatgg attaaccaga tcaaagggtt atgtatttgt ctggtggtga tttatcactc
    34801 ggtcattacc ttttatccgc atatgaccac ttttcagcat ccgttatcgg aagtcctgag
    34861 caaatgctgg atctatttca atctttacct tgcccccttt cgtatgccgg tttttttctt
    34921 tatatccggc tatttgattc gccgctatat cgacagcgta ccgtggggaa attgtctcga
    34981 taaacggatc tggaacatct tctgggtgct ggcgctttgg ggcgtggtgc agtggctggc
    35041 gttaagtgcg ctgaaccagt ggctggcacc tgagcgcgat ttaagcaatg cctccaatgc
    35101 cgcttatgcc gattccaccg gtgagttcct gcacgggatg atcaccgcca gcaccagctt
    35161 gtggtatctg tatgcgttaa ttgtctattt cgtggtatgt aaaattttta gccgtctggc
    35221 gctgccacta ttcgccttgt ttgtactgct gagtgtggcg gttaatttcg ttcccacgcc
    35281 gtggtgggga atgaacagtg tgatacgcaa tttgccttat tacagccttg gcgcatggtt
    35341 tggcgcaaca ataatgacct gtgttaaaga ggtgccgttg cgccgccatc tgctgatggc
    35401 ttctttgctt accgttctgg cggtcggtgc ctggttgttt actatctcgc tgctgttgtc
    35461 gctggtatcg attgtggtga tcatgaagct gttttatcag tacgagcaac gattcggtat
    35521 gcgctccacc agcctgctga atgtgattgg ctccaacacc attgctatct acaccaccca
    35581 tcgcattctg gttgaaatat tcagcttaac tctgcttgcg caaatgaacg cagcacgctg
    35641 gtcgccgcaa gtcgaactga cactcctgct ggtttatccc tttgtcagtt tgttcatctg
    35701 tactgttgcg ggcttgctgg taagaaaact ttcacagcgt gcattcagcg atctgttgtt
    35761 ctccccgcct tcactgcccg ctgccgtcag ttactctcgt taaaccatca ccccagccgg
    35821 ggcgattcaa tttgctaaac catgtcgatt acggataatg aacagcattg cttatcagag
    35881 atatgccccc atatgttgag gcatatccta acgagaatct gacaaccgtt atgcctgaac
    35941 aatatcgtta tacgctgccc gtcaaagcgg gtgagcagcg tctgctgggc gagttaaccg
    36001 gcgcagcctg tgcaacgctg gtagcggaaa ttgccgaacg tcacgccggt ccggtggtac
    36061 tcattgcacc agatatgcaa aatgctctgc gtttgcatga tgaaatcagc cagttcaccg
    36121 atcagatggt gatgaatctg gcggactggg aaactcttcc ctacgacagt ttttcgcctc
    36181 atcaggacat tatctcctcg cgcctttcca ccctttacca gctaccgacg atgcagcgtg
    36241 gcatactgat tgttccggtg aatacgctta tgcagcgcgt ttgcccacac agttttctcc
    36301 acggtcatgc gctggtgatg aaaaaaggtc agcgcctgtc acgagatgca ttacgaaccc
    36361 aactggacag cgccggttat cgccatgttg accaggtgat ggagcacggc gaatacgcca
    36421 cgcgcggcgc gttgctggat ctcttcccga tggggagtga gctgccttat cgtcttgatt
    36481 tctttgatga tgaaatcgac agcctgcggg tgtttgacgt cgacagccag cgcacgctgg
    36541 aggaagtaga agcgatcaat ctgctgcccg cgcacgaatt tccgaccgat aaagcggcaa
    36601 ttgaactgtt ccgcagccag tggcgcgata ccttcgaagt gaagcgcgat ccggaacata
    36661 tttaccagca agtgagtaaa ggcacattac ctgctgggat cgagtactgg cagccattgt
    36721 tcttcagcga accactgcca ccgctgttca gttatttccc tgccaatacc ttgctcgtga
    36781 atactggcga tctggaaacc agtgccgaac gtttccaggc tgacacgctg gcgcgttttg
    36841 agaatcgcgg cgtcgatccg atgcgcccgc tgttgccacc acaatcgctc tggctgcggg
    36901 tggacgagct cttctcagag ctgaaaaact ggccccgagt gcagctaaaa actgaacatt
    36961 taccgacaaa agccgcgaat gccaatttag gtttccagaa actgccagac ctggccgttc
    37021 aggcgcaaca aaaagcgccg ctggatgcgc tgcgtaagtt cctcgagact ttcgacggtc
    37081 cggtggtgtt ctcggtagaa agtgaaggtc gccgtgaagc gctgggtgaa ctgctcgcac
    37141 gaattaaaat tgctccgcaa cgcattatgc gtcttgatga agccagcgac cgtgggcgtt
    37201 atctgatgat tggcgctgcc gaacatggtt ttgtcgataa ggcgcgtaat ctggcgctga
    37261 tctgcgaaag cgatctgctc ggtgaacgtg ttgcccgccg tcgtcaggat tctcgccgca
    37321 ccatcaaccc cgatacactg atccgtaacc ttgcggagct gcatattggt cagccggtgg
    37381 tccatctgga gcacggcgtc ggtcgctatg ccggaatgac cacgctggaa gctggcggca
    37441 ttactggcga gtacctgatg ctcacctatg ccaacgacgc caaactgtat gttccggtgt
    37501 cgtcactgca tctgattagc cgttacgcag gtggcgcgga agaaaacgcc ccgctgcata
    37561 aacttggcgg cgatgcgtgg tcacgcgcgc ggcagaaagc ggcggaaaaa gtgcgtgatg
    37621 tggcggcgga attgctggat atctacgcgc aacgcgccgc caaagagggc ttcgcgttta
    37681 aacacgatcg tgagcagtat cagttattct gcgacagctt cccgtttgaa accacgccgg
    37741 atcaggcgca ggccattaat gcggtactta gcgacatgtg tcagccgctg gcaatggatc
    37801 gtctggtgtg cggcgatgtt ggctttggta aaacagaagt ggcgatgcgc gccgctttcc
    37861 tggcagtaga taaccacaag caggtagcgg tgttggtgcc taccaccctt cttgcgcagc
    37921 agcattacga caacttccgc gaccgtttcg ccaactggcc ggtacgtatc gaaatgatct
    37981 cccgtttccg cagcgccaaa gagcagacgc aaatccttgc ggaagtggcg gaagggaaaa
    38041 tcgatattct gatcggtacg cacaaactgc tgcaaagtga cgtcaagttt aaagatttag
    38101 gcctgctgat tgtcgatgaa gaacaccgct tcggggtgcg tcataaagag cgcattaaag
    38161 cgatgcgcgc gaacgtggat attctgacgc ttactgcaac gccgatccca cgtacgctga
    38221 atatggcaat gagcggaatg cgtgacctgt cgattatcgc cacgccgccc gcccgtcgtc
    38281 tggcagttaa aacctttgtc cgtgagtatg acagcctggt ggtccgggag gcgatcctgc
    38341 gtgaaatttt gcgcggagga caggtttatt atctctacaa tgatgtggaa aacattcaga
    38401 aagccgccga acggctggca gaactggtgc ctgaagcacg gattgccatc ggtcacgggc
    38461 agatgcgcga gcgcgaactg gaacgggtga tgaatgattt ccatcatcaa cgtttcaacg
    38521 tgctggtttg taccaccatt atcgaaaccg ggatcgacat cccgacagcc aacaccatta
    38581 tcattgaacg tgcggatcac ttcggtctgg cgcagctgca ccagttacgc ggtcgcgtcg
    38641 gacgttcaca tcatcaggca tatgcatggc tgctgacgcc gcatccaaaa gcgatgacta
    38701 ccgatgcaca aaaacgtctt gaagcgattg cctcgctgga agatctcggg gcaggttttg
    38761 cgctggcaac gcacgatctg gagattcgcg gcgcgggtga actgcttggc gaagaacaaa
    38821 gcggctcaat ggaaaccatc ggtttctcgc tgtatatgga gttgctggaa aacgccgtcg
    38881 atgcactgaa agccggacgc gagccgtcgc tggaagatct caccagccag caaacagaag
    38941 tcgagctgcg gatgccgtcg ctattgccag atgatttcat ccctgacgtg aacacgcgtc
    39001 tgtcgttcta caaacgtatt gccagcgcca aaacggaaaa cgaactggaa gagatcaaag
    39061 tcgagcttat cgatcgcttc ggcctgctgc cagatccggc gcgtaccctg ctggatattg
    39121 cccgtctgcg ccagcaagcg cagaaactgg ggatcaggaa gctggaaggt aatgagaaag
    39181 gcggggtgat cgaatttgcc gagaagaatc acgttaatcc ggcctggttg attggtttgc
    39241 tgcaaaaaca gccgcagcat taccgccttg atggtccgac gcgcctgaaa tttattcagg
    39301 atttgagtga gcggaaaacg cgtatcgaat gggtacgcca gtttatgcgt gaactggaag
    39361 agaacgcgat cgcttaatgt aaatcggggg cgtttctgcg cccccatctg caacatttac
    39421 aaattctttg cactttcctg cactatccga cactgtcacc atccataatt caggcttatc
    39481 tgtttattac aataacctta tatttattat gtatttttgg tgatgatcaa aacgcgtttt
    39541 tctcgctggc taacgttttt tacgttcgcc gctgccgtgg cgctggcgct accggcaaaa
    39601 gccaacacct ggccgctgcc gccagcgagc agtcgtctgg ttggcgaaaa caaatttcat
    39661 gtggtggaaa atgacggtgg ttctctggaa gccatcgcca aaaaatataa cgtcggcttt
    39721 ctcgctctgt tacaagctaa ccccggcgtt gatccttacg taccgcgcgc gggcagcgtg
    39781 ttaacgatcc cgttgcaaac cctacttcca gacgcgccgc gcgaaggcat tgtgatcaac
    39841 attgcggagc tgcgtctcta ttactacccg ccgggtaaaa attcggtaac cgtgtatcca
    39901 ataggtattg gtcagttagg tggtgacacg ctgacaccga caatggtgac caccgtttca
    39961 gacaaacgtg caaacccaac ctggacgcca acggcaaaca tccgcgcccg ttataaagca
    40021 cagggaattg agttgcctgc ggtagtgccg gctggaccgg ataacccaat gggccatcat
    40081 gcgattcgtc tggcggccta tggcggcgtt tatttgcttc acggtacgaa cgccgatttc
    40141 ggcattggca tgcgggtaag ttctggctgt attcgtctgc gggatgacga tatcaaaaca
    40201 ctctttagcc aggtcacccc aggcaccaaa gtgaatatca tcaacactcc gataaaagtc
    40261 tctgccgaac caaacggtgc gcgtctggtt gaagtacatc agccgctgtc agagaagatt
    40321 gatgacgatc cgcagctgct gccaattacg ctgaatagcg caatgcaatc atttaaagat
    40381 gcagcacaaa ctgacgctga agtgatgcaa catgtgatgg atgtccgttc cgggatgccg
    40441 gtggatgtcc gccgtcatca agtgagccca caaactctgt aaatcctgga taaaaaaaat
    40501 ccccgcgaca cggcatgtcg cggggaaaaa atcagcagta gcaggcatta atgagggtta
    40561 atgcttattt ataaattact gctgttccat ggagggtatt cggaccggtt acagaagtaa
    40621 tacggaaaga ttttgcgccc atctcatccg ctttttgcgc cagctgctct tccagcgatc
    40681 ccagatttgt ccccgcgtta gcactgatag taccgacttt ttgttggcct tctggcgttg
    40741 actgaacttc gacagccgca aagttggcaa atgacatgga gcttaaaatc gccgcagcga
    40801 tgaggttttt acgtttttca taatagtggc cttatgcaga tg
//
LOCUS       DAZSIO010000018        38775 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZSIO010000018 DAZSIO010000000
VERSION     DAZSIO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.018"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..155
                     /locus_tag="ACHX5T_000846"
     CDS             <1..155
                     /locus_tag="ACHX5T_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR8466157.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /gene="uidR"
                     /locus_tag="ACHX5T_000847"
     CDS             384..974
                     /gene="uidR"
                     /locus_tag="ACHX5T_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR8466158.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3065
                     /gene="uidA"
                     /locus_tag="ACHX5T_000848"
     CDS             1362..3065
                     /gene="uidA"
                     /locus_tag="ACHX5T_000848"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR8466159.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="ACHX5T_000849"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="ACHX5T_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="ACHX5T_000850"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="ACHX5T_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR8466160.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="ACHX5T_000851"
     CDS             complement(5626..7134)
                     /locus_tag="ACHX5T_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR8466161.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="ACHX5T_000852"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="ACHX5T_000852"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR8466162.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /gene="fumB"
                     /locus_tag="ACHX5T_000853"
     CDS             8609..10255
                     /gene="fumB"
                     /locus_tag="ACHX5T_000853"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR8466163.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="ACHX5T_000854"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="ACHX5T_000854"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR8466164.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="ACHX5T_000855"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="ACHX5T_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR8466165.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="ACHX5T_000856"
     CDS             complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="ACHX5T_000856"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR8466166.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14283..15602)
                     /locus_tag="ACHX5T_000857"
     CDS             complement(14283..15602)
                     /locus_tag="ACHX5T_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HFR8466167.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="ACHX5T_000858"
     CDS             complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="ACHX5T_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR8466168.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16699..17034
                     /locus_tag="ACHX5T_000859"
     CDS             16699..17034
                     /locus_tag="ACHX5T_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR8466169.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17031..17753)
                     /gene="folM"
                     /locus_tag="ACHX5T_000860"
     CDS             complement(17031..17753)
                     /gene="folM"
                     /locus_tag="ACHX5T_000860"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR8466170.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17790..19172)
                     /locus_tag="ACHX5T_000861"
     CDS             complement(17790..19172)
                     /locus_tag="ACHX5T_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR8466171.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19358..20302)
                     /gene="ydgH"
                     /locus_tag="ACHX5T_000862"
     CDS             complement(19358..20302)
                     /gene="ydgH"
                     /locus_tag="ACHX5T_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR8466172.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20826..22358
                     /gene="pntA"
                     /locus_tag="ACHX5T_000863"
     CDS             20826..22358
                     /gene="pntA"
                     /locus_tag="ACHX5T_000863"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR8466173.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22369..23757
                     /gene="pntB"
                     /locus_tag="ACHX5T_000864"
     CDS             22369..23757
                     /gene="pntB"
                     /locus_tag="ACHX5T_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR8466174.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="ACHX5T_000865"
     CDS             complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="ACHX5T_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR8466175.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25227..25592
                     /gene="mdtJ"
                     /locus_tag="ACHX5T_000866"
     CDS             25227..25592
                     /gene="mdtJ"
                     /locus_tag="ACHX5T_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR8466176.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25579..25908
                     /gene="mdtI"
                     /locus_tag="ACHX5T_000867"
     CDS             25579..25908
                     /gene="mdtI"
                     /locus_tag="ACHX5T_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR8466177.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25947..26768)
                     /locus_tag="ACHX5T_000868"
     CDS             complement(25947..26768)
                     /locus_tag="ACHX5T_000868"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR8466178.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26868..26951)
                     /locus_tag="ACHX5T_000869"
     CDS             complement(26868..26951)
                     /locus_tag="ACHX5T_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466179.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27044..27352)
                     /gene="asr"
                     /locus_tag="ACHX5T_000870"
     CDS             complement(27044..27352)
                     /gene="asr"
                     /locus_tag="ACHX5T_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR8466180.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQPAKPAAQPAA
                     "
     gene            27623..27727
                     /locus_tag="ACHX5T_000871"
     CDS             27623..27727
                     /locus_tag="ACHX5T_000871"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR8466181.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(27776..29029)
                     /locus_tag="ACHX5T_000872"
                     /pseudo
     CDS             complement(27776..29029)
                     /locus_tag="ACHX5T_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29136..30038
                     /locus_tag="ACHX5T_000873"
     CDS             29136..30038
                     /locus_tag="ACHX5T_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8466182.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAKEPFVFFDPHVGTGLYDDILGLMRR
                     YHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWL
                     VWPKHHEQSPAARNFRIHLLNALR"
     gene            30173..31393
                     /gene="mlc"
                     /locus_tag="ACHX5T_000874"
                     /gene_synonym="dgsA"
     CDS             30173..31393
                     /gene="mlc"
                     /locus_tag="ACHX5T_000874"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR8466183.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31518..32213
                     /gene="bioD"
                     /locus_tag="ACHX5T_000875"
     CDS             31518..32213
                     /gene="bioD"
                     /locus_tag="ACHX5T_000875"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR8466184.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32166..33458)
                     /gene="clcB"
                     /locus_tag="ACHX5T_000876"
     CDS             complement(32166..33458)
                     /gene="clcB"
                     /locus_tag="ACHX5T_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFR8466185.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33617..34231)
                     /gene="dmsD"
                     /locus_tag="ACHX5T_000877"
     CDS             complement(33617..34231)
                     /gene="dmsD"
                     /locus_tag="ACHX5T_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR8466186.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34274..35128)
                     /gene="ynfH"
                     /locus_tag="ACHX5T_000878"
     CDS             complement(34274..35128)
                     /gene="ynfH"
                     /locus_tag="ACHX5T_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR8466187.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35130..35747)
                     /gene="ynfG"
                     /locus_tag="ACHX5T_000879"
     CDS             complement(35130..35747)
                     /gene="ynfG"
                     /locus_tag="ACHX5T_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFR8466188.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35758..36593)
                     /locus_tag="ACHX5T_000880"
                     /pseudo
     CDS             complement(35758..36593)
                     /locus_tag="ACHX5T_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
     gene            complement(36654..37688)
                     /locus_tag="ACHX5T_000881"
                     /pseudo
     CDS             complement(36654..37688)
                     /locus_tag="ACHX5T_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
     gene            37746..>38775
                     /locus_tag="ACHX5T_000882"
     CDS             37746..>38775
                     /locus_tag="ACHX5T_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR8466189.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRD
                     SMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVE"
BASE COUNT         9902 a         9391 c         9926 g         9556 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaac actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaggcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggtatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctag
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtga
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgtcag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    13261 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    13321 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg
    13381 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    13441 cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg
    13501 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    13561 gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    13621 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    13681 tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa
    13741 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    13801 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    13861 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    13921 agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    13981 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca
    14041 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt
    14101 tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt
    14161 aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc
    14221 cccggtgtat tcatattcag taaattcaat tttccaccgg ggcttgttta ttatgctaag
    14281 gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct
    14341 gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg
    14401 atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg
    14461 acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc
    14521 gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt
    14581 ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg
    14641 ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat
    14701 gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt
    14761 acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc
    14821 gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa
    14881 ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca
    14941 taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg
    15001 atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca
    15061 ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc
    15121 agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca
    15181 gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca
    15241 tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac
    15301 aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta
    15361 ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta
    15421 acataaccgt taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga
    15481 atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg
    15541 gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc
    15601 acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa
    15661 tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat
    15721 agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct
    15781 tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt
    15841 gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    15901 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    15961 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    16021 cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg
    16081 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta
    16141 acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca
    16201 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    16261 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    16321 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    16381 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    16441 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    16501 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    16561 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    16621 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    16681 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    16741 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    16801 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg cattgaagcc
    16861 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    16921 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    16981 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    17041 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa
    17101 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    17161 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    17221 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    17281 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    17341 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    17401 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    17461 agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga
    17521 ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    17581 tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc
    17641 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    17701 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc
    17761 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    17821 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    17881 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat
    17941 aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg
    18001 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    18061 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    18121 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg
    18181 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    18241 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    18301 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    18361 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa
    18421 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    18481 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    18541 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt
    18601 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    18661 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    18721 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    18781 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    18841 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    18901 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    18961 ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    19021 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    19081 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    19141 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    19201 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    19261 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    19321 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    19381 cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt
    19441 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    19501 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    19561 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    19621 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    19681 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    19741 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    19801 tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac
    19861 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    19921 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    19981 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    20041 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    20101 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    20161 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    20221 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    20281 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    20341 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    20401 atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt
    20461 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    20521 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    20581 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    20641 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    20701 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    20761 gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    20821 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    20881 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    20941 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    21001 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    21061 aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    21121 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    21181 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    21241 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg
    21301 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg
    21361 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    21421 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    21481 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    21541 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    21601 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    21661 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg
    21721 aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    21781 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    21841 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    21901 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    21961 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    22021 cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg
    22081 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    22141 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg
    22201 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta
    22261 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    22321 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    22381 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    22441 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    22501 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    22561 gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca
    22621 gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    22681 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    22741 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc
    22801 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    22861 ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc
    22921 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    22981 ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac
    23041 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    23101 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    23161 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    23221 gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    23281 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    23341 cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc
    23401 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    23461 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    23521 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    23581 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    23641 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    23701 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    23761 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    23821 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    23881 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    23941 tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    24001 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    24061 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    24121 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    24181 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    24241 actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    24301 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    24361 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    24421 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg
    24481 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    24541 ccgcgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    24601 gatgattgtc atcaaaatcg acactgccag tacacgcggc acacgccagc ggaccatatg
    24661 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    24721 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    24781 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt
    24841 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    24901 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    24961 ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat
    25021 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    25081 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
    25141 tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
    25201 aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
    25261 ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg
    25321 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
    25381 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
    25441 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
    25501 taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
    25561 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
    25621 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
    25681 aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag
    25741 gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc
    25801 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
    25861 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc
    25921 ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
    25981 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
    26041 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
    26101 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
    26161 acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga
    26221 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
    26281 aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac
    26341 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
    26401 gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac
    26461 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
    26521 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
    26581 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
    26641 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
    26701 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
    26761 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
    26821 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
    26881 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
    26941 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
    27001 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
    27061 cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg
    27121 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
    27181 gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg
    27241 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
    27301 cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac
    27361 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
    27421 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
    27481 taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct accaataata
    27541 gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca
    27601 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    27661 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    27721 ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg
    27781 cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    27841 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    27901 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc
    27961 gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg
    28021 agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac
    28081 caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca
    28141 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    28201 ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca
    28261 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    28321 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa
    28381 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    28441 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
    28501 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
    28561 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
    28621 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    28681 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    28741 catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca
    28801 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
    28861 gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga
    28921 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    28981 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    29041 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct
    29101 aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc
    29161 gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata
    29221 tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac
    29281 tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag
    29341 atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc
    29401 agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg
    29461 tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg
    29521 aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc
    29581 tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta
    29641 tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac
    29701 tggctgaact ggcgaaagaa ccctttgtct tttttgatcc gcacgtcggg actgggctgt
    29761 atgacgatat tctcggactg atgcgacgtt accatttgac gcccgtcatc actcaggagg
    29821 tgggcgaggc aatgaccatc atcggtctgg tttccgccgg tctgggtgtt tcaattttgc
    29881 ctgcgtcatt taaacgtgtt cagctcaacg aaatgcgctg ggtgccgatt gctgaagagg
    29941 atgcggtttc tgaaatgtgg ttggtctggc cgaaacatca tgaacaaagt ccagctgcgc
    30001 gtaactttcg tattcatctg ctgaatgctc tcaggtgagg gaaatttcag cgaaaaagcc
    30061 cgaaaaatgt gctgttaatc acatgcctaa gtaaaaattt gacgacacgt attgaagtgc
    30121 ttcaccatag cctacagatt atttcggagc gcgaaaatat agggagtatg cggtggttgc
    30181 tgaaaaccag cctgggcaca ttgatcaaat aaagcagacc aacgcgggcg cggtttatcg
    30241 cctgattgat cagcttggtc cagtctcgcg tatcgatctt tcccgtctgg cgcagctggc
    30301 tcctgccagt atcactaaaa ttgtccgtga gatgctcgaa gcacacctgg tgcgagagct
    30361 ggaaatcaaa gaagcgggga accgtggccg tccggcggtg gggctggtgg ttgaaactga
    30421 agcctggcac tatctttctc tgcgcattag tcgcggggag attttccttg ctctgcgcga
    30481 tctgagcagc aaactggtgg tggaagagtc gcaggaactg gcgttaaaag atgacttgcc
    30541 attgctggat cgtatcattt cccatatcga tcagtttttt atccgccacc agaaaaaact
    30601 tgagcgtcta acttcgattg ccataaccct gccgggaatt attgatacgg aaaatggtat
    30661 tgtacatcgc atgccgttct acgaggatgt aaaagagatg ccgctcggcg aggcgctgga
    30721 gcagcatacc ggcgtaccgg tttatattca gcatgatatc agcgcatgga cgatggcaga
    30781 ggccttgttt ggtgcctcac gcggggcgcg cgatgtgatt caggtggtta tcgatcacaa
    30841 cgtgggggtg ggcgtcatta ccgatggtca tctgctacac gcaggcagca gtagtctcgt
    30901 ggaaataggc cacacacagg tcgacccata tggtaaacgc tgttattgcg ggaaccacgg
    30961 ctgcctcgaa accatcgcca gtgtggacag tattcttgag ctggcacagc tgcgtctcaa
    31021 tcaatccatg agctcgatgt tacatgggca accgttaacc gtggactcat tgtgtcaggc
    31081 ggcattgcgc ggcgatctac tggcaaaaga catcattatc ggggtgggcg cgcatgtagg
    31141 gcgcattctt gccatcatgg tgaatttatt taacccacaa aaaatactga ttggctcacc
    31201 gttaagtaaa gcggcagata tcctcttccc ggtcatctca gacagcatcc gtcagcaggc
    31261 ccttcctgcg tatagtcagc acattagcgt tgagagtact cagttttcta accagggcac
    31321 gatggcaggc gctgcactgg taaaagacgc gatgtataac ggttctttgt tgattcgtct
    31381 gttgcagggt taacattttt taactgttct accaaaattt gcgctatctc aatttgggcc
    31441 aggaaagcat aacttagact ttcaaggtta attattttcc tagtttatat ttgtgaagca
    31501 taacggtgga gttagtgatg ctgaagcgtt tctttattac cggtacagac acttctgtag
    31561 ggaaaacggt ggtttcccgc gcattgctac aagcgttagc ctcccaggga aaaagggttg
    31621 cgggatataa acccgtagcg aaggggagca aagagacacc cgaagggctg cgtaataaag
    31681 atgccttggt gttgcagagt gtttcaacca tcgaactgcc ttatgaagca gttaatccta
    31741 tcgcgttaag cgaagaagaa agtagcgtgg cgcacagttg cccaatcaat tacaccctca
    31801 tttcaaacgg cctggcaaac ctgaccgata aagtcgatca tgtcgtggta gaagggactg
    31861 gcggctggcg cagtctgatg aatgatttgc gtccactctc tgaatgggta gtgcaggaac
    31921 aactgccggt gttgatggtt gtcggtattc aggaaggttg cattaaccat gcactgctaa
    31981 cagctcaggc gatcgccaac gacgggctgc cgctcattgg ctgggtggct aaccgaatca
    32041 acccaggact ggcgcattat gcggaaatca ttgatgtgct gggtaaaaaa cttccggcac
    32101 cgctcattgg tgaactgcct tatctgccgc gcgccgaaca gcgtgaactg gggcaataca
    32161 tccgcttagc tatgctgcgc agtgtgctgg cggtagatag agtcacggtg taacgtccgc
    32221 gaaattaccg acgcaattac gcaggcaatc aataaaccgg ggagtagctg atactccccg
    32281 gtcatttcac atatcatcaa cgtcgacata atcggcgcgt gcgtggttgc cgccaacagt
    32341 gtcgccatcc cggtcaatcc gagtaaaagc gtaatttctt cgccatcagg gaaccataat
    32401 cccaggctac gaccatacaa catgccaatg gcaagaccga taaatagcgt cggtgtaaag
    32461 acaccaccgg gtgcgccgga accgctactc gccagcacgg cacacagttt acaaaggaag
    32521 atcccggcaa tgatcattaa cagtggtggg gcggttaaaa aggattgtac ggtgctatag
    32581 ccgttgcccc acactgcagg tgtgaacagg gaaagcagac ccacgatcaa cccgcctagt
    32641 gccagttgcc agggcggcgc aagtttgaga ctcacaaatc cacgatgaca ggcgttcatt
    32701 aacgttaaca acagtggtcc gcacagacct gccagcacac ctgtactgat aatcagcgca
    32761 tagtcacgag cctgaaccgt cactgagagt tgtacgttgt agagtaacgc gtcgctatga
    32821 ttaatcagat tgctaaccag ccatgccacg atggcggaaa taatcaccgg gccgagagag
    32881 gccaacatca tagtgccaaa cagcacttcg gctataaata aactgccagc aagcggggca
    32941 cgataggccg cagccattcc cgccgcggcc ccacaggcga tccataattt ccattcctgg
    33001 cgtggcgtaa aacgttgggc aaaacaggag gcggcaaggg cagctaaaag aatcatcgca
    33061 ccttcgcgac caattgcact gccgctggtt actaccagca gcgaggcaag cgatttaacc
    33121 aggcttgctg cgtaatcgaa ctgtccatcg gtttgcaacg cttccatgta atcggtcggc
    33181 gcatgagggc gttgttgggt aaatttctgc cagcccatca gcaacaaacc cgccgccagt
    33241 ccgccgagcg ccggagttag cagccgtcgc caggaggaaa ggtttgtcgc tgcattgacc
    33301 agactgccgg agtcattatt gaggaacaac cactccagta gcagcatcgc atgacgaaac
    33361 ccggcaacgg caaagacagc gagaataccg acgactgttg cgataagcag acggcggaac
    33421 atagtgcgca gatccggata gatgtgtaga tgatgcatga gcagaaatgg gcaatgaaac
    33481 acgggcgact attttgcggc aaaagaggcc aaccgcaaaa gcagtgcgtg ctttaagaga
    33541 taaaaaaacg tgacattgta acgtattatt gccgaatcgt gcgtaatcgt caccatccgg
    33601 caatattacg gtgatcctat cgaaagagtg gtttaaccgc gacaggaatc aacagttgtg
    33661 attgccattg cgccagcgtt aagcgagcca gttcacccag tgcacggtag aaagggtgtt
    33721 ctgctttttc gataaaaaca tcaagaaaac gtgttgacca cggaaaaagg tgccatgcca
    33781 gcaattcttc gcattctgtc tggcgaccat tctccgccaa ccacgcagcc atcaacagca
    33841 gtgaaccaaa atgatcttcc ggttcgtttt gcttcatttc aaactgaatg cctttctcgc
    33901 gcatccactg acgaagtgcc aatgttgaat cgccaaacag cacagattcg cgatccagcc
    33961 agaccgaccc ccacggcgga gacggcagtg cccacgggcc gacaaacaaa cgctgccagg
    34021 cctgggcgtg agtctcttca cactgtgtct gaaaagccgt caccagcggc gctaatgacg
    34081 cttctggtag aggccactga gtttcccagc cgtcactggt gagtaccgca accagaggtg
    34141 cggcttccgc actctctgga gcgtaataaa acagcgcgcc caacacgcgc gccgcgacag
    34201 aaaaattatc ttgctgtgaa aaatgggtca ttcctaacat cctgaaatgt gcgggttgcc
    34261 ccgcaccaat ttgttaacct gcaatagcca ttcctactgt catgtgcagg ccataaaaga
    34321 gcacacgccc gatcatctca ccaacaagta tgaggattag ccccagaaca agtccggcaa
    34381 cgtgaggttc acggcgacga atgagcggac aaagccagca tccaagacct gcgcataaca
    34441 ataccacacg ccagacctgt aaggaggcgt aatctggtac cagtgcgctg gcttgttgca
    34501 cggatgagtg aatagacgcc agagacaaac cttgcagcac aatcacctcg gcacaagcga
    34561 tcagcgccag aacgctaatg atggcaaatg gtgtggtatt aaatgtaacg cgggctgccc
    34621 gcagaattgc ggcagcaagt attggaccgc tcaacaatac cgtcaggaag aaagccagcg
    34681 tagtgtaacc gttatgccag gttggcacgg tgtcgatttg atacacgcag gtcatcatcc
    34741 agacgaaaat gacgccgagc gccatgctga caagtaacca gagtttcccc aacgcttgcg
    34801 gcattttacc gatgaccgcc accagccacc atagtccgcc aacggcaaaa aatatggaac
    34861 cggcagcaat ctcgttactc agaccagaag ctccgattcg attcagcgag ttaaacgctc
    34921 gcagtggcga tcccaggtgc atgacagagg caataaaccc gatgcccatc aacagccaga
    34981 ggaaaaacat gccacgaaca atgtgctgac ggtctgcgtc atttttggcg gcgaaccagc
    35041 caattccgct aacaatcagc gcgcctacta cgcattgacc caatacggta aagataacca
    35101 gtggccattc atgccatcca tttcccattt tacacttcct ccggattagc cagataacca
    35161 gtggtatcgc cggtcgggcg gctgttggcg ttaggtttga taacgatatt gggttttgtg
    35221 aaatgcgcgc gcggcagtgg cgcgacggca gccagagtgc cgtgcttctg gcgcagctct
    35281 tcaattggac cgaactcaag tgcacgcagc gggcaggatt cgacgcatat gggttgtttc
    35341 ccctcggcga cgcgcgaata acaaccatcg cacttcgtca tgtgcccttt ttcagcattg
    35401 tactgtggtg cgccgtacgg acaagccatg tggcagtagc ggcagccgat acaaacatct
    35461 tcatcaacca ccacaaagcc atcttcacgc ttatgcattg cgccgctggg gcagactttt
    35521 gtacatgcgg ggtcatcgca atggttacag gagatggaga gataataagc gaacacgttc
    35581 tggtgccaga tgccattatc ttcctgccag tcgccgccag catattcata aatacggcgg
    35641 aaactgactt ccgggccaag atctttaaaa tctttgcacg ccagttcgca ggttttacag
    35701 ccagtacagc ggctggaatc gataaaaaat ccatattgtg tggtcatggg ctactcctta
    35761 aaccttttcg atctggacaa gattgctgtg cgacgggttt ccctttgcca gcggtgaagg
    35821 gcggtgagag gtcagaatat tgatactgcc gccgtgatcg acccggtcac caaacatatc
    35881 cgctttaagc cacgcacctt gcccgatggc ggtaacgcca ggcagaatac gcggagtcac
    35941 ttttgcggca atcagcattt ctccattatt gttaaatacc cgcacggtat cgccatgacg
    36001 gataccgcgt gcctgagcat caatggggtt gatccacacc tcttgtgggc aggcctgctg
    36061 taacacatca atattgccgt agctggagtg ggtactacgc ctgttccgta caggacatgt
    36121 acagcctcat tgccgtaatc tttcaggatc cgccgaagat tatcactgat ggtatccagg
    36181 gcttcgtccc aacttatccg ttcaaattta ccttcaccgc gctcgccgac gcgcttcatg
    36241 ggatatttca acctgtcagg atgattcatc cgtcggcgga tagagcgccc gcgtaaacat
    36301 gcccgaacct gatgattacc gtaaacgtcg tcacctgtcg tatcagactc cacccagtac
    36361 acggtgtcat ctttcacatg caaacgtagc agacagcggc tcccgcagtt aacggtgcag
    36421 gaactccaga ccgttttctc ttctaccgga gcctctgccg cccggaccat ttgggaaaat
    36481 ggcagagtga aagcactgct tgccagcgcg agactgccaa gtgcggaggt tttcatcaga
    36541 cttctatggc tgatttcagc cttcatgagc gcctctgtgg tatggatttt catcattact
    36601 cacttattgc ttttcaaaca aaatgtcatg ccagaattta tggttgtagt gggttatatt
    36661 ttttcgatct cgaccagatt agtgtgctgc gggtttccct tcgccagtgg tgaagggcgc
    36721 agagtggtta gcgtattcac acatccgcca tggtcgattt tatcgccaga catattgggc
    36781 tcgtgccagg ctccctggcc catagcgcta actccaggga gaatgcgtgg tgttactttg
    36841 gctggtagcc gaacttcgcc acgatggtta aacacccgca ccatatcgcc gttggcaatc
    36901 ccacgtttct gtgcatctat agggttgatc cacacctcct gacggcaggc tgccttcagg
    36961 acatcaatgt tgccgtaggt cgagtgagta cgggatttgt aatggaaacc aaacagttgc
    37021 agtgggaagg ttctacgttc aggggagtcc cagccttcaa aggttgaggc ataaactggc
    37081 agtgggctta tcacttcatc tttttccagt tcccaggtac gggcaatttc cgccagcttg
    37141 ctggaataaa tttcaatctt accggaaggc gttttaagtg gatttgcctc ggggtcgtca
    37201 cgaaatgctt tgtaggcgac aaaatggcca ttgggatctt tacgcttata gatacccatt
    37261 tttttcagtt cgtcgtaaga cggtaacgcc ggatctttgg caagcatttt ggcgtacaga
    37321 tgttgtaacc attgttcctg cgtgcgacct tctgtgaact tttgatagac gtcaggtcca
    37381 agacgtttcg cgacttcact caggatccag taaatcggtt tacgttcgaa tttttcgctg
    37441 gtgactggct ggaggaaaat gagatatccc atgttaccgg cgtagtcgtt aggaataata
    37501 tcttcctgct caacggtcat caggtcttcc agcagaatgt cggcatattt tgccgatgag
    37561 gtcataaagt tttcgatgac cacaatcatt tcgcatttcg attcgtcctg cagaatttca
    37621 tgcgttttgt tgatgtcaga atgctgatta acgagggtat ttcccgcgta gttccagatg
    37681 gactccattg ccagacggat ggatggactt gaagtagttg actcaatgct acttaaggtc
    37741 ctgatatgac tgacattaat catgataaca ctgtatatcc atccagttct ttctctgggt
    37801 ctccctatcg gtatcagcaa gtctacacct caagccctga cacaaggctg acacaaatca
    37861 atctactcag cgtagctaag cctcagttag tccgcagagc caatggtcga tatactatcc
    37921 gcttcagact caagggacag accacaccgt ttctttcagt atcgacccgc agcacagaca
    37981 ggagagtagc cacaatgcgt cagagagagc ttgcagccac cgctaaggcc ttcatgttgg
    38041 ataggcctga agtctcacta caggagctta cagagcatct ccggtctatg gctgagcagt
    38101 tcctgacaga cgctagtgac gattactgga atggacttga ggtcgctact ctggtcgacg
    38161 agaagtccaa cctgaaggag ctagccgcta cgcaagcctt gagtctggac cagcagaagg
    38221 gcataaggtt agcgctggaa gtcctgaccg cagcacagca gagagtcgac actggtgata
    38281 cctctggtct gattaagctg attgatgata ataaccttac tgatgactca actataggcg
    38341 actctacgtc aattctgaat aatgagcagg gggatagacc ggcagttttc acacaggagc
    38401 gccagtcatc tgtagttttt tcaagcctag tgtcctcgct gctggctgag aaggtccaga
    38461 ccctgaagac atccagctac aaggacctct cgtcctcact caatactgtc tcccggttcc
    38521 tgcctgaaga catggacctg atgtcccgct ctgggtggct ggctgtcagg gactctatgc
    38581 tggcttctga ggtgagacca tcgacaatca acaagctgct gaccaaagcg aagatgtgtc
    38641 tcgattatgg cctgatgaat gggcaacttg agggccgcaa ccctattgaa cggatgaagc
    38701 tgaccaagga tatcgactcc aagcgcagag cgttcacaga cgaggagctg gagagacttc
    38761 tggtccgtgt tgagt
//
LOCUS       DAZSIO010000019        38604 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZSIO010000019 DAZSIO010000000
VERSION     DAZSIO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.019"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            6..140
                     /locus_tag="ACHX5T_000883"
     CDS             6..140
                     /locus_tag="ACHX5T_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR8466190.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTASGKGKWKIITA
                     "
     gene            119..223
                     /gene="speFL"
                     /locus_tag="ACHX5T_000884"
     CDS             119..223
                     /gene="speFL"
                     /locus_tag="ACHX5T_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR8466191.1"
                     /translation="MENNNRLMPHIRRTTHIMKFTHRNSFDFHFFNAR"
     gene            534..2731
                     /gene="speF"
                     /locus_tag="ACHX5T_000885"
                     /pseudo
     CDS             534..2731
                     /gene="speF"
                     /locus_tag="ACHX5T_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            2728..4047
                     /gene="potE"
                     /locus_tag="ACHX5T_000886"
     CDS             2728..4047
                     /gene="potE"
                     /locus_tag="ACHX5T_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR8466192.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4103..5743)
                     /gene="pgm"
                     /locus_tag="ACHX5T_000887"
     CDS             complement(4103..5743)
                     /gene="pgm"
                     /locus_tag="ACHX5T_000887"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR8466193.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5769..6314)
                     /gene="seqA"
                     /locus_tag="ACHX5T_000888"
     CDS             complement(5769..6314)
                     /gene="seqA"
                     /locus_tag="ACHX5T_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR8466194.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6499..7263
                     /gene="ybfF"
                     /locus_tag="ACHX5T_000889"
     CDS             6499..7263
                     /gene="ybfF"
                     /locus_tag="ACHX5T_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR8466195.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7403..7696
                     /gene="ybfE"
                     /locus_tag="ACHX5T_000890"
     CDS             7403..7696
                     /gene="ybfE"
                     /locus_tag="ACHX5T_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR8466196.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7836..8366
                     /gene="fldA"
                     /locus_tag="ACHX5T_000891"
     CDS             7836..8366
                     /gene="fldA"
                     /locus_tag="ACHX5T_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR8466197.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8576..8662
                     /gene="uof"
                     /locus_tag="ACHX5T_000892"
     CDS             8576..8662
                     /gene="uof"
                     /locus_tag="ACHX5T_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR8466198.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8655..9101
                     /gene="fur"
                     /locus_tag="ACHX5T_000893"
     CDS             8655..9101
                     /gene="fur"
                     /locus_tag="ACHX5T_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR8466199.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9185..9511)
                     /gene="chiQ"
                     /locus_tag="ACHX5T_000894"
     CDS             complement(9185..9511)
                     /gene="chiQ"
                     /locus_tag="ACHX5T_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR8466200.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9548..10954)
                     /gene="chiP"
                     /locus_tag="ACHX5T_000895"
     CDS             complement(9548..10954)
                     /gene="chiP"
                     /locus_tag="ACHX5T_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFR8466201.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(11533..13197)
                     /gene="glnS"
                     /locus_tag="ACHX5T_000896"
     CDS             complement(11533..13197)
                     /gene="glnS"
                     /locus_tag="ACHX5T_000896"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR8466202.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13400..15346)
                     /gene="nagE"
                     /locus_tag="ACHX5T_000897"
     CDS             complement(13400..15346)
                     /gene="nagE"
                     /locus_tag="ACHX5T_000897"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR8466203.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            15679..16479
                     /gene="nagB"
                     /locus_tag="ACHX5T_000898"
     CDS             15679..16479
                     /gene="nagB"
                     /locus_tag="ACHX5T_000898"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR8466204.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            16539..17687
                     /gene="nagA"
                     /locus_tag="ACHX5T_000899"
     CDS             16539..17687
                     /gene="nagA"
                     /locus_tag="ACHX5T_000899"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR8466205.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            17696..18916
                     /gene="nagC"
                     /locus_tag="ACHX5T_000900"
                     /gene_synonym="nagR"
     CDS             17696..18916
                     /gene="nagC"
                     /locus_tag="ACHX5T_000900"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR8466206.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18964..19716
                     /gene="nagD"
                     /locus_tag="ACHX5T_000901"
                     /pseudo
     CDS             18964..19716
                     /gene="nagD"
                     /locus_tag="ACHX5T_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            complement(19767..19865)
                     /locus_tag="ACHX5T_000902"
                     /pseudo
     CDS             complement(19767..19865)
                     /locus_tag="ACHX5T_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20113..21777
                     /gene="asnB"
                     /locus_tag="ACHX5T_000903"
     CDS             20113..21777
                     /gene="asnB"
                     /locus_tag="ACHX5T_000903"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR8466207.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            22156..22232
                     /locus_tag="ACHX5T_000904"
     tRNA            22156..22232
                     /locus_tag="ACHX5T_000904"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22190..22192,aa:Met,seq:cat)
     gene            22241..22325
                     /locus_tag="ACHX5T_000905"
     tRNA            22241..22325
                     /locus_tag="ACHX5T_000905"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22275..22277,aa:Leu,seq:tag)
     gene            22349..22423
                     /locus_tag="ACHX5T_000906"
     tRNA            22349..22423
                     /locus_tag="ACHX5T_000906"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22381..22383,aa:Gln,seq:ttg)
     gene            22458..22532
                     /locus_tag="ACHX5T_000907"
     tRNA            22458..22532
                     /locus_tag="ACHX5T_000907"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22490..22492,aa:Gln,seq:ttg)
     gene            22548..22624
                     /locus_tag="ACHX5T_000908"
     tRNA            22548..22624
                     /locus_tag="ACHX5T_000908"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22582..22584,aa:Met,seq:cat)
     gene            22673..22747
                     /locus_tag="ACHX5T_000909"
     tRNA            22673..22747
                     /locus_tag="ACHX5T_000909"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22705..22707,aa:Gln,seq:ctg)
     gene            22785..22859
                     /locus_tag="ACHX5T_000910"
     tRNA            22785..22859
                     /locus_tag="ACHX5T_000910"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22817..22819,aa:Gln,seq:ctg)
     gene            complement(23013..24188)
                     /gene="ubiF"
                     /locus_tag="ACHX5T_000911"
     CDS             complement(23013..24188)
                     /gene="ubiF"
                     /locus_tag="ACHX5T_000911"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR8466208.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            24334..25758
                     /gene="miaB"
                     /locus_tag="ACHX5T_000912"
     CDS             24334..25758
                     /gene="miaB"
                     /locus_tag="ACHX5T_000912"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR8466209.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            25911..26951
                     /locus_tag="ACHX5T_000913"
     CDS             25911..26951
                     /locus_tag="ACHX5T_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR8466210.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            26948..27415
                     /gene="ybeY"
                     /locus_tag="ACHX5T_000914"
     CDS             26948..27415
                     /gene="ybeY"
                     /locus_tag="ACHX5T_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR8466211.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            27505..28383
                     /gene="corC"
                     /locus_tag="ACHX5T_000915"
     CDS             27505..28383
                     /gene="corC"
                     /locus_tag="ACHX5T_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR8466212.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            28408..29946
                     /gene="lnt"
                     /locus_tag="ACHX5T_000916"
     CDS             28408..29946
                     /gene="lnt"
                     /locus_tag="ACHX5T_000916"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR8466213.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            30343..31251
                     /gene="gltI"
                     /locus_tag="ACHX5T_000917"
     CDS             30343..31251
                     /gene="gltI"
                     /locus_tag="ACHX5T_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR8466214.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            31421..32161
                     /gene="gltJ"
                     /locus_tag="ACHX5T_000918"
     CDS             31421..32161
                     /gene="gltJ"
                     /locus_tag="ACHX5T_000918"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR8466215.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            32161..32835
                     /gene="gltK"
                     /locus_tag="ACHX5T_000919"
     CDS             32161..32835
                     /gene="gltK"
                     /locus_tag="ACHX5T_000919"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR8466216.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            32835..33560
                     /gene="gltL"
                     /locus_tag="ACHX5T_000920"
     CDS             32835..33560
                     /gene="gltL"
                     /locus_tag="ACHX5T_000920"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR8466217.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            33678..34613
                     /gene="rihA"
                     /locus_tag="ACHX5T_000921"
     CDS             33678..34613
                     /gene="rihA"
                     /locus_tag="ACHX5T_000921"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR8466218.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            34697..36367
                     /gene="hscC"
                     /locus_tag="ACHX5T_000922"
                     /pseudo
     CDS             34697..36367
                     /gene="hscC"
                     /locus_tag="ACHX5T_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(36427..37878)
                     /gene="djlC"
                     /locus_tag="ACHX5T_000923"
     CDS             complement(36427..37878)
                     /gene="djlC"
                     /locus_tag="ACHX5T_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR8466219.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(37875..38582)
                     /locus_tag="ACHX5T_000924"
     CDS             complement(37875..38582)
                     /locus_tag="ACHX5T_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR8466220.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         9442 a         9839 c         9758 g         9565 t
ORIGIN      
        1 aattaatgat ttattatctg gggtatgatc gttttttgtt gatcttcttc acagattata
       61 gccatttcat ggatagaata actctacctt caactgacac agcaagcggt aaaggtaaat
      121 ggaaaataat aaccgcttaa tgcctcatat aaggcggaca acccatatca tgaagtttac
      181 ccatcgtaat agttttgact ttcatttctt taatgcccgc tagtcttctg actaaagggc
      241 accccaacgt acaggtctcc ctgactttaa gcattacagg ttaatacctg tattcctcgg
      301 tgctcatata ctgctaaccc ttttaaactc taaataattc gagtcgcagc acttgcaact
      361 tgaggtatga cgagtatagc cagttaccgg gctggtctgg gttattgcat ctgcaaaaag
      421 caaactactg atttatttat cagcggtgga gctttgcttt ttttccgacg tgatcgattt
      481 ctcctttgag aaattgagga cctgctatta cctgaaataa agagatgaat aaaatgtcag
      541 aattaaaaat tgccgttagc cgttcttgcc cggattgttt ttccactcat cgtgcatgcg
      601 tgaatattga cgaaagtaat tatattgacg ttgccgccat tattttatca gtcaatgatg
      661 ttgaacgtgg aaaactcgat aaaatagacg ctactggcta tggcattcct gtttttattg
      721 caacggaaaa tgaagaacgt gtaccagcag aatatctgcc acgtatttct ggtgtcttcg
      781 agcattgcga atcacggaaa gagttttatg gacgtcagtt agaaaccgct gctagccatt
      841 atgaaacgca actgcgcccg cccttcttcc gcgcactggt cgattatgtc aatcagggca
      901 atagcgcttt tgactgcccg ggtcatcagg gaggtgagtt tttccgtcgt catccggcag
      961 ggaatcagtt cgtggagtac ttcggagaaa cgctgttccg ttccgactta tgcaacgcag
     1021 acgtagcaat gggcgatctg ctgattcacg aaggcgcgcc atgcattgca cagcaacatg
     1081 cggcaaaagt gtttaatgcc gataaaacat acttcgttct aaatggcact tcatcttcta
     1141 acaaagtggt tttaaacgcc ctgctaacac cgggtgatct ggtgctgttt gatcgcaata
     1201 accacaaatc taaccaccac ggagcgttgc tacaggctgg tgcaacaccg gtttatctgg
     1261 aaacggcacg taacccgtat ggctttatcg gtggcattga tgcgcactgt tttgaagaaa
     1321 gttacctgcg tgagctgatc gcggaagtcg caccgcagcg ggcaaaagag gctcgtcctt
     1381 tccgcctcgc tgtgattcag ttaggcacct acgacggtac gatttataac gcccgccagg
     1441 tggtggataa aattggtcat ctgtgtgact acattctgtt tgactccgca tgggttggct
     1501 atgagcaatt tatcccgatg atggctgact gttcgccgct gttgctggat cttaatgaga
     1561 acgatccggg tattctagtt acgcaatccg tacataaaca gcaggctggt ttttctcaaa
     1621 cttcacaaat ccataaaaaa gacagccaca tcaaagggca acagcgttat gtaccgcaca
     1681 aacgcatgaa caacgccttt atgatgcacg cctccaccag cccgttctat ccgctgtttg
     1741 ccgcactgga tatcaacgcc aaaatgcatg aaggtgtcag cggtcgtaat atgtggatgg
     1801 attgtgtggt aaatggcatt aatgcccgca aactgatcct cgataactgt cagcatattc
     1861 gtccgttcgt acctgaactg gtggatggta aaccctggca gtcgtatgaa acagcgcaaa
     1921 ttgcggttga tctgcgcttc ttccagtttg taccagggga acactggcat tcttttgaag
     1981 gctatgcaga gaatcaatac tttgtcgatc cttgcaaact gttgctgaca accccaggta
     2041 ttgatgcacg taacggcgaa tatgaagcgt tcggtgtacc cgcgacgatt cttgctaact
     2101 tcctgcgcga aaatggcgta gtgccggaaa aatgcgatct taacgccatc ctcttcctgc
     2161 tgactccggc agaagatatg gccaaacttc agcaacttgt gccctgctgg tacgcttcga
     2221 aaaactgctt gagtccgacg cgccattagc agaagtgctg ccttccatct acaaacagca
     2281 tgaagagcgc tacgccggtt ataccctgcg tcagttgtgc caggaaatgc atgatttgta
     2341 tgcccgccac aacgtgaaac aactgcaaaa agagatgttc cgcaaagcgc acttcccaca
     2401 cgtcagcatg aatccgcaag aagccaacta cgcctattta cgcggtgaag tggagctggt
     2461 tcgtctaccg gatgccgaag gtcgtatcgc tgccgaaggg gcgcttcctt atcctccggg
     2521 tgtgctgtgt gttgttccgg gtgaaatctg gggtggtgct gttttgcgtt acttcagcgc
     2581 tctggaagaa gggatcaacc tgctgccagg ttttgcaccg gagctgcagg gtgtctatat
     2641 cgaagaacat gatggtcgta agcaagtttg gtgctatgtc atcaagcctc gtgatgcgca
     2701 aagcaccctg ttgaaagggg aaaaattatg agtcaggcta aatcgaacaa aatgggcgtc
     2761 gttcagttaa ccatactgac gatggtcaac atgatgggct ccggtatcat catgctgccg
     2821 acaaagcttg ccgaagttgg gacaatttca attatctcct ggctggtgac agccgtaggc
     2881 tcaatggcac tggcatgggc attcgcaaaa tgcggtatgt tcagccgtaa atcaggtggt
     2941 atgggcggtt atgccgagta tgctttcggt aaatccggta actttattgc gaactatacc
     3001 tacggcgtct cactgctgat tgctaacgtc gcgattgcta tttcggcggt tggttacggc
     3061 accgaactgc tcggcgcgag tttgtcgcca gtgcagattg gtcttgcgac catcggggtg
     3121 ctgtggattt gtaccgtggc taactttggt ggtgcgcgca ttaccgggca aatcagtagc
     3181 attaccgtgt gggggggcat tattccggtg gtaggcctgt gcattatcgg ctggttctgg
     3241 tttagcccga cgctttacgt tgattcctgg aatccgcatc atgcaccgtt cttcagtgca
     3301 gtaggttctt ccatcgctat gacgctgtgg gcttttcttg gtctggagtc tgcgtgtgcg
     3361 aatactgatg tagtggaaaa cccggaacgt aatgtgccaa tcgcggtact cggcggtacg
     3421 ttaggtgcgg cggtgattta tatcgtctcc accaacgtga ttgcagggat tgtgcctaat
     3481 atggagctgg caaattcaac ggcaccattt ggcctggcct tcgcacagat gtttacacca
     3541 gaagtgggta aagtcattat ggcgctgatg gtgatgtcct gctgcggttc gctgcttggc
     3601 tggcagttca ccattgccca ggtgtttaaa tcttcatctg atgaaggcta cttccctaaa
     3661 attttctccc gtgtaaccaa agtggatgca ccggtgcagg gaatgttgac cattgtgatt
     3721 atccagagcg ggttggcgct gatgacgatt agcccgtcgc taaacagtca gttcaacgtg
     3781 ctggttaacc tggccgtggt gaccaatatc attccgtata ttctgtcaat ggcggcatta
     3841 gtcattattc agaaggtcgc taatgtgccc ccctcaaaag cgaaagttgc aaactttgtt
     3901 gcttttgttg gcgcgatgta tagcttttat gcgctgtact catccgggga agaagccatg
     3961 ctgtacggtt ccatcgtgac cttccttggt tggacactgt atggtctggt atcaccacgc
     4021 tttgaactga aaaataaaca cggttaaaaa agcaaagtaa aaaagggcga tcttgcgacc
     4081 gctctttttt tattaaatgt gtttacgcgt ttttcagaac ttcgctaaca atctcaaccg
     4141 cttctttctc aatctgcttg cgatgttctt caccgaggaa gctttcgcag tagatcttat
     4201 atgcgtcttc cgtgcctgac ggacgcgcgg cgaaccagcc gttgtcagtc atcactttca
     4261 gaccgccaat agaagcaccg ttgcccggag cagcagtcag gcgcgcggtg atcgggtcac
     4321 ctgccagggt gctggcgctc accatttccg gagacagctt agacagcgcc gctttttgtg
     4381 cggaagtcgc agctgcctgc aaacggttgt agctcggcgc accaaagcgt tttgccagtt
     4441 cgttgtagtg ttcctgcggg ttcttaccgg tgacagcggt gatttccgcc gccagcagac
     4501 acataatgat gccgtctttg tcggtggacc acggcgtgcc gtcgaaacgc aggaaggaag
     4561 cccctgcgct ctcttcgccg ccaaagccga agctgccgtc gaacagacca tcaacaaacc
     4621 atttgaaacc taccggtact tctaccagct tacggcccaa gtcattgacc acacggtcga
     4681 tcatcgcaga agaaaccagc gttttaccga cagcaacatc tttgccccac tgcggacgat
     4741 gctggaacag gtagttgatc gccaccgcca ggtagtggtt cggattcatc aaacctgccg
     4801 gagtgacgat accgtgacgg tcataatccg ggtcgttagc aaacgccaga tcgaacttat
     4861 cacgcagtgc cagcagaccc gccatcgcac actcggagga gcagtccata cggatcgcac
     4921 cgtctttatc gaggtgcata aagcggaagg tttgatcgac ctgatcgtta acgatggtca
     4981 ggttgaggtt gtaatactcg ccaatgcgct tccagtattc gataccggaa ccgcccaacg
     5041 gatcaacgcc cagcgtcagg cccgctttct gaatcgccgc catatcaacg atatcggcca
     5101 gcccttccac gaacggctgc accagatcct gctctttcac atagccggat gccatcgctt
     5161 cgtcgaggga gatacgcttc acgcctttca ggccatcggc catcagtgcg ttggccttgt
     5221 cttccaccac tttggtgacg ttagtatcag ccgggccacc atttggcgga ttgtacttga
     5281 taccaccatc ttccggcggg ttatgggacg gtgtaatcac gataccgtct gccagcgggc
     5341 cacctttttt attgtgaacc aggatggcat tggaaacggc aggcgtcgga gtgaagccat
     5401 tgttttcctg cacaatgaca tcaacgccgt tcgctgccag cacttccagt acggaaatga
     5461 acgcaggctc ggacagggcg tgagtatctt tacccacata gcaagggcca gtgatgccgt
     5521 ttttcgcacg ttcttcagca attgcctgag cgatcgccag aatgtgcggc tcgttaaagc
     5581 tgtggcgcgc tgcactgcca cggtgaccgg aagtaccgaa tttcaccgcg tgctccgcat
     5641 tccctgcttc tggtttcagt acataatatt gcgccgtcag ttgggcgacg ttaatcaaat
     5701 cactctgttg tgcaggttgg cctgcacgat tgtggattgc cattgctttg tcctttgtct
     5761 gcaacgtttt agatagttcc gcaaaccttc tcaatcaatt ccgccgggaa ttgcatcgac
     5821 tgcatgatgt gttcgatcat gctgcattta cggccggtgt tggtgttggt gatcacccaa
     5881 tacggcgtgc ctggcacatg tttcggcttg gtctgattac catttttcag cagcgtttgt
     5941 tcatctgccg caaagtacac gcgtgtacga ccgtgcaacg attccgttgc ttcggcaaac
     6001 gcctgggcgt caagagaata tagtgtagac aacagcagca taaagcgatt gaccgctcgc
     6061 ttttgctctg cgtattcgtc cgaaagcaga agttcacgca ttgcgcgaac cttgtcttta
     6121 atcgttttga ccggcttcgc ttcgacgata gcaggtgacg caacgcgaac ctctttcgtc
     6181 accggagcag caggctgtga tgcggcggaa aatttcaaca tacgccgtaa aatgtcggat
     6241 gcgctctcgc cgatatgctt agtgtggctg gcaatatagc tgtagagttc atcatcaact
     6301 tcaatcgttt tcatcttaat ccagtgcagt gtcttagctg aatacaagtc gccaagagta
     6361 tatgaataaa tcccggcagc ggatagcgtc aagcctggct tgcagaaaaa ctcggaataa
     6421 accttatttg ttgcagccct gtggcacaat ggtcaacata ataccctaac ccgacaacgc
     6481 gaaaaaaaga actttgccat gaaattaaat atccgcgcgc aaactgcaca aaaccagcac
     6541 aataattctc ccatcgttct tgtccatggt ctgtttggta gcctcgacaa ccttggcgta
     6601 ctggctcgcg atctggtaaa cgatcacaat atcatccagg ttgatatgcg taaccacggt
     6661 ctttcaccga gagagccggt aatgaattac ccggcgatgg cgcaggatct tgttgatacc
     6721 ctggatgcac tgcagatcga caaagcaaca tttatcggtc actccatggg cggtaaagcg
     6781 gtaatggcac ttactgcact ggcccccgat cgcatcgata aactggtggc gatcgatatc
     6841 gcaccggtcg actatcacgt acgccgtcat gatgagattt tcgcagctat caacgcggtc
     6901 agtgaatcgg acgcacaaac tcgccagcaa gcagcagcaa taatgcgcca gcatcttaat
     6961 gaagaagggg tgattcagtt tctgttgaaa tcttttgttg acggggagtg gcgctttaac
     7021 gtgccggtat tgtgggatca gtatccgcat attgtgggtt gggagaaaat cccggcatgg
     7081 gatcaccctg ccctgtttat ccctggcggc aattctccgt atgttagcga gcagtaccgt
     7141 gatgatttac tggcacaatt tccacaggca cgggcgcatg tgattgcagg cgcgggtcac
     7201 tgggtccatg ctgaaaaacc ggatgcggta ttacgcgcta tccgtcgcta tctcaatgat
     7261 taacgcaact gattaaaaac ccagcgtccg cgtcgctttc cgcgcgtggg cgttggcgtg
     7321 actcatcgcg ctgatgtatt atggcgcgct atccatccgg gcagaggcct ggctgattgt
     7381 ttcccccgaa gtcaccaaga tcatggccaa agaacaaacg gaccgtacga cattagatct
     7441 gttcgcgcac gagcgtcgac cgggacgacc gaaaactaat ccgctttcgc gcgatgaaca
     7501 gctgcgtatt aataaacgca accagctaaa acgcgacaaa gtacgtggcc ttaagcgtgt
     7561 cgaactgaag ctgaacgcgg aagctgtcga ggcgctgaac gagctggcgg aatcgcgtaa
     7621 tatgagccgt agcgaactga tcgaagagat gttaatgcag caactggcgg cgctgcgtag
     7681 tcagggcatc gtttaaattt ccactttcat gtagcacagt gtgcagtcct gctcgtttgc
     7741 tgattccgca agactgcctg ttctgctatg attgccttta tccgtgggca attttccacc
     7801 cccatttcaa taagtttcaa gaggttattt cactcatggc tatcactggc atctttttcg
     7861 gcagcgacac cggtaatacc gaaaatatcg caaaaatgat tcaaaaacag cttggtaaag
     7921 acgttgccga tgtccatgac attgcaaaaa gcagcaaaga agatctggaa gcttacgaca
     7981 ttctgctgct gggcatccca acctggtatt acggcgaagc gcagtgtgac tgggatgact
     8041 tcttcccgac tctcgaagag attgatttca atggtaaact ggttgcgctg tttggttgtg
     8101 gtgaccagga agattacgcc gaatatttct gcgacgcatt gggcaccatc cgcgacatca
     8161 ttgaaccgcg cggtgcaacc atcgttggtc actggccaac tgcgggctat catttcgaag
     8221 catcaaaagg tctggcagat gacgaccact ttgtcggtct ggctatcgac gaagaccgtc
     8281 agccggaact gaccgctgaa cgtgtagaaa aatgggttaa acagatttct gaagagttgc
     8341 atctcgacga aattcttaat gcctgatgtg atgcggcgta gactcatgtc tacgccgtat
     8401 taatagataa tgccaatcaa aataattgct acaaatttgt aacttttgct gttgtaccta
     8461 tacaatgtcc cagtgttcaa gtggccttgc cgttgtaaat gtaagctgtg ccacgttttt
     8521 attaacaata tttgccaggg acttgtggtt ttcatttagg cgtggcaatt ctataatgat
     8581 acgcattatc tcaagagcaa attctgtcac ttcttctaat gaagtgaacc gcttagtaac
     8641 aggacagatt ccgcatgact gataacaata ccgccctaaa gaaagctggc ctgaaagtaa
     8701 cgcttcctcg tttaaaaatc ctggaagttc ttcaggagcc ggacaaccat cacgtcagtg
     8761 cggaagattt atacaaacgt ctgatcgata tgggtgaaga aattggtctg gctacggtat
     8821 atcgcgtact gaaccagttt gacgacgctg gtatcgtcac ccgccacaat tttgaaggcg
     8881 gtaaatccgt atttgaactg acacagcaac atcaccacga tcacctgatt tgcctcgact
     8941 gcggcaaggt tatcgaattt agtgatgatt ccatcgaagc gcgtcagcgt gaaattgccg
     9001 caaaacatgg cattcgcctg actaaccaca gtctctatct ttacggtcac tgtgccgaag
     9061 gcgattgccg cgaagatgag cacgcgcacg aaggcaaata agccagcctg aacgagaaaa
     9121 gccaacctgc gggttggctt ttttatacaa gggaaataag aaaagcgaga gttacagctc
     9181 tcacttattt gttattactg cgtatttcct gccagacctt atcacaatcg gctttcaccg
     9241 cctgatcatt tccggtttgc gttgcacgca gacactgctg ataatccagt acgcgtacac
     9301 tttcctggtc ggcaaactgc tgatgttgtt tctctttctt cagcacgttt aacacgctct
     9361 ggcaggcttc aattttttct ggcgaacctt gcgcggtgtt gatacaggcg ctgtatgcct
     9421 ctttcagacg gctgtcttcc tgtggtgcgg ttgattgcgc acaagctacc agccccgatg
     9481 ccatcatggc gatgagaatc agttttttca ttgaatgcgg caggcacaaa acctgccgcg
     9541 tcgggcatca gaagatggtg aatggtgcga ttaccataaa tttcacgtca cgctcatcct
     9601 ggaagatgtt accgtaaccg ccgccccagc ttgggatgtc ggagtggttg tcgtattcgg
     9661 tgaagtgcag tttgaacatc gtgcctttgg cgcgaccgtc ctgaatggtg tagaccgcat
     9721 ccaggctgta ggcagactct tcaatagtcc ggtttttgtc gtagtacgca tccggattgc
     9781 tctgccaggt cgcaggttta gcatcccatg cgtaaacgta ggaagcgccg atggcgaagc
     9841 ctggaagatt ccagtttttc aggtcataca tcgcactgaa gaagaccgct ttttcgccgt
     9901 tagcgttgaa gtcggaacgg ttatcccacc agatatccag gcggccgttg gaggaagcgt
     9961 aggttggggt catacgttgc aggaagtatc cctgctgact gtcagcctta atccaggtgc
    10021 cttcgaggcg caaatccact acgtcagccg cccggtaacc aaaggtcaac gcttgcagcc
    10081 aggcggtgcc gtcataaaga tcgttgacgc tgcgatcgtc aactttatcg cgggtaccat
    10141 agaactggta gctggtggtt aacgggctac cggcgatatc aaatttgtag ctggctttgg
    10201 caaaatattg atcgatatac ccttccgcct gaccaaatgc cgcttccagt acgaagttat
    10261 ttttgaagtc gtatttcgca ccgatggagt gcagataatc aactttggtg gttttatcgt
    10321 tctgataaaa ctcatccatt tccagatgcc acggcgcttt gtattcgttg gtccacatgt
    10381 aggagaaact caaggcacca gcatcgccgt aatcaaaatt cgccccggct tccgccccct
    10441 gataagtacc cggcataaag ctccagtgcg gcgctaacag cgtttgaccg gttggctgaa
    10501 tgtaacctgc cctcgcccaa accggaccgt atttaaattt ggccgcagct ttatacaggc
    10561 ttataccgct tttgtcgccg gaccagtctt cgtcataggc tttattactt tttgaaaacg
    10621 cgatttcgtt cgggtgggag ctgtcgccgt tttccgccat ttcaatcgcc gtaaacgcag
    10681 caatatcaag gccgaacata tcagcagcat aaccggactg aaaatcgagg ttggcattcc
    10741 aggtggagtg agaaaggttg gttttgtatt tgtcgccgtc ggtaacatct ttacggtcgc
    10801 gttcacgctg ccagtaatag ataccgccgg ttaaggttga atcgtcgatg aatccttcgg
    10861 cgcgagcctc cggcattgcc ataaagcccg acattgctgt aacaccggca atagccagcg
    10921 ccagcgtact acgtttgcca ctaaacgtac gcatgggtta atcctctttg acgtataaat
    10981 tgctgcacca aaggtgaaca gcgaaaaaaa ataaaaatta aaaaagtagc gttgagaaaa
    11041 gtctcgctga ctacaggaac agactatttt tcgcgacgcg aattatcaat ctctttctgt
    11101 aaccagaatg taagaacatg agcaaccgca catattttga tgatttttgt tacaaggctt
    11161 aagtttgcgg ttgaaaggct tgcagaagaa taaaaacgtg aaattccagc aagatgaaag
    11221 cggttacatt ttatttaatt atgttttgtg attttaaatt tattttatgg ataaattgcg
    11281 aagcggaatt aattcgcgaa gaaaagcaga aaaaacgccg cagagcgcgg cgtttgggat
    11341 tgtttgctat ttcttgccgg atgcggcgtg aacgccttat ccggcctaca aaagcacgca
    11401 aattcaatat attgcagaga tcatgtaggc ctgataagcg aagcgcatca ggcaatttag
    11461 cgtttgaaat ttattgtcta tttattgccg gatgcgacgt aaatgcctca cccggcttgg
    11521 tagaacttaa aattactcgc ctactttcgc ccaggtatca cgcagcccaa cggtgcggtt
    11581 aaataccggt ttttcggccg tggaatggcg gctgtcgagg cagaagtaac cttcacgctc
    11641 aaactggaat gctttacccg caaccgcatc tttcagcgac ggttcagcaa agccctgttt
    11701 gatcaccagc gattccgggt taatcaccga caggaaatca tccgcagcac ctgggttagg
    11761 cacgctgaac agacgatcat acaaacggat ttcaaccggc agcgcatgtg ccgcgctcac
    11821 ccagtgaata acacctttga ctttacgacc atctgccgga tctttgctta aggtgtcggc
    11881 gtcataagta cagaagatgg tggtgatatt accttcggca tctttctcca cgcgttcagc
    11941 cttaatgacg taagcattac gcagacgcac ttctttaccc agcaccagac gtttgtactg
    12001 cttgttagct tcttcgcgga aatcggcgcg atcgatccaa atttcaccgc taaacggcac
    12061 ctgacggctc cccatttccg gtttgttcgg atggttcggc atggtgacca tttcgccttc
    12121 gccctgatag ttttcgataa ccagtttcac cggatcgata accgccattg cgcgcggcgc
    12181 attttcgttg agatcttcac ggatgcagga ttccagcgac gccatctcaa tggtgttgtc
    12241 ctgcttggtc acgccgatgc gtttgcagaa ctcacgaata gaagccgcag tgtaaccacg
    12301 acgacgcaga ccggaaatgg tcggcatacg cgggtcatcc cagccttcaa cgtgcttgtc
    12361 ggtcaccagc aggttcagct tacgcttgga catcacagtg tattccagat tcaggcgcga
    12421 gaattcgtac tggcgcggat gaacaggaat ggtgatgtta tccagtaccc agtcgtacag
    12481 acgacggttg tcctggaact caagcgtaca cagagagtgc gtaatacctt ccagcgcatc
    12541 gctgatgcaa tgggtgaagt cgtacatcgg gtagatgcac cacttgttgc cagtctggtg
    12601 gtgttcagca aatttaatgc ggtacagcac cggatcgcgc attacgataa atggcgacgc
    12661 catgtcgatt ttcgcacgca ggcaggcttt accttcttca aaaccaccgg cacgcatttt
    12721 ttcgaacagc gccaagttct cttcaacgct gcggtcgcgg tacgggctgt ttttacccgg
    12781 ctgcgtcagg gtaccgcggt attcgcggat ctgttccggt gtcagttcat caacgtacgc
    12841 caggccttta ttgatcagtt cgatcgcata ggcgtggagc tgatcaaaat aatcggagga
    12901 gtaacggacg ttaccagacc agtgaaaacc taaccactct acgtcgtttt tgatcgaatc
    12961 gacatactcg atatcttctt ttaccgggtt agtgtcgtcg aaacgcaggt tgcactggcc
    13021 tttatagtcc tgggcgatcc cgaagttcag gcagatagat ttcgcatggc caatatgcag
    13081 atagccattc ggctccggcg ggaaacgggt gtgtactgtg atgtgcttac cactggccag
    13141 atcttcatcg atgatctgac ggataaagtt agtcgggcgg gcttctgcct cactcatcgt
    13201 ggattcctca aagcgtaaac aacgtataac ggcgtatgat cttataagcg ggacaggctg
    13261 acaactgtta gttttttaaa attgataatc taacaaggat tatggggatg attgatgaaa
    13321 aaaaaagcgg cagagaagat ctccaccgct tgtcattgtt ggatgcgacg ctgccgtgtc
    13381 gcatcaggca taaagcagat tactttttga tttcatacag cggtgtttga cccgccacaa
    13441 catggccctg agctttaatg agcaagccac tgaaatcgtc gatattgctg caaaccaccg
    13501 ggctaatcat cgagcgggcg ttagcgttca ggtaatccag atccatttcc agaatcggtt
    13561 gccctgcgct tacctgcgca ccctcttcca ccagacgttt aaagccttta ccttccagcg
    13621 ctacggtgtc gatacccata tggacgacga tctccgcgcc tttttcggtt tccaggcaga
    13681 acgcgtggtt ggtgttgaag attttcacga ttgtccctgc ggctggtgat acgacgattt
    13741 tatctgtcgg tttcaccgcc acaccgtcac ccaccgcttt gctggcgaat gcttcgtcag
    13801 gaacctgatc cagtgccacg acatcaccgg taatcggcga taccagctcc gcgatagata
    13861 ccgcgtttgg tacagcctgc ggttttgcta caggcgcggc agttgccgga gttgcttcag
    13921 ctgacgcagc ggctaccgga ccacgggcaa cgactttctt catcgcatcg ccgatggatt
    13981 ctgctttcgc gccaacaatc acctgaatag tctgtttgtt cagtttcact acaccagaag
    14041 cacccagacg tttacacatc gtatcgttga cgcgggcaga gtcagccacg gtcaggcgca
    14101 gacgggtaat acaggcatca atcgctttca ggttgtcagt accgccaacc gcagcaatat
    14161 agttggttgc cagttgagtc agaccttctt cagtattgct gttggcttct tcagtaacga
    14221 tctcgtcttc tttatcttca cgacccggcg ttttcaggtt gaacatgcgg ataaccaaac
    14281 tgaacaccac gaagtagata gcgaagaaga taacgcccat caccagcagc atccagacgt
    14341 tctggctggc ggcaggcagg ttatacatca acgcgtagtc gatagcaccc gcagagaaag
    14401 agaagccggc atggataccc agcagcgttg ccacaaacag gctgataccg gtcagcagtg
    14461 cgtgcaggag gtacagcagc ggcgcaagga acatgaacag gaattccagc ggctcagtca
    14521 caccggtcag gaacgcagta acagcaacag aaagcagcat cccgccaacc atcggacgac
    14581 gctctttcgg tgctgcgaag tacatcgcca gcgccgcacc cggcagaccg aacatcatga
    14641 tcgggaagaa gccggacatg aacatccccg cggtgccgtc accagcgtag aaacggttga
    14701 tgtcgccgtg gaaaaccgta cccgccgcgt tggtgaattc accaatctgg aaccaggcga
    14761 tggtgttcag cacctgatgc agaccggttg ggatcagcag acggttgatg aaaccaaaga
    14821 taccggaacc cagcgcgccc gcagaaacga tccactcgcc gcctgcatgg atagcgtgct
    14881 gtaccggcgg ccagacgtaa ccaaaaatgg ccgccagcac cagacagaag aagccggtgg
    14941 cgatcggcac aaagcgtttg ccgccgaaga agctcaggaa gtccggcagt ttaatatcgg
    15001 accaacggtt ataggctgcg ccaccaacca gaccggtaat gatacccgcc agtacaccca
    15061 tgttaatttc tgggttgatg gtcaccatcg ctttggttaa cacaaagtaa cctaccgcac
    15121 ccgccagcgc cgccgcacct gcgctgtctt tcgaccagct ggatgccaca ccgatggcga
    15181 agattaatgc gaggttatca aaaattgcac cgcccgcctg ggcaataaac gcaacgttaa
    15241 gtaaatctgg ctgaccgaat cgcagcaaca gcgccgccac cggcagcacc gcgataggga
    15301 gctgtaacgc cctaccgagt cgctggaaaa aacctaaaat attcatctta ttccccctac
    15361 gagaacccta tttggctcgt ttaaagccgt atttttattt tgctgcaaat tttactgccg
    15421 atgttctgta atcagattgt tagatcatct gctacagagt gtgtgaaaat ttaattcgca
    15481 tcgcaaatta aacgcgtgtc ttttgtgagt tttgtcacca aatatcgtta tcatcactcc
    15541 cttttactgg ctaaaccaga aaacttattt tatcattcaa aaaatcaagt cggattgacg
    15601 cctgtctgcg caaatccagg ttacgcttaa agatgtctaa tccgccaacg gcttacattt
    15661 tacttattga ggtgaataat gagactgatc cccctgacta ccgctgaaca ggtcggcaaa
    15721 tgggctgctc gccatatcgt caatcgtatc aatgcgttca aaccaactgc cgatcgtccg
    15781 tttgtactgg gcctgccgac tggcggcacg ccgatgacca cctataaagc gttagtcgaa
    15841 atgcataaag caggccaggt cagctttaag cacgttgtca ccttcaacat ggacgaatat
    15901 gtcggtctgc cgaaagagca tccggaaagc tactacagct ttatgcaccg taatttcttc
    15961 gatcacgttg atattccagc agaaaacatc aaccttctca acggcaatgc cccggatatc
    16021 gatgccgagt gccgccagta tgaagaaaaa atccgttctt acggaaaaat tcatctgttt
    16081 atgggcggtg taggtaacga cggtcatatt gcatttaacg aaccggcgtc ttctctggct
    16141 tctcgtactc gtatcaaaac cctgactcat gacactcgcg tcgcgaactc tcgtttcttt
    16201 gataacgatg tcaatcaggt gccaaaatat gccctgactg tcggtgttgg tacactgctg
    16261 gatgccgaag aagtgatgat tatggtgctg ggtagccaga aagcactggc gctgcaggcc
    16321 gccgttgaag gttgcgtgaa ccatatgtgg accatcagct gtctgcaact gcatccgaaa
    16381 gcgatcatgg tgtgcgatga accttccacc atggagctga aagttaagac tttaagatat
    16441 ttcaatgaat tagaagcaga aaatatcaaa ggtctgtaat tgttatccct gccctggttc
    16501 cttgctcagg gcaatatttt ttaaaatcgg ggggcagaat gtatgcatta acccagggcc
    16561 ggatctttac cggccacgaa tttcttgatg accacgcggt tgttatcgct gatggcctga
    16621 ttaaaagcgt ctgtccggta gcggaactgc cgccagagat cgaacaacgt tcactgaacg
    16681 gggccattct ctcccccggt tttatcgatg tgcagttaaa cggctgcggc ggcgtacagt
    16741 ttaacgacac cgctgaagcg gtcagcgtgg aaacgctgga aatcatgcag aaagccaatg
    16801 agaaatcagg ctgtactaac tatctgccga cgcttatcac caccagcgat gagctgatga
    16861 aacagggcgt gcgcgttatg cgtgagtacc tggcaaaaca tccgaatcag gcgttaggtc
    16921 tgcatctgga aggtccgtgg ctgaatctgg taaaaaaagg cacccataat ccgaattttg
    16981 tgcgtaagcc tgatgccgcg ctggtcgatt tcctgtgtga aaacgccgac gtcattacca
    17041 aagtgaccct ggcaccggaa atggttcctg cggaagtcat cagcaaactg gcaaatgccg
    17101 ggattgtggt ttctgccggt cactccaacg cgacgttgaa agaagcaaaa gccggtttcc
    17161 gcgcggggat tacctttgcc acccatctgt acaacgcgat gccgtatatt accggtcgtg
    17221 aaccgggcct ggcgggcgcg atcctcgacg aagctgacat ttattgcggt attatcgctg
    17281 atggcctgca tgttgattac gccaacattc gtaacgctaa acgccttaaa ggcgacaaac
    17341 tgtgtctggt taccgacgcc accgcgccag caggtgccaa cattgaacag ttcatttttg
    17401 cgggtaaaac aatatactac cgtaacggac tttgtgtgga tgagaacggt acgttaagcg
    17461 gttcatcctt aaccatgatt gaaggcgtgc gtaatctggt cgaacattgc ggtatcgcac
    17521 tggatgaagt gctgcgtatg gcgacgctct atccggcgcg tgcgattgac gttgaaaaac
    17581 gtctcggcac gctcgccgca ggtaaagtag ccaacctgac cgcattcaca cctgatttta
    17641 aaatcaccaa gaccatcgtt aacggtaacg aggtcgtaac tcaataagag aaagtatgac
    17701 accaggcgga caagctcaga taggtaatgt tgatctcgta aaacagctta acagcgcggc
    17761 ggtttatcgc ctgattgacc agtacgggcc aatctcgcgg attcagattg ccgagcaaag
    17821 ccagcttgcc cccgccagcg taaccaaaat tacgcgtcag cttatcgaac gcgggctgat
    17881 caaagaagtt gatcagcagg cctccaccgg gggccgccgc gctatctcca tcgtcaccga
    17941 aacccgcaat ttccacgcaa tcggcgtacg gcttggtcgt catgacgcca ccatcactct
    18001 gtttgatctc agcagcaaag tgctggcaga agaacattac ccgctgccgg aacgtaccca
    18061 gcagacgctg gaacatgccc tgctgaatgc cattgctcag tttattgata gctaccagcg
    18121 caagctgcgc gagctaatcg ccatttcggt gatcctgcca gggcttgttg acccggacag
    18181 cggcaaaatt cattacatgc cgcatattca ggtagaaaac tgggggctgg tagaagcact
    18241 ggaagaacgt tttaaagtga cctgtttcgt tggtcacgat atccgtagtc tggcgctggc
    18301 ggagcactac ttcggtgcaa gtcaggattg cgaagactcc attctggtgc gtgtccatcg
    18361 cggaaccggg gccgggatta tctctaacgg gcgcattttt attggccgca acggtaacgt
    18421 cggtgaaatt ggccatattc aggtcgaacc gctgggtgaa cgctgccact gcggcaactt
    18481 tggctgcctg gaaactatcg ccgccaacgc tgccattgaa caacgggtgt tgaatctgtt
    18541 aaagcagggc taccagagcc gtgtgccact ggacgactgc accatcaaaa ctatctgcaa
    18601 agccgcgaac aaaggcgata gcctggcctc ggaagtgatt gagtatgtcg gtcgtcatct
    18661 gggtaaaacc atcgccattg ctatcaactt atttaatccg caaaaaattg ttattgctgg
    18721 tgaaatcacc gaagccgata aagtgctgct ccctgctatt gaaagctgca ttaataccca
    18781 agcgctgaag gcgtttcgta ctaatctgcc ggtggtacgt tctgagctgg atcaccgctc
    18841 ggcaatcggc gcttttgcgc tggtaaaacg cgccatgctc aacggtattt tgctccagca
    18901 tttgctggaa aattaatgtg cttttatagt ggcgcttatt gttgtcaata ttctgggtag
    18961 tccatgacca ttaaaaatgt aatttgcgat atcgacggcg tgctgatgca cgataacgtc
    19021 gccgtaccgg gtgcagcgga atttttgcac gggattatgg ataaaggcct gccgctggtg
    19081 ttgctgacca actatccttc gcagactggg caagatctgg cgaaccgctt tgccactgcc
    19141 ggtgtcgatg tgccggatag cgtgttttat acctctgcga tggcgaccgc cgatttcctg
    19201 cgtcgctagg aaggcaagaa agcgtatgtg gtgggcgaag gcgcactgat tcatgaactg
    19261 tacaaagccg gtttcactat taccgatgtg aaccctgatt tcgtgattgt tggcgaaaca
    19321 cgttcctaca actgggacat gattcataaa gcagcctatt tcgtcgctaa cggtgcacgc
    19381 tttatcgcca ccaacccgga cacccacggg cgcggttttt atcccgcttg tggcgcgttg
    19441 tgtgcaggga ttgagaaaat ctccgggcgc aaaccgttct atgttggtaa gcccagcccg
    19501 tggatcatcc gcgcggcatt aaacaaaatg caggcgcatt cggaagaaac agtgattgtc
    19561 ggcgataacc tgcgtaccga tattctggcg ggcttccagg ccggtctgga gacgattctg
    19621 gtgctttctg gtgtttcatc gctcgacgat atcgacagta tgccgttccg cccaagctgg
    19681 atttacccgt cagtcgctga aatcgacgtt atctgaatac cacccacgtc tgcggacgtg
    19741 gttggctctg atgacaaacg ccaaactgcc tgatgcgcta cgcttatcag gcctacgcag
    19801 ctcctgcaat atattgaatt ttcatgcttt tgtaggccgg ataaggcgtt cacgccgcat
    19861 ccggcatgaa caaatcgcac tttatcagca atttggcccc cgtaaaatat aaccattcaa
    19921 taaaacccgc taaaaattat cgattcatca ataaatacat cctcacacca gacccttttt
    19981 caccaatcga caatcaccat tacgtttttt atttttgcac tgccaaatag cttgcgcaca
    20041 gtgctgcttt gcggcatttt tttaaacaag caaacacaac aagcaacaaa taccaggtta
    20101 acggagaagg ttatgtgttc aatttttggc gtattcgata tcaaaacaga cgcagttgag
    20161 ctgcgtaaga aagcactcga gctgtcacgc ctgatgcgtc atcgtggccc ggactggtcc
    20221 ggtatttatg ccagcgataa cgccattctc gctcacgaac gtctgtcaat tgttgacgtt
    20281 aacgcggggg cgcaacctct ctacaaccaa caaaaaaacc acgtactggc ggtaaacggt
    20341 gaaatctaca accaccaggc attgcgcgct gaatatggcg atcgttatca gttccagaca
    20401 gggtctgact gtgaagtgat cctcgcgctg tatcaggaaa aagggccgga atttctcgac
    20461 gacttgcagg gcatgtttgc ctttgccctg tacgacagcg aaaaagatgc ctacctgatt
    20521 ggtcgcgacc atctggggat catcccactg tatatggggt atgacgaaca cggtcagctg
    20581 tatgtggcct cagaaatgaa atcgctggtg ccagtttgcc gcacgattaa agagttcccg
    20641 gcggggagct atttgtggag tcaggacggc gaaatccgtt cttattatca tcgcgactgg
    20701 ttcgactttg atgcagtgaa agataacgtg accgacaaaa acgagctgcg tcaggcactg
    20761 gaagattccg ttaaaagcca tctgatgtct gatgtgcctt acggtgtgct actttctggt
    20821 ggtctggatt cctcaattat ttccgctatc accaagaaat acgcagcccg tcgcgtggaa
    20881 gatcaggaac gctctgaagc ctggtggccg cagttacact cctttgctgt aggtctgccg
    20941 ggttcaccgg atcttaaagc agcccaggaa gtggcaaacc atctgggcac ggtgcatcac
    21001 gaaattcact tcactgtaca ggaaggtctg gatgccatcc gcgacgtgat ttaccacatc
    21061 gaaacttatg atgtgaccac tattcgcgct tcaacaccga tgtatttaat gtcgcgtaag
    21121 atcaaggcga tgggcattaa aatggtgctg tccggtgaag gttctgatga agtgtttggc
    21181 ggttatcttt acttccataa agcgcccaac gttaaagaac tgcatgaaga gacggtgcgt
    21241 aaactgctgg ccttgcatat gtatgactgc gcgcgcgcca acaaagcgat gtcagcctgg
    21301 ggcgtggaag cacgcgttcc gttcctcgac aaaaaattcc ttgatgtggc gatgcgcatt
    21361 aacccgcagg ataaaatgtg cggtaacggc aaaatggaaa aacacatcct gcgtgaatgt
    21421 tttgagtcat acctgcccgc aagcgtggcc tggcggcaga aagagcagtt ctccgatggc
    21481 gtcggttaca gttggatcga caccctgaaa gaagtggcgg cgcagcaggt ttctgatcag
    21541 caactggaaa ctgcccgctt ccgcttcccg tacaacacgc cgacctcaaa agaagcgtat
    21601 ctgtaccgcg agatctttga agaactgttc ccacttccga gtgccgctga gtgcgtgccg
    21661 ggcggtcctt ccgtcgcgtg ttcttcagct aaagcgatcg aatgggatga agcgttcaaa
    21721 aaaatggacg atccgtctgg tcgtgcggtt ggcgttcacc agtcggcgta taagtaagaa
    21781 atgcggtact gccccggaga aatccggggc tgttttagtt attgcgctgc ttgttgattc
    21841 tgatatgcag taaggaaatg ttccgggttt gccacatcac tgtttgacaa ctcatcactg
    21901 taggccagta tttcatagat taatttccca ccactattgt tgaattccga atttcatcct
    21961 gtacgacaaa agaatggctg tagggtgggg aaaaacacaa agtaaacaat aattgacgaa
    22021 tatagcgcca cgctgttcgc aacctaacca aacagtcact ttcgagcaat tttccttgaa
    22081 aaagaggttg acgctgcaag gctctatacg cataatgcgc cccgcaacgc cgataaggta
    22141 tcgcgaaaaa aagatggcta cgtagctcag ttggttagag cacatcactc ataatgatgg
    22201 ggtcacaggt tcgaatcccg tcgtagccac catctttttt gcgggagtgg cgaaattggt
    22261 agacgcacca gatttaggtt ctggcgccgc aaggtgtgcg agttcaagtc tcgcctcccg
    22321 caccattcac cagaaagcgt tgtacggatg gggtatcgcc aagcggtaag gcaccggttt
    22381 ttgataccgg cattccctgg ttcgaatcca ggtaccccag ccatcttctt cgagtaagcg
    22441 gttcaccgcc cggttattgg ggtatcgcca agcggtaagg caccggtttt tgataccggc
    22501 attccctggt tcgaatccag gtaccccagc catcgaagaa ataatctggc tacgtagctc
    22561 agttggttag agcacatcac tcataatgat ggggtcacag gttcgaatcc cgtcgtagcc
    22621 accaaattct gaatgtgtcg aatacgttcg gcaaattcaa aaaccaattt gttggggtat
    22681 cgccaagcgg taaggcaccg gattctgatt ccggcattcc gaggttcgaa tcctcgtacc
    22741 ccagccaatt tattcaagac gcttacatcg taagtgcacc cagttggggt atcgccaagc
    22801 ggtaaggcac cggattctga ttccggcatt ccgaggttcg aatcctcgta ccccagccac
    22861 attaaaaaag ctcgcttcgg cgagcttttt gcttttctgc gttcattcaa tgtcgaatgc
    22921 gatgttgaca cgtcttatcc ttcaatgtcg gatgcgacgc tgccgcgtct tatccgacct
    22981 acggctggca cgcatccggc aatgttgtaa ggctacaacc ctaacgcata tttcagcgcc
    23041 tgacgtttca acacgccagc acgctctgcc gccattaacc cgagattacg cacaaaacgc
    23101 agcggcggca gattattact gaatccggca taaaacagat ccataccact ttgcataatg
    23161 aagttatccg ccatgcgccg catctggtaa cgcttgagga caggataact ggcccacgct
    23221 tcaccgtagc tgcgggcatt caccagaaca tcaatcaggg catcgacatc acgataacca
    23281 agattcaccc cctgccccgc cagcggatgg atggtatgcg cggcatcgcc caccagcgca
    23341 agccccggct gcacatactg caacgcatgt cggcgcgtca gcggaaacgc accagcggca
    23401 agcggtgtaa cgtaacccag acgcgacggg aaatgcttcg cgatttccgc ctggagttgc
    23461 gccatattca tattctgcaa ctggcgaata cgcgtcggag agtcatacca caccagcgat
    23521 gcccagttat caaacaacgg cagaaacgca cgcggtccgt ccggggtaaa ttgctgccag
    23581 gtgctgtcgc cgggatcgtt ctcacactgt acgctaatca acatgcacga ctgcgcatac
    23641 tgccaggcat gaacgccaat tcccgccatc tgccgcacct gagaatttgc gccgtcggca
    23701 ccaatcacca gtttcgcgcg gatcgtttca ccgcccttca gttccagctc ctgaagatca
    23761 ttatctcgat gtaatgcaat cagcgaggtt ggcacacgta acgttacttt cggatgcgct
    23821 tccagcgcct gccacaacgc ctgttgcagc actgtgtttt ccaccatgta gccaagcaac
    23881 ggcaacttta attcggctgc gtcaaatacc acatgtgccg tttcccactc ccaggtttcc
    23941 agtctgcggt aaggatggca acgcatagcc tgtactgcat cccagacccc taacccttta
    24001 agcaatgata ccgaagccgc gctgatcgcc gagatccgca cgtccggttg gctgtcagcg
    24061 acaaacggtg caggttctgc gtgctcgatc accgttaccg aaaatccgtg ctgtgccagc
    24121 cccagcgcca gtgcgccgcc gaccattcct ccgccgacaa tggcaatttc cgttggttga
    24181 tttgtcatgg tcaatcatcc tgttagcaat atgctttaag tttacaggat ttttaccccc
    24241 aagggctgat aacgctcata ctggtcacaa cggcagcaaa gcattacact atgcgccctg
    24301 cattcctggc tactatttcg caagagcaag tcgatgacca aaaaactcca tattaaaacc
    24361 tggggctgtc agatgaacga gtacgattca tcgaagatgg ccgatctgct ggatgccacc
    24421 cacggctatc aactgaccga cgtggcggaa gaagcggatg tgctactgct gaacacctgc
    24481 tcaatccgcg agaaggctca ggaaaaagtc ttccatcagt tgggtcgctg gaaactgtta
    24541 aaagagaaga atccagacct gattatcggc gtcggtggct gcgtggcatc gcaagaaggc
    24601 gagcacattc gccagcgcgc ccactatgtc gatattattt ttgggccgca aacgctgcac
    24661 cgtctgccgg agatgatcaa ctccgtgcgc ggcgaccgca gcccggttgt agatatcagc
    24721 ttcccggaaa tcgagaagtt tgaccgtctg ccggaaccgc gcgccgaagg gccgaccgcg
    24781 tttgtctcca tcatggaagg ctgcaataaa tattgcacct actgcgtggt gccttacacc
    24841 cgtggtgaag aggtaagccg tccgtctgac gatattctgt ttgagattgc ccagcttgcg
    24901 gctcagggcg tgcgtgaagt caacctgctc ggtcagaatg tgaatgcctg gcgtggtgag
    24961 aactacgacg gcaccaccgg atcgtttgcc gatctgttgc gtctggttgc tgcgatcgac
    25021 gggatcgatc gtattcgctt taccaccagc catccgatcg aattcaccga cgatatcatc
    25081 gaagtgtacc gcgacacgcc tgagctggtg agcttcctgc atctgccggt acagagcggt
    25141 tccgatcgca ttctgaacct gatgggacgt acccatacgg cgctggagta caaagcgatc
    25201 atccgtaaac tgcgtgcggc gcgtccggat attcagatca gttctgactt cattgttggc
    25261 ttccctggcg aaaccaccga agacttcgag aaaacgatga agctgattgc cgacgtcaat
    25321 ttcgacatga gctacagctt tatcttctct gcacgtccag gaacgcctgc cgccgatatg
    25381 gttgatgatg ttccggaaga agagaagaag cagcgtctgt atattctgca agaacgtatc
    25441 aatcagcaag cgatggcatg gagccgtcgg atgctcggca ccacccagcg tattctggta
    25501 gaagggactt cgcgtaagag catcatggag ctttccgggc gtaccgaaaa taaccgcgtg
    25561 gtcaacttcg agggcacacc ggatatgatc ggtaaattcg tcgatgtaga aattaccgac
    25621 gtctacccga actctctgcg cggtaaagtg gtgcgaactg aagatgaaat gggtctgcgc
    25681 atggcagaaa caccggagtc ggtgattgcc cgcacccgca aagaaaacga ccttggcgtg
    25741 ggttattatc agccgtaatt ctcaggcctg ccgttccggt gggccttgtt ttttctctct
    25801 ttatccccat ctttatcgca atgcttgcgc ctttttcctg tggcgtgaat aatttcctta
    25861 taggccggtg aatgttcagc ttccggcggc atacagataa gaggaacggt ttgaacatag
    25921 acactcgcga aatcaccctg gagccagcag acaatgcgcg tctgttgagc ctgtgcggcc
    25981 cgtttgatga caacatcaag cagctcgaac gccgtctcgg catcgagatc aatcgccgcg
    26041 ataaccactt taaactgacc ggccgtccga tttgcgtcac cgctgcggca gacattctgc
    26101 gtagcctgta tgtcgatact gccccgatgc gcggtcagat tcaggatatc gaaccggaac
    26161 agatccacct tgcgattaaa gaagcgcggg tactggagca aagcgcggag agcgtgccgg
    26221 agtacggcaa agcggtcaat atcaaaacta aacgcggcgt aattaagccg cgcacgccaa
    26281 accaggcgca gtacatcgcc aatattctcg accatgacat taccttcggc gttggcccgg
    26341 cgggtacggg taaaacctac ctggcagtgg ctgcggcagt tgatgccctg gagcgtcagg
    26401 aaattcgccg tattctgctg actcgtccgg cggtcgaagc cggtgagaaa ctgggcttcc
    26461 tgcctggcga tttaagccag aaagtagacc cgtatctgcg cccactgtac gacgcgctgt
    26521 ttgaaatgct gggctttgag aaagtcgaga aactgattga gcgcaacgtt attgaagtcg
    26581 cgccgctggc ctatatgcgt ggtcgtacgc tgaacgacgc gtttatcatt ctcgatgaga
    26641 gccagaacac taccatcgaa cagatgaaga tgttcctgac ccgtatcggt tttaactcaa
    26701 aagcggttat caccggcgac gtcacacaga tcgacctgcc gcgtaatact aaatcaggct
    26761 tacgtcatgc cattgaagtg ctggccgatg tcgaagagat cagctttaac ttcttccaca
    26821 gcgaagacgt ggttcgtcac ccggtggtgg cgcgtatcgt taacgcttat gaagcctggg
    26881 aagaagcaga acaaaaacgt aaagcggcgc tggcagcaga acgcaagcgc gaagaacagg
    26941 aacaaaaatg agtcaggtga tcctcgattt acaactggca tgtgaagata attccgggtt
    27001 accggaagag agccagtttc aaacatggct gaatgcggtg atcccgcagt ttcaggaaga
    27061 gtcggaagtg actattcgcg tggtcgatac cgccgaaagc cacagtctga atctgaccta
    27121 tcgcggtaag gataagccga ccaacgtgct ctccttcccg tttgaagtgc cgcctggcat
    27181 ggagatgtcg ctactgggcg atctggttat ctgccgtcag gtggttgaga aggaagctca
    27241 ggagcaaggc aaaccactgg aggcgcactg ggcgcatatg gtggtgcacg gcagtctgca
    27301 tttgttaggt tacgatcaca ttgaagatga cgaagcagaa gaaatggaag ccctcgaaac
    27361 agagattatg cttgctctgg gctatgagga tccgtacatt gccgagaaag aataatttgc
    27421 caggcatttg actggcagac gtccccgccg ttggtgatta acgacggcgt tttgattaac
    27481 taacatgaga acccatacga cgccatgagc gacgacaatt cacacagtag tgacacgata
    27541 agcaacaaga agggattttt ctccctgtta ctcagccaac ttttccacgg tgaaccgaaa
    27601 aaccgtgacg aactgctggc gctgatccgt gattccgggc agaacgacct tatcgacgaa
    27661 gatacgcgcg atatgctcga aggggtgatg gacatcgcag accaacgcgt tcgcgacatc
    27721 atgatccccc gctcccagat gattaccctg aaacgcaacc agacgctgga cgaatgcctt
    27781 gatgtcatca tcgagtccgc ccactcacgt ttcccggtga ttagcgaaga caaagatcac
    27841 attgaaggga ttctgatggc gaaagacttg ctgccgttta tgcgcagcga tgctgaagcc
    27901 ttcagcatgg acaaagtgtt acgtcaggcg gttgtcgttc ctgaaagtaa gcgcgtagac
    27961 cggatgctga aagagtttcg ctctcagcgt taccacatgg cgatcgttat tgacgaattc
    28021 ggtggggttt ccggtctggt gaccattgaa gacatcctgg aactgattgt tggtgagatt
    28081 gaagacgagt atgacgaaga agatgatatc gacttccgtc agctgagtcg tcatacctgg
    28141 accgtgcgcg cactggcttc cattgaagac ttcaacgaag cgttcggcac ccactttagc
    28201 gatgaagaag tcgacactat cggtggtctg gtgatgcagg cgtttgggca tcttccggcg
    28261 cgtggcgaaa ctatcgacat cgacggttac cagttcaaag tggcgatggc cgatagtcgg
    28321 cgtattattc aggttcatgt caaaatcccg gatgactcac cccagccaaa gctggatgaa
    28381 taaaaccgaa actggataga taactacatg gattttgcct cattaattga acgccagcgc
    28441 attcgcctgc tgctggcgtt attattcggt gcctgcggaa cgctggcctt ctctccttac
    28501 gacgtctggc ctgcggcgat tatttcgctg atggggcttc aggcgttgac ctttaaccgt
    28561 cgcccactcc agtctgccgc tattggcttt tgctggggat ttggcctctt tggcagcggt
    28621 attaactggg tctatgtcag catcgcgacc tttggcggga tgcctggccc ggttaacatc
    28681 ttcctggtgg tgctgctggc ggcgtatttg tcgctgtata ccggactgtt tgctggcgtg
    28741 ctgtcgcgtc tgtggccgaa aaccacctgg ctgcgcgtgg cgattgccac ccctgctctc
    28801 tggcaagtga ccgagtttct gcgcggttgg gtgctgactg gcttcccgtg gttacagttc
    28861 ggctatagcc agattgatgg cccgttaaaa gggctggcac cgctaatggg cgtggaagcc
    28921 attaacttcc tgctgatgat ggttagtggc ctgctggcac tggcgttggt caaacgcaac
    28981 tggcgtccgc tggtggtggc cgtcgtgctg tttgccctac ccttcccgct gcgttacatc
    29041 caatggttta ccccgcaacc ggagaaaacc attcaggttt cgatggttca gggcgatatt
    29101 ccgcaatcgc tgaaatggga cgaaggccag cttcttaata cgctaaagat ttactacaac
    29161 gcaacggcac cgctgatggg caaatcatcg ttgattatct ggccggagtc ggcgataacc
    29221 gatctggaaa ttaatcagca accgttcctc aaagcactgg acggtgagtt gcgtgataaa
    29281 ggtagctcgc tggtaaccgg gattgtcgac gcgcgtctca ataagcagaa ccgctacgat
    29341 acctacaaca ccatcatcac gctgggtaaa ggtgcaccgt acagctacga atcagccgat
    29401 cgctataaca aaaaccatct ggtgccgttt ggcgagtttg tcccgctgga gtcgattctg
    29461 cgtccgttag caccgttctt tgatctgccg atgtcgtcgt tcagccgtgg gccatatatc
    29521 cagccgccgc tgtcggcaaa tggtattgag cttactgcgg ctatttgcta cgagattatt
    29581 ctcgccgagc aagtgcgcga taacttccgc ccggataccg actacctgct gactatctcc
    29641 aacgatgcgt ggtttggtaa atctattggt ccatggcaac acttccagat ggcgcgaatg
    29701 cgtgcgctgg agctggcgcg cccactgttg cgcagcacca acaacggcat tacggcggtg
    29761 attggcccgc agggtgagat tcaggcgatg atcccgcagt tcgcccgcga ggtgttaacc
    29821 actaacgtga cgccgaccac cggactcaca ccatacgcac gtaccggcaa ctggccgctg
    29881 tgggtgctga cggcactgtt tggttttgct gctgtgttga tgagtctgcg tcagcgacgt
    29941 aaataatccc ttcttatcgt gccggaatac atcccatatt tcggcacgct caccggatgc
    30001 ggcatcagtg cattatccgg ataacaatat tctcctatcc aactttggca catctattgc
    30061 tttgttatac aaggcaaagc ttgaaccagc atcagtacaa cccggttgca ccacagcaat
    30121 ccaccggtag caccaaaaga gtgcatttta agaattttgc ttcagaatgg tgcggtgcac
    30181 ctcgcaaaaa caaacaataa aacatcaata tctttacatt tagccaaact aaatgttacc
    30241 taacaggcac aacactgcac aataaagttg cagacgataa caacacaaac actcacaacg
    30301 ggtatccatg cgttcttaac gcagaagata aaggtgttgg atatgcaatt acgtaaacct
    30361 gccacagcaa tcctcgccct ggcgctttcc gcaggactgg cacaggcaga tgacgccgcc
    30421 ccggcagcag gcagtacgct ggacaaaatc gccaaaaacg gtgtgattgt cgtcggtcac
    30481 cgtgaatctt cagtaccttt ctcttattac gacaatcagc aaaaagtggt gggttactcg
    30541 caggattact ccaacgccat tgttgaagca gtgaaaaaga aactcaacaa accggacttg
    30601 caggtaaaac tgattccgat tacctcacaa aatcgtattc cactgctgca aaacggcact
    30661 ttcgattttg aatgtggttc taccaccaac aacgtcgaac gccaaaaaca ggcggctttc
    30721 tctgacacta ttttcgtggt cggtacgcgc ctgttgacca aaaagggcgg cgatatcaaa
    30781 gattttgccg acctgaaagg caaagccgta gtcgtcactt ccggcactac ctctgaagtt
    30841 ttgctcaaca aactgaatga agagcaaaaa atgaatatgc gcatcatcag cgccaaagat
    30901 cacggtgact ctttccgcac cctggaaagc ggtcgtgccg ttgcctttat gatggatgac
    30961 gctctgctgg ccggtgaacg tgcgaaagcg aagaaaccag acaactggga tatcgtcggc
    31021 aagccgcagt ctcaggaagc ctacggttgc atgttgcgta aagacgatcc gcagttcaaa
    31081 aagctgatgg atgacactat cgctcaggtg cagacctccg gtgaagcgga aaaatggttt
    31141 gataaatggt tcaaaaatcc aattccgccg aaaaacctga acatgaattt cgaactgtca
    31201 gacgaaatga aagcactgtt caaagaaccg aatgacaagg cactgaacta attacaagaa
    31261 ccaggggcgg aaattccagc cctctcgatt gttacgtagc acggacagac tatacgcctg
    31321 atggtcgttc cccatcgggc ctgaaaaccg caatacgctg ggtaacaatc ttcgagggta
    31381 gcagttaacg ctgctaccct cttttttctg gagtagattt atgtctatag actggaactg
    31441 gggtattttt ttacaacaag ccccgttcgg caataccacc tatcttggtt gggtctggag
    31501 tggttttcag gtgacgatcg ctttgtcgat ctgcgcctgg attatcgctt tcctcgtcgg
    31561 ttcatttttc ggcattttac gtaccgttcc aaaccgtttt ctctccggtc tgggtacgtt
    31621 gtatgtcgaa ctgttccgca acgtgccgtt gattgtgcaa ttctttacct ggtatctggt
    31681 gatcccggag ctgctgccgg agaaaatcgg catgtggttt aaggccgagc tggatcccaa
    31741 tattcagttt ttcctttcat ccatgctctg cctggggctg tttaccgccg cccgtgtttg
    31801 cgaacaggta cgcgccgcta ttcagtcgct accgcgtggg cagaagaatg ccgcgctggc
    31861 gatgggcctg acgctaccgc agacttatcg atacgtactg ctgcctaatg cttatcgcgt
    31921 tatcgtcccg ccgatgactt ccgagatgat gaacctggtg aaaaactccg ccatcgcctc
    31981 gaccatcggt ctggtggata tggcggcgca ggcgggtaaa ttgctcgact actcggctca
    32041 cgcctgggag tcgtttaccg ccatcacgct tgcgtatgtg ttgattaacg cttttatcat
    32101 gctggtgatg acgctggttg aacgtaaagt tcgcctgccc ggcaacatgg ggggcaaata
    32161 atgtacgagt ttgactggag ttccattgtt ccttccctgc catatctgct cgacgggctg
    32221 gtgatcaccc tgaaaatcac cgtcacggca gtcgtgattg gtattttgtg gggtaccatg
    32281 ctggcggtga tgcgcttgtc cagcttcgcg cctgtttcgt ggtttgccaa agcctacgtt
    32341 aacgtattcc gctcgattcc tttagtcatg gttttgctgt ggttttactt gattgtgccg
    32401 ggatttctgc aaaatgtgct gggattatcg ccaaaaaatg atattcgttt aatttcggcg
    32461 atggtggcgt tttcgatgtt tgaagcggcc tactattcag agattatccg tgccggtatt
    32521 caaagtattt cccgtggtca gtcgagcgcc gcgctggcgt tggggatgac tcactggcag
    32581 tcgatgaaac tgattattct gccgcaggct ttccgcgcga tggtgccgct gctgctcact
    32641 cagggcatcg tactgttcca ggatacctcg ctggtgtatg tgttatctct ggccgatttc
    32701 ttccgtaccg cctcaaccat tggtgagcgt gatggtacgc aggtcgagat gatcctgttt
    32761 gccggatttg tttatttcgt tattagcctt agcgcgtcgt tgttggtcag ctacttgaaa
    32821 agaaggacag cataatgatt accctgaaaa atgtttcaaa atggtatggt cactttcagg
    32881 tgctgaccga ctgctcaacc gaagtgaaaa aaggcgaagt ggtggtggtt tgcggcccgt
    32941 ctggttccgg caaatcaacg ctgattaaaa ccgtcaacgg cctcgaacca gtgcagcaag
    33001 gtgaaatcac cgtcgatggt atcgtggtta acgacaagaa aaccgatctg gcaaagctgc
    33061 gctcccgcgt cgggatggta ttccagcatt tcgagctgtt ccctcatctg tcgattatcg
    33121 aaaacctgac cctggcgcag gtgaaagtgc ttaaacgcga taaagcgccc gctcgtgaaa
    33181 aagcattgaa attgctggag cgcgtgggac tttccgcaca tgccaataaa tttccggcgc
    33241 agctttccgg cggtcagcag cagcgcgtgg caatcgctcg cgcattgtgt atggctccta
    33301 ttgcgatgct gttcgacgaa ccgacatcgg cgctggatcc ggagatgatc aacgaagtgc
    33361 tggacgtgat ggtggaactg gcgaacgaag gaatgaccat gatggtggtg acccacgaaa
    33421 tgggctttgc ccgcaaagtg gcgaatcggg tgatctttat ggacgagggt aaaattgtcg
    33481 aagactcgcc gaaagacgct ttcttcgatg atccgaaatc ggaccgcgca aaagacttcc
    33541 tcgcgaaaat cctgcattaa tcactctggc gcacataccc aattgcgcgc catctcacgc
    33601 tttatcgttt cgcccctcgt atctgttaca tcctggcgtt acccttgtcg caaagaagca
    33661 cacaacaagg agcaacaatg gcactgccaa ttctgttaga ttgcgaccca ggtcatgatg
    33721 acgctatcgc aatagttctc gcactcgcct caccagagct tgatgtcaaa gcaattacgt
    33781 cttccgccgg aaaccagaca ccagaaaaaa ccttacgcaa tgttctgcgt atgctgacct
    33841 tgcttaatcg caccgatatt ccggtggcag gcggcgcggt aaaaccgtta atgcgtgagt
    33901 tgattatcgc ggacaatgtg cacggcgaaa gcggtctcga cggcccggca ttaccggaac
    33961 cgacgttcgc accgcaaaac tgtacggcgg tggagctgat ggcgaaaacg ctgcgcgaaa
    34021 gtgcggaacc tgtcacgatt gtttctaccg gaccgcaaac taacgttgcc ttgctgctca
    34081 atagccatcc ggaactgcat agcaaaattg cccgtatcgt gattatgggc ggcgcaatgg
    34141 ggcttggtaa ctggacgcct gcggctgaat ttaatattta cgttgacccg gaagcggcag
    34201 aaattgtctt ccagtcaggg atcccggtgg tgatggccgg tctggatgtt actcataaag
    34261 cgcaaatcca cgttgaagac accgagcgtt tccgcgcgat tggcaaccct gtttcaacca
    34321 ttgttgccga actgctggat ttcttcctcg aatatcataa agacgaaaaa tggggctttg
    34381 tcggcgcacc gctgcatgac ccatgcacca tcgcctggct gttgaaaccc gagttgttta
    34441 ccactgttga gcgctgggtt ggcgtggaaa cacaggggaa atatacccag ggtatgacgg
    34501 ttgttgatta ttattatctg actggcaata aaccgaatgc cactgtaatt gtcgatgttg
    34561 atcgtcaggg ctttgttgat ttactggctg atcgtctgaa attttacgct taacactcgt
    34621 cattgattaa gatcaccacg cgttacctgg gtggtcttaa tctatttatc tctttttctt
    34681 tcaagatata aatgcaatgg ataatgcaga actcgccata ggtatcgatc tcggcactac
    34741 caatagttta attgccgtct ggaaagacgg tgccgcgcaa ttaattccaa ataagttcgg
    34801 tgaatattta acaccatcca taattagcat ggatgaaaat aatcatattt tagtcggaaa
    34861 accggctgta tcacggcgta cttcgcatcc ggataaaacg gcagcgttat ttaaacgtgc
    34921 aatgggcagt aataccaact ggcggttagg cagcgacaca tttaacgcgc cagaactgtc
    34981 ctctttggta ttacgctcat taaaagaaga tgccgaagaa tttctgcaac gtccgattaa
    35041 agatgtggtg atctccgttc tggcttattt cagcgatgaa caacgcaagc atacccgttt
    35101 agcagcggac ttagccgggt taaatgcggt acgcttaatt aatgaaccca cagcagctgc
    35161 gatggcgtat ggcctgcata cccaacaaaa tacccgttcg ctggtgtttg atctcggtgg
    35221 cggcacgttt gacgttacgg tgcttgagta cgccacgccg gtgattgaag ttcacgcctc
    35281 cgctggcgac aacttcctcg gtggcgaaga ttttacccat atgttggtcg atgacgtttt
    35341 aaagcgcgcg gatgtcacaa ggaccacgct taacgagagt gaactggcag ccttgtacgc
    35401 ctgtgtggaa gcggcaaaat gtagcaatca atcgccaatg cacattcgct ggtagtacca
    35461 ggatgaaacg cgggaatgcg aattttacga gaacgaactg gaagatttgt ggttgccgct
    35521 gctcaatcgc ttgcgagtgc cgattgaaca ggcgttgcgc gatgcgcgtc tgaagccgag
    35581 tcaaatcgac agtctggtgc tggttggcgg cgcgtcacaa atgccgctgg tgcagcgaat
    35641 cgccgtgcgt ctgtttggca aattaccgta tcaaagttac gatccgagca ccattgtcgc
    35701 gctgggcgca gcaatccagg ccgcctgccg cttacgcagt gaagatattg aagaagtgat
    35761 cctcactgat atttgccctt actcgttggg cgttgaggtt aaccgccagg gcgtttccgg
    35821 cattttctcg ccgattattg aacgaaacac cactgtgccc gtgtcgcgtg tagaaactta
    35881 ttcaaccatg cacccggaac aggattcaat tacggttaac gtctatcagg gagaaaacca
    35941 caaagttaaa aacaacattc tggtggaatc cttcgatgtg ccgttgaaga aaaccggggc
    36001 ttatcagtcg attgatattc gctttagtta tgatatcaac gggttgcttg aagttgacgt
    36061 gcttctggaa gacggcagcg ttaagtccag agtgattaac cacagcccgg taacattgag
    36121 cgcgcagcag attgaagaga gtcggacgcg gttatccgca ttgaaaattt atccgcgcga
    36181 tatgctcatc aatcgcacct ttaaagccaa actggaagag ttgtgggcgc gggcgctggg
    36241 tgacgagcga gaagagattg gccgggtgat caccgatttt gatgcggcgc tgcagtcaaa
    36301 cgatatggcc cgcgtcgatg aggtcaggcg gcgggcgagc gtttatttag ccattgagac
    36361 ctcataatca cggctgccgg atgcggcgta aatgctgcat ccggcatcgg cgcaaaaaac
    36421 acatcgtcaa ctaaacaaca tcgcgatttt gccgagagca taacctataa ccagcaccgt
    36481 taaactgatt ttgataatgc tggtaatgct ctgtggtttt tcaccctcat catttttttc
    36541 ttcatggttt tcaggtgttg gctcatcgac atcttcatcc tccggctcga gttttacttt
    36601 cgtcaacagc atccgggtga taatggcctc taatggatga gcatctccgc cttccagcct
    36661 tgtattaaga cgggccagca tctcttgttt cggaaacgct tcaagtacta cctgttccgc
    36721 tacccgatac aaatccagta acggtgaatc ttcttctgtc tcgctaccga ctctcgattt
    36781 ccaccacgcc catgctttgc gtttgaagaa taacgactca ataaacgatt gacctaacgg
    36841 cgataatttt ccgctatgta aaatttccgc ccataacagc cgcgtccggg cgctctcacc
    36901 aggaacatat ttcagcggtt gaccttgcgc atctaccatg ctccgcgctt cgataaccat
    36961 cactaataac gcgaaataat ccgggcaatg acgcttgctc cactgcaaca acaaattatc
    37021 agcttgtgta gatgggtaac tttcccagta cgcgccgaga tcagcaagca ggctttcccc
    37081 ctcgccgcag tacaaacgtt gtgcacactg gtaataccgg gcatcttcgc tttctggttc
    37141 ttccgcttgc cactgtttgg ctacaggaat gagttccacc atacctgttt gcaccgagct
    37201 gtaccagcgc aacagtttgc gatgtaactt tgcatcatca ggaatgaacc acggtccttc
    37261 catcgccaaa aacgcagtga catagtcagg atggtaacgg cagcaacgtt ccagcgcgaa
    37321 gtaatattca acagtctggt tctgtaacgc gacgggatat tccagaaggc taaggaaatc
    37381 gaaacaatca ccagcctgaa tggcctcaag aaaagcctcc ctttcttcct catccattcc
    37441 ttcgctgtca ttaaacgaat gccagttcaa acgctctgcc aacaaagaag cacaatttaa
    37501 tgacagatat cgcgcttcta tagcgattgc acacagcggc cagcgtaatt gatcgacatc
    37561 ctccatgttc caggtattta attgctgaat aaatttctgc caggcggagg gctgaaaacg
    37621 atcactttct gaatccagta atgtcctgaa tgcacgtagt atttcatgtt cagcggtggc
    37681 atctttttct tcatcatctg cttcctcgac aggatttacc gccagtcgca gcgcctcttc
    37741 ataggcctgg cgtaatgctt taaacccctg cggatcggtt tcgggatgac acaacggtaa
    37801 gcgagcaaga taagcctggc ggataatgtc tatttgcgtc gtgctttcaa tttcgaggat
    37861 ttgccaacat gttttcatag gtcgctcaca tccttaagcg ttaccgggca ttcaggctgg
    37921 acagttaatg gctgccaggg aagcgtggca tagaaacggt cgcttcccgc tgccagctct
    37981 tcgaacatcc gactcccggc gctatcgtat ttgtacaggg ttagcgcctc gcgtagcgaa
    38041 gcgttgtctt cgcactgaga agattgccag tagagacgcc ctagtgaata ggcagaaaaa
    38101 tagtgcatcc agctgtgata gtaatggtga gctcgtgcat agacgcgcga ttgcagccat
    38161 aaactctctt cttccgtcag ccagttgttg agcacaccgg cccgtgacag aaatcccatt
    38221 cgaacataat cccaggcttt gattccccct tctccacagc acatagcggt ttgggcaacg
    38281 aattgggtat aaatcaaccc tctctccgag ccaccagcaa ttaatgcttt ccactcttca
    38341 ggggaagagc gttgccatgt cagatataag ccagctaaat cattggcatg accatcatca
    38401 gtcatctgat aaatagtctg aattaaatca tcacgattat ttattcccca ggatgatgag
    38461 agatcgaatt cttttatccc ggtatacatt tttggcgagt catgcgcggg tttgcaactc
    38521 tgattaagta tttccatggg cgcagaaagc gcaaaaagaa gatattgttc ttccttattc
    38581 attttttaat aatcccttaa gtgc
//
LOCUS       DAZSIO010000020        37589 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZSIO010000020 DAZSIO010000000
VERSION     DAZSIO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.020"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(24..278)
                     /locus_tag="ACHX5T_000925"
     CDS             complement(24..278)
                     /locus_tag="ACHX5T_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR8466221.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="ACHX5T_000926"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="ACHX5T_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR8466222.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="ACHX5T_000927"
     CDS             1228..2694
                     /locus_tag="ACHX5T_000927"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR8466223.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="ACHX5T_000928"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="ACHX5T_000928"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR8466224.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="ACHX5T_000929"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="ACHX5T_000929"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR8466225.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="ACHX5T_000930"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="ACHX5T_000930"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR8466226.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="ACHX5T_000931"
     CDS             5709..7265
                     /locus_tag="ACHX5T_000931"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR8466227.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="ACHX5T_000932"
     CDS             7347..9161
                     /locus_tag="ACHX5T_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR8466228.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="ACHX5T_000933"
     CDS             9162..10256
                     /locus_tag="ACHX5T_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR8466229.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="ACHX5T_000934"
     CDS             10256..11281
                     /locus_tag="ACHX5T_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8466230.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="ACHX5T_000935"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="ACHX5T_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR8466231.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="ACHX5T_000936"
     CDS             complement(12876..13220)
                     /locus_tag="ACHX5T_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR8466232.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14749)
                     /gene="bcr"
                     /locus_tag="ACHX5T_000937"
     CDS             complement(13553..14749)
                     /gene="bcr"
                     /locus_tag="ACHX5T_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFR8466233.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVL
                     VWLSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVL
                     SYMLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVR
                     RIGALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVIL
                     DEFPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYA
                     SRPKKR"
     gene            complement(14777..15472)
                     /gene="rsuA"
                     /locus_tag="ACHX5T_000938"
     CDS             complement(14777..15472)
                     /gene="rsuA"
                     /locus_tag="ACHX5T_000938"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR8466234.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15622..17382
                     /locus_tag="ACHX5T_000939"
     CDS             15622..17382
                     /locus_tag="ACHX5T_000939"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR8466235.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17507..17791
                     /gene="rplY"
                     /locus_tag="ACHX5T_000940"
     CDS             17507..17791
                     /gene="rplY"
                     /locus_tag="ACHX5T_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR8466236.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17930..18937)
                     /gene="yejK"
                     /locus_tag="ACHX5T_000941"
     CDS             complement(17930..18937)
                     /gene="yejK"
                     /locus_tag="ACHX5T_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR8466237.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19119..19346
                     /locus_tag="ACHX5T_000942"
     CDS             19119..19346
                     /locus_tag="ACHX5T_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR8466238.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19366..21126
                     /gene="yejM"
                     /locus_tag="ACHX5T_000943"
     CDS             19366..21126
                     /gene="yejM"
                     /locus_tag="ACHX5T_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR8466239.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21201..21277
                     /locus_tag="ACHX5T_000944"
     tRNA            21201..21277
                     /locus_tag="ACHX5T_000944"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21235..21237,aa:Pro,seq:ggg)
     gene            complement(21380..23971)
                     /gene="yejO"
                     /locus_tag="ACHX5T_000945"
     CDS             complement(21380..23971)
                     /gene="yejO"
                     /locus_tag="ACHX5T_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFR8466240.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24291..24938
                     /gene="narP"
                     /locus_tag="ACHX5T_000946"
     CDS             24291..24938
                     /gene="narP"
                     /locus_tag="ACHX5T_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR8466241.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25149..26201)
                     /gene="ccmH"
                     /locus_tag="ACHX5T_000947"
     CDS             complement(25149..26201)
                     /gene="ccmH"
                     /locus_tag="ACHX5T_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR8466242.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26198..26755)
                     /gene="dsbE"
                     /locus_tag="ACHX5T_000948"
     CDS             complement(26198..26755)
                     /gene="dsbE"
                     /locus_tag="ACHX5T_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR8466243.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26752..28695)
                     /gene="ccmF"
                     /locus_tag="ACHX5T_000949"
     CDS             complement(26752..28695)
                     /gene="ccmF"
                     /locus_tag="ACHX5T_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR8466244.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28692..29171)
                     /gene="ccmE"
                     /locus_tag="ACHX5T_000950"
     CDS             complement(28692..29171)
                     /gene="ccmE"
                     /locus_tag="ACHX5T_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR8466245.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29168..29377)
                     /gene="ccmD"
                     /locus_tag="ACHX5T_000951"
     CDS             complement(29168..29377)
                     /gene="ccmD"
                     /locus_tag="ACHX5T_000951"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR8466246.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29374..30111)
                     /gene="ccmC"
                     /locus_tag="ACHX5T_000952"
     CDS             complement(29374..30111)
                     /gene="ccmC"
                     /locus_tag="ACHX5T_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR8466247.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30153..30815)
                     /gene="ccmB"
                     /locus_tag="ACHX5T_000953"
     CDS             complement(30153..30815)
                     /gene="ccmB"
                     /locus_tag="ACHX5T_000953"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR8466248.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30812..31435)
                     /gene="ccmA"
                     /locus_tag="ACHX5T_000954"
     CDS             complement(30812..31435)
                     /gene="ccmA"
                     /locus_tag="ACHX5T_000954"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR8466249.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHR
                     DGDAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31448..32050)
                     /gene="napC"
                     /locus_tag="ACHX5T_000955"
     CDS             complement(31448..32050)
                     /gene="napC"
                     /locus_tag="ACHX5T_000955"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR8466250.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32060..32509)
                     /gene="napB"
                     /locus_tag="ACHX5T_000956"
     CDS             complement(32060..32509)
                     /gene="napB"
                     /locus_tag="ACHX5T_000956"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR8466251.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32506..33369)
                     /gene="napH"
                     /locus_tag="ACHX5T_000957"
     CDS             complement(32506..33369)
                     /gene="napH"
                     /locus_tag="ACHX5T_000957"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR8466252.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33356..34051)
                     /gene="napG"
                     /locus_tag="ACHX5T_000958"
     CDS             complement(33356..34051)
                     /gene="napG"
                     /locus_tag="ACHX5T_000958"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR8466253.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34058..36544)
                     /gene="napA"
                     /locus_tag="ACHX5T_000959"
     CDS             complement(34058..36544)
                     /gene="napA"
                     /locus_tag="ACHX5T_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR8466254.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36541..36804)
                     /gene="napD"
                     /locus_tag="ACHX5T_000960"
     CDS             complement(36541..36804)
                     /gene="napD"
                     /locus_tag="ACHX5T_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR8466255.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36794..37288)
                     /gene="napF"
                     /locus_tag="ACHX5T_000961"
     CDS             complement(36794..37288)
                     /gene="napF"
                     /locus_tag="ACHX5T_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR8466256.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37397..37561
                     /gene="yojO"
                     /locus_tag="ACHX5T_000962"
     CDS             37397..37561
                     /gene="yojO"
                     /locus_tag="ACHX5T_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFR8466257.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
BASE COUNT         9177 a        10161 c         9284 g         8967 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgacctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttatg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccatgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc gacagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgact atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgagg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccgcagcc agcccgtgga agaaacgcat cacaatcagc tgatcgatgg
    14461 tttgtgccaa cgcacacgcc accgcggcgg cggcaaacac cagcgtcccg ccaagaacca
    14521 ccggcttacg cccgaagctg tctgccatcg gcccgtagat taactgcccc aacgcaaagc
    14581 ccagaatata agtactgagg gtcatctgcg tactgcccgc cgatacgcca aactgcgctg
    14641 aaattaccgg tagcgcgggc agatacatat caatcgacag cggcatcaac atggccagca
    14701 ggccaaggat aaaaacaata gcaaacgacg aatgctgtcg ggtggtcaca acgggctcct
    14761 gaaagtcatt gaaaagttag acgacgctgg caatttcttc ttcagttaac ggacgatatt
    14821 caccgggggc taaatcagca tccagcgtaa taccgccaat acgttcacga tgcagctcaa
    14881 ccacgtggtt acccacggcg gcgaacatgc gtttcacctg atgataacgc ccttcgctga
    14941 tggtcagacg aacctgcgtt ggggtaatca cttcaagcac cgccggctta gtgagatctt
    15001 tttcgttatg cagctgcacg cctttagcaa attgctctgc cgtatcgtca gctacaggtg
    15061 attccagtgt caccagatag gtcttctcgc aatgatggcg cggagaagta atgcggtgcg
    15121 accactgacc atcatcagtc atcagcacca gaccggtggt atcgatatcc agacgtcctg
    15181 cggcgtgcag tttccacgct accggttcat ccagaaaata gagcaccgtc gggtgatccg
    15241 ggtcgtccgt ggagcaaaca tagccctgag gcttattgag catgaagtaa cgtggaccgt
    15301 gttgctgcgc cagcgggttg ccatcgtaag cgacatcatg ttcagggagc agtttgaacg
    15361 ctgcattacg aacgatttcg ccatcgacgg tgacacgatt gccgcggatt tcacgcccgg
    15421 caatagcacg gctaacgccg agttgctgtg cgataaattt atcaagtcgc atgtgtgtga
    15481 ttttgcctgt aaaaaacgga ggtcgggtaa tgacccgaaa atctgaactg ttgtctgccc
    15541 agtatagcgg tctaataact tccctcaagg gaaaaagaac cgtggcatac tattagcaga
    15601 ataatctacc tacgcgaaac catgattttt acactccgcc catatcagca agaagccgtg
    15661 gatgccacgc tcaaccattt tcgtcgtcat aaaacccctg ccgttatcgt gctgcccacc
    15721 ggcgcaggta aaagcctggt gatagcggaa ctggcacggc tggcgcgtgg tcgcgtactg
    15781 gtgctggcac acgttaaaga actggtggcg caaaaccatg caaagtatca ggcgctgggg
    15841 ctggaagccg atatttttgc cgccgggcta aagcgcaaag agagccacgg taaagtggta
    15901 tttggcagcg ttcagtcggt cgcccgtaat cttgatgcct ttcagggtga attttcgctg
    15961 ttgattgtcg atgaatgcca ccgtattggt gacgatgaag agagccagta tcagcaaatc
    16021 ctcactcacc tgactaaagt gaatccccac ttacgcctgc tggggctgac tgccacgcct
    16081 tttcgattgg gcaaaggctg gatctaccag ttccattatc acggcatggt acgcggcgat
    16141 gagaaagccc ttttccgtga ctgcatttat gagctgccgc tgcgttatat gattaaacac
    16201 ggctatctga cgccgccaga acgactggat atgccagtag tgcaatacga tttcagccgc
    16261 ttgcaggcac agagtaacgg gctgttcagc gaagctgatc tcaaccgtga gctgaaaaaa
    16321 cagcaacgca ttacgccaca tatcatcagc cagatcatgg agttcgccga aaagcgcaaa
    16381 ggggtaatga ttttcgccgc caccgttgaa cacgcaaaag agattgtggg attactgcct
    16441 gccgaagatg ctgcactgat tactggcgac aaccccggcg ctgagcgcga tgtgttaatt
    16501 gaagatttta aagcccagcg ttttcgctat ctggtcaacg tcgcagtact gaccaccgga
    16561 tttgacgccc cgcacgtcga tcttatcgcc attctgcgcc ctaccgaatc agtgagtctt
    16621 taccaacaaa ttgtcgggcg cggtctgcgt ctcgctccgg gcaagactga ttgtttaatt
    16681 cttgattatg cgggtaatcc tcacgatctc tacgcgccgg aagttggtac accgaaaggc
    16741 aaaagtgaca acgttccggt acaggttttc tgccctgcct gcggttttgc caacaccttt
    16801 tgggggaaaa cgaccgccga cgggacattg attgaacact ttggtcgccg ctgtcaggga
    16861 tggtttgaag atgacgacgg tcatcgcgaa cagtgtgact tccgtttccg ttttaaaaat
    16921 tgcccgcaat gtaacgcaga aaatgatatt gccgctcgcc gctgccgcga atgtgacacg
    16981 gtactggttg atccggatga tatgttaaaa gcggcgctac gactgaaaga tgcgctggta
    17041 ttacgctgta gcggcatgtc tttgcagcat gggcacgacg agaaaggcga atggttgaaa
    17101 atcacctatt acgatgaaga cggcgcggat gtgagtgagc gtttccgtct gcaaacgccc
    17161 gcccagcgta ccgccttcga gcagcttttt atccgcccgc atacgcgcac accgggcatc
    17221 cctctgcgct ggatcaccgc cgccgatatc ctcgcccagc aagccttact gcgacatccg
    17281 gattttgtcg tcgcccgcat gaaaggtcag tactggcaag tgcgtgaaaa agtgttcgat
    17341 tacgaaggtc gttttcgtcg ggcgcacgaa ttacgcggtt aatggtagtt ttgattgatg
    17401 tataaggcga ttgagtatag aatctcgccc gcttttgcat acgcaaagca gatcacttac
    17461 ctgttgctgg gtcgcctgta gcaggattaa tttaagagag aaagaaatgt ttactatcaa
    17521 cgcagaagta cgtaaagagc agggtaaggg tgcgagccgc cgcctgcgtg ccgctaacaa
    17581 gttcccggca atcatctacg gtggcaaaga agcaccgctg gctatcgagc tggatcacga
    17641 caaagtcatg aacatgcaag ctaaagctga attctacagc gaagttctaa ccatcgttgt
    17701 tgacggtaaa gaaatcaaag ttaaagctca ggacgtacag cgtcacccgt acaaaccgaa
    17761 gctgcagcac atcgacttcg ttcgcgctta attgctgaat aagttgtaaa aaaccccgct
    17821 ccggcggggt tttttgtatc tgcagattat gcctgattac ggtattgcta ttttttgcag
    17881 gccagataag gcgttcacgc cgcatctggc attcagaaca caaaaccgat taattgccgc
    17941 cagacgtccg gcgctgcaat tggtcgcgca aattcggcgg cgtgccttta atggtcaagg
    18001 tatcggttgc tggatcccag aagatgcgtt cacctaacag catcgcatca aagttaatag
    18061 tcaagccacc gccgctaccg gcaaacttcg tcaactgacg cagtgtgcta cggtctgccg
    18121 ggaagctctc ttccagttcg tagccttttt cagccgcaaa ctccgtgaag ctcacttcgc
    18181 ttacgcctgc cagctctttc gacagcgatt ccagttcgat ctcttcgcct gcctgcagct
    18241 gctcattaca gtagctataa acctgctggc gcacgttctg tcgctctgct ttatccagtt
    18301 gtgcttcggc agtgaaatca tcaaccgctt gcaacagccc acggttttgc gctttcgcgt
    18361 tcaatccttc gctggccccg aggaaatcca taaagaaatc cgccactttg cgccctactc
    18421 gccctttcag gaaagtgaga tagcgggtgg actctggatt ggtttcccat tcggttaaat
    18481 cgatacgcgc aacaatatcc gcatgattga tatcaagata atgggttggg ttgatgtcga
    18541 gattttcgtt aacgcgcata ctgctcaggt tgctcagaac tgccaccagc aaatactcca
    18601 ccgccagata acggtagtgg caaaacagca cgaatccacc gtcagcaaaa ggatatttcg
    18661 ctaattcgtc acgcaggcga ccggttgccg cgcggctaaa tgccagaaag tcctcttcgc
    18721 cctgacgctg taagcgcagc gtctgcgcca gttcactctc ttcgctaaac aagccgtaag
    18781 ctttattttt ggcgctatag acccgatgca gttctgccac catctcaacg acggtttctg
    18841 tcggttccag caatgaatcg cgcaacacca gctcaaggtt ctgctcatca cgcttgataa
    18901 gctggtgcag agcaatctgg ttgatatcca gactcatgat aaactctcct ttaagaccgg
    18961 gcggtattca accaccgcca gttaagcgac gcaataaaag atggcggtca ttttacagaa
    19021 ggcagcgtaa cgagaatgat ttaacgcata aaaaagcaga aaaaaaaccg ttgctacggt
    19081 aatatgttgc cctttcatca actaactgat ttcgatttat gccacaaatt tcccgctaca
    19141 gtaatgaaca ggttgaacaa ttgctcgcag agctgctcaa cgtactggaa aaacataagg
    19201 ctccgaccga cctttccctg atggtgttag gcaacatggt cactaacctt atcaacacca
    19261 gcattgcccc ggcccaacgc caggcaattg ccaactcttt tgccagcgcc ttacagtcct
    19321 ctatcaacga agacaaagcg cactaaggga aacagataac aggttatggt aactcatcgt
    19381 cagcgctacc gtgaaaaagt ctcccagatg gtcagttggg ggcactggtt tgcactgttc
    19441 aatattctgc tttcgctcgt cattggcagc cgttacctgt ttatcgccga ctggccgaca
    19501 acgcttgctg gtcgcattta ttcctacgta agcattatcg gccatttcag cttcctagtg
    19561 ttcgccacct acttgctgat cctcttcccg ctgaccttta tcgtcggctc ccagaggctg
    19621 atgaggtttt tgtccgtcat tctggcgacg gcgggaatga cgctattact gatcgatagc
    19681 gaagtcttta ctcgtttcca tctccatctt aatcccatcg tctggcagtt ggttatcaac
    19741 ccagacgaaa atgagatggc gcgcgactgg cagctgatgt tcatcagcgt gccggttatt
    19801 ttattgcttg aactggtgtt tgcgacgtgg agctggcaaa agctgcgcag cctgacgcgt
    19861 cgtcgacgct tcgcgcgccc gctggccgca ttcttattta tcgcctttat cgcctcgcat
    19921 gtggtgtata tctgggccga tgccaacttc tatcgcccga tcaccatgca gcgcgctaac
    19981 ctgccgcttt cgtacccgat gacggcgcga cgtttccttg agaagcatgg cctgcttgat
    20041 gcgcaggagt atcaacgccg tcttattgag caaggtaatc cagacgccgt ttccgtacag
    20101 tatccgttaa gcgaactgcg ctatcgcgat atgggcaccg ggcagaatgt gctgttgatt
    20161 actgtcgatg gcctgaacta ctcacgcttc gagaagcaga tgcctgcgct ggcaggtttt
    20221 gctgagcaaa atatttcgtt cacgcgccat atgagctccg gcaacactac agacaacggc
    20281 atctttggcc tgttctatgg catctcgccg agctatatgg acggcattct gtcgacccgt
    20341 acgcctgcgg cattaattac tgcgcttaat cagcaaggct atcagctggg gttattctca
    20401 tcagatggct ttaccagccc gctgtatcgc caggcattgt tgtcagattt ctcgatgccg
    20461 agcgtacgca cccaatctga cgatcagacc gccacgcagt ggatcaactg gctgggccgc
    20521 tacgcacaag aagataaccg ctggttctcg tgggtttctt tcaatggcac taacattgac
    20581 gacagcaatc agcaggcatt tgcacggaaa tatagccggg cggcaggcaa tgtcgatgac
    20641 cagatcaaac gcgtgctcaa tgcactgcgt gattctggca aactggacaa tacggtggtg
    20701 attatcactg ccggtcgggg tattccactg agcgaagagg aagaaacctt tgactggtcc
    20761 cacggtcatc tgcaggtgcc attagtgatt cactggccag gcacgccggc gcagcgtatt
    20821 aatgctctga ctgatcatac cgatctgatg acgacgctga tgcaacgcct gctgcatgtc
    20881 agcacacctg ccagcgaata ttcgcaaggt caggatttgt tcaaccctca acgccgtcat
    20941 tactgggtta ctgctgcgga taacgatacg ctggcaatta ccaccccgaa aaagacgctg
    21001 gtgctgaaca ataacggtaa ataccgcact tacaacttac gtggtgaaag agtgaaagat
    21061 gaaaaaccac agttaagttt gttattgcaa gtgctgacag acgagaagcg ttttatcgct
    21121 aactgattaa ttataaatca gttagcgaaa tatcttactt gcaatcggtg tggaaaacgg
    21181 tagtattagc agccacgagt cggcacgtag cgcagcctgg tagcgcaccg tcatggggtg
    21241 tcgggggtcg gaggttcaaa tcctctcgtg ccgaccaaaa atcccaagaa aaaaccaacc
    21301 cttacggttg gtttttttat atctgcaatt aattcgataa acagaccgtg acacatcaca
    21361 gcctgtttat tttctgttat cagaacgtcc agaccacacc cgcctgagta ttccacggcg
    21421 attctacccc tgcgccattg ccatagctga ctgacaaatg accgcttaac gtcggggtaa
    21481 acgatgacct tatcccagcc tgataaacgc cactggttcc agccacatca ttattaaatt
    21541 tgccatcgtc attaactttc acctggttag aatcggcgta ttcctgacgc acggcggctt
    21601 tcagccaggg ttccagcgtc gtaccgtttt gcaggtccat gtgatagctt accgccgttc
    21661 ccgcttcagc gcgtaatatc cgggtatttc ccacatccgc gcgcatgccg tttgataacg
    21721 tgtagtcctg accatctgtg gtaaagccgg taaaggccag atagggtcta acactccaca
    21781 atccgtcaac ccaacggaac ccgctctcaa catgagcacc cgcgccgtta ctattgtaat
    21841 cgccaaacgc tgttgcccca ttactcatct tgccatggat ggtgttggca aaacggtcaa
    21901 ctttcaccac cccatcaaca taggcaccgt tctgatgctc ccaaccggca taagccccca
    21961 gggtatagct atcgatatta ccttttccgc cgcgatcaaa accaatatca gaatgagagt
    22021 aaccaaagaa caagccgcga attgtactgc tttcttcacg ggagaaacgg ctatcgatac
    22081 cgagcgtcag gcccgtcaat gtttgctcaa aaccagctcc cgcatcagtg gtcacgttgt
    22141 tgcgggtgtt aattgccgaa ctccacatca ccgtatcgta actaacgcct tttacgctac
    22201 caagacgctc acgcacggtg tccagttctg catcaaatac cagcggttgt gcggctgcca
    22261 tgttcagcac atcagtggtt gaaggggtaa tttgcgcgcg attctctgcg aggctccagc
    22321 tatggttgcc attatccagc aaggtatatt catacgtacc gatatcaaca acgcctccgg
    22381 cattgcccaa cgtaaatgca gcatcaccgc cgcccgttgt taccagtgta aggctatctc
    22441 ctgctgccgg gctggcaccg gtgtccgtca cgaatatttt gaaatcacct gttgcctgac
    22501 cggtgacgtt gagctgatcg ctctgatgat tggccatatc ggtacgcata taaaaattgc
    22561 cgtttcccga cagggtattg gtggtcaacg taatgaaatt ttccgcactt gccgtcacag
    22621 atgaacgagt cactggctca gccatcataa tcacatttgc gccggacatc gcaaggttgg
    22681 ctaagttgag gcgagtgttt tggttcgtcg tagtgatatc ggcaatgatg gtccatgcgc
    22741 tattttcgag cgaaacgtcc gctttgctgg tatccacggc accatgcgtt ctaaaagaag
    22801 cgccctcaga aaccactacc ttacccacca acatagcggg tgcataattt atttgcggct
    22861 tcatgacctc aagaatgcca tcattccctc tgactgacac gttaaccatt gtgccagccc
    22921 agacattagc gcggccattg ttgattacca tattttcaga gatggctttt gaggaatacg
    22981 tgatacttcc attctggtct gatcgaccga gatcatatac cgcatttgcc tgcactttcg
    23041 taccagtatc ctttcccagc gattgcatag tggcatgctc atcaaggata gtgtcaatgg
    23101 cctgcgtgtc ctccataaca atgagcccgg aaccatcatc cagttcataa ttttttgaca
    23161 caccatcctt tatactaaat tggcctttac tgtttgtacc actcacttcc agcgcattcg
    23221 ttgagacaat taatttcccg cctgcttttt tatcaacgcc agttgcagtc ccgccatcca
    23281 taacctccag taagccgcca ctatccacag tagtattata agcgaagtca ttttcttcaa
    23341 ctcgcaaact tccgccgttt tccaacaaca tattattagc aataccattc ttgatatcga
    23401 actgaccgag acggtttgtt ccgaatacct ccatggcccg cgtggttgtt ttaatagcac
    23461 cgcctgtttt ctgatctacc gcaagcgcaa atcccccctc tttaacctcc agtagtcctc
    23521 ccttttcgat agcggcaaaa acggcccccc catctttttc aacgattaag cgcccgcctt
    23581 taatcgtggt atcattcgct gggccataaa cctgtagcac accattattg agggtagtct
    23641 ggtttgccgc gccacccatt tgaacaattt gataaccatc atttaatact gagtattctg
    23701 cggtaccatt catttcaatg tattgatacc ctccgttaat ttcagtatta ctacttacac
    23761 caaattcttt tatatgctgt tctccgccag gattgatttt cgtattattg gcggtcccat
    23821 aaaccagctg gatatctttt tccaggataa caccatcaac tgtctcacca tactcaactg
    23881 ttgatgccaa tgctatgggt gaatataacg ctgtagccac cagaacagat attgcggaac
    23941 gacaaagaga aacagaacca gattgatgca ttgagctttc atcctatgaa attaattgca
    24001 gttaaaagca ttgggtacag aaaataccca tagctccata cccggagtca gtttttaaaa
    24061 actgtttaaa gaaatgcaca agtattgtga ttgatttttt agttgttttt cttgatgaga
    24121 agctgatgca aaattccgtc tttataataa aaatgatgcc aaagcgaacg acaaggttgt
    24181 agttttcact acatgtccat acataaaatg gggtaacatt cacgcgcctg gtagcgttac
    24241 caacgctacg ctcaaacata atgattctaa taaaacctca ggagactact atgcctgaag
    24301 caacgccttt tcaggtgatg attgtggatg atcatccact tatgcgacgc ggtgttcgtc
    24361 agttactgga gcttgatcct ggctttgaag tggtcgccga agcgggcgaa ggcgcgagcg
    24421 cgatcgatct ggcgaataga ctggatatcg acgtgatctt gctggatctc aatatgaaag
    24481 gtatgagtgg cctggatacc ctcaatgcct tgcgcaggga tggcgttacc gcgcaaatta
    24541 ttatcctgac cgtatccgat gcctccagcg atgtctttgc gctgatagac gcaggcgcag
    24601 acggttatct gttgaaagac agcgacccgg aagtattgct ggaagcgatt cgtaccggag
    24661 cgaaaggcag caaagtcttt agcgaacgcg tcaatcagta cttacgtgaa cgtgaaatgt
    24721 ttggcgcgga agaagatccc ttcagcgtgc tgacggagcg cgagctggat gttctgcacg
    24781 agctggcaca ggggctgtca aataaacaga ttgcatcggt gttgaatatt tccgagcaga
    24841 cagtaaaagt acatattcgc aatctgctgc gtaaactcaa tgtccgctca cgcgtggcgg
    24901 ccaccattct gttcctgcaa caacgcgggg cacaataaaa atagcccgat ggatttatca
    24961 tcgggctgag atttatgaca aacgcaaaac tgcctgatgc gctacgctta tcaggcctac
    25021 gtggatcgat caatttattg aatttacaca attttgtagg ccggataagg cgttcacgcc
    25081 gcatccggca taaactaagc gcactttgtc atcagtctag cccgatggca tcaccatcgg
    25141 gcctcttttt atttactctc ctgcggcgac aaatgttgca tcgcctgcgc gatactacgt
    25201 tcaatcaccg cacggcgagt atcgttggca ggtaagagtt tcaacatcat ctcccacgcg
    25261 gcaacggctt cgccaaatcg ctgctgctca aaggcattaa acgcatacat gcttagcaca
    25321 cggatattgc tatggtccgt tctcaccagc tgacgtagca gttcaccgcc gaggcggttg
    25381 tcgttgggat cagatgaacg agtcaacgct tcagcgtatc ccagtgcggc atcgctgttc
    25441 ttcggatcga ggcgatacgc agtagcgtat gcatcggtgg cgatactggc gttacccagc
    25501 gccatgccaa cgcggcccaa cataatccag ccttctatat ctcccggatt tttttgcagt
    25561 tgagtacgca tccccagcgc aaggcgcgac atctcttctt cgttgagcgg atcggctttc
    25621 gggtccagcg ccctgtccag caacgccgga gcctgtgccg tggcctgctg ccagattttc
    25681 acctgctgat aattgccagt ctggtagtag ctgacgccag ccacaattaa cgccaccaca
    25741 atacccggca gataaacaac atatccggca cgcttacctt ccggcacgct ttgttcagga
    25801 aacgcttccg gcaccacgcg tacccgacgc cgcgagcggg cgtatatgac ccaaccgcca
    25861 atgccaatag ccactactgg cagcacccac agcagcacgg tcagcggcgt taacggcgga
    25921 tcgtaagtga cgaagttgcc gtaacgcgcc accatataat cgacaatctc ttttttactt
    25981 ttaccttcct gcatcagttc atacactttc tgacgcaggt cggtggcaat catcgagttg
    26041 gaatcggcaa tgctgttgtt ctggcatttc gggcagcgca gttcttcagt gagctgacgg
    26101 aactgttgtt cctgtgcttc atctttaaac tgcaacacgt cgatggtcgc cagcgctgag
    26161 ccggagatca tcagcatcag cacgcccaat aaaaacctca ttgtgcggcc tccttactgt
    26221 atttctccca cagcggcttg atctcttctt cccagacgcg aggattgaga tcgcccgcat
    26281 ggcgatagcg aatgataccg ttgccgtcaa taaggaaggt ttctggcgcg ccatagacac
    26341 cgagatccag ccctaacatg ccgtcgccat caaacaggct tagcgcgtaa ggattgccca
    26401 gctctttcag ccagctgatt gccttctggc gatcgtcttt atagttcatg ccgaccacgc
    26461 gaatgccctg cgctgaaagc tgattcagat attgatgttc cgcacggcag gtcggacacc
    26521 aagtcgccca gacgttaagc agtactggtt tgccctgagt cagcacatcc gcctgataaa
    26581 actgccccgg attgtccagt gattcgagac gaaacttcgg cacaggcttg ccaatgagcg
    26641 ccgattccag attggtcgga tcatcccctt cggcattacg cgccagctgc cacagcagcg
    26701 ccgcggcaat cgccaggaag ataatcaacg gaattaacaa tactttgcgc ttcatacggc
    26761 ctccggcgca gttttttgcg gactcacgcg cttacgatag cgaggatcaa acagacacag
    26821 cagtccgccc aacgccatca tcagcccgcc cgcccaaatc cagcgaacaa atggtttgta
    26881 gtaaagacgc acggcccacg cgccgttttc cagctcttca ccgagcgcgg cgtacaggtc
    26941 acgcgtgatg ccgccgtcaa ttgccgcttc ggtcatcatc gacccggcag tgttgtaata
    27001 acgtttttcc gcatacagca ccgtttccgg tttaccctct cgcgttacgc caatagtcgc
    27061 cacaccgccg cgccagttcg ggccagtcac ctctttgaca tcacggaagg tgaagcgata
    27121 ttcatgaata tcgacgctat cgccggactt catgcgcaca tcacgctcaa cgctataatt
    27181 ctggctaaag gcaatgccaa caattgtcac tgccagcccc aggtgagccg ccaccatccc
    27241 ccaataactg aaggtggttt tcgtgccgcg tgaaatacgc agcgcagctt ccgcagttgc
    27301 cagcaccgca atccagcagg ccattgccag gccgagcacc gtcatcgcca caactttgct
    27361 ttcgaacagc cacggcaaca gcagagacag caccagcgta gagatgaagg cgataatcaa
    27421 taaattgcgg attttacgtg ggcgatcgcg cccccagcgc accagaggac cgacaccaag
    27481 cagtagcgca aacggcacca tcagccaggt aaacatggtg ttgaagaacg gttcgccaat
    27541 cgaaatactg cccagtccaa gttgcttgtg caccagcggc agcagcgtcc ccagcaacac
    27601 caccagcatc gcggcgacca gcaaaacgtt gttcgctaac agcaaagatt cccgcgacca
    27661 cagcgcattg tttacgcgtg agcgaacttt gtgtccacgc gcggcaaaca gcagcagcga
    27721 accgccaatc accagcacca taaaggcgag gataaacata ccgcgcgccg gatcagacgc
    27781 aaacgcgtgt accgatacca gcacgccgga acgcacgagg aaagtcccca acagacacaa
    27841 cgagaaggca ctgattgcca gcagtaatgt ccacgctttg aagctggcgc gttgttcagt
    27901 gaccgccagt gagtgcatca gtgcagtccc caccagccac ggcataaacg aggcgttttc
    27961 taccggatcc cagaaccacc agccacccca gccgagttcg taataggccc atgcggaacc
    28021 gagcacaatg ccgagcgtta ggaaaatcca cgccgccagc gtccacgggc gagtaaaacg
    28081 cgcataagtg ctgtccagac gcccgctcag caaagaagca atggcaaaag caaacgccac
    28141 cgagaaaccc acgtacccca tataaagcag aggcggatgg aagatcagcc ccggatcctg
    28201 taacagcggg ttaagatcgc gcccttcaat cgggaagttc ggcagcgtgc gtgagaacgg
    28261 gttagaggta aagagaatga acagcaaaaa gccgacgctg accatcccca ttatcgccag
    28321 tacacgggcc acaatatcca gcggaatacg ctgactaaaa atcgccaccg caaaggtcca
    28381 gccgctcatc agcagcaccc acagcagaag cgaaccttca tgcgcgcccc aagtagccgc
    28441 cacgcgatac cacaccggaa gctgggtatt ggagttgctg gcaacatagg tgacggtaaa
    28501 gtcgttgacc acgaaagcat tgaccagcac cagaaatgcg ccagccacag acataaacag
    28561 cagccaggca aacaagcggg aagacgccat catgcgcgca tctccgcgcg ccacgcccca
    28621 tagcggatac acggacagca gcagcgcaat ccctaacgcc aagcacagca gcccgtttcc
    28681 aatttccggc atcatgatgc tgggtcctta taaacactcg ccgggcgacg gtggttagct
    28741 tccatcgctt tctcaacttc tggcggcgtg tagttttcgt cgtgtttcgc cagcacttct
    28801 ttcgcgagga tatgattgcc tttttccagc tcgccctgca ccacaacgcc ctgcccttca
    28861 cggaacagat ccggcaaaat gccttcgtaa gagacatcca ctgagccttc agcatcgtaa
    28921 atggtgaagg tcactttcag cgaattggga tcgcgctgca cgctacccgg catcaccatc
    28981 ccgccaacgc gcagacgctg accgacttcc ggcatctgct gagtttcacg cttgccgtag
    29041 agaatttccc ccggcgtata aaagagatcg atattcgagc gcagcgcata tagcaccaga
    29101 ccgatagtca gcgctagccc tgccaacacg gcacaggcaa tccacaagcg gtttttacgg
    29161 cgaatattca tgcagcctcc tgctgttgcg cagcacgtaa acgcgcctca cgcgcccgct
    29221 gttgcgccac accacgcaga attgcgcgat gttgcatcac cgagtgcacg accaaaacca
    29281 ccagcggaat aacggtcatc accaccgcca gccagacaaa aaaggcgtaa ccgcccattg
    29341 cgaaaaattc attccaggaa gcaaatgcag gggtcattta cggcctcttt tcagtatcag
    29401 ttcactcacc cacggacggc gtttttccat cagcaaaatc aaattacgca tccgcatcag
    29461 cgtcagcgtg gcagacagga gcaggaagcc aaaaatcgac cagcgcagcg gcgaacgcat
    29521 cgccggatcg atactttgct gcatccgcgt tgatccctga tgcagggtgt tccaccactc
    29581 cacggagtaa tgaataatcg gcagattcac cacgccaatc agcaccatga tacctgccgc
    29641 acggcccgcc agacggcggt cgtcgaaggc gtgccacagg gcaatcacac ccacatacaa
    29701 aaacagcagc accagttcag aagtcagacg tgcatcccat acccaccagg tgccccacat
    29761 cggttttccc catgcagagc cggtaaccag ggcaataaag gtaaacacgg caccaatggg
    29821 ggccatcgcc gccaccgcca ggttggccat tttcatctgc cagacaaggc caataaacgc
    29881 cgccaccgcc attgatgcat aaatgcccat cgaccagatc gccgcaggca catgcaggta
    29941 gataatgcgg tagctatttc cctgctgata atcagccgga gcaaagccga atccccagat
    30001 ccagccgacg gtaagcacga ccacactggc aattgccagc cacggtataa accagccaca
    30061 gatttgatac agccgtggtg ggatcgccag ttgatgcagt gttttccaca tagtttcgat
    30121 accagactcg aacaaaaatc agtaatccag cgttattgaa tgctgattcg taacgctgcc
    30181 gccgtcgcaa aaggacttaa tgtcgcggtg cctgccagca acgcgcctaa aattgccaga
    30241 tacccatcaa cgggcaaatg catagaagcc gcgtccatcg cggcggtggc aaagatgagt
    30301 aatgggatag tcagcggtaa caccagtatg ctgagcagca caccaccgcg cttaagtccc
    30361 actgtcagcg ccacgcccgg tgcaccgaga aagccaagcg taggcgttcc cagcagcagc
    30421 gtcagcgcca tcacttgcca gccataaaca tccattccca gtagcattgc taccagtggc
    30481 gaaaggatga gtaacggcag accggttacc atccagtgcg ccatcacctt cgccagcaca
    30541 acggcgggca agggtaacgg caacaacatc aattgttcaa gactgccgtc ctgcaaatcg
    30601 tcacggaaca gtcgttccag cgccagcaag gatgaaagca gcgcagcaac ccagataatg
    30661 cccggtgcaa tacgcgccag cagttgcggc tccggaccga tactgagcgg aaaaagggta
    30721 attacaatca ggaagaacca cagcgggttg gcgatttcgg cgctatggcg aaacgctacg
    30781 cgcagttcaa gacggaaaat acgccagaac atcatgcggc cctcgtttgc gtcagtgaaa
    30841 tgcggcgaat tttactttca gcaacgttga gcggctggtg ggtagtcaga atcacaatcc
    30901 ccccctgctc cgtatgctgc gccatacgct gggtcagacg atcgacgccg ttaacgtcaa
    30961 tcgcggtaaa aggctcgtcg aggatccata atgtggcacg ggtcaaccac agacgcgcta
    31021 aagcgacgcg gcgttgttgc ccggcagaaa gttgatttac cggaatatct tcgaatccgg
    31081 caagcccggc ctgcgccaag gcttccagac attgtgcggc atcgccatcg cgatgataaa
    31141 agtgcagatt ttctaacgcc gtcagccggg ttttgatccc cggctgatgg cctatccata
    31201 acagggtttg atggtagctg tcgcgtacct gatgcaaggg ctgcccttgc cagagaacct
    31261 cgccagcgtc agggcgagac aaccccgtca gcaaacggag aagcgttgtc ttccccgcgc
    31321 cgttgctacc ggtgatttgt acccactctc ctgcgttcag cgtaaatgac aagccactaa
    31381 ataaggttcg ttcatcccgc tcacaaagta actctctggc ttcaagcata cccacgcaat
    31441 aaccctgtta aaaacctggc tcgacttcac gcatatcggg cagcttgtgc gcgatccctt
    31501 tatggcaatc aatacaggtt tgcccatctt tcaccgcctg gtcatgcatc ttcgcggcaa
    31561 ccgatttctg ggcggttgta tccatatact cgaagttgtg acagttacgg cactcctgcg
    31621 agttattgtc cttcatgcgc cgccactcat tctgtgccat cgtcagacga tgagcttcaa
    31681 atttctgcgg cgtgtcaata acgccaaaaa ttttaccata cagctcttta cttgctttga
    31741 gcttgcgtat catcttcggc acaaactcgt gcggaacgtg acaatccgga caggtcgcac
    31801 ggacgccgct acggttgttg tagtgcacgg aatccatgta ttcctgatac accgtgttgc
    31861 gcatttcgtg gcagctaatg cagaactctt cggtattggc tttttccatc ccggtgttaa
    31921 agccccccca gaagacgatg ccgccaacaa aaccgatcaa cagcagcgtc cccagcgcca
    31981 gatggctggg ggtacgccac catttccaca ggcgcttaat cagaccaggc ttacggtcag
    32041 aatttcccat aataacctct tatttcccgt aaccttttga tggggtaaag gtattcccca
    32101 cgattggcgc ggtatcggcc tgcggtacgt gacattgcag acagaaataa cgacgcggag
    32161 ccacttccgc gcccactttg ccgtcgctgt ccataaagtg agtaggactg atacgcggcg
    32221 cgccagtggt gcgatagctt tcgacaccgt ggcactgcaa gcagcgattg gtattggtcg
    32281 ttacctgata accttcaacg ctatgcggga tcattggcgg ctgattcaca tagttcagcg
    32341 gcatccgatc ctgctctttt ggcatccgaa tggccccttc ctgcgtcccg gatacttccg
    32401 gcgactggct aaaatcgacg ccattagccg cccaaaccgc gccgcttacc accagggcca
    32461 gcatcgccgt ccattgacac agcgctttct tcaggtcatg gcttttcatg atttcgctcc
    32521 cgaactccat cgtgtagtta ttgtaaatac atcctcagaa cagacatcca cgcagcgacc
    32581 gcacgtcatg caatcgcggc tggtgacctg caccgggctt tgttcatcca gcaccggggc
    32641 acgtagcaca tgcggttccg ggcaaacatg aaaacaatcc atacagcggt tacatttctg
    32701 ccgatcgctg gccgcaacgg taatcacacc tttgctaccc agcacgccat acagcgcgcc
    32761 aaccggacaa atgtgcccgc accagccgtg ttcaacgacc agtagatcaa ataaaaacag
    32821 cgcgagaata agcagcgcgc cgctgccgaa gcccattacc aggctacgcc ccatcagaga
    32881 aacggggttg atccattccc aaatgagcgt gccagttaag gctgatccca ccagaatgac
    32941 caccaacagc acgtagcgaa tgtggcgagg gatcgtcgca gactgattga ggtcaaagcg
    33001 cctgcgtaac cagttcgcta agtcggtaat cgggttcagc gggcagaccc agctgcaaaa
    33061 taatcgcttt cccgccaggg cgtagagcac ggtgataatc accgcaccgg tcaacgccac
    33121 cgtggcaggc agatgaccac tggcaagact ttgcagcgtc atcagcggat cggtcagcgg
    33181 aacggtgtcg aataacaggc tactgctgta gttgccgtgc aagatccaca caccaaacca
    33241 tggaccgctc aaaaacatcc ccagcacgaa gaactgacaa aggcgacgta acaccagcca
    33301 acggtgactg cgccaccagc cttttttctc cagcgcctcg cgcccggcgt cacgtttacg
    33361 atttgccatt gttcccctcc agccagccga agcggtaatg gtgacctaac tcccctttcg
    33421 ccagtgacag cggtaacacc ttgattgccg gttgttccag cacgcatact ttttcgcact
    33481 taccgcaacc ggtacaggcg tcgctgtgaa ccgtcggcag aaagcgggcg tgcttaccgg
    33541 tacgcgtgtt gcgctccagc tccaaggtga tggcctcatc aattttcggg cattcgcgat
    33601 aacaaacatc gcagcgcagc ccctgaaagt tgagacagtt ttcctggtcc accagtaccg
    33661 ccagccccat ccgcgcgtcg tcgatcgatt caatctcacg atccagcgca ccgcttgggc
    33721 acactttggc gcacggaatg tcctcacaca tttcgcaagg aatatcccgt gcgacaaaat
    33781 atggcgtgcc cgccgacaga ccagaggcca gcgtcgccag ttttaaggtg tcgtaagggc
    33841 aagcctgaac acactgaccg caacgcacac aggcactggc aaaggcgttc tcgtttatgg
    33901 ctccgggcgg gcgcaaccgc acgccagatg cgcgtgcggt ttgctgttgt aaccccagcg
    33961 ccacacccac ggcagccagc ccgcctgctg tgcgaacgac atcgcgcaga aagcggcggc
    34021 gaccattttg aggtttcgct gaccgggaca taatggctta caccttctcc agtttgaccg
    34081 cgcacttctt gaagtccgtc tctttcgaga gcggatcggt cgcatccagc gtcagtttgt
    34141 taaccagctg tgcggcgtcg aagaacggca tgtacaccag gccctgtggc ggacggttac
    34201 gaccgcgcgt ttcaacaatc gagatcactt cgccacggcg agaaaccact ttcactttgt
    34261 cgccacggcg cagatcgcgc gctttcgcat ccagcgggtg aataaacaag accgcttccg
    34321 ggaaggcgcg gtgcagttcc ggcacacggc gagtcatact gccggtgtgc cagtgctcca
    34381 gaacgcgtcc ggtagagagc cacaggtcgt actcttcatc cggtgcttcc gccgccggtt
    34441 cgaacggcag tgcgaagatc accgctttgc catccggttt accgtagaac ttatagcctt
    34501 cgcccgcttt cacgtacggg tcgttacctt cgctgtaacg ccactgtgtt tctttaccat
    34561 tcaccaccgg ccagcgcaga ccgcgcgctt tgtggtagtc atcgaacggt gccagatcgt
    34621 gaccgtgacc gcgaccaaac catgcgtact cttcgaacag ccctttttgc agatagaagc
    34681 ccagctcgcg ggattcatcg ttcagctgat cttccgccag ttcggatacc gggaatttgc
    34741 tcacttcggg ggtggcatac agaacttcgt acagcgtttt gccacgcagt tccggtttct
    34801 tcgccagcag ctcttccggc catacttctt cagttttgaa gcggcgggag aactggacta
    34861 actgccagag atccgatttc gcttcgcctg gcgcctgtac ctgctgacgc cagaactgag
    34921 tacggcgttc ggcgttaccg taagcgccct ctttctctac ccacattgcg gtcggcagga
    34981 tcaagtcggc ggccagcgca ctgactgtcg gatacggatc ggagacgatg atgaagttgc
    35041 gcggatcgcg ccagcccggc atacgctctt cattgatgtt cggcccggcc tgcatgttgt
    35101 tggtacacat ggtccagtaa acattcagct tgccgtcttt cagcgcacgg tcttgtgcca
    35161 ccgcatgaag accgattttc gccggaatgg tgccgctcgg gatattccac ttcttctcac
    35221 agatatcacg atgtttctcg ttagttacca ccatgtccgc aggcagacgg tgagcaaagg
    35281 tgcccacttc acgcgcagta ccacacgcag aaggttgccc ggtcagggag aacggaccgc
    35341 aacccggctg ggaaattttg ccggtcagca ggtgcaggtt gtagaccagg ttgttagccc
    35401 acacaccacg ggtatgctgg ttgaagccca tcgtccagta ggagatgact ttcttgttcg
    35461 gatcggcata cagctgcgcc agttgttcta actggtcttt cggcacgccg gtcatttcag
    35521 cggttttttc cagcgtatac tcagcaacga aggctttgta atcttcaaag ctcatcggtt
    35581 cggaggcgtc agaacccgga ttcttcgctg ctttttccag cggatgggtc ggacgtaaac
    35641 cgtagccgat gtccgtcgcc cctttgcgca ggttaacgtg cttgctgaag aagtcctgat
    35701 ttatcgcatt gttttgaatg atatagttgg cgatgtagtt caggatcacc aggtcagatt
    35761 gcggcgtaaa gatgatgccg ttatccgcca gctcgaagct acgatgctgg taggtagaaa
    35821 gcaccgccac ggtgacgttc tggttagaga gacgactgtt agtgatgcgt gaccagagga
    35881 tcgggtgcat ctccgccatg ttagcgcccc acagcacaaa cgcgtcagcc tgctcgatgt
    35941 cgtcatagca gcccatcggc tcatccatac caaaggtacg cataaagcca actactgccg
    36001 acgccataca gtgacgtgcg ttcgggtcga tgttgttcga acggaagccc gctttgaaca
    36061 gcttggacgc ggcataacct tcccagatag tccactgacc agaaccgaac ataccgatcg
    36121 attccggccc tttttctttc agggcggttt tgaatttctc ttccatcaca tcgaaggcct
    36181 gatcccaggt gattggggta aattcgcctt ctttgtcata tttaccgttt ttcatacgca
    36241 gcagcggctg cgtcaaacgg tctttaccgt acatgatctt gggcaggaaa tagcccttaa
    36301 tgcagttcag gccacggtta accggtgcgt ccgggtcgcc ctgacaggcc accacacgcc
    36361 cctgctgcgt tccgaccaga acgccgcaac cagtaccgca gaaacggcac ggcgctttat
    36421 cccatttaat ggcttcctgc tgaccaacaa cggcgcgggc aacgcccggc acgctgagac
    36481 cggcagccgc cgcagcggcc gcaacggcgt tagctttcat aaagctacga cgactgagtt
    36541 tcatggtgtt tcctcacctt gctcttcctg ctggtgataa accagcgaca ccgccagcac
    36601 gccctctacg ttgcgtactg actcaatggt ttggatcagc gtttcgctgt cttctgcttc
    36661 caccaccaca atcaactgac cgctcggcgc gtcgctgaca gcaacttcac agccgggaaa
    36721 ggcgttcagt tgggtgctga tgtctgaaat tcgttcgctt ttggcctgca cgaccaggct
    36781 gcaaacttgc cagttagtgt gcatggagat actccgcagt tatggctgat accggacagc
    36841 tggctgcaca tgctccacat ccgttgcaaa gttggctatt aagttgcgcc tggtagatcc
    36901 cggagagcgt cgggcgaaag ataattgcca ttggctcaca gctatcctga cagcggcggc
    36961 attcaactga ctgatacgcc aggcacgcgt ccccgatggt gaactgcaaa tcccaggccc
    37021 tggtgtggcg cggagaaaat agcgattcgg ggcaagcctg cgcgcaggcg taacagaagc
    37081 tgcactcgtt atttttgaaa ttaacgctcg gatagccacc cgtgccgcgt tgcagaatgt
    37141 tgttttcaca agcattaata caggcgtcgc agcgggtgca atgggtcaga aaatgagatt
    37201 catcaccgga ccagggcgga cggataccgt tactggcttt gcgccagcaa ccagtgagta
    37261 tgccccgacg ggatgcatca atcttcacat tgaccttcca tcattaacgc gctctgaaaa
    37321 ttgagagcga ccaaatagac cgcataatta ataagccatt tttatagccg ctaagatatt
    37381 aaaggatgtg tcaaagatgc ataccccgat cggggtaaaa cctgtagcag gatcaaaaga
    37441 gtggcgggaa gcgtggcaaa aacgggcttt tgctcacatt tcaaatggtt ataaacatat
    37501 ttatatagcg attaattcac cagagatatt tctgctggtt tgctccctca tcagaattta
    37561 acactaaaag agcaggtaaa attgtctga
//
LOCUS       DAZSIO010000021        37429 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZSIO010000021 DAZSIO010000000
VERSION     DAZSIO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.021"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..354
                     /locus_tag="ACHX5T_000963"
     CDS             <1..354
                     /locus_tag="ACHX5T_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR8466258.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="ACHX5T_000964"
     CDS             complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="ACHX5T_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR8466259.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="ACHX5T_000965"
     CDS             complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="ACHX5T_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR8466260.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3109..3261
                     /gene="mokC"
                     /locus_tag="ACHX5T_000966"
     CDS             3109..3261
                     /gene="mokC"
                     /locus_tag="ACHX5T_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR8466261.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="ACHX5T_000967"
     CDS             complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="ACHX5T_000967"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR8466262.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="ACHX5T_000968"
     CDS             complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="ACHX5T_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR8466263.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6877..7281
                     /locus_tag="ACHX5T_000969"
     CDS             6877..7281
                     /locus_tag="ACHX5T_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR8466264.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7307..8020
                     /gene="msyB"
                     /locus_tag="ACHX5T_000970"
     CDS             7307..8020
                     /gene="msyB"
                     /locus_tag="ACHX5T_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR8466265.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8169..8735
                     /gene="satP"
                     /locus_tag="ACHX5T_000971"
     CDS             8169..8735
                     /gene="satP"
                     /locus_tag="ACHX5T_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR8466266.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8770..9357)
                     /gene="mog"
                     /locus_tag="ACHX5T_000972"
     CDS             complement(8770..9357)
                     /gene="mog"
                     /locus_tag="ACHX5T_000972"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR8466267.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9472..10425)
                     /gene="tal"
                     /locus_tag="ACHX5T_000973"
     CDS             complement(9472..10425)
                     /gene="tal"
                     /locus_tag="ACHX5T_000973"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR8466268.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10704..12134
                     /locus_tag="ACHX5T_000974"
                     /pseudo
     CDS             10704..12134
                     /locus_tag="ACHX5T_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            12204..12980
                     /gene="yaaA"
                     /locus_tag="ACHX5T_000975"
     CDS             12204..12980
                     /gene="yaaA"
                     /locus_tag="ACHX5T_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR8466269.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13133..13429)
                     /locus_tag="ACHX5T_000976"
     CDS             complement(13133..13429)
                     /locus_tag="ACHX5T_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR8466270.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13643..14929)
                     /gene="thrC"
                     /locus_tag="ACHX5T_000977"
     CDS             complement(13643..14929)
                     /gene="thrC"
                     /locus_tag="ACHX5T_000977"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR8466271.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14930..15862)
                     /gene="thrB"
                     /locus_tag="ACHX5T_000978"
     CDS             complement(14930..15862)
                     /gene="thrB"
                     /locus_tag="ACHX5T_000978"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR8466272.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15864..18326)
                     /gene="thrA"
                     /locus_tag="ACHX5T_000979"
     CDS             complement(15864..18326)
                     /gene="thrA"
                     /locus_tag="ACHX5T_000979"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR8466273.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18352..18471)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18407..18472)
                     /gene="thrL"
                     /locus_tag="ACHX5T_000980"
     CDS             complement(18407..18472)
                     /gene="thrL"
                     /locus_tag="ACHX5T_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR8466274.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18686..19372)
                     /locus_tag="ACHX5T_000981"
     CDS             complement(18686..19372)
                     /locus_tag="ACHX5T_000981"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR8466275.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19772..19912)
                     /gene="yjjY"
                     /locus_tag="ACHX5T_000982"
     CDS             complement(19772..19912)
                     /gene="yjjY"
                     /locus_tag="ACHX5T_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR8466276.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20008..20724
                     /gene="arcA"
                     /locus_tag="ACHX5T_000983"
     CDS             20008..20724
                     /gene="arcA"
                     /locus_tag="ACHX5T_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR8466277.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20783..22135)
                     /gene="creD"
                     /locus_tag="ACHX5T_000984"
     CDS             complement(20783..22135)
                     /gene="creD"
                     /locus_tag="ACHX5T_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR8466278.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22193..23617)
                     /gene="creC"
                     /locus_tag="ACHX5T_000985"
     CDS             complement(22193..23617)
                     /gene="creC"
                     /locus_tag="ACHX5T_000985"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR8466279.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23617..24306)
                     /gene="creB"
                     /locus_tag="ACHX5T_000986"
     CDS             complement(23617..24306)
                     /gene="creB"
                     /locus_tag="ACHX5T_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR8466280.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24319..24792)
                     /gene="creA"
                     /locus_tag="ACHX5T_000987"
     CDS             complement(24319..24792)
                     /gene="creA"
                     /locus_tag="ACHX5T_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR8466281.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25003..25872
                     /gene="robA"
                     /locus_tag="ACHX5T_000988"
     CDS             25003..25872
                     /gene="robA"
                     /locus_tag="ACHX5T_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR8466282.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25869..26516)
                     /gene="gpmB"
                     /locus_tag="ACHX5T_000989"
     CDS             complement(25869..26516)
                     /gene="gpmB"
                     /locus_tag="ACHX5T_000989"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR8466283.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26568..27088
                     /gene="yjjX"
                     /locus_tag="ACHX5T_000990"
                     /pseudo
     CDS             26568..27088
                     /gene="yjjX"
                     /locus_tag="ACHX5T_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(27173..27499)
                     /gene="trpR"
                     /locus_tag="ACHX5T_000991"
     CDS             complement(27173..27499)
                     /gene="trpR"
                     /locus_tag="ACHX5T_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR8466284.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27589..29526)
                     /gene="sltY"
                     /locus_tag="ACHX5T_000992"
     CDS             complement(27589..29526)
                     /gene="sltY"
                     /locus_tag="ACHX5T_000992"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR8466285.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29737..31404
                     /gene="ettA"
                     /locus_tag="ACHX5T_000993"
     CDS             29737..31404
                     /gene="ettA"
                     /locus_tag="ACHX5T_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR8466286.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31511..31678)
                     /gene="nadS"
                     /locus_tag="ACHX5T_000994"
     CDS             complement(31511..31678)
                     /gene="nadS"
                     /locus_tag="ACHX5T_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFR8466287.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31712..32944)
                     /gene="nadR"
                     /locus_tag="ACHX5T_000995"
     CDS             complement(31712..32944)
                     /gene="nadR"
                     /locus_tag="ACHX5T_000995"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR8466288.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32965..34347)
                     /gene="radA"
                     /locus_tag="ACHX5T_000996"
     CDS             complement(32965..34347)
                     /gene="radA"
                     /locus_tag="ACHX5T_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR8466289.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34396..35364)
                     /gene="serB"
                     /locus_tag="ACHX5T_000997"
     CDS             complement(34396..35364)
                     /gene="serB"
                     /locus_tag="ACHX5T_000997"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR8466290.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35470..36114
                     /locus_tag="ACHX5T_000998"
     CDS             35470..36114
                     /locus_tag="ACHX5T_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR8466291.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36142..37157
                     /gene="lplA"
                     /locus_tag="ACHX5T_000999"
                     /pseudo
     CDS             36142..37157
                     /gene="lplA"
                     /locus_tag="ACHX5T_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37189..>37429
                     /locus_tag="ACHX5T_001000"
     CDS             37189..>37429
                     /locus_tag="ACHX5T_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8466292.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPS"
BASE COUNT         8991 a        10129 c         9256 g         9053 t
ORIGIN      
        1 tatcagtttc atcatataag atttgactgc atccagggcg agcaaggtga attagttttt
       61 gaacccagtg acgttgaata ttactttgaa ccagtgacga tacaggagtc aggaaccagc
      121 atattaaaaa atgagttaga aaacagtgag gacggtgcgg gtcaaatagg ctttcaactc
      181 tccaatgatg gtacgaatga aatccagtat ggtaaaagca attactactc ttttcagcat
      241 ccccatgaag gaagtaatca gattcctcta tttattcgcc cgcgtacata tggaaacaat
      301 gtctcaagcg ggcaaataat gagccgagtc aaaatcgtgg taatgtacaa ctaatctatc
      361 tcccccacct taactttggg ggagctgccg attaacgcac cgctggacta aaaagcgcag
      421 aataatccgt attgcagatt cgctgtaccg ccgggtgctg aatcatccgc tcagcaaaaa
      481 tagcatggta ctcttccatc acattctcga cgcgaccaat ttctacgaca gttttatcgg
      541 cataaaagtc atatgcataa agcgttgggg caacgaagat tgcattgtgc atcgcaccaa
      601 aagctttcat caaagcggca tcatcaaact cgccgaggat ttctacgttt aatccctggg
      661 agttaaacca gttaagcaat ttgcgcccta acattgaacg tcgcccagga atcaaaagtc
      721 gccgttcttc cagacaagcc gggaacggtt tttctggtgg tggatttgta caccagaaac
      781 tcacgccaca ttcgccaatt ctcacggaga acaggccttc ctgctgcgta gagtctatcg
      841 gacagtcaga aatgatcata tccagtttat gctgacttaa ttgctccagc agcatttcgt
      901 gggtggattc gaagcagcga agatgaatgg gctcgccttc taccactgcg gcgttaagta
      961 cgctactgac caggcgtttg gaaagtgcat cagccacgcc aacgtcaaac aataaattgg
     1021 attctttgcg atagttcaca atatccagca tttcctggct taaggtgaac attttatcgg
     1081 catagcgata gaccagttct cccagctcgc tgggttcgag accacgtccc ttgcgtttaa
     1141 ataatttgcc ttgcaggcgc tcttccagcg ctcgaatctg tccggtaatg gtttgtggag
     1201 ttaaataaag cgcctccgct gcgccaacca cggaaccttc tttatagaca tgccagaagt
     1261 aatacaagtg gttgtaattg atatgagaca tgctcatttc tctccctgat aacaatgaaa
     1321 agggagccgt ttatggctcc ccggtaaacc gtcctgtcaa actgatggac gcaaacgaac
     1381 gcgtaaccag ctgtatccaa ttaccgccga agagatagaa ccgaccagga taccgagttt
     1441 cgcccagtta atcagttctg gatctacgct accaaaggcc aggctggcaa taaagataga
     1501 catagtaaaa ccgataccgc acaggatccc caccaccata atttgctgat aagtcgttcc
     1561 ctcaggcaga tgcgccagtt tcaaacgcag cgccaaccag cagaacagac taatccccag
     1621 cggtttgcca atcagcaagc cagcgatgat ccccaatggc agaatggagg tcaagccatc
     1681 cagcgtgacg ccttgcagtg aaacgccagc attagcaaat gcaaacagcg gcaaaatcag
     1741 atacgccacc cacgggtgca acacatgctc cagtcgcttc gctggagaac gcccatgctt
     1801 ctctttcaaa ggaataaaga agccgacaat tacccccgcc agagttgcgt gaacccccga
     1861 tttcaacacc gcagtccaca acaccacgcc aacaagaata tagacgcccg tgcggcgtac
     1921 accacacaga ttcaataccg cgagtaccgc aattgctaca gccgcgacgc caagggaggc
     1981 catcgataag tcattagtgt agaacaatgc gatgataatg atggccccaa gatcgtcgat
     2041 aatagccaga gccatcaaaa agatcttcag cactaacgga acacgacttc ccaacagcgc
     2101 cagtacacca agtgcaaaag caatgtcagt agccgccggg atcgcccacc cttcgcgggt
     2161 aatcggatcg gcatagttaa aagccagata gagtaatgcc ggcacaatca tcccaccaat
     2221 agcggcgata actggaaatg cggcctggcg taagctggct agcgatcctt gcatcagttc
     2281 acgtttaact tccagaccga ctaacaggaa aaataccgcc atcagcgcat catttatcca
     2341 taacagcatg tttttgttga tttcgagtga accaacccgg agctgaaccg gcgtctccag
     2401 aaagtcgtga taccatccac tggttgcgcc gctgttggcc ataatcatcg ccaggatagc
     2461 ggcaatgata agaataatgc ctcccgaggc atcactgcta aagaatcgat gcagatgttt
     2521 cactttttat ttctctttca ggtgaataga tcgtttttac tattttaccc cgcacgaatc
     2581 atcgttaaaa tcagattata gtgtaggaat aattcgtttt ttacgagcca ttgaagaatt
     2641 tgcgccatca gcggtgagat gcgtttttga cggctgtaag cgctcacgag tgaccctaag
     2701 cccagataat catagcttaa ggtgaggatt cctgagggat taattaggaa aagtaataat
     2761 taatttgttg tcagtcggcg atgcttgtta ggccaatatt tcaaaaataa tccagcttgt
     2821 cgtcaataat tgcgatgaag agataaatta aagttaaata attaaatcat taaaaaatta
     2881 cgccacaaaa atgcgaggcg tcttcaggat tagaatcgat aaaacaaaat gggttaaaga
     2941 gagcgatata atagcggcgg gtgcttgagg ctgtctgtct caggcattag ctgaacggca
     3001 gatagagaaa agccccgagt gatattttac catcaacccg aggcctccta tatgctgaac
     3061 acatgtagag tgcctcttac tgaccgtaag gtcaaggaga agagagcaat gaagcagcat
     3121 aaggcgatga ttgtcgccct gatcgtcatc tgtatcaccg ccgtagtggc ggcgctggta
     3181 acgagaaaag acctctgtga ggttcacatc cgaactggcc agacggaggt tgctgttttc
     3241 acggcttacg aatccgagta agagcaacgg cggggagtga tccccgccac tttacaggtg
     3301 ctcgcatatc ttcaacgcac cctattttta cccaggcctg cccacgggca ggcttttggg
     3361 gaggttagcg ggtcaggtcg tcaaaaaact tcttcacacc atcaaagaag ctctttgagc
     3421 gcgggctgtt gtgctcgccg gttgggccac cgaagctttc ttgcagctct tgcagcagct
     3481 gtttctgctt ttcgttcagg cctaccggtg tttcgacgac aacgcggcac agcaaatcac
     3541 cctgtgcgcc accgcggaca gacttgacgc ctttaccgcg catacggaat agcttaccgg
     3601 tctgggtttc gccaggcact ttcagtttga cgcgaccatc aagggtcggt acttcgattt
     3661 cgccacccag cgccgccata gcgaagttga tcgggacttc gcaatacagg ttgttgcctt
     3721 cacgctcgaa aatcgggtgc tgtttaacct gaacctgaac gtacagatcg cctgccggtg
     3781 cgccatgctc gcccgcttca ccttcgcccg caagacggat gcggtctcca gtgtccaccc
     3841 ctgccgggat tttaacggac agcgttttgc tgcgctcaac acgaccatga ccatgacatt
     3901 tgttgcacgg atctttgatc agcgtaccgc ggccctgaca gtgtggacag gtctgctgta
     3961 cagcgaagaa tccctggcgc atctgcacct gaccagaacc atgacaggtc ggacaggtct
     4021 gcggctgtgt acctggtttt gcaccgctac cgtggcaaac gtcacactct tccagagtcg
     4081 gaatgcggat ctctttggtc acgccacgta cagcttcttc gagggtgagc tccatgttat
     4141 agcgtaaatc agcaccgcgc gccgcacgtt gacgaccacg tccgccgcca aaaatatcgc
     4201 cgaaaacgtc accaaaaata tcgctgaagt ctgcgccgcc gccaaaacca ccgccgccca
     4261 tgcctccttg ctcaaacgca gcatgaccat actgatcgta tgccgcacgt ttttgcgagt
     4321 cggtcagaac ttcataagct tccttgatct ctttaaattt cgcctcggcc tctttgtcac
     4381 cctggttacg gtccgggtgg tatttcatgg ccaggcgttt gtaggccttt ctgatttcac
     4441 gctcttccgc tgttttggaa acgcctaaaa tctcgtaata atcttgctta gccatctttt
     4501 ttaaattgcc cctagatgaa tgcacgggcg gagaggaaat tccccttcgc ccgtgtcagt
     4561 ataattaccc gtttataggg cgattatttt ttgtctttga cttcttcaaa ttcagcgtcg
     4621 acaacatcgt catctttcgc gttgtttgca gaagcatcag caccggcagt ctgctgctgg
     4681 gcatgttgct gctgggcgat ttccatcagt ttctgggaaa cctgtgccag ttcctgcatt
     4741 ttcgcttcga tagcggcttt gtcttcacct ttcagagcag tttccagtgc agtcagcgca
     4801 gactcgatag cagttttgtc gtcagccggc agtttgtcgc ctgcttcttc aacctgctta
     4861 cgggtgctgt gcagcagatg gtcgccctgg ttgcgagtct gtaccagctc ttcaaactta
     4921 cggtcagctt cggcgttagc ttctgcgtcg cgtaccattt tctggatttc atcttcgttc
     4981 agaccagaag aagccttgat ggtgatcttc tgctctttac cgctgttttt atctttcgcg
     5041 gaaacgtgca ggataccgtc agcatcgata tcgaaggtaa cttcgatctg cggcatgccg
     5101 cgcggtgccg ggttgatacc atctaggttg aactgaccca gagatttgtt atcagccgca
     5161 cgtttacgtt caccctgcag cacatggatg gttaccgcag actggttgtc ttcagcggta
     5221 gagaacacct ggctgtgctt ggtcgggata gtggtgtttt tcgcgatcag cgtcgtcatc
     5281 acaccgccca tggtttcgat acccagagac agcggggtaa cgtccagcag cagtacgtct
     5341 tttacgtcac cagtcagaac accaccctga acagcagcac cgattgctac agcttcgtcc
     5401 gggttaacgt ctttacgcgg ctctttacca aagaactcag caactttctt ctgaaccatt
     5461 ggcatacgag tctgaccacc aacgaggata acgtcgtcga tatcagatac ggacaggcca
     5521 gcatcctgca gtgcaacttt cagcggctca atggaacggt ttaccagatc ttcaaccagg
     5581 ctttccagtt tcgcacgagt cactttgatg ttcatgtgtt tcggaccggt cgcgtctgca
     5641 gtgatgtatg gcaggttaac gtcggtctgc tgagcggaag acagttcgat tttcgctttt
     5701 tctgccgctt ctttcaggcg ctgcattgcc agcggatcgt tgcgcaggtc aatgccctga
     5761 tctttcttga attcttcaac cagatagttg atcagacggc tgtcgaagtc ttcaccaccc
     5821 aggtgggtat caccgttggt tgccagaact tcgaaggttt tttcgccgtc aacttcgtcg
     5881 atttcgataa tagaaatatc gaaagtacca ccccccaggt cataaaccgc gatagtacgg
     5941 ttgccagtgc ctttgtccag accgtaagcc agcgcagctg cggtcggttc gttgatgata
     6001 cgttttactt ccagaccagc gatacggcct gcgtctttgg ttgcctgacg ctgagcatcg
     6061 ttaaagtatg ccggtacggt gataacagct tcagttaccg gttcacccag gtaatcttca
     6121 gcggttttct tcattttttt cagcacttca gcagaaatct gcggcggtgc cattttctgg
     6181 cctttaactt cgacccatgc gtcgccgtta tcagcagcaa taattttgaa cggcatgatg
     6241 gaaacatcac gctgtacttc ttcgtcctgg aagcggcggc caatcaggcg tttaatcgca
     6301 aacagggtgt tttgcgggtt cgtcactgcc tgacgtttag ccggctgacc aaccagagtt
     6361 tcaccatcct gggtataggc aatgatagaa ggcgtggtgc gatcgccttc ggcgttctcc
     6421 agcacgcgag gaatggtgcc atccataatc gctacacaag agttggtagt acccaggtcg
     6481 ataccaatta ttttacccat ctaaacgtct ccactatata ttcggtcatc atgtggttgt
     6541 gagtctgtaa taggggcgaa acgtctggtt tcaactgccc aatttcattt ttttgcagac
     6601 tcactcactg cggttgacta ctaaatgggg tcgtaaccca cgtcatcaag ggggagatgc
     6661 aaaaattttt tgtgcttttg cgcagaaatt tcgcgatatg gcaggacaag acgtaaaatt
     6721 gacagcaatt ttattcatat aaagaatgaa tcgcagccaa ggcgttcatc aaaaaattgt
     6781 aataaaaaga aaagattacg tgcctgaatc ttctctttat cagcagtaaa ctagtgggta
     6841 ttcatccccc tacctcttcc cactaagaga atccttatga aatccgtttt tacgatttcc
     6901 gccagcctgg cgattagcct gatgctgtgc tgcacggcgc aggcaaacga ccataaactc
     6961 ctcggcgtca ttgcaatgcc gcgtaacgaa accaacgatc tggcgctgaa acttcctgtt
     7021 tgtcgcattg tgaaacgcat acaactctct gccgaccatg gcgatttaca gttaagtggt
     7081 gcatcggttt atttcaaagc cgcccgtagc gccagtcaga gcctgaatat tccttcagaa
     7141 ataaaagaag ggcaaaccac tgactggatc aacattaaca gcgataacga caataaacgc
     7201 tgcgtctcaa aaatcacctt ttccggtcat acggtgaact catcggatat ggccacgctg
     7261 aaaattatcg gcgacgacta acgccagaat atagaagcca caaaaaatga atgttaatta
     7321 cctgaatgat tcagatctgg attttcttca gcattgtagt gaggaacagt tggcaaattt
     7381 cgcccgattg ctcacccata atgaaaaagg caaaactcgc ctctccagca tactgatgcg
     7441 caacgaactg tttaaatcga tggaagggca tcccgagcaa catcgccgca actggcagct
     7501 gattgccgga gaattacagc attttggtgg cgatagtatc gccaacaaac tgcgcggaca
     7561 cggtaaattg tatcgggcca ttttgctcga tgtttcaaag cgattgaagc tgaaagccga
     7621 caaagagatg tctacgtttg aaattgagca acagttactg gaacaatttc tgcgtaatac
     7681 ctggaagaaa atggacgagg aacataagca ggagtttctg cacgcggtcg atgccagggt
     7741 gaatgagctg gaagagctgc tgccgctgct gatgaaagac aaattattgg caaaaggtgt
     7801 gtcgcatttg ctttccagcc aactgacccg cattttacgc acccacgcag caatgagcgt
     7861 acttgggcat ggtttgctgc gcggcgcggg gctgggaggc cctgtgggcg cagcattaaa
     7921 tggggttaaa gcggtcagcg gcagcaccta tcgcgtgacg attccagccg tactgcaaat
     7981 cgcctgcctg cgccggatgg ttagcgccac tcaggtctga taccggaaac cggggaaaat
     8041 tttcattttc ccctcaaaag atcgtagaca ctgccccact ggctgattat tatgccgcgc
     8101 cctgaaaaca ctacagttat tcagggaaat tatttcacca ttcattcgat gatgattttt
     8161 gaggaattat gggcaacact aagttggcta atccggcacc gctgggcctg atgggcttcg
     8221 gcatgaccac cattctgctt aacctgcaca acgtgggtta tttcgctctg gacggtatta
     8281 ttcttgccat gggcattttc tacggcggca tcgcgcaaat ttttgctggt ctgctggagt
     8341 acaaaaaagg caacactttc ggtttaaccg cattcacctc ttacggttct ttctggctga
     8401 cgctggttgc gattctgctg atgccgaaac tgggtctgac cgatgcgcca aatgcacagt
     8461 tccttggtgt ctacctgggt ctgtggggcg tatttacgct gtttatgttc ttcggcactc
     8521 tgaaaggcgc gcgcgtcctg caattcgttt tctttagcct gaccgtgctg tttgccctgc
     8581 tggcgatcgg taacattgcc ggtaatgccg cgatcatcca ctttgccggc tggattggtc
     8641 tgatctgcgg tgccagcgca atctatctgg cgatgggtga agtgctgaac gagcagtttg
     8701 gtcgcaccgt tctgccgatt ggtgaatccc actaattgtc ttgagatccc cccgctcggg
     8761 gggatttttt tattcgctaa cgtcgcgtct tgcactcttc ggtctgaatg cagcaaccac
     8821 ttctggtgcc gtttcaacgt atggcccttc cagcaactga atgcagtacg gtacgctggc
     8881 aaaaatacca tgcaccacaa cgttaccctc agcgtccttc acaccttcca gcgtctcttt
     8941 aatagacttc ggctgaccgg gtaagttaag gatcagcgcc tgtttgcgaa tcacgcccac
     9001 ctgacgcgaa aggatcgcag ttggtacaaa atgcaggctg atctggcgca tctgttcacc
     9061 aaagccaggc atctcgcggt ccgctactgc cagcgtcgca tcgggcgtta cgtcacgacg
     9121 cgccgggcca gttccgcccg tggtgagcac cagatggcaa ctcatttcat ccaccagctc
     9181 acacaacgtt tgctcgatga tcgcctgctc atcggggatt aagcgggttt ccagttcaaa
     9241 cggcgtggtt agcgccgatg tcagccattc ttccagcgct ggaatgcctt tatcctgata
     9301 aacgccgctg gatgcgcgat cagagatgga aactaagcca atacgtaaag tattcatcaa
     9361 gatttttccg tttaaacaat ctcgttaaac agaatgatac actgcgaagg gagtgacaga
     9421 caggcttcag aagaggtagc gtgaccgact tcccggtcac gctaagaatg attacagcag
     9481 atcgccgatc attttttcca gtttttcctg gtcaatagca aacttacgga taccttccgc
     9541 cagtttatct actgccattg gatcctggtt gtgctgccac aggaactcgg actcagtgat
     9601 acgcgccgga cgcgctttca cttcgccggt gtaagacagt ttacgttcga tagccccttc
     9661 gctctccgcc agctctttca gcagtgccgg tgcgatggtc agacggtcac agcctgccag
     9721 ttccagaatt tcgccgatgt tacggaagct tgcgcccata accacggttt cataaccgtg
     9781 ctctttgtag tactggtaga tttcagatac agaaaccacg cccggatctt ctgccggagc
     9841 gtactctttc ttatcggtat tcgctttgta ccagtcaaga atacggccaa caaacggcga
     9901 gatcaggaac acgcccgcct ccgcacaagc acgagcctga gcgaaggaga acagcagggt
     9961 caggttacag ttgatacctt ctttttccag ctgttctgca gcacggatac cctgccaggt
    10021 agaagccagt ttgatcagaa tacgatcgtt gctaatacct gcatcgttgt agagtttgat
    10081 caggcgtttt gctttcgcaa ttgacgcttc agtgtcatag gaaagacgcg catcaacttc
    10141 agttgagata cggcccggaa ccagtttcag gatttccaga ccaatattta ctgccagttt
    10201 gtcggtcgcg tccacgatct gctgcgcgcg atcgttgctc tgctgtttcg cccaggcgac
    10261 agcatcatca atcaacttac ggtattccgg aatctgcgct gcgttaagaa tgagagaagg
    10321 gttggttgtg gcatcctgcg gttgatacag cttcattgcc gcgatgtccc cagtgtcggc
    10381 cactacggtg gtgtactgac gaagggaggt caatttgtcc gtcatgatag tatttctctt
    10441 taaacagctt gttaggggga tgtaaccggt ctgccctgat gatatcacga cgcaacgtca
    10501 gcgcaaccgc cgacaagacg gttatcgcca ggcgagactg tttcggattt ctgacactgc
    10561 gttgatatcg cccaccattt ttatacaaaa cctcatgtat gctacgcaga agttatcaag
    10621 tacctcgtag cgtatatact tcttaaacaa ttggtaacgt ttacacagga aagtcatcgc
    10681 gaccggcaat aagagggata tgcatgccag attttttctc ctttattaac agcgtccttt
    10741 ggggatcggt aatgatttac ctgctcttcg gcgcaggttg ttggttcact tttcgcaccg
    10801 gatttgtgca gtttcgctac atccgccagt ttggcaaaag tcttaaaaat agcattcatc
    10861 cacagccagg cggtttaacc tcatttcagt cattgtgtac cagtcttgcg gcgcgcgtgg
    10921 gtagcggcaa cctggccggc gttgcgctgg caattaccgc cggtggacca ggtgccgtct
    10981 tttggatgtg ggttgctgcg tttatcggca tggcgacctc gtttgccgaa tgttcccttg
    11041 cacaacttta taaagaacgt gacgtcaatg ggcagtttcg tggcggaccg gcatggtata
    11101 tggcgcgcgg gctggggatg cgctggatgg gcgttctgtt cgccgtcttt ttgctcatcg
    11161 cctacggcat aattttcagc ggagttcagg cgaacgccgt tgcccgagcc ctgagttttt
    11221 cttttgattt tcctccgctg gtgacgggca ttattctcgc tgtctttgct ctgctggcaa
    11281 tcactcgtgg tcttcatggc gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga
    11341 taatctgggt actgaccagc ctggtgattt gcgtaataaa tatcgggcaa cttccccacg
    11401 tcatttggtc tatttttgag agtgcttttg gctggcagga agcggcaggc ggcgcggcgg
    11461 gatatacctt aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg
    11521 tgggaatggg atcgacgcca aacgcggcag cggcagctgc atcctggcct ccgcatccgg
    11581 cagcacaagg gattgtccag atgattggca tttttatcga caccctggtc atctgtacgg
    11641 caagcgccat gctgatatta ctggcgggta acggcacaac ctacatgccg ctggaaggta
    11701 ttcagcttat ccagaaggcg atgcgggtgc taatgggttc ctagggtgct gaatttgtta
    11761 ccctcgtggt tattctgttt gccttcagct ccatcgttgc caactacatt tatgccgaaa
    11821 acaatctctt ctttttacgc ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca
    11881 ccttcgcaac ggtcatcggc ggcaccttgc taagccttcc gctgatgtgg caactggcag
    11941 atatcataat ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg
    12001 tggttcatac cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt
    12061 ttgatccgtt gcgttatccg gatatcggtc gccagctttc tccggacgcg tgggatgatg
    12121 tttcgcagga gtaatcacaa ctatcgatca actcattctc tttttttgct aaagtcggca
    12181 taaatttcct gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt
    12241 accaaagccc gttgaccacc acgcgctata cgctgccgga gctgttagac aattcccagc
    12301 agttgatcca tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca
    12361 gcgacaaact ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc
    12421 cggcgaatgc ccgccaggcg attctggcgt ttaaaggtga tgtctacacc ggcttgcagg
    12481 ccgaaacctt cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg
    12541 gcttgtatgg cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga
    12601 tccgtcttga gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca
    12661 acaagctgaa cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag
    12721 atgagtattt taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt
    12781 tcctcgatga gaagaacggc aaatttaaga tcatcagctt ctacgctaag aaagcacgcg
    12841 gtctgatgag tcgtttcatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt
    12901 ttaatagcga aggttacttc tttgatgaag attcctccag caatggcgaa ctggtgttta
    12961 aacgctacga gcagcgttaa gtttgtagtc aatgccggat gcggcgtgaa cgccttatcc
    13021 ggcctacaaa agcatgcaga ttcaatatat tgcagaatta acgtaggcct gataagacgc
    13081 gccagcgtcg catcaggcgc tgaatgccgg attgttaccc ggcatttgtc atttagcggt
    13141 gatgtttgcc tggaccatga ccgccgtgat gatcatgagg agctttctta tggtggcgcg
    13201 gcggtggcgg cggtccgtgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc
    13261 accagccgtg gtcgcgccag tgacctccat cccagtaata gccacgatta tcacgatcgc
    13321 ctatctgtaa ttttactgac gggactaacg taatttccgc agcctgtgct gccatgggag
    13381 cgaccagaac cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct
    13441 tttatcattg cccgttatgg gtcgatatcc ggagattacg cgatgagaac gacggttgtt
    13501 attctccgca atcctaatct ctaagttacc gcatttattg ataatttctc ctttttccct
    13561 gtcgtttttc tccataattt cttcataaaa aagccgggct gcataaaagc aaacccggcc
    13621 tgattgagat aatgaataga ttttactgat gattcatcat caatttacgc aacgcagaaa
    13681 aatcggcggg cagattatgt gaaagcaagg gtaaatcagc acgttctgcc agctcttttg
    13741 gcagatccaa cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg
    13801 cggtgccgag gaacaagcca tattcgcctg gattcaactg gtcacgcagc gcacgatacg
    13861 ctacggcagc gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt
    13921 gcgtggtttc atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc
    13981 ggaacaactc ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata
    14041 acgtcgcctg agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat
    14101 cgttcacgtt ggtcgcagca ataaaacgtt tcaccggcag accgaatgac ttcgccagca
    14161 gacccgccgt caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg
    14221 cctcctgcgg cagctgcgca acagcttcaa agtagtagca aatctgcgcc agcaaacggc
    14281 tgatgttaat cgagttagcc gagtttaacc ccagcgccac tttcagttct tcatcatcaa
    14341 acgcctgctt caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga
    14401 tattgccgcc caacgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat
    14461 agaggataac cactttcaca ttcggcaaac cgtagaaagc atgagccact gcagctccgg
    14521 tatcaccgga ggtcgcggtc agaatggtca ctggcttatc atccgcaata tgggtcagca
    14581 tttgtgccat aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt
    14641 ccagacaacc gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc
    14701 gcacgcgctc ttccaggatt tcctgtggga tttcatcacc aataaacgcc gagaggatct
    14761 tcgcactgcg ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt
    14821 ccggcaggtc gtgcggaaaa aacagcccct gatttttgcc caacccctgg gttacggctt
    14881 gcgcaaagct gacctgctcg ttgtgatctt tcagattgta gagtttcatt tagttttcca
    14941 gtactcgtgc gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca
    15001 ggtagttctt acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacatagag
    15061 cgaacaaggt cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcgaccg
    15121 cctgccgcgc ctgccggaag ccaggcagta accgttcacg gtagggttca gcgataacat
    15181 ctttcatcag cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagccag
    15241 ccagatgtcg cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc
    15301 gggcttctgc cgtcgagact ttaattcccg gatacgccag cacccacagc cactcatcaa
    15361 accctggcac ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa
    15421 gaaaacacgg tgccacgttg tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc
    15481 ccatcaaagc cagcaaacga gtgtcattaa gcggcttgcc gcagtgttca ttcatcgcca
    15541 tcagcgccgc gaccaccgaa caggcgctgg agcctaagcc cgaaccgatc ggcatattct
    15601 tttccagagt catcgccact ggaatttgct tgccaagctc ctggcaaaaa cgctcccagc
    15661 actgataaac gatattttcc cgtggttctg acggcagctt atcggcaaag cgtccgagtt
    15721 tgttgagact gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat
    15781 caacgggtgc caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag
    15841 ccggggcata aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga
    15901 tcggcaaaga caccggcagc ggtaacgtca ttgcccgcac catatccgcg cagcaccaac
    15961 ggcagcggct gataatagtg gctataaaag gccagggcgt tttcgccatt tttcactttg
    16021 aacagcggat cattcccatc cacttcggca atcttcacgc ggcagacacc atcttcatca
    16081 atattgccaa catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca
    16141 aagagatcgt cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcggcgtta
    16201 aactctgcgg gcagcacagg ttcaatttca atatccgcca gctccagttc acgtcccgtt
    16261 tcacgagcga gaatcaatag tttacgcgcc acatccatac cagaaagatc atctcgcggg
    16321 tccggttcgg tataacccat ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct
    16381 tcgtctaact tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcacc
    16441 aattcatcac ctgcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca
    16501 acgttggtgt catagaggaa tttacgccgc gatttttccg ccgcataacg caactgatga
    16561 tagtaatcca tcgacgaggt gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg
    16621 cgcaggaagt cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc
    16681 gggttcagca gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct
    16741 ttggcttgtg caagttcttc ctgccagttt tccagattaa ggccatgcac actagtgagc
    16801 agagccttcg agttggcaac gccgcagaca cgtaagtcga tatgtttatt cttcagccag
    16861 ctttgctgac gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca
    16921 aacacttcga taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg
    16981 gtcgcatcat cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg
    17041 gcgacaatgt tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc
    17101 aaggtgcgca taccatcacc taccaccgag ataatggcca gccgttccgt caccgccagc
    17161 ggctccagta agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagct
    17221 cgcacacagt cgctttgtgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta
    17281 atcagcacca cggaaatacg ggcgcgtgac attgcagcaa agacgcgcgc cgccatgcca
    17341 accatccctt tcatccccgg gccggaaacg ctgaacattg ccatgttatt cagattggaa
    17401 atgcccttga ccggtaattc gtcttcatca cggctggcac caatgagcgt acctggtgct
    17461 tgaggatttc cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg
    17521 gtgcgggggt gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac
    17581 atcgacttca acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca
    17641 acgtccgtcc aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag
    17701 tagtcggaac cgttacgtcc aagcaccacc agttcgcctt tttcattacc ggcggtgaaa
    17761 cctgccatca acaccatgtg atcagccgga atgcggcttg ccgcaatacg gcgggtggac
    17821 tcagcaatat cgacggtaga ttcgaggtaa tgccccactg ccagcagttt ttcgaccgga
    17881 tcgataacgg taacgttgtg accacgcgct tctaacacgc cggccataat ggcgatcgac
    17941 attttctcgc cacggcaaat cagcgcagcg ttgatgctat ccgggcactg ccccaacaaa
    18001 ctaatgccat gcaggacatg ttttatttgg gcaaattcct gatcgacgaa agttttcaat
    18061 tgcgccagcg ggaaccccgg ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata
    18121 cgttcggcat cgctgatatt gggtaaagca tcctggccgc taatggtttt ttcaatcatc
    18181 gccaccaggt ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc
    18241 ctggcattgc tttccagaat atcggcaaca cgcagaaaac gttctgcatt tgccactgat
    18301 gtaccgccga acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa
    18361 gcccgcactg tcaggtgcgg gcttttttct gtgtttcctg tacgcgtcag cccgcaccgt
    18421 tacctgtggt aatggtgatg gtggtggtaa tggtggtgct aatgcgtttc atggatgttg
    18481 tgtactctgt aatttttatc tgtttgtgcg ctatgcctat attggttaaa gtatttagtg
    18541 acctaagtca ataaaatttt aatttactca cggcaggtaa ccagttcaga agctgctatc
    18601 agacactctt tttttaatcc acacagagac atattgcccg ttgcagtcag aatgaaaagc
    18661 tgaaaaatac ttactaaggc gttttttatt tggtgatatt tttttcaata tcatgcagca
    18721 aacggtgcaa cattgccgtg tctcgttgct ctaaaagccc caggcgttgc tgtaaccagt
    18781 cgaccagttt tatgtcatct gccactgcca gagtcgtcag caatgccatg actcgttcgc
    18841 gtaaagcttg cagttgatgt tggtctgccg ttgtgtcact tttcgccggt tgttgtatta
    18901 atgttgctaa ttgatagcaa tagaccatca ccgcctgccc cagattgagc gaaggataat
    18961 ccgccaccat cggcacacca gtaagcacgt cagccaacgc taactcttcg ttagtcagcc
    19021 cggaatcttc gcgaccaaac accagcgcgg catggctcat ccatgaagat ttttcctcta
    19081 acagcggcac cagttctact ggcgtggcgt agtaatgata tttcgcccga ctacgcgcag
    19141 tggtggcgac agtgaaatcg acatcgtgta acgattcagc cagtgtcggg aaaactttaa
    19201 tattatcaat aatatcacca gatccatgtg cgacccagcg ggtggctggc tccaggtgtg
    19261 cctgactatc gacaatccgc agttcgctaa aacccatcgt tttcattgcc cgcgccgctg
    19321 ccccaatatt ttctgctctg gcgggtgcga ccaggataat cgttatacgc atattgccac
    19381 tcttcttgac caaataaccg cgaacagggt gatcactgtc aacttattac gcggtgcgaa
    19441 tttacaaatt cttaacgtaa gccgcagaaa aagcccttta cttagcttaa aaaaggctaa
    19501 actattttct gactgcacta acggtttaat tgttaaaaaa tgctacatat ccttctgttt
    19561 acttaggata attttataaa aaataaatct cgacaattgg attcaccacg tttattagtt
    19621 gtatgatgca actagttgga ttattaaaat aatgtgacga aagctagcat ttagatacga
    19681 tgatttcatc aaactgttaa cgtgctacaa ttgaacttga tatatgtcaa cgaagcgtag
    19741 ttttattggg tgtccggccc ctcttagcct gttatgttgc tgttaaaatg gttaggatga
    19801 cagccgtttt tgacactgtc gggtcctgag ggaaagtacc cacgaccaag ctaatgatgt
    19861 tgttgacgtt gatggaaagt gcatcaagaa cgcaattacg tactttagtc atgttacgcc
    19921 gatcatgtta atttgcagca tgcatcaggc aggtcaggga cttttgtact tcctgtttcg
    19981 atttagttgg caatttaggt agcaaacatg cagaccccgc acattcttat cgttgaagac
    20041 gagttggtaa cacgcaacac gttgaaaagt attttcgaag cggaaggcta tgatgttttc
    20101 gaagcgacag atggcgcgga aatgcatcag atcctctctg aatatgacat caacctggtg
    20161 atcatggata tcaatctgcc gggtaagaac ggtcttctgt tagcgcgtga actgcgcgag
    20221 caggcgaatg ttgcgttgat gttcctgact ggccgtgaca acgaagtcga taaaattctc
    20281 ggcctcgaaa tcggtgcaga tgactacatc accaaaccgt tcaacccgcg tgaactgacg
    20341 attcgtgcac gcaacctgct gtcccgtacc atgaatctgg gtactgtcag cgaagaacgt
    20401 cgtagcgttg aaagctacaa gttcaatggt tgggaactgg acatcaacag ccgttcgttg
    20461 atcggccctg atggcgagca gtacaagctg ccgcgcagcg agttccgcgc catgcttcac
    20521 ttctgtgaaa acccaggcaa aattcagtct cgtgctgaac tgctgaagaa aatgaccggc
    20581 cgtgagctga aaccgcacga ccgtactgta gacgtgacga tccgccgtat tcgtaaacat
    20641 ttcgaatcta cgccggatac gccggaaatc atcgccacca ttcacggtga aggttatcgc
    20701 ttctgcggtg atctggaaga ttaatcggct ttaccaccgt caaaaaaacg gcgcttttta
    20761 gcgccgtttt tatttttcaa ccttatttcc agatacgtaa ttcatcgtcc gttgtaactt
    20821 ctttactggc tttcattttc ggcagtgaaa acgcatacca gtctatatta cgagtcacaa
    20881 acatcatgcc ggccagcgcc accaccagca cactggttcc caacaacagc gcgctatcgg
    20941 cagagttgag cagtccccac atcactccat ccagcaacaa cagcgcgagg gtaaacaaca
    21001 tgctattgcg ccagcctttc aataccgcct gcaaataaat accgttcatt aacgccccaa
    21061 tcagactggc gattatccat gccacggtaa aaccgatatg ttcagaaagc gccagcaaga
    21121 gcaaataaaa catcaccaat gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt
    21181 gcgcggtgag cgtttcaaaa acaaagaacg ccataaaagt cagtgcaatc agcagaatgg
    21241 cgtacttagt cgcccggtcc gttaattggt attgatcggc tggcgtcgtc actgcgacgc
    21301 taaacgcggg gaagttttcc cagccggtat cattgcctga agcaaaacgc tcaccgagat
    21361 tattagcaaa ccagctgctt tgccattgcg cctgaaaacc tgactcgcta gcttcccgtt
    21421 tggctggtag aaaatcacct aaaaaactgg gatgcggcca gttgctggtt aaggtcattt
    21481 cgctattacg cccggcaggc accacagaaa gatcgccggt accgcttaaa ttcagtgcca
    21541 tattcagctg caggttctgc ttccgccagt ccccttcggg taaagggata tgcacgccct
    21601 gcccgccttg ctctaacccg gtaccgggtt caatggtcag tgccgttccg ttaacttcag
    21661 gcgctttcac cacaccaata ccacgcgcat ccccgacgct aatcacaata aagggcttgc
    21721 ctaagatgat atttggcgcg ttgagctcgc taagacgcga aacatcgaaa tcggctttta
    21781 gcgttaaatc actgtgccag acctgaccgg tataaatccc tatcttgcgt tcttccacgt
    21841 tctgattgcc atcaaccatc aatgactcag gtaaccaaaa atggataaaa cttcgtttcc
    21901 gctccacggt tttatcctct tcctgcaccg tataaagctc ggtcacagga atagcgataa
    21961 gcggcccaac gagtttttgc ggcccgctgg tactttggcg aatcgcatct tccacatcgc
    22021 tacggtaatc ggcacgttcg acaatcactt gccgaatcag cattatcgga atcaacaaca
    22081 gcaatactgc accaaacaag gtggtcattt tccagaacag gggggatttc aacatagtct
    22141 tctcctttgc aatggcagca ggatacgaag gctatgtggg aagaatttga agctatgtga
    22201 agtgacggtg aagtcgtagc gaggccagca cgccaccttc ctgcacgttg cgcagcgtga
    22261 cttcgccgtt aaacaaacgg gcgacctcac tgacgaacgc caaccccaga ccgctgcttt
    22321 tttgcccatt tgcacgcggc aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt
    22381 caggaatacc actaccggta tccagcacct taagcgtgac gtgttcctga tccacttcgg
    22441 cgcttagcgt tatgcaaccg ctctcgggag taaaatcgat ggcgttatcc agtaaattcc
    22501 ccagcgcctg atccagtaac gccggttcag cagcaacatt aacctcggtg ggcataacat
    22561 gcaaagtgat gtttttttct gccaactgca cggtgcgcgc ttcgctgacg cggcgaaata
    22621 atgccgccac atcaacagca gtcagaacga cttcctgacg attctccagt cttgcctggc
    22681 gtagtaacgt ttccaccagc gcctgcattc gcgcattttg tgtcagaatg ttgtcagtaa
    22741 aacgagccac tacttcaggc ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg
    22801 ccgccagtgg gctttttagc tcatgggtta acgcataaac atactgctca atatagtttt
    22861 tcccttccag cttcacgcgc atactttcca gcgcctgcgc gagtttacgc agctcgctac
    22921 taccgagatc gggaagagga acgggcttat tgtcagtgac ggaatcagca tagcgagtga
    22981 gccgggcaat ggagcggttg atccaccaaa ccatgcctgc gccaatcacc agtgcaatcc
    23041 ccaacaaaat ggcgctggcc cataaaattc gccgctcgct gcgtttaatg accggagcca
    23101 tcgccgcgtt cggtttgcct acgctcaaaa cgccaataag ccgcgagccg cccataatcg
    23161 gcgcggcaac atacatcacc gaactttcgg gatcggcagg attttgcaac gtgctgcgcg
    23221 caccatactg accacgcaac gttagccaga cgtcattcca gcgcgaataa tcctgtccaa
    23281 cggctttatt tgccgaatcg aacaatactt tgccatgcgc atcggtcata tagacatgat
    23341 attcattgcg cactttgtta atgccaccaa tattggcgcg aaacgggcga tgttgtagct
    23401 gattaaacgc ctgcgccagt tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg
    23461 ccagctccgc aagtaacgtt gccgtgtcga tcaacgtccc ctccgttgct cttcgcacgc
    23521 ccggtttaac ttctttgaca aaaatagcca gtacgaacca ggccgccacc gccaccagta
    23581 aaaaatagcc cagcagcaac cgcatgccga tacgcattac aagcccctca ggctatatcc
    23641 catgccgcga tgagtattaa tcggtgaaag atcggggttg atggcgcgca gcttggcacg
    23701 cagcgtttta atgtggatat cgacggtgcg atcgtaggtg tcctgcgcat cttcccacac
    23761 gctatccatt agttgctggc gggaccagac gcggcctggc gacttgagta acgtcttcag
    23821 caataaaaac tcataccgcg tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc
    23881 gggttcattc aattcaaaat gaccaatacg gatgacggga gacggcgtcg agaacttctt
    23941 cacccgacgc agtaaggtgc gcaccctggc gcacacttcg cggggtgaaa acggtttagc
    24001 cacatagtcg tcagcgccaa tttccagccc aagcaggcga tcgacctctt cacttcgggc
    24061 cgtcaggaac agtacaggta acgccggata gagcgccagt aactggcggc acaattcaaa
    24121 gccactgata tcaggcaaac caacgtcgag aatcatgacg tcgggaacct gctggcgagc
    24181 tttatccagc accggcaaac ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa
    24241 catgtagacc agcgtgtcgg ctatcccttg ctcatcttcc actaaccaga ccgtttcccg
    24301 ttgcataaat cgcctctgtt attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt
    24361 tttcggcgaa ccttctacaa ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt
    24421 ggcgtcataa aagcgcacga cctgtaacga cttaaagacc agggacgtgc gttttttgaa
    24481 tactacctcg ccctgagctt tgccgttttt aatacgatcc gacagttcaa tcggcccgac
    24541 ttgctgacaa gaaatggccg catcggaggt atcttctgcc aggcccaatc cccctttaat
    24601 accaccggtt ttcgcccggc tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa
    24661 ggcttccaca acaattttgt gatccgggcc gatcatttta aatacggtgt cgaccgaacc
    24721 aatctcttct gcatgagcca atggccccag cattatcagg cttaaagaca ggatcaaatg
    24781 cttgtatttc atattgttac catttttatc tattacacga gcaattagtt cgtcacggta
    24841 aataacatat tgattaagaa caaaatctca attcttttat ttccgtcaag ccctaaaaca
    24901 tactctacta agaaaaaaac actgaatgct aaaacagcaa aaaatgctat tatccaatta
    24961 cctgatgtca ggtgctcgtt gttgaaagga tgaggatatt ttatggatca ggccggcatt
    25021 attcgcgacc ttttaatctg gctggaaggt catctggatc agcccctgtc gctcgacaat
    25081 gtagcggcga aagcaggtta ttccaagtgg cacttacaga gaatgtttaa agatgtcact
    25141 ggccatgcta ttggcgcgta tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta
    25201 cgcctgactg cgcgtccgat tctggacatc gcgctgcaat accgcttcga ctctcaacag
    25261 acatttaccc gcgcattcaa gaagcagttt gcccagactc ctgcacttta ccgccgttct
    25321 cctgaatgga gcgcctttgg tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca
    25381 gagcacaaat ttgtcaccct ggaagatacg ccgctgattg gtgttaccca gagctactcc
    25441 tgttcgctgg agcaaatctc tgatttccgc catgaaatgc gttatcagtt ctggcacgat
    25501 tttctcggca acgcgccgac cattccgccg gtgctctacg gcctgaatga aacgcgtccg
    25561 agtcaggata aagacgacga acaagaggta ttctatacca ccgcgttagc ccaggatcag
    25621 gcagatggct atgtactgac ggggcatccg gtgatgctgc agggcggcga atatgtgatg
    25681 tttacctatg aaggtctggg aaccggcgtg caggagttta tcctgacggt atacggaacg
    25741 tgcatgccaa tgctcaatct gacgcgccgt aaaggtcagg atattgagcg atactacccg
    25801 gcagaagacg ccaaagcggg agatcgccca attaatctgc gctgtgaact gctgattcct
    25861 atccgccgtt aacgctgcag ctcatctaat gcaggggcgt ccagatgcga gatgtcccct
    25921 gcggtttcaa cgacccagcc agacgccagc cacaggcttt cctgataatc cacgcgcgaa
    25981 atagaacagt tacgcagacg taagcggcgc tctgcccatg ctggtaatcc gagaatcgta
    26041 ctcaccagac atcccagtgc aataccatgg ctcaccagca gcggtcggct tccctgcggt
    26101 aagtcccggc aagattccag tgccgcattg acgcgatcgc tgagctcctg cattgactcg
    26161 ccttcaggaa tacgcccgtc aacggtgcca ttgaccagct gccgacgcca gttctcttct
    26221 tcttcggtca gagaatcgat atgtcttttt tccagcacac ccatgtttaa ttcacgcagg
    26281 cgagaatcaa agatgatgtc acagccgcag gcctgggcga tgatttccgc cgtacgccgg
    26341 gtgcgtccta aatcgctact gataatatgc gtaatgccaa gctctttggc acgggttgcc
    26401 acctgcatcg cctgttgctc acctttggcg gtcagcgggc tgtcagactg gccctgaata
    26461 cgtcgctcgg cgttccactg cgtttcaccg tggcggacta ggtatacctg taacatgctt
    26521 tttttccgta tactgctgta acgttaattt tctgagtaat gctgattatg caccaagttg
    26581 tctgtgcgac caccaatccc gctaaaattc aggccattct gcaggcattt cacgagatct
    26641 tcggcgaagg atcctgccat attgcatccg ttgccgtcga gagcggtgta ccggaacaac
    26701 cctttggcag tgaggaaacg cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt
    26761 tacttccaga ggctgatttt tgggtggcga ttgaagctgg cattgatggc gacagcactt
    26821 tcagttgggt ggtgattgaa aacgccagcc agcgcggcga ggcgcgttcg gcaactttgc
    26881 cgttacctgc ggtgattctg gaaaaagttc gtgaaggcga ggcgcttggt cccgtgagtc
    26941 gcgttatacc ggtattgatg agattggtcg taaggaaggg gcgatcggcg tgtttaccgc
    27001 cgggaaactc actcgcgcca gcgtctatca ccaggcggtg atcctggcgc tgagtccgtt
    27061 tcacaatgcc gtgtatcagg cattgtagga cggataaggc gttcacgccg catccgacat
    27121 tcggtgcacg atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aatcaatcgc
    27181 ttttcagcaa cacctcttcc agccactgac gcagctccac gggcgcggct ttcaggctgt
    27241 tagatccacg cgtaatcgtc gcgatgcccg cgccgagttc attttttaac tcacgctgac
    27301 tcatttcgcc acgcaacagc tcttcgacaa tacgcacgcg agtccccaac gcttcgcgct
    27361 catctggcgt cagcatcagg tttaacaacg gtaaatggag atcgttttgg taggcattct
    27421 taagcaggtc gacaaaacgt aaccactcct ggtgacgctg ttctgccatc gctgctgaat
    27481 agggtgattg ttgggccata atatgtcgcc attgttagcg ggggaagcaa aatgactccc
    27541 ccggttgtac tcgctaaaga gtacgatagc atatcataaa cgtgcggatc agtaacgacg
    27601 tccccattcc gtggcgctca tcaacgtcgg tttatccccc atgaaatagc ggtagtaagc
    27661 atcataagcc agcacgttct tcacataacc gcgcgtctcg gagaacggaa tactctcgac
    27721 aaatgccaca gcgtcgatac gcccggcgct gttgccaagc caggttcgca cccgtccagg
    27781 tccggcgtta taagctgctg aggagaaaat acggttattg ccaaactgct gataaacata
    27841 ttgcaggtaa ctggtgccaa tgttgatgtt cgtttccgga tccagcaatt gcccagggct
    27901 gctataacca ggaatagaga acatcttcac cgtatgggtc gctgtaccag gcataatctg
    27961 catcaggccg ctggccccta ccggtgattt cactttcgga ttccaggcgc tctcctgacg
    28021 ggcaatcgcc atcgcatagc tttgcgggat ctctttccca ctggtgtaac gtttgaaaag
    28081 atcgttgtaa gccagcggga atcgctcttc cagatgatcc caaagcttcc cggcgatcgt
    28141 tgcctgaacg ctaagatccc accattggtt attgaaagca taccgagcca gttgagcctg
    28201 ctctgttttt gacttgctct tcaccagatt ggcccactcg ctacgcgcgg tattatcgag
    28261 attccagtac atcaactcgc gcacgcgcgc catctccggc ccctgagtca gggtgctgtc
    28321 aacattctgc ggcgctttat caatcttcag ctcatactct tcaccgatgc gttgtgctgc
    28381 aaccatcggg tagaaaccac gctgttgtat gagttgatgc aaaatctctt tcgcttcagc
    28441 ttcacgtccg cgttccagca ataaatccgc ctgccagtaa cgccattcat ctttctcttt
    28501 tgcttccatc ggcagacgcg ccagccaggt attcaggccg cgacgatcgc cggtgccaag
    28561 cgccattcgc acacggcgtt caatcaacga cgtggattgc gagcgcataa tggcgtcatc
    28621 gcgccatttc gcctgctcat cggtgacatc attgcccatc aaacgccagg cgacgatatc
    28681 gcgcagctcc tgaatctgat cttcattaag ctgctgcgcc tgggcaagcg acgggatcat
    28741 cagccgtgca ttttcggcat cctgccgcgc cacactggca aacgccaccg ccgccatttg
    28801 acgggtaaaa tcggtcgcgc cggttgtacg cgcgaaggtc agtaccgtat tagggttgtt
    28861 cgccagtgaa atgattgccg aggcgatagt ctggtaatcg gcaggcatct gccctgccag
    28921 cactgttacc aggcctgtgt tacccgcttt catcgccaga cggatacgct ctaaatacgc
    28981 cagcggatct tgtttacctg acgcacgcca gacgctaaat aacttgtcac aggcgttagg
    29041 ctggctcttg ccggttagcc acagctcttt cgccccttgc caggcttctt cactctgccc
    29101 ggtgttccat ttcgcatagt agtaattaca ttgcgcttcg gtagttccgg gcttttccgg
    29161 gctaaaggct aacaagccac gccagtcttc acgccgcgcc agttcattga cgaaacgaga
    29221 ttgcagcgtg cgagcgggag gaagcgtggg gttagcgcga acaaagttag tgaccgtcac
    29281 cgccggttga ttcatcaggt catcggtgat ctggcggtat tccaggtagg gataaagcgg
    29341 ataatccttc agtccaggca tcatttgttc gaccacatcc atctgtcgat tatcccaggc
    29401 ctgcttgatt tgcgcgtaac ggctacgctg ctcatccagt gagtcggctc gcgccacgct
    29461 gctgaccgtc agcagacaga caccggcagc caacagccgc caggtaactt gtttggcttt
    29521 ttccacaagc gcatcctcta agtgtaaata catcaatgca gcgtcatgcc gcgtaataga
    29581 tttatgctaa ccagtcattg ccgtttacgc cacgttacgg acactttttt acttttactg
    29641 cgaaggtgat cgaccaactg ggattaggcg gggaaacagg ctacacttcg cctttgaaaa
    29701 ctcatcatca cgataaacat agaggcgaag tccaacgtgg ctcaattcgt ttataccatg
    29761 catcgtgtcg gcaaagttgt tccgccgaaa cgtcatattt tgaaaaacat ctctctgagt
    29821 ttcttccctg gggcaaaaat tggtgtcctg ggtctgaacg gcgcgggtaa gtccaccctg
    29881 ctgcgcatta tggcgggcat tgataaagac atcgaaggtg aagcgcgtcc gcagccagac
    29941 atcaagattg gttatctgcc gcaggaaccg cagctgaacc cggaacacac cgtgcgtgag
    30001 tccattgaag aagcggtttc agaagtggtt aacgccctga aacgcctgga tgaagtgtat
    30061 gcgctgtacg ccgatccgga tgccgatttt gacaagctgg ccgctgaaca aggccgtctg
    30121 gaagagatca ttcaggctca cgacggtcat aacctgaacg tacagctgga gcgtgcggcg
    30181 gatgcgctgc gtctgccaga ttgggacgcg aaaatcgcta acctctccgg tggtgagcgt
    30241 cgtcgcgtgg cgttgtgccg cctgctgctg gaaaaaccag acatgctgct gctcgacgaa
    30301 ccgaccaacc acctggatgc cgaatccgtg gcgtggctgg aacgcttcct gcacgacttc
    30361 gaaggcaccg tggtggcgat tactcacgac cgttacttcc tcgataacgt tgcaggctgg
    30421 atcctcgaac tggaccgcgg tgaaggaatt ccgtgggaag gtaactactc ctcctggctg
    30481 gagcagaaag atcagcgtct ggcgcaggaa gcttcacaag aagcggcgcg tcgtaagtcg
    30541 atcgagaaag agctggagtg ggtacgtcag ggaactaaag gccgccagtc gaaaggtaaa
    30601 gcacgtctgg cacgctttga agagctgaac agcaccgaat accagaaacg taacgaaacc
    30661 aacgaactgt ttattccacc tggaccgcgt ctgggcgata aagtgctgga agtcagcaac
    30721 ctgcgtaaat cctacggcga tcgcctgctg attgatgacc tgagcttctc gatcccgaaa
    30781 ggggcgatcg tcgggatcat cggtccgaac ggcgcgggta aatcgaccct gttccgtatg
    30841 atctccggtc aggaacagcc ggacagcggc accatcactt tgggtgaaac ggtgaaactg
    30901 gcatcggttg atcagttccg tgactcaatg gataacagca aaaccgtttg ggaagaagtt
    30961 tccggcgggc tggatattat gaagatcggc aacaccgaga tgccaagccg cgcctacgtt
    31021 ggccgcttta actttaaagg ggttgatcag ggtaaacgtg ttggcgaact ttccggtggt
    31081 gagcgcggtc gtctgcatct ggcgaagctg ctgcaggttg gcggcaacat gctgctgctc
    31141 gacgaaccaa ccaacgacct ggatatcgaa accctgcgcg cgctggaaaa cgccctgctg
    31201 gagttcccgg gctgtgcgat ggttatctcg cacgaccgtt ggttcctcga ccgtatcgcc
    31261 acgcacatcc tggactacca ggatgaaggt aaagttgagt tcttcgaagg taactttact
    31321 gagtacgaag agtacaaaaa acgcacgctg ggcgcagacg cgctggagcc gaagcgtatc
    31381 aagtacaagc gtattgcgaa gtaatgcgta aaatgccgga tgcggcgcga acgccttatc
    31441 cggcctacaa aaccagcaat ttcaatacgt tgtaaaactg taggcctgat aagacgcggt
    31501 aagcgtcgca tcaggcattg agcaccgaat gccggatgcg gcgtgaacac cttttccggt
    31561 ctacaacagc cttagcatcc tcaatttcgt ggcgggtcac ccgtgcaggt tttttcaaac
    31621 cttgtttaat ctcgacagcc tcttccagag aggttttcaa ctcatcaaaa aaactcattt
    31681 tatacctcgc ctttgagcag tttcgtcacg gttatccttg ctcccccatc atctcccgca
    31741 ccagctccac gcagcgcagg aaacggctgt cgtaatcgtc ctcttcaacc cgcacgaatt
    31801 cgatattgtt ctcttccagc atctccacca gcaagttctg gaactctttg cgatccaccg
    31861 aactgccgag gctgcgtaaa ccatccgcca cccacggcgt gttgttctcc agcaggatca
    31921 ccagatcgaa acggtattca tcaatcagcg cctgcacgaa cggatgctca cgcccttcgt
    31981 actttttgca gaacgcctgg gtagtgacaa aatcggtgtc aataaacgcc actttattgg
    32041 cgtatttcac tgcaaaatca atgtattgtg cgtgacccaa cgcgatttta tcgtagtcgg
    32101 aatactgcaa tgcgatttca tcaccgccga ggtgtgaaaa gacataatcg cggccatatt
    32161 cccacgcact ggtggtgttg aagatattgg caagtttgtt taccagggtg gatttaccgc
    32221 tcgactcgcc gccaaggatc gccacggtac gcacaaaaaa cggcttcact tcggtaggaa
    32281 tatattccca gtagcggaac gggttttcgc ggatctgcgc accgctgata ctcataaagg
    32341 tacgtttcgg atcgaccagc accgtctcga tccccagatg ttccatatac tgtggcgcat
    32401 cggcttcttc cgaggtgtag atcaggtccg gctggatccc cttttcagcc ataaactttt
    32461 tgatgccgtt gctccacaca tcccagccat gcggatacgg ctccatgccc tcttcgttga
    32521 aagcatgaat acggatattt ttctgatatt taaaggtttg caatagccag cgcagacgat
    32581 ccggcaccgt gggctgctgc gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat
    32641 cgtcaaaacc cataatgata tgcagctcgt caacctggct acaggcgcgc tggataaggt
    32701 agatatgtcc ggtatgcagc gggtagaact taccgaatac gacaccaatg gttttcttct
    32761 gccggggaaa ttcaagcccc aaaaaacggt gcaatgcctc cagcttttgc gcgctggggc
    32821 ttttaatttt ggcattcagt aactggctta aatacccttt ggtcataccg ctggcatcag
    32881 ctacctgctg tagcgtgcag ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg
    32941 acatatctcc ctccgtatct ctcattataa gtcgtcgaac acgctaagcg cgtcggagag
    33001 ttttttaacg ccaaaaatct gcatcccttc cggcgctttt ttcggtacgt tagccgccgg
    33061 aacaatcgcc cggcgaaaac cgtgtttcgc cgcttcagaa atccgctcct ggccactggg
    33121 caccgggcgg atctcccctg ccagcccgac ttcaccaaac accaccagat cctgtggcag
    33181 cggtctgtca cgcaggctgg aaaccatcgc cagcagtaac gctaagtcgg cgctggtttc
    33241 ggttaccttc acgccgccga ccacgttcac aaacacatcc tgatcggcca tttgcagacc
    33301 accgtgacgg tgcaagacag ccagcaggat tgccagacgg ttttgttcca gccccactgc
    33361 cacgcggcgc ggattcgcca ttatcgagtg atcgaccagc gcctgaatct ccaccagcaa
    33421 cggacgcgtt ccttcccaca ccaccatcac ggagctgccg gaggtcactt catctccgcg
    33481 acttaagaaa attgccgaag ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa
    33541 gacgcccagc tcattcaccg cgccgaagcg gtttttatgg ctgcgtaagg tgcgaaaacg
    33601 ggagtcggca tcgccatcca gaagcaccga acagtcgata cagtgttcca gcactttcgg
    33661 gccagccagc gaaccatctt tggttacgtg tccaaccatg acaatcgcca caccgcgcgt
    33721 tttggcgaaa cgcgtcagat aagccgccgt ttcacgcacc tgcgccacgc taccaggcga
    33781 tgattgtaca tccgccatat gcatcacctg gatcgagtca attaccatca gcttcggttg
    33841 ctcttcttcg gcaatcaggc agatctgctc gatgctggtt tccgacaaca tattgagatt
    33901 gtcagtcggc aggccaaggc gatgagcgcg cattgccacc tgttgcagcg actcttcgcc
    33961 ggtgacatac agcgttttca tctgctgggc cagtttgcac agcgtttgca gcaacagcgt
    34021 ggatttcccc gcaccagggt taccgccaat cagaatggca cttcctggca ccacgccgcc
    34081 tcctagtacg cggtcgaact ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct
    34141 gatatcggag agtttctgga cttttgccac cccggcgcta ccggcatagc cgctgagacg
    34201 ctcgttacgc gccaccgttg gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc
    34261 atgacaggca ctgcactgcc cctgccagcg cggataatcg gccccgcatt cattacaaac
    34321 aaaggcgcgt tttggagctt ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg
    34381 atgacgggca ggcaattact tctgattcag gctgccagag aggatgcaga atacccccat
    34441 caggtcagcg tgacggatgg tgacttccgc cttttcattc acttttggct tggcatggta
    34501 ggcaatcccc agccctgctg ctttgatcat cggcaggtca ttagctccat cgccaatcgc
    34561 cacggtctgc gccagcggga tttcatactc ctgcgcgagg cgagtcagag ttttcgcttt
    34621 gtactgcgcg tctacgatgt cgccgatcac attgccggta aatttaccgt ccatgatctc
    34681 cagttcattg gctaccacgg ctgtcaggcg cagcttgtcg cgcaggtatt cagcaaagaa
    34741 agtaaagccg ccggaggcaa tcgccacttt ccagcccagc gtttccagct tgagcaccag
    34801 ctgcgttaag cctggcatca gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc
    34861 agcgcctttc agcgtcgcca cgcggctgcg caggctggcg gtaaaatcga gttcgccgcg
    34921 catcgcccgt tcggttacct ccgccaccat ctcgcccgtt ccggccagct tggcaatttc
    34981 atcaatacat tcaatctgga tggcggtgga atccatgtcc atcaccagca aacccggcgt
    35041 gcgcaggtgc gggattttcc ccagcggggc gacatccagc tgcgcttcgt gggccaggcg
    35101 tgtagcccgt gcggtgagtg aacctgccag acgaatcacc tgataatctt ccacgcacca
    35161 ggcggcaaca atcaccatcg ccgcacccag tttgctctgg tattgggtca gacgctgttt
    35221 atccagccca cgaccataca gcagccagcc gctacgacct gcgtggtaat ccagtggcat
    35281 cacttcatca ccacttaatg aaagaggcag acccggccat aaagagacat cttcaggcag
    35341 gtcgcaccag gtaatgttag gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat
    35401 aacgcatgag gctaccttgt atccattgct tctggcaaca ttaagtctca aattttcaaa
    35461 gggtggaaga tggctcgcac aaaactgaaa ttccggctac atcgggcagt gattgtcctg
    35521 ttctgtcttg ccttgttagt ggcactgatg cagggggcgt catggtttag tcaaaaccac
    35581 cagcgacagc gtaatccaca gctggaagaa ctggcccgca ccctggcgcg tcaggtgacg
    35641 ctgaacgttg caccgctgat gcgtaccgac tcaccggatg aaaaacgcat tcaggcgatc
    35701 ctcgatcagt taacagatga aagccgtatc ctcgacgcgg gtgtgtatga cgaacaaggc
    35761 gatcttatcg cacgttctgg cgaaagcgtc gaagtgcgcg atcggctggc gctcgacggt
    35821 aaaaaagcag gcggctattt taaccagcag attgtcgagc caattgcggg taaaaacgga
    35881 ccgctcggct atctgcgcct gacactcgac acccatacgc tcgccaccga agcccaacag
    35941 gtggataaca ccactaacat tttacgcctg atgttgctgc tctcactggc aatcggcgta
    36001 gtgctgaccc gcacgctgct acagggtaaa cgcacccgct ggcagcaatc gcccttcctg
    36061 ttaaccgcca gcaaaccggt gccggaagag gaagaaagcg agaaaaaaga gtgacccatt
    36121 actacaagaa aggaaatcgt tatgtccaca ttacgcctgc tcatctctga ctcttacgac
    36181 ccgtggttta acctggcggt ggaagagtgt atttttcgcc aaatgcccgc cacgcagcgc
    36241 gttctgtttc tctggcgcaa tgccgacacg gtagtaattg gtcgcgcgca gaacccgtgg
    36301 aaagagtgta atacccggcg gatggaagaa gataacgtcc gcctggcgcg gcgcagtggc
    36361 ggtggcggcg cggtgttcca cgatctcggc aatacctgct ttacctttat ggctggcaag
    36421 ccggagtacg ataaaactat ctccacgtcg attgtgctca atgcgctgaa cgcgctcggc
    36481 gtcagcgccg aagcgtccgg acgtaacgat ctggtggtga aaaccgtcga aggcgaccgc
    36541 aaagtctcag gctcggccta tcgcgaaacc aaagatcgcg gcttccacca cggcaccttg
    36601 ctactcaatg ccgacctcag ccgcctggca aactatctca atccggataa aaagaaactg
    36661 gcggcgaaag gcattacttc agtgcgttcc cgcgtgacca acctcaccga gctgctgccg
    36721 gggctcacca tgagcaggtt tgcgaggcca taaccgaggc ctttttcgcc cattatggcg
    36781 agcacgtgga agcggaaatc atctccccgg acaaaacgcc agacttgcca aacttcgccg
    36841 aaacctttgc ccgtcagagt agctgagagt ggaacttcgg tcaggctccg gcattctcgc
    36901 atctgctgga tgaacgcttt agctggggcg gcgtggaact gcatttcgac gttgaaaaag
    36961 gccatatcac ccgcgcccag gtatttaccg acagcctcaa ccccgcgccg ctggaagccc
    37021 tcgccgggcg actgcaaggc tgcctgtacc gcgcggatat gctgcaacaa gagtgcgaag
    37081 cgctgttggt tgacttcccg gaccaggaaa aagagctacg ggagttgtcg acgtggatag
    37141 cgggggcggt aaggtaactc gctgattaaa atatccaagg aaggaaatat gatcgatctg
    37201 gaaaaccagg aacgggagat tatcaattta atgttcagcc agcgtatttc ctggctagcc
    37261 gccgtgcgta tcaggcataa attatcgctt gctgaagtaa gtaaaatgtt aggtatctcg
    37321 attaattctc taaaacagat cgaaaagacg gaacggctta gctcaaacat caaaagcaaa
    37381 atggcagaaa tttatggttg tccaccagag ttgctgatat gcccttcct
//
LOCUS       DAZSIO010000022        36726 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZSIO010000022 DAZSIO010000000
VERSION     DAZSIO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.022"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            38..394
                     /locus_tag="ACHX5T_001001"
     CDS             38..394
                     /locus_tag="ACHX5T_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR8466293.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            398..1525
                     /gene="dapE"
                     /locus_tag="ACHX5T_001002"
     CDS             398..1525
                     /gene="dapE"
                     /locus_tag="ACHX5T_001002"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR8466294.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            1553..1753
                     /locus_tag="ACHX5T_001003"
     CDS             1553..1753
                     /locus_tag="ACHX5T_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR8466295.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(1863..2561)
                     /gene="ypfH"
                     /locus_tag="ACHX5T_001004"
     CDS             complement(1863..2561)
                     /gene="ypfH"
                     /locus_tag="ACHX5T_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR8466296.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(2635..4650)
                     /gene="tmcA"
                     /locus_tag="ACHX5T_001005"
     CDS             complement(2635..4650)
                     /gene="tmcA"
                     /locus_tag="ACHX5T_001005"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR8466297.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(4665..5528)
                     /locus_tag="ACHX5T_001006"
     CDS             complement(4665..5528)
                     /locus_tag="ACHX5T_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR8466298.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(5696..6409)
                     /gene="purC"
                     /locus_tag="ACHX5T_001007"
     CDS             complement(5696..6409)
                     /gene="purC"
                     /locus_tag="ACHX5T_001007"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR8466299.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(6622..7656)
                     /gene="bamC"
                     /locus_tag="ACHX5T_001008"
     CDS             complement(6622..7656)
                     /gene="bamC"
                     /locus_tag="ACHX5T_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR8466300.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(7673..8551)
                     /gene="dapA"
                     /locus_tag="ACHX5T_001009"
     CDS             complement(7673..8551)
                     /gene="dapA"
                     /locus_tag="ACHX5T_001009"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR8466301.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            8697..9269
                     /locus_tag="ACHX5T_001010"
     CDS             8697..9269
                     /locus_tag="ACHX5T_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR8466302.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            9269..9739
                     /gene="bcp"
                     /locus_tag="ACHX5T_001011"
     CDS             9269..9739
                     /gene="bcp"
                     /locus_tag="ACHX5T_001011"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR8466303.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            9992..10609
                     /gene="hyfA"
                     /locus_tag="ACHX5T_001012"
     CDS             9992..10609
                     /gene="hyfA"
                     /locus_tag="ACHX5T_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR8466304.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            10609..12626
                     /gene="hyfB"
                     /locus_tag="ACHX5T_001013"
                     /pseudo
     CDS             10609..12626
                     /gene="hyfB"
                     /locus_tag="ACHX5T_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            12637..13584
                     /gene="hyfC"
                     /locus_tag="ACHX5T_001014"
     CDS             12637..13584
                     /gene="hyfC"
                     /locus_tag="ACHX5T_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR8466305.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            13601..15040
                     /gene="hyfD"
                     /locus_tag="ACHX5T_001015"
     CDS             13601..15040
                     /gene="hyfD"
                     /locus_tag="ACHX5T_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFR8466306.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            15052..15702
                     /gene="hyfE"
                     /locus_tag="ACHX5T_001016"
     CDS             15052..15702
                     /gene="hyfE"
                     /locus_tag="ACHX5T_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR8466307.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            15707..17299
                     /gene="hyfF"
                     /locus_tag="ACHX5T_001017"
     CDS             15707..17299
                     /gene="hyfF"
                     /locus_tag="ACHX5T_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR8466308.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMA
                     KPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQ
                     RSTWHDFLPSGTASVSEKHSER"
     gene            17289..19003
                     /gene="hyfG"
                     /locus_tag="ACHX5T_001018"
                     /pseudo
     CDS             17289..19003
                     /gene="hyfG"
                     /locus_tag="ACHX5T_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            19013..19558
                     /gene="hyfH"
                     /locus_tag="ACHX5T_001019"
     CDS             19013..19558
                     /gene="hyfH"
                     /locus_tag="ACHX5T_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR8466309.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            19555..20313
                     /gene="hyfI"
                     /locus_tag="ACHX5T_001020"
     CDS             19555..20313
                     /gene="hyfI"
                     /locus_tag="ACHX5T_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR8466310.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            20306..20719
                     /gene="hyfJ"
                     /locus_tag="ACHX5T_001021"
     CDS             20306..20719
                     /gene="hyfJ"
                     /locus_tag="ACHX5T_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR8466311.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            20749..22761
                     /gene="hyfR"
                     /locus_tag="ACHX5T_001022"
                     /pseudo
     CDS             20749..22761
                     /gene="hyfR"
                     /locus_tag="ACHX5T_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            22783..23655
                     /gene="focB"
                     /locus_tag="ACHX5T_001023"
                     /pseudo
     CDS             22783..23655
                     /gene="focB"
                     /locus_tag="ACHX5T_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
     gene            complement(23749..24810)
                     /locus_tag="ACHX5T_001024"
     CDS             complement(23749..24810)
                     /locus_tag="ACHX5T_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR8466312.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            25023..26486
                     /gene="bepA"
                     /locus_tag="ACHX5T_001025"
     CDS             25023..26486
                     /gene="bepA"
                     /locus_tag="ACHX5T_001025"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR8466313.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            26507..26866
                     /gene="arsC"
                     /locus_tag="ACHX5T_001026"
     CDS             26507..26866
                     /gene="arsC"
                     /locus_tag="ACHX5T_001026"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR8466314.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(26999..27700)
                     /locus_tag="ACHX5T_001027"
     CDS             complement(26999..27700)
                     /locus_tag="ACHX5T_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR8466315.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(27795..29084)
                     /gene="uraA"
                     /locus_tag="ACHX5T_001028"
     CDS             complement(27795..29084)
                     /gene="uraA"
                     /locus_tag="ACHX5T_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR8466316.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(29170..29796)
                     /gene="upp"
                     /locus_tag="ACHX5T_001029"
     CDS             complement(29170..29796)
                     /gene="upp"
                     /locus_tag="ACHX5T_001029"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR8466317.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            30120..31157
                     /gene="purM"
                     /locus_tag="ACHX5T_001030"
     CDS             30120..31157
                     /gene="purM"
                     /locus_tag="ACHX5T_001030"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR8466318.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            31157..31795
                     /gene="purN"
                     /locus_tag="ACHX5T_001031"
     CDS             31157..31795
                     /gene="purN"
                     /locus_tag="ACHX5T_001031"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR8466319.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            31967..34033
                     /gene="ppk1"
                     /locus_tag="ACHX5T_001032"
     CDS             31967..34033
                     /gene="ppk1"
                     /locus_tag="ACHX5T_001032"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR8466320.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            34038..35579
                     /gene="ppx"
                     /locus_tag="ACHX5T_001033"
     CDS             34038..35579
                     /gene="ppx"
                     /locus_tag="ACHX5T_001033"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR8466321.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(35618..>36726)
                     /gene="pdeF"
                     /locus_tag="ACHX5T_001034"
     CDS             complement(35618..>36726)
                     /gene="pdeF"
                     /locus_tag="ACHX5T_001034"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR8466322.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT         8501 a         9714 c         9555 g         8956 t
ORIGIN      
        1 tgagtattta ttctcataaa tcgaaaaagg attcattatg gttacacttt acggcatcaa
       61 aaattgtgac accattaaaa aggctcgccg ttggctggaa gccaataaca tcgactatcg
      121 ttttcatgat taccgcgtcg atgggctgga cagcgaatta ttgaacggtt ttatcaacga
      181 attaggctgg gaagcgttac tcaacacccg tggtacaacc tggcgtaaac tggacgaaac
      241 tacccgcaat aaaatcaccg atgcggcctc tgcggcggca ttaatgactg aaatgcctgc
      301 aattatcaaa cgtccattgc tctgcgcgcc tggtaagcct atgctgctgg gtttcagtga
      361 ttccagttat cagcaatttt tccatgaggt gtagtctatg tcgtgcccgg ttattgagct
      421 gacacaacag cttattcgcc gcccttccct gagtcctgat gacgcaggat gtcaggcttt
      481 gttgattgaa cgtttgcagg cgatcggttt taccgttgaa cgcatggatt ttgccgatac
      541 gcagaatttt tgggcatggc gtgggcaagg tgaaacgtta gcctttgccg ggcataccga
      601 cgtggtgccg cctggcgacg ccgatcgttg gatcaatccg ccgtttgaac ccaccattcg
      661 tgacggcatg ttattcgggc gcggtgcggc agatatgaaa ggctcgctgg cggcgatggt
      721 ggtagcagca gaacgttttg tcgcacaaca tcccaaccat acggggcgac tggcatttct
      781 gatcacttct gatgaagaag ccagtgccca caacggtacg gtaaaagtcg tcgaggcgtt
      841 aatggcacgt aatgagcgtc tcgattactg cctggtcggc gaaccgtcga gtatcgaagt
      901 ggtaggtgat gtggtgaaaa atggtcgtcg cggatcgtta acctgcaacc tcaccattca
      961 tggcgttcag gggcatgttg cctacccaca tctggctgac aatccggtac atcgcgcagc
     1021 acctttcctt aatgaattag tggctattga gtgggatcag ggcaatgaat tcttcccggc
     1081 gaccagtatg cagattgcca atattcaggc gggaacgggc agtaacaatg ttattccggg
     1141 tgaactgttt gtgcagttta acttccgctt cagcaccgaa ctgactgatg agatgatcaa
     1201 agcgcaggta cttgccctgc ttgaaaaaca tcaactgcgc tatacggtgg attggtggct
     1261 ttccgggcag ccatttttga ccgcgcgcgg taaactggtg gatgcggtcg ttaacgcggt
     1321 tgagcactat aatgaaatta aaccgcagct actgaccaca ggcggaacgt ccgacgggcg
     1381 ctttattgct cgcatggggg cgcaggtggt ggaactcggg ccggtcaatg ccactattca
     1441 taaaattaat gaatgtgtga acgctgccga cctgcagcta cttgcccgta tgtatcaacg
     1501 tatcatggaa cagctcgtcg cgtgatgagt ggttctgcaa gaggaaataa gcatggactg
     1561 gctggctaaa tattggtgga ttctggtgat tgtctttttg gtaggcgtcc tgctgaacgt
     1621 gattaaagat ctcaagcgcg tcgaccataa gaaatttctc gccaacaagc cggagcttcc
     1681 cccgcatcgt gatttcaacg ataagtggga cgatgacgac gactggccga aaaaggatca
     1741 accgaagaag taagctgagt gctcaaccgt aggccggata aggcgttaac gccgcatccg
     1801 gcaatggtga acgatccctg atgcgacgct cacgcgtctt atcaggccag gtaatctgtc
     1861 ttttacatca tttcaatcac atcatcatcg ccaggcttac cgccacttag cgcttggtcg
     1921 aaataatgtt tcggaatggt ataacgcaag tgatccaggg caaattgcat actgcgattg
     1981 tcgatcgcat ggcctaaatc ctccacgata tccagcgtca cgtcaccacc ggcactgatt
     2041 aacgcttcct gcgccgcaac tgcatgcgcc agatcgataa ccggatcttc accaccatga
     2101 atcaagtgaa ttgtcgtggc tgtagacgcg gtctccggca ggctcgcata gcgtccgtta
     2161 aaggcaatga cgcgcgaagc aaggcccggt tcggctttaa tgctctccag cgccataatc
     2221 gcgccttgtg agaaaccaat tagcgccgtg gcgttagccc ctactccgct ctgtttctgc
     2281 cagtagcgta ccgtttcaat aaacgtcggc atgatggcat ccacacgcgc ctggcgatta
     2341 tcttccgtaa taccctgcac tgaaaaccac tgacgccccg ccggattacc gctcggctcc
     2401 gcgccaccga cactcaccac cagcgcatca ggaaacagcg gtgcaaacca gctgcctatt
     2461 tcccccattg ccaccgggtt atccccgaca ccgtggaaaa gcagtaacaa ctgttgtgca
     2521 ggtttatccg ggctttgaac aacaaaatgg tcatgtttca tggcgaactc cttaactgat
     2581 gtcattaatt ttacgccgct gactcacaat gaccatggga gaaaattgaa ggagtcagtg
     2641 aaaaaattgc cattgcgtta tgcgatcgcg cagacgctca gtgcgaacct catttagtgc
     2701 gaacagcgcc tgcgcggcct cttcccgctg gcgaaccagt aacatcttgc ggcctgaaag
     2761 tttaagtgtg gtacataact gcgcgtcact ggcgtttttc tgtaaacgcc cacgcagcgc
     2821 cggtaatgcc agttcactgg tttgtagcag acgcaataag caacctaacg atgttaatag
     2881 cggacgatga gcgaaagcaa aaccggccag ttcaagccag tcgtcgtcag aaaggatggc
     2941 atcgtttagt ggatcaacag gaagcatttc gccattccac tgcgcgagag cttgcgcatc
     3001 gcgacgtaaa cggtaatgct cacgttcagc cagctgttta cccgcatcac tcatcggtaa
     3061 cagcgccatc gccgtatagc aaccgctgct ggcttcccga tgattaccca tccgcaccag
     3121 cacaaaaccg cagcgttgcc agaaacgcca taactcccca gtgtaaccaa aactcaccga
     3181 aagatagtcg aggtcatgcg tatattgcaa agctccagca ataagttgtc gccctgtgcc
     3241 ttcccgctga cgagccggat gaactgctat ccggctaacc cgccttccgc gcaatgtcgc
     3301 cgccagtgga ttgctgccgt gcgccgccag cgactgggcc accagattac cccgcgggcg
     3361 acgaaaacct gcccataccg cctgactgag ttcttgagat aatccgccct catccaccag
     3421 ccacaacgcc ccggcaatct cgttttcgcc agccgcctgt aaaaaatgtt gccctggtgc
     3481 atccatcatc cggcgtaaat ccagcggcga ggtccggtag tgcgcaccag acaataactg
     3541 ataaaccttt aacggcgttt ctggctcgct tcgccataac gtttgttcaa atgcggaaat
     3601 gacgatattg ccttgtggtg tatgggtgaa gttttcatcg ttaaaaacca gtgcctcact
     3661 aaccattttt tccagcgggc atccctgtgc ccagcggatc ggctgttgca gttcaaaacg
     3721 gtgtaaatgc ggaaagcgag cgcaaaattt cagcaaaaaa ccacgtccgg taccttcgta
     3781 gccctgcacc gtagtggtta acaacgttcg aggaaaacgc gataccagtt gatgcaacaa
     3841 cggcgcaggt atggctgcgg cttcgtcgac caccagccag tcggcttgct catcgctggc
     3901 taacaaggca tccggtgcaa taaagcgaaa cttctcgccc gcaaattgtg ccagtacatc
     3961 cgttgccgct tttgcgggcg cggtgacaat cgcactgccc gcaatacgag aaatgagttg
     4021 ccctgccaac gccgacttac cgcgcccacg cgcagccgtt accgctgcca cgcccggcgg
     4081 catggtcagt agctgctgta agagttgctg ttgttctggt tgtggtgcgc cggtcgcggg
     4141 gtgccagtca gtacggggag taaaatgcgc caacgagaac ggctggtttt gccgccagag
     4201 gatagcgtcg ttatccgccg taagtacgcg tttgagatgc tggacaaaat gcggcgtcgc
     4261 aatagggtca gggcaatcac tccagcgcag cgagtcggca tcaggttggt tttcccactc
     4321 ttcccataca gggagtaaca aaaccagcca gcttcccgct ttcaacgttc cgctaagtgc
     4381 tgcaaaggca gcggcatcaa agccgtggcg ggcgtcgaat accgcatgcc ggaactcgcg
     4441 cccaagtaaa gtttgtagcg ccgagggtga acagtggttt tcagcatcta gctgcggcga
     4501 aatccacagc cagtcgccag gtaaggcatc acgcaactta agagcatgat caaaacacca
     4561 accctcttcc ccgctcaaca ccagcaagcg gcagatccct tcacgtttca tttgcgctgt
     4621 taatgtgtaa agcgcagtca gttcagccat ccctgccccg gaaattaaat gcttttacca
     4681 aaagtattgc attgtgccgg atcgccgctg tcgaaaccac gtttaaacca gctgtagcgt
     4741 tgctgagaag tgccatgtgt gaaactgtct ggtactactc gcccctgact ttgctgttgt
     4801 aaacggtcat cgccgatggc ctgcgccgca ttcagcgcct cttccagatc gccagtttcc
     4861 agaacgcctt gctgctgcat actatgcccc cagacaccgg caaaacagtc ggcctggagt
     4921 tccatacgca cagataagcg gtttacttcc gcctgcgtcg cgttttgttg cagttgacga
     4981 actttcggct cgatgcctaa cagtttctgc acatgatgac cgacttcatg ggcgataacg
     5041 tacccctggg caaaatcgcc atccgcgcca agtttgtctt tcatgtcatc atagaaggag
     5101 agatcgatat aaactgtgcc atccgccggg caatagaacg gccccataat ggactggccc
     5161 gccccgcagc cggtgcgcgt cattccacgg tacatgacca gcttcggttg ctgataggtc
     5221 ttacccatct tttcgaactg ttgtccccag gtgtcttccg tggttgcctg aatcaccgag
     5281 gtgaattttg cggcttcgtc ttcattaggg ctaattgacc gcgttgattg ttgttgagaa
     5341 accggctgcc cggtcatcaa cccggttaaa tcaacgccat agtagcctgc aaccagcacg
     5401 actatcagta aaatcagccc gcctttaccg ctcggcagac ggaaaccggg accgcccatt
     5461 gacggaccgc cagagctgtt gcgcctgtct tcaacattgt cactttcacg tcgcccttgc
     5521 caacgcatag atacctcaac aatatattca ttatagaaat gatcgtaggt ggttcagagg
     5581 aagattacca caggaaagcg ggatgaaatg gcggaggaat gagcgttcaa tgcatatatt
     5641 ccaagagcgg ttgtcttgcc gtgccagctg cacggcaaga tgatgaacag aaaaatcagt
     5701 ccagctgtac acccaggcgg cgggcgacgg cttcataggc ttcgatcagg ccaccgaggc
     5761 tctggcggaa acggtctttg tccattttct ccagtgtttc tttgtcccac aggcggctgc
     5821 cgtccgggga gaactcatcg cccagtacca cttcaccttt gtacagacca aactccagct
     5881 tgaagtcgac cagaatcaga ccagcatcat cgaacagttt tttcagcacg tcgttcgctt
     5941 tgtaggtcag ctctttcata cgcgccaggt tctctttgct cacccagcca aaggtttcgc
     6001 agtaagattc gttgaccatc ggatcgtgca tggcatcgtt tttcaggaac agatcgaaca
     6061 gcggcgggtt cagctcaata ccttcttcga ttccaagacg tttcactaga gagccagcag
     6121 cacggttacg cacgacacac tcaaccggca ccatatccag ctttttcacc agacattcgg
     6181 tatcggagag cagacgctcc atttgagtcg ggatacccgc ttcagccagt ttgctcataa
     6241 tgaagtagtt gaacttgttg ttcaccatac ctttgcgatc aaactgctca atgcgcgcgc
     6301 catcccctgc tgacgtatca ttgcggaatt cgagcaccaa caggtccggg ttttccgtgc
     6361 tgtatacggt tttcgcttta ccacgataca actcagcttg cttttgcatc tttatcactc
     6421 ctgggtgtga attaacgttt taaaatcttt tgctgtctgg tgtgccggat gttttgtcgg
     6481 atgcggcgtg aacgccttat ccgaactacg tccgacaaac aaatttcgtg cgaattaccg
     6541 ctaaaatcgc gttttcctgc cgacgcacac gtttgcgtat catatcagaa aaaagggccg
     6601 gatgattcca gccctgtatt tttacttgct aaacgcagcc tggaagacag ctaccagcgc
     6661 gtcgttctga ctctgagtca gagtatgacc tttcggatcg atgaactgta ggctgctgcg
     6721 gttatctaaa tcgccaacct gcagtttata gtcaccggat gccaggcctg gatcgctcgc
     6781 gcccagttcc tgccagtcgc tgtcagacag cggcttataa gttacggcca tgttgccctg
     6841 cgaacgggtg ctgtcggtca ctttcatgcc cactttttcc agcgccgctg gcagacgttg
     6901 ccaaaccaca ttgaacggcc cgcgtacgac cagcattggt aaaccggtgt catcagctgc
     6961 actttgtacg tccatagtgg tggaggcacg attttgcgca gcgttcgcgg cgtcagtggc
     7021 agatttatcc agaccggcgg aaataacgtt catcatctcc gtgctgtaac gctgcatgga
     7081 agccgcgtct gcaaccggtt tgcccgcctg ttccaggttc agcagtttaa ccgtaaccgc
     7141 ctgctgataa ccctgcggct taacagagat ttgataacga ccacgatact gctcgtcttc
     7201 gtccagacgg ttccattgta cccaatcggt ggtcagtgtc tgaccagcat catcacgttg
     7261 ggtgatggtg tagtttttcg cctgcagcac gctaaccacc tgcggccaca gagtattgcc
     7321 acgaccattt tccaccagca atgaagcggt atcgcccgtg aactgggtac gcgcgccaga
     7381 aaccagtgcc aacggctggg ctggtggacg aatgtccagc gccttaccga cagcaccact
     7441 accgttggtc accgggattg cataatcacc ggaggtcacc ggcaaaatca ttccagccgg
     7501 ggcatgaagc tccgcaagcg gtgccgcttc caggtaggct tcatcaccac tgacctgacg
     7561 cttatagcgt gagtcagaac tacaggcagc gagtaataaa acaagcgaaa cacccgcaac
     7621 ctttgccagg cgcgactttt gaacagagta agccatcaaa tctccctaaa ctttacagca
     7681 aaccggcatg cttaagcgcc gctctgaccg tctcacgacc actgtcggtg attggtgtca
     7741 ttggcaggcg cagcgtatcg gtcgccacaa gacccagttc cttacatgcc catttcaccg
     7801 ggattggatt gggttcgaca aatagtttgt tgtgtaatgg catcagacgc tgattaataa
     7861 cgcgtgcctc ggcaaaatgc ccttctgctg ccagtttgca catctgggcc atatcacgcg
     7921 ctgcgacgtt agccgtaacg gaaataactc catgaccgcc caattgcatg aagtccagcg
     7981 cgctcgcatc atcgccgctc agcagaacaa aatcatctga aaccagctct ttgatctggt
     8041 ttacacgcgt taagttccct gttgcctctt tgattccgat aatatttttt actttcgcca
     8101 gacggcccac cgtttccggg agcagatcgc agccagtacg ggacggcaca ttatacagaa
     8161 tttgcggcag gtcagtatgc tcagcgatgg ctttgaaatg ctgatacaaa ccttcttgcg
     8221 acggacgatt gtagtaaggg gttaccgtca ggcagccgac gataccactg tcattgaagc
     8281 gctgcgtcag gctaatggct tccgcagtag cgttagcgcc ggtcccggca attaccggaa
     8341 tgcgcccatc agccagatcc agcgtcatca tcaccacatc agcatgttcg tcatgattta
     8401 aggtagcgga ctcgccagtg gtgccaacag aaacgatcgc cgaagtaccg ctggcgacat
     8461 gataatcaat cagttttttc aagctagccc gacagacatt acctttttca tccatcggag
     8521 taacaatcgc gacaatactt cccgtgaaca tgggccatcc tctgtgcaaa caagtgtctc
     8581 aatggtacgt ttggtatggc attaaaagca agcagacaga accgttctga ttgttgtatg
     8641 catgattttt ttatgctttc cttaagaaca actcacccct taaaggaata accagtttga
     8701 cactgtcatc gcaacattat ctggtgatca ctgcgttggg tgccgatcgc cctggaattg
     8761 tgaacaccat cacccgtcat gtcagtagtt gcggctgtaa tattgaagac agtcgcctgg
     8821 cgatgctggg agaagagttc acgtttatca tgctgctttc cggttcatgg aatgccatca
     8881 ctctgattga atcaacgtta ccgttgaaag gtgccgaact ggatctttta atcgtgatga
     8941 agcgcacgac ggcgcgtccg cgtccgccaa tgccagcatc tgtctgggtt caggtcgatg
     9001 ttgcagactc cccgcattta attgaacgct tcacagcact tttcgacgcg catcatatga
     9061 acattgcgga gctggtgtcg cgcacacaac ctgctgaaaa tgaacgggct gcgcagttgc
     9121 atattcagat aaccgcccac agccccgcat ctgcggacgc agcaaatatt gagcaagcgt
     9181 tcaaagccct atgtacagaa ctcaatgcac aaggcagtat taacgtcgtc aattattccc
     9241 aacatgatga acaggatgga gttaagtaat gaatccactg aaagccggtg atatcgcacc
     9301 gaaatttagc ttgccggatc aagacggaga acaagttaat ttgaccgact tccagggaca
     9361 gcgtgttctg gtttatttct acccgaaagc catgaccccc ggctgtaccg tacaggcctg
     9421 cggcttacgc gataacatgg atgagttgaa aaaagcgggc gttgatgtgc tgggtatcag
     9481 caccgataaa cccgaaaaac tctcccgttt tgcggaaaaa gagctgctta actttacgct
     9541 cctgtctgat gaggaccacc aggtgtgcga acaattcggc gtctggggtg aaaagtcctt
     9601 catgggcaaa acctacgatg gtattcatcg catcagcttc ctgattgacg ctgatggcaa
     9661 aatcgaacat gtctttgacg atttcaaaac cagcaatcac cacgacgttg tgctgaactg
     9721 gctgaaagaa cacgcctgat tactttgctc cattccgtgc tggctgcgct tgcggccagc
     9781 atacctcact tctcgtgatc aagatcacat tctcgctttc ccctgcgaca cgggtgtcga
     9841 atccattttt tgctgaacgt taatgaccat catttttgta ccgttcagaa tccagttaat
     9901 acataactta ttgaatagat tgagttaatc agaatggcat cctttataca atatgaaatg
     9961 caatgtttca tatcattttc aaggagccga catgaaccgc tttgtggtgg ccgaaccact
    10021 gtggtgtaca ggatgtaata cctgtctcgc tgcctgttcg gacgtgcata aaacgcaagg
    10081 tttacagcaa cacccgcgcc tggccctggc gaagacgtca acaatcactg cccctgtcgt
    10141 gtgtcatcac tgtgaggaag ccccttgcct gcaggtctgc ccggtcaatg ccatctctca
    10201 gagggatgat gcgatccaac tcaacgaaag cctctgtatt ggctgcaagc tttgcgccgt
    10261 ggtctgccca tttggcgcaa tcagcgcttc aggaagccgt ccggtgaatg cccatgcgca
    10321 atatgttttt caggctgaag gctcactcaa agacggcgaa gaaaacgtgc cgccccaaca
    10381 tgcgttgctg cgctgggaac cgggtgtcca gaccgtcgcg gtgaaatgcg acctgtgtga
    10441 tttcttgcca gaaggtccgg cctgcgttcg cgcttgcccg aatcaggcgt tacggctgat
    10501 caccgacgat agcctgcaac gtcagatgaa agaaaaacag cgccttgccg caagctggtt
    10561 tgccaatggc ggggagtctc ccctttccct cactcaggag caacactaat ggatgccctg
    10621 caattattaa cctggtcgct gatcctctat ctgtttgcca gtctggcttc gctgttttta
    10681 ctcggtctgg acagactggc tattaagctt tccggcatca catcgctggt gggcggcgtg
    10741 attggcatca tcagcggaat tacgcaatta catgctggtg taactttagt cgcccatttt
    10801 gccacccctt ttgactttgc cgatttaacc ctgcgaatgg atagcctctc ggcatttatg
    10861 gtgctagtta tctccttgct ggtggtggtt tgttcgctct attcattgac ttatatgcgc
    10921 gaatacgagg gcaaaggcgc ggcggcgatg ggcttcttta tgaatctttt catcgcatcg
    10981 atggttgccc tgctggtgat ggacaacgct ttttggttca tcgtgctgtt tgaaatgatg
    11041 tcgctgtctt cctggtttct ggtcattgcc aggcaggata aaacgtcgat caacgctggc
    11101 atgctctact tttttatcgc ccacgccgga tcggtgctga ttatgatcgc cttcttgctg
    11161 atggggcgcg aaagcggcag cctcgatttt gccagtttcg cacgctttca ctttctccgg
    11221 ggctggcgtc ggcggtgttt ctgctggcct ttttcggttt tggcgcgaaa gccgggatga
    11281 tgccgttgca cagctggttg ccgcgcgctc accctgccgc accatcgcac gcttcggcgt
    11341 tgatgtctgg cgtaatggtc aaaatcggta ttttcggcat cctgaaagta gcgatggatc
    11401 tgctggcgca aacgggtttg cctctgtggt ggggcattct ggtgatggcg atcggcgcaa
    11461 tctccgcgct cctgggcgtg ctatatgcgc tggcggaaca ggatatcaaa cggctgctgg
    11521 cctggagtac cgtcgaaaac gtcggcatta ttttgctggc agtcggtgtg gcgatggacg
    11581 gtctgtcact gcacgacccg ctgctcaccg tggttggact gctcggcgca ctgtttcatc
    11641 tgctcaacca tgcgctgttc aaagggctgc tatttctcgg cgcggacgcg attatttcgc
    11701 gtttgcatac ccacgacatg gaaaaaatgg gggcactggc gaaacggatg ccgtggacag
    11761 ccgcagcatg cctgattggt tgcctcgcga tatcagccat tcctccgctg aatggtttta
    11821 tcagcgaatg gtacacctgg cagtcgctgt tctcactaag tcgtgtggaa gccgtagcgc
    11881 tacaacttgc gggtcctatt gctatggtaa tgctggcagt cactggtggg ctggcagtaa
    11941 tgtgcttcgt caaaatgtac ggtattactt tctgtggtgc gccgcgcagt acacacgctg
    12001 aagaggcaca ggaagtgcca aatacgatga tcgtcgccat gctactgctc gcggcactct
    12061 gcgtattaat tgcgcttagt gccagttggc tggcaccgaa gataatgcat attgcccatg
    12121 cgtttaccaa tacccctccc gtcactgtcg ccagcggaat agcacttgta cccggcacgt
    12181 ttcatacacg ggtcactcct tcattactgt tgctgttact actggcgatg cctttgctgc
    12241 caggccttta ctggctgtgg tgtcgttcgc gccgcgcagc gtttcgtcgc acaggagatg
    12301 cctgggcatg cggctacggc tgggaaaacg cgatggctcc gtcaggcaat ggcgtgatgc
    12361 agccgctgcg tgtggtcttc tctgcgttat ttcgtctacg acaacagctc gaccctacgc
    12421 tgaggctaaa taaaggtctt gcgcacgtca ccgccagggc tcagagcaca gaacccttct
    12481 gggatgagcg ggtgatccgc cccatcgtga gcgccaccca acggctggtc aaagaaatac
    12541 agcatctgca aagcggcgac tttcgtctct attgcctgta tgtggtcgcc gcactggttg
    12601 tgctgctaat cgctattgcc gtctaaggaa atcaccatga gacaaactct ttgcgacgga
    12661 tatctggtca tttttgcgtt agcacaggcc gtgattctgc tgatgctaac cccacttttt
    12721 acgggtattt cccggcagat acgcgcgcgt atgcactccc gccgcgggcc ggggatctgg
    12781 caggattatc gcgatatcca caaactgttt aaacgccagg aagttgcgcc gacatcttca
    12841 ggtctgatgt tccgcctgat gccgtgggta ttaatcagca gcatgctggt gctggcgatg
    12901 gccttaccac tatttattac cgtttcccct tttgcgggcg gcggcgatct gatcaccctt
    12961 atctatcttc ttgccctgtt tcgttttttc tttgctcttt ccgggctgga taccggaagt
    13021 ccgtttgcgg gagtcggtgc cagtcgcgag ttgacgctcg gcattctggt cgaaccaatg
    13081 cttattctct cactgctggt attggcgctg atagcaggtt ccacgcatat cgagatgatc
    13141 agcaatacgc tggctatggg ctggaactcg ccgctaacca ccgtactggc gttactggcc
    13201 tgtggttttg cctgcttcat tgagatggga aaaattccct ttgatgttgc tgaagcagaa
    13261 caggaattac aggaaggccc gctgaccgaa tattccggtg ccgggctggc gctagcgaaa
    13321 tgggggctgg ggctgaaaca ggtcgtgatg gcagcactgt ttgtggccct gtttctgccc
    13381 tttgggcgcg cgcaagaact ttctctcgcc tgcctgctga cttcacttgt cgttacgctg
    13441 ctcaaggttt tgctgatttt tgtactggcc tcaatcgcag aaaacacgct ggcacgcggg
    13501 cgttttttac tcattcacca tgtgacctgg cttggcttca gccttgctgc gcttgcatgg
    13561 gtcttttggt taaccggtct gtaaggagca ctgacggaat atggaaaatc ttgctctgac
    13621 gacgttaatg ctgcctttta tcggcgcact ggtcgtttcg ttttcgccac aacgtcgggc
    13681 cgccgaatgg ggggttttgt tcgccgcgct gaccacgctg tgcatgttgt cgctgatctc
    13741 cgcgttttat caggccgata aagttgccgt cacgttgacg ttggtcaacg tgggggatgt
    13801 ggcattattt ggcctggtca ttgatcgcgt gagtacgctg attctgtttg tggtggtgtt
    13861 tctcggtttg ctggtcacga tctactccac gggttatctg acggataaaa atcgcgaaca
    13921 cccgcataac ggcacgaatc gttattacgc atttttgctg gtgtttatcg gcgcgatggc
    13981 gggactggta ctctcctcga cgctgctcgg tcagttgttg ttttttgaaa ttacgggcgg
    14041 ctgctcctgg gcgttgatca gttattacca gagcgataaa gcgcagcgtt cagcactaaa
    14101 agcgttgctt atcacccata tcggttcgtt ggggttgtat cttgccgccg ccacgctgtt
    14161 tttgcagacc ggaacgtttg tgcttagcgc gatgagcgag ttacatggcg acgcacgtta
    14221 tctggtttat ggcggcatcc tgtttgccgc gtgggggaaa tcggcccagc taccgatgca
    14281 agcgtggcta ccggacgcaa tggaagcgcc aacaccgatc agcgcctatc tccacgccgc
    14341 atcgatggtg aaagtgggcg tttacatttt tgcccgcgcc attatcgacg gcggcaatat
    14401 cccgcatgtg attggcggcg ttggcatggt catgacactg gtcaccattc tttatggctt
    14461 tctgatgtat ttgccacagc aggatatgaa gcggttgctg gcctggtcga ccatcactca
    14521 acttggctgg atgttcttcg gcttgtcgct ctccatcttc ggctcgcggc tggcgctgga
    14581 gggtagcatc gcctacatcg tcaaccacgc gttcgctaaa agcctgtttt tccttgtagc
    14641 aggtgcgctg agttacagct gcggcacgcg cttgttgccg cgtctgcgtg gcgtattgca
    14701 caccctgccg ttgccaggcg tgggtttctg cgtggcagcg ctggcaatta ccggtgtgcc
    14761 gccgttcaac ggcttcttca gtaaattccc gctgtttgcc gccggttttg cgttgtcagt
    14821 ggagtactgg atcctgctgc ccgccatgat tctgctgatg attgaatcgg tcgccagttt
    14881 cgcctggttt attcgctggt ttggtcgcgt cgtgccgggc aaaccgagcg aggccgtcgc
    14941 cgatgccgca ccgctgccag ggtcaatgcg cctggtgttg attgtactga ttgtgatgtc
    15001 gctgatttcc agcgtaatcg ccgcgacctg gttgcagtaa ggagatgatg aatgaccggt
    15061 tctatgatcg taaataatct ggcgggactg atgatgctga catcgctgtt tgtgattagc
    15121 gtcaaaagct atcgcctgtc atgcggattt tacgcctgcc agtcgctggt gctggtgtct
    15181 attttcgcca ctctctcgtg cctgttcgcc acagagcaac tgctgatctg gtccgccagc
    15241 gcctttatca ccaaagtgct gctggtaccg ttaatcatga cttacgctgc acgaaatatt
    15301 ccccagaaca tcccggaaaa agcgttattc ggtccggcaa tgatggcact gctcgcggcg
    15361 ttaattgtcc tgctttgcgc atttgtcgtt cagcccgtga agctaccgat ggctaccggg
    15421 ctgaaaccgg cgctggcggt agcgttaggt cattttctgc ttggcctgct gtgcattgtc
    15481 agccaacgca atatcctgcg gcaaattttt ggttactgcc tgatggaaaa cggctcccat
    15541 ctggtgctgg cgcttcttgc ctggcgagca ccggaactgg tggaaatagg tatcgctacc
    15601 gacgccatct tcgccgtcat tgtgatggtg ttactggcaa gaaaaatatg gcgtacccac
    15661 ggcacgctgg acgtgaacaa cttgaccgcg ctgaagggat aatgagatga gttattctgt
    15721 gatgttcgct ttactcctgc tcacgccgct gcttttttcg ctgctctgtt ttgcctgccg
    15781 gaaacggggg ctctctgcga cttgtacggt gactgtatta catagcttag ggatcacact
    15841 gctgctgatt ctagcactct gggtggtcca aactgccgct gatgcaggag aaatattcgc
    15901 tgcgggactg tggcttcata ttgatggtct gggcggtttg ttcctcgcca ttcttggtgt
    15961 gattggcttt ctcaccggtg tttactcgat tggctacatg cgtcatgaag tggcacacgg
    16021 cgagctttca cccgttacac tgtgcgatta ctacggtttc ttccatctgt ttttgttcac
    16081 catgctgctg gttgttacca gcaataacct gattgtgatg tgggcggcga tcgaagccac
    16141 caccttaagc tcggcgtttc tggtaggcat ttacggtcag cgttcatcgc tggaatcagc
    16201 atggaagtac atcattattt gtactgttgg tgtcgctttt ggtctgttcg gtaccgtgct
    16261 ggtatacgcc aacgccgcca gcgttatgcc gcaggcagaa atggcgatat tctggagcga
    16321 ggttcttaag caatcgtcct tgcttgaccc aacattaatg ctgttggcct ttgtgtttgt
    16381 gctaattggc tttggtacca aaaccgggct atttcccatg cacgcctggc tgccggatgc
    16441 tcacagtgaa gcgccgagtc cggtcagcgc cctgctctcc gccgtattgc tgaactgcgc
    16501 gctgttggtg ctgattcgct attacatcat tatttgccag gccatcggca gcgatttccc
    16561 caaccggttg ttgctcatct tcggcatgtt gtcggttgcc gtggcggcat ttttcattct
    16621 ggtacagcgg gacattaagc gtctgctggc gtactccagc gtggagaaca tggggctggt
    16681 cgcggtggcg ctaggcattg gcgggccgct gggaattttt gccgcgctgc tgcacacctt
    16741 aaaccacagt ctggcaaaaa cgctgctgtt ctgcggttcc ggcaatgtac tgctcaagta
    16801 cggcacgcgc gatctcaacg tcgtctgtgg gatgctcaaa atcatgccat ttaccgccgt
    16861 gctgtttggc ggcggtgcgc tggcgctggc gctggcgctg gcagggatgc cgcccttcaa
    16921 catttttctt agcgaattta tgaccgttac cgcaggactg gcacgtaatc acctgctgat
    16981 tatcgtcctg ctgttattgc tgttaacgct ggtgctggcg ggcctggtac ggatggctgc
    17041 gcgggtgtta atggcgaaac cgccgcaggc cgttaaccgg ggtgatctcg gctggttgac
    17101 cacctcgcca atggtgattc tgctggtcat gatgctggcg atgggaacgc atattccaca
    17161 acctgtcatc aggatcctgg cgggcgcttc cactatagtc ctctcaggga cgcacgacct
    17221 gcctgcacaa cgtagcacct ggcatgattt tttgccttca ggcaccgcat ctgtttcgga
    17281 gaaacacagt gaacgttaat tcatcgtcaa atcgtggcga agcgattctc gccgccctga
    17341 aaacgcagtt ccccggcgcg gtgctggatg aagagcgaca aacgcctgaa caggtcacca
    17401 ttacggtgaa aatcaatctg ctgcctgacg ttgtacatta tctttattat caacatgatg
    17461 gctggcttcc agtcctgttt ggcaacgacg agcggacact taacggtcat tacgcggttt
    17521 attatgccct ttcaatggaa ggggccgaaa aatgctggat cgtggtgaag gcactggtcg
    17581 atgccgacag tcgggagttt ccgtcagtca caccgcgcgt ccctgccgcg gtctggggcg
    17641 agcgagaaat tcgcgatatg tacgggctga ttccggttgg cctgccggat cagcgtcgcc
    17701 tggtgttgcc cgatgactgg ccggaagata tgcatccgct gcgcaaagat gcgatggatt
    17761 atcgactgcg ccctgaaccg acgacagatt ccgaaacgta tgcgtttatc aatgagggca
    17821 acagcgatgc gcgggtgatc cctgtcggcc cgctgcatat cacctcctat gaaccgggtc
    17881 acttccgctt gtttgtggat ggcgagcaaa ttgtcgatgc tgattaccgc ctgttttatg
    17941 tccatcgcgg catggagaaa ctggcagaaa cgcggatggg ctacaacgaa gtgaccttct
    18001 tatcggaccg cgtgtgtggg atttgcggtt ttgcccacag tgtggcctat accaactcgg
    18061 ttgaaaatgc actggggatt gaggtgccgc aacgagcaca tactattcgc tcgattctgc
    18121 tggaagtcga gcggctacac agtcatttgc tcaaccttgg cctctcctgc cattttgttg
    18181 gttttgatac cggctttatg caatttttcc gcgtgcggga aaagtcgatg acgatggcgg
    18241 aattgctgac cgggtcgcgt aaaacctacg gtctgaatct gattggtggt gttcgccgcg
    18301 atattctcaa agagcaacgt ctgcaaacgc tgaaactggt gcgcgagatg cgcgccgacg
    18361 tgtcggagct ggtagagatg ctgcttgcta cgccgaatat ggaacaacgc actcagggca
    18421 ttggcattct cgaccgacaa atcgcccgtg attatagccc tgtagggccg ctgatccgcg
    18481 cagtggtttt gcccgtgatt tgcgctttga tcacccctac gccgactacg gcaatattcc
    18541 aaaaacactg tttaccttta ccggcggcga tgttttctcc cgcgtgatgg tccgtgtcaa
    18601 agagacgttt gattcgctgg caatgctgga atttgccctc gacaacatgc cggatacccc
    18661 actgctgacc gaaggcttta gctataaacc tcacgcattc gcgctgggct ttgttgaagc
    18721 gccacgcggt gaagacgtgc actggagcat gctcggtgat aaccaaaaat tgttccgctg
    18781 gcgctgtcgt gccgccacct acgccaactg gccggtgttg cgttacatgc tgcgcggcaa
    18841 taccgtttct gacgcaccgc tgattatcgg tagccttgat ccctgctact cctgtaccga
    18901 ccgtgtgacg ctggtagatg tgcgcaagcg ccagtcaaaa accgtgctgt ataaagagat
    18961 cgaacgctac ggcattgatc gtaaccgttc gccgctgaag taaggacaga agatgctgaa
    19021 gttactgaaa actattatgc gcgccggaac cgcgacggtg aaatatccct tcgcgccact
    19081 ggaggtcagc ccaggctttc gcgggaaacc ggacctgatg cccagccaat gtattgcctg
    19141 cggtgcctgc gcctgtgctt gtccggcaaa tgcgctgact atccagaccg acgaccagca
    19201 aaattcgcgc acctggcagc tctatctggg gcgttgtatt tactgcggac gttgtgaaga
    19261 agtgtgcccg accagagcca tccagcttac caataacttt gaactgaccg tcacccataa
    19321 agccgatctc tatacccacg cgacgttcca tctacaacgt tgcagccgtt gcgaacgccc
    19381 gtttgccccg caaaaaaccg tcgcactggc tgctgaattg ttggcacagc aacaaaatgc
    19441 tccacaaaac cgcgaaatgt tatgggcgca agcgagcgtc tgcccggagt gcaaacaacg
    19501 cgcaacgctg atcaacgacg atacagatgt accgctggtg gctaaggagc agctatgagt
    19561 ccagtgctta cgcaatatgt cagccagccc atcacgctgg acgagcaaac gcaaaagatg
    19621 aagcggcatt tgctacagga tatccgtcgc tcggcttacg tttatcgcat cgattgcggc
    19681 ggctgcaacg cctgtgaaat cgaaattttt gctgccatca cccccgtatt cgacgcagaa
    19741 cgttttggca ttaaggttgt ttcatcaccg cgtcacgccg atattttgtt atttactggc
    19801 gcagtcaccc gggcgatgcg tatgcctgca cttcgggcgt atgagtctgc ccccgatcat
    19861 aaaatctgtg tttcctacgg cgcgtgcggt gtcggcggcg gtattttcca cgatctctac
    19921 agcgtctggg gcggcagcga caccattgtc cccattgatg tttggatccc cggctgcccg
    19981 ccaacaccgg ccgccaccat tcacggtttc gccgtggcgc tcggtttgct gcaacagaag
    20041 attcacgcgg tggattatcg cgatcccacc ggggtgacta tgcaaccgtt gtggccgcag
    20101 atcccgccat cacagcgtat cgccattgag cgagaagcgc ggcggctggc gggctatcgt
    20161 caggggcgag aaatttgcga tcggctcctg cgccatttaa gcgacgatcc tacaggaaat
    20221 cgggttaaca cctggttgcg cgatgccgac gatccacgtc tcaatagtat cgttcagcaa
    20281 ctctttcgcg tactccgggg gttacatgac tgaagagtgc ggggaaattg ttttctggac
    20341 gctgcgaaaa aagtttgtcg ccagtagcga cgagatgccg gaacacagct ctcaggtaat
    20401 gtattactcg ctggctatcg gtcatcacgt tggcgtgatt gattgtctga atgtcgcctt
    20461 ccgctgccca ctgacggaat acgaagattg gcttgcactg gtcgaagagg agcaagcccg
    20521 acgtaagatg ctgggggtga tgacttttgg tgagattgtt attgacgcca gccacaccgc
    20581 cctgttgacc cgggcattcg cgccactggc agatgacgcg acgtctgtgt ggcaagcgcg
    20641 tagcattcaa ttcattcatc tgttggatga aattgtgctg gaaccggcta tctatctgat
    20701 ggccagaaaa attgcgtgag aaggatttct cattaataag gactgttgat ggctatgtca
    20761 gacgaagcga tgtttgcccc gccgcaagga ataacaattg aagcggtaaa cggaatgctc
    20821 gcggagcggt tagcgcagaa acacggcaag gcgtctttat tacgcgcctt catcccgctg
    20881 ccgccgccgt tcagcccggt acaacttatt gaactgcatg ttctcaaaag caacttctat
    20941 taccgctacc atgatgatgg cagcgatgtg acggcaacaa cagagtatca gggcgagatg
    21001 gtcgattatt cgcgttacgc cgtccttctc ggcagtagtg gaatggcgga gctacgcttt
    21061 attcgtaccc acggcagccg ttttactccc caggattgca cactgtttaa ctggctggca
    21121 cgcattatca ccccggttct gcaatcatgg ctcaatgatg aagcacagca ggtggcgctg
    21181 cgtttgctgg agaaagatcg cgatcatcat cgggtactgg tggatatcac taatgcagtg
    21241 ctgtcacatc ttgatctcga cgatctgatc gctgacgtcg ctcgtgagat ccatcatttt
    21301 ttcggtctgg cttcagtcag tatggtactg ggcgatcatc gaaagaacga gaagttcagc
    21361 ctgtggtgca gcgatctttc tgcctcacat tgtgcgtgtc tgccacacaa tatgcctggc
    21421 gacagtgtat tgctgacaca aacgctacaa acccgacaac cgaccttgac gcaccgtgca
    21481 gatgatctgt ttctctggca acgcgacccg ttattactct tacttgcatc taacggctgc
    21541 gaatctgcgc tccttatacc gcttaccttt ggcaaccata caccgggtgc attgttgctg
    21601 gcgcatacct cttccactct ctttagtgag gaaaactgcc agctactaca acacatagcc
    21661 gatcgcatcg ctattgccgt tggcaatgcc gatgcctgcc gtagaatgac cgatttgcag
    21721 gaaagtttgc agcaagaaaa ccaccagctt agcgagcagc tcctttcgaa tctgggcgtc
    21781 ggtgacatta tctatcaaag ccaggcaatg gaagacctgc tccagcaggt agatattgtg
    21841 gcgaagagcg acagtacggt gttgatttgc ggtgaaaccg gaaccggcaa agaggtgatc
    21901 gccagagcga tccatcaact tagcccgcga cacgacaagc cgctggtcaa aatcaactgc
    21961 gctgccatcc ccgccagtct tctggaaagt gagttattcg gtcatgacaa aggggcgttt
    22021 actggtgcga ttaataccca tcgtggtcgt tttgaaattg ccgatggcgg cacgttgttt
    22081 ctcgatgaaa ttggcgatct gccgttagaa cttcagccta aactgctgcg cgtattgcag
    22141 gaacgggaga ttgagcgtct cggcgggagt agaacgatcc cggtaaatgt cagagtcatt
    22201 gccgccacca accgtgattt gtggcaaatg gttgaagatc gccagtttcg cagcgatctc
    22261 ttttatcgcc tgaatgtctt cccactggaa ttgcctccgc tgcgcgaccg tccggaagat
    22321 atccctcttt tagcaaagca tttcacgcaa aaaatggcgc gccatatgaa tcgttcaatt
    22381 gacgccatcc cgaccgaggc gctacgccag ttgatgtcgt gggactggcc gggcaacgtg
    22441 cgcgagctgg aaaacgtgat tgagcgagcg gtactgctga ctcgcggtaa cagtctgaat
    22501 ttacatctta atgtccgaca aagccgttta ctgccgacgc taaatgaaga ttcagcgctt
    22561 cgcagttcaa tggcgtagtt gctgcacccg acgacgccag agaatgacga agaagaacgt
    22621 cagcgcattg ttcaggtatt gcgagaaacc aatggcattg ttgccgggcc ccgtggcgcg
    22681 gcgacacgat tagggatgaa gcgcaccacg ctgctgtcac gaatgcagcg tctgggaatc
    22741 tcggttcgcg aggtgttgta atctgctttt gcaggagtat gcatgagaaa caaactctct
    22801 ttcgacttgc agttgagcgc cagaaaagcg gcaatcgctg aacggattgc cgcccataaa
    22861 attgcccgca gtaaagtgtc ggtcttttta atggcgatgt ccgctggcgt gtttatggcg
    22921 atgtccgctg gcgtgtttat ggcgatcgga tttacttttt acctttccgt tatcgccgat
    22981 gccccgtctt cataggcatt aacccatctg gtgggcggcc tttgctttac acttggcttt
    23041 attttgctgg cggtttgcgg caccagcctg ttcacctcgt cggtaatgac ggtaatggca
    23101 aaaagtcggg gcgttattag ttggcgaact tggctgatta acgcacttct ggtggcctgc
    23161 ggtaatctgg caggtattgc ctgtttcagt ttgttaatct ggttttccgg gctggtgatg
    23221 agtgaaaacg cgatgtgggg agtcgcgatt ttacactgcg ccgagggcaa aatgcatcat
    23281 acatttactg aatctgtcag cctcggcatt atgtgcaatc tgatggtttg cctggcgctg
    23341 tggatgagtt attgcggacg ttcgttatgc gacaaaatcg tcgccatgat tttgtccatc
    23401 accctgtttg tcgccagtgg ctttgagcac tgtatcgcca atttgtttgt gattccgttc
    23461 gccattgcca ttcgccattt cgcccctacc tccttctggc aactggcgca cagtagcgca
    23521 gacaattttc cggcactgac ggtcagccat tttattaccg ccaatctgct cccggtgatg
    23581 ctgggtaata ttatcggcgg tgcagtgctg gtgagtattt gttatcgggc tatttattta
    23641 cgtcaggaat cctgaaaaat cagcccggcg aaacagttcg tcgggctatt tatttacgtc
    23701 aggaatcctg aaaaatcagc ccggcgaaac agttcgtcgg gctgaagatt attcttgcgc
    23761 gatttgccca tcgggccagg cgtggatcac ggctttgatc agcgtcgcca atggaatggc
    23821 gaagaatacg ccccagaatc cccacaaacc accgaagatc accaccgata aaataatcac
    23881 cagcggatgc aggttaaccg cttcggagaa caacaccggt actagcaggt tgccgtccag
    23941 cgcctgaata atcagataca ccgcgaagca gctccagaat tcagtacctg cgccaaactg
    24001 gaatagcgcc acgccaacca ccggaatggt caccacaaat gcgccaatgt acggaataag
    24061 aaccgagaag ccgaccagca ctgccagcag cagcgaatag ttcagcccga agagcaagaa
    24121 ccccagccag gtggcgatcc ccaccacgat catctccagc actttgccgc ggatatagtt
    24181 ggtgatttgt tgattcatct ccttccacac ctgtcctgcc agcccacggt tacgcggcag
    24241 cacccggcga acggcgttca gcatctgctc tttgtctttc agcaggaaga agaccatcaa
    24301 tggcaccagc accagataga cggctatggt cagcaaaccg accagcgagg cgagggaaat
    24361 tttcaccacc gaatcgccca tggtcagcat ccgactgcgc atattttcgg ccattgcatc
    24421 aataatgccc gcgtccatta acaccggata gcggcgcggc aacgtggcgg caaagtcaga
    24481 aagcttattg agcatccccg gcatatcgcg gattaagtag atgccctgtt gccaggcgat
    24541 aggcagtacc acgaacgcca tcagtagcaa tataccgaca aaaaccacca atacaatcga
    24601 cgtcgcccag cggcgggagc agccaataga ttgcaggcgc acggttggcc attccagcaa
    24661 ataagccagc acaatagcca ccagcaacgg agcaagcagg ccactaaaga aaaagataat
    24721 gccaaatccg gcaactaaaa taaccagcaa ggcaatcgct tccgggtcgc taaaacggcg
    24781 gcgataccat tgcatcaaca tttcgagcat aaagaccttc cctgaacctc aagagcggga
    24841 ttgcgatccg caattgtatc gaaatgtcac aaaaaagact tcgcttttta tgacggattc
    24901 aggaaactga aaagtcattt gagtgggcta atcttcgccg ttacactcaa aggcggcgag
    24961 gtgggaacga tatttcacag tatcggtcaa atgactatct tcagaaatac aggatagagg
    25021 ttatgttcag gcagttgaaa aaaaacctgg ttgcaaccct cattgctgct atgaccattg
    25081 gtcaggtagc cccggcgttt gccgacagcg cagacacctt gccggatatg ggaacctccg
    25141 caggaagcac gctttccatt ggtcaggaaa tgcagatggg cgactattat gtccgccagc
    25201 tacgcggcag cgcgccgtta attaatgacc cgctgttaac gcaatatatt aattcgctgg
    25261 ggatgcgtct ggtttcgcat gccaattcgg ttaagacacc gtttcatttt tttctgatca
    25321 acaacgacga aattaacgcc tttgctttct ttggcggcaa cgtggtgctg cactctgccc
    25381 tgttccgtta ttccgataac gaaagtcaac tggcttcagt tatggcgcac gaaatctccc
    25441 acgtcaccca acgtcacctg gcgcgagcga tggaagatca acaacgcagc gcgccgctga
    25501 cctgggtcgg cgcgttaggt tctattttac tggcgatggc cagtccgcag gcggggatgg
    25561 cggcgctgac cggtacactg gcgggaacgc gccaggggat gatcagtttc acccagcaaa
    25621 atgaacagga agcggaccgc attggtattc aggtgctgca acgctcggga ttcgatccac
    25681 aggcgatgcc gaccttcctg gaaaaattgc tcgatcaggc gcgttactcc tcgcgcccgc
    25741 cggaaatttt attgactcac ccgttgccgg aaagtcgtct ggcagatgcc cgcaaccgtg
    25801 ctaatcagat gcgcccgatg gtggtgcagt cgtcggaaga tttctatctg gcgaaagcgc
    25861 gcacactggg gatgtataat tccggacgta accagctcac cagtgatttg ctggatgaat
    25921 gggcgaaagg aaacgttcgt cagcaacgag cggcgcaata tggtcgtgct ttacaggcga
    25981 tggaagccaa taaatacgac gaggcgcgta aaacgctgca accgttactg gcggcagaac
    26041 ctggcaacgc atggtatctc gatctggcta ccgatatcga tcttgggcaa aacaaagcca
    26101 atgaggcaat caatcgcctg aaaaatgccc gcgatttacg caccaatccg gtgttgcagc
    26161 tcaacctggc gaacgcttat ctgcaaggcg gtcaaccaca agaagcggcc aatattctga
    26221 atcgctacac ctttaataat aaagatgaca gcaacggctg ggatttgctg gcacaggcgg
    26281 aagccgcgct aaataaccgc gatcaggagc tggctgcgcg agcagaaggt tatgcgctcg
    26341 ccggacgact cgatcaggcc atttcgctgt tgagtagcgc cagttcgcag gtgaaattag
    26401 gcagcctgca acaagcgcgt tacgatgcgc gcatcgacca gttgcgccag ctgcagaaac
    26461 gctttaagcc ttataccaag atgtaattcg cattaaagga agaacaatga ccaaacaggt
    26521 aaaaatctac cataacccac gctgttcaaa gagccgggaa acgctgaacc tgctgaaaga
    26581 aaacggcgtg gagccagaag tggtgctcta ccttgagaca cccgccgatg cggcaacgct
    26641 gcgcgatttg ctgaaaatgc tggggatgaa tagcgcccgt gaattgatgc gccagaaaga
    26701 ggatctttat aaagaactaa acctggctga cagttcgctt agcgaagagg cgctgattca
    26761 ggcaatggtg gataatccga agctgatgga gcgcccgatt atggtcgcga atggcaaagc
    26821 gcggattggt cgtccaccgg agcaggtact ggaaatcgtg ggttaagatg tgctccatcg
    26881 ctagttgaag cacattgccg gatgcgacgc ttgccacgtc ttatccggcc tacgaatact
    26941 atggattaac ttcgcgtagg tcaggcgttc gcgccgcatc cgacaataaa caccttatct
    27001 acaacttcag aatttctttc acaaacggaa tggtcagctt acgttgcgcg gtaatcgacg
    27061 cacgatccaa ctgatccaac gtcataaata gcgtgcgcat ttctctgtcg agccgcttca
    27121 gcaagaaacg ccccacatct tccggcagtt caaaaccacg caaacgcgcg cgtaactgta
    27181 gcgcctgcaa cttatcttca tcagaaagtg gctgcaattt gtagatctgc ccccagtcga
    27241 gacgcgacgc gagatccggt aatcccagat tcaactgccg cggtggacga tcgccggtga
    27301 tcaacaaccg tgttttgccc gattccagaa ttcgattgta gagatcgaaa atcgccatct
    27361 cccacaactc atcgcctgca atacactcaa tgttgtcgat acagaccagc gacaaatgct
    27421 ccataccgtc gagcacttcc ggaacaaacc aggtgcgttt atccagcggg acatagccca
    27481 ccgcatcgcc acgctgcgac aattccgcgc aagccgcgtg cagcagatgg ctgcgccccg
    27541 cgccttcgcg tgcccagaga tagatgtaac cgctatgttc ctgacgcagc acgttttgca
    27601 gcgcggccag taaagaggag ttatcccccg gccagaaact tgcaaaggtt tcgtcgtcag
    27661 gaagataaag tggcaaagag agctgtgccg gtgtgttcag agatacctca accaggattt
    27721 cacaaaatcg cgagaagttt accacagaat cccatgatgt ttgaaccggg cagcaacact
    27781 gcccggtcgg tacattattt gtctgttata tccgcgtctt ctgcgtccag caccacttct
    27841 tcagggcgca gcacactgat cagtttgaaa atcaaactta agccgatacc gacgatggtc
    27901 gccagcgcca taccttttaa ctccgccgcg ccgatgttta ccttcgcgcc actgacgccg
    27961 atgatcaaaa tcacggaagt caggatcagg ttctgtgctt tgttgtaatc cactttcgat
    28021 tcgatcaaaa cacgaatacc ggaagcacca atgacaccat aaagcagcag cgaaacgccg
    28081 cccatcaccg gcaatgggat catctggata gcggcagcca gtttaccgac gcaggaaagc
    28141 aggatagcga aaatcgccgc cccgccgata acccaggtac tgtaaacacg ggtgatcgcc
    28201 atcacgccaa tgttttctcc gtaagtggta tttggcgtag agccaaagaa gccggagatc
    28261 acggtcgaca ggccgttagc aaacatcgaa cggtgcagac ctggatcgcg cagcagatct
    28321 tttttgacga tattagccgt tactaccagg tgccctacgt gttcggcaat aaccactaac
    28381 gccgctggca gaatagtcag aatggcaaac cactcgaagc gcggcgtata gagggttggc
    28441 agtgcaaacc agtgagcatt aataatcggc gtggtatcga caattcccat tgcgaaagag
    28501 agcgcgtacc ccaccagcac gccaattaaa atcgggataa ttgccaggaa accacgaaac
    28561 agcacggaac ctaaaaccgt gaccgccagg gtggtaatag agatgatgat ggttttggag
    28621 tttggcgttt gcccttcagc cgggagtaaa cccgccatac cggcagctac gcccgccagc
    28681 tccagaccga tgacggcaac gattgcgccc attgccgcag gtggaaacag cacgtccaac
    28741 cagccggtcc ccgctttctt cacgataaaa gaaaccaggc agaacagcac gccgcacata
    28801 ataaagccgc ccagcgcgac ttcataccct aacggcaaca gtaacaatac cggtgaaata
    28861 aaggcaaagc tggaaccaag ataagccgga attttccctt tacagatgaa gagatacagc
    28921 agcgttccaa taccgttaaa taacagtaca gtcgccgggt taatatgaaa taagacgggc
    28981 accaggacgg ttgcaccaaa catggcgaac aaatgttgca aactaagcgg gattgtctgt
    29041 aaaagtggcg gtctttcact caccccgata gcacggcgcg tcatagtatt atcctctgta
    29101 ttatgtgtta taggcgcttt actcaaaaaa aagccgactc ttaaagtcgg ctttaattat
    29161 ttttattctt tatttcgtac caaagatttt gtcaccggca tcgccgaggc ccggaataat
    29221 gtatccgtgc tcgttcagtc cctggtcaat cgatgcagta tacagttcga cgtccgggtg
    29281 cgctttttcc agcgcagcga taccttctgg cgcagcgacc agcaccagaa ctttgatgct
    29341 gctgcagccc gcttttttca gcaggtcgat ggtcgcgata acggaaccac cggttgccag
    29401 cattgggtca acgatcagcg ccatacgctc atcgatgtta gaaaccagtt tctggaagta
    29461 cggtaccggc tccagcgttt cttcattacg gtacataccg acaacgctga tgcgcgcgct
    29521 cggaacgttt tccagcacac cgtccatcat accaagaccc gcacgcagaa ttggcacaac
    29581 ggtaattttc ttacctttga tctggtcgat ttctaccggg ccgttccagc cttcgatagt
    29641 tactttttcc gtttcgaggt cggcggtcgc ttcgtaagtc agcaggctac ccacttcgga
    29701 agcgagttcg cgaaagcgct tggtgctgat atcttgctca cgcatcagtc ccagcttgtg
    29761 tttgacgagt gggtgtttga cttccacgat cttcatactc tttctccttt gaggggcagc
    29821 cacaaaaaaa tcgacggatt atacctcctt tcttcagggc ggcaatatta ttttcgttga
    29881 ctttagtcaa aatgataaca gtttgagata aagttatttt atattcaaat ggttatgaaa
    29941 gaagattatt ccatccgaaa actaaccttt gccctggcac aagtcttctt tcgccgcgcg
    30001 cccggggaaa agacgtgcaa aaaggttgtg taaaacagtc tcgcaaacgt ttgctttccc
    30061 tgttagaatt gcgccgaatt ttatttttct accgcaagta acgcgtgggg acccaagcag
    30121 tgaccgataa aacctctctt agctacaaag atgccggtgt tgatattgac gcgggtaatg
    30181 ctctggttgg aagaatcaaa ggcgtagtga agaaaacgcg tcgtccggaa gtgatgggcg
    30241 gtctgggcgg cttcggtgcg ctgtgtgcat tgccgcaaaa atatcgtgaa cccgtgctgg
    30301 tttccggcac tgacggcgta ggtaccaagc tgcgtctggc aatggactta aaacgtcacg
    30361 acaccattgg tattgatctg gtcgccatgt gcgttaatga cctggtggtg caaggtgcgg
    30421 aaccgctgtt tttcctcgac tattacgcaa ccggaaaact ggatgttgat accgcttcag
    30481 cggtgatcag cggcatcgcg gaaggttgtc tgcaatcggg ctgttctctg gtgggtggcg
    30541 aaacggcaga aatgccgggg atgtatcacg gtgaagatta cgatgtcgcg ggtttctgcg
    30601 tgggcgtggt agaaaaatca gaaatcatcg acggctctaa agtcagcgac ggcgatgtgc
    30661 tgattgcact cggttccagc ggtccgcact cgaacggcta ttcgctggtg cgcaaaattc
    30721 ttgaagtcag cggttgtgat ccgcaaacca ccgaacttga tggtaagcca ttagccgatc
    30781 atctgctggc accgacccgc atttacgtga agtcagtgct ggagttgatt gaaaaggtcg
    30841 atgtgcatgc cattgcgcac ctgaccggcg gcggcttctg ggaaaacatt ccgcgcgtat
    30901 tgccagataa tacccaggca gtgattgatg aatcttcctg gcagtggccg gaagtgttca
    30961 actggctgca aacggcaggt aacgttgagc gccatgaaat gtatcgcacc ttcaactgcg
    31021 gcgtcgggat gattatcgcc ctgcctgctc cggaagtgga caaagccctc gccctgctaa
    31081 atgccaacgg tgaaaacgcg tggaaaatcg gtatcatcaa agcctctgat tccgaacaac
    31141 gcgtggttat cgaataatga atattgtggt gcttatttcc ggcaacggaa gtaatttaca
    31201 ggcaattatt gacgcctgta aaaccaacaa aattaaaggc accgtacggg cagttttcag
    31261 caataaggcc gacgcgttcg gccttgaacg cgcccgccag gcgggtattg caacgcatac
    31321 gctcatcgcc agcgcgtttg acagtcgtga agcctatgac cgggagttga ttcatgaaat
    31381 cgacatgtac gcacccgatg tggtcgtgct ggctggtttt atgcgcattc tcagcccggc
    31441 gtttgtctcc cactatgccg ggcgtttgct gaacattcac ccttctctgc tgccgaaata
    31501 tcccggatta cacacccatc gtcaggcgct ggaaaatggc gatgaagagc acggtacatc
    31561 ggtgcatttc gtcaccgatg aactggacgg tggcccggtt attttacagg cgaaagtccc
    31621 ggtatttgct ggtgatacgg aagatgacat caccgcccgc gtgcaaaccc aggaacacgc
    31681 tatttatcca ctggtgatta gctggtttgc cgatggtcgt ctgaaaatgc acgaaaacgc
    31741 cgcgtggctg gatggtcaac gtctgccgcc gcagggctac gctgccgacg agtaatgccc
    31801 ccgtagttaa agcgccagct ctgccgctgg cgtttttcaa ttcacctgta aatcgcaagc
    31861 tccagcagtt tttttccccc ttttctggca tagttggaca tctgccaata ttgctcgcca
    31921 taatatccag gcagtgtccc gtgaataaaa cggagtaaaa gtggtaatgg gtcaggaaaa
    31981 gctatacatc gaaaaagagc tcagttggtt atcgttcaat gaacgcgtgc ttcaggaagc
    32041 ggcggacaaa tctaacccgc tgattgaaag gatgcgtttc ctggggatct attccaataa
    32101 ccttgatgag ttctataaag tccgcttcgc tgaactgaag cgacgcatca ttattagcga
    32161 agaacaaggc tccaacgctc attcccgcca tttactgggc aaaattcagt cccgggtgct
    32221 gaaagccgat caggaattcg acggcctcta caacgagcta ttgctggaga tggcgcgcaa
    32281 ccagatcttc ctgattaatg aacgccagct ctccgtcaat caacaaaact ggctgcgtca
    32341 ttattttaag cagtatctgc gtcagcacat tacgccgatt ttaatcaatc ctgacactga
    32401 cttagtgcag ttcctgaaag atgattacac ctatctggcg gtggaaatta tccgtagcga
    32461 tagcatccgt tacgcgctgc tggagatccc atcagataaa gtgccgcgct ttgtgaattt
    32521 accgccagaa gcgccgcgtc gacgcaagcc gatgattctt ctggataaca ttctgcgtta
    32581 ctgccttgat gatattttca aaggcttctt tgattatgac gcgctgaatg cctattcaat
    32641 gaagatgacc cgcgatgccg aatacgattt agtgcatgag atggaagcca gcctgatgga
    32701 gttgatgtct tccagtctca agcagcgttt aactgctgag ccggtgcgtt ttgtttatca
    32761 gcgcgatatg cccaatgcgc tggttgaagt gttacgcgaa aaactgacta tttcccgcta
    32821 cgactccatc gtccccggcg gtcgttatca taattttaaa gactttatta atttccccaa
    32881 tgtcggcaaa gccaatctgg tgaacaaacc actgccgcgt ttacgccata tttggtttga
    32941 taaagcccag ttccgcaatg gttttgatgc cattcgcgaa cgcgatgtgt tgctctatta
    33001 tccttatcac acctttgagc atgtgctgga actgctgcgt caggcttcgt tcgacccgag
    33061 cgtactggcg attaaaatta acatttaccg cgtggcgaaa gattcacgca tcatcgactc
    33121 aatgatccac gccgcacata acggtaagaa agtcactgtg gtggttgagt tacaggcgcg
    33181 tttcgacgaa gaagccaaca ttcactgggc gaagcgcctg accgaagcag gcgtgcacgt
    33241 tatcttctct gcgccggggc tgaaaattca cgccaaactg ttcctgattt cacgtaaaga
    33301 aaacggtgaa gtggtccgtt acgcacacat cgggaccggg aactttaacg aaaaaaccgc
    33361 gcgtctttat actgactatt cgttgctgac cgcagatgcg cgtatcacca acgaagtacg
    33421 gcgggtattt aactttattg aaaacccata ccgcccggtg acatttgatt atttaatggt
    33481 gtcaccgcaa aactctcgcc gtctgttata tgaaatggta gaccgcgaaa tcgccaacgc
    33541 gcagcaaggg ctgcccagtg gtatcaccct gaagctaaat aaccttgtcg ataaaggcct
    33601 ggttgatcgt ctgtatgcgg cctccagctc cggcgtaccg gttaatctgc tggttcgcgg
    33661 aatgtgttcg ctgatcccca atctggaagg cattagcgac aacattcgtg ccatcagtat
    33721 tgttgaccgt taccttgaac atgaccgggt ttatattttt gaaaatggcg gcgataaaaa
    33781 ggtctacctt tcttccgccg actggatgac gcgcaatatt gattatcgta ttgaagtggc
    33841 gacaccgctg ctcgatccgc gcctgaagca gcgggtgctg gacatcatcg acatattgtt
    33901 cagcgatacg gtcaaagcac gttatatcga taaagaactc agtaatcgct acgttccccg
    33961 cggcaatcgc cgcaaagtac gggcgcagtt ggcgatttac gactacatca aatcactcga
    34021 acaacctgaa taaccctatg ccaatacacg ataaatcccc tcgtccgcag gagtttgctg
    34081 cggtcgatct tggttcaaac agttttcaca tggtcatagc ccgtgtggta gatggtgcca
    34141 tgcagattat tggccgcctg aaacagcggg tgcatctggc ggacggcctg gggccagata
    34201 atatgttgag tgaagaggca atgacgcgcg gtttgaactg tctgtcgctg tttgccgaac
    34261 ggctacaagg gttttctcct gccagcgtct gtatagtcgg cacccatacg ctgcgtcagg
    34321 cgctgaacgc cactgacttt ctgaaacgcg cggaaaaggt cattccctac ccgattgaaa
    34381 ttatttccgg taatgaagaa gcccgtctga tttttatggg cgtggaacat acccaaccgg
    34441 aaaaaggtcg caaactggtt attgatattg gcggcggatc tacggaactg gtgattggtg
    34501 aaaatttcga acctattctc gttgaaagcc gccggatggg ttgtgtcagc tttgcccagc
    34561 tttattttcc tggcggggtc atcaataaag agaattttca gcgcgctcgc atggcggcag
    34621 cacaaaaact ggaaacttta acctggcaat tccgtattca gggctggaac gttgcaatgg
    34681 gcgcttccgg taccataaaa gccgcccatg aagtgttaat ggaaatgggc gagaaagacg
    34741 ggataattac cccggaacgt ctggaaaaac tggtaaaaga aattttacgt caccgtaatt
    34801 tcgcatcgct gagtttaccg ggtctttccg aagagcggaa aacagtcttc gttcctggac
    34861 tggcgatttt atgtggtgtg tttgatgctt tagccatccg tgaactgcgc ctttctgacg
    34921 gggcgttacg cgaaggcgta ctgtatgaaa tggaaggacg tttccgtcat caggatgtgc
    34981 gtagtcgcac cgccagcagc ctcgccaacc agtatcacat agacagcgaa caggcccgac
    35041 gagtgctgga taccactatg caaatgtacg aacagtggcg ggaacagcaa ccgaagctgg
    35101 cgcatccgca actggaggcg ctactgcgat gggccgccat gctgcatgag gtcgggttga
    35161 atatcaacca cagcggtttg catcgccact ccgcttatat tctgcaaaac agtgacttgc
    35221 cgggttttaa tcaggaacag cagctgatga tggcgacact ggtgcgctat caccgtaaag
    35281 cgattaagct cgacgatctg ccgcgcttta ccttgtttaa gaagaaacag ttcctgccac
    35341 tgatacagct attgcgcctt ggcgtattac taaacaatca acgtcaggca accaccacac
    35401 cgccaacatt gacactgatt actgatgaca gtcactggac actgcgtttc ccgcatgact
    35461 ggtttagtca gaatgcgctg gtactgcttg atctggaaaa ggagcaagaa tactgggaag
    35521 gcgtggctgg ctggcggttg aaaattgaag aagaaagtac cccagaaatc gccgcttaat
    35581 gctttgcggg ccgacgagaa atgtcggccc gcattattca ggcactttcg cgaatgggtt
    35641 cgatttcatt cagcgtatca attaacggtt gcggcttacc aataagataa ccctgcatat
    35701 aatcgatccc caaagagagc accgcctcgc ggatctcttc gttttcaacg tactctgcca
    35761 ctacccgcat tttcttcatt cgcgccagat ggcaaatcga cgccacaatc tgataatcca
    35821 gactatttga cacaatattg cggataaagc tgccgtcaat tttaagcaga tcggcattca
    35881 catttttaag ccgtgcatag ctggcgtagc cggtgccgaa atcatcaatc gcaatctggc
    35941 agcctaattc ctgaagatgc tgcaaggtga tttgcgcctg cttaacattg gtcagagcat
    36001 tactttcggt gacttcaaaa ataagttgcc acgcttcaat ctgatattta gccagcaact
    36061 gactgacttc aacaggaaaa cgagcctgac ataccgaggt tggagacaga ttaatagcaa
    36121 aacggtgagc gggcatcttc gctctgtttt cagccataaa ttgcagcgta tgctcaatga
    36181 cccacatgtc gatactcgac gataaaccaa attcgtgcgc gaccggcaag aagctaccgg
    36241 ggccgatcag ttcatcattc tcacctttca tgcgcagaag aatttcatgg taaacatcac
    36301 cacgcatacc ggtaatcggc tgggccatca ggaaaaaatg gttgtgttcc agcgcctgct
    36361 gtagtcgatt catcatcgcg actttatctt tcaattcgcg ttgcaaatac attgccccgc
    36421 aacgctgcat attttccggg gcgttggtca cgatggaaag ttcggcgacc gtatttagct
    36481 ctcccagcag caggtagata tgattcactg gcgagcgcac atagcagtaa ctgacgccaa
    36541 tctgcggttg catcggcatc ccatcccaaa agaaacgaaa ttgcttgaga tgactatcca
    36601 gtgcggtaat gcgctcctgg tgcgattctg tattcagtcg cagcgcgaga tcgttacccg
    36661 aaagctgata aacatcttca cccggttcca gcaagggtga cagccagtga gaaagttttt
    36721 gcttgt
//
LOCUS       DAZSIO010000023        36597 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZSIO010000023 DAZSIO010000000
VERSION     DAZSIO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.023"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..190
                     /locus_tag="ACHX5T_001035"
     CDS             <1..190
                     /locus_tag="ACHX5T_001035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466323.1"
                     /translation="PPHVKALPDTITQTSIHRTLQFIEFARSCRPDKAFTPHPAINRR
                     RLPNGRFFTYSPPSNVPA"
     gene            complement(156..1391)
                     /locus_tag="ACHX5T_001036"
     CDS             complement(156..1391)
                     /locus_tag="ACHX5T_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR8466324.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="ACHX5T_001037"
     tRNA            complement(1538..1614)
                     /locus_tag="ACHX5T_001037"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="ACHX5T_001038"
     tRNA            complement(1657..1743)
                     /locus_tag="ACHX5T_001038"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="ACHX5T_001039"
     tRNA            complement(1764..1839)
                     /locus_tag="ACHX5T_001039"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="ACHX5T_001040"
     tRNA            complement(1898..1974)
                     /locus_tag="ACHX5T_001040"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="ACHX5T_001041"
     CDS             complement(2077..3462)
                     /locus_tag="ACHX5T_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR8466325.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="wecG"
                     /locus_tag="ACHX5T_001042"
     CDS             complement(3653..4393)
                     /gene="wecG"
                     /locus_tag="ACHX5T_001042"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR8466326.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="ACHX5T_001043"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="ACHX5T_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR8466327.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="ACHX5T_001044"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="ACHX5T_001044"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR8466328.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="ACHX5T_001045"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="ACHX5T_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR8466329.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="ACHX5T_001046"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="ACHX5T_001046"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR8466330.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="ACHX5T_001047"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="ACHX5T_001047"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR8466331.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="ACHX5T_001048"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="ACHX5T_001048"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR8466332.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="ACHX5T_001049"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="ACHX5T_001049"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR8466333.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="ACHX5T_001050"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="ACHX5T_001050"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR8466334.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="ACHX5T_001051"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="ACHX5T_001051"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR8466335.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="ACHX5T_001052"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="ACHX5T_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR8466336.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="ACHX5T_001053"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="ACHX5T_001053"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR8466337.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="ACHX5T_001054"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="ACHX5T_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR8466338.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="ACHX5T_001055"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="ACHX5T_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR8466339.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="ACHX5T_001056"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="ACHX5T_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR8466340.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="ACHX5T_001057"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="ACHX5T_001057"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR8466341.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="ACHX5T_001058"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="ACHX5T_001058"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR8466342.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="ACHX5T_001059"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="ACHX5T_001059"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR8466343.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="ACHX5T_001060"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="ACHX5T_001060"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR8466344.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="ACHX5T_001061"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="ACHX5T_001061"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR8466345.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="ACHX5T_001062"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="ACHX5T_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR8466346.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="ACHX5T_001063"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="ACHX5T_001063"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR8466347.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="ACHX5T_001064"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="ACHX5T_001064"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR8466348.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="ACHX5T_001065"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="ACHX5T_001065"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR8466349.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="ACHX5T_001066"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="ACHX5T_001066"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR8466350.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /gene="ilvG"
                     /locus_tag="ACHX5T_001067"
     CDS             complement(31267..32913)
                     /gene="ilvG"
                     /locus_tag="ACHX5T_001067"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR8466351.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /gene="ilvX"
                     /locus_tag="ACHX5T_001068"
     CDS             complement(32916..32966)
                     /gene="ilvX"
                     /locus_tag="ACHX5T_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR8466352.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="ACHX5T_001069"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="ACHX5T_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR8466353.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..34981
                     /locus_tag="ACHX5T_001070"
                     /pseudo
     CDS             33502..34981
                     /locus_tag="ACHX5T_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="ACHX5T_001071"
     CDS             complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="ACHX5T_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR8466354.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35463..36302
                     /gene="hdfR"
                     /locus_tag="ACHX5T_001072"
     CDS             35463..36302
                     /gene="hdfR"
                     /locus_tag="ACHX5T_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR8466355.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     DVY"
     gene            complement(36398..36473)
                     /locus_tag="ACHX5T_001073"
     tRNA            complement(36398..36473)
                     /locus_tag="ACHX5T_001073"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36438..36440),aa:Trp,seq:cca)
     gene            complement(36482..36558)
                     /locus_tag="ACHX5T_001074"
     tRNA            complement(36482..36558)
                     /locus_tag="ACHX5T_001074"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36522..36524),aa:Asp,seq:gtc)
BASE COUNT         8940 a        10016 c         9220 g         8421 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgctgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgtaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa tgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgtg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 acgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggctc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acggacgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg ggatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccaatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcactgg agatcccatt
    34621 accacccccc ggcattttga gtaaaacggt agtgccggga gaaagcagcg ccaccgttaa
    34681 acaacgcgta atggccgcca gagagcgcca atttaagcgg cagaataaac tgaatgcctg
    34741 gctggatagt ccggaaatac gccaattctg caaacttgag agcgaagatg cgatgtggtt
    34801 ggaagaaacg ctgatccatc tggggttatc gattcgtgcc tggcagcggt tactgaaagt
    34861 tgcacgaacc attgctgata ttgatcagtc tgacattatc acacgtcagc atttgcagga
    34921 ggcagttagc tatcgtgcga ttgaccgttt gctcatccat ctgcaaaaac tactgacata
    34981 aaaaaagggc atttcgccct ttttattaat cgtcagaatc ggtgtagtct tcagcacctt
    35041 caacctgcgg tttaccgccg gaaagggtgt gaaaacgttt tggacgctta atacgcgtca
    35101 tatacttgga ccacacgcgt tctgcttctg tcactggctc acgttcgcca cggcatactg
    35161 ctacgaagag tttctcttcc tcggtaaccg gctcgcgttt gccaagatcc aactcattga
    35221 aggcataacc atgacgctca agcagttgtg cctctttgat ggtgaaatca ccatgacgag
    35281 agaatccacg tggataatgt ttattgtcga aatatcgatt agtcgtcgta aagctttccg
    35341 ccatcctgca cgctcctaat tctttgaccg agctagttat ggcgcggagt attagttacg
    35401 cttgacagag tgtaaaacaa aacatttaaa tcataacgac aaataatttt gcggagagca
    35461 ctgtggatac ggaattgtta aaaactttcc tggaagttag ccgaacgcgt cactttggtc
    35521 gagcggctga atcgctctat ctgacccagt cagcagtgag ctttcgaatc agacaactgg
    35581 aaaatcaact gggtgtgaac cttttcaccc gccacagaaa caatatccgt ttaaccgctg
    35641 ccggtgaaaa actactgcct tatgcagaaa cgctcatgag cacctggcag gccgcccgca
    35701 aggaggtggc gcatacctca cgacataacg agttttctat cggtgccagc gcctcgttgt
    35761 gggaatgtat gcttaatcag tggttgggac gcttgtatca aaatcaggat gcccatacag
    35821 gcttacagtt cgaagcgcga attgcccaac ggcagtctct ggtaaaacag ctgcatgaac
    35881 gccagcttga tcttcttatt accactgaag cgcccaaaat ggacgaattt agtagtcagt
    35941 tgctggggta tttcacttta gcgctttata ccagtgcccc ttctaaacta aagggagatc
    36001 ttaactatct gcgactggag tgggggccag attttcaaca gcatgaggca ggtttgatcg
    36061 gtgctgacga agttcccatt ctgacaacca gttctgctga actggcacag caacagattg
    36121 cgatgcttaa tggttgcacc tggctacccg tcagctgggc gcgtaaaaaa ggcggcctgc
    36181 ataccgttgt cgatagcaca acactttcac ggccgcttta tgccatatgg ctgcaaaata
    36241 gcgataaaaa tgcgttgatt cgcgatcttt tgaaaattaa cgtgctggat gatgtgtatt
    36301 aatctgaatg gctggcaagg atgccggtag aaggatttac ttcggagagg attatttcag
    36361 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg caggggcgga gagactcgaa
    36421 ctcccaacac ccggttttgg agaccggtgc tctaccaatt gaactacgcc cctaattagg
    36481 gtggcggaac ggacgggact cgaacccgcg accccctgcg tgacaggcag gtattctaac
    36541 cgactgaact accgctccac cgaattcttt tacaaccacc ggttttacga ccggctt
//
LOCUS       DAZSIO010000024        34638 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZSIO010000024 DAZSIO010000000
VERSION     DAZSIO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.024"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..1101)
                     /locus_tag="ACHX5T_001075"
     CDS             complement(<1..1101)
                     /locus_tag="ACHX5T_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR8466356.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGAL"
     gene            complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="ACHX5T_001076"
     CDS             complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="ACHX5T_001076"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR8466357.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="ACHX5T_001077"
     CDS             complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="ACHX5T_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR8466358.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="ACHX5T_001078"
     CDS             complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="ACHX5T_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR8466359.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="ACHX5T_001079"
     CDS             complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="ACHX5T_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR8466360.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="ACHX5T_001080"
     CDS             complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="ACHX5T_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR8466361.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3405..4382
                     /gene="pspF"
                     /locus_tag="ACHX5T_001081"
     CDS             3405..4382
                     /gene="pspF"
                     /locus_tag="ACHX5T_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR8466362.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="ACHX5T_001082"
     CDS             complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="ACHX5T_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFR8466363.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="ACHX5T_001083"
     CDS             complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="ACHX5T_001083"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR8466364.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="ACHX5T_001084"
     CDS             complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="ACHX5T_001084"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFR8466365.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="ACHX5T_001085"
     CDS             complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="ACHX5T_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR8466366.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="ACHX5T_001086"
     CDS             complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="ACHX5T_001086"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR8466367.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10284..11702
                     /gene="puuA"
                     /locus_tag="ACHX5T_001087"
     CDS             10284..11702
                     /gene="puuA"
                     /locus_tag="ACHX5T_001087"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR8466368.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11851..12015
                     /locus_tag="ACHX5T_001088"
     CDS             11851..12015
                     /locus_tag="ACHX5T_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466369.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12005..13390
                     /gene="puuP"
                     /locus_tag="ACHX5T_001089"
     CDS             12005..13390
                     /gene="puuP"
                     /locus_tag="ACHX5T_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR8466370.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13524..13769
                     /locus_tag="ACHX5T_001090"
     CDS             13524..13769
                     /locus_tag="ACHX5T_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR8466371.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14082..15725
                     /gene="sapA"
                     /locus_tag="ACHX5T_001091"
     CDS             14082..15725
                     /gene="sapA"
                     /locus_tag="ACHX5T_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR8466372.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15722..16687
                     /gene="sapB"
                     /locus_tag="ACHX5T_001092"
     CDS             15722..16687
                     /gene="sapB"
                     /locus_tag="ACHX5T_001092"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR8466373.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16674..17564
                     /gene="sapC"
                     /locus_tag="ACHX5T_001093"
     CDS             16674..17564
                     /gene="sapC"
                     /locus_tag="ACHX5T_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR8466374.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17564..18556
                     /gene="sapD"
                     /locus_tag="ACHX5T_001094"
     CDS             17564..18556
                     /gene="sapD"
                     /locus_tag="ACHX5T_001094"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR8466375.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18558..19364
                     /gene="sapF"
                     /locus_tag="ACHX5T_001095"
     CDS             18558..19364
                     /gene="sapF"
                     /locus_tag="ACHX5T_001095"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR8466376.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19432..19785
                     /locus_tag="ACHX5T_001096"
     CDS             19432..19785
                     /locus_tag="ACHX5T_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR8466377.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20153..20941
                     /gene="fabI"
                     /locus_tag="ACHX5T_001097"
     CDS             20153..20941
                     /gene="fabI"
                     /locus_tag="ACHX5T_001097"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR8466378.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21085..22212
                     /locus_tag="ACHX5T_001098"
     CDS             21085..22212
                     /locus_tag="ACHX5T_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFR8466379.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22280..24214
                     /gene="rnb"
                     /locus_tag="ACHX5T_001099"
     CDS             22280..24214
                     /gene="rnb"
                     /locus_tag="ACHX5T_001099"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR8466380.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24292..24395)
                     /locus_tag="ACHX5T_001100"
                     /pseudo
     CDS             complement(24292..24395)
                     /locus_tag="ACHX5T_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24449..26423
                     /gene="pdeR"
                     /locus_tag="ACHX5T_001101"
                     /pseudo
     CDS             24449..26423
                     /gene="pdeR"
                     /locus_tag="ACHX5T_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(26405..26533)
                     /gene="ymiD"
                     /locus_tag="ACHX5T_001102"
     CDS             complement(26405..26533)
                     /gene="ymiD"
                     /locus_tag="ACHX5T_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR8466381.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            26571..26744
                     /gene="yciZ"
                     /locus_tag="ACHX5T_001103"
     CDS             26571..26744
                     /gene="yciZ"
                     /locus_tag="ACHX5T_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR8466382.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26834..27583
                     /locus_tag="ACHX5T_001104"
     CDS             26834..27583
                     /locus_tag="ACHX5T_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR8466383.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27852..28070
                     /gene="osmB"
                     /locus_tag="ACHX5T_001105"
     CDS             27852..28070
                     /gene="osmB"
                     /locus_tag="ACHX5T_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR8466384.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="ACHX5T_001106"
     CDS             complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="ACHX5T_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR8466385.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="ACHX5T_001107"
     CDS             complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="ACHX5T_001107"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR8466386.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="ACHX5T_001108"
     CDS             complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="ACHX5T_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR8466387.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="ACHX5T_001109"
     CDS             complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="ACHX5T_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR8466388.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="ACHX5T_001110"
     CDS             complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="ACHX5T_001110"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR8466389.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32019..32609
                     /gene="ribA"
                     /locus_tag="ACHX5T_001111"
     CDS             32019..32609
                     /gene="ribA"
                     /locus_tag="ACHX5T_001111"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR8466390.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32673..>34638)
                     /gene="acnA"
                     /locus_tag="ACHX5T_001112"
     CDS             complement(32673..>34638)
                     /gene="acnA"
                     /locus_tag="ACHX5T_001112"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR8466391.1"
                     /translation="LGQPVSMFIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVV
                     GKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVEL
                     VEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASN
                     ELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAV
                     TLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPD
                     PIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLASE
                     PIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSD
                     TYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDS
                     PAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDS
                     DVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHR
                     SNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVV
                     PCRCRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT         8165 a         9029 c         8746 g         8698 t
ORIGIN      
        1 taatgcacct tcctgctgta atgcctcgac cagcgctaat atttcgcttt ctggaagcaa
       61 gccacgcagt gggttcaacc cgatgccatc gtgagaggcg aggaagttaa accaggtcgt
      121 tttactggag ggcaagctca gactttgcgc ccactggcac agcgcctcga cgttctgttt
      181 ttgcaccgca tgtaacacca gcggcggcag cgagaactgg tacaccatat gcgcttcgtc
      241 atcaccgttg ccaaagtaag caatgttgtc tttgtgcgga acattggttt cggtaataat
      301 cactgtacct ggtgctacgt catcaataat cgaccgtaac agcttgataa tcagatgtgt
      361 tttttccaga tggatgcagc ttgttcccgg ctctttccac ataaagccaa cggcatccag
      421 gcggacatac tcagcacctt tctcaaggta acagagtaaa acatccacca tcgccagcaa
      481 cacttcaggg ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg tccataaatg
      541 gcgcgtggaa ttatcatcca tttgaaacgg cgttaatagc ggtaacgcac gcggaagggt
      601 gacggcactg agatcggttt tcggatcaac cgcaataaag aaatcttcaa aacccggaag
      661 ctgttgtaaa tagtttttaa accattcact ttttgcagac atatggttgc agacaaaatc
      721 aaacattaaa tgactgcatt caccgagttg ctgaatatcc tgccactccc ccgcttcact
      781 ggcgacctga tgataatcaa ttaccgaaaa tccatcatca gatgaccatg ggtaaaaagg
      841 taataaatga acatgtgaaa aaatgctttg cagccattga tggtagaact gattaaatat
      901 gggtagtggt tttaaatcat tactgtgaaa ttgatcggca taggtaatta acacgacatc
      961 actttcatcc cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac tctcaagacg
     1021 cgttacaagt ttctgtaaat gagttgcggt aaatgttcca ccgtagattt cgtccatgta
     1081 atccgtaatt ttctgtttca tgttgccttt ccgtggtagc ggtcccatga ggcgagagac
     1141 tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc aggaaatcat
     1201 tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatag ggatgtaaat
     1261 aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca tggtgaattc ttttaacctt
     1321 tgaccttcgg cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg gtatatccca
     1381 tctcgctaag gagctctttt gcctgcccgg actggcgtcc ggcattgcaa tacactttca
     1441 cggtgtcgtt tttatccgga acggcggtgg caatgcgctc tttcacttcc ttcaggggaa
     1501 tattgatggc cccctgaacg tgctcttgct gatactgctc tggaacacga acatcgatcc
     1561 agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt aagccttttt
     1621 ttaaacatgt aagcgtcctt ctatggatga aaattgccat ccattatacg cctgagattt
     1681 aacaattttt gcgaaactcc cctcaccttt tataacgctg tgccagttta ttagcagccc
     1741 gacttaacag cggttccagc gccacagcca gcaacatttt cagcggtcgg cgagcaactg
     1801 attttatcgc ccagcccgcc acacccgccg ggccatagcg cagtgcggta agaagtacca
     1861 gcttgcctgc taatttgaaa ccaagcttca ccttttgccc ggcctgttgc cagcgagtat
     1921 tcataacttt cctcacagtt gacggaaacg gctacgtaac gtgaaagtat cggaagtgac
     1981 ataacgttcc atctcgcgta aacgcgtttc acttgccgcc agttcacggt cgacttcatc
     2041 cagcaattcg ctgctggaag gtagctgctc accaaaggcc atgttgtccg gcattggatc
     2101 aagcgcaaat gacaaaatga tgtaagcaac cagggtaaac agcgccagac cgaaaaaaat
     2161 cgacagcacc accaggatac gcaccagttt taccggtaca tcaaaatagt tggcaatccc
     2221 ggcgcagacg ccgcggacca tgccctgctg tggaatacgc cataactttt tattgagatt
     2281 aatgcccgcc attagcgatc cctccagttc ggatgttctg catcaagaat agattccagc
     2341 gcctgaatac gttcgcgcat ccgttttgct tcatcaacca gttgcgctaa tcgctgctgc
     2401 tcactttgcg acaattcact gcgaccagaa cgattgctgt aatgcagcca taaccagatc
     2461 ggtaaaacaa acagcacaaa aatggttaac ggaatagcca gaaatagcgc gctcataagt
     2521 actccttaga cgatgagcgg cggcgcgttc ggacgccgcc tggatgttat tattgattgt
     2581 cttgcttcat tttggctttt aattgtgcca gttgttcgct gattgcatca tcggctttca
     2641 gttcggcaaa ctgatcgtcc agcgattttt gtttaccgaa gctgtggctt tctgcttccg
     2701 cttccatctg gtcaatacga cgttcgaaag attcgaaacg agccattgct tcatccagtt
     2761 tgccactgtc cagctgacga cgcacatcgc gcgacgagtt tgccgcctga tgacgtaaca
     2821 tcaatgcctg ctggcgagcg cgtgtttcgc tcaatttgtt ttccagctcg ccaatctctt
     2881 tcttcatgcg tgccagcgta tcgtccacca gcgtcacttc atgttccagg gacttaatca
     2941 gatcggtcag tttctgtttt tcaattaacg ctgcacgtgc cagatcctct ctctctttca
     3001 gcagcgccag ttcggctttt tcctgccatt caacttcacg cgccgacgct tgttcaatac
     3061 ggcgagtcag ctgtttcttt tctgccaacg cacgcgccga agtagaacgt acttcaacca
     3121 gtgtatcttc catctcctgg atcatcagac gcaccagttt ctgtggatct tccgctttct
     3181 ctaacagagc gttgatgttg gcattcacga tgtcggcaaa gcgagaaaaa atacccataa
     3241 tgttgtcctc ttgatttctg cgttcaggat tgtcctgctg aactgttaat acaatttgcg
     3301 tgccaatttt ttatcttttt gatttataaa gatctgattg aagaatcaac agcaacatgc
     3361 caggatgaat tagcgaatta cactaacaag tggcgaattt catcatggcc gaatacaaag
     3421 ataatttact tggtgaggcg aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg
     3481 caccgctgga caaaccggtg ctcatcatcg gcgaacgcgg caccggtaaa gagttgattg
     3541 ccagccgcct gcattatctc tcctcccgtt ggcaagggcc gtttatttcc cttaactgcg
     3601 cggcgttaaa tgaaaatctg ctggattccg aactgtttgg tcacgaagcg ggggcgttta
     3661 ccggtgcgca aaaacgtcat cctgggagat ttgaacgtgc cgacggcggt acgctatttc
     3721 ttgatgaact cgctacggca ccgatgatgg tgcaggaaaa attattgcgt gtgattgagt
     3781 acggtgaact ggagcgcgtt ggcggtagcc agccgttgca ggtgaatgtg cggttggtat
     3841 gcgcgacgaa tgccgatctc ccggcgatgg tcaatgaagg tacttttcgc gctgacctgc
     3901 tcgaccggct ggctttcgat gtagtgcaac tgccgcccct gcgcgagcgc gaaagcgaca
     3961 ttatgctgat ggcagaacac tttgccattc agatgtgtcg ggaaatcaag ctgcctctgt
     4021 tcccggggtt tacggagcgc gccagagaaa cattgctgaa ttatcgttgg ccgggaaata
     4081 ttcgtgaatt gaaaaacgtg gtggaacgtt cagtgtatcg ccacggcacc agcgattatc
     4141 cgcttgatga catcattatt gatcccttta aacggcgtcc acctgaagaa gccatcgccg
     4201 tttcagaaaa tacctcgctt ccgacgctgc cgctggattt acgcgagttt cagatgcagc
     4261 aggaaaaaga gttgctgcaa ctcagtttgc aacaggggaa atataaccag aagcgcgcgg
     4321 ctgaattact ggggttaact tatcatcagt tccgcgcgtt gttgaaaaag caccagattt
     4381 agcgaagttg agcaatacaa cttgccggat gcggcgtgaa tgctttattc ggcatacata
     4441 atcaatacgt cgtttgcagg cctgataagc gcagcgcatc agacattatt gcgttgggct
     4501 attaatcgct cagcgcatcc tgcaaaattt tcattgccgc atcgaattgc gcatccggga
     4561 tggtcagcgg atacaggaag cgaatcacgt tgccgtatgc gccacaggtc agcaggagca
     4621 gcccctgcgc cagcgcgcgt tgctggattt tctgtgcaat cgccgctgac ggctcgcccg
     4681 tttgcggatc gttaaactct gccgcaatca tcgaccccag gccgcgtacc gcagcaatgg
     4741 ccggaacgct ttctttggca tcaatcaacg tgtttttgag acgctggccc agttgattcg
     4801 cgcgttcgca gagtgattct ttgtcgataa tattgagcac cgcgtgcgcg gcagccaccg
     4861 ccagcgggtt cccggcgtag gtgccaccca acccgcccgg cgcgggcgcg tccataatat
     4921 tcgcgttacc gaccacgccc gaaagcggca tcccgcccgc gaggcttttc gccatcgtca
     4981 ttaagtccgg cttatcggcg taatgatcca tggcaaacag tttaccggta cgcgcaaagc
     5041 cgctttgcac ttcatcggca atcatcacaa taccgtgctc gtcgcacagg cggcgaatgg
     5101 cggcaaccag ctcttttggc gcaacgttga aaccgcactc gccctgcacc ggttcgaaaa
     5161 taatggccgc gacctgcttc gcttcgatgt ctgatttaaa caagcgttcg atggcgtcga
     5221 gggagtcctg tgttgaaatg ccgtgtaaat ctgacggata aggtacgtga tacaccgaac
     5281 cagggaacgg gccgaagccg attttgtacg gcgcaacttt tccggtcagc gccatggtca
     5341 tatatgtacg accgtggaag ccaccgctaa acgcaatcac gccaggtcgt ccggtatggg
     5401 cgcgagcaat tttcaccgcg ttttccaccg cttccgcgcc ggtagtgaag aatgcggttt
     5461 tagcctgccc gctcactggg gcaagggtgt taattttctc cgccagcgtg acgtagcttt
     5521 cgtacggcac aatctgatac gccgtgtggg taaactgttg caattgctgc tccaccgccg
     5581 cgaccagatc agggtggcga tgcccggtat tcagcaccgc aatgcctgcg gcgaaatcga
     5641 tgtattcgtt gccctctacg tccttcagcg tagcgttttc tgccgactgg gcgaagaagt
     5701 tacacatcac gccaaccccg cgtggggtag cagaaagacg acgctgatgg aattcattgt
     5761 tgctcatgtt tcacaccttt tgactcttca ctgttaatcg gaaattaaac gcccagacga
     5821 tcgcgcaggc tgtaataagc cgcgcccatc gcggtaaatg gcacgcgcag cgtgcgcccg
     5881 ccggggaacg ggtaatgcgg cagattggcg aaggcatcga aacgctcggc gtcaccgcgc
     5941 agcagttcag caatcaaacg cccggcaaga tgggtacagg tgacgccgtg gccgctgtaa
     6001 ccctgcatgt aatagatgtt ggtatcaagg cggccgaact gcggcatgtg cgacaaggtc
     6061 agcaggaagt tgcccgtcca gcggtaatca attttcacgc ccttcaactg agggaaggtt
     6121 tttagtagtt tcggtaccac aagacgctca acgtcatccg ggttgcgtgc accatacact
     6181 acacccccgc cgtacagcag gcggttgtcg gcggtaagac ggtagtaatc aagcagatag
     6241 ttacagtctt ccacacagta gtttttcggg atcagcgaac gggctaaatc ttccgacagc
     6301 cgttcggtgg tgatcacctg cgtgccgcac ggcatgctgc gtttcgctag ttccggctct
     6361 actttatcgc ccagatacgc attcccggcg acaatcacat acttcgccgt cacctgacca
     6421 ttggcagtcc gaaccacggc tggcgtggtg tgctggatct gcgtcacggc ggaaagctca
     6481 tacacgcgcc cgccgttcag gcggatggca tcggcttcac caatggcgag gtttagcggg
     6541 tgaatatgcc caccgctgtg atccagcagc gcaccagtgt agcgatcgct ggcgacctcg
     6601 cggcgaatag cgttgacgtc cagcagctcc agctgtttat tgccgtagcg tacccagttc
     6661 tctttctgct cttcgagtgt ggcgagttgt ttatcattca tcgccacaaa cagaccgccg
     6721 gggcggtagt cgcagtcaat ctgataacgt ttgatacgtt cgcggatgat ctcgccaccc
     6781 tcgaacatca tgctgccgag catacgggcg gtgtccatgc cgtagctttt ttcgatcacg
     6841 tcgatgtcgc ggctgtagga gttcacaagc tgcccgccat tgcgcccgct tgcgccaaag
     6901 ccgatgcgtg aggcttcgag aaccactacg tcaaagcccg cttccgccag atgcagcgcg
     6961 gaggagagcc cggtatagcc gccgccaacc acgcaaacgt cgcaggtgat tgactcattc
     7021 agtgtgtcga atggtgcata tttattcgca ctggcggcgt agtaactgct ggtatgttcg
     7081 gtcatttcag gcctccaggc ttatccagat ggttttcagt tcagtgaatt tttcaagggc
     7141 atgcagggat ttgtcgcgac cgttgccgct ctgcttatag ccgccaaacg gcacggtcat
     7201 atcgccgtcg ttgtagttat tgacgaagac ggaaccggct ttcaggcgtc ggctcatgcg
     7261 gtgcgcgcgg gagaggtcgc gcgtccatac cgccgcgcca aggccgtact ggctgtcgtt
     7321 ggcaagctgc agcgcctgat cttctgatgt gaaacgggtg accaccagca ccggaccgaa
     7381 aatctcttcg cggcttaagg acgcattcgg gtccacatcc acgaagatgg tcgggccgat
     7441 ggccgctgcc agcccggcgt tgcgaccatc cagcagcagt tgccccttgc cttcgccttc
     7501 acgaataaag gtgtgtaccg agtcagcgtg agcgctgtcg attaaggtgc ccatggtggt
     7561 tgcgggatca agtggatggc ccggctgcca gttttgcgcc tgctgtttta acagggctaa
     7621 gaattcatcg gcgatgctct cttccagtaa caaacgcgtt ccggcgatgc gcacctggcc
     7681 ctggttgtag aagatgcctg cggcggtggc gcttgccgct ttttgcagat ccggacaatc
     7741 ggcgaaaacg atgttggcgc ttttgccgcc cgcttccagc cagacacgtt tcatgttgct
     7801 atcacccgcg tctttcagca gttgtttacc cgtacgcgtg gagccggtaa aggcgatggc
     7861 atcgatatcg ttatgacgcg aaagcgcctg ccccgcttca tgaccaaaac ccgtcaccac
     7921 gttcaacaca ccatccggca agcctgcttc tttcgccagc cccgcgagac gaatcgcact
     7981 gagcggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca gcgccgggcc
     8041 gagtttccag caagtcagca acagcgggaa gttccacggc acgatggcgg caatcacgcc
     8101 gaccggttca cgcacgatca tcgccagctc atggctactg gtggtcgcca cttcgccata
     8161 cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg gaatatcatc
     8221 acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg ccagctcttc
     8281 ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt tagccggaga
     8341 agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg cacggtcgat
     8401 atcgacgctc ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg gatcaacggt
     8461 ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata agcggttttc
     8521 aatggcgaga cttaacgctt tatcctgcca gtaagccaga tgatgaaaat tcattatgac
     8581 tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca gagatccccg
     8641 ggctgcatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg cattgcgctt
     8701 acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct gataattcgg
     8761 caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat ggcataactt
     8821 tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc accttccagt
     8881 actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt gcccggctgg
     8941 tacgtttcaa agatcatcgc cagcgtgcga ttcgggttac cgttatgaac cagcttcatt
     9001 gacacaccct gactgcccat ctcaattaag tcgtcctgat taatgacgac ctgcggctca
     9061 tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac cttcagcagc
     9121 ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact gatagcactg
     9181 tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt
     9241 tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt tccttaaact
     9301 gtagtggtca gagtcgctgt ttttcagcga tatggtgctg ccaagcggtg ataaagccct
     9361 cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc cactgtacgc
     9421 ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc ggcgagcgag
     9481 cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga gagtttaccc
     9541 aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca acctgaactt
     9601 catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga tgttccagca
     9661 attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc accagttctt
     9721 gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca ttaattatcg
     9781 ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt tcaccatata
     9841 ggtgcggctg cacattgctg ggactactag gaagataaat gccatcgagt ttcggcaaaa
     9901 gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc aagccgcctg
     9961 cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga cccttaagcc
    10021 tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc atgctcaatc
    10081 tcacaaaatg gactaaatta tcgccattac tgcgctttaa atgaccttta tgttcaatat
    10141 tttttcaatc tagcagtgga ttttcatttt tgcaaactca atttaacatt tgacaaacat
    10201 ttggtttgca tacagattcg aatgatggtc attatatttt acgctttgat aacgagcgga
    10261 aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag aactttgttc
    10321 agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc tacctggagc
    10381 gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt tgcttccgtg
    10441 gaaaacgtat ccctgtttca agcctgaaga agctcgagaa agggtgttat ttcccggcct
    10501 cggtatttgc aatggatatt ctgggcaacg tagtagaaga agcaggtctg ggtcaggaaa
    10561 tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc cttaactcct tctgccgccg
    10621 atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc gctccctttg
    10681 acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag cgcggattgt
    10741 tcccggttgt agcggtagag ctggagttct atttactgga tcgccagcgc gacgctgaag
    10801 gggatctgca accgccctgc gcgcctggca ccgatgaccg caatacacaa agccaggttt
    10861 actccgttga taacctcaac cacttcgccg acgtgctcaa tgatattgat gaactggcgc
    10921 agttgcagct gattccggca gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa
    10981 tcaaccttta ccatactgat aacgtgctgg aagcctgcga tgatgcgctg gcactaaaac
    11041 gcctagtgcg tctgatggca gaaaagcata agatgcacgc cacttttatg gcgaagccgt
    11101 atgaagagca cgcgggcagc gggatgcata tccatatcag tatgcaaaat aatcgtggcg
    11161 agaatgtgct ttctgacaca gaaggcgaag attcgccgct gctgaaaaag atgctcgccg
    11221 ggatgattga cctgatgccg tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc
    11281 gccgcttcca gccgggaatg tatgtgccga cgcaggcgtc gtggggccat aacaaccgca
    11341 ccgtcgccct gcgtattccg tgcggtgacc gtcataatca ccgcgtggaa tatcacgtgg
    11401 cgggtgccga tgccaaccca tatctggtga tggcagcgat ttttgccggt attttgcatg
    11461 gccttgataa cgagctgccg ttgcaggaag aagtcgaagg caacgggctg gaacaggaag
    11521 gcttaccctt cccgattcgc cagagcgatg ccctgggtga gtttatcgag aatgatcacc
    11581 tgcgccgcta tttaggcgaa cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc
    11641 tgttgcagtt tgagcgcctc atcacagaaa cagaaattga gtggatgttg aaaaacgcgt
    11701 aattcccctt gcggggttgt ttcatcggcc tgagcggagc caggcctgac atttgctggt
    11761 aatccagaca tttcccggat gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa
    11821 cgattaagac tgtgacgagg aaatgagatg atgccgatga ttaaatcccc gcatggcgag
    11881 ggaggctgcg tatgcgcccc tcctgctact gactggactc cgcccccttt gcttcccttg
    11941 ctaaacaggt ttgatttcag gtcgacacga ccgcaaacct tattacgcag gggaggcagc
    12001 aattatggct attaattcac cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg
    12061 aaaatcactg aaattgtggc aggtggtaat gatgggtctg gcctatctca cgccgatgac
    12121 cgtatttgat acttttggta ttgtctccgg cattagcgac ggtcacgttc cggcgtccta
    12181 tttgctggcg ctggcgggcg tgctgtttac cgctatcagc tacggcaaac tggttcgcca
    12241 gtttccggag gccggttcgg cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg
    12301 atttatggtc ggctggtcat cactgctgga ttatctcttt ttgccaatga tcaacgtttt
    12361 gttggcgaaa atctatctct ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt
    12421 aaccttcgtc gccattttaa ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt
    12481 caataccctg tttgtactgg tgcaaatctc catcatggtg gtgtttatct tcctggtggt
    12541 tcagggactg cataaaggag aaggcgttgg caccgtctgg tcacttcagc cgtttatcag
    12601 cgagaacgcg cacctgatcc cgattattac cggtgcgacg attgtctgtt tctcgttcct
    12661 cggtttcgat gcggtgacca cgctttcgga agagacgccg gacgccgcac gcgtgatccc
    12721 gaaagccatc ttcctgacgg cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt
    12781 tatgcagctg ttctttcccg atatcagccg ctttaaagac ccggacgccg cactgcctga
    12841 aattgcgctg tacgtcggcg gcaagctctt tcagtcgatt ttcctctgca ccacgtttgt
    12901 gaacacatta gcgtctggcc tggcttcaca tgccagcgtg tcgcgtctgc tgtatgtgat
    12961 ggggcgtgac aatgtgtttc cggagcgcgt gtttggctat gtgcacccaa aatggcggac
    13021 tccggcactc aacgtcatta tggtcgggat tgtcgcgctg tcggcgctgt tctttgattt
    13081 agtcaccgcg acagcactga ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct
    13141 gtcggtgttt aatcatttct ggcggcgtaa aggaatgaat aaaagctgga aggatcactt
    13201 ccactatttg ctgatgccgc tggttggcgc gctgacggtg ggtgtgctgt gggttaacct
    13261 agagtcaacg tcactgacac tcggtctggt atgggcttcg ttaggcggcg catatttgtg
    13321 gtatctgatc cgccgctatc gcaaagtgcc gctgtacgaa ggtgacagaa cgccggtgag
    13381 cgaaacgtaa tagccggatg cgcggcgcat tatcctgcct gagggtaacg cgccgcgcca
    13441 ttaactataa aaagatgtat gacccggatc acgagtgcta tcatccgccc ttatgcttag
    13501 cgcattaacc ccaggggcaa ataatgaacc acgatatccc gctaaaatat tttgatattg
    13561 ccgatgagta cgcgaccgaa tgcgcagagc ctgtcgcaga ggctgaacgc acaccgctgg
    13621 cccactactt ccagttactg ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag
    13681 cccaacatga aatggctgct gaagcgggga ttaatcccgt acgcattgat gagatcgcag
    13741 agtttttgaa tcaatggggt aatgagtaat tatttaattg cagacgatcg gtcagtatcg
    13801 ttgtccggat aaagcgttca cgccgcatcc ggctaacaat gacacgcact gcagcgtgct
    13861 ttaatgggtt tctcacgatc gtaccgctct gctcctcgct aagttgttct gtgagttata
    13921 gttatagaga aatcacgggt acgaatgttg aacagatggc gacgccacct gttcaacgac
    13981 caaccacacg gccctcatta cgacccctga acacaaacgg taatggcaaa tgtcattaaa
    14041 tattagtaag atagcctctt tacataaaat cccttaatat tatgcgccag gtattatcgt
    14101 ctcttttggt gattgctgga cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc
    14161 cgcatgctga tatccgcgac agcggttttg tctattgcgt cagcgggcaa gtcaacacct
    14221 ttaacccatc caaagcgagc agtgggttaa ttgtcgatac ccttgccgcc cagttttatg
    14281 atcgactgct ggatgtcgat ccctatacct atcgcctgat gccggaactt gccgaaagct
    14341 gggaagtact cgacaacggc gcgacctatc gcttccacct gcgtcgcgat gttccgtttc
    14401 aaaaaaccga ctggtttact cccactcgta aaatgaatgc cgacgatgtg gtgtttacct
    14461 tccagcgaat ttttgaccgc aacaacccgt ggcataacgt caacggcagc aacttcccct
    14521 acttcgacag cctgcaattt gccgataacg tgaaaagcgt ccgcaaactg gataatcata
    14581 ccgttgagtt ccgactcgcc cagccggatg cttctttttt gtggcacctc gcaacccatt
    14641 atgcttcggt catgtcggca gaatatgccc ggaagttaga gaaagaagat cgccaggagc
    14701 aactcgaccg tcaaccggtc ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc
    14761 aatttattcg cctacaacgt catgatgact tctggcgcgg taaaccgtta atgccgcagg
    14821 tagtggtgga tttaggctcc ggcggcaccg gacgtctgtc gaaactcctg accggggaat
    14881 gcgacgttct ggcctggcct gctgccagcc agctatccat tttgcgtgac gacccgcgct
    14941 tgcgtttaac gctgcgtcct gggatgaacg tcgcctatct ggcatttaac accgccaaac
    15001 cgccgctaaa taatcccgct gtccgccatg cactggcact ggcgattaat aaccagcgcc
    15061 tgatgcaatc catctattat ggtacggctg aaacggcggc ctctatttta ccgcgcgcct
    15121 cgtgggccta tgacaacgag gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac
    15181 agttgaaggc gttggggctg gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc
    15241 aggcgtggaa ccccagtcca ctgaaaactg ccgaactgat tcaggcggat atggcgcagg
    15301 ttggcgtaaa agtggtgatt gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg
    15361 atatgagcca tgatctgacg ttatccggtt gggcgacgga cagtaacgac ccggacagtt
    15421 tcttccgtcc gttactgagc tgcgcggcaa ttcattcaca gaccaacctc gcccactggt
    15481 gcaatccgaa attcgacagc gtgttgcgta aggcgctctc ctcgcagcag ctggcagcgc
    15541 gtattgaagc ctatgacgaa gcgcagagta ttctggcgca ggaattacct attctgccgc
    15601 tggcgtcgtc attgcgtttg caggcctatc ggtacgatat caaaggtctg gtacttagcc
    15661 cgtttggtaa cgcctccttt gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac
    15721 catgattatc ttcaccttac gccgcatttt gttattgatt gtcaccttgt tcctgctgac
    15781 cttcgttggc ttcagcttga gctatttcac tccacacgcg ccgttacaag gcgcgtcact
    15841 gtggaatgcc tgggtgttct ggtttaatgg cctgatccac tgggattttg gtgtttccag
    15901 cattaacggt caaccaattg ccgaacagtt aaaagagctc ttcccggcga cgatagaact
    15961 gtgcatcctc gccttcggtt ttgcgctgat tgttggcatc cccgtgggga tgattgccgg
    16021 gattacgcgc cataagtggc aggataactt gatcaacgcc atcgccttac tgggcttttc
    16081 aatcccggtg ttctggctgg cgcttctgtt gacactgttt tgttcactca cgctaggttg
    16141 gttgcccgtt tcagggcgtt tcgatctgct ctacgaagtg aaaccgatta ccggttttgc
    16201 gttgattgat gcctggcttt cggactcacc atggcgggat gaaatgatca tgagcgcaat
    16261 ccgccatatg atattgcccg tgatcaccct gtcggttgcg ccaacaactg aagtgatccg
    16321 cctgatgcgt atcagcacta ttgaagtgta tgaccagaac tatgtaaaag cggcggcgac
    16381 ccgcggtttg tcgcgcttta ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc
    16441 ggttattcct cgcctgggct tacagttttc caccatgctg acgctggcga tgatcaccga
    16501 aatggttttt agctggccgg gtttagggcg ctggttaatt aacgccatcc gccagcagga
    16561 ttacgcagcc atttccgccg gagtgatggt gtgtggctca ctggtcatta ttgttaacgt
    16621 gatttctgat attttgggtg ccatggctaa ccctctgaaa cataaggaat ggtatgcctt
    16681 acgatagcgt atacagcgaa aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat
    16741 tttatagtga cgcctctgcg atggtcggcc tgtacggctg cgcgggactg gctgtactgt
    16801 gtatttttgg cggctggttt gcgccttacg gcatcgacca gcaatttctc ggttatcaat
    16861 tattgccgcc atcatggtcg cgctatggcg aagtttcttt cttcctgggg actgacgacc
    16921 tcggacgtga tgttttaagc cgtttactga gcggagctgc gccaaccgtg ggtggcgcat
    16981 ttgtggtgac gcttgccgcg acgatctgtg gcctggtact gggcaccttc gccggggcga
    17041 cacacggcct ccgctcagcg gtgcttaacc atattctgga taccttactg gcgatccctt
    17101 cgctgctgtt ggcaattatc gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt
    17161 ttgctgtctg gctggcattg ctgccgcgta tggtacgttc gatttacagc atggtgcatg
    17221 acgaactgga aaaagagtac gttatcgccg cccgtctgga tggcgcatca acgctgaata
    17281 ttctctggtt tgctgtgatg ccaaacatca ccgccgggct ggtcaccgag atcacccgcg
    17341 cactgtcgat ggcaattctc gatatcgccg cgctgggctt tctcgatctc ggcgcacaac
    17401 tcccctcgcc tgaatgggga gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc
    17461 cgtggactgt catgctgcca ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc
    17521 ttggcgatgg cgttcgtcgt gcgattattg cgggggtgga ataatgccat tactcgatat
    17581 tcgtaacctg accattgaat ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt
    17641 aagtatgacc ttaaccgaag gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa
    17701 gagtttgatt gcgaaagcga tttgtggggt gaataaagat aactggcgtg ttactgctga
    17761 ccgtatgcgt tttgatgata tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact
    17821 ggttggccat aacgtgtcga tgattttcca ggaaccgcag tcgtgtcttg acccttcaga
    17881 acgtgtgggt cgccagttga tgcaaaacat cccagcctgg acctataaag gccgttggtg
    17941 gcagcgtttt ggctggcgca aacgccgtgc cattgaactg ctgcaccgcg tggggattaa
    18001 agatcacaaa gatgcgatgc gcagttttcc ctatgagttg accgaaggtg aatgtcagaa
    18061 agtgatgata gccattgcac tggcgaatca accgcgtctg ctgattgctg acgaaccgac
    18121 aaactcaatg gagccaacaa cccaggcgca aatctttcgc ctgctgacgc gtctcaacca
    18181 aaacagcaat accactattt tgcttatcag ccatgactta caaatgctta gccaatgggc
    18241 ggataaaatt aacgtgcttt actgcggtca aacagtggaa accgcgccga gtaaggagtt
    18301 ggtgacgatg ccacatcatc cttataccca ggcgctgatc cgcgcgatac cagacttcgg
    18361 cagcgcgatg ccgcataaaa gtcgcctcaa tactctaccc ggcgctatcc cattgctgga
    18421 acagttaccg attggttgtc gtctggggcc acgttgcccg tatgcacaac gagaatgcat
    18481 tgtgacgcca cgtttgacgg gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa
    18541 catggagaaa gagtgagatg atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt
    18601 tccgctaccg gaccggttgg tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct
    18661 ttacgctacg tgaaggccag acactggcga ttattggcga gaatggttcg ggtaaatcca
    18721 cactggcaaa aatgctggcg ggaatgatag aacctaccag cggcgagtta ttgattgacg
    18781 atcatccgct gcattttggc gattattcct tccgtagtca gcgtattcgc atgatttttc
    18841 aggatccttc gacctcgttg aatccccgtc aacgtatctc acaaattctg gattttccac
    18901 tgcgcctgaa caccgacctg gaaccggaac agcgtcgcaa acagattatt gaaacgatgc
    18961 gtatggttgg gctgctaccg gatcacgtca gttactatcc gcatatgttg gcacccggac
    19021 aaaaacagcg tctgggtctg gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg
    19081 atgaagccct cgcttcactg gatatgtcaa tgcgttcgca gttgattaat ctgatgctgg
    19141 agttacagga aaaacagggc atttcgtata tttatgttac ccagcatatc ggaatgatga
    19201 agcacattag cgaccaggtg ctggtgatgc atcagggcga ggttgtcgag cgaggcagca
    19261 ccgcggatgt gctggcatcg ccgctgcatg aactcaccaa acggctgatt gccgggcact
    19321 ttggcgaagc attgacagcg gatgcgtggc gtaaagatcg ctaattttct gaatcctcct
    19381 tccagattat tcacctcatt ctgacaagag attaaatctt gtcagaatga ggtgatggat
    19441 aaaataaaat caaatgcgcg tgatttaaga cgcaatctca ctttgcagga acgaaagctc
    19501 tggcgatatc ttcgcagccg acgttttggt gatttcaaat ttcgccgtca acatccggtg
    19561 gggagctaca ttctcgattt tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt
    19621 gggcagcatg atttggcagt tgcctatgat accaggcgca ctagctggct tgagtcgcag
    19681 ggctggaccg tgctgcgttt ctggaataac gagattgatt gtaatgagga ggcggtgctg
    19741 gagattattc tgcaggaact gaaccgccgg tcaccctcac cctgaaaggg cgagggggcc
    19801 gaccgagccg aatagctgtt gtggtgaaaa catggagaca gtgctggaga atattcggca
    19861 aggtctgaac catcccagcc atcgccataa aagggttagg ggctgtatga gcctgtttgt
    19921 tgctggggta acaatatttg cacaatatgg tcccctcgcc cctctgggga gagggttagg
    19981 gtaaggggaa aagcgccccc cctgccgcag cctgctccgg tcggacctgg taactatagc
    20041 tactcacagc caggttgatt ataataaccg tttatctgtt cgtactgttt actaaaacga
    20101 cgaatcgcct gattttcagg cacaacaagc atcaacaata aggattaaag ctatgggttt
    20161 tctttccggt aagcgcattc tggtaaccgg tgttgccagc aaactgtcca tcgcctacgg
    20221 tatcgctcag gcgatgcacc gcgaaggagc tgaactggca ttcacctacc agaacgacaa
    20281 actgaaaggc cgcgtagaag aatttgccgc tcaattgggt tctgacatcg ttctgcagtg
    20341 cgatgttgca gaagatgcca gcatcgacac catgttcgct gaactgggga aagtttggcc
    20401 gaaatttgac ggtttcgtac actctattgg ttttgcacct ggcgatcagc tggatggtga
    20461 ctatgttaac gccgttaccc gtgaaggctt caaaattgcc cacgacatca gctcctacag
    20521 cttcgttgca atggcaaaag cttgccgctc catgctgaat ccgggttctg ccctgctgac
    20581 cctttcctac cttggcgctg agcgcgctat cccgaactac aacgttatgg gtctggcaaa
    20641 agcgtctctg gaagcgaacg tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg
    20701 tgttaacgcc atctctgctg gtccgatccg tactctggcg gcctccggta tcaaagactt
    20761 ccgcaaaatg ctggctcatt gcgaagccgt taccccgatt cgccgtacag ttactattga
    20821 agatgtgggt aactctgcgg cattcctgtg ctccgatctc tctgccggta tctccggtga
    20881 agtagtccac gttgacggcg gtttcagcat cgctgcaatg aacgaactcg aactgaaata
    20941 atcgttctgt tggtaaagat gggcggcgtt ctgccgcccg ttatctctgt tatacctttc
    21001 tgatatttgt tatcgccgat ccgtctttct ccccttcccg ccttgcgtca ggataacgat
    21061 ttcctttacg accaaggagc gcccatggaa caacgccaca tcaccggcaa aagccactgg
    21121 tatcatgaaa cgcaatccag tactacggag tatgacgttc tgcctctggt cccggaagcc
    21181 gcaaaggtca gcgatccctt tctgctcgac gtgatccttg atgaagagac gctggccccc
    21241 ttcctttcat ggctggtccc tgcgcgcgtt cttgcagtgg agttgttccc tgaccagctt
    21301 accgtgaccc gttcacaaac cttcaccgct tatgaacgct tgtcgacggc cctgacggtt
    21361 gctcaggttt gcggcgtcca gcggttatgt aactactatt cggcgcgact tacgccgctc
    21421 cccggacctg attcctccag ggaaagtaat catcggttgg cacaaatcac gcaatatgcc
    21481 cgccaactgg ctagctcgcc ttctattatc gacaaccgat cgcgccagca tctgaatgac
    21541 gtcggtctta ctgtctggga ctgtgtgatc attaaccaaa tcattggttt tattggcttt
    21601 caggcgcgga caattgcgac atttcaggct tatctcgggc atccggtacg ctggttaccc
    21661 gggctggcga tacaaaacta cgccgacgcg tcactgtttg ctgatgaatc aatacgctgg
    21721 cgaagcagct atgaggtgga aaaactacct gaagagcaca caaaaagttc aactgcagaa
    21781 ctttgccaac tggccgaaac actctctctc caccctattt cactttccct tctcgaaaag
    21841 ttgttaaaca gcacacgggt caatgcacag ccggataatc agcttgcggc gttgttatgc
    21901 gcgcgtataa atggcagtcc tgcttgtttt tccacctgta tggattcatc aaatgaatat
    21961 aaaaaaatca gcacccttct gcgcaagggc gaaaatgaaa ttaaccgatg ggctgaccgt
    22021 cattctgttg agcgcgctac cgttcaggcg atacaatggc tgacccgagc acccgatcgc
    22081 tttagcgccg cccagttcag ccctttactc gaacacgaaa aatcatcaac gcagattatt
    22141 aatctgctgg tatggagcgg gctgtgtggc tggataaatc gcttaaaaat cgcgttgggt
    22201 gagacatatt aaccttgccg cgtcagacag attcgcgtaa aactgtcagc cgctctaatg
    22261 gccaccaaaa tagacaatta tgtttcagga caacccgctg ctagcgcagc ttaaacagca
    22321 actgcattcc cagacgccac gcgctgaagg ggtggtaaaa gccacagaaa aaggctttgg
    22381 cttcctggaa gtcgacgcac aaaaaagtta tttcattccg ccgccgcaga tgaaaaaagt
    22441 catgcatggc gaccgaatta tcgcggtgat ccacagtgaa aaagaacgtg aatccgcaga
    22501 gccagaagaa ctggttgaac cgttcctgac tcgtttcgtg ggtaaagttc agggcaaaaa
    22561 tgaccgtctg accatcgttc ctgatcatcc acttttaaaa gacgccattc cttgccgcgc
    22621 agcccgtggc ctgaaccacg agtttaaaga aggcgactgg gcggttgccg aaatgcgccg
    22681 tcatccgctg aaaggcgatc gttctttcta tgctgaactg acacaataca tcacttttgg
    22741 tgacgaccac tttgtaccgt ggtgggttac ccttgcgcgc cataatctgg aaaaagaagc
    22801 accagacggc gtcgctaccg aaatgctcga tgaaggtctg gttcgtgaag atctgacctc
    22861 gctggatttt gtcaccatcg acagtgccag cactgaagat atggatgacg cccttttcgc
    22921 taaggcgctg ccggatgaca aacttcagct gattgtggcg attgccgatc caaccgcgtg
    22981 gattgctgaa ggcagcaagc tggacaaagc cgcgaaaatt cgcgcattca ccaactatct
    23041 gcctggcttc aacatcccta tgctgcctcg cgagctttct gacgatctct gctcactgcg
    23101 cgccaatgaa gtccgcccgg tactggcatg ccgcatgacg ctctccgctg atggcaccat
    23161 tgaagataat atcgaattct ttgccgccac catcgaatcc aaagcgaagc tggtgtatga
    23221 ccaggtttct gactggctgg aaaataccgg tgactggaag cctgaaagtg aagcaattgc
    23281 cgaacaagtc cgtttgctag cgcaaatttg ccaacgccgc ggcgagtggc gtcataacca
    23341 cgcactggtg tttaaagatc gcccggatta ccgctttatt ctcggtgaaa aaggtgaagt
    23401 gctggatatc gtcgccgagc ctcgtcgcat tgccaaccgt atcgtcgaag aagcgatgat
    23461 tgccgctaac atttgtgcgg cccgcgtact gcgcgataag ctcggttttg gcatctataa
    23521 cgtgcatatg ggctttgatc cggcgaatgc cgacgcgctg gcagcgttgc tgaaaacgca
    23581 cggtctgcat gtcgatgccg aagaagtgct cacgctggac ggtttctgca aactgcgtcg
    23641 tgaactggat gcgcaaccaa ctggtttcct cgacagccgc attcgtcgct tccagtcatt
    23701 tgctgaaatt agctctgaac ccggtcctca ctttggcctc ggtctggaag catacgccac
    23761 ctggacttcg ccgatccgta aatatggcga catgatcaac caccgtctgc tgaaagcggt
    23821 tatcaaaggc gaaactgcga cgcgtccgca ggatgaaatc actgtccaaa tggccgaacg
    23881 tcgccgtctc aaccggatgg cagaacgtga tgttggcgac tggttatacg cacgcttcct
    23941 gaaagataaa gccgggaccg gcacccgttt cgcggcggaa attgtcgata tcagccgtgg
    24001 cggcatgcgt gtgcgtttgg ttgataacgg cgctatcgcc tttattccgg cacctttctt
    24061 acacgctgtg cgcgatgaac tggtttgcag ccaggaaaac ggcactgtac aaattaaagg
    24121 tgaaacggtt tacaaagtaa ctgacgttat tgacgtcacc attgccgaag tccgcatgga
    24181 aacccgcagc attattgcgc gcccggtcgc gtaatctcct ttcacggccc attcctcatg
    24241 gatgggccgt ttatttcccc gctctacctt cgttatatcc aggcagattt tttaactgac
    24301 tttcgtttga aaactggcgt ttttccaaaa ctggattaca aataaatata taaaaaaaca
    24361 atgcgtttag ccgccgttaa tttcatgtaa gtcatgttgt tccttttatt taaccgcgga
    24421 ctccgctgtt aaccggagga tatgcatcat gaaaaccgtt agggagtcca caacgttgta
    24481 caactttctc ggatcgcaca atccatactg gcggttgacg gaaagcagcg atgctttgcg
    24541 cttttctacc accaaaacca cagaacctga tcgtacattg cagttatctg ccgaacaggc
    24601 tgctcgcatc agggaaatga cggtcatcac ctccagcctg atgatgagtc tgaccgtcga
    24661 tgaaagcgat ctttctgtgc atctggtagg acgaaaaatc aataaacggg aatgggctgg
    24721 caacgcgtct gcatggcatg acacaccggc ggttgctcgt gatttatcac acgggctttc
    24781 ctttgctgag caggtagttt ctgaagcaca ttccgcaata gtgattctcg acagccgggg
    24841 gaatatccaa cgcttcaatc ggttatgtga agattacaca gggttgaaag aacacgacgt
    24901 cattgggtaa agcgtgttta aactgtttat gagccgtcgt gaagctgcgg catccaggcg
    24961 caataaccgt gtattttttc gaagcggcaa tgcatatgaa gtcgaactgt ggataccaac
    25021 acgtaaaggc cagcggctgt ttctgtttcg caataaattt gtccacagcg gcagtggcaa
    25081 aaacgagatt tttttaatct gttccggtac cgacattacc gaagagcgcc gcactcagga
    25141 gcgactgcgt attctggcaa ataccgacag tatcaccgga ctgccgaatc gtaacgcaat
    25201 gcaggagtta atcgatcacg ctattaatca ggcagataac aataaagttg gggttgtgta
    25261 tcttgatttg gataatttta aaaaggtcaa cgacgcctat gggcatttgt ttggtgacca
    25321 gttattacgc gacgtgtcat tggctatttt aagctgtctc gaacatgacc aggtgttggc
    25381 gcgtccaggt ggcgatgagt ttctggtact ggcatccaat acctcacaaa gcgcgctgga
    25441 agcaatggca tcacgaattt tgacccgctt acgcctcccc tttcgtattg gtttaattga
    25501 agtttatacc agctgttcag taggtatttc gctctctccc gaacatggtt cggacagcgc
    25561 gactattatt cgtcacgccg acacagcaat gtacacagcg aaggaaggcg gacgaggaca
    25621 attttgcgtt tttactccag aaatgaatca gcgggtattt gaatatctct ggctggatac
    25681 caacttgcgt aaagcactgg aaaacgatca gttggttatc gaaaatcacc tagcgtgtcg
    25741 aagtgcgcag tctggaagca ctagtacgtt ggcagtcacc tgaacgtggg ttgattccac
    25801 cgttggactt catttcctac gccgaagagt cagggctaat tgtgccttta ggccgttggg
    25861 tgattctcga tgtcgtacgc caggtggcaa agtggcggga taaaggcata aacctgcgag
    25921 tggcggtaaa tatttctgca cgtcagctcg ccgatcaaac cattttcacc gccctgaaac
    25981 aggttctcca ggaactcaat tttgaatact gccctataga tgttgaactg acagagagtt
    26041 gtctgattga gaatgatgaa ctggcactgt ctgttattca acaatttagc caactaggtg
    26101 cgcaagtgca tctggatgat tttggtaccg gctactcttc actttcgcaa ctggcgcgct
    26161 ttccgatcga tgccatcaaa cttgaccagg tttttgttcg agatattcac aaacaacctg
    26221 tctcgcagtc actggtccgg gcgatcgtcg ctgtggccca ggcattgaat cttcaggtga
    26281 tcgccgaagg tgtagagagt gcaaaggaag atgctttttt aaccaagaac gggatcaatg
    26341 agcggcaagg atttttgttt gccaaaccga tgcccgccgt cgccttcgaa cgctggtata
    26401 aacgctatct gaagcgcgca taagtagatg aatatatata cttagcgcta tatcccgtca
    26461 ttatatatcg tattcctgga tgataattag ccgttgctgt taaacacttc tcttttagcg
    26521 gcaatttttg cattattcat tttccccctt ttgctataag gatgtccttg atgtctgaat
    26581 ttgatgccca gcgcgttgcc gaacgtattg atattgtgtt agatatcctt gtggcaggcg
    26641 attaccactc tgcgatccat aatctcgaga tacttaaagc agaactcttg cgccaggttg
    26701 ctgaatctac gccagacatc ccgaagacac cgtgggagat ttaacaatcg tgccttcagg
    26761 ttttcctatt tgaggatgcc cggcgcgtca gtattttttt atttagtatt ataacgttat
    26821 aagaattaca gcgatgaatt cccgacaaca aactattcta cagatggtca ttgaccaggg
    26881 tcaggttagc gtaaccgatc tggcaaaagc cactggagtt tctgaagtta ccattcgcca
    26941 ggatctcaac accctcgaaa aactgagtta cctccgccgt gcacatggct ttgcagtttc
    27001 gcttgatagt gatgacgtcg aaacccgtat gatgagcaac tatacgctga agcgtgaact
    27061 cgccgagttt gccgcgtcac tggttcaacc gggcgaaacc atctttatcg aaaatggcag
    27121 cagcaatgcc ctgctcgccc ggactctggg ggagcagaag aaaaatgtca ctatcatcac
    27181 ggtcagcagc tacatcgcgc atttgctgaa agacgcgcct tgtgaagtca ttttgctcgg
    27241 tggcgtgtac cagaaaaaaa gcgaaagtat ggttggccct ttgacacgcc agtgcatcca
    27301 gcaggtacat ttcagcaaag catttattgg tattgatggc tggcaacctg aaactggatt
    27361 caccggtcgc gatatgatgc gcaccgatgt ggtcaatgcc gtgctggaaa aagagtgcga
    27421 agcgatagtc ctgactgaca gctcgaaatt tggtgctgta cattcatact ccatcggtcc
    27481 cgttgagcga ttcaatcgcg tgattaccga ttcgaaaata cgcgccagcg atctgatgca
    27541 tcttgagcac agtaaactca ccgttcacgt cgttgacatt taaccatttc ccttctctac
    27601 ggatgatttg cagtttggca aatcatccgc tctaagatga ttcctggttg ataattaaga
    27661 ctatttacct gttattaaca ctctcaagat ataaaattat tatcagcgat ataacaggaa
    27721 gtaattatca cctgcgtgat ataaccctgc gcgcgagcag atttcacgga ataatttcac
    27781 caggcttatt cttagctatt atagttatag agagcttact tccgtgaatc ataaattcag
    27841 gagagagtat tatgtttgta acgagcaaaa aaatgaccgc ggctgttctg gcaattactt
    27901 tggcaatgtc tctgagtgcc tgttctaact ggtctaaacg ggaccgcaac accgcaatcg
    27961 gcgcgggtgc aggggcatta ggcggtgcag tactgaccga tggcagtacg ttgggtacat
    28021 taggtggtgc agctgtcggt ggtgttattg gtcaccaggt cggtaaataa tcgtaaataa
    28081 atgtcgatta tcgactcgca attataatac gcttgattct gttcagtacg aaataactga
    28141 ataatacaca cagtaaaata ttcgaaagcc acggatatat ccgtggcttt ttatgttaac
    28201 cgcctgcgag ttttaccttc atccctttcg cttcaagtag tgattttaat aaatcacgct
    28261 tatcgccctg gatttcaata actccatctt taactgctcc gccgcagccg cattttttct
    28321 tcagttccgc tgcaagtttt gtcagttcgg catcatcgag atcgataccg gtaatcaggc
    28381 aaacgccctt acctttacgt ccactggtct gacgctgaat acgcaccgct ccatcgcctt
    28441 tagggcgtac tggggccgct ttgggctcat caatacgccc ggtttccgtt gagtagacca
    28501 gacggctgtt ggaatcactc atcatgcact ccgctgtaaa gaggcgttga tcgctttcag
    28561 tgtctgcgct ggatctaccg attgcgttac cgggcgacca atcaccatat aatcaacacc
    28621 agccgccaac gcctgttctg gcgtcataat gcggcgctgg tcaccagctt cactcccctg
    28681 cggacgaatg cccggtgtaa ccagtttgaa ctcctgaccg aatacctgtt taaagcgcac
    28741 agcttcctga gcagaacaca ccacaccatc aaggtcacat ttttgcgtca gtgctgccag
    28801 acgttctgca taatctgcag gtgacagtgt cacgccaaga tcggccaggt cgctggcttc
    28861 catgctggtc aacactgtca cagcaatcaa aagcggtgca tctttgccaa acggaaccag
    28921 tgcctcacgc gctgcggtca tcatacgcgc cccaccggag gcatgaacat tcaccatcca
    28981 cacgcctaag tcagctgcag cagcgacagc gtgcgctgca gtgttgggga tatcgtggaa
    29041 tttcaggtca agaaagatat caaaaccacg ctgttgaagt tcgcgcacaa actgtggccc
    29101 aaacaatgta aacatctctt taccgacctt cagacgacaa tcgcgtgggt cgatcttgtc
    29161 gacaaaggac agcgcgtcat cacaattatg ataatcaagg gcaacaacca caggagaatt
    29221 cgtaacggcg cgggaagaag atgaagcagt taacgtcatg accagacctt cttgatgatg
    29281 ggcaccggga gtggcgcgaa acaggtaaac ggcgagcatt ctacccgtga aaataaaaaa
    29341 ttaacagttg cgatttttcc cctagcaggc tttttctgct ccgtcgttgc cggagcacaa
    29401 tgggacatca ataactagta tgttgtaact aaagtcggat ttttttaaaa attacaggcc
    29461 atcaagaccg cgaatcggtt taatagttga ccaggcacga caagacggac aatgccagta
    29521 gagggtgtat gcggtaaaac cacatttctg gcagctataa cgaggcttac tacgcacctt
    29581 ctcgccaacc atgtcacgca gcaccatcag actctctttg gcgcgccctt cttccgcttc
    29641 atttaagtgg taatccatta gcttatggaa tacacgcatg gtcggatgac gctgaagctg
    29701 gcgtgtaata tagacctgtg cagcctcact accgtcgcgc gcttcgatga tatcagcaag
    29761 catcaattca gcatcggcac cggtgttctc ttccaccgcg cgttgcagga attctgccca
    29821 ttcggcagtt ttacccaact gctgatagca ggtttgcaac atttccagcg tttcgctgac
    29881 cagttctctg tcctgggata tgacgcgttg caaactttcg acggctttgg cgtattctcc
    29941 tttcgccata aacacgcgtc ccatcatgat ggatacgcgg gcgctgtttt tatctgccgc
    30001 cgcccctttt tttagcaagg tcatggcacg atcgagatcg tcgctggcca tatgctgcag
    30061 ggctaactca cagtagaaat gggcaatttc gacgcgctgt ttatctttac ccagcttcac
    30121 caggcgttcg gcaacatcaa ttgctttctg ccactcactg gtagcctggt agatttgtag
    30181 caactgttgc agcgcgccaa tgcggaagtc agtttcatcg gtcagctgat tgaacatgtc
    30241 ttccgcgcgg tcgtataacc cggcggccat gtaatcacgc cccagttgtt gaatcgccaa
    30301 cagacgctgt tcataggtca gcgaggcgct ttccattagg gtctgatgga tgcgaatagc
    30361 gcgatcaact tcgccacgcg aacggaacag gtttccgagc gtaaggtggg cttcaacggt
    30421 gcctgtatcc tctttaagca tatcgagaaa caggtctacc gctttatcct gttgattact
    30481 aagcaggaag ttaacccccg ctacgtaatc acgcgacaag cggttggctt catcttgctt
    30541 gttttgttgc gcacttctgc ggcccatata ccagccatag gcagcggcta caggcaaaag
    30601 cagaaacaac aactccagca tagaaagtta ttccttcgcc gctgacgagt gcggcactac
    30661 agccacgtca gtcgcgggtg aaagctggtt ttccagtcgc tttattttac gttcagcgcg
    30721 cgccagggaa acacgaactc gcagccagaa caggccacaa atcaaccaac cgatagcaaa
    30781 ccccgcagca aacaataccg ccagcaatgt ggaaatacgg tactcccctt gcgctaacag
    30841 ataattaaac gtcacctgtt gatcgttctg cgcacccaac gtgaccgaaa tcacgaagat
    30901 cgctaacacc agtaagaaaa tgagtaaata tttcacatta cttcccgtta tgtggttaga
    30961 gcgaataaat tatggtcaac ttaccgcaat agccctttaa agtaccattt tcgcgccgtc
    31021 tgcgaaatgg catccacgcg cattgttgat gatttaaggc ctaaattcgg aaaatcaacc
    31081 agcgttaact ttcttgttct cgttgtgcaa tttcgcgatt ttcttccgca ggtggtgtta
    31141 atggcccaca aattcgttgc gcaagccacg ttgccaccgc caccagcgcc cacgaaatca
    31201 acgtagctac caccagatcg cgtggccaat gcatcccgag cagcaggcgg cttcccatga
    31261 ctcccgttgc ccagaccaac aagatagcaa tggttaacgt tcgccgacgc ggccacagca
    31321 aaccaacggc cagcagtgcc caactggcag caaacatcgt gtgaccggaa ggaaaggcaa
    31381 accccgtctc tttctgccag tgtgaacgca aatattgtgg aatatttttc tcttcagcca
    31441 actgttcttt tactagattt ccgcgttctg ctcgctttaa agtgtagaac tcatcaaccg
    31501 gaatatgatg tgttttttcc aaccagataa caaaaggtcg tggttcctgt actttgtctt
    31561 tgatccagga tttaacgcct tgtccaacaa ggattgccgc cgccagaatg gcaaataata
    31621 caaaggcagc cttaatgcga aaacgcagac accagagaaa ccagccgaac aaaatcaaat
    31681 gtgtaatgac gccccagggc tgggtgacag tttcagtaac ccaaaaagcc gcttttagta
    31741 gccaactttg ttctccaggt tgccaacgcc agccagaaat ccatacggct actggcatga
    31801 caagcaatag tgcagctccc actgcggtac gtcgggcaat cgaacgcatg gcctctcctt
    31861 tttgataagt cccacaatca taactgaaaa tgccagttcc aagaaaaatt gacagatttg
    31921 tgccattccg tgaacgatcg acgcgtcgtg attaggtgaa ccccttctcg ttatggcaaa
    31981 ataagccaat acagaaccag cattatctgg agaatttcat gcagcttaaa cgtgtggcag
    32041 aagccaaact gccaacccca tggggcgatt tcctgatggt gggatttgaa gaactggcaa
    32101 ccggacacga tcatgttgcg ctagtctatg gcgatatttc cgggcatacc ccggtacttg
    32161 cgcgcgtcca ttccgaatgt ctgaccggtg acgccctgtt cagcttgcgc tgcgattgtg
    32221 gcttccagct cgaagcggca ttgacgcaaa ttgccgagga aggccgtggt attttgctgt
    32281 atcaccgtca ggaaggtcgt aacattggtc tgctgaataa aatccgcgct tacgcactgc
    32341 aggatcaagg ttacgatacc gtagaggcta accaccagtt aggcttcgcc gctgatgagc
    32401 gcgacttcac tctttgcgct gatatgttca aactccttgg cgtcaatgaa gtccgcttgt
    32461 taaccaataa cccgaaaaaa gtcgaaattc tgaccgaagc agggattaat attgttgaac
    32521 gcgtaccatt gattgtaggt cgtaacccca ataacgaaca ttatctcgat accaaagccg
    32581 agaaaatggg ccatttgctg aacaaataac cctcttgcat cgtgtaattc atttgcttgc
    32641 cggaagcaaa ataaccggca agcaaatact tgttacttca acatattacg aatgacataa
    32701 tgcaaaatgc cgtcgttctg gtagtaggtc aactccgtcg cggtgtcgat acgacaacgg
    32761 cagggtacga cttcctggct accatccgcg cgcgtaagcg tcaccggaac cgtcgcgcca
    32821 ggttgtagat tttgcagatc gccaatatca atcttctctt ccccggttag ccctaacgtt
    32881 ttacgcgtca cgccttgcgg aaattccagc ggcaggatgc ccatgccaat taaattcgaa
    32941 cggtgaattc gttcaaacga ttcggcaatc accacacgaa ttccaagcag acgaggtcct
    33001 tttgccgccc agtcacggct tgagcctgat ccatactctt tcccggcaat caccgccagc
    33061 ggcgtttgct cctgcttata gcgcatcgca gcatcataaa tagagactac gtcgctgtca
    33121 ggtaaatgcc gcgtcatccc cccttcaacg ccaggcacca tttcattacg gatgcgaata
    33181 ttggcgaagg tgccgcgcat catcacttca tggttaccac gccgcgaacc gtaggagtta
    33241 aagtcttttc gctcaacacc ccgaccttgt agatatcgac ccgctgggct gtcgggctta
    33301 atactgcccg ccggagagat atggtcagtg gtgactgaat cccccagcat tgcgaggatc
    33361 cgcgcaccgt gaatatcttc cactggtgct ggtgttgcct gcatttcatc aaagaaaggc
    33421 gataagcgaa tataggttga gtcctcctgc caaccgtagg tatcggatcg tgtgacgtta
    33481 atttccttcc actctgctgt gccttcaaaa acttctgcgt actctttgcg gaacatttct
    33541 gtggagactt gttctaccgc acgggcaatt tcttgtgccg atggccagat atctttcaga
    33601 taaaccggct cgcctttgcg atcatggccg ataggctcag aagccaggtt gatattcata
    33661 tttcccgcca gcgcataggc aaccaccagc ggtggcgagg ccagccagtt agttttaacc
    33721 agcggatgga tacggccttc aaagttacgg ttgccggaca gcaccgcacc gacggttaaa
    33781 tcgccttttt tgattgccgt ttcgatagga tcgggcagcg gcccagagtt accaatacag
    33841 gtggtacaac cgtatcccac aaggttaaac cccagttcgt cgagatacgg tgtcagtttc
    33901 gcttttgcca gataatcaga aacgactttc gaacccggtg ccaacgacgc tttgacccac
    33961 ggttgccgct tgaggccaag agttacggct tttttcgcca gcaagcctgc ggccatcagc
    34021 acacttgggt tagaggtgtt ggtgcacgag gttatcgcag caatgaccac agcgccatca
    34081 ggtaactgat actgatgtcc gttcataaca taatcgactg gcaggcgatc tttatgcgtg
    34141 gcattcactt tcagttcgtt actggcggca aatgcttttg gtacatcggg cagtgcaacg
    34201 cgatcctgtg ggcgttttgg ccctgccagg ctcgcttcaa cgtcattcat atccagttct
    34261 aacatactgg taaaaatcgg ttcatcgccc gggttacgcc acatgccctg cgctttggca
    34321 tatttttcga ccaactcgac ctgatcttcg ctgcgcccgc ttaaacgcat gtaatcgagg
    34381 gttactgcat cgattgggaa gaagccacag gtggcaccat attctggcga catattggca
    34441 atggtggcgc gatccgccaa cggtagtgaa tccagaccat caccataaaa ttcgacgaat
    34501 ttccccacca cgccatgttt gcgcagcatt tgggtaacag tgagaaccag gtctgtggcg
    34561 gtaatacctt cacgtaattt tccggtaagt ttaaagccca ctacgtccgg gataaacatg
    34621 gaaaccggct ggcctaac
//
LOCUS       DAZSIO010000025        33714 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZSIO010000025 DAZSIO010000000
VERSION     DAZSIO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.025"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(116..592)
                     /locus_tag="ACHX5T_001113"
     CDS             complement(116..592)
                     /locus_tag="ACHX5T_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR8466392.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(651..1940)
                     /gene="bioA"
                     /locus_tag="ACHX5T_001114"
     CDS             complement(651..1940)
                     /gene="bioA"
                     /locus_tag="ACHX5T_001114"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR8466393.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            2027..3067
                     /gene="bioB"
                     /locus_tag="ACHX5T_001115"
     CDS             2027..3067
                     /gene="bioB"
                     /locus_tag="ACHX5T_001115"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR8466394.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            3064..4218
                     /gene="bioF"
                     /locus_tag="ACHX5T_001116"
     CDS             3064..4218
                     /gene="bioF"
                     /locus_tag="ACHX5T_001116"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR8466395.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            4205..4960
                     /gene="bioC"
                     /locus_tag="ACHX5T_001117"
     CDS             4205..4960
                     /gene="bioC"
                     /locus_tag="ACHX5T_001117"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR8466396.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            4953..5630
                     /gene="bioD"
                     /locus_tag="ACHX5T_001118"
     CDS             4953..5630
                     /gene="bioD"
                     /locus_tag="ACHX5T_001118"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR8466397.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            6209..8230
                     /gene="uvrB"
                     /locus_tag="ACHX5T_001119"
     CDS             6209..8230
                     /gene="uvrB"
                     /locus_tag="ACHX5T_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR8466398.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(8422..9330)
                     /gene="yvcK"
                     /locus_tag="ACHX5T_001120"
     CDS             complement(8422..9330)
                     /gene="yvcK"
                     /locus_tag="ACHX5T_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR8466399.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      9595..9739
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            9727..10716
                     /gene="moaA"
                     /locus_tag="ACHX5T_001121"
     CDS             9727..10716
                     /gene="moaA"
                     /locus_tag="ACHX5T_001121"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR8466400.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            10738..11250
                     /gene="moaB"
                     /locus_tag="ACHX5T_001122"
     CDS             10738..11250
                     /gene="moaB"
                     /locus_tag="ACHX5T_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR8466401.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            11253..11738
                     /gene="moaC"
                     /locus_tag="ACHX5T_001123"
     CDS             11253..11738
                     /gene="moaC"
                     /locus_tag="ACHX5T_001123"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR8466402.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            11731..11976
                     /gene="moaD"
                     /locus_tag="ACHX5T_001124"
     CDS             11731..11976
                     /gene="moaD"
                     /locus_tag="ACHX5T_001124"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR8466403.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            11978..12430
                     /gene="moaE"
                     /locus_tag="ACHX5T_001125"
     CDS             11978..12430
                     /gene="moaE"
                     /locus_tag="ACHX5T_001125"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR8466404.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            12567..13271
                     /locus_tag="ACHX5T_001126"
     CDS             12567..13271
                     /locus_tag="ACHX5T_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR8466405.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            13476..14189
                     /locus_tag="ACHX5T_001127"
     CDS             13476..14189
                     /locus_tag="ACHX5T_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR8466406.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(14225..15181)
                     /locus_tag="ACHX5T_001128"
     CDS             complement(14225..15181)
                     /locus_tag="ACHX5T_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFR8466407.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(15181..16422)
                     /gene="clsB"
                     /locus_tag="ACHX5T_001129"
     CDS             complement(15181..16422)
                     /gene="clsB"
                     /locus_tag="ACHX5T_001129"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR8466408.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(16419..17180)
                     /locus_tag="ACHX5T_001130"
     CDS             complement(16419..17180)
                     /locus_tag="ACHX5T_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR8466409.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            17313..17723
                     /locus_tag="ACHX5T_001131"
     CDS             17313..17723
                     /locus_tag="ACHX5T_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR8466410.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(17685..18791)
                     /locus_tag="ACHX5T_001132"
     CDS             complement(17685..18791)
                     /locus_tag="ACHX5T_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8466411.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVLFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(18802..19935)
                     /locus_tag="ACHX5T_001133"
     CDS             complement(18802..19935)
                     /locus_tag="ACHX5T_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8466412.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(19928..21664)
                     /locus_tag="ACHX5T_001134"
     CDS             complement(19928..21664)
                     /locus_tag="ACHX5T_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR8466413.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(21657..22652)
                     /gene="hlyD"
                     /locus_tag="ACHX5T_001135"
     CDS             complement(21657..22652)
                     /gene="hlyD"
                     /locus_tag="ACHX5T_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR8466414.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(22655..23326)
                     /gene="cecR"
                     /locus_tag="ACHX5T_001136"
                     /pseudo
     CDS             complement(22655..23326)
                     /gene="cecR"
                     /locus_tag="ACHX5T_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
     gene            23555..24775
                     /gene="rhlE"
                     /locus_tag="ACHX5T_001137"
     CDS             23555..24775
                     /gene="rhlE"
                     /locus_tag="ACHX5T_001137"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR8466415.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSVAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGIRLMNKRKTA"
     gene            complement(24769..24867)
                     /locus_tag="ACHX5T_001138"
                     /pseudo
     CDS             complement(24769..24867)
                     /locus_tag="ACHX5T_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24949..25431)
                     /gene="ybiA"
                     /locus_tag="ACHX5T_001139"
     CDS             complement(24949..25431)
                     /gene="ybiA"
                     /locus_tag="ACHX5T_001139"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR8466416.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            25551..27701
                     /gene="dinG"
                     /locus_tag="ACHX5T_001140"
     CDS             25551..27701
                     /gene="dinG"
                     /locus_tag="ACHX5T_001140"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR8466417.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            27729..28691
                     /gene="ybiB"
                     /locus_tag="ACHX5T_001141"
     CDS             27729..28691
                     /gene="ybiB"
                     /locus_tag="ACHX5T_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR8466418.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            28832..29917
                     /gene="hcxB"
                     /locus_tag="ACHX5T_001142"
     CDS             28832..29917
                     /gene="hcxB"
                     /locus_tag="ACHX5T_001142"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR8466419.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            29984..30043
                     /gene="ybiE"
                     /locus_tag="ACHX5T_001143"
     CDS             29984..30043
                     /gene="ybiE"
                     /locus_tag="ACHX5T_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR8466420.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(30146..30406)
                     /gene="ybiJ"
                     /locus_tag="ACHX5T_001144"
     CDS             complement(30146..30406)
                     /gene="ybiJ"
                     /locus_tag="ACHX5T_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR8466421.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASPLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(30671..30937)
                     /gene="ybiI"
                     /locus_tag="ACHX5T_001145"
     CDS             complement(30671..30937)
                     /gene="ybiI"
                     /locus_tag="ACHX5T_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR8466422.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(31011..31688)
                     /gene="ybiX"
                     /locus_tag="ACHX5T_001146"
     CDS             complement(31011..31688)
                     /gene="ybiX"
                     /locus_tag="ACHX5T_001146"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR8466423.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(31730..33714)
                     /locus_tag="ACHX5T_001147"
                     /pseudo
     CDS             complement(31730..33714)
                     /locus_tag="ACHX5T_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         7936 a         8755 c         9119 g         7904 t
ORIGIN      
        1 ttctcggtca gcttttttat actaacttga gcgaaacggg aaggtaaaaa gacaaaaagt
       61 tgtttttaat acctttaagt gataccagat ggcattgcgc catctggcag agtgattaac
      121 taaacatcgc ggtaatcgat gcgcttgcca gagagtggaa atgaacgtta aacccgacca
      181 tcgcgccgct ggctccttca tcgacatcaa tacgctctac atccagcgcg tgaacggtaa
      241 aaatgtagcg atgggtttcg cctttcggcg gcgctgcgcc atcgtatccg gttttaccaa
      301 aatcggtacg cgtctgcaaa acgccgtctg gcattgctac cagaccagag ccaaaccctt
      361 gcggtaatac gcgggtatca gcgggtaaat taacaactac ccagtgccac cagccggagc
      421 cggttggcgc atccgggtcg tagcaggtga caacaaaact tttcgttccc gcaggaacat
      481 catcccacgc cagatgcggt gaaatattat cgccatcgta acccatgccg ttaaagacat
      541 gacgatgcgg caacttatcg ccatcgcgca gatcgttact gatgagtttc atgaaccctc
      601 ctttcttgtt tgcagaaagt gtagccagaa accctcacgc tgacttcccg ttattggcaa
      661 aaaaatgttt catcctgtac cgcgcggtta accgctgcgg tcagacgctg caactgttgc
      721 gggagaataa tatagggcgg catcaggtaa atcagtttgc caaaaggccg gatccatacc
      781 ccctgttcga caaagaattt ttgcagcgcc gccatattca ccggacgagt ggtttcgacc
      841 acaccgattg cccccagtac gcgcacatcg gcaaccattt cggcatcacg ggctggtgcc
      901 agttgctcgc gcagctgcac ttcaatagcc gccacctgct gctgccattc gccggattcg
      961 ataatcgcta ggctggcgtt tgctgccgcg caggccagcg gattgcccat aaaagttggc
     1021 ccatgcataa agcagccggc ttcgccgtta ctgatggttt ctgcaacctc gcgcgtggta
     1081 agtgtggcgg aaagggtcat tgtgccgccg gttaaggctt taccgaggca caaaatgtcc
     1141 ggcgcgattt ctgcatattc acaggcaaac agtttgccgg tacgaccaaa tccggtggcg
     1201 atctcgtcgg caatcagcaa gataccttcg cgatcgcaca ttttgcggat tcgttttaac
     1261 cattccggat ggtacatgcg catcccgcct gcgccctgga caatcggctc aatgatcacc
     1321 gctgcgattt catgacgatg cgccgccatc aggcgggcaa aaccaaccat atcgcgttca
     1381 tcccattcac catccatgcg gctttgcggg gcgggagcaa acaagttttc tggcagatag
     1441 cctttccaca ggctgtgcat tgagttgtcc ggatcgcaca ccgacatcgc gccaaaggta
     1501 tcgccatgat aaccattgcg gaaggtcaga aaacgctggc gcgcttcgcc tttggcttgc
     1561 cagtactgca acgccatttt catcgccact tccaccgcta cggaaccgga gtccgcgaga
     1621 aaaacgcact ccagcggttg cggcgtcatt gccaccagtt tgcggcatag ctcgatggcg
     1681 ggcgcatggg tgataccgcc aaacatcaca tgcgacatgg catcaatttg cgtcttcatc
     1741 gccgcattaa gctgcgggtg attgtagccg tggatcgccg cccaccagga cgacataccg
     1801 tcaaccaggc gtctgccgtc agacaaaatt agctcgcaac cttcggcgct cgccaccgga
     1861 taaaccggca gaggggaggt catggatgtg tatgggtgcc agatatggcg ttggtcaaag
     1921 gcaagatcgt ccgttgtcat aatcgacttg taaaccaaat tgaaaagatt taggtttaca
     1981 agcctacacc gaattaacaa caaaaaacac gttttggaga agccccatgg ctcaccgccc
     2041 acgctggaca ttgtcgcaag tcacagaatt atttgaaaaa ccgttgctgg atctgctgtt
     2101 tgaagcgcag caggtgcatc gccagcattt cgaacctcgt caggtgcagg tcagcacgtt
     2161 gctgtcgatt aagaccggag cttgtccgga agattgcaaa tactgcccgc aaagctcgcg
     2221 ctacaaaacc gggctggaag ccgagcggtt gatggaagtt gaacaggtgc tggagtcggc
     2281 gcgcaaagcg aaagcggcag gatcgacgcg cttctgtatg ggcgcggcat ggaagaatcc
     2341 ccacgaacgc gatatgccgt acctggaaca aatggtgcag ggggtaaaag cgatggggct
     2401 ggaggcgtgt atgacgctgg gcacgttgag tgaatctcag gcgcagcgcc tcgcgaacgc
     2461 cgggctggat tactacaacc acaacctgga cacctcgccg gagttttacg gcaatatcat
     2521 caccacacgc acttatcagg aacgcctcga tacgctggaa aaagtgcgcg atgccgggat
     2581 caaagtctgc tcaggtggca ttgtgggctt aggcgaaacg gtaaaagatc gcgccggatt
     2641 attgctgcaa ctggcaaacc tgccgacgcc gccggaaagc gtaccaatca acatgctggt
     2701 gaaggtgaaa ggcacgccgc ttgccgataa cgatgacgtt gatgcctttg attttattcg
     2761 caccattgcc gtagcgcgga tcatgatgcc gacctcttac gtgcgccttt ctgccggacg
     2821 cgagcagatg aacgaacaga ctcaggcgat gtgctttatg gcaggcgcaa actcgatttt
     2881 ctacggttgc aaactgctga ccacgccgaa tccggaagaa gataaagacc tgcaactgtt
     2941 ccgcaaactg gggctaaatc cgcagcaaac tgccgtgctg gcaggggata acgaacaaca
     3001 gcaacgtctt ggacaggcgc tgatgacccc ggacaccgac gaatattaca acgcggcagc
     3061 attatgagct ggcaggagaa aatcaacgcg gcgctcgatg cgcggcgtgc tgccgatgcc
     3121 ctgcgtcgcc gttatccggt ggcgcaagga gccggacgct ggctggtggc ggatgatcgc
     3181 cagtatctga acttttccag taacgattat ttaggtttaa gccatcatcc gcaaattatc
     3241 cgtgcctgga agcagggggc ggagcaattt ggcatcggta gcggcggctc cggtcacgtc
     3301 agcggttata gcgtggtgca tcaggcgctg gaagaagaac tggccgagtg gcttggctat
     3361 tcgcgggcgc tgctgtttat ctctggtttt gccgcgaatc aggcagtcat tgccgcgatg
     3421 atggcgaaag aggaccgtat tgttgccgac cggcttagcc atgcctcatt gctggaggct
     3481 gccagtttaa gcccgtcgca gcttcgccgt tttactcata acgatgtcgc tcatctggcg
     3541 cgactgcttg cttccccctg tccggggcag caactggtgg tgacagaagg cgtgttcagc
     3601 atggacggcg atagcgcgcc actggcggaa atccagcagg taacgcaaca gcacaatggc
     3661 tggttgatgg tcgatgatgc ccacggcacg ggcgttatcg gggagcaggg gcgcggcagc
     3721 tgctggctgc aaaaggtaaa accagaattg ctggtagtga cttttggcaa aggatttggc
     3781 gtcagtgggg ctgcggtgct ttgctcaagt acggtggcgg attatctgct gcaattcgcc
     3841 cgccacctta tctacagcac cagtatgccg cccgctcagg cgcaggcatt acgtgcgtcg
     3901 ctggcggtca ttcgccgtga tgagggggat gcacggcgcg aaaaactggt gtcactcatt
     3961 gcgcgttttc gtgccggagt acaggatttg ccgtttacgc tggctgattc atgcagcgcc
     4021 atccagccat tgatcgtcgg tgataacagc cgtgcgttac aactggcaga aaaactgcgc
     4081 cagcaaggct gctgggtcac ggcgattcgc ccgccaaccg tacccgctgg tactgcgcga
     4141 ctgcgcttaa cgctaaccgc tgcgcatgaa atgcaggata tcgaccgtct gctggaggtg
     4201 ctgcatggca acggttaata aacaagccat tgcagcggca tttggtcggg cagccgcaca
     4261 ctatgagcaa catgcagatc tacagcgcca gagtgctgac gccttactgg caatgcttcc
     4321 acagcgtaaa tacacccacg tactggacgc gggttgtgga cctggctgga tgagccgcca
     4381 ctggcgggaa cgtcacgcgc aggtgacggc gttagatctc tcgccgccaa tgcttgcgca
     4441 agcccgccat aaagatgccg cagatcatta tctggcagga gatatcgaat ccctgccgtt
     4501 agcgactgcg acgttcgatc ttgcatggag caatctcgcg gtgcagtggt gcggtaattt
     4561 atccacggca ctccgcgagc tgtatcgggt ggtgcgcccc ggaggcgtgg tcgcgtttac
     4621 cacgctggtg cagggatcgt tacccgaact gcatcaggcg cggcaggcgg tggacgagcg
     4681 tccgcatgct aatcgctttt taccgccaga tgaaatcgaa gagtcgctga acggcgtgcg
     4741 ttatcaacat catattcagc tcatcacact atggtttgat gatgcgctca gtgccatgcg
     4801 ttcgctgaaa ggcatcggtg ctacgcatct tcatgaaggg cgcgacccgc gaatattaac
     4861 gcgttcgcag ttgcagcgat tgcaactggc ctggccgcaa caacaggggc gatatcctct
     4921 gacgtatcat ctttttttgg gagtgattgc tcgtgagtaa acgttatttt gtcaccggaa
     4981 cggataccga agtggggaaa accgtcgcca gttgtgcact tttacaagcc gcgaaggcag
     5041 taggttatcg gacggcaggt tataaaccgg tcgcctctgg cagcgaaaag accccggaag
     5101 gcttacgtaa tagtgacgcg ctggcgttac agcacaacag cagcctgcag ctggattacg
     5161 caacggtaaa tccttacacc ttcgcagaat ccacctcgcc gcacatcatc agcgcccaag
     5221 agggcaggcc gatagaatca ttggtaatga gcgccggatt acgcgcgctt gaacaacagg
     5281 ctgactgggt gttagtggaa ggtgctggcg gctggtttac gccgctttct gacactttca
     5341 cttttgcaga ttgggtaaca caggaacaac tgccggtgat actggtagtt ggtgtgaaac
     5401 tcggctgtat taatcacgcg atgttgactg cgcaggcaat acaacacgcc ggactgactc
     5461 tggcgggttg ggtggcgaac gatgttacgc ctccgggaaa acgtcacgct gaatatatga
     5521 ccacgctcac ccgcatgatt cccgcgccgc tgctgggaga gatcccctgg cttgcagaaa
     5581 atccagaaaa tgcggcaacc ggaaagtaca taaaccttgc cttgttgtag ccattctgta
     5641 tttggttaaa ttgcgagcga gatcgcgtct tcgattgact gcaatttaac caattaaatt
     5701 ctaaaataat cacgaaaaaa attttacttc cgcctcatgc ggcgaatgtg ggaattgccc
     5761 aggcggcggg ggataggggc tggagacagt tatccactat tcctgtggat aaccatgtgt
     5821 attagagtta gaaaacacga ggcaagcgag agaatacgcg gcttgcacgc gaattggcgt
     5881 taaagacggc tcaaagaaat atcttttatt ttttaactgg ttagataaat gcaatggcag
     5941 tcactgaaca ggcatctctt gccataaaac tgtcatcact catcttgaca aatgttaaaa
     6001 aagccgttgc tttggggata acccggtaag gctggagttt tatctcgcca cagagtaaat
     6061 tttgctcatg attgacagcg gagtttacgc tgtatcagaa atattatggt gatgaactgt
     6121 ttttttatcc agtataattt gttggcataa ttaagtacga cgagtaaaat tacatacctg
     6181 cccgcccaac tccttcaggt agcgactcat gagtaaaccg ttcaaactga attccgcttt
     6241 taaaccttct ggcgatcagc cagaggcgat tcgacgtctc gaagaggggc tggaagatgg
     6301 cctggcgcac cagacgttac ttggcgtgac tggctccggg aaaaccttca ccattgccaa
     6361 tgtcattgct gaccttcagc gcccaaccat ggtacttgcg cccaacaaaa cgctggcggc
     6421 ccagctgtat ggcgaaatga aagagttctt cccggaaaac gcggtggagt atttcgtctc
     6481 ctactacgac tactatcagc cggaagccta tgtaccgagt tccgacacct tcattgagaa
     6541 agatgcctcg gttaacgaac atattgagca gatgcgtttg tccgccacca aagcgatgct
     6601 ggagcggcgt gatgtgattg tagtggcgtc tgtttccgcg atttatggtc tgggcgatcc
     6661 tgatttatat ctcaagatga tgctccatct cacggtcggt atgattatcg atcagcgcgc
     6721 gattctgcgc cgactggcgg agctgcaata cgctcgtaat gatcaagcat tccagcgtgg
     6781 tactttccgc gttcgtggcg aggtgataga tatcttcccg gcagaatcgg atgacattgc
     6841 acttcgcgtg gaactgtttg acgaggaagt ggaacgattg tcgttatttg acccgctgac
     6901 cgggcagatt gtttccacta ttccacgttt taccatctac ccgaaaacgc actacgtcac
     6961 accgcgcgag cgcatcgtac aggcgatgga ggagatcaaa gaagaactgg ccgccagacg
     7021 caaagtgctg ttggaaaaca acaaactgct ggaagagcag cggctgaccc agcgtaccca
     7081 gtttgatctg gagatgatgg acgagctggg ctactgttcg gggattgaaa actactcgcg
     7141 cttcctctcc ggtcgtggac cgggtgagcc accgccgacg ctgtttgatt acctgcctgc
     7201 cgatgggctg ctggtcgtcg atgaatctca cgtcaccatt ccacaaattg gcggcatgta
     7261 tcgcggtgac cgggcgcgta aagagacact ggtggagtac ggcttccgcc tgccatcagc
     7321 gctggataac cgtccgctta agtttgaaga gttcgaagca ttagcgccgc aaaccatcta
     7381 tgtttcggcg acgccgggta attacgagct ggaaaaatcc ggcggcgatg tggtggatca
     7441 ggtggtgcgt ccaaccggat tgcttgaccc gattatcgaa gtgcggccgg tggcgacaca
     7501 ggttgatgat cttctttcgg agattcgtca gcgagcggca attaacgaac gcgtgctggt
     7561 caccacactg accaagcgaa tggcggaaga tcttaccgaa tatctcgaag aacacggtga
     7621 gcgcgtgcgt tatcttcact cagatatcga caccgtcgag cgtatggaga ttatccgcga
     7681 cttgcgtctg ggtgagttcg acgtattggt agggatcaac ttactgcgtg aaggtctgga
     7741 tatgccggaa gtgtcgctgg tggcgatcct cgacgctgac aaagaaggct tcctgcgttc
     7801 cgaacgttcg ttgatccaga ccattggtcg tgcggcacgt aacgttaacg gtaaagcgat
     7861 tctctacggc gataggagca ctccatcaat ggcgaaagcg attggcgaaa ccgaacgtcg
     7921 ccgtgagaaa cagcagaagt acaacgagga acacggcatt acgccgcaag gcttgaacaa
     7981 gaaagtggtc gatatcctgg cgctggggca gaacattgcc aaaaccaaag cgaagggcag
     8041 aggaaaatcg cgcccgattg ttgagccgga taatgtgccg atggatatgt tgccgaaagc
     8101 gttgcagcag aaaatccatg aactggaagg gctgatgatg caacacgcgc agaatctgga
     8161 gttcgaagaa gcggcacaaa ttcgtgacca gttgcatcag ttgcgtgagt tgtttattgc
     8221 cgcatcgtaa caggatagcg aagaagactg atgacaaacg gaaaacagtc tgatgcgcta
     8281 cgcttatcag gcctacattt tctccgcaat atattgaatt tgcgcggttt gtaggccgga
     8341 taaggcgttc acgccgcatc cggcataaac aacgcgcact ttgtcttcaa taggaagccg
     8401 gaattttcct tcggatttcc gttaacctaa agcctgtaac gccttttcca gcgcgttatg
     8461 taacaactgg cggtcatgac gatacggaat atcgctggcc tccagtacct cctggatcac
     8521 aatccgctct ttcaccgccg agacatccac ttttggcccg acgatgaccg catcaatgac
     8581 ctttttacca acatactgct ccataattgc cagcttgctt tccagcttca aattagccgc
     8641 aggtaaactc aactcacgcc ccagattgcc gatataaacc atcggcgctg gcgtgcggcg
     8701 taatgcctgg gcgatttcct tcagcagcag aattggcatc aggctggtat aaaaactgcc
     8761 aggcccaata atgatgagat ccgcttcatt gatagcgtga accgcctcac gcgttgcggg
     8821 tacattaggc gttaacaata actcttgaat cggcgtagtt aactggtcga tattgacctc
     8881 gccgtaaact tcatgcccct gatcgtcaat cgccatcaga tcaacaggat gctctgacat
     8941 tggaatcaaa tgcgtatcca ctttcagcag attacgaatt aaattgatgg cttccagagg
     9001 ccgcacgcta aggtgatcca gcgcctttaa catcaagttt ccgagattat gaccggaaag
     9061 ttcgccattg ccgccaaaac ggtattcaaa catcgcggag gcgacgttcg gttccgttat
     9121 cagctggttg aggcagttgc gcatatcgcc ccaagcaatg ccgccttctg aacggcgaat
     9181 acgccccgtc gagccaccat tatccgtggt ggtgacgata cccgttaaac gagaacccaa
     9241 agacgaaagt gatgagagaa cgcgtcccag tccatgccct ccgccgagag caacgacacg
     9301 atcaagatca gccagcgtac gattgcgcat atagtttcct ggagtcagat tatccgcgct
     9361 acagtagcgc aaattgcggg gaaacagcaa ttaacctgcc aatattgagg atacaaacta
     9421 ttttcttcta tctctaaagg acgatgcacg ctatgcctcc ctgatgatgt atatcaaagt
     9481 aaaaccgcca ttttccctta ttctgtagcg aaatagcacg atcatgacgc tatatacatg
     9541 attacatagc gaaagtgtgg atggtaaaaa tctcatttac acgctagtat cggcttaacc
     9601 actaaacact ctagcctctg cacctgggtc aactgatacg gtgctttggc cgtgacaatg
     9661 ctcgtaaaga ttgccaccag ggcgaaggaa gaaatgactt cgcctcccgt atctggaaag
     9721 gtgtacatgg cttcacaact gactgatgta tttgcgcgta agttttacta cttgcgcctg
     9781 tcgattaccg atgtgtgtaa ctttcgttgc acctactgcc tgccggatgg ctacaaaccg
     9841 agcggcgtca ccaataaagg ctttcttacc gtcgatgaaa ttcgccgggt tacgcgcgcc
     9901 ttcgccagtc tgggcaccga aaaagtgcgc ctgacaggag gagagccgtc tttacgccgt
     9961 gactttaccg atatcatcgc cgctgtgcgg gaaaacgacg ctatccgcca gattgcggtc
    10021 accaccaatg gttaccgtct ggaacgcgat gtggcgaact ggcgcgatgc gggacttact
    10081 ggcattaacg tcagtgtcga cagtttggac gcccgccagt ttcacgctat taccgggcag
    10141 gataaattca accaggtcat ggcagggatt gatgctgcat ttgaggccgg ttttgagaag
    10201 gtcaaagtca ataccgtgct gatgcgtgat gttaatcatc accagctcga cacctttctg
    10261 aactggatcc agcatcgccc tatccagctg cgtttcatcg aactgatgga aacgggcgag
    10321 ggcagcgagc tcttccgtaa gcatcacatc tctggtcagg ttctgcgtga cgagctactg
    10381 cgtcgcggct ggatccacca attacgtcaa cgcagcgacg gtcccgcgca agtcttttgc
    10441 catccagatt acgccggaga gattggcctt atcatgccgt atgaaaaaga cttctgcgcc
    10501 acttgcaacc gcctgcgcgt ttcctccatt ggtaaactcc atctctgcct gtttggtgaa
    10561 ggcggcgtta acctgcgcga tctgctggaa ggcgataccc agcaacaggc gctggaagcg
    10621 cgtatttcag cggcgctgcg ggagaagaaa cagacccatt tcctgcatca aaacaacacc
    10681 ggtattacgc aaaacttatc gtacattggc ggctaaaacg tcaaaaggag agatcagatg
    10741 agtcaggtaa gcactgaatt tatcccgacc cgtattgcta ttcttacggt ttctaatcgt
    10801 cgcggtgaag aagacgatac ctccggtcac tatctgcgcg attcggcgca agaagcgggc
    10861 catcacgttg tcgatgaagc cattgtgaaa gaaaaccgct acgctattcg cgctcaggta
    10921 tctgcgtgga tcgccagcga cgatgtacaa gtggtattga ttacgggtgg tactggcctg
    10981 acggaaggtg atcaggctcc cgaagcgctg ctgccgttgt tcgaccgtga ggttgaaggt
    11041 tttggtgaag tgttccgtat gttgtcgttt gaagagattg gcacttccac gttgcaatct
    11101 cgtgcggtag cgggcgtcgc taacaaaacg ctgattttcg ccatgccggg ttcgaccaaa
    11161 gcgtgccgta ccgcatggga aaatatcatc gcgccgcagc tggatgcccg tacgcgtccg
    11221 tgtaatttcc atccacattt gaagaaataa gtatgtcgca actgacccat atcaacgccg
    11281 ctggcgaagc gcacatggtg gatgtctccg ccaaagcgga aaccgtgcgt gaagcgcggg
    11341 cggaagcctt tgtcaccatg cgcagcgaga cgctggcgat gattattgat ggtcgccacc
    11401 acaaaggcga cgtatttgcc actgcgcgta ttgccggtat tcaggcggca aaacgcactt
    11461 gggatctgat cccgctgtgt cacccgctga tgctcagcaa agttgaagtc aatttacagg
    11521 ccgagccgga gcacaatcgg gtgcgtatag aaaccttatg ccgcctgacc gggaaaaccg
    11581 gtgtcgaaat ggaagcgtta accgcggcct ccgtggcggc gctgaccatt tatgacatgt
    11641 gcaaagcggt gcaaaaagat atggtgattg gtccggtacg tttgctggcg aagagcggcg
    11701 gcaagtcggg tgactttaag gtggaagcgg atgattaaag ttcttttttt cgcccaggtg
    11761 cgcgagttgg tgggaacaga tgcaaccgaa gtggctgcgg attacccaac cgttgaagcg
    11821 ttacgccagc acctggctgc gcagagcgat cgctgggcgc tggcgctgga agatggcaaa
    11881 ttgctggctg ccgtcaacca gacgctggtg agttttgacc atccgctgac tgacggcgac
    11941 gaagtagctt tcttcccgcc ggtaaccgga ggttaagatg gcagaaacca aaattgttgt
    12001 cggcccgcag ccgttcagcg taggagaaga gtacccgtgg ctggcggagc gtgacgaaga
    12061 cggtgcggta gtcaccttca ccggaaaggt gcgcaaccat aacctgggcg acagcgtcaa
    12121 agcattaacc ctcgaacact atccggggat gactgaaaaa gcactggcag aaattgttga
    12181 tgaagcgcgt aaccgctggc cgctggggcg cgtcacggtg attcaccgca tcggggaatt
    12241 atggccgggc gatgaaatcg tttttgtcgg tgtcaccagt gcgcatcgca gcagtgcgtt
    12301 tgaagccggg cagtttatta tggattatct caaaacccgc gcaccgttct ggaagcgcga
    12361 agccacgccg gaaggcgacc gctgggttga agcccgggag agcgatctgc aggcggcaaa
    12421 acgctggtag ttttttgtta gccggataag gcaccgctgc gtccggcaat ttcaccgctc
    12481 agtacatgcg tttacgctgc gtcgatgcac agcctcatca ttttgcagta tccttaagat
    12541 attccttata tcttcaggag atcgtcatgg acagattccc acgttctgat tcaatcgtac
    12601 aaccctgggc tggcttgcaa acttatatgg cgcaagtcta tggctggatg accgttggct
    12661 tgttgctgac cgcatttgtt gcctggtatg cggctaattc cgcggccgtg atggagctgt
    12721 tgttcactaa ccgtgtcttt ttaatcggtc tgatcatcgc gcaattagca ttggttattg
    12781 tgttatcagc gatgattcaa aagctgagcg caggtgtaac gacgatgctc tttatgcttt
    12841 attcggcgct gacgggtctt acgctttcca gtatattcat tgtctatacc gctgcttcta
    12901 tcgccagtac tttcgtcgtt actgccggga tgttcggcgc aatgagcctg tacggttaca
    12961 ccacgaagcg cgatttaagt ggctttggca atatgctgtt tatggcgtta atcggcattg
    13021 tgctggcatc gctggtcaac ttctggctga aaagcgaagc attgatgtgg gcagttacct
    13081 acatcggcgt gattgtcttt gtcggattga cggcgtatga cactcagaaa ttgaaaaata
    13141 tgggtgagca gattgatacc cgcgacacgt cgaacctgcg caaatattcc attcttggtg
    13201 cgttaacctt gtatctggac ttcatcaacc tgttcctgat gttgttacgg atcttcggca
    13261 accgccgtta attctttttc accatcacta tcccggccat gtgtcgggat ttttttctta
    13321 ctaaaaacga cacttaacaa gtaccaggta taaaatatgc cttatgagcg agcggaatcg
    13381 ttttccgtat ccataaatac aaaagtattt tgtatgcgtg gttttaaatt tattgatggt
    13441 gaatattaat gttttttcta aggacaacta aaagaatgga atcatactcg caaaacagca
    13501 ataaattaga ttttcagcac gaggccagga tattaaacgg tatatggctc attaccgctt
    13561 taggtttggt ggcaaccgca ggactagcct ggggagctaa gtatctcgaa attacggcaa
    13621 ccaaatatga ttcaccacca atgtatgtcg ccatagggtt attattactt tgtatgtatg
    13681 gcttaagtaa ggatatcaac aagataaatg ccgccatcgc gggcgtaata tatctgtttt
    13741 tactctcttt ggtggcgatt gtcgttgcaa gtttagttcc tgtatatgcc attatcatcg
    13801 tgttcagcac tgcgggcgcg atgtttttaa tcagtatgct ggccggttta ttatttaatg
    13861 ttgatcctgg ttctcaccgt tttatcatta tgatgacgtt gacagggttg gccctggtaa
    13921 tcatcgtgaa tgcggcatta atgagtgaac ggcccatttg ggtaataagt tgcttaatga
    13981 ttgtgttatg gccaggcatt atctcgcatg gacgaaataa gctccttgaa ctggcaggaa
    14041 aatgccatag tgaagagttg tggagtccgg ttcgttgcgc ttttacaggt gcattaacac
    14101 tctattacta ttttatcggc ttctttggga tacttgccgc gatagctata acgcttgtct
    14161 ggcaaaggca tacgcgtttt tttcattagt cgtaaatgca gatactaaag aacttaacta
    14221 aacttcacat cgccgcttca tttttcgccc gtagcttctt cgcttggctt tccagtagca
    14281 ggtaacagat cagcgccagc agcagcggga taaagtaata caggacgcga taagcgagca
    14341 gggcggcgat aatgctccct ttcgaggtat gctccccagc cagtagtgcg ataaacaccg
    14401 cttccagcac accgatcccc gccggaatat gcacgatgac gccagcaata ctactaacca
    14461 gtaacacgcc cagtacaaag aaatagttca cgctttgacc aagtaacagc cagataatcg
    14521 cccccattac catccagtta acgctggaaa tcagcatctg agcgagggcg aatttccatg
    14581 aaggcagcac cagcttttgc cctttgatgg tcatatggcg gtgcttcgcg aaagcgcaaa
    14641 accacaaata aaccgcgata atcatcagta agccaatgcc gagaatacgc agcgtagttt
    14701 gatcgacata ccagtaatcc ggcaactcca ccacgcctgc ggtaaagata atccctgcca
    14761 gtaaaatata gcccagccag ttggtggtaa tactgagcga ggaaatccgc gtaatagtgc
    14821 tgcccggtaa ccctagtcgg gagtacaaac gatagcgcat accaatgccg ccgacccagg
    14881 tactgagcgt caggttgaag gcgtagcaga taaacgacac cagcatcacc tggcgcttcg
    14941 ccagtttgtg accgcagtaa aagcgggcga gcaggtcata gcagccgtaa atcagatagc
    15001 tgacgaccac cagcccgacc gcactaagca gcgcaacgcg attgtagtcg cggatgacct
    15061 tccagacctc ttcccagtcc acttttttgg cgtagaccac cagcaacaca atcaccgcga
    15121 taaaaaacag ccaggtgagg atcttctttg ctaagcgcca gcgcgggtgt gatttactca
    15181 tcaggggttt acccccgtgt tttcagtttc tacccgatcc tgcgtttcca ttgtcggttg
    15241 tgcgggggga tcaacctgcg tcagacgtgg tgtgtgtgcc ggaagccagc caaccagcgc
    15301 cgggaagtgg cgtaaaaagt ggaacgccag cacgcttttg gtcaggttcc accaggtgcg
    15361 tttgggcagc atggtttcat ccacctgctg acaatctgcg gcaataatgc cgttcagatt
    15421 atcgcgcagc gtctggttaa aatgacgatc gtggatgatg acatttgctt cgagattcag
    15481 tgacaaactg agcggatcga gattactgga ccctactgtc gcccagtgat cgtccatcaa
    15541 tgccactttg ccgtggagcg ggcggcggcg gtactcaaaa acctgaacgc cgcctttaac
    15601 cagatagtta tacagcaagc gcgcaccgac tctgacaatc ggcatatccg gttcgccctg
    15661 aatgatcagt ttgatccgca ccccgcgccg tgccgcttta cgcaaggcgt gtaaaaatcg
    15721 atagccgggg aagaagtagg cgttggcgat aatcacttcc cgctgcgcct gagtgagcat
    15781 tttcagataa tggcgttcaa tatcatcgcg atgttcttcg ttatcgcgcc agaccagcaa
    15841 tacctgcgct tctcccggct ggcggttctc ttccgctttg tgatgacgtc gccaccagcg
    15901 tcgtgccgcg ctctgtccag gcaggttttc cagctcaaac tggagaatat cttcgacaat
    15961 cggcccttca aggcgtaccg cgtaatcctg tttagcctct ggaccgtagc tggacatatg
    16021 ctcggcggag taattcagcc cgccaataaa ggcgatacgc gcgtcgatca ccacaatttt
    16081 gcgatgcatc cggcgaaaca cattggtgcg cataccaaaa aggcgagggc ggggatcgta
    16141 gtagcggaac actacgccag ctgccgtcag ttcattgaca aactcatcgc tgagatccgg
    16201 cgaaccgtag ccatccagca agacttccgc tttaaccccg cgttgcgctg ctgccagtag
    16261 tgccgcatgc agctgtttgc cgacgttatc ctcaaaccag ataaacgttt caagaatgat
    16321 gcgttcttgt gcctcgccaa tcgccttaaa caccgcggga taatattgct cgccgttttc
    16381 cagcaactgg atcttattgc cttcgcgcca gctacatttc ataaatgaat ctccgcactt
    16441 aaaggggcat gatcagaaag gtgtcgccat gtccgcagcg gcaacgcggt tggcgcgctg
    16501 gcgctggcat ttttgacgta gatcctgtcc agtcgtagta gaggaaattg caccggaaac
    16561 gtgcgcgccg ggcgtccgtg ggcgcgggta aaaatctcat ccagtccggc ctgcactttt
    16621 aacggatgat tagctttttg ccgccagtca ttgaaatcac ccgccaccaa taccggttcg
    16681 ccgtccggta gctcattcac ccattcggcg agcatcgcaa gctgcgcctg acggtgcgcc
    16741 tcacgcaggc ccagatgtac gcacatcaca tgaatcgctt ttccggtcat cggcggcaca
    16801 atgcggcagt agagcacgcc gcgcttttcc gcaccatcga ccgaaacatc gcgattctca
    16861 taatgttcaa tgggataacg cgacagtacg gcgttgccgt gatgcccttc cgggtatacg
    16921 gcattgcgac cgtaggcaaa atcgctccac atagtgtcgg cgagaaactc gtagtgcgag
    16981 gtatcgggcc agttttccac atgcagcgga tgaacttcgt gcgcgcccat cacttcctgc
    17041 aggcaaacaa tatcggcgct gacggtacgc acggcatcgc gaagttccgg caaaatgaag
    17101 cgtcggttaa acgcggtaaa gcctttgtga atgttgatgg tgagcacctt gaacgaaaat
    17161 tgttgtgttt gatcgggcat aattttcctg tctttgcctc ttatctcatt gaaatagtgt
    17221 agtcggcgtc acaaaaaggt gcggtcttac ggaattttcc gtaaagttcg gtactctgag
    17281 taagtagaga taaattcttc aggagagaag ccatgaagtg gcaacaacgt gttcgtgtcg
    17341 caacgggtct aagttgctgg cagattatgt tgcatttact ggtagtggcg ctgctggtgg
    17401 tgggctggat gagtaagact ctggttcacg tcggcgtggg attatgcgca ctgtattgtg
    17461 tcacggtagt gatgatgctg gtgtttcagc gccaccccga gcaacgctgg cgtgaggtgg
    17521 cagacgtgct ggaagagctg accacgacct ggtattttgg cgcagcgctg attgtgctgt
    17581 ggctgttgtc ccgcgtactg gaaaacaact ttttgctggc aattgcaggg ctggcgatcc
    17641 ttgctggccc ggcggtagtg tctttgctgg cgaaagataa gaagttacat caccttacgt
    17701 ctaaacatcg cgtacgccgc tgaccctgtc gtggccgtta tcaccagtag cggccacaaa
    17761 ctattccaca caatatccag actcgcatcc ttcaaataaa tctgcttggt aatgtccgta
    17821 aagtggcgaa tagggttaat ccacgtcaga ttttgcagcc acactggcat gttttccacc
    17881 ggcgaaacgt aaccggaaag gagaatggcg ggcatcataa aaacaaacac gccgataaac
    17941 gcctgctgtt gtgtcgagca gagtgatgaa atcaacagac cgaatcccac cagcgataaa
    18001 ccgtaaatca ccatcgtaaa gtagaacagc gctagcgatc cggcgaaggg gatttgatac
    18061 gcccagatac caatcgccag cacaatggtg gcctggaagg tggcgacaat taacgccggt
    18121 acggctttgc cgatgaagat ctgccaggtg gtgagcggcg aaaccagtag ctgatcgagc
    18181 gtaccttgtt cacgttcgcg ggcgacggaa agtgaagtga cgatcattac gccgatagtg
    18241 gtgatcatgg cgatcagcga cggcaccaca aaccatttgt agtcgagatt cgggttatac
    18301 cagttgcgta ccaccagctc gctgttgttg ggtttcggtt ttccttccag cagctcctgc
    18361 tgataatttt tgacgatctg ttgcaggtag ttggcggcaa tttgcgcact gttggagtta
    18421 cgcccgtcga ggatcaactg caacggcgcg gtctggaagg tatccagttt gcgcgagaag
    18481 tcagccggga aaagcaccag tagtaacgcc ttttgtgtgt cgatggtcgg gcggatctcc
    18541 tgtgggcttt tcagcagcag cacatgtgta aaggcgctgg cgcgggcaaa acgttgagta
    18601 agttccaccg aatgctcgcc gttatcttca tcgtagatgg cgatggtggc gttagtcact
    18661 tccagcgtgg cggcgaacgg gaacaggatc acctgaatta gcacgggtaa aatcagaatc
    18721 gcgcgggttt gtggttcgcg cagcaacgac tgcaactctt tgcggattaa cgtccataag
    18781 cgatgaaaca tgctcttctc cctaatccag ccgacgtttg gttttcagcc acgtcaggcc
    18841 gataaacatc accgccgaag cgatcaaaaa cagcacgttt accaccagca ccactggaat
    18901 attcccggcg aggaacaggc tttgcagggt gctgacgaaa taacgagcgg gaataatgta
    18961 cgtcaccgcg cggatcaccg cgggcatact gtcgatctga aaaataaagc cggaaagcat
    19021 aatcgacggc agaaaagcgg cgttcagggc gacctgagcg gcattgaact ggttgcgggt
    19081 aatcgtggaa atcagcagcc ccattcccag ggtactgagt aaaaacaggc tggagataaa
    19141 aaacagaatc agcagcgaac cgcgatacgg cacgccgaga ataaacactg acaccagcat
    19201 acacagcaac atcgccagca tcccgagaaa gtaataaggg atcagcttac acagcagcag
    19261 ttccgtgcgg gtaatctccg tagagagcag agcctccatg gtgccgcgtt cccattctcg
    19321 cgccaccacc agcgaggtga gaatcgcgcc gatgaccgtc atgataatgg tcaccgcacc
    19381 cgggataata aagtgctggc ttatcgccgc cgggttaaac cagtagcggg tttgtacatc
    19441 aataagcggt tcaaaagtct gcccgttgtc ctccgctcgc tgcatttgcc agatctgcca
    19501 gatcccttcg acatacccct gcacaaagtt agcggtattc ggctcactgc cgtcggtgat
    19561 cacctgaatc ggtgcggtgg cgttggcgcg ctccatctgt tcggcgaaat ccacgggaat
    19621 aaccaccaga ccgcgaattt tccccgactg cattttggcg atcagttcct gacggttatc
    19681 gctgatggtg gcgtcgatgt agggcgaacc ggtcatggtg tgggtgaaat ccagcgccgc
    19741 ttcgctacgc tgttccagca aaatcccgac ccgcagcttg ctggagtcga ggttaatgcc
    19801 gtaaccaaaa ataaacagca gtagcagtgg gatcactacc gcaatcagcc agctactcgg
    19861 atcgcgaacg atctgccgcg tctctttgac gcacagcgcc cgcacgcgat gccaggaaag
    19921 gatcgggtta ctcattgcta tgctccttat cccagtcgtg gatcaactga ataaaggctt
    19981 gctccatggt gggatcgggt tgctcatcgt tagctgactg tgctttcaaa tcgtccggcg
    20041 taccgctggc gattaatttc ccacggtaca ccaggccgat gcggtcgcaa tattccgctt
    20101 catccataaa gtgggtggtg accatcaccg tgacgccttt ctctaccatg ctgttgatgt
    20161 gcagccaaaa ttcacggcgg gtgagggggt caacgccgga agtcggttcg tcgagaaaga
    20221 gaatatccgg ttcatgcatc agcgaacagg ccagcgccag ccgctgttta aaacctaatg
    20281 gcagttcatc ggtggcgtgt gaggcaatac ttttcaggcc aaatgcctcg ctcatacggg
    20341 agattttttc gttctgcgcc cgaccgcgta agccatacac accagagaaa aagcgtaaat
    20401 tctgttcgac cgtcaggttg ccgtagagcg aaaatttttg cgccatatag ccgagatgct
    20461 ggcgcgcttt accggaactc tctttcagat ccatccccag caccagcgcc tggccggaag
    20521 tcggcaccag caaaccgcac atcatcttaa aggtggtcga tttacccgcg ccgtttggcc
    20581 ccagcaaacc aaaaatctcc ccacgtttaa cggcaaagtt gacgtgatcg gtggcggcaa
    20641 aatcaccaaa tttcttggtc agttctttcg cttcgatcac cgtctcgccg ggtgtgcctt
    20701 ctaccgtatg caatattgca cccagcggcg attctgaggt tccggcaccg cccagcaaat
    20761 caataaacgc atcttcaaaa cgcggcgtag tttcgttgat gttgatttcc ggcatcccgt
    20821 cggcatggcg aatatcgtct ggtgtggcct cttttttgag gatcagacgt accgatttcc
    20881 cctgaatcat gccatcgctg acctgcggca gtttcaaggc gcgttgcaac agtttgcggt
    20941 tgccctcgtg tggactggtc atcagaaagc tgcgtccggc catggtttgt gtcagggctt
    21001 ttggttctcc ctgatacagc aactcgcctt cgttcatcag taacacgtca cggcactgct
    21061 cggcttcgtc gagatacgag gtactccaga ggattaacat cccttcgccc gccagctcat
    21121 gcaccatctg ccacagttcg cgccgtgaga tagggtcaac gccgacgccg ggttcatcga
    21181 gcagcaacac tttcggttcg cccaccaggg tacaggccag gccgagtttt tgtttcatcc
    21241 caccggagag cttgcccgcc aggcgtccgg taaacggccc aagagacgta aactccagca
    21301 ggcgagcaaa agtttgctta cgtgcctcgc cggtgacgct gcgcaaatcc gcgtacagat
    21361 tgaggttctc catcaccgtg agatcttcat acagaccaaa tttctgcggc atatagccga
    21421 gcacggcgtg cagcgcgccg tcgtttttga tcggatcaaa gccaatcacc gtggcactgc
    21481 cgctgtcggg tttcagtaat cccgccaaca tccgcatcag tgtggtttta cctgcaccgt
    21541 ccggccccac caaccccgtc acataaccgg cgtgaatggt acaatcgagc ggcgcgacgg
    21601 cgggcttgtc catgcccgga aagcgttttt ccaggccgtt cagcgtgata acggcatcat
    21661 tcatgtcctg cctcgtcacc gaattgtacc gtcactggca ttccctggcg taacgcatca
    21721 tcggcgtcgg tcaccacaat acgcaggcga tagacgaggt cggtacgcag atccggtgtt
    21781 tcgacggttt tcggggtaaa ttcagcagtc ggcgaaacga aaccaatctg cccgtgatac
    21841 ggcttgtccg ggcgaccatc ggtataaagc agcactttgc gccccggctg ggcctggtca
    21901 agattccgtt catcaacata agcgcgcacc cacaccggac gcgttagtga aacggtaaac
    21961 accgtgccac cttcattgag aaccgtgcct ggctctaccg cgcgcgttaa cagcgtgcca
    22021 tcagacggtg ctatcaacgt tgagtcctgt aaattcagct ccgcctgcgc cagttgcgcc
    22081 tgcgcctgtt cgaggctggc tttcgcctga gcgatgtcct gttcacggtt accggaacgg
    22141 tactgacgca atttatcctg tgctgatttc agcgttgcct gtgcctggtc gcgcgaggag
    22201 cgggcatttt ccaggtcatt tgccgaaatg gtgcggcttt tccacagtcc ttgctggcgg
    22261 ttatagaagt tctgcgcata gtcataggcg gcttgcgcct gtttcaccgc tgcggcggcc
    22321 tgagcgattt cttcatcgcg atacccggca agcatcagat catactgcgc ctgtgccacc
    22381 gagacgcccg ctttcgcctg catcagggca atctcatacg gcttgtgatc cagttcgccc
    22441 agcacctggc ccgctttgat agcatcacct tcgtccaccg ccagcgattc aacgcgcccc
    22501 ccaacacgga aactaagatt caccgtacga atatccacgt tgccatacag cgtcaggccg
    22561 ttatcctggc ggctttgata ccaccagtag cctccggcaa ccacggcggc aagtaccact
    22621 accgccagtc cgatcacgac aggttttttc atcactacaa actcctttgc gataatcctt
    22681 gcaaaatcag gtcgatatga caggtcaccg tctggttgat cagttcggtc ttttcttcat
    22741 cgaacgcggt ccagccggta cgtaacagaa ttgtttcttt gccaagacgg aacgccagaa
    22801 tctcaccaat caacgcatgg gtatgaagga tcatgcgggt gtcattagcg tcgcagccgg
    22861 tccaggcggc aatcaggcgt gtcaggtggc tgtgtagcgg actaatcacc tgttcgtgca
    22921 ccaggtggta ggctgccgtg ggagagagct gctcacggga gataaacttg ctgaggttga
    22981 cggtgtcatc ctaggtgagc agcttaatca tgttcctgca ggcgcgaagg atcagttcac
    23041 ggatggcagc ccgatcaggc tgtggttggg cgaacaagcg ttcggcttcc tcggcatgcg
    23101 gacggaactg ctcgccaata aaatcggcaa tccactgggc gcaggcgagg tacaaatctt
    23161 ctttcgaacc gaagtagtag gtgatggcag cgatattctg cccggcctgg gcggctatct
    23221 cgcgggtggt ggcgttcatt ccatattcac caaactgcgc cagtgcggca gcaatcagct
    23281 gttttttcgc ctgttcaccc ttgattgtca tggcaggatt attcatcgca cagtccattc
    23341 ttaatcaaat gattgattaa gattatgact ccatatggga gttgtccagt atggctaaga
    23401 attttagcta cgccagtcac agggataatt tatgcgctgc gtcacaaaaa ctgctacact
    23461 ccgctccctc atgacattgt ggtttttgtc attttccttt tcagtatctc cctgaaaact
    23521 acaccggtaa cggtcggggc ggttcggagt agttatgtct ttcgattctt tgggtttaag
    23581 ccctgatatc ctgcgcgccg ttgccgagca gggttaccgt gaacccaccc ctattcagca
    23641 gcaggcgatc cctgctgtgc tggaaggccg cgacctgatg gctagcgccc agaccggcac
    23701 cggcaaaaca gcgggcttta cgctgccgct gttgcaacac ctgatcactc gccagccgca
    23761 cgccaaaggg cgtcgtccgg tacgtgcgct cattcttacc ccgacccgtg aactggcggc
    23821 acagattggc gaaaacgtcc gtgattacag caaatacctg aacattcgtt cgctggtggt
    23881 gtttggcggt gtcagcatta acccacaaat gatgaagttg cgcggcggcg ttgatgtgct
    23941 ggtggcaacg ccgggacgtt tgctggacct ggaacatcaa aatgcagtga agctggatca
    24001 ggttgaaatc ctcgtcctcg atgaagctga ccgcatgctc gacatgggct ttatccacga
    24061 tatccgtcgc gtgttaacaa aactgccagc gaaacgccag aacctgctgt tctctgcgac
    24121 cttctctgac gatattaaag ccctggcgga aaaactgttg cacaacccgc tggaaatcga
    24181 agtggcacgc cgcaataccg cgtctgatca ggtgacccag cacgttcact ttgtcgataa
    24241 gaaacgcaaa cgcgaattgc tgtcgcacat gattggtaaa gggaactggc agcaggtgct
    24301 ggtgtttacc cgtaccaaac acggcgctaa ccatctggct gagcagctca ataaagatgg
    24361 catccgtagt gtggcgatcc acgggaataa atcgcaaggt gcgcgtaccc gtgcgctggc
    24421 tgattttaaa tcgggtgaca ttcgcgtact ggtggcgacc gatatcgctg cgcgcggtct
    24481 ggatatcgaa gagttgccgc acgtcgtcaa ctatgaactg ccaaacgtac cggaagatta
    24541 tgtccaccgt atcgggcgta ccggtcgtgc ggctgctacc ggtgaagcgt tgtcgctggt
    24601 gtgtgttgat gaacacaaac tgctgcgtga tatcgaaaaa ctgctgaaaa aagagatccc
    24661 gcgcattgcg attccgggct atgagccgga cccgtcaatc aaagccgaac cgatccagaa
    24721 cggtcgccag caacgtggcg gcatcaggct gatgaacaaa cgcaaaactg cctgatgcgc
    24781 tacgcttatc aggcctacgt gaactctgca atatattgaa tttgcatgct tttgtaggcc
    24841 ggataaggcg tttacgccgc atccggcatt tcacaacaag cacttgtcag caatttgaga
    24901 acacgggaaa aaatttatct gttttaccgc ccataagcca ccgagacgtt acttctctat
    24961 agccaattgt tcgcgcaact ccattaaaag gtagcccagt cgattcttgc ccttaccatt
    25021 accaccgtct ccccagtaag catcgttttc cgtatgctca accagttttg cgggcgcggt
    25081 tgccagcaag agcacacgca gttctgcatg ctgttcgaat ttagcgcgaa gtgctttacg
    25141 catcacctgt tctttaaccg actcccagtt tttacgaagt ggcttggaac gattacgccc
    25201 catgcgagcg gcgaccatgg gggaagaaac ccgacggatc tcttcgcggt atttttcatc
    25261 aaggaatttt tgtgcctgaa aatagtgttc ggaggtgggc caggtattcc cgtcaacctt
    25321 gatgggcctg gcggcaaagt tagaaaaatc accgtagtca tcgctggtgc tgtagaaatt
    25381 tatgatggtg tcttgcatga cgtgttggat cctttgtgct cgaacgggca ttaaaccgca
    25441 ttatgttggt ggttattgcg agccgctttc cagaaacaga aaaaccatta cccctgaaaa
    25501 ccgaaaaatg ccacaatatt ggctgtttat acagtatttc aggttttctc atggcattaa
    25561 ccgccgcgct taaagcgcaa attgccgcct ggtataaggc gcttcaggaa cagatccccg
    25621 actttattcc ccgtgcgccg cagcggcaga tgattgcgga cgtcgccaaa acgctggccg
    25681 gagaagaagg gcggcatctg gcgattgaag cccccaccgg cgttgggaaa acgctctcct
    25741 atttgatccc cggcatcgcc attgcccgcg aagagcaaaa aacgctggtg gtgagtaccg
    25801 ccaacgtggc gttgcaggat cagatttaca gcaaagattt accgctgctg aaaaagatca
    25861 ttcccgatct taaattcact gccgctttcg ggcgtgggcg ctacgtttgt ccacgtaatc
    25921 tgacggcgct cgccagcact gaacccacgc aacaggatct gctggcgttt cttgacgacg
    25981 aactgacgcc aaataatcag gaagagcaaa aacgttgtgc gaagctgaag ggcgatctcg
    26041 acacttataa atgggatggt ctgcgcgatc atacggatat cgctatagat gatgatctct
    26101 ggcgtcgttt gagtaccgac aaagccagct gcctcaaccg taactgttac tactatcgtg
    26161 aatgcccgtt ttttgttact cgtcgggaga ttcaggaagc ggaagtggtg gtggcaaacc
    26221 atgcgctggt gatggcggcg atggaaagcg aagccgtact gcctgacccg aaaaatctgc
    26281 tgctggtgct ggacgaaggt catcacctgc cagatgtggc ccgggatgcg ctggagatga
    26341 gcgctgaaat caccgtgccc tggtatcgac tacagctgga cttgttcacg aaactggtcg
    26401 ctacctgcat ggagcagttt cgcccgaaga ccatcccacc gctggcgatc cctgaacgtt
    26461 tgaatgcgca ttgtgaagag ttgtatgagc ttatcgcctc gttaaacaac attctcaatc
    26521 tctacatgcc tgccgggcag gaggcagagc accgttttgc gatgggcgaa ctgcctgatg
    26581 aattgctgga gatctgccag cggctggcaa aactcaccga gatgctgcgt ggtctggcgg
    26641 agttatttct taacgattta agtgagaaaa ccggcagcca cgatattgtg cgtctgcatc
    26701 ggttgatttt acagatgaac cgcgcgctgg ggatgtttga ggtgcaaagc aaactctggc
    26761 ggctggcgtc tctggcgcaa tcttccggtg cgccggtgac caaatgggcg acgcgggaag
    26821 agcgcgaagg gcagctacac ctctggtttc actgcgtggg aatacgtgtc agcgatcagc
    26881 tggaaaggct gctgtggcgc agtattccgc acattattgt cacctccgca accttgcgtt
    26941 cgctgaatag tttttcgcgt ttgcaggaga tgagtggtct gaaagagaaa gcgggcgacc
    27001 gttttgtggc gctggattcc ccctttaacc actgcgaaca gggcaaaatt gttattcccc
    27061 ggatgcgctt tgagccttcc atcgacaacg aagagcaaca cattgccgaa atggcggcct
    27121 ttttccgtga acaggtggag agcaaaaaat atctcggtat gttggtactg tttgccagcg
    27181 gacgggcgat gcagcgcttt ctcgactatg tgacggattt acgcctgatg ttgctggtgc
    27241 agggcgatca gccgcgttat cgcttagttg aactgcaccg caaacgcgtt gccaacggtg
    27301 agcgtagcgt gctggtgggc ttacagtcat ttgccgaagg gcttgatttg aaaggtgatc
    27361 tgctcagcca ggtgcatatc cacaaaatcg cttttccgcc catcgacagc ccggtggtga
    27421 tcaccgaagg ggaatggctg aaaagcctca accgctatcc gtttgaggtg caaagcctgc
    27481 cgagcgcctc gtttaacctg attcagcagg ttgggcgact gattcgaagc cacggttgct
    27541 ggggcgaagt ggttatctac gacaaacgct tgctgacaaa aaattatggc aagcgactac
    27601 tggatgcatt accggtattt ccgatagagc aaccggaagt ccctgaaggt atagttaaaa
    27661 agaaagaaaa aacgaaatcc ccacgccgtc ggcggcgtta atgatgtgag tcaggtaagg
    27721 agtcgtaaat ggactatcgc aaaatcatta aagagatcgg gcgcgggaaa aaccacgcgc
    27781 gcgatttaga ccgggatact gcgcgcggtc tgtatgctca tatgctcaat ggtgaagtcc
    27841 ctgaccttga gttgggcggc gtattgattg cgctgcgtat caaaggggaa ggggaagcag
    27901 agatgctcgg cttttacgaa gccatgcaaa atcacaccat caagctgacg ccgcctgcag
    27961 gcaagccgat gccgattgtc atccccagtt acaacggcgc gcgtaaacag gctaacctga
    28021 cgccattatt ggcgattctg ctgcataaac tcggttttcc tgtggtggtt cacggagtta
    28081 gcgaagatcc aactcgcgtg ctgacagaaa ccattttcga actgatgggt attacaccaa
    28141 cgttgcacgg cgggcaggcg caggcgaagc tcgacgaaca tcaaccggtg tttatgcctg
    28201 tcggcgcgtt ttgcccgccg ctggaaaaac aactggcgat gcgctggcgg atgggcgtgc
    28261 gtaacagtgc acataccctg gcgaaactgg cgacgccatt tgccgaaggt gaggcactgt
    28321 gtctttccag cgtttcgcat ccggaataca ttggacgcgt cgcgaagttc tttagcgata
    28381 tcggcgggtg ggcgttacta atgcatggta ctgaaggcga agtgtatgct aatccgcagc
    28441 gctgcccgca gatcaatctc attgaccgtg aagggatgcg ggtgctgtat gaaaaacagg
    28501 accctgctgg tagcgagtta ctgccacaag caaaagatcc ggaaaccacg gcgcagtgga
    28561 ctgagcgttg cctggctggc agcgaagcga ttcccgaatc gctgaaaatc cagatggctt
    28621 gctgcctggt ggctacgggt gaagcggcaa ctatcagcga tggcctggcg cgcgttaatc
    28681 aggcatttta atttttatct cagccggatg agccacgtca tccggctttt accccaccgc
    28741 aatatgaaat tcctgcatct ttattgacct tcccgcgccc ggcgtgcagc ataaaaaaac
    28801 aacaaacaca taacataaac aggagttaac catggaaagt ggtcatcgct ttgatgctca
    28861 gacgctgcac agttttattc aggctgtatt tcgtcagatg ggtagcgagg aacaagaagc
    28921 gaaattagtt gccgatcatt taatcgcggc aaacctggca gggcatgatt ctcatggtat
    28981 tggcatgatc ccaagctatg tgcgttcctg gagtcagggg catctgcaaa ttaaccatca
    29041 tgccaaaatc gttaaagagg cgggggcggc agtcacgctc gatggcgatc gcgcattcgg
    29101 tcaggtcgcg gcacacgaag cgatggcgct ggggattgag aaagcgcatc agcacggtat
    29161 tgctgccgtg gcgctacata actcgcatca tatcggccgt atcggttact gggcggagca
    29221 gtgttcagcg gcggggtttg tctctatcca ctttgttagc gtggtcggta ttccaatggt
    29281 cgcgccgttc cacggtcgcg acagccgctt tggcaccaat cccttctgtg tggttttccc
    29341 tcgtaaagat aatttcccgc tgttgcttga ttacgccacc agcgccattg catttggcaa
    29401 aacccgcgtc gcctggcata aaggcgtccc cgtgccgcca ggttgcctga ttgacgttaa
    29461 cggcgtgccg acgaccaatc cggcggtaat gcaggagtcg ccgttggggt cgctgttgac
    29521 ctttgccgaa cataaaggct acgcccttgc agcgatgtgt gaaattcttg gcggggcgct
    29581 ttctggcggt aaaacgacgc atcaggaaac gttgcaaacc agccccgatg ccattcttaa
    29641 ctgcatgacc actatcatca tcaacccgga actgttcggc gcgccggatt gtaacgcgca
    29701 gaccgaagcc tttgccgagt gggtgaaagc ctcgccgcat gatgacgata agccgatttt
    29761 gctaccgggc gagtgggaag tgaacacgcg tcgcgaacgg caggagcagg ggattccatt
    29821 ggatgcggga agctggcagg ccatttgtga tgcggcgcgg cagattggta tgtcggaaga
    29881 gacgttgcag gctttctgtc agcagttagc cagctaaaaa aaagcccgtc cagtggcgga
    29941 cgggcaaaca agggtaacat aggatcaatg agggttagag catatgcgtc tgtcggcaaa
    30001 cagacaggga aatacttgtg ctggacgtag cgtaaacgcc tgatccgtcc aacggttcgg
    30061 attttgtagg cctgataaga cgcggtagcg tcgcatcagg catctgttgc cggatgcggc
    30121 ataaacgcct tatccgtcct acgaattact tataaattac cgcagtaccg cttaatttat
    30181 tgttgttggt ggcggaagtg atgctgtatc cactggcacc ggctgccgcc gctttctcag
    30241 ccagtttcgc ttccagggca tcgagggggg atgcgccatc ggcagaaacc acgccgattt
    30301 tattcatgtt ctgtgcctgg gatgccgtta ccggttccgc cgcgaacacg ccaaatgaca
    30361 gagttgaaag agccatagca gcaacaacag tattgatagt tttcataatt aatctctcgc
    30421 aggtgatttt tgtataagtg acgttgtttc gtcgatgtga tgagtatcac gtttttttac
    30481 gagagataaa atcgaagaga attgaccgcc ttgttcaaat aaattgattg atatctaaca
    30541 tactgataaa ccatttattt gttgtactta tggacttgtt ttatgccgcg tggcagtcac
    30601 agcagcgtta aaggcacgga atgccacgca aaatacaaat tatactcgct atgcatctcg
    30661 ccagttgtag ttaacgtaac tgtctgtctt tcgaacctct gcgattatat cctgtatacg
    30721 ctggtttttg taaatctttc tcctgctgac aatgaataca taagcgcacg ccaggaatgg
    30781 cttcccgacg ggcttgcggg atgggggcac cgcactcttc acattcatac aggctttcgc
    30841 cacgcggaat ttcaccccga gcgcgggcaa tcgcatcttc aattgtactg ttgatctgtt
    30901 cgttgacggc gtcatcgtta gcccaagcgg atgccatatc gtcctcccca tatcaatgct
    30961 tgtacagtta agtgtagcta atatagggac gaacctgggc agttcaagcg tcagatctcc
    31021 gaccattccc gcagcagatt atgataaaga ttgagcagcg acaggatctc ttcattttca
    31081 ccatagcggc ttttcagcga ctgaatattt ttgtccagtt caaacagcat ggcgcgcttt
    31141 ttatcatcgc ggatcatcga ctggatccac ataaatgatg ccactcgtac gccgcgggtt
    31201 acgggtgtca cgcaatgcag gctgctggag ggatacaaca ccagatcacc tgccgggagt
    31261 tttacccgat gttgtccaaa ggtgtcatta acgaccagtt cgccgccgtc gtagctttgt
    31321 ggatcgctta aaaacagcgt ggcagaaagg tcagtgcgca tccagccgtt ttgtggatgg
    31381 ctgcgtactg cgccatccac atggaaacca taggtttcat tgttctgata gcgattaaac
    31441 agcggcgtgg aaagggtacg cggcaaggca gcggcaaaga acaatgcatg ttggttaacc
    31501 gcgttcagca cctcattttg cagtgcggcg tataacgcgc tgcgggtgtc tacttgctga
    31561 ttgttcttaa cttgtgcacc ttgtgctccg gtggtgacgc gtccatccac ccattcggct
    31621 tgttccagtt gttcgcgaaa atgggcgacg tcctgtggcg ataacacgcc gggaatgtgg
    31681 tacatcatac aagttctcca aaaagtgggg cctgcgcccc acatctgaat cagaaatgca
    31741 tattggctgt gagcaagaag gttcttggct cgcccgggtg ataacggtag ccgctcttgt
    31801 tgattgaggc gacgtaatcg gtatcaaaca ggttgtaaac gtttagctgg aagtcgagat
    31861 tgcgattaac ccgatacccc agtttggcat cggcgaccca gtaaccttct gtaaatgctg
    31921 gcgttcccac cgcgccgtct gaacctttat gtatactgcc gatatagcgt gcgcccgcgc
    31981 caacagagat atcatcggtt gcctgatatt ggctccataa ggtgaaggcg tgctccgggg
    32041 tatacggcag cgatgaggaa ccatcctggg caacatcttt gccgtttttg atggttgctt
    32101 tttgctgggt atagccgcca atcacctgcc acgcgggagt gatattccct gccacggata
    32161 tctcatagcc ttcgacgcgt ttcttaccgt attgcgagta agtcccgtca tcattttgct
    32221 caacttcatt ttcgatatca gtacggaaca gcgcggcggt gagcaacagg cgtttatcca
    32281 gaacctgcca tttggtgcca atctcgctgg tgttggcttt ttgcggttta aaatcggtgc
    32341 ggttggcact gttaccactg ccagactgcg caagggcgaa gttgttgccg cccggaggct
    32401 gctgggaaac ggcatagtta atatagacat tgccgttttc cgtcaggtgg tacagcgccc
    32461 cggctttcca gttcaccaga ttgcccgact tggcggtgtc gacggtggtg actggagaac
    32521 cttttgccac accagctggg caggtgatgg caccgcgtcc gctgccgccg caggcggtgg
    32581 cactgtcata ttcagtatga taattatcca gacggatccc gccgttcagc tcaaaatcac
    32641 gggtgatttg cagcgtatcg aaggcgtaaa ttgcgaaggt atccgtctga ccattggcgt
    32701 ttgcgccgtt gcgcgtcagg ccgccgggat gaattctgct gtcaggatga taaatattta
    32761 ccgccggtaa cgtcaccgga ttaacgccat agttagtctg cgtttcacgg gtaaattcca
    32821 cgccggtact gacatcatga ccgatagaac cggtatagaa cgtcgaggtc aggttggtct
    32881 ggttggtgag aattttatta ctcacatctt tggtattcgc cgtgcgtgac taggtccagc
    32941 tattcacatc gctggtgggc tgagtaatat tcgacgcccc gcccataatc gccgtcatca
    33001 ggtaatcctg ctttacgcgc gaccaacggg tagtattgcg aatggtggtg ttatcgttga
    33061 tgtcgtgctc aaaacgcatg gtggcggtgt cggtggtcga atcgtcgtaa tcggaatccg
    33121 tgccgtaaaa gttatgagta tcaacttttc cggaatgatt cagggccgcc gctcccgcag
    33181 atggggcaga atagcccggc aaaccgatgg tcggaatgcc gccgtctggc gtgttgtgct
    33241 gagtgacatg cagataatta agatacaaac gatttgctgt accaaggcca aaagcgacag
    33301 aaggggcgac gccgtaacgc tcatttttga ctttgtcgcg tccggcatcg tgggtttttt
    33361 cgcccatcac attcaggcgc accgcggtag tatcaccaat gacctgattg acgtccagcg
    33421 tgccgcggcg gaaccaggcg ctgccaatac tggcggaggc gtcaatgccg gaatcattgc
    33481 gcggctgctt gctgatcata ttgatcgagc ctgtcggtgc gctgcgcccg tagtcggtgc
    33541 cggacggccc tttaatcact tcgacctgct cggtattgaa ggtgtcgcgc gagacgctgc
    33601 cgatatcgcg aataccatca atataaatac tgttagaggt atcggcacca cgcatataaa
    33661 tggcgtcgcc agtggtggag ttaccgttct cacccgcaaa aaacgcaccc acgc
//
LOCUS       DAZSIO010000026        32967 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZSIO010000026 DAZSIO010000000
VERSION     DAZSIO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32967
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.026"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..2098
                     /locus_tag="ACHX5T_001148"
     CDS             <1..2098
                     /locus_tag="ACHX5T_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR8466424.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2095..3183
                     /locus_tag="ACHX5T_001149"
     CDS             2095..3183
                     /locus_tag="ACHX5T_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR8466425.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="ACHX5T_001150"
     CDS             complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="ACHX5T_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFR8466426.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5054..6466
                     /gene="uxaC"
                     /locus_tag="ACHX5T_001151"
     CDS             5054..6466
                     /gene="uxaC"
                     /locus_tag="ACHX5T_001151"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFR8466427.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6481..7968
                     /gene="uxaA"
                     /locus_tag="ACHX5T_001152"
     CDS             6481..7968
                     /gene="uxaA"
                     /locus_tag="ACHX5T_001152"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR8466428.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8051..8602
                     /locus_tag="ACHX5T_001153"
     CDS             8051..8602
                     /locus_tag="ACHX5T_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR8466429.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="ACHX5T_001154"
     CDS             complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="ACHX5T_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR8466430.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10447..11412)
                     /gene="alx"
                     /locus_tag="ACHX5T_001155"
     CDS             complement(10447..11412)
                     /gene="alx"
                     /locus_tag="ACHX5T_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR8466431.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11683..12681)
                     /locus_tag="ACHX5T_001156"
     CDS             complement(11683..12681)
                     /locus_tag="ACHX5T_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR8466432.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12760..13452)
                     /locus_tag="ACHX5T_001157"
     CDS             complement(12760..13452)
                     /locus_tag="ACHX5T_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR8466433.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13529..14032)
                     /locus_tag="ACHX5T_001158"
     CDS             complement(13529..14032)
                     /locus_tag="ACHX5T_001158"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR8466434.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14117..15253
                     /gene="rlmG"
                     /locus_tag="ACHX5T_001159"
     CDS             14117..15253
                     /gene="rlmG"
                     /locus_tag="ACHX5T_001159"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR8466435.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15538..15852
                     /gene="higB"
                     /locus_tag="ACHX5T_001160"
     CDS             15538..15852
                     /gene="higB"
                     /locus_tag="ACHX5T_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR8466436.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15849..16265
                     /gene="higA"
                     /locus_tag="ACHX5T_001161"
     CDS             15849..16265
                     /gene="higA"
                     /locus_tag="ACHX5T_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR8466437.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="ACHX5T_001162"
     CDS             complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="ACHX5T_001162"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR8466438.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="ACHX5T_001163"
     CDS             complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="ACHX5T_001163"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR8466439.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATQEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20922..21993)
                     /gene="ygjJ"
                     /locus_tag="ACHX5T_001164"
                     /pseudo
     CDS             complement(20922..21993)
                     /gene="ygjJ"
                     /locus_tag="ACHX5T_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(22127..23560)
                     /locus_tag="ACHX5T_001165"
     CDS             complement(22127..23560)
                     /locus_tag="ACHX5T_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR8466440.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23623..24072)
                     /locus_tag="ACHX5T_001166"
     CDS             complement(23623..24072)
                     /locus_tag="ACHX5T_001166"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR8466441.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="ACHX5T_001167"
     CDS             complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="ACHX5T_001167"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFR8466442.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="ACHX5T_001168"
     CDS             complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="ACHX5T_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR8466443.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28539..28870
                     /locus_tag="ACHX5T_001169"
                     /pseudo
     CDS             28539..28870
                     /locus_tag="ACHX5T_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="ACHX5T_001170"
     CDS             complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="ACHX5T_001170"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR8466444.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30709..32229
                     /gene="aer"
                     /locus_tag="ACHX5T_001171"
                     /pseudo
     CDS             30709..32229
                     /gene="aer"
                     /locus_tag="ACHX5T_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32383..>32967)
                     /gene="nfeR"
                     /locus_tag="ACHX5T_001172"
                     /gene_synonym="yqjI"
     CDS             complement(32383..>32967)
                     /gene="nfeR"
                     /locus_tag="ACHX5T_001172"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR8466445.1"
                     /translation="QPRHEGCCKGEKSEHEHCGHGHQHEHGQCCGGRHGRGGGRRQRF
                     FGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYPTLDFLQEQSLITI
                     REEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQNPQMKRALDNFKA
                     VLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
BASE COUNT         8043 a         8499 c         8664 g         7761 t
ORIGIN      
        1 tttcaacggt tatgatgcag atgatgtcag tgacagtaat actgacgtca gcgatctcta
       61 ttatcgttat gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg acaaccgcac
      121 actatttaat gcacaaggcg ggaactttac ctttaacttt ctgccactcg gtgcaatcga
      181 tgggatgcgt atcgggtcga cactgagcta tttaaaccag gcgcaaagcc agcaaggaac
      241 cccagtaatg gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact
      301 tttgggatcg ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc
      361 aggcagctac agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct
      421 tgtgccgttt accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc
      481 aggtaaaact acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg
      541 cctgccatta catccgcaat atgagctcta cgcagggctg gcgaatgctg atgatgtgag
      601 tgctttcgag ttaggtaata actggacggc agatttaggc agggtgggga atcttgcaat
      661 cagcgccagc gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg
      721 gagtaatccg ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg
      781 tacaaccgac agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac
      841 agtttcactg aattttgtcg gctggaatat gatgctgggt tatacctgca cacaaaataa
      901 cactgatgat agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca
      961 gacaacagcg caaagtatct ctgaaactgt acaacttagc gcttcccgcg ctattgtgat
     1021 gcgtgactgg attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga
     1081 taacgacgac aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag
     1141 caataacaac agccattcaa ccaatgtgcc tacggattat cgttatagcg aacaggatgg
     1201 cgatcaaacg tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga
     1261 acttggcgta accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg
     1321 ttgggatggg caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa
     1381 tcatgatcat ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta
     1441 tggcgttaat ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt
     1501 gaaaggcttc tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc
     1561 aggcagccga actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt
     1621 tcagtctggt tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa
     1681 catcattaac ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg
     1741 cgaagtgtct gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt
     1801 gaaaaaattc ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg
     1861 atttacactt gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt
     1921 ccttaagtgt ccactgagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt
     1981 tacttgtagt gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt
     2041 gaaacagcct aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga
     2101 aatcgattga ttgcggcgat attgggtttg tttggcacgc tcactggcgt tcaggcagct
     2161 cctgacgtga ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc
     2221 tggaaggatg aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct
     2281 gacctccaac aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact
     2341 gaacaacgca gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta
     2401 agtagcgatg aagcgccgct aggatgcaag gggggcaaag ggcttcagac gaatcttaag
     2461 gattctaata gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag
     2521 tttcttacgc tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg
     2581 aaggggaaag ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc
     2641 ctgaatacgc tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg
     2701 ccaagggtgc agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa
     2761 gatctgacta tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat
     2821 gaaatcatgc tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct
     2881 atctatcgtc agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa
     2941 atgtacaacc cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg
     3001 aacagcatta acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg
     3061 atgtgtgtgc cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag
     3121 gccgggtact acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat
     3181 taaccctgca aaccggacaa acgctttttt ctatataaac gacgaagccg cctgataagg
     3241 cggcttcgtc gttataactt acgttaaacg ttaatgttgc ggtgcgggat cgttatgtgt
     3301 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
     3361 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
     3421 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
     3481 gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc
     3541 agacagtgcc tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
     3601 ggtgaacaga ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat
     3661 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
     3721 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
     3781 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
     3841 ccaggccggt tctgcaagga aacgcggcag tgcgataccc cagaactgac ggttacgcag
     3901 gatctgacca acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt
     3961 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
     4021 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
     4081 ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
     4141 agaacctacg ttaaagtagc ctactgcgat ggaacgctct ttcgccggga accattcgga
     4201 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc
     4261 aacagctagg ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag
     4321 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac
     4381 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
     4441 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
     4501 gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat
     4561 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
     4621 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaaaccg gcacgccccg
     4681 aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc cgattcatct
     4741 aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga cggcagaaag
     4801 ataacttgtc atacaacctt aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg
     4861 gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagcgaga tttaccactt
     4921 tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc ttcggactct
     4981 ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct cactcgttga
     5041 gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata ccgaatttgc
     5101 ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc attgccattt
     5161 gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca tctggttgaa
     5221 gggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggctgagc gtctgtgtac
     5281 tggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc cgcacactat
     5341 cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgttcg gtatcactgg
     5401 caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg aactgctggc
     5461 gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac
     5521 caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag acggctcttt
     5581 caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaatatcg aacaggcgac
     5641 ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc gccgctttgc
     5701 tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt
     5761 gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg aactggacag
     5821 cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa
     5881 aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg tacagcagta
     5941 ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg ggccggatgt
     6001 aggctttgac tccatcaacg accgtccgat ggcggaagag ttgtctaagc tgctgagcaa
     6061 gcagaacgaa gaaaacctgc tgccgaaaac cattctctac tgcctgaacc cgcgcgataa
     6121 cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca
     6181 gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc agatgaccca
     6241 actggcgcag ctcggtctgc tgagccgctt tgtcggtatg ctgactgaca gccgtagctt
     6301 cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga tcggtcgctg
     6361 ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg tgaaaaatat
     6421 ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc tggggttgat
     6481 atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc agatttggct
     6541 gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga tgttgctcgt
     6601 ggacataaat ttgcgttaac ggatatcgca aaaggggcca acgtcatcaa atatggcctg
     6661 ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc ccacaatacg
     6721 cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ttgattttca ggatctgcct
     6781 gcgcaagcgg cagatcctga agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg
     6841 cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc
     6901 cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta ccgacggcgt gttcctcttc
     6961 agccacacct atggctgctc acaactgggc gacgatcaca tcaacacccg caccatgctg
     7021 caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa
     7081 aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat
     7141 ttcatgatct gccaacagca ggatgatgag atcgaagccg ggatcgagca tttgcatcag
     7201 ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga actgaagttt
     7261 ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc gatgctgggg
     7321 cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga agtgccggag
     7381 atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac gtttgaaaaa
     7441 ctggtcacca tggtcaacga cttcaaacag tactttattg cccatgatca gccgatctac
     7501 gaaaacccat caccggggaa caaagcgggc ggtatcacca cgctggaaga caaatcactt
     7561 ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt
     7621 ctgaaaacgc cagggctgaa cttgttaagt gcgccgggta acgatgccgt agcgaccagc
     7681 gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgcgg cacgccatac
     7741 ggtggatttg tgccgacggt gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa
     7801 cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc gcagttgctg
     7861 gaagaattta tcgacaccat cgttgagttt gccaacggta agcaaacctg caacgagcgt
     7921 aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag
     7981 ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc atacctgcta
     8041 aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg cctttctgat
     8101 tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta
     8161 tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg gtgccagtgc
     8221 catccttaat gccattcgta cattgattac cttacgcacg cgcagcttat gggtaatggc
     8281 gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact
     8341 attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta aagggctcac
     8401 catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact tctgggcggg
     8461 gtcgataggc ggcacgatga ttgagggtag ttttctgctt atgaatggcc tgaatatcat
     8521 tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga aaacccctcc
     8581 cgccatagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc cagacgatcg
     8641 tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt
     8701 tcgcaagagt cctgcaatac gccgataata aagccgacgg caaccacctg catggcgata
     8761 tcgttcgaaa taccgaacat attacaggcc agtgggatca gcagcaaaga accccccgcc
     8821 acgccggatg cgccacaggc acacagagaa gccactacgc tcaacagcag cgctgtgggc
     8881 agatcgaccg gaatacccag cgtattaacc gcagccagcg tcaacacggt aatggtgatt
     8941 gctgcgcccg ccatattgat ggtggctccc agcggaatag aaactgaata ggtatcgcga
     9001 tccagattca gcttttcaca cagcgccata ttcaccggaa tgttcgctgc agagctgcgg
     9061 gtgaagaagg catacacacc gctttcgcgc aggcacagca gcaccagcgg gaacgggtta
     9121 cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag taacatacag
     9181 ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc
     9241 agggtagaag aaaccagccc aaaaataccg aacggtgcga agcgaatgac cagtttcacc
     9301 ataaaggtaa cggcattcga catatcattg accaggtttt tggtggtctc gttaccgtga
     9361 cgcagtgcga agccgaggcc gatcgcccac accagaatcc cgatgtagtt acctttcagc
     9421 agggcgtcga tggggttgga aaccatgctc attaccagtc cgcgcatcac ttcgacaatg
     9481 cctgacggcg gcgaaatatc acccgcgcta ctggacaagt gcagggtaga agggaaggca
     9541 aagctgaaga ctactgcggc cagagctgca gagaaggtgc ccagcagata gaggaacaaa
     9601 ataggacgga tattggtttt ctgcccgtgc tggtggttag caatagatgc cattaccagc
     9661 atcaacacca ggatgggggc aacagctttc agtgcgccga cgaacaaagt acctaacaga
     9721 ccaacagctt ccgccgcggg ttttgagatc catgccagaa gaatccccag aacaaggccg
     9781 accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc cggtgaacgt
     9841 tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac atccctggtg cgttctgtcg
     9901 acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa agttacagct
     9961 tcgggaagcg aaaaatgctg gattttaaag agaggtcatg ttttgcaact taatggttgc
    10021 atttatttaa cataaacaga gggaaggact ggctactaag gctgattaga taacatatgg
    10081 gcaaaactat ttggaatatg agaacggttg cgctccttta acgtgttatg ccatataaag
    10141 tcactggcgc agggagcgcg cagggggcgg ccaatcgccg ccgccccctg cacccccagg
    10201 ctctggcgaa caaaatcgcc gctgcgcggt tccctcagcc tatcccttgc ggctaccggg
    10261 tcgggcgcaa ggtaacgtcc ctgtaagatg cgccctcgcc ccggcctcca gggaacgctt
    10321 cggcgatttt gacgccacca acacccgtgc ggtctttttg taaagagcta taagccgatt
    10381 acataattaa tagctcacct ttgtctggaa tgagttatct ctaggaatgg cttaatatac
    10441 agcgaatcat tctccccgct gcttatcatg ccgataattc acccaggcgt tgataataaa
    10501 cgtcatcacc agaatgccaa acaccacgcc cagcgagacg gcgattggaa tatggtagaa
    10561 gtcgacaatc agcatcttga taccgataaa caccagaatc accgccaggc catatttgag
    10621 catcgagaaa cgctctgcta cgcccgccag caggaaatac atcgcacgca ggccgaggat
    10681 tgcaaacagg tttgaggtca gcacaatgaa cgggtcagtg gtcacggcga agatagccgg
    10741 aatgctatcc acggcaaaaa tcacgtcgct caattccacc agaatcagta ccagcatcag
    10801 cggtgtggcg tacagcaatc cattcttacg cacaaagaaa tgctcgttgt cgatggtgtc
    10861 ggtcatgcgc aaatgaccgc gtagccagcg caccagcggc ttgtcgccaa tgcctgattc
    10921 atcttcatgg gcgagcgcca tcttcacgcc ggtaaacagc aggaaggcac cgaagatata
    10981 caggatccag tcgaactgcg aaatcagcca gctgcctgtg aagatcatga tggtacgcag
    11041 aacaatcgcc ccgagcacgc catacaccag cacgcgacgt tgtaatgccg ccggaacgga
    11101 gaaatagctg aacaacatca gccagacaaa gacgttatcg accgccagcg atttctcaat
    11161 cagataaccc gtgagaaagg ccagggcctg tggatcggca acggcgcgac cttcggtttg
    11221 caccagatac caccagaaag cggcgttaaa cagtaacgac agcgtcaccc agaccagcga
    11281 ccaggccgca gcctgtttca tggtcatggc atgtgcccca cgacgcccct gcaacaacag
    11341 gtcgatagcc agcataatgg cgacaacaac agcgaatccg ccccatagca acggcgtgcc
    11401 gacagtattc atagaagttc cttacacata aaaaaacggc caacgtcgga agacgtcagc
    11461 cgctgctttt tatgcataga cctcgccttc cggcaaggtc tcacttacaa caaaaaagga
    11521 atacgtctgc gtattccttt cgggttgccc ggtgaccgga tgtggttttt cactcatcgt
    11581 aatgacgatc gaccggcaat gaagttactc ccctttgcag gtaacaaagt atgacagacc
    11641 attcagtcag tcaatggtct gttacatacc ttttacactg ttttactttg gcaagtatag
    11701 ttttacgcta tctgccggaa aaatcacccc agtttggcgg cggatctcgg tcagcagttt
    11761 ggctgtgatg cgactgaccg ccagccctgg atgatccacc agatgctcat ccaccagggt
    11821 agcgaacagc tctgcttcgt agagcatggt attaatatgc tgcggctggg tgagatcctg
    11881 catctggctg ccacgcggca caaagcagac tttctggcat tcagacagtt tttcaatcac
    11941 cagcgatccc gcttcgccct gaatctcgct tcccaggaca gaatcactga cctttgagtg
    12001 ctgcaaggtg acgctgaaat caccgtaatc catcaccacc acgccgtggg cgtcaacgcc
    12061 gcttgccagc aaactcgccg ttgcctgcac gcttttcggc tcaccaaata acgccaccgc
    12121 cgacgccagg cagtaaaagc cgatatccat aattgaaccg ttagagaatg ccggattaaa
    12181 ggtgttggga ttctcaccgt ccaggtaacg ttgataccgc gaggaatact ggcaatagtt
    12241 gaaaaagact ttacgcagtt tgcctgcttt cggcagcgcc tggcgcaaca aatgaaagtt
    12301 cggcaggcag gcggttttaa atgcctcaaa cagcaccacc tgattttcgc gcgcacaggc
    12361 aatggcggca tccacttccg ccagattcga cgccagcggt ttttcgcaaa tcacatgaat
    12421 tttatggcta aggaaaagtt gtgtctggga aaagtgcagg gaattcgggc tggcaatata
    12481 caccgcgtca atggcatcgc tttccgccat cgcttccagc gacgtaaaca gatgctcgac
    12541 agaaaaatca ttggcgaagt gctgagcctg ctcaaggctg cgggaatata cggcggttaa
    12601 cttgtattta ccgctctcat gggcggcctc gacgaactgg cgagtgatcc agttcgtacc
    12661 aatcacagcg aaacgtatca taacgcgtgg atactccata aagggattct gtcgtcaatg
    12721 tagcacgcct ttttcatctt cgacgtgcgc agatacgcat tattctttcg ccggacagcc
    12781 atgctcacta aacggcccaa tcatgaccga tggcccgaga tatttcggct ggcgatggag
    12841 gatttttgcg tcaatcaccg cgctgacacg agcgagtttc tcccgcagtt gagtataaaa
    12901 accgtgcttc atattgaggt ctggcgcgtt aaacccgata gcgtcgatgc cgtactgttt
    12961 tgccagccag atggtgcgtt ggttatgaaa ttcttgcgag ataatggtga tatggttttc
    13021 cccaaacacc tttttggcac gcactaccga atccagcgtt gagaatccgg catagtcgca
    13081 aaagatcact ttcgccggta cgcctttagc gatcaaagcc tgctgcatac ctgatgcttc
    13141 atcgtaattt ttacgtccgt tatcgccact caccagtagc catttcactt ttccggcgtg
    13201 gtacaacgct gctgcggtat caatacgtcg ggtaaagtag cgattacccg gcctcgcccc
    13261 cagcaataaa cccacgttac gcgccgggac ggcgttgaca tcgccccagg tcagctgctt
    13321 gctggcgttg accataacgc gatcggcgat aaaaatggtc gctcctgcca caagtaacag
    13381 taagcaggca atctttagag tccggcgaga aaaacaaata cggagaagaa ggcgggcaaa
    13441 tgcgcgcagc atggtcgtta tccttaacga aaggggggag gtcgatttta ggcgctaaag
    13501 aaagaaaaga taacgcctga catcattttc agattttgtc ctgcccaagc gacaactgcg
    13561 ttagccacag ccgggtgtcg aactcaagct ggtggtactg cggctccata tggcagcaca
    13621 attgatagaa cgctttgtta tgctctttct ccttcaggtg cgccagctcg tgcaccacga
    13681 tcatgcgcaa aaacggttcc ggcgcgttgc gaaacacggt agcgacgcgg atctctgctt
    13741 ttgctttcag cttgctgccc tgcacacgag aaacagcggt atgtaacccg agcgcgtttt
    13801 ttagcacatg gatcttattg tcatacatca ctttattgat cggcggggca ttacgcagaa
    13861 actgattttt cagatcctga gtatattgcc agagagcttt atcggtggcg tagtcgtgtg
    13921 ttcccggata gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc agtgttcgca
    13981 cctgcgaaag cagctgctcg ggataaccct gaagataagt aagattgctc ataaccgccc
    14041 acgaaaaaga gaaaaagggt atactcacgc acccttttca ggggcaacgt cgaaatttta
    14101 tcattcagga gggccgatga gccacttaga caacggtttc cgttcactga cactacaacg
    14161 ttttccggcg acggatgacg ttaacccgct acaggcgtgg gaagcggcag atgaatattt
    14221 gctgcaacag ttggacgaca cagaaatcct cggcccggtg ttgatcctga atgatgcctt
    14281 tggtgcgtta agctgcgcgc tggcggaaca taagccgtac agcattggcg actcatacat
    14341 cagtgaactg gcgacgcgcg agaatttacg cctaaacggg attgatgagt cgagcgtgaa
    14401 gtttctcgac agcaccgccg actacccgca acagccgggt gtggtgctga tcaaagtgcc
    14461 gaaaacactg gcattgctgg aacagcaact gcgtgcgctg cgcaaagtgg tcacgtcgga
    14521 tacacgtatt attgccggtg ccaaagcccg tgacattcac acttccacgc tggaactgtt
    14581 cgaaaaagtg ctcggcccga ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa
    14641 ttgcacgttc aatgaaccgc cgctggccga tgcgccgcag accgttagct ggaagctgga
    14701 aggtactgac tggactatcc ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat
    14761 cggcgcgcgc ttctttatgc aacatctgcc agagaatctc gaaggggaga ttgtcgatct
    14821 cggttgcggt aatggcgtta ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt
    14881 ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa
    14941 catgccagag gcgttggatc gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga
    15001 gcctttccgc tttaatgctg tgctctgcaa ccctccgttt caccaacaac atgcgctgac
    15061 cgataacgtc gcctgggaga tgttccacta cgcccgccgc tgcctgaaaa tcaacggcga
    15121 gctgtatatc gttgccaacc gccatctgga ttacttccat aaactgaaga aaattttcgg
    15181 caactgcacc actattgcga cgaataataa atttgtggtg ctgaaagcgg tgaagctggg
    15241 acgtcgtcgg taagattggg agttacctgc cggatgcggc gtaaacgcct tatccggcct
    15301 acgttcagca cgttaccccc aggccggata agacgcggca agcgtcgcat ccggcaatgt
    15361 ctgctcagtt ctggaatcgc gcttccataa aacaaaaaat ccctgtcacc atcctctaca
    15421 ttctcttgtt tagcgttttt ctacgtttat tcttccgtca cacagataaa ttcatccgtt
    15481 gccaatctgt caacggatgt tatcatattg ccaatttgtc aacggacgtg atgaagaatg
    15541 cacctgataa ctcaaaaagc attgaaagat gctgcggaaa aatacccgca acataaaacg
    15601 gagttggtgg ctctggggaa cacgattgct aagggatatt tcaaaaaacc tgagtcatta
    15661 aaagcagtat tcccatctct ggataacttc aaatatctgg ataagcatta tgttttcaat
    15721 gttgggggca atgaattacg tgttgtagca atggtctttt ttgaatcgca aaagtgctac
    15781 atacgtgaag ttatgacgca taaagaatac gatttcttta ccgctgttca tcgtactaag
    15841 gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagcta actgctgtgg
    15901 caccttttct ggcgggtatt cagaacgagg aacaatacac ccaggcgctg gaactggtag
    15961 atcatctgct gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa
    16021 ccgcgtggga agaatcagcg cccgaatttg cggaatttaa tgccatggct caagccatgc
    16081 ctggcggtat agccgtgatt cgtaccctta tggatcaata tggtttaacc ctttccgatc
    16141 tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag aggaaattaa
    16201 cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc ttgtttattg
    16261 attaacgcga cgtgccgaat gcgttgcatc cggcgcttca aataacgatt taaatctcca
    16321 gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc cagctccata gccacatcgc
    16381 agccgccaat taaatgcaca gttttcccgc tatcaatcag cggttgcgcc agcgcgcggt
    16441 ttggctcttg ccctgcgcag atcaccacat tgtccactgc taatacctgc gtttcgccgt
    16501 tgatcaccac atgcagcccg tcatcgtcaa tcttctgata acttacgcct gggatcattt
    16561 tcacaccccg cgagagcagg gtggtgcgat ggatccagcc ggtggttttg cctaacccct
    16621 gtcccggttt gctggctttg cgctggagca tcacaatttg ccgtgggcta cgcgggatct
    16681 gcattccctg cgggcttaag ccaccagcct gttgtaggct gctgtcgatc ccccactcat
    16741 tacagaacct ggcgatattc tggctggtgg attcgcccgg ctgacttaaa tacatcgccg
    16801 tatcaaaacc aatcccgcca caaccgatga tggcaacctt gttgcctacc ggcgctttgt
    16861 cgcgcagtac atcgagataa ctcaatacct tcggatgatc gatcccgtcg ataggcggaa
    16921 cacgcggcac gatcccactg gcgaggatcg tttcatcgaa cgcctgtaac tgatccgccg
    16981 tcacggtgtg attgagtttc agcgtcacgc ccgtcacttc gatcatccgg cggtagtagc
    17041 gcagcgtttc gtaaaactcc tctttgccgg ggatctgttt ggcgatatta aactgcccgc
    17101 cgatctcgct gtgcgcatca aacaatgtta cctgatgccc acgcgccgcc gcgttaatgg
    17161 caaacgccag cccagcaggt ccagcgccga ccaccgccag attttttttc tgcacggcgg
    17221 gaaggattgg cattttggtt tcgtggcagg cgcgaggatt caccaggcac gaggtgactt
    17281 tgccaacgaa gatctgatcg aggcagccct ggttgcagcc aatacaagtg ttgatctcat
    17341 cggctcgccc tgattgcgct tttgacagca gttccgcatc cgcgagaaac ggtcgcgcca
    17401 tcgatactat atcggcatcg ccgcgcgaga gaatatcgtc ggcaacctgc ggatcgttaa
    17461 tccggttggt ggttaccagc ggcagcgaga cgtggccttt cagtttgcgc gtgacccagc
    17521 taaatgcgcc gcgcggcacg ggcgtggcga tggtcggaat ccgcgcttcg tgccagccta
    17581 ttccggtgtt gataatggtc gcgcccgccg cttcaatggc ctgcgccagt tctaccgttt
    17641 cggcaaaagt gccgccgcct tcgactaggt cgagcatcga caaccggtaa ataataatga
    17701 agtcgttgcc gacgcgttcg cgcaccgtac gcactacttc tacggcaaat cgcatccggt
    17761 tgcggtaatc gccgccccac tggtcactac gctgattggt gcgcagcgtc agaaattcgt
    17821 tgatcaaata cccttcagag cccatcactt cgacgccatc gtatccggcc tcccgcgcca
    17881 gttgcgcgca gcgggcgaaa tcgtcgatca gttgcaggat ctcttcatgg gtgagttcgt
    17941 gaggaacgaa acggttgatg ggggcctgca atgcggacgg tgcgaccaga tgcggttggt
    18001 agctgtagcg cccggtatgc aaaatttgca gggcgatttt gccgccttcc tgatgtacgg
    18061 cttcggtaat ggtgcgatgg tgtgggatct ggctggcgtc gttgagcatt gcgccacctt
    18121 ccatgccaac gcctgttaaa tctggtgcaa tgccgccgct gacaatcagc gccacgccgt
    18181 gacgggcgcg ttcggcataa aacgcagcca gccgttcagc accgtccggg tattcctcca
    18241 gcccggtgtg cattgaaccc atcaacacgc ggttttttaa cgtggtaaaa cctaaatcca
    18301 gcggggcgaa cagcgacggg tagctcataa aattgtccag tatgtaaaat aattgttatg
    18361 tagtcggatg agttctaatt tagccgtcga tggagaaaag ggaaaagggg agtgcggggt
    18421 ttgtgatggg attcaaaaag aatacgcccg gtggtaacgc cggatgcgac gcttgccgtg
    18481 ttttatccgg cttgggggat tgcacatgta gggcggataa ggcgttcacg ccgcatccgc
    18541 cagccgttgc aaattactgc ttacggaaaa aatcgttata caacatatac aaatgagcgg
    18601 cactccagga gaaatttggt gcgccttgct gcgcgcctgt cagcgggttg taattctcct
    18661 gaattgggcc atcggcggtt aatcctttgg cgtgcctgaa gaacgtatcc gccagcttca
    18721 gggcatcatc acgataaccg taacgctcca tccctttcag accaaaccag aactgatcca
    18781 cccatacgcg cccgcgccag tagatatcag cgccaaaagc cggattggtt aacgccgccg
    18841 ttcccagcgg aacaaatgtg ttgaactctt taggatcgag catcaccttc accaccgcgt
    18901 cggcattggc ctgcgttgcc gcaccgttaa acagcggcga ccagccttcc ggccctttac
    18961 cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag tggtttatct tcaatacgta
    19021 cgtcatagta gaactgggta gtcgggtcga acatacaggt gttgatgtag tccgcgagct
    19081 gctgtgccaa ctggcgatag cgtttggcct cttctggctt accgaggatg gttgccatct
    19141 ccgccagata atggttatcg ctgtacatat agctggcctg atccaccgac tcctgcaata
    19201 gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc ggcgaatttc accgtccagt
    19261 cgctacgttt gccgccatta gcgacatatt tatccaactg ttctttgtcg ataaaaccaa
    19321 agacggcggc atcatcacgc cccgattccc acgacgcagc gacctgtgcc ggaatttcca
    19381 gactgtcgta ctggcctttc tccaccacgc gggcgtagtt gttcagccca gactgcgtct
    19441 cttctttatc gccttttttc accgtaaaca gcatctcgcc gctctcggtg ttgtgggctt
    19501 tgtcgcgggt cgcgccatat tccggcaccc cgttgccgtt atgatcgcgg ttacgtaacc
    19561 accagtcgtg ataggccacc agtttcgggt acatctctgc caaccaggct ttatcctggg
    19621 tgacgttgta tacttccatc accgaccagg cggcaaggct gggtttggta ttacgttcgt
    19681 tccagttgcc gccatcgcca ccacgctcgg ggctaaggtt ccacgctatc aggtcgggga
    19741 caaagcccac atcctgcgga cgcacgctgt cgccaggctg gatctgccag gagaagaccg
    19801 cgcggatatt ctctttggcg atatccggat tgaaatgcgc catcgcaaac gcctgcttcc
    19861 aggtatccca cggccaggtc tgattgccgg agaaccagcg tccggtcacc gacggcgtga
    19921 cggtgttata attcaccgca ccgcccggtg agcgccagtt accgttgagc gtttcgatcg
    19981 ctttcaccgc gacgcgcgtc tgttcctgcg tcgcatccgg attggttaac cctttcttca
    20041 ggtattcttc ccagcgttgc tgcgaggcgg tgagataaaa cgccggacgc gccagaatat
    20101 cgcggatctg catttgctct ttgctaactt cctgagcggt cagcagatgg gaataggtgg
    20161 tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt aaagcgatta ccgttgattt
    20221 cagtctgcac cggcagggat ttatgcacct gatattccga ttcgccggag gtcagcagat
    20281 cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc atcacgggtg gcgctgattt
    20341 tgcgctgata gtcagggtat tcgccagcaa tggttttatc ggaaagcggt ttcccttctt
    20401 tcgcttccag tttttccagc agttcgccat cccacaccag atccagcggt ttattgctga
    20461 tgattttagt ttccagtagt gacgtgcgcg gcgtggcgaa gcgcagagtc atttcgacct
    20521 gcacatcttt tgctgtcagt ttttgcacca gcgcgccggg aatactgtat gcctccagcg
    20581 tgaagttgac tttcttgccg tcttgccaga cagtcaggcg gtcgaaattg ctggccataa
    20641 agttgatgta ttcttccgtc agcagcgcaa cgcccggaaa gccgcccatg gtgttcgggc
    20701 cgtctggcaa cagatgacca tgccaggctc cgagatcgaa aaacggatta aaacgctggt
    20761 gatcgtcgta atcgtaatcc ttcatgtact gcggcgcacc agtacggtta atcacgtttt
    20821 tataattgtc ggcgttagcg gcatgagcgg aaaaacttat cagcagagcg caggttactg
    20881 gcgttaaaat agttttgatt ttcatgagat tacagtcctt attaccagta gaaatattgc
    20941 atcaggaaga tttggtcgct gggatcgtta gaggcatttt tcatgtagaa gcggctatca
    21001 aaagccgtgg cgaaacgtcc gccgccatat tcgtaccaga taccaaactg catgaaagag
    21061 gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt aggtgtaagc ggtggagaga
    21121 taaacgcctt ttaacgcttc atacatggcg ctcaccatca tcccgctttc acttcccggt
    21181 acgtccagcc cgtcggcacg tccggtatgg tgccaggcgc gagcggcaaa gttaggggta
    21241 aacaggcccg tgagatagag ttgtcctgtg ccgtcggtaa tttccagacc attcatccag
    21301 gtgatgcctt cggcaaggtc atactggatg taaccgttca tccattgccg gataggtgta
    21361 tttgtcgtcg tagcgatcgt acttgccaaa atgtagccag gctttgcttt cgtcatgatg
    21421 tccggcaggt gtggcggtga cgctataacg aaagttgccg cttaagttct gtaccttcag
    21481 cgcggtcata atgtcgcggg tattgggaat gacgtagccc aaatcgggtg tgaagtcgcc
    21541 ccaccattgc aggtcatcca gcgaggaatc ctgacgcaga ctgagcatga tttcggtgcc
    21601 atcattggta cgataaccgc catagaaacg gttaatccca ccttcgaaac caccccagct
    21661 atggtcaggc acctaggcat taccgtcgtg gtcggcctgc acggtccagc cttcgccgta
    21721 aatcaggccg aaccaggagc cgtacttcat ctccaggcca ccttcaatat aggtgccgtc
    21781 gctgtattta tgtttattct caccctctaa atccatatat ccaccaagcc ctaattcgcc
    21841 gtaaaaacga aatgaaggag aagatgtcga ggtaattgcg ggtgtttttg tgtcgtcatg
    21901 ttctgccgga cgtgcttctt ctcctgccgc agaaaaggcg tagcaaaacg gcagagcaag
    21961 taatgctctg catggtgcag tgattaattt cattgtgtta atccatgagc taaagaaaaa
    22021 gtacagcctt ttatcgttgt aattataaaa atataaaggc agaaactatt ttccctctcc
    22081 tcatccagga agagggaaaa tgcctgctca ttttttatta gtctgcttaa tcaacattgc
    22141 tggctggcgt cgcttcaata tggcgctctt cagccgttaa tccctttata tatttgctgt
    22201 atttccacca cgcaaagccg aggaagataa caataccgcc gacgttataa aaaatgatgg
    22261 tcagaatatt cgcgccagtc gggaatgtcg aagcgacaaa ccctacggca aatatcgcaa
    22321 tcagcattga aacaacgata ataccggtgc ggcgggagcc catacggaaa tcgcgtggca
    22381 ggtgatctaa tttggcgcgt aaattcaggt aagccagcat gatgaataac ggcggaagca
    22441 tggacgctgc ggcggtcata ttaataatgg tattcatcag atcctgcacg gtattggaac
    22501 cgagcatcgg gataatcatc agcgggatga cgatcagaaa ctggatccat gctgcgcgcg
    22561 ccggaacgcc gttttcgtta agttcgacgg ttttcttacc aaagatgcct tccgggattt
    22621 cggagaagaa aattttcacc ggcgttgcgg tccacatcag cagggaaccg aacattgcgg
    22681 taaaggacac cagaccgaca aagcgattca tcaacgcttc cggcagacca aaatacgccg
    22741 ccatgccgtg gaatacctgc accgatccgc cggtaaattt caactctttg ctgctgacga
    22801 agacgttaat cagcaccgag gagacggaat acagtacgcc gataaaaatc ccggcgagga
    22861 tgatcacttt aacgaacgat ttcgaaccgc ctttgacgtc gttaacgtac accgcgacgg
    22921 actccgcgcc acctgcggcc ataaagatcc aggtggtaac gccgaggaac gcccagttga
    22981 agttcgggat catcgcgtca acggtgatcg gatcggcagg ctgtacgccg ccaaccagtg
    23041 ccgtacctgc cagtaaaatg taggagagcg ttaacagcag catcagcgtt gaagtgacgg
    23101 aggtaattgg ccccagcatt ttcgccccgt tggtggaaac ccaggtggag aaggcgaaca
    23161 gcaccatact gataacggtg gtggcaaccg gcgtcataat atattcgtag ccgaggaagg
    23221 catacgaagc ataagcaata acgcgcggca acagcgaggt gaaaaagaac agatttacga
    23281 accagtaagt gtaggcagta ataaatgccc aacgtccgcc cagcgaactt tttacccacg
    23341 cgtagacacc ggcttctgag tttttattta acgaaacaaa ttctgcgatg atcagacaga
    23401 agggaataaa ataaaaaatc gtcgcgagga aaaacatcgg tgccgaggcc agtccaagct
    23461 caatattatt attgataacg ttgttaaagc tgtaaacggc ggcaaaagtc agcgagagca
    23521 agccgaattt gccgattgta ttacgtttgg tatcagacat aacacatctc cgaaaatgtg
    23581 tagggtagcg gtggcggcaa actccttttg ccgccgtagt tgttatttgt tatggaaata
    23641 accatcttcg atggtgactt tgagaaccac ttttttgacc gcgttattgc agataaaccg
    23701 atacgcttca tggatatcgc aaataacgat ttgcccttcg tggacctcta cggtttctcc
    23761 gcagcctttt aaatattcac ggtcagtttc atcacgataa tattccacta cctgtaatgt
    23821 ctctttcggc gcatattcaa ttttttgctg cccttgcagg tagtaatgca cttcaaaata
    23881 gcgtcgatgc ccggtaaata gcgcatcggt cgcggagtca ttttccacac ggtaggtcaa
    23941 tgaatcaccg atggagtggg cgacgccagg ctgaatgttg tcgatatttt caatcgcttc
    24001 aacgcagcgt tgccacttct tgccagaggc gtaaatctgg cggaactgtt ctaagttatc
    24061 gatgatcctc attgcttatt ctcgctgtgc aaattcgtgg aaaagaaccc tgcgccgaac
    24121 tcatacgacg ccaggctttg cgcggtagct tctccgccag aaaccggcag caacgtaaag
    24181 ccgtagctga agtcacggaa ccagacgcgc caggagtcca gcacctcgct gccccaggag
    24241 ttggagccga ggccaagtag ctggtggtcg aggttcaggg tgatgtcatc actgcgctgc
    24301 agctcgttac agtgctgggc agcgtggatg ttttcctggg tatagtgcca ggcgctgaag
    24361 ttaattgggc gctgcggaac caccagcaga ccgttgccgt ggcggttagt cagtgccgtc
    24421 cagcggacat gctgacggtt gccgttgttc tgcgggaagg gatagttctc gaacatggca
    24481 tcgacggtgc tgcgccagat atcgatgatg ttagcctgct ggctgtcggc gtagttttcg
    24541 cccggtccac gaccgtaata cgccacctga tcgtattcgc cgttaatccc catggtgaaa
    24601 ccgatgcacg gaatgatgtg cggatagtcg ccgtaacgct cgccggaaag cgccacgtta
    24661 acctggccat cggcagcgat gcgccagatg taggtacagc gcatcccgaa gtcaaacacc
    24721 ggcggtgcaa taactgtgcg gctgatgatc aacacttcac catcgctctg ttctacggca
    24781 aagtcgcgca gatgttcctg catgatctgc aaatgattcg gctgccacaa cccttcgtac
    24841 tcctgcttgt ggttgtcgat catcggcttg aagaagttga tctttggctc gcgggtcagc
    24901 agcgactcgc catttacctg ccaggatgtc ggtttgccac tcatttttga gaaggtgatc
    24961 gcgaagttgt agccgcgaac ggtgcagctc aaacgatcgt cttccagcgt cagcggacgc
    25021 gcattatttg gtgcgaaagg cactggctgc gcggtgtttt ccttcagcgg gaactgataa
    25081 gtggcgatag aatgcccggc ttcgctgtag cgggtgcggg aatctttggt caccgtaatg
    25141 ttgaggaacg cttcgcgggc gtccagctgc ggcagcgtga tctgcaaggg ggcttcgctg
    25201 ttcggcgcaa cgtcgcgcag tttaatctgc tgcgtcgcga gcgtttcacc ttcggcgcgc
    25261 acctctgcgt gcagggtgta gtcatcaagc gtggtaaacc acagtttatt ttcgactttc
    25321 aactcgccgc gagtcagcgc gtggattttt accggcgcga taacctgttt gtactctttc
    25381 aggcccgatc ccggcgtctg atcggaatag atcaaaccat caagacagaa gttatagttg
    25441 ttgggatagt cgccgtagtc gccgccgaat ttataccaga cattgccgtg gtcgtcctgt
    25501 gcctggatcc cgtggtcgca ccactcccag acataatgac cctgaatgca atcgtgctta
    25561 tagaagacgt tctggtactc cgtcagcccg cccggtccgt tccccatcgc atgagcatat
    25621 tcacagatga tgcgcggctt cggatgcggg tattcaccaa actcattcat cagcggcacg
    25681 cgggtgtaca tggtggaaat aatatcgacc acttcagcat cgcgatcttc ttcgtaatga
    25741 accagtcgcg tgtcatccag cgctttcgcc gcatggtaca tcgcgcggat gttacagcca
    25801 tagccggatt cattgcccag cgaccagatg atgatcgacg gatggttttt ctgtgcgtgg
    25861 atatggcgaa caatgcgctc gacgtagact ttttcccact gcggatcgtc ggtaatacgg
    25921 ctgatatcgc cgacattagc aaagccgtgc gattcgacgt cggtttccgc catcacaaac
    25981 aggccgtaga tatcacacag ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc
    26041 acggagttga tattgtgctg cttcatcaac tggagatctt tctcgacgcg atccattcca
    26101 acggcgcggc ctttgcgatg atcgttgtcg tgacggttga cgccgtgcag catcacataa
    26161 cggttattga tccagaacag accgtcgcgc actttgatat cacggaagcc aacgcgttgt
    26221 ggcaccactt ccagaacgtt gccgttggcg tctttcagcg tcatgaccag atggtaaaga
    26281 taaggggatt ctgctgacca ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg
    26341 gtcagttttt caattgccaa atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca
    26401 aacagggtat attccagcgt cgtgacgaca ggggaggcgg cgagattttc cagcaccact
    26461 tcgcaggaaa gcgtggcatc gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg
    26521 ttaatatgcg ttaggtgttt tccgaccaga taaacatcgc ggaagatccc cgccgaccac
    26581 cacatatcct ggtcttccac gtaggtagag tccgcccact gcatcacgcg cacacacaac
    26641 aggttgtcgc cggttttaac catcgcgctg atgtcaaact ctgcggtcag gcgactgccc
    26701 ttgctgaaac ccacatactg accgttaacg tagacttcaa aataggtttc gacgccgtca
    26761 aatttaatca gcgtctgttt accctgccag ccgtcgctga gggtgaaaat acgttgatag
    26821 gcaccggttg ggttatcgct ggggacaaac ggcacatcga tggggaacgg aaaaccttcg
    26881 tcggtatatt gcagtttgcc gtgaccttcc atttgccaca tggcggggac ggtaatatgc
    26941 ccccagtcag ccattaactc agaggtgaag gcttctggta cttgcagcgg atggtcaaaa
    27001 aagtggaaat tccactgact gcttaagggc agaaacaggc tgctggtttc gcgggcaaag
    27061 gtacgcgctt gcgcaacaga atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt
    27121 tcgtgggtga gctgaatgtt ttcccagcga ttcatagggc ctcccggtag agagaaatcg
    27181 gaaccattca tggtgatggt agtttgtgta attgctgggg gtttagtaaa atttttacta
    27241 aaaagcagca ggtaaatgca tttactttaa ctggatcacg aaaataaacg gcagcactgt
    27301 ccggcaaaaa tgattgccga acagtgtgta aggggattta acgggtcgtg ccgcgcagtt
    27361 ttaatttgct gggaacgaag actagcagcg gcagcgcgcg accatcgcgg gctttttcat
    27421 acaccaggtt aacgccctga cttcccatca tctcggaatg gatgcgcacg gtggagagcg
    27481 gcggaaaggt aaatcgcgcg gtggggatat cgttaacgct gataagcgaa atatcttgtg
    27541 ggatgttcag gccacgttca tgaattgccc gcagtacgcc gatagcaatg gaatcggaag
    27601 caacaaacag tgccttcgga tagtcttccc gcgccagcat ttgttttgcc agttcataac
    27661 ccgacgaact ggaaaaaccg ccgcgccaga tatcctcttc gcgtaccact tgtttcagtc
    27721 ggccatattc cgcaaaggcg acctcacgaa tatccgcctt gccaggctca tcttcaccgc
    27781 caataaaacc aatacgatta acgccctggt tgatatagaa gtcgatgatt tctttactga
    27841 tgcgtgccag atcgatatcc accgcatcgt aaccgctgcc gggttcgtga aagtcgataa
    27901 aacagatatt gtcggtcaac gcgctggcag cggcgcgcag ggcgggcgtg ggtttgccga
    27961 caattaaaat accggtgacg tttttaatgt ctggtaatcc gttgtgttca taacagttgg
    28021 tcagttcgat cgccagcttt tcgcactgag tttcaatgcc gtggcggatc gccagatagt
    28081 aaggatcgtt gatctccagc tcctgctggt agctgtagat agccagaata tggtgttggt
    28141 tgactgcgcc tgtctgtagt ttacgggcgc tactggtctt gtactccagc ttttcggcga
    28201 tctcgagaat gcgatgtttc gtctcttctt tcacattcaa tgtcggatcg tcatttaaga
    28261 ccctggatac tgtcgccagg gatacgccag cttcgattgc gatgtctttt aatgttgcca
    28321 tgtttacctt ttttactttc cctgatgtgc gcccccattg tagaggaagt gcttatcggg
    28381 tcacctgttt tcagtaaata aagcgcgttt ctttgacgct gtaacggtaa cacagcggct
    28441 taggaatgtg atcacgcctg cattctccat ttttagccta caggattgag aaaaactttt
    28501 ctatagttta gtaaaatttt actcgaaggg aggaagcaat ggaaaccgtg gcttacgctg
    28561 attttgcacg tctggaaatg cgcgtcggaa agattgtgga agtgaaacgc catgaaaacg
    28621 ccgacaagct gtacatcgta caggttgatg tggggaaaaa acactgcaaa ccgtgaccag
    28681 cctggtgccg tactacagcg aagaggagct gatggggaaa acggtggtgg tcttatgcaa
    28741 tctgcaaaaa gcgaaaatgc gtggtgaaac gtcggaatgc atgctgttgt gtgcggaaac
    28801 cgatgacggc agtgaaagcg tgttgttaac gccggagcgg atgatgcctg cgggcgtacg
    28861 cgtcgtataa aaagatcgga tggcgacgtc gtatcgccat ccgatttgat attacgcttc
    28921 ttcgacactt actcgcatgg ccgccagcgc cttacgcgcc gctttgatca ccagttcaca
    28981 ctgttcaatg gtcagtgtca gtggcggttc aatgcggatc gttttggcgt tattgagcgt
    29041 tccggccacc agtacgcgct ggcggaacat ctcgctggca aagttatagc cgatttcgtt
    29101 atcaacaaac tcaatcgcca tcaacatccc tttaccacgc gcttcctgta ccagatcggg
    29161 atattcccgc gccagttgac ggaaaccgtc cagcaacata tcgccttttt gctcagcctg
    29221 agccggtaag ttctgctcca gcaacacatt gatggtcgcc agcgccgccg cacaggccag
    29281 cgggttgccg ccaaaggtgg tggtatgcag gaatgggttg tcgaacagaa ctgaaaacac
    29341 ctcttcagtg gcgatggtcg cgccaatcgg catcacgccg ccgccgaggg cttttgccag
    29401 acacagaatg tccggctgaa cgttctcatg ttcgcaggca aacatcttgc ccgtgcgccc
    29461 catgcccgtt tgtacttcat cgaggatcat cagtgcgccg aactcatcgc acagcttacg
    29521 cacggcggtg agatagcccg gcggcggcag aattacgcca ccttcgccct gaatcggttc
    29581 gaggatcacc gcagccacat catcaccggt ttttttgcac tcattaagag ccgtgcgcat
    29641 ggcttcgata ttgccaaacg gcacatgacg gaaccctggc agcagtggca taaacggttt
    29701 gcggaaggtc gatttcgccg tggccgacag cgcgccaagt gatttaccgt ggaacacgcc
    29761 gctggtggca ataaaagtaa atttgccgcg cggcgactga taagccttcg ccagcttcag
    29821 cgccgcttca acggactcgg tgccgctatt acagaagaag ctgtatttca gtttaccggg
    29881 cgttagcgca gcaagggttt tcgccagcat agcccgtaac ggatcgagta gctcctggct
    29941 gtgcagtggt tgtttcgcaa gttgattctg tacggcggaa accacaactg gattacggtg
    30001 ccccacgttg aaaattccaa aacctcccag gcagtcgata aactcctgtc cctgggtgtc
    30061 gacaagcgta tttaaacctc ccgcttgcca ctctacggct ccgtaatccc cgccggcggt
    30121 aacagatttg cgatactcta aaaaccccgg attgacatgc tctttgaagt attcaatcac
    30181 ctctcggtta agtgctttca tctcctcatg atccagcgtt cgcttctcaa tgagattcag
    30241 ggcgtgggcg ctgcacgcta aagccgatgc gctcgaaggt aacctgttca aaatatgctc
    30301 cggaggctcg cgtatcacat gataccgatt taagtattgc agggattgcg ccactccggc
    30361 tgcgatcgcg aaaatcggga taaacacaaa tgcggttatc atttgtgcaa tatttaatgc
    30421 tatccgttaa catttgttgt tattttgcac ggtttttgtc gggtatggat ttgatgattg
    30481 ctgcgcaaat gcacgttttc agggcaataa ttgcctgatt tttgggcaag ataattaacg
    30541 ttttcgtata gtaacagagt gaaagtattt taaatcaaag aattagattt gtgctttaat
    30601 cgcaaattgc gatctaaatc aaattaatcg gttaaagata accgcagcgg ggccgacata
    30661 aactctgaca agaagttaac aaccatataa cctgcacagg acgcgaacat gtcttctcat
    30721 ccgtatgtca cccagcaaaa taccccgctg gcggacgata ccactctgat gtccactacc
    30781 gatctgcaaa gctatatcac tcatgctaat gacacttttg tgcaggtgag cggctttacc
    30841 ttgcaagagt tacaagggca gccgcacaac atggtgcgtc acccggatat gccaaaagcg
    30901 gcgtttgcgg atatgtggtt caccctgaaa aaaggggagc cctggagcgg catcgtgaaa
    30961 aatcgccgca aaaatggcga ccattattgg gtgcgggcca atgcggtacc gatggtgcgc
    31021 gagggaaaaa tcagtggcta tatgtcgatt cgtacccggg cgacggatga agagattgcg
    31081 gcggtggagc cgctgtacaa agcgttgaac tccggacgta ccagtaagcg tattcataaa
    31141 ggcctggtgg tgcgtaaagg ctggctgggt aaactgcctt cattaccgct tcgctggcgg
    31201 acgcgtggag tgatgaccct gatgtttatc ttgctggcgg ccatgctttg gtttgttgct
    31261 gccccggtgg tgacgtattt cctctgtgtg ttagtggtat tgttggcaag cgcttgtttt
    31321 gaatggcaga ttgtgcgccc gatagaaaat gtcgcccgtc aggcactgaa ggtggcgacc
    31381 ggagagcgta atagtgttga gcacctgaat cgcagcgatg agctggggct gacattacgc
    31441 gcggtagggc agcttggcct gatgtgccgt tggctaatta acgatgtctc aagccaggtg
    31501 tccagtgtca gaaatggcag tgagacgctg gcgaaaggca ccgatgaact gaacgaacat
    31561 acccagcaga cagttgataa cgttcagcaa acggtggcga ccatgaacca aatggcggcg
    31621 tcggtgaaac agaactctgc cacggcgtcg gctgccgata aactttctat caccgccagt
    31681 aatgcggcag tgcagggtgg ggaggcgatg accacggtga tcaagacaat ggacgatatc
    31741 gccgacagta cctagcgcat tggcaccatt acttcgctga ttaacgatat tgcgtttcag
    31801 accaatattc tggccctgaa tgcggcggtg gaagcggcgc gtgccggcga acagggcaaa
    31861 ggttttgcag tggtggcagg ggaagtgcgt catttagcca gccgcagcgc taatgctgcc
    31921 aacgatattc gcaagctgat tgatgccagt gctgataagg tgcaatccgg ttcgcagcag
    31981 gtacacgccg ccggacggac gatggaagat attgtggcac aggtgaaaaa cgtcacccag
    32041 ttgatcgccc agattagcca ttcaacgctg gaacaggccg atgggctttc cagcctgacc
    32101 cgtgcagtgg atgagcttaa cctgatcacc cagaaaaatg ccgagctggt ggaagagagt
    32161 gcgcaggtgt cggcgatggt gaaacaccgc gccagccgac tggaagacgc ggtgacggtg
    32221 ctgcattaat cggtatgccg gatccggcgg ttggagcaca atgcctgatg cgatgctggc
    32281 gcatcttatc aggcctgcgg ggtgtgtagc gggtgtaggc cggataaggc gcgtcaagcg
    32341 tcgcatccgg caattgcacc gcgccactgg cggatgcggc gattaatcca gttgcgtaat
    32401 atcaaaagcg gcgcggtcga tcaccgcaat gatctttttt atttgtgcat cactgatatc
    32461 gctctggttg acgcgtaaat ccagcaccgc tttaaaatta tccagcgccc gcttcatttg
    32521 cgggttctgg cgcagcgccg cgccaacgca acgcgctttg atgcgttctt caatcatctc
    32581 cacttgttcg cggttttctt ccagccactg cgcgccttgt tcggtcagcg caatctgctt
    32641 cttaccacct tcctcttcgc ggatggtaat cagcgactgc tcctgcagaa aatccagcgt
    32701 cgggtagatg acgcccgggc ttggggtgta attcccctgg gttagattct caatcgcttt
    32761 aatcaattcg taaccgtggc tgtcatcgcg cgagagaata tccagaatca ccagacgcaa
    32821 ttcaccgtga ccaaagaaac gttgccgacg accgccgccg cgaccgtggc gaccaccgca
    32881 acattgaccg tgttcatgct ggtgaccgtg tccgcagtgc tcgtgttctg acttctcacc
    32941 tttgcagcag ccctcatggc gtggctg
//
LOCUS       DAZSIO010000027        32086 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZSIO010000027 DAZSIO010000000
VERSION     DAZSIO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.027"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..108)
                     /locus_tag="ACHX5T_001173"
     CDS             complement(<1..108)
                     /locus_tag="ACHX5T_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466446.1"
                     /translation="MLRMFIPTSNGKISRHRYIFLFILINFIFAFLIIFF"
     gene            complement(203..1093)
                     /gene="rarD"
                     /locus_tag="ACHX5T_001174"
     CDS             complement(203..1093)
                     /gene="rarD"
                     /locus_tag="ACHX5T_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR8466447.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(1145..1612)
                     /gene="yigI"
                     /locus_tag="ACHX5T_001175"
     CDS             complement(1145..1612)
                     /gene="yigI"
                     /locus_tag="ACHX5T_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR8466448.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1777..2646
                     /gene="pldA"
                     /locus_tag="ACHX5T_001176"
     CDS             1777..2646
                     /gene="pldA"
                     /locus_tag="ACHX5T_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR8466449.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2779..4608
                     /gene="recQ"
                     /locus_tag="ACHX5T_001177"
     CDS             2779..4608
                     /gene="recQ"
                     /locus_tag="ACHX5T_001177"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR8466450.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4672..5292
                     /gene="rhtC"
                     /locus_tag="ACHX5T_001178"
     CDS             4672..5292
                     /gene="rhtC"
                     /locus_tag="ACHX5T_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR8466451.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5354..5974)
                     /gene="rhtB"
                     /locus_tag="ACHX5T_001179"
     CDS             complement(5354..5974)
                     /gene="rhtB"
                     /locus_tag="ACHX5T_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR8466452.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6085..7107
                     /gene="pldB"
                     /locus_tag="ACHX5T_001180"
     CDS             6085..7107
                     /gene="pldB"
                     /locus_tag="ACHX5T_001180"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR8466453.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7115..7915
                     /gene="yigL"
                     /locus_tag="ACHX5T_001181"
     CDS             7115..7915
                     /gene="yigL"
                     /locus_tag="ACHX5T_001181"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR8466454.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7991..8890
                     /gene="bioP"
                     /locus_tag="ACHX5T_001182"
     CDS             7991..8890
                     /gene="bioP"
                     /locus_tag="ACHX5T_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR8466455.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8778..9731)
                     /gene="metR"
                     /locus_tag="ACHX5T_001183"
     CDS             complement(8778..9731)
                     /gene="metR"
                     /locus_tag="ACHX5T_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR8466456.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9967..12228
                     /gene="metE"
                     /locus_tag="ACHX5T_001184"
     CDS             9967..12228
                     /gene="metE"
                     /locus_tag="ACHX5T_001184"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR8466457.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12267..13082)
                     /locus_tag="ACHX5T_001185"
     CDS             complement(12267..13082)
                     /locus_tag="ACHX5T_001185"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR8466458.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13344..14105
                     /gene="udp"
                     /locus_tag="ACHX5T_001186"
     CDS             13344..14105
                     /gene="udp"
                     /locus_tag="ACHX5T_001186"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR8466459.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            14246..15673
                     /gene="rmuC"
                     /locus_tag="ACHX5T_001187"
     CDS             14246..15673
                     /gene="rmuC"
                     /locus_tag="ACHX5T_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR8466460.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15768..16523
                     /gene="ubiE"
                     /locus_tag="ACHX5T_001188"
     CDS             15768..16523
                     /gene="ubiE"
                     /locus_tag="ACHX5T_001188"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR8466461.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16537..17142
                     /gene="ubiJ"
                     /locus_tag="ACHX5T_001189"
     CDS             16537..17142
                     /gene="ubiJ"
                     /locus_tag="ACHX5T_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR8466462.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17139..18779
                     /gene="ubiB"
                     /locus_tag="ACHX5T_001190"
     CDS             17139..18779
                     /gene="ubiB"
                     /locus_tag="ACHX5T_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR8466463.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18858..19127
                     /gene="tatA"
                     /locus_tag="ACHX5T_001191"
     CDS             18858..19127
                     /gene="tatA"
                     /locus_tag="ACHX5T_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR8466464.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19131..19646
                     /gene="tatB"
                     /locus_tag="ACHX5T_001192"
     CDS             19131..19646
                     /gene="tatB"
                     /locus_tag="ACHX5T_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR8466465.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19649..20425
                     /gene="tatC"
                     /locus_tag="ACHX5T_001193"
     CDS             19649..20425
                     /gene="tatC"
                     /locus_tag="ACHX5T_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR8466466.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20467..21249
                     /gene="tatD"
                     /locus_tag="ACHX5T_001194"
     CDS             20467..21249
                     /gene="tatD"
                     /locus_tag="ACHX5T_001194"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR8466467.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21246..21734)
                     /gene="rfaH"
                     /locus_tag="ACHX5T_001195"
     CDS             complement(21246..21734)
                     /gene="rfaH"
                     /locus_tag="ACHX5T_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR8466468.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21901..23394
                     /gene="ubiD"
                     /locus_tag="ACHX5T_001196"
     CDS             21901..23394
                     /gene="ubiD"
                     /locus_tag="ACHX5T_001196"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR8466469.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23440..24141
                     /gene="fre"
                     /locus_tag="ACHX5T_001197"
     CDS             23440..24141
                     /gene="fre"
                     /locus_tag="ACHX5T_001197"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR8466470.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24333..25496)
                     /gene="fadA"
                     /locus_tag="ACHX5T_001198"
     CDS             complement(24333..25496)
                     /gene="fadA"
                     /locus_tag="ACHX5T_001198"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR8466471.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25506..27695)
                     /gene="fadB"
                     /locus_tag="ACHX5T_001199"
     CDS             complement(25506..27695)
                     /gene="fadB"
                     /locus_tag="ACHX5T_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR8466472.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27885..29216
                     /gene="pepQ"
                     /locus_tag="ACHX5T_001200"
     CDS             27885..29216
                     /gene="pepQ"
                     /locus_tag="ACHX5T_001200"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR8466473.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29216..29830
                     /locus_tag="ACHX5T_001201"
     CDS             29216..29830
                     /locus_tag="ACHX5T_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR8466474.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29869..31320
                     /gene="trkH"
                     /locus_tag="ACHX5T_001202"
     CDS             29869..31320
                     /gene="trkH"
                     /locus_tag="ACHX5T_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR8466475.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31332..31877
                     /gene="hemG"
                     /locus_tag="ACHX5T_001203"
     CDS             31332..31877
                     /gene="hemG"
                     /locus_tag="ACHX5T_001203"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR8466476.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7482 a         8149 c         8757 g         7698 t
ORIGIN      
        1 aaaaaagata ataagaaagg caaaaatgaa atttattaaa ataaataaaa atatatatcg
       61 gtgtctggaa atcttaccgt tagatgttgg gataaacata cgtaacataa attttacatc
      121 cttgtgtgag tctcctgtca gcatggcaat atgcccactc tcatacaaga gtcggcatat
      181 tttttcagaa tatatttatt ttttatttgg acgttctacg ctgcgtataa atcgcgtcca
      241 tcacaaaaat tgccagcgcc acccaaataa aggtgaaagt caccatctta tcggcacccg
      301 gtttttcacc ataaaacgtc acagccagca ggaacatcag cgtcgggcca atgtactgaa
      361 aaaagcctaa cgttgagaga cgcaggcgcg tggcagcggc agtaaaacac aacagcggta
      421 cggtagtgac aataccggcg gcgatcagca gtaaattcag cgacatcggg ttttgcccca
      481 tatggctggt tgaactgtcg gcaatagcaa acaggtaaat cgccgccacg ggcagcagcc
      541 acatggtttc aattaacatg ccggtttgtg cttctaccgc aatcttcttg cgcaccaggc
      601 cgtagaaggc aaaactaaat gccagcccca gcgcgataat aggtagcgaa ccaaaagtcc
      661 acagctggac caacacgcca catatcgcca gaatcaccgc cagccattgc atccggcgga
      721 atcgctcgcc gaggaaaatc atccccagca caatgttcac cagcgggtta ataaagtaac
      781 caaggctcgc ttccagcata tggtgattgt tcaccgccca gataaacagt agccagttgc
      841 caccaatcag tacggcagag actgccagca taaaaatttt ctgtggcgtc tgaatcagcg
      901 tttttaaata ggaccactgg cggcaaatgc tcatcagcac caccataaag aaaaacgacc
      961 agatcacgcg atgcgtcagg atctcatcgg cgggcacgta gtaaatcagc ttgaagtatg
     1021 ctggcgcaat accccaaata aaataagcgg caagagcgag taatacgccc tgccgcgttt
     1081 gttttgcatc catcgggaat actcattttt aattggtaac agcagtttac ctgcttttat
     1141 gtcttcaacc taccatatag gtggcggtgg cactggcaat ataaagctgt tcttcattgt
     1201 gtaattcaac gcgggcgacg gcgactttat tgcctgcacg caacaggcta ctggtagcag
     1261 taaaacgctc gcccctgcct gggcgcagat aatcaacgcg aagatcaatg gtccccatcc
     1321 gcgatagccg ctggcgtagt tcatcttcac tgatggtttc gtggcgggtt aaggtacttc
     1381 ccacgcacac cagaccggcg gcgacatcca gcgccgacgc aatgactccg ccgtgcaaaa
     1441 tgctttgcgc ccagttaccc accatcatcg gctgattttt aaaggccagc tgtgcgaact
     1501 ctttttcgta acgctccagt tccatcccca atgcgcggtt aaatggcatg tgataaacaa
     1561 acatctcacc cactaatttc agggcttgtt cagcggtcag tacggcagac atatcatcct
     1621 tacacttcat tggttaatga aatgttgatt ttatgcttct ttgttgttgg tttctacttt
     1681 aggaagggat aactaacggc aatggagtta agtatgtaaa atagcccgca gaaaaacatt
     1741 caccttatca ataattcgtt acggagaaca cgaccgatgc ggactctgca gggctggttg
     1801 ttgccggtgt ttatgttgcc tatggcagta tatgcacaag aggcaacggt gaaagaggtg
     1861 catgacgcgc cagcggtgcg tggcagtatt atcgccaata tgctgcagga gcatgacaat
     1921 ccgttcacgc tctatcctta tgacaccaac tacctcatct acacccaaac cagcgatctg
     1981 aataaagaag cgattgccag ttacgactgg gcggaaaatg cgcgtaagga tgaagtaaag
     2041 tttcagttga gcctggcatt tccgctgtgg cgtgggattt taggcccaaa ctcggtgttg
     2101 ggtgcgtctt atacgcaaaa atcctggtgg caactgtcca atagcgaaga gtcttcaccg
     2161 tttcgtgaaa ccaactacga accgcaattg ttcctcggtt ttgccaccga ttaccgtttt
     2221 gcaggttgga cgctgcgcga tgtggagatg gggtataacc acgactctaa cgggcgttca
     2281 gacccgacct cccgcagctg gaaccgcctt tatactcgcc tgatggcaga aaacggtaac
     2341 tggctggtag aagtgaagcc gtggtatgtg gtgggtaata ctgacgataa cccggatatc
     2401 accaaatata tgggttacta ccagcttaaa atcggctatc acctcggtga tgcggtgctc
     2461 agtgcgaaag gacagtacaa ctggaacacc ggctacggcg gcgcggagtt aggcttaagt
     2521 tacccgatca ccaaacatgt gcgcctttat actcaggttt acagcggcta tggcgaatcg
     2581 ctcatcgact ataacttcaa ccagacccgt gtcggtgtag gggttatgct aaacgacttg
     2641 ttttgatgaa cggttgagtg gttggcaaat ctggaatcca gcatccagga ttaccctctc
     2701 agagactaaa agcattgcag tttctcgcgc aggcgctgaa aatagcgcct gtttttattt
     2761 caggcaatcg gggtgaatgt ggcgcaggcg gaagtgttga atctggagtc cggagctaaa
     2821 caggttttac aagaaacctt tggctaccaa cagtttcgcc ccggccagga agaaattatc
     2881 gacactgtgc tttccggtcg cgattgcctg gttgttatgc ccactggtgg cggaaaatcc
     2941 ctttgctatc aaattcctgc cttattgcta aacggcctta ccgtggttgt ttcaccgctg
     3001 atttcgttga tgaaagatca ggtggatcaa ctacaggcca acggcgtggc ggcggcgtgc
     3061 cttaactcga cgcaaacccg cgaacagcaa cttgaagtga tgacaggctg ccgcaccggg
     3121 caaattcgct tactgtatat cgccccggaa cgcctgatgc tggataactt tcttgagcat
     3181 ctggcgcact ggaatccggt gttattagcc gtcgatgaag cgcactgtat ctcccaatgg
     3241 ggtcacgatt tccgcccgga atatgccgcg ctcggtcagt tgcgccagcg gttcccgacg
     3301 ctgccgttta tggcgctgac cgccacagcc gacgacacca cgcgccagga tatcgtgcgc
     3361 ctgctggggc tgaacgatcc gctgattcaa atcagcagtt ttgaccgtcc gaatattcgc
     3421 tacatgctga tggagaaatt caaaccgctc gatcagttga tgcgctacgt gcaggaacag
     3481 cgcggtaagt ctggcattat ctactgcaac agccgggcga aagtagaaga caccgctgcg
     3541 cgcctgcaaa gcaggggtat tagcgcggcg gcctatcatg ccgggctgga aaataatgtc
     3601 cgcgccgacg tgcaggagaa attccagcgc gatgatctgc aaattgtggt ggcgacggtg
     3661 gcgttcggca tgggcatcaa taaaccaaac gttcgcttcg tggtccactt tgatattccg
     3721 cgcaatatcg aatcctatta tcaggaaacc ggtcgcgccg ggcgtgatgg cctgcctgcg
     3781 gaagcgatgc tgttttacga tccggctgat atggcgtggc tgcgccgttg tctggaagag
     3841 aagccgcagg ggcagttgca ggatatcgag cgccacaaac tcaatgcgat gggcgcgttt
     3901 gccgaagcgc aaacttgccg tcgtctggta ttgctgaact attttggcga agggcgtcag
     3961 gagccgtgcg ggaactgcga tatctgcctc gatccgccga aacagtacga cggttcaacc
     4021 gatgctcaga ttgccctttc caccattggt cgtgtgaatc agcggtttgg gatgggttat
     4081 gtggtggaag tgattcgtgg tgctaataac cagcgtatcc gcgactatgg tcatgacaaa
     4141 ctgaaagtct atggtatggg ccgtgataaa agccatgaac attgggtgag cgtgatccgc
     4201 cagctgattc atctcggcct ggtgacgcaa aatattgccc agcattctgc cctacaactg
     4261 acagaggccg cgcgcccggt gctgcgcggc gaatcctctt tgcaacttgc cgtgccgcgt
     4321 atcgtggcgc tcaaaccgaa agcgatgcag aaatcgttcg gcggcaacta tgatcgcaaa
     4381 ctgttcgcca aattacgcaa actgcgtaaa tcgattgccg atgaaagtaa tgtcccgccg
     4441 tacgtggtgt ttaacgacgc aaccttgatt gagatggctg aacagatgcc gatcaccgcc
     4501 agcgaaatgc tcagcgttaa cggcgttggg atgcgcaagc tggaacgctt tggtaaaccg
     4561 tttatggcgc tgattcgtgc gcatgtcgat ggcgacgacg aagagtagtc agcagcataa
     4621 aaaagtgcca gtatgaagac tccgtaaacg tttcccccgc gagtcaaatg tatgttgatg
     4681 ttatttctca ccgtcgccat ggtgcacatt gtggcgctta tgagccccgg tcccgatttc
     4741 ttttttgtct ctcagaccgc tgtcagtcgt tcccgtaaag aagcgatgat gggcgtgctg
     4801 ggcattacct gcggcgtaat ggtttgggct gggattgcgt tgcttggcct gcatttgatt
     4861 atcgaaaaaa tggcctggct gcatacgctg attatggtag gcggtggcct gtatctctgc
     4921 tggatgggtt accagatgct acgcggtgca ctgaaaaaag aggctgtatc tgcgcctgca
     4981 ccacaggttg agctggcgaa aagcgggcgc agtttcctga aaggcttact gaccaatctc
     5041 gccaatccga aagcgattat ctattttggt tcggtattct cattgtttgt cggcgataac
     5101 gttggcacta ccgagcgctg gggcattttc gcgttgatta ttgtcgaaac actggcgtgg
     5161 tttaccgtcg ttgccagtct gtttgccctg ccgcaaatgc gccgtggtta tcaacgtctg
     5221 gcgaagtgga ttgatggttt tgccggggcg ttatttgccg gatttggcat tcatttgatt
     5281 atttcgcggt gatgccagac gcgtcttcag agtaagtcgg ataaggcgtt tacgccgcat
     5341 ccgacattat ttttcacgca tgcctcgccg atgctaacag cgctcccacc agcataaaca
     5401 acgagccgaa aatcttattc agcgccttca tctgctttgg tcctttaatc catagagcaa
     5461 tcctttgagc aagggtggcg taaccgatca tcacaataat atcgaccaca atagtggtga
     5521 cgccgagcac gatatactgc atcagttgcg gctgttgcgg catgatgaat tgcggaaata
     5581 gcgccgccag aaacacaata cttttgggat tggtgagatt cacaaaaact gcgcgctgga
     5641 acaaatgtcg acgcgattga gtagaggcca gcgatttaag atcaattgcg ccagcggcgc
     5701 gccactgctg gattcccagc caaatcaagt aagccgcgcc tgcccacttt aacacttcaa
     5761 acgcaatcac tgagcgggaa aatagcgtcc ccaaccccac gccaaccagc acaatatgaa
     5821 tcgccagtcc ggtctgaagc ccagcaatag acgccaccgc gccgcgataa ccgtggttga
     5881 gcgaggtggt catagtgttg attgcaccag agcctggcga caggcttaaa atgatcgatg
     5941 tcagcaggta ggcaaaccac cattctaagg tcatgatgaa ctcccggtgt gtctattttt
     6001 gtgccacaat acgctactgt cgcagcgttg tgtcaggcac gcttaaaaaa acgattttac
     6061 gtggtttaag aggcagatta cccgatgttt cagcagcaaa aagactggga aacaagagaa
     6121 aacgcgtttg ctgcttttac catgggaccg ctgactgatt tctggcgtca gcgtgatgaa
     6181 gcagagttta ctggtgtgga tgacattccg gtgcgctttg tccgttttcg cgcacagcac
     6241 catgaccggg tggtagtcat ctgcccgggg cgtattgaga gctacgtaaa atatgcggaa
     6301 ctggcctacg acctgttcca tttggggttt gatgtcttaa tcatcgacca tcgcgggcag
     6361 ggacgttccg gtcgcctgtt agccgatccg catctcgggc atgttaatcg ctttaatgat
     6421 tatgttgatg atctggcggc attctggcag caggaggttc agcccggtcc gtggcgtaaa
     6481 cgctatatac tggcacattc gatgggcggt gcgatctcca cattatttct gcaacgccat
     6541 ccaggtgtat gtgacgccat tgcgctaact gcgccaatgt ttgggatcgt gattcgtatg
     6601 ccgtcattta tggcacggca gatcctcaac tgggccgaag cgcatccacg tttccgtgat
     6661 ggctatgcaa taggcaccgg gcgctggcgc gcgttgccgt ttgctatcaa cgtactgacc
     6721 cacagcagac agcgatatcg acgtaactta cgcttctatg ctgatgaccc aacgattcgc
     6781 gtcggtgggc cgacctacca ttgggtacgc gaaagtattc tggctggcga acaggtgtta
     6841 gccggtgcgg gtgatgacgc cacgcaaacg cttctcttac aagctgaaga ggaacgcgtg
     6901 gtggataacc gcatgcacga ccgtttttgt gaactccgca ccgccgcggg ccatcctgtc
     6961 gaaggaggac ggccgttggt aataaaaggt gcttaccatg agatcctttt tgaaaaggac
     7021 gcaatgcgct cagtcgcgct ccacgccatc gttgattttt tcaacaggca taactcaccc
     7081 agcggaaacc gctctacaga ggtttaaatt tcttatgtac caggttgttg cgtctgattt
     7141 agatggcacg ttactttctc ccgaccatac gttatcccct tacgccaaag aaactctgaa
     7201 gctgctcacc gcgcgcggca tcaactttgt gtttgcgacc ggtcgtcacc acgttgatgt
     7261 ggggcaaatt cgcgataatc tggagattaa gtcttacatg attacctcca atggtgcgcg
     7321 cgttcacgat ctggatggca atctgatttt tgctcataac ctggatcgcg acattgccag
     7381 cgatctgttt ggcgtagtca acgacaatcc ggacatcatt actaacgttt atcgcgacga
     7441 cgaatggttt atgaatcgcc atcgcccgga agagatgcgc ttttttaaag aagcggtgtt
     7501 caaatatgcg ctgtatgagc cgggattact ggagccggaa ggcatcagca aagtgttttt
     7561 cacctgcgat tcccatgaac aactgctgcc gctggagcag gcgattaacg ctcgctgggg
     7621 cgatcgcgtc aacgtcagtt tctctacctt aacctgtctg gaagtgatgg cgggcggcgt
     7681 gtcgaaaggc catgcgctgg aagcagtggc gaagaaactg ggctacagcc tgaaggattg
     7741 tattgcgttt ggtgacggga tgaacgacgc cgaaatgctg tcgatggcgg ggaaaggctg
     7801 cattatgggc agtgcgcacc agcgtctgaa agatctgcat ccggagctgg aagttattgg
     7861 tactaatgcc gacgacgcgg tgccgcatta tctgcgtaaa ctctatttat cgtaatcgtt
     7921 ctttatttgg tcagttgtca acctgatact tcgctacaat ggatactcgt taatcaaaga
     7981 gttttccatt gtggcgttac ttatcatcac cacgattctg tgggccttct cctttagctt
     8041 ttatggcgag taccttgcgg ggcacgtcga tagctatttt gcggtgctgg tgcgcgttgg
     8101 cttggcggca ctcgtttttc tgccgtttct gcgtacccgt agcaatagcc tgaaaacggt
     8161 cggcctgtat atgctggtgg gcgcgatgca gctcggcgtg atgtatatgc tgagtttccg
     8221 cgcttatctc tacctgacgg tctccgaatt gttgctgttc accgtactga cgccgctcta
     8281 catcacgctg atttatgaca tcatgagtaa gcgccgtctg cgctggggct atgcttttag
     8341 cgccttgctg gcggtgattg gtgccgggat tattcgctat gatcaggtca ccgaccattt
     8401 ctggactggc ttgttgctgg tgcaactctc caatatcact tttgccatcg gcatggtggg
     8461 ttacaaacgc ctgatggaaa ctcgcccgat gccgcagtat aacgcctttg cgtggttcta
     8521 tcttggcgcg ttcctggtgg cggtaattgc atggttcttg ctgggaaatg cgcagaaaat
     8581 gccgcaaacc acgttgcaat ggggcattct ggtgtttctt ggcgtggtgg cttccgggat
     8641 tggctacttt atgtggaact acggcgcgac gcaggtggac gccggaacgc tgggcattat
     8701 gaataatatg cacgttccgg cagggctgct ggtaaacctg gctatctggc accaacagcc
     8761 gcactggcca acgtttatta caggcgcgct ggtgatcctg gcctcactgt gggtgcatcg
     8821 taagtgggtc gctccgcgct cttcacaaac ggcagatgat cgcaggcgtg attgcgcgct
     8881 gagcgaataa acgcttccgt aactggctga cgctgctcgc catcgcgcac ggcggcgtac
     8941 agtcggctcc acaagccttc gcccagggtt ttggtcacca ccagaccctg gcgctcaaaa
     9001 ctctctacta cccaatgcgg tagcgcggca atacccatcc gcgcggcaac catttgaatc
     9061 aacaataagg tgttatcgac gcttttcagt gacgggctga cgcctgccgg ctgaagaaaa
     9121 tgccgccaga catccagtcg actacgctgc accggataaa ttaatagcgt ctcgctggcg
     9181 agatcttccg gtgtaatacg cgtttttgcc gccagtggat ggtcaggtgc taacaccagg
     9241 cgtacttcat aatcgaacat cggcgaataa tgcaggccac tgcgcggcag gatatcggag
     9301 gtcatcacca gatccagctc gccctgttgc aacgcgggct gcgggtcaaa cgtcacgccc
     9361 gatttaaaat ccatctctac ctgcggccag ttcttatgga aatttcctaa agcgggtgtc
     9421 agccactgaa tacagctatg gcactcaatg gcaatgcgca gacgcgtctg ctgcggttca
     9481 ttgcaggctt gcagtgcctg gctgatttgc ggcagcacct ggtttgccag ttgcaacagg
     9541 atttctccct gcggtgtaaa gcgtagcggc tggctcttac gcacaaatag ccggaagcca
     9601 aggcgttgtt ccagatcgct aaactggtga gacagggcgg attgcgtctg atgcaacgtt
     9661 gccgcagcgg ctgcgagcga gccgcagttc cgcaacgctt gtagcgtttt caggtgtttt
     9721 acttcgatca tgaaagtcct tcacttcggc atgaataatt tgcgcttgag gaatatacag
     9781 taaccgccaa ttatggatgt gtaaacatct ggacggctaa aatcattcgt cttttaaatt
     9841 tatggtgcgt tggctgcgtt tctccacccc ggtcacttac ttcagtaagc tcccggcgat
     9901 gaataaactt gccgccttcc ctaaattcaa aatccatagg atttacatat aattagagga
     9961 agaaaaatga caatattgaa tcacaccctc ggtttccctc gcgttggcct gcgtcgcgag
    10021 ctgaaaaaag cgcaagaaag ttattgggcg gggaactcca cgcgtgaaga actgctgaca
    10081 gtagggcgtg aactgcgtgc tcgtcactgg gatcaacaaa agcaagcggg tatcgacctg
    10141 ctgccggtgg gcgattttgc ctggtacgat catgtactga ccaccagttt gctgctgggt
    10201 aatgttccgc cacgtcatca gaacaaagac ggttcggtag atatcgacac cctgttccgt
    10261 attggtcgtg gacgtgcgcc gactggcgaa cctgcggcgg cagcggaaat gaccaaatgg
    10321 tttaacacca actatcacta catggtgccg gagttcgtta aaggccaaca gttcaaactg
    10381 acttggacgc agctgctgga ggaagtggac gaggcgctgg cgctgagcca caacgttaag
    10441 cctgtgctgc tggggccggt tacttacctg tggctgggga aagtgaaagg tgaacagttt
    10501 gaccgcctta gcctgctgaa cgacattctg ccggtttatc agcaagtgct ggcagaactg
    10561 gcgaaacgcg gcatcgagtg ggtacagatt gatgaacccg cgctggtact ggaactacca
    10621 caggcgtggc tggacgcata caaacccgct tacgacgcgc tccagggaca ggtgaaactg
    10681 ctgctgacca cctattttga aggcgtaacg ccaaatctcg acacgattac tgcgctgcct
    10741 gttcaggggc tgcatgtgga ccttgtacat ggtaaagata acgttgttga actgcacaag
    10801 cgcctgcctt ctgactggct gctgtctgcg ggtctgatca atggtcgtaa cgtctggcgc
    10861 gccgatctta ctgagaaata tgcgcaaatt aaggacattg tcggcaaacg tgatttgtgg
    10921 gtggcatctt cctgctcgtt gctgcacagc cccatcgacc tgagcgtgga aacgcgtctt
    10981 gatgcagaag tgaaaagctg gtttgccttc gccctacaaa aatgccatga actggcactg
    11041 ctgcgcgatg cgctgaacag tggtgacacg gcagctctgg cagagtggag cgccccaatt
    11101 caggcgcgtc gtcactctac tcgtgtacat aatccggcgg tagaaaagcg tctggcggcg
    11161 atcaccgccc aggacagcca gcgtgcgaat gtctatgaag tgcgtgctga agcccagcgt
    11221 gcgcgtttta aactgccagc gtggccgacc accacgattg gttccttccc gcaaaccacg
    11281 gaaattcgta ccctgcgtct ggatttcaaa aagggcaatc tcgacgccaa caactaccgc
    11341 acgggcattg cggaacatat caagcaggcc attgttgagc aggaacgttt gggactggat
    11401 gtgctggtac atggcgaggc cgagcgtaat gacatggtgg aatactttgg cgagcacctc
    11461 gacggatttg tctttacgca aaacggttgg gtacagagct acggttcccg ctgcgtgaag
    11521 ccaccgattg tcattggtga cattagccgc ccggcaccga ttaccgtgga gtgggcgaag
    11581 tatgcgcaat cgctgaccga caaaccggtg aaagggatgc tgacggggcc ggtgaccata
    11641 ctctgctggt cgttcccgcg tgaagatgtc agccgtgaaa ccatcgccaa acagattgcg
    11701 ctggcgctgc gtgatgaagt ggccgatctg gaagccgctg gaattggcat catccagatt
    11761 gacgaaccgg cgctgcgcga aggtttaccg ctgcgtcgta gcgactggga tgcgtatctc
    11821 cagtggggcg tagaggcctt ccgtatcaac gccgccgtgg cgaaagatga cacacaaatc
    11881 cacactcaca tgtgttattg cgagttcaac gacatcatgg attcgattgc ggcgctggac
    11941 gcagacgtca tcaccatcga aacctcgcgt tccgacatgg agttgctgga gtcgtttgaa
    12001 gagtttgatt atccaaatga aatcggtcct ggcgtctatg acattcactc gccaaacgta
    12061 ccgagcgtgg aatggattga agccttgctg aagaaagcgg caaaacgcat tccggcagag
    12121 cgcctgtggg tcaacccgga ctgtggcctg aaaacgcgcg gctggccaga aacccgcgcg
    12181 gcactggcga acatggtgca ggcggcgcag aatttgcgtc gggggtaaat ccaaaccggg
    12241 tggtaatacc acccggtctt ttctcattac agcgacttct tcccaccata ctgcttaaac
    12301 cattccagca tacgctgcca gccatctttc gcagattcgg catgatagct cgggcgataa
    12361 tcagcgttga atgcatgccc ggcatccggg tacacgataa tctctgcttt cgcattagca
    12421 gcccgcagcg cctggcgcat cgtttcaacg ctctcctgcg gaatgctgtt atcctgacca
    12481 ccatataagc cgagaaccgg cgcgttaaga tcggttgcga tatcaacagg ttgtttcggt
    12541 gaattcagcg acttgtcgcc cgtcagtttg ccgtaccacg ccactgcggc ttttagctgt
    12601 ggattatgcg cggcatacag ccaggtgata cgtccacccc agcagaatcc ggtgatcatt
    12661 aaacgatgaa catcgccgcc gttgcgggaa gcccaactgg cgacatgatc gagatcggcc
    12721 agcacctgcg agtcaggcac ttttgctacc agaccgctaa gcaacgtggg gatatcggca
    12781 aaatcattcg gatcgccttc gcggaagtaa agttcaggtg cgatagccag atacccctcc
    12841 agcgccagac ggcgacaaat gtcgcggata tgttcatgca cgccaaaaat ttcctgcact
    12901 acaatgacca ctggcagtgg gccatcgctt tgctttggtc tggcatggta agcaggcatg
    12961 ttatcccctt gtgaagggat agaggtgaag cccgccacaa tcgcgtcgtc cggggtctga
    13021 acgatggtcg aagcgagagg cgatgcagca ggtgcaaatc cagattgttg tgttgttgcc
    13081 atggtattct ccgtaccctt ataaaaatgt tgcgcaatgt taactatagt cagcatgcaa
    13141 caaatcacat tgcctgaatc ggctcatctt ttatgcagtc ctgcagaatg aagagtgatt
    13201 tatgtgatat gaatcacttt tggtgggtaa atttatgcaa cgcatttgcg tcatggtgat
    13261 gagtatcacg aaaaaatgtt aaacccttcg gtaaagtgtc tttttgcttc ttctgactaa
    13321 accgattcac agaggagttg tatatgtcca agtctgatgt ttttcatctc ggcctcacta
    13381 aaaacgattt acaaggggct acgcttgcca tcgtccctgg cgacccggat cgtgtggaaa
    13441 agatcgccgc gctgatggat aagccggtta agctggcatc tcaccgcgaa ttcactacct
    13501 ggcgtgcaga gctggatggt aaacctgtta tcgtctgctc taccggtatc ggcggcccgt
    13561 ctacctctat tgctgttgaa gagctggcac agctgggcat tcgcaccttc ctgcgtatcg
    13621 gtacaacggg cgctattcag ccgcatatta atgtgggtga tgtcctggtt accacggcgt
    13681 ctgtccgtct ggatggcgcg agcctgcact tcgcaccgct ggaattcccg gctgtcgctg
    13741 atttcgaatg tacgactgcg ctggttgaag ctgcgaaatc tattggcgcg acaactcacg
    13801 ttggcgtgac agtttcttct gataccttct acccaggtca ggaacgttac gatacttact
    13861 ctggtcgcgt agttcgtcac tttaaaggtt ctatggaaga gtggcaggcg atgggcgtaa
    13921 tgaactatga aatggaatct gcaactctgc tgaccatgtg tgcaagtcag ggcctgcgtg
    13981 ccggtatggt agcgggtgtt atcgttaacc gcacccagca agagatcccg aatgctgaga
    14041 cgatgaaaca aaccgaaagc catgcggtga aaatcgtggt ggaagcggcg cgtcgtctgc
    14101 tgtaattctc ttctcctgtc tgaaggccga cgcgttcggc cttttgtatt tttgcgtagc
    14161 gcctcgcagg aaatgccttt ccaactggac gtttgtacag cacaattcta ttttgtgcgg
    14221 gtaagttgtt gcgtcaggag gcgttgtgga tttctcaatc atggtttacg cagttattgc
    14281 gttggtgggt gtggcaattg gctggctgtt tgccagttat caacatgcgc agcaaaaagc
    14341 cgagcaatta gctgaacgtg aagagatggt cgcggagtta agcgcggcaa aacaacaaat
    14401 tacccaaagc gagcactggc gtgcagagtg tgaattgctc aataacgaag tgcgcagcct
    14461 gcaaagtatt aacacctctc tggaggccga tctgcgtgaa gtaaccacgc ggatggaagc
    14521 cgcacagcaa catgctgacg ataaaattcg ccagatgatt aacagcgagc agcgcctcag
    14581 tgagcagttt gaaaacctcg ccaaccgtat ttttgagcac agcaatcgcc gggttgatga
    14641 gcaaaaccgt cagagtctga acagcctgtt gtcgccgcta cgtgaacaac tggacggttt
    14701 ccgccgtcag gttcaggaca gcttcggtaa agaagcacaa gaacgccata ccctgaccca
    14761 cgaaattcgc aatctccagc aactcaacgc gcaaatggcc caggaagcga tcaacctgac
    14821 gcgcgcgctg aaaggcgaca ataaaaccca gggcaactgg ggcgaggtag tattgacgcg
    14881 ggtgctggag gcttccggtc tgcgtgaagg gtatgaatat gaaacccagg tcagcatcga
    14941 aaatgacgcc cgctcgcgga tgcagccgga tgtcatcgtg cggctgccgc agggaaaaga
    15001 tgtggtgatc gacgccaaaa tgacgctggt cgcctatgaa cgctatttta acgccgaaga
    15061 cgactacacc cgcgaaagcg cgctacagga acatatcgcg tcggtgcgta accatatccg
    15121 tttgctggga cgcaaagatt atcaacagct gccggggctg cgaactctgg attacgtgct
    15181 gatgtttatt cccgttgaac ccgctttttt actggcgctt gaccgccagc cggagctgat
    15241 caccgaagcg ttgaaaaaca atatcatgct ggttagcccg actacgctgc tggtggcgtt
    15301 gcgtactatt gccaacctgt ggcgttatga gcatcaaagc cgtaacgccc agcaaatcgc
    15361 cgatcgtgcc agcaagctgt acgacaagat gcgtttgttc atcgatgaca tgtccgcgat
    15421 tggtcaaagt ctcgacaaag cgcaggataa ctaccgccag gcaatgaaaa aactctcttc
    15481 aggtcgcgga aatgtgctgg cgcaggcaga agcgtttcgc ggtttaggag tagaaattaa
    15541 acgcgagatt aatccggatt tggctgaaca ggcggtgagc caggatgaag agtatcgact
    15601 tcggtcggtt ccggagcagc cgaatgatga agcttatcaa cgcgatgatg aatataatca
    15661 gcagtcgcgc tagcccatta ggggtagtta agccgggtag aaatctaggg catcgacgcc
    15721 caatctgtta cacttctgga acaatttttt gatgagcagg cattgagatg gtggataagt
    15781 cacaagaaac gacgcacttt ggttttcaga ccgtcgcgaa ggaacaaaaa gcggatatgg
    15841 tcgcccacgt tttccattcc gtggcatcaa aatacgatgt catgaatgat ttgatgtcat
    15901 ttggtattca tcgtttgtgg aagcgattca cgattgattg cagcggcgta cgccgtgggc
    15961 agaccgtgct ggatctggct ggtggcaccg gcgacctgac agcgaaattc tcccgcctgg
    16021 tcggagaaac tggcaaagtg gtccttgctg atatcaatga atccatgctc aaaatgggac
    16081 gcgagaagct gcgtaatatc ggtgtgattg gcaacgttga gtatgttcag gcgaacgctg
    16141 aggcgctgcc gttcccggat aacacctttg attgcatcac catttcgttt ggtctgcgta
    16201 acgtcaccga caaagataaa gcactgcgtt caatgtatcg cgtgctgaaa cccggcggcc
    16261 gcctgctggt gcttgagttc tcgaagccaa ttatcgagcc gctgagcaaa gcctatgatg
    16321 catactcctt ccatgtgctg ccgcgtattg gctcactggt cgcgaacgac gccgacagct
    16381 accgttatct ggcagaatcc atccgtatgc atcccgatca ggataccctg aaagccatga
    16441 tgcaggatgc cggattcgaa agtgtcgact actacaatct gacggcaggg gttgtggcgc
    16501 tgcatcgtgg ttataagttc tgacaggaga ccggaaatgc cttttaaacc tttagtgacg
    16561 gcaggaattg aaagtctgct caacaccttc ctgtatcgct cacccgcgct gaaaacagcc
    16621 cgctcgcgtc tgctgggtaa agtattgcgc gtggaggtaa aaggcttttc gacgtcattg
    16681 attctggtgt tcagcgaacg ccaggttgat gtactgggcg aatgggcagg cgatgctgac
    16741 tgcaccgtta tcgcctacgc cagtgtgttg ccgaaacttc gcgatcgcca gcagcttacc
    16801 gcactgattc gcagtggtga gctggaagtg cagggcgata ttcaggtggt gcaaaacttc
    16861 gttgcgctgg cagatctggc agagttcgac cctgcggaac tgctggcccc ttataccggt
    16921 gatatcgccg ctgaaggaat cagcaaagcc atgcgcggag gcgcaaagtt cctgcatcac
    16981 ggcattaagc gccagcaacg ttatgtggcg gaagccatta ctgaagagtg gcgtatggca
    17041 cccggtccgc ttgaagtggc ctggtttgcg gaagagacgg ctgccgtcga gcgtgctgtt
    17101 gatgccctga ccaaacggct ggaaaaactg gaggctaaat gacgccaggt gaagtacggc
    17161 gcctatattt catcattcgc acttttttaa gctacggact tgatgaactg atccccaaaa
    17221 tgcgtatcac cctgccgcta cggctatggc gatactcatt attctggatg ccaaatcggc
    17281 ataaagacaa acttttaggt gagcgactac gactggccct gcaagaactg gggccggttt
    17341 ggatcaagtt cgggcaaatg ttatcaaccc gccgcgatct ttttccaccg catattgccg
    17401 atcagctggc gttattgcag gacaaagttg ctccgtttga tggcaagctg gcgaagcagc
    17461 agattgaagc tgcaatgggc ggcttgccgg tagaagcgtg gtttgacgat tttgaaatca
    17521 agccgctggc ttctgcttct atcgcccagg ttcataccgc gcgattgaaa tcgaatggta
    17581 aagaggtggt gattaaagtc atccgcccgg atattttgcc ggtcattaaa gcggatctga
    17641 aacttatcta ccgtctggct cgctgggtgc cgcgtttgct gccggatggt cgccgtctgc
    17701 gcccaaccga agtggtgcgc gagtacgaaa agaccttgat tgatgaactg aatttgctgc
    17761 gggaatctgc caacgccatt cagcttcggc gcaattttga agacagcccg atgctctaca
    17821 tcccggaagt ttaccctgac tattgtagtg aagggatgat ggtgatggag cgcatttacg
    17881 gcattccggt gtctgatgtt gcggcgctgg agaaaaacgg cactaacatg aaattgctgg
    17941 cggaacgcgg cgtgcaggtg ttcttcactc aggtctttcg cgacagcttt ttccatgccg
    18001 atatgcaccc tggcaacatc ttcgtaagct atgaacaccc ggaaaacccg aaatatatcg
    18061 gcattgattg cgggattgtt ggctcgctaa acaaagaaga taaacgctat ctggcagaaa
    18121 actttatcgc cttctttaat cgcgactatc gcaaagtggc agagctacac gtcgattctg
    18181 gctgggtgcc accagatacc aacgttgaag agttcgaatt tgccattcgt acggtctgtg
    18241 aacctatctt tgagaaaccg ctggccgaaa tttcgtttgg acatgtactg ttaaatctgt
    18301 ttaatacggc gcgtcgcttc aatatggaag tgcagccgca actggtgtta ctccagaaaa
    18361 ccctgctcta tgtcgaaggg gtaggacgcc agctttatcc gcagctcgat ttatggaaaa
    18421 cagcgaagcc tttcctggag tcgtggatta aagatcaggt cggtattcct gctctggtga
    18481 gagcatttaa agaaaaagcg ccgttctggg tcgaaaaaat gccagaactg cctgaactgg
    18541 tttacgacag tttgcgccag ggcaagtatt tacagcacag tgttgataag attgcccgcg
    18601 agcttcagtc aaatcatgta cgtcagggac aatcgcgtta ttttctcgga attggcgcta
    18661 cgttagtatt aagtggcaca ttcttgttgg tcagccgacc tgagtggggg ctgatgcccg
    18721 gctggttaat ggcaggtggt ctgatcgcct ggtttgtcgg ttggcgcaaa acacgctgat
    18781 tttttcatcg ctcaaggcgg gccgtgtaac gtataatgcg gctttgttta atcatcatct
    18841 accacagagg aacatgtatg ggtggtatca gtatttggca gttattgatt attgccgtca
    18901 tcgttgtact gctttttggc accaaaaagc tcggctccat cggttccgat cttggtgcgt
    18961 cgatcaaagg ctttaaaaaa gcaatgagcg atgatgaacc aaagcaggat aaaaccagtc
    19021 aggatgctga ttttactgcg aaaactatcg ccgataagca ggcggatacg aatcaggaac
    19081 aggctaaaac agaagacgcg aagcgccacg ataaagagca ggtgtaatcc gtgtttgata
    19141 tcggttttag cgaactgcta ttggtgttca tcatcggcct cgtcgttctg gggccgcaac
    19201 gactgcctgt ggcggtaaaa acggtagcgg gctggattcg cgcgttgcgt tcactggcga
    19261 caacggtgca gaacgaactg acccaggagt taaaactcca ggagtttcag gacagtctga
    19321 aaaaggttga aaaggcgagc ctcactaacc tgacgcccga actgaaagcg tcgatggatg
    19381 aactacgcca ggccgcggag tcgatgaagc gttcctacgt tgcaaacgat cctgaaaagg
    19441 cgagcgatga agcgcacacc atccataacc cggtggtgaa agataatgaa gctgcgcatg
    19501 agggcgtaac gcctgccgct gcacaaacgc aggccagttc gccggaacag aagccagaaa
    19561 ccacgccaga gccggtggta aaacctgctg cggacgctga accgaaaacc gctgcacctt
    19621 ccccttcgtc gagtgataaa ccgtaaacat gtctgtagaa gatactcaac cgcttatcac
    19681 gcatctgatt gagctgcgta agcgtctgct gaactgcatt atcgcggtga tcgtgatatt
    19741 cctgtgtctg gtctatttcg ccaatgacat ctatcacttg gtatccgcgc cattgatcaa
    19801 gcagttgccg caaggttcaa cgatgatcgc caccgacgtg gcctcgccgt tctttacgcc
    19861 gatcaagctg acctttatgg tgtcgctgat tctgtcagcg ccggtgattc tctatcaggt
    19921 gtgggcattt atcgccccag cgctgtataa gcatgaacgt cgcctggtgg tgccgctgct
    19981 ggtttccagc tctctgctgt tttatatcgg catggcattc gcctactttg tggtctttcc
    20041 gctggcattt ggcttccttg ccaataccgc gccggaaggg gtgcaggtat ccaccgacat
    20101 cgccagctat ttaagcttcg ttatggcgct gtttatggcg tttggtgtct cctttgaagt
    20161 gccggtggca attgtgctgc tgtgctggat ggggattacc tcgtcagaag acttacgcaa
    20221 aaaacgcccg tatgtgctgg ttggtgcatt cgttgtcggg atgttgctga cgccgccgga
    20281 tgtcttctcg caaacgctat tggcgatccc gatgtactgt ctgtttgaaa tcggtgtctt
    20341 cttctcacgc ttttacgttg gtaaagggcg aaatcgggaa gaggaaaacg acgctgaagc
    20401 agaaagcgaa aaaactgaag aataaattca accgcccgtc agggcggttg tcatatggag
    20461 tacaggatgt ttgatatcgg cgttaatttg accagttcgc aatttgcgaa agaccgtgat
    20521 gatgttgtag cgcgcgcttt tgacgcggga gttaatgggc tactcatcac cggcactaac
    20581 ctgcgtgaaa gccagcaggc gcaaaagctg gcgcgtcagt attcgtcctg ttggtcaacg
    20641 gcgggcgtac atcctcacga cagcagccag tggcaagctg cgactgaaga agcgattatt
    20701 gagctggccg cgcagccaga agtggtggcg attggtgaat gtggtctcga ctttaaccgt
    20761 aacttttcga cgccggaaga gcaggaacgc gcttttgttg ctcagctacg cattgccgca
    20821 gaattaaaca tgccggtatt tatgcactgt cgcgatgccc acgagcggtt tatgacattg
    20881 ctggagccgt ggctggataa actgcctggt gcggttcttc attgctttac cggcacacgc
    20941 gaagagatgc aggcgtgcgt ggcgcgtgga atttatatcg gcattaccgg ttgggtttgc
    21001 gatgaacgac gcgggctgga gctgcgggaa ttgttgccgt tgattccggc ggagaaattg
    21061 ctgatcgaaa cagatgcgcc gtatctgctc ccgcgcgatc tcacgccaaa gccatcatcc
    21121 cggcgcaacg agccagccca tctgccccat attttgcaac gtattgcgca ctggcgtgga
    21181 gaagatgccg catggctggc tgccaccacg gatgccaatg tcaaaacact gtttgggatt
    21241 gcgttttaga gtttgcggaa ctcggtgttc ttcacactgt gcttaatctc tttattaata
    21301 agattaagca atagcatgga gcgagcctca ccatcgggtt cggtgaaaat ggcctgaaag
    21361 ccttcgaacg cgccttcggt aataatcacc ttatcacccg gataaggggg ggccggatcg
    21421 acaatgtctt tcggtttata taccgatagc tgatgaatta ccgccgatgg gactatcgct
    21481 ggcgacgcgc caaagcgcac aaagtggctg acgccacgcg tagcgctgat ggtcgtggta
    21541 tgaatcactt ctggatcaaa ttccacaaac aggtagttag ggaataacgg ttcactgact
    21601 gcggtacgtt taccccgtac gattttttcc agggtgatca tcggagcgag gcaattaacc
    21661 gcctgtctct caaggtgttc ctgggcacgt tgaagttgcc cgcgcttgca gtacagtaaa
    21721 taccaggatt gcataatgac tcttatccgt ttaatcgggg cgcaaggata gcaaaagctt
    21781 tacgctaagt taattatatt ccccggtttg cgttataccg tcagagttca cgctaattta
    21841 acaaatttac agcattgcaa agatgaacgc cgtataatgg gcgcagatta agaggctaca
    21901 atggacgcca tgaaatataa cgatttacgc gacttcctga cgctgcttga acagcagggt
    21961 gagctaaaac gtatcacgct cccggtggat ccgcatctgg aaatcactga aattgctgac
    22021 cgcactctgc gtgctggtgg gcctgcgctg ttgttcgaaa accctaaagg ctactcaatg
    22081 ccggtgctgt gcaacctgtt cggtacgcca aagcgcgtgg cgatgggcat ggggcaggaa
    22141 gatgtttcgg cgctgcgtga agttggtaaa ttattggcgt ttctgaaaga gccggagccg
    22201 ccaaaaggtt tccgcgacct gtttgataaa ctgccgcagt ttaagcaggt gttaaacatg
    22261 ccgacaaagc gactgcgtgg tgcgccctgc caacaaaaaa tcgtctctgg cgatgacgtc
    22321 gatctcaatc gcattcccat tatgacctgc tggccggaag atgccgcgcc gctgattacc
    22381 tgggggctga ccgtaacgcg cggcccgcat aaagagcggc agaatctggg catttatcgc
    22441 cagcagctga ttggtaaaaa caaactgatt atgcgctggc tgtcgcatcg cggcggcgcg
    22501 ctggattatc aggagtggtg tgcggcgcat ccgggcgaac gtttcccggt ttctgtggcg
    22561 ctgggtgccg atcctgccac gattctcggt gcagtcaccc ccgttccgga tacgctttca
    22621 gagtatgcgt ttgccggatt gctacgcggc accaaaaccg aagtagtaaa gtgtatttcc
    22681 aatgacctcg aagtgccagc cagtgccgaa atcgtcctgg aagggtatat cgatcctggt
    22741 gagatggcgc cggaagggcc gtatggtgac cacacaggtt actataatga agtcgataat
    22801 ttcccggtgt ttaccgtgac gcatattacc cagcgtgaag atgcgattta ccattccacc
    22861 tataccgggc gtccgccaga tgagcccgcg gtactgggcg tggcgttgaa cgaagtgttt
    22921 gtaccgattc tgcaaaaaca gttcccggaa attgtcgatt tttacctgcc gccggaaggc
    22981 tgttcttatc gtctggcggt agtgacgatc aaaaaacagt acgccggaca cgcgaagcgc
    23041 gtcatgatgg gcgtctggtc gttcttacgc cagtttatgt acactaaatt tgtgatcgtt
    23101 tgcgatgatg acgtcaacgc acgcgactgg aacgatgtga tttgggcgat taccacccgt
    23161 atggacccgg cgcgggatac tgttctggta gaaaatacgc ctattgatta tctggatttt
    23221 gcctcgcctg tctctgggct gggttcaaaa atggggctgg atgccacgaa taaatggccg
    23281 ggggaaaccc agcgtgaatg gggacgtccc atcaaaaaag atccagatgt tgtcgcgcat
    23341 attgacgcca tctgggatga actggctatt tttaacaacg gtaaaagcgc ctgatgcgcg
    23401 tttgttttgc cctatttatc gatccgacag agaaagcgca tgacaacctt aagctgtaaa
    23461 gtgacctcgg tagcagctat cacggatacc gtatatcgtg tccgcatcgt gccagacgcg
    23521 gccttttctt ttcgtgctgg tcagtatttg atggtagtga tggatgagcg cgacaaacgt
    23581 ccgttctcaa tggcctcgac gccggatgaa aaagggttta tcgagctgca tattggcgct
    23641 tctgaaatca acctttacgc gaaagcagtc atggaccgca tcctcaaaga tcatcaaatc
    23701 gtggtcgacc ttccccacgg agaagcgtgg ctgcgcgatg atgaagagcg tccgatgatt
    23761 ttgattgcgg gcggcaccgg gttctcttat gcccgctcga ttttgctgac agcgttggcg
    23821 cgtaacccaa accgtgatat caccatttac tggggcgggc gtgaagagca gcatctgtat
    23881 gatctctgcg agcttgaggc gctttcgtta aagcaccttg gtctgcaagt ggtgccggtg
    23941 gttgaacaac cggaagcagg ctggcgtggg cgtactggta ccgtgttaac ggcggtattg
    24001 caggatcatg gtacgctggc agagcatgat atctatattg ccggacgttt tgagatggcg
    24061 aaaattgccc gtgacctgtt ttgcagtgag cgtaatgcgc gggaagatcg cctgtttggc
    24121 gatgcgtttg catttatctg agatataaaa aaacccgccc ctgacaggcg ggaagaacgg
    24181 caactaaaac tttattcagt ggcatttaga tctatgtcgc atccggcaat tggtgcacga
    24241 tgcctgatgc gacgctggcg cgtcttatca ggcctacatt ggtgccggat cggtagaccg
    24301 gataaggcgt tcacgccgca tccggcaagt ggttaaaccc gctcaaacac cgtcgcaata
    24361 ccctgaccca gaccgataca catcgtcgcc agaccaaact gaacgtcttt gcgttccatc
    24421 agattcagca gcgtggtgct gatacgcgca ccggaacaac ccagcggatg acccagcgcg
    24481 atcgcaccac cattgaggtt gatcttctcg tcaatctgct ccattagccc gagatcttta
    24541 atgcacggca ggatctgcgc ggcaaaggct tcgttcatct caaacacgcc gatatcgctg
    24601 gcagaaagcc ccgctttttt cagcgccagc ttcgaggccg gaaccgggcc gtaacccata
    24661 atcgatgggt cacaaccaac gaccgccatc gaacgcacac gagcgcgcgg cttaagacct
    24721 aattcatggg cgcggctttc actcatcacc agcatggcag ctgcgccatc ggaaagtgca
    24781 gaagatgtgc ccgccgttac cgtaccgttt actggatcaa acgccggacg cagcgtggcg
    24841 agggcttcca cggtggtttc cgggcgaatc acttcgtcgt aattaaactg cttcaggacg
    24901 ccgtcggcat cgtgaccacc agttgggatg atttcatttt taaatgcggc cgactgcgtg
    24961 gcggcccagg cgcgggcgtg tgaccgcgcg gcaaaggcat cctgcatttc acggctgata
    25021 ccgtgcatac gcgccagcat ttctgccgtt aagcccatca tgcccgccgc tttggcgaca
    25081 ttgcggctca ggccggggtg aaaatcgacg ccgtgactca tcggcacatg gcctatatgc
    25141 tccacgccgc caaccagaca tgcctgcgta tcgccagtca tgatcattcg tgctgcgtca
    25201 tgcagtgcct gcatggatga accacacaag cgattgacgg taaccgccgg gacagagtgc
    25261 ggcacttctg ccagcagcgc cgcgttacgg gcgatgttaa aaccctgctc cagcgtctgt
    25321 tgcacacaac cccagtaaat atcgtcgagg gccgccgctt ccagcgccgg gttacgcgcc
    25381 agcaggctac gcattaaatg agcggagaga tcttccgcac gcacgttacg aaaagcaccg
    25441 cccttcgaac ggcccatcgg ggtgcgaatt gcatcgacaa tgacaacctg ttccattgtg
    25501 actccttaag ccgttttcag gtcgccaacc ggacgggctg gctcaaccgg aggatagtac
    25561 ggttcgttat gacgcgcttt attacgcaga ccttccggca cttcatacag cgggccgagg
    25621 tgctgatatt gctgtgccat atcgaggtat tttgcgctac cgagggtgtc cagccagcgg
    25681 aacgcgccgc cgtggaacgg agggaagccc aggccgtaga ccagcgccat atccgcttcc
    25741 gccggagtgg cgataatgcc ctcttccaga cagcgcacca cttcgttgac catcgggatc
    25801 atcatgcggg cgataatctc ttcttcgctg aaatcgcgct tcggttggct cacttctgcc
    25861 agcaggtctt caacggcggc gtcttcttct ttcttcggct tacctttgct gtcttcttta
    25921 taacgccaga aaccgaggcc gttcttctga ccaaagcggt tggcatcaaa cagcgcgtcg
    25981 atggcatcgc ggtaatcttt ctgcatccgc tgcgggaagc ctgctgccat gacagcctga
    26041 gcgtgatgcg cggtatcaat gcccacaacg tccagcagat atgccgggcc catcggccag
    26101 ccaaactgtt tttccatcac tttgtcgatc ttgcggaaat ccgcgccgtc gcgcagcagc
    26161 tggctgaaac cggcgaaata cgggaacagc acgcggttaa caaagaagcc ggggcagtcg
    26221 ttaaccacaa tcggcgtctt gcccatcttg ctcgcccagg cgacaacttt cgcgatggtt
    26281 tcgtcggagc ttttctcgcc gcgaataatt tctaccaacg gcattcggtg gaccgggtta
    26341 aagaagtgca tcccgcagaa gttttccggg cgttccagcg cgttggccag ttcgctgata
    26401 ggaatggttg aagtgttaga cgccagcacg gtatcctggc gtactttttg ttcggtttct
    26461 gccagtacgg cttttttcac tttcgggttt tcaacaaccg cttctaccac aatatccacg
    26521 cggtcaaatc cggcgtagtc gagcgttggg tggatcgtgg agatcacgcc agccagtttc
    26581 agaccatcga ttttgccgcg ctcaagctgc ttgttcagca gtttcgcggc ttcggtcatg
    26641 ccgagggtta acgacttgtc gttgatatct ttcatgacaa ccggcacgcc tttccacgcg
    26701 gactggtaag cgataccgcc acccatgatg cctgcgccca gcacagcagc ctgtttcggg
    26761 gtttcaacgt ctttggtgag tttcttcgct ttgcctttta catattgatc gttaaggaaa
    26821 atgccgacca gtgcgcgagc ttcgttggta tgcgccagcg ggacaaaact tttgttttcc
    26881 agatttaagg cttcttcacg accaaaacgg gccgcagctt caatggtttt tactgcggtg
    26941 atgggggccg gataatgttt ccccgccgtt tgcgcgacca ttcctttagc gatggtgaag
    27001 ctcatggtgg cttcaatctt gctcagtttt agcggttcct gcttcggctg acgtttcgct
    27061 ttccagtcga ggtcgccgtt aatggcctgg cgtaaaatcg ccatcgcccc ttcaatcagt
    27121 ttttctgctt tgactacgcc atccaccaga ccgattttca gcgcctgatc cgcgccgaca
    27181 tctttaccgg cggcaatgat ttccagtgca ctgtcagcgc ccagcatacg tggcatacgt
    27241 acagaaccgc caaagcccgg catgatgccc agtttggttt ccggcagacc gatgcgcaga
    27301 tccggcgtcg ccagacgata atcggtcgcc agcacgcatt cgcagccacc acccagcgca
    27361 tagccgttga cggcagcaat ggtcggcacc ggcagatctt ccaggcgatt aaacacgcta
    27421 ttggcaaagt gcagccactg acttaactgt tcttcaggaa cgaggaacag ggacaaaaat
    27481 tcggtgatat cagcaccgac gataaaggct gctttgttcg aacgcagcag cagccctttt
    27541 agatctgatt gctgttccag cacgccgatg gcctcgccga ggctggcgac ggtcgcagtg
    27601 tcgagtttat taactgaacc tggggcatca aataccagtt cggcaatgcc atcttccagc
    27661 cagtcaaggt acagggtgtc gcctttgtaa agcatgtcag tctcctgaat ccgcaaggta
    27721 atctggtcgt accagatgca tcggagtgtg tgttttgtgt taaaaatatg caaataaaag
    27781 attaaagaaa tgccgatctg atcacgctcg gcagaaatca cgctctggat gaacgatgtg
    27841 ctaagatgcg gagacttaag gtcaaaaaaa cagaagggta aaaaatggaa tcactggcct
    27901 cgctctataa aaatcatata gctaccttac aagaacggac tcgcgatgcg ctggcgcgct
    27961 tcaagctgga tgcattactt attcactccg gcgagctgtt caacgttttt ctcgacgatc
    28021 atccctatcc gtttaaagtg aacccgcaat tcaaagcgtg ggtgccggta actcaggtgc
    28081 caaactgctg gttgctggtg gatggcgtga acaagccgaa actgtggttc tatctgccgg
    28141 ttgattactg gcacaacgtc gaaccgctgc cgacctcctt ctggactgaa gatgtggaag
    28201 tgatcgcgct gccgaaagcc gatggcattg gtagcctgct gcctgctgcg cgcggcaata
    28261 tcggttatat cggtccggtg ccggaacgtg cgctgcaact gggtattgag gccagcaaca
    28321 tcaacccgaa aggggtgatc gactacctgc attattaccg ctccttcaaa accgagtacg
    28381 aactggcctg tatgcgtgaa gcgcagaaaa tggcggtcaa tggtcatcgt gcggcagaag
    28441 aagcgttccg ttctggcatg agcgagttcg atatcaatat tgcctatctg accgcgaccg
    28501 gtcatcgtga taccgacgta ccttacagca acattgtggc gcttaacgaa cacgctgcgg
    28561 tgttgcatta caccaaactg gaccaccagg caccggaaga gatgcgcagc ttcctgctgg
    28621 atgccggggc cgaatataac ggctatgcgg ctgacctgac ccgtacctgg tcggcaaaaa
    28681 gcgacaacga ctacgcacag ctggtgaaag acgtaaatga tgaacaactg gcgctgatcg
    28741 ccaccatgaa agcaggcgtc agctatgtgg attaccacat ccagttccat cagcgcatcg
    28801 ccaaactgct gcgtaaacat caaatcatca ccgatatgag tgaagaagcg atggtcgaaa
    28861 acgatctcac cggaccgttt atgccgcacg gtattggcca tccgctgggc ctgcaggtgc
    28921 atgacgtcgc cggttttatg caggatgata gcggtacaca cctcgcggca ccggcaaaat
    28981 atccgtacct gcgctacacc cgtattctcc agccgggcat ggtgttaacc atcgaaccgg
    29041 gtatctactt cattgaatcg cttctggcac cgtggcgtga agggcagttc agcaagcact
    29101 tcaactggca gaaaattgaa gcactgaaac cgttcggcgg cattcgtatc gaagacaacg
    29161 tggtgatcca cgaaaacaac gtggaaaaca tgacccggga tctgaaactg gcgtgatgga
    29221 aagctggtta attcctgcgg caccggtcac ggtcgttgaa gagatcaaaa agagccgctt
    29281 cataacgatg ttggcgcata ccgatggcgt tgaggcggcg aaagcgtttg ttgaatcggt
    29341 gcgggcagaa caccccgatg cccgccacca ttgcgtggcg tgggtggcgg gtgcgccgga
    29401 tgattctcaa cagctgggtt tctctgacga cggggagccg gcgggaacgg caggtaaacc
    29461 gatgctcgcc cagctaatgg gcagcggcgt cggggaaatt accgctgtgg tagtgcgcta
    29521 ctacggcggc atattgcttg gcaccggtgg gttagtgaaa gcgtatggcg gcggcgtgaa
    29581 tcaggcgctg cgccagctaa cgacccaacg caagacgcca ttaaccgaat atactttgca
    29641 atgtgaatat agtcagttaa ccggcattga agcgttgctg gggcagtgtg acggcaaaat
    29701 tatcaacagt gattatcagg cattcgttct gctgcgggtg gcgcttccgg cggcgaaagt
    29761 ggctgaattt tccgcaaagc tggcggattt tagccgtggt tcattgcaat tgttagcgat
    29821 tgaagaataa tccccacttc gttttgcaga actaaggaag cggcagagat gcattttcgc
    29881 gccattaccc gaatcgttgg actactggtc atcttatttt cagggaccat gattatccct
    29941 gggctggtag cactcatcta ccgggatgga gcgggccgcg cttttaccca gacctttttt
    30001 gtcgccctcg ccattggctc tatgctgtgg tggccgaacc gcaaagagaa gggcgaactg
    30061 aaatcccgtg aggggtttct gatagtggtg ctgttttgga ccgtgctggg tagtgtcggt
    30121 gcgctccctt ttatcttctc ggaaagcccg aacctcacga ttaccgatgc gttttttgaa
    30181 tctttctctg gcctgaccac caccggggcc actacgctgg tggggctgga ttcgctccct
    30241 catgccatcc tcttttatcg ccagatgctg caatggtttg gcgggatggg gatcatcgtg
    30301 ttagcggttg cgatactgcc tatcctcggc gtgggtggga tgcagctcta tcgcgcagaa
    30361 atgcccggcc cgctgaaaga taacaaaatg cgcccgcgaa ttgcggaaac ggcgaaaact
    30421 ctgtggttga tttatgtctt gctgaccgtc gcctgtgcgc tggcgttgtg gtttgccgga
    30481 atggatgcct ttgatgccat cggccatagc tttgcgacta tcgctattgg cggcttctcg
    30541 acacatgatg ccagtatcgg ttatttcgac agtccgacta ttaacactat cattgctatc
    30601 ttcctgctga tctccggctg taactacggt ctgcactttt cactgttaag tgggcgtagt
    30661 ctgaaggttt attggcgcga tccggaattt cgcatgttta tcggcgtaca gtttacgctg
    30721 gtggttattt gtacactcgt actgtggttt cataatgtct acagttcggc gctgatgaca
    30781 attaaccagg cgtttttcca ggtggtatcg atggcgacaa ccgccgggtt tacgactgac
    30841 agcattgccc gctggccgct ctttttgccg gtactgcttt tatgttcagc ttttatcggc
    30901 ggttgcgccg ggtcaacggg cggtggcctg aaagtgatcc gcatcctgct gctgtttaag
    30961 caggggaacc gtgagctgaa acgactggtg catccgaacg ccgtctatag cattaagctg
    31021 gggaatcgcg cactgccgga acgtatcctc gaagccgttt ggggattttt ctccgcctat
    31081 gcattggtgt ttattgtcag tatgctggcg attatcgcca cgggcgtgga tgatttttct
    31141 gcctttgcct cagttgttgc gacattgaat aacctggggc cagggcttgg cgtggttgct
    31201 gataacttta ccagtatgaa cccggtggct aaatggatcc tgattgccaa catgctgttt
    31261 ggtcgtctcg aggtctttac attactggtg ctctttaccc cgactttctg gcgtgaatga
    31321 tggagtaata cgtgaaaaca ttaattcttt tctcaacaag ggacggacaa acgcgcgaga
    31381 ttgcctccta cctggcttcg gaactgaaag aactggggat ccagacggat gtcgccaatg
    31441 tgcaccgcat tgaagaacca caatgggaaa actatgaccg tgtggtcatt ggtgcttcta
    31501 ttcgctatgg tcactaccac tctgcgttcc aggaatttgt caaaaaacat gcgacgcgac
    31561 tgaattcgat gccgagcgcc ttttattccg taaacctggt ggcgcgcaaa ccggagaagc
    31621 gtactccaca gaccaacagc tacgcgcgga agtttctgat gaactcgcaa tggcgtcccg
    31681 atcgctgcgc ggtcattgcc ggggcgctgc gttacccacg ttatcgctgg tacgaccgtt
    31741 ttatgctcaa gctgattatg aagatgtcag gcggtgaaac ggatacgcgc aaagaagttg
    31801 tctataccga ttgggagcag gtggcgaatt tcgcccgaga aatcgcccat ttaaccgaca
    31861 aaccgacgct gaaataagca taaagaataa aaaatgcgcg gtcagaaaat tattttaaat
    31921 ttcctcttgt caggccggaa taactcccta taatgcgcca ccactgacac ggaacaacgg
    31981 caaacacgca gccgggtcag cggggttctc ctgagaattc cgacagagaa agcaaaaata
    32041 aatgcttgac tttgtagcgg gaaagcgtat tatgaacacc ccgcgc
//
LOCUS       DAZSIO010000028        31785 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZSIO010000028 DAZSIO010000000
VERSION     DAZSIO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.028"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(245..1249)
                     /gene="trpS"
                     /locus_tag="ACHX5T_001204"
     CDS             complement(245..1249)
                     /gene="trpS"
                     /locus_tag="ACHX5T_001204"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR8466477.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(1242..2000)
                     /gene="gph"
                     /locus_tag="ACHX5T_001205"
     CDS             complement(1242..2000)
                     /gene="gph"
                     /locus_tag="ACHX5T_001205"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR8466478.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(1993..2670)
                     /gene="rpe"
                     /locus_tag="ACHX5T_001206"
     CDS             complement(1993..2670)
                     /gene="rpe"
                     /locus_tag="ACHX5T_001206"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR8466479.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(2688..3524)
                     /gene="dam"
                     /locus_tag="ACHX5T_001207"
     CDS             complement(2688..3524)
                     /gene="dam"
                     /locus_tag="ACHX5T_001207"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR8466480.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(3631..4923)
                     /gene="damX"
                     /locus_tag="ACHX5T_001208"
     CDS             complement(3631..4923)
                     /gene="damX"
                     /locus_tag="ACHX5T_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR8466481.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(5015..6103)
                     /gene="aroB"
                     /locus_tag="ACHX5T_001209"
     CDS             complement(5015..6103)
                     /gene="aroB"
                     /locus_tag="ACHX5T_001209"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR8466482.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(6160..6681)
                     /gene="aroK"
                     /locus_tag="ACHX5T_001210"
     CDS             complement(6160..6681)
                     /gene="aroK"
                     /locus_tag="ACHX5T_001210"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR8466483.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(7082..8320)
                     /gene="hofQ"
                     /locus_tag="ACHX5T_001211"
     CDS             complement(7082..8320)
                     /gene="hofQ"
                     /locus_tag="ACHX5T_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR8466484.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVMPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(8232..8638)
                     /gene="hofP"
                     /locus_tag="ACHX5T_001212"
                     /pseudo
     CDS             complement(8232..8638)
                     /gene="hofP"
                     /locus_tag="ACHX5T_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(8628..9068)
                     /gene="hofO"
                     /locus_tag="ACHX5T_001213"
     CDS             complement(8628..9068)
                     /gene="hofO"
                     /locus_tag="ACHX5T_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR8466485.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(9052..9591)
                     /gene="hofN"
                     /locus_tag="ACHX5T_001214"
     CDS             complement(9052..9591)
                     /gene="hofN"
                     /locus_tag="ACHX5T_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFR8466486.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(9591..10370)
                     /gene="hofM"
                     /locus_tag="ACHX5T_001215"
     CDS             complement(9591..10370)
                     /gene="hofM"
                     /locus_tag="ACHX5T_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR8466487.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            10490..13042
                     /gene="mrcA"
                     /locus_tag="ACHX5T_001216"
     CDS             10490..13042
                     /gene="mrcA"
                     /locus_tag="ACHX5T_001216"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR8466488.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(13207..13767)
                     /gene="nudE"
                     /locus_tag="ACHX5T_001217"
     CDS             complement(13207..13767)
                     /gene="nudE"
                     /locus_tag="ACHX5T_001217"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR8466489.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            14087..16222
                     /gene="igaA"
                     /locus_tag="ACHX5T_001218"
     CDS             14087..16222
                     /gene="igaA"
                     /locus_tag="ACHX5T_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR8466490.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            16287..16955
                     /gene="yrfG"
                     /locus_tag="ACHX5T_001219"
     CDS             16287..16955
                     /gene="yrfG"
                     /locus_tag="ACHX5T_001219"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR8466491.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            16966..17367
                     /gene="hslR"
                     /locus_tag="ACHX5T_001220"
     CDS             16966..17367
                     /gene="hslR"
                     /locus_tag="ACHX5T_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR8466492.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            17392..18261
                     /gene="hslO"
                     /locus_tag="ACHX5T_001221"
     CDS             17392..18261
                     /gene="hslO"
                     /locus_tag="ACHX5T_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR8466493.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(18405..20163)
                     /locus_tag="ACHX5T_001222"
                     /pseudo
     CDS             complement(18405..20163)
                     /locus_tag="ACHX5T_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            20542..22164
                     /gene="pckA"
                     /locus_tag="ACHX5T_001223"
     CDS             20542..22164
                     /gene="pckA"
                     /locus_tag="ACHX5T_001223"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR8466494.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(22240..23592)
                     /gene="envZ"
                     /locus_tag="ACHX5T_001224"
     CDS             complement(22240..23592)
                     /gene="envZ"
                     /locus_tag="ACHX5T_001224"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR8466495.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(23589..24308)
                     /gene="ompR"
                     /locus_tag="ACHX5T_001225"
     CDS             complement(23589..24308)
                     /gene="ompR"
                     /locus_tag="ACHX5T_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR8466496.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            24536..25012
                     /gene="greB"
                     /locus_tag="ACHX5T_001226"
     CDS             24536..25012
                     /gene="greB"
                     /locus_tag="ACHX5T_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR8466497.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            25109..27430
                     /locus_tag="ACHX5T_001227"
     CDS             25109..27430
                     /locus_tag="ACHX5T_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR8466498.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            27868..28095
                     /gene="feoA"
                     /locus_tag="ACHX5T_001228"
     CDS             27868..28095
                     /gene="feoA"
                     /locus_tag="ACHX5T_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR8466499.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            28112..30433
                     /gene="feoB"
                     /locus_tag="ACHX5T_001229"
     CDS             28112..30433
                     /gene="feoB"
                     /locus_tag="ACHX5T_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR8466500.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            30433..30669
                     /gene="feoC"
                     /locus_tag="ACHX5T_001230"
     CDS             30433..30669
                     /gene="feoC"
                     /locus_tag="ACHX5T_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR8466501.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            30872..31759
                     /gene="rpnA"
                     /locus_tag="ACHX5T_001231"
     CDS             30872..31759
                     /gene="rpnA"
                     /locus_tag="ACHX5T_001231"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFR8466502.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
BASE COUNT         7650 a         8600 c         8111 g         7424 t
ORIGIN      
        1 ttatatcttc attaaaatga agataagtgg cgacgacgag atgtggaaac ggcgaggcgc
       61 ttcacatttt ttcgggttgt tgcgaaaaga ggataaaaaa accgggtttc cccggtttca
      121 gagtgatgac gaagtgattt attcgacaat gcgagcaaag ggtaattccc actggcgctt
      181 gcctgctccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtc cgagcgtttt
      241 gatattacgg cttcgccaca aaaccaatcg cttcgtacac cgctttcagc gtacgggaag
      301 cgtgtacact ggctttttct gcgccgtctt tcatcacctg ttgcaggaag gcttcatcgt
      361 tgcggaaacg gtgatagcgt tcctgcaatt cagtcagcat accggaaacg gcatcagcca
      421 cttcaccttt cagatgacca tacatcttgc cttcgaactg tttttccagt tctgggatgc
      481 tctggcccgt taccgcagaa aggatatcca gcaggttgga aacgcccgct ttgttctgca
      541 catcgtagcg aactaccggc ggctcgtcgg agtcagtgac cgcgcgtttg attttcttca
      601 ctaccgattt cggatcttcc agcaggccga taacgttatt gcggttatcg tcagacttgg
      661 acatcttctt ggtcggctcc agcagcgaca ttacgcgcgc gccagatttc ggaataaacg
      721 gctccggcac cttaaagatc tcgccataca gcgcgttgaa acgctgggcg atatcgcgac
      781 tcagttccag gtgctgtttc tggtcttcac ccaccggtac cagattagtt tgatacagca
      841 ggatgtccgc agccatcagc accggatagc caaacagacc agcgttgatg ttctcggcat
      901 aacgcgcaga tttatcttta aactgcgtca tgcgactcag ttcgccgaag taggtatagc
      961 agttcagtgc ccagcctaac tgcgcatgtt ctggtacgtg ggactgaaca aaaatggtgc
     1021 ttttctcagg atcgatacca caagccagat acaaggccag cgtatccaac gtcgctttac
     1081 gcagcttctg tgcatcctgg cgcacggtga tcgcgtgttg gtcaacgata cagtaaatgc
     1141 aatggtagtc atcctgcatg tttacccact gacgcagcgc acccatgtag ttaccaatgg
     1201 tcaattcacc tgagggctgt gcgccactaa aaacgatggg cttagtcatt tttcgattcc
     1261 tgattttcgc tatgcggaag ccctaatgcg ggcagaaggt catttataga ctgataaatt
     1321 acatcaggct ggctgagatc gatagcctcg ccgtagttgt atccgtaggt taagccaact
     1381 gatgggcaac ctgccgcttt tgccgcctga atatcattcc gtgagtcgcc gacaaacagc
     1441 atctgttgtg gggcaattcc catccgctca gccaccagta acagcgggtc cggatgcggt
     1501 tttttgtttt gcacatcatc gccgccgata accacgctaa agtatttggc aatatctaag
     1561 gcttcgagca gcggcgcgac gaacggcgtc ggtttgttgg tgaccaagcc tagcggcagg
     1621 cctttagcct gcaacgcgcc caacgtatcg gcaacgtgcg ggaacaaaaa cgtcccctct
     1681 tcggcaacct cgccatagta gcgatcgaac agtttacgca gaatacgtac ctgttcttct
     1741 gccggaatgt cgtcatcaac gggcggtttc cccattgttt tacgcagagt cgcacgttcc
     1801 tgacgcgccc aggtcaatgc gcgctccatc agaacatctg cgccgttacc aatccaggta
     1861 ataacgcgtt cttcacctgc gacgggcaac tccagcgcat acagcgccat atctaccgca
     1921 gcagcaagac caggagcact gtcgaccagc gtaccatcaa gatcaaaagc gacgccgcga
     1981 atatcttcaa acttattcat gacttacctt tgccagttca ctgcgcattt catcaatgac
     2041 ttttttatag tctggctggt cgaagattgc cgaaccggcg acgaacatat ccgcgcccgc
     2101 cgcagcgatt tcgccaatgt tgttcacctt cacgccaccg tccacttcca gacgaatgtc
     2161 aaagccagac tcgtcgatac ggcgacgtac ttcgcgcagt ttatccagtg tttgaggaat
     2221 gaaagactga ccgccgaaac cagggttgac ggacatcagc aggatcacat ccagcttatc
     2281 catcacgtaa tccagatagc tcagaggtgt cgccgggtta aataccagac ccgctttaca
     2341 gccattttct ttaatcagtt gcagcgtgcg gtcaacatgc tcggaggctt ctggatgaaa
     2401 ggtaatgatg ctggcaccag cggcagcgaa atcaggcaca atgcgatcga cgggtttcac
     2461 catcagatgt acgtcgatag gggcggtaat gccgtagtta cgcaaggatt tcagcaccat
     2521 tggcccaatc gtcagattgg gaacatagtg gttatccatg acgtcaaaat gcacgacatc
     2581 agcgccagct gccagggctt ttgcggtatc ttcacccagg cgggcaaaat cagccgacag
     2641 aattgagggg gcaatcaaat actgtttcat ccgcttctcc ttgagaatta ttttttcgcg
     2701 ggtgaaacga ctcctggttt gtacaaagcc agcagttcgt ccaccttttt acgtgtgccg
     2761 ccgttgctgc ttatactgcg tcgaactttg acgacatgca attttgcgcg ctgataccac
     2821 tcacgcgtta acatcgtatc gtgattggag atcagcactg gaatatggcg ctcaaccaga
     2881 ccttcggcga tctccgccag atgcgcttgt tgttcaagcg taaaactgtt tgtgtgatac
     2941 gccgtaaagt tggcggtcgc agacagcggt gcataaggcg gatcgcaata gacgacggat
     3001 gcatcatctg cgcgctccat gctatcggcg taagactcac aatagaaaaa ggcattctgc
     3061 gctttttcag cgaagtgata caactctgct tccgggaaat agggtttttt gtagcggcca
     3121 aacggcacgt taaactcacc gcgcagattg taacgacaca ggccgttgta accgtagcgg
     3181 ttcaaatata aaaacagtac cgcccgacgg aacggatcct ggcttttgtt gaactcttcg
     3241 cggaactgat agtaaacctc ggcgcaattt gtttcgggaa caaacagctc gcgtgcggcc
     3301 tgtacgtact catcagtacg catcttcaca atgttataga gactgatcag gtcgctattg
     3361 atatcggcaa ggatataacg agaaaagtcg gtgttgagaa acaccgaccc ggcacctaca
     3421 aaaggctcaa ccagacattc gcccttgggc aaatgccgtt taatatcatc aagcagggga
     3481 tacttgcccc ctgcccactt caaaaaagcg cgatttttct tcatgccgat taactaatta
     3541 caccttctcc ggctgtggag aaagctccga cagcatccag cgcttcaggc agctcccgca
     3601 acgataaacc attgcgggag ataaccttga ttacttcaga tcggcctgta cctgacgcag
     3661 cggtttcgcc cacgggtttt tggcctgaac atctgctggc aatgtagata ccgctttttt
     3721 cgcctcttct ttcgaagcgt acacgccaga aaccaggaca taccacggct gaccattacg
     3781 cgtcgtttca tagacaacgt agtttttcag attctctttc ttcgcccaac cgttcaggtt
     3841 gtcgtagtta gaggaactgc tcagctgcag agtgtaatgg ctggacggtg ccgatttcaa
     3901 cgaaccaaca ttacctgcgg tcttccctcc agcggctggt gttgccgtgg tttgcgccgg
     3961 ggatgccgtc tgtactggag ccgtagtcgc cgtctctttt ggtgctggcg tagaagtcgc
     4021 agccggtgcc ttcgtgctcg ccacaggggc agctggttca ggttcagtac gcttcggcgt
     4081 ctgtgctacc ggcttcggct ccgttttcac ggttgcttgc ggttttttcg gttcaatcac
     4141 cgcctgctga cgaactgggc gcgtagtgga cggacgttca gcggtttgcg ttttcgccgt
     4201 gtcacgcgat gcattgccat tgcgaacagg cgcaaccgtt gcgggttcag tcggcaacgt
     4261 ggaattgacc gccacattgt tcagctgttg ctgattttgt ggctgggtca gcgcattgtt
     4321 caggtcaccc tgcacttcaa cacgttgttg accatccgtt gccaccgggg tttgcccttg
     4381 agtcggcgta gaagagatcg gtggcagaga aacatcctgc tgagtatttt ccgcagacgt
     4441 ggttcccggc gctggctgca cgccattcgc ctgatcggtc gcattgccag caagatcaat
     4501 actcttctcg ccagacgcgg tttgatcgct ggaagtggtc gagggggctt ttagcgcaga
     4561 accgataccg atgatcaaca gcagtagaac cagaatgccg acgcccatca tcatatactg
     4621 acgagaagcg ggtttactgg ctgctttttt gcgcttacgc ggacgacgct ctacgcgctc
     4681 ttcatccacg gtttcatctt cggattcgtc aatttcttct tcgatttccg gttcctcatt
     4741 gcgctctttt tgcgcacgag tcggacggcg atcgtcagtg tcatcaagtt caatatcatc
     4801 aaaattgatc tgcggttcgc cacgttcagt acgctcagaa cgttcagaag attgacgaga
     4861 acgaccagta cgacgatcgc tgggatcggg tttcagctcg tcttctggtt tgaattcatc
     4921 catttaacac cccactaaaa ggttaatgct taccacgttg caattaacct gaagctaata
     4981 gaccgcttga taagcggcct gacctttctt gttgttacgc tgattgacaa tcggcaatgg
     5041 cgttaagaac aagctcgtgc gaaacgccgc tgcgaacttc actcttacca attgccaacg
     5101 gaagaattaa gcgcatctct cccgcaagga ctttcttgtc acgcagcata tgcggtaaat
     5161 acgcctgcgc ggacatttcg cgcggcccat tgaccggtaa cccagcccgc gtgagcaggg
     5221 ttataatacg ctgcgtttcg gcagaactaa actgcccgag acgttccgac gtccgcgccg
     5281 ccatcaccat acccgcagcg accgcttcac catgtaacca attgccatac cccatttcag
     5341 cttcaatggc atgaccaaag gtgtgtccca gattcagtaa agcacgtaac ccggtttcgc
     5401 gctcgtcggc ggcgacaact tctgccttca gttcacaaca acggcgaata cagtacgcca
     5461 ttgccggacc gtccagacgc aacaacgcat ccagattctc ttccagccag ttaaaaaacg
     5521 caccgtcaag aataatgccg tatttgatga cttctgccag ccccgacgct aactcacgcg
     5581 ggggaagcgt tttcagacag tcgagatcca ccaccactga agcaggctgg tagaacgcgc
     5641 caatcatgtt tttaccgagg ggatggttga ccgcagtttt gccgccaacg gaggaatcga
     5701 cctgcgacag taacgtcgtc gggacttgaa tgaaacgaac accgcgctga taactcgccg
     5761 ccgcgaagcc ggtcagatcg cccactacgc cgccgccaag cgccaccagc gtagtatcgc
     5821 gaccatgcgg cttttgtaac aacgccgtaa agacggtatc gagtacagct aggcttttat
     5881 actgctcgcc gtcagggagg ataacgctat cgacgttaac acccgcctgt tcaagtacgc
     5941 cgcggacctt atcgagatac agaggagcca gggtttcgct ggtgaccaac atgacctgct
     6001 cgcccgattt cagcggtaag aatgaagctg gttcattaaa caaaccagat gcgatggtaa
     6061 ttgggtaact acgttccccg agagtaacga caatcctctc cataacgcga catccacctt
     6121 aattactgta cccgcagacg agtgtatata aagccagaat tagttgcttt ccagcatgtg
     6181 aataatctgg tttgcaacca ctttagcgct ttgatcatca gtacgaatgg tcacgtcggc
     6241 aatctcttca tacagcggat tgcgttcatt ggccaacgct tccagaactt cacgcggcgg
     6301 tgtttcaacg tgcagcaacg ggcgtttttt atcacgctgc gtgcgtgcaa gttgcttttc
     6361 gatggtcgtt tcaagataaa cgacaacgcc acgagcggaa agacggttac gcgtttcacg
     6421 ggatttcaca gagccgccgc cagtagccag cacaataccc tgtttctcgg tcaactcatt
     6481 gatgaccttt tcttcgcgat cgcggaagcc ttcttcgcct tctaaatcga aaacccagcc
     6541 cacatcagct ccggttcgtt tctcaatctc ttgatcggaa tcgtaaaatt ccatattgag
     6601 ttgttgagct aactggcgcc caatagtgct ttttccggca cccataggcc caaccagaaa
     6661 gatattgcgt ttctctgcca ttttttcggt actactaaga ctattcgtta atgataaacc
     6721 cgcttcgctc agagagcgcc gcaggacatg aactgaaacc tcataagata ttgcgagagt
     6781 cagactgaaa attatctcaa tactccagcg ggtttggcaa ctgaataaat cacccatccc
     6841 tctgcatatc tggtcgctgc aagcgcgctg ccttgctacc accgctctgg cgataaatca
     6901 ccgggtaaga ttagcgtaaa aaagacagca aaatgccgcc tgaatgataa atcatcatca
     6961 tggaacgcag gcagcgaaga gcaccaactc tcaaatcggt actccttgta tgctaaatac
     7021 ctgcgcgcgt caaatagatg aaacacgttc agcataaaaa cattaccgct tttacggctg
     7081 tttactcact ggaaaccagt cgtggcgtga taaacaccac taactcgcgt cgttcatctt
     7141 cttttccgtc atgacgaaat aattgcccga accagggaat gtcgccaagc aacggtacgc
     7201 tatcctgacc cgatttattt ttacgggtaa aaatgccgcc cagcgccaac gtttctccgc
     7261 tttttacctc gacctgcgtt tcgatctcct gcttatcaat cgccagcact tcgccatcgg
     7321 cctgctgtag cacctgcccc ggaacgttct ggctgatgtg taatttcagc cggatgcgac
     7381 ctttttgtaa caccgtgggc atgacctcca tccccaggac ggcctcttta aattccaccg
     7441 acgtcgcgcc actttccccg ctggaaacct gatatggaat ttcgctctcc tgtttaatgc
     7501 tggcaggctg aagatgtgag gccagcagac gcggactggc gataatatcc agctgctgtt
     7561 tttgttcgag cgcggaaagc tcaagatcca gcaagcgtcc gttgatgcgc ccaatgttaa
     7621 aaccgacatg cgttgtcgcc gtcgctacgg agaggtcgct accaagcgtg gtgacttgcc
     7681 caacgccacc agcgtgttgc gcatcggcca gcgtccattt cacgcctaac tcacgcaaac
     7741 ttttttcatt aatggtgaca atatgcgccg acagctcaac ctgcccgacc ggcagatcca
     7801 tttgcgctac ccactgttca agcgcgctta acgccgtttt gttatctcgt agcaaaaggc
     7861 gattggtgcg tttatcgacg gtcatactcc ctttggcact cagtagcttc tcccccgctt
     7921 tcgccagttc tcccgcgtcg gcgtattgca gggttatact gcgattttcc agcggcagat
     7981 ttgcctgcgc ccgcgcctgc tccgcctcct ggcgggcgat attgtcattc tgccaggcaa
     8041 tggaatgcac tgagagaatg ttgccttcct gccgcgttat cagtccggcg cttttcacta
     8101 cagtttgtag tgcctgcttc cagggaacat ctgttagatg taacgacacc gtaccgctga
     8161 cgtctggcga cacgaccagg ttcaacttct cctgttcagc cagcgcctgc aacacctgag
     8221 ctaccggaac gtcatccacc atcagcgtca ctttttgcgg ctttgccgcc tgtacgccgg
     8281 gtatcagcat caacagtagt gcggctatcc attgcttcat ttgtatctcc ttgccgttgc
     8341 cacaaccatt gtggcggttc gcagtttgtc ccggtaccca gcgttagtac gtctggcgtc
     8401 agctgtaaaa ttgtccagcc gttttccagc acatcgtttt gctgcacccg tcgccatttc
     8461 ttttgcccgt cttttattac accgatgatg cgctcgcctc gccctaccat cccctgatag
     8521 cgccactggc taagttcgct aatccggcat atagatcttc cggcgattta aaagggtcac
     8581 gcataccggt taaaaggcac aatgcaatac ctgccaacaa ccagcgttta accctcatgc
     8641 ggcatctcca gttgtagcgt gaacaaaaga tcatcacctt ccacgcttaa cgaaaaacgg
     8701 ctcacgctga cgttgcgctc tgccagccgt gtaaatgccg acggcactgc ttcccacagc
     8761 gttttcaacg ccaactcacc tccctgcgcg gatggatgcc aggaaaccag ttgcgcgccg
     8821 gataactgaa aatccagtgg cgaaaagggc agcgtttttt cctcgctgaa gggagtggtg
     8881 tctaccaggc gatacagtgc ggcccactgc tggtgatgac ttgcccgtag tcgaattaat
     8941 gcgtcgcgct cttcatggtg tgtcgatgat agaaaaatga gcgtaactaa catcagcaac
     9001 cagaatgccc agcaaaactg gcggaggcgg ggtgatgtgg cgaaccacca gtcaaagaac
     9061 atgttcatcg ctaaccttcc ttgttaactg atactcaaat tgccagcgtc cctgcgcatc
     9121 ctgctgcgtg gctccccgct gattgagatg aaaagacgca tcctggcgga gtgacgtttc
     9181 tagtgcgttt aacgcggtaa tgcttgttgt cagccccttg atctccagcg ttccctgctg
     9241 ccagcttatc gttgtcagcc aggcgtgctc gggtaaaagc gccgccagtg cttccagcgc
     9301 agattgccag tcgcgggtaa attgtcgctg gcgctgccgt tgcgaacgct gttcgcgtaa
     9361 ctgctgttgc tccagcaaac gtggcttcgt tatctgtaag ctgcgggcga gttgttgttc
     9421 cgcctgaagc aaaactgcgt ctatgcgcgc ttcggcgctg cctgtcagac gcagtattag
     9481 cgttatcccg acggccagca gcagaggcgc aacgaacatc agcaaccaga aacgcagaaa
     9541 agcggtccgg cgttgctgtc gccagggcaa aaaattaatt ggcggattca tcagtacgcc
     9601 ttcccaagcg ccagccccag cgcgatggca aagtctccac caggcggcgg tagcggcggc
     9661 tgacgaacag aaacggcctc ccaggggtca aatccgcctt cgccacatat cgcgacgctg
     9721 tcgggatcaa cggataacgc tgccgccagc tccttcgcgc tagtcatccc caccgccagt
     9781 ttgcgccccc agctatagcg cgtcgcccac agccactgtt cgttatcacg ccaggccaga
     9841 cattgctgat gagaaggtaa aaaaggcagg aatcgctgta atgcactggc atccggggtg
     9901 atcgcactaa catgaacacg caacctttct gccagcgtaa gcagcgttgc cagctctttg
     9961 ctttgcgcgg cagtcacgtt ataagcgggg ctgagtgagt cttcgctgta atcgaagcgc
    10021 agggagtccg gatccatatc cagctcgcgg gccatcgtcc ctgacagcca ggccgtttgc
    10081 tcccgctcac caagggacat cgacgggcgc ggaaatgacc gctgtaatgt gcgactggcg
    10141 ggaaacgcca acataatgtg atgacgctgc ggcagttcgc gactccacgg taacaacgtt
    10201 ttagccagct gctgcgcatc aacaatccgc ccatctttga taatgtcgtg cgccagcggc
    10261 aaccgccacc agcgttgcaa aaagcattct tttgcgcccc gcacgatcgc aaccgctgcc
    10321 gcttcttgct gttgtaaatg caaaccaatt tgccagatct taaatgtcat tgtgatgatc
    10381 tccttatcac ccgtcactct gacgggtata tcaatgcgtc tggcttgcct ttatactacc
    10441 gcgcgtttgt ttataaactg cccaaatgaa actaaatggg aaatttccag tgaagttcgt
    10501 aaagtatttt ttgatccttg cagtctgttg cattctgctg ggagcaggct cgatttatgg
    10561 cctataccgc tacatcgagc cacaactgcc ggatgtggtg acattaaaag atgttcgcct
    10621 gcaaattccg atgcagattt acagcgccga tggcgagctg attgctcaat acggtgagaa
    10681 acgtcgtatt ccggttacgt tggatcaaat cccaccggag atggtgaaag cctttatcgc
    10741 gacggaagac agccgcttct acgagcatca cggcgttgac ccggtgggga tcttccgtgc
    10801 ggcaagtgtg gcgctgttct ccggccacgc gtcacaaggg gcgagtacca ttacccagca
    10861 gctggcgaga aatttcttcc tcagtccaga acgcacgatg atgcgtaaga ttaaggaagt
    10921 cttcctcgcg attcgcattg aacagttgtt gacgaaagac gaaatcctcg agctttatct
    10981 gaacaagatt taccttggtt accgcgccta tggtgtcggt gctgcggcac aagtctattt
    11041 cggtaaaacg gtcgaccaac tgacgctgaa cgaaatggcg gtgatagccg ggttgccgaa
    11101 agcgccttcc acctttaatc cgctctactc aatggatcgt gccgtcgcgc ggcgtaacgt
    11161 cgtactgtcg cggatgctgg atgaagggta tatcacccag caacagttcg atcagacacg
    11221 cactgaggcg attaacgcta attatcacgc gccggagatt gctttctctg cgccgtacct
    11281 gagcgaaatg gtgcgccagg agatgtataa ccgttatggc gaaagtgcct atgaagacgg
    11341 ttatcgcatt tacaccacca tcacccgcaa agtgcagcag gccgcgcagc aggcggtacg
    11401 taataacgtg ctggactacg acatgcgcca cggctatcgc ggcccggcaa atgtgctgtg
    11461 gaaagtgggc gagtcggcgt gggataacaa caagattacc gatacgctga aggcgctgcc
    11521 gacctatggt ccgctgctgc ctgccgcagt caccagcgcc aatcctcagg gagcgacggc
    11581 gatgctggcg gacgggtcga ccgtcgcatt gagtatggaa ggcgttcgct gggcgcgtcc
    11641 ttaccgttcg gatactcagc agggaccgac gccgcgtaaa gtgaccgatg ttctgcaaac
    11701 gggtcagcaa atctgggttc gtcaggttgg cgatgcatgg tggctggcac aagtgccgga
    11761 agtgaactcg gcgctggtgt cgatcaatcc gcaaaatggt gccgttatgg cgctggtcgg
    11821 tggctttgat ttcaatcaga gcaagtttaa ccgcgccacc caggcactgc gtcaggtggg
    11881 ttccaacatc aaaccgttcc tctacaccgc ggcgatggat aaaggtctga cgctggcaag
    11941 tatgttgaac gatgtgccaa tttctcgctg ggatgcaggt gccggttctg actggcagcc
    12001 gaagaactca ccaccgcagt atgctggtcc aattcgctta cgtcaggggc tgggtcagtc
    12061 gaaaaacgtg gtgatggtac gtgcaatgcg ggcgatgggc gtcgactacg ctgcagaata
    12121 tctgcaacgc ttcggcttcc cggcgcaaaa cattgtccac accgaatcgc tggcgctggg
    12181 ttcagcgtcc ttcaccccaa tgcaggtggc gcgcggctac gcggtcatgg cgaacggcgg
    12241 cttcctagtg gacccgtggt ttatcagcaa aattgaaaac gatcagggcg gcgtgatttt
    12301 cgaagcgaaa ccgaaagtag cctgcccgga atgcgatatt ccagtgattt acggtgatac
    12361 gcagaaatcg aacgtgctgg aaaataacga tgttgaagat gtcgctatct cccgcgagca
    12421 gcagaatgtt tctgtaccaa tgccgcagct ggagcaggca aatcaggcgt tagtggcgaa
    12481 gactggcgcg caggagtacg caccgcacgt catcaacact ccgctggcat tcctgattaa
    12541 gagtgctttg aacaccaata tctttggtga accaggctgg cagggtaccg gctggcgtgc
    12601 aggtcgtgat ttgcagcgtc gcgatatcgg cgggaaaacc ggtaccacta acagttcgaa
    12661 agatgcgtgg ttctcgggtt acggtccggg cgttgtgacc tcggtctgga ttggctttga
    12721 tgatcaccgt cgtaatctcg gtcatacaac ggcttccgga gcgattaaag atcagatctc
    12781 aggttacgaa ggcggtgcca agagtgccca gcctgcatgg gacgcttata tgaaagccgt
    12841 tcttgaaggt gtgccggagc agccgctgac gccgcctccg ggtattgtga cggtgaatat
    12901 cgatcgcagc accgggcagt tagctaatgg tggcaacagc cgcgaagagt atttcatcga
    12961 aggtacgcag ccgacacaac aggcggtgca cgaggtggga acgaccatta tcgataatgg
    13021 tgaggcacag gaattgttct gattaaaaag gcgcttcggc gccttttcag ttggctgaca
    13081 aagtgcattt gtttatgccg gatgcgggtg aacgcgttat ccggctaaca aaatcgtgaa
    13141 aaatcaatat attgcaggaa ccccataagc ctgataagcg ttgtgcatca ggcaaacttc
    13201 acgcatttac actcgcccct gccctttcaa ccattcgcgc acgaggaaca gcgcgctgac
    13261 attacgcgct tcgttgaagt cagggtcttc cagcaaatcc atcatatgcg ccagcggcca
    13321 gcgcacctgt ggtagcggct ctggctcatc gccttccagc gattccgggt agagatcttg
    13381 cgcgaccacg atattcattt tgctggaaaa gtaagacggt gccatgctga gcttcttcaa
    13441 aaaagtcaga tcgttcgcac caaatccaac ctcttctttt agctcgcggt tagcggcttc
    13501 gtagacgctt tcacccggat caattaatcc tttcgaaaaa cctaattcgt aggattcagt
    13561 tcccactgcg tattcgcgga tcaggatcag gtgatcgtcc acaatcggca caatcatcac
    13621 tgcttcccgg ttggttggac gcattcgttc ataaacacgc cgcacgccat tgctgaactc
    13681 cagatccacg ctctcgacgg taaacagtcg ggaacgggct acagtttcaa cattcagaat
    13741 ggtgggtttt tgtaatgatt tgttcatcgt gggatctatg ctgtgaaatc agcggttatt
    13801 gtgcgatatc ggacacgctt tcggcaatgt gaattgcatg ttatttacat ttatgtaact
    13861 taataaataa tcgtcctcaa atcaaattaa aagtcaatag gttgaaataa ctccaggaat
    13921 ttgctgatat tccgccttca gagggtttgc tatgatcagc ggttactgtg atgtgcttaa
    13981 tgatgctcaa gttaagctcc acgcttgccg atagccaacc gcagaatcat gtattgtgtc
    14041 cggtgcgact gaccacgcct gacagactaa gtaagatggg gaaagcatga gcaccattgt
    14101 gattttttta gctgctttgc tggcctgctc actacttgcg ggatggctga taaaagtgcg
    14161 atccagacgg cgtcagctgc cctggaccaa cgccttcgcg gatgcgcaaa cgcgtaaact
    14221 cacacctgaa gaacgtagcg ccgttgaaaa ttatcttgag agcctgacgc aggtattaca
    14281 ggtgcctggc ccaacggaag ctagcgcggc accgatctct ctggcgctga atgccgaaag
    14341 caacaacgtc atgatgctga cacacgctat cacacgttac ggcatctcta ccgacgatcc
    14401 gaataaatgg cgttactacc tcgattcggt agaagtccac ctgcccccct tctgggaaca
    14461 gtacatcaac gatgagaata ccgttgaact gattcatacc gattcgctgc cgttggttat
    14521 ttcactcaac ggtcatacgc tgcaggagta catgcaggaa acgcgcggct atgccttgca
    14581 acctgttccg tccacgcagg cgtcgattcg cggggaagag agtgagcaaa tcgagctact
    14641 caatattcgc aaagaaacgc atgaagaata tgcgctgagt cgtccgcgcg ggctgcgtga
    14701 agcgttgctg atcgtcgcct ccttcctgat gttctttttc tgcctgatta ccccggatgt
    14761 atttgttccg tggctggcag gcggcgcatt actgctgctg ggcgcaggtc tgtgggggct
    14821 attcgcgccc ccggcaaaat cctccctgcg agaaattcat tgtctgcgcg gtacaccccg
    14881 tcgttgggga ttgtttggcg aaaacgatca ggaacagatc aacaatattt cactcggtat
    14941 tatcgacctg gtctatcccg cacactggca gccatacatt gctcaggatc tcggtcaaca
    15001 aaccgatatt gatatctatc tcgaccgcca cgtagtgcgt caggggcgat atctttcgct
    15061 gcatgatgaa gtaaaaaact ttccgttaca gcactggctg cgcagtacga ttatcgctgc
    15121 gggctcgctg ctagtgctgt ttatgctgtt attctggatc ccgctggata tgccgctgaa
    15181 attcactctc tcatggatga aaggcgcgca gaccattgaa gccaccagcg taaaacaact
    15241 ggctgacgct ggcgtgcggg taggcgatac attgcgtatt agcggtacgg gaatgtgtaa
    15301 tattcgcact tccggaacct ggagcgcgaa aaccaattca ccttttttac cgtttgactg
    15361 ctcgcagatc atctggaatg acgcccgctc attaccatta ccagaatctg aactggtcaa
    15421 caaagcgacg gcattgactg aagcagttaa tcgccagctg caccctaaac cggaagatga
    15481 atctcgcgtc agtgcctcat tacgttcagc gattcaaaaa tccggcatgg tattgcttga
    15541 tgattttggc gacattgtac tgaagacagc ggatttatgt tctgccaaag atgactgtgt
    15601 gcgactgaaa aatgcgctgg tcaatctcgg caacagtaaa gactgggacg cgctggtaaa
    15661 acgcgccaac gccgggaagc tcgatggcgt gaatgtgtta ttacgcccgg tgagtgctga
    15721 atcgctggat aacctggtgg caacctccac cgcgccgttt atcacgcatg aaacggcgcg
    15781 agcggcacaa tcactaaaca gtccggcccc cggcggattc ctgattgtca gcgacgaagg
    15841 cagcgatttt gttgatcagc cctggccttc ggcatcactt tacgactacc cgccgcaaga
    15901 acagtggaac gctttccaga aactggcaca aatgctgatg catacgccgt ttaacgccga
    15961 aggtatcgtc acaaaaatct tcactgacgc caatggtacg cagcatattg gccttcatcc
    16021 gatcccggat cgttccggcc tgtggcgcta tctcagcacc acattgctgc tactgacgat
    16081 gctgggtagc gccatttaca atggcgtaca ggcctggcgt cgttaccagc gtcatcgcac
    16141 tcgcatgatg gagattcagg cctattatga aagctgcctg aacccgcaac tgatcacccc
    16201 ttcagaaagc cttatcgaat aacacgtttg cgcggcaggt tatgctaccc tgtcgcgcaa
    16261 attgcttcac tctggagatt tccctcatgc atatcaacat tgcctggcag gacgtagata
    16321 ccgttctgct ggatatggac ggcacgttgc tcgacctcgc cttcgataac tatttctggc
    16381 aaaagctggt gcctgaaaca tggggcgcga aaaacggggt tacgccacag gaagcgatgg
    16441 aatatatgcg ccagcaatat cacgacgtac agcatacgct aaactggtac tgtcttgatt
    16501 actggagtga gcaactgggt ctggatatct gtgcgatgac caccgagatg ggaccgcgcg
    16561 ccgtactgcg tgaagatacc attccgtttc ttgaggcact gaaagccagc ggtaagcagc
    16621 gaattttgct caccaatgct catccgcaca acctggcggt aaaacttgag catacgggtc
    16681 tggacgcaca ccttgattta ttactttcca cccacacatt tggttatccg aaagaggatc
    16741 agcggttatg gcatgcggtg gccgaagcta cgggtctgaa agctgaaaga acgctgttta
    16801 ttgatgacag cgaagcgatt ctcgatgctg ccgcgcaatt tggtattcgt tactgcctcg
    16861 gcgtgactaa tcctgattcc gggattgccg agaaacagta tcaacgccat ccgtcactga
    16921 atgactaccg ccgcctgatc ccctcgctaa tgtgaaggag acgccatgaa agagaaacct
    16981 gctgttgagg ttcgactgga taaatggcta tgggctgccc gtttttataa aacccgcgcg
    17041 ctggcccgtg aaatgattga aggcggtaag gtgcattaca acgggcagcg cagcaagccg
    17101 agcaaaatcg tcgagctgaa tgccacgctc actctgcgcc agggaaatga cgaacgcacg
    17161 gtgattgtaa aggcgattac tgaacagcgt cgccccgcca gcgaggcagc cttgctgtat
    17221 gaagagactg cggaaagtgt agagaaacgc gaaaaaatgg cgatggcacg taaacttaat
    17281 gccttaacca tgccgcaccc ggaccgacgc ccggacaaaa aagagcgccg cgacctgtta
    17341 cgatttaaac acggcgacag tgaataactg tcacctgcaa gagagatgat tatgccgcaa
    17401 catgaccaat tacatcgcta tctgtttgaa aattttgccg ttcgcggcga actggtaacc
    17461 gtttcggaaa ccctgcaaca gatccttgag aaccacgatt atccgcagcc cgttaaaaac
    17521 gtgctggcag aactgctggt tgcgaccagc ctgttaaccg ctacgctgaa gtttgatggt
    17581 gatatcaccg tacagctgca gggcgacggt ccgatgaatc tggcggttat taacggtaac
    17641 aataaccagc aaatgcgcgg tgtggcgcgc gtgcagggcg aaattccaga aaatgccgac
    17701 ctgaaaacgc tggtcggcaa tggttacgtg gtgatcacca ttaccccgag cgaaggcgaa
    17761 cgctatcagg gcgtagttgg tctggaaggt gataccctgg cggcctgcct agaagattac
    17821 tttatgcgtt ctgaacagct gccgacgcgc ctgtttattc gcaccggcga cgtagacggc
    17881 aaaccggctg caggcggtat gttgttgcag gtaatgcctg cgcaaaatac ccaacaggac
    17941 gactttgacc acctggcgac gctaaccgaa accatcaaaa ccgaagaact gctgacctta
    18001 ccggcaaacg aagtgttgtg gcgtttgtat cacgaagaag aggtgacggt ttacgatccg
    18061 caggatgtgg agttcaaatg cacctgctcg cgtgaacgtt gcgccgatgc gctgaaaacg
    18121 ctgcctgatg aagaagttga tagcatcctg gcggaagatg gcgaaattga catgcattgt
    18181 gattactgcg gtaaccacta tctgttcaat gcgatggata ttgctgaaat ccgcaacaac
    18241 gcgtctccgg cagatccgta agttcattaa tgagtatgtc cggcagagag ggctctgccg
    18301 gattttacga cttaatggcc tgatgtgctg cgcttaccag cgcctatgaa ataaatcgca
    18361 aattattttg gtcagataca gcgtcgtatc cgataaaagc gttgtcactt acttaagttt
    18421 atttccagcg tttcaccatc taccgtcaga tcgcgccacg ctttcggcct cgtccccagt
    18481 tttccatcct tcgcagctgt gagtaatttt tctttcgtta tttcgttcgg aagtaaactc
    18541 atatcaagcg cgtcccattc ttttctgtct gagtcaaagc catagacaat gactctgtca
    18601 tcattaaaag caaacaggat ccgctccgcc tgaccatcac tattcagatc ttgctcgacc
    18661 agtacgcagg cgtctttttc aatacaggtc atcacgttat agcgatcctg gattaaggcc
    18721 gaccagaatg tcgcatccgg tttaacagaa ccaggggcaa ttaacacatt ttcggcgaat
    18781 actttttccg atacctgttc ctgaagatgt tgctctccat ctaacgccat cagcagatcc
    18841 cgcgcgcgtt tcgggtcttt cataaacccg gcatcgcttt tcagcgactc aagcgccgca
    18901 cgtccatagc gaccgctctg ttcgagcatg tagatagtta cctggtctgg cgtgtttttg
    18961 ccgctctgat aacgcgccat atgactgttc acgctaatac gcatactgtc cagcaccggc
    19021 gaattaagaa gcaccagtat caccaacacc aataacgaaa ccgcaaggtt cactttgccc
    19081 tgaagaacaa ggggattttg cccattacgc cagacgatac tgacaaaata ccccagcgac
    19141 cacaccagta acaccagctc cgccagcgcg ccctgcaagc ggtcgacagt ccagccgtac
    19201 tgagcgaccc gcagccataa cccccaggtg gcgacgaaca catacagcgg agcaaccagc
    19261 aaagcggttt taatcaggca acgcaacggc cccgtccagg gaagtgacgc tttttgcgga
    19321 tcgcggacaa tagccattaa gatcaattgc aaaaaggcca gcgttaacag caacccggcg
    19381 gcggagatgt ggcgagaaat cgcgctcagg cccgtaaacg gcagggtgat gataaacatc
    19441 agggttagca atgatactaa cggcagcaac cccgtggcga ttagcgtgaa caacttttga
    19501 atagagtcga ttaaacgtga ctgtgtacgc gcgaggatca ccgccagtgc ggtaaccaga
    19561 ccaaatgtaa gatacataaa ccagtcggtt gcaaaaaaga gcgtattgaa aaacgtgatg
    19621 ccaacaagtt taaacaactc accccacagt aaaagcacca gccacgtcag gccattggag
    19681 agaaaaatca ccagtaatat aagtacgtta tgccatactg actggtaaaa gtagctatag
    19741 cggctactgc cattgcggat acgaaggctt tgttgtatcc acggtagcaa caacattgcc
    19801 atcaacagca gatagcagcc aaaatcccaa atcgcctttt cagctctcca ggggttcatg
    19861 ccatcagttt gccacttcag ccaaccgctc atccctgtcg tggcaataaa caccagcgcc
    19921 agccatccct gtcgtggcaa taaacaccag cgccagccat ccccaaaggc gcttctgttt
    19981 aaaagagatc acggagaaga gtaaaacgga tgaaaacgcg acggtcgcag gcacgccata
    20041 aacaatccaa ctgtgatttt ttccggcaag ccaggcgata agcaggtagc aaaccagccc
    20101 ctgaagtaat ccggtagcaa tcatccccca gcgtgttgcc ggtgaaagtt caacgttatc
    20161 catgttggtt atccagaatc aaaaggtggg ttaattatcg catccgggca gtagtatttt
    20221 gcttttttca gaaaataatc aaaaaaagtt agcgtggtga atcgatactt taccggttga
    20281 atttgcatca atttcattca ggaatgcgat tccactcaca atattcccgc catataaacc
    20341 aagatttaac cttttgagaa cattttccac acctaaaatg ctatttctgc gataatagca
    20401 accgtttcgt gacaggaatc acggagtttt ttgtcaaata tgaatttctc cagatacgta
    20461 aatctatgag ccttgtcgcg gttaacaccc ccaaaaagac tttactattc aggcaataca
    20521 tattggctaa ggagcagtga aatgcgcgtt aacaatggtt tgcccccgca agaactcgag
    20581 gcttatggta tcagtgacgt acatgatatc gtttacaacc caagctacga cctgctgtat
    20641 caggaagagc tcgatccgag cctgacaggt tatgagcgcg gggtgttaac taatctgggt
    20701 gccgttgccg tcgataccgg gatcttcacc ggtcgttcac caaaagataa gtatatcgtc
    20761 cgtgacgata ccactcgcga tactttctgg tgggcagaca aaggcaaagg taagaacgac
    20821 aacaaacctc tctctccgga aacctggcag catctgaaag gcctggtgac caggcagctt
    20881 tccggcaaac gtctgttcgt tgtcgacgct ttctgtggtg cgaacccgga tactcgtctt
    20941 tccgtccgtt tcatcaccga agtggcctgg caggcgcatt ttgtcaaaaa catgtttatt
    21001 cgcccgagcg atgaagagct ggcaggtttc aaaccagact ttatcgttat gaacggcgcg
    21061 aagtgcacta acccgcagtg gaaagaacag ggtctgaact ccgaaaactt cgtggcgttt
    21121 aacctgaccg agcgcatgca gctgattggc ggcacctggt acggcggcga aatgaagaaa
    21181 gggatgttct cgatgatgaa ctacctgctg ccgctgaaag gtatcgcttc tatgcactgc
    21241 tccgccaacg ttggcgagaa aggcgatgtt gcggtgttct tcggcctttc cggcaccggt
    21301 aaaaccaccc tttccaccga cccgaaacgc cgcctgattg gcgatgacga acacggctgg
    21361 gacgatgacg gcgtgtttaa tttcgaaggc ggctgctacg caaaaactat caagctgtcg
    21421 aaagaagcgg aacctgaaat ctacaacgct atccgtcgtg atgcgctgct ggaaaacgtc
    21481 accgtgcgtg aagatggcac tatcgacttt gatgatggtt caaaaaccga gaacacccgc
    21541 gtttcttatc caatctatca catcgataac atcgttaagc cggtttccaa agcgggccac
    21601 gcgactaagg ttatcttcct gactgctgat gctttcggcg tgttgccgcc ggtttctcgc
    21661 ctgactgccg atcaaaccca gtatcacttc ctctctggct tcaccgccaa actggccggt
    21721 actgagcgtg gcatcaccga accgacgcca accttctccg cttgcttcgg cgcggcgttc
    21781 ctgtcgctgc acccgactca gtacgcagaa gtgctggtga aacgtatgca ggcggcgggc
    21841 gcgcaggctt atctggttaa cactggctgg aacggcactg gcaaacgtat ctcgattaaa
    21901 gatacccgcg ccattatcga cgccatcctc aacggttcgc tggataatgc agaaaccttc
    21961 actctgccga tgtttaacct ggcgatccca accgaactgc cgggcgtaga cacgaagatt
    22021 ctcgatccgc gtaacaccta cgcttctccg gaacagtggc aggaaaaagc cgaaaccctg
    22081 gcgaaactgt ttatcgacaa cttcgataaa tacaccgaca cccctgcggg tgccgcgctg
    22141 gtagcggctg gtccgaaact gtaatgattt gaagctggag aatatctatc cagtatctta
    22201 tagaaagcaa aacgggaggc accttcgcct cccgtttatt tacccttctt ttgtcgtgcc
    22261 ctgcgcccgc gttaccggca ctggcagcca ggcgcgaatg gaaagcccgc cccgctcgct
    22321 ggtgccaagc tccagcatcc cgttatggtt atccacgata cgctgcacaa ttgccagccc
    22381 taatcccgtg ccgctaatgg tgcgcgcact gtcgccgcgg acaaacggct ggaacaggtg
    22441 cttacgttgt tccggcgcaa ttcccggacc gtcatcttcc acctggaacc aggcgcgatt
    22501 cggctccgtt ccgctgctga ctttgatcca gccattgcca taacgggcgg cgttgaccac
    22561 catattcgcc accgcgcgtt tgatcgacag cgggtgcatt ttcacttcaa tgctgccggg
    22621 gtaaagcgcg gtttcaattt cccgctcata gccactttcg gcagcaatca cctcaccgag
    22681 cactgcatta agatccgcca tttccatcgg catctcctgc ccggtgcgca ggtagtcgat
    22741 aaactgctca atgatggcgt tgcactcttc gatatcttta ttgatcgatt ctgccagata
    22801 gccatcctgc tcgctcatca tctcagtcgc caggcgaata cgcgtcagcg gcgtgcgcaa
    22861 gtcgtgactt acccccgcca tcagcagcgt gcggtcatcc gccagttgct taacaccagc
    22921 cgccatatgg ttaaaggcac gggtaacgga acgcacctcc gaagcgccat attcacgcag
    22981 cggcggcgga ataatccctt taccaacctg caaggctgcg tgttcgagat cgaccaacgg
    23041 tcggttctgg atacgaataa acagccacgc cccgcctatc gccaatagca taatcgccag
    23101 cgtatagcgg aacagcggag agaaatcgcc ctgatgaatt tcggtcagcg gcacgcgtac
    23161 ccagatattg ggcgacagcc aggttttcag ccagacgaca ggcgaacttt tgttgacctc
    23221 aacgcgcact tccgtcgggc cgcccagttg ctgtgccatc tgatggctta agaattcata
    23281 gtgttgcgcc caacgcagac ctgcctcttc ggcagcctcg ttggagtaga gagagatccc
    23341 cagctcacgg tagatctccc gacggaaagc gggaggcaca accaactgcg tgccgtcctc
    23401 cagttgcagt ttgtcggtca tcaacatacg cacttcgtac gcgaggactt tattaaactg
    23461 ctggaggctc ggcaaaatcg cgaagttcag caccaccaga taagtcgtca ccaggctggc
    23521 gaacagcaag gtgacgatga gcaataacgt acgggcaaat gaacttcgtg gcgagaagcg
    23581 caatcgcctc atgctttaga gccgtccggt acaaagacgt agcccaggcc ccaaacggtc
    23641 tgaatgtaac gcggatgcgc tggatcttct tctaccatgc ggcgcagacg cgaaatctgc
    23701 acgtcgatgg agcgttccat tgcggaatat tcacgaccac gggcaaggtt catcagctta
    23761 tcgcgggaga gcggctcacg cggatggctg accagtgcct tcagtaccgc aaactcaccg
    23821 ctggtgagcg gcatcggctc atcttcgcgg aacatttcgc gcgtaccgag gttaagtttg
    23881 aacttaccga aagcaattac cgcctcttcc tgtgacggtg cgcctggcag ttcgttcgcc
    23941 tgacgacgca gcaccgcacg aatacgggcc agcagttcac gcgggttaaa cggttttgga
    24001 atgtagtcgt cagcgccaat ctccaggcct acgatacggt ccacttcttc ccctttcgcc
    24061 gtcaccataa tgatcggcat cgggttgctc tgactacgaa gacgtcggca aatcgacaag
    24121 ccatcttcac caggtaacat taaatccagt accataagat ggaaagattc acgagtcagc
    24181 aggcgatcca tctgttctgc attagcgacg cttcgaacct ggaagccttg ttcggtgaga
    24241 taacgttcca gcagcgcacg caggcgcatg tcgtcatcga ccaccagaat cttgtagttc
    24301 tcttgcattg tttgtactcc caaaggttcg caacaatttg taagcgtgta ttcttaaaaa
    24361 agctcacgtt cgtcaccagc taaatctggt atgaatttca gcctaaattg ttacaaagca
    24421 tattaaacag cagcttaagt atacaattta ttcggcgaaa cattattgat tctgttgata
    24481 tgatcacgtt atacccaatg tgcgcattat caaacagaca aagggaatca acgagatgaa
    24541 aacgcccctg gttacccggg aagggtatga aaaactcaaa caagagctta attatctctg
    24601 gcgtgaagaa cgcccggagg tcacaaaaaa ggtgacctgg gccgcaagtc tgggcgaccg
    24661 cagcgaaaat gctgactatc agtataataa aaagcgtctg cgtgaaatcg accgtcgcgt
    24721 gcgctatctc actaaatgcc tggaaaatct caaaatcgtc gattactccc ctcagcagga
    24781 aggcaaagtc ttttttggcg cgtgggtgga gattgaaaac gacgatggcg tgactcaccg
    24841 tttccgtatt gtcggctacg atgaaatttt tggccgtaaa gattacatct ctatcgattc
    24901 cccgatggcc cgcgcattgc tgaaaaaaga agtcggcgat ctggcggtgg tgaatacccc
    24961 tgccggggaa gcgaactggt atgttaatgc tatcgagtac gtgaaaccgt aaggaagagt
    25021 cttaacctcc tgccgatggc tggcattttt gccagccagt ccgtataact atcccctgat
    25081 ttttgatccg aaaagatgaa ctcaaaccat gatgaatgat tcgttctgcc gcattattgc
    25141 gggtgaaatt caggcgcgcc cggaacaggt tgacgctgcc gttcgcctgc ttgacgaagg
    25201 gaataccgtg ccgtttatcg cacgttatcg taaggaaatc accggcggtc tggatgacac
    25261 gcagctgcgt aatctggaaa cgcgtctgag ctatctgcgc gagctggaag agagacgtca
    25321 ggcgatcctc aaatccattt ccgagcaagg caaactcacc gatgatctgg cgaaggccat
    25381 caacgccacc ctaagcaaaa ccgaactcga agacctctac ctgccctaca aacctaaacg
    25441 ccgcacccgc gggcaaatcg ccattgaagc agggcttgag ccgttggctg acctgctgtg
    25501 gagcgatccg tcacacacgc cagaagtcgc cgctgcacaa tatgttgatg ccgataaagg
    25561 cgtggcagat accaaagccg cgctggatgg cgcgcgctat atcctgatgg aacggtttgc
    25621 cgaagatgcc acgctgctgg cgaaagtgcg tgattatctg tggaagaacg cgcatttggt
    25681 ttctacggtg gtgagcggta aagaagagga aggggcgaaa ttccgcgact atttcgatca
    25741 tcacgaaccg ttgtccacgg tgccttctca ccgcgcgctg gcgatgttcc gtgggcgtaa
    25801 cgaaggcgta ctccagcttt cgctgaatgc cgatccgcag ttcgatgagc cgcccaaaga
    25861 gagctattgc gagcaaatca tcatggatca ccttggcctg cgcctgaaca atgccccggc
    25921 ggatagctgg cgcaaaagcg tagtgagctg gacctggcgc atcaaggtgc tgatgcatct
    25981 ggaaaccgaa ctgatgggta ccgtgcgcga acgcgcggaa gatgaagcaa tcaacgtctt
    26041 tgcccgtaac ctgcacgatc tgctgatggc ggcccctgcc ggactgcgtg caacgatggg
    26101 cctcgatccg ggtctgcgta ccggggtaaa agtggcggtg gtcgatgcca ctggcaaact
    26161 ggtggcgacc gataccattt acccgcacac cggacaagcc gcaaaagcag cgatgaccgt
    26221 tgctgccttg tgtgaaaaac ataacgttga actggtggcg atcggcaacg gtacagcttc
    26281 ccgcgaaact gaacgtttct atctcgacgt gcagaagcag ttcccgaaag tgaccgcaca
    26341 gaaagtgatc gtcagcgaag ctggcgcgtc ggtttactca gcttccgagc tggcagcgca
    26401 ggagttcccg gatctcgacg tttcgctgcg tggtgcggta tctatcgccc gccgtttgca
    26461 ggatccgctg gcggagctgg tgaaaatcga tccgaaatct atcggcgtag gtcagtatca
    26521 gcatgacgtc agccagacgc aactggcccg caaactggat gcagtagtag aagactgcgt
    26581 aaacgccgtt ggcgtcgatc tcaacaccgc ttctgttccg ctattaaccc gcgtggcggg
    26641 cctgacgcgc atgatggcgc aaaacatcgt ggcctggcgc gatgagaacg gccagttcca
    26701 gaaccgtcag caactgctga aagtcagccg tctggggccg aaagccttcg agcagtgcgc
    26761 gggcttcctg cgcattaacc acggtgataa cccgctggac gcctctaccg ttcatccgga
    26821 agcctatccg gtggtggaac gcattctggc agcaacacag caggcactga aagatctgat
    26881 gggtaacagc agcgaactgc gtaacctgaa agcgtctgac tttactgatg aaaaattcgg
    26941 tgtgccgaca gtaactgaca tcatcaaaga gctggaaaaa ccgggtcgcg atccgcgtcc
    27001 ggaatttaaa accgctcagt ttgccgatgg cgtcgagaca atgaacgacc tgcaaccggg
    27061 tatgatcctc gaaggcgcag tgaccaacgt caccaacttt ggcgcgtttg tcgatattgg
    27121 cgtgcatcag gacggcctgg ttcacatctc ttcattgtcg aacaagtttg tggaagatcc
    27181 gcataccgtg gtgaaagcgg gcgacattgt gaaggtgaaa gtgctggaag tggatcttca
    27241 gcgtaaacgt atcgccctga ctatgcgtct ggacgagcag cctggcgaaa ccaactctcg
    27301 tcgcggcggc ggtaatgaac gcccgcaaaa caaccgcccg gcagccaaac cacgcggtcg
    27361 tgaagcgcag cctgccggta atagcgcgat gatggatgcg ctggcggcag cgatgggtaa
    27421 aaaacgttaa acgcccggac tggcccctaa acctgcgcga taaggcgttc acgccgcatc
    27481 tggcaacgaa cgctttgcct gacataaaag tgccggagaa tatctccggc acttttattc
    27541 cacagccaaa ctcacaatat actccgttaa tatttatcac ttcattaaca actgaaacct
    27601 taattaaaca ttagccagtc cgggtaactc actattcgaa ttatattttc gctgcgatat
    27661 aaccttgagc cacatcaaca ttgagtcaga ttattattca aaccaacatt cgcacacatt
    27721 ttaagtattg ctgatagaaa ccattctcat tatcattgtg ttgttgatta tttaatctct
    27781 cctttgttgg caaatcatct ggtctcatgt cgcggtcaaa cgccccatga ggtagttatc
    27841 cagttaatga gaaacaagta ggcacctatg caatacactc cagatactgc gtggaaaatc
    27901 actggctttt cccgtgaaat cagcccggca tatcgccaaa aactgctttc tctaggcatg
    27961 ttacctggct cctcttttga tgtggtgcgc gtcgctccac tcggcgaccc cattcatatc
    28021 gaaacccgtc gtgtgagcct ggtattacgc aaaaaagatc tggccttatt agaagtggaa
    28081 gcggtttcct gttaatacgg tgataacaac aatgaaaaaa ttaaccattg gcttaattgg
    28141 taatccaaat tctggcaaga caaccttatt taaccagctc actggcgcac gtcagcgtgt
    28201 aggtaactgg gctggcgtta ccgtcgaacg taaagaaggg caattctcca ccaccgatca
    28261 tcaggtcacg ctggtggacc tgcccggcac ctattctctg accaccatct catcgcagac
    28321 ctcgctcgat gagcaaatcg cctgtcacta cattttgagt ggcgacgccg acctgctgat
    28381 taacgtggtg gatgcgtcta accttgagcg taacctgtac ctgacgctgc aactgctgga
    28441 actcggcatt ccctgcatcg tggcgctgaa catgctcgac attgccgaga agcaaaatat
    28501 tcgtattgaa attgatgctc tatcggcacg tcttggctgc ccggtgatcc cgctggtttc
    28561 aacccgtggt cgcggtattg aagcgctcaa gctggcgatt gatcgctata aagctaacga
    28621 gaatgtggaa ctggtgcatt acgcacagcc gctactcaac gaagcagatt cactggcaaa
    28681 agtgatgcct tccgacatcc cgctgaaaca acgtcgctgg ctgggcctgc aaatgctgga
    28741 aggcgatatc tacagccgcg cctacgccgg tgaagcgtcg cagcatctgg atgccaccct
    28801 cgcccgtctg cgtaatgaaa tggatgatcc ggcgctgcac attgccgatg cgcgttacca
    28861 gtgcattgct gccatctgtg atgtggtaag caacaccctg acggcagaac ccagccgttt
    28921 caccactgcg gtagataaaa tcgtgctcaa ccgtttcctc ggtctgccga ttttcctctt
    28981 tgtgatgtac ctgatgttcc tgctggctat caacatcggc ggggcgttac agccgctgtt
    29041 taacgtcggc tccgtggcgc tatttgtgca tggtattcaa tggattggct acacgctcca
    29101 cttcccggac tggctgacta tcttcctcgc ccagggcctg ggtggcggta ttaacaccgt
    29161 gctgccactg gtgccgcaga tcggcatgat gtacctgttc ctctccttcc ttgaggactc
    29221 cggctatatg gcgcgtgcag cgtttgtgat ggaccgcctg atgcaggcgc tgggcttgcc
    29281 ggggaaatcc tttgtgccgc tgatcgtcgg tttcggttgt aacgtaccgt cggtaatggg
    29341 cgcacgtacg cttgatgcac cgcgtgaacg tctgatgacc atcatgatgg caccgtttat
    29401 gtcctgcggc gcgcgtctgg ctatcttcgc agtattcgcg gctgccttct tcgggcagaa
    29461 cggtgccctg gcggttttct cgctgtatat gctcggtatc gtgatggcgg tgctgactgg
    29521 cctgatgctc aagtacacca tcatgcgcgg tgaagcgacg ccgtttgtca tggagctgcc
    29581 ggtctaccat gtaccacacg ttaaaagcct gattatccag acctggcagc gtctgaaggg
    29641 cttcgttctg cgtgctggta aagtgattat catcgtcagc attttcctga gcgctttcaa
    29701 cagcttctcg ttgagcggga aaatcgtcga taacatcaac gactcggcgc tggcttccgt
    29761 cagccgggtg ataacccctg tcttcaagcc gattggcgta catgaagata actggcaggc
    29821 aacggttggc ctgtttacgg gcgcaatggc aaaagaagtg gtggtgggta cgctcaacac
    29881 cctctacacc gcagaaaata ttcaggacga agagttcaat ccggcggaat tcaacctcgg
    29941 tgaagagctg ttcagtgcgg tagatgaaac ctggcagagc ctgaaagaca ccttcagcct
    30001 tagcgtactg atgaacccca ttgaagctag caaaggcaac ggtgaaatgg gtaccggggc
    30061 gatgggcgtg atggatcaga aattcggtag cgcagctgcc gcatacagct acctgatttt
    30121 cgtcctgctg tatgtaccat gtatctcggt gatgggagcc atcgcccgtg aatcaagccg
    30181 tggctggatg ggcttctcca tcctgtgggg gctgaatatc gcttactcac tggcaacatt
    30241 gttctatcag gtcgccagct acagtcagca cccaacttac agcctggtgt gcattctggc
    30301 ggtaatcctg tttaacatcg tagctatcgg tctgctgcgc cgcgcgcgta gccgggtgga
    30361 tatcgaactg ctggcaaccc gcaagtcggt aagcagttgc tgcgcagcca gcaccaccgg
    30421 tgattgccat taatggcttc acttattcag gtacgtgatt tgctggcgtt acggggccgt
    30481 atggaagcga cccagataag ccagacattg aacactccgc agccaatgat taacgccatg
    30541 ctgcaacagc tggaaagcat gggcaaagcc gtgcggattc aggaagaacc tgacggctgc
    30601 ctctctggca gttgtaaaag ctgcccggaa ggcaaagcct gtctgcgcga gtggtgggcg
    30661 ctgcgttaac catactccat cgcctgtttt gaaaagcccg gtatgcgttt gcatccgggc
    30721 ttttttgcgt gcggctttcc ataaaaatgc aattcttgca gcgcaacgta agttcctctg
    30781 aaagcgtctc gcagggatga aaactcgcta atgcacagat gtggagtggc gcgtagagtc
    30841 gcggcattca aacaacaggt gaaggaacgc catgagtaaa aagcagagtt ccaccccaca
    30901 tgatgcgctg ttcaaactct ttttacgcca accggagacg gcacgcgatt ttcttgcgtt
    30961 tcatttaccg gcacccattc acgcgctctg tgatatgaaa accctcaagc tggagtcgag
    31021 gagctttatt gatgacgatc tgcgtgaaag ctattccgat gtgctgtggt cggtgaaaac
    31081 ggaacaagga ccaggataca tctattgtct gattgaacat caaagcacct caaacaaact
    31141 gatcgcattt cgcatgatgc gttacgctat tgccgcaatg caaaatcacc ttgatgctgg
    31201 atacaaaacg ttgccgatgg tggtgccatt gttgttttac cacggtattg aaagccccta
    31261 tccctattcg ctgtgttggc tggattgttt cgccgatccc aaactggcaa ggcagcttta
    31321 tgcctccgca tttccgctga ttgatgtcac cgtcatgcct gatgatgaaa tcatgcagca
    31381 ccgacgtatg gcgctgctgg agttaattca aaaacatatt cgtcaacgcg atctgatggg
    31441 gctggtagag caaatggcct gcttattaag tagtggatac gctaatgaca gacaaatcaa
    31501 agggctgttt aattacatac tgcaaactgg cgacgctgta cgttttaacg attttatcga
    31561 cggcgttgcc gaacgttcac cgaaacacaa ggagagttta atgactattg cggaaagatt
    31621 gcggcaggag ggggaacaat ccaaagccct gcatatagcc aaaataatgc ttgaatccgg
    31681 agtccctctt gcagacatca tgcgatttac cgggctgtca gaagaagagt tggctgcggc
    31741 gagtagactg gccccctgaa tctccagaca accagtatca cttaa
//
LOCUS       DAZSIO010000029        31187 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZSIO010000029 DAZSIO010000000
VERSION     DAZSIO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.029"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            389..2047
                     /locus_tag="ACHX5T_001232"
     CDS             389..2047
                     /locus_tag="ACHX5T_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR8466503.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="ACHX5T_001233"
     CDS             complement(2044..3000)
                     /locus_tag="ACHX5T_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR8466504.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="ACHX5T_001234"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="ACHX5T_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR8466505.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="ACHX5T_001235"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="ACHX5T_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR8466506.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="ACHX5T_001236"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="ACHX5T_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR8466507.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6656..6730
                     /gene="yljB"
                     /locus_tag="ACHX5T_001237"
     CDS             6656..6730
                     /gene="yljB"
                     /locus_tag="ACHX5T_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR8466508.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="ACHX5T_001238"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="ACHX5T_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR8466509.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="ACHX5T_001239"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="ACHX5T_001239"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR8466510.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9859..9946)
                     /locus_tag="ACHX5T_001240"
     tRNA            complement(9859..9946)
                     /locus_tag="ACHX5T_001240"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Ser,seq:gga)
     gene            complement(10200..10418)
                     /gene="infA"
                     /locus_tag="ACHX5T_001241"
     CDS             complement(10200..10418)
                     /gene="infA"
                     /locus_tag="ACHX5T_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR8466511.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10703..11407)
                     /gene="aat"
                     /locus_tag="ACHX5T_001242"
     CDS             complement(10703..11407)
                     /gene="aat"
                     /locus_tag="ACHX5T_001242"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR8466512.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="ACHX5T_001243"
     CDS             complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="ACHX5T_001243"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR8466513.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="ACHX5T_001244"
     CDS             complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="ACHX5T_001244"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR8466514.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="ACHX5T_001245"
     CDS             complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="ACHX5T_001245"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR8466515.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16569..17063
                     /gene="lrp"
                     /locus_tag="ACHX5T_001246"
     CDS             16569..17063
                     /gene="lrp"
                     /locus_tag="ACHX5T_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR8466516.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17198..21226
                     /gene="ftsK"
                     /locus_tag="ACHX5T_001247"
     CDS             17198..21226
                     /gene="ftsK"
                     /locus_tag="ACHX5T_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR8466517.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21381..21992
                     /gene="lolA"
                     /locus_tag="ACHX5T_001248"
     CDS             21381..21992
                     /gene="lolA"
                     /locus_tag="ACHX5T_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR8466518.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22003..23346
                     /gene="rarA"
                     /locus_tag="ACHX5T_001249"
                     /gene_synonym="mgsA"
     CDS             22003..23346
                     /gene="rarA"
                     /locus_tag="ACHX5T_001249"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR8466519.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23437..24729
                     /gene="serS"
                     /locus_tag="ACHX5T_001250"
     CDS             23437..24729
                     /gene="serS"
                     /locus_tag="ACHX5T_001250"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR8466520.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24969..27412
                     /gene="dmsA"
                     /locus_tag="ACHX5T_001251"
                     /pseudo
     CDS             24969..27412
                     /gene="dmsA"
                     /locus_tag="ACHX5T_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27423..28040
                     /gene="dmsB"
                     /locus_tag="ACHX5T_001252"
     CDS             27423..28040
                     /gene="dmsB"
                     /locus_tag="ACHX5T_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR8466521.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28042..28833
                     /locus_tag="ACHX5T_001253"
     CDS             28042..28833
                     /locus_tag="ACHX5T_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009390 - dimethyl sulfoxide reductase
                     complex [Evidence IEA];
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HFR8466522.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28869..29495)
                     /gene="ycaC"
                     /locus_tag="ACHX5T_001254"
     CDS             complement(28869..29495)
                     /gene="ycaC"
                     /locus_tag="ACHX5T_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR8466523.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29810..30958
                     /locus_tag="ACHX5T_001255"
     CDS             29810..30958
                     /locus_tag="ACHX5T_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8466524.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            30976..>31187
                     /locus_tag="ACHX5T_001256"
     CDS             30976..>31187
                     /locus_tag="ACHX5T_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR8466525.1"
                     /translation="MNLLFHTLLFCFFYLTCFFPNFIRLKIKCAAGYNFAFRYFRTSY
                     LPRIIPRFTDAPLILAKDLSMAGNVQ"
BASE COUNT         7690 a         7643 c         8374 g         7480 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgt cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcaggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
     9721 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9781 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9841 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9901 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9961 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
    10021 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10081 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10141 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10201 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10261 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10321 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10381 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10441 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10501 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10561 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10621 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10681 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10741 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10801 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10861 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10921 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10981 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    11041 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11101 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11161 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11221 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    11281 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11341 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11401 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11461 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11521 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11581 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11641 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11701 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11761 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11821 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11881 cagtgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11941 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    12001 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12061 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12121 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12181 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12241 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12301 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12361 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12421 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12481 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12541 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12601 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12661 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12721 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12781 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12841 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12901 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12961 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    13021 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13081 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13141 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13201 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13261 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13321 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13381 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13441 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13501 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13561 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13621 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13681 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13741 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13801 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13861 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13921 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13981 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    14041 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14101 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14161 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14221 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14281 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14341 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14401 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14461 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14521 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14581 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14641 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14701 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14761 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14821 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14881 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14941 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    15001 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15061 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15121 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15181 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15241 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15301 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15361 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15421 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15481 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15541 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15601 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15661 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15721 ccgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15781 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15841 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15901 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15961 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    16021 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16081 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16141 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16201 taaatgatga ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg
    16261 ttttgctttg acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16321 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16381 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16441 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16501 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16561 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16621 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16681 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16741 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16801 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16861 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16921 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16981 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    17041 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17101 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17161 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17221 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17281 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17341 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17401 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17461 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17521 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17581 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17641 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17701 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17761 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17821 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17881 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17941 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    18001 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18061 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18121 tccattatta aacagtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18181 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18241 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18301 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18361 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18421 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18481 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18541 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18601 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18661 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18721 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18781 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18841 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt
    18901 tgccgcgcca gtcttcagtc tggcaaatag cggtggaccg cgtccgcagg tcaaagaggg
    18961 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    19021 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19081 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19141 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19201 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19261 tcagtttgcg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19321 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19381 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc
    19441 acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagcagcagt atcagcagcc
    19501 acaacagcag gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca
    19561 gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca
    19621 acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc
    19681 gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa
    19741 accgacgacg ccactgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc
    19801 ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt
    19861 ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact
    19921 taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg
    19981 ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg
    20041 tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc
    20101 caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga
    20161 gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc
    20221 cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga
    20281 agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat
    20341 tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg
    20401 gtgtgttaac gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct
    20461 ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc
    20521 gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata
    20581 cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga
    20641 agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc
    20701 aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg
    20761 tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc
    20821 ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt
    20881 acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa
    20941 agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg
    21001 tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt
    21061 tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta
    21121 taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg
    21181 gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg
    21241 gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc
    21301 tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg
    21361 acgtaatttg aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct
    21421 tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag
    21481 tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc
    21541 aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac
    21601 aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg
    21661 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga
    21721 ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact
    21781 ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac
    21841 gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc
    21901 agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc
    21961 aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc
    22021 gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca
    22081 cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa
    22141 gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc
    22201 gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct
    22261 ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc
    22321 cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt
    22381 ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg
    22441 tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt
    22501 acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt
    22561 ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc
    22621 gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat
    22681 agcggtaagc gggtcctgaa gcctgaatta ctgaccgaaa tcgccggtga acgtagcgcc
    22741 cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta
    22801 cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc
    22861 gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc
    22921 gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg
    22981 gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc
    23041 aacgctgtct acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg cccggattat
    23101 gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg
    23161 caggaatatc gttatgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc
    23221 ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag
    23281 attggtgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac
    23341 cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc
    23401 acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt
    23461 aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat
    23521 aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa
    23581 gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc
    23641 gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag
    23701 ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca
    23761 gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggtacc
    23821 ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat gcactctggc
    23881 ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag
    23941 attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat
    24001 ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt
    24061 cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac
    24121 accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt
    24181 gaaatcatcg atgaagatga tctgccaatt aagatgaccg cccacacccc atgcttccgt
    24241 tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac
    24301 aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa tggcggcgct ggaagagatg
    24361 actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt
    24421 tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg
    24481 gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt
    24541 cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg
    24601 aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag
    24661 gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat
    24721 attggctaat acccaatttt tctgaatcta aaaagcgcct ttagggcgct ttttttgtct
    24781 ccctttgata ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa
    24841 atctacctat ctctttgatt ttcaaattat tctatgtata caagcctata tagcgagctg
    24901 ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgatc aagcttgagt
    24961 gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggag agtcgccgtg
    25021 gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac
    25081 cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg
    25141 ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg
    25201 tggacggtga aatcaaatat gtcgaaacga caataccggc gatgacaatt acgacggcct
    25261 acactaggtt cgcgcctgcc tgcgtgggcg ttccatgcgt caccgtgtct acaatccgga
    25321 ccgcctgaaa tatccgatga aacgagtcgg ggcgcgcggt gaaggcaaat tcgagcgcat
    25381 tagctgggaa gaagcctacg acatcatcgc gaccaatatg cagcgcctga tcaaagagta
    25441 cggcaacgag tctatctatc tgaactatgg caccggtacg ctgggcggca ccatgacccg
    25501 ctcctggccg ccgggaaata ccctggtcgc gcgcctgatg aactgctgcg gcggttatct
    25561 taaccattac ggtgactact cttccgcgca aattgcggaa ggtttgaacc atacctacgg
    25621 cggctgggca gatggtaaca gcccgtcgga tatcgaaaac agtaaattgg tagtgctgtt
    25681 tggtaataac ccaggcgaaa cgcgaatgag tggcggtggg gtgacttact atcttgaaca
    25741 ggcacgccag aaatctaatg ctcgcatgat catcatcgat ccgcgctata ccgacaccgg
    25801 tgccgggcgc gaagatgagt ggatccctat tcgtccagga acagatgccg cactggttaa
    25861 cggtctggcg tacgtcatga tcactaaaaa cctggtggat caggcattcc tcgataaata
    25921 ttgcgttggc tatgacgaga aaaccctgcc cgccagtgcg ccgaaaaatg gtcattataa
    25981 agcttatatt ctgggtgaag ggccagatgg cgtggctaaa acaccggaat gggcctcgca
    26041 aatcaccggt gttccggcag acaaaatcat caaactggct cgtgaaatcg gcagtaccaa
    26101 accggtgttt atcagccagg gatggggccc gcagcgtcac gctaacggtg aaatcgcaac
    26161 ccgtgctatc tcgatgctgg cgattctgac cggtaacgtt ggtattaatg gagggaacag
    26221 cggcgcgcgt gaaggttcat acagcttacc gtttgtccgt atgccgacct tggaaaaccc
    26281 gatccagacc agcatttcga tgtttatgtg gaccgatgcc attgaacgtg gcccggaaat
    26341 gacggcgctg cgtgatggtg tgcgcgggaa agataagctg gatgtgccga tcaaaatgat
    26401 ctggaactat gccggtaact gcctgattaa ccagcattct gaaatcaacc gtacccatga
    26461 aatcctccag gatgataaga agtgcgagct gattgtggtt atcgactgcc acatgacctc
    26521 atcggcgaaa tatgctgaca tcctgctgcc tgactgcacc gcttccgaac agatggactt
    26581 tgcactggat gcatcctgcg ggaatatgtc ttacgtgatt ttcaacgatc aggtgattaa
    26641 accgcgcttt gaatgtaaga ccatctatga aatgaccagc gaactggcaa aacgtcttgg
    26701 cgttgagcaa cagtttactg aaggccgtac ccaggaagag tggatgcggc atctgtatgc
    26761 ccagtcgcgg gaagcgattc ctgaactgcc aacgtttgaa gagttccgca agcaggggat
    26821 ctttaaaaag cgcgacccac aagggcatca cgttgcttat aaagccttcc gtgaagatcc
    26881 gcaggcaaac ccactgacta cgccatcggg caaaattgag atttattcgc aggcgctggc
    26941 tgacattgcc gctacctggg aattgccaga aggcgatgtg atcgatccac tgccgatcta
    27001 cacgccgggc tttgaaagtt atcaggatcc gctgaacaaa cagtatccgc tgcagcttac
    27061 aggtttccac tataaatctc gcgttcactc aacttacggc aacgttgatg tgctgaaagc
    27121 ggcttgccgt caggaaatgt ggatcaaccc gcttgatgcc caaaaacgcg gtatccacaa
    27181 cggcgataaa gtcaggatct ttaacgatcg tggtgaggtt catattgagg cgaaagtgac
    27241 gccacgaatg atgccgggcg tggtcgcact gggtgaaggc gcctggtatg acccggatgc
    27301 aaaacgtgtc gataagggcg gttgtatcaa cgtactgacc actcaacgtc cgtctcctct
    27361 cgctaagggg aatccgtcac atacaaacct tgttcaggtt gaaaaggtgt aaggagtaac
    27421 cgatgacaac ccagtatgga ttttttattg attccagccg ttgcaccggt tgcaaaacct
    27481 gcgagctggc ttgtaaagac tacaaagatt tgacgccaga agtcagtttc cgccgcattt
    27541 atgaatatgc tggcggcgac tggcaggaag ataacggtgt ctggcaccag aacgtgtttg
    27601 cctactatct gtcgatttcg tgcaaccact gcgaagatcc ggcttgtact aaagtctgcc
    27661 cgagcggtgc gatgcataaa cgcgaagatg gttttgttgt ggtcgatgaa gatgtatgca
    27721 ttggctgccg ttactgtcat atggcttgcc cgtatggcgc accgcaatat aacgaaacga
    27781 aaggccatat gaccaaatgc gatggttgtt atgaccgtgt tgcggagggt aaaaagccga
    27841 tctgtgttga atcttgcccg ctgcgggcgc tggatttcgg ccctatcgac gagctgcgta
    27901 aaaaacatgg cgatctggcg gccgttgcgc cgttgccgcg agctcacttt accaaaccga
    27961 atattgtgat caaacccaat gccaatagcc gcccgaccgg ggataccact ggctatctgg
    28021 caaacccgaa ggaggtgtga gatgggaagt ggatggcatg aatggccgct gatgatcttc
    28081 acggtcttcg ggcaatgtgt ggcgggtggt tttatcgttc tggctttggc gctgctcaaa
    28141 ggcgacctgc gagcagaagc ccagcagcgt gttatcgcct gcatgtttgg cttatgggta
    28201 ctgatgggca ttggctttat cgcctctatg ctccatcttg gttcaccgat gcgcgccttt
    28261 aactcgctca accgggtagg ggcttcagca ctcagtaacg aaatcgccag cggttcgatc
    28321 ttttttgctg taggcggcat cggctggttg ctggcaatgc tgaaaaagct gtcaccggca
    28381 ttgcgtacgc tatggctggt ggtgacgatg gttcttggcg tcgtctttgt ctggatgatg
    28441 gtgcgtgtgt ataacagcat tgataccgtt ccgacctggt acagcatctg gacgccgatg
    28501 ggctgggcga tgcgtctgct gccagccatt tctgtactgg cactggtggt gagtggcgtg
    28561 atgtcagtga tgcagggcac agagctggca actattcata gttctgtgca gcaggccgca
    28621 gcgctggtgc cggactatgg tgcgctgatg tcctggcgga tcgtgctttt ggccgttgcc
    28681 ctgtgcttgt ggattgcacc acagctaaaa ggttatcagc ctgcggttcc gctactatcc
    28741 gtctcattca ttctgcttct ggcaggggaa ttaatcggtc gcggcgtatt ctatggtttg
    28801 catatgaccg tggggatggc cgtcgcaagc taatcagtaa caaccggggt ttcggcctcg
    28861 gttctctttt atttctgctt cgttaacgtg tcataactgg tcatcaaatt acgataatca
    28921 ggaatatggt tagagaacaa cgtcgccagt ccttcaatat cattacgcca gtcacggtgc
    28981 agctcgcaag ccacgccgaa ccatgtcatc aattgcgcac cagcttgcga catgcgatcc
    29041 cacgccgaat gccgggtaat ttcattaaag gtccctgatg catcagtcac gacaaagaca
    29101 tcaaaccctt cttcaatggc tgaaagcgcc gggaatgcca cgcaaacttc ggttaccaca
    29161 ccggcaataa ttaactgttt tttacctgtc gctttgactg cttttacaaa atcttcgtta
    29221 tcccaggcgt taatattgcc cgggcgagca atgtaaggtg catcggggaa ttgtgctttc
    29281 agttctagaa ctaatgggcc gttaggaccg gtttcaaaac tggtggtaag aatagttggt
    29341 aagttgaaat acttagccag gtcacccagc gccagcacgt tatttttaaa cttatcgggt
    29401 tcgatatccc gtacaaggga aagtaaacca gcctggtgat caacaagcaa aacggcagca
    29461 tcatttttat caagacgaac atacggtttg gtcatcctct tctcctttcg agagtcagtg
    29521 gtacgtctga aatcggtcag acgcgatgca ttgctctgaa agcatagacg ggaaatatga
    29581 gtttgctgtg accatgaaat ttttcgactg aacgaagcgt atgaaatttg tgttagatca
    29641 ataaaaacaa tcagatgaga ttaatcatct cttcagcatt agcgttatga atgagtaata
    29701 taaatcgata ataccgccgc tgcgatatgc gcgtattttc acccattgac aatgtttttg
    29761 gcggtggcat gatgcgcatg aaatttgaac ttcctcacgg ttttaattca tgtccacgta
    29821 tacccggcct gtcatgcttt tgctgtctgg cctgcttttg ttgactctgg cgatcgcggt
    29881 gttaaataca ctcgtgccgc tttggctcgc ccaggaacac atgtccacat ggcaggtagg
    29941 cgttgtcagc tcatcctatt ttaccggcaa ccttgtcggt acattgctga cagggtatgt
    30001 cattaaacgc attggcttta accgcagcta ttatctgtcc tccttcattt ttgctgctgg
    30061 ctgtgccggc cttggcctga tgattggttt ctggagctgg ttggcgtggc gttttgtcgc
    30121 gggcgtcggc tgtgccatga tttgggtggt tgttgagagc gcgctgatgt gcagtgggac
    30181 gtcacgtaac cgtgggcgtt tgcttgctgc gtatatgatg gtttattacg tgggaacgtt
    30241 tttaggccag ttactggtca gcaaagtctc aaccgagctg atgagcgtat tgccgtgggt
    30301 tacaggtttg acattggcag ggatcttacc gctgttgttt acgcgtgtcc tgaatcagca
    30361 ggctgaaaac catgattcga cgtcaattac ggcaatgcta aaactccgtc aggcgcggct
    30421 tggcgtgaat ggctgcatta tctcaggaat cgttctggga tctctatatg gcctgatgcc
    30481 tctgtacctc aatcacaaag gggtgagcaa tgccagtatt ggtttctgga tggcggtact
    30541 ggtcagtgcg ggtatcctcg gacaatggcc gattggacgt ctggcggata agtttggtcg
    30601 actgctggtt ttgcgtgttc aggtctttgt cgtcattctc ggcagtattg cgatgcttag
    30661 ccaggcggcg atggccccag cgttattcat cctcggtgcc gctggcttta cgctatatcc
    30721 ggtggcgatg gcctgggctt gtgagaaagt tgaacatcat caactggtgg cgatgaacca
    30781 agccttactg ttgagctata ccgtgggaag tctgcttggc ccgtcattta gcgctatgct
    30841 aatgcagaat ttctccgata atttattgtt tatcatgatc gccagcgtat cgtttatcta
    30901 tttgctgatg ctgcttcgca acgccggtca tacgccgaaa cccgttgctc acgtgtaaat
    30961 gaattcaagc aaagtgtgaa cttactgttt cacactctgc ttttttgttt cttctatctg
    31021 acttgctttt ttccaaattt tattcgttta aaaataaaat gtgcagcagg ttataatttt
    31081 gcatttcgct atttccgcac ttcttatttg ccgcgcataa tccctcgttt tacagatgcc
    31141 cctttaattt tggcgaagga tttgtctatg gctgggaatg ttcagga
//
LOCUS       DAZSIO010000030        30176 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZSIO010000030 DAZSIO010000000
VERSION     DAZSIO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.030"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..182
                     /gene="tuf"
                     /locus_tag="ACHX5T_001257"
     CDS             <1..182
                     /gene="tuf"
                     /locus_tag="ACHX5T_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFR8466526.1"
                     /translation="TDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIRE
                     GGRTVGAGVVAKVLS"
     gene            412..795
                     /gene="secE"
                     /locus_tag="ACHX5T_001258"
     CDS             412..795
                     /gene="secE"
                     /locus_tag="ACHX5T_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR8466527.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            797..1342
                     /gene="nusG"
                     /locus_tag="ACHX5T_001259"
     CDS             797..1342
                     /gene="nusG"
                     /locus_tag="ACHX5T_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR8466528.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1501..1929
                     /gene="rplK"
                     /locus_tag="ACHX5T_001260"
     CDS             1501..1929
                     /gene="rplK"
                     /locus_tag="ACHX5T_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR8466529.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1933..2637
                     /gene="rplA"
                     /locus_tag="ACHX5T_001261"
     CDS             1933..2637
                     /gene="rplA"
                     /locus_tag="ACHX5T_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR8466530.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3050..3547
                     /gene="rplJ"
                     /locus_tag="ACHX5T_001262"
     CDS             3050..3547
                     /gene="rplJ"
                     /locus_tag="ACHX5T_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR8466531.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3614..3979
                     /gene="rplL"
                     /locus_tag="ACHX5T_001263"
     CDS             3614..3979
                     /gene="rplL"
                     /locus_tag="ACHX5T_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR8466532.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4299..8327
                     /gene="rpoB"
                     /locus_tag="ACHX5T_001264"
     CDS             4299..8327
                     /gene="rpoB"
                     /locus_tag="ACHX5T_001264"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR8466533.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8404..12627
                     /gene="rpoC"
                     /locus_tag="ACHX5T_001265"
     CDS             8404..12627
                     /gene="rpoC"
                     /locus_tag="ACHX5T_001265"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR8466534.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12869..14002)
                     /gene="thiH"
                     /locus_tag="ACHX5T_001266"
     CDS             complement(12869..14002)
                     /gene="thiH"
                     /locus_tag="ACHX5T_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR8466535.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13999..14769)
                     /gene="thiG"
                     /locus_tag="ACHX5T_001267"
     CDS             complement(13999..14769)
                     /gene="thiG"
                     /locus_tag="ACHX5T_001267"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR8466536.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14771..14971)
                     /gene="thiS"
                     /locus_tag="ACHX5T_001268"
     CDS             complement(14771..14971)
                     /gene="thiS"
                     /locus_tag="ACHX5T_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR8466537.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14955..15710)
                     /gene="thiF"
                     /locus_tag="ACHX5T_001269"
     CDS             complement(14955..15710)
                     /gene="thiF"
                     /locus_tag="ACHX5T_001269"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR8466538.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15703..16338)
                     /gene="thiE"
                     /locus_tag="ACHX5T_001270"
     CDS             complement(15703..16338)
                     /gene="thiE"
                     /locus_tag="ACHX5T_001270"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR8466539.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16338..18233)
                     /gene="thiC"
                     /locus_tag="ACHX5T_001271"
     CDS             complement(16338..18233)
                     /gene="thiC"
                     /locus_tag="ACHX5T_001271"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR8466540.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18314..18414)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18467..18943)
                     /gene="rsd"
                     /locus_tag="ACHX5T_001272"
     CDS             complement(18467..18943)
                     /gene="rsd"
                     /locus_tag="ACHX5T_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFR8466541.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19038..19811
                     /gene="nudC"
                     /locus_tag="ACHX5T_001273"
     CDS             19038..19811
                     /gene="nudC"
                     /locus_tag="ACHX5T_001273"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR8466542.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19851..20915
                     /gene="hemE"
                     /locus_tag="ACHX5T_001274"
     CDS             19851..20915
                     /gene="hemE"
                     /locus_tag="ACHX5T_001274"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR8466543.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21072..21743
                     /gene="nfi"
                     /locus_tag="ACHX5T_001275"
     CDS             21072..21743
                     /gene="nfi"
                     /locus_tag="ACHX5T_001275"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR8466544.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21786..22376
                     /locus_tag="ACHX5T_001276"
     CDS             21786..22376
                     /locus_tag="ACHX5T_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR8466545.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22563..22835
                     /gene="hupA"
                     /locus_tag="ACHX5T_001277"
     CDS             22563..22835
                     /gene="hupA"
                     /locus_tag="ACHX5T_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR8466546.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22848..23543
                     /locus_tag="ACHX5T_001278"
     CDS             22848..23543
                     /locus_tag="ACHX5T_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR8466547.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23545..23970)
                     /gene="zraP"
                     /locus_tag="ACHX5T_001279"
     CDS             complement(23545..23970)
                     /gene="zraP"
                     /locus_tag="ACHX5T_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR8466548.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24208..25584
                     /gene="zraS"
                     /locus_tag="ACHX5T_001280"
     CDS             24208..25584
                     /gene="zraS"
                     /locus_tag="ACHX5T_001280"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR8466549.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25581..26906
                     /gene="zraR"
                     /locus_tag="ACHX5T_001281"
     CDS             25581..26906
                     /gene="zraR"
                     /locus_tag="ACHX5T_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR8466550.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26903..28192)
                     /gene="purD"
                     /locus_tag="ACHX5T_001282"
     CDS             complement(26903..28192)
                     /gene="purD"
                     /locus_tag="ACHX5T_001282"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR8466551.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28204..29793)
                     /gene="purH"
                     /locus_tag="ACHX5T_001283"
     CDS             complement(28204..29793)
                     /gene="purH"
                     /locus_tag="ACHX5T_001283"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR8466552.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7063 a         7865 c         8307 g         6941 t
ORIGIN      
        1 ctactgacgt gactggtacc atcgaactgc cggaaggcgt agagatggta atgccgggcg
       61 acaacatcaa aatggttgtt accctgatcc acccgatcgc gatggacgac ggtctgcgtt
      121 tcgcaatccg tgaaggcggc cgtaccgttg gcgcgggcgt tgtagcaaaa gttctgagct
      181 aattgccgat aacatttgac gcaatgcgca ctaaaagggc atcatttgat gccctttttg
      241 cacgctttcg taccagaacc tggctcatca gtgattttct ttgtcataat cattgctgag
      301 acaggctctg ttgagggcgt ataatccgaa aagctaatac gcgtttcgat ttggtttgcc
      361 tcgcgatcgc ggggtgaaaa tgtttgtaga aaacttctga caggttggtt tatgagtgcg
      421 aataccgaag ctcaaggaag cgggcgcggc ctggaagcga tgaagtgggt cgttgtggtg
      481 gcattgctcc tggcggcgat tgtcggcaac tacctttatc gcgacattat gctgccgctg
      541 cgtgcgctgg ccgtagtaat tctgattgct gcagcgggtg gtgtcgcgct gttaacgaca
      601 aaaggtaaag ctaccattgc ttttgcccgt gaagcgcgta ccgaagtccg taaggtcatt
      661 tggccgactc gccaggaaac attgcacacc acgctgattg tggctgcggt taccgcagta
      721 atgtcactga tcctgtgggg actggatggt attctggttc gcctggtatc ctttatcact
      781 ggcctgaggt tctgagatgt ctgaagctcc taaaaagcgc tggtacgtcg ttcaggcgtt
      841 ttccggtttt gaaggccgcg tagcaacgtc gctgcgtgag catatcaaat tacacaacat
      901 ggaagatttg tttggtgaag tcatggtacc aaccgaagaa gtggttgaaa tccgtggcgg
      961 tcagcgtcgc aaaagcgaac gtaaattctt ccctggctac gtcctcgttc agatggtgat
     1021 gaacgacgcg agctggcact tggtgcgcag cgtaccgcgt gtgatgggct tcatcggcgg
     1081 tacttccgat cgtcctgcgc caatcagcga taaagaagtc gatgcgatta tgaaccgcct
     1141 gcagcaggtt ggtgataagc cgcgtccgaa aacgctgttt gaaccgggtg aaatggtccg
     1201 tgttaatgat ggtccgttcg ctgacttcaa cggtgttgtt gaagaagtgg attacgagaa
     1261 atctcgtctg aaagtgtctg tttctatctt cggtcgtgcg accccggtag agctggactt
     1321 cagccaggtt gaaaaagcct aacccagcga tcaaaaaagc ggcgatttaa tcgttgcaca
     1381 aggcgtgaga ttggaataca atttcgcgcc ttttgttttt atgggccttg cccgtaaaac
     1441 gattttttat atcacgggga gcctctcaga ggcgttatta cccaacttga ggaatttata
     1501 atggctaaga aagtacaagc ctatgtcaag ctgcaggttg cagctggtat ggctaacccg
     1561 agtccgccag taggtccggc tctgggtcag cagggcgtaa acatcatgga attctgcaaa
     1621 gcgttcaacg caaaaactga ttccatcgaa aaaggtctgc cgattccggt agtaatcacc
     1681 gtttacgctg accgttcttt cactttcgtt accaagaccc cgccggcagc agttctgctg
     1741 aaaaaagcgg caggtatcaa gtctggttcc ggtaagccga acaaagacaa agtgggtaaa
     1801 atttcccgcg ctcagctgca ggaaatcgcg cagaccaaag ctgccgacat gactggtgcc
     1861 gacattgaag cgatgactcg ctccatcgaa ggtactgcac gttccatggg cctggtagtg
     1921 gaggactaag aaatggctaa actgaccaag cgcatgcgtg ttatccgtga gaaagttgat
     1981 gcaaccaaac agtacgacat caacgaagct atcgcactgc tgaaagagct ggcgactgct
     2041 aaattcgtag aaagcgtgga cgtagctgtt aacctcggca tcgacgctcg taaatctgac
     2101 cagaacgtac gtggtgcaac tgtactgccg cacggtactg gccgttccgt tcgcgtagcc
     2161 gtatttaccc aaggtgcaaa cgctgaagct gctaaagctg caggcgcaga actggtaggt
     2221 atggaagatc tggctgacca gatcaagaaa ggcgaaatga actttgacgt tgttattgct
     2281 tctccggatg caatgcgcgt tgttggccag ctgggccagg ttctgggtcc gcgtggcctg
     2341 atgccaaacc cgaaagtggg tactgtaaca ccgaacgttg ctgaagcggt taaaaacgct
     2401 aaagctggcc aggttcgtta ccgtaacgac aaaaacggca tcatccacac caccatcggt
     2461 aaagtggact ttgacgctga caaactgaaa gaaaacctgg aagctctgct ggttgcgctg
     2521 aaaaaagcaa aaccgactca ggcgaaaggc gtgtacatca agaaagttag catctccacc
     2581 accatgggtg caggtgttgc agttgaccag gctggcctga gcgcttctgt aaactaatgc
     2641 ctttacgtgg gcggtgattt tgtctacaat cttaccccca cgtataatgc ttaatgcaga
     2701 cgtatatccg agatattcgg gttgtggcaa ggcggcaact gagtgagtcg ccaagagcat
     2761 agctaactat gtgactggtg cgaatgaagg aagccaacgc cgccacaagt tgaatagcga
     2821 cggatagaaa agatttgttc gttggagcct ggcctatcca ggcctccgtc gaagaccgca
     2881 ggagtttcgc aagaaactta atcccctgcg tagacggtga cagaacgcta agattattct
     2941 tttatattct ggcttgtttc tgctcaccgt aattaagacg ctctctccgt ttggaggagt
     3001 gaagtgagtt ccagagattt tctctggcaa acatccagga gcaaagctaa tggctttaaa
     3061 tcttcaagac aaacaagcga ttgttgctga agtcagcgaa gtagccaaag gcgcgctgtc
     3121 tgcagtagtt gcggattccc gtggcgtaac tgtagataaa atgactgaac tgcgtaaagc
     3181 aggtcgcgaa gctggcgtat acatgcgtgt tgttcgtaac accctgctgc gccgtgctgt
     3241 tgaaggtact ccgttcgagt gcctgaaaga cgcgtttgtt ggtccgaccc tgattgcata
     3301 ctctatggaa cacccgggcg ctgctgctcg tctgttcaaa gagttcgcga aagcgaatgc
     3361 aaaatttgag gtcaaagccg ctgcctttga aggtgagctg atcccggcgt ctcagatcga
     3421 ccgcctggca actctgccga cctacgaaga agcaattgca cgcctgatgg caaccatgaa
     3481 agaagcttcg gctggcaaac tggttcgtac tctggctgct gtacgcgatg cgaaagaagc
     3541 tgcttaatcg cagttatctt tttaacgcat tcgcttacgt ataaacttat tctgatattc
     3601 aggaacaatt taaatgtcta tcactaaaga tcaaatcatt gaagcagttg cagctatgtc
     3661 tgtaatggac gttgtagaac tgatctctgc aatggaagaa aaattcggtg tttccgctgc
     3721 tgctgctgta gctgtagctg ctggcccggt tgaagctgct gaagaaaaaa ctgaattcga
     3781 cgtaattctg aaagctgctg gcgctaacaa agttgctgtt atcaaagcag tacgtggcgc
     3841 aactggcctg ggtctgaaag aagctaaaga cctggtagaa tctgcaccgg ctgctctgaa
     3901 agaaggcgtg agcaaagacg acgcagaagc actgaaaaaa gctctggaag aagctggcgc
     3961 tgaagttgaa gttaaataag ccaacccttc cggttgcagc ctgagaaatc aggctgatgg
     4021 ctggtgactt tttagtcacc agcctttttg cgctgtaagg cgccagtagc gtttcacact
     4081 gtttgactac tgctgtgcct ttcaatgctt gtttctatcg acgacttaat atactgcgac
     4141 aggacgtccg ttctgtgtaa atcgcaatga aatggtttaa gcgtgatagc aacaggcatt
     4201 gcggaaagtg ttccattttc cggtcaacaa aatagtgttg cacaaactgt ccgctcaatg
     4261 gacagatggg tcgacttgtc agcgagctga ggaaccctat ggtttactcc tataccgaga
     4321 aaaaacgtat tcgtaaggat tttggtaaac gtccacaagt tctggatgta ccttatctcc
     4381 tttctatcca gcttgactcg tttcagaaat ttatcgagca agatcctgaa gggcagtatg
     4441 gtctggaagc tgctttccgt tccgtattcc cgattcagag ctacagcggt aattccgagc
     4501 tgcaatacgt cagctaccgc cttggcgaac cggtgtttga cgtccaggaa tgtcaaatcc
     4561 gtggcgtgac ctattccgca ccgctgcgcg ttaaactgcg tctggtgatc tatgagcgcg
     4621 aagcgccgga aggcaccgta aaagacatta aagaacaaga agtctacatg ggcgaaattc
     4681 cgctcatgac agacaacggt acctttgtta tcaacggtac tgagcgtgtt atcgtttccc
     4741 agctgcaccg tagtccgggc gtcttctttg actccgacaa aggtaaaacc cactcttcgg
     4801 gtaaagtgct gtataacgcg cgcatcatcc cttaccgtgg ttcctggctg gacttcgaat
     4861 tcgatccgaa ggacaacctg ttcgtacgta tcgaccgtcg ccgtaaactg cctgcgacca
     4921 tcattctgcg cgccctgaac tacaccacag agcagatcct cgacctgttc tttgaaaaag
     4981 ttatctttga aatccgtgat aacaagctgc agatggaact ggtgccggaa cgcctgcgtg
     5041 gtgaaaccgc atcttttgac atcgaagcta acggtaaagt gtacgtagaa aaaggccgcc
     5101 gtatcactgc gcgccacatt cgccagctgg aaaaagacga cgtcaaactg attgaagtcc
     5161 cggttgagta catcgcaggt aaagtggttg ctaaagacta tattgatgag tctaccggcg
     5221 agctgatctg cgcagcgaac atggagctga gcttggatct gctggctaag ctgagccagt
     5281 ctggtcacaa gcgtatcgaa acgctgttca ccaacgatct ggatcacggc ccatatatct
     5341 ctgaaacctt acgtgtcgac ccaactaacg accgtctgag cgcactggta gaaatctacc
     5401 gcatgatgcg ccctggcgag ccgccgactc gtgaagcagc tgaaagcctg ttcgagaacc
     5461 tgttcttctc cgaagaccgt tatgacttgt ctgcggttgg tcgtatgaag ttcaaccgtt
     5521 ctctgctgcg cgaagaaatc gaaggttccg gtattctgag caaagacgac atcattgatg
     5581 ttatgaaaaa gctcatcgat atccgtaacg gtaaaggcga agtcgatgat atcgaccacc
     5641 tcggcaaccg tcgtatccgt tccgttggcg aaatggcgga aaaccagttc cgcgttggcc
     5701 tggtacgtgt agagcgtgcg gtgaaagagc gtctgtctct gggcgatctg gataccctga
     5761 tgcctcagga tatgatcaac gccaagccga tttccgcagc agtgaaagag ttcttcggtt
     5821 ccagccagct gtctcagttt atggaccaga acaacccgct gtctgagatt acgcacaaac
     5881 gtcgtatctc cgcactcggc ccaggcggtc tgacccgtga acgtgcaggc ttcgaagttc
     5941 gagacgtaca cccgactcac tacggtcgcg tatgtccaat cgaaacccct gaaggtccga
     6001 acatcggtct gatcaactct ctgtccgtgt acgcacagac taacgaatac ggcttccttg
     6061 agactccgta tcgtaaagtg accgacggtg ttgtaactga cgaaattcac tacctgtctg
     6121 ctatcgaaga aggcaactac gttatcgccc aggcgaactc caacctggat gaagaaggcc
     6181 acttcgtaga agacctggta acttgccgta gcaaaggcga atccagcttg ttcagccgcg
     6241 accaggttga ctacatggac gtatccaccc agcaggtggt atccgtcggt gcgtccctga
     6301 tcccgttcct ggaacacgat gacgccaacc gtgcattgat gggtgcgaat atgcaacgtc
     6361 aggccgttcc gactctgcgc gctgataagc cgctggttgg tactggtatg gaacgtgctg
     6421 ttgccgttga ctccggtgta actgcggtag ctaaacgtgg tggtgtcgtt cagtacgtgg
     6481 atgcttcccg tatcgttatc aaagttaacg aagacgagat gtatccgggt gaagcaggta
     6541 tcgacatcta caacctgacc aaatacaccc gttctaacca gaacacctgt atcaaccaga
     6601 tgccgtgtgt gtctctgggt gaaccggttg aacgtggcga cgtgctggca gacggtccgt
     6661 ccaccgacct cggtgaactg gcgcttggtc agaacatgcg cgtagcgttc atgccgtgga
     6721 atggttacaa cttcgaagac tccatcctcg tatccgagcg tgttgttcag gaagaccgtt
     6781 tcaccaccat ccacattcag gaactggcgt gtgtgtcccg tgacaccaag ctgggtccgg
     6841 aagagatcac cgctgacatc ccgaacgtgg gtgaagctgc gctctccaaa ctggatgaat
     6901 ccggtatcgt ttacattggt gcggaagtga ccggtggcga cattctggtt ggtaaggtaa
     6961 cgccgaaagg tgaaactcag ctgaccccag aagaaaaact gctgcgtgcg atcttcggtg
     7021 agaaagcctc tgacgttaaa gactcttctc tgcgcgtacc aaacggtgta tccggtacgg
     7081 ttatcgacgt tcaggtcttt actcgcgatg gcgtagaaaa agacaaacgt gcgctggaaa
     7141 tcgaagaaat gcagctcaaa caggcgaaga aagacctgtc tgaagaactg cagatcctcg
     7201 aagcgggtct gttcagccgt atccgtgctg tgctggtagc cggtggcgtt gaagctgaga
     7261 agctcgacaa actgccgcgc gatcgctggc tggagctggg cctgacagac gaagagaaac
     7321 aaaatcagct ggaacagctg gctgagcagt atgacgaact gaaacacgag ttcgagaaga
     7381 aactcgaagc gaaacgccgc aaaatcaccc agggcgacga tctggcaccg ggcgtgctga
     7441 agattgttaa ggtatatctg gcggttaaac gccgtatcca gcctggtgac aagatggcag
     7501 gtcgtcacgg taacaagggt gtaatttcta agatcaaccc gatcgaagat atgccttacg
     7561 atgaaaacgg tacgccggta gacatcgtac tgaacccgct gggcgtaccg tctcgtatga
     7621 acatcggtca gatcctcgaa acccacctgg gtatggctgc gaaaggtatc ggcgacaaga
     7681 tcaacgccat gctgaaacag cagcaagaag tcgcgaaact gcgcgaattc atccagcgtg
     7741 cgtacgatct gggcgctgac gttcgtcaga aagttgacct gagtaccttc agcgatgaag
     7801 aagttatgcg tctggctgaa aacctgcgca aaggtatgcc aatcgcaacg ccggtgttcg
     7861 acggtgcgaa agaagcagaa attaaagagc tgctgaaact tggcgacctg ccgacttccg
     7921 gtcagatccg cctgtacgat ggtcgcactg gtgaacagtt cgagcgtccg gtaaccgttg
     7981 gttacatgta catgctgaaa ctgaaccacc tggtcgacga caagatgcac gcgcgttcca
     8041 ccggttctta cagcctggtt actcagcagc cgctgggtgg taaggcacag ttcggtggtc
     8101 agcgtttcgg ggagatggaa gtgtgggcgc tggaagcata cggcgcagca tacaccctgc
     8161 aggaaatgct cactgttaag tctgatgacg tgaacggtcg taccaagatg tataaaaaca
     8221 ttgtggacgg caaccatcag atggagccgg gcatgccaga atccttcaac gtattgttga
     8281 aagagattcg ttcgctgggt atcaacatcg aactggaaga cgagtaattc tcgctcaaac
     8341 aggtcactgc tgtcggggta aaacccggca gcggattgtg ctaactccga cgggagcaaa
     8401 tccgtgaaag atttattaaa gtttctgaaa gcgcagacta aaaccgaaga gtttgatgcg
     8461 atcaaaattg ctctggcttc gccagacatg atccgttcat ggtctttcgg tgaagttaaa
     8521 aagccggaaa ccatcaacta ccgtacgttc aaaccagaac gtgacggcct tttctgcgcc
     8581 cgtatctttg ggccggtaaa agattacgag tgcctgtgcg gtaagtacaa gcgcctgaaa
     8641 caccgtggcg tcatctgtga gaagtgcggc gttgaagtga cccagactaa agtacgccgt
     8701 gagcgtatgg gccacatcga actggcttcc ccgactgcgc acatctggtt cctgaaatcg
     8761 ctgccgtccc gtatcggtct gctgctcgat atgccgctgc gcgatatcga acgcgtactg
     8821 tactttgaat cctatgtggt tatcgaaggc ggtatgacca acctggaacg tcagcagatc
     8881 ctgactgaag agcagtatct ggacgcgctg gaagagttcg gtgacgaatt cgacgcgaag
     8941 atgggtgcgg aagcaatcca ggctctgctg aagagcatgg atctggagca agagtgcgaa
     9001 cagctgcgtg aagagctgaa cgaaaccaac tccgaaacca agcgtaaaaa gctgaccaag
     9061 cgtatcaaac tgctggaagc gttcgttcag tctggtaaca aaccagagtg gatgatcctg
     9121 accgttctgc cggtactgcc gccagatctg cgtccgctgg ttccgctgga tggtggtcgt
     9181 ttcgcgactt ctgacctgaa cgatctgtat cgtcgcgtca ttaaccgtaa caaccgtctg
     9241 aaacgtctgc tggatctggc tgcgccggac atcatcgtac gtaacgaaaa acgtatgctg
     9301 caggaagcgg tagacgccct gctggataac ggtcgtcgcg gtcgtgcgat caccggttct
     9361 aacaagcgtc ctctgaaatc tttggccgac atgatcaaag gtaaacaggg tcgtttccgt
     9421 cagaacctgc tcggtaagcg tgttgactac tccggtcgtt ctgtaatcac cgtaggtcca
     9481 tacctgcgtc tgcatcagtg cggtctgccg aagaaaatgg cgctggagct gttcaaaccg
     9541 ttcatctacg gcaagctgga actgcgtggt cttgctacca ccattaaagc tgcgaagaaa
     9601 atggttgagc gcgaagaagc tgtcgtttgg gatatcctgg acgaagttat ccgcgaacac
     9661 ccggtactgc tgaaccgtgc accgactctg caccgtctgg gtatccaggc atttgaaccg
     9721 gtactgatcg aaggtaaagc tatccagctg cacccgctgg tttgtgcggc atataacgcc
     9781 gacttcgatg gtgaccagat ggctgttcac gtaccgctga cgctggaagc ccagctggaa
     9841 gcgcgtgcgc tgatgatgtc taccaacaac atcctgtccc cggcgaacgg cgaaccaatc
     9901 atcgttccgt ctcaggacgt tgtactgggt ctgtactaca tgacccgtga ctgtgttaac
     9961 gccaaaggcg aaggcatggt gctgactggc ccgaaagaag cagaacgtct gtatcgctct
    10021 ggtctggctt ctctgcatgc gcgcgttaaa gtgcgtatca ccgagtatga aaaagatgct
    10081 aacggtgaat tagtagcgaa aaccagcctg aaagacacga ctgttggccg tgccattctg
    10141 tggatgattg taccgaaagg tctgccttac tccatcgtca accaggcgct gggtaaaaaa
    10201 gcaatctcca aaatgctgaa cacctgctac cgcattctcg gtctgaaacc gaccgttatt
    10261 tttgcggacc agatcatgta caccggcttc gcctatgcag cgcgttctgg tgcatctgtt
    10321 ggcatcgatg acatggtcat cccggagaag aaacacgaaa tcatctccga ggcagaagca
    10381 gaagttgctg aaattcagga gcagttccag tctggtctgg taactgcggg cgaacgctac
    10441 aacaaagtta tcgatatctg ggctgcggcg aacgatcgtg tatccaaagc gatgatggat
    10501 aacctgcaaa ctgaaaccgt tattaaccgt gacggtcagg aagagaagca ggtttccttc
    10561 aacagcatct acatgatggc cgactccggt gcgcgtggtt ctgcggcaca gattcgtcag
    10621 cttgctggta tgcgtggtct gatggcaaag ccggatggct ccatcatcga aacgccaatc
    10681 accgcgaact tccgtgaagg tctgaacgta ctccagtact tcatctccac ccacggtgct
    10741 cgtaaaggtc tggcggatac cgcactgaaa actgcgaact ccggttacct gactcgtcgt
    10801 ctggttgacg tggcgcagga cctggtggtt accgaagacg attgtggtac ccatgaaggt
    10861 atcatgatga ctccggttat cgaggggggt gacgttaaag agccgctgcg cgatcgcgta
    10921 ctgggtcgtg taactgctga agacgttctg aagccgggta ctgctgatat tctcgttccg
    10981 cgcaacacgc tgctgcacga acagtggtgt gacctgctgg aagagaactc tgtcgacgcg
    11041 gtaaaagtac gttctgttgt atcttgtgac accgactttg gtgtatgtgc gcactgctac
    11101 ggtcgtgacc tggcgcgtgg ccacatcatc aacaaaggtg aagcaatcgg tgttatcgcg
    11161 gcacagtcca tcggtgaacc gggtacacag ctgaccatgc gtacgttcca catcggtggt
    11221 gcggcatctc gtgcggctgc tgaatccagc atccaggtga aaaacaaagg tagcatcaag
    11281 ctcagcaacg tgaagtcggt tgtgaactcc agcggtaaac tggttatcac ttcccgtaac
    11341 accgaactga aactgatcga cgaattcggt cgtaccaaag aaagctacaa agtgccttac
    11401 ggtgcggtac tggcgaaagg cgatggcgaa caggttgctg gcggcgaaac cgttgcaaac
    11461 tgggacccgc acaccatgcc ggttatcacc gaagtaagcg gttttgtacg ctttactgac
    11521 atgatcgacg gccagaccat tactcgtcag accgacgaac tgaccggtct gtcttcgctg
    11581 gtggttctgg attccgcaga acgtaccgca ggtggtaaag atctgcgtcc ggcactgaaa
    11641 atcgttgatg ctcagggtaa cgacgttctg atcccaggta ccgatatgcc tgcgcagtac
    11701 ttcctgccgg gtaaagcgat tgttcagctg gaagatggcg tacagatcag ctctggtgac
    11761 accttggcgc gtattccgca ggaatccggc ggtaccaagg acatcaccgg tggtctgccg
    11821 cgcgttgcgg acctgttcga agctcgtcgt ccgaaagagc cggcaatcct ggctgaaatc
    11881 agcggtatcg tttccttcgg taaagaaacc aaaggtaaac gtcgtctggt tatcaccccg
    11941 gtagacggta gcgatccgta cgaagagatg attccgaaat ggcgtcagct caacgtgttc
    12001 gaaggtgaac gtgtagaacg tggtgacgta atttccgacg gtccggaagc gccgcacgac
    12061 attctgcgtc tgcgtggtgt tcatgctgtg actcgttaca tcgttaacga agtacaggac
    12121 gtataccgtc tgcagggcgt aaagattaac gataaacaca tcgaagttat cgttcgtcag
    12181 atgctgcgta aagctaccat cgttaacgcg ggcagctccg acttcctgga aggcgaacag
    12241 gttgaatact ctcgcgtcaa gatcgcaaac cgcgaactgg aagcgaacgg caaagtgggt
    12301 gcaacttact cccgcgatct gctgggtatc accaaagcgt ctctggcaac cgagtccttc
    12361 atctccgcgg catcgttcca ggagaccact cgcgtgctga ccgaagcagc cgttgcgggc
    12421 aaacgcgacg aactgcgcgg cctgaaagag aacgttatcg tgggtcgtct gatcccggca
    12481 ggtaccggtt acgcgtacca ccaggatcgt atgcgtcgcc gtgctgcggg tgaagctccg
    12541 gctgcaccgc aggtgactgc agaagacgca tctgccagcc tggcagaact gctgaacgca
    12601 ggtctgggcg gttctgataa cgagtaatcg ttaatccgca aataacgtaa aaacccgctt
    12661 cggcgggttt ttttatgggt gttattcaga gggcaaattc ttcacggaaa atattaagaa
    12721 aggcagggcg aaacgaggaa gaagcttttc gccctggaca acattcctgc tgacggcact
    12781 accataaaaa aacacaacgc tttcaacaac cttgcgtttt tcatcttccg tagcggctcg
    12841 gaataaattt acgttcttac attccgtctc atagtctttg cgaggcgcgt cccagatagc
    12901 tgtcccagtc tttccatacc ggctgcaaac cctgagcggt taacacggca gcaaccgctt
    12961 ccggtctgcg atcgtcgtgc ggtgagaact gttccaactc ggggtgatta tcggcatagc
    13021 cacctggctg cgttttcgag aaggcgctga cgttattgat cgccagcgga ataacgcgat
    13081 cgcgaaacca cggtgattcc cgcgtggaga gtgacagttc aatctccggt gcaagcagtc
    13141 ggaaggcgca gatggtttgc actaactggc gttcatccat aatcgacgca ggctcaatgc
    13201 cgccagtaca cgggcgcagg cgcggaaagg agacagagta acggctttgc cagtaatgct
    13261 gttgcagcca tagcaaatgt tcggcaacca tatagcagtc aacgcgccag ttgtcggaaa
    13321 ggccaattag cgcgccgagg cctatcttat caatccccgc acgccccagc cgatccggcg
    13381 tttccagccg ccagaagaag tcctgttttt tgcctttcaa atgatggcgg gtataagtcg
    13441 cctcgtgata tgtctcctga taaaccatca ccccatccag accaagttgc tttaactcgg
    13501 cgtattccgt ctccgccagc ggttgcactt ccatttgtag tgaagagaac tgttcacgca
    13561 gggcggggag atgacgacga aagtaatcca tccccacttt cgcctgatgt tcaccagtga
    13621 ctaatagcag atgttcaaag cccatctccc gtatagcggc gctttccctg gcaatatccg
    13681 cttcatccag cgttttgcgc ttgatgcgat tactcatgga aaatccgcag tacgtgcagt
    13741 cgttagcgca aagattggaa agataaagcg ggacgtagaa actaactgtg ttgccaaatc
    13801 gctgacgggt cagacgctgc gcctgttggg ccagttgttc cagatagcca ctggcggcag
    13861 gcgataacag cgccatcatg tcgtcgcggg tgagttgcga ggcatttagc gcccgctcta
    13921 cgtcagcagc cgttttgccg ttgatacgca ggcggatgtc gtcccagtcc agttgtcgcc
    13981 agcgattgct gaaggttttc atgccgatgc ctccagaaat ccggtcagcg ggctggtggc
    14041 atgagcaaaa taactgcggc tgcccggtcc ggactgacgt gccagcaggc ctacttctac
    14101 cgccagacga aatgccttcg ccatgttgac gggatcgtcc gcgacggcaa tcgccgtatt
    14161 cactaacacc gcgtcggccc ccatttccag cgcctgcgtg gcatggctgg gaacgccgat
    14221 gccagcatcg acaaccaccg gaactgtggc ctgctggata ataatctcca gcatggcacg
    14281 ggtttccagt ccctgattcg agccaatcgg cgcgccgagc ggcatcaccg ctgcacagcc
    14341 gacttcttcc agacgtttac acaataccgg atcggccccg cagtaaggca gcacgacaaa
    14401 tccctgttgt accagcgttt cggcggcttt cagggtttcg atgggatcgg gcaacagcca
    14461 gcgggcgtca gggtgaatct ccaattttag ccagtttgtg cctaacgctt cacgtgccag
    14521 atgggcggcg aaaatggctt cttccgctgt tttcgccccg gaagtattcg gcagcagggc
    14581 cacacccgcc gcgataagcg gttcgaggat agcgtcgttg tgctggcgca agtcgacacg
    14641 tttcatcgcc agtgtcacca gctggctgcc ggaagcgcgg atcgcctcca ccatcagttg
    14701 tgaagaagcg aatttccctg tgccggtaaa cagatgtgaa tcaaacgttt tgtccgcaat
    14761 acgtaacatc tcaaccccct gcaataacct gaaaaagcag gatctggtcg ccatcctgca
    14821 cgatatgttg cgtccactgc tcacgcggga cgatttgctg attaatcgcc agagccgcgc
    14881 ccgcttgtcg ttggtcgagt tgctccagta gttcgtgaac agtttgcccg gcggcgcact
    14941 gcatcggttg atcgttaaac aggatctgca ttgcttcctc cgcataccgg gcaaccactg
    15001 gcgcggcgca acgccaggct gcgccactgg ctcgacttac cgtcgaacag tcggagttct
    15061 cccgcaggcg tctctatacc gcttaataac ttaatggctt ccagtgcctg caaagtgccc
    15121 atcaccccga ccaccgggcc aaccacgccc gccgtgcggc agttgcgttc tggctgctgg
    15181 ttatctggcc acaggcagcg gtaacacccc tgctcccagg gcggtgtcag taccatcaac
    15241 tgaccgccaa atccgaccgc gctggcggtg ataagcggcg tgttgagtgc cacgcaggcg
    15301 gcattaatct cctggcgagt cgccatattg tcggtacagt cgagcaccac atcggcttgt
    15361 gcaaccgcat ctttcaacgc ctcacccgtt aaccgttgtt gtaatgctgt cagttgaatg
    15421 tcgggattca actgtgtcag tcgctgttgg ctgacctgcg atttcgggct gttgatatct
    15481 tcagtggtaa agaggatttg tcgttgcaga ttgcttaaat gcacatcgtc gtcatctgcc
    15541 agtaccagcg tcccgacgcc agcccctgcc aggtatagcg cagcaggtgt acccagcccg
    15601 ccaagaccaa taatcagcac ctggctgtcg agcagttttt gctgcccgtc aagagcgata
    15661 tcgtcgagca gaatttggcg gctataacgc ataaagtcac ggtcattcat cgccaactcc
    15721 tgcaatttcc agcaactgtg ccgttgccaa acgccagtct gcggcttgag taatggcgct
    15781 gacgacggcg atactgccga cacccgttgc tatcaccgca ggcgcgcgtg ccagactgat
    15841 accgccaatc gccacggtgg gataatccgc caggcgctca acatgccgtg ccaactgttc
    15901 cagcccctgc ggtgcagaag gcatctgttt ggtttgcgtc gggaacacat gtcccagcgc
    15961 gatataagag gggcgtgctg ccagcgcgac gtcgatttcc atatcgtcat gtgtcgaaac
    16021 gcccagccgc aggcctgccg cgcggatggc attgagatcg gtggcttgca aatcttcctg
    16081 ccccaaatgg acgccatacg cctgatgctt gatcgcgagc cgccagtaat cgttgataaa
    16141 caatcgcgcg ttatagcggc ggcccagcgc aattgccgcc acgacatcgg cttccacctc
    16201 ttcatcgcgc cgatctttga tgcgtagctg gagagtacgt acgcctgcat ccaacagacg
    16261 ttcgatccac tgtacgctgt ccaccaccgg gtacagtcct aaacgaaaag gtacaggagg
    16321 aaaatcaggc tgatacatca cgcttcctcc ttatgcaggt agatttctcc gcctctggca
    16381 cggaagttct ccgacatatc cgccattccc acttcaatgg tttgcgcggc ggcgtaatcg
    16441 cgtacttcct ggctgatttt catcgagcag aattttggcc cacacatgga gcaaaaatgg
    16501 gcgactttgc cggactcttg gggcagggtt tcatcgtgat aagcgcgggc ggtaaacggg
    16561 tcgagggcca gattaaactg gtcttcccag cgaaattcga agcgggcttt cgacatggcg
    16621 ttatcgcgaa tttgcgcgcc cggatgccct tttgccaggt cagcagcgtg ggcggcaatc
    16681 ttataagtga taagcccctg cttaacatct tctttattgg gcagacccag atgctctttt
    16741 ggcgttacgt aacagagcat cgcgcagcca aaccagccaa tcatcgccgc accaatcccc
    16801 gacgtgaagt ggtcatagcc cggtgcaata tcggtagtta gcggccccag agtgtaaaac
    16861 ggcgcttcgt ggcagtgctc taactcctcg gtcatattgc ggcggatcat ctgcatcggc
    16921 acatggcctg ggccttcaat catcacctgt acgtcatatt cccaggcgat tttggtcagt
    16981 tcgcccagcg tatgcaattc ggcaaactgc gcttcatcgt tggcgtcctg aatagaaccg
    17041 gggcgcagac cgtcgcccag cgacagcgaa acgtcataag cggcacagat ttcacaaatt
    17101 tcgcggaagt gttgatagag gaaattttcc tgatgatggg agaggcacca tttcgccata
    17161 atcgaaccgc cgcgagagac gataccggtc aggcgtttcg cggtcatcgg cacatagcgc
    17221 agcagtacgc ccgcatggat agtgaagtaa tccacacctt gctcggcctg ttccagcagc
    17281 gtgtcgcgga acgcttccca ggtaagatct tcggcgatcc cgttaacctt ctccagcgcc
    17341 tggtagatcg gcactgtacc gatcggcacc ggactgttac gcaaaatcca ctcgcgggtt
    17401 tcgtgaatat agcgaccggt ggagagatcc atcaccgtat ccgctcccca gcgcgtggac
    17461 cataccagct tttccacttc ttcttcgatg gaagaggtga ccgccgagtt gccgatattg
    17521 gcgttaactt ttaccaggaa gttacggcca ataatcatcg gctccgattc cggatgatta
    17581 atgttggccg ggataatcgc acgtccggca gcaacttcat cacggacaaa ttccgcagtg
    17641 atattttccg gcagatgtgc gccaaagctc attcccggat gctggtggcg taaaacctcg
    17701 ctacggatgc gctcgcggcc catattctcg cggatggcga tgaattccat ttccggcgtg
    17761 atgatgccct ggcgggcgta gtgcagttgg gtgacacggc gtcctgtttt ggcgcgtttt
    17821 ggcgttaata agccgctaaa acgtagctcg tcgagaccat catctgccag ccgcgttctg
    17881 gtgtaatcgg aactgcgcac ggtaagttct tcggtatcgg cgcgcgcatc gatccacggc
    17941 tggcgtagtt ttgccagccc ttgctgcacg ttaatggcga tctgcggatc accatacggg
    18001 ccggaggtgt cgtagaccgg aatcgcttcg ttttcttcgt actgcggctg ttctttgcta
    18061 ccgccaatta gcgtcgggct aagctggatc tcacgcatcg gcacgcgcac gccggggtgt
    18121 gtgccagtga tataaatgcg ttttgagttg ggaaaggcgg tgccttccag ggtgtcgata
    18181 aaatgttggg cccgggcgcg ttgttcgcgg cgggtcagtt ttgttgcaga catagctcat
    18241 tccaaaaagt taaggacgtg gcttgtcaga cgacggatga agcaagagac gatcgccgca
    18301 aaggcgatgc gatagcagat taactcttgt tcccttcgca ggtattagcc tgatcaggtt
    18361 ccgcggatcc cgaataaacg gtctcagcca gttaaggcac tccgacaaga aattagcccc
    18421 cacaaacggg gcgttgaatg taaattacgc gttaacagcg cagaactcaa gcaggatgtt
    18481 tgacgcgggc ggcgtcaagc accagcagaa tcagcttatc ttccagcaca aagcgcgctt
    18541 ccagcgcctc gccgatgtca gataaaacct gttgaaactc aaggtaatta tcatgatcga
    18601 tggcggtttc caggctggaa tcgtagtaat ccataatctg ttgggtattg gcttcgagtt
    18661 gcggccaaat cttcgcggcg cgcgcgagtt gcccgttgcc ttccagctta tgaagaatgc
    18721 gctcataaat actgaaatgt ccggcagaca agtaatcgac caggctctga caaaaatcat
    18781 caagggcttt ttcgtttagc ctcatgtacg attctttgcc aggcttaatg ccaaccagat
    18841 tgtagtaagc cacgagcaga tgcttacgta catgtagcca gcgatcaacc agtttgttac
    18901 ttcctctgac gcgctccgtc aggttatcga gctggttaag catgattgac tccgcaagtt
    18961 tgtattcaaa aactgctcag tgagaaatgt aaaaaccatg ttaaacatgc cagtgatgca
    19021 aaggtagtgc aagagctatg gatcgtataa ttgaaaaatt agatcacggc tggtgggtcg
    19081 tcagccatga acaaaaatta tggttgccga agggagaatt gccatatggc gaagcggcaa
    19141 atttcgatct tgtgggtcag cgcgcactac aaatcggcga atggcagggg gaacctgttt
    19201 ggttaataca acagcagcgg cgtcacgata tggggtcggt acgtcaggtc attgatctcg
    19261 atgttgggct gtttcaactg gccggacgtg gcgtgcaact ggcggagttt taccgatcgc
    19321 ataaatactg tggttactgc gggcatgaaa tgtatccgag caaaaccgaa tgggcgatgc
    19381 tgtgtagcca ttgccgtgag cgttactacc cgcaaatcgc cccctgcatt attgttgcca
    19441 tccgtcgcga tgattcgctc ctcctcgccc agcatacccg ccatcgtaac ggtgtccata
    19501 cagtacttgc cggattcgtc gaagtgggcg aaaccctcga gcaggcagtc gcgcgggaag
    19561 tgatggaaga gagcggaatt aaagttaaaa acttgcgtta cgtgacttct cagccgtggc
    19621 cgtttcctca gtctttaatg accgcgttta tggcggaata tgacagcggc gacatcgtga
    19681 tcgacccgaa agaattgctc gaggcgaact ggtatcgcta tgacgatttg ccgttactcc
    19741 cgccgcccgg caccgtagcg cgccgtctga tagaagatac tgtggcgatg tgtcgggcag
    19801 agtatgagtg atgatacact gaccgcctga cgcactaagg aacagccaaa atgaccgaac
    19861 ttaaaaacga tcgttatctg cgggcgctgc tgcgccagcc cgttgatgtc actccagtat
    19921 ggatgatgcg ccaggcgggt cgctatctac cggaatataa agccacgcgc gcccaggcgg
    19981 gcgattttat gtcgctgtgc aaaaacgccg agctggcgtg cgaagtgact ttgcaaccgc
    20041 tgcgtcgcta cccgctggat gcggcgatcc tcttttccga tatcctcacc gtgccggacg
    20101 cgatggggtt agggctctat tttgaagccg gagaaggtcc gcgttttacc tcgccagtca
    20161 cctgcaaagc cgacgtcgat aaactgccaa ttccggaccc ggaagatgag ctgggttacg
    20221 tgatgaacgc ggtgcgtacc attcgtcacg aactgaaagg cgaagtgccg ctgattggtt
    20281 tttccggcag cccgtggacg ctggcaacct acatggtgga aggcggcagc agcaaagcct
    20341 tcaccgtgat caaaaaaatg atgtatgccg atccgcaggc gctgcacgct ctgctcgata
    20401 aactggcgaa aagcgtcact ttgtatctga atgcgcagat taaagccggt gctcaggcag
    20461 tgatgatttt cgacacctgg ggcggcgtgc ttaccgggcg cgattatcaa cagttctcgc
    20521 tctattacat gcataaaatt gttgatggtt tactgcgtga aaacgacggt cgccgcgtgc
    20581 cagtcacgct gtttaccaaa ggcggcggac agtggctgga agccatggct gaaaccggtt
    20641 gcgatgcgct gggcctcgac tggacaacgg acatcgccga tgcgcgccgc cgtgtgggca
    20701 ataaagtcgc gttgcagggt aatatggatc cgtcgatgct gtacgcgccg cctgcccgca
    20761 ttgaagaaga agtagcgact atacttgcag gtttcggtca cggcgaaggt catgtcttta
    20821 accttggtca cggcattcat caggatgtgc cgccagaaca tgctggcgtg ttcgtggagg
    20881 cagtgcatcg actgtctgaa cagtatcacc gctaagagcc tgatccatca ggctatttta
    20941 tttgccattt tgaggccggg cagtgctcaa aatcctcacg tactacgtgt acgctccggt
    21001 ttttccgcgc tgtccggcct caaactgcct tcaacaatta cgcctgttga accaggctct
    21061 aaggagcgat tatggatctc gcgtcattac gcgctcaaca aatcgaactg gcttcttctg
    21121 tgatctgcga ggatcgactc gataaagatc caccggatct gatcgccgga gccgatgtcg
    21181 ggtttgagca gggcggagaa gtgacgcgag cggcgatggt gctgctgaaa tatccctcgc
    21241 ttgagctggt ggagtataaa gttgcccgca tcgccaccac catgccttac attccaggtt
    21301 ttctttcctt ccgcgaatat cctgcgctgc tggcagcgtg ggagatgctg tcgcaaaagc
    21361 cggatttagt gtttgtcgat ggtcatggga tctcgcatcc tcgccgtctt ggcgtcgcca
    21421 gccattttgg cttattggtg gatgtgccga ccattggcgt ggcgaaaaaa cggctctgcg
    21481 gtaaattcga accgctctcc agcaaaccgg gcgcgctggc cccactgatg gataaaggcg
    21541 agcagctggc ctgggtctgg cgcagcaaag cgcgctgtaa cccgttgttt atcgctaccg
    21601 gccatcgggt cagcgtggac agcgcgctgg cgtgggtaca acgctgcatg aaaggctatc
    21661 gtctgccgga gccaacgcgc tgggcggacg cggtggcctc ggaacgtccg gcgttcgtgc
    21721 gctatacagc aaatcagccc taattcaggt aaactgcgga taatttccgt atttgagaac
    21781 tcaacatgtt acaaaaccca attcatctgc gtctggagcg cctggaaagc tggcagcatg
    21841 tcactttcat ggcttgctta tgcgaacgca tgtaccccaa ttacgccatg ttctgccagc
    21901 aaaccggttt tggtgatggg caaatttacc gtcgtattct cgatctcatc tgggaaacgc
    21961 tgaccgttaa agacgcaaaa gtaaatttcg acagccaact ggagaaattt gaagaagcga
    22021 ttccttcagc cgacgatttc gatctgtacg gcgtttatcc ggcaatcgat gcctgcgtgg
    22081 cgttaagtga actggtccat tcgcgtttga gtggtgaaac gctcgaacac gcggtggaag
    22141 tgagtaagac ctccatcacg accgttgcga tgctggaaat gactcaggct ggtcgcgaaa
    22201 tgggcgatga agagcttaaa gaaaacccag ctgtagagca agaatgggac attcagtggg
    22261 aaatattccg acttttagct gagtgcgaag aacgcgatat cgagctgata aaaggcctta
    22321 gggcagacct gcgtgagtcg ggtgagagca atattggtat aatttttcag caataagacc
    22381 agaaaacgtg atttaacgcc tgatttgtcg tacctggagt cttccctttc gccccccgtc
    22441 tggtctacat ttggggggcg aaaaaaagtg gctatcggtg cgtgtatgca ggagagtgct
    22501 attctggcat ttccgtcgca ctcgatgctt agcaagcgat aaacacattg taaggataac
    22561 ttatgaacaa gactcaactg attgatgtaa ttgcagagaa agcagaactg tccaaaaccc
    22621 aggctaaagc tgctctggag tccactctgg ctgcaattac tgagtctctg aaagaaggcg
    22681 atgctgtaca actggttggt ttcggtacct tcaaagtgaa ccatcgcgct gagcgtactg
    22741 gccgcaaccc gcagaccggt aaagaaatca aaattgccgc agctaacgta ccggcatttg
    22801 tttctggcaa ggcactgaaa gacgcagtta agtaagattg cgtggcagtg aacagtttta
    22861 acgaaggggt ggtttcaccc cttttgtctt tctggcgtcg atcattgatg ctggcaggtg
    22921 cgctgtttct cactgcctgt agccacaact cttcacttcc tccctttacc gccagtggat
    22981 ttgctgaaga ccagggcgcg gtacgcatct ggcgaaaaga cagcggcgat aatgtgcatc
    23041 tgcttgccgt gtttagcccg tggcgcagtg gcgataccac gacgcgagag tatcgctggc
    23101 agggcgataa cctcacgctc atcaatatca atgtttacag caaaccgccg gtgaacattc
    23161 gcgcgcgttt tgacgatcgc ggcgatctga gctttatgca acgtgaatcc gatggggaaa
    23221 agcagcagct ttctaacgac caaatcgatt tataccgtta tcgtgctgat cagatccgcc
    23281 agattagcga tgccttacgt caggggagag tcgtgctgcg ccaggggcgc tggcatgcga
    23341 tggaacagac cgtgaccacc tgcgaagggc aaaccattaa acctgattta gattcgcagg
    23401 cgatagcgca tatcgagcgc cgccagagcc gctcttctgt tgatgtcagc gtggcatggc
    23461 tggaagcgcc cgaaggttcg caattactgt tagtggcaaa ctctgatttc tgtcgctggc
    23521 aacccaacga gaaaacgttc tgatttacca gtggcccata cccatatgac cgccaccacc
    23581 gcagccgccg tagcccatgc ccattccggc tccgcgcgga atacccgctt cagccatcgc
    23641 gatatctcgt ttcacccgta actcatctaa cgactgacgc aaattctcca tctctttggc
    23701 gaccgcgtta attttgctgc tatccggtgg gttcgcggct aacagggcat tgtattcata
    23761 acgcttcgtc accagttgct gttgcagtgc gctgctttga gcgtaaaagt cattatggat
    23821 tttctgccac gctgtctgtt gttcgctggt caaaggcgcg gcattttgct gccacatacc
    23881 gtgtccgccg tgagcaaatg cagatgtcga tcccatcgcc attgctgaaa gcgccatcat
    23941 taccagggca attttcgtgt tccgtttcat ggttaatcct ccagtggttg tcttacttcg
    24001 ggtattgcat cttccgtgcc aacgatgaaa cgctgatatg acgggtaatc tggcatgata
    24061 aacgagtaaa aatgactcgc ctgctgcggg tagcgagtca tttttactca ttgaaactta
    24121 agcctttgtg ttacagcgca gggtaagcgc tgataaaaga tggcatgatt tctgctgtca
    24181 gaaagggatg agcaggcaaa gaagaagatg cgttttatgc aacgttctaa agactcctta
    24241 gctaaatggt taagcgcgat cctccccgtg gtcattgttg ggctggtagg gttgtttgcg
    24301 gtgacggtga ttcgcgatta tgggcgcgag actgccgccg ccagacaaac gctgctggaa
    24361 aaaggcagtg tacttattcg cgctcttgaa tccggctcgc gcgtcggcat ggggatgcgc
    24421 atgcatcatg cgcagcagca ggccttactg gaagaaatgg ccgggcagcc tggagtacgt
    24481 tggtttgcgg tcacggatga acaaggaaca atcgtgatgc atagcaactc cggcatggtg
    24541 ggaaaacagc tttattcccc tcaggaaatg cagcagttac atccgggaga tgaagaagcg
    24601 tggcggcgga tcgatagcgc agacggcgag cctgttctgg aaatttatcg ccagtttcaa
    24661 ccgatgtttg ctgctggaat gtaccggatg cgccatatgc agcagtatgc cgcgacacca
    24721 caagcaattt tcatcgcttt cgacgccagt aatattgtga gtgccgaaga tcgtgagcag
    24781 agaaacaccc tgattattct cttcgccctg gcgacggtct tgctggcaag cgtattgtca
    24841 ttcttctggt atcgccgcta tctgcgctcg cgccagcttc tacaagatga aatgaagcgc
    24901 aaagagaagc tggtggcgct ggggcatctt gcggcaggcg ttgcccacga aatccgtaac
    24961 ccactttcct cgattaaagg actggcgaaa tactttgccg agcgcgcgcc tgcaggggga
    25021 gaagcgcatc aactggcgca ggtgatggcg aaagaggccg accgtttaaa ccgcgtggta
    25081 agcgagttgc tggaactggt taagccaacg catctggctt tgcaggcggt ggatctcaac
    25141 acgctgatta accactcatt acagctggta agccaggatg caaacagccg ggagatccag
    25201 ttacgcttta ccgccaacga cacattaccg gaaattcagg ccgatccgga caggctgact
    25261 caggtcctgt tgaatctcta tctcaatgct attcaggcga ttggtcagca tggcgtgatt
    25321 agcgtgacgg ccagcgaaag cggcgcgggc gtgaaaatca gcgttaccga cagcggtaag
    25381 ggaattgcgg cagatcagct tgaagccatc ttcactccgt acttcaccac caaagccgaa
    25441 ggcaccggat tggggctggc ggtcgtgcat aatattgttg aacaacacgg tggtacaatt
    25501 caggtcgcaa gccaggaggg aaaaggctca acgttcaccc tctggcttcc ggtcaatatt
    25561 acgcgtaagg acccacaagg atgacgcacg ataatatcga tattctggtg gtggatgatg
    25621 acattagcca ctgcactatt ttgcaggctt tactgcgcgg ctggggctat aacgtcgcgc
    25681 tggcgaacag cgggcgacag gcgctggagc aggtgcggga acaggttttt gatcttgtgc
    25741 tttgcgatgt gcgaatggcg gagatggacg gcatcgccac gctgaaagag atcaaagcgt
    25801 taaacccggc aattccggtg ctgattatga ctgcgtactc cagcgtcgag acggcggtag
    25861 aggcactgaa aactggggcg caggattatc tcatcaagcc gctggatttc gataacctac
    25921 aggcgacgct ggaaaaggcg ctcgcgcata cgcacagtat tgatgctgaa acacctgcgg
    25981 tgactgccag ccagttcggt atggtcggta aaagcccggc gatgcaacac ctgctcagtg
    26041 aaatcgccct cgtcgcgcca tcggaagcca cggtgctgat ccacggcgat tccggcaccg
    26101 gtaaagagct ggtcgccagg gcgattcacg ccagtagcgc acgtagcgaa aaaccgctgg
    26161 taacgctcaa ctgtgcggca ctcaacgaat ccttgctgga atctgaattg ttcggtcacg
    26221 aaaaaggggc gtttactgga gccgataaac gccgggaagg gcgctttgtt gaggcggacg
    26281 gcggcacgct gtttctcgat gaaattggcg atatctcgcc gatgatgcag gtgcgtctgc
    26341 tgcgtgcgat tcaggagcgc gaagttcagc gtgtcggtag caaccagact atctcggttg
    26401 atgtccggct gattgcggcg acccatcgcg atcttgccgc agaggtgaat gccgggcgtt
    26461 ttcgccagga tctctactat cgcctgaatg tggtggcgat tgaagtacca tcgctgcggc
    26521 aacggcggga agatatccct ctgctggctg gccattttct tcagcgcttt gccgagcgta
    26581 atcgcaaggc ggtaaaaggc tttacgccac aggcgatgga tttgctgatc cactacgact
    26641 ggccgggaaa tattcgtgaa ctggaaaacg cggtggaacg ggcggtggtg ctgctgaccg
    26701 gggaatatat ttccgaacgc gagctgccgc tggcgattgc cagtacgccg atcccgctgg
    26761 ggcaaagtca ggatattcag ccgctggtgg aagtggaaaa agaggtgatt ctggcggcgc
    26821 tggagaaaac gggcggcaac aaaaccgaag ccgcccgtca gttagggatc acgcgcaaaa
    26881 cgctattggc aaaactgtcg cgttagttct gctcgcgttc gatggcgcgc caaccgatat
    26941 ctttccggca gaagcaatca tcccagtgga tatcggtcat taaggcgtag gcgcgtttct
    27001 gcgcttctgc cacggtatga cccagcgcgg tgacgcacag tacgcgcccg ccgctggtta
    27061 ccacctgctc gtcatccgcc agttttgtgc ccgcgtggaa cactttgccg tcagccactt
    27121 cttccagcgg caggccgtgg atcacatcgc cggtgcggta atcgcccgga tatccacccg
    27181 cagccatcac cacgctgaga gaagcacgtt catcccactc tgacgttttc tcgtccagtt
    27241 tgccttcaca ggccgccagg cagagctcaa ccagatcgga cttcatgcgc agcataatcg
    27301 gctgggtttc cggatcgcca aagcggcagt taaattcaat aaccttcgga ttgccctgtt
    27361 tgtcgatcat caggcccgca tagagaaaac cggtgtaggt gttgccttcc gccgccatgc
    27421 ctttcacggt tggccagatg atacgttcca tggtgcgctg atgaacttca tcggttacta
    27481 ccggcgcggg ggagtaagcg cccatcccgc cggtgtttgg tccgttatct ttatcgccta
    27541 cgcgtttgtg atcctggctg gtagccatcg gcagcacatg ctcgccgtcc accatcacga
    27601 taaagctcgc ttcttcgcca tcgaggaact cttcgataac gatgcgatga cccgcgtcgc
    27661 caaaagcgtt gcccgccagc atatcgtgaa cagccgcttc cgcttcttcc agcgtcatcg
    27721 ccacgataac gcctttcccg gcagccagac cgtccgcttt aatgacgatt ggcgcgcctt
    27781 tctcacgcag atacaccagc gcaggttcta cctcggtgaa gttctggtat tccgccgtag
    27841 ggatcttatg gcgggccagg aaatctttgg taaacgcttt tgagccttcc agttgggccg
    27901 cacctgcggt tgggccgaag attttcagcc cggcggcgcg gaaggtatcg accacgcctt
    27961 tcaccagcgg cgcttccggg ccgacgatgg tcagatcaat cttttcgttt tgtgcgaaat
    28021 ccaacagcgc cgggatatcg gtcacgccga tagcaacgtt ttgcagcgcg ggttccagcg
    28081 cagtgcctgc attacccgga gcagcaaaaa cagtctcaac cagcggcgac tgggccgctt
    28141 tccaggccag cgcgtgctcg cgcccgccgt taccaatcac taatactttc atctattgct
    28201 ccattaatgg cggaagtggc gcatgtcggt gaagagcatc gcaataccgt gctcgtcggc
    28261 ggcggcaatc acttcgtcat cacggatgga accgccaggc tggattacgc aggtcacgcc
    28321 cgcagcggcg gcggcatcaa taccgtcgcg gaacgggaag aacgcgtcag aagccatcga
    28381 ggaacctttc acttccaggc cttcatcggc agctttaata ccggcgattt tcgcggagta
    28441 cacgcggctc atctggcccg cgccaatacc gatggtcata ttgtttttgg catagacgat
    28501 agcattggat ttcacgaatt tcgccacttt ccagcagaac agcgcatcac gcagttcctg
    28561 ttcggtcggc tgacgttggg tgacgacgcg cagttcttcc gcaccgacca tccccaggtc
    28621 acgatcctga accagcagac cgccgttcac gcgtttgaaa tcaagacccg gaacacgctc
    28681 gccccactga ccgcaggtca gaacgcgtac gttctgcttg gcggcggtga ttttcagggc
    28741 ttcttcgctg gcggaaggcg caataatcac ttcgacaaac tgacgagaaa tgatggcctg
    28801 tgcggtttcc gcatccagct cgcggttaaa ggcaatgatg ccgccgaatg cggaggttgg
    28861 gtcggttttg tacgcgcgat cgtaagcatc aagaatggaa ttgccgatag ctacaccgca
    28921 agggttggcg tgcttcacaa tcacacatgc cggctcggcg aactctttca cgcactccag
    28981 cgccgcatcg gtatcggcga tgttgttata agagagggct ttaccctgaa cctgggttgc
    29041 ggtagcaacg gaggcttctt tcacattctc ttctatatag aaggcagcct gctggtggct
    29101 gttctcgccg taacgcatat cctgcttctt aatgaagttc aggttcagcg tgcgtgggaa
    29161 gcgaccggcg gcttctttgc tttcaccgtg gtacgccgga accatgctgc cgaagtagtt
    29221 ggcaatcatg ctgtcgtagg cggcagtgtg ttcgaaggct ttgatggcga ggtcgaaacg
    29281 ggttgcaagc gtcagcgatc cttcgttgtc atccatctct ttaataatgg cgtcgtagtc
    29341 gctgctcttc accacgattg cgacatcttt atggttctta gcggcggagc gcaccatggt
    29401 cgggccgccg atatcgatgt tctcaaccgc atcttccagc gagcagcctt cacgagcaac
    29461 agtctgggcg aacggataca ggttaacaac caccatatcg ataggctgga tctgatgttc
    29521 ttccataatg gcatcgtcct ggccgcgacg gcccagaatg ccaccatgta ctttcggatg
    29581 cagggtcttc acgcgtccat ccatcatctc cgggaaaccg gtgtaatcgg aaacttcggt
    29641 taccggcaga cctttttctg ctaacagacg ggcagtgccc cctgtagaca gcagctccac
    29701 accgcgtgcg gaaagtgcct gggcgaattc gacgataccg gctttgtcag aaacactgag
    29761 cagagcgcgg cggactggac gacgttgttg catggtaaat cccctgggtt tgactattac
    29821 agagagcgtt agctgaattt ttcgcgaaaa actcagctaa cgcccctaac ggggcatcct
    29881 tatttttcgc ccgcattgta acgaaaacgt ttgcgcaacg ctcgcgaatt tttctctttc
    29941 aatggtgatc acaattttga cagtggttac cgtgggaaaa atacagaaat tacattgatg
    30001 attgtggata actctgtgcg taaaaaggta taaagcgggg ttttgctggg gaatgcagca
    30061 gtcagtcatt tttctgcaat ttttctattg cggcctgcgg agaactccct ataatgcgcc
    30121 ttcatcgaca cggcggatgt gaatcacttc acacaaacag ccggttcggt tgaaga
//
LOCUS       DAZSIO010000031        30131 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZSIO010000031 DAZSIO010000000
VERSION     DAZSIO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30131
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.031"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="ACHX5T_001284"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="ACHX5T_001284"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR8466553.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="ACHX5T_001285"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="ACHX5T_001285"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR8466554.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="ACHX5T_001286"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="ACHX5T_001286"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR8466555.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="ACHX5T_001287"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="ACHX5T_001287"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR8466556.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="ACHX5T_001288"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="ACHX5T_001288"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR8466557.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="ACHX5T_001289"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="ACHX5T_001289"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR8466558.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="ACHX5T_001290"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="ACHX5T_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR8466559.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="ACHX5T_001291"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="ACHX5T_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR8466560.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="ACHX5T_001292"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="ACHX5T_001292"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR8466561.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="ACHX5T_001293"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="ACHX5T_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR8466562.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="ACHX5T_001294"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="ACHX5T_001294"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR8466563.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="ACHX5T_001295"
     CDS             complement(15665..16702)
                     /locus_tag="ACHX5T_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR8466564.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="ACHX5T_001296"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="ACHX5T_001296"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR8466565.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="ACHX5T_001297"
     CDS             complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="ACHX5T_001297"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR8466566.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19444..19884
                     /locus_tag="ACHX5T_001298"
     CDS             19444..19884
                     /locus_tag="ACHX5T_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR8466567.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNSDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="ACHX5T_001299"
     CDS             complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="ACHX5T_001299"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR8466568.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20611..21544)
                     /locus_tag="ACHX5T_001300"
                     /pseudo
     CDS             complement(20611..21544)
                     /locus_tag="ACHX5T_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="ACHX5T_001301"
     CDS             complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="ACHX5T_001301"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR8466569.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="ACHX5T_001302"
     CDS             complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="ACHX5T_001302"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR8466570.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="ACHX5T_001303"
     CDS             complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="ACHX5T_001303"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFR8466571.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25143..25856)
                     /gene="livF"
                     /locus_tag="ACHX5T_001304"
     CDS             complement(25143..25856)
                     /gene="livF"
                     /locus_tag="ACHX5T_001304"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR8466572.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25858..26625)
                     /gene="livG"
                     /locus_tag="ACHX5T_001305"
     CDS             complement(25858..26625)
                     /gene="livG"
                     /locus_tag="ACHX5T_001305"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR8466573.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26622..27899)
                     /gene="livM"
                     /locus_tag="ACHX5T_001306"
     CDS             complement(26622..27899)
                     /gene="livM"
                     /locus_tag="ACHX5T_001306"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR8466574.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27896..28822)
                     /gene="livH"
                     /locus_tag="ACHX5T_001307"
     CDS             complement(27896..28822)
                     /gene="livH"
                     /locus_tag="ACHX5T_001307"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR8466575.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28870..29979)
                     /gene="livK"
                     /locus_tag="ACHX5T_001308"
     CDS             complement(28870..29979)
                     /gene="livK"
                     /locus_tag="ACHX5T_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR8466576.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
BASE COUNT         7271 a         8411 c         7608 g         6841 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcaca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctctaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagctgcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt caacgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctacttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattgc cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgatacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cacccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgtcgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc
    19261 taaaacaact tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta
    19321 tcatcgttat tctccagaga ttaaggggca accccagcta agcctggtat ataactctga
    19381 attaatcatc gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac
    19441 gcgatgaaac gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc
    19501 attaatactc tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg
    19561 caaacccaga tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa
    19621 acgcaaactc agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg
    19681 gtgttgcagt cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc
    19741 aacggcggga tgttaaacag caaccgtaat ccggatagtt cgttgaatca gcagcatatg
    19801 ctgccggaga ggagaaacag cgacatgctg aatcagccca gcacgccgca gcctgacatt
    19861 ccgttgaaaa ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga
    19921 tgcaatccac gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa
    19981 ccagaatccg taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat
    20041 gattgagatg aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc
    20101 actcatcaag caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag
    20161 catcaatctc aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg
    20221 ccagcgccac cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat
    20281 tcgccatcat cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct
    20341 cacctttaaa cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt
    20401 tcagttcacc cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca
    20461 ggaagatctc gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt
    20521 ttgccccgac gtcgattgcc gccagggtgt tttccggggc cagcttaccg ccgccacgat
    20581 gagcgacgat gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa
    20641 aagatgcagc tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc
    20701 ctgatgcgcc agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc
    20761 gaatgccgag ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca
    20821 cgataccgcc gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt
    20881 tcatcgccgg gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt
    20941 ccaccggcgt gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga
    21001 gcgtcatcgc ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt
    21061 gcagctgttg cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg
    21121 gctcatcaaa caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct
    21181 ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa
    21241 tgcgcgccgc ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca
    21301 gcccccacgc catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca
    21361 ccatcgcaat cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga
    21421 tatcgccttc ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc
    21481 agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt
    21541 taatgaccta ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca
    21601 tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc
    21661 actactggtg ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg
    21721 ccttcgccag tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg
    21781 gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac
    21841 agcgccgcca gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt
    21901 gacgcaccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg
    21961 cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg
    22021 tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc
    22081 agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag
    22141 acaatggcaa atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc
    22201 atcacaaagc tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc
    22261 acccagatgt tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg
    22321 gcgtagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg
    22381 atcaccgcga tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc
    22441 tcaatcattg gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga
    22501 tgacgaggaa catcagcacc atcgactgtg cggcagacga agccagatcc agtccggtaa
    22561 aaccttcgcg gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg
    22621 tggcggcgtc gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca
    22681 ggaagaaact caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc
    22741 ctgcaccgtc gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat
    22801 agaagaacag aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca
    22861 tcgcctgacc gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg
    22921 tgatcagccc gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag
    22981 ccacggcata cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact
    23041 ccaccagcgc cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga
    23101 attttatcgt cgtccagaag gagtcgagat aatagctgtc atgaaacagc gtgacgaagt
    23161 tatccagacc gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc
    23221 acaacgcttc gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga
    23281 ccagcagata aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta
    23341 attcctgaac tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga
    23401 taagcgaaac gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc
    23461 agtaactggt tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg
    23521 gtccacacgc tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga
    23581 cgaagccctt tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc
    23641 gcacctgggt ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta
    23701 attggcagat aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag
    23761 tcgagaaact tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg
    23821 ctggctccgc cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc
    23881 atacctacgc cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag
    23941 gcggtggtca tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg
    24001 acgtagctga agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc
    24061 tccggcttgt tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca
    24121 aacggcagac cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg
    24181 gcgtagccgc acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc
    24241 caggttttcg gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa
    24301 tagagaacgg aagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg
    24361 gagtagtaac cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct
    24421 ttaaacacgt catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact
    24481 tcataaacct gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt
    24541 aaattctgtt cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg
    24601 ttaaaacgtt gggccagaga atccacctct ttacccagtt ccccttccat agaatgccag
    24661 aacggaatgg tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct
    24721 gaagctgtat aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat
    24781 tcacgcgttt tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt
    24841 ttgttacatt tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt
    24901 gagggaaaat gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc
    24961 tacagattac tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga
    25021 tcgtgcaaat tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg
    25081 caattttgca tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt
    25141 tattacccgc ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta
    25201 tcggagagca ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc
    25261 tggttggcgt tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg
    25321 gtgtcgaaaa tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac
    25381 aacaaacgcg gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg
    25441 gacatggtgc ccgcccgctg aacacggcgc tcatgcagac gtggaaacag ttcatacacc
    25501 cactttatgc gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc
    25561 tcttccaccg tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg
    25621 cgcatgattt tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg
    25681 ctggtggcgc gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc
    25741 gcgccaatga gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc
    25801 tggattttgc cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta
    25861 tgcctcacct aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc
    25921 gtttgccagt ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag
    25981 cttcatatcg tgttcaatca acaaaatagt ggtgttgtga tgattgcgca gttcggcaat
    26041 cagctcatcc agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat
    26101 taaaatctcc ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata
    26161 ggccaggtta ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt
    26221 ggcggcgcgg tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc
    26281 agagaacagc ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt
    26341 catttcacgg aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg
    26401 ctgccccggt aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt
    26461 gtagaaaccg gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa
    26521 cgagacgatt tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc
    26581 gaagcgcatc atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc
    26641 gctgcgccgt ttttcagctt cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc
    26701 cagatcatca tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca
    26761 cgcatcaatt cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag
    26821 cccataccgc cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat
    26881 tccgggctga caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg
    26941 gcgctgatgg taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag
    27001 gcgatctcgt cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg
    27061 ttaatgacaa acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg
    27121 cgatcggacg gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca
    27181 cgagcggtac ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg
    27241 ttcgggccgc cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa
    27301 ccaagggtaa cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg
    27361 aaacccgccg ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg
    27421 taatagtgat tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc
    27481 agcaccagca gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag
    27541 atcatggtca gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc
    27601 acaagcacca gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg
    27661 ggcagaataa acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa
    27721 agctggaaga aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa
    27781 gccgtgtcga ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc
    27841 gccaggacaa agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata
    27901 ctttctctac ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca
    27961 gcagggcgaa tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct
    28021 ccgcaatccc caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga
    28081 gcaccgcagc ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc
    28141 agtagaactg accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg
    28201 tcaaagcaat cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg
    28261 cgcaggcacg acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgccagca
    28321 tggcgaggaa ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt
    28381 tttcgctatg gcccaccacc cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg
    28441 aaccttcggt caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa
    28501 gcgcaatcag gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc
    28561 cgtaggcgct ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg
    28621 tatcaatgcc catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca
    28681 tataaacctc gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc
    28741 cgatggctat cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga
    28801 aatacaaaaa ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg
    28861 gtgggatgat cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa
    28921 atcaaatccc ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc
    28981 accgttagct tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag
    29041 ggcagttgcc agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc
    29101 tttcttgtct gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg
    29161 ttttggcata gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc
    29221 gttaccgaca ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg
    29281 cagcatctgc cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt
    29341 tttcaggcgg gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa
    29401 gacgacgttg gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc
    29461 atactgttgt ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta
    29521 ttttgccgcc gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata
    29581 accgcgttgg gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc
    29641 ttcgtagata tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt
    29701 aatgccgtcg ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc
    29761 gtcatattcc acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt
    29821 aatcgcctga cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat
    29881 cgcgccgaca acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc
    29941 aatcatccct gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg
    30001 atgttgtgtt gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca
    30061 ctgtttttgc ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa
    30121 tccctatttt t
//
LOCUS       DAZSIO010000032        29788 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZSIO010000032 DAZSIO010000000
VERSION     DAZSIO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.032"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..851
                     /locus_tag="ACHX5T_001309"
     CDS             <1..851
                     /locus_tag="ACHX5T_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR8466577.1"
                     /translation="RHIIIGNEGALCYITEVTVKIFKYTPENNLFYGYILEDMKTGFN
                     ILREIMVEGYRPSIARLYDAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEI
                     VARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEI
                     YESVINRIRTEFPHADDITMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNP
                     LNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGT
                     IYPIEK"
     gene            873..2282
                     /locus_tag="ACHX5T_001310"
     CDS             873..2282
                     /locus_tag="ACHX5T_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8466578.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2260..3039
                     /locus_tag="ACHX5T_001311"
     CDS             2260..3039
                     /locus_tag="ACHX5T_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR8466579.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3036..3896
                     /locus_tag="ACHX5T_001312"
     CDS             3036..3896
                     /locus_tag="ACHX5T_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR8466580.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4044..4619)
                     /locus_tag="ACHX5T_001313"
                     /pseudo
     CDS             complement(4044..4619)
                     /locus_tag="ACHX5T_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4636..4896)
                     /locus_tag="ACHX5T_001314"
     CDS             complement(4636..4896)
                     /locus_tag="ACHX5T_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR8466581.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4887..6158)
                     /locus_tag="ACHX5T_001315"
     CDS             complement(4887..6158)
                     /locus_tag="ACHX5T_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR8466582.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6236..6598)
                     /gene="queD"
                     /locus_tag="ACHX5T_001316"
     CDS             complement(6236..6598)
                     /gene="queD"
                     /locus_tag="ACHX5T_001316"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR8466583.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6917..8716
                     /gene="cysJ"
                     /locus_tag="ACHX5T_001317"
     CDS             6917..8716
                     /gene="cysJ"
                     /locus_tag="ACHX5T_001317"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR8466584.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8716..10428
                     /gene="cysI"
                     /locus_tag="ACHX5T_001318"
     CDS             8716..10428
                     /gene="cysI"
                     /locus_tag="ACHX5T_001318"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR8466585.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10502..11236
                     /gene="cysH"
                     /locus_tag="ACHX5T_001319"
     CDS             10502..11236
                     /gene="cysH"
                     /locus_tag="ACHX5T_001319"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR8466586.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11500..11652
                     /locus_tag="ACHX5T_001320"
     CDS             11500..11652
                     /locus_tag="ACHX5T_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR8466587.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11846..12926
                     /locus_tag="ACHX5T_001321"
                     /pseudo
     CDS             11846..12926
                     /locus_tag="ACHX5T_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13072..14109)
                     /gene="iap"
                     /locus_tag="ACHX5T_001322"
     CDS             complement(13072..14109)
                     /gene="iap"
                     /locus_tag="ACHX5T_001322"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR8466588.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14361..15269
                     /gene="cysD"
                     /locus_tag="ACHX5T_001323"
     CDS             14361..15269
                     /gene="cysD"
                     /locus_tag="ACHX5T_001323"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR8466589.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15271..16698
                     /gene="cysN"
                     /locus_tag="ACHX5T_001324"
     CDS             15271..16698
                     /gene="cysN"
                     /locus_tag="ACHX5T_001324"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR8466590.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16698..17303
                     /gene="cysC"
                     /locus_tag="ACHX5T_001325"
     CDS             16698..17303
                     /gene="cysC"
                     /locus_tag="ACHX5T_001325"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR8466591.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17353..17676
                     /locus_tag="ACHX5T_001326"
     CDS             17353..17676
                     /locus_tag="ACHX5T_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR8466592.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17870..18181
                     /gene="ftsB"
                     /locus_tag="ACHX5T_001327"
     CDS             17870..18181
                     /gene="ftsB"
                     /locus_tag="ACHX5T_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR8466593.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18200..18910
                     /gene="ispD"
                     /locus_tag="ACHX5T_001328"
     CDS             18200..18910
                     /gene="ispD"
                     /locus_tag="ACHX5T_001328"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR8466594.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18910..19389
                     /gene="ispF"
                     /locus_tag="ACHX5T_001329"
     CDS             18910..19389
                     /gene="ispF"
                     /locus_tag="ACHX5T_001329"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR8466595.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19386..20435
                     /gene="truD"
                     /locus_tag="ACHX5T_001330"
     CDS             19386..20435
                     /gene="truD"
                     /locus_tag="ACHX5T_001330"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR8466596.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20416..21177
                     /gene="surE"
                     /locus_tag="ACHX5T_001331"
     CDS             20416..21177
                     /gene="surE"
                     /locus_tag="ACHX5T_001331"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR8466597.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21171..21797
                     /gene="pcm"
                     /locus_tag="ACHX5T_001332"
     CDS             21171..21797
                     /gene="pcm"
                     /locus_tag="ACHX5T_001332"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR8466598.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21982..23076
                     /gene="nlpD"
                     /locus_tag="ACHX5T_001333"
     CDS             21982..23076
                     /gene="nlpD"
                     /locus_tag="ACHX5T_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR8466599.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23139..24131
                     /gene="rpoS"
                     /locus_tag="ACHX5T_001334"
     CDS             23139..24131
                     /gene="rpoS"
                     /locus_tag="ACHX5T_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR8466600.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24251..24658)
                     /locus_tag="ACHX5T_001335"
     CDS             complement(24251..24658)
                     /locus_tag="ACHX5T_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFR8466601.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24805..25398
                     /locus_tag="ACHX5T_001336"
     CDS             24805..25398
                     /locus_tag="ACHX5T_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFR8466602.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25398..26345
                     /locus_tag="ACHX5T_001337"
                     /pseudo
     CDS             25398..26345
                     /locus_tag="ACHX5T_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26618..27181)
                     /locus_tag="ACHX5T_001338"
     CDS             complement(26618..27181)
                     /locus_tag="ACHX5T_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466603.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27346..27597
                     /locus_tag="ACHX5T_001339"
     CDS             27346..27597
                     /locus_tag="ACHX5T_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8466604.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27599..29695
                     /locus_tag="ACHX5T_001340"
     CDS             27599..29695
                     /locus_tag="ACHX5T_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFR8466605.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7289 a         7381 c         8061 g         7057 t
ORIGIN      
        1 ttcgtcacat catcatcggc aacgaaggtg cattgtgcta tatcactgaa gtaacagtga
       61 aaatctttaa atacaccccg gaaaacaacc tcttctacgg ctatatcctg gaagacatga
      121 aaaccggctt caacatcctg cgtgaaatca tggtggaagg gtatcgtccg tcgatcgctc
      181 gtttgtatga cgctgaagat ggcacccaac acttcaccca ttttgccgac ggaaaatgtg
      241 tgctgatctt tatggctgaa ggtaaccctc gcattgcgaa ggcgacgggc gaagggattg
      301 cggaaatcgt tgcccgctac ccgcagtgcc agcgcgtgga cagcaaactg attgaaacct
      361 ggtttaacaa cctgaactgg ggaccggata aagtggctgc cgaacgtgtg cagatcctca
      421 aaaccggcaa catgggcttt accaccgaag tgtccggctg ctggagctgc atccacgaaa
      481 tctacgaaag cgttattaac cgtattcgta ctgagttccc gcacgccgac gacatcacca
      541 tgctgggcgg tcactcctct catagctatc agaacggcac caacatgtac ttcgtctatg
      601 actacaacgt tgttgactgt aagccggaag aggaaatcga caagtaccac aatccgctca
      661 acaaaatcat ctgcgaagaa accattcgcc tcggcggttc gatggtgcac caccatggca
      721 ttggtaaaca tcgcgttcac tggagcaagc tggaacacgg cagcgcgtgg gcgttgctgg
      781 aagggctgaa aaagcagttc gatcctaacg gcattatgaa tacgggtact atctatccga
      841 ttgaaaaata atgtatcagg cagcgttccg cgatgacggg gcgttgcctt ttcggcttct
      901 caggcgagaa gccgttctta ttaccggaca atgaaggggt aaagatgaac acttcaccgg
      961 tgcgaatgga tgatttaccg cttaaccgtt ttcactgtcg aattgctacg ctcactttcg
     1021 gcgcacacct gaccgacggt tatgttctag gcgtcattgg ttacgccatt attcaactta
     1081 cgcccgccat gcaactgacg ccgtttatgg cgggaatgat cggcggctcg gcgctccttg
     1141 gtttgtttct tggcagcctg gttcttgggt ggatctccga ccatattggt cggcaaaaaa
     1201 tcttcacctt cagctttttg ctgattacgc tcgcttcgtt cttgcaattt tttgccacca
     1261 cgccagagca tcttattggg ctgcgcattt tgatcggcat tggtctggga ggcgattact
     1321 cagtaggtca caccttgctg gctgaatttt ccccgcgccg ccatcgcggt attttgctgg
     1381 gcgcattcag cgtggtgtgg accgtaggct atgtgttggc aagtattgcc ggacatcact
     1441 ttatttccga aaacccggag gcctggcgct ggttgctggc atcggcagct ctgcccgcgt
     1501 tgttgattac gttattacgc tggggaacgc cagaatcgcc acgttggcta ctgtgccagg
     1561 ggcgttttgc agaagctcac gctatcgtgc atcgctattt tggtccccat gttttactgg
     1621 gcgatgaagt ggtaacggcg acccataaac acatcaaaac cttgttctct tcgcgttact
     1681 ggcggcgcac ggcgtttaac agcgtcttct ttgtctgcct cgtaatccca tggtttgtga
     1741 tttatacctg gctgccaact atcgcccaga ctattggtct ggaagatgcg ctgactgcca
     1801 gcctgatgct taatgcgttg ttaattgtgg gcgcgctgct gggattagtt ctgacgcacc
     1861 tgctggcaca tcgcaaattt ttgctgggaa gttttttgct gctggcggca acgctggtag
     1921 taatggcctg tttgccttcc ggcagttcat taacgctgct gctttttgtt ctcttcagca
     1981 ccaccatttc ggcagtcagt aatctggtgg gcattttgcc tgcggaaagt tttcctactg
     2041 acattcgctc gctgggagtc ggttttgcca ccgccatgag tcgacttggc gcggcggtaa
     2101 gtactggcct gctgccgtgg gtgctggcgc agtggggaat gcaagtcacc ttattgctcc
     2161 tagcgacagt gttgttggtt ggttttgttg tgacctggct atgggcacca gaaactaaag
     2221 ccctcccgct ggtggcggcg ggaaatgtag gaggtgcgaa tgaacattct gttagcgttt
     2281 aaagccgaac cggatgccgg aatgctggcg gaaaaagagt ggcaggcggc ggctcagggt
     2341 aatagcggac cggatgtttc gctactgcga agtttactcg gtgctgatga acaggccgct
     2401 gccgcgctgc tgttagcgca gagaaaaaac ggtacgccga tgtctttaac cgcgttgagc
     2461 atgggggatg aacgggcgtt gcactggctg cgctatctca tggctcttgg gtttgaggaa
     2521 gctgtattgc tggagacggc ggcagatctg cgctttgcgc cggaatttgt cgctcgccat
     2581 attgccgaat ggcagcgtca gaatccgctg gatctgataa ttaccggcag ccaaagcagc
     2641 gaagggcaga atgggcaaac gccatttttg ctggcggaaa tgctgggctg gccctgcttc
     2701 acccaggtgg agcgtttcac tctcgacgcg ctgtttatca ccctcgaaca gcgtactgaa
     2761 catgggttgc gctgttgccg ggttcgcttg cctgcggtga ttgccgtgcg tcagtgcggt
     2821 gaagtggcct tgccagtacc gggaatgcgc cagcgaatgg ctgcggggaa agcagaaatt
     2881 atccgtaaaa ctgttgctgc cgaaatgcca gcgatgcagt gtctgcaact ggcaagagca
     2941 gagcaacgac gcggagcgac gctgattgat gggcaaacgg tagcagaaaa agctcaaaag
     3001 ctatggcagg actatttacg ccaaaggatg cagccatgaa tatcgcaatt gtgacaataa
     3061 atcaggagaa tgccgcaata gcgagctggc tggcggcgca ggatttcagc ggttgtacgc
     3121 tggcgcactg gcaaatagaa cctcagcccg tagtggcgga acaggtgctg gatgcgatgg
     3181 tggaacagtg gcagcgaacg cctgcggacg ttgttctctt tccgccggga acgtttggcg
     3241 atgaactgtc gacccggctg gcctggcgtt tacatggtgc aagcatctgt caggtgactt
     3301 cgttagatat tccaaccgta agcatgcgca aatcccactg gggaaatgcg ttaacggcga
     3361 cgctgcaaac agaaaaacgc ccgttatgcc tttccctggc gcgtcaggcg ggagcagcga
     3421 aaaacgccac gttgccctct ggaatgcagc aactgaacat tgtcccaggt gcgcttcccg
     3481 actggctggt tagcacagag gatctgaaaa atgttagccg cgacccatta gctgaggcca
     3541 gacgggtgct ggtggtggga cagggcggag aggcagataa ccaggaaatc gttatgctgg
     3601 cagaaaagct gggagccgaa gtgggctaca gtcgggcgcg agtgatgaac ggtggcgttg
     3661 atgctgaaaa agtgattggt atttccggac atctgctggc acctgaggtg tgcattgttg
     3721 ttggcgcttc cggcgcagca gcgttaatgg cgggcgtacg caatagcaag tttatggtgg
     3781 cgattaatca tgacgccagc gcagcggtat tttcacaggc agatattggc gtggttgatg
     3841 actggaaagt ggtgctggag gcactggtga caaacattca cgcagaatac caataacgtg
     3901 ctgccggatg atcctgccta cggttctgga ctggggacct gtaggcctga taagacgcgt
     3961 taagcgtcgc atcaggcgct gaatgtcgga tgtggcgtgc ggccaacaaa tcaaaacaaa
     4021 ttcaattggt tacgattatc ccgtcaaagc aattttttcg ctaacgtcca gactccggta
     4081 ttcgtggtgg aaagcgccac gacacccgcg ttaatcgcat tacgcgcatc ttcttcatcg
     4141 cataccagcc cgccagcaat cagcggttgg cggatttttt ccgtcaccca gcccagcact
     4201 ttgggcatgc agcctggcag gatctcaata caatccggat tcgattgcgc aacttactta
     4261 tcaatgttgt gaaacgaaat cgaatcaaca ataaacaggt gatgaataca gaaaaaaact
     4321 tccgctcttg ccgcttttag catcgatgct ttggtgctga taattccatc cgcctcggtc
     4381 accagtttca aaaactgaat caccacttct ttatttgacg cgccttccag caaatcaaca
     4441 tgaataaaag catatttccc ggcgttctta atctttttaa cgatattact gatggtgcag
     4501 atattgccgt acaacacgga aataaactgg cattcagaat cgatggccag ttgcaggctg
     4561 gcattatctt taacggcagc aatcaccgga ttctggcgga gcaggtgtaa gaggggcata
     4621 aattatcctt attatttatc caaagcggta ggtgatgccg aatccggaag gcggatagcg
     4681 ccactgttgt agtgtggatt cgtcgcacag caaacggcag gtgccacact ccaggcagcc
     4741 gcgatagtca atacgtaagt caccttcctg tgtgagagaa aaaagaccta ccggacaggc
     4801 gttaatcaac cgttgtacca tctgacgctc aacggagtca gccggaacaa tgtgcggcgc
     4861 atcagcaacg cgccagagat tacgggctac agacatcgca gactcctgat aacatcgccc
     4921 gccagatgcc acaggccatg acgacgtaaa tgatgccaga ataactggcg cagcggtgga
     4981 acaggtttat caccctgatc ccataaatcg cgggaaatat cctgcattaa cgcaggccac
     5041 gcccgatacc tgccagggcg ttgcaaaagc gccggaacat gctgataacg ttgtagaaca
     5101 tcccatagca ggctgcgctc tacgttgtga tgataaagcg caaacagatt ttgcggctcg
     5161 cggtgctggc aggcgcttat tagcgtttgt gccgccgcct gtgcgccagt cagcgccata
     5221 tccatgccgc gcacggaaat tccggtattg acgcaactgc gcaacgcatc gcccaccagc
     5281 agccagccgt taccggcgta ttgcaccggc atactgtgca agccaccttc tggcaccaga
     5341 tgcgcaccat actccagtga ttccgtgttt ttgataagcg tgcgcactgc cggatgcgct
     5401 ttaaagcgag tcagcagctc gcttgccgga acacgacttt gcgtaaggga agagagcggg
     5461 caaacaatcc ctaacgagag cgtttgttga ttagtataaa gaaatgcgcc gccgggtaag
     5521 tcatcacaga ttccgccgct gaacagcaac gctgcgcctt cgttattctc cagatgaaaa
     5581 cgttcttcaa tagcggatgt ttccagcgac agcacttctt tgatccccaa cgccatcgct
     5641 tcgccagcag gacgagtcac taacccgtga cgttcagcca ggacgctgtt ggcaccttct
     5701 gccagcacca cataacgggc gcggagaata tcgtcaccac aaataacgcc acagactctg
     5761 ccgttttctt catacagtgc atccaccgtc gctccgggga tgcattcgac accttctttt
     5821 tcggcttcgg caaccagcca cggatcgaac cgtgcacgta atacattcca ggattcaccg
     5881 ccgggctgta agctggaaaa cgtcgttgcg ccatccggcg ttaacaggga aaggctttcg
     5941 tgagtgatgc gtcgttcaag aggcgcggtc agatgaaatt gcgggaggag ttccgcgagt
     6001 gcatgggtat ataaccgcct gccggaaaga tttttgctgc cggggatttc agcgcgttcg
     6061 agtaacaaaa cggataaatc cgctcgcgcg cagcgtaacg cgcaagcggt gcctgcaata
     6121 ccggcaccaa taataataat gtcgcagtcg tcttccatgt catgccccag atatccagaa
     6181 cacaacaccc taacatagcg ttactaaagg gaaattgacc gccgacacac tcttatcatt
     6241 cgccgcgata gatacaccct gcggtgcagg tttcttttac catcaccgca cttaacagcg
     6301 gcacaacggg tttaacctga tcccaaatcc attttgctaa aacttcgctg gttggatttt
     6361 ccagacctgg aatatcattg agataatggt gatcgaggcg ctcataagtt ggcttaaacg
     6421 ccgctttcag ttcagcgaaa tcgataatcc agcccgtatg cggatcgact tccccggtaa
     6481 tttccagtcg caccataaag gaatgcccgt gcaggcgacc acatttatgc ccttccggga
     6541 catgtggtaa gcggtgagcg gcttcgaagg tgaaatcttt aaataacgtg gtggacatca
     6601 taatttctct acaggacgat aaaaccgccg tagagtaccg gaaaggctgt tttttcgcat
     6661 tatctaaaac gactttgttg cgcaaaatcg ctgatttatc ttaatgattg gctaaattca
     6721 tttgtttttc attaggttgg ttaatctatt ttgttgttaa agactattgc taaaacaggt
     6781 tagtcgattt ggttattagt tatcgctatc ccgtctttaa tccacaccgt tcaccccgtt
     6841 aaccttacct tctcttctgt tttatgggcg ctgacagggc gcagaaacag ctttgcttac
     6901 tggaacataa cgacgcatga cgacacaggt cccaccttcc gcgttgcttc cgttgaaccc
     6961 ggagcaactg gcacgccttc aggcggccac gaccgattta acgcctaccc agcttgcctg
     7021 ggtttctggc tatttctggg gcgtgctcaa ccagcagcct gttgcgcttg cagtgacgcc
     7081 agcgcccgcc gcagaaatgc cgggtataac gattatctct gcttcgcaaa ccggcaatgc
     7141 gcgccgggtt gctgaagcat tacgcgatga tttattagca gcaaaactga acgttaagct
     7201 ggtgaacgcg ggcgactata aattcaaaca aatcgccagc gaaaaactgc tcatcgtagt
     7261 gacgtcaacg caaggggaag gggaaccgcc ggaagaagcc gtcgcgctgc ataagttcct
     7321 gttctccaaa aaagcgccaa agctggaaaa caccgcgttt gccgtgttta gcctcggcga
     7381 tagctcttat gaatttttct gccagtccgg gaaagatttt gacagcaagc tggcggaact
     7441 ggggggtgaa cgcctgctcg accgtgtcga ttctgatgtt gaataccagg ctgctgccag
     7501 cgagtggcgc gcccgcgtgg ttgatgcgct taaatcgcgt gcgcctgtcg cggcaccttc
     7561 gcaatccgtc gctactggcg cggtaaatga aatccacacc agcccgtaca gcaaagacgc
     7621 gccgctggtg gctagcctct ctgttaacca gaaaattact gggcgtaact ctgaaaaaga
     7681 cgttcgccat atcgaaattg acttaggcga ctcgggcctg cgttaccagc cgggtgacgc
     7741 gctgggcgtc tggtatcaga acgatccggc actggtgaaa gaacttgtcg aactgctgtg
     7801 gctgacaggc gatgaacctg tcactgtcga gggcaaaacg ttgcctctga acgaagcgct
     7861 acagtggcac ttcgaactga ccgtcaacac cgccaacatt gttgagaatt acgccacgct
     7921 tacccgcagt gaaacactgc tgccgctggt gggcgataaa gcgaagttac agcattacgc
     7981 cgcgacgacg ctgattgttg acatggtgcg tttctccccg gcacagcttg atgccgaagc
     8041 gctaattaat ctgctgcgcc cgctgacccc gcgtctctat tccattgcct cctcgcaggc
     8101 ggaagtcgag aacgaagtac acgtcaccgt tggtgtggtg cgttacgacg tggaaggccg
     8161 cgcccgtgcc ggtggtgcct ccagcttcct cgctgaccgc gtggaagaag agggcgaagt
     8221 ccgcgtattt atcgaacata acgataactt ccgcctgccc gctaacctgg aaaccccggt
     8281 gattatgatt ggcccaggca ccggtattgc gccgttccgc gcctttatgc agcaacgcgc
     8341 cgctgacgaa gcgccaggta aaaactggct gttctttggc aatccgcatt ttacggaaga
     8401 cttcctgtat caggtggagt ggcagcgcta cgtcaaagag ggtgtgctga cgcggatcga
     8461 tctcgcctgg tcgcgcgacc aaaaagaaaa agtttacgta caagataaac tgcgcgaaca
     8521 gggcgcggag ctgtggcgct ggatcaatga cggtgcccac atttatgtct gcggcgacgc
     8581 taatcgcatg gcgaaagacg ttgagcaggc acttctggaa gtgattgccg aatttggtgg
     8641 catggacacc gaagcggcgg atgaattttt aagtgagctg cgcgtagagc gccgttatca
     8701 gcgagatgtc tactaatgag cgaaaaacat ccagggcctt tagtggtcga aggaaaactg
     8761 acagacgccg agcgcatgaa gcttgaaagc aactacctgc gcggcaccat tgcggaagat
     8821 ttaaacgacg gtctgaccgg cggctttaag ggcgataact tcctgctgat ccgcttccac
     8881 ggcatgtatc agcaggatga ccgcgacatc cgcgccgaac gtgctgaaca gaagctggag
     8941 ccgcgccacg cgatgctgtt gcgctgccgt ctgccgggtg gagttatcac caccaaacag
     9001 tggcaggcga tcgataaatt tgccggtgaa aacaccatct atggcagcat tcgcctgacc
     9061 aaccgccaga cgtttcagtt ccacggcatt ctgaaaaaga acgtcaaacc ggtgcaccag
     9121 atgctgcact cggtcggtct tgatgcgctg gcgactgcta acgacatgaa ccgtaacgta
     9181 ctctgcacct cgaaccctta cgagtcgcag ctgcacgcag aagcgtacga gtgggcgaag
     9241 aagatttctg aacatctgct gccgcgtacc cgcgcgtatg cggagatctg gctcgatcag
     9301 gaaaaagtcg ccaccaccga tgaagaaccg atccttggtc agacttacct gccgcgtaag
     9361 ttcaaaacca cggtagtgat cccgccgcag aacgatattg atctgcacgc caacgacatg
     9421 aacttcgtgg cgatcgcgga aaacggcaag ctggtgggct tcaacctgct ggtgggcggt
     9481 gggctttcta tcgaacacgg taacaagaaa acctacgccc gcacggcgag tgagtttggc
     9541 tatctgccgc tagagcatac gctagcggtg gcggaagccg tcgtgacaac tcagcgtgac
     9601 tggggtaacc gaaccgatcg taaaaatgcc aaaaccaaat acacactgga gcgcgtgggg
     9661 gttgagacgt ttaaagcgga agtggagcgt cgcgcgggga tcaaatttga accgatccgc
     9721 ccgtatgaat ttacaggacg cggcgatcgt attggctggg ttaagggcat tgatgataac
     9781 tggcacctga cgctgtttat cgaaaatggt cgcatccttg attatccggg gcgtccgctg
     9841 aaaactggcc tgctggagat cgcgaagatc cacaaaggtg atttccgcat tacggcgaac
     9901 cagaatctga tcatcgccgg tgtgccggaa agcgagaaag cgaagatcga gaaaattgcc
     9961 aaagagagcg ggttaatgaa tgccgtcacg ccgcagcgtg aaaactcgat ggcctgcgtg
    10021 tcattcccga cttgcccgct ggcgatggcg gaagcggagc gtttcctgcc gtcttttatc
    10081 gacaacatcg ataatttaat ggtgaaacat ggtgtcagcg atgagcatat cgtgatgcgt
    10141 gtaacaggct gcccgaacgg ttgtggtcgc gcgatgctgg cggaagtggg cctggtgggt
    10201 aaagcaccgg gtcgctacaa cctgcatctt ggcggcaacc gcattgggac acgtatccca
    10261 cggatgtata aagaaaacat caccgagccg gaaatcctgg cgtcgcttga tgaactgata
    10321 gggcgctggg cgaaagagcg cgaagcgggt gaaggcttcg gcgactttac ggtgcgtgcg
    10381 ggcatcattc gcccggtgct cgatccggcg cgcgatttgt gggattaacc atcagcccgg
    10441 tcttgtaggc ctgataagac gcgtgagcgt cgcatcaggc aaggcaaaca gtgaggaatc
    10501 tatgtccaaa ctcgatctaa acgccctgaa cgaactgccg aaggtagatc gcattctggc
    10561 gctggcagaa actaacgccg aactggaaaa actggacgct gaaggccgcg ttgcctgggc
    10621 gctggataat ctgcccggtg aatatgtact ttcttccagc tttggcattc aggcggcggt
    10681 gagcctgcat ctggtgaatc agattcaccc ggatattccg gtgatcctca ccgacaccgg
    10741 ctatctgttc ccggaaacct accgctttat tgacgagtta acggacaaac tcaagctcaa
    10801 cctgaaagtg taccgcgcta ccgaaagcgc cgcctggcag gaagcacgct acggcaaact
    10861 gtgggaacag ggcgttgaag gcattgaaaa gtacaatgac atcaacaaag tcgaaccgat
    10921 gaaccgggcg ctgaaagaac tgaatgcgca aacgtggttt gctggcctgc ggcgtgaaca
    10981 atccggcagc cgtgccaatt taccggtgct ggcaattcag cgtggcgtat ttaaagtgct
    11041 gccgattatc gactgggata accgaactat ttatcagtac ctgcaaaaac atggcctgaa
    11101 atatcaccca ttatgggacg aaggatattt atcggtgggc gatacccata caacccgtaa
    11161 atgggaaccc ggcatggcgg aagaagaaac gcgtttcttt ggcttaaaaa gggaatgtgg
    11221 attacacgaa gggtaaatat tgccggataa attaaacgcc tctgtcagaa atgatggggg
    11281 cgtttttatt ttgcgagcag cattccggca tttaaaggtg aacatcgcag ggaaaattaa
    11341 caggtataga atggcgttgg gtgcttgagg ctgtctggcg ttaagcattc gcgaggttcc
    11401 agatggacaa aagccccagg cgatatttct atcaacctga ggctcgcgtt cgaacgtagc
    11461 aattcggatg ttagtctctt ctttgcaggg aggcaagaca tgctgacaaa atatgccctt
    11521 gtggcaatca tcgtactgtg ttgtacagta ctgggattca cgttgatggt aggtgactcg
    11581 ttatgtgagt tgagtatcag agaacgtggt atggagttta aggcagttct cgcttacgaa
    11641 tcgaagaagt agccaccacg cgggggtaaa accccgcgta ctgaattgtg atggtttggt
    11701 ctcctgcacc ctttttttaa atttatattt tttatgtgaa tatatagtct ctatttatta
    11761 ttatgaattt caaatgaaaa tcattaattt attcataatg taaatgtttt attggctgat
    11821 atatatcata gatagttttt aaaatatgcg taaatatcct ttaagtttac tgaaggataa
    11881 aaatattgtg actttctttg atttctgggg aaaaaaccga cgtggcgaga aagagggtat
    11941 cgacgactat caccttcttt gctggcattt gctggatgtg gccgcaatgg gctatttaat
    12001 ggttaaaaga aattgcttcg ggctggctga ttattttcgt caattaggga tttctgacaa
    12061 ggaacaggca gctcgatttt tcgcctggtt gctgtgctgg cacgatattg gaaaatttgc
    12121 ccgctctttt caacaacttt acctgccccc tgaattcaag attcaggaag gtgccagaaa
    12181 aaatctctca ttcaacgctg ggctactggt tgtggaatca ttatttaagt gaatgtcagg
    12241 agttacttcc ttcatcttcg ctctctccgc gcaaacttag gcgagtgata gaaatgtgga
    12301 tgcccgtgac gacagggcat catggtcggc cccctgatcg tatggatgaa ttggataatt
    12361 ttctgcctga agacaaagct gcagcacgag attttctcct tgaaatcaag ccactgtttc
    12421 cgcgaataga gatccccaca ttctgggatg atgacgaggg cgttgaactt ataaaacaac
    12481 tttcctggta tatctctgca accgtcgtac ttgcagactg gacagggtcg tcaacgcgat
    12541 tttttccacg cgtcgcacag gcaatggata ttgaagatta ctggcaaaag gctttagttc
    12601 aggctcaaaa cgcattaacc gtctttccgc cacaagcaga aaccgcgcct ttcaccggaa
    12661 ttaatacgct gtttcctttt attgagaata cgacaccgtt acagcaaaag gtgctggatc
    12721 tggatatcaa tcagccagga ccacagttgt ttattctgga agatgtgacc ggcgcaggta
    12781 aaacagaagc ggcgcttatc ctggcgcaca ggttgatggc tgcgggtaac gctcaggggc
    12841 tgttttttgg cctgccaaca atggcaacgg ccaatgccat gtacgatcga ctggtcaaaa
    12901 cctggcttgc tttctattcg ccagaggtgt tccccgctcc agcggagata aaccggcgca
    12961 atacggtgcg gttttcccgg aagtaaaccg gacgcactgg atgcgatgat gggtatcact
    13021 tagaattccc cgcccctgcg gtagaactcc cagctcccat tttcaaaccc atcaagacgc
    13081 cttcgccagc tccttcacca acggtagcat tatccgcata acgtcacggc agcgacgttc
    13141 tattcttcca ggaagtgcct tatcaatatg ttgttgatta tccagtctta cgtcatgcca
    13201 gctatttccc gccgggaagg caggtgtttt tgcgcgttgc tgataaccat ccttatttcc
    13261 aagattccag ttagtcgctt ccaccgaaag tacagcaatg cccgctttgt cgaatatttc
    13321 tgcgtcatta caacccccag tgcctttcgg ataattttta ttcaaacccg gattggtcgt
    13381 tgcggctatt ccgtgactgc gtgcaattgc cagcgccctg tcgcgcgtta atttccttac
    13441 tgcttcaggg gtttttacac cgctgttgaa atacaattta tcgccaacaa ttaagttatc
    13501 gagattaatc accagcagcg tatttttctt ttcggtgtca ctcatccgct tgagtaaatt
    13561 ctcagcgcct aattttcctt cctcttcgcc gctggtcgcc acaaatcgaa taccatactc
    13621 ggtaggcgta tttttcaggc gttctgccag ttccagcatg acacctaaac ctgcggcgtt
    13681 atcatctatt ccttgtaacg tcagtccgcc gagattggca tctgcgtcag catcgctcag
    13741 cggggcgtaa gtatccagat gcgccataat gatgatctgc tgcggcgctt tgccttcatg
    13801 agcggcaatc accgtacttc ctgtcacgtt atgccagttc ttgcgattat cgcgggcggt
    13861 ataaatatac cgactattaa atgtccgaat atcactgcga taacccattt gctgaaactg
    13921 ttggcgaata taatcggcag ataacatttc tgctggagtt ccggtcatgc gtcccggaaa
    13981 gaaagtagca atatgtcgtg cctgggtatt agcaaaatcg ccaggtttag gtgacgaggc
    14041 gtgtacgggg agaataaagc atacgccgag cgccagggca gcggtacggt ggcgcaatgc
    14101 ggaaaacata gtgagtcctt aaataccatg caaatttttt taccgccata gtatgaaact
    14161 gccgctgcgc taaaacaatt tcaaatcttc ctaaacgccc gaaatccggt gccttaagca
    14221 ctttttgata ttagctttgc caaatcgtta ttccgttaag gaactactca ttctaattgg
    14281 taatttcatt cgttctctta cgctccctat agtcgaaaca tctgatggca agaaaatagc
    14341 ggtattgcaa aggaacggtt atggctcaaa aacgacttac tcacctgcgg caactggagg
    14401 cggaaagcat ccacattatt cgcgaggtgg cggcagaatt ctcaaatccg gtgatgctct
    14461 actctatcgg taaagattcc agcgtcatgc tgcatctggc gcgcaaggcg ttttatccag
    14521 gtacgctgcc tttcccgttg ctgcatgtcg ataccggctg gaaattccgc gagatgtatg
    14581 agttccgcga tcgtacggcg aaagcctacg gctgcgagct gctggtgcat aaaaacccgg
    14641 aaggcgtggc gatggggatt aatccattcg tgcacggcag cgcgaaacat accgatatta
    14701 tgaaaactga aggcctgaaa caggcgctga acaaatacgg ttttgatgcc gccttcggtg
    14761 gtgcgcgccg tgacgaagag aaatcccgcg ctaaagagcg aatctactct ttccgtgacc
    14821 gcttccatcg ctgggatccg aaaaatcagc gcccggagct gtggcacaac tacaacgggc
    14881 aaattaacaa aggcgaaagc atccgcgtct tcccgctctc taactggacc gagcaggata
    14941 tctggcaata catctggctg gaaaatatcg acattgtgcc gctgtatctc gctgcggaac
    15001 gtccggttct ggaacgcgac ggtatgttga tgatgattga tgacaaccgt atcgacctgc
    15061 aaccgggcga agtgatcaaa aaacggatgg tgcgtttccg tacgctgggc tgctggccgc
    15121 tgaccggtgc ggtggagtca aatgcacaaa cactgccgga aatcatcgaa gagatgctgg
    15181 tttccaccac cagtgaacgt cagggtcgcg tgattgaccg cgaccaggcg ggatccatgg
    15241 agctgaaaaa acgtcagggg tatttttaag atgaacaccg tacttgcaca acaaatcgcc
    15301 aatgaaggcg gcgtcgaagc ctggatgatt gcgcaacaac ataaaagcct gctgcgtttt
    15361 ctgacctgtg gtagcgtcga tgacggcaaa agtaccctga ttggtcgtct gctgcacgat
    15421 acccgccaaa tctacgaaga tcagctctca tcgctgcata acgacagtaa gcgtcacgga
    15481 acccagggcg aaaagctgga tctggctctg ctggtggacg gcctgcaagc tgagcgcgaa
    15541 cagggcatca ctattgatgt ggcctaccgc tatttctcta ccgagaagcg taaatttatt
    15601 atcgccgaca ccccagggca cgagcagtac actcgcaata tggcgactgg cgcatcgaca
    15661 tgtgaactgg cgatcttact gattgatgcc cgtaaaggcg tgctcgatca aacccgtcgt
    15721 cacagtttta tctccacact gttggggatc aaacatctgg tcgtggcgat caacaaaatg
    15781 gatctggtgg attacagtga agagacgttc acccgtattc gtgaagatta tctgaccttt
    15841 gccgggcagc tgccgggtaa tctggatatc cgctttgtgc cactctccgc actggaaggt
    15901 gacaacgtgg cttcgcaaag tgaaagtatg ccgtggtaca gcggtctgac actgctcgaa
    15961 gtgctggaaa ccgtggagat ccagcgagtg gtggatgccc agccaatgcg cttcccggtg
    16021 cagtacgtta accgcccaaa tctcgatttt cgtggctacg ccggaatgct ggcatccggt
    16081 cgcgtggaag tcgggcaacg tgtaaaagtg ctgccctctg gtgtggaatc aaacgtcgcg
    16141 cggatcgtga cttttgatgg tgatcgcgaa gaagtctttg ccggagaagc gatcaccctg
    16201 gtgctgacgg atgagatcga tatcagccgt ggtgatctgc tgctggcggc agacgaagcg
    16261 ttaccggcgg tgcagagtgc gtcggtggat gtggtatgga tggcggaaca gccgctttcc
    16321 ccgggccaga gttatgacat caaaattgcc ggtaagaaga cgcgtgctcg tgttgatggc
    16381 attcgttatc aggttgatat taataacctt acccaacgcg aagttgaaaa cctgccgctg
    16441 aacggcatcg gcctggtgga tctcactttt gacgagccac tggtgttaga tcgctatcag
    16501 caaaatccgg ttaccggtgg gctgattttt atcgatcgcc tgagcaatgt gaccgtgggt
    16561 gccggtatgg tgcacgagcc agttagccag gcaacagctg cgtcatctga attcagtgca
    16621 ttcgaactgg aattgaatgc cctggttcgt cgccactttc cgcactgggg cgcgcgcgat
    16681 ttgctggggg ataaataatg gcgctgcatg acgaaaacgt cgtctggcat agtcatccgg
    16741 tcactgtgca acaacgcgaa ctacaccacg gtcatcgtgg tgtagtgctg tggtttaccg
    16801 gcctctccgg gtccggtaaa tcaacggtcg ccggggcgct ggaggaggcg ttacataaac
    16861 tcagcgtcag tacgtatctg ctggatggcg acaatgttcg ccacggatta tgcagcgatc
    16921 tcggttttag cgatgccgat cgtaaagaga atatccgtcg cgtcggtgaa gtggcgaatt
    16981 tgatggttga agccggactg gtggtgctga ccgcatttat ctcgccacac cgcgccgaac
    17041 gccagatggt tcgcgaacgc gtaggagaag ggcgctttat tgaagtgttt gtcgatacgc
    17101 cgctggcgat ttgcgaagcc cgcgatccaa aaggcttata taagaaagcg cgtgccggtg
    17161 aactgcgcaa ctttacggga atagattccg tttacgaagc gcctgaatcg gcagaaattc
    17221 atctcaatgg tgaacaatta gtaacaaatt tggtacagca attattagat ctgttgagac
    17281 agaacgatat tatcagatcc tgagacacca ccgggtttcc atgcccggtt cgtcaaagta
    17341 acaggatcaa atatgcgtaa tagccataac attacactaa caaataacga cagccttact
    17401 gaggatgaag aaactacatg gtcactgcct ggtgccgtgg tcggttttat ctcctggtta
    17461 tttgcgctgg cgatgccgat gttgatttat ggctctaaca cgctgttctt ctttctctac
    17521 acctggcctt tctttctggc actgatgcct gtcgcggtag tggtggggat tgcgctgcat
    17581 tcattgatgg acggaaagct acgctacagt attgttttca ccctggtgac agtaggcatt
    17641 atgtttggcg cactgtttat gtggctactg ggctaatttg tactttcttg tctcctctgt
    17701 tcataattca aaccgtaact aataatgaga ttatgttctg cacgccctgg gtatacgtaa
    17761 caatgggcaa atgtggtaca tttgcccgcg ttgtcgcggt atccccaaca gaggatgtag
    17821 agtcgtcttc ggatgcatgg gatgatgatg cagtttttca gggggcagga tgggtaaact
    17881 aacgctgctg ttgctggcta ttctggtctg gctacagtat tcgctgtggt tcggtaagaa
    17941 cggtatacat gactataccc gcgtcaatga tgatgtggcg gcactgcaag ctacaaacgc
    18001 gaaacttaaa gcgcgaaacg atcaactttt tgccgaaatt gacgatctca atggcggcca
    18061 ggaggcgctc gaagagcgtg cgcgtaatga actcagcatg accaggccgg gcgaaacttt
    18121 ttatcgtctg gtgcctgacg cgtcgaagcg cgcacagtct gcggggcaaa acaatcgata
    18181 aatcagcccg ggaattaaca tggcaaccac tcatttggat gtttgcgccg tggttccggc
    18241 ggccggattt ggccgtcgaa tgcaaacgga atgtcctaag caatatctct caatcggtaa
    18301 tcaaaccatt cttgaacact cggtgcatgc gctgctggcg catccccggg tgacacgtgt
    18361 cgtcattgcc ataagtcctg gcgatagccg ttttgcacaa cttcctctgg cgaatcatcc
    18421 gcaaatcacc gttgtagatg gcggtgatga gcgtgccgat tccgtgctgg caggtctgaa
    18481 agccgctggc gatgtgcagt gggtattggt gcatgacgcc gctcgtcctt gtttgcatca
    18541 ggatgacctt gcgcgattgt tggcgttgag cgaaaccagc cgcacgggag ggatcctcgc
    18601 cgcaccagtg cgcgatacta tgaaacgtgc cgaaccgggc aaaaatgcca ttgctcatac
    18661 cgttgatcgc aacggcttat ggcacgcgct gacgccgcaa tttttccctc gtgagctgtt
    18721 acatgactgt ctgacgcgcg ctctaaatga aggcgctacc attaccgacg aagcctcggc
    18781 gctggaatat tgcggatttc atccgcagtt ggtcgaaggc cgcgcggata acattaaagt
    18841 cacgcgcccg gaagatttgg cactggccga gttttacctc acccgaacca tccatcagga
    18901 gaatacataa tgcgaattgg acacggtttt gacgtacacg cctttggcgg tgaaggccca
    18961 attatcattg gtggcgtacg cattccttac gaaaaaggat tgctggcgca ttctgatggc
    19021 gacgtggcgc ttcatgcgtt gaccgatgca ttgcttggcg cggcggcgct gggggatatc
    19081 ggcaaactgt tcccggatac cgatccggca tttaaaggtg ccgacagccg cgagctgcta
    19141 cgcgaagcct ggcgtcgtat tcaggcgaag ggttataccc ttggcaacgt cgatgtcact
    19201 atcatcgctc aggcaccgaa gatgttgccg cacattccac aaatgcgcgt gtttattgcc
    19261 gaagatctcg gctgccatat ggatgatgtt aacgtgaaag ccactactac ggaaaaactt
    19321 ggatttaccg gacgtgggga agggattgcc tgtgaagcgg tggcgctact cattaaggca
    19381 acaaaatgat tgagtttgat aatctcactt acctccacgg taaaccgcaa ggcaccgggc
    19441 tgctgaaagc caatccggaa gactttgtgg tggtggaaga tttgggcttt gagcctgatg
    19501 gtgaaggtga gcatattctg gttagaatcc tcaaaaacgg ctgcaatacc cgttttgtgg
    19561 cggatgcact ggcgaaattc ctgaaaattc attcccgtga agtcagcttc gctgggcaaa
    19621 aagacaaaca tgctgtaacg gaacagtggt tatgtgctcg cgtgccgggc aaggaaatgc
    19681 ccgatctgag cgcctttcaa ctggaaggct gccaggtgct ggagtatgcg cggcacaagc
    19741 gcaagctgcg tttaggggcg ctgaaaggta acgcctttac cctggttctg cgcgaagtga
    19801 gcaatcgcga tgacgttgaa caacgtctga tcgatatttg cgtaaaaggt gtaccgaact
    19861 acttcggtgc ccaacgtttt gggattggcg gtagcaactt gcagggcgcg ctgcgctggg
    19921 cgcaaaccaa tactccggtg cgcgatcgca ataaacggag tttttggttg tcggcagccc
    19981 gcagtgcgtt gtttaatcag attgttgctg agcgcctcaa aaaagcagac gttaatcaag
    20041 ttgttgacgg cgatgcgcta caattagccg gacgtggtag ctggtttgta gcaaccaccg
    20101 aagaactggc ggaattacag cgtcgcgtca acgataaaga gctgatgata accgccgcat
    20161 tgccgggcag tggcgaatgg ggaactcagc gtgaagcgct ggcattcgaa caagcagctg
    20221 tcgccgcaga aactgaatta caagctttac tggtgcgcga aaaagttgaa gccgcgcgca
    20281 gagcgatgct gctgtatccg caacaattaa gctggaactg gtgggatgac gtcaccgtag
    20341 agatccgttt ctggcttccg gcgggtagtt ttgcaaccag cgttgtcagg gaacttatca
    20401 acacaacagg tgattatgcg catattgctg agtaattatg acggggtaca tgcacccggt
    20461 atacaaacgc tggcgaaagc cttgcgtgag tttgctgacg ttcaggtggt cgcccccgat
    20521 cgtaaccgca gcggcgcttc aaattctctg acactggaat cctccctgcg cacgtttacc
    20581 tttgaaaatg gtgatattgc tgtgcaaatg ggaaccccga ccgattgtgt ctatcttggc
    20641 gtgaatgctc tgatgcgtcc gcgcccggac attgttgtgt ccggaattaa cgccgggccg
    20701 aatctggggg atgatgttat ttattccggt acggtagccg ccgcgatgga aggccgtcat
    20761 ttaggttttc cggcgctggc cgtctcgctt gacgggcata aacattacga cactgccgcg
    20821 gcggtaacct gttcaatttt gcgcgcactg tgtaaagagc cgctgcgcac cggacgtatt
    20881 cttaatatta acgttcagga tttacccttg gatcaaatca aaggtattcg cgtgacgcgc
    20941 tgcggtacac gacatccggc agatcaggtg atcccgcagc aagatccgcg cggcaatacg
    21001 ctgtactgga ttggcccgcc gggcggtaaa tgtgatgccg gtccggggac cgattttgct
    21061 gcggtagatg agggctatgt ctccatcacg ccgctgcatg tggatttaac tgcgcatagc
    21121 gcgcaagatg tggtttcaga ctggttaaac agcgtgggag ttggcacgca atggtaagca
    21181 gacgcgtaca agcacttctg gatcaattac gtgcgcaagg tattcaggat gagcaggtgc
    21241 tgaatgcact tgccgccgtg ccgcgtgaaa aattcgttga tgaagcgttt gaacaaaaag
    21301 cctgggacaa tatcgccttg ccgataggtc aggggcagac aatttcgcag ccgtatatgg
    21361 tggcgcgaat gaccgaatta ctcgagctga cgccgcagtc gcgggtgctg gaaattggca
    21421 ctggttcggg atatcaaacg gcaatcctgg cgcatcttgt ccagcatgtt tgctcggttg
    21481 aacggattaa aggcttgcag tggcaggcac gtcgccgcct gaaaaatctt gatttacata
    21541 atgtttcaac ccgtcatggt gatggatggc aaggttggca ggcacgtgcg ccgtttgacg
    21601 ctatcattgt tacggcggca ccgccggaaa ttccaactgc gctaatgacg cagctggacg
    21661 aaggcgggat tctcgtctta cccgtagggg aggagcacca gtatttgaaa cgggtgcgtc
    21721 gtcggggagg cgaatttatt atcgataccg tggaggccgt gcgctttgtc cctttagtga
    21781 agggtgagct ggcttaaaac gtgaggaaat acctggattt ttcctggtta ttttgccgta
    21841 ggtcagcgta tcgtgaacat cttttccagt gttcagtagg gtgccttgca cggtaattat
    21901 gtcactggtt attaaccaat ttttcctggg ggataaatga gcgcgggaag cccaaaattc
    21961 accgttcgcc gcattgcggc tttgtcactg gtttcgctat ggctggcagg ctgttctgac
    22021 acttcaaatc caccggcacc ggtcagctcc gttaatggca atgcgcctgc aaatactaat
    22081 tctggtatgt tgattacgcc gccgccgaaa atggggacga cgtctacagc gcagcaaccg
    22141 caaattcagc cggtacagca gccacaaatt caggctactc aacaaccgca aatccagcca
    22201 gtgcagccag tagctcagca gccggtacag atggaaaacg gacgcatcgt ctataaccgt
    22261 cagtatggga acattccgaa aggcagttat agcggcagta cctataccgt gaaaaaaggc
    22321 gacacacttt tctatatcgc ctggattact ggcaacgatt tccgtgacct tgctcagcgc
    22381 aacaatattc aggcaccata cgcgctgaac gttggtcaga ccttgcaggt gggtaatgct
    22441 tccggtacgc caatcactgg cggaaatgcc attacccagg ccgacgcagc agagcaagga
    22501 gttgtgatca agcctgcaca aaattccacc gttgctgttg cgtcgcaacc gacaattacg
    22561 tattctgagt cttcgggtga acagagtgct aacaaaatgt tgccgaacaa caagccaact
    22621 gcgaccacgg tcacagcgcc tgtaacggta ccaacagcaa gcacaaccga gccgactgtc
    22681 agcagtacat caaccagtac gcctatctcc acctggcgct ggccgactga gggcaaagtg
    22741 atcgaaacct ttggcgcttc tgaggggggc aacaagggga ttgatatcgc aggcagcaaa
    22801 ggacaggcaa ttatcgcgac cgcagatggc cgcgttgttt atgctggtaa cgcgctgcgc
    22861 ggctacggta atctgattat catcaaacat aatgatgatt acctgagtgc ctacgcccat
    22921 aacgacacaa tgctggtccg ggaacaacaa gaagtgaagg cggggcaaaa aatagcaacc
    22981 atgggtagca ccggaaccag ttcaacacgc ttgcattttg aaattcgtta caaggggaaa
    23041 tccgtaaacc cgctgcgtta tttgccgcag cgataaatcg gcggaaccag gcttttgctt
    23101 gaatgttccg tcaagggatc acgggtagga gccaccttat gagtcagaat acgctgaaag
    23161 ttcatgattt aaatgaagat gcggaatttg atgagaacgg agttgaggtt tttgacgaaa
    23221 aggccttagt agaagaggaa cccagtgata acgatttggc cgaagaggaa ctgttatcgc
    23281 agggagccac acagcgtgtg ttggacgcga ctcagcttta ccttggtgag attggttatt
    23341 caccactgtt aacggccgaa gaagaagttt attttgcgcg tcgcgcactg cgtggagatg
    23401 tcgcctctcg ccgccggatg atcgagagta acttgcgtct ggtggtaaaa attgcccgcc
    23461 gttatggcaa tcgtggtctg gcgttgctgg accttatcga agagggcaac ttggggctga
    23521 tccgcgcggt agagaagttt gacccggaac gtggtttccg cttctcaaca tacgcaacct
    23581 ggtggattcg ccagacgatt gaacgggcga ttatgaacca aacccgtact attcgtttgc
    23641 cgattcacat cgtaaaggag ctgaacgttt acctgcgaac cgcacgtgag ttgtcccata
    23701 agctggacca tgaaccaagt gcggaagaga tcgcagagca actggataag ccagttgatg
    23761 acgtcagccg tatgcttcgt cttaacgagc gcattacctc ggtagacacc ccgctgggtg
    23821 gtgattccga aaaagcgttg ctggacatcc tggccgatga aaaagagaac ggtccggaag
    23881 ataccacgca agatgacgat atgaagcaga gcatcgtcaa atggctgttc gagctgaacg
    23941 ccaaacagcg tgaagtactg gcacgtcgat tcggtttgct ggggtatgaa gcggcaacac
    24001 tggaagatgt aggtcgtgaa attggcctca cccgtgaacg tgttcgccag attcaggttg
    24061 aaggcctgcg ccgtttgcgc gaaatcctgc aaacgcaggg gctgaatatc gaagcgctgt
    24121 tccgcaagta agtaagcatc tgtcagaaag gccagtctca agcgaggctg gcctttttct
    24181 tttgtttggg aagcttgtgt ttttgtcgcc tggataagac acgtcagcgt cctacccggc
    24241 aatcagcatt ctatgtgctc atcatcttgc gcaccaactg cataaacagc tcctgctctt
    24301 ctccactcaa acgccccaaa aattcctcat ccacgctgtc accaataggt atcgccgcag
    24361 caagtagctt ttctccttcg gcagtcagcc agacaaagcg tcgccgcttg tcagcgacgt
    24421 catgttcacg tctgactaag ccacgatttt ccattcttgc caacatttct gctagcgttg
    24481 ctttggtgct gactgctgct tctatcagtg ccacctgttc aataccaggt ttatcagcaa
    24541 tggcgcgcat caccgcgtac tgtggtttgg tcaggtcagg taactcgtgc tgccagcgag
    24601 ccgtgtgctg ctgaaaaagc tgtcgtaact gatggaacgc tttatttcgt aacgccatgt
    24661 aaactccggg ctaattctct gtcgctatca tagcggtttt ttagtgacga agaagatgtt
    24721 gttacttttc aatccttgcc gctgtggaaa tgaaggagta tgttaataat aacgttcgta
    24781 tacgaataat taagagaata agcaatgaaa ctgatcgtcg ggatgacagg ggctaccggt
    24841 gcgcctcttg gtgtggcatt actgcaagcg ctgcgtgaga tgccgaatgt cgagactcat
    24901 ctggtgatgt cgaagtgggc gaaaaccacc attgaactgg aaacgcctta cagcgctcgc
    24961 gatgttgctg ccctcgcaga cttcagccat aacccggcgg atcaggcggc gatcatctca
    25021 tccggttctt ttcgtaccga cggcatgatc gttattccgt gcagtatgaa aacgctcgcc
    25081 ggtatccgcg ctggttacgc cgatggcctg gtagggcgcg cggcggacgt cgtgctcaaa
    25141 gaaggccgca aactggtgct ggtgccgcgt gaaatgccgc ttagcaccat ccatctcgaa
    25201 aatatgctcg cactttcacg catgggcgtg gcgatggtgc cgccgatgcc tgccttttat
    25261 aaccatcccg aaacggtaga tgacattgtc caccatgtgg tagcccgcgt gctggatcaa
    25321 tttggcctcg aacatcccca cgccaggcgc tggcaaggat tgccgcaggc ccggaatttt
    25381 tctcaggaga atgaataatg gcatttgatg atttacgcag ctttttacag gcgcttgatg
    25441 accacggcca gttactgaaa atcagcgaag aagtgaacgc tgagccggat ctggcagcag
    25501 cagctaacgc caccgggcgt atcggcgacg gcgcgcccgc gctgtggttt gataatattc
    25561 gcggctttac cgatgcccgc gtggcgatga ataccatcgg ttcctggcag aaccacgcga
    25621 tttcccttgg cctgccacca aacaccccgg tcaaaaagca gattgatgag tttatccgcc
    25681 gctgggataa cttcccgatt gccccggagc gccgcgccaa tccagcctgg gcgcagaaca
    25741 ccgtcgatgg cgaagagatc aacctgttcg atattctgcc gctgtttcgc ctgaatgatg
    25801 gcgatggcgg tttctatctc gacaaagcgt gcgtggtttc ccgcgatccg ctcgacccgg
    25861 ataacttcgg caagcagaac gtcggcatct accgcatgga agtgaagggc aagcgtaagc
    25921 tcggcctgca accggtgccg atgcacgata tcgccctgca tctgcataaa gcagaagagc
    25981 gcggtgaaga tctgccgatt gcgatcacgc tcggtaacga tccgatcatc acgctgatgg
    26041 gggccacgcc gctgaaatac gatcagtctg agtatgaaat ggcaggcgcg ctgcgtgaaa
    26101 gcccgtaccc gatcgccacc gctccgctga ccggttttga tgtgccgtgg ggttcagaag
    26161 tgatcctcga aggggttatc gaaagccgta aacgcgaaat cgaagggccg ttcggtgagt
    26221 ttaccgggca ctactccggt gggcgcaaca tgaccgtggt gcgcatcgat aaagtctctt
    26281 atcgcagcaa accgattttc gaatcactct atctcggtat gccgtggacc gaaatcgact
    26341 acctatgact aggtaattta ttcaagataa ccgggaagag attaaaaaag acgggaatag
    26401 attggaatca acaagttagg aactcaaggt tttcaagata aagcgagtcc aaaaatgtta
    26461 aacggttcga tatcaataaa acattcaaga taactcacag gttcttacaa tagcctttaa
    26521 agaccgttca aaaacgattc aggtgtttat tatagcgctg atgggtgcaa gcgccaaaac
    26581 caatgtaatt ttagcacagc taggcacttg cactgggtta tgcgctggct gctttctgag
    26641 gtagatttaa gtgatcgccc ttagcttcgg acaacgcgta aatctctcta accttttcgt
    26701 ctggcaaatt accaaggctt aacgctatgg ctctacgaac tccaactaga gcgtgcaact
    26761 cttggatgtg ctcatccaga agaatgagta attgctccgc tccttttctc ccaagaacat
    26821 cagctacttg ttgtttttct tgtggtttaa gaaatgacaa gaacattaga atttgagcat
    26881 caacattatc cgcattctct tttttgggtt ctttggaatc ttttagttca acatcaccct
    26941 ctcccgtcat gagccattcc aaggtaacat tttcgctttc tgctatcttt tttaactgat
    27001 acgggcgtgg tatcggtgcg taatcccgcc tgttgtaata attttttaaa gtgccttcat
    27061 tgatccccca tgcccgagca gccgcatttc ttgaagggta tcgttctact aacctaaaga
    27121 ttctttcgat aatcggttct tttccatcgg caggaaaaga accttggggt gcttttctca
    27181 ttcttggttc ctttctatct aattcattga taaataataa aattaaaaaa taaaccataa
    27241 aaacaccaaa gaaccctttg cagggaatct ttggtatctt aatctttatc catacggata
    27301 tccgaccgga tatccgcgcg ggtaattttg taggttaaag gaaagatgag acaaagagaa
    27361 gatagtgacc gcgattggcc ttcgctgaag attcagctgg cgctcaaaga taacgggtgg
    27421 tcttccttcc gggagctttc actccatgct ggttatgctc aggattcact gaaagcggta
    27481 gttcgcacac caaacaaaaa atacgaacgc gtaatttctg acgctatagg tgtaccgcca
    27541 gaaatcatct ggcccagccg gtatcgtaac gatggattta tcaggaaggc gtcgtgatat
    27601 gcagtcgtac gttaccgtaa atgacctgct cggcgtacca ggaatgccag ccaccacaaa
    27661 aggcatccga caggcgttac agcgtttttc tcgtgatctt ggtgacgtta gtcgccgtcg
    27721 ggaaggcaca aaagccattg agtaccacat tgattgtctg cctgaaataa cacgaaaggc
    27781 actgcgtgaa cgctatgtgg aacagctggt ggctacagag aacaatgttt ctgaagtgaa
    27841 agctgttacc agaaaaacac gcaatcctga cgctgtccag gcaatcgaag catatcgcgg
    27901 ttctccacaa ctgatggaag aacgcctgaa tgcgctgacc gaaaaccagc gctgggtatc
    27961 tgaagcaaga gctgcgctgg tggtggaagt gctgaagctg gaaagcgccg gtaaccccgg
    28021 gcgactgaaa gccattaact ttcttgttga aaaagcccgt aaaggtgagc tgccggagcg
    28081 cctgcaacag gccgcagtta acgccaatgc aaaacgtggc gctaatcgta ctatcagccg
    28141 cgatccactt taccagtggg ttctgaaata caaccagtcg cagaacgccg ccgaacggct
    28201 gctgttactg gcaccgggaa aacgcgacga aataaaaccg gaagaaatca gctggctgcc
    28261 tgaatttctg gcgcagtatc gccaggtgaa cggcagaccc atgtcagaag cctacgagga
    28321 ttttgtcgca gaatggcagc gtcgccatgc ggacgaaccg tacatgctgg aggtgatgcc
    28381 ttcctatgat gttgtccggt atgccatgaa aaaactgccg gaagtggtga aacaaaaagg
    28441 gcgcgttacc ggcagtgaat accgtcagct tgaaggattc acccgccgcg actggacagc
    28501 tatgccggtg aattatgtct ggattggtga cggtcacggt atgaagctga aatgtgcgca
    28561 tccgatccac gggcgtccat tctcaccgga agtcacgttt gtgatcgatg gtggaacacg
    28621 gtttgttgtt ggctggagtc ttgaccttgc cgaaaacgtg ttcgccgttg ccggtgcgat
    28681 acagcacggc attcgcaacc acggaaaacc gttcctgtat tactcggata acggttccgg
    28741 tgaaacggct gacatgctgg acaaagaggt cgtgggtatt ctgccacgcc tggggattaa
    28801 acaccctacc ggtattgccg gtaatccaca gggacgcggc attattgaac gcctgaaccg
    28861 gacactgccg atgcgtatag cgaggaggta tcgcacctat tttggcaagg gtgctgaccg
    28921 ggaatcgttg cgtgtactta accgtgatct gcgttcagcg tttaacgccc tgcaacagga
    28981 caagcctctg aacgaccgcc agaaagcggc aatgcgcgag ctgccatcgt gggcggagct
    29041 tattgaggct attcgtgaag gcgtggaatg gtacaacaac cgcccgcatt ctgagctgcc
    29101 gatgaaaccg aacggacggc attacagccc gacagagttc agaaaaaaac gccaggcaga
    29161 agaagacacc gaaattgagt ggctgagtga tcttgagctg cgcgacatgt tccgcccaat
    29221 ggtggaacga ccggtaagac gctgcgaaat tcagtggctg aacaatattt actacgcgcc
    29281 ggagttgcgt gacgaacacg gtcgcaaagt gcttatcagt tatgacattc atgacgccga
    29341 gcgaattacg gtacgtcgca aggacggcag ctttatctgt gaggcgatat ggaacggcaa
    29401 taaacgcgca gcctttgctg tcagtgctga atatcacaaa caacagcagc gtattaaagg
    29461 aatgcgcaaa cgcgctgagg aaaaaattcg tgatgctgaa gatgaaggta ttcagattct
    29521 ggaacacaaa caggctgaac cctggctgag taatgtttat cgcccggttg gtaatgtggt
    29581 ggcagttcag caaccggaat atgaagaaga gcatgacgaa gaatttgaac gcgatttccg
    29641 gctggggatg caaaaacttt ttgccatgca ggaagaagat gatccgctgg cctgaataaa
    29701 aaaacagtcc gcgggaacgg actgttaaat aaaggaaaca aagctgattt gaaataaccg
    29761 aggaaatact atgactgata ttgatgat
//
LOCUS       DAZSIO010000033        29746 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZSIO010000033 DAZSIO010000000
VERSION     DAZSIO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.033"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..1224
                     /locus_tag="ACHX5T_001341"
     CDS             <1..1224
                     /locus_tag="ACHX5T_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR8466606.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="glpX"
                     /locus_tag="ACHX5T_001342"
     CDS             1221..2186
                     /gene="glpX"
                     /locus_tag="ACHX5T_001342"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR8466607.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="ACHX5T_001343"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="ACHX5T_001343"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFR8466608.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="ACHX5T_001344"
     CDS             2714..3427
                     /locus_tag="ACHX5T_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR8466609.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="ACHX5T_001345"
     CDS             complement(3399..4076)
                     /locus_tag="ACHX5T_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8466610.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="ACHX5T_001346"
     CDS             complement(4070..4747)
                     /locus_tag="ACHX5T_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8466611.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5442)
                     /locus_tag="ACHX5T_001347"
                     /pseudo
     CDS             complement(4735..5442)
                     /locus_tag="ACHX5T_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
     gene            complement(5443..6021)
                     /locus_tag="ACHX5T_001348"
     CDS             complement(5443..6021)
                     /locus_tag="ACHX5T_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR8466612.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="ACHX5T_001349"
                     /pseudo
     CDS             complement(6044..6475)
                     /locus_tag="ACHX5T_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="ACHX5T_001350"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="ACHX5T_001350"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR8466613.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="ACHX5T_001351"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="ACHX5T_001351"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR8466614.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="ACHX5T_001352"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="ACHX5T_001352"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR8466615.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /gene="mscS"
                     /locus_tag="ACHX5T_001353"
     CDS             10564..11424
                     /gene="mscS"
                     /locus_tag="ACHX5T_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR8466616.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="ACHX5T_001354"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="ACHX5T_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR8466617.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="ACHX5T_001355"
     CDS             12291..13031
                     /locus_tag="ACHX5T_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR8466618.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14148
                     /locus_tag="ACHX5T_001356"
     CDS             13198..14148
                     /locus_tag="ACHX5T_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8466619.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACHX5T_001357"
                     /pseudo
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACHX5T_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACHX5T_001358"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACHX5T_001358"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR8466620.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACHX5T_001359"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACHX5T_001359"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR8466621.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACHX5T_001360"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACHX5T_001360"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR8466622.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACHX5T_001361"
     CDS             complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACHX5T_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR8466623.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPELFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="ACHX5T_001362"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="ACHX5T_001362"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR8466624.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /gene="serA"
                     /locus_tag="ACHX5T_001363"
     CDS             21915..23147
                     /gene="serA"
                     /locus_tag="ACHX5T_001363"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR8466625.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="ACHX5T_001364"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="ACHX5T_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR8466626.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="ACHX5T_001365"
     CDS             23768..23827
                     /locus_tag="ACHX5T_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR8466627.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23928..24530)
                     /locus_tag="ACHX5T_001366"
     CDS             complement(23928..24530)
                     /locus_tag="ACHX5T_001366"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR8466628.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24551..24734)
                     /gene="ssrS"
                     /locus_tag="ACHX5T_001367"
     ncRNA           complement(24551..24734)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHX5T_001367"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="ACHX5T_001368"
     CDS             complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="ACHX5T_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR8466629.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25273..25851
                     /gene="ygfB"
                     /locus_tag="ACHX5T_001369"
     CDS             25273..25851
                     /gene="ygfB"
                     /locus_tag="ACHX5T_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR8466630.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25877..27202
                     /gene="pepP"
                     /locus_tag="ACHX5T_001370"
     CDS             25877..27202
                     /gene="pepP"
                     /locus_tag="ACHX5T_001370"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR8466631.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27199..28377
                     /gene="ubiH"
                     /locus_tag="ACHX5T_001371"
                     /gene_synonym="visB"
     CDS             27199..28377
                     /gene="ubiH"
                     /locus_tag="ACHX5T_001371"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR8466632.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28400..29602
                     /gene="ubiI"
                     /locus_tag="ACHX5T_001372"
                     /gene_synonym="visC"
     CDS             28400..29602
                     /gene="ubiI"
                     /locus_tag="ACHX5T_001372"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR8466633.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29676..>29746)
                     /locus_tag="ACHX5T_001373"
     CDS             complement(29676..>29746)
                     /locus_tag="ACHX5T_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466634.1"
                     /translation="KSKNHGIRKYNAKTEVKLVYFK"
BASE COUNT         7102 a         7437 c         8027 g         7180 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa actgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcaggcgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggggctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agccgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ttatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggtgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgatcacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc taggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgagta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcacttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ctggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcgcattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgcgattg ggctttctcg tttctcgccg ttatgaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggcgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgtg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagct
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgcaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg actgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttgttgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgatgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt tttctacggc taggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggt ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tcaattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttatcc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttagacat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagctc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caggcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gtcaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcagtcacag gggaggagca atcctccctt acccttactc actaaattag
    23881 gttaaagaat caacgatgtc aatcagggcg atgcaggtgt atcgccctta ccactcccag
    23941 actttcgacg gtgtaaccac cgcaggaaga gggatatccc actcttcaac ggggagtttt
    24001 tccaccaact gacaatcatg cgcataaccc accggttgcg ttttatagtg ctgccagttt
    24061 tgtaaggtcc gatcataaaa accaccgccc attcccagac gctgaccgta ctcatcaaag
    24121 gcgaccagcg gtgtgatcag cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc
    24181 agttttggct catggatctt caacctgttc atcaccagtt cgctttgcgg atggtaattc
    24241 aggaacagca aattaccggc actaaaggga tgcaaaactg gaagatatac gcgcttaccg
    24301 gcgcgccaga gttgttctat tagcggctgg gtgtcgagtt caccatcaaa agagaggaat
    24361 acagcgaccg tatgcgccat caccaccggg ggataagtca tcatccgggt agcggcttgt
    24421 tgacccattt ccagctgttg ttccggcgtt aacgcacgac gacgttgccg aatcattttg
    24481 cggatttctt gtcgggataa tgtcagtggg agctctggta gttgcgtcat ggctggtgcc
    24541 agataagaag ggaatctccg agatgccgcc gcaggctgta acccttgaac ccttggttca
    24601 aggtgaatgt gtcgtcgcag ttttaaggct tctcggacga accgagcatg ctcaccaacc
    24661 gcggagcgcc acattcttgt ggtatgaaat atcggctcag gggactggcc cgcttgcgaa
    24721 catctcagag aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt
    24781 caaagttttg gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg
    24841 tctgctgcag catccgaata cgctgttcca tacttgccgc gtagtcacga gtctttgctt
    24901 tttcttgcgc taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat
    24961 ttgtgactct agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgctgcct
    25021 gattcaacgc atccctttgg tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt
    25081 ggatatcgac gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt
    25141 ctttagaccc atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag
    25201 cccttttctg gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga
    25261 cgacgaatgc ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat
    25321 ctgaaccaac aagggacggg tctgacccct gccgagatgc atggtttaat cagtgggatg
    25381 atatgtggcg gtaacgatga cagctcatgg ctgccgctac ttcacgacct gacgaacgaa
    25441 ggcatggctt tcggtcatga gctggcacag gcactgcgta aaatgcactc tgccaccagc
    25501 gatgccctgc aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc
    25561 agcgttttcg atcgggctga tgcactggca ggttgggtca atcacttcct gcttggtctt
    25621 ggcgttacgc aaccgaagct ggacaaagtg accggcgaaa ccggtgaagc catcgacgat
    25681 ctgcgtaaca tcgcgcagct gggctacgac gaagacgaag atcaggaaga gcttgaaatg
    25741 tcgcttgaag agatcatcga atacgttcgt gttgccgcgc tgttatgcca cgacaccttt
    25801 actcatccgc aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa
    25861 acgtaaggag agtgttatga gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc
    25921 cctggtggag caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac
    25981 acgtagcgcc gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg
    26041 ctttaacgaa ccggaagcgg tgctggtgct gattaaaagc gatgacactc ataaccacag
    26101 cgttctgttt aaccgcgttc gcgacctgac ggcggagatc tggtttggcc gtcgcttagg
    26161 ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa
    26221 tcagcaactt tatcaactac ttaacggcct ggatgtggtt taccatgccc agggcgaata
    26281 tgcatatgct gatgaaatcg tgaacagtgc gctggaaaaa ctgcgtaaag gttcgcggca
    26341 aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct
    26401 gttcaaatcg ccagaagaga ttgccgtact ccgccgcgcg ggagaaatca ccgccatggc
    26461 acatacacgg gcgatggaaa aatgccgtcc gggaatgttc gagtaccatc tggaaggcga
    26521 aattcaccac gaatttaacc gccacggtgc gcgctatcct tcctataaca ccattgtagg
    26581 cagcggtgaa aacggctgca ttctgcacta caccgaaaac gagagtgaac tgcgcgacgg
    26641 tgacctggtg ttgattgacg cgggttgtga atacaaaggt tacgcgggcg atattacccg
    26701 caccttcccg gtcaacggca aattcaccca ggcccagcgt gaaatctacg acattgtgct
    26761 ggagtctctc gaaaccagcc tgcgcctgta tcgtccggga acttccattc tggaagtcac
    26821 tggtgaagtg gtgcgcatca tggttagcgg cctggtaaaa ctcggcatcc tgaaaggtaa
    26881 tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca
    26941 ctggttagga ctggatgtcc atgacgttgg cgtttatggt caggatcgct cgcgcattct
    27001 ggaaccgggg atggtactga ccgtagagcc tggtctgtat atcgcgccgg atgcggatgt
    27061 gccagaacaa tatcgcggta tcggcattcg tattgaagac gacattgtga ttaccgaaac
    27121 cggcaacgaa aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat
    27181 ggctgctgcg agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg
    27241 ctggcgctgg ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg
    27301 actgcgccag agtcacatgc tcatccgggc tttgatggac gagcgatagc gctggcggcg
    27361 ggtacctgtc agcaactggc gcgcatcggc gtctggcaat ctctggctga ttgcgcaacc
    27421 gccatcacta ccgtgcatgt cagcgatcgt ggtcatgctg gattcgtcac cctcgccgca
    27481 gaagattacc aactggcggc gctggggcag gttgtcgaat tgcataatgt cgggcaacgg
    27541 ctgtttgcat tgctgcgaaa agcacctggc gtaatgctgc attgccccga tcgcgtagct
    27601 aacgttgccc gtactcagag tcatgttgaa gtgacgctgg agagcggcga gatgctgacg
    27661 ggccgcgtgc tgatagccgc tgatggcacc cattcagcgt tagccaccgc gtgcggcgtt
    27721 gattggcagc aggagcctta cgaacaactg gcagtgattg ccaacgttgc tacttctgtt
    27781 gcgcacgaag gccgcgcttt tgaacgcttc acgcaacatg gcccgctggc gatgttgccg
    27841 atgtctgacg gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggtg
    27901 ctgtcgtgga gcgacgagaa gttttgccgt gaactccagt ctgcctttgg ctggcgactg
    27961 gggcaaatta cccacgccgg taaacgcagt gcttatccgc tggcattaac ccgcgccgcc
    28021 agaccgatta cccatcgcac tgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt
    28081 gccgggcaag ggtttaacct cggtatgcga gatgtcatga gccttgcgga aaccctgact
    28141 caggcgcatg agcgcggaga agacatcggt gattacggca tattgtgccg ctatcagcag
    28201 cgtcgacaga gcgatcgcga agcaaccatc ggcgtcacgg acagcctggt acatcttttt
    28261 gccaaccgtt gggcaccgct ggttgtcggg cgcaacatcg gactgatgac gatggaatta
    28321 ttcaccccgg cacgcgatgt gctggcgcag cgcaccctcg gttgggtggc gcgttgaagg
    28381 cggttttaag gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt
    28441 ggggctggcg gttgcctgcg gcttacaggg gagcggctta cgcgttgccg tactggagca
    28501 gcgcgtaccg gaacctctgg cggtagatgc actaccacaa ctgcgcgttt cggctatcaa
    28561 tgccgccagc gaaaaattac tcacccgtct tggcgtctgg caggacattc tcgctcgtag
    28621 ggccagttgt tatcacggca tggaagtgtg ggacaaagac agttttggtc acatctcgtt
    28681 tgacgatcaa agcatgggct atagccatct tgggcacatc gttgaaaact cagtgattca
    28741 ctacgcgctg tggaacaaag cgcagcagtc gtcagatatt actctgttgg cccccacaga
    28801 attacagcag gtcgcctggg gagaaaatga aaccttcctg acgctgaaag atggcagcat
    28861 gttaacggcg cgtctggtga ttggcgcgga cggtgctaat tcctggttgc gcaacaaagc
    28921 agatattccg ctgactttct gggattatca gcatcacgcg ctggtagcga ccattcgcac
    28981 ggaagaaccg catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt
    29041 tttaccgctt agcgatccgc atctttgctc gattgtctgg tcactgtcgc cagaggaagc
    29101 gcagcggatg cagcaggcaa gtgaagacga atttaatcgc gcgttaaata tcgcttttga
    29161 taatcgcctt ggcttatgca aggttgagag cgcgcgtcag gtgttcccgc tgacggggcg
    29221 ttatgcgcgc cagtttgccg cacaccgtct ggcgctggtg ggcgacgccg cgcataccat
    29281 tcacccactg gcggggcagg gcgttaatct cggatttatg gacgccgcag agctgattgc
    29341 tgaactgaaa cggttgcatc gtcagggtaa agacatcggg cagtacattt acctgcgccg
    29401 ctatgaacgt agccgcaagc acagcgcggc gctgatgctg gctggtatgc agggttttcg
    29461 cgatctcttt tccggcgcta atccggcgaa aaaactgctg cgtgatattg gtttgaaact
    29521 ggccgacacg cttcctggcg ttaagccgca acttatccgc caggcaatgg gattaaacga
    29581 tttgcctgaa tggctgcgtt aaaaatttct cctctgttgt ttatttgata cccatcacac
    29641 tttcatctcc cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc
    29701 tccgttttcg cattatattt tctaatacca tgattttttg atttag
//
LOCUS       DAZSIO010000034        29244 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZSIO010000034 DAZSIO010000000
VERSION     DAZSIO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29244
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.034"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="ACHX5T_001374"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="ACHX5T_001374"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR8466635.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="ACHX5T_001375"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="ACHX5T_001375"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR8466636.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACHX5T_001376"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACHX5T_001376"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR8466637.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACHX5T_001377"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACHX5T_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR8466638.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACHX5T_001378"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACHX5T_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR8466639.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACHX5T_001379"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACHX5T_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR8466640.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="ACHX5T_001380"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="ACHX5T_001380"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR8466641.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="ACHX5T_001381"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="ACHX5T_001381"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR8466642.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="ACHX5T_001382"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="ACHX5T_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR8466643.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACHX5T_001383"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACHX5T_001383"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR8466644.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACHX5T_001384"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACHX5T_001384"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR8466645.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="ACHX5T_001385"
     CDS             8212..9075
                     /locus_tag="ACHX5T_001385"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR8466646.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="ACHX5T_001386"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="ACHX5T_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR8466647.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="ACHX5T_001387"
     CDS             10411..11028
                     /locus_tag="ACHX5T_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR8466648.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="ACHX5T_001388"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="ACHX5T_001388"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR8466649.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="ACHX5T_001389"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="ACHX5T_001389"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR8466650.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="ACHX5T_001390"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="ACHX5T_001390"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR8466651.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="ACHX5T_001391"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="ACHX5T_001391"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR8466652.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="ACHX5T_001392"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="ACHX5T_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR8466653.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="ACHX5T_001393"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="ACHX5T_001393"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR8466654.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="ACHX5T_001394"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="ACHX5T_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR8466655.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="ACHX5T_001395"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="ACHX5T_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR8466656.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="ACHX5T_001396"
     CDS             21859..23568
                     /locus_tag="ACHX5T_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR8466657.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="ACHX5T_001397"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="ACHX5T_001397"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR8466658.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="ACHX5T_001398"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="ACHX5T_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="ACHX5T_001399"
     tRNA            27625..27719
                     /locus_tag="ACHX5T_001399"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            27991..29202
                     /locus_tag="ACHX5T_001400"
     CDS             27991..29202
                     /locus_tag="ACHX5T_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8466659.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
BASE COUNT         7228 a         7834 c         7340 g         6842 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    29101 atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg
    29161 tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc
    29221 tgatgttttc ctcaattaag tatt
//
LOCUS       DAZSIO010000035        28914 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZSIO010000035 DAZSIO010000000
VERSION     DAZSIO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.035"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="ACHX5T_001401"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="ACHX5T_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR8466660.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="ACHX5T_001402"
     CDS             complement(2138..2584)
                     /locus_tag="ACHX5T_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR8466661.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="ACHX5T_001403"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="ACHX5T_001403"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR8466662.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="ACHX5T_001404"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="ACHX5T_001404"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR8466663.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="ACHX5T_001405"
     CDS             complement(5347..6009)
                     /locus_tag="ACHX5T_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR8466664.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="ACHX5T_001406"
     CDS             6182..6595
                     /locus_tag="ACHX5T_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR8466665.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="ACHX5T_001407"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="ACHX5T_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR8466666.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="ACHX5T_001408"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="ACHX5T_001408"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFR8466667.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="ACHX5T_001409"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="ACHX5T_001409"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR8466668.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="ACHX5T_001410"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="ACHX5T_001410"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR8466669.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="ACHX5T_001411"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="ACHX5T_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR8466670.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="ACHX5T_001412"
     tRNA            complement(9861..9948)
                     /locus_tag="ACHX5T_001412"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="ACHX5T_001413"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="ACHX5T_001413"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR8466671.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="ACHX5T_001414"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="ACHX5T_001414"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR8466672.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="ACHX5T_001415"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="ACHX5T_001415"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFR8466673.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /gene="hyaD"
                     /locus_tag="ACHX5T_001416"
     CDS             14005..14592
                     /gene="hyaD"
                     /locus_tag="ACHX5T_001416"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR8466674.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="ACHX5T_001417"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="ACHX5T_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR8466675.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="ACHX5T_001418"
     CDS             14984..15841
                     /locus_tag="ACHX5T_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR8466676.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="ACHX5T_001419"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="ACHX5T_001419"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR8466677.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="ACHX5T_001420"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="ACHX5T_001420"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR8466678.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="ACHX5T_001421"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="ACHX5T_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR8466679.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="ACHX5T_001422"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="ACHX5T_001422"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR8466680.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="ACHX5T_001423"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="ACHX5T_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="ACHX5T_001424"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="ACHX5T_001424"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR8466681.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="ACHX5T_001425"
     CDS             complement(22884..24023)
                     /locus_tag="ACHX5T_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR8466682.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="ACHX5T_001426"
     CDS             complement(24069..26165)
                     /locus_tag="ACHX5T_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR8466683.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="ACHX5T_001427"
     CDS             complement(26165..26911)
                     /locus_tag="ACHX5T_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR8466684.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /gene="gfcB"
                     /locus_tag="ACHX5T_001428"
     CDS             complement(26908..27552)
                     /gene="gfcB"
                     /locus_tag="ACHX5T_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR8466685.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="ACHX5T_001429"
     CDS             complement(27659..27964)
                     /locus_tag="ACHX5T_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466686.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="ACHX5T_001430"
     CDS             complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="ACHX5T_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR8466687.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7003 a         7320 c         7600 g         6991 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgtctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaattgtt
    28201 acgaaagcta ttaattattg ttggtaatat agtttcaagt ggtactataa ctggctgtta
    28261 aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg
    28321 gaaaagtaac tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag
    28381 tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc
    28441 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
    28501 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
    28561 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
    28621 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
    28681 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa
    28741 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
    28801 tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtgg tttctgtatt
    28861 aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta ataa
//
LOCUS       DAZSIO010000036        28523 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZSIO010000036 DAZSIO010000000
VERSION     DAZSIO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.036"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..1175
                     /locus_tag="ACHX5T_001431"
     CDS             <1..1175
                     /locus_tag="ACHX5T_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR8466688.1"
                     /translation="ESYKPIWNWEISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPT
                     STRPTYFGDTDGSVGAVLYALFATGHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNE
                     QLDTNVNTIISILNRRDNVLESLQPYLVINKDAVTPCTFLGDQTGDRFSNICGDQFII
                     DLLKRIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKY
                     KIMANHHGITFDDQRKRYIIGPITVSIDEMTNALDPVELAEIINKKHHAIINGKKFKT
                     SRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRERIGETGTVLG
                     LNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            1365..1478
                     /gene="yhiY"
                     /locus_tag="ACHX5T_001432"
     CDS             1365..1478
                     /gene="yhiY"
                     /locus_tag="ACHX5T_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR8466689.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1541..2608
                     /locus_tag="ACHX5T_001433"
     CDS             1541..2608
                     /locus_tag="ACHX5T_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR8466690.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2605..5340
                     /gene="rbbA"
                     /locus_tag="ACHX5T_001434"
     CDS             2605..5340
                     /gene="rbbA"
                     /locus_tag="ACHX5T_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR8466691.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIQARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5340..6464
                     /locus_tag="ACHX5T_001435"
     CDS             5340..6464
                     /locus_tag="ACHX5T_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8466692.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6523..6798
                     /locus_tag="ACHX5T_001436"
     CDS             6523..6798
                     /locus_tag="ACHX5T_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFR8466693.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            6795..7154
                     /locus_tag="ACHX5T_001437"
     CDS             6795..7154
                     /locus_tag="ACHX5T_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR8466694.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(7274..7675)
                     /gene="nikR"
                     /locus_tag="ACHX5T_001438"
     CDS             complement(7274..7675)
                     /gene="nikR"
                     /locus_tag="ACHX5T_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR8466695.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7681..8487)
                     /gene="nikE"
                     /locus_tag="ACHX5T_001439"
     CDS             complement(7681..8487)
                     /gene="nikE"
                     /locus_tag="ACHX5T_001439"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR8466696.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            complement(8484..9248)
                     /gene="nikD"
                     /locus_tag="ACHX5T_001440"
     CDS             complement(8484..9248)
                     /gene="nikD"
                     /locus_tag="ACHX5T_001440"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR8466697.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(9248..10081)
                     /gene="nikC"
                     /locus_tag="ACHX5T_001441"
     CDS             complement(9248..10081)
                     /gene="nikC"
                     /locus_tag="ACHX5T_001441"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR8466698.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(10078..11022)
                     /gene="nikB"
                     /locus_tag="ACHX5T_001442"
     CDS             complement(10078..11022)
                     /gene="nikB"
                     /locus_tag="ACHX5T_001442"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR8466699.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(11022..12596)
                     /gene="nikA"
                     /locus_tag="ACHX5T_001443"
     CDS             complement(11022..12596)
                     /gene="nikA"
                     /locus_tag="ACHX5T_001443"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR8466700.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12707..13294)
                     /gene="acpT"
                     /locus_tag="ACHX5T_001444"
     CDS             complement(12707..13294)
                     /gene="acpT"
                     /locus_tag="ACHX5T_001444"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR8466701.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(13349..14398)
                     /locus_tag="ACHX5T_001445"
     CDS             complement(13349..14398)
                     /locus_tag="ACHX5T_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR8466702.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            14532..15749
                     /locus_tag="ACHX5T_001446"
     CDS             14532..15749
                     /locus_tag="ACHX5T_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8466703.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15753..16310)
                     /gene="dcrB"
                     /locus_tag="ACHX5T_001447"
     CDS             complement(15753..16310)
                     /gene="dcrB"
                     /locus_tag="ACHX5T_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR8466704.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(16383..17048)
                     /locus_tag="ACHX5T_001448"
     CDS             complement(16383..17048)
                     /locus_tag="ACHX5T_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR8466705.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            17269..17514
                     /gene="tusA"
                     /locus_tag="ACHX5T_001449"
     CDS             17269..17514
                     /gene="tusA"
                     /locus_tag="ACHX5T_001449"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR8466706.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(17616..19814)
                     /gene="zntA"
                     /locus_tag="ACHX5T_001450"
     CDS             complement(17616..19814)
                     /gene="zntA"
                     /locus_tag="ACHX5T_001450"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR8466707.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19888..20514)
                     /locus_tag="ACHX5T_001451"
     CDS             complement(19888..20514)
                     /locus_tag="ACHX5T_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR8466708.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            20655..21014
                     /locus_tag="ACHX5T_001452"
     CDS             20655..21014
                     /locus_tag="ACHX5T_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR8466709.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(21017..21286)
                     /locus_tag="ACHX5T_001453"
     CDS             complement(21017..21286)
                     /locus_tag="ACHX5T_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR8466710.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(21276..21872)
                     /gene="rsmD"
                     /locus_tag="ACHX5T_001454"
     CDS             complement(21276..21872)
                     /gene="rsmD"
                     /locus_tag="ACHX5T_001454"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR8466711.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            22022..23518
                     /gene="ftsY"
                     /locus_tag="ACHX5T_001455"
     CDS             22022..23518
                     /gene="ftsY"
                     /locus_tag="ACHX5T_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR8466712.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            23521..24189
                     /gene="ftsE"
                     /locus_tag="ACHX5T_001456"
     CDS             23521..24189
                     /gene="ftsE"
                     /locus_tag="ACHX5T_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR8466713.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            24182..25240
                     /gene="ftsX"
                     /locus_tag="ACHX5T_001457"
     CDS             24182..25240
                     /gene="ftsX"
                     /locus_tag="ACHX5T_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR8466714.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            25485..26339
                     /gene="rpoH"
                     /locus_tag="ACHX5T_001458"
     CDS             25485..26339
                     /gene="rpoH"
                     /locus_tag="ACHX5T_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR8466715.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            26610..27713
                     /gene="livJ"
                     /locus_tag="ACHX5T_001459"
     CDS             26610..27713
                     /gene="livJ"
                     /locus_tag="ACHX5T_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR8466716.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(27912..28295)
                     /gene="panM"
                     /locus_tag="ACHX5T_001460"
     CDS             complement(27912..28295)
                     /gene="panM"
                     /locus_tag="ACHX5T_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR8466717.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6819 a         7347 c         7851 g         6506 t
ORIGIN      
        1 ctgagtcgta caaacccatc tggaactggg aaatatcgca atacaatgta tttgatatta
       61 aattcgaaac aattataaaa ccccagttcg ctgacagaac ctatttctcc aattgttcac
      121 ctgttgatcc cacaagtacc aggccgacct attttggcga taccgatggc tcagtcggtg
      181 cagttttgta cgcgcttttt gcaacaggac atttgggaat catggctgaa ggagaaaatt
      241 tccttagcca gttgcttaat atagaagatg aagttctcaa cgtattgcta agggagaact
      301 ttaatgagca acttgacact aatgttaata caataatcag tatactgaac agaagagata
      361 atgttttaga aagtcttcag ccttatttgg ttattaataa agacgcagtc acgccatgta
      421 cgtttcttgg cgaccaaacc ggcgatcgat ttagtaatat ctgtggcgat caatttatca
      481 tcgatttgtt aaaacgcata atgagtatca atgaaaatgt tcatgtatta gctggaaatc
      541 atgaaacgaa ttgcaatggt aactatatgc aaaacttcac gcgcatgaaa ccacttgatg
      601 aggataccta cgacgggata aaagattatc ctgtatgttt ctatgacccc aaatacaaaa
      661 taatggcaaa tcatcatggg ataacttttg atgatcagcg aaaacgttac attataggac
      721 cgataactgt ctctattgat gaaatgacca atgctctcga tccggtcgaa ttagcggaaa
      781 ttattaataa aaagcatcat gctataatta atggcaaaaa gtttaaaact agccgggcta
      841 tctcttgtcg atcctttaat cgttattttt ctgtttcgac agattacaga cctaaacttg
      901 aagcactatt agcatgttca caaatgttag gtataaatca agttgttgca cataacggaa
      961 atggtgggag agaacgcata ggtgaaacgg ggacagtttt aggtcttaac gcccgtgaca
     1021 gtaaacatgc tggaagaatg ttcagcatgc ataattgcca aattaaccct ggtgcagggc
     1081 cagagataac cactccctgg aagtcttacc agcatgaaaa aaacagaaat ggacttatgc
     1141 ctttgattag aaggcgcaca atgttacagc tctgaaccat agacaacatg aatattaatg
     1201 ttactatctg gttgagactg ttgataaaac gtaaaaagga atgctctgtt cctgcaactg
     1261 aacaggcgtg aaatgcgatc caacaggatg gataccgttt tgtcagccag ttttatagat
     1321 cacacttatg agtatgcgtg tttcatttac ggccaggaat gctgatgatg acaacattac
     1381 tacccgtttt cactaagcct tccccgttgg cgctcaatgc tctgcgcgct ggtcgtattt
     1441 gtcgtttcct tcttatcccg gatggaagaa tccgctgaac gtcgttcggc ggcagttgta
     1501 acacgcgtcg tttgcttaca gggattatgg cgggtcgatt atggataaga gtaagcgcca
     1561 tctggcgtgg tgggttgtcg gggcactggc ggtggctgcc gtcgtggcgt ggtggctgtt
     1621 gcgcccggcg ggtgtgccgg aaggctttgc tgtcagcaat gggcgcattg aagcgacgga
     1681 agtggatatc gccagcaaaa ttgccgggcg tatcgacacc attctggtga aagaaggcca
     1741 gtttgttcgc gaaggcgaag tgctggcgaa gatggatact cgcgtgttgc aggaacagcg
     1801 actggaagcc atcgcgcaaa tcaaagaggc acaaagcgcc gttgccgccg cgcaggcttt
     1861 gctggagcaa cgacaaagtg aaactcgtgc cgcacagtcg ctggtcaatc aacgccaggc
     1921 cgaactggac tctgttgcca aacgtcatac gcgttcccgc tcgctcgccc aacgaggggc
     1981 tatttctgcg caacagctgg atgacgaccg cgccgccgct gagagcgccc gagctgcgct
     2041 ggaatcagcg aaagcccagg tttcggcttc taaagcggct atagaagcag cacgcaccaa
     2101 tatcattcag gcgcaaactc gggtcgaagc agcgcaagcc actgaacggc gcattgccgc
     2161 agatatcgat gacagtgaac tgaaagcccc gcgtgacgga cgcgtgcagt atcgggttgc
     2221 cgagccaggg gaagtgctgg cggcaggcgg tcgggtgctg aatatggtcg atctcagcga
     2281 cgtctatatg accttcttcc tgccaaccga acaggcgggc acgctgaaac tgggcggtga
     2341 agcccggctg atcctcgatg ccgcgccaga tctgcgtatt cctgcaacca tcagttttgt
     2401 cgccagtgtc gcccagttca cgccaaaaac cgtcgaaacc agcgatgaac ggctgaaact
     2461 gatgttccgc gtcaaagcgc gtatcccacc ggaattactc cagcagcatc tggaatatgt
     2521 caaaaccggt ttaccgggcg tagcgtgggt gcgggtgaat gaagaacttc cgtggcctga
     2581 cgacctcgtg gtgaggttgc cgcaatgacg catctggaac tggttcccgt cccgcctgtc
     2641 gcgcaactgg cgggcgtgag ccagcattat ggaaaaaccg ttgcgctgaa caatatcact
     2701 ctcgatattc aggcccgttg catggtcggg ctgattggtc cggatggcgt cgggaagtcg
     2761 agtttattgt cactgatttc cggtgcccgc gtcattgagc agggcaatgt ggtggtgctg
     2821 ggtggcgata tgcacgaccc gaagcatcgc cgagacgtct gcccgcgcat cgcctggatg
     2881 ccgcaggggc tgggcaaaaa cctctaccac accttgtcgg tgtatgaaaa tgtcgatttt
     2941 ttcgctcgcc tgttcggtca cgataaagcg gagcgggaag cgcgaattaa tgagctgctg
     3001 accagcaccg ggttagcacc gtttcgcgat cgtccggcag gtaaactctc cggcgggatg
     3061 aagcaaaaac ttgggctgtg ctgcgcgtta atccacgacc cggaactgtt gatccttgat
     3121 gagccaacaa cgggggttga cccgctctcc cgcgcccagt tctgggatct gatcgacagt
     3181 attcgccagc ggcagagcaa tatgagcgtg ctggtcgcca ccgcctatat ggaagaggcc
     3241 gaacgcttcg actggctggt agcgatgaat gccggagaag tgctggcaac cggcagcgcc
     3301 gaagagctgc ggcagcaaac gcaaagcgcc acgctggaag aagcatttat taatctgtta
     3361 ccgcaagcgc aacgccaggc gcatcaggcg gtagtgatcc caccgtatca acctgaaaac
     3421 gcagagattg ccatcgaagc gcgcgatctg accatgcgtt ttggttcctt cgttgccgtt
     3481 gatcacgtca atttccgcat tccacgcggg gagatttttg gattccttgg ttcgaacggc
     3541 tgcggtaaat ccaccaccat gaaaatgctt accggactgc tgcccgccag cgaaggtgag
     3601 gcgtggctat ttgggcaacc ggttgatcca aaagatatcg atacccgccg tcgtgtgggc
     3661 tatatgtcgc aggcgttttc gctctataac gaactcaccg tgcggcaaaa cctggagtta
     3721 catgcccgtt tgtttcacat cccggaagcg gaaattcccg cgcgagtggc tgaaatgagc
     3781 gagcgtttta agctcaacga cgttgaagat gttctgccgg agtcattgcc gctcggcatt
     3841 cgccagcggc tttcgctggc ggtggcggtg attcatcgcc cggagatgtt aatcctcgat
     3901 gagcctactt ctggtgtcga tccggtggcg agggatatgt tctggcagtt gatggtcgat
     3961 ctctcgcgcc aggacaaagt gactatcttc atctccaccc actttatgaa cgaagcggaa
     4021 cgttgcgacc gcatctcact gatgcacgcc ggaaaagtgc ttgccagcgg tacaccgcag
     4081 gaactggttg agaaacgcgg agccgccagt ctggaagagg catttatcgc ctatttgcag
     4141 gaagcggcag ggcagagcaa cgaagccgaa gcgccgcccg tggtacacga caccacccac
     4201 gcgccgcgtc agggatttag cctgcgccgt ctgtttagct acagccgccg cgaagcgctg
     4261 gaactgcgac gcgatccagt acgttcgacg ctggcgctga tgggaacggt gatcctgatg
     4321 ctgataatgg gttacggcat cagtatggat gtggaaaacc tgcgctttgc ggtgctcgac
     4381 cgcgaccaga ccgtcagtag ccaggcgtgg acactcaacc tctccggttc ccgttacttt
     4441 atcgaacagc cgccgctcac cagttatgac gagcttgatc gtcggatgcg tgcgggcgat
     4501 atcacggtgg ccattgagat cccgcccaat ttcgggcgcg atatcgcgcg tggtacgcct
     4561 gtggaactcg gcgtctggat cgacggagcg atgcccagtc gtgccgaaac ggtaaaaggt
     4621 tacgtgcagg caatgcacca gagttggctt caggatgtgg cgagccgaca atcgacaccc
     4681 gccagccaaa gcgggctgat gaacattgag acgcgttatc gctataaccc ggacgtaaaa
     4741 agcctgccag cgattgttcc ggcggtgatc ccgcttctgc tgatgatgat cccgtcaatg
     4801 ctaagcgccc ttagcgtggt gcgggaaaaa gagctagggt cgattatcaa cctttacgtg
     4861 acccccacca cccgtagcga atttttgctt ggcaaacagc tgccgtacat cgcgctgggg
     4921 atgctgaact ttttcctgct ctgcggcctg tcggtgtttg tgtttggcgt accgcataaa
     4981 ggcagtttcc tgacgctcac cttggcggcg ctgctgtata tcatcattgc cactggaatg
     5041 gggctgctga tctccacctt tatgaaaagc cagattgccg ccattttcgg aacggcgatt
     5101 atcacattga tcccggcgac acagttttcc gggatgatcg atccggtagc ttcgctggaa
     5161 gggcctggac gttggatcgg cgaggtttac ccgaccagtc attttctgac tatcgcccgc
     5221 ggaacgttct cgaaagcgct ggatctgact gatttgtggc aactttttat cccgttgctg
     5281 atagccatcc cgctggtgat gggcttaagc atcctgctgc tgaaaaaaca ggagggatga
     5341 tgcgccattt acgcaatatt tttaatctgg gtatcaaaga gttgcgcagt ctgctcggtg
     5401 ataaagcgat gctgacgctg attgtcttct cgtttacggt gtcggtgtat tcgtcagcga
     5461 ccgttacgcc aggatcgttg aacctcgcgc cgatcgccat tgccgatatg gatcaatcgc
     5521 agttatcgaa ccggatcgtt aacagcttct atcgtccgtg gtttttgcca ccggagatga
     5581 tcaccgccga tgagatggat gccggactgg acgccggacg ctataccttc gcgataaata
     5641 ttccgcctaa ttttcagcgt gatgtcctcg ccggacgcca gccggatatt caggtgaacg
     5701 tcgatgccac gcgcatgagc caggcattta ccggcaatgg gtatatccag aatattatca
     5761 acggtgaagt gaacagcttt gtcgcgcgct accgtgataa cagcgaaccg ttggtatcgc
     5821 tggaaacccg gatgcgcttt aacccgaacc ttgatcccgc gtggtttggc ggggtgatgg
     5881 cgatcatcaa caacattacc atgctggcga ttgtattgac cggatcggcg ctgatccgcg
     5941 agcgtgaaca cggcacggtg gaacatttac tggtgatgcc gataacgccg tttgagatca
     6001 tgatggcgaa gatctggtcg atggggctgg tggtgctggt ggtatcggga ttatcgctgg
     6061 tgctgatggt gaaaggtgta ctgggcgtac cgattgaagg ctcgatcccg ctgtttatgc
     6121 tgggcgtggc gctcagtctg tttgccacca cgtcaatcgg catttttatg gggacgatag
     6181 cgcgttcaat gccgcaactg gggctgctgg tgattctggt gctgctgccg ctgcaaatgc
     6241 tttccggtgg atccacgccg cgcgaaagta tgccgcagat ggtgcaggac attatgctga
     6301 ccatgccgac gacacacttt gttagcctcg cgcaggccat cctctaccgg ggtgccggat
     6361 tcgaaatcgt ctggccgcag ttcctgacgc tgatggcaat tggcggcgcg tttttcacca
     6421 tcgcactgct gcgattcagg aagacgattg ggacaatggc gtaaggaagg gaaacttgac
     6481 ttcatactgt gatgtcataa agtgaagtca taaggacatg ccatgagaac caaggtacag
     6541 gctttacgga agaaacaaaa aaatacattg gatcaaatat ttaaaactcc tgttcctcaa
     6601 gggatcaagt ggtctgatat agagtcactg gttaaagcat taggcggaga aattaaggaa
     6661 ggaagaggtt cgcgttgcaa gttcatacta aatatgagcg ttgcgtgttt ccatcggcct
     6721 catccgtcgc cagataccga taaaggcgct gtagaaagcg tgtgtgactg gttactaagt
     6781 ataggggtaa aaccatgatc aaactgaaga cgccaaactc tatggagatt gccgggcaac
     6841 cagctgttat tacctacgtg ccagaactga atgcatttcg tggcaaattc ctgggcttgt
     6901 caggttattg tgattttgtc tctgacagta ttcaggggct gcaaaaagaa ggagagcttt
     6961 ccctgcgaga gtatcttgag gattgtaaag ctgcgggtat tgagccgtat gcgcgtacag
     7021 aaaagattaa gacctttacg ttacgctatc cagaatcgct gagtgaacgc cttaataatg
     7081 cggctgcgca gcaacaagtc tctgttaata cctatattat tgaaacactg aatgagcgtt
     7141 tgaatcattt ataaatttta gcgacgtaca ttacgcagaa tatctgcctt tacaggatct
     7201 atctatgcaa ctgaacttaa ttttataaat taagttcagt taatatcttt tctttccttg
     7261 ttttagaaat aactcaatct tccttcggca ggcactgcaa atgcccgtgc cgcacgccgc
     7321 gctgggcgat aacgtcatcg gcaaaatgct gcacgtcacc catgtcacct ttcaacacgg
     7381 cgatttccag acagtcgtcg tggttgatgt gcacatgtag cgtggcgacg gagaggtcgt
     7441 ggtgatgatg ctgggtggaa acaatacggc tggctaagtc gcgtttttcg tgttcataca
     7501 catacgacag caccgcgaaa ccttgtgtgc cgtgctgctg ggtagcttcc tgcgccaggg
     7561 cactacgcag aatatcgcgg atagcttcgg aacggttgtt ataaccacga cgctggctca
     7621 ggctgtccag cgtctccagt aaatcgtcat caagcgtgat ggtgactcgt tgcattggcg
     7681 ttaaaccttt tctgaggtgc gacggcgcac ggggaatgcg ggtaataccg cgttttgtag
     7741 cacacgtccg gcgtcagagg aaaaggttaa tttatctccc accgcctggg tttcggcgat
     7801 ttgtccgttg tccatcacca tcactcgctg gcaaaagcgt tccaccaggc gtaagtcatg
     7861 agtgatgaac aggcaggcag tgccaaactg ttgttgtagc tttttcagca ggcgaatgac
     7921 gcccgcctgt aacacgagat caaggttaga aacggcttca tccagaatca gcaatttcgg
     7981 ttcgaccgcc agcgcgcgtg ccaggcagac gagctggagc tggccgccgc ttaactgcgg
     8041 tgggcgtttg tcgagaacgc tgtcatcgag atcgaccgcc ttcagcattt cgctggcgcg
     8101 cgccagttgt tcggattttt tcagtgatag caggtggcgc atcggttcac gcaggatctc
     8161 gcgcacggtt ttgcgcggat tcacggcact gatggagtcc tgaaacacca tctgaatatc
     8221 acggcggaag gctttacgtt gggcgcgatt aagtttcgcc agcgattcgc cgtgccagct
     8281 aatattccct tgtgagggag actctaagcc taccagcagc cgcgcgaggg tgctttttcc
     8341 gcagccgctg cgtcccagca gggcgacggt ttcgccgctt ttcagggtca gggaaacgtt
     8401 attcagcacc gcctgatgtt gatgttttcc gctaaatcca ccgtgggcat agtgatggga
     8461 aaggccgcag acgttaagta aggtcatgat gccagctcca taccgtagag ggcgagatga
     8521 gcggaaacca ggctgcgcgt caccgtatgt ttgggggcgt taaacagcgt ttctacatcg
     8581 ccctgttcga caattttacc ttgtgacatc accgccacgt catccgccag acgcgccaca
     8641 acgcccatat catgggtgac cagcagcatt cccggcgctt gtttttgcat aatgctttcc
     8701 agcagatcga ggatgcgcgc ctgtgctacc acgtcgaggt cggtggtcgg ttcatcggcg
     8761 atgataaacg gtgattcaca cagcaccgcc atcgcaatca tcatgcgctg caacatgccg
     8821 ccgctcatct cgaacgggta cagcttcagc acgcgcgcgg cgttttccag ccccaccgct
     8881 tctatggcag cggtaagcgt agcgtcatcg gcgggtttcc ctaacgccag gcaggtttcc
     8941 cgcgcgtggg tgtgcatggt gtgcagtgga ttaaaggcgc tgcgcgggtt ctgcatgatg
     9001 gtggcaattt tgatgccgcg cagggcgtaa ggcgaaaccg gtttgccatc ggctaaaatt
     9061 tcccccgccg tctggcgaac gccagcgggc aaaatgccca gcgtcgcggc gcaggttaat
     9121 gatttcccgc tgccgctacc gccgactaac gccagcacgc gcccgcgttg cagggttaac
     9181 gatacaccgt gtaccagcgg ctgcgcggcc tgtagcgtga tattacgtag ttcaatctgt
     9241 tgcggcatta gtgtgcgtgc tccgtcacca gatgaggatc cagatgatcg cgcagtgcgt
     9301 cacccaccag gttaaaggcc atcacgctga taaacagcgc cagccccggc cagaacattt
     9361 gcagcggctg ggtccagata tactggcgcg cgtcgttaat catcacgccc cattcggcgg
     9421 tcggcgcggt cacaccgagg ccaaggaaag acatccccgc gacgtgcagc atcatatggc
     9481 cgatatccag cgttgccagc accagcagcg aagggatcac cgcgcctgcc agatgatcga
     9541 caaacacccg cacatggccc gcaccggaaa gccgtgacgc cagcacaaac tcgcgttggc
     9601 gtagtgaaat caccaagctg cgcaccatgc gtgcatacca cgcccagtgc gacagggcga
     9661 tggcgataat tacgttggtc agcccggtgc cgagcacgcc aaccataaag aacgacagaa
     9721 tcgaggtcgg gaaggtcata aacatatcgg cgacgcgcat ggtggcctga tcaacgtgcc
     9781 cgccaatcaa cccggcgctg ccgccaataa ccagccctaa tgtcagcacc agcagcaggc
     9841 aggccattac cgaaccgagc gacacgcggg tcgctgccat cagccgcgag aaaatatcgc
     9901 gacctaagtg atcggtgccc agccagtgct gcgcatccgg cgaaagcagg cacgacggca
     9961 aatcaattgc ctgtggatcg tacggctgcc accactggct ggtgagcgca atcagcgcca
    10021 gcagggcgat aatgatcagc gccaggcgta ccgaccagcg ggaagagagg aaaaagttca
    10081 cgcgtgcgct ccttcatgac gacgaatgcg cgggtccagc gcggcgttga gcaaatcgac
    10141 aatcaaatta cagaccacaa aaaccaccac catcatcagc gtaaagcact gaatcaccgg
    10201 atagtcacgg ttaaaaatcg ccgacaccgc atagcgcccg acgccaggcc aggcaaagat
    10261 gttttcgata atcatcgtcc cgccaatcaa ttcgccaatg tgcatcccca cggcggtgat
    10321 catcggcagc gaggcattgc gcagaatatg gcggcgttcg gtctgtttgt cattcaggcc
    10381 gcgtagccgc gcccaggtga cgtgacgctg accggcgacg tccagcatac tggcgcgcag
    10441 cagtcgcgcg ttaatcgcca gcgacataaa ggcgatggaa accgcaggta aaatgatgtg
    10501 ctgccagctg ccgtagccca tcgcgggtag ccattgcaga tacaccgaaa acgccatcac
    10561 tagcaaaaat gccagccaga agttgggcat cgacacgcca agaaacgcga taaagcgcac
    10621 ggcgaaatcc ggcaaacgat cgcggtggcg cgccgcccag ataccgagcg gtacggaagt
    10681 gagcagaatt aataccagcg ccgcacctgc aagttccagc gtggcgggca ggaagttcag
    10741 catatcgtcc agtaccgggc gttggctggc gaatgagata ccaaagtcaa gatgcagcgc
    10801 cttccacaac caggtaccgt actggacgta cagcggctga tccagcccga gcatggtgcg
    10861 ggtagaggcc agcatctccg gcgtcggcgg caggttagat aaacgcagat aatcgagcgc
    10921 cgggtcgccg gtaccgaggc gcagcatcag aaaaatgatc accgaggcgg cgagcaccat
    10981 cgggatcagc agcagaaagc ggcgtaatac gtaacgcaac attaaggttt caccggttta
    11041 atctgttcga acggaatttc ggtggcgatc ggcgcgtagg ggatattacc cagctccggt
    11101 tttgatacca ccatcatcga gatgtagctg ataggcagat aaaccgcctc gtcatgcaga
    11161 cgggtcagaa tgtcgcgata cagcgcctga cgttgcgttt cgtcatgggt cgccagcact
    11221 tcgccgatct ctttatcaat cagcggcttg tcggctaatc cttgctgtgc ctggaagtca
    11281 gcgtgcgacg gtacgcgcat tgaactcagg aaggcgtgtg gatcgtatgg cgcgccccag
    11341 gtgcggtgga aaatcatgcc aaaacgaccg tcgcgctgac gagcatagat actgctctct
    11401 tcttcgccaa tcagcgagac atctgcgcca atctggcgca tatcagcctg aatgatttcc
    11461 gccatcgatt tgcttaacgc atcggtgccg atgaacgaca gttcaatgcg cagcggctga
    11521 ccatttttct cgcggatgtc tttgcccgca ggcagcgtcc aaccggcttt ttccagcaac
    11581 tctttcgctt tctgcggatc gtactggcgc ggtttcaggc cgagattagc gtaaggcaca
    11641 gaaggggcaa acagggtgtc ggcgacctgc tgggtgccat ataacgcgtt atcaatcagc
    11701 gattttttgt ttaccgcgta attaagagct tcacgtactg ccagctcgtt ggtgggggct
    11761 ttggcggtat tgagcgccag catcacggtt tcgatcggct gtgacagttg ggtgtggtaa
    11821 gccggattct ggctaaagcg ggcgaaggta tcgagcggta ataacccttc gtttccgtac
    11881 agcaggtcga tatcgccagt ttcaaacgcc accgcgcggg tagtcgggtc cgggatgacg
    11941 ttaaaggtga tctttttaat cgctggcttt tcgccccagt agttttcgtt acggacgaag
    12001 acatcgtact gatttagttt cgattcctgc aaaatccacg gtccggtgcc aatcggcgct
    12061 ttaattccgt tcatggtttc atggttttta aactgcgagg gagcgataaa acggaaagga
    12121 cggggcaggg ccagttcttg caggaaagga tagtaggcgc ttttcagggt aatttgcagc
    12181 tctgttttat tgagtgcttt aacatcaaca atctggtttg ccagctccag ccaggcgtga
    12241 cgttgacggt tatcgagcac tgcgcggaag ttttctgccg ccgcctcggc atcgaacggt
    12301 tcaccgttgg agaatttcac gtcatcacgc agggtgaagg tccaggtttt accatcttct
    12361 gaatgagtcc agctttttgc cagccacggg atcaccgaac cgtctgcctg atatttcacc
    12421 aatggttcat aaaccatgct ctgggcgaac atctggttag gcgtgtaaag gtgcgggttt
    12481 agtggcccga cattcaccgg ccaggcggtg gtgatttcat ctggtgcagc ggcatggacg
    12541 ataaaagacg cacaagccag cagcgcaaat agagtgcggc ggagtgtgga gagcatggta
    12601 accccaatgg attaaaatag atggcggaaa taagtatgac gattttaagt attcgtcata
    12661 ctgattacct gttctggatc aatagatggg cagtcgggtg ggcggatcag ttaactgaat
    12721 cgatccattg cacactgtcg gcggtgagcg taaagggggt gggggtgcag atcgcaaggc
    12781 ttaaattttc tagctgacaa tggctgactg acagcgagga gtgataggtg ctatctacgc
    12841 tgacgatttg ccaggcgctg ccgccacgct gtttaacgat ggcttctttg cgcgtccaga
    12901 tgcgccagaa catttccagt tgctgatcag gatgcacggc gtccatttca gcgtgttccc
    12961 cgaggctgaa cacggcgttc gccagccagc gccagttgac gcgcgggcga atcacttcga
    13021 tatcgcagcc gacttcgcct tcatcgctca acagcagagc gatatcgtca ccgctatggc
    13081 ttaagttgaa ccatagcggc gtttccggcg cgaatgcagg tttgccttgt tcgccataga
    13141 tgatctccgg tagcggggaa agcgtgtgcg aaagcaatgt acgccccgcc agccagcgtt
    13201 cgcgtcgtgg accttgcggt gcttgctcgc gtaaacccgg tggcagtgga gctgcgctta
    13261 aggtcgaaac tttccccaga actatccgat acatatcagg gccaacgttt aatggaaaat
    13321 gaaagtgcgt atcgtatcac ttgtcgcctc atcccggtaa ccgacttttc ggtctgcccg
    13381 gccccagtaa aatcgccagt ttgctgccac ctttcgtcgt ttccatccag attttacaca
    13441 cgctggttaa tggcaccgaa agtagcatcc ctaccgggcc gagcagccat ccccagatta
    13501 acaatgaaag aaataccacc atggtggaca tccccaggcg atggcccatc atccgtggtt
    13561 ctaaaatatt gccgatgacc atatggacca ctaaaaacaa tgcgccgacc agaatacatt
    13621 cgtaaacacc attaaacagc agcacctgaa tcattggcgg tacggcggaa attaccgcgc
    13681 cgatattggg cacgtagttg agcaaaaacg ccagtactgc ccacatcagc gcaaactgta
    13741 cgcccattag cgccagcccc agccagacga tgacacctgt ccataaactg agtagcgtct
    13801 ttaacgccag ataatgcgaa acgcctttaa gtgcgcggtg taatcctgcg atgtgaatct
    13861 gtgggttatt cagcgcaaaa cgcattttgt aagggacgtg gcgcacttca aacagcataa
    13921 aaactacggt catcaccagc aaaagcacgc tcgccattgc cccggaaagc ccggtcatta
    13981 gcgctgtggt gaaggtaacc actttttccg agtccatccg ctgcagcatt cgctccggcg
    14041 acatatgcaa attaagaaaa ggcaacatct cctgcaattt aaaaagtttg cgcgtcagct
    14101 ccttattaaa cttcggcagc atagagataa attcgttaaa cgatgccgcc agtacgccga
    14161 ccagcgcggt tagtgcgatc agcatcacca ccactacaat cgtaatggca acggggcgtt
    14221 gtactccccg acgaataaac caggtaacga gcgggttgag gacgatggca aaaaacagcg
    14281 ccagtaacag ctgcacaatg atatctgccg ctgcgtgaat gcccgcgagg atcactacca
    14341 gcgaggccaa cttgagcaga atgtgcatgc ccgttttatc gggttgaggg gtttccattg
    14401 gggcttcctt gtgacttttt gtgttaagtg tagcgcggga gtcacgccag caatattctg
    14461 attcttactg ctgattttca cgtcagcaca tggtaaaaat gaaacactgt tgtaaaaatg
    14521 tggtgatcct catgcccgaa cccgtagccg aacccgcgct aaacggattg cgcctgaatt
    14581 tgcgcattgt ctccattgtc atgtttaact tcgccagcta cctcaccatc gggttgccgc
    14641 tcgctgtatt accgggctat gtccatgatg tgatgggctt tagcgccttc tgggcaggat
    14701 tggttatcag cctgcaatat ttcgccacct tgctgagccg ccctcatgcc ggacgttacg
    14761 ccgatttgct gggacccaaa aagattgtcg tcttcggttt atgcggctgc tttttgagcg
    14821 gtctggggta tctgacggca ggattaaccg ccagtctgcc tgtcatcagc ctgttattac
    14881 tttgcctggg gcgcgtcatc cttgggattg ggcaaagttt tgccggaacg ggatcgaccc
    14941 tatggggcgt tggagtggtt ggctcgctgc atatcgggcg ggtgatttcg tggaacggca
    15001 ttgtcactta cggggcgatg gcgatgggtg cgccgttagg cgtcgtgttt tatcactggg
    15061 gcggcttgca ggcgttagcg ttaatcatta tgggcgtggc gctggtggcc attttgttgg
    15121 cgatcccgcg tccgacggta aaagccagta aaggcaaacc gctgccgttt cgcgcggtgc
    15181 ttgggcgcgt ctggctgtac ggtatggcgc tggcactggc ttccgccgga tttggcgtca
    15241 tcgccacctt tatcacgctg ttttatgacg ctaaaggttg ggacggtgcg gctttcgcgc
    15301 tgacgctgtt tagctgtgcg tttgtcggta cgcgtttgtt attccctaac ggcattaacc
    15361 gtatcggtgg cttaaacgta gcgatgattt gctttagcgt tgagataatc ggcctgctac
    15421 tggttggcgt ggcgactatg ccgtggatgg cgaaaatcgg cgtcttactg gcgggggccg
    15481 ggttttcgct ggtgttcccg gcattgggtg tagtggcggt aaaagcggtt ccgcagcaaa
    15541 atcagggggc ggcgctggca acttacaccg tatttatgga tttatcgctt ggcgtgactg
    15601 gaccactggc tgggctggtg atgagctggg cgggcgtacc ggtgatttat ctggcggcgg
    15661 cgggactggt cgcaatcgcg ttattactga cgtggcgatt aaaaaaacgg cctccggaac
    15721 acgtccctga ggccgcctca tcatcttaaa acttactgaa taaccagcgt attaatgatg
    15781 ttttctgcgg tggtttgcgc tttttgctga tcgtcagctg gcagcgtaat ttgcatggtc
    15841 agcagttgat tacccacgtt acccagaata acggaagagt acgccgtctg gcctttcgcg
    15901 gagataatac tgtctaactg ctgcattttg tgacctttca gctcaatggc tttattggtt
    15961 accacttgca gctgcggatc gcggctacgt tgctgatctt ccagacgctt cgccaacacc
    16021 gccagatctt ctttcggatc atcgcccatg atgacgatga ctgctttctg cccggtggcg
    16081 tcggaccaga catgcatgtt attggcctgc gttcccagct taccgctctg gtcggtcata
    16141 tccgctggca gcgagaaact taacttgcca tcaagcaggt tgacgggatt cccggtagcg
    16201 ttactttccg caaccgaacc ctgcgccgta gcgttagtgt ctttatcatc acaggccgca
    16261 agccccataa ccagcaggcc aattccgaca tatttaacca gattgcgcat tgacttcttc
    16321 ctttcgataa acggccataa cggctcattc atccatctta tcacaactct gataacgaac
    16381 ctttaactcg cctgcaaagc gttgatttcg gatttatctg ccagtctttt caacagcata
    16441 ttgagtaata cgccatacat tggcaggaag aaaacgatac tgattaacac tttgaaacag
    16501 taatcgacca gcgcgatttc catccagtgt tcagccataa aggcatccgg gctacgccag
    16561 aaggcaatga agaaaaaggc cagcgtgtcg ctgacgttac cgaacagtgt ggacgctgtc
    16621 ggtgccagcc accagcggcg actctgacgc aggcggttaa aaacgtgcac gtcgaggatt
    16681 tgccccagcg cgtaggccat gaaactggcg gtggcgatac gggcgacaaa caggttgaag
    16741 tgggcgagtg cgccgaatcc ctgccaggaa cccatataga atagcgacga gatgacgtag
    16801 gagattaata acgcagggat cattaccgcg aagataatgc gtcgggccag cggtgcgcca
    16861 aaaatacgca cggtcaggtc ggtagcaaga aaaataaacg gaaagctaaa cgcgccccag
    16921 gtggtatgga aaccaaaaat ggagacggga agctgaacca gatagttact ggaggtgatc
    16981 accagaagat gaaataacga taaccagaac aacgccttat agcgttgagt ttgcgagaaa
    17041 acgttcatat tgtacctttt tgattaacca ttggggtgag ggaacccaat acgtacgaca
    17101 cgtctgttac cttaacataa taacgtgctt accctttttt cgagccgccg catgatactg
    17161 ctttacgttg acaatgcaat ggttaatttt cacgcaatcg ttaacctggt ttgcttacgg
    17221 actcacaggg cgtaaactag cgccgttttt ttaagtgatg agaagaaaat gaccgatctc
    17281 ttttccagcc ctgaccacac actcgacgcg cttggcctgc gctgcccgga accggtgatg
    17341 atggtgcgca aaaccgtgcg caatatgcag cctggcgaaa cgttgctgat tatcgccgac
    17401 gatccggcca ctacccgcga tattcctggg ttttgtacct ttatggaaca cgaactggtt
    17461 gcgaaagaga cggatggact gccttatcgt tatttgattc gtaaaggcgg ttgatagggg
    17521 ctgattggct tcgatgccgc cttttcctca ccccctaacc ctctccccag aggggcgagg
    17581 ggaccgatcg cgctcaatgt tgcgatcggt ttgccttatc tcctgcgcaa caaccttaac
    17641 gcattcgccg tcaccagcac cgtcgcaccc gtatctgcca gcactgccag ccacaacccg
    17701 gtcatcccta aaagcgtggt gacgaggaag atccctttca gtcccagcgc aatggtgatg
    17761 ttctggcgga tattggcgtg agtggcgcgt gccagttcaa tcatttgcac caggccgcgc
    17821 aggtggttat gggttaatgc tgcgtcggcg gtttccagcg ccacgtctgt gccgctaccc
    17881 atagcaatcc cgatggcggc agctttcatc gctggcgcgt cgttaatacc atcaccgacc
    17941 atcgccaggg gcgcatgttg attcagcttg gtcaccgctt tgactttatc ttccggcaat
    18001 aggcccgctt taaactccag ccccagctcc ccggcaattg ccgccgctgc gcgtggatta
    18061 tcgccggtga ggatcactcc tttaaccccc agcgcgttca gttcactgat ggcagttgta
    18121 gcgtcggcgc gcagggtatc ctgcaatgca atgataccca gcacgtcatc attacgtact
    18181 accagcacaa ccgtttgccc ggcgctttcc agttcattaa tcaaaccagc aaatgcatca
    18241 gcgggatgtt tcccggcagc gcatatcaac acgcgctcac cattaacctg cgcttcaatg
    18301 ccagacccga ccagtgcccg ctgtgattcg gcggtgggaa tggcgagttc agcaacctgt
    18361 gcttcgcgta cgatggcttg cgccagtgga tgcgtcgcgc cttgctcgac cgccgccgcc
    18421 agtgtcagca gttcagattc actaataccc gttgccggat gaatcgcggt aacgcgcggt
    18481 ttaccgacgg tcagcgtacc ggttttatca aacgccactt gggtaacacg acctagttgt
    18541 tccagcgccg caccgccttt aattaacgcc ccacgacgcg ctgctgctgc cagcccggag
    18601 gttatcgccg caggcgttga gataactaac gcacacgggc aaccaatcag cagcagcgtc
    18661 agacctttat aaatccactc ctgccagctg gcggcaaaca gcagcggcgg cactagcgtt
    18721 accagcaggg cgacggccat aatcgctggc gtataaatac ggctgaaacg gtcgataaac
    18781 cgctcaatgg gggcgcggcg ctcttcggct tcttcgatca gtttcagaat ccggtcaatg
    18841 gcgctggcac ccggttctga cagcacttcc agcgttacca gacggtctac gctggtggct
    18901 cctgcaggaa ctttatcgcc cgtcgcgcgc tccaccggaa tggattctcc ggttagggcg
    18961 ctttcatcaa aactggcaaa cggtgagagc agtttaccgt cggcaggcaa acgcccacct
    19021 gcggcgactt caatcacatc gccaggccgc aggctgttaa tcgccacctc ttcccgctca
    19081 ccgttacgca ggcgcgtggc ggtttctggt ttcagcgcca ttaacgcgct aacgccctga
    19141 cgcgcgcggc tggcggccca gccttccagt cgttcaccaa tcaaaaacag cagcaacacc
    19201 atcgcagctt cagccgttgc gccaataaac agtgcaccaa tagcggctac gctcattaag
    19261 gtttcaatgg cgaagtagct gccggatttg atcagccgta atgcctgacg agcaatcggg
    19321 tacagcccaa ctagcgtggt cgcgataaac gccagttgcc cgaacggatg attgaactgc
    19381 tccagacccc agctgattgc catcatcacg ataagcgtaa tcagcggcag attctctttc
    19441 aggcgcgatg cttgtggctc gtcggcggcc tgctcatcgc gcagggaata gcccgctttt
    19501 tgcaccgcag attcaacctg ggcacggatg tcattgtcgg catcaaccac cagtttttcg
    19561 gtggcgaaca acacctgcac ctgattcacg cctgcaagct ggcgcacggc attttctacc
    19621 ttgcgcgcac aggcggcgca gtccatgccg ctgactttcc agctatagcg ggtgccggag
    19681 acgttttcag agagcgttgg cgagctggag catgcgccgt cgcagcaaca gtcgttggcg
    19741 ttctgtaccg tggttagcgg tttgaacgca gcaaattgag gggctttctt gccgtgattg
    19801 tcaggtgtcg acatggcatc ctccggttaa gttttttctc attaaccgaa ggatacactc
    19861 tggagtcgac tccagagtca agttttatca gagatacagt gagcggacga tgaggaagtg
    19921 accggcaaag tagcaggccg cagcaatcgc gttatccgca cggaagcgtc ggcgatagtg
    19981 gctccccagc cagacaaagt tactgataaa cagcaacgaa gcgccaacaa acgcagagag
    20041 cgccggagcg gtcggacgga agaaccacag ttcacctgcc agccacacca tcaccagcgt
    20101 catgccgata aacgtgcaga taggccaacg gtactcttcc agccgcgtcc agataatcgc
    20161 cagtagcagc gcacccagaa ccagcaacac cagtggcaga ggccagaaga aagagagcgt
    20221 catctgactg gcgaaataga tggtgtacag caggtgcgag aggaaaaacg cgccgatggc
    20281 gtacatcaga cgttgacgtg gcaacagggt tagcgcatcg cccagcagtg aggcgcacag
    20341 tcccgccagc accagatagc taatggcgtc gaacatcggt gcttgccagg ccagtaacag
    20401 caggagaaga agggttaacg gtttaaacac ccagcgttgc caggttggcc cacgatacga
    20461 tgcatccaca gatagccatg cggaaagaca gacagcgata aacgaccaaa gcatcttagt
    20521 tccttgaatt cgttattttt tcgtaagcag aatgcctacg gtcttgcttc cagtttagtg
    20581 gcgagggtgg acggatgaca acaccataac tggcagggta tgcttaattc cgcattttcc
    20641 gatgagggat aaagatgagt aaaccacctc ttttctttat tgttatcatt ggcttaattg
    20701 tcgtcgccgc atcgtttcgt tttatgcaac agcgacggga aaaagctgat aatgatatgg
    20761 ctccgctcca gcaaaagctg gtggtggtga gcaacaagcg ggaaaaaccg attaacgatc
    20821 gccgttcgcg ccagcaggaa gtgactccgg caggcaccag tatgcgctat gaggcaagct
    20881 tcaaaccgca aagcggagga atggagcaga cgtttcgcct cgacgcccag cagtaccacg
    20941 ccctgacagt gggcgataaa ggtacgctga gctataaagg aacgcgcttt gttagctttg
    21001 ttggcgaaca ataaggttat tttttaactt taaatttctt ctgccagacc agcagttcaa
    21061 acacgccgaa aaggaaaatc cgcacctttt cggcggtggt catctgcggg ccatctttcg
    21121 gtaaagtgga tttcaatagc gccagctgca taccatgcat cagcacggta aaaatcagcg
    21181 ccacgttaac gaaaatattt agcgggcgcg ggaagggatg caccagattg aggattaaaa
    21241 atccccaaac gcagagcatt aacaaacgac caatattaat cagcatcact ttctccttgt
    21301 gcttcgtgtt gatacagccg ataagccacc tgacccgcca ctttttcccg atgtaatgac
    21361 cagtttgccg gaacagtggg cagaccgttt tcgacttcgc tttcgacata aatcagggct
    21421 tcgtcagcca gccagccgtt atcttccagt aaatttatcg tctcttctaa caagccacgg
    21481 cggaacggtg gatcgacaaa cacgatatta tgcggtgtgc ctttttgcgc caggaatgac
    21541 attgcgttgc tgttcaccac gcgtgcattg cctgctttta gtgtcgccag attcttaatt
    21601 aactgctgag aaaccacgcg atccatctca atcaacgttg cccccgcagc gtagcgcgat
    21661 aacgcttcca gccccagcgc gccgctcccg gcgaagcaat ccagacattg ggcgtcaaca
    21721 atgaccggag ccagccagtt aaacaacgtt tcgcgtacgc ggtcggtggt ggggcgcaga
    21781 cctgggctat caggaaccgg gagtttacgg cctcgccact gcccgccaat aatgcggatt
    21841 tggccgctgc cggaatgatt cggttttttc atgataaatt gctcaatccg ccagataaca
    21901 catacttgca ggcggtgatg tgaattaagt taagtgatag actatttcat cattttttta
    21961 gctgctatgt acatagcgtt aacgctgtgc catgaagcaa cagcgaggag tgtagtcgca
    22021 aatggcgaaa gaaaaaagac gtggcttttt ttcctggctg ggctttggtc aaaaagagca
    22081 gaccccggaa aaagagacag aagttcagaa tgaacaaccg gttgtagaag aaatcgttca
    22141 ggcgcaagag cctgcgaagg cttctgaaca agccgttgaa gagcagccgc aggcgcatac
    22201 tgaagccgag gcggaaactt ttgctgccga cgttgtggaa gtcactgaac aggttgttga
    22261 aagtgaaaaa gcgcagcctg aagcggaagt tgttgcacag ccggaaccgg tcgtagaaga
    22321 aacgccggag ccagtggcta tcgaacgtga agagctgccg ttgccggaag acgtcaacgc
    22381 cgaagaggtt tcgtcagaag agtggcaggc tgaagcggaa accgtagaga ttgtcgaagc
    22441 ggcagaagaa gaagcggcta aagaagaaat caccgacgaa gagctggaag cacaggcgct
    22501 ggctgccgaa gcggcagaag aggcggtgat ggtagttcct ccggcagaag aagagcagcc
    22561 ggttgcagaa attgcccagg agcaagaaaa accgaccaaa gaaggcttct tcgcgcgcct
    22621 gaaacgcagc ctgttaaaaa ccaaagaaaa tctcggttcc ggatttatca gcctgttccg
    22681 cggtaaaaaa atcgacgatg atctgtttga agagctggaa gaacagctgt tgatcgccga
    22741 tgtgggcgtg gaaaccacac gtaaaattat caccaatctg acggaaggcg catcccgcaa
    22801 gcagcttcgt gatgccgagg cgctctatgg cctgctgaaa gaagagatgg gcgagattct
    22861 ggcgaaagtc gatgagccgc tgaatgttga agataaaacg ccgttcgtga tcctgatggt
    22921 gggcgtcaac ggtgtgggta aaaccacgac gattggtaag ctggcgcgtc agtttgagca
    22981 gcagggtaaa tcggtgatgc tggcggcggg cgatactttc cgtgcagctg cggttgaaca
    23041 gcttcaggtc tggggtcagc gcaacaatat tccggtgatt gcccagcata ccggtgcgga
    23101 ttccgcctct gttatcttcg acgccattca ggcagctaaa gcgcgtaata tcgacgtcct
    23161 gattgccgat accgccggac gtctgcagaa caaatcgcat ctgatggaag agttgaagaa
    23221 aatcgtccgc gtgatgaaga aactcgacgt tgaagcgccg catgaagtta tgctgactat
    23281 tgatgccagc accgggcaga acgcggtaag ccaggccaaa ctgttccacg aagctgttgg
    23341 cttaaccggc atcacgctaa cgaaactgga cggcacggcg aaaggcgggg taattttctc
    23401 ggtggctgac cagtttggta tccctatccg ctacattggt gtcggcgaac gtattgagga
    23461 tttgcgtccg tttaaggcgg acgactttat agaggcactt tttgcccgag aggattaaca
    23521 atgattcgct ttgaacatgt cagcaaggct tatctcggtg ggagacaggc gctgcagggc
    23581 gttacgttcc atatgcagcc gggtgagatg gcgtttctga ccggtcattc cggcgcaggg
    23641 aaaagtaccc tcctgaagct gatctgtggg attgagcggc ccagcgccgg gaaaatctgg
    23701 tttagcggcc atgacatcac gcgtctgaaa aaccgtgaag ttccgtttct gcgccgccag
    23761 attggcatga ttttccagga tcaccatcta ctgatggacc gtactgtcta cgataacgtg
    23821 gcgatcccgc tgattatcgc tggtgccagc ggtgacgata ttcgtcgccg ggtgtcggcg
    23881 gcactggata aagtcgggct actggacaaa gcgaagaact tccctattca gctttccggc
    23941 ggtgaacaac agcgtgttgg cattgcccgc gcggtggtga ataagcccgc ggtactgctg
    24001 gcggacgaac cgaccggtaa cctggacgac gcgttgtcgg aagggattct acgtctgttt
    24061 gaagagttta accgcgttgg ggtaaccgta ttgatggcaa cgcacgacat taatctgatt
    24121 tcacggcgtt cctatcgcat gctcaccctg agcgatggtc acttgcatgg aggcgtgggc
    24181 catgaataag cgcgatgcaa tcaatcatat tcggcagttt ggtgggcgtc tggatcgctt
    24241 ccgtaaatcg gtcggcggct caggcgacgg cggtcgtaac gcaccgaaac gcgcgaaatc
    24301 ctcgccaaaa ccggtaaatc gcaaaaccaa cgttttcaac gaacaggtgc gctatgcctt
    24361 ccacggcgca ttgcaggatc tgaaaagcaa accgttcgcc acgtttttaa cggtgatggt
    24421 tatcgccatc tccctgacgc tgcccagcgt ctgttatatg gtgtacaaaa acgttaatca
    24481 ggcggcgacg cagtattatc cgtcaccgca aatcactgtt tacttgcaaa aaacgctgga
    24541 cgatgacgcc gctgcgggcg tggtggcaca gttgcaggcc gagcaaggcg tggagaaagt
    24601 gaactatctt tctcgtgaag acgcactggg cgagttccgt aactggtctg gttttggtgg
    24661 tgcgctggat atgctggaag aaaacccgct tccggcagtg gcggtggtga tcccgaaact
    24721 cgattttcag gggacggaat cactcaatac gctgcgtgat cgtatcacgc agattaacgg
    24781 tattgacgaa gtgcggatgg atgacagctg gtttgcccgt ctggcggcgt tgaccgggct
    24841 ggttgggcgc gtttcggcga tgatcggcgt gttgatggtg gcggcagtgt tcctcgtcat
    24901 cggcaacagc gtacgtctga gcatctttgc tcgccgtgac tccatcaacg tgcagaaact
    24961 gattggtgcg acggatggtt tcatccttcg cccgttcctg tacggcggtg cgctgctggg
    25021 attttctggc gcattgttgt cattaatttt gtcagaaatt ctggtactgc gtttgtcgtc
    25081 agcggtggcg gaagtggcac aggttttcgg aacgaagttt gatatcaatg gtttatcgtt
    25141 cgatgaatgc ctgctattgc tgctggtatg ctcgatgatt ggctgggtgg cagcgtggct
    25201 tgccacggta caacatttac gccactttac gcctgaataa taaaagcgtg ttatactctt
    25261 tccctgcaat gggttccgta gcagggaaag agaccccgtt gtctcttccc ggtatttcat
    25321 ctctatgtca cattttgtgc gtaatttatt cacaagcttg cattgaactt gtggataaaa
    25381 tcacggtctg ataaaacagt gaatgataac ctcgttgctc ttaagctttg gcacagttgt
    25441 tgctaccact gaagcgccag aagatatcga ttgagaggat ttgaatgact gacaaaatgc
    25501 aaagtttagc tttagcccca gttggcaacc tggattccta catccgggca gctaacgcgt
    25561 ggccgatgtt gtcggctgac gaggagcggg cgctggctga aaagctgcat taccatggcg
    25621 atctggaagc agctaaaacg ctgatcctgt ctcacctgcg gtttgttgtt catattgctc
    25681 gtaattatgc gggctatggc ctgccacagg cggatttgat tcaggaaggt aacatcggcc
    25741 tgatgaaagc agtgcgccgt tttaacccgg aagtgggtgt gcgcctggtc tccttcgccg
    25801 tccactggat caaagcagag atccacgaat acgttctgcg taactggcgt atcgtcaaag
    25861 ttgcgaccac caaagcgcag cgcaaactgt tcttcaacct gcgtaaaacc aagcagcgtc
    25921 tgggctggtt taaccaggat gaagtcgaaa tggtggcccg tgaactgggc gtaaccagca
    25981 aagacgtacg tgagatggaa tcacgtatgg cggcacagga catgaccttt gacctgtctt
    26041 ccgacgacga ttccgacagc cagccgatgg caccggtgct ctatctgcag gataaatcat
    26101 ctaactttgc cgacggcatc gaagatgata actgggaaga gcaggcggca aaccgtctga
    26161 ccgacgcgat gcatggtctg gacgaacgca gccaggacat catccgtgcg cgctggctgg
    26221 acgaagacaa caagtccacg ttgcaggaac tggctgaccg ttacggtgtt tccgctgaac
    26281 gtgtgcgcca actggaaaag aacgcgatga aaaaacttcg cgccgctatt gaagcgtaat
    26341 ttccgctatt aagcagagaa ccctggatga gagtccgggg tttttgtttt ttgggcctct
    26401 acaataatca attccccctc cggcaaaacg ccaatcccca cgcagattgt taataaactg
    26461 tcaaaatagc tattccaata tcataaaaat cgggtatgtt ttagcagagt atgctgctaa
    26521 agcacgggta gtcatgcata aaacgaaata aagtgctgaa caacaacatc acaacacacg
    26581 taataaccag aagaatgggg attctcagga tgaacataaa gggtaaagcg ttactggcag
    26641 gatgtatcgc gctggcattc agcaatatgg ctctggcaga agatattaaa gtcgcggtcg
    26701 tgggtgcaat gtccggtccg gttgcgcagt acggtgacca ggagtttacc ggcgcagagc
    26761 aggcggttgc ggatatcaac gctaaaggcg gcattaaagg caacaaactg caaatcgtaa
    26821 aatatgacga tgcctgtgac ccgaaacagg cggttgcggt ggcgaacaaa gtcgttaacg
    26881 acggcattaa atatgtgatt ggtcacctct gttcttcatc aacgcagcct gcgtctgaca
    26941 tctacgaaga cgaaggcatt ttaatgatca ccccagcggc aaccgcgccg gagctgaccg
    27001 cccgtggcta tcagctgatc ctgcgcacca ccggcctgga ctccgaccag gggccgacgg
    27061 cggcgaaata tattcttgag aaagtgaaac cgcagcgtat tgctatcgtt cacgacaaac
    27121 agcaatacgg cgaaggtctg gcacgagcgg tgcaggacgg cctgaagaaa ggcaatgcta
    27181 acgtggtgtt ctttgatggt atcaccgccg gggaaaaaga tttctcaacg ctggtggcgc
    27241 gtctgaaaaa agagaatatc gacttcgttt actacggcgg ttatcacccg gaaatggggc
    27301 aaatcctgcg tcaggcacgc gcggcagggc tgaaaactca gtttatgggg ccggaaggtg
    27361 tggctaacgt ttcgctgtct aacattgcgg gcgaatcagc ggaagggctg ctggtgacca
    27421 agccgaagaa ctacgatcag gttccggcga acaaacccat tgttgatgcg atcaaagcga
    27481 aaaaacagga cccaagcggc gcattcgtct ggaccaccta cgccgcgctg caatctttgc
    27541 aggcgggcct gaatcagtct gacgatccgg ctgaaatcgc caaataccta aaagcgaact
    27601 ccgtggatac cgtgatgggg ccactgacct gggatgagaa aggcgatcta aagggctttg
    27661 agttcggcgt atttgactgg cacgccaacg gcacggcgac cgatgcgaag taattgttaa
    27721 tcggcaactt tgggttgccg ccaaattgct aatatcgaat acgttgcttc atgccggatg
    27781 cggcgtaaac gccttatccg gcctacaaga accaaagaaa tcagtaaatt gcagcacaca
    27841 ttataggcct gataagcgta gcgcatcagg caatttctcg ttggtcatca cttttgaaat
    27901 caacttgacg attaacgctt ctcccagccg ccctgctgtg ccgtaaatcc cagtgcctgc
    27961 ataaacgccg tcatcacacc gcgatcttcc acgcctgcat ccgccatcca ccagcatgaa
    28021 acgccaggat tgttacgcaa aacctcttcc agcagatatt gccccacacc gcgacggcgg
    28081 gtgacttccc gcacgcgtag agaatccagt gctccctcgg tgccgcttaa ggttacccgc
    28141 acggccgcaa gcagacgctc gttaaaacgc gcggcgtaga tacggtggtt atcgtcaacc
    28201 tgtaaagagg aaggggaata ctccggccag atcttttgca ggtcaatccg gtcctggtcg
    28261 ctaaagtttt ctaatcgaat gatggtcagc ttcatgggta acccgtgtaa atcacaaaag
    28321 tggaaccagt gtagcgaaat aatttaatcg gaggctttct cttttttatt tcttttggca
    28381 ggtgattaat tttttaacag caataattac aaagttaaaa cattagagaa tgaaaaatgt
    28441 ccagcataat cccctgaatg atagtgaatt attccgccct ttgtgccgtt attttatgct
    28501 gacaaaggca cttttttctg ttt
//
LOCUS       DAZSIO010000037        28183 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZSIO010000037 DAZSIO010000000
VERSION     DAZSIO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.037"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="ACHX5T_001461"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="ACHX5T_001461"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR8466718.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="ACHX5T_001462"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="ACHX5T_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR8466719.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="ACHX5T_001463"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="ACHX5T_001463"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR8466720.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="ACHX5T_001464"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="ACHX5T_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR8466721.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="ACHX5T_001465"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="ACHX5T_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR8466722.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="ACHX5T_001466"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="ACHX5T_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR8466723.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="ACHX5T_001467"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="ACHX5T_001467"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR8466724.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9168
                     /gene="atpI"
                     /locus_tag="ACHX5T_001468"
                     /pseudo
     CDS             8747..9168
                     /gene="atpI"
                     /locus_tag="ACHX5T_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
     gene            9177..9992
                     /gene="atpB"
                     /locus_tag="ACHX5T_001469"
     CDS             9177..9992
                     /gene="atpB"
                     /locus_tag="ACHX5T_001469"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR8466725.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            10039..10278
                     /gene="atpE"
                     /locus_tag="ACHX5T_001470"
     CDS             10039..10278
                     /gene="atpE"
                     /locus_tag="ACHX5T_001470"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR8466726.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10340..10810
                     /gene="atpF"
                     /locus_tag="ACHX5T_001471"
     CDS             10340..10810
                     /gene="atpF"
                     /locus_tag="ACHX5T_001471"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR8466727.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10825..11358
                     /gene="atpH"
                     /locus_tag="ACHX5T_001472"
     CDS             10825..11358
                     /gene="atpH"
                     /locus_tag="ACHX5T_001472"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR8466728.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11371..12912
                     /gene="atpA"
                     /locus_tag="ACHX5T_001473"
     CDS             11371..12912
                     /gene="atpA"
                     /locus_tag="ACHX5T_001473"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR8466729.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12963..13826
                     /gene="atpG"
                     /locus_tag="ACHX5T_001474"
     CDS             12963..13826
                     /gene="atpG"
                     /locus_tag="ACHX5T_001474"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR8466730.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13853..15235
                     /gene="atpD"
                     /locus_tag="ACHX5T_001475"
     CDS             13853..15235
                     /gene="atpD"
                     /locus_tag="ACHX5T_001475"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR8466731.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15256..15675
                     /locus_tag="ACHX5T_001476"
     CDS             15256..15675
                     /locus_tag="ACHX5T_001476"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR8466732.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            16026..17396
                     /gene="glmU"
                     /locus_tag="ACHX5T_001477"
     CDS             16026..17396
                     /gene="glmU"
                     /locus_tag="ACHX5T_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR8466733.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17558..19387
                     /gene="glmS"
                     /locus_tag="ACHX5T_001478"
     CDS             17558..19387
                     /gene="glmS"
                     /locus_tag="ACHX5T_001478"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR8466734.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19549..20370
                     /locus_tag="ACHX5T_001479"
     CDS             19549..20370
                     /locus_tag="ACHX5T_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFR8466735.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20357..22465
                     /locus_tag="ACHX5T_001480"
     CDS             20357..22465
                     /locus_tag="ACHX5T_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR8466736.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22462..24129
                     /locus_tag="ACHX5T_001481"
     CDS             22462..24129
                     /locus_tag="ACHX5T_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR8466737.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            24132..25658
                     /locus_tag="ACHX5T_001482"
     CDS             24132..25658
                     /locus_tag="ACHX5T_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFR8466738.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            25659..27275
                     /locus_tag="ACHX5T_001483"
     CDS             25659..27275
                     /locus_tag="ACHX5T_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFR8466739.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(27331..27483)
                     /locus_tag="ACHX5T_001484"
                     /pseudo
     CDS             complement(27331..27483)
                     /locus_tag="ACHX5T_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(27506..27883)
                     /locus_tag="ACHX5T_001485"
     CDS             complement(27506..27883)
                     /locus_tag="ACHX5T_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466740.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
BASE COUNT         7297 a         6549 c         7273 g         7064 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gagtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcagtctt tctgcctaac
     8941 gttttgttta tgatatttgc ctggcgtcac caggcgcata caccagcgaa aggccgggtg
     9001 gcttggacat tcgcatttgg cgaagctttc aaagttctgg cgatgttggt gttactggtg
     9061 gtggcgttgg cggttttaaa ggcggtattc ttgccgctga tcgttacgtg ggttttggtg
     9121 ctggtggttc agatactggc accggctgta attaacaaca aagggtaaaa ggcatcatgg
     9181 cttcagaaaa tatgacgccg caggattaca taggacacca cctgaataac cttcagctgg
     9241 acctgcgtac attctcgctg gtggatccac aaaacccccc agccaccttc tggacaatca
     9301 atattgactc catgttcttc tcggtggtgc tgggtctgtt gttcctggtt ttattccgta
     9361 gcgtagccaa aaaggcgacc agcggtgtgc caggtaagtt tcagaccgcg attgagctgg
     9421 tgatcggctt tgttaatggt agcgtgaaag acatgtacca tggcaaaagc aagctgattg
     9481 ctccgctggc cctgacgatc ttcgtctggg tattcctgat gaatctgatg gatttactgc
     9541 ctatcgactt gctgccgtac attgctgaac atgtactggg tctgcctgca ctgcgtgtgg
     9601 ttccgtctgc ggacgtgaac gtaacgctgt ctatggcact gggcgtattt atcctgattc
     9661 tgttctacaa catcaaaatg aaaggcatcg gcggcttcac gaaagagttg acgctgcagc
     9721 cgttcaatca ctgggcgttc attcctgtca acttaatcct tgaaggggta agcctgctgt
     9781 ccaaaccagt ttcactcggt ttgcgactgt tcggtaacat gtatgccggt gagctgattt
     9841 tcattctgat tgctggtctg ttgccgtggt ggtcacagtg gatcctgaat gtgccgtggg
     9901 ccattttcca catcctgatc attacgctgc aagccttcat cttcatggtt ctgacgatcg
     9961 tctatctgtc gatggcgtct gaagaacatt aatttaccaa cactactacg ttttaactga
    10021 aacaaactgg agactgtcat ggaaaacctg aatatggatc tgctgtacat ggctgccgct
    10081 gtgatgatgg gtctggcggc aatcggtgct gcgatcggta tcggcatcct cgggggtaaa
    10141 ttcctggaag gcgcagcgcg tcaacctgat ctgattcctc tgctgcgtac tcagttcttt
    10201 atcgttatgg gtctggtgga tgctatcccg atgatcgctg taggtctggg tctgtacgtg
    10261 atgttcgctg tcgcgtagta agcgttgctt ttatttaaag agcaatatca gaacgttaac
    10321 taaatagagg cattgtgctg tgaatcttaa cgcaacaatc ctcggccagg ccatcgcgtt
    10381 tgtcctgttc gttctgttct gcatgaagta cgtatggccg ccattaatgg cagccatcga
    10441 aaaacgtcaa aaagaaattg ctgacggcct tgcttccgca gaacgagcac ataaggacct
    10501 tgaccttgca aaggccagcg cgaccgacca gctgaaaaaa gcgaaagcgg aagcccaggt
    10561 aatcatcgag caggcgaaca aacgccgctc gcagattctg gacgaagcga aagctgaggc
    10621 agaacaggaa cgtactaaaa tcgtggccca ggcgcaggca gaaattgaag ccgagcgtaa
    10681 acgtgcccgt gaagagctgc gtaagcaagt tgctatcctg gctgttgctg gcgccgagaa
    10741 gatcatcgaa cgttccgtgg atgaagctgc taacagcgac atcgtggata aacttgtcgc
    10801 tgaactgtaa ggagggaggg gctgatgtct gaatttatta cggtagctcg cccctacgcc
    10861 aaagcagctt ttgactttgc cgtcgaacac caaagtgtag aacgctggca ggacatgctg
    10921 acgtttgccg ccgaggtaac caaaaacgaa caaatggcag agcttctctc tggcgcgctt
    10981 gcgccagaaa cgctcgccga gtcgtttatc gcagtttgtg gtgagcaact ggacgaaaac
    11041 ggtcagaacc tgattcgggt aatggctgaa aatggtcgtc ttaacgcgct cccggatgtt
    11101 ctggagcagt ttattcacct tcgtgccgtg agtgaggcta ccgctgaggt agacgtcatt
    11161 tccgctgccg cactgagtga acaacagctc gcgaaaattt ctgctgcgat ggaaaaacgt
    11221 ctgtcacgca aagttaagct gaattgcaaa atcgataagt ctgtaatggc tggcgttatc
    11281 atccgagcgg gtgatatggt cattgatggc agcgtacgcg gtcgtcttga gcgccttgca
    11341 gacgtcttgc agtcttaagg ggactggagc atgcaactga attccaccga aatcagcgaa
    11401 ctgatcaagc agcgcattgc tcagttcaat gttgtgagtg aagctcacaa cgaaggtact
    11461 attgtttctg taagtgacgg tgttatccgc attcacggcc tggccgattg tatgcagggt
    11521 gaaatgatct ccctgccggg taaccgttac gctatcgcac tgaacctcga gcgcgactct
    11581 gtaggtgcgg ttgttatggg tccgtacgct gaccttgccg aaggcatgaa agttaagtgt
    11641 actggccgta tcctggaagt tccggttggc cgtggcctgc tgggccgtgt ggttaacact
    11701 ctgggtgcac caatcgacgg taaaggtccg ctggatcacg acggcttctc tgctgtagaa
    11761 gcaatcgctc cgggcgttat cgaacgtcag tccgtagatc agccggtaca gaccggttat
    11821 aaagccgttg actccatgat cccaatcggt cgtggtcagc gtgaattgat catcggtgac
    11881 cgtcagacag gtaaaaccgc actggctatc gatgccatca tcaaccagcg cgattccggt
    11941 atcaaatgta tctatgtcgc tatcggccag aaagcgtcca ccatttctaa cgtggtacgt
    12001 aaactggaag agcacggcgc actggctaac accatcgttg tggtagcaac cgcgtctgaa
    12061 tccgctgcac tgcaatacct ggcaccgtat gccggttgcg caatgggcga atacttccgt
    12121 gaccgcggtg aagatgcgct gatcatttac gatgacctgt ctaaacaggc tgttgcttac
    12181 cgtcagatct ccctgctgct ccgtcgtccg ccaggacgtg aagcattccc gggcgacgtt
    12241 ttctacctcc actctcgtct gctggagcgt gctgcacgtg ttaacgccga atacgttgaa
    12301 gccttcacca aaggtgaagt gaaagggaaa accggttctc tgaccgcact gccgattatc
    12361 gaaactcagg cgggtgacgt ttctgcgttc gttccgacca acgtaatctc cattaccgat
    12421 ggtcagatct tcctggaaac caacctgttc aacgccggta ttcgtcctgc ggttaacccg
    12481 ggtatttccg tatcccgtgt tggtggtgca gcacagacca agatcatgaa aaaactgtcc
    12541 ggtggtatcc gtaccgctct ggcacagtat cgtgaactgg cagcgttctc tcagtttgca
    12601 tccgaccttg acgatgcaac acgtaagcag cttgaccacg gtcagaaagt gaccgaactg
    12661 ctgaaacaga aacagtatgc gccgatgtcc gttgcgcagc agtctctggt tctgttcgca
    12721 gcagaacgtg gttacctggc ggatgttgaa ctgtcgaaaa ttggcagctt cgaagccgct
    12781 ctgctggctt acgtcgaccg tgatcacgct ccgttgatgc aagagatcaa ccagaccggt
    12841 ggctacaacg acgaaatcga aggcaagctg aaaggcatcc tcgattcctt caaagcaacc
    12901 caatcctggt aacgtctgga ggcttgcctt agggcaggcc gcaaggcatt gaggagaagc
    12961 tcatggccgg cgcaaaagag atacgtagta agatcgcaag cgtccagaac acgcaaaaga
    13021 tcactaaagc gatggagatg gtcgccgctt ccaaaatgcg taaatcgcag gatcgcatgg
    13081 cggccagccg tccttatgca gaaaccatgc gcaaagtgat tggtcacctt gcacacggta
    13141 atctggaata taagcaccct tacctggaag accgcgacgt taaacgcgtg ggctacctgg
    13201 tggtgtcgac cgaccgtggt ttgtgcggtg gtttgaacat taacctgttc aaaaaactgc
    13261 tggcggaaat gaagacctgg accgacaaag gcgttcaatg cgacctcgca atgatcggct
    13321 cgaaaggcgt gtcgttcttc aactccgtgg gcggcaatgt tgttgcccag gtcaccggca
    13381 tgggggataa cccttccctg tccgaactga tcggtccggt aaaagtgatg ttgcaggcct
    13441 acgacgaagg ccgtctggac aagctttaca ttgtcagcaa caaatttatt aacaccatgt
    13501 ctcaggttcc gaccatcagc cagctgctgc cgttaccggc atcagatgat gatgatctga
    13561 aacataaatc ctgggattac ctgtacgaac ccgatccgaa ggcgttgctg gataccctgc
    13621 tgcgtcgtta tgtcgaatct caggtttatc agggcgtggt tgaaaacctg gccagcgagc
    13681 aggccgcccg tatggtggcg atgaaagccg cgaccgacaa tggcggcagc ctgattaaag
    13741 agctgcagtt ggtatacaac aaagctcgtc aggccagcat tactcaggaa ctcaccgaga
    13801 tcgtctcggg ggccgccgcg gtttaaacag gttatttcgt agaggattta agatggctac
    13861 tggaaagatt gtccaggtaa tcggcgccgt agttgacgtc gaattccctc aggatgccgt
    13921 accgcgcgtg tacgatgctc ttgaggtgca aaatggtaat gagcgtctgg tgctggaagt
    13981 tcagcagcag ctcggcggcg gtatcgtgcg taccatcgca atgggttcct ccgacggtct
    14041 gcgtcgcggt ctggatgtaa aagacctcga acacccgatc gaagtcccgg taggtaaagc
    14101 gactctgggc cgtatcatga acgtactggg tgaaccggtc gacatgaaag gcgagatcgg
    14161 tgaagaagag cgttgggcga ttcaccgcgc agcaccttcc tacgaagagc tgtcaaactc
    14221 tcaggaactg ctggaaaccg gtatcaaagt tatcgacctg atgtgtccgt tcgctaaggg
    14281 cggtaaagtt ggtctgttcg gtggtgcggg tgtaggtaaa accgtaaaca tgatggagct
    14341 cattcgtaac atcgcgatcg agcactccgg ttactctgtg tttgcgggcg taggtgaacg
    14401 tactcgtgag ggtaacgact tctaccacga aatgaccgac tccaacgtta tcgacaaagt
    14461 atccctggtg tatggccaga tgaacgagcc gccgggaaac cgtctgcgcg ttgctctgac
    14521 cggtctgacc atggctgaga aattccgtga cgaaggtcgt gacgttctgc tgttcgttga
    14581 caacatctat cgttacaccc tggccggtac ggaagtatcc gcactgctgg gccgtatgcc
    14641 ttcagcggta ggttatcagc cgaccctggc ggaagagatg ggcgttctgc aggaacgtat
    14701 cacctccacc aaaactggtt ctatcacctc cgtacaggcg gtatacgtac ctgcggatga
    14761 cttgactgac ccgtctccgg caaccacctt tgcgcacctt gacgcaaccg tggtactgag
    14821 ccgtcagatc gcgtctctgg gtatctaccc ggccgttgac ccgctggact ccaccagccg
    14881 tcagctggac ccgctggtgg ttggtcagga acactacgac accgcgcgtg gcgttcagtc
    14941 catcctgcaa cgttatcagg aactgaaaga catcatcgcc atcctgggta tggatgaact
    15001 gtctgaagaa gacaaactgg tggtagcgcg tgctcgtaag atccagcgct tcctgtccca
    15061 gccgttcttc gtggcagaag tattcaccgg ttctccgggt aaatacgtct ccctgaaaga
    15121 caccatccgt ggctttaaag gcatcatgga aggcgaatac gatcacctgc cggagcaggc
    15181 gttctacatg gtcggttcca tcgaagaagc tgtggaaaaa gccaaaaaac tttaacgcct
    15241 taatcggagg gtgatatggc aatgacttac cacctggacg tcgtcagcgc agagcaacaa
    15301 atgttctctg gtctggtcga gaaaatccag gtaacgggta gcgaaggtga actggggatc
    15361 taccctggcc acgcaccgct gctcaccgcc attaagcctg gtatgattcg catcgtgaaa
    15421 cagcacggtc acgaagagtt tatctatctg tctggcggca ttcttgaagt gcagcctggc
    15481 aacgtgaccg ttctggccga caccgcaatt cgcggccagg atctcgacga agcgcgagcc
    15541 atggaagcga aacgtaaggc tgaagagcac attagcagct ctcacggcga cgtagattac
    15601 gctcaggcgt ctgcggaact ggccaaagcg atcgcgcagc tgcgcgttat cgagttgacc
    15661 aaaaaagcga tgtaacaccg gcttgaaaag cacaaaagcc agtctggaaa caggctggct
    15721 ttttttacgc gttgacccgt cctgaagagc gttcacatag atcctgctga tataaaacac
    15781 ccctgttttc ccgtttattc attgatcgaa ataagagcaa aaacatccac ctgacgctta
    15841 aatgaaggta ctgccttaat tttctgcaga taaaaggcgt gacgatggtc gaaaatggca
    15901 ctttcgtcag cggggataat ccgttattga acaatttatc ctctgtccat ttcacgatga
    15961 aaaaaatgta gttttttcaa ggtgaagcgg tttaaattcg ttctcaaatt acagtcagga
    16021 cgcgtatgtt gaataatgct atgagcgtag tgatccttgc cgcaggcaaa ggcacgcgca
    16081 tgtattccga tcttccgaaa gtgctgcata cccttgccgg gaaagcgatg gttcagcatg
    16141 tcattgatgc tgcgaatgaa ttaggcgcag cgcacgttca cctggtgtac ggtcacggcg
    16201 gcgatctgct taaacaggcg ctgaaagacg acaaccttaa ctgggtgctt caggcagagc
    16261 agctgggtac gggtcatgca atgcagcagg ccgcaccttt ctttgccgat gatgaagaca
    16321 ttttaatgct ctacggcgac gtgccgctga tctctgtcga aacactccag cgcctgcgtg
    16381 atgctaaacc ccagggtggc attggtctgc tgacggtgaa actggatgat ccgaccggtt
    16441 atggacgtat cacccgtgaa aatggcaaag ttaccggcat tgttgagcac aaagatgcca
    16501 ccgacgagca gcgtcagatt caggagatca acaccggcat tctgattgcc aacggcgcag
    16561 atatgaaacg ctggctggcg aagctgacca acaataatgc tcagggcgaa tactacatca
    16621 ccgacattat tgcgctggcg tatcaggaag ggcgtgaaat cgtcgccgtt catccgcaac
    16681 gtttaagcga agtagaaggt gtgaataacc gcctgcaact ctcccgtctg gagcgcgttt
    16741 accagtccga acaggctgaa aaactgctgt tagcaggcgt tatgctgcgc gatccggcgc
    16801 gttttgatct gcgcggtacg cttactcacg ggcgcgatgt tgaaattgat actaacgtta
    16861 tcatcgaggg caacgtgact ctcggtcatc gcgtgaaaat cggcaccggt tgcgtgatta
    16921 aaaacagcgt gattggcgat gattgcgaaa tcagtccgta taccgttgtg gaagacgcga
    16981 atctggcagc ggcctgtact attggcccgt ttgcccgtct gcgtcctggt gctgagttgc
    17041 tggaaggtgc acacgttggt aacttcgttg agatgaaaaa agcgcgtctg ggtaaaggca
    17101 cgaaagccgg gcatctgact tacctgggcg atgcggaaat tggcgataac gttaacatcg
    17161 gcgcgggaac cattacctgc aactacgatg gtgcgaataa atttaagacc attatcggcg
    17221 acgatgtgtt tgtcggttcc gacactcagc tggtggcccc ggtaacagta ggcaaaggcg
    17281 cgaccattgc tgcgggtaca actgtgacgc gtaatgtcgg cgaaaatgca ttagctatca
    17341 gccgtgtgcc gcagactcag aaagaaggct ggcgtcgtcc ggtaaagaaa aagtaattct
    17401 ggccgggtaa tccggtcatc tgggatgagg agataacata atctccctcc cacaagcagt
    17461 aactataaaa ataaccccac tctctacaag gctcggggcg cccgaaaaaa cgggcataca
    17521 ggttgaccga caacgatata aatcggaatc aaaaactatg tgtggaattg ttggcgcgat
    17581 cgcgcaacgt gatgtagcag aaatccttct tgaaggttta cgtcgtctgg aataccgcgg
    17641 atatgactct gccggtctgg ccgttgttga tgcggaaggt catatgactc gcctgcgtcg
    17701 cctcggtaaa gtccagatgc tggctcaggc agcggaagaa catcctctgc atggcggcac
    17761 cggtattgct cacactcgct gggcgaccca cggtgaacct tcagaagcta acgcgcatcc
    17821 gcatgtttct gaacacattg tggtggtgca taatggcatc atcgaaaacc atgaaccgct
    17881 gcgtgaagag ttaaaagcgc gtggctatac cttcgtttct gaaaccgaca ccgaagtgat
    17941 tgcccatctg gtgaactggg agctgaaaca aggcgggact ctgcgtgagg ctgttctgcg
    18001 tgctatcccg cagctgcgcg gtgcgtacgg tacagtgatc atggactccc gtcacccgga
    18061 taccctgcta gcggcacgtt ctggtagtcc gctggtgatt ggcctgggta tgggcgaaaa
    18121 ctttatcgct tctgaccagc tggcgctgtt gccggtgacc cgtcgcttta tcttccttga
    18181 agagggcgat attgcggaaa tcactcgccg ttcggtaaac atcttcgata aaactggcgc
    18241 ggaagtaaaa cgtcaggata tcgaatccaa tctgcaatat gacgcgggcg ataaaggtat
    18301 ttaccgtcac tacatgcaga aagagatcta cgaacagccg aacgcgataa aaaacaccct
    18361 taccgggcgc atcagccacg gtcaggttga tttaagcgag ctgggaccga acgccgacga
    18421 actgctgtcg aaggttgagc atattcagat cctcgcctgt ggtacttctt ataactccgg
    18481 tatggtttcc cgctactggt ttgaatcgct ggcaggtatt ccgtgcgacg tcgaaatcgc
    18541 ctctgaattc cgctatcgta aatctgccgt gcgtcgtaac agcctgatga tcaccttgtc
    18601 acagtctggc gaaaccgcgg ataccctggc gggcctgcgt ctgtcgaaag agctgggtta
    18661 ccttggttcg ctggcaatct gtaacgttcc gggttcttct ctggtgcgcg aatccgatct
    18721 ggcgctgatg accaacgcgg gtacggaaat cggcgtggcg tccactaaag cattcaccac
    18781 tcagttaact gtgctgttga tgctggtggc gaagctgtct cgcctgaaag gtctggatgc
    18841 ctccattgaa catgacattg tgcatggtct gcaggcgctg ccgagccgta ttgagcagat
    18901 gctgtctcag gataaacgca ttgaagctct ggcagaagat ttctctgaca aacatcacgc
    18961 gctgttcctg ggccgtggcg atcagtaccc aatcgcgctg gaaggcgcat tgaagctgaa
    19021 agagatctct tacattcacg ctgaagccta cgctgcaggc gagctgaaac acggcccgct
    19081 ggcgctgatt gacgcggata tgccggttat cgtcgttgca ccgaacaacg aactgttgga
    19141 aaaactgaaa tccaacattg aagaagttcg cgcgcgtggc ggtcaattgt atgtcttcgc
    19201 cgatcaggat gcgggttttg taagtagcga taacatgcac atcatcgaga tgccgcatgt
    19261 ggaagaggtg attgcaccga tcttctacac cgttccgctg cagctgctgg cttaccatgt
    19321 cgcgctgatc aaaggcaccg acgttgacca gccgcgtaac ctggcaaaat cggttacggt
    19381 tgagtaatca ctatcataga taaaaaaggg ctgctgtggg cggacaataa agtcttaaac
    19441 tgaacaaaat agatctaaac tatgacaata aagtcttaaa ctagacagaa tagttgtaaa
    19501 ctgaaatcag tccagttatg ctgtgaaaaa gcatactgga cttttgttat ggctaaagca
    19561 aactcttcat tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg tggccaaggg
    19621 catggtaaag actatattcc atggctaaca gtacaagaag ttccttcttc aggtcgttcc
    19681 caccgtattt attctcataa gacgggacga gtccatcatt tgctatctga cttagagctt
    19741 gctgtttttc tcagtcttga gtgggagagc agcgtgctag atatacgcga gcagttcccc
    19801 ttattaccta gtgataccag gcagattgca atagatagtg gtattaagca tcctgttatt
    19861 cgtggtgtag atcaggttat gtctactgat tttttagtgg actgcaaaga tggtcctttt
    19921 gagcagtttg ctattcaagt caaacctgca gcagccttac aagacgagcg taccttagaa
    19981 aaactagaac tagagcgtcg ctattggcag caaaagcaaa ttccttggtt catttttact
    20041 gataaagaaa taaatcccgt agtaaaagaa aatattgaat ggctttattc agtgaaaaca
    20101 gaagaagttt ctgcggagct tttagcacaa ctatccccat tggcccatat cctgcaagaa
    20161 aaaggagatg aaaacattat caatgtctgt aagcaggttg atattgctta tgatttggag
    20221 ttaggcaaaa cattgagtga gatacgagcc ttaaccgcaa atggttttat taagttcaat
    20281 atttataagt ctttcagggc aaataagtgt gcagatctct gtattagcca agtagtgaat
    20341 atggaggagt tgcgctatgt ggcaaattaa tgaggttgtg ctatttgata atgatccgta
    20401 tcgcattttg gctatagagg atggccaagt tgtctggatg caaataagcg ctgataaagg
    20461 agttccacaa gctagggctg agttgttgct aatgcagtat ttagatgaag gccgcttagt
    20521 tagaactgat gacccttatg tacatcttga tttagaagag ccgtctgtag attctgtcag
    20581 cttccagaag cgcgaggagg attatcgaaa aattcttcct attattaata gtaaggatcg
    20641 tttcgaccct aaagtcagaa gcgaactcgt tgagcatgtg gtccaagaac ataaggttac
    20701 taaggctaca gtttataagt tgttacgccg ttactggcag cgtggtcaaa cgcctaatgc
    20761 attaattcct gactacaaaa acagcggtgc accaggggaa agacgttcag cgacaggaac
    20821 agcaaagatt ggccgagcca gagaatatgg taagggtgaa ggaaccaagg taacgcccga
    20881 gattgaacgc ctttttaggt tgaccataga aaagcacctg ttaaatcaaa aaggtacaaa
    20941 gaccaccgtt gcctatagac gatttgtgga cttgtttgct cagtattttc ctcgcattcc
    21001 ccaagaggat tacccaacac tacgtcagtt tcgttatttt tatgatcgag aataccctaa
    21061 agctcagcgc ttaaagtcta gagttaaagc aggggtatat aaaaaagacg tacgaccctt
    21121 aagtagtaca gccacttctc aggcgttagg ccctgggagt cgttatgaga ttgatgccac
    21181 gattgctgat atttatttag tggatcatca tgatcgccaa aaaatcatag gaagaccaac
    21241 gctttacatt gtgattgatg tgtttagtcg gatgatcacg ggcttttata tcggctttga
    21301 aaatccgtct tatgtggtgg cgatgcaggc ttttgtaaat gcttgctctg acaaaacggc
    21361 catttgtgcc cagcatgata ttgagattag tagctcagac tggccgtgtg taggtttgcc
    21421 agatgtgttg ctagcggacc gtggcgaatt aatgagtcat caggtcgaag ccttagtttc
    21481 tagttttaat gtgcgagtgg aaagtgctcc acctagacgt ggcgatgcta aaggcatagt
    21541 ggaaagcact tttagaacac tacaagccga gtttaagtcc tttgcacctg gcattgtaga
    21601 gggcagtcgg atcaaaagcc atggtgaaac agactatagg ttagatgcat ctctgtcggt
    21661 atttgagttc acacaaatta ttttgcgtac gatcttattc agaaataacc atctggtgat
    21721 ggataaatac gatcgagatg ctgattttcc tacagattta ccgtctattc ctgtccagct
    21781 atggcaatgg ggtatgcagc atcgtacagg tagtttaagg gctgtggagc aagagcagtt
    21841 gcgagtagcg ttactgcctc gccgaaaggt ctctatttct tcatttggcg ttaatttgtg
    21901 gggtttgtat tactcggggt cagagattct gcgtgagggt tggttgcagc ggagcactga
    21961 tatagctaga cctcaacatt tagaagcggc ttatgaccca gtgctggttg atacgattta
    22021 tttgtttccg caagttggca gccgtgtatt ttggcgctgt aatctgacgg aacgtagtcg
    22081 gcagtttaaa ggtctctcat tttgggaggt ttgggatata caagcacaag aaaaacacaa
    22141 taaagccaat gcgaagcagg atgagttaac taaacgcagg gagcttgagg cgtttattca
    22201 gcaaaccatt cagaaagcga ataagttaac gcccagtact actgagccca aatcaacacg
    22261 cattaagcag attaaaacta ataaaaaaga agccgtgacc tcggagcgta aaaaacgtgc
    22321 ggagcatttg aagccaagct cttcaggtga tgaggctaaa gttattcctt tcaacgcagt
    22381 ggaagcggat gatcaagaag attacagcct acccacatac gtgcctgaat tatttcagga
    22441 tccaccagaa aaggatgagt catgagtgct acccggattc aagcagttta tcgtgatacg
    22501 ggggtagagg cttatcgtga taatcctttt atcgaggcct taccaccatt acaagagtca
    22561 gtgaatagtg ctgcatcact gaaatcctct ttacagctta cttcctctga cttgcaaaag
    22621 tcccgtgtta tcagagctca taccatttgt cgtattccag atgactattt tcagccatta
    22681 ggtacgcatt tgctactaag tgagcgtatt tcggtcatga ttcgaggtgg ctacgtaggc
    22741 agaaatccta aaacaggaga tttacaaaag catttacaaa atggttatga gcgtgttcaa
    22801 acgggagagt tggagacatt tcgctttgag gaggcacgat ctacggcaca aagcttattg
    22861 ttaattggtt gttctggtag tgggaagacg acctctcttc atcgtattct agccacgtat
    22921 cctcaggtga tttaccatcg tgaactcaat gtagagcagg tggtgtattt gaaaatagac
    22981 tgctcgcata atggttcgct aaaagaaatc tgcttgaatt ttttcagagc gttggatcga
    23041 gccttgggct cgaactatga gcgtcgttat ggcttaaaac gtcatggtat agaaaccatg
    23101 ttggctttga tgtcgcaaat agccaatgca catgctttag ggttgttggt tattgatgaa
    23161 attcagcatt taagccgctc tcgttcgggt ggatctcaag agatgctgaa cttttttgtg
    23221 acgatggtga atattattgg cgtaccagtg atgttgattg gtacccctaa agcacgagag
    23281 atttttgagg ctgatttgcg gtctgcacgt agaggggcag ggtttggagc tatattctgg
    23341 gatcctatac aacaaacgca acgtggaaag cccaatcaag agtggatcgc ttttacggat
    23401 aatctttggc aattacagct tttacaacgc aaagatgcgc tgttatcgga tgaggtccgt
    23461 gatgtgtggt atgagctaag ccaaggagtg atggacattg tagtaaaact ttttgtactc
    23521 gctcagctcc gtgcgctagc tttaggcaat gagcgtatta ccgctggttt attgcggcaa
    23581 gtgtatcaag atgagttaaa gcctgtgcac cccatgctag aggcattacg ctcgggtatc
    23641 ccagaacgca ttgctcgtta ttctgatcta gtcgttcccg agattgataa acggttaatc
    23701 caacttcagc tagatatcgc agcgatacaa gaacaaacac cagaagaaaa agcccttcaa
    23761 gagttagata ccgaagatca gcgtcattta tatctgatgc tgaaagagga ttacgattca
    23821 agcctgttaa ttcccactat taaaaaagcg tttagccaga atccaacgat gacaagacaa
    23881 aagttactgc ctcttgtttt gcagtggttg atggaaggcg aaacggtagt gtcagaacta
    23941 gaaaagccct ccaagagtaa aaaggtttcg gctataaagg tagtcaagcc cagcgactgg
    24001 gatagcttgc ctgatacgga tttacgttat atctattcac aacgccaacc tgaaaaaacc
    24061 atgcatgaac ggttaaaagg gaaaggggta atagtggata tggcgagctt atttaaacaa
    24121 gcaggttagc catgagaaac tttcctgttc cgtactcgaa tgagctgatt tatagcacta
    24181 ttgcacgggc aggcgtttat caagggattg ttagtcctaa gcagctgttg gatgaggtgt
    24241 atggcaaccg caaggtggtc gctaccttag gtctgccctc gcatttaggt gtgatagcaa
    24301 gacatctaca tcaaacagga cgttacgctg ttcagcagct tatttatgag cataccttat
    24361 tccctttata tgctccgttt gtaggcaagg agcgccgaga cgaagctatt cggttaatgg
    24421 agtaccaagc gcaaggtgcg gtgcatttaa tgctaggagt cgctgcttct agagttaaga
    24481 gcgataaccg ctttagatac tgccctgatt gcgttgctct tcagctaaat aggtatgggg
    24541 aagccttttg gcaacgagat tggtatttgc ccgctttgcc atattgtcca aaacacggtg
    24601 ctttagtctt ctttgataga gctgtagatg atcaccgaca tcaattttgg gctttgggtc
    24661 atactgagct gctttcagac taccccaaag actccctatc tcaattaaca gcactagctg
    24721 cttatatagc ccctctgtta gatgctccac gagcgcaaga gctttcccca agccttgagc
    24781 agtggacgct gttttatcag cgcttagcgc aggatctagg gctaaccaaa agcaagcaca
    24841 ttcgtcatga cttggtggcg gagagagtga ggcagacttt tagtgatgag gcactagaga
    24901 aactggattt aaagttggca gagaacaagg acacgtgttg gctgaaaagt atattccgta
    24961 agcatagaaa agcctttagt tatttacagc atagtattgt gtggcaagcc ttattgccaa
    25021 aactaacggt tatagaagcg ctacagcagg caagtgctct tactgagcac tctataacga
    25081 caagacctgt tagccagtct gtgcaaccta actctgaaga tttatctgtt aagcataaag
    25141 actggcagca actagtgcat aaataccaag gaattaaggc ggcaagacag tctttagagg
    25201 gtggggtgct atacgcttgg ctttaccgac atgacaggga ttggctagtt cactggaatc
    25261 aacagcatca acaagagcgt ctggcacccg cccctagagt tgattggaac caaagagatc
    25321 gaattgctgt acgacaacta ttaagaatca taaagcgtct agatagtagc cttgatcacc
    25381 caagagcgac atcgagctgg ctgttaaagc aaactcctaa cggaacctct cttgcaaaaa
    25441 atctacagaa actgcctttg gtagcgcttt gcttaaagcg ttactcagag agtgtggaag
    25501 attatcaaat tagacggatt agccaagctt ttattaagct taaacaggaa gatgttgagc
    25561 ttaggcgctg gcgattatta agaagtgcaa cgttatctaa agagcggata actgaggaag
    25621 cacaaagatt cttggaaatg gtttatgggg aagagtgagt ggttaggcta gctacattta
    25681 atgacaatgt gcaggttgta catattggtc atttattccg taactcgggt cataaggagt
    25741 ggcgtatttt tgtttggttt aatccaatgc aagaacggaa atggactcga tttactcatt
    25801 tgcctttatt aagtcgagct aaggtggtta acagtacaac aaagcaaata aataaggcgg
    25861 atcgtgtgat tgagtttgaa gcatcggatc ttcaacgagc caaaataatc gattttccta
    25921 atctctcgtc ctttgcttcc gtacgcaaca aggatggagc gcagagttca tttatttacg
    25981 aagctgaaac accatatagc aagactcgtt atcacatccc acagttagag ctagctcggt
    26041 cattattttt aattaactcc tatttctgtc gaagctgttt gagcagtacc gctttacagc
    26101 aagagttcga cgttcagtat gaggttgagc gagatcattt agagataagg atcttaccca
    26161 gttcatcgtt tcctaaaggg gcgttagagc tgtcggccgt agtgcagctt ttggtttggt
    26221 tgttttcgga tcaagatgtt atggattcgt atgaaagtat ttttaggcac tatcaacaaa
    26281 atagagaaat taagaacggc gttgaaagct ggtgctttag ctttgaccct ccgcccatgc
    26341 agggttggaa attacatgta aaaggacgtt cttctaacga ggataaggat tatttagttg
    26401 aggaaatagt aggtttagaa atcaacgcta tgcttcctag cacaacagct attagccatg
    26461 cctcttttca ggaaaaggag gcaggtgatg gtagtacgca gcacatagcg gtttcaacag
    26521 agtcagttgt tgatgatgag catctacagt tggacgatga ggaaacagcc aatatagaca
    26581 cagacacacg agtcatagag gctgagccga catggataag ttttagtaga cctagtcgaa
    26641 ttgaaaaatc tcgcagggca agaaaaagta gccaaactat tttagaaaaa gaagaagcaa
    26701 caacaagtga aaatagtaat ttggttagta ctgatgagcc acacttaggt ggtgtcctag
    26761 cagcggcaga tgtgggtggg aagcaggatg caaccaatta caactctatt tttgctaatc
    26821 gatttgctgc ttttgatgag ctactttcaa ttctaaaaac taaatttgca tgtcgggtgc
    26881 tttttgaaga aaccttggtt ttgccaaaag ttgggcgtag ccgattacat ctgtgtaaag
    26941 atggctcacc aagagtgatt aaagccgttg gggtgcaacg taatggcagt gaatttgtat
    27001 tgctagaggt ggatgcatcg gatggggtga aaatgctttc taccaaagtg ttgagtggcg
    27061 ttgatagcga aacatggcgg aatgattttg aaaagatacg gcgtggagtg gtgaagagct
    27121 cattgaattg gccaaatagt ttgtttgatc aattatatgg acaagacggg catagagggg
    27181 tgaatcatcc aaaggggttg ggggagctgc aagtatcgag agaggatatg gaagggtggg
    27241 ctgagagagt ggttagagag caatttacgc attaaaggaa tgactgaaag agcctgtaaa
    27301 cccttttgtg taagtgcttt tgccggtcag ttaaaggtgg ccatttaaac ggtcaccaaa
    27361 ttcgatcata aaacggttca tggccggctt ccagttgcgg atcggcatcg tccatttctt
    27421 ggtcgccgcc tggatagcca ggtacaccac cttcatcgct gattcgtccg tagggaacac
    27481 cttgagttca tattaatgga attttctaca aatagcctcc gtggttttga ggggggatta
    27541 cagacgatcc atagtagtaa tccaatgagt tcttgagcgc ggcgacatgt ttggacgcct
    27601 tggcaaaaat tagagcctgc ttgaagtgca ggcgagcacg tgcttggacg attgatccat
    27661 tcgcggtcaa aaactcaatc ttggatgaca gcgtgtcagg aaatccaata tcgaagtccc
    27721 gccccgtgtc aaagtagggc atttcatgat caaaggacga agcttcccag gccacttccc
    27781 taatattcca gccgctagaa acaagatttc gtacgacttc atcaactttt ttgctttcca
    27841 acttgtcaca aagtgcatag gcaagagctt tggcggcaga cacgtgcgtt tcctcttaaa
    27901 cataggtcga acggataccc gaggcaaagc ggagcattag tcccaaagca tctccggtag
    27961 atccggaggc tccatactga aggcatggca tcttgggcat tgatatttga aacttctctc
    28021 tagatagcag ggatttcttg gccttgcctc atgctgatag cttgtaaata tcacacgatc
    28081 cttagcccca caaagtgagc atactccgta gctttcattt atagaaatcg tcgttagatt
    28141 gtcgcagcat gtgcacgttc cgactatttg ctcaggaagc atc
//
LOCUS       DAZSIO010000038        27601 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZSIO010000038 DAZSIO010000000
VERSION     DAZSIO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.038"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="ACHX5T_001486"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="ACHX5T_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR8466741.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="ACHX5T_001487"
     CDS             complement(1614..1937)
                     /locus_tag="ACHX5T_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR8466742.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACHX5T_001488"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACHX5T_001488"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR8466743.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACHX5T_001489"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACHX5T_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR8466744.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACHX5T_001490"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACHX5T_001490"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR8466745.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACHX5T_001491"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACHX5T_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR8466746.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="ACHX5T_001492"
     CDS             7537..8574
                     /locus_tag="ACHX5T_001492"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR8466747.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVLDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACHX5T_001493"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACHX5T_001493"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR8466748.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="ACHX5T_001494"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="ACHX5T_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR8466749.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACHX5T_001495"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACHX5T_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR8466750.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="ACHX5T_001496"
     CDS             10745..11626
                     /locus_tag="ACHX5T_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8466751.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACHX5T_001497"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACHX5T_001497"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR8466752.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="ACHX5T_001498"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="ACHX5T_001498"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR8466753.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACHX5T_001499"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACHX5T_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="ACHX5T_001500"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="ACHX5T_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR8466754.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="ACHX5T_001501"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="ACHX5T_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR8466755.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="ACHX5T_001502"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="ACHX5T_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR8466756.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="ACHX5T_001503"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="ACHX5T_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR8466757.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="ACHX5T_001504"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="ACHX5T_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR8466758.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="ACHX5T_001505"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="ACHX5T_001505"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR8466759.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="ACHX5T_001506"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="ACHX5T_001506"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR8466760.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="ACHX5T_001507"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="ACHX5T_001507"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR8466761.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="ACHX5T_001508"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="ACHX5T_001508"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR8466762.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="ACHX5T_001509"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="ACHX5T_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR8466763.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACHX5T_001510"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACHX5T_001510"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR8466764.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /gene="acpS"
                     /locus_tag="ACHX5T_001511"
     CDS             24893..25273
                     /gene="acpS"
                     /locus_tag="ACHX5T_001511"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR8466765.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="ACHX5T_001512"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="ACHX5T_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR8466766.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="ACHX5T_001513"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="ACHX5T_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFR8466767.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27538
                     /locus_tag="ACHX5T_001514"
                     /pseudo
     CDS             26442..27538
                     /locus_tag="ACHX5T_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6806 a         6866 c         7237 g         6692 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 ctggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcggcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagccactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaaaatg
    27541 aggtgtactg gcaatagcgg acactaccat ttgttctttt tttaagcagc catctgatga
    27601 t
//
LOCUS       DAZSIO010000039        27355 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZSIO010000039 DAZSIO010000000
VERSION     DAZSIO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.039"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..575
                     /locus_tag="ACHX5T_001515"
     CDS             <1..575
                     /locus_tag="ACHX5T_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8466768.1"
                     /translation="AEEDPASATRPFRTEVKRSRLLIDEYLLIRKIADVQNEWFGLCM
                     DLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPTTISRLNLTLADVLDN
                     LKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFHEIRSLSGRLHSAEKG
                     SDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            811..1629
                     /gene="ybhA"
                     /locus_tag="ACHX5T_001516"
     CDS             811..1629
                     /gene="ybhA"
                     /locus_tag="ACHX5T_001516"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFR8466769.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1630..2688)
                     /gene="modC"
                     /locus_tag="ACHX5T_001517"
     CDS             complement(1630..2688)
                     /gene="modC"
                     /locus_tag="ACHX5T_001517"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR8466770.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2691..3380)
                     /gene="modB"
                     /locus_tag="ACHX5T_001518"
     CDS             complement(2691..3380)
                     /gene="modB"
                     /locus_tag="ACHX5T_001518"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR8466771.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3380..4153)
                     /gene="modA"
                     /locus_tag="ACHX5T_001519"
     CDS             complement(3380..4153)
                     /gene="modA"
                     /locus_tag="ACHX5T_001519"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR8466772.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4320..4469)
                     /gene="acrZ"
                     /locus_tag="ACHX5T_001520"
     CDS             complement(4320..4469)
                     /gene="acrZ"
                     /locus_tag="ACHX5T_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR8466773.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4598..5386
                     /gene="modE"
                     /locus_tag="ACHX5T_001521"
     CDS             4598..5386
                     /gene="modE"
                     /locus_tag="ACHX5T_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR8466774.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5454..6926
                     /gene="modF"
                     /locus_tag="ACHX5T_001522"
     CDS             5454..6926
                     /gene="modF"
                     /locus_tag="ACHX5T_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR8466775.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7187..8203
                     /gene="galE"
                     /locus_tag="ACHX5T_001523"
     CDS             7187..8203
                     /gene="galE"
                     /locus_tag="ACHX5T_001523"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR8466776.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8213..9259
                     /gene="galT"
                     /locus_tag="ACHX5T_001524"
     CDS             8213..9259
                     /gene="galT"
                     /locus_tag="ACHX5T_001524"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR8466777.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9263..10411
                     /gene="galK"
                     /locus_tag="ACHX5T_001525"
     CDS             9263..10411
                     /gene="galK"
                     /locus_tag="ACHX5T_001525"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR8466778.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10405..11445
                     /gene="galM"
                     /locus_tag="ACHX5T_001526"
     CDS             10405..11445
                     /gene="galM"
                     /locus_tag="ACHX5T_001526"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR8466779.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11648..12400
                     /gene="gpmA"
                     /locus_tag="ACHX5T_001527"
     CDS             11648..12400
                     /gene="gpmA"
                     /locus_tag="ACHX5T_001527"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR8466780.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12567..13619)
                     /gene="aroG"
                     /locus_tag="ACHX5T_001528"
     CDS             complement(12567..13619)
                     /gene="aroG"
                     /locus_tag="ACHX5T_001528"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR8466781.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13935..14315
                     /locus_tag="ACHX5T_001529"
     CDS             13935..14315
                     /locus_tag="ACHX5T_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR8466782.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14429..15370
                     /gene="zitB"
                     /locus_tag="ACHX5T_001530"
     CDS             14429..15370
                     /gene="zitB"
                     /locus_tag="ACHX5T_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR8466783.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15367..16086)
                     /gene="pnuC"
                     /locus_tag="ACHX5T_001531"
     CDS             complement(15367..16086)
                     /gene="pnuC"
                     /locus_tag="ACHX5T_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR8466784.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16124..17167)
                     /gene="nadA"
                     /locus_tag="ACHX5T_001532"
     CDS             complement(16124..17167)
                     /gene="nadA"
                     /locus_tag="ACHX5T_001532"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR8466785.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17416..17491)
                     /locus_tag="ACHX5T_001533"
     tRNA            complement(17416..17491)
                     /locus_tag="ACHX5T_001533"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17456..17458),aa:Lys,seq:ttt)
     gene            complement(17495..17570)
                     /locus_tag="ACHX5T_001534"
     tRNA            complement(17495..17570)
                     /locus_tag="ACHX5T_001534"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17535..17537),aa:Val,seq:tac)
     gene            complement(17622..17697)
                     /locus_tag="ACHX5T_001535"
     tRNA            complement(17622..17697)
                     /locus_tag="ACHX5T_001535"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17662..17664),aa:Lys,seq:ttt)
     gene            complement(17700..17775)
                     /locus_tag="ACHX5T_001536"
     tRNA            complement(17700..17775)
                     /locus_tag="ACHX5T_001536"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17740..17742),aa:Val,seq:tac)
     gene            complement(17911..17986)
                     /locus_tag="ACHX5T_001537"
     tRNA            complement(17911..17986)
                     /locus_tag="ACHX5T_001537"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17951..17953),aa:Lys,seq:ttt)
     gene            complement(18151..18942)
                     /gene="cpoB"
                     /locus_tag="ACHX5T_001538"
     CDS             complement(18151..18942)
                     /gene="cpoB"
                     /locus_tag="ACHX5T_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR8466786.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18952..19473)
                     /gene="pal"
                     /locus_tag="ACHX5T_001539"
     CDS             complement(18952..19473)
                     /gene="pal"
                     /locus_tag="ACHX5T_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR8466787.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19508..20800)
                     /gene="tolB"
                     /locus_tag="ACHX5T_001540"
     CDS             complement(19508..20800)
                     /gene="tolB"
                     /locus_tag="ACHX5T_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR8466788.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20933..22174)
                     /gene="tolA"
                     /locus_tag="ACHX5T_001541"
     CDS             complement(20933..22174)
                     /gene="tolA"
                     /locus_tag="ACHX5T_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR8466789.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22239..22667)
                     /gene="tolR"
                     /locus_tag="ACHX5T_001542"
     CDS             complement(22239..22667)
                     /gene="tolR"
                     /locus_tag="ACHX5T_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR8466790.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22671..23363)
                     /gene="tolQ"
                     /locus_tag="ACHX5T_001543"
     CDS             complement(22671..23363)
                     /gene="tolQ"
                     /locus_tag="ACHX5T_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR8466791.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23360..23764)
                     /gene="ybgC"
                     /locus_tag="ACHX5T_001544"
     CDS             complement(23360..23764)
                     /gene="ybgC"
                     /locus_tag="ACHX5T_001544"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR8466792.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23914..24207)
                     /gene="ybgE"
                     /locus_tag="ACHX5T_001545"
     CDS             complement(23914..24207)
                     /gene="ybgE"
                     /locus_tag="ACHX5T_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR8466793.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24207..24320)
                     /gene="cydX"
                     /locus_tag="ACHX5T_001546"
     CDS             complement(24207..24320)
                     /gene="cydX"
                     /locus_tag="ACHX5T_001546"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR8466794.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24335..25474)
                     /gene="cydB"
                     /locus_tag="ACHX5T_001547"
     CDS             complement(24335..25474)
                     /gene="cydB"
                     /locus_tag="ACHX5T_001547"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR8466795.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25490..27058)
                     /gene="cydA"
                     /locus_tag="ACHX5T_001548"
     CDS             complement(25490..27058)
                     /gene="cydA"
                     /locus_tag="ACHX5T_001548"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR8466796.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         6887 a         7252 c         6702 g         6514 t
ORIGIN      
        1 ttgccgaaga agatccagct tctgcaacca ggccattcag aactgaagtg aagcgcagtc
       61 gactgttaat tgacgagtat ctgttaatca ggaaaatagc agacgtccag aacgaatggt
      121 tcggcctttg catggatctt gcactggtaa caggacagcg caaaggagat ctggcggcta
      181 tgagatggga ggatataaga gatagcaggc tatacgtgga acaacaaaaa acaggggcaa
      241 aaattcgcat ttctctcccc accacgattt ccagattgaa tttaacactt gccgatgtac
      301 tggataactt gaaaaaaata aacggtaaaa acgaaaagtt actcggaggg aagacagcca
      361 ggacaattgc agcacagttc aggattgccc gagacacatc cggattgaaa tgggaaggtg
      421 acccaccgcc atttcatgaa atccgaagtt tatccggtcg cctgcactca gcagaaaaag
      481 gcagtgattt cactcaggcg ctccttggac atcgttcatc cagcatgacg gataaatacc
      541 gtgacggacg agggagggaa tggaaagaca tttagaatta atttcgaatg atttcgaaat
      601 gccacttgta attaattgat tataaagaat taattcctaa agagcatttg cttcatgaat
      661 gctcctttgc atttagcaac tgactgtaac agctaaaatt agtcgctttt ggcggtgaat
      721 gctgaattaa aacgcagaat tttgtccgat agtggtatcg gtgtaccatt cgcgcagacg
      781 aaaattctta accttaatct ggaatacgcc atgaccacac gcgtgattgc tctcgactta
      841 gacggcacct tattgacccc gaaaaagacc ttgcttccat catcgataga agccctggcc
      901 cgcgctcgcg aagcaggcta tcaattaatc atcgtcacag gtcgccatca cgtcgctatt
      961 catccttttt atcaggcgct ggcgctggat acacctgcta tttgctgtaa tggcacctat
     1021 ttgtatgatt atcatgcaaa aaccgtgctg gaagcggacc caatgcccgt taataaagcc
     1081 ctgcaactca ttgagatgct gaatgaacac cacattcacg gtctgatgta tgtcgatgat
     1141 gcaatggtct atgagcaccc gaccgggcat gtcattcgca cgtctaactg ggcgcaaacc
     1201 ctgccgccgg aacagcgtcc gactttcaca caagtcgctt ctctggctga aacgacgcaa
     1261 caagttaacg ccgtatggaa gttcgccctg acgcacgatg acctgccgca attgcagcat
     1321 tttggtaagc atgtcgaaca tgaactggga ctggagtgtg aatggtcctg gcacgatcag
     1381 gttgatattg cacacggcgg caacagcaaa ggtaaacgtt tgacgaaatg ggttgaggcg
     1441 caaggttggt cgatggaaaa cgtcgtggca ttcggcgata actttaatga tatcagtatg
     1501 ctggaagccg ctggtacagg cgtggcgatg ggcaacgccg atgacgcggt aaaagcgcgc
     1561 gccaacattg tgattggtga taacaccacc gacagcattg cccagttcat ttatagccac
     1621 ctgatttaat caggcggtta tcgacacact tttaatttgc gcgtacagcc acaggccagg
     1681 tttgatcgcc agttcatccc tggcccacgg gctgatacgc gcccacagcg ttttaccgcc
     1741 gacttccagc tccacttcca cctggccgtt gtcgtcataa ctattaacaa cttttgcccg
     1801 caatacgtta cgaatgctgg tttgctgcgg cggttgtaaa accagagaaa catcggaagc
     1861 ctgaatgcgg atacgtagcg cagcttgcag cggttcgccc agcttattga cccacaaatg
     1921 ctgatcgccc agcgccagcg cggtcatcgc gtaatgcgga tgatgctcaa gcaccgtcac
     1981 tttcagaatg ctactttgtt gctctttcgg cagccacgga ttcatcacgc tactgcccca
     2041 cacttcctcc agcgcgccaa aggctttcac ctgaccgttt tccagtacca tcactctgtc
     2101 tgccagatgg aggatctcat ccagcgaatg gctgacatac aacatcggaa tgttgatttc
     2161 ccgtgtcagc cgttgcagat aaggcaacag ttcgcgttta cgcggaatat ccagtgacgc
     2221 cagcggttca tccagcaaca gcaattccgg tgctgtcagc aaagcccgac caatcgccac
     2281 gcgctgtttt tcgcctccgg acaggctgcc tggtaaacgg tcaagcaacg gttcaatgcc
     2341 taaaagcgcc accagcttat cgaactgatc gaccatactt ttcgacatgc cgtagcgcag
     2401 attgccacgc actttgtaat gcgggaacaa ccgcgcatcc tgaaaaacat agccaacacg
     2461 acgcttttcc ggcgtcaggc agataccttt ttcggcatca tttagtaccc gcccattgag
     2521 gacaatccgc cctttttgcg ggcgcgtcag tccactgatg gcgttaatca gcgaagtttt
     2581 tccggcaccg gagacgccaa agatagcagt gatgccattg gcgggcagcg tttcattaat
     2641 ggtcaggcaa tggttgccca acgtctggga aaaattcagc tccagcatga ttagcgcccc
     2701 gcccgttcac ggctgattct ggccagccat tctgaaatca acagggagat catcgccagc
     2761 gcaatagaaa taatgcacag tctcgccgcg ccactttccc cgccgggggt ctggatcagg
     2821 gtatacatgg cagaaggaat ggttcgcgtt tcgctaggaa tgttcgacac aaaggtgatg
     2881 gttgcaccaa actcaccgag agaacgagca aaagccagta ccgtaccaac aataattccc
     2941 ggtaaggtca gcggtaacgt gatagtaaag aaaacgcgcc agcgcccggc acctagtgtt
     3001 cttgcggcct gttccagttt gacatcaacc ccttccagcg ccagacgaat tgcccgcacc
     3061 atcagcggaa acgacatgac ggcggcagcg agaaccgcgc cgcgccagct aaaggcgaag
     3121 gtaataccaa accagtcata cagacgttca ccgataaatc cgcgccgtcc catcgaaact
     3181 aataataagt aaccgacgac cacgggcggt aacaccagcg gtagatgcag tacgctgtcg
     3241 agcagagctt tgcccggaaa cgtgcaacgc accagtaacc aggcaaaaaa gatcccaaac
     3301 ggcaggctaa acagcacagc cagggaagaa actttcaggc ttaataaaac tgcctgccat
     3361 tctggatcgg tcagtatcat tacttggttg taaatccgta acgtttaaag atttccgctg
     3421 cctgcggtcc cttcagataa tcataaaatg ctttcactgt cgcattgtta tgcccttcca
     3481 caacagcaac cggatattcc acttttttat gtgaatcttc cgggaaggtg gcaaccactt
     3541 ttaccccttt gctggcaact gcgtcagaac cgtagacaat gcccagaggc gcttcgttac
     3601 gttcgaccag cgccagcgcc ccacgaacat cttccgctgg ggccagtttc ggagagagcg
     3661 tatcccatgc gcccagtttt tgcagtgctt cttttgcata aatgccagcg ggaacatgtt
     3721 ccggatcgcc aaccgccagg cgaccgccat tcagcagcga agtccagtta gttttgctgt
     3781 cgatggtgaa atctttctgc tcgctggctt tcggtgctac aacgaccagg ctattgccga
     3841 gcagtgtctg acgcgtagca gtatcgatcg cttttttatc aaccgcataa tccatccatt
     3901 tctgatcggc agaaataaac agatccgcag gggcacccgc ttcaatctga cgggcgagag
     3961 tagatgacga agcgaaagaa gaaaccacat ccacgccttt ctctttttta tactgcgtag
     4021 caatgtcctg cattgcgtta gtcagtgatg cggcggcgaa caccgtgatt ttcccttcat
     4081 ctgccagtgc attgccagca actgcgaaag agagtgccgc cccggcaaac aagttcaacc
     4141 atttacgagc cattggtaac cccttaatgt aacgttatgt aggcgacaat ataacgacta
     4201 actcggaatt ttcccagcag ttattgctaa cctttgtgag gtagataaga aaaaatatcg
     4261 gcaggaaaag caggaagttg agaaaaagaa aatgcccgac taagcgggca ctcagggaat
     4321 caatgatttt gtccgggctg gtctttttta ccaacaccag aaaagatgtt gaatacttca
     4381 ccaagaccgt aaatcagacc caggatgatg gccatcacga caggtaccat gattacggcg
     4441 aataccagac tttttaataa ctctaacatg gtcaactcca gatatagtca tgagactatt
     4501 ctaaccgcta agcacagaaa agcactcccc ttttgtgcgg tcagctttgc gtggcgttcg
     4561 ttttccgtca caataagact tttgccagga cattgttatg caggccgaaa tccttctcac
     4621 ccttaagctc caacaaaaat tattcgccga cccgcgccgc atttcgctac taaaacacat
     4681 tgcgctttcc ggttccatta gccagggagc gaaagatgcc ggtatcagct ataaaagcgc
     4741 ctgggatgcc attaacgaga tgaatcagtt aagtgagcat attctggtcg agcgcgcaac
     4801 aggcggtaaa ggtggcggag gcgcagtact gacccgctat ggtcagcgac tgattcagct
     4861 ctatgactta ctggcgcaaa tccagcaaaa agcctttgat gtgttaagtg acgatgacgc
     4921 cctgccgctg aacagcctgc tggccgcgat ctcacgtttt tcactgcaaa ccagcgcccg
     4981 taaccagtgg ttcggtacca tcaccgcccg cgatcatgat gacgttcaac agcatgttga
     5041 tgtcttactg gctgacggaa aaacacgcct gaaagtcgca attaccgcac aaagcggcgc
     5101 acgtctgggg ctggatgaag gcaaagaagt gttgatattg ctaaaagcgc cgtgggtagg
     5161 cattactcag gacgaagcgg tcgcacaaaa cgccgacaac caattaccgg gtattattag
     5221 tcatattgag cgcggcgcag agcagtgcga agtattaatg gcgctacccg acgggcaaac
     5281 actgtgcgcc acagtgccgg taaatgaagc gacttctctt cagcaagggc agaatgtcac
     5341 ggcctacttt aatgccgaca gcgtgattat cgccacgctg tgctaagcgt gttgacaatt
     5401 tgttatgaaa cacgtatccc tgtcagtaat cgctgcacaa aatgggatat aaaatgtcat
     5461 cgttgcaaat tttgcaaggc acgtttcgtc ttagcgacac aaaaacgctg caattgcctc
     5521 agctaacgtt aaacgcgggt gatagttggg cgtttgtcgg ttcgaatgga agtggaaagt
     5581 cggcgctggc ccgcgcgctg gcgggggaac ttccgctttt gaaaggtgaa cggcaaagcc
     5641 agttttccca catcactcgt ctctccttcg agcaattgca aaagctcgtc agcgacgaat
     5701 ggcaacggaa taacaccgat atgctcggcc ctagcgaaga ttacaccgga cgcactacgg
     5761 ctgagattat tcaggatgaa gtaaaggatg caccgcgttg catgcaactg gcgcagcagt
     5821 tcggtattac cgccctcctc gaccgacgct ttaaatacct ttccactggc gagacgcgaa
     5881 aaaccctgct gtgtcaggcg ctgatgtcgg agcctgactt gttgattctt gatgagccgt
     5941 tcgatggcct ggatgttgcc tcacgtcagc agctggctga gcgactcgcc tcgttacatc
     6001 agtccggtat tactttggta ctggtgctca atcgcttcga taagatcccg gaatttgtcc
     6061 agtttgctgg cgtgctggcg gattgtacgt tagcggaaac tggcgctaaa gaggaactgc
     6121 tccagcaagc actcgtcgcg caacttgcgc atagtgaaca gcttgaaggt gtgcaactgc
     6181 cggagccgga tgaaccttca gcacgtcacg ccttacccgc caacgaatcg cgcattgtgc
     6241 tgaacaatgg cgtggtttct tataatgatc gccccattct taataacctt agctggcagg
     6301 tgaatccagg cgaacactgg caaattgtcg ggccaaatgg cgcgggaaaa tcgacgttat
     6361 taagcctgat tactggcgat catccgcaag gttacagcaa cgatttgacg cttttcggcc
     6421 gacgtcgcgg cagcagcgaa accatctggg acatcaaaaa gcatatcggt tacgtcagca
     6481 gtagtttgca tctggattac cgggtcagca ctaccgtgcg taatgtgatt ctttctggct
     6541 attttgattc gattggcatt tatcaggtcg tttcggatcg ccagcaaaaa ctggtgcagc
     6601 agtggctgga tattctcggc attgataaac acacggctga cgctccgttc catagtcttt
     6661 cctggggaca gcagcgtttg gcgctgatcg tccgcgcact ggtgaaacat ccgacgttgc
     6721 ttattctcga tgaaccacta caggggcttg atccgctcaa tcgccaactt atccgccgtt
     6781 ttgttgatgt gctgattagc gaaggtgaaa cgcaattgtt gtttgtttcg caccacgctg
     6841 aagatgcgcc tgcctgtatt acccatcgcc ttgagttcgt gccggacggt ggactctatc
     6901 gctatgtgct gacaaaaata tattgagtcg gtagtgctga ccttgccgga ggcggcctaa
     6961 gcgcactctc cggccaacgg ttcgacgcat gcaggcatga aaccgcttct tttttcagat
     7021 aaaaagcgca atcattcata aaccctctgt tttataatca cttaatcgcg cataaaaaac
     7081 ggctaaattc ttgtgtaaac gattccacta atttattcca tgtcacactt ttcgcatctt
     7141 tgttatgcta tggttatttc ataccataag cctaatggag cgaattatga gagttctggt
     7201 taccggtggt agcggttaca ttggaagtca tacctgcgtg caattactgc aaaacggtca
     7261 tgatgtcatc attcttgata acctctgtaa cagtaagcgc agcgtactgc ctgttatcga
     7321 gcgtttaggc ggcaaacatc caacgtttgt tgaaggcgat attcgtaacg aagcgttgat
     7381 gaccgagatc ctgcacgatc acgctatcga caccgtgatc cacttcgccg ggctgaaagc
     7441 cgtcgccgaa tcggtacaaa aaccgctgga atattacgac aacaatgtca acggcactct
     7501 gcgcctgatt agcgccatgc gcgccgctaa cgtcaaaaac tttattttta gctcctccgc
     7561 caccgtttat ggcgatcagc cccaaattcc atacgttgaa agcttcccga ccggcacacc
     7621 gcaaagccct tatggcaaaa gcaaattgat ggtggaacag atcctcaccg acctgcaaaa
     7681 agcccagccg gactggagca ttgccctgct gcgctacttc aacccggttg gcgcgcatcc
     7741 gtcgggcgat atgggcgaag atccgcaagg cattccgaat aacctgatgc catacatcgc
     7801 ccaagttgct gtaggccgtc gcgactcgct ggcgattttt ggtaacgatt atccgaccga
     7861 agatggtact ggcgtacgcg attacatcca cgtaatggat ctggcggacg gtcacgtcgt
     7921 ggcgatggaa aaactggcga acaagccagg cgtacacatc tacaacctcg gtgctggcgt
     7981 aggcagcagc gtgctggacg tggttaatgc cttcagcaaa gcctgcggca aaccggttaa
     8041 ctatcatttt gcaccgcgtc gcgaaggcga ccttccggcc tactgggcgg acgccagcaa
     8101 agccgaccgt gaactgaact ggcgcgtaac gcgcacactc gatgaaatgg cgcaggacac
     8161 ctggcactgg cagtcacgcc atccacaggg atatcccgat taaggaacga ccatgacgca
     8221 atttaatccc gttgatcatc cacatcgccg ctacaacccg ctcaccgggc aatggattct
     8281 ggtttcaccg caccgcgcta agcgcccctg gcagggggcg caggaaacgc cagccaaaca
     8341 ggtgttacct gcgcacgatc cagattgctt cctctgcgca ggtaatgtgc gggtgacagg
     8401 cgataaaaac cccgattaca ccgggactta cgttttcact aatgactttg cggccttgat
     8461 gtctgacacg ccagatgcgc cagaaagcaa cgatcctttg atgcgttgcc agagcgcgcg
     8521 cggtaccagc cgggtgatct gcttttcacc ggatcacagt aaaacgttgc cagaactgag
     8581 cgttgcggca ttgacggaaa tcgtcaaaac ctggcaggag caaaccgcag agctgggaaa
     8641 aacgtacccg tgggtgcagg tctttgaaaa caaaggtgcg gcgatgggct gctctaaccc
     8701 gcatccgcac ggacagattt gggcaaatag cttcctgcct aacgaagctg agtgcgaaga
     8761 ccgcctgcaa aaagaatatt ttgccgaaca gaaatcacca atgctggtgg attatgttca
     8821 gcgcgagctg gcagacggta gccgtaccgt tgtcgaaacc gaacactggt tagccgttgt
     8881 gccttactgg gctgcctggc cgttcgaaac gctactgctg cccaaagccc acgttttgcg
     8941 gatcaccgat ttgaccgacg cccagcgcag cgatttggcg ctggcgttga aaaagctgac
     9001 cagtcgttat gacaacctct tccagtgctc cttcccctac tctatgggct ggcacggcgc
     9061 accatttaat ggcgaagaga atcaacactg gcagctgcac gcgcactttt atccgcctct
     9121 gctgcgctcc gccaccgtac gtaaatttat ggttggttat gaaatgctgg cagagaccca
     9181 gcgagacctg accgcagaac aggcagcaga gcgtttgcgc gcagtcagcg atatccattt
     9241 tcgcgaatcc ggagtgtaag aaatgagtct gaaagaaaaa acacaatctc tgtttgccaa
     9301 cgcatttggc taccctgcca ctcacaccat tcaggcgcct ggccgcgtga atttgattgg
     9361 tgaacacacc gactacaacg acggtttcgt tctgccctgc gcgattgatt atcaaaccgt
     9421 gatcagctgt gcaccacgcg atgaccgtaa agttcgcgtg atggcagccg attatgaaaa
     9481 tcagctcgac gagttttccc tcaatgcgcc cattgtcgcg catgaaaact atcaatgggc
     9541 gaactacgtt cgtggcgtgg tgaaacatct gcaactgcgt aacaacagct tcggcggtgt
     9601 ggacatggtg atcagcggca atgtgccgca gggtgccggg ttaagttctt ccgcttcact
     9661 ggaagtcgcg gtcggaaccg tattgcagca gctttatcat ctgccgctgg acggcgcaca
     9721 aatcgcgctt aacggtcagg aagcagaaaa ccagtttgtt ggctgtaact gcgggatcat
     9781 ggatcagcta atttccgcac tcggcaagaa agatcatgcc ttgctgattg actgccgctc
     9841 actggggact aaagcagttt ccatgccgaa aggtgtggct gtcgtcatca tcaacagtaa
     9901 cttcaaacgt accctggttg gcagcgaata caacacccgt cgtgaacagt gcgaaaccgg
     9961 tgcgcgtttc ttccagcagc cagctctgcg cgatgtcacc attgaagagt tcaatgctgt
    10021 tgcacatgag ctggacccaa tcgtggcgaa acgcgtgcgt catatcctga ctgaaaacgc
    10081 ccgcaccgtt gaagctgcca gcgcgctgga gcaaggcgac ctgaaacgta tgggcgagtt
    10141 gatggccgag tctcatgcct ctatgcgcga tgatttcgaa atcaccgtgc cgcaaattga
    10201 cactctggta gaaatcgtca aagctgtgat tggcgacaaa ggtggcgtac gcatgaccgg
    10261 cggcggattt ggcggctgta tcgtcgcgct gatcccggaa gagctggtgc ctgccgtaca
    10321 gcaagctgtc gctgaacaat atgaagcaaa aacaggtatt aaagagactt tttacgtttg
    10381 taaaccatca caaggagcag gacagtgctg aacgaaactc ccgcactggc acccgatggt
    10441 cagccgtacc gactgttaac tttgcgtaac aacgcaggga tggtagtcac gctgatggac
    10501 tggggtgcga ctttactttc cgcccgtatt ccgctttccg atggcagcgt ccgcgaggcg
    10561 ctgctcggct gtgccagtcc ggaatgctat caggatcagg ctgcgtttct gggggcctct
    10621 attggtcgtt atgccaaccg catcgccaat agtcgctata cctttgacgg tgaaaccgtg
    10681 acgctttcgc caagccaggg cgttaaccag ctgcacggcg gcccggaagg gttcgacaaa
    10741 cgtcgctggc agattgtgaa ccagaacgat cgtcaggtgc tgtttgccct gagttcagat
    10801 gatggcgatc agggcttccc gggtaatctc ggcgcgacgg tgcaatatcg tctgaccgac
    10861 gataaccgta tctccattac ttatcgcgcc acagttgata aaccttgccc ggtgaatatg
    10921 actaatcacg tctatttcaa tcttgacggc gagcagtctg acgtgcgcaa tcacaagttg
    10981 cagattctgg cggacgaata tcttccggtt gatgaaggcg gcattccgca cgacggcttg
    11041 aaatctgtcg ccggaacgtc ttttgatttc cgcagcgcca aaatcatcgc cagtgagttt
    11101 cttgccgacg acgatcagcg caaagtgaaa ggttacgatc acgcattctt gttacaggcc
    11161 aaaggcgatg gcaagaaagt ggcggcgcat gtctggtcag cagatgaaaa attgcagctg
    11221 aaggtctaca ccaccgctcc ggctcttcaa ttctactccg gcaacttcct cggcggcaca
    11281 ccgtcgcggg gaaccgaacc ttacgccgac tggcaagggc tggcgttgga aagcgagttt
    11341 ctgccggaca gcccgaacca ccctgaatgg ccgcaaccgg actgcttcct gcgtcctggc
    11401 gaagagtatt ccagcctgac ggaatatcag tttattgctg agtaatgtta agccctcgtc
    11461 atgagggctt tatcttcata ttgttcaaat caccagcaaa caccgacata tttgcaactc
    11521 aatattcaca acaaccttac actgcgccac tattttcgct atggttatgc gtaagcattg
    11581 ctgttgcttc gtcacggcaa tataatgaga attattatca ttaaaagatg atttgaggag
    11641 taagcatatg gctgtaacta agctggttct ggttcgtcat ggcgaaagtc agtggaacaa
    11701 agaaaaccgt ttcaccggtt ggtacgacgt ggatctgtct gagaaaggcg taagcgaagc
    11761 aaaagcagca ggtaagctgc tgaaagagga aggttacagc tttgactttg cttacacttc
    11821 tgtgctgaaa cgcgctatcc ataccctgtg gaatgtgctg gacgaactgg atcaggcatg
    11881 gctgcccgtt gagaaatcct ggaaactgaa cgaacgtcac tacggtgcgt tgcagggtct
    11941 gaacaaagcg gaaactgctg aaaagtatgg cgacgagcag gtgaaacagt ggcgtcgtgg
    12001 ttttgcagtg actccgccgg aactgactaa agatgatgag cgttatccgg gtcacgatcc
    12061 gcgttacgcg aaactgagcg agaaagaatt gccgctgacg gaaagcctgg cgctgaccat
    12121 tgaccgcgtg atcccttact ggaatgaaac cattctgccg cgtatgaaga gcggtgagcg
    12181 cgtaatcatc gccgcacacg gtaactccct gcgtgcgctg gtgaaatatc ttgataacat
    12241 gagcgaagaa gagattcttg agctgaatat cccgactggc gtgccgctgg tgtatgagtt
    12301 cgacgagaat ttcaaaccgc tgaaacgcta ttatctgggt aatgctgacg agatcgcggc
    12361 gaaagcagca gccgttgcga accagggtaa agcgaagtaa acgtcattcg tttaaaaaat
    12421 gagaaagccg actgcaagtg agtcggcttt tttgtttgtc gttgctaaca atggagcaca
    12481 ttgcctgatg cgacgctgcg cgtcttatca ggcctgtggt gattcatcgg atacgccact
    12541 ctgacggcgc atccgacaat taaaccttac ccgcgacgcg cttttactgc attcaccagt
    12601 tgacgtaaca gagcatcggt atcttcccag ccaatgcagg catcggtgat gctcttaccg
    12661 taggccagcg gctccccgct atcgaggctc tgattgcctt ccaccagatg gctttccacc
    12721 atcacgccaa taatggcctt ttcgccaccg gcaatctgct ggcaaacgtc agcacaaaca
    12781 tccatctgct ttttgaattg tttggacgaa ttagcatggc tgaaatcgat catcacctga
    12841 gctggcagac ctgctttgtt cagcccttct ttcacttcag caacgtgctt cgcgctgtag
    12901 ttaggctctt taccgccgcg cagaatgata tggcaatcgc cgttaccact ggtattcaca
    12961 atcgccgaat gcccccattt agttacggac aggaagcagt gcggcgcacc ggcggcatta
    13021 atggcatcga tagccacttt aatcgtaccg tctgtgccat ttttaaaacc taccggacaa
    13081 gaaagaccag acgccagttc gcggtgcacc tgcgattccg tggtacgtgc accaattgcg
    13141 ccccagctca tcaggtcagc gagatactga ggagtgatca tatcgagaaa ctcacccgcc
    13201 gctggcagac cgctgtcgtt aatatcaagc agcaatttac gggctatacg cagaccgtcg
    13261 ttgatctgga agctgttatc catatgcgga tcgttaatca accctttcca gcccaccgtg
    13321 gtacgcggct tttcaaaata gacgcgcatt acgatttcca gctcatcttt cagctcttca
    13381 cgcagcgcca gcaagcgagt ggcatactct ttagccgcga caggatcatg aattgagcac
    13441 gggccaatca ccaccaacag gcgatcatca ttacctttca ggatcttatg gatcgctttt
    13501 cgggcatggg caaccgtatt cgcggcattt tcagttgcgg ggaatttttc cagcaatgcg
    13561 acaggaggaa gtaactcttt gatttctttg atgcgtaaat cgtcgttctg ataattcatg
    13621 tctgttccag tgttgccata cttatcttag tgaatgcaat acttccaatc tatatcttcc
    13681 gtcagaatgt gtaaacgggg ttttacacta tgaacggatt aatcctggaa ttagcaagaa
    13741 aaacgccaga ttgtcgcgaa aaacgagatc tctcctacaa tttctaactg taactccttt
    13801 gtttgttaat tatttcaaga ttctctgctg cgtttcataa actggctgaa atcttaaacc
    13861 aatgccttat attcacctgc aaatgcactg ttggaagagg ttatccgaca taacgaccat
    13921 aacaggagca tcctatgaaa atgacaaaac tggccacatt atttctgact gccactctaa
    13981 gccttgccag cggtgccgca ctggctgccg atagcggagc gcaaactaat aacggccagg
    14041 caaacgccgc agctgatgcg ggccaggtag cccctgacgc ccgtgaaaat gtcgcgccaa
    14101 ataacgtcga caataacggg gtaaataccg gttctggcgg cacaatgctg cattcggatg
    14161 gttcttcaat gaacaatgac ggaatgacca aagatgaaga gcacaagaac accatgtgca
    14221 aagatggtcg atgtccggat atcaataaaa aggtacaaac cggtgatggc atcaataatg
    14281 atgtcgatac caaaaccgac ggtaccacgc agtaaccctg caaaatagcc aataatggga
    14341 gagcggagtc tctccctttt aatttttcac ttctaaaagc agtatgatgt cataaagtta
    14401 agtgagaata attaagggat gactcgtcat ggcgcactca cactcacaca cgtcctcaca
    14461 cctgccagaa gataataatg ctcgtcgctt gttgtatgct ttcggcgtga ctgccgggtt
    14521 tatgctggta gaagtcgttg gtggttttct ttctggttct ctggcattgc tggccgatgc
    14581 gggtcatatg ttgaccgata ctgccgccct gcttttcgcc ctactcgccg tgcaattttc
    14641 acgtcgtcct cccaccattc gccacacttt cggttggcta agactgacca cgctcgcggc
    14701 gtttgtgaat gccatcgccc tcgtggtaat taccattttg attgtctggg aggcgataga
    14761 acgcttccgc acgccgcgtc cggtcgaggg aggcatgatg atggcaattg ccgtggctgg
    14821 gttgctggca aatatacttt ctttctggtt acttcatcac ggcagcgaag agaaaaacct
    14881 caacgtacga gcagcggcac tgcatgtgct cggggacctg ctgggttcgg ttggggctat
    14941 tattgccgcg ttgattatta tctggactgg ctggacacct gctgacccca ttctctcgat
    15001 actggtgtcg cttctggttc tgcgtagcgc gtggcgactg ttgaaagata gcgtgaatga
    15061 attacttgaa ggtgcaccgg tatcgctgga tatcgctgaa ctgaagcgtc gtatgtgccg
    15121 ggaaatcccg gaagtccgca atgtgcacca tgtacatgta tggatggtag gcgagaagcc
    15181 ggtgatgacg ttgcatgtgc aggtgatccc gccacacgat cacgatgcct tgttggatca
    15241 gatccaacac tacctgatgg atcactatca gattgagcac accaccattc agatggaata
    15301 tcaaccttgt cataggccag actgccacct gaatgaaggg gtgtctggcc attcacatca
    15361 tcaccattaa tgggacagcg cgcgtgagcc tctttcacgt gcgctgttga tccacatccg
    15421 gctgccgttg agcgcaataa aggtcaggat gatgtactcc agagacatgg cgtaaacgcc
    15481 ctgaagtgca aaaataacga cgctaatcac gttaataatc acccacaaca gccagttttc
    15541 cacatactta cgcgtcatca gaatcattgc cacgatagat aacaccatca tgcatgaatc
    15601 ccagaacggg aaagcgtccg gttgcagttc aggcatcaca acctgtaatc ctaatgcttg
    15661 catgatcatg actgccacgc gggtcaaaaa tgcaaacacc ggattgataa ataccgtcat
    15721 cagaccaatc gaaacaacgc aaaccgccaa ccagctgagt gccttcggca atggcaacca
    15781 gcgaattttc aactccgcct cgttctgact ggtttgtcgc gaccacgcat accagccgta
    15841 aatattcgcg gcaaagaaaa acacctgtaa tagcaggctg gcatacagct gaatctgaaa
    15901 gaaaataatg ccaaacaagg taacgttgat caggccaaag aagtagttgc tgatcttctc
    15961 cagactggca aggccaatac acagcaaccc ggcgatcgtg cctaccgctt cgatccatga
    16021 gagatcataa ccgcccgccc ctattggtat atgtaccagg atattctgca cactaaaaaa
    16081 atccatcttt cccccaaagc gtaacgttac gccttattat tcgttatcca cgtagtgtag
    16141 ccgcaaaatc cagcatacga ttgagcggca ccagcgccct ctctcgcagc ctctcatcga
    16201 catgaacctc gtgattactt ccttcctgtt ctaatgcctc tgcgatagcc tgaaggtcat
    16261 tcatggccat ccacggacaa tgcgcgcagc tgcggcaggt tgcgccctca cccgcggttg
    16321 gtgcttccag taactcttta tctggcaccg cctgctgcat tttgtagaaa ataccccgat
    16381 cggttgccac aataagcctc tgatgaggca atgcttttgc agcagcgatc agttgactgg
    16441 tggaaccgac cgcatccgcc atatcgacaa tagcttgtgg tgattctgga tgcaccagta
    16501 tggcagcatc cgggtattct tcttgcaagc gggttaacgc ctgagtctta aattcatcat
    16561 gcacaatgca ggcaccctgc cagcataaaa tgtctccacc cgtctgtttt tgcacgtaac
    16621 gccccagatg tttgtcgggt gcccagatga ttttttcacc caaactatca agatgatcaa
    16681 taagttcgac ggcaatgctt gaagttacca cccaatctgc gcgcgctttt accgcagcag
    16741 aagtgttggc gtagacgacg acagtacgat cgggatgggc atcgtaaaat gcgttaaatt
    16801 cttcaacagg gcagccgaga tccagtgaac actcaacctg aagtgtcggc atcagaattg
    16861 ttttttccgg actgagaatt ttggcggttt ctcccataaa tctcacccca gcgactaaca
    16921 aagtagaagc gggatgcttt gcaccgaagc gcgccatttc cagagaatca gaaatacagc
    16981 caccggtttc ttctgccagt tgttgaattt cgggatcggt atagtagtgg gcaaccatca
    17041 ccgcattacg ttcttttagc agacgtttta tcttctcgcg gtaatacgct ttttcatcaa
    17101 tgcttaacgg cgtcggcttc ggggggaaag gataaatcgc cgtgtctgga tcaaacatta
    17161 cgctcatctt accatctcgt tttacaggct taacgttaaa accgacatta gcgtaacatt
    17221 cgctgttatc ggtgtaattt gttttatatg ctaaacaaga tagtcgaatg gtggggaaaa
    17281 gtcactcgaa ttacgcctaa ttccaccgac gtagagttgc ttgcacagta agctctgacg
    17341 atgtcatcag gtttacaaag tgaaattgag gatgcctgaa tagcaaaaaa gcgcctttag
    17401 ggcgcttttt tgcattggtg ggtcgtgcag gattcgaacc tgcgaccaat tgattaaaag
    17461 tcaactgctc taccaactga gctaacgacc cgagtggtgg gtgatgacgg gatcgaaccg
    17521 ccgaccccct ccttgtaagg gaggtgctct cccagctgag ctaatcaccc gatactgcgc
    17581 tggatactac aaactacttc caccgaaccc accgtaatta gtggtgggtc gtgcaggatt
    17641 cgaacctgcg accaattgat taaaagtcaa ctgctctacc aactgagcta acgacccggt
    17701 ggtgggtgat gacgggatcg aaccgccgac cccctccttg taagggaggt gctctcccag
    17761 ctgagctaat cacccgacac tacgctggat actacaacta actatgttag tgggggtcgt
    17821 gcaggatgac tcgacttcgt ctcgcccttc ggcgccgttg ctgatgcaac gttatccttc
    17881 acgttttact accgcttcca ccttagcgat tggtgggtcg tgcaggattc gaacctgcga
    17941 ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccactt tttcgttgct
    18001 ttcggtttgt ttgatatccc gtggcaacgg cggcatatat tactgatttc agacttgagc
    18061 gcaacaaaaa tttcgatgca gatcactcaa ctgcttatga ttcgcacgac acgaccagaa
    18121 ataatgcgac ttctggtcgt gtgttatgca ttacatcgcg ttcagacgtt tttgtgcctg
    18181 tttagcgcca tcagtaccag ggtatttact gataacctgc tggtatacgg ctttcgcttt
    18241 tgcggtgtca cctttgtcct gcatgatgac gccgacttta aacatcgcat cagcagcctt
    18301 tggtgacttc ggatagtttt tcactaccga agcaaaatag tacgccgcat catctttttt
    18361 acccttgttg tagtttaact gacccagcca ataattggcg tttggcaggt aagttgaatc
    18421 agggtaattt ttgatgaaat tctgaaatgc caccattgcg tcatcctggc gggatttatc
    18481 ctgcaccagc gcaatagctg cattgtaatc cgtgtttgca tcgccgcttt tcaccggcgc
    18541 gccagcattc gcagtaccag catcagctgt cggcgtcgtt gatgccgtcg caccgctttg
    18601 atcgctgctg gttgattgcg ccgctgcacc accgctgctg aggctgtcga tctgcaacag
    18661 gatctgcttt tgccgctcca cgacctgatt cagttgatac tgattttcct gaatctgacc
    18721 acgcagggaa tcaatatcgg attgattatc agaaagttgt tgctggagtt gggttaaaag
    18781 ctggctgtga gcattagaaa tacgctcaag ttgagtgacg cggtcttcga ccgagcctga
    18841 gctgacacta ctgattggtg cctgagcaaa agcggcccag ggggccgcta taccaaccag
    18901 taacgacaga ctcaatagtt gatgtctgaa gttactgctc atgcaattct cttagtaaac
    18961 cagtaccgca cgacggtttt tggagtatgc cgcttcgtca tgacccagta ctgcaggttt
    19021 ttctttaccg taagaaacga tggagatctg gtctgcagaa acgcctttac cctgcaggta
    19081 catcttaacg gcgttcgcac gacgttcacc cagggagatg ttgtattccg gagtaccacg
    19141 ttcgtccgcg tgaccttcta cggtgacttt gtaagacggg ttgctacgca ggaagtttgc
    19201 atgtgcatcc agcatttgag cgaagtcaga acggatatcg tacttgtcca gatcgaagta
    19261 aacgatgttg ttctgctgca gctgttgcat ttgcagacga gcctgctctt cggaagacat
    19321 gttgccgttg ccgccgttcg catccatacc agtgccggca cccagcatgc cttcgctgcc
    19381 gtcattgctg gcgttcttgt tggaagaaca tgccgcaatt gccataacag gcagagcaat
    19441 catcagccct ttcagcactt tgttcagttg catttcaatg attcctttac tattcaatta
    19501 attattatca cagatacggc gaccaggcag ggaatttgac ctgtccatca gttgccggaa
    19561 gacgcgcttt gaaacgccca tctgtagaaa ccaaattcag cacggatccc atcccctgag
    19621 aagagctgta gattaccata gtgccgttag gtgccagact tggcgtttca tccaggaacg
    19681 tggacgacag aacttgtacg cctcccgttg ccagatcttg tttggcaatg tgctgctgcc
    19741 caccattgga gctgaccatt accataaatt taccgtcgct gctgacatcc gcatcctggt
    19801 tctgcgaacc ttcccaggta atacgttgtg gcgcaccgcc gttgatattc actttataaa
    19861 cctgcggacg accggcctgg tcagaagtaa atgccaggtt ctggctatcc gggaaccagg
    19921 tcggttcggt attgttactg cgaccatcag tcacctggcg gatctgacca gaagccaaat
    19981 ccattacgta caggttcaga ctaccggttt tcgacaaggc gaatgccagt ttgctgccgt
    20041 ctggcgagaa tgcaggtgca ccgttgtgac gcgggaatga agccacctga cgtacagcgc
    20101 catttgccag cgtctgaata accagcgcgg aacgaccgct ttcgaaggtc acataagcca
    20161 gtttagaacc gtctggtgac cacgccggag acatcagcgg ctgcggtgaa cggtgaacga
    20221 caaactggtt gtaaccgtca tagtcagata cgcgcagttc atacgggaac tgaccgccgt
    20281 tggtctgaac aacgtaggca atacgggtac ggaacgcacc tttaatgccg gtcagttttt
    20341 caaacacttc atcactggcg gtatgaccag cataacgcag ccactgcttg ttcactttgt
    20401 acgagttctg agcaagtaca gtacccggtg cgccgccagt gtcaacaagt tgataagcaa
    20461 cattgtaaga accatccgga ttcggagtga cttgaccgac aactacagcg tcaatgccca
    20521 gtgcggacca tgcagctggt tgtacttcct gcgcactacc cggctgctgt ggcagacgag
    20581 cgcgatctaa cggattaaat ttaccgctgt tacgcaagtc agcagcaacg atgccgccaa
    20641 tatcttcagg tgccgcacca ggccccgccc actggaaagg aacaacacca ataggacgac
    20701 cggaatctac accgctgtcg atcacaatgc ggacttcagc atgcagaact gatgcccaca
    20761 gtatgagaaa accaaatgct actcgtaatg cctgcttcat catatctccc ttatctggac
    20821 cgatggccca cgataattta gcagaatgtt aacaaactaa aatacacaaa actaccagaa
    20881 ccccgtggca accggtgcct gatgttgacc gtccgaacag tcaacatcgc gattacggtt
    20941 tgaagtccaa tggcgcgttt ttgaacactt catatactgc ctggcttggt ggtttcggga
    21001 tcttcgcaag tttagctgct gccaacgcag cctgacaaag tgcgggatcg ccaccttcag
    21061 gtttgatatc cagtaacata ccatcgggtg ccagttttat gcgcagcgta caggttttgc
    21121 ctgcatagga cgatgcgtca tagaacttac tttcgatagc agatttaatc tgcccggcat
    21181 agttattgat atcggcccct gatgcgccat tgtttttagt attaccactc ccggcaggcg
    21241 aagcattgtt ccctttcgcc cctccccccg ttttcggtgc attcttacca gagcttagct
    21301 caccgaaaat atcatctgcc tctgcggcag cttttgctgc agcggctttt tcagctgctg
    21361 cttttgccgc tgccgctttt ttgtctgcgg cggctttttc tgccgctgct ttcttatcag
    21421 ctgctgcctt atcagcagcc gctttttctg ctgctttttt gtcggctgca gctttctctg
    21481 ctgccgcttt cttatcagct gcagcctttt cagcagccgc tttcttctca gcttctgcct
    21541 tggctttttc agcagcttca gttgccgctt tctttcttgc ttcagctgca gctgcttctg
    21601 ccgcttccgc tttcttcttc agtgccgcag cggctacctc ggcttttttc tgcgcttcgg
    21661 ctgcggcttt ggcggcttct gcttctgctt ttttcttcgc gtctgcagcc gctttcttcg
    21721 ctgcttcttc cgcagcttta gcatctgccg ccgctttcgc tgccgccact tcagcttgct
    21781 tctgctttaa ctcggcctgt tttgcggctt cttcagcctg ctttttctgc tcctgagccg
    21841 ctaaccgctc tttctcaagt tgcttcaggc gttcctgttc agccgcttgt ttctcacgca
    21901 gttcttcagc agcctgctgt tccttcatct tgcgctgttc atcagaacgc ttcgcgcttg
    21961 attcctggct ttgcatgcgt ttgtactgct caactaccgc acctgaatca accatgacag
    22021 cgtcgatgga cgaaccaccg ccgcctccgg ctgaagcttc tatattctca tcgaacgaac
    22081 tccagatcag cgccgcaaat aagatgacat gcagcactgc tgaaataatt atcgcccgct
    22141 tgagcttgtc gttttgttcg gttgcctttg acactctcgg tttccaaaaa ctgttcgcct
    22201 gttacccgct ctctttcaag caagggaaac gcagatgttt agataggctg cgtcattaaa
    22261 ccaaccgatt tcacacccgc actatgtaac aagttcagtg ctttaattat ttcatcgtaa
    22321 ggcacatctt ttgcgccacc gatcagaaag accgttttcg ggttggcctt gaaacggctg
    22381 gacacttccg ccaccacctg ctctggtggt aaacgctcca ggcgatcttt ctcaaccacc
    22441 acggtgtact gaccaatacc agacacttca acaatcactg gcggattatc gttactgctc
    22501 accgcctgtg attcagtagc gtctggcaga tcgacctcca cgctctgggt gatgatgggc
    22561 gctgtcgcca taaagatcag caacagcacc agcagtacgt ccagcaacgg aacaatgttg
    22621 atttcggact tgagatcgcg acgacctcgt ccacgcgctc tggccatggc ttaccccttg
    22681 ttgctctcgc taacggtaaa cgcctggcgg tgcagaatcg cggtaaactc ttccataaag
    22741 ttgtcgtaat tcagttccag tttgtttacg cgctggttga gacggttata agccattacc
    22801 gccggaattg cggcgaacag accaattgcc gtcgcaatca acgcttctgc gataccgggc
    22861 gcaaccattt gcagtgttgc ttgttttacc gccccgaggg cgataaaggc gtgcatgatc
    22921 ccccagaccg taccaaacag accaatatac gggctgatgg agccaaccgt accgaggaac
    22981 ggaatgtgcg tttccagatt ttcaagttca cggttcatgg agatacgcat agcacgcgac
    23041 gccccttcca ctacggcttc cggcgcatgg ctattggcac gatgcaggcg cacaaactct
    23101 ttgaacccgc tgtaaaagat ttgttccgaa ccagtcagat tatcccgttt cccctgactc
    23161 tcttgataga ggcgagaaag ttcgattcca gaccagaatt tatcttcaaa cgcttcggct
    23221 tcacgcgccg ctgcgttaag aatacgggtt cgctggataa taatggccca ggatgcgatt
    23281 gaaaaaccaa tcaaaatcaa catgataagt ttaaccagaa ggctagcctt caggaacaaa
    23341 tcaaggatat tcatgtcagt cactgcttaa actccgcgac aatagacttg ggaagcgcac
    23401 gaggcttcat tttgagtggg tcaacgcaaa caaccagaac ctctgcttca ttcagcaaag
    23461 tattctcggc gttgacaata cgttgcgtga aaaccaaaga ggtgccacgc attgatgtta
    23521 tttcagtctg tatttcgagc atatcgtcga gccgcgcagg tgcgtaatat tccaccgtca
    23581 ttttacgtac cacaaaggca acgcgttcag ccatcagcgc ctgctgactg aagtgatgat
    23641 gacgcagcat ctctgtgcgt gctctttcat aaaaagcgac gtaactggcg tggtacacca
    23701 caccaccggc atcggtatct tcatagtaga cgcgaaccgg ccatcgaaac agcgttgtat
    23761 tcactttaca tcccggtaat gcaacaaaag ttagagcttt taaacgctgc tactatacgc
    23821 gcgcgattga ggtttgggaa tgagggcaag ttaagggcgc gtaaaaattt atgggcctag
    23881 agcggcccat aattttttaa agagaattct gatttaaaag aagaagaaaa tcagcccgac
    23941 aatgagaaca atatcggcaa gcaatgggca aaaaatccct tgccaaagaa ccttctgcgg
    24001 acgaaagccc acgccgtgaa tcacaccagc acagacggcc cacatcagca ataaaccatg
    24061 ccagatttcc agttcactgg tcttcgcggc aaaacgagat gggtcccaaa acatacatcc
    24121 tgctaacaga agcgccatca cgaaggaaag cgcccgcagg gggcgcttgt ccattaccgc
    24181 atacaaggtc gcgataatct tactcatcag atgtcttctt gaccggcttt gcctgattcg
    24241 acgtgttcaa gcgccagcgc ggtgattacc ccaaacgaac aggcaagaag cgttcccaga
    24301 atccatgcga aataccacat ttttagctcc ttacttagta cagagagtgg gtgttacgtt
    24361 caatatcttc tttggtgata cgaccgaaca ttttccagta acaccaggcg gtgtagagca
    24421 gaatgatcgg taccagaacc accgcaaccc aggtcatgac gttaagcgtc agctggctgg
    24481 aagttgcatc ccacattgtc agacttgcgt tcatcatggt gctggacggc atcacaaacg
    24541 ggaacattgc gataccggct gtcaggatga tgcaggccag cgtcagggag gagaacacaa
    24601 acgcccacgc ggctttatcc atacgtgcag tcaggatggt cagcagcagc agaaccacac
    24661 ccagtgccgg aatagcccac agaattggcg tgttgttgaa gttaaccagc catgcgccag
    24721 cttcacgaac cacttcttta ttcagtgggt tagaggctgc gtaatggtcc attgtcgatt
    24781 tcacgacata accatcgata ccgtacatca cccatacgcc agccagtgcg aaacagacca
    24841 gtgtcaccag cgcagccacc tgagccgttg cacgggtacg caggtgcagt tcgcccacgg
    24901 tacgcatttg cagataggtt gcgccctgag taatgatcat ccctacgctc accacgcctg
    24961 ccagcaggcc gaacgggtta agcaactgga agaagttacc ggtgtagtac agacgcagat
    25021 attcatcaac gttgaacggt acgccctgca acaggttacc gaacgctaca ccaattacca
    25081 gcggcggaac gaagctacca atgaagatgc cccagtccca catgttacgc cagcgggttt
    25141 cttcaatctt ggagcggtag tcaaaaccga ccggacggaa gaacaaagac gccagcacga
    25201 ggatcatcgc cacatagaag ccggagaacg cagcggcata gaccatcggc caggcagcaa
    25261 agagtgcgcc gcccgcggtg atcagccaaa cctggttacc gtcccagtgt ggtgcaatgg
    25321 agttaatcat aattcgacgc tcggtgtcgt tacgaccgag gaaacgggtg agcatgccca
    25381 cccccatgtc gaaaccgtca gtgactgcaa aaccaatcag cagaacgcca accagcagcc
    25441 accagataaa acgcaatact tcataatcga tcatttgacg actcctgtct tagcgtgccg
    25501 gctgagtagt cgtggaagac tgctcaaagt gatagcgacc ggttttcagg ctgcttgggc
    25561 cgaggcgtgc aaacttgaac attaagaaca attctgccac caggaacagg gtatacaggc
    25621 cgcaaatcag caccattgag aagatgagat cgcctgcggt cagtgacgaa ttcgccacag
    25681 ctgtcggcag cacttcaccg atagcccacg gttggcggcc atattcagcc acgaaccagc
    25741 ccgcttctac agcaatccac ggcagcggaa taccgtacag cgcggcgcgc agaagccatt
    25801 ttttctcgcc aatgcggttg cggatgacac tccagaaaga gagcgcgatg attgccagca
    25861 gcaggaagcc acacgccacc atgatacgga acgcaaagta cagcggcgct acacgcggga
    25921 tggagtcttt ggttgcctgt tgaatctgcg cttcagtcgc atcagccacg tttggcgtat
    25981 agcgtttcag cagcagaccg taaccgaggt ctttcttcat gctattgaac tggtcacgaa
    26041 ccgtctggtc ggtagaacca gaacgcagtt gttcgagcag agagtacgcc ttcatcccgt
    26101 tacgaatgcg ttcttcatgc tgcaccatca gctctttcag gccgataacc ggggtatcca
    26161 cggaacgcgt tgcaatgatg cccagtgcgt aagggatctg aatcgcaaat ttgttcgtct
    26221 cctcttcctg atcaggaatg ccgaacaaag taaaggcagc aggcgcaggt tgcgtttccc
    26281 actcggcttc aatagcagcc agtttggttt tctgcacgtc gcccatttcg tagccggatt
    26341 catcacccag aacaataaca gacagaacag cagccatacc gaagctggca gcgatagcaa
    26401 aggagcgttt agcgaaggcg aagtcacgac ctttcagcat ataccatgcg ctgataccga
    26461 ggatgaacat cgcgccagtc acataaccag acgctacagt gtgaacgaat ttcacctgag
    26521 caaccgggtt aagcaccagc tcggagaagc tcaccatctc catacgcata gtttcaaagt
    26581 tgaaatcgga cgcgattggg ttttgcatcc agccgttcgc aaccagaatc cacagtgcgg
    26641 acaggtttga accgagcgcc accagccagg tgacacacat atgctgaact ttacccagac
    26701 gatcccaacc gaagaagaac agacctacaa aggtggattc gaggaagaag gccatcagac
    26761 cttcgattgc cagcggcgca ccgaagatat cccctacata gtgggaatag taagaccagt
    26821 tagtcccgaa ctggaactcc atggtcagac cggtagccac acccagagcg aagttgatac
    26881 caaacaactt gccccagaac ttggtcatat ctttataaat ctgtttgccg gagaggacgt
    26941 agaccgtttc cataatggcc aggaggaacg ccataccgag cgtcagtggc acaaaaagga
    27001 agtggtacat cgcggtcaag gcaaactgta agcgcgacag ttcgactata tctaacatca
    27061 tgactccttg ctcatcgcat gaagactccg agagtgaacc ccgttagaaa gggtcacacg
    27121 catgccccaa tagaaatttg tttgaacact ttcatcatac catgtgaatg cacacaaaaa
    27181 cggcgtaaag aaaggttgca aacatgttaa taaaaactca aattgatccc acgtatatat
    27241 tacgccgcaa aatccttaca ataaacaggt ttttgttgaa gcaaatttgc atttttcgac
    27301 ggtgatcaat ttatagctaa attgccgcct ttcagccaat ttgatcgaga acaat
//
LOCUS       DAZSIO010000040        26963 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZSIO010000040 DAZSIO010000000
VERSION     DAZSIO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.040"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..176)
                     /locus_tag="ACHX5T_001549"
     CDS             complement(<1..176)
                     /locus_tag="ACHX5T_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR8466797.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="ACHX5T_001550"
     CDS             complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="ACHX5T_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR8466798.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1230..1526)
                     /gene="fis"
                     /locus_tag="ACHX5T_001551"
     CDS             complement(1230..1526)
                     /gene="fis"
                     /locus_tag="ACHX5T_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR8466799.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="ACHX5T_001552"
     CDS             complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="ACHX5T_001552"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR8466800.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="ACHX5T_001553"
     CDS             complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="ACHX5T_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR8466801.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3739..5190)
                     /gene="panF"
                     /locus_tag="ACHX5T_001554"
     CDS             complement(3739..5190)
                     /gene="panF"
                     /locus_tag="ACHX5T_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR8466802.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5180..5422)
                     /locus_tag="ACHX5T_001555"
     CDS             complement(5180..5422)
                     /locus_tag="ACHX5T_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR8466803.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5531..6880)
                     /gene="accC"
                     /locus_tag="ACHX5T_001556"
     CDS             complement(5531..6880)
                     /gene="accC"
                     /locus_tag="ACHX5T_001556"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR8466804.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6891..7361)
                     /gene="accB"
                     /locus_tag="ACHX5T_001557"
     CDS             complement(6891..7361)
                     /gene="accB"
                     /locus_tag="ACHX5T_001557"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR8466805.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAKSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8339..9313)
                     /locus_tag="ACHX5T_001558"
     CDS             complement(8339..9313)
                     /locus_tag="ACHX5T_001558"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR8466806.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9465..11405
                     /gene="csrD"
                     /locus_tag="ACHX5T_001559"
     CDS             9465..11405
                     /gene="csrD"
                     /locus_tag="ACHX5T_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR8466807.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11710..12753
                     /gene="mreB"
                     /locus_tag="ACHX5T_001560"
     CDS             11710..12753
                     /gene="mreB"
                     /locus_tag="ACHX5T_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR8466808.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12819..13922
                     /gene="mreC"
                     /locus_tag="ACHX5T_001561"
     CDS             12819..13922
                     /gene="mreC"
                     /locus_tag="ACHX5T_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR8466809.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13922..14410
                     /gene="mreD"
                     /locus_tag="ACHX5T_001562"
     CDS             13922..14410
                     /gene="mreD"
                     /locus_tag="ACHX5T_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR8466810.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14419..15012
                     /gene="yhdE"
                     /locus_tag="ACHX5T_001563"
     CDS             14419..15012
                     /gene="yhdE"
                     /locus_tag="ACHX5T_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR8466811.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15002..16471
                     /gene="rng"
                     /locus_tag="ACHX5T_001564"
     CDS             15002..16471
                     /gene="rng"
                     /locus_tag="ACHX5T_001564"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR8466812.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16539..20339
                     /gene="yhdP"
                     /locus_tag="ACHX5T_001565"
     CDS             16539..20339
                     /gene="yhdP"
                     /locus_tag="ACHX5T_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR8466813.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20495..21940
                     /gene="tldD"
                     /locus_tag="ACHX5T_001566"
     CDS             20495..21940
                     /gene="tldD"
                     /locus_tag="ACHX5T_001566"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR8466814.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="ACHX5T_001567"
     CDS             complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="ACHX5T_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFR8466815.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23180..23383
                     /gene="aaeX"
                     /locus_tag="ACHX5T_001568"
     CDS             23180..23383
                     /gene="aaeX"
                     /locus_tag="ACHX5T_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR8466816.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23391..24323
                     /gene="aaeA"
                     /locus_tag="ACHX5T_001569"
     CDS             23391..24323
                     /gene="aaeA"
                     /locus_tag="ACHX5T_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFR8466817.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24329..26296
                     /gene="aaeB"
                     /locus_tag="ACHX5T_001570"
     CDS             24329..26296
                     /gene="aaeB"
                     /locus_tag="ACHX5T_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR8466818.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTHFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26388..26660
                     /locus_tag="ACHX5T_001571"
     CDS             26388..26660
                     /locus_tag="ACHX5T_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR8466819.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26716..>26963)
                     /gene="yhcN"
                     /locus_tag="ACHX5T_001572"
     CDS             complement(26716..>26963)
                     /gene="yhcN"
                     /locus_tag="ACHX5T_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR8466820.1"
                     /translation="VAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSGVASSPM
                     DIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6425 a         6771 c         7215 g         6552 t
ORIGIN      
        1 attctgtcct gaaaattcag ttcgtcacag ttaaccagtt ttagtggatt aactgagttc
       61 atattacggc atttgtctgt ttctgttgtc agcagttcaa tccactgcat cagtaaagta
      121 tcaaacagaa aaacgaagac agcgaaaacg acgagccacc aatacttgcg aatcattacc
      181 tgagcctgtg catgtttaac caagtctggc aggaaatata cacgaaatga ataatctgaa
      241 aactaggctt acacttcctt tactttgcaa tgagatcggg gtcaacttcg cttaaccggc
      301 ttcgttttga caggttgccg gtctttcttt ttttcacttt cgccagttct tccgcggagt
      361 aatgcgacgc gacatccagc cgtcgaagcc ccattttgat gtactcgctg ttgatctcaa
      421 taccaatgaa ttttcgtccg ctggcgatgg ctacggcacc ggtagtaaag ctaccggcaa
      481 acgggtcgag aacgatatcg cctgggttgg aagaggcgag aataatgcgt ttcagtaagg
      541 ctttcggttt ttgcgtcggg tggttctcat attcatccat caaataacgc acgcgcggaa
      601 aatcccagac gttacccggt actttttgat gattgtatgg ctgtggagga tttttgcgat
      661 aatcgatcaa cgcgcgctgc gatccggttt tggcttctac cagaatagca tcaccgttga
      721 atgtgtagtt ctttgcgtct ttcaccatca tcaggatggg ttcgtacatg gagccgtagt
      781 gttttttcgc ctgtactcca gaactgtcat atgaccagac gatgcgactt ttgatggtaa
      841 aaagcttgcg gcactggaga tcgataaagg gcatgttttc cgtactgttc ataatgtaca
      901 tgctgccctg ctttttcaga acgcggtggc actctgcaat cacttcaaac agccagtcga
      961 taaacagatc ttctttccag gcttcgatca gaccatcaaa atttttaccg atgttatacg
     1021 gtgggtcggc aaagagcaga tcgacgcttt cagtaggtag ctttttaagt tcggcaaagg
     1081 catcaccgtg aataatggtc ttagcttcat taccaaaccg ggtcggttca catcctgttc
     1141 tcatggtcac tccctttgtg acacctataa aaaaggcgct tccccatgcc gagtagcgcc
     1201 tttttaatca agcatttagc taacctgaat tagttcatgc cgtatttttt caatttttta
     1261 cgcagcgtac cacggttgat gcccatcatc agcgcagcac gggtctggtt accacgggtg
     1321 tattgcatca ccatgtccaa caggggctgt tctacttcag ccagtaccag ctcatagagg
     1381 tcattcacat cctgaccatt cagttgagca aaatagttct tcagtgcctg tttaaccgag
     1441 tcacgcaggg gtttttgggt tacctgatcc tgagagttaa cggtagaaac ggtcagtacg
     1501 tcagaattta cgcgttgttc gaacatagtt ctgtcagctc tttatttctg tttacgcaaa
     1561 attttcgaag tatgcctcca acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa
     1621 tgtgcgccga aactggtcat ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg
     1681 tgcaattcgg tacccttttg ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca
     1741 aagcaagcgc ttaacctctg ccaaaggcag tgggggcagc aactccccag tgtccagata
     1801 atgctggatt tcccgaaaga tccagggtct tccctgagct gcgcggccta tcatcagggc
     1861 atccgcccct gtatagtcga gcacagctct ggctttaagc gggtcagtaa tgtcgccatt
     1921 cgcgataacc ggaatggaaa ctttctgctt aactgcccga atactgtcgt actcagcttc
     1981 tccattgaac aaacaggcgc gtgtacggcc atgaatggtc agagcctgaa tgccacagtc
     2041 ttcagccagt tgggcaatct cttcgcagtt acggtgttcc ggtgcccagc cggtgcgaat
     2101 cttcagggta acaggaacgt ccactgcatt gacgacctcg gtaaggatcg atttaacgac
     2161 atccgggtac tgcaagaggg ctgaacctgc gagcttgcga ttcacttttt tagccgggca
     2221 acccatattg atatcaataa tctgggcacc gctttccacg ttaatacgtg ctgcatctgc
     2281 catttctttc ggagcgctac cagcaatttg cacggtgcga ataccgggtt catcaatgtg
     2341 caccatccgt aaacgagatt tgtcgctttc ccaaacctgt gggttagaag acatcatctc
     2401 ggatactgtc aatccggctc ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat
     2461 gccagccatg ggcgctgcga tcaggcgatt tctgagctga tattgtccga tgcgcatgag
     2521 ttaagaaatg accatactgt gactgcaagg cggcgtatat tacgcatttt ttgcacgaga
     2581 tgaaaggcca aactttgacc aatcctctga gatggatcaa agaattgcat ttaaaatgag
     2641 cgtggtgcga taattactca taaaaatcat catattagaa aatagtgact aaaatttaca
     2701 ctcaaagaaa tttgagtaag ttctcaattt ttctttatga atgaaaattt tggcacgcaa
     2761 atttgcgtaa ataatcggca aatttacgtg ccttttgtga gcttgctcgc acttcgcccc
     2821 gcgtcaccct acggcgatgc gaaggtcaat tcttacgacc ggtaatacgg caccactctt
     2881 ctttttccac gaccgggtcc agtgcgaagc tatcggcata agcttcacaa acgctctctg
     2941 cctggcttgc cagaataccg gaaaggccca gcaaaccgcc tgaaactggc aggacgctga
     3001 ttaacggtgc cagttcacgt aatgggcctg caaggatgtt agcgaccacc acgtcggctt
     3061 tcatttcttc tggctgatct ttcggtaagt agagttccag acggtcagaa acgccattac
     3121 gttcggcgtt atcgcggctg gcctgaatcg cctgcggatc gatatcaata ccaatggctt
     3181 ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag ccaaagtcga
     3241 tgactgtttt accggttaaa tcgaggctgt cgagccattg caggcacaga gaggtggttg
     3301 gatgggtacc cgtaccaaac gccagccctg gatctaacat cacgttgacg gcgttttcgt
     3361 ccggcacatc acgccagcta gggcagatcc acagtcgttc accaaagcgc atcgggtgga
     3421 aattatccat ccattcgcgc tcccagtctt tatcttctag ttgttcgatt ttatgcgcga
     3481 agcctgcgcc gagcagcgga tggttttcca gaatcgccac cacgtcgttc atatcggttt
     3541 cagcgtcgaa cagaccaatc acatcggtgt cgccccacag gcgcgtttcg cccggcagcg
     3601 gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat agaaacggca cccgcttcca
     3661 tcagcgcatc gctaagatct tccgcgttcg cgccggtggt gttcagtttc agttggatcc
     3721 aaggcatggc aaaactcttt atttatcagt agtcaaaacg gtagcttgcg ggacggatgt
     3781 accgaaacgg tttccgacca ggaaagccag caaacttagt agtaacgagg gcacgatagg
     3841 gtggaagccc aggtactgaa tattcagcgt cgcgagtacg gcatacagca cgccgccaac
     3901 gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag
     3961 cggccacagg aaaacggctt ccagcccacc gaaggccagc aaattcagcc agatgatcat
     4021 ttctggcggc ttccaggcgg caagcagcag caacgcgccg agaactaacg taattaccgc
     4081 cgacatccgc ttcagacgcg tctcgttttg catttgatcc ggacggatat tcagatagag
     4141 atctttaatg atcgtagcgg aactttgcag cagttgggcg ttaattgtcg acatgatcgc
     4201 agccataggt gcagccagaa agatcccggc agcaaaaggt ggcagcactt ttaccattaa
     4261 cgtcgggatc accagatctg gtacggtgag atcggggatc actgcacgac ctaacgctcc
     4321 ggccaggtgc ataccgaaca tcaggattgc gactataatc gtaccgatga tgatcccccg
     4381 gtgtactgct ttgctgtctt tataagagat acagcgcacc gcagtatgcg gcaggccaat
     4441 cacgccaaag cacaccagta cccagaacga cgtcataaag gcaggcgaca gaatatcgtc
     4501 agcgccttgt ggcgtaacca gttgcggatc gatggtttgc aaggtctgta ctgcgttgct
     4561 taagccgcca gcggcatgta ccacgccaat aagcagcaca acggtgccaa tcagcatcac
     4621 aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg cggtatataa
     4681 cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa ggaatacccg ccgcggtttc
     4741 cagcaggcgc gcaccgccga taaactgcac ggtcatcgca ccaacgaacg caaccagcaa
     4801 actcaaactc gccagccaca ccagaagacg actctggtag cgggcaaaca gcatatcgtt
     4861 cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag
     4921 tgaaagccag acagcaggaa gctgaatcat cgccagcaat acccagccca gcccgtattt
     4981 ataagcagct cctggcccgc cgataaacga actggcactg atataggtcg cggtgagagt
     5041 catcgccagc acaataccgc ccatagagcg gctgccgagg aaatactcat taaggaaggt
     5101 gccggtgctc cgtttacgca tcgcataaac cgagataccg aacaccacca ccagataggc
     5161 gaccagcggt agaattactt caagctgcat cgtcatcctc cagtgggata tcgcgataga
     5221 taaatttcac catcgcccag cacagtccaa taaacagtag cggcgtcagg atgcaggcca
     5281 tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag taagcgtcta
     5341 ctaaccaaac tgccagatac aaaagggtca gccccagcgc ccagcgggcc tctttatggg
     5401 cctgaacaaa acgagtgtcc attttttgtc cctgatgggt gaagaaagcg aggattgtac
     5461 cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc cttttgacgc
     5521 ttttgcagtc ttatttttcc tgaagaccga gttttttctc cagatagtgg atgttagtgc
     5581 caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg ttggttttga
     5641 taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc acgtcacggt
     5701 tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt acggtgtagc
     5761 ccgcgtagat atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga
     5821 ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc gttgatacga cattccaccg
     5881 catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg gcagcgatac
     5941 gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg tgttctacct
     6001 gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg aactcgaaag
     6061 tacctgcacc gcgatagccg atatcaacac acgctttagc gcaacgttcg ccgatgtagc
     6121 gacgcagttc cggggtaatg cccggtgctg gtgcttcttc gaccactttc tggtggcggc
     6181 gctgcatgga gcagtcacgt tccgccagat agatagcgtt gccctgaccg tcagccagta
     6241 cctgaatctc gacgtggcga ggattctcca ggtatttctc catgtaaacc atatcgttgc
     6301 tgaaagcagc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc
     6361 cgcgcactac gcgcataccg cgaccgccgc cgccgccgga ggctttgata atcaccggat
     6421 aaccaatgcg tttagcaatg gcgcggtttt tatccatatc gtcgcccagc gggccgtcag
     6481 aaccaggtac gcaaggaacg cccgcttttt tcatcgccgc gattgcggat actttgtcgc
     6541 ccatcaggcg aatggtttct gctttcgggc cgatgaagat aaagccggag cgttcaacct
     6601 gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt gctactgcgc
     6661 cggtgatttc agcggcgctg atgattgccg ggatgttcag ataacttttt actgacggag
     6721 cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg cgatccgtgc
     6781 tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga atacgcaatg
     6841 caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg ttactcgatg
     6901 acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat tgctttcacg
     6961 gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac gatgcacagg
     7021 gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcgtc cgggcttggg
     7081 gtgcggtaga aagtaccaac catcggggaa cgtacgatgt gaccactgat ttccgctgct
     7141 gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattt agctggctgc
     7201 tgcatcattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc aggagctgca
     7261 cggctaatgc gtactgactc ttcgccttca gaaatttcca gttcggagat gcctgattct
     7321 tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt ccgtactctt
     7381 tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc
     7441 tgtatgcata gcgataaatt ccagcaggcc gggacgccgg tctattttgc cccagagtgt
     7501 caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg caaaaaacaa
     7561 aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc gtcagggagg
     7621 acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg gtcttatcag
     7681 ggaatataat caacagctaa catgtaaata accttcaaca ccgtgtaatt tgcaacaagc
     7741 cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt aaggtgaaaa
     7801 gctgaaaagc cagccctgcg aagatgaggg gctgcaatga gataatcttc acagaccaca
     7861 aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc ggttgctggg
     7921 gattacccaa ttttcactgc atcgtctgga tagatgtgaa tgctaaagca cgcaaaatca
     7981 accaactgac aattctcagc attaaatagg ttcgagtggt taatttgtag tcaaataaaa
     8041 tcagattatt caccccatgg taccagaaat cagtaacagg ttaagcgtag tgtcgcccag
     8101 gcaaagcacc ataaacctaa caggcgacgg aggcgtatca ataatggctg tttagaggag
     8161 tatcaatgcg catcaatttg tggtcagtcg aaatcatgac tttccagttt ggatgatgtt
     8221 gtacaacttc ctgaccaagg tgttcgacga aagctgtccg gctaaccgct tttcccgtta
     8281 accgattgaa taatgatgtc aaaatgagtt ccctctctta ttattccttg ctaaatggtt
     8341 agttaacctt caccagcgtg cgaccctgga tctggttatt aatgatggcc tcggcaaagt
     8401 tcggtgcctc tgacagagat atctcttttg ccgcctgggt atagaatgat tccggtaaat
     8461 cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca
     8521 ccccttgcaa acggacatta cgcagaataa atggcatgac cgtggttggc agagtaaaac
     8581 cacccgccag accacaggcc gccacgcagc cgccgtaatt catttgcgcc agcacttttg
     8641 ccagcacttt gtcgccaaca gtgtcaattg ccccagccca gacttgtttt tccagaggac
     8701 gggattcggc aaactcatca cgagggagaa taccgctggc acctaaactt ttcagatatt
     8761 catgggtact ttcgcgaccg gaaacggcaa cgacctgata acccaactta tgcagcagcg
     8821 ccacggcggt actgccgacg ccaccactgg cacccgtcac gacaatctca ccgtcctgcg
     8881 ggcgaacacc ggcatcttcc agcgccatca cacacagcat ggcggtaaaa ccggcagtac
     8941 cgataatcat tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt
     9001 tcactcgcgc ctgctccgcc agcccacccc agtggttttc accaacgccc cagccagtga
     9061 gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa
     9121 aatcgatccc aggaatcatc ggaaaattac ggatgatttt tcccttaccg gtaatcgcca
     9181 gcgcatcttt atagttcagg ctcgaccagt gaacatcgac cgtgacatcg ccctccggca
     9241 ggcgacgttc gtccagagtc tgtactgatg cgagagtttt gccgtcctgc tgttctaaaa
     9301 gtaacgcctg cataagtggt cctcatgtgc atgatggatt ggaaaataat ttctgaagac
     9361 tatactcgct aatggaaaag caaagccgat gaagcgcaat aaattgcgag ataaatctga
     9421 tttgctagta tgcccgcttc ctcactatcg gagttaacac aaggatgaga ttaacgacga
     9481 aattttcggc ctttgttacg ctgctcaccg ggttaacaat ttttgtgact ttgctgggct
     9541 gttcgctaag tttctacaac gccattcagt ataagtttag tcatcgcgtt caggcggtgg
     9601 cgacggcgat tgatacccac cttgtgtcga atgacttcag cgcattaagg ccacaaatta
     9661 ccgaattaat ggtgtcagca gatatcgttc gtgtagacct gctgcatggt gataaacagg
     9721 tttataccct ggccagaaat ggtagttatc gtccggttgg caccagcgat ctgtttcgtg
     9781 aactgagcgt tccgttgata aagcatccgg ggatgtcgtt gcgtctggtt tatcaggatc
     9841 cgatgggcaa ctatttccat tcgttgatga ccaccgcgcc gctcacgggg gcgattggct
     9901 ttatcattct tatgctcttt ctggcggtac gctggttaca acggcaactt gccgggcaag
     9961 aattgctgga aacccgggct actcgtatct taaacggtga gcgtggctct aatgtgttgg
    10021 gaaccatcta tgaatggccg cccagaacca gcagtgcgct ggatacgctg cttcgtgaaa
    10081 ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct gatccgctct tatgccgcac
    10141 aggacgtgaa aaccggcctc aataaccgac tctttttcga taatcagtta gcaacgttac
    10201 tggaagatca ggagaaagta ggtacccacg ggatcgtgat gatgattcgt ctgccggatt
    10261 tcaatatgtt gagcgatacc tgggggcaca gccaggttga agaacagttc ttcactctga
    10321 cgaatctgct gtcgacattt atgatgcgct accctggcgc actgctggcg cgttaccacc
    10381 gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag agcatagcca
    10441 gtcagttaat caaagccgtc gataccttgc cgaacaataa aatgctcgat cgcgacgata
    10501 tgatccacat tggtatctgt gcctggcgta gtggtcagga taccgagcag gtaatggaac
    10561 atgcagagtc tgccacgcgt aatgcgggat tgcagggtgg caatagctgg gctatttatg
    10621 atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa
    10681 tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg
    10741 ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa tgaagaggtt agctcggcgg
    10801 agtatatgcc gatggtcttg cagtttggct tatcggaaga gtatgaccgt ctgcaaatca
    10861 gccgtcttat tccactattg cgttactggc cagaggaaaa tctggcgatt caggttaccg
    10921 ttgagtcgct gattcgtccg cgttttcagc gttggctgcg cgatacgtta atgcaatgtg
    10981 aaaaatcaca acgaaaacgc ataattattg aacttgcaga ggccgatgta ggtcaacata
    11041 tcagtcgttt acaacctgtt attcgtttag tgaatgcttt aggggtacgg gtagccgtca
    11101 accaggctgg tttgacgctg gtaagcacca gttggatcaa agaacttaat gttgagttac
    11161 tcaagctcca tccggggctg gtcaggaaca ttgagaagcg aacggagaac cagctgctgg
    11221 ttcaaagcct ggtggaagcc tgctccggga ccagcaccca cgtttacgcc accggcgtgc
    11281 gttcgcgaag cgagtggcag accctgattc agcgcggtgt tacaggcggg caaggggatt
    11341 ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa atattcacaa agatactcgg
    11401 tttaacctgc cgtttaatcc gttttcacgt agaataacgc gcgctgcgtc tcatgggagt
    11461 gtgcttgtct gctcgccaga ttgttgcagc acatatgcag atgaatgacc ttacgcggtt
    11521 gcaaacaggc gaggaatgct gctgatgcat taagcctttc tggactcagg cagagatttg
    11581 taacaaagga aacgaactgc actaattttc accgtagcag atgatttttg cgccttgtcg
    11641 ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc cgccccagct ttcaggatta
    11701 tcccttagta tgttgaaaaa atttcgtggc atgttttcca atgacttgtc cattgacctg
    11761 ggtactgcga ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc
    11821 gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat
    11881 gacgcgaagc agatgctggg ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa
    11941 gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc tccagcactt catcaaacaa
    12001 gtgcacagca acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc
    12061 gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa
    12121 gtcttcctga ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa
    12181 gcgaccggtt ctatggtggt tgatatcggt ggtggtacca ctgaagttgc tgttatctcc
    12241 ttgaacggcg tggtttactc ctcttccgtg cgcattggtg gtgatcgttt cgacgaagct
    12301 atcatcaact atgtgcgtcg taattacggt tctctgatcg gtgaagccac cgcagaacgt
    12361 atcaagcacg aaatcggttc ggcttacccg ggcgatgaag tccgtgaaat cgaagttcgt
    12421 ggccgtaacc tggcagaagg tgttccacgc ggttttaccc tgaactccaa cgaaatcctc
    12481 gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg taatggttgc actggaacag
    12541 tgcccgccgg aactggcttc cgacatctcc gagcgcggca tggtgctcac cggtggtggc
    12601 gcactgctgc gtaaccttga ccgtttgtta atggaagaaa ccggcattcc agtcgttgtt
    12661 gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac
    12721 atgcacggcg gcgacctgtt cagcgaagag taatcggatg caggcagggg aagcgtctgt
    12781 ttaccctgcc tggtctgata cgagaatacg cataacttat gaagccaatt tttagccgtg
    12841 gcccgtcgct acagattcgc cttattctgg cggtgctggt ggcgctcggc attattattg
    12901 ccgacagccg cctggggacg ttcagtcaaa tccgtactta tatggatacc gccgtcagtc
    12961 ctttctactt tgtttccaat gctcctcgtg aattgctgga tggcatatcg cagacgctgg
    13021 cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa
    13081 acagtgaact gctgatgctt ggacaataca aacaggagaa cgcgcgtctg cgcgagctgc
    13141 tgggttcccc gctgcgtcag gatgagcaga aaatggtgac tcaggttatc tccacggtta
    13201 acgatcctta tagcgatcaa gttgttatcg ataaaggtag cgttaatggc gtttatgaag
    13261 gccagccggt catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga
    13321 ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct gccaatccag gtgctgcgca
    13381 acgatatccg cgtaattgca gccggtaacg gttgtacgga tgatttgcag cttgagcatc
    13441 tgccagcgaa tacggatatt cgtgttggtg atgtgctggt gacctccggt ctgggcggtc
    13501 gtttcccgga aggctatccg gtcgcggttg tctcttccgt aaaactcgat acccagcgcg
    13561 cttatactgt gatccaggcg cgtccgactg caggactgca acgtttgcgt tatctgctgc
    13621 tgctgtgggg cgcggatcgt aacggcgcta acccgatgac gccggaagag gtgcatcgtg
    13681 ttgctaatga acgtctgatg cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg
    13741 ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc gactccgcag caaccggcga
    13801 caggaaatgc agctactgcg cctgttgcgc cgacacagcc cgctgctaat cgctctccac
    13861 aaagggctac gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg ggagggcaat
    13921 agtggcgagc tatcgtagcc agggacgctg ggtaatctgg ctctctttcc tcattgcgct
    13981 gttgctgcaa atcatgccct ggccggataa cctgattgtt ttccggccaa actgggtgtt
    14041 acttatcttg ttgtattgga tcctggcctt gccccatcgc gtaaatgtgg gcacaggttt
    14101 tgtgatgggt gccatactgg atctgatcag cggctcgatg cttggcgtac gcgtattggc
    14161 gatgagcatc attgcttacc tggtggcgct gaaataccag cttttccgca acctcgcatt
    14221 atggcagcag gcgctggtcg tcatgttgct ttcgctggtg gtggatatta ttgttttctg
    14281 ggcagagttt ttagtgatta acgtctcttt cagaccggaa gtgttctgga gtagtgtagt
    14341 caatggggtg ctctggccgt ggattttctt gctgatgcgt aaagtccgtc agcagtttgc
    14401 agtgcaataa aggtttctat gacttctctg tatttagctt ccggttctcc gcgtcgtcag
    14461 gagttacttg cgcaacttgg cgtgaccttt gaacgtattg ttacgggcat tgaggagcag
    14521 cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc tggcacgcga gaaagcacag
    14581 gcgggtgtcg cgcaaacggc acaggatctc ccggtgctgg gtgcggatat tatcgttatc
    14641 ctgaacggag aagtgctcga gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc
    14701 aaattatcgg gtcagaccca tcaggtgatg acggcagtgg cgttggccga cagccagcac
    14761 attctcgatt gcctggtggt caccgatgtg actttcagaa cgttaacaga cgaagacatc
    14821 gcgggctatg tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg tattcagggg
    14881 ctgggtggct gttttgtcag gaagataaat ggcagctatc acgccgtagt cggcttaccg
    14941 ctggttgaaa cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa aagggataaa
    15001 catgacggct gaattgttag taaacgtaac gccttcggaa acgcgagtgg cgtatattga
    15061 tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa
    15121 tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt ttgtagatat
    15181 tgggctggat aaagccgcgt ttcttcatgc atccgacatc atgccgcata ccgaatgtgt
    15241 ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc tcggaactgg ttcgtcaggg
    15301 gcaagatctg atggtgcagg tggtgaaaga tccgcttggc actaaaggtg cgcgcctgac
    15361 caccgatatc acgctgcctt ctcgctatct ggtatttatg ccaggggctt ctcacgttgg
    15421 ggtttcccaa cgaattgaaa gcgaatcaga acgtgaacgt ctgaaaaaag tggtcgcaga
    15481 gtattgcgac gagcagggcg ggtttatcat ccgcaccgca gcggaagggg ttggcgaggc
    15541 tgaactggcc tccgatgccg cttatctgaa acgcgtctgg accaaagtta tggagcgtaa
    15601 aaagcgcccg cagacccgtt atcagctgta cggcgaactg gcgctggcgc agcgtgttct
    15661 gcgtgatttt gccgacgccg aactggaccg cattcgcgtt gactcacgcc tgacttacga
    15721 agcattgctg gagtttacct cggagtacat tcccgagatg accagcaagc tggagcatta
    15781 caccggacgc cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgtt
    15841 ggaacgcaaa gtagaactga aatccggtgg ttatctgatt atcgaccaga ccgaagcgat
    15901 gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat cgcaatctgg acgacactat
    15961 tttcaatacc aatattgaag cgacgcaggc tatcgctcgc cagttacggt tgcgtaatct
    16021 gggcgggatt atcattattg atttcatcga tatgaataat gaagatcacc gccgccgcgt
    16081 gctgcattcg ctggagcagg cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt
    16141 ttcggcgctg gggctggtgg agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt
    16201 actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt
    16261 atgctatgaa atcatgcgcg agattgttcg tgtccaccat gcttacgact ccgaccgttt
    16321 cctggtctat gcttctccgg cagtagccga agccttgaaa ggcgaagagt cacactcgct
    16381 ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta caaattgaac cgctctataa
    16441 ccaggagcag tttgacgtcg taatgatgta aacagatgct gggccgccat ccggcaaagg
    16501 gtttttgagt cacattttta gcagacaagg agtgacgggt gaggcgattg ccggggattt
    16561 tactgcttac tggagccgcg ctcgttgtga tcgctgccct gctggttagc ggcctgcgta
    16621 ttgctttacc gcatcttaac gcctggcgtc cggaaatcct caacaaaata gaatccgcga
    16681 ctggcatgcc ggtagaagcc agtcagctct cagccagctg gcagaatttt ggcccgacgc
    16741 ttgaagcaca cgacatccgt gcagaactaa aagatggcgg cgaattttcg gttaaacgcg
    16801 ttactctggc gctggatgtc tggcagagcc tgttacatat gcgctggcag tttcgcgacc
    16861 tcactttctg gcagctgcgc tttcgcacca acactcctat caccagcggt ggtagcgatg
    16921 acagtctgga agccagtcac atcagcgatc tgtttcttcg tcaatttgac catttcgatc
    16981 tccgcgacag tgaagtcagt tttctgacgc catccggtca gcgcgccgag ctggcgatcc
    17041 cacaactcac ctggctgaac gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct
    17101 ccagccttac cggacagcac ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg
    17161 ggttgttaag caatggtcgc gtctggctac aggcggatga catcgacctg aagccgtggc
    17221 tcggtaaatg gatgcaggac aatattgctc tggaaacggc acagttctcc cttgaaggct
    17281 ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg
    17341 ccagctggtt gggcgagaag caaacgcata cgctgtcggt ggataatctg accgcgcata
    17401 ttacgcgtga aaatccgggc tggcagttct ctattcccga tacacggatc acgatggacg
    17461 gcaaaccctg gccgagcgga gcattgacgc tggcctggat accggaacag gacgttggcg
    17521 gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc tagtaatctg gagctggcag
    17581 gcctggaggg catacgcccg ctggccgcga aactttcacc tgcactgggt gatgtttggc
    17641 gctccacaca accgagcggc aagattaaca ctctggcgct ggatatcccg cttcaggcgg
    17701 cagacaagac ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa tggaaattat
    17761 taccgggtgc ggaacacttc tccgggacgc tttccggcag cgttgaaaat ggtttgctta
    17821 ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt attccgtgcg ccactggaaa
    17881 tcgccgacgg ccaggcaact ataagctggc tgaacaatga caaaggtttc cagctggatg
    17941 ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt cgttacctgc
    18001 aacctgctaa cgatgaaccc tggctgggta ttctggctgg catcagtacc gatgatggtt
    18061 cacaagcctg gcgctatttc ccggaaaact tgatgggtaa agacctggtt gattatttaa
    18121 gtggtgcgat tcagggcggt gaagcggata acgcgacgct ggtttatggt ggcaatccgc
    18181 aactcttccc ctataaacac aacgaaggtc agtttgaagt gctggtgccg ctgcgcaacg
    18241 cgaagtttgc cttccagccg gactggcctg cattaactaa ccttgatatt gaactggact
    18301 ttattaacga cggtttatgg atgaaaaccg atggcgttaa tctgggcggc gtgcgcgcga
    18361 gtaatctcac cgcagtgatc cctgactact caaaagaaaa actgctgatt gacgctgaca
    18421 ttaaaggtcc gggtaaagcc gttggccctt actttgatga gacaccgctg aaagattctc
    18481 tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc ttacatcttg
    18541 atatcccgct gaacggcgaa ctggtaaccg cgaaaggtga agtgacgctg cgtaataaca
    18601 gtctgtttat caaaccactc gacagcatcc tgaaaaattt gagcggtaaa ttcagcttta
    18661 tcaatggcga tctgcaaagt gaaccactga cagcaagctg gtttaatcag ccgttgaacg
    18721 tggatttttc caccaaagaa ggggcaaaag cctaccaggt tgcggtgaat ctcaacggta
    18781 actggcaacc ggcgaaaacc ggcgttctgc ctgaagcggt gaacgaagca ttgagtggca
    18841 gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta tcatgctggc gcgacctata
    18901 acgtagagct gaacggcgat ctgaataatg tgagtagtca cttaccttca ccgttagcca
    18961 aacctgcggg tgaaccacta gcggtaaacg ttaaggttga tggcaatctc aacagctttg
    19021 aattaaccgg acaggctggt gcggataacc atttcaatag ccgctggttg cttggtcaaa
    19081 agctgacgct cgatcgtgct atttgggcgg cagacagtaa aacgctcccg ccgttgccgg
    19141 aacaaagtgg cgttgaactt aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt
    19201 tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag tttcccacaa cacataacgt
    19261 tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa taacctgagt attgtttcgc
    19321 aaccgacggc aaatggcacc ctggttgagg cgcaagggcg tgaaatcaac gccacgctag
    19381 cgatgcgtaa taacgcgccg tggctggcga atatcaaata tctttattac aacccgagcg
    19441 tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt cccgacaacg gagcgcatta
    19501 acttccgtgg ctggccggac gcacaaatac gatgcacaga gtgctggttc tgggggcaaa
    19561 aattcggtcg cattgacagt gatatcacca tttctggcga tacgttaacg ctgaccaatg
    19621 gactgattga tactggtttc tcgcggctta ctgccgatgg tgaatgggtt aataatccgg
    19681 ggaatgaacg tacctcgctg aaaggaaaac tgcgtgggca gaaaattgat gccgccgcag
    19741 aattttttgg tgtcacgacg cccatacgcc agtcgtcatt taatgtggat tacgatttac
    19801 actggcgcaa agcaccctgg cagccagatg aagcgacgtt gaatggcatc attcatactc
    19861 aactgggtaa aggcgaaatt accgaaatca ataccggaca tgccgggcaa ttgctgcgct
    19921 tattgagcgt agatgccctg atgcgtaagc tgcgttttga tttcagagac acttttggcg
    19981 aagggttcta ttttgactcc attcgcagca ccgcgtggat taaagacggc gttatgcaca
    20041 ccgacgacac gctggtggat ggcctggagg cggatatcgc catgaaaggg tcggtaaatc
    20101 tggtacgccg cgacctgaat atggaagcgg ttgtcgcacc agagatttct gcgacggtgg
    20161 gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc ggcagtgttt gccgccagta
    20221 aagtgctggg gccgctgtgg agcaaagtct ccattttgcg ctatcacatt tcgggtccgc
    20281 tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat
    20341 ttgacgaggg cgcgtaattg ccccaatctc atatgataat cgttgccaaa ggccaacgag
    20401 ccggaacata actgtaggcc ggatagggcg ttcacgccgc atccggcagc cgtaaaaaat
    20461 cctctactgc agtaactaac gagtagcaaa aacgatgagt ctgaacctgg taagtgaaca
    20521 attgctagcg gcgaacggcc tgaaacatca ggacttgttc gcgatcctcg gtcaactggc
    20581 cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg agctatcacg aatcctgggt
    20641 tttagaagac cgcattatta aagatggttc ttacaacatc gatcagggcg ttggcgtgcg
    20701 tgcaatcagc ggtgaaaaaa ccggatttgc ttacgctgac caaatcagcc tgctggcgct
    20761 ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat agtggtgatg gcaaagtaca
    20821 gacgctgggc gcggtagagc atagcccgtt gtatacctcg gtagatccgc tgcaaagcat
    20881 gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat aaggttgctc gcgaagcgga
    20941 caagcgcgta caggaagtga ctgccagcct cagtggtgtc tatgaattaa ttctggttgc
    21001 ggccaccgac ggcacgctag cggcggatgt ccgtccgctg gtgcgtcttt ccgtgagcgt
    21061 tctggtcgaa gaagatggca aacgcgaacg cggtgccagt ggcggcggcg gtcgttttgg
    21121 ttatgagttc ttccttgccg atcttgacgg cgaagtccgt gcggatgcat gggcaaaaga
    21181 agcagtgcgt atggcgctgg tcaatctttc tgccgttgct gcaccagcgg gcaccatgcc
    21241 ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag gtcacggtct
    21301 ggaaggcgac ttcaaccgcc gtggcacttc agtatttagt ggacaggtcg gggagctggt
    21361 ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg gtcgatcgcc gtggttcggt
    21421 ggcgattgat gatgaaggta cgccaggcca gtacaacgtg ctgattgaga acggcattct
    21481 gaaaggctac atgcaggata aactcaacgc gcgtttgatg gggatgacgc cgactggcaa
    21541 cggtcgccgt gaatcctacg cccatctgcc catgccgcgt atgaccaaca cctatatgct
    21601 gccgggtaaa tcgaccccgc aagaaattat tgaatccgtt gagtacggta tctatgcacc
    21661 gaactttggt ggcggtcagg tggatatcac ctccggcaaa ttcgttttct ccacttcaga
    21721 agcatatctg attgaaaacg gtaaagtaac gaagccggtg aaaggcgcaa cgttgattgg
    21781 ttccggtatc gaaaccatgc agcagatttc gatggttggc aacgacctga aactggataa
    21841 cggcgtgggt gtctgcggta aagaagggca aagtttgccg gttggcgtgg gccagccaac
    21901 gttgaaagtc gataacctga ctgttggcgg tactgcgtaa taatttcatt attttcagaa
    21961 ggataattaa tctttctacg tgcacgaacg gtcccctcgc tcctctgggg ttagggtgag
    22021 gggaacccgt tggcacaggt ttgtagaacg taacagtaca atatgaatta cttctctttc
    22081 ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa caaaataatc cgtcagcgag
    22141 ttgatcacga cctgtacctt cagcggcagc ttatcttttt cggtatataa cgcataaacc
    22201 gggcgtggat ctgactgata acgcggcagc aggatctcca gctccccacg attgatctcg
    22261 ttgatcaccc acatcagcgg cacgtaggcg atcccggcac ccgccgttag ccagcgcacc
    22321 agcgtcatcg gatcattagt cacaaatctc ccttgcggga tcaggcgagt cgagatccct
    22381 tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg
    22441 ctacttaaat cggcgggttt ttccggtatg ccgtattgtg tgagatagct tttcgcggcg
    22501 cacaccacca ttggcatcgc gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg
    22561 ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt cgggggctgg aattccggta
    22621 accagattgg cgctcaaacc tgggtattct ttcagcattt tggctgtcag cccggcgaga
    22681 acattttgtg ccatagttga agaacagcca atgcgtagcg tcccgatggg ggtgttattg
    22741 aaggcataca gttgctcatg aacatcctgc acttcatgaa gcatacgacg gcagccctgg
    22801 tagtaaattc taccggcttc ggtcaggcca atgctgcgtg tgctacggtt taacagcttt
    22861 acctgcaact catcttccag ttttgacacc gtctgactga tggacgaaac gctcatctgt
    22921 agctgtctgg cggcggcggt aaaagagcca aattcaacta ctttggcaaa caccgacatg
    22981 cgttttagtc gttccattat tcactctgac ttaaaagtga tttagatcac ataatataga
    23041 taacagcata acagttacgc taatatatta aatatcaatc tacagcaatg ttgctctcgc
    23101 ccggctttcc atgcccttct ctgcggcgac agatgctgaa aataataacg cctgctctct
    23161 ctatacaacc aaggtcaaca tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt
    23221 cccaccgata ttttttgaat tgcttttatc actggcgatt ttctggctgg tgcgccgggt
    23281 acttgtgcca acaggtatct acgactttgt ctggcatccg gcgttgttca acaccgcgct
    23341 ctattgctgc ttgttttatt tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac
    23401 taataagaaa attctcccgt acggccatca cggtcgtatt agtcattctg gccttcatcg
    23461 caatttttaa tgcctgggtc tattacaccg aatccccctg gacgcgtgac gcgcgcttta
    23521 gcgctgacgt cgttgcgatc gcgccggacg tttctggact cattacccag gtgaatgttc
    23581 atgataacca gctggtgaaa aaaggacaga tactgttcac catcgaccag ccgcgctatc
    23641 aaaaggcgct tgaggaagcg caagccgatg ttgcttatta tcaggtactg gcacaggaga
    23701 aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct cgcgaagaga
    23761 tcgaccaggc taacaacgta ctacaaacgg ttctgcatca gttagcgaaa gcgcaggcga
    23821 cccgcgatct ggcaaaactg gatcttgaac gcacggtgat ccgagcgcca gcagatggct
    23881 gggtgaccaa cctcaacgtc tataccggtg agtttattac tcgaggatca acggcggttg
    23941 cgctggtgaa acagaactcc ttctatgtac tggcctatat ggaagaaact aagctggaag
    24001 gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa gtgctgaaag
    24061 ggactgttga tagtgttgcc gctggggtca ccaacgccag cagcacgcgt gacgacaaag
    24121 ggatggcgac catcgactct gaccttgaat gggtgcgttt ggcgcaacgt gttccggttc
    24181 gtattcgtct cgacaaccag caagagaaca tctggcctgc gggcaccact gctacagtgg
    24241 tggtcactgg caaacaagat cgcgacgaaa gccaggattc gttcttccgt aaaatggccc
    24301 atcgcctgcg tgagtttggt taatcacgat gggtattttc tccattgcta accaacatat
    24361 tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg ctgtttgttg gctttcactt
    24421 ccagctggaa acgccacgct gggcggtact gacagcggcg attgttgccg ccggtccggc
    24481 ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc tatcgtggct ttttgcgcat
    24541 catcggcaca tttattggct gtattgccgg actggtgatc atcattgcga tgatccgcgc
    24601 accattattg atgattctgg tgtgctgtat ctgggccggt ttttgtacct ggatatcctc
    24661 gctggtacga atagaaaact cgtacgcgtg ggggctggcc ggttataccg cgctgatcat
    24721 tgtgatcacc attcagccgg aaccattgct tacgccgcag tttgccgtcg aacgttgtag
    24781 cgagatcgtt atcggtattg tgtgtgcgat tatggcggat ttgctctttt ctccgcgatc
    24841 gatcaaacaa gaagtagatc gagagctgga aagtttgctg gtcgcgcaat atcaattaat
    24901 gcaactctgt atcaagcatg gcgatggtga agttgtcgat aaagcctggg gcgacctggt
    24961 gcgacgcacc acggcactac aaggcatgcg cagcaacctg aatatggaat cttcccgctg
    25021 ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta tcgctgacgc tgattaccca
    25081 atcctgcgaa acttatctta ttcagaatac gcgcccggaa ttgatcactg atactttccg
    25141 cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc cacaagcagc tcaaacgcct
    25201 gcggagagtt atcgcctgga ctggggaacg ggaaacgcct gtcaccattt atagctgggt
    25261 cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt atcagtaaca caaaaatcaa
    25321 cgccaccgaa gaagagatcc tgcaaggcga accggaagta aaagtagagt cagccgaacg
    25381 tcatcatgca atggttaact tctggcgaac cacactttcc tgcattctgg gcacgcattt
    25441 ctggctgtgg acgggctgga cttccggcag tggtgcaatg gtgatgattg cggtcgtgac
    25501 gtcactggca atgcgtttgc cgaatccacg catggtggcg atcgacttta tctatgggac
    25561 gctggccgcg ctgccgttag ggctgctcta ctttttggtg attatcccta atacccaaca
    25621 gagcatgttg ctgctgtgta ttagcctggc agtgctggga ttcttcctcg gtatagaagt
    25681 acagaaacgg cgactgggct cgatgggggc actggccagc accataaata ttatcgtgct
    25741 ggataacccg atgactttcc atttcagtca gtttctcgac agcgcattag gacaaatcgt
    25801 cggctgtgtg ctcgcgttca ccgttatttt gctggtgcgg gataaatcgc gcgacaggac
    25861 tggacgtgta ctgcttaatc agtttgtttc tgccgctgtt tccgcgatga ctaccaatgt
    25921 ggcacgtcgt aaagagaacc acctcccggc actttatcag cagctgtttt tgctgatgaa
    25981 taagttccca ggggatttgc cgaaatttcg cctggcgctg acgatgatta tcgcgcacca
    26041 gcgcctgcgt gatgcgccga tcccggttaa cgaggattta tcggcgtttc accgacaaat
    26101 gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat gataaacgtc gtcggtactt
    26161 tggccagttg ctggaagaac tggaaatcta ccaggaaaag ctacgcatct ggcaagcgcc
    26221 accgcaggtg acggaaccgg ttcatcggct ggcggggatg ctccataagt atcaacatgc
    26281 gttgaccgat agttaagtca aaaccgacgc caaaagcgtc ggttttttca tggctatact
    26341 tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg gcaacagatg aatatttatg
    26401 cctttgattt tgatgagatt gagagtcagg aggattttta tcgtgacttt agccaaacct
    26461 ttggtctggc taaagataag gtacgcgatc tcgactcact atgggatgtg ttaatgaacg
    26521 atgtcctgcc gctaccactt gagattgaat ttgttcatct gggagagaaa acgcgtcgcc
    26581 gttttggcgc gttaatattg ctgtttgatg aggcagagga agagctggaa gggcatttgc
    26641 gttttaatgt tcgtcattag cgcaaaaaaa agcccacgaa ccgggggcaa tatcgtcgga
    26701 caaaacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt caccgctacg
    26761 agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc
    26821 acgtatatcc attggcgaag acgccacacc acttacggat acggtcccga ttgcttcacg
    26881 attttgtgct tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag agagaacaga
    26941 aagtacgctt aatgcagcta cag
//
LOCUS       DAZSIO010000041        25978 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZSIO010000041 DAZSIO010000000
VERSION     DAZSIO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25978
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.041"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACHX5T_001573"
     CDS             complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACHX5T_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR8466821.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGH"
     gene            348..641
                     /locus_tag="ACHX5T_001574"
     CDS             348..641
                     /locus_tag="ACHX5T_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR8466822.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="ACHX5T_001575"
     CDS             713..1372
                     /locus_tag="ACHX5T_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR8466823.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="ACHX5T_001576"
     CDS             1464..1910
                     /locus_tag="ACHX5T_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR8466824.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="ACHX5T_001577"
                     /pseudo
     CDS             complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="ACHX5T_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3390..3809
                     /locus_tag="ACHX5T_001578"
     CDS             3390..3809
                     /locus_tag="ACHX5T_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR8466825.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3809..5077
                     /gene="umuC"
                     /locus_tag="ACHX5T_001579"
     CDS             3809..5077
                     /gene="umuC"
                     /locus_tag="ACHX5T_001579"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR8466826.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="ACHX5T_001580"
     CDS             complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="ACHX5T_001580"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR8466827.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="ACHX5T_001581"
     CDS             complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="ACHX5T_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR8466828.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7561..8280
                     /gene="fadR"
                     /locus_tag="ACHX5T_001582"
     CDS             7561..8280
                     /gene="fadR"
                     /locus_tag="ACHX5T_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR8466829.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8332..9864)
                     /locus_tag="ACHX5T_001583"
     CDS             complement(8332..9864)
                     /locus_tag="ACHX5T_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR8466830.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10194..11492
                     /gene="dadA"
                     /locus_tag="ACHX5T_001584"
     CDS             10194..11492
                     /gene="dadA"
                     /locus_tag="ACHX5T_001584"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR8466831.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11502..12572
                     /gene="dadX"
                     /locus_tag="ACHX5T_001585"
     CDS             11502..12572
                     /gene="dadX"
                     /locus_tag="ACHX5T_001585"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR8466832.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12740..14154)
                     /locus_tag="ACHX5T_001586"
                     /pseudo
     CDS             complement(12740..14154)
                     /locus_tag="ACHX5T_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14765..16501)
                     /locus_tag="ACHX5T_001587"
     CDS             complement(14765..16501)
                     /locus_tag="ACHX5T_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR8466833.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="ACHX5T_001588"
     CDS             complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="ACHX5T_001588"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR8466834.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17610..18221
                     /gene="emtA"
                     /locus_tag="ACHX5T_001589"
     CDS             17610..18221
                     /gene="emtA"
                     /locus_tag="ACHX5T_001589"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR8466835.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18223..18957)
                     /locus_tag="ACHX5T_001590"
     CDS             complement(18223..18957)
                     /locus_tag="ACHX5T_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR8466836.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19158..19412
                     /locus_tag="ACHX5T_001591"
     CDS             19158..19412
                     /locus_tag="ACHX5T_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR8466837.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19462..21416)
                     /locus_tag="ACHX5T_001592"
                     /pseudo
     CDS             complement(19462..21416)
                     /locus_tag="ACHX5T_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            complement(21442..22296)
                     /gene="modD"
                     /locus_tag="ACHX5T_001593"
     CDS             complement(21442..22296)
                     /gene="modD"
                     /locus_tag="ACHX5T_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFR8466838.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22296..23105)
                     /locus_tag="ACHX5T_001594"
     CDS             complement(22296..23105)
                     /locus_tag="ACHX5T_001594"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR8466839.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23115..23873)
                     /locus_tag="ACHX5T_001595"
     CDS             complement(23115..23873)
                     /locus_tag="ACHX5T_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8466840.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23870..24850)
                     /locus_tag="ACHX5T_001596"
     CDS             complement(23870..24850)
                     /locus_tag="ACHX5T_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFR8466841.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24850..25872)
                     /locus_tag="ACHX5T_001597"
     CDS             complement(24850..25872)
                     /locus_tag="ACHX5T_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFR8466842.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6489 a         6381 c         6737 g         6371 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgcga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcttgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc tgcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgttttatct
     3001 gcaacgattt cagtcataat cattcgcctc tttaaatata taaattgtaa tgaaactcct
     3061 gttttacaac tattaataaa ttttacttca tctaattcat agttagccgg gcgggatgcg
     3121 tcaatgtctt tatttctatt aatatgataa atatcaaaca atgtttaatg tcattatggc
     3181 gaatgcttct attctatttt tttgccgggt gatatttttc atttctgctg gatgagcgtc
     3241 gtcgccagaa ggccacgtga gcacaggata agagaacgaa aaatcagcag tctatgcagc
     3301 gacaaatatt gatagcctga atcagtattg atctgctggc aagaacagac cactgtatat
     3361 aaaaacagta taacttcagg cagattatta tgttgtttat caagcctgcg gatctccgcg
     3421 aaattgtgac ttttccgcta tttagcgatc ttgttcagtg tggctttcct tcaccggcag
     3481 cagattacgt tgaacagcgc atcgatctga atcaactgtt gatccagcat cccagcgcga
     3541 cttacttcgt caaagcaagt ggtgattcta tgattgatgg tggaattagt gacggtgatt
     3601 tactgattgt cgatagtgct attaccgcca gccatggtga tattgtcatc gctgctgttg
     3661 acggcgagtt tacggtgaaa aaattgcaac tacgcccgac ggtacagctt attcccatga
     3721 acagtgcgta ctcgcccatt accatcagta gcgaagatac gctggatgtc tttggtgtgg
     3781 tgatccacgt cgttaaggcg atgcgctgat gtttgccctc tgtgatgtaa acgcgtttta
     3841 tgccagctgt gagacggtgt ttcgccctga tttatggggt aaaccggtgg ttgtgctatc
     3901 gaataatgac ggttgcgtta tcgcccgaaa cgctgaggca aaggcgctgg gcgttaaaat
     3961 gggcgatccc tggttcaaac aaaaagatct gtttcgtcgc tgtggtgtgg tttgctttag
     4021 cagcaattat gagctttacg cagacatgag caatcgggtg atgtcgacgc tggaagaact
     4081 atcgccccgc gtcgagattt acagtattga tgaagcattc tgcgatctga caggtgtgcg
     4141 taattgtcgc gatctgactg attttggcag agaaattcgc gcaacggtgc tacaacgtac
     4201 ccatcttact gttggtgtgg ggatcgccca gaccaaaacg ttggctaagc ttgccaatca
     4261 tgcggcaaaa aaatggcagc ggcagacggg tggggtggtg gatttatcaa atctggaacg
     4321 ccagcgtaaa ttaatgtctg ctctccccgt ggatgacgtc tgggggattg gacggcggat
     4381 cagcaaaaaa ctggacgcga tggggatcaa aaccgttctc gatttggcgg atacagatat
     4441 ccggtttatc cgtaaacatt ttaatgtcat gctcgaaaga acggtgcgtg aactgcgcgg
     4501 cgaaccctgt ttgcaactgg aagagtttgc accgacgaag caggaaatta tctgttcccg
     4561 ctcgtttggt gaacgcatca cggattatac gtcgatgcgg caggccattt gtagttacgc
     4621 tgcccgggcg gcggaaaaac ttcgcagtga gcatcaatat tgtcggttta tctccacatt
     4681 tattaagacg tcaccatttg cgctcaatga accttattac ggcaatagcg cgtcggtaaa
     4741 actgctgacg cccactcagg acagcaggga tatcattaac gctgctacgc gatctctgga
     4801 tgccatctgg caagcgggcc atcgctacca aaaagcgggc gtgatgctgg gggatttctt
     4861 cagtcaggga gtcgcgcagc tcaatttatt cgatgacaac gcaccgcgcc ccgggagtga
     4921 gcaattgatg gcggtaatgg atactctgaa tgctaaagag ggcagaggaa cactctattt
     4981 tgccgggcag gggatccaac aacaatggca gatgaagcga gctatgcttt caccacgtta
     5041 tacaacgcga agttctgatt tactgcgggt caaataaata tagcggcagg aaaaaagcgc
     5101 tcccgcagga gcgccgaatg gattagcgac cgaacagatc acgttttttc gctttaaacg
     5161 gctgggaaat caccaccagc accgcgacaa tcaggtaagc gataaaaata ccgagcagcc
     5221 actgcggcat ttccatacct aaaaaatccc actgacgctc ggcgcaatcg ccagaggcga
     5281 caaacacttg cggcacccat ttatccagcg gcagccattc cgggaaacga accataaaat
     5341 cacaggtggc aaatggcgaa ggatagagct gaagcatggt gtgctcgtaa gttaactgca
     5401 caccgcggaa cgcactatac aaccagataa ccatcgctac ataacgcagc ggagttttcg
     5461 gggcgatcgc gccaatcagc gcagcaccca gaacgccgaa taacgcgcag cgttcataaa
     5521 tacagagcac gcaaggtttc agtaacatca catgctggaa ccacagcgcc gtcagttcca
     5581 gtgccagagc agtaaacgcc atcaacagcc acgcgccccg gccttgtgaa cattggttca
     5641 aaaatcgcaa cataatcatt tccctgcaat atgcatagag tgcgcagttt aaaccaattc
     5701 attcgctgcg ccaccaggag gcagcaaaaa taggacgcaa ttggacaatt atcgggcgaa
     5761 aaggcaaacc gggcatttat atgcccggta agttgttatc aaagcgttgc tatccagccc
     5821 atttgcataa accattcggt tacaggggca agcgtaaact cgacgcagag caagccgacg
     5881 agtgtcagta cgagggtgta aggcagggcc atccacacca tgcggccata agagaggcga
     5941 atcaatggcg cgagtgcaga ggtcagcagg aacaggaacg cagcctgacc gttcggcgta
     6001 gcgacagagg gcagattggt accggtatta atggcgaccg ccagcagctc gtattgcttc
     6061 aacgtgatag cgccactttc cattgccgct ttcgcttcgt tgatataaat cgtccccacg
     6121 aagacgttat ccgaaatgga tgacagcaga ccgttgaaaa tatagaacag cgacagctga
     6181 gcatgttcag atgcctgcaa cacaaactga ataatcggcg aaaacagttg ttggtcgata
     6241 atcaccgcga ctaccgagaa aaacaccgtc aacagtgcgg tgaatggcag agattcggtg
     6301 aaggctttac cgatagcatg ttcatcggtg acaccggtca atgatgttgc cagaataatg
     6361 actgacaaac caatcaagcc aacttccgcc agatgcaacg ccagcgcagt caccagccag
     6421 acgccaataa tcgcctggac aatcagacgg attttatcct gacgggtacg ctggtggcgg
     6481 ctttgatcgt caaactgttg caacacttcg cggactttct ccggcagcgt ttcaccgtag
     6541 ccaaaccaac gcagcttctc taccagcagg caggttaaca ggccacaaat cagaaccgga
     6601 acggtcaccg gcgacatgcg caggaagaaa tcgccaaaat gccagccagc cgctttagcg
     6661 atgatcaggt tctgtggttc gcccaccatg gtcattacgc cgcctaatgc ggtgccgaca
     6721 ccggcatgca tcatcaggct acgcagaaag ccacggaact gttccagaac cacgttgtaa
     6781 tgcttgtcga tatgactatc gtcttgcagg tcggtgtctt cggtacggga agaggctacg
     6841 cgatgataaa taccataaaa accgactgca acgctgatca ccaccgccac gacggttaag
     6901 gcatcgagga acgcggagag gaacgctgcc gccacgcaaa aagagagcga cagcagcatt
     6961 ttggagcgaa tgcttaacag caaacgggta aatatgaaca gcaacagctg tttcataaaa
     7021 tagatacccg ccaccataaa catcaataac agcaagactt caagatttgc cgccacctct
     7081 tcacggacgt gttccgcgct ggtcatgccg atgaataccg cttcgatagc caacagacca
     7141 ccggggagta gcgggtagca tttcagggcc atcgccagag tgaaaataaa ttccgctacc
     7201 agcaaccagc ccgcgacgaa agggctgatg aggaaaatta acgggtttac gattaagaaa
     7261 atgatgaggg cgagtttgta ccagtcgggg gactggccca aaaaattgcg ccatagcgcg
     7321 cggccccagg agatctccat gatggtttcc cttaccttac aaataatcaa tgatgttttt
     7381 atgtttaaac gcaaagctta acggtcaggc aggagtgagg caagtcttga tagtcaaggg
     7441 gaaagagatg cggaaaatga agccttgatc cctttttctt ctttttgtct gctatcagcg
     7501 tagttagccc tctggtatga tgagtccaac tttgttttgc tgtgttatgg aaatctcact
     7561 atggtcatta aggcgcaaag cccggcgggt ttcgcggaag agtacattat tgaaagtatc
     7621 tggaataacc gcttccctcc cgggactatt ttgcccgcag aacgtgaact ttcagaatta
     7681 attggcgtaa cgcgtactac gttacgtgaa gtgttacagc gtctggcacg agatggctgg
     7741 ttgaccattc aacatggcaa gccgacgaag gtgaataatt tctgggaaac ttccggttta
     7801 aatatccttg aaacactggc gcgactggat cacgaaagtg tgccgcagct tattgataat
     7861 ttgctgtcgg tgcgtaccaa tatttccact atttttattc gcaccgcgtt tcgtcagcat
     7921 cccgataaag cgcaggaagt gctggctacc gctaatgaag tggccgatca cgccgatgcc
     7981 tttgccgagc tggattacaa catattccgc ggcctggcgt ttgcttccgg caacccgatt
     8041 tacggtctga tccttaacgg gatgaaaggg ctgtatacgc gcattggtcg tcactatttc
     8101 gccaatccgg aagcgcgcag tctggcgctg ggcttctacc acaaactgtc ggcgttgtgc
     8161 agtgaaggcg cgcacgatca ggtgtacgaa acagtgcgtc gctatgggca tgagagtggc
     8221 gagatttggc accggatgca gaaaaatctg ccgggtgatt tagccattca ggggcgataa
     8281 tcccttccgt ttaaagagca accccctcaa acgaggggtt ttttgttgtt tttacagatt
     8341 tcccattctt ggcgggcaac gttccagcaa ctcgatgctg ccgtcttcgt tttgctgttc
     8401 gagcatcaca tcaaatcccc acaggcgatg cacatgcttc aggacttctt tgcgcccccg
     8461 atccagcggt gcgcgattat gtggaatata acgcagcgtc agcgaacggt cgccgcgcaa
     8521 atccacgttc cagatctgaa tattcggctc cagattactt aagttatatt gcgacgataa
     8581 ccggttacgg atctcccgat aaccttcttc attatgaata gcggaaatct ccagataatt
     8641 atgccgatca tcatccagca cggtgaagaa gcggaaatca cgcatcactt tcggtgacag
     8701 aaactggctg ataaaactct catctttgaa atcacgcatc gcgaaatgca gcgtttccag
     8761 ccagtcggaa ccggcgatat ccgggaacca gtatttgtct tcttccgttg gcgactgaca
     8821 aatccgttta atatcctgga acatggcgaa cccgagggca tacgggttga tgccgctgta
     8881 ccacgggctg ttataggggg gctggaagac cacattggtg tggctgtgca aaaactccag
     8941 cataaaacgt tccgttactt tcccttcatc atacagatgg ttaaggatgg tatagtgcca
     9001 gaaggtcgca cagccttcgt tcatcacctg agtctgtttt tgcggataaa aatactggct
     9061 caccttacgc acaatacgca ggatttcacg ctgccatgat tccagcagtg gcgcattttt
     9121 ctccataaaa tagagcaggt tttcttgtgg ttcggacgga tagcggcgcg cttcagcaac
     9181 cgttttctct tcctcgcgct tcggcagggt acgccagagc atattgacct gactttgtag
     9241 atactcttcg cgacttttct gccgggcttt ctcttcttgc agcgagattt tttgcgggcg
     9301 tttgtaccgg tccacgccgt agttcatcag cgcgtggcac gagtccagaa gccgttctac
     9361 ttcatcaacg ccataacgtt cttcgcactc ggtaatatat ttacgggcaa aaatcagata
     9421 atcgacaatc gaactggcgt cggtccaact gcggaataag caattgtttt taaagaaaga
     9481 gttatgtcca tagcaggcgt gtgccatcac cagcgcctgc atagtaatgg tgttctcttc
     9541 catcaagtaa gcgatacacg ggttagagtt aatgacgatt tcataggcca gcccttgctg
     9601 accgtgctta tacagccgtt cagtctcgat aaacttttta ccgaatgacc agtgcggata
     9661 gttaattggc atgccgacgc tggaataggc atccatcatc tgttctgagg ttatcacttc
     9721 aatctggtgc gggtaggtat ccagccggta gagtttcgcc acccggtcta tctctgccag
     9781 ataaacatcc agcaggtcga acgtccagtc gggtccatcg ctcaaacgtg tggtgtcctt
     9841 attcatagaa tcgatcgtcg ccatacgcgc acctcattgt tgtcggcgct ctctgtgtgg
     9901 agcacctcat ttcaagcata gaacacctgt taaaaaccgc gtcgccggag aatttttttc
     9961 tttgcgattt cttattatca gagtgccact aatccgcttc tgaacggaat tttatgctgg
    10021 ataaaaaggg cgttcagcag gagatactaa agacgccata ttgccgcaga gtcagggaga
    10081 tgtgagtcag ctcaccataa aaaagccgta tgttgaataa tattttcaac tgagttatca
    10141 agatgtaatt agattattat tcttttactg tatctaccgt tatcggagtg gctatgcgag
    10201 ttgtcatact gggaagtggt gtggtaggcg ttgccagcgc ctggtactta aatcaggcag
    10261 gacatgaggt caccgtcatt gatcgggagc cgggggccgc cctggaaacc agtgccgcga
    10321 atgccgggca aatctccccc ggatatgctg caccgtgggc agcaccaggt gtgcctttaa
    10381 aagcgattaa atggatgttc cagcgccatg cgccgctggc ggttcgtctc gacggtacgc
    10441 agtttcagtt gaaatggatg tggcaaatgt tacgtaactg cgacaccagc cactacatgg
    10501 aaaacaaagg gcggatggtg cgtctggcgg aatacagccg tgattgcctg aaagcattgc
    10561 gcgccgaaac caatattcag tatgaagggc gtcagggtgg gacgctgcaa ctgttccgta
    10621 ccgaacaaca gtatgaaaat gcgacccgcg atatcgccgt gctggaagat gccggcgtac
    10681 cgtatcagct gctggaatcc agccgcctgg cggaagtgga tcccgcgctg gcagaagtag
    10741 cgcacaaact gacgggcggc ctgcagttac ccaatgatga aaccggagac tgccagctgt
    10801 ttacccagaa tctggcgcgg atggcagagc aggcgggggt taaattccgc tttaatacgc
    10861 ccgttgacca actgctttgc gacggcgagc aaatctacgg cgtgaagttt ggcgatgaag
    10921 tgattaaggc cgatgcgtat gtgatggcgt ttggttctta ctcgacggcg atgctcaaag
    10981 gcattgttga tattccggtg tatccgctga aaggctactc gctgactatt ccaattgcgc
    11041 aggaagatgg tgcgccagta tccaccattc ttgatgaaac ctacaaaatc gccattaccc
    11101 gtttcgataa ccgcattcgt gttggcggaa tggcggaaat tgttggtttt aacaccgagc
    11161 tgttgcaacc gcgtcgtgaa acgctggaaa tggtggttcg cgatctctat ccacgcggcg
    11221 gtcatgtcga gcaggcgact ttctggacgg gtttgcgccc gatgacgcca gacggtacgc
    11281 cggttgtcgg gagtacacgc tttaaaaatc tgtggctgaa taccggtcac ggcacgctcg
    11341 gctggacgat ggcttgcggt tccggtcagt tgttaagcga tctgctctct ggtcgcacgc
    11401 cagcgatccc atatgaggat ctaagcgtag cgcgctacag ccgtggattt acgccatcac
    11461 gtccgggcca tttacatggc gcacacagct aaggaaacga gatgacccgt ccgatacagg
    11521 ccagcctcga tctgcaggca ttaaaacaga atctgtccat tgtccgccag gccgcgccgc
    11581 acgcgcgcgt ctggtcggtg gtaaaagcga acgcttacgg gcatggtatt gagcgtatct
    11641 ggagcgcgct cggggccacc gatggctttg cattacttaa cctggaagag gcaataacgt
    11701 tacgtgagcg cggctggaag gggccgatcc tgatgctgga aggatttttc catgctcagg
    11761 atctggagat ttatgaccag caccgcctga ccacctgcgt acacagcaac tggcagctca
    11821 aagcactgca aaatgcgcgg ctaaaagcac cgttggatat ttatcttaaa gtgaacagtg
    11881 ggatgaatcg gttgggcttc cagtccgatc gcgtgcttac cgtctggcag cagttgcggg
    11941 caatggcgaa tgttggcgaa atgaccctga tgtcgcattt tgctgaagcg gaacatcctg
    12001 atggaatttc cggcgcgatg gcgcgtattg agcaggcggc ggaggggctg gagtgtcggc
    12061 gttcgttgtc caattcggcg gcgactctgt ggcacccgga agcgcatttt gactgggttc
    12121 ggcctggcat tattttgtat ggcgcttcgc cgtccggtca gtggcgtgat atcgccaata
    12181 ccggattacg tccggtgatg acgctaagca gtgagattat tggtgtccag acgctaaaag
    12241 cgggcgagcg tgtgggctac ggcggtcgct atactgcgcg cgatgaacag cgaatcggca
    12301 ttgtcgccgc aggatacgcc gacggttatc cgcgccatgc gcctaccggt gctcctgttt
    12361 tagtggacgg cgtgcgcacc atgacggtgg ggaccgtctc gatggatatg ctggcggttg
    12421 atttaacgcc ttgcccgcag gcggggattg gtacgccggt tgagctgtgg ggcaaggaga
    12481 tcaaaattga tgatgtcgcc gccgctgccg gaacggtggg ctatgagttg atgtgcgcac
    12541 tggcgttacg cgtcccggtt gtgacagtgt aacttgttgt aagccggatc ggaggcagcg
    12601 tcttctggtt gcgaaaaaat catccatccg gctggtcagc aactgtagtt gttaatgtga
    12661 cagagccatt gcccatgata gtgtccatta aaagaatgga cactatttcc ccggaacctg
    12721 aactcgccgc acaggcgttc tacataaaac gcttacgctt cattgttgac tcggagtcga
    12781 cttcagataa atcacgcttc acccttgatg cagattcggg ttcatgttca gattgttgat
    12841 taacaggctg cgagtcctgt tgtctttcca gactcaacag gcccaactgt tgatccagga
    12901 gctccaggtc ctcctgtacc ttcacgcgct ccaggagttc cgggtcatcc aggaactcca
    12961 gatccataag gtcctccagc tcctcgagtc cttccaggtc aacctagcct tccttgtgca
    13021 aaaagtccaa aaggtccttc aggccctgct gttttttcag gccctcgacg gctttgagca
    13081 gcgtcggtaa attgtctttg tcccacgggt taccattaca gtcaaacacc ttcaggctct
    13141 ggggctgcgg cacctgtacc gttttaaact tgttttctcc acagtagtaa attattagtt
    13201 tgggcggcag ccgtggtgaa gagatcaaat cgttactaga acagtcaaat actttcaggc
    13261 gagtcaagtt ctccagcatc tcatcaggta ttgagttaat attgttgccg gcacaggata
    13321 gtttatacag gtactggggc atcgacggcg gtaaaaattg caagaagttt tcagaacagt
    13381 taatcactct caggttagtc gacagctcag gcagccagat aatactgttt tcatagcact
    13441 tcagtaccat cagttttgag ggctgcacct tcggtaacaa gatcaagacg ttatctgtac
    13501 agtcaagcac cagcagccac tttggcatca ccttcggtaa cgcggtcaac acgtttttac
    13561 tgcagtaaaa ttcaatcaga tgtggaggca aattctcagg gagtgaagtc aatcccaggt
    13621 tgctaagatt gagcatactc cgtcttgtaa tgagacattc cttcattctg cgcaatgctt
    13681 ctgcccggtt ttcaatccgg cttgctggca ggttttcttc caggatattt tccttgattt
    13741 cttccagact ttctttcagg tcttcttggg gaatttctga ctgtttgttt tgctggtttt
    13801 cttccaggat ttcgtacagg tttttttgca ggttttcttg caaaccatcc tgctgtatag
    13861 cccattcgca ccatttggcg accatcatga actcgttatg ttgctgattc agacgagtcc
    13921 ccaacgtgat gatggtattg ttgtccacct gtatgttcac tttgcccgcg atactgaggt
    13981 caatgatctg ccagggggca tcaccccagc tgaatttttg cgggctgaga tacttcatgt
    14041 tctgcaaaat ttgattcttg agaagagccg ccctggcggg cgtcgtacgc ctgggttaac
    14101 ggaagatggg ggaatggcgg gggcgaatgg gcagatgtct ttaaagctag ccataatgct
    14161 acgtttcctt gaaataaata tcctctggca gcgccggaga cgtgaatgtg gtcaccgcaa
    14221 aggagccaca ggacgtcatt tgcgctgata tcactactca tcgttgactt ttttcctggt
    14281 cagaaatgcc gtgtcgggtc tggtacatgc tgtattacgg cctaggtagc tctgacgtat
    14341 taaagaaaat acgagagtga acgacccgga aataatcgtt attaagcaaa ggaataacta
    14401 taagaaatcg ctcatgcatc cggtgctaca ggcggatgaa acgcctattg tgtgggctat
    14461 gtcagtgcgg caggttgcgt gggggaatat gttcaggcga tgtcaggcag gggcccctga
    14521 tggttgaccg gtaatgcggg gctggttttt attacctgtt acagtcggaa aaagccctta
    14581 cgctgacttt cttgagccgg tgttttgagt tcattagcca gagcctgcaa tggtttgtca
    14641 tccataattt aaccttcttc tgatgttggg atgagcatat caaaatcagg aaatgacaag
    14701 tttattacag gctcaggctg cgttcagtaa agcatcgcat ccggcgatag atgctgcatt
    14761 aaatttaaga ttcagcttcc tcttcagcca cccgaacgcc aatcttcaac acttcattat
    14821 cttctttctc ggccaccgtc cagatcatcc cggcaaactc tacctggtca ccgacaaccg
    14881 gtgccgcgcc tagcaactgc tggacaattt cacccagcgt ttgctgttta tcacgatatt
    14941 ctcgcccgtc ttccaggcca tatatcagcg caacatcagc atatttggcg ctggcttcga
    15001 gaatgaagtc accaaagaag cgttgatcca gcgcgaccgg tggcgactgg ctgaacagtt
    15061 taccgagcgc cgggagatcg cgttcccgac caattacaca caatacatcg ccttcgcgca
    15121 gtcgggtgct gccggtagga tgaagcaact ggttatcacg aaacagggct gcaatacgcg
    15181 tctcttttgg catatgcaaa tcgcgcagtg ccgcacctac acaccattta tcagcactca
    15241 attgataaac aaactgctcc cacggatttt ccggatgaat atccaggcca acgcgtgaca
    15301 ccggacgtcc gacgggcgga accaccactt tggctttttt cgccgcccac gagagtgatg
    15361 ttccctgcaa gagcagtgaa accagaacca caaagaaggc gacattaaag aacagtcgcg
    15421 cattctccag ccccgccatc atcgggaaca cggctaggat gattggcacc gcgccgcgta
    15481 atcccaccca gctgataaac acgcgctcac gcagattgaa accgcggaaa gggagcaatc
    15541 cagcaaatac cgaaagggga cgggcgaaga atatcatcca tgccgacaaa atgagcgccg
    15601 gaatggcaat aggcagcaga tcgcttgggt taaccaatag ccccagcacc aggaacatgg
    15661 cgatttgcgc cagccaggcg aggccgtcga aattttgcag gatgccgtag cggttgcgaa
    15721 tcggccgatt acccagcaga aaaccgcaca gataaacagc cagaataccg ctgccttcca
    15781 gcgccgtggt taaagcaaaa attaaaatac cgccgcttaa tgccagcaat ggatataatc
    15841 cggcaggcag ggcgatgcga ttaatcattt gcagcagtaa ataaccaccg ccaagaccga
    15901 tgacaatccc gaggccaaat tgctgcagaa tatcgaccac gaacatccaa ctgacactgc
    15961 tttcatgctg ctggatcatt gcaatcaggg taatcgtcag aaagaccgcc attggatcat
    16021 tactgccgga ttcaatttcc agcgtcgagc caacacgttc gttaagcccc ttaccaccca
    16081 gcagagaaaa gaccgctgca gcatcggtgg agccgacgat agcgccgatt aataagcctt
    16141 caatcaaatc aagattaaac agccacgccg ccatcatgcc ggttaaacca gaggtgataa
    16201 gtacgcccag cgtcgccagc gacagcgccg gtcctaacgc cacacgaaag gaactggcct
    16261 gagtgcgcat cccgccgtcg agcaaaataa tcgccagcgc caggttactt accatgtagg
    16321 cgaaggggta attatcaaac gggataccgc cgacgccatc gactcccgcc agcatgccga
    16381 tcgccagaaa aataaccaga ataggaatgc caagacggga agaaaatgaa ctaagtaata
    16441 tactgctggt gacaaggatg gatcctaaga tgaaaaggct aattattgtt gtggcatcca
    16501 atggtcggtt actcctgatt acgctgtctc ttatataaac cctaccatat tagcggcaga
    16561 gacagcgttt tacttagccc tgaagcgtga tttttttaca ttttaagaac aggatgaccg
    16621 ctaatcgtca actgagcgcc ttcccgggtg ttattcagaa tggcatgtgc gcccaacggc
    16681 agcgtaaccg tgcgttgttc atgaccaaaa tcgaggccgg taatgagtgg aatcgacagg
    16741 cgggaacgca aaaacgcgta cactgactcc aggttgtaac ctgcgtcata atcattgggc
    16801 gtgctgccgc taaagctacc gagaataatc gccttctgac gtggcaaaat tcccgcatga
    16861 tagagctgca acagcatacg ttcgacgcgg aaagggtgca cgttaatatc ttccagcacc
    16921 agaataccgt tctcaatttt tggcatccac ggtgtaccaa tcagtgaaat cagcatcgca
    16981 agattgcctc cccacaacgt gccttcggtc tgacatgtcg ggccttcccc ttgccattca
    17041 atggtgaagg tttcattgcg taacgccagc cagaaatggt gctcggtaaa ggcgttcagt
    17101 tcatccgcac caaagtttgc taccagcatg gggccgctaa aggtgatgac attgccttgc
    17161 gccagaagac cgcactgaat ggcggtaaaa tcgctatgtc cgcaaatgag taacgggtca
    17221 tgttgttggc gagccaccag tgcctgccag tcaatatctg ccagtaaacg actggcaccg
    17281 taaccgccgc gtacagccag cacgatggtg ttgggggtag tcagtctggc aagggaattg
    17341 agatcttcca gacgctccgt ttccgtaccc gcaaaacgct cacaacgacg ggcaataacc
    17401 tcgacgttat tgacttgatg ccccgcgtcg gtaaggcgct ggataccacg cagcgcggcg
    17461 tgctgtttaa tgctgtaacc cgatggggca attaagtgaa acagagacat ggcaattcct
    17521 tgctgacaac agaaacgaaa tgtatatcat gccgcttagg tgtgccgttg tcacctcaac
    17581 ggcgattcca ggctataagg atagaagaag tgaaattgag atggtttgcc tttttgattg
    17641 tgttattagc gggttgttca tcaaagcatg actatacgaa cccgccgtgg aacgcgaaag
    17701 ttccggtgca acgtgcgatg cagtggatgc caataagcca gaaagccggt gcagcctggg
    17761 gcgtcgatcc acaattgatc acggcgatta tcgctatcga atcgggtggt aatccgaacg
    17821 cggtgagtaa atcgaatgcc attggtttga tgcagttaaa agcttcaacc tccggacgtg
    17881 atgtttatcg ccgtatgggc tggagtggtg agccgacgac cagcgagctg aaaaatccag
    17941 agcgtaatat ttcaatgggg gcggcttacc tgaatattct ggaaaccggc ccgctggcag
    18001 gcattgaaga tccgaaggta ctgcaatatg cgctggtggt gtcatacgct aacggggcag
    18061 gtgcgctgct acggactttc tcgtcagatc ggaaaaaggc gatcagcaaa atcaacgatt
    18121 tggatgctga cgagttcctc gatcacgtag cgcgaaatca tcctgcgccg caggctccgc
    18181 gctatatcta caaacttgag caggcactgg acgcgatgta aatcagtcgc gcactttgtc
    18241 cgctttttcc cgggcttctc gctcgagaga gaaaataatc cgctgtaatt gccgctccac
    18301 cgtcgggctg acgttaagaa aacggaagct cagacgggga gtggtgatgg tttcattctt
    18361 gccatcaatc actttgcgct cgctggtgga gattaactgg gcgtcaaagt gaaaaacacc
    18421 ccattgcccc atgttgactt caatttgagc gaagcgcata ccttcttgta attcggcagg
    18481 ctttgctgtt tccagtaatg cgcccatgcc gcctaacgac aaatcataca agcggaaacg
    18541 taacgtactg ttatccgcca gtttggtctg gcaaaaataa ggcggatgga gtggggcgga
    18601 gatgcggaaa tatcggcgtc gttgtacaaa ccataaggtg ggaggcggta cggtaataaa
    18661 tgccggaagc tgcaagtatt cactctgctg tagttgttca acagtaaact cgactttcgc
    18721 accctgagtt tcggcggtaa tggtaatgtg ctgtgccttt agcacggcga tgttgtcttc
    18781 ggcttgactg ccgaaatcca gcaccagttt atccggggtt attgccagta ttttgctgat
    18841 cagctgcccg ccattccaac tgagacgcaa aggaattgcg gctttgtgca aatcgcgtaa
    18901 cacgcccagg acggctaacg gattttgttt caggaactgc tcatggtaat gactcacgcg
    18961 gaaaaactcc tgactgacaa actgtctttg gtttatcggt catagttaac aaaacttaat
    19021 acaaatgcgt gaatattttt tacatgttgt tcttaaatca gccgacaaac gcccagcatc
    19081 gatcccgccc ttgcctatac ttagagcgtt gacgtaagca tttcttgcga ccgattcatc
    19141 gaaacgaggg cctgaacatg ggaattattg cctggattat ttttggcctg atagccggca
    19201 ttatcggcaa gctaatcatg ccggggcgtg atggtggtgg atttttcctg acctgtattc
    19261 tcgggatagt cggtgcggtg gtcggcggct ggctggcgac catgtttggc attgacggct
    19321 ccatcagcgg ttttaatttg cacagcttcc tggtggcggt ggtgggcgct atcctggtgc
    19381 tgggcgtatt ccgcctcctg cgaagagaat aagattttca taaggcggat agcgatacag
    19441 atgccgctat ccgctttcac atcagaacgt atactcgaca cctgcccaga aattgcgacc
    19501 ttcttcaata aacccttcac tgtaggcata ttgggtatca aacaggttat tgaccgacgc
    19561 attgacgctg aagccatgac ctaaggtgta atcggctcga atgtgggtca ccgcaaaacc
    19621 ggcggctttt tgtgaaccgt cactgttgct gtagctggag gaacgcgcct cttccgacag
    19681 cgttacgctt aacggctccc atggtttgag tgtcatccat gcggtcattg tctgcgttgg
    19741 aagatcggtt atcttgccga tgtctttgcg tttggcgtca gcgtggatca gagcgtagct
    19801 caatcctaca tccagtatat tgctgatttt ccccttaata ccggcatcca gaccgctgta
    19861 atccaccgtg ccgctgttct gattttgaat ggtatcggca ttgatattgt gcgagaggat
    19921 ggcatcactc acccggttat agtaaacgct gacctcaaat ccccagtcgc gcgtgaaggc
    19981 accattccag gttaaatcca ccccgcgagc gcgttccggc ttgagctgcg ggttaacaat
    20041 cgctatctgg ttatacgcag gtttggatgt ggtatagcgt tctttcagcg tcggaaagcg
    20101 tgtgcggtca tagtacgaaa gcgccagagt gtcttcattg gcaaagtggt atttccccat
    20161 cacctgccag ttaaaagctg actgattgtt gtcgtcataa tgggtgatgc tgccatcttt
    20221 ctcgtgtttt ttcgcttcta cgctatcgcg ccagtcatag ctgattcccg ccacgacatc
    20281 gacattatca gcagcagccc attgatattc actggcgagc gaccaggtac gatcttcata
    20341 gcgatcgtaa gcggcgtgcg gcgcaccttt ttcccggtgt acgtcatctt tccagttaac
    20401 ggcaaacgac agcagatcgt tttcacgcac atcggctgcc agttgtaatc cggcaccgtc
    20461 gctgtaatcg gaataatggc tgtagctgcc ttttttattt ttcaaatcag ccagcgagtt
    20521 gtacatcatc agcgtatttt caaaggtgtc gcgatacagc cgacttttca gggtaaaacg
    20581 gtcgtttagt tgggtcgctc cctgataata aaaactttct ttgtcatact ctggccactg
    20641 ccagtagcgt gatttttgac cacaatttcc gctgtatggc gggttatctt tttcaccatc
    20701 ctgcttaatg taagtcagcg tgtattcatc gttttcgcgc ggtgtaaaac cgagcttcac
    20761 aatgccgcgt ttatcatcag ccgatgaatt aatcatcttg ccgtgtttgc ctgcaatatc
    20821 attatttaca ccatgcggca ggccgagaaa atcctgcttt agctggctac cgctgacttg
    20881 caaataccca agatcgctgc tggcggcaaa tgaagcatgc atatcgtagg cattgtcctg
    20941 actacggctc catccctggc gatatcccag acttgcttct tcagggtggt gatattaatg
    21001 gctccgccca tctgattagg cccatgaagc agcgacgaat accctttgga aacttcgact
    21061 gcccccagat tgttggtcag aatccgcgcc agatcgaggt tgccgtcata ggggacataa
    21121 atgggcacac cgtcgaaata gaccggcacc tgccgactat caaagccacg aacttttacc
    21181 tgttcttcgt tgcggctgcc tgacttttgc agcaccacgc cggggacgac acttaatgcc
    21241 tgagcgacat tctgtttatc caaggccttc atggtgggtt gatcgagaac ggtggtcgtc
    21301 gtcgatgata ccggactgga ccatacggtc agcgtgtcgc tctcctgagc cgctacggcc
    21361 tgctgggtaa aggcaagtga taatgctgtg cagagataac gttttttgag tctcatagaa
    21421 taaattccgt ttaacaaaga attaaataga ggctgtgggt tgcagactga ctttaatgtc
    21481 agctggtgcc gcgtaataag gcgcggaggt aataaaaagg cgaatgccgc agtcgaggta
    21541 atttttgagt gtcgtcagat taattccgcc ggtcagagcc agcgtgcagt gaggagccag
    21601 cgacggcgct atttgcgcaa tttcagttgc ctgttgcgga ctaaatttgt cgagctgaag
    21661 tacgtctggt tgtgcgcgta acgcggcgat agcctctttc ggcgtgtcgg cttcaacaac
    21721 aattttcttc tcgggtgcgt ggcgacgtaa ctgagcgatt gcgcctgacc agccctgatt
    21781 gtcatgaaga aaatggcggt ggttggcaaa caataatatg gtttccgcac atccggcacg
    21841 atgaattagt cctccggcag ccagaattgc ctgcgaagcc agtaagcggg tgcccggaat
    21901 tgcttttcgg gtgcaggcga tattgccgtc aggataacgt tcacgaagca atgccagcat
    21961 ttgagcgaga taatcagaaa caccgcaact ccactccagc acattctgga ctgccttcca
    22021 tccctgatga agtgctgcgg cattaccctg cgcacggatt agccgttgac ccgcgttcgc
    22081 ttgtgaaccg tcgctgaccg cgtcatcaat agttaatccc agcgtagtta acatcttaca
    22141 cgcgacagaa acgccgctga cgcagccgcc ctgacggtga aaaaacgcta tatagccagg
    22201 ctggtgtcca atatttaatg ctcgcgtggt caggtcacct ccctggatat cttccagcag
    22261 taacgcatcg atttgtgcct gagaaaggaa aatcatcaca gtgcctcctg cggtacgcta
    22321 tcccaccaga tgagcgccca gtcgcggctg gcaggggcga gggcacgagc atcttgttgt
    22381 tgataccagt gatataaccg ctcacgctca tcgtcgttaa gcgcgccaag actccagctg
    22441 acgttctgct caaaacgttc ccaggtgctg ttatcctgtt ggcaattttg tccacggata
    22501 aaatcaacgt gggcataaat tcccatctga tacaggacgt tgagcgcaaa gatatagttg
    22561 ggtaattcga tcacttcccg accagccgcc cgctgaatgg ctggagagac gaacgaggtg
    22621 ctgaccagat gagttgtata gacgcgaagc cgtgcctgat tattcagttt gctcattgca
    22681 tggcgcatat cagccaccag agtggagcgg gaagccacgg caatatcgca gcgtggcagg
    22741 tcactccagt cctcttccca ggcgcgctgg atccagtgga cattgtcagc cttcagtgcg
    22801 gcggcgcggc gggcggcaac gttcagcatc ccctgactgt aatcaacgcc ataaaccgta
    22861 gtgagtttgt cagccagcgc cagcgatacg gtgcctggtc cgcagcccat atcgaataag
    22921 gtctgagcgc cttgcaggtc aattttagcg atcagttgtt gcaggtagct gtccgttggc
    22981 gaggcgcaat tttccgccat tttctccgcc cgttgatccc agtgatcggg cgttttttct
    23041 gtgcggtgag ccagtttgag ttgctgcaga taaaggtctg caaaatcaat atcatcaatg
    23101 agcatgattg ttccttagcg gctaattgca gagaggtgat gatgaatttg gtcggcagaa
    23161 acccgataca gggccgccag tttgttcgtt gttaactgtt cggtcggtaa gccttgtgtt
    23221 actcgtccat ctggctctac ttggataatg ctgtcggcaa tggcgttggc atggagcgta
    23281 tggtgagtcg acattaacat tgtcatgccg ttatttttga gctgcgccag cgtgtccaga
    23341 agttggatct gatgaccgaa atcgaggctg gaggcgggct catccagtaa caacaagcgc
    23401 ggttgttgga cgagtgcacg ggcgattaaa gccagctgac gttcaccgcc gctaagcgta
    23461 ttccagcgtc gtgtcgccag atgcaaaatg cccagctttt ccagttgctc cgtggctttc
    23521 aggcgttctt gcttaccggg aacggaaaaa gcgcctatgg tgggggccag gcccatcaac
    23581 accatatcca gcacactgaa agcaaaaata ccgtcgtgcg cctgcggcac ccaggcaata
    23641 gcctgtgcgc gctggcgatg gctgagtgtc tgcacagaga ggctatccag taggatctcg
    23701 ccttttaata acggtataac gccaagaatg gagcgcatga gtgtggtttt gccgcaacca
    23761 ttcgcgccaa gcagacaaca aattgttcct tgcgacaggg tgaaggacac gtcgcgtagc
    23821 actggatggc gggagtaacc cagagacgca tgacgaacgg caagcaaagt cactggcgac
    23881 ctccgcgtaa caacaaaagc aggaaaaacg gtgcgccaac aaaagcggtc agaatgccaa
    23941 gtgggatttc tgttgttcca atgctgcgcg ccagcgtatc ggtaagcagg agcagaatag
    24001 cgccagtaca cattgccata ggcaacagct gctgatggtt atgaccggtc aacaagcggg
    24061 caatgtgcgg aacgaccaga ccgatccaac caattatgcc cgctattgct accgtgctgg
    24121 cggtgattag tgtggcgcag acaatcaatc caaagcgcag acgcgtcacg ttcagtccca
    24181 gcgaacgggc ttcgtcgtcg gaaagcgtga gtaaattcat gcgccagcgg agaaaaaaca
    24241 ggggcaagga gcctatgaga atgatcggtg cagcatagca gagatcgcgt aaggtcacgg
    24301 tggacagtcc ccccagcaac cagaaggtaa ttgacggtaa ctgggtgtaa ggatcggcca
    24361 gcgttttaat tagcgatatc cccgctccgc acaacgttcc taacgcaatg cctaccagca
    24421 ccagggttaa gatcggatcg tggcgggtca cccgacgcgt aatcaaccag acgcccgcga
    24481 ccaccatcag cccgccgcaa aatgcgtaca gctggatgta aacaatgggt aatccccaca
    24541 ggattgccgc gcaggcacca agtccggcac cggcggagac gcctaaaata tccggtgaaa
    24601 ctaagggatt acgcaacatt ccctgatagg tggttccggc tcctgccagc gctgcgccca
    24661 gtaacagcgc agcaagaata cgcggcacgc ggatttgcca gaagacaatt tgttcctgca
    24721 cagactcatt ttcttgacga agaattagcg ccagcagttg ctgcatatcg agatgataag
    24781 ccccacttac ggcggcaaaa gtgatactga tagctgccag cagcaaaata catccgttta
    24841 cgattctcat tagctcgcca ccaatttttg atagtcggca tcactgagtg aacaatgcca
    24901 gaacagttgg gcgtaatgct gcatgtcact tttaaactgg cgattgatgg cgggatccag
    24961 ccaggcgtga agtctgcgta atcccagaag acggttgatt cccggcgggg catcgagcca
    25021 gccaaagggc aagccactta aaaataagat gcgttgttcc gcaacggctt tcacgccctg
    25081 ccagagagga tcacgacgaa taaaatctgc agtaacggcc tcctgaacca gaataatatc
    25141 cggctgccag cgcaggagat tttccatgga aacctgtgtc aggccgtggc gatcggctat
    25201 ttgcgcgacg ttgtgcaaac ctaataattc cgccgcctcg gtatgcaacg aaccctgtaa
    25261 gcccgtttcc agccctcggg gaccgcgcgc agcataaaag cggaggttag cggcgggtga
    25321 ggtggcgaat gcctgagctt caccaacgaa gtggctggcg agattggctt gttctgcggc
    25381 gcggtgctct tcgcctaacg ttttgccaag cgttgttaac tgttctgctg attgcgccag
    25441 ttttccgtta agcaataacc aggggatttg tgtctgttcg ctaacctgcc gtgcctggga
    25501 gatcaaggtt tcatccgtat tgccgcaatc aacgaccaaa tcgggatgta acgccataag
    25561 cccttccagc gatagtgtgc tggcgcgccc ggcgagtcgt cccagcctgg gtaactggcg
    25621 aatgtgctct ggcaatggaa ttaatgcctg acgggcaaaa tcaaaagagg aaaaaccgac
    25681 cattttttct ggcgcgacag ccagcaagag caaatcggcg ggcgcgcctg cgctgacgat
    25741 gcgtgtaatg ttttgggctt cgggaagaga ttgcgaagca aaacgattaa ctgtttgtga
    25801 aaaggacggt agagactgca ggagcagggt agatgcaagt gcctgcgcaa acgtccgtct
    25861 ggttatgggc atggtgaaca tcctgaaaag taaaatcgat atatactttc atatatatcg
    25921 ataaaaatca tgttcaatat caaagtttag ctattgaaaa catgaagatg tgacgact
//
LOCUS       DAZSIO010000042        25890 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZSIO010000042 DAZSIO010000000
VERSION     DAZSIO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.042"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACHX5T_001598"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACHX5T_001598"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFR8466843.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /gene="mipA"
                     /locus_tag="ACHX5T_001599"
     CDS             1794..2540
                     /gene="mipA"
                     /locus_tag="ACHX5T_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR8466844.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="ACHX5T_001600"
     CDS             2630..3391
                     /locus_tag="ACHX5T_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR8466845.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="ACHX5T_001601"
     CDS             complement(3442..4326)
                     /locus_tag="ACHX5T_001601"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR8466846.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACHX5T_001602"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACHX5T_001602"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR8466847.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="ACHX5T_001603"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="ACHX5T_001603"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR8466848.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="ACHX5T_001604"
     CDS             6202..6474
                     /locus_tag="ACHX5T_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR8466849.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="ACHX5T_001605"
     CDS             6844..7920
                     /locus_tag="ACHX5T_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR8466850.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="ACHX5T_001606"
     CDS             7947..9326
                     /locus_tag="ACHX5T_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8466851.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="ACHX5T_001607"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="ACHX5T_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11245
                     /locus_tag="ACHX5T_001608"
     CDS             10409..11245
                     /locus_tag="ACHX5T_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFR8466852.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11250..12197
                     /locus_tag="ACHX5T_001609"
     CDS             11250..12197
                     /locus_tag="ACHX5T_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR8466853.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12207..13187
                     /gene="ydjG"
                     /locus_tag="ACHX5T_001610"
     CDS             12207..13187
                     /gene="ydjG"
                     /locus_tag="ACHX5T_001610"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFR8466854.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13324..14082
                     /locus_tag="ACHX5T_001611"
     CDS             13324..14082
                     /locus_tag="ACHX5T_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR8466855.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14200..15558
                     /locus_tag="ACHX5T_001612"
     CDS             14200..15558
                     /locus_tag="ACHX5T_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8466856.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="ACHX5T_001613"
     CDS             complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="ACHX5T_001613"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR8466857.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="ACHX5T_001614"
     CDS             complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="ACHX5T_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR8466858.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="ACHX5T_001615"
     CDS             complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="ACHX5T_001615"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR8466859.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19503..20054
                     /locus_tag="ACHX5T_001616"
     CDS             19503..20054
                     /locus_tag="ACHX5T_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR8466860.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20171..21214
                     /gene="selD"
                     /locus_tag="ACHX5T_001617"
     CDS             20171..21214
                     /gene="selD"
                     /locus_tag="ACHX5T_001617"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR8466861.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21219..23180
                     /gene="topB"
                     /locus_tag="ACHX5T_001618"
     CDS             21219..23180
                     /gene="topB"
                     /locus_tag="ACHX5T_001618"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR8466862.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23307..24347
                     /locus_tag="ACHX5T_001619"
     CDS             23307..24347
                     /locus_tag="ACHX5T_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR8466863.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24464..25657)
                     /gene="gdhA"
                     /locus_tag="ACHX5T_001620"
     CDS             complement(24464..25657)
                     /gene="gdhA"
                     /locus_tag="ACHX5T_001620"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005094017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR8466864.1"
                     /translation="MERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGG
                     MRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMT
                     ELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEA
                     TGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTV
                     VDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQNE
                     LDVDAAHQLIANGVKAVAEGAYMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEM
                     AQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAML
                     AQGVI"
     gene            25756..>25890
                     /locus_tag="ACHX5T_001621"
     CDS             25756..>25890
                     /locus_tag="ACHX5T_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8466865.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     Q"
BASE COUNT         6582 a         6025 c         6682 g         6601 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcgaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgctgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc caggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa ccgccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatttg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgactgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atgtggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gctccgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 taatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatagggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttgaactgtt
    11221 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    11281 ccgctattgt tgatattcca ttacaaccgg tcagtaaaaa tatctttgat gtagattctt
    11341 atcctcttga aagaatcgca atgagcaccg ggggcgatgc aataaatgaa gcaacaatta
    11401 tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat gcctcagggc
    11461 aatttattct cgaccattgt cgcaaagaga atattgatat tcaaagcctg aaacaggacg
    11521 tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag cggacgtttg
    11581 tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat tttgcgcggt
    11641 ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta ttggatggta
    11701 aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt tgtgccgata
    11761 tgatcaaacc gcggttgaat gaaacgctgg atgatatttg cgaagcattg agttatgtcg
    11821 attatctgtt tccaaatttt gccgaggaaa aattactcac agggaaagag acactggatg
    11881 aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag
    11941 acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc gcaggaataa
    12001 ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt gcggcactgt
    12061 tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg
    12121 ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa caattgctgg
    12181 aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcacg acggatataa
    12241 cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca tggaatggcg
    12301 atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt ggcattaatc
    12361 tgattgatac tgcgctagga tataactttg gcaatagtga agttatcgtc ggtcaggcgt
    12421 taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt gtctgggaac
    12481 gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac ctttccccgg
    12541 aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat tacatcgata
    12601 tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct gaaactgtcg
    12661 cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct gctaacgtcg
    12721 atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt caggcgaaat
    12781 acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt gataatggca
    12841 ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcact atcactcgtg
    12901 attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa aacatgctga
    12961 aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag tgcacaattc
    13021 ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt cttagtgggg
    13081 ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg
    13141 cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata ctcaacacga
    13201 ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag gttattttga
    13261 aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata caggggtgta
    13321 atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa cgataaaaag
    13381 gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat tcgccgcgat
    13441 cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc tgttttaaat
    13501 acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt ttatgaagag
    13561 aagctgctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac catggcagcc
    13621 gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag tgacctgacg
    13681 ttgctaacca actcggcgga agcgattcat gttctggctc agtcagaaat taaagtcgtt
    13741 tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat tactaaagag
    13801 atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct tgatattaac
    13861 agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat gatccgtcag
    13921 gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc ttttgtccag
    13981 ttagctgact ttagtcatat taattatata ataactgata aatcaccggg tgcagagtgg
    14041 atagcatttt gcaaagacaa taatatccag ctggtgtggt aatttaagca cacgttgtca
    14101 atattatttg ttcctgcgac tggaaataaa atgatgataa agatgcggca agtgaatttg
    14161 ccgcaattgc taccttttat agcaaatccg aggcacccaa tggaacaata tgatcaaatt
    14221 ggcgcaagac tggaccgctt gcctttggcc cggtttcatt atcgtatatt tggtataata
    14281 agctttagtc tgttattaac ggggtttttg agttactcag gtaatgtcgt cttagcaaag
    14341 ctggtaagca atggatggtc aaataatttc ctctatgccg cctttacctc ggcattaatg
    14401 tttggttatt tcatcggctc acttactggt gggtttattg gtgactactt tgggcggcgc
    14461 agggcgtttc gcataaatct tctcatcgtc ggtattgctg caacaggggc cgcttttgtc
    14521 cctgatatgt actggctcat tttctttcgc ttcctgatgg gaacaggaat gggggcgctg
    14581 attatggttg gctatgcctc atttacggag tttatccccg cgacggtgcg tggaaaatgg
    14641 tccgcgcggc tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc gataggcgtg
    14701 gtggttatcg ctttttttag ttggcgaata atgtttcttc tgggtggtat tggcatactg
    14761 ttagcctggt ttctctcagg taaatacttt atcgagtcgc cacgatggct ggaagggaaa
    14821 gggcaaatcg ccggtgcaga aagccaactt cgtgaagtag agcagcaaat tgaaagggag
    14881 aagagtattc gtttacccca gcttactttg aaccagagca acagcaaggt taaggtaatc
    14941 aagggtacct tcaggctcct gtttaaaggg gaaatgttac gacgtacatt agttgcgata
    15001 actgttttaa ttgcaatgaa catttcgctt tataccatta ccgtatggat accgaccata
    15061 tttgttaact ccggcattga tgtcgataaa tcaatattaa tgaccgctgt tattatgatt
    15121 ggtgctccgg taggaatatt tattgcggca ttaattattg atcattttcc tcgtcgatta
    15181 tttggctccg ccttacttat tattattgcc gtgttaggct atatctattc aagtcagact
    15241 acagagtggg cgattttaat ctatggtctg gtgatgatct tctttttata catgtatgtt
    15301 tgcttcgcgt cggcggttta tatcccggag ctttggccaa cgcatttacg cctgcgcggt
    15361 tcgggtttcg ttaatgccgt cggacggatc gtcgcagttt tcacgcccta tggcgttgcg
    15421 gcattattaa cacattatgg gtcgatcacg gtgtttatgg tgcttggtgt catgttagtg
    15481 ctctgtgcgc tggttctctc catttttggc atcgaaacgc ggaaggtgtc gctggaagag
    15541 atttctgagg tgaattaaat ctttcgttga tggcatcaat tgccagatgt accactgacg
    15601 tgttttattt ggcctacaat gccagtacag aattgtaggc cagcgtaaaa ttacccctgc
    15661 gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat ctccataaac
    15721 gcgtgtgcac tgtcctgggg ctggatattc acgccacgac agccatcggt aatcacgttt
    15781 accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata gtcagtagcc
    15841 aggcccataa cgatcaattc atcgatttca tgatcgcgca accagtcatc aagcgcggtt
    15901 ttctgccgac gtccgttatc aaaaaaggca ctgtaactgt caactaaagg attttcgcct
    15961 ttatggaaca ccgctgcgat ctctttttga ttcagtaacg gatgtaattg tgcgccttca
    16021 ctgttctgca cacagtgttc aggccagaaa gtttgccgca ggccgccgag ttggcctggc
    16081 gtataaggct ctacgccgtg ctggctggca aagctgccgt gattcgccgg atgccagtcc
    16141 tggctggcga taaccgcttc tccgcgcgac tggcaccagt caataaggcg gttagcaacc
    16201 tccaccgtac tgtcaccttc cggtacggcg agcgcgccac cagcacagaa atcattttgt
    16261 aaatcgacca gtaacagggc gcgatggggc attgcagtct ccttaatcat ccggcgtcag
    16321 ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca gttcctggct
    16381 cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac cgtcaataac
    16441 gccggcatgg gcgagggcgt taccggtggc ataaccactc atattcactt taccggacat
    16501 acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta attcctgcag
    16561 gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca atgctttcac
    16621 cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa tcgtcaccac
    16681 gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt gcggggcggg
    16741 tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa ggtttggaga
    16801 ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc gatacaatcg
    16861 gttattgaaa aagagcgtta cttcgttgat cggataattc ggcgcaacgt acaacgcatt
    16921 cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg accctgtcac
    16981 aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcgg tatacgccat
    17041 cgtgtcggta ccgtgtagaa tgacaaaacc atcatagtcg tcatagtgcg ctttaatatc
    17101 ttcagcaata tgctgccagt cttccggcgt catatcagaa gaatccatca acggcgtata
    17161 ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca gcgccagttg
    17221 gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca tcccgatggt
    17281 cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat gaatgatatc
    17341 gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat gcggataagg
    17401 aaatatgggg cgagacagcg ggaagaggat tcgtcgaggt aaaaaataat gtggccgacg
    17461 atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg caaaacgcat
    17521 aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta acggtagagg
    17581 ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac attgcccgga
    17641 cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca acgtagtatt
    17701 ccagatgcca ctgtttcact tttgcgagct ctgctgcttt ggcaaccgca tcatcgaaat
    17761 ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag acgtggccct
    17821 gggcgatttt atcaatttgc tccggcgtcg aatgacgcgc atcagcaacc agcgtgataa
    17881 agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc ggcggcagtg
    17941 ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta tgaacaccaa
    18001 tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata gaaccggtca
    18061 gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa ccaccagatg
    18121 ccgccatgcc gcccatcgat acaaccacag acttacccgc tgcccgggct gctgccagtt
    18181 cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca cgcaggacaa
    18241 tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg gtatcaccgc
    18301 caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag acgacaccga
    18361 tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg atggcgcgat
    18421 aatttttatc agtcttactc cagccgaact ctttggtcag agctttttcg atttcggcac
    18481 tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta tcgccaccgg
    18541 ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga gcagggatct
    18601 gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc cagcggctgt
    18661 cagcttcgcg ggctgccggt gacatatcat cacgaataaa cggttcaacg gcagatttat
    18721 acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac gatttgtagt
    18781 acagaccgtt ggtagcaaaa ccgtgcagat caaccacgcc ttgcggtgac agccaaattt
    18841 tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca acggcataaa
    18901 ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc atagacggct
    18961 ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg cggtcgtcct
    19021 tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga cgatcggaac
    19081 tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtct ggtttatcga
    19141 cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct ttcgaatcac
    19201 caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata aagaacaggt
    19261 taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa aatccggcaa
    19321 taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg tcacaggcgc
    19381 aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt gcggactgtg
    19441 ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca ggagttaatc
    19501 aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg gctgaacccg
    19561 cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg ccggaccata
    19621 agtccatgca accgtggcat ttttttgtga ttgaagggga agggtgcgag cgtttcagcg
    19681 ccgtactgga acagggggcg attgctgccg gtagtgatga taaagctatc gacaaagccc
    19741 gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc aaagaaaatc
    19801 ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg gcgatgcaaa
    19861 tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta actgaaagtc
    19921 cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt tttctctacc
    19981 tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg acgccgtttg
    20041 taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg cgcaggaatt
    20101 cagacactct cacttatcac ttcacggaat gagggttacc atacccggat tgcattgaca
    20161 ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg gagctggttg
    20221 cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc aggcgaagtt
    20281 tgttgatccg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg tgtacgatct
    20341 gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg ttgataatcc
    20401 tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg cgatgggggg
    20461 caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt ccccggaaat
    20521 tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta ttgcgctggc
    20581 tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa cggggatcgt
    20641 accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac tgttcctgac
    20701 gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgt tgaaaccaga
    20761 acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg cgtcctttgc
    20821 caacatcgaa ggcgtaaaag cgatgaccga cgttacgggc tttggtctgc tgggccactt
    20881 gagcgaaatg tgtcaggggg ctggtgtgca ggcacgcgtc gactatgaag cgatcccgaa
    20941 actccccggt gttgaagagt acattaagtt gggcgcagta cctggcggca ctgaacgtaa
    21001 ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg atctgctgtg
    21061 cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag aaaatgaggt
    21121 taaaactaca gccgccgagt ttggcattga actgacggcg attggcgaac tggtgccagc
    21181 gcgcggcggt cgtgccatgg ttgagatccg ttaatttaat gcggttgttt attgccgaaa
    21241 aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg aaaggcgatg
    21301 gctttatcga gtgcggtaat ggtcaggtgg tgacttggtg tatcggtcac ctgcttgagc
    21361 aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg gatttgccga
    21421 ttgtcccgga aaagtggcaa ttacagccac gaccctccgt gaccaaacaa cttaacgtca
    21481 tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggggacccg gatcgtgaag
    21541 ggcaattgct ggtggatgaa gtgctggact atctgcaact ggcaccggaa aagcgccagc
    21601 aggtgcagcg ttgcttgata aacgacctga acccgcaggc ggttgagcgg gcgatcgacc
    21661 gtcttcgttc caacagtgag tttgtgccat tgtgcgtttc tgcgctggcg cgagcgcgtg
    21721 ccgactggct gtacggcatc aatatgaccc gtgcgtatac cattctcggt cgcaatgccg
    21781 gttatcaggg cgtactttcc gtgggacgcg tgcagacacc cgtgctcggg ctggtggtgc
    21841 gccgcgatga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc aaagcacata
    21901 tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa gcgtgtgaac
    21961 cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat gtggttaacc
    22021 gcattagtgg tcaaccggct attgtcacca gctataacga taaacgggaa tcagaatccg
    22081 cgccgctgcc gttttcgctt tcggcgttgc agattgaagc ggcaaaacgc ttcggtctga
    22141 gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag ctaatcactt
    22201 atccgcgttc cgattgtcgc tatttgccag aagaacattt tgccggacgc cacgcggtga
    22261 tgaatgccat cagcgttcat gcaccggatc tgttgccgca gccagtggta gatccagata
    22321 tacgcaaccg ctgttgggat gacaaaaagg tcgatgcgca ccacgccatc atcccgaccg
    22381 cacggagttc tgcgatcaac ctgacggaga acgaagcgaa ggtctataac ctgattgccc
    22441 gtcaatacct gatgcagttc tgcccggatg cggtgttccg caagtgtgtt atcgaactgg
    22501 acattgccaa aggcaaattt gtcgctaaag cccgttttct tgctgaagca ggctggcgca
    22561 cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg cacgccattg cctgtggtgg
    22621 cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa acccagccgc
    22681 cgcgccattt taccgatgca acactgcttt cggcgatgac cgggatcgcg cgctttgtgc
    22741 aggacaaaga tctgaaaaag gtccttcgtg cgaccgatgg tctggggaca gaagcaaccc
    22801 gtgccgggat tattgaactg ctgttcaagc gtggttttct gaccaaaaaa gggcgctata
    22861 tccactccac cgacgccgga aaagcgttat tccattcgct gccagaaatg gcgacgcgac
    22921 cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag cagtgtcgct
    22981 atcaggactt tatgcagccg ctggtgggga cgctatatca gcttattgat caagccaaac
    23041 gtacgccggt gcggcagttt cgcggcattg tggctccggg cagtggtggc agtgctgata
    23101 agaaaaaggc tgcaccgcgt aaacgtagtg cgaaaaaaag tccgccagca gatgaagccg
    23161 gaagcggggc gatagcgtaa gcgagcgaat ctttcgtgct attcgagtca tattctgaaa
    23221 tatccagcgg atcaagaaag ttcgttggat atttttttgc atggataaaa ttatcgcctc
    23281 taaagtatgt aataacaggg aatgtgatga aaaaagtttt attgcaaaac catcctggga
    23341 gcgagaagta ttcttttaat ggctggggaa tatttaatag taattttgaa cgggtgatta
    23401 aagaaaataa ggccatgctg ctttgtaagt gggggtttta tttaacatgt gttgtcgctg
    23461 taatgtttgt attcgcagcg ataacatcca acggtttgaa tgaaagaggc ctgattaccg
    23521 cgggatgctc ttttctttat ctattaatta tgatggggct tattgttcgg gccggtttta
    23581 aagcaaaaaa agaacaactg cattattatc aggctaaagg tattgagccg ctcagtatcg
    23641 agaagttaca ggcgctacaa ttgatcgcac cttatcgatt ctatcataag caatggtctg
    23701 aaacgctaga gttctggccg cgaaagcctg aacctggcaa agataccttc caatatcatg
    23761 tgcttccttt tgactcgatc gatatcatca gtaaaagacg cgagtcttta gagaatcaat
    23821 ggggtatcga agatagcgaa agttattgtg ccttaatgga gcattttctt tctggcgacc
    23881 atggagccaa tacctttaaa gcaaacatgg aggaagcccc agagcaggtt atcgccttgt
    23941 tgaataaatt tgctgttttt ccctcagact atatctctga ttgcgctaat aatcgctccg
    24001 gtaaatcctc ggcgaagcta atatgggcgg cggaattatc atggatgatc tcgatatcaa
    24061 gcacagcttt tcaaaacggg acaattgaag aagaactggc ctggcattat ataatgcttg
    24121 cttctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga ttatcaaaaa aatagtctaa
    24181 tgggatttct ttactggcat atctgctgct atcgcagaaa gttaacggat gctgaacttg
    24241 aagcgtgtta tcgttacgac aagcagtttt gggagcacta cagtaagaaa tgccgttggc
    24301 ccataagaaa tgttccctgg ggagcatcat ccgttaaata ctcataaacg cctgaaattt
    24361 tgccgggggc gctgcgcttg accggcctac attagagcgt aactgcaatg aattgtgccc
    24421 atctgtaggc ctgataagcg tagcgccatc aggcatttac aacttaaatc acaccctgcg
    24481 ccagcatcgc atcggcaacc ttcacaaaac cggcaatgtt cgcgccctgc acgtagttgg
    24541 tttgctcacc ttcaccacca tgctcaacac aggcatggtg gatatccagc atgatgtgat
    24601 gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg cgcagcgttt tgtgccattt
    24661 ccaggcccga cgttgctaca ccgccagcat tagccgcttt acccggtgca aacagtacgc
    24721 ctgcctgctg gaacagttca gtcgcttcga tggtggtcgg catatatgcc ccttcagcga
    24781 cggctttaac gccattggcg ataagctgat gcgcggcgtc aacatccagt tcgttctggg
    24841 tggcgcaagg cagggcgata tcaaccggta cagaccacgg ctgttggcct tcgagataga
    24901 ccagaccaaa ttctttggcg taatctgccg ctcgaccatc gcggctggct ttgatttcga
    24961 taagacgtgc cagtttctct ttcgtgaatc cgctttcatc aactacagtg ccgctggagt
    25021 ctgacgcagt gatcacacga gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga
    25081 cgttgccgga gccagaaacg gaaacgctca tcccttcaaa acccataccg tggcgtttta
    25141 acattgcttc tgtgaaataa accagaccgt agccggtagc ttccgggcga ataagactac
    25201 cgccaaatga aaggccctta ccggtgaaga cgcaggcggt attgttggag agctttttca
    25261 tcatccccgc cataaagccg acttcacgac caccaacgcc gatatcacct gccggaacgt
    25321 cggtatccgc gcccaggtgg cgatacagtt cagtcatcag cgtctggcaa aaacgcatca
    25381 ctttaccttc gctttttcct ttcggatcga aatcgctgcc gcctttacca ccgcccatcg
    25441 gcagggtagt cagggcattt ttgaaggttt gttcaaagcc gaggaatttg agaatggaaa
    25501 ggttaactga cggatggaag cgcataccgc ctttgtacgg gccgatggca gagctgaact
    25561 gcacacgcca tgcacggttg acctgtatct ggttgcgatc atcaacccat accacgcgaa
    25621 actggatcac gcgctccggt tcaaccagac gctccagtaa tgactagact ggccccctga
    25681 atctccagac aaccagtatc acttatttaa gtgatagtct taatactagt ttttagacta
    25741 gtcattggag aacagatgat tgatgtctta gggccggaga aacgcagacg gcgtaccaca
    25801 caggaaaaga tcgcaattgt tcagcagagc tttgaaccgg ggatgacggt ctccctcgtt
    25861 gcccggcaac atggtgtagc agccagccag
//
LOCUS       DAZSIO010000043        25590 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZSIO010000043 DAZSIO010000000
VERSION     DAZSIO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.043"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..1331
                     /gene="yjeM"
                     /locus_tag="ACHX5T_001622"
                     /pseudo
     CDS             1..1331
                     /gene="yjeM"
                     /locus_tag="ACHX5T_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="ACHX5T_001623"
     CDS             complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="ACHX5T_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR8466866.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFVSGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4815..5783)
                     /gene="asd"
                     /locus_tag="ACHX5T_001624"
     CDS             complement(4815..5783)
                     /gene="asd"
                     /locus_tag="ACHX5T_001624"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR8466867.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="ACHX5T_001625"
     CDS             complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="ACHX5T_001625"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR8466868.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7027..7572
                     /gene="orn"
                     /locus_tag="ACHX5T_001626"
     CDS             7027..7572
                     /gene="orn"
                     /locus_tag="ACHX5T_001626"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR8466869.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7783..7858
                     /locus_tag="ACHX5T_001627"
     tRNA            7783..7858
                     /locus_tag="ACHX5T_001627"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7816..7818,aa:Gly,seq:gcc)
     gene            8016..8091
                     /locus_tag="ACHX5T_001628"
     tRNA            8016..8091
                     /locus_tag="ACHX5T_001628"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8049..8051,aa:Gly,seq:gcc)
     gene            8127..8202
                     /locus_tag="ACHX5T_001629"
     tRNA            8127..8202
                     /locus_tag="ACHX5T_001629"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8160..8162,aa:Gly,seq:gcc)
     gene            8436..8489
                     /locus_tag="ACHX5T_001630"
     CDS             8436..8489
                     /locus_tag="ACHX5T_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466870.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(8472..9611)
                     /gene="queG"
                     /locus_tag="ACHX5T_001631"
     CDS             complement(8472..9611)
                     /gene="queG"
                     /locus_tag="ACHX5T_001631"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR8466871.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            9610..11157
                     /gene="nnr"
                     /locus_tag="ACHX5T_001632"
     CDS             9610..11157
                     /gene="nnr"
                     /locus_tag="ACHX5T_001632"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR8466872.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            11129..11590
                     /gene="tsaE"
                     /locus_tag="ACHX5T_001633"
     CDS             11129..11590
                     /gene="tsaE"
                     /locus_tag="ACHX5T_001633"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR8466873.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            11609..12946
                     /gene="amiB"
                     /locus_tag="ACHX5T_001634"
     CDS             11609..12946
                     /gene="amiB"
                     /locus_tag="ACHX5T_001634"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR8466874.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            12956..14803
                     /gene="mutL"
                     /locus_tag="ACHX5T_001635"
     CDS             12956..14803
                     /gene="mutL"
                     /locus_tag="ACHX5T_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR8466875.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            14796..15746
                     /gene="miaA"
                     /locus_tag="ACHX5T_001636"
     CDS             14796..15746
                     /gene="miaA"
                     /locus_tag="ACHX5T_001636"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR8466876.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            15832..16140
                     /gene="hfq"
                     /locus_tag="ACHX5T_001637"
     CDS             15832..16140
                     /gene="hfq"
                     /locus_tag="ACHX5T_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR8466877.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            16216..17496
                     /gene="hflX"
                     /locus_tag="ACHX5T_001638"
     CDS             16216..17496
                     /gene="hflX"
                     /locus_tag="ACHX5T_001638"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR8466878.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            17582..18841
                     /gene="hflK"
                     /locus_tag="ACHX5T_001639"
     CDS             17582..18841
                     /gene="hflK"
                     /locus_tag="ACHX5T_001639"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR8466879.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            18844..19848
                     /gene="hflC"
                     /locus_tag="ACHX5T_001640"
     CDS             18844..19848
                     /gene="hflC"
                     /locus_tag="ACHX5T_001640"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR8466880.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            19930..20127
                     /locus_tag="ACHX5T_001641"
     CDS             19930..20127
                     /locus_tag="ACHX5T_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR8466881.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            20231..21529
                     /gene="purA"
                     /locus_tag="ACHX5T_001642"
     CDS             20231..21529
                     /gene="purA"
                     /locus_tag="ACHX5T_001642"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR8466882.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            21734..22159
                     /gene="nsrR"
                     /locus_tag="ACHX5T_001643"
     CDS             21734..22159
                     /gene="nsrR"
                     /locus_tag="ACHX5T_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR8466883.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            22198..24639
                     /gene="rnr"
                     /locus_tag="ACHX5T_001644"
     CDS             22198..24639
                     /gene="rnr"
                     /locus_tag="ACHX5T_001644"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR8466884.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            24819..25550
                     /gene="rlmB"
                     /locus_tag="ACHX5T_001645"
     CDS             24819..25550
                     /gene="rlmB"
                     /locus_tag="ACHX5T_001645"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR8466885.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
BASE COUNT         5992 a         6486 c         7185 g         5927 t
ORIGIN      
        1 ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat ctattcctgg
       61 atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg gttttcctct
      121 tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc aacattcctc
      181 tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac gcaggtggtt
      241 ggtctgctgg cagtagcatg gatgattctg gtcacagtcg ttgcttcaaa ggggattaat
      301 aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa tttagtattg
      361 ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca ggatattaat
      421 ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc gtttgtggta
      481 tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga taaaacggaa
      541 aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat ttcaatcggt
      601 tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt attaagtaat
      661 ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg ggtgatgctg
      721 ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg gtttgcgcgt
      781 attaccggac tttcgatgtt cctcgcttat accggtgcgt ttgctattca ccgctgaaag
      841 ccatcatcca ggggacgccg aaagcgttgt ggccggaacc gatgacgcgc ctgaatgcga
      901 tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt ggttactgtc ttcattctgc
      961 tggtttcgtt tggtggcggt accgcatcgg cgttctttaa caagctgacg ctgatggcga
     1021 acgtgtctat gacgcttcct tacctgttcc tcgcgctggc tttcccgttt tttaaagcac
     1081 gtcaggatct cgacagacca tttgtgattt tcaaaacgcg tatgtcggca atgattgcga
     1141 cggtggttgt cgttctggtg gtgacatttg cgaacgtctt caccattatt cagcctgtgg
     1201 ttgaagccgg agactgggac agcacattgt ggatgattgg cggccctgtc ttcttctcgc
     1261 tgttagcgat ggcgatttac cagaactatt gcagtcgcat ggcgaataaa cctgagttag
     1321 ctctcgactg atcatatgaa atcctgcaac tcatcgtctc tccggcgatg agttgtcagg
     1381 aagtaatgcg tatggatgat actctccagg gccggtgata actgaagatg atgttaagct
     1441 gctaaatgag ccacgttgac atcatttggc agcttcatgc attacaaact tcccgcctga
     1501 cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt taccgttgag gctttccaga
     1561 cgcatctgga agggcgggaa tggcatatcg ataccgtggg cgtggaagcc tgccagaatc
     1621 agctggtgga tctcatggcg tagcggcata cggtgaccca tctcagcggc gtaaatacgc
     1681 agctcgaaaa tctgaatccc ctgttgcaga tccaccagga agacttccgg tgccgggttg
     1741 tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga tttccgtcac ttcttcactg
     1801 ttggcatcgg caggggccgg aatcgtcaac actacgcgcg tgaccgagtc agagagcgac
     1861 cagttgataa actgctcggt aataaacgcc ttgttcggca cgattatctc tttacggtcc
     1921 cagtcgctga tggtggtggc gcgggtgtta atttttgtca cgctaccagt aagatcgcgg
     1981 atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca ggatgatcag gccagagaca
     2041 aagttggcga aaatttcctg caaaccaaaa ccgagaccaa caccgagcgc ggcaaccagc
     2101 cactgcaatt tcgaccactc aataccaatc attgagaagc cgaccagccc gccaatcagc
     2161 atcagcagat attttgtgat ggtggtgatg gcgtaacccg tacccggcgt taaatccagg
     2221 tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc gcaccagctg cgtggtgatg
     2281 ataaatacca gaatggcaat cagcaccgca ccgagggtaa ttggctccag actttctacg
     2341 ccctgtaccg tggaggtgac atcccacagc gaaatatttt cgaggaagcc gaaagcggaa
     2401 tggatttctg accacagcac aatgaccgaa agcagggcga tcaacatcaa aattgagcgc
     2461 accagccgca aggattgcgc actgatggca tcgagatcga cttcgctttc atcgacttca
     2521 attgctcctt ccgggctact gtgatgatgc gcttcctctt cgccacgcgc acgttgcgct
     2581 aacatctctg cccggcgatg cttcgcccgg tcaaacgcca gcctgcgacg ctggatcagc
     2641 atccagcggc ggataacgtg ataaaccacc agtagcagga accagatggc aaccgaggtt
     2701 tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac ccaccgccga cgccagaatg
     2761 gcaaccagtg gtgcgccaat catcatgttc cacagcatat ggttggtaat gttgtcgccg
     2821 ctgccttctt tgttgagata caacggaata ccggcttttt tcaggcttag ggtgaccacc
     2881 gccagcgcac cgcaaatgag gataaagcaa agccgtccca gcgaaccgga aaattcgcgg
     2941 tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc cgatgctcat caggtagtag
     3001 cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat gagcgataaa caagccgttc
     3061 gggcgggcaa aggtggcgca aatcatcact acccacagca gcggcacggt ggccgttaca
     3121 ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct cgcgcaagcc gtaacccagc
     3181 gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg accagaaaag agtgcgcaac
     3241 gtcagccaga agtgatcctg agtcactttg ccgactttcg ccgccgaacg ttcaagaaaa
     3301 cgggtgaaat agcggcgcga gtaaatactg caaccgacca gaatcaacgc gccaaacagc
     3361 ggcaaaattg tctctttgct ggtcagcatc atcacactgg ctttgcccaa ctgactgaag
     3421 gtgtccagcg aaatgagacg acgcagatcc tgggcgattt ccagcggcca ggcgatggtc
     3481 atcgggcgca cgtcagaggc ccagaacaga tagcggtgcg ttgcttcgtt cacctctttc
     3541 agcgcatcct ccagttgcct gttggagact ttcagcttgg tcagttccaa tagtagcgtg
     3601 tcgccaccct gcaacaatga gttcagcaac tcacgctgag tgcgcagttg tgcttccaga
     3661 atacggtttt gctcggcggt cagcggctga ccgtcggcct gatggatttg ccgtagcagc
     3721 ggctgtttat taagtaaatc ctcataacgt aaccgttgca cacgcaactg cgccatttcg
     3781 gtatcaagct gttgtggttt tggcatttct ggtagccgtg ccacctgcgc ccgcagcgct
     3841 tcgccgagca ggttggacga tcccagccat tgcgactgtt cacgcagcgt attcaacgcc
     3901 tgccggacct gtaacgtctg gctggcagcc tgacgctgtt gcgaggcaac gagctccatc
     3961 cgctgcgcct gttgattcag cgctgccgat agttcgcggt taattttgaa ttgcgcgacg
     4021 atatctttcg gcaaatcggc actgttttct gccagtaatt cggtactttc cagcgcccgc
     4081 tccgcctcaa gctgacgttg gctattcaac tgattacgta aggcctgcaa atacgcatcc
     4141 agttgctggc tctctttttc cgccagctct gagcgtaagc gcgctaattc ctggcggtta
     4201 ttggcagaca gctgcgccag ctccagttca tcaacgagcg ccttaagacg tgcagagtca
     4261 gactgcaacg cgaaattttg tgcctgattg agcggagtat tgccggtaag cgttcccagg
     4321 cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt gctgttgcgg cagttgattc
     4381 agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct gggcctgacg gcttttatcc
     4441 agcaactggc tgctgacctg gagaatttcc tgattcagcg cgtcggtaga cattcccggc
     4501 gacacgctgc gcggctcgtc acgaatgttg tttaattgtg cgcgcagagt agcggagagt
     4561 ttcggataat tatcgataac ttgctgatat tgtttgatgc gctcaaggga accttttcgt
     4621 tcctcaagcg catttaaggc agactggagc gcctctacga cttccggctg tgcgggtttc
     4681 gccgcttttg cctgctccag ttcctgagtg atttgtttgc tatcgggggc cgtcgcggcg
     4741 tacgcccccc aactgaggca ccaggccatc agaaaagtga taatcaggcg cacgtcagcg
     4801 tttcctttga tggattagac ctggtctttt ttatcgtcaa ccaatgggct ggcgtcgtgt
     4861 tctgcttcga tctcttcagc aggaagtggg gcgggttcag cgtctggcgt aacaaaggtt
     4921 tcggtagata ctgccagcgg ctggccaatt ttcgtgacag acaggctttc cagttgctca
     4981 accagattca ctttacccgg tgcaaacagg ttgataacgg tggaaccgag tttaaagcga
     5041 cccatttcct ggcctttcag cagtgccaca gaaccgtcgt tttccccggc aggccaggtc
     5101 cagcgcttga tgataccttc gcgcggcggc gtaatggtgc ccgcccagac cgtctcaatg
     5161 ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg ggccaaattc ggtatcgaaa
     5221 aggcaaatca cgcgttcgtt acgggcaaac agatttggca cgttctgagc ggtgagatgg
     5281 ttaacggaaa agagatcgcc cggcacgtag atcatctcac gcagaatacc gttgcacggc
     5341 atgtgtacgc gatggtagtc acgcggggag aggtaagtgg tcacaaacgt accgttgcgg
     5401 aacaggtccg ccatcagata gttgcctgcc agcagggctt cgaggctgta gttgtggcct
     5461 ttggcttgca ggattttatc ttcttcgatt ttacccaact ggctgataac gccatcggca
     5521 ggcatgacca gtacattcgg atcggtatcg attgggcgta cttcgtcacg cagcggacgg
     5581 acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg gcttttgcgc ctctttcatg
     5641 tcgaccttgt agtatttaac gaacagatcg ataaccagtt ttgtcagcca tcctgcccgc
     5701 ttgcttgcgc cccaacccgc caggcgagta agccatagtt tcggcagaat gtactgtagc
     5761 gaaagtttaa atgaatttaa caaggtagcc tccaggccat tgttttgtcg ttcctgatcc
     5821 ggccaacatg ccggatcctg aaaaaaaggg gacgattcta acgacggtta gcttaattgt
     5881 cagtcatccg tatcagaaaa gtttttacgc gtttttacct gcgccatgct ttccagaata
     5941 cggtgatagt tttcgaaacg ggtttccgcg attttccctt cctcaaccgc ttcccggata
     6001 gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt tacacagacc taaatagtca
     6061 tggaattcga caaagccctg agtgatttgt tccggctcca ggtgccagag gccgaactca
     6121 cgcacaccag gggaatcaat cacatcaccg ccgtgcggga agtgatacag ccgagcggcg
     6181 gtcgtggtgt gctgaccgag ccccgagttg tcagagacat cgttggtcag gatctctttt
     6241 tgcagcccca gcaacgcgtt cagcaggctg gatttaccga cgccagactg cccggcaaaa
     6301 atgctgatgc gcccggtcaa cgcctcttcc agcggtttca gcccatcctg agtatggctg
     6361 gaaaccatca atacgcgata accgatgttg cggtagatat ccatctgctc gttgacgaac
     6421 gccatgcctt cgtcgtccag caggtctatc ttgttgagca cgataatcgg ctcaatctgc
     6481 aaggtttcgc aggccaccag gtaacggtcg ataatattga gcgacagctc cggcaaaatg
     6541 gcggagacaa tgacaatctg gtcgatgttg gcggctatgg gtttcacgcc gtcgtagaaa
     6601 tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt ccacgatccc tttgacattt
     6661 acgccttccg ccgccggttt acccggacgc cagactacgc ggtcgccggt taccagcgaa
     6721 cggatggtac ggcgaatatt gcagcggtga acgtcgccat cggcggattc cacatcagcg
     6781 tgcataccaa agcggctgat gacgatacct tcatcaggct cgccaaacag attgtcgtcg
     6841 tagtcgggct tctccttaga cgttttaaga cgacgctggt gattggcgtt cacgcggcgc
     6901 tgctggcctt tggagagttt atttttactc aatcgtactg actcctggtc gcccctgatg
     6961 ggcaaaacat ctatgataca cgcaattgtg gatcaatata gtcactgtga atgggtggaa
     7021 aatagcatga gtgccaatga aaacaacctg atttggatcg atcttgagat gaccggtctg
     7081 gatcccgagc gcgatcgcat tattgagatt gccacgctgg tgaccgatgc caatctgaat
     7141 attctggcag aagggccgac cattgcagta catcagtccg atgaacagct ggcgctgatg
     7201 gatgactgga acgtgcgcac ccataccgcc agcgggctgg tagagcgcgt gaaagcgagc
     7261 acgatgggcg atcgggaagc tgaactggca acgctcgaat tcttaaaaca gtgggtgcct
     7321 gcgggaaaat cgccgatttg cggtaacagc atcggacagg accgtcgttt cctgtttaaa
     7381 tacatgccgg agctggaagt ctacttccac taccgttatc tcgatgtcag caccctgaaa
     7441 gagctggcgc gccgctggaa gccggaaatt ctggatggtt ttaccaagca ggggacgcat
     7501 caggcgatgg atgatatccg tgaatcggtg gcggagctgg cttactaccg cgagcatttt
     7561 atcaagctgt aaaatttttc ctggtcacgt aagcgccgga atccacttgc ggcggcgtga
     7621 ttttgacgct aaatcgaacg tcttgctgat aatttcagca cttgagataa aaacgcaaaa
     7681 aaaacttttt tggggggttg cagagggaaa gatttctcgt ataatgcgcc tcccgtaacg
     7741 acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt acgcgggaat agctcagttg
     7801 gtagagcacg accttgccaa ggtcggggtc gcgagttcga gtctcgtttc ccgctccaaa
     7861 atttgaaaag tgctgcaaag cacagaccac ccaatcgcag tatttcgaat tgcgacaagg
     7921 cggcaactga acgaatcctt gggaacttac aaaagtatgt gactaaggtg agtgaaggca
     7981 gccaaggcag tagcggttcg aaagacgaag attaagcggg aatagctcag ttggtagagc
     8041 acgaccttgc caaggtcggg gtcgcgagtt cgagtctcgt ttcccgctcc aaaatttaaa
     8101 agtgctgtaa ggcatagacc acccaagcgg gaatagctca gttggtagag cacgaccttg
     8161 ccaaggtcgg ggtcgcgagt tcgagtctcg tttcccgctc caaattcttc tctcaataaa
     8221 atatctacag cgacgcgatg cgttattgct ggtttttgtt gtctctgaca aactcttgta
     8281 aacagagtta tccacagcct caggctgtaa tcttaatttc aaagaaactt cgcacggtga
     8341 atggtatttt tttaacctat tgataaataa gttaaaaatc aagattccgt tttgtcgagt
     8401 cgatcacttg acgattttat tcgtcttgaa ttgcaatgcg tttttatttt tattcacaag
     8461 ctgtggatga atcaggcgtc acgcggtaac cctttttcaa tcacccgaac cagacgctgt
     8521 tttttcggca attgcacttc gactatgcac gcatttcgtc tctctatttg ctgcgcaatc
     8581 gcccacgcta tgtgctcatc gagaagtggg tgctcacctt tacgactttc cagcgctgtc
     8641 aaaatcgttt catcccaagg ggcattgcct aatgccacgg cgatattacg cagccagcgc
     8701 aggtgaccta tacggcgaat cgccgatcct tccgtgactt ttaaaaactt ctcttcgctc
     8761 caggcaaata actcaatgag ttccggtgcg tgtagcggct tacgcgggct gaaatcgtct
     8821 tctgtagtga gttgcgaata gcgattccac gggcagataa gctggcagtc atcgcaaccg
     8881 taaatacggt ttcccattaa cggtcgcaac tcttccggga gcgccccttc aagttcgatg
     8941 gtgagataag agatacagcg gcgagcatcg acggtatatg gctcgacgat ggcaccggtc
     9001 gggcaaatcg tcatacaggc cacgcatttg ccgcatcctt cctcgactgg ttgatccacg
     9061 ggcagcggaa tatcgaccag caattcgcct aaaaagaaga acgaaccggc ctcgcgattg
     9121 aggataagtg agtgcttacc tgtccagccg agcccagctt tttcagctaa cgggcgctcg
     9181 agaataggcg cagaatcgac aaacggtcta aaattcagcg aaacacaatg ttgctgaatc
     9241 atctcgccca gctttttgag tcggttgcgc agaagtttgt gatagtcacg gcccagcgca
     9301 taacggctaa catagccgag tttggggttt ttcagcgtgc tggcaaatgc ggcgttagca
     9361 ggaaggtaat tcatccgcac gctgattaca cgcagcgtac cgggtaataa ctcatggggg
     9421 cgagcgcgca gcataccgtg acgtgccatc cagtccattt cgccgtggta ttgtttgtcc
     9481 agccatgctt gcagtttggg ctcggactcg ctgagatcgg tatcggtaat acctacctgc
     9541 tgaaagccca gttccagccc ccactgttta attttttgcg ctaactgatt gagatcgagg
     9601 ggctctgaca tgacggacca tacaatgaag aaaaaccccg taagtatacc acacaccgtc
     9661 tggtacgccg acgatatccg ccgcggagaa cgcgaggcgg cagatgtgct ggggctcaca
     9721 ctctatgagc tgatgcttcg cgctggcgag gccgcattcc aggtgtgtcg ttcggcgtat
     9781 cctgacgccc gccactggct ggtgctgtgc ggtcatggta ataacggcgg cgatggctac
     9841 gtggtcgcgc gactggccaa agcggtcggc attgaggtca cgttgttggc ccaggagagc
     9901 gacaaaccgt tgccggaaga ggccgcgctg gcacgcgaag catggttaaa cgcgggtggc
     9961 gagatccatg cttcgaatat tgtctggccc gaatcggtag atctgattgt tgatgcgctg
    10021 ctcggtaccg gcttgcagca agcgccccgc gaatccatta gccagttaat cgaccacgct
    10081 aattcccatc ctgcgccgat tgtggcggtt gatatccctt ccggcctgct ggctgaaacc
    10141 ggcgctacgc caggcgcagt gatcaacgcc gatcacacca tcacttttat tgcgctgaaa
    10201 ctaggcttgc tcactggaaa agcgcgggat gttacaggac aactgcattt tgactcactg
    10261 gggctggata gctggctggc aggtcaggag acgaaaattc agcggttttc ggcagaacaa
    10321 ctttctcact ggctgaaacc gcgtcgcccg acttcgcata aaggcgatca cgggcggctg
    10381 gtgattatcg gtggcgatca cggcacggcg ggggctattc gtatgacggg ggaagcggcg
    10441 ctacgtgctg gtgctggttt agtccgagta ctgacccgca gtgagaacat tgcgccgctg
    10501 ctgactgcac gaccagaatt gatggtgcat gaactgacta tggactctct taccgaaagc
    10561 ctggaatggg ccgatgtggt ggtgattggt cccggtctgg gccagcaaga gtgggggaaa
    10621 aaagccctgc aaaaagttga gaattttcgc aaaccgatgt tgtgggatgc cgatgcattg
    10681 aacctgctgg caatcaatcc cgataagcgt cacaatcgcg tgatcacgcc gcatcctagc
    10741 gaggccgcac ggttgttagg ctgttccgtc gctgaaattg aaagtgaccg cttacattgc
    10801 gccaaacgtc tggtacaacg ttatggcggc gtagcggtgc tgaaaggtgc cggaaccgtg
    10861 gtcgccgccc atcctgacgc tttaggcatt attgatgccg gaaatgcagg catggcgagc
    10921 ggcggcatgg gcgatgtgct ctctggtatt attggcgcat tgcttgggca aaaactgtcg
    10981 ccgtatgatg cagcctgtgc aggctgtgtc gcgcacggtg cggcagctga cgtactggcg
    11041 gcgcgttttg gaacgcgcgg gatgctggca accgatctct tttccacgct acagcgtatt
    11101 gttaacccgg aagtgactga taaaaaccat gatgaatcga gtaattccgc tccctgatga
    11161 gcaggcaaca ttagacctgg gcgagcgggt agcgaaagcc tgcgatggcg caaccgtaat
    11221 ctatctgtat ggcgatttag gcgcaggtaa aaccaccttt agccggggct ttttacaggc
    11281 tctgggtcat cagggtaatg tcaaaagccc cacttatacg ctggtcgaac cctatacgct
    11341 cgacaactta atggtctatc actttgattt gtaccgcctt gccgatcccg aggagctgga
    11401 gtttatgggg atccgcgatt attttgccaa cgatgccatc tgcctggtgg agtggccaca
    11461 acaaggtaca ggtgttcttc ctgacccgga tgtcgaaata cacattgatt atcaggcaca
    11521 aggccgtgag gcgcgcgtga gtgcggtttc ctctgcgggt gaattgttgc tggcgcgttt
    11581 agccggttaa cctttgaaag gtggcgggat gatgtatcgc atcagaaatt ggttggtagc
    11641 gacgctgctg ctgctgtgca cgccggtggg tgccgcgacg ctctctgata ttcaggtttc
    11701 taacggtaat caacaggcgc ggataacgtt gagttttatt ggcgatcctg attatgcgtt
    11761 tagccatcaa agcaaacgca ccgtggcgct cgatatcaaa caaacgggca tgattcaggg
    11821 actgccgttg ttgttcagcg gcaataatct ggtgaaggcg attcgctctg gaacgcctaa
    11881 agatgcacaa acgctacggc tggtggtcga tcttaccgaa aacggtaaaa ccgaagcggt
    11941 gaagcggcag aatggcagca attacactgt cgtctttacg attaacgccg atgtgccgcc
    12001 accgcctcct ccgccgcccg tggttgcgaa acgcgttgaa acgcctgcgg ttgtcgcacc
    12061 gcgcgtcagc gaaccggcgc gcaatccgtt taaaacggaa agtaaccgca ctacgggtgt
    12121 tatcagcagt aatacggtaa cgcgtccggc agcgcgcgcg acggctaaca ctggcgataa
    12181 aattatcatc gctattgatg ccggacacgg cggtcaggac cctggcgcta tcggccccgg
    12241 tggtacgcgg gagaaaaatg tcaccatcgc catcgcgcgt aaattgcgta ctttgctcaa
    12301 tgacgatccg atgtttaaag gcgttttaac ccgtgacggg gattacttta tctcggtgat
    12361 ggggcgcagt gatgtggcac ataagcaaaa cgccaatttc ctcgtgtcga ttcacgctga
    12421 tgccgcaccg aaccgtagtg cgactggcgc ttccgtatgg gtgctctcta accgtcgcgc
    12481 caacagtgaa atggccagct ggctggagca gcacgagaaa ctgtcggagc tgctgggtgg
    12541 ggcgggtgat gtgctggcga acagtcagtc tgacccctat ttaagccagg cggtgctgga
    12601 tttacagttc ggtcattccc agcgggtagg gtatgatgta gcgaccagta tgatcagtca
    12661 gttgcaacgc attggcgaaa tacataaacg tcgaccagaa cacgccagcc ttggcgttct
    12721 gcgctcgccg gatatcccat cagtactggt cgaaaccggt tttatcagca acaacagcga
    12781 agaacgtttg ctggcgagcg acgattacca acaacagctg gcagaagcca tttacaaagg
    12841 cctgcgcaat tacttccttg cgcatccgat gcaatctgcg ccgcagggtg caacggcaca
    12901 aactgccagt acggtgacga cgccagatcg cacgctgcca aactaaggac gattgatgcc
    12961 aattcaggtc ttaccgccac aactggcgaa ccagattgcc gcaggtgagg tggtcgagcg
    13021 acctgcgtcg gtagtcaaag aactagtgga aaacagcctc gatgcaggtg cgacgcgtat
    13081 cgatattgat atcgaacgcg gtggggcgaa acttatccgc attcgtgata acggctgcgg
    13141 tatcaaaaaa gatgagctgg cgctggcgct ggctcgtcat gccaccagta aaatcgcctc
    13201 tctggacgat ctcgaagcca ttatcagcct gggctttcgc ggtgaggcgc tggcgagtat
    13261 cagttcggtt tcccgcctga cgctcacttc acgcaccgca gaacagcagg aagcctggca
    13321 ggcctatgcc gaaggacgcg acatggacgt gacggttaaa ccggcagcgc atccggtggg
    13381 aacgacgctg gaggtgctgg atctgttcta caacaccccg gcgcggcgca aattcctgcg
    13441 caccgagaaa accgaattta gccatattga tgaaatcatc cgccgcattg cgctggcgcg
    13501 tttcgacgtc acgatcaacc tgtcgcataa cggtaaaatt gtgcgtcagt accgcgcagt
    13561 gccggaaggc gggcaaaaag aacggcgctt aggcgcgatt tgcggcaccg cttttctcga
    13621 acaagcgctg gcgattgaat ggcaacacgg cgatctcacc ctgcgcggct gggtggccga
    13681 tccaaatcac actacgcccg cactggcgga aattcagtat tgctacgtga atggtcgcat
    13741 gatgcgcgat cgcctgatca atcatgcgat ccgccaggcc tgcgaagaca aactgggggc
    13801 cgatcagcaa ccggcatttg tgttgtatct ggagatcgac ccgcatcagg tggacgtcaa
    13861 cgtacacccc gccaaacacg aagtgcgttt ccatcagtcg cgtctggtgc atgattttat
    13921 ctatcagggc gtgctgagcg tgctacaaca gcaactggaa acgccgctac cgctggacga
    13981 tgaaccacaa cccgcaccgc gtgccatccc ggaaaaccgc gtggcggcgg ggcgcaatca
    14041 ctttgcagaa ccggcagctc gtgagccggt agctccgcgc tactctcctg cgcccgcctc
    14101 aggcagtcgt ccggctgccc cctggccgaa tgcgcagcca ggctaccaga aacagcaagg
    14161 tgaagtgtat cgccagcttt tgcaaacgcc cgcgccgatg caaaaaccaa aagcgccgga
    14221 accgcaggaa cctgcacttg cggcgaacag tcagagtttt ggtcgggtac tgactatcgt
    14281 ccattccgac tgtgcgttgc tggagcgcga tggcaacatt tcacttttat ccttgccagt
    14341 ggcagaacgt tggctgcgtc aggcacaatt gacgccgggt gaagcgcccg tttgcgccca
    14401 gccgctgctg attccgttgc ggctaaaagt ttctggcgaa gaaaaatcgg cattagaaaa
    14461 agcgcagtct gccctagcgg aattgggtat tgatttccag tcagatgcac agcatgtgac
    14521 catcagggcc gtgcctttac ccttacgcca acaaaattta caaatcttga ttcctgaact
    14581 gataggctac ctggcgaagc agtccgtatt cgaacctggc aatattgcgc agtggattgc
    14641 acgaaatctg atgagcgaac atgcgcagtg gtcaatggca caggccataa ccctgctggc
    14701 ggacgtggaa cggttatgtc cgcaacttgt gaaaacgccg ccgggtggtc tgttacaatc
    14761 tgttgattta catccggcga taaaagccct gaaagatgag tgatatcagt aaggcgagcc
    14821 tgcctaaggc gatttttttg atggggccga cggcctccgg taaaacggcg ttagccattg
    14881 agctgcgtaa aattttacca gtagagttga taagcgttga ttctgtcctt atttacaaag
    14941 ggatggatat cgggacggcg aagccgaacg ctgaagagtt actcgccgcg ccgcaccgat
    15001 tgctggatat tcgcgatccg tcgcaggctt actcggctgc tgattttcgc cgcgatgcgc
    15061 tggcggaaat ggccgatatc accgcggcgg ggcggatccc actgttagtg ggcggtacga
    15121 tgttgtattt caaggcattg ctggaagggt tgtcgccgct accgtcggca gacccggaag
    15181 tgcgggccag aattgagcaa caggcggcag agcaaggttg ggagtcattg catcgtcaac
    15241 ttcaggaggt agatccggtt gcggcagcaa ggattcatcc aaatgatcca caaaggcttt
    15301 cccgggcact ggaagttttt ttcatttcgg gtaaaacttt aacggaactg acgcaaacgt
    15361 caggagacgc tctaccgtat caggtgcatc agttcgccat cgccccggcg agccgtgaac
    15421 tgctccatca acgcattgag cagcgttttc atcagatgtt ggcttcaggt tttgaagcag
    15481 aagtccgggc gctttttgcc cgaggagatt tgcatacgga cttgccttcc attcgttgcg
    15541 tgggttatcg ccagatgtgg tcttaccttg aaggcgaaat ctcatacgat gaaatggttt
    15601 atcgaggtgt ttgcgccacg agacagttgg cgaagcggca gataacctgg ctgcgtggtt
    15661 gggaaggggt tcactggctt gacagtgaaa aaccagaaca ggcgcgtgac gaagtattac
    15721 aggttgttgg tgctatcgca ggctgaatgt gtacaattga gacgtatcgt gcgcaatttt
    15781 ttcagaatcg aaaggttcaa agtacaaata agcatataag gaaaagagag aatggctaag
    15841 gggcaatctt tacaagatcc gttcctgaac gcactgcgtc gggaacgtgt tccagtttct
    15901 atttatttgg tgaatggtat taagctgcaa gggcaaatcg agtcttttga tcagttcgtg
    15961 atcctgttga aaaacacggt cagccagatg gtttacaagc acgcgatttc tactgttgtc
    16021 ccatctcgcc cggtttctca tcacagtaac aacgccggtg gcggtaccag cagtaactac
    16081 catcatggta gcagcgcgca gaatacttcc gcgcaacagg acagcgaaga aaccgaataa
    16141 ggtttcgggc tgttttttta cacggggagc cagcgatcct gcgttccccg ctgatctatt
    16201 tagagggtta tacgcttgtt tgaccgttat gatgctggtg agcaggcggt actggtacac
    16261 atctatttta cgcaagacaa agatatggaa gacctccagg agtttgaatc tctggtctct
    16321 tccgccggtg tcgaagcatt gcaggtgatt accggtagcc gtaaagcgcc gcacccaaag
    16381 tattttgtag gtgaaggtaa agcagttgaa attgcggaag ctgtcaaagc gatgggtgct
    16441 tcggtcgttc tttttgacca tgccctgagc ccggcgcaag agcgtaacct ggagcgtttg
    16501 tgcgagtgtc gtgttatcga ccgcaccggc cttattttag atattttcgc ccaacgtgcg
    16561 cgtacccatg agggtaagtt gcaggttgag ctggcgcagc tgcgccatct ggctacgcgc
    16621 ctggtgcgtg gctggaccca ccttgaaaga cagaaaggcg ggataggttt gcgtggtccg
    16681 ggtgaaaccc agctcgaaac cgaccgtcgt ttgttgcgta atcgcatcgt gcagatacag
    16741 tcgcgcctgg aaagagttga aaagcagcgt gagcaggggc ggcaatcgcg tatcaaagcc
    16801 gacgttccta ctgtttcgct ggtgggatat accaacgccg gtaaatctac ccttttcaat
    16861 cgcatcaccg aagcgcgggt ctacgcggca gaccagttgt ttgccaccct cgacccgacg
    16921 ttgcggcgta ttgacgttgc agatgtcggt gaaaccgtac ttgcagatac cgtagggttt
    16981 attcgccacc tgccgcacga tctggtggcg gcatttaaag ccacgttaca agagacgcgg
    17041 caagccacat tactgctgca cgtcattgat gcggcggatg tgcgtgtaca agaaaacatc
    17101 gaagcggtga atacggttct tgaagagatc gacgctcacg agatcccaac cctgctggtg
    17161 atgaacaaga tcgatatgct ggaagatttc gaaccgcgta ttgatcggga cgaagagaac
    17221 aaaccgatcc gtgtctggct ttccgcacag accggagcgg ggataccaca gctttttcag
    17281 gctttgacgg agcggctttc cggcgaggtg gcgcagcata cattgcgtct gccaccgcag
    17341 gaagggcgtc tgaggagtcg tttttatcag cttcaggcaa tagaaaaaga gtggatggag
    17401 gaggacggca gcgtaagtct gcaagttcgt atgccgatcg ttgactggcg tcgcctctgt
    17461 aaacaagaac cggcgttgat cgattacctg atctaacggc gtagcgtctg aagcgtggag
    17521 tcatatcctc tggcgtcgaa agacaacagg gatcaccgca taacaaatat ggagcacaaa
    17581 catggcgtgg aatcagcccg gtaataacgg acaagaccgc gacccgtggg gaagcagcaa
    17641 acctggcggc aactctgagg gaaatggaaa caaaggcggt cgcgatcaag ggccacctga
    17701 tttagatgat atcttccgca aactgagcaa aaagctcggt ggtctgggcg gcggtaaagg
    17761 caccggatct ggcggtggca gttcatcgca aggcccgcgc ccgcagcttg gcggtcgtgt
    17821 cgttaccatc gcagcggcag cgattgtcat tatctgggcg gccagtggtt tctataccat
    17881 taaagaagcc gaacgcggcg tggtaacacg ctttggtaaa ttcagccatc tggttgagcc
    17941 gggtctgaac tggaaaccga cgtttatcga cgaagtcaaa ccggtgaacg tggaagccgt
    18001 gcgtgaactg gccgcttctg gtgtgatgct gacgtcggac gagaacgtag tgcgcgttga
    18061 gatgaacgtg cagtaccgcg tcaccaatcc ggaaaaatat ctgtatagcg tgaccagccc
    18121 ggatgacagc ctgcgtcagg ctaccgacag cgccctgcgt ggagttatcg gtaaatacac
    18181 catggaccgc attctgacgg aaggtcgtac cgtgattcgt agcgatactc agcgcgaact
    18241 ggaagagacg attcgtccgt atgacatggg tatcacgctg ctggacgtca acttccaggc
    18301 tgctcgtccg ccggaagaag taaaagcggc gtttgacgat gcgattgccg cgcgtgaaaa
    18361 cgaacagcaa tacattcgtg aagcagaagc gtattccaac gaagttcagc cgcgtgcgaa
    18421 cggtcaggcg caacgtatcc tcgaagaggc gcgtgcgtac aaggcccaga ccatcctgga
    18481 agctcagggt gaagtggcgc gctttgctaa acttctgccg gaatataaag ccgcgccgga
    18541 aattactcgc gagcgtctgt atatcgagac gatggaaaaa gtgttgggta acacccgcaa
    18601 agtgctggtt aacgataaag gtggcaacct gatggttctg ccgttagacc agatgctgaa
    18661 aggtggtaac gcccctgcgg cgaagagcga taacggtgcc agcaatctgc tgcgtctgcc
    18721 gccagcctct tcctccacaa ccagtggagc aagcaacacg tcgtccacca gtcagggcga
    18781 tattatggac caacgccgcg ccaacgcgca gcgtaacgac taccagcgtc agggggaata
    18841 acgatgcgta agtcagttat cgcgattatc atcatcgtgc tggtagtgct ttacatgtct
    18901 gtctttgtcg tcaaagaagg tgagcgcggt attacgctgc gttttggtaa ggtactgcgt
    18961 gacgatgaca acaaaccttt ggtttatgag cctggtctgc atttcaagat accgttcatt
    19021 gaaacggtga aaatgctcga cgcacgtatt cagaccatgg acaaccaggc cgaccgcttt
    19081 gtgaccaaag agaagaaaga cctgatcgtc gactcttaca tcaaatggcg catcagcgat
    19141 ttcagccgtt actacctggc aacgggtggt ggcgacattt cgcaagcgga agtgctgttg
    19201 aaacgtaagt tctctgaccg tctgcgttct gaaattggtc gcctggacgt gaaagatatc
    19261 gtcaccgatt cccgtggtcg tctgaccctc gaagtacgtg acgcgctgaa ctccggttct
    19321 gcgggtacag aagatgaagt tactaccccg gcagcagata acgccattgc cgaagcggca
    19381 gagcgcgtaa cggctgagac gaagggcaaa gttccggtca tcaacccgaa cagtatggcg
    19441 gcgctgggta ttgaagttgt cgatgtgcgt atcaagcaga tcaacctgcc gaccgaagtg
    19501 tctgaagcga tctacaaccg tatgcgcgcc gagcgtgaag cggtagcgcg tcgtcaccgt
    19561 tcacaaggtc aggaagaagc ggaaaaactg cgcgcgactg ccgactatga agtgaccaga
    19621 acgctggcag aagctgagcg tcagggccgc attatgcgtg gtgaaggcga tgccgaagca
    19681 gccaaactgt ttgctgatgc attcagtaaa gatccggact tctacgcatt catccgtagc
    19741 ctgcgtgctt atgagaacag cttctctggc aatcaggacg tgatggtcat gagcccggat
    19801 agcgatttct tccgctacat gaagacgccg acttccgcaa cgcgttaata taacgactgc
    19861 ggtacaggtc aataaagcca ccgcatcctc agggatgtcg gtggttttct ttttctataa
    19921 ggataatgaa tgaattcgac aatctggctg gcgcttgccc tggttttggt actggaaggt
    19981 ttagggccga tgctttaccc gaaggcatgg aagaagatga tctctgcgat gaccaatttg
    20041 cccgataata ttttacgtcg ttttggcggt ggacttgtgg ttgcgggcgt tgtggtctac
    20101 tacatgttga ggaaaacgat tggctgaaca aaaaacagac tgatcgaggt catttttgag
    20161 tgcaaaaagt gctgtaactc tgaaaaagcg atggtagaat ccatttttaa gcaaacggtg
    20221 attttgaaaa atgggtaaca acgtcgtcgt actgggcacc caatggggtg acgaaggtaa
    20281 aggtaagatc gtcgatcttc tgactgaacg ggctaaatat gttgtacgct accagggcgg
    20341 tcacaacgca ggccatactc tcgtaatcaa cggtgaaaaa accgttctcc atcttattcc
    20401 atcaggtatt ctccgcgaga atgtaaccag catcatcggt aacggtgttg tgctgtctcc
    20461 ggccgcgctg atgaaagaga tgaaagaact ggaagaccgt ggcatccccg ttcgtgagcg
    20521 tctgctgctg tctgaagcat gtccgctgat ccttgattat cacgttgctc tggataacgc
    20581 gcgtgagaaa gcgcgtggcg cgaaagcgat cggcaccacc ggtcgtggta tcgggcctgc
    20641 ttatgaagat aaagtggcac gtcgcggtct gcgtgttggc gaccttttcg acaaagaaac
    20701 cttcgctgaa aaactgaaag aagtgatgga atatcacaac ttccagttgg ttaactacta
    20761 caaagctgaa gcggttgatt accagaaagt tctggatgat acgatggctg ttgccgacat
    20821 cctgacttct atggtggttg acgtttctga cctgctcgac caggcgcgtc agcgtggcga
    20881 tttcgtcatg ttcgaaggtg cgcagggtac gctgctggat atcgaccacg gtacttatcc
    20941 gtacgtaact tcttccaaca ccactgctgg tggcgtggcg accggttccg gcctgggccc
    21001 gcgttatgtt gattacgttc tgggtatcct caaagcttac tcaactcgtg taggtgcagg
    21061 tcctttcccg gccgaactgt ttgatgaaac tggcgagttc ctctgcaagc agggtaacga
    21121 attcggtgca actacgggtc gtcgtcgtcg taccggctgg ctggacaccg ttgccgttcg
    21181 tcgtgcggta cagctgaact ccctgtctgg cttctgcctg actaaactgg acgttctgga
    21241 tggcctgaaa gaggttaaac tctgcgtggc ttaccgtatg ccggatggtc gcgaagtgac
    21301 taccactccg ctggcagctg acgactggaa aggtgtagag ccgatttacg aaaccatgcc
    21361 gggctggtct gaatccactt tcggcgtgaa agatcgtagc ggcctgccgc aggcggcgct
    21421 gaactacatc aagcgtattg aagagctgac cggtgtgccg atcgatatca tctctaccgg
    21481 tccggatcgt actgaaacca tgattctgcg cgacccgttc gacgcgtaat tctggtacgc
    21541 ctggcagata ttttgcctgc cgggctaaca gtgtgataca ttgctgtgtc ggttaagcca
    21601 ttacgctatc cgacacagtg ttaaatcctc gcttttttcc ttccccgaac tgaaataaat
    21661 tagcgacaca gcttgtggct ggtttatcat caatataaat gtattttttc ccgatttccc
    21721 ttttgaggtt gatgtgcagt taacgagttt cactgattac ggattacgtg cgctgatcta
    21781 catggcgtca ttgccagaag ggcggatgac cagtatttct gaagtgactg acgtctacgg
    21841 cgtctcccgt aatcatatgg tcaaaataat caatcaactt agtcgtgccg gctacgtgac
    21901 tgctgttcgt ggaaaaaatg gcggcattcg cctgggtaaa ccggcgagtg cgatacgtat
    21961 tggtgatgtg gtgcgcgagc tggagccctt atcgctggtg aattgcagca gtgagttttg
    22021 ccacattaca cctgcctgtc ggttgaaaca ggcgctttct aaggccgtgc aaagttttct
    22081 tacggaactg gataactaca cgcttgccga tttggttgaa gagaatcaac cgctttataa
    22141 attattgctg gtggagtgac gaaaatcttc atcagagatg acaacggagg aaccgagatg
    22201 tcacaagatc ctttccagga acgcgaagct gaaaaatacg cgaatcccat ccctagtcgg
    22261 gaatttatcc tcgaacattt aaccaaacgt gaaaaaccgg ccagccgtga tgagctggcg
    22321 gtagaactgc acattgaagg cgaagagcag cttgaaggcc tgcgtcgccg cctgcgcgcg
    22381 atggagcgcg atggtcaact ggtcttcact cgtcgtcagt gctatgcgct gccggaacgc
    22441 ctcgacctgg tgaaaggtac cgttattggt caccgtgatg gctacggctt tctgcgggtt
    22501 gaagggcgta aagatgattt gtatctctcc agcgagcaga tgaaaacctg cattcatggc
    22561 gatcaggtgc tggcgcagcc gctgggtgct gaccgtaaag gtcgtcgtga agcgcgtatt
    22621 gtccgcgtac tggtgccaaa aaccagccag attgttggtc gctactttac cgaagcgggc
    22681 gtcggctttg tggttcctga cgatagccgt ctgagcttcg atatcttaat cccgcccgat
    22741 cagatcatgg gcgcgcggat gggctttgtg gtcgtagtcg aactgactca gcgtccgact
    22801 cgccgcacca aagcggtggg taaaatcgtc gaagtgctgg gcgacaatat gggcaccggc
    22861 atggcggttg atatcgctct gcgtacccat gaaattccgt atatctggcc gcaggctgtt
    22921 gagcaacagg ttgcggggct gaaagaagaa gtgccggaag aagcaaaagc gggccgtgtc
    22981 gatttgcgcg atttaccgct ggtcaccatt gatggcgaag acgcccgtga ctttgacgat
    23041 gcagtttact gcgagaaaaa acgcggcggc ggctggcgtt tatgggtcgc gattgccgac
    23101 gtcagctact atgtgcgtcc gccaacgccg ctggacagag aagcgcgtaa ccgtggcacg
    23161 tcggtgtact tcccttcgca ggttatcccg atgctgccgg aagtgctctc taacggcctg
    23221 tgttcgctca acccgcaggt agaccgcctg tgtatggtgt gcgagatgac ggtttcgtcg
    23281 aaaggccgcc tgacgggcta caaattctac gaagcggtga tgagctctca cgcgcgtctg
    23341 acctacacca aagtctggca tattctgcag ggcgatcagg atctgcgcga gcagtacgcc
    23401 ccgctggtta agcatctcga agagttgcat aacctctata aagtgctgga taaagcccgt
    23461 gaagaacgcg gtgggatctc atttgagagc gaagaagcga agttcatttt caacgctgaa
    23521 cgccgtattg aacgtatcga acagacccag cgtaacgacg cgcacaaatt aattgaagag
    23581 tgcatgattc tggcgaatat ctcggcggcg cgtttcgttg agaaagcgaa agaaccggca
    23641 ctgttccgta ttcacgacaa gccgagcacc gaagcgatta cctctttccg ttcagtgctg
    23701 gcggagctgg ggctggagct gccgggtggt aacaagccgg aaccgcgtga ctacgcggaa
    23761 ctgctggagt cggttgccga ccgtcctgat gcagaaatgc tgcaaaccat gctgctacgc
    23821 tcgatgaaac aggcgattta cgatccagaa aaccgtggtc acttcggtct ggcattgcag
    23881 tcctatgcgc actttacttc gccgattcgt cgttatcctg acctgacgct gcaccgcgcc
    23941 attaaatatc tgctggcgaa agagcagggg catcagggca acaccactga aaccggcggc
    24001 taccattatt cgatggaaga gatgttgcaa ctgggtcagc actgttcgat ggcggaacgt
    24061 cgtgccgacg aagcaacgcg cgatgtcgct gactggctga agtgtgactt catgctcgac
    24121 caggtaggta acgtctttaa aggcgtaatt tccagcgtca ctggctttgg cttcttcgtc
    24181 cgtctggacg acttgttcat tgatggtctg gtccatgtct cttcgctgga caatgactac
    24241 tatcgctttg accaggtagg gcaacgcctg atgggggaat ccagcggcca gacttatcgc
    24301 ctgggcgatc gcgtggaagt tcgcgtcgaa gcggttaata tggacgagcg caaaatcgac
    24361 tttagtctga tctccagtga acgcgcaccg cgcaacgtcg gtaaaacggc gcgcgagaaa
    24421 gcgaaaaaag gcgatgcagg caaaaaaggc ggcaagcgtt gtcaggtcgg taaaaaggta
    24481 aactttgagc cagacagcgc cttccgcggt gagaaaaaaa cgaagccgaa agcggcgaag
    24541 aaagacgcga gaaaagcgaa aaagccatcg gcgaaaacgc agaaaatagc cgcagcgacc
    24601 aaagcgaagc gtgcggcgaa gaaaaaagtg gcagagtgat caataccctc tttaaaagaa
    24661 gagggttaga ttgctgacaa aatgcgcttt gttcatgccg gatgcggcgt gaacgcctta
    24721 tccggcctac ataatcacgc aaattcaata tattgcagag atcatgtagg ccggataaac
    24781 gtagtgcatc aggcaaaacg taaacaacga gtacattaat gagcgaaatg atttacggca
    24841 tccacgcagt gcaggccctg ctggagcgcg cccctgaacg ttttcaggaa gtctttattt
    24901 taaaaggccg tgaagataaa cgtctgttac cgctgattca cgcccttgag tctcagggcg
    24961 tggttatcca gttggcaaac cgccaatatc tcgacgagaa aagcgacggt gccgtgcatc
    25021 agggcattat cgcccgcgtg aagccaggac gtcagtatca ggaaaacgat ctgccggatc
    25081 tgatcgcttc gctcgatcaa ccgttcctgc tgatcctcga cggcgtaacc gatccgcaca
    25141 acctcggcgc gtgcctgcgt agtgcggacg ccgcaggcgt tcatgcggtg attgtgccga
    25201 aagatcgctc cgcacagctc aacgctacgg cgaaaaaagt agcctgcggc gcggcagaaa
    25261 gcgttccgct gattcgggtg actaaccttg cgcgcaccat gcgtatgttg caggaagaga
    25321 atatctggat cgtcggtacg gcaggcgagg cggatcatac actctatcag agcaaaatga
    25381 ccggacgcct ggcgctggtg atgggcgcgg aaggtgaagg tatgcgtcgc ctgacccgtg
    25441 aacattgcga tgagttgatc agcatcccaa tggcaggaag tgtttcttcc ctgaacgttt
    25501 cggttgcaac cggaatttgc ttatttgaag cggttcgtca gcgcagctag ttcctctgaa
    25561 ttatgaaaag ccatccagat ttggatggtt
//
LOCUS       DAZSIO010000044        24960 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZSIO010000044 DAZSIO010000000
VERSION     DAZSIO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24960
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.044"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            168..1271
                     /gene="proB"
                     /locus_tag="ACHX5T_001646"
     CDS             168..1271
                     /gene="proB"
                     /locus_tag="ACHX5T_001646"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR8466886.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1283..2536
                     /gene="proA"
                     /locus_tag="ACHX5T_001647"
     CDS             1283..2536
                     /gene="proA"
                     /locus_tag="ACHX5T_001647"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR8466887.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2651..2726
                     /locus_tag="ACHX5T_001648"
     tRNA            2651..2726
                     /locus_tag="ACHX5T_001648"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2684..2686,aa:Thr,seq:cgt)
     gene            complement(2741..3895)
                     /locus_tag="ACHX5T_001649"
     CDS             complement(2741..3895)
                     /locus_tag="ACHX5T_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR8466888.1"
                     /translation="MKLRGGTWHCDFVAPDGSRVRRSLETSDKRQAQELHDRLKAEAW
                     RVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWMLHFREAILSDITTEKIM
                     EAVDGMENRRHRLNWEMSRDRCLRLGNPVPEYKPKLASKGTKTRHLAILRAILNMAVE
                     WGWLDRAPKISTPRVKNGRIRWLTEEESKRLFAEIAPHFFPVVMFAITTGLRRSNVTD
                     LEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQGLHKRWVFVHTKPAYR
                     SDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWASWLVQSGVSLLALKEM
                     GGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNTAQEENVVYLNAR"
     gene            complement(3778..4125)
                     /locus_tag="ACHX5T_001650"
     CDS             complement(3778..4125)
                     /locus_tag="ACHX5T_001650"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8466889.1"
                     /translation="MEEEIFTREEAASYLKVDKGTITQWIRSGRLQAAKINPDKPKSP
                     YRICKSDCIAALKSVRHNSAVNAVDVQEVKACQSNYAVARGTAISSRQMDQELDALLK
                     HRTKGKRKNFTIV"
     gene            complement(4212..4556)
                     /locus_tag="ACHX5T_001651"
     CDS             complement(4212..4556)
                     /locus_tag="ACHX5T_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466890.1"
                     /translation="MSDLAMKVLKWQSTDDVGISSATLASIACGLKKNIYGHSFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            complement(4657..4836)
                     /locus_tag="ACHX5T_001652"
     CDS             complement(4657..4836)
                     /locus_tag="ACHX5T_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eag protein"
                     /protein_id="HFR8466891.1"
                     /translation="MSCPKCGSGNIAKEKTMRGWSGDYVCCDCGYNDSKDAFGERGKN
                     EFVKINKEREGNEKS"
     gene            complement(4933..5862)
                     /locus_tag="ACHX5T_001653"
     CDS             complement(4933..5862)
                     /locus_tag="ACHX5T_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR8466892.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVAY
                     IIQDSDARSRGEKGILRYFANISDEDINEYEITVTPLYAAPPETVSVPDAMEMDDDFD
                     SAFEHGKAVGWNAYRAAMLQSGNFRENKNSSTNNFREIAETSTNYPVIPSEVLSAIQK
                     VAKIRADFDDFDGDRRGIGDCLDEAEQELIVTINKYASQLAAEPIAPNDVREQTAIPQ
                     VPVTPDGWISCSERMPVIGELNWRTSFPLLITCEIGVIPAYYGFVSVNGNKHYGFMES
                     LKYGDDNGNHPQTNEYGLISNVTHWMPLPEPPL"
     gene            complement(5865..6056)
                     /locus_tag="ACHX5T_001654"
     CDS             complement(5865..6056)
                     /locus_tag="ACHX5T_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466893.1"
                     /translation="MDGQISIVRPGACDDREIRLIIRLAMGKTITALITPENLALALT
                     GKSDLPVELKLRNIEIKVK"
     gene            complement(6058..6687)
                     /locus_tag="ACHX5T_001655"
     CDS             complement(6058..6687)
                     /locus_tag="ACHX5T_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFR8466894.1"
                     /translation="MSEINYQALREAAERAIPAMERLLMLPADDLLSEQELKDYGVDI
                     DALNAFKFLAGPETVLALLDERVRNQQYIKSRDQENEDIALTVGKLLIENGRLVADTL
                     RHLADNEIDSDYFAITSTNENGTEIDHEMAITDYALQAAGTVDELVAALESAENRIAE
                     LGERGILLPERSSMLHRTDFHDDYQTVMAYKVSEVIAAIRAAGIRIKGE"
     gene            complement(6684..7259)
                     /locus_tag="ACHX5T_001656"
     CDS             complement(6684..7259)
                     /locus_tag="ACHX5T_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001701210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hNH endonuclease"
                     /protein_id="HFR8466895.1"
                     /translation="MKQMSLIEMDGFLKGKCIPCDLKVNETNAEYLVRKFAEAEAKIS
                     ALYEDHQKAIESIKQADAAVKLAHEKFSALAAENAGMKSVIEYCINPDNQPEYHDQGM
                     GCGVEDHGYQRDGYSACYYGWESAMDRVYSEVIPDAIPETPATDTFLDEVRAQGVEMF
                     AASMKVVGGHEHPYSAMANEFAAQLRKGGGQ"
     gene            complement(7256..7420)
                     /locus_tag="ACHX5T_001657"
     CDS             complement(7256..7420)
                     /locus_tag="ACHX5T_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFR8466896.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRKSFGSVNDNKY
                     LNAMWRSGKK"
     gene            complement(7431..7724)
                     /locus_tag="ACHX5T_001658"
     CDS             complement(7431..7724)
                     /locus_tag="ACHX5T_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HFR8466897.1"
                     /translation="MPAPLYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEELERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(7748..8131)
                     /locus_tag="ACHX5T_001659"
     CDS             complement(7748..8131)
                     /locus_tag="ACHX5T_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466898.1"
                     /translation="MAHSITVRLNKPAREFQAGENIGFNIRAGVQYYDRQTKKKEWTN
                     YSAVVFAKPGAQADYYRSVLVEGGIVEITGENIRVDVYQGQNGQSITLELLNAKIGFA
                     TSGNSQQQQSSNHQNHPEYDDSIPF"
     gene            complement(8131..8736)
                     /locus_tag="ACHX5T_001660"
     CDS             complement(8131..8736)
                     /locus_tag="ACHX5T_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFR8466899.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAALQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDIYNIRRDELEGAAA"
     gene            complement(8993..9145)
                     /gene="kil"
                     /locus_tag="ACHX5T_001661"
     CDS             complement(8993..9145)
                     /gene="kil"
                     /locus_tag="ACHX5T_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFR8466900.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINI
                     PWYRRK"
     gene            complement(9130..9264)
                     /locus_tag="ACHX5T_001662"
     CDS             complement(9130..9264)
                     /locus_tag="ACHX5T_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466901.1"
                     /translation="MMHFQLAGSGVMSAFYPHESELSRRVKQLISAAKKQLEALCAMK
                     "
     gene            complement(9459..9929)
                     /locus_tag="ACHX5T_001663"
     CDS             complement(9459..9929)
                     /locus_tag="ACHX5T_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466902.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQKRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSTPDIEYNFGHNQETENWVFY"
     gene            complement(9938..10264)
                     /locus_tag="ACHX5T_001664"
     CDS             complement(9938..10264)
                     /locus_tag="ACHX5T_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFR8466903.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRIERKNQRTWYSKPRSEMGVTCVGRQKMKL
                     GSKPLI"
     gene            complement(10745..10939)
                     /locus_tag="ACHX5T_001665"
     CDS             complement(10745..10939)
                     /locus_tag="ACHX5T_001665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466904.1"
                     /translation="MNGDRYEFTKDSTEDSVFHVTINGDKSSVYESVSGVYPEMKYTA
                     LSSNTMVGEYQSGGGITVET"
     gene            complement(11029..11661)
                     /locus_tag="ACHX5T_001666"
     CDS             complement(11029..11661)
                     /locus_tag="ACHX5T_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFR8466905.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            11765..11980
                     /locus_tag="ACHX5T_001667"
     CDS             11765..11980
                     /locus_tag="ACHX5T_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8466906.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            12097..12378
                     /locus_tag="ACHX5T_001668"
     CDS             12097..12378
                     /locus_tag="ACHX5T_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015995142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFR8466907.1"
                     /translation="MGTIATKSKKAARIESALLNKLALMGQKTFARAMGVPEYQVSRW
                     KNGFFSQVSMMLAVLEYGIEDDEMAELTKRLADYLTKEKAPKNGEFFEA"
     gene            12413..12574
                     /locus_tag="ACHX5T_001669"
     CDS             12413..12574
                     /locus_tag="ACHX5T_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466908.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            12561..13391
                     /locus_tag="ACHX5T_001670"
     CDS             12561..13391
                     /locus_tag="ACHX5T_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFR8466909.1"
                     /translation="MGVVKLADYRPQLEVVEHRVAELEDGYTRTANTLLEAAMLSGLT
                     LHQLLIVMAVWRKTYGYNKKIDWIGNEQFAELTGMAPTKCSTAKNELIRMGVLIQVGR
                     QVGMNTNISEWKTKVNGFGKTFTNSVKQTFTKSVKSNLPNQSNTKDNIQKTINTNTPL
                     PPNGGGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQL
                     KTPNVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA
                     "
     gene            13388..14764
                     /locus_tag="ACHX5T_001671"
     CDS             13388..14764
                     /locus_tag="ACHX5T_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR8466910.1"
                     /translation="MKQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIDELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTASNAPAARGR
                     RYAQGADV"
     gene            14837..15163
                     /locus_tag="ACHX5T_001672"
     CDS             14837..15163
                     /locus_tag="ACHX5T_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466911.1"
                     /translation="MADWQIPIIILAGASLVAGFILLKKHKDRDQKVEVLYGYPANST
                     TWLTIYHYRKSGRWVFEWDDLFAEKRPKSWGDISECMMFEERKSGATREEFNEAWARL
                     SERGYL"
     gene            15160..15360
                     /locus_tag="ACHX5T_001673"
     CDS             15160..15360
                     /locus_tag="ACHX5T_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466912.1"
                     /translation="MSKYEKLDQNILSMLSERPTPVFDIWLKWRSNGMYIETIDRRMQ
                     YLRKKGLVANVRGKGWVKINLS"
     gene            15372..15560
                     /locus_tag="ACHX5T_001674"
     CDS             15372..15560
                     /locus_tag="ACHX5T_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004904153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466913.1"
                     /translation="MDDSLEIFEEWCADELGVSAYFIRQMRSKNILGVIEYKRVEINK
                     RYRAWMAAVRAAGVKVKE"
     gene            15563..15727
                     /locus_tag="ACHX5T_001675"
     CDS             15563..15727
                     /locus_tag="ACHX5T_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466914.1"
                     /translation="MSAYEEIMLALRFFFGVEEDENVNEIIGQDHDPIGTIAAALDDY
                     RSVNGEETNV"
     gene            15708..16148
                     /locus_tag="ACHX5T_001676"
     CDS             15708..16148
                     /locus_tag="ACHX5T_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFR8466915.1"
                     /translation="MRKLTFELRSPIHQQNAIQAIQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGRSTS
                     RMRVGEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDKTE"
     gene            16145..16735
                     /locus_tag="ACHX5T_001677"
     CDS             16145..16735
                     /locus_tag="ACHX5T_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFR8466916.1"
                     /translation="MNKYRLIYADPPWQYRDKANDGNRGAGHKYDVMNVQDICRLPVW
                     DLADPESCLLAMWWVPTQPAEALKVIEAWGFRLMTMKGFTWHKTNKHKGNSAIGMGHM
                     TRANSEDCLFAVRGRLPERMDASICQHFTAPRMEHSAKPPIVRDMLAKLLGDVPRCEL
                     FSRDKVTGWDMWGNQCDSDFELAPGMAIKPCKMVIA"
     gene            16732..16905
                     /gene="ninD"
                     /locus_tag="ACHX5T_001678"
     CDS             16732..16905
                     /gene="ninD"
                     /locus_tag="ACHX5T_001678"
                     /inference="COORDINATES: protein motif:HMM:NF036649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinD"
                     /protein_id="HFR8466917.1"
                     /translation="MKHCYRCGERKEDDRFRPGQPYWNRWCLRCERTPTGVLPLPQEK
                     EDVWRDSDEVSPT"
     gene            16872..17047
                     /locus_tag="ACHX5T_001679"
                     /pseudo
     CDS             16872..17047
                     /locus_tag="ACHX5T_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
     gene            17050..17451
                     /locus_tag="ACHX5T_001680"
     CDS             17050..17451
                     /locus_tag="ACHX5T_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466918.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMMSIRLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVENHHAVRQAKRKANMTYMKAYRKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
     gene            17411..17641
                     /locus_tag="ACHX5T_001681"
     CDS             17411..17641
                     /locus_tag="ACHX5T_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFR8466919.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEHCCAELMSDPNSSMYEEEDDGQTSAKTM"
     gene            17613..18224
                     /locus_tag="ACHX5T_001682"
     CDS             17613..18224
                     /locus_tag="ACHX5T_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFR8466920.1"
                     /translation="MAKPARRRCKNEECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIG
                     QEAVDEIESNHNRHRWTVVECKAIKAEYQQKLKDLRNSRSEAA"
     gene            18221..18427
                     /locus_tag="ACHX5T_001683"
     CDS             18221..18427
                     /locus_tag="ACHX5T_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFR8466921.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            18405..19070
                     /locus_tag="ACHX5T_001684"
     CDS             18405..19070
                     /locus_tag="ACHX5T_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFR8466922.1"
                     /translation="MRYYEKIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NIDYDKEILAKALSHKADELPLIIELVSKDKKYVICHADYPCDEYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            19067..19690
                     /locus_tag="ACHX5T_001685"
     CDS             19067..19690
                     /locus_tag="ACHX5T_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR8466923.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            20123..20446
                     /locus_tag="ACHX5T_001686"
     CDS             20123..20446
                     /locus_tag="ACHX5T_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFR8466924.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            20430..20906
                     /locus_tag="ACHX5T_001687"
     CDS             20430..20906
                     /locus_tag="ACHX5T_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFR8466925.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            20903..21340
                     /locus_tag="ACHX5T_001688"
     CDS             20903..21340
                     /locus_tag="ACHX5T_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR8466926.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKEQRDKAASTI
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLK"
     gene            21545..22087
                     /locus_tag="ACHX5T_001689"
     CDS             21545..22087
                     /locus_tag="ACHX5T_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR8466927.1"
                     /translation="MNYPTVVNDIDFRDLIFVANNDPVTDSFMVAKAFGKLPKNVVRD
                     IERTIEACPPEFDTKLNFELCYKNNELQNGKPQKFYRLRKDGLMLLVMSDTKKEAMRI
                     KIAYINAFNWMYAMLQVGHRQFEEERNAVMLEYMKEKDVASMSGRLLNRWGKIKKPQL
                     MARIERLEQHGQTVIPGLIN"
     gene            22307..22522
                     /locus_tag="ACHX5T_001690"
     CDS             22307..22522
                     /locus_tag="ACHX5T_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR8466928.1"
                     /translation="MDDNGNQWVNVEHRLPESKEGMWSKEVIALTDTGDVFKLSCMGS
                     YWQRTKAFIDSGASKVTHWMPLNYPDD"
     gene            22670..22912
                     /locus_tag="ACHX5T_001691"
     CDS             22670..22912
                     /locus_tag="ACHX5T_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HFR8466929.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITIRTFEAVRKSKEALDLFTTGA"
     gene            22948..23436
                     /locus_tag="ACHX5T_001692"
     CDS             22948..23436
                     /locus_tag="ACHX5T_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFR8466930.1"
                     /translation="MAAPKGNRFWEARSSHGRNPKFESPEALWAACCEYFEWADDNPL
                     WEGKVFSYQGEIIKANVPKMRAMTISGLCTFLDITRQTWGTFRSMEGFSDVTSRAEDI
                     IYDQKFSGAAADLLNANIIARDLGLKEQSQVEDVTPDKGDRDKRRSRIKELFNRGTGR
                     DS"
     gene            23414..24913
                     /locus_tag="ACHX5T_001693"
     CDS             23414..24913
                     /locus_tag="ACHX5T_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR8466931.1"
                     /translation="MELDAILDNLSDEEQIELLELLEEEENYRNTHLLYEFTPYSKQR
                     EFIDAGHDYPERCFMAGNQLGKSFTGAAEVAFHLTGRYPGTKGYPADGKYGGEWKGKR
                     FYEPVVFWIGGETNETVTKTTQRILCGRIEENGEPGYGSIPKEDIISWKKSPFFPNLV
                     DHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGVWFDEEPPYSIYGEGLTRT
                     NKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYDAEHYTDEQKEQIIASYP
                     EHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVIGGMDFGWDHPQAQVQLW
                     WDKDADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTAWPHDGNQHEKGGGEQLKGQY
                     ADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFKVFNTCEPFFEEFRLYHRDEN
                     GKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKIPAPIRPIARRT"
BASE COUNT         6677 a         5878 c         6109 g         6296 t
ORIGIN      
        1 tggtaattta ttaaatctgt aataaaagcg taaacaactg ccgctacgct tgctgatccc
       61 gcgcaacaaa acgccatgct ttgctcgcag atggttggca accgacgaca gtcctgctaa
      121 aacgttcgtt tgatatcatt tttcctaaaa ttgaatggca gagaatcatg agtgacagcc
      181 agacgctggt ggtaaaactc ggcaccagtg tgctaacagg cggatcgcgc cgtctgaacc
      241 gtgcccatat cgttgaactt gttcgccagt gcgcgcagtt acatgccgcc gggcatcgga
      301 ttgttattgt gacgtcgggc gcgatcgccg ccggacgtga gcacctgggt tacccggaac
      361 tgccagcgac tatcgcctcg aaacaactgc tggcggcggt agggcagagt cgactgattc
      421 aactgtggga acagctgttt tcgatttatg gcattcacgt cgggcaaatg ctgctgaccc
      481 gtgctgatat ggaagaccgt gaacgcttcc tgaacgcccg cgacaccctg cgtgcgttgc
      541 tcgataacaa tatcgttccg gtaatcaatg agaacgatgc tgtcgctacg gcagagatta
      601 aggtcggcga taacgataac ctttctgcgc tggcggcgat tctggcgggt gccgataaac
      661 tgttgctgct gaccgatcaa aaaggtttgt ataccgctga tccgcgcaga aatccgcagg
      721 cagaactgat taaagatgtt tacggcattg atgacgcact gcgcgcgatt gccggtgaca
      781 gcgtttcagg cctcggaact ggcggcatga gtactaaatt gcaggccgct gacgtggctt
      841 gccgtgcggg tatcgacacc attattgccg cgggcagcaa gccgggcgtt attggtgatg
      901 tgatggaagg catttccgtc ggtacgctgt tccatgccca ggcgactccg cttgaaaacc
      961 gtaaacgctg gattttcggt gcgccgccgg cgggtgaaat cacggtagat gaaggggcaa
     1021 ctgccgccat tctggaacgc ggcagctccc tgttgccgaa aggcattaaa agcgtgactg
     1081 gcaatttctc gcgtggtgaa gtcatccgca tttgcaacct cgaaggtcgc gatatcgccc
     1141 acggtgtcag tcgttacaac agcgatgcat tacgccgtat tgccggacac cactcgcaag
     1201 aaattgatgc aatactggga tatgaatacg gcccggttgc cgttcaccgt gatgacatga
     1261 ttacccgtta aggagctggc cgatgctgga acaaatgggc attgccgcga agcaagcctc
     1321 gtataaatta gcgcaactct ccagccgcga aaaaaatcgc gtgctggaaa aaatcgccga
     1381 tgaactggaa gcacaaagcg aaatcatcct caacgctaac gcccaggatg ttgctgacgc
     1441 gcgagccaat ggccttagcg aagcgatgct tgaccgtctg gcactgacgc ccgcacggct
     1501 gaaaggcatt gccgacgatg tacgtcaggt gtgcaacctc gccgatccgg tggggcaggt
     1561 aatcgatggc ggcgtactgg acagcggcct gcgtcttgag cgtcgtcgcg taccgctggg
     1621 ggttattggc gtgatttatg aagcgcgccc gaacgtgacg gttgatgtcg cttcgctgtg
     1681 cctgaaaacc ggtaatgcgg tgatcctgcg tggtggtaaa gaaacctgtc gcactaacgc
     1741 ggcaacggtg gcggtgattc aggacgccct gaaatcctgt ggcttaccgg cgggtgccgt
     1801 gcaggcgatt gataatcctg accgtgcgct ggtcagtgaa atgctgcgta tggataaata
     1861 catcgacatg ctgatcccgc gtggtggcgc tggtttgcat aaactgtgcc gtgaacagtc
     1921 gacaatcccg gtgatcacag gtggtatagg cgtatgccat atttacgttg atgaaagtgc
     1981 agagatcgct gaagcattaa aagtgatcgt caacgcgaaa actcagcgtc cgagcacatg
     2041 taatacggtt gaaacgttgc tggtgaataa aaacatcgcc gatagcttcc tgcccgcatt
     2101 aagcaaacaa atggcggaaa gcggcgtgac attacacgca gatgcagctg cgctgacaca
     2161 gttgcaggca ggccctgcga aggtggtggc ggttaaagcc gaagagtatg acgatgagtt
     2221 tctgtcatta gatttgaacg tcaaaatcgt cagcgatctt gacgatgcca tcgcccatat
     2281 tcgtgaacac ggcacacaac actccgatgc gatcctgacc cgcgatatgc gcaacgccca
     2341 gcgttttgtt aacgaagtgg attcgtccgc tgtttacgtt aacgcctcta cgcgttttac
     2401 cgacggcggc cagtttggtc tgggtgcgga agtggcggta agcacacaaa aactccacgc
     2461 gcgtggccca atggggctgg aagcactgac cacttacaag tggatcggca ttggtgatta
     2521 caccattcgt gcgtaaataa aaccgggtga tgcaaaagta gccatttgat tcacaaggcc
     2581 attgacgcat cgcccggtta gttttaacct tgtccaccgt gattcacgtt cgtgaacatg
     2641 tcctttcagg gccgatatag ctcagttggt agagcagcgc attcgtaatg cgaaggtcgt
     2701 aggttcgact cctattatcg gcaccattta aatcaataag ttacctcgca tttaagtaaa
     2761 ccacgttctc ctcttgtgcc gtatttgtgc cattgcgact tataatcgca tcgattttgc
     2821 ttgcgtgctc ggtgagatgc ccggctgaaa ggtgggcgta tctttgaacc atttcgagag
     2881 tttcccatcc tcccatctct ttaagtgcaa gaagagagac accggactga accagccagc
     2941 ttgcccaggt atgcctcaga tcatggaagc ggaagttgct aatgcctgcc cgctttaacg
     3001 ctcccttcca tgccttgttg ctgtcggttc tcatcttcct taccgctgct gtttttgttc
     3061 cgtcgcttcg gtaggcaggt ttggtgtgga caaataccca tctcttatgg agcccctgct
     3121 gttttcttaa tatctggcat gcggtttcgt taagaggaac tccgatcgca ttgccagctt
     3181 ttgtttcatc agggtgcatc catgccattt tcttatccag atcgacctgt gaccactcaa
     3241 ggtctgtaac gttggaacgg cgaaggcctg tcgtgattgc aaacatgacc acagggaaga
     3301 agtgaggagc aatttctgca aacaggcgct tcgattcctc ctctgtaagc catctgatgc
     3361 gtccattctt aacgcgtggt gttgatattt tgggtgccct gtcaagccat ccccattcaa
     3421 cagccatatt gagaatggcg cgaagtattg ccagatgcct cgtcttcgtt cctttgcttg
     3481 caagctttgg tttatactcc ggcaccggat tgccaagtcg caaacacctg tcccggctca
     3541 tctcccagtt caggcgatgg cggcggtttt ccatcccgtc taccgcctcc attatttttt
     3601 ctgttgttat gtcagagaga atggcttctc tgaagtgcaa catccagaac gatataatgc
     3661 tcttgtcatc atcaatggac ttcttatccg atttctcacg cagccaccgt atgcaggctt
     3721 ccttgaatag ctttttcggt gattccccga gattttttac tctccacgct tctgctttca
     3781 gacgatcgtg aagttcttgc gcttgccttt tgtccgatgt ttcaagagag cgtctaactc
     3841 ttgatccatc tggcgcgacg aaatcgcagt gccacgtgcc accgcgtagt ttgattgaca
     3901 tgctttaacc tcctgcacat caaccgcatt caccgcgcta ttgtgtctca cagacttaag
     3961 cgccgcaatg cagtctgact tgcaaatgcg atatgggctt ttaggtttat ctggatttat
     4021 ctttgcggcc tgaagtcgtc cacttcgtat ccactgcgtg atagtgcctt tgtctacctt
     4081 cagatacgac gctgcctctt cacgagtgaa gatttcttct tccacctgga atctccattt
     4141 attggattgg tattattgcg gtaggtctgg atatcattga gcaatgaaca ggcctcatcg
     4201 agtgtgaggc gttagtcctt gcgtagctcg ctgattcttc tgtaagtctc tggtgctttg
     4261 ttcccgtacg tcttcatttc agacttcaac agagcaacga gtgaatccca ttcgttgagg
     4321 attcctttga atgccggaac gcgctttgca accttgtcga atgaatctct gatttctgga
     4381 atctgctcaa caagtgcaac gcatcgccgg aaatcggctg cgtcatgtgg agcaccgaag
     4441 ctatgaccat agatattctt tttcagtcca catgcgattg aggcaagagt tgcgctactg
     4501 atgccgacat cgtcagtcga ttgccatttc aaaaccttca tagccaaatc tgacatttct
     4561 tgtctccaat aaaaaaccgc catcaggcgg cttggtgttc tttcaattct tcaattcgaa
     4621 tattggttac attgttttca tatatgaata aataaattag cttttttcgt tgccttcgcg
     4681 ttctttatta attttgacaa actcgttttt accacgctct ccaaatgcgt ctttagagtc
     4741 gttgtatccg caatcgcagc acacataatc accagaccat ccacgcattg ttttttcttt
     4801 tgcaatattt ccagaaccgc attttggaca agacatatca ctacctccaa agcatgagtg
     4861 agatgacaac gtaacattga ttggagatta acaatagatt gctgatgtaa aagatatgta
     4921 taagcttcgc tttcaaagtg gaggctctgg tagcggcatc cagtgggtta cattgctaat
     4981 cagaccatat tcattagttt gaggatggtt gccgttatcg tctccgtatt taagactctc
     5041 cataaaacca taatgcttat tcccattaac gctcacaaag ccgtaataag ctggtataac
     5101 gccgatctca cacgtaatca gcaaaggaaa actagttctc caatttagct cgccgattac
     5161 aggcattctc tcactacagc ttatccaacc atccggagtt accggaactt gcggaatggc
     5221 tgtctgctct cgaacgtcat taggcgcgat aggttctgct gccaactgac tggcatattt
     5281 gttaatggta acgataagct cttgctcagc ctcatccaga caatcaccga tacctcgcct
     5341 gtcaccgtca aaatcatcga aatcggcacg aatcttggca accttctgga ttgcggacaa
     5401 cacctcacta ggaattaccg gatagttggt tgacgtttcc gcgatttcac gaaaattatt
     5461 ggttgacgaa ttcttgtttt cccgaaagtt tccggactga agcatggctg cgcgataggc
     5521 gttccagccg acagcttttc cgtgttcaaa cgcgctgtca aagtcatcat ccatttccat
     5581 cgcatcaggc acagataccg tctctggagg ggcggcgtaa agcggagtta cggtaatttc
     5641 gtactcgttt atatcctcgt cgctgatatt tgcgaaatag cgaaggatgc ctttctctcc
     5701 acgactacga gcgtcagagt cctgaatgat atacgccaca ggctctgctt ccagcgatgc
     5761 cagcgcaatt cgtgccagtt ccatttgttc gccacgagta agcccgtttt caagcggatt
     5821 tttaatgaac aattcaatac gttctttggt aatagtggtc atagctattt caccttaatc
     5881 tcaatatttc gcagctttag ctctactggc aggtctgact ttcctgttaa cgctaatgcg
     5941 agattttctg gagtaatgag agcagttatt gttttcccca tcgccagacg aataatcagt
     6001 cgtatctcgc gatcgtcaca tgctcctggt cgaacaattg atatttgtcc gtccatctca
     6061 ctctcctttg atgcgaatgc cagcggcgcg gattgcagcg atgacctcag aaactttgta
     6121 tgccattacc gtttggtaat catcgtgaaa atctgttcga tgaagcatgc tgctacgttc
     6181 cgggagcagt attccccgct cccccagttc tgctatgcga ttctctgcgg attccagcgc
     6241 cgcaaccaat tcgtctacag ttccggcagc ttgcagtgcg taatcggtaa tagccatctc
     6301 atgatcaatt tcagtaccgt tctcattcgt tgaggtgata gcaaaataat cagagtcgat
     6361 ttcgttatca gctaagtggc gtagcgtatc ggcaacaagc cggccgtttt cgattagcag
     6421 cttccctacc gttagcgcaa tatcctcgtt ctcctggtcg cgggatttga tgtattgctg
     6481 attccttacc cgttcatcca gtagtgccag cacggtttct ggtccggcca gaaatttgaa
     6541 ggcgttgagc gcatcaatat ccacaccgta atctttaagt tcctgttcac ttaacaaatc
     6601 atcagctggc aacattaaca ggcgttccat tgctggaatt gcacgttccg ccgcctcacg
     6661 cagtgcctga tagttaattt cgctcactgg ccgcctcctt tgcgaagctg ggcggcgaac
     6721 tcgttagcca tcgctgaata tgggtgctca tgaccgccaa caaccttcat gctggcagca
     6781 aacatctcca cgccctgcgc ccgcacttca tccaggaaag tgtcggtggc tggggtttct
     6841 ggaatggcgt ctggaataac ctctgagtag acgcgatcca ttgccgactc ccatccgtag
     6901 taacaagccg aatagccgtc gcgctggtag ccgtggtctt caactccaca tcccatgccc
     6961 tggtcatggt attcaggctg attgtcggga ttaatgcagt attcaatgac cgacttcatc
     7021 cccgcattct ccgccgccag cgccgaaaac ttctcgtgtg ccaacttaac agccgcatca
     7081 gcctgcttaa ttgactcaat cgctttctgg tggtcttcgt acagagccga aatcttggcc
     7141 tccgcttcag caaatttacg caccagatat tcagcgtttg tttcgttaac ctttaaatca
     7201 catgggatgc atttaccttt cagaaaacca tccatctcaa ttagtgacat ttgtttcatt
     7261 tcttcccact ccgccacata gcattcagat atttgttgtc attaacagaa ccgaaactct
     7321 ttctcttaag taattcctct ctcgatggca ttggctttac gcgttggcga ataatcattt
     7381 ctgccggaag aatgccggga ttgtatgcaa gtcctctcat ggtaaattcc tcagtcatta
     7441 ctgatagcgc catagcgtga gcggtaatta cgcaggcgcg ggtcaatttc agggaagtgg
     7501 gtatatgtgg ctttgcggaa tggtcggatt gatgtctggt aaattcgctc gagttcttct
     7561 ttctctgcaa gccatataca gtggcgaaat tccttttcct ctttcgtttc ctgcggtagc
     7621 gacattatcc gatcgtagtt ttttctgaat ttatccagca cctccgatac ggaattgccg
     7681 gaacagcggc gtgggtcatc cgcaccatac agaggcgctg gcataatgga atccttatgt
     7741 tgctacttta gaagggaatt gaatcgtcgt attcaggatg attttgatga ttgctacttt
     7801 gctgctgttg gctgtttcct gaagttgcaa atccaatctt tgcattcagt aattcaagag
     7861 tgattgattg tccattttgc ccctgataaa catcaaccct gatgttttct ccggtaattt
     7921 ccacaatgcc accttcaaca agaacactac ggtagtaatc cgcttgcgct cccggcttgg
     7981 caaatacaac ggcgctgtag tttgtccatt ctttcttttt tgtctggcga tcgtaatact
     8041 gaacgccagc acggatgttg aatccgatat tttccccggc ctgaaactct cttgcgggct
     8101 tgtttagtct tacagtaatc gaatgtgcca ttaagcagcc gctccttcta attcgtctcg
     8161 tctgatgttg taaatgtcct gcgctttgtg ctgctccggt gtgccttcga gcatcttcca
     8221 cgctttggcg aacgcctgtt taagctcttc cacggtgttt ttctgcaatg ctgcgtcagt
     8281 gaatgctttt agaacctgtt caggtgtagg tgatggtttt gattgctttg ctgctgcgtt
     8341 ctgctgatgt ttatgctcgt cggtatctgc atctttcgca tcatcaatgc cgaacaaacc
     8401 attgaggcaa tacttgcgtg cataagagct tgtggctccc gtaacttgtg cagaatccat
     8461 tcctttcttg ctttcttcct ctcgtgcaag agcggttgcc gtatgactgt tttcgccatc
     8521 ggtaatagtt gccgtggctt tcacgtaata ccgatcacca atcaacacaa cttcatcgct
     8581 gattgataaa aacagaccat tcagtaacgg cttaacgcct tcaagaatgt cttcgcagct
     8641 tctgtattta tatttaccga atgagttgta ctggttcttt ggcgcgttca agttctcctg
     8701 aatagctgcc agtcttgcgt aaaattcttt gctcatatgt ttgttctcag aatgggcatg
     8761 gcccaaggaa ataacgctga tttaatactt cgactcggga caaattaagg catacccgca
     8821 ttccttcgcg gtcgccatta tggcgatacc agagagcttt ctgcgtgtac atgcgtctct
     8881 gtaacttgct ctccttcact gtggttgcaa gtgacatgaa tatctccttc gttaccgatt
     8941 aattctttca tctgacgaat gaattcttcg tctgaccagt tatctgtaaa actcatttcc
     9001 tgcgatacca cggaatattg atagctgatt tcatcgcttt atttgcttca agccacattt
     9061 ttgaatcacc aataaatctg gctattactg ctttgttttg tgctgcacga agcatctggt
     9121 gattgatggc tatttcattg cgcataacgc ctccagttgt ttctttgctg cactgattaa
     9181 ttgtttaact cggcgtgata attcagattc gtgcgggtag aaagcggaca tgacgccgct
     9241 acccgcgagc tgaaagtgca tcatggataa ctccttctgt ttgattgcat aacgaaaacg
     9301 cctcgagtga agcgttattg gtatgcatat aaaaaagccc tcatactgga gggcaaagaa
     9361 gatttcaaat aatcagaaca agtcggctcc tgtttagtta cgagcgacat tgctccgtgt
     9421 attcactcgt tggaatgaat acacagtgct tactcgtact aataaaatac ccaattttct
     9481 gtttcttggt tgtgcccaaa gttatattca atatctggtg ttgatgtatc aatattcttc
     9541 atcccatcaa caagagttga tacaacagcc aaatcctgtt tgattctcat taaatggtat
     9601 ttcttccggc gcaataaact ttcaatggca agtttcttcg ttgggaatgc aaaagatctt
     9661 tctgcatttt ttgctacttt cttaattgca tatctatttc tcttttgttt ccattcctgt
     9721 aaccactgat ttggtgctgg tttaaaatta acaatccaat gcgcaggaac caaccatgca
     9781 taatgctctg tctgatgaaa agctatatat tgaagtgcga atattttgat tccatcttct
     9841 tcaactgtcg cctggaatct ccagaaaaca ggcattccat catgttcagt ttctgattca
     9901 ggaaaaggta cgctccatga ttttgtcata tctcacctca aataagtggc ttgctgccta
     9961 atttcatttt ctggcgacca acacaagtca cacccatttc actgcgtggc ttgctgtacc
    10021 atgtgcgctg attcttgcgc tcaatacgtt gcaggttgct ttcaatctgt tcgtggtatt
    10081 cagccagcac cgtaaggtct atcggattca gtgcgctttc tactcgtgat ttcggtttgc
    10141 gattcagcga gagaataggg cggttaactg gttttgcgct taccccaacc aacaggggat
    10201 ttgctgcttt ccattgagcc tgtttctctg cgcgacgttc gcggcggcgt gtttgtgcat
    10261 ccatcttgat tctcctgtca gttagctttg gtggtgtggt aggtgggaga cccatttcga
    10321 cctgcttcgg ccgacttcaa ttcggcaata gttccgcagg cctcgccgct ttacgtgcga
    10381 catattccca tccatgaacc cttcaccaca ccccaaagcc ttctgctttg aatgctgccc
    10441 ttcttcaggg cttaattttt aagagcctca ccttcatggt ggtcagtgcg tcctgctgat
    10501 ggcttaaaat tacaagaaag attgtatgtt gtaaacaaga aatattgtaa aaaggggcgt
    10561 aaaaacaaac tccattgttt ttaaatgaaa aatagtttgt ttttggttat cgagattgag
    10621 gtggggatta ctggttgcag gttccgacta catcaccaac aaatgatttg gttgatgtaa
    10681 gttgttgcat acctgggata ttcattactt tggagtaaag agcttttttg tctgtagtga
    10741 ttgatcaagt ttcaacggtt attcctcctc cagactggta ttctcctacc atagtgttcg
    10801 atgacaaagc agtgtatttc atctctggat agacgccaga aactgattca taaactgatg
    10861 atttatcgcc atttattgtt acgtggaaaa cggaatcttc cgtgctgtct tttgtaaact
    10921 cgtaacgatc gccattcatt gacccgtacc cgtgcaggtt tgtgacaatc cagcattcag
    10981 aattggcgct ggtagttaag agtattgaga gtagcgccgc aatcctgatc atacgaattt
    11041 taccctcgct tccacgacaa caccgataat cttgcagttc ccgttgatag gagtcatagg
    11101 ccatgaaggg ttcaggcctt tcaggtactt ctgcccgcca tctataacca gtttcttgaa
    11161 tgttgcttcg ttcgcgtcag tcagtttggc tacaacaagg cttccattca ctggctcgcg
    11221 tccagtatct actaacacca tatgaccttc agggatgctt tgacctacag gtgaggtcat
    11281 ggaatcacct tcaaccttca gccagaatcc atcgcctaat aagttaacgt cactgtcata
    11341 ccattcatca atgtccttga tatcgtaggg ttcacaagct tcacaccacg aaccagctct
    11401 aaccatgcta atcaatggat atttcccttt gggctcaaca tgcccaacaa atctaacatt
    11461 cgaatcagag gtgccattga gcagccagtc aacacttacg ccaagagctg acgcaagttc
    11521 tggtaaaaag cgtggtcgct tagttttacc gttttcgagc tgctctatag actgctgggt
    11581 agtccccacc ttttgagcaa gttcagcctg gttaagtcca agctgaattc ttttgctttt
    11641 taccctggaa gaaatactca taagccacct ctgttattta cctccaatct tcacaagaaa
    11701 aactgtattt gacaaacaag atacattgta tgaaaataca agaaagtttg ttgatggagg
    11761 cgatatgcaa actctttctg aacgcctcaa gaagaggcga attgcgttaa aaatgacgca
    11821 aaccgaactg gcaaccaaag ccggtgttaa acagcaatca attcaactga ttgaagctgg
    11881 agtaaccaag cgaccgcgct tcttgtttga gattgctatg gcgcttaact gtgatccggt
    11941 ttggttacag tacggaacta aacgcggtaa agccgcttaa gacattcccg ctcttacaca
    12001 tcccagccct gaaaaagggc attaccagaa acaaatctct atggttttgc gtttctttgc
    12061 gaagccaact ctatctaatc attaaggaaa ttatctatgg gtactattgc aactaaaagc
    12121 aagaaagcgg ctcgcatcga gtcagccttg ctgaacaaac tggcactgat ggggcagaag
    12181 acattcgctc gagcaatggg ggttcctgaa tatcaggtaa gccgatggaa gaatggtttc
    12241 ttctcgcagg taagcatgat gcttgctgtt ctggaatacg gaatcgaaga cgatgaaatg
    12301 gctgaattga ctaagcggct tgccgattac ctgacaaaag aaaaagcccc gaagaacggc
    12361 gaattcttcg aggcctaatg tagaaagact ggatcaatcc acgggagtaa ttatgacaaa
    12421 acgtcgtaag aaataccagg aaaaagaaga gattcgacac cctgattcac ctgagggatt
    12481 agtggtagcc gcagcaaata acagggcgtt cgcagagcgc cttgttggtg tttacagact
    12541 agccaaagca ggagtgaaac atgggcgtcg ttaagttagc tgattacagg cctcaactgg
    12601 aggtcgtgga gcatcgcgtg gcagaactcg aagatggcta cactcggact gcaaacacac
    12661 tgttagaagc cgccatgctt tctggactta ctctacatca gttactgatt gttatggctg
    12721 tgtggcgcaa gacatacggt tacaacaaaa aaatagattg gatcggaaac gaacagttcg
    12781 ctgaactcac tggcatggca ccaaccaaat gctctaccgc caaaaacgag cttatcagaa
    12841 tgggagttct cattcaggtg gggcgtcaag ttggtatgaa tacaaacatt tccgagtgga
    12901 aaacgaaagt taacggattc ggtaaaacat ttaccaattc ggtaaaacaa accttcacca
    12961 aatcggtaaa aagcaattta ccgaatcagt caaacacaaa agacaatata caaaagacaa
    13021 taaatacaaa taccccctta ccccctaacg ggggcggcga tgggcaggtt aaacctgaac
    13081 gtcgcaaggc agaacgaatc gactacgaat ccttcctgaa cgcctacaac accgaagtcg
    13141 gtgacagact gccacacgct gttgcggtca acgagaaacg caaacgccgc ctgaagaaaa
    13201 tcatcccgca actgaaaacg ccaaacgtgg acggtttcag agcgtatgtc agggcgtttg
    13261 tgcatcaggc caagccgttt tacttcggag acaacgacac gggctggacg gccgattttg
    13321 attacctgct gagggaagac tcgttaacgg gggtacggga agggaagttt gcagacaggg
    13381 ggattgcatg aaacaggata tcgaagcgag cgttatcggt ggcctgctga ttggtggatt
    13441 aacaccaacc gccagcgacg ttctggcaac gctggagccg gaagcgtttt caattccgct
    13501 ctaccggaaa gccttcgagg ttatccgcaa gcaggcgcga aacagaaacc taatcgacgc
    13561 gctgatggtt gccgaggcgt gcggagagga gcatttcacg tcaatcctga tgaccagcaa
    13621 gaactgcccg agtgccgcaa acctgaaggg atatgctgga atggtcgcgg ataactatca
    13681 ccgccgtctg gtgctggaaa tcatggatga aatgcgtgaa ccaatccaaa gcggaaccat
    13741 cgacgcatcg agtcaggcga tggatgaact tgtaaagcgt ctttcagcca tcagaaagcc
    13801 ccgtgacgag gtaaaacctg tacggttagg ggaaatcatc actgactaca ctgacacgct
    13861 tgacaggcgt ctgaggaacg gagaagagtc agataccctg aagaccggaa tcgacgaact
    13921 tgacgccatc accggaggga tgaacgcaga agacctggtg attatcgccg ctcgtcctgg
    13981 tatgggtaaa accgaactgg cgctgaagat tgccgaaggc gttgcaagcc gcgttattcc
    14041 tggttctgac gtccggcgcg gagtgttgat tttctcgatg gaaatgagcg cattgcagat
    14101 tgcagagcgg agcattgcca acgccgggag gatgtcggtt agcgtgctgc gaaatcctgc
    14161 atcgatggat gacgaaggct gggcgcgtgt tgctaacggc atgagtcagc ttgcagattt
    14221 ggatgtatgg gtagtcgatg cctcgcggtt atcggtcgaa gaaatacgct caatcgcaga
    14281 acggcacaaa caggaaaatc caaacctgtc actcatcatg gcggattatc ttggcctgat
    14341 tgagaagccg aaagcagacc gcaacgacct cgcaattgct cacatctccg gaagcctgaa
    14401 ggcgatggcg aaagacctga aaacgccagt tatctccctg agtcagcttt cgcgcgatgt
    14461 tgagaagcga ccaaacaaac gcccgacaaa cgcagatttg cgtgattcag gaagcattga
    14521 acaggacgca gactcaatca tcatgctcta tcgggaagcg gtatatgacg agaacagtag
    14581 cgccgcgcca tttgctgaaa tcatcgtgac gaaaaaccgt tttggctcgc ttggtacggt
    14641 ttaccagcgg ttctgtaacg gacactttgt tgcatgtgac caggatgaag ccagacagat
    14701 ttgcacagca tcaaatgcac ctgctgcgcg tggcagacga tatgcacaag gggctgacgt
    14761 atgaccatct acatcactga gataataaca ggcctgctgg taatcgcagg cctttttatt
    14821 tgggggagag ggaagtgtgg ctgactggca aattccaatc atcattcttg ccggagcttc
    14881 gctggttgct ggctttatcc tgctgaaaaa gcataaagac cgtgatcaaa aagttgaagt
    14941 tctctatggg tatccagcga acagcacaac atggctgacc atttaccact accgaaaatc
    15001 aggacgctgg gtattcgaat gggatgatct gttcgctgaa aagcgaccaa agtcatgggg
    15061 agacatcagc gaatgcatga tgtttgaaga aagaaaatcc ggcgcaaccc gagaagagtt
    15121 taacgaagcg tgggcgcgat taagtgagag agggtatttg tgagcaagta cgaaaaatta
    15181 gatcaaaaca ttctttcaat gctgagtgaa agaccaacac ctgtttttga tatctggctt
    15241 aaatggcgga gcaatggaat gtatatcgaa accatcgatc gccgtatgca atacctgaga
    15301 aagaaagggc ttgttgcaaa tgtgcgtggg aagggttggg tgaaaattaa cctgtcataa
    15361 cggggattga tatggacgat agcttagaaa tatttgaaga gtggtgcgct gatgaacttg
    15421 gtgtctccgc ttacttcatt cgacaaatgc gaagcaagaa tattttgggc gtgattgagt
    15481 ataaacgagt tgaaataaac aagagataca gggcttggat ggccgcagtt cgcgccgccg
    15541 gagtcaaagt gaaggagtga gtatgagcgc atacgaagaa atcatgttag ccctgcgatt
    15601 ctttttcggt gtggaagaag atgaaaacgt aaatgagatt atcggacaag accatgaccc
    15661 gatagggact attgcagctg cacttgacga ttacaggagc gtaaatggtg aggaaactaa
    15721 cgtttgaact aagaagcccc atccatcagc agaacgccat tcaagctatc cagcaaatcc
    15781 ttccagaccc aaccaaacca atcgtagtaa ccattcagga acgcaaccgc agcttagacc
    15841 agaatcgaaa gctttgggct tgccttggtg acgtttcgcg tcaggttgaa tggcatggtc
    15901 gctggctgga tgcagaaagc tggaagtgtg tgtttaccgc agcattaaag cagcaggacg
    15961 ttgttcctaa ccttgccggg aatggatttg tggtaatagg ccggtcaacc agcaggatgc
    16021 gtgtaggcga atttgcggag ctattagagc ttatacaggc attcggtaca gaacgtggcg
    16081 ttaaatggtc agacgaagcg cgactggctc tggagtggaa agcgagatgg ggagataaaa
    16141 ctgaatgaac aaataccgac ttatttacgc agatccgcct tggcaatatc gcgacaaagc
    16201 caacgatggc aatcgcggtg ctggacataa atacgatgtt atgaatgttc aggacatttg
    16261 ccgactgcca gtatgggatt tagcggatcc agaatcttgc ttgttagcga tgtggtgggt
    16321 gccgacacag ccagccgaag cgctaaaggt aattgaggcg tggggattca ggttgatgac
    16381 tatgaaaggc tttacttggc acaaaaccaa taagcacaaa ggcaacagtg cgatcggaat
    16441 ggggcatatg accagggcaa atagcgagga ttgcttgttt gccgttcgag ggcggttacc
    16501 tgagagaatg gacgcttcaa tatgccagca ctttaccgca ccgagaatgg agcacagtgc
    16561 aaaaccaccg atcgtaagag acatgttagc taagttgctt ggcgacgtgc cgcgctgtga
    16621 gttatttagc cgcgacaaag tgaccgggtg ggatatgtgg ggaaatcagt gcgactccga
    16681 ttttgaactt gctcccggca tggcgattaa accatgcaaa atggtgatcg catgaagcac
    16741 tgttatcgat gtggagagcg aaaggaagac gatcgctttc gaccaggaca gccttactgg
    16801 aatcgatggt gtctccggtg tgaaagaaca ccaacagggg tgttaccact accgcaggaa
    16861 aaggaggacg tgtggcgaga cagcgacgaa gtatcaccga cataatctgt gaaactgcaa
    16921 ataccttcca acgaaacgct ccagaaataa acgcaagcca atcccaaaag aatctgacgt
    16981 aaaaaccttc aactacacgg ctcacctgtg ggatatccgg tggctaagac atcgtgcgag
    17041 gaaatgacaa tgcttttaat tcaacctgga tttggactta gcatcaaaaa aggccacatg
    17101 tttggcgaga aagagtcaca acgaaaaatg atgtctatcc ggttgccatt tatcagtatt
    17161 tattggctaa acagggaggc aacaaattat tggtatacat gcgccagagc agcatttaac
    17221 gaccctgact ggtttgtgga aaaccaccac gcagttcgtc aggcaaagag aaaggccaac
    17281 atgacataca tgaaggcgta tcgaaaagca tggaaagaac accgcgatcg ataccagcaa
    17341 gatatggaaa agcttgaatc agaaaacatg gaattaagac gaaagcttgg tgaagcaaaa
    17401 cgagacattg atgcttacaa gcgacttttt aatggtgaaa gccatgctta gtccatccca
    17461 atctcttcaa taccagaaag aaagcgtcga gcgggcttta acgtgcgcta actgcggtca
    17521 gaagctgcat gtgctggaag ttcacgtgtg tgagcactgc tgcgcagaac tgatgagcga
    17581 tccgaatagt tcaatgtacg aggaagaaga cgatggccaa accagcgcga agacgatgta
    17641 aaaacgaaga atgtcgggaa tggtttcacc ctgcattcgc taatcagtgg tggtgctctc
    17701 cagagtgtgg aaccaagata gcactcgaac gacgaagtaa agaacgcgaa aaagcggaaa
    17761 aagcagcaga gaagaaacga cgacgagagg agcagaaaca gaaagataaa cttaagattc
    17821 gaaaactcgc cttaaagccc cgcagttact ggattaaaca agcccaacaa gccgtaaacg
    17881 ccttcatcag agaaagagac cgcgacttac catgtatctc gtgcggaacg ctcacgtctg
    17941 ctcagtggga tgccggacat taccggacaa ctgctgcggc acctcaactc cgatttgatg
    18001 aacgcaatat tcacaagcaa tgcgtggtgt gcaaccagca caaaagcgga aatctcgttc
    18061 cgtatcgcgt cgaactgatt aaccgcatcg ggcaggaagc agtagacgaa atcgaatcaa
    18121 accataaccg ccatcgctgg actgtcgtag agtgcaaggc gatcaaggca gagtaccaac
    18181 agaaactcaa agacctgcga aatagcagaa gtgaggccgc atgacgttct cagtaaaaac
    18241 cattccagac atgctcgttg aagcatacgg aaaccagaca gaagtagcac gcagactgaa
    18301 atgtagtcgc ggtacggtca gaaaatacgt tgatgataaa gacgggaaaa tgcacgccat
    18361 cgtcaacgac gttctcatgg ttcatcgcgg atggagtgaa agagatgcgc tattacgaaa
    18421 aaattgatgg cagcaaatac cgaaatattt gggtagttgg cgatctgcac ggatgctaca
    18481 cgaacctgat gaaaaaactg gagacgatag gattcgacac caaaaaagac ctgcttatct
    18541 cggttggcga tttggtcgat cgcggtacag agaacgtcga atgcctggaa ttaatcacat
    18601 tcccctggtt cagagctgta cgtggaaacc atgagcaaat gatgattgat ggcttatcag
    18661 agcgtggaaa cgtcaatcac tggctactta atggcggcgg ctggttcttt aatatcgatt
    18721 acgacaaaga aattctggct aaagctcttt cccataaagc agatgaactt ccgttaatca
    18781 tcgaactggt gagcaaagat aaaaaatatg tcatctgcca cgccgattat ccttgtgacg
    18841 aatacgagtt tggaaagcca gttgatcatc agcaggtaat ctggaaccgc gaacgaatca
    18901 gcaactcaca agacgggatc gtgaaagaaa tcaaaggcgc ggacacgttc atctttggtc
    18961 atacgccagc agtgaaacca ctcaaatttg ccaaccagat gtatatcgat accggcgcag
    19021 tgttctgcgg aaacctcaca ttgattcagg tacagggaga aggcgcatga gactcgaaag
    19081 cgtagctaaa tttcattcgc caaaaagccc gatgatgagt gactcaccac gggccacggc
    19141 ttctgactct ctttccggta ctgatgtgat ggctgctatg gggatggcgc aatcacaagc
    19201 cggattcgga atggctgcat tctgcggtaa gcatgaactc agccagaacg acaaacaaaa
    19261 ggctatcaac tatctgatgc aatttgcaca caaggtatcg gggaaatacc gtggtgtggc
    19321 aaagctcgaa ggaaatacta aggcaaaggt actgcaagtg ctcgcaacat tcgcttatgc
    19381 ggattattgc cgtagtgccg cgacgcctgg cgcaagatgc agagattgcc acggtacagg
    19441 tcgtgcggtt gatatagcca aaacagagca gtgggggaga gttgtcgaga aagagtgcgg
    19501 aagatgcaaa ggcgtcggct attcaaggat gccagcaagc gcagcatatc gcgctgtaac
    19561 gatgctaatc ccaaacctta cccaacccac ctggtcacgc actgttaagc cgctgtatga
    19621 cgctctggtg gtgcaatgcc acaaggaaga gtcaatcgca gacaacattt tgaatgcggt
    19681 cacacgttag cagcatgatt gccacggatg gcaacatatt aacggcatga tattgactta
    19741 ttgaataaaa ttgggtaaat ttgactcaac gatggataaa tgcactcgtt aaataaagcc
    19801 ctgagttaat agctcggggc tttttgcgtt ttaagcacgg cctttctgaa agcacatcaa
    19861 gccaaatacc agacagacaa aaataatcac cttatccgct tggctacggt gcggtgtgct
    19921 ttgcataaaa gaaaaccagc gcaatggctg gcttcgtgaa agcgggtggc aagaggctgc
    19981 gctaacaacc tcatgccgtt ttgcccgtgc atatcggtca cgaacaaatc tgattactaa
    20041 acacagtagc ctggatttgt tctatcagta atcgacctta ttcctaatta aatagagcaa
    20101 atccccttat tgggggtaag acatgaagat gccagaaaaa catgacctgt tagccgccat
    20161 tctcgcggca aaggaacaag gcatcggggc aatccttgcg tttgcaatgg cgtaccttcg
    20221 cggcagatat aatggcggtg cgtttacaaa aacagtaatc gacgcaacga tgtgcgccat
    20281 tatcgcctgg ttcattcgtg accttctcga cttcgccgga ctaagtagca atctcgctta
    20341 tataacgagc gtgttcatcg gctacatcgg tactgactcg attggttcgc ttatcaaacg
    20401 cttcgctgct aaaaaagccg gagtagaaga tggtggaaat caataatcaa cgtaaggcgt
    20461 tcctcgatat gctggcgtgg tcagagggaa cagataacgg acgacagaaa accagaaatc
    20521 atggttatga cgtcattgta ggcggagagc tatttactga ttactccgat caccctcgca
    20581 aacttgtcac gctaaaccca aaactcaaat caacagcagc cgggcgctat cagcttcttt
    20641 cccgttggtg ggatgcctac cgtaagcagc ttggcctgaa agacttctct ccgaaaagcc
    20701 aggacgctgt ggcattgcaa cagattaaag agcgtggcgc tttgccgatg attgatcgcg
    20761 gtgatatccg tcaggcaatc gaccgttgca gcaatatctg ggcttcgttg cccggggctg
    20821 gttatggcca gttcgagcat aaggctgaca gcctgattgc aaaattcaaa gaagcaggcg
    20881 gaacggtcag agagattgag gtatgagcag agtaaccgcg attatctccg ctctggttat
    20941 ctgcatcatc gtctgcctgt catgggctgt taatcattac cgtgataacg ccatcgccta
    21001 caaagagcag cgcgacaaag ccgcatccac tatcgctgat atgcagaagc gtcaacgtga
    21061 cgtagcagaa ctcgacgcca gatatacaaa ggagcttgct gatgctaacg cgactatcga
    21121 aagcctccgt gctgatgttt ctgctggtcg taagcgcctg caagtcgccg ccacctgtgc
    21181 aaagtcaacg accggagcca gcagcatggg cgatggagaa agcccaagac ttacagcaga
    21241 tgctgaactc aattattacc gtctccgaag cggaatcgac aggataaccg cgcaggttaa
    21301 ctacctgcag gaatacatca ggactcagtg cctgaaataa tttttttgca aatcacaaag
    21361 tccatttaat gagcctcgcg atgcggggct tttttacatc tgaatttcac agcgcatctc
    21421 acgcgcatat tacatcaccc gagcctttca gaaagttgag cctgagaact gccgtatatg
    21481 gtggcgacca tctcgggacg gcttttctgt gagacaggct cactttctaa aaggtaaaga
    21541 cgcaatgaac tacccaaccg ttgttaacga tatagatttc agagacctaa tttttgtagc
    21601 aaacaacgat ccggttacag attcttttat ggtggcaaaa gcatttggaa agctgccgaa
    21661 gaacgtagtt cgtgacattg agcgaaccat agaagcttgc cctcctgagt ttgatacaaa
    21721 gctcaacttt gagctttgct ataaaaacaa tgagttacag aatggtaagc cgcaaaaatt
    21781 ctaccgtctc cgcaaggatg ggttgatgct tttggttatg tccgacacca aaaaagaagc
    21841 aatgcgtatc aaaattgctt acatcaacgc atttaactgg atgtacgcca tgcttcaggt
    21901 tggtcatcgt caatttgaag aagagagaaa tgccgtaatg ctggagtaca tgaaagagaa
    21961 ggatgttgcc agcatgtcag gtcgcctgct aaatcgctgg ggcaaaatta agaagccaca
    22021 gctgatggct agaattgaac gccttgaaca gcacgggcaa accgtaatcc ccggactcat
    22081 caattaacgg cagcaccgcg aaacaaccca agccagtaag tggggaaata acactggcag
    22141 ccactgaaag atgaacctcc agccttatgg caaaaaagat tctttgtggt ggcggactga
    22201 tggaaagaca tcggatagaa tcaagcagtg gctagggtag ctcccgaaaa gcggactcgt
    22261 caccgcctgc cactgaatct atggcgaaca actagacgag gttgtgatgg atgataacgg
    22321 aaaccaatgg gtcaatgttg aacaccggct accagaaagc aaagaaggga tgtggtctaa
    22381 agaggttata gctcttactg atactggtga cgtgttcaaa ctatcatgta tgggctctta
    22441 ctggcagaga accaaggcat tcatcgattc aggagcaagc aaggttacac actggatgcc
    22501 gcttaattac ccagatgatt aaaacggatg aaaaatgaaa ggtcgctcag gcggcctttt
    22561 ttattgccat cacaaaagcc attccttact gagtggcttt gataatggct tataccctac
    22621 acgggataac ttaactgata tcccttttaa cggataaatg gagccaacaa tggcagagat
    22681 tattcccatg actgaagaac agaaattcca gttagagatt tacaaactgg tcatgaacca
    22741 gaacgcagcc gcagaagaag catttcaatt cattggcact gacgaactga agcttgagct
    22801 attcaaaatt cacttccagt caggtggcgc taattcagat atcacgatcc gcacatttga
    22861 agcggtgcgt aaatcgaagg aagcgttaga cctgttcacc accggagcat gatgctcaac
    22921 ctgaaataac gattaagtga gatgaatatg gcagcaccaa agggcaaccg attctgggag
    22981 gcccgcagta gtcatgggcg aaaccctaaa ttcgaatcgc ctgaggcgct gtgggctgct
    23041 tgttgtgaat acttcgagtg ggctgatgat aacccgctat gggagggtaa ggtattttca
    23101 tatcagggag aaataattaa ggctaatgtc cctaagatgc gagccatgac tatttcagga
    23161 ttgtgtacct tccttgatat caccaggcaa acatggggaa ccttccggtc aatggaaggt
    23221 ttttctgacg tcacatcacg agcggaagac atcatctacg accagaaatt ctctggcgca
    23281 gccgctgacc ttctcaacgc taacatcatc gcccgtgatt tgggcctcaa agagcagtcg
    23341 caagttgaag acgtgacacc tgataaggga gatcgcgata agcggcgctc tcgtatcaag
    23401 gagctattca accgtggaac tggacgcgat tcttgataac ttgagcgacg aagagcaaat
    23461 cgaattgctc gagctactcg aagaagaaga gaactaccgt aacacacacc tgctatatga
    23521 atttacgcca tacagcaaac agcgtgagtt catcgacgcc gggcatgact atccagagcg
    23581 ctgttttatg gctggtaacc agcttggtaa gtcatttact ggtgctgctg aagtcgcgtt
    23641 tcaccttacc gggcgttatc cgggcacaaa aggctatcct gctgatggta aatatggcgg
    23701 tgagtggaaa ggtaagcgtt tttatgagcc tgttgtcttt tggattggtg gcgagacaaa
    23761 cgagactgta accaaaacga ctcaacgcat cctgtgtggt cgtatcgaag agaatggtga
    23821 gccaggctac ggttccatac ctaaagaaga catcattagc tggaagaagt ctcctttctt
    23881 tccgaacctt gttgatcacc ttctggttaa gcatcacacg gctgatggcg ttgaagatgg
    23941 catttcaatc tgctacttca agccatactc gcaaggtcgt gctcgctggc agggtgacac
    24001 aatccacggc gtgtggtttg acgaagagcc accatacagc atttatggtg aaggccttac
    24061 ccgtaccaac aaatacgggc aattctcaat tctgacgttt accccgctga tggggatgtc
    24121 tgacgttgtt accaagttcc tgaagaatcc aagcaagtcg cagaaagtgg tcaacatgac
    24181 catctacgac gctgagcact acacagacga acagaaagag caaatcatcg catcttatcc
    24241 tgagcatgag agagaggcgc gtgctcgcgg tattcctacg atgggtagtg gtcgaatctt
    24301 ccagataccg gaagagacga ttaagtgtca gccgttcgag tgtcctgatc acttctacgt
    24361 aattggcggg atggatttcg gatgggatca cccgcaggcg caggttcagc tttggtggga
    24421 caaggacgca gacacaatat acgtttcacg cgtgtggaag gcgaaagaaa aaacagccgt
    24481 tcaggcgtgg ggagccgtta aatcatgggc gcataaagtg ccaactgcat ggcctcatga
    24541 cggaaaccag cacgagaagg gcggcggtga gcagctcaaa gggcagtatg cagatgctgg
    24601 ttttatgatg ttgcaggagc atgcgacatg gcctgatggc ggtaatgctg tggagcctgg
    24661 catcactgaa ttgcgcgaca tgatgctcga tggtcgcttc aaagtattca acacctgtga
    24721 gccattcttt gaggagttcc gcctctatca ccgtgatgaa aacgggaaaa tcgtcaagct
    24781 taacgacgac gttctctcag ccgttcgcta tgcatacatg atgcgccgct tcgccaaaat
    24841 gatgcgcgac atcaaaaaac caaaagagaa aaagatacca gccccaatca ggcccatcgc
    24901 acggagaact taaatggccg acgaaaacag actcaattcc attctgtgta agtttgacgc
//
LOCUS       DAZSIO010000045        24591 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZSIO010000045 DAZSIO010000000
VERSION     DAZSIO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.045"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(14..106)
                     /locus_tag="ACHX5T_001694"
     tRNA            complement(14..106)
                     /locus_tag="ACHX5T_001694"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(70..72),aa:Ser,seq:gct)
     gene            complement(422..607)
                     /gene="csrA"
                     /locus_tag="ACHX5T_001695"
     CDS             complement(422..607)
                     /gene="csrA"
                     /locus_tag="ACHX5T_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR8466932.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(842..3472)
                     /gene="alaS"
                     /locus_tag="ACHX5T_001696"
     CDS             complement(842..3472)
                     /gene="alaS"
                     /locus_tag="ACHX5T_001696"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR8466933.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3600..4100)
                     /gene="recX"
                     /locus_tag="ACHX5T_001697"
     CDS             complement(3600..4100)
                     /gene="recX"
                     /locus_tag="ACHX5T_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR8466934.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4343..5404)
                     /gene="recA"
                     /locus_tag="ACHX5T_001698"
     CDS             complement(4343..5404)
                     /gene="recA"
                     /locus_tag="ACHX5T_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR8466935.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5484..5981)
                     /gene="pncC"
                     /locus_tag="ACHX5T_001699"
     CDS             complement(5484..5981)
                     /gene="pncC"
                     /locus_tag="ACHX5T_001699"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR8466936.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6126..7211)
                     /gene="mltB"
                     /locus_tag="ACHX5T_001700"
     CDS             complement(6126..7211)
                     /gene="mltB"
                     /locus_tag="ACHX5T_001700"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR8466937.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7467..8029
                     /gene="srlA"
                     /locus_tag="ACHX5T_001701"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7467..8029
                     /gene="srlA"
                     /locus_tag="ACHX5T_001701"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: protein motif:HMM:NF015565.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8026..8985
                     /gene="srlE"
                     /locus_tag="ACHX5T_001702"
                     /gene_synonym="gutE"
     CDS             8026..8985
                     /gene="srlE"
                     /locus_tag="ACHX5T_001702"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR8466938.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8996..9367
                     /gene="srlB"
                     /locus_tag="ACHX5T_001703"
                     /gene_synonym="gutB"
     CDS             8996..9367
                     /gene="srlB"
                     /locus_tag="ACHX5T_001703"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR8466939.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9371..10150
                     /gene="srlD"
                     /locus_tag="ACHX5T_001704"
     CDS             9371..10150
                     /gene="srlD"
                     /locus_tag="ACHX5T_001704"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFR8466940.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10255..10614
                     /gene="gutM"
                     /locus_tag="ACHX5T_001705"
     CDS             10255..10614
                     /gene="gutM"
                     /locus_tag="ACHX5T_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR8466941.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10681..11454
                     /gene="srlR"
                     /locus_tag="ACHX5T_001706"
                     /gene_synonym="gutR"
     CDS             10681..11454
                     /gene="srlR"
                     /locus_tag="ACHX5T_001706"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR8466942.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11447..12412
                     /gene="gutQ"
                     /locus_tag="ACHX5T_001707"
     CDS             11447..12412
                     /gene="gutQ"
                     /locus_tag="ACHX5T_001707"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR8466943.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12409..13923)
                     /gene="norR"
                     /locus_tag="ACHX5T_001708"
     CDS             complement(12409..13923)
                     /gene="norR"
                     /locus_tag="ACHX5T_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR8466944.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14110..15549
                     /gene="norV"
                     /locus_tag="ACHX5T_001709"
     CDS             14110..15549
                     /gene="norV"
                     /locus_tag="ACHX5T_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR8466945.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15546..16679
                     /gene="norW"
                     /locus_tag="ACHX5T_001710"
     CDS             15546..16679
                     /gene="norW"
                     /locus_tag="ACHX5T_001710"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR8466946.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16807..19059)
                     /gene="hypF"
                     /locus_tag="ACHX5T_001711"
     CDS             complement(16807..19059)
                     /gene="hypF"
                     /locus_tag="ACHX5T_001711"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR8466947.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19212..19739)
                     /gene="hydN"
                     /locus_tag="ACHX5T_001712"
     CDS             complement(19212..19739)
                     /gene="hydN"
                     /locus_tag="ACHX5T_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR8466948.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19888..20898)
                     /gene="ascG"
                     /locus_tag="ACHX5T_001713"
     CDS             complement(19888..20898)
                     /gene="ascG"
                     /locus_tag="ACHX5T_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR8466949.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20960..21031)
                     /locus_tag="ACHX5T_001714"
                     /pseudo
     CDS             complement(20960..21031)
                     /locus_tag="ACHX5T_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21041..22750)
                     /gene="hycE"
                     /locus_tag="ACHX5T_001715"
     CDS             complement(21041..22750)
                     /gene="hycE"
                     /locus_tag="ACHX5T_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR8466950.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22768..23691)
                     /gene="hycD"
                     /locus_tag="ACHX5T_001716"
     CDS             complement(22768..23691)
                     /gene="hycD"
                     /locus_tag="ACHX5T_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR8466951.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23694..>24591)
                     /locus_tag="ACHX5T_001717"
     CDS             complement(23694..>24591)
                     /locus_tag="ACHX5T_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HFR8466952.1"
                     /translation="ALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGSVWFRTGHRD
                     IEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVARLL
                     GPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVALAICCV
                     IGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKGDRLPS
                     RSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEGSAL
                     LFRRMALVELAVLVVIIVSRGA"
BASE COUNT         5800 a         6918 c         6308 g         5565 t
ORIGIN      
        1 ctacggatgc aaatggcggt gaggcgggga ttcgaacccc ggatgcagct tttgaccgca
       61 tactccctta gcaggggagc gccttcagcc tctcggccac ctcaccacac gcctcttacg
      121 agtgcttcga agaacttgtt tatcgctcat catcgctgcg tggcgcacat attactttct
      181 gagacttata agtcaaacaa tttttcccac acttttatcg tttgcacact tcacgctcaa
      241 ttagtctgta aaagcgtcaa aaagagtgtt ttatctacag aaaaacggag gtctggcaga
      301 tagcagtaat gcaaaaaaat ggagacttaa gttgaatgaa cgggagtaaa gcgaaaagac
      361 tatagagtga aggagaaatt ttgagggtgc gtctcaccga taaagatgag acgcggaaag
      421 attagtaact ggactgctgg gatttttcag cctggatacg ctggtagatc tcttcacggt
      481 gaacagaaac ttccttcggg gcatttacgc caatacgtac ctggttgccc tttaccccta
      541 aaactgtcac ggtgacctca tccccaatca tgagggtctc accaactcga cgagtcagaa
      601 tcagcattct ttgctccttg aaagattaaa agagtcgggt ctctctgtat cccggcatta
      661 tccatcatat aacgccaaaa agtaagcaat gacaaacaca ttacatgtaa gcagtcatgg
      721 cattacattc tgttaaacct aagtttagcc gatatacaca acttcaacct gactttatcg
      781 ttgtcgatag cgttaatgcg aatgccgtga agcgagtcca cggcattgcc tgacgcttat
      841 attattgcaa tttcgcgctg acccagcctt tcacactggc taacgctgca ggtaaggccg
      901 cagcatccgt accaccggct tgcgccatgt caggacgtcc accacccttg ccgcccacct
      961 gctgagcgac cataccaatc agttcccctg ctttcacacg atctgtgacg tccttagata
     1021 cgcctgcaat cagagaaacc ttaccttcgg ctaccgttgc cagcacgata attgtcgacc
     1081 ccagctgatt ttttaaatcg tcaaccatgg tacgcaacat tttcggctca acaccgctaa
     1141 gctcgctaac caacagctta acaccattaa catcaattgc cttactggaa agatttgcgc
     1201 tctcctgtgc ggcagcttgt tctttaagct gttgtagttc tttttctaac tgacgcgtac
     1261 gttccagtac tgagcgcact ttatccgcca gattattgct atcgcctttc agcagatgcg
     1321 cgacttcgct taagcgatcg ctgtctgcat gaacggtggt gatagcgcct tctccggtta
     1381 ccgcttcgat acgacgaacg cctgcagcag tacccgattc agagatgatg cggaacagac
     1441 caatatcacc agtgcggctg gcgtgagtac cgccacacag ctcggtggag aaatcgccca
     1501 tgctcagcac gcgtacacgc tcatcatact tctcgccgaa cagcgccatc gcacctttcg
     1561 ctttcgccgc ttcgagattc atgatgttgg tttcgatcgg caaattgcga cgaatctgtg
     1621 cgttcaccag gtcttcgacc gcacgaatct cttccggttt catcgcttcg ttgtgtgaga
     1681 agtcgaagcg cagcaccttg tcgttaacca gtgaaccttt ctgcgataca tgagtcccca
     1741 gaacctggcg cagcgcagcg tgcatcaggt gcgttgcgga gtgattcaga cgaatacggg
     1801 cgcgacgagc ctcatcaata tcagcctgca cagcgtcgcc cactttcaga gaacccgcag
     1861 caagtttacc gatgtgacca atcgcctggc cgtatttctg cgtatcttcc accgcgaagg
     1921 agaagttagc gcctttcagt tcgcctttat caccaacctg accgccggat tccgcataga
     1981 atggcgtttg atccagcacg accacagctt cctggccggc attgatggca tcaaccgctt
     2041 taccatcaac aaacagcgcg gtcactttgc cgttcagttc cagatggtca tagcctttaa
     2101 attcagatgc actatcaaca cggatcattg cgttgtaatc ggcaccaaag ccgctggctt
     2161 cgcgcgcgcg acgacgctgc tcttccattg ctgcttcaaa accagcttcg tcaactttga
     2221 tgttgcgctc acgacaaaca tcagccgtca ggtcaaccgg gaagccatag gtgtcgtaca
     2281 gacggaaagc cgtttcacca tccagcgtat caccagaaag ttttgccagc tcttcatcca
     2341 gcaacgccag accgcgctcc agagtacggg caaactgctc ttcttcagtc ttcagcacct
     2401 gctcaacctg cgcctgctgg cgtttcaggt cttcacccgc agagcccata acgtcgatca
     2461 gcggaccaac cagtttgtag aagaaggttt ctttcgcgcc gagcatattg ccgtgacgca
     2521 ctgcgcgacg aatgatacga cgcagtacat aaccacggtt ttcattggac ggcattacgc
     2581 catccgcgat caggaacgca caagaacgaa tgtggtcagc gattacgcgc agcgatttat
     2641 tgctcagatc ggtcgcgcca gtgactttcg ctaccgcctg gatcaacgtg cggaacaggt
     2701 cgatgtcata gttagagtta acgtgttgca gcaccgcagc aatacgctcc agacccatac
     2761 cggtatctac agacggcttc ggcagcggtt ccatcgtgcc atctgcctgg cggttgaact
     2821 gcatgaagac gatgttccag atctcaatgt agcggtcgcc gtcttcttcc ggacttcccg
     2881 gagggccacc ccaaatgtgg tcgccgtgat cgtagaagat ttcggtgcac gggccgcacg
     2941 gaccagtgtc acccatctgc cagaagttgt cagatgcgta tggcgcacct ttgttatcgc
     3001 cgatgcgaat aatacgttcg cgcgggatcc cgacttcttt ttcccagatt tcgtaggctt
     3061 cgtcgtcgct ttcatagacg gtaacccaca gacgctcttt cggcagggca aaccattttt
     3121 cgctggtcag cagttcccat gcaaactgaa tggcatcgtg tttgaaatag tcgccgaagc
     3181 tgaagttgcc cagcatttcg aagaaggtat ggtgacgcgc ggtgtaaccg acgttttcca
     3241 ggtcgttgtg tttaccaccc gcacgcacgc agcgttggga agtggtagcg cgggaataat
     3301 tacgcttgtc gagcccaagg aacacatcct tgaactggtt catcccggcg ttggtaaaca
     3361 acaaagttgg gtcgttatgg ggtaccaggg agctgctggc aactacctga tgtcccttac
     3421 tatggaaaaa gtcgagaaac gcctgacgga tctcagcggt gctcttgctc ataattatcc
     3481 tgaaatcaag ctaacgaaat atcgccacca gctccagcgt gtcttaaccg ccgggctggt
     3541 aactgaaaag tgggaataag ataagttttc ttgactggga agtaaaatac cgtatgcgtt
     3601 cagtcggcaa aatttcgcca aatatcctgg atatcttcca tcagatagcc acgatagagc
     3661 agaaaacgct ggatcttaac tttttctgaa aagacagttg gcaaaggttc accatatttt
     3721 cgcgtcgcct gatcgcgcgc cagtgcgcac cagtcgatgt cacattcacg catcgctttt
     3781 tctgtcgctt cgcgggaaat acctttctga ttcagttcct gacgaatacg tgcaggtcca
     3841 taccctttgc ggctacggct ggcgataaag cgcgcaacaa atcggctgtc atcgagatag
     3901 ccatgttcat ggcaccaggc aataacgcgc tcgtaatctt ctgccgtagc atcaatctct
     3961 tctgggccat ttttgcccat aatcggtgcc gcgagtttac gtcgcagttc ttgctcactg
     4021 tgatcgcgca ccgccagaat gcgtaccgca cgatccaaca ggcgagcata tgccgggcga
     4081 cgggatgttg attctgtcat ggcatatcct tacaacttaa aaaagcaaaa gggccgcaga
     4141 tgcgccccag gttttgctga taaacagatt taatagtcaa tgagtatgca gaatgttccc
     4201 gcaggcccaa ttcgttagag gatcgaggtg agagtaattt ttcacgccag tgctggcctg
     4261 ttcccctcac cctaaccctc tccccaaagg ggcgagggga ccgtccgtgc acacattatc
     4321 tattatcatc aataacgaac gattaaaaat cttcgttagt ttctgctact ccttcgctgt
     4381 catctacaga gaaatccggc gttgagttcg ggttgctcag cagcaactca cgtactttct
     4441 tctcgatctc tttcgcggtt tccgggttat ctttcagcca ggcagttgca ttcgctttac
     4501 cctgaccgat cttctcacct ttgtagctgt accacgcgcc tgctttctcg atcagcttct
     4561 cttttacgcc caggtcaacc agttcgccgt agaagttgat accttcgccg tagaggatct
     4621 gaaattcagc ctgtttaaac ggtgcagcga ttttgttctt caccactttc acgcgggttt
     4681 cgctacccac cacgttttcg ccctctttca ccgcgccgat acgacggatg tcgagacgaa
     4741 cagaggcgta gaatttcagc gcgttaccac cggtagtggt ttccgggtta ccgaacatca
     4801 caccaatttt catacggatc tggttgatga agatcagcag cgtgttggac tgcttcaggt
     4861 tacccgccag cttacgcatc gcctggctca tcatacgtgc cgcaaggccc atgtgagagt
     4921 cgccgatttc gccttcgatt tccgctttcg gcgtcagtgc cgccacggag tcaacgacga
     4981 taacgtctac tgcgccagaa cgcgccaggg cgtcacagat ttccagtgcc tgctcgccgg
     5041 tatccggctg ggagcacagc aggttatcga tatcgacgcc cagtttacgt gcgtagattg
     5101 ggtccagcgc gtgttcagca tcgataaacg cacaggtttt accttcacgc tgcgctgcgg
     5161 cgatcacctg cagcgtcagc gtggttttac cggaagattc cggtccgtag atttcgacga
     5221 tacggcccat cggcagacca cctgccccaa gcgcgatatc cagtgaaagc gaaccggtag
     5281 agatggtttc cacatccatg gaacggtctt cacccaggcg catgatggag cctttaccaa
     5341 attgtttctc aatctggccc agtgctgccg ccaacgcttt ctgtttgttt tcgtcgatag
     5401 ccatttttac tcctgtcatg ccgggtaata ccggatagtc aatatgttct gttgaaacaa
     5461 ttatactgta tgctcataca gtatcaagtg ttttgtagaa attgttgcca caaggtctgc
     5521 aatgcatacg cagtagcctg acgacgcacc gcatcacggt cgccgctgaa gcattcccgc
     5581 cgggtaatgc cttcaccgcg ggcagtggca aaagcaaacc agacggtgcc gacaggcttc
     5641 tcttcactgc cgccatccgg cccggcgata ccactaatag acacggcata atcagcacga
     5701 gccgctttca gtgcacctat cgccatttcc accacgacgg gttcactcac cgcgccatgc
     5761 tgcgccagcg tctcttcgcg tacgccgatc atctgcgctt tggcttcgtt actgtaggtg
     5821 acaaatccgc gttcaaacca ggcggagcta ccggcaatat cggtaatcac tttcgctacc
     5881 caaccaccgg tacaagactc ggcagttgtt acggttgcgc cacgggcttt cagcgcctgc
     5941 ccaacctgtt cgcttaactg catcagttca ctgtcagtca tcacaacctc tgtcagtcaa
     6001 aaatttatgc cggacaagat agcactttcg tcaggaagat ggtgttggcg ttgcaatttt
     6061 acgaggagga ttcagaaaaa agctgattag ccagagggaa gctcacgccc ccctcttgta
     6121 aatagctact gtactcgcgc cagcgccacg gcttgtccta actgccagac cgccattgcg
     6181 taatgggtgc tgtggttgta acgggtgatg gtgtagaagt tcggcagacc gtaccagtac
     6241 tggtagccgg tgccaacatc cagacgcagc aggctggctt gttgatggtt gcccagcggc
     6301 tgctgtggcg ttaaacctgc ggcggcaagc tgcgaaatgc tgtacttagt tttgaaacca
     6361 tttggcaagc ctggagcctg accgtttgcc attatcgcga cctgatcgcc tttcacccag
     6421 ccgtgcgctt tgaaatagtt cgccacgcta ccgatcgcat caaccggatc ccacaggttg
     6481 atatgcccgt cgccgctgaa atctaccgca tattgtttgt aagacgacgg cataaactgt
     6541 ccgtagccca tcgccccggc aaaggaacct ttcagattga gcggatcgtc ctgctcgtcg
     6601 cgcgccatca gcaggaaggt ttccagctcg ccagagaaat actccgcgcg gcgtgggtag
     6661 ttaaatgaca gcgttgccag cgcatcgagg atgcgagttt tccccatcac gcgcccccag
     6721 cgggtttcaa cgccgataat cccgacgata atttccggcg gtacgccata cacctgccac
     6781 gcgcgattca acgcatcttc atactgattc cagaaaacca caccgttctg cacgttgtcc
     6841 ggcgtaataa attttttgcg ataacgcagc catgcgccgt tcggacctga tggcggtttc
     6901 accgatgtgg ttggtgcctg gttatccatc agccgcagta ccgaatccag acgcttcgcc
     6961 tgggagagaa tttcctgcaa ctgctgacga tcgaaaccgt gtttgttcac cattttgtcg
     7021 atgaactgct gggcattcgg gttattagcg aaatcgccgc ccatctgcat cacattgtgc
     7081 tgcggctcaa gcaggaagcc gccagacggc gttccggtgg tcgtttcagt ctcagtaggt
     7141 tttggcttgc tgctacaggc ggcaagcaac acaaaaaggg gaagcaatgt tacataacga
     7201 cgcttgaaca tgaggggtcc atttaacaga ttcaaccagg ggcaagtatg gtaaagcatc
     7261 acaccccgca caaggaagcg gtagtcactg cccgatacgg actttacata actcaactca
     7321 ttcccctcgc tatactttta ttcaaacttt caaattaaaa tatttatctt tcattttacg
     7381 atcaaaataa cacttttaaa cctttcaacc tgattagatt aggttgccgt ttggtaataa
     7441 aacaataaat cctgaaggag agaacaatga tagaaaccat tactcatggt gcagagtggt
     7501 ttatcgggct gttccaaaag ggcggagagg tgtttaccgg gatggtgacc ggcattcttc
     7561 cgctgttgat tagcctgcag gttatcatga acgcactgat taattttatc ggtcagcatc
     7621 gtattgaacg ttttgctcaa cgttgcgccg gtaaccctgt ttcccgttac ctactgttac
     7681 cgtgcattgg cacgtttgtc ttttgcaatc cgatgacctt aagcctgggt cgctttatgc
     7741 cggaaaagta caaacccagc tactacgcgg cggcctctta tagctgccac tcaatgaatg
     7801 gcctcttccc ccatatcaac cctgcgaact gtttgtttat cttggcattg ccagcggtct
     7861 gacaacgctg aacctgccac ttggcccact ggcggtgagt tatctgctgg ttggtctggt
     7921 caccaatttc ttccgcggct gggtgaccga tctgaccacc gccatttttg agaaaaagat
     7981 gggcattcaa cttgaacaaa aagttcacct ggcaggagca acatcatgac gcgtattcgg
     8041 atcgaaaaag gaacgggtgg ctggggcggc ccgcttgagc tggaagccac gccgggcaaa
     8101 aaaatcgtct atatcaccgc cggtacccgg cctgcgattg ttgacaaact ggcacaactt
     8161 actggctggc aggctattga cggatttaaa gaaggtgaac ccgcggaggc ggaaattggt
     8221 gtcgcggtaa tcgactgtgg cggcacatta cgctgcggca tctatccgaa acgacgtatt
     8281 cccaccatta atatccactc gacgggcaag tccggtccgc tggcgcagta cattgtggaa
     8341 gatatttatg tctctggcgt aaaagaagaa aacatcactg tagtgggtga tgcgacacca
     8401 caaccctctt ccgtgggccg tgactatgac accagtaaga aaatcaccga acaaagcgat
     8461 ggtttactgg cgaaggtggg aatgggcatg gggtccgccg ttgccgtgtt gtttcagtct
     8521 ggtcgtgaca ccatcgacac tgtattaaaa accattctgc cgtttatggc attcgtctcg
     8581 gcgctcattg gcatcattat ggcttctggc cttggtgact ggattgccca cggtcttgct
     8641 ccgctagcga gccatccact gggtctggtc atgctggcgc tcatctgctc cttcccactg
     8701 ctttcacctt tcctcggccc aggcgcagtt atcgcacagg ttatcggcgt attgattggc
     8761 gtgcagattg gtctcggcaa tattccgccg catctggctt taccggcact gtttgccatc
     8821 aacgcgcagg cggcttgcga cttcatcccg gtcggtttgt cgctggcgga agcccgtcag
     8881 gacacggttc gcgtcggtgt cccttctgta ctggtgagcc gctttttaac cggcgcgccg
     8941 actgtactga tcgcctggtt tgtctccggt tttatctatc aatagaggct gaaacatgac
     9001 cgttatttat cagaccacca tcacccgtat cggcgcgagt gccactgacg ccctcagcga
     9061 ccagatgctc atcacctttc gtgaaggcgc gccagcggac ctcgaagagt attgcttcat
     9121 tcattgccac ggcgagttga aaggtgcact ccatcccggt ttgcaatttt cactcgggca
     9181 gcatcgctat ccggtgaccg ctgttggcag cgtggcggaa gacaaccttc gcgaactggg
     9241 tcatgtcacc ctgcgcttcg atggtttaaa cgaagcggaa tttccgggca ctgtccatgt
     9301 ggcaggccct gtacccgacg atatcgcgcc gggatcggtt ttgaagtttg aatctgttaa
     9361 ggagtaaaaa atgaatcagg ttgccgttgt catcggtggt gggcaaacct taggcgcgtt
     9421 cctgtgccac ggtctggctg ccgaggggta tcgcgtcgcg gttgtcgata ttcagagcga
     9481 caaagccgca aatgtggcac aagaaattaa cgccgaatat ggtgaaggta tggcgtacgg
     9541 ttttggtgct gacgccacta gcgagcaaag cgttctggcg ctctctcgtg gggtagatga
     9601 aatctttggt cgcgtggatt tgctggtcta tagcgccgga atagcaaaag cagcctttat
     9661 cagcgacttc cagctcggcg attttgaccg ttcgctacaa gtgaatctgg tgggttattt
     9721 cctgtgtgcc cgtgaatttt cgcgtttgat gatccgcgac gggattcagg gtcgcattat
     9781 tcagatcaac tcgaaatccg gcaaagtggg cagcaaacac aactctggct acagcgcagc
     9841 gaaatttggt ggcgtcgggc tgactcaatc actggcgctg gatctggcgg agtacggcat
     9901 tacggtgcat tcactgatgc tcggtaacct gctgaaatcg cggatgttcc agtcactgtt
     9961 gccacaatac gcgaccaagc tgggtatcaa accggatcaa gtcgagcagt attacatcga
    10021 caaagtaccg ctcaaacgcg gctgcgatta tcaagatgtg ctgaatatgc tgctgttcta
    10081 cgccagtcct aaggcgtcgt actgcaccgg acagtcgatc aatgtcaccg gcggtcaggt
    10141 gatgttctga tcaacagcgg agatccgtta aggatctccg tgagactata gaatgcctga
    10201 tgcgctacgc tcatcaggca tacaggactt ccgccactac attaaggaaa agttatggta
    10261 tccgcactca tcaccgtcgc cgttatcgcc tggtgtgcgc aactggcctt aggcggctgg
    10321 caaatttctc gttttaaccg tgccttcgac acactatgcc agcaagggcg ggttggcgtg
    10381 gaccgttcca gcgggcgctt taaaccgcgg gtcgtggtcg ccatcgcgct ggacgatcag
    10441 cagtgcatcg tcgacacctt gtttatgaaa ggactgactg tcttcgcccg accgcaaaaa
    10501 attcccgcaa ttaccggcat gcatgcgggt gatttacagc ccgatgtgat ctttccccat
    10561 gatccactat cacagaatgc tctatcattg gcgcttaaac tgaaacgtgg ataatttcgt
    10621 tgtgaatgtt acttgcttgc gaagttatca ttttgaaacc taaatcaggt aatcacgccc
    10681 atgaaacctc gtcagcgtca ggccgccatt ctggagtatc tgcaaaagca gggtaaatgc
    10741 tcggttgaag aattggcgca atactttgac accacaggca caaccattcg caaagatctg
    10801 gtcattctgg aacatgccgg aaccgtcatt cgtacttatg gcggagtggt gttgaataaa
    10861 gaggaatccg atccgcctat cgatcataaa acgctcatca acacccacaa gaaagagctg
    10921 attgcagaag ctgccgttag ttttatccat gatggcgatt cgatcattct tgatgctggc
    10981 agtaccgttt tgcagattgt tcccctgctc tcgcgcttta ataacatcac ggtgatgacc
    11041 aacagcctgc atatcgtcaa tgcgctatcc gaactggata acgaacaaac tatcctgatg
    11101 ccaggcggaa catttcgcaa aaaatcggcc tcatttcacg ggcaactggc agagaatgcc
    11161 ttcgagcatt tcaccttcga taaattgttt atgggcaccg acggcatcga tctcaatgcg
    11221 ggcgtaacca cgttcaacga ggtttatacc gtcagtaagg caatgtgcaa tgccgcgcgc
    11281 gaagtgattt tgatggcgga ctcatcaaag tttggccgta aaagccccaa cgtagtttgc
    11341 agtcttgaaa gcgtcgataa gctaattacc gacgcaggta tcgatccggc gtttcgtcag
    11401 gcgctggaag agaaagggat cgatgtgatc ataaccggag agagcaatga gtgaagcact
    11461 actgaacacg ggacgtcaga cgttaatgct ggagttgcag gaagcaagcc gtttaccgga
    11521 acgtctgggc gatgattttg ttcgcgccgc caatatcatc ctgcactgcg aaggcaaagt
    11581 ggtggtttcg ggaattggca aatcgggcca cattggtaag aaaatcgccg caacacttgc
    11641 cagtaccggc acaccggctt tttttgtcca tccggcagaa gcactgcacg gcgatctggg
    11701 gatgattgaa agccgcgatg tgatgctgtt tatctcttac tccggtggcg cgaaggaact
    11761 ggatctgatt attccgcgtc tggaagataa atctatcgcg ctgctggcga tgaccggcaa
    11821 accgacgtca ccgctgggcc tggcggcgaa agcggtgctg gatatctccg tagaacgcga
    11881 agcctgcccg atgcaccttg cgccgacctc cagcaccgtc aataccctga tgatgggtga
    11941 cgcgctggcg atggcggtca tgcaggcgcg cggttttaat gaagaagatt ttgcccgctc
    12001 ccacccagct ggggcactgg gcgctcgctt gctgaataaa gtgcatcatc tgatgcgtcg
    12061 tgatgacgct atcccacagg tagcgttaac cgccagcgtg atggatgcga tgctggaact
    12121 cagccgcacc ggtctggggc tggtggcggt atgtgacgct caacaacagg tacaaggcgt
    12181 ctttaccgac ggcgatttac gtcgctggct ggtaggcggc ggcgcactca ccacgccagt
    12241 taatgaagcg atgacgacag gcggcaccac attacaggca caaagtcgcg ccatcgacgc
    12301 caaagaagtt ctgatgaaac gcaaaatcac tgccgcacca gtggtagatg aaaacggcaa
    12361 actcaccggc gcaattaacc tgcaggattt ctatcaggcc gggattattt aatccttcaa
    12421 tcccagacgt ttcgccagcc gatgcaggtt ggcgacgtca gtttccagca ttcgcgcaca
    12481 ggctgcccag ttgtgatgat tttgtgccag tgcctggcga atagtttcac gctggaacgc
    12541 ttctgtcgct tcacgcaggt tttgcttaac aacgggcacc gccgccgctt ctggcggcgg
    12601 caacgtcacc tcaggaaaag caaaatgttg cgcctcaaga atcacttcat cgccgttgcg
    12661 ggtggctctc gccagcacta ccgcccgatg aatagcatgt tccagttcgc gcacgtttcc
    12721 cggaaaactg tagtgttgca gtaaatttcg cgccccggca cttaatacca cgcgggagag
    12781 cccctgccgc aaacgacact gctcgcagaa ataccccgcc agcagaatga catcatcgcc
    12841 ccgctcacgc agcggcggca ccgaaagtgg aaacacgctc aggcgatgaa acaaatcggc
    12901 gcggaatcgc cctgccagca cctcttcgcg taagtcgcgg ttagtcgccg ccagcacgcg
    12961 cacatcgacc cgcaaactac ggtcatcgcc aacgcgctga atatcgccat actgcaacac
    13021 cctcagcagc ttggcctgca atgccaacga caactcgccg atctcatcga gaaacagagt
    13081 accgttatcc gccatttcaa acttcccgct gcggttactg atagcgccag taaacgctcc
    13141 tttcacatgc ccgaacaact cactttccgc cacactttcc ggcagtgcgg cacagttgag
    13201 atagaccagc ggattcaccg cccgtggcga ggcttcatga atcgctttcg ccaccagctc
    13261 cttaccggtt cccgtctcac cgctgatcag aacgttgaga tcggacgccg ccacaatctc
    13321 aatctctttt ttcagttgcg tcatgccagg ggacaagcca atcatctgcg tctgtttcac
    13381 cgcttcaaac ggcgtggcat cgcctggcag catattctgg ctttccagtt gttcaatcag
    13441 caacgcattg cttaacgctc ccgccgccag cgcggcaatc agccgtaact cttcgtcgct
    13501 gaaaacatcg aactgatcgg gctgcatccc gtcgagcgtc agtgcgccga tcaggttttg
    13561 tccgacaaac aatggcagac caacgcaggc gtgaaccttc agactctcct gcccaggaat
    13621 caaaccgtca tagggatcgg gcaattcgct gtctgcggga aagcgcacca catccccggc
    13681 gcgggcaatc gcttccagcc gtggatgccc ttccagcgca aagcgtctac cgagtacatc
    13741 ctttgccaga ccgtcgatgg caagcggaat aaactgccgc gaatcgtaac gtagcaacgc
    13801 agacgcatcg cactccagca cctgacgtag cgtggtgatc aggcgctgaa aacgatcctg
    13861 atgaccaatc ccacgctgca attcgatggc gatattcgcc agcacatcaa cggaaaaact
    13921 catctttgcc tcactgtcaa tttgactata gatattgtca tatcgaccat ttgattgata
    13981 gtcattttga ctactcatta atgagcataa ttttatttat agagtaaaaa caatcagata
    14041 agaaactggc acgcaatctg caattagcaa gacatctttt tagaacacgc tgaataaatt
    14101 gaggttgcta tgtctattgt ggtgaaaaat aacattcatt gggtgggtca acgtgactgg
    14161 gaagtgcgtg attttcacgg cacggaatat aaaacgctgc gcggcagcag ctacaacagc
    14221 tacctcatcc gcgaagaaaa aaacgtgctg atcgacaccg tcgaccataa attcagccgc
    14281 gaatttgtgc agaacctgcg taatgaaatc gatctggcgg atatcgatta catcgtgatt
    14341 aaccatgcag aagaggacca cgccggggcg ctgaccgaac tgatggcgca aattcccgat
    14401 acgccgatct actgtactgc caacgctatc gactcgataa atggtcatca ccatcatccg
    14461 gagtggaatt ttaatgtggt gaaaactggc gacacgctgg atatcggcaa cggcaaacag
    14521 ctcatttttg tcgaaacacc aatgctgcac tggccggaca gcatgatgac ttacctgaca
    14581 ggcgacgcgg tgctgttcag taacgatgct ttcggtcagc actactgcga cgagcatctg
    14641 ttcaacgatg aagtggatca gacggagctt ttcgagcagt gccagcgtta ctacgccaat
    14701 atcctgacgc cgttcagccg cctggtaacg ccgaaaatta ccgagatcct gggctttaac
    14761 ttaccagtcg atatgatagc gacctcccac ggcgtggtct ggcgcgataa cccgacgcaa
    14821 attgtcgagc tgtacctgaa gtgggccgct gattatcagg aagacagaat caccattttc
    14881 tacgacacca tgtcgaataa cacccgcatg atggctgacg ctatcgccca gggaattgcg
    14941 gaaaccgacc cacgcgtggc ggtgaaaatt ttcaacgtcg cccgaagcga taaaaacgaa
    15001 atcctgacca atgtcttccg ctcaaaaggc gtgctggtcg gcacttcgac gatgaataac
    15061 gtgatgatgc cgaaaatcgc cgggctggtg gaggagatga ccggattacg cttccgtaac
    15121 aaacgcgcca gtgctttcgg atctcacggc tggagcggcg gtgcgatgga tcgcctttcc
    15181 acgcgcctgc aggatgcggg tttcgaaatg tcgctcagcc tgaaagccaa atggcgacca
    15241 gaccaggacg ctctggagtt atgccgtgaa cacggtcgcg aaatcgcccg tcagtgggcg
    15301 ctcgcgccgc tgccgcagag cacggtgaat acggtagtta aagaagaaac ctctgccacg
    15361 acgacggctg acctcggccc acgaatgcag tgcagcgtct gccagtggat ttacgatccg
    15421 gcaaaaggcg agccaatgca ggacgttgcg ccaggaacgc cgtggagtga agtcccggat
    15481 aacttcctct gcccggaatg ctccctcggc aaagacgtct ttgacgaact agcatcggag
    15541 gcaaaatgag caacggcatt gtgatcatcg gttcgggctt cgccgcccgc caactggtga
    15601 aaaatattcg caaacaggac gccactattc cattaaccct gattgccgcc gacagcatag
    15661 atgagtacaa caaacccgac ctcagccatg ttatcagtca ggggcaacgt gccgacgacc
    15721 ttacccgcca gacggcgggt gaatttgccg ggcagtttaa tctgcgcctg tttccgcaca
    15781 cctgggtgac ggatatcgat gccgaagccc atgtggtgaa aagccagaat aatcagtggc
    15841 aatacgacaa gttagtgctg gcaaccggtg ccagcgcctt tgtcccgcct gtgcctgggc
    15901 gtgagttaat gctgacgtta aatagtcagc aagagtatcg cgcctgtgaa acgcaactgc
    15961 gggatgcccg acgcgtgttg attgtcggcg gtggtttgat tggtagcgaa ctggcgatgg
    16021 atttttgtcg tgcaggcaaa gcggtcacgc taatcgacaa cgctgccagt attctggcgt
    16081 cattaatgcc accagaagta agcagccgct tgcagcatcg gttgacggag atgggcgttc
    16141 atctgctgtt gaaatctcag ttgcaggggc tggaaaaaac ggattctggc attctggcaa
    16201 cgctggacca ccagcgtagt atcgaagtgg atgcggtaat tgccgccacc ggactgcgcc
    16261 cggaaacagc cctggcacga cgcgccgggc tgacgattaa tcgcggcgtt tgcgtcgata
    16321 gttatctgca aaccagtaat gccgatattt acgcgctggg cgattgcgcg gaaattaacg
    16381 gtcaggtatt gccgttcctg cagccgattc aacttagcgg gatggtgctg gcaaaaaatc
    16441 ttctcggcaa taacacgccg ctgaaactac cggcgatgct ggtgaaaatc aaaacgccgg
    16501 aattaccgct gcatctggca ggcgaaaccc agcgtcagga tttacgctgg cagattaata
    16561 ccgaacgcca gggaatggtg gcgcgcggcg ttgacgatgc tgaccagctt cgcgcctttg
    16621 tggtcagtga ggatcggatg aaagaggcat ttgtattgtt gaaaacgttg ccggtgtagg
    16681 tgggctactg tgcctaaaat gtcggatgcg acgctggcgc gtcttatccg acctacgggg
    16741 acgcatgtgt aggccggata aggcatttac gccgcatccg gcaagggtgt ccaaatgcaa
    16801 cacgctttat ccgttctgga cttcacccgc taaccaacgc gccgcagcaa taaccccctg
    16861 ccccagagac aaaccgccat cacccgccgg taaactctgt ggaaagagca atgtgaaatc
    16921 agcgagataa tgcgccagac gtgcacgcag caaacggtta tgaataaccc cgccgctaaa
    16981 taccagcgta gtgataccac gcatcgtggc ctgctcacgc atcaacgcgg caaaaccctg
    17041 cgccagcgca tcatgaaacg cccacgcgcg ttgattaacc agtgcctgcc agttcagcca
    17101 ctgctgccag aaagttgcga gatccagttg attgtccacc cgcggcatcg tcaccggatg
    17161 cgtcactccg tcgcatgagg ctgcgagcgc ctccagagca caagccgctt caccttcata
    17221 acttaacgtg gctggcgcac agcccagtgc cgccgccact gcatcgaaca accgcccaca
    17281 cgatgatgcc agcggcgcgt taattccacg ctcaatggcc cgcgccagta cgctccagtt
    17341 ttgctgttgc acacttgccg tttcagggta attctgccac tccggcacaa agcgcaggca
    17401 ctgcgccagc aggtttcgcc acggctgctt cgccgccaaa tcgccacccg gaagcgccac
    17461 tgcaggcaag ccgcccaggt gctcacattc gcgatagttc acccgcaggc actcgccgcc
    17521 ccacaaagcg ccattctccc ccataccgat accgtcgagc gtcaaagcaa tgacatcacc
    17581 gccatccagc ggccactgat gctctgccag acacgccgct gcatgggcat gatgatgcag
    17641 taccgtttgc gtcggcagat tcatttcgct cgcccactgg ctggagacat agcccggatg
    17701 cacatcatgc acaatgtatt gcggagtgaa atcgtagatg ttttgcatca ggcgtaacgc
    17761 ttcgcgccac tgcatctgga tgccatcgtc acttaaatcg cccagatgct gactcaacac
    17821 cgcttgttca ccgcgcacca ggcagaaggt atttttcaga tccgcgccaa gacacagcac
    17881 aggcggaaca tttttaaagc ccggaggcaa aaccagcgca tccggcacat acccccgcga
    17941 acggcgcagc atttcgccgc tttcgcgcac cactgaatca tccatccgct gcacgatgtc
    18001 gcggttatgt agcaagaatc cgtcggcaat gccctgcaaa tccgccagcg cctgttcgtt
    18061 gctgatagct ggtggtttac cgctcaggtt gccggaggtc atcaccagcg ggcattgcag
    18121 ttcctgtaac agcaaatgct ggagcgggtt cgcaggcaac atcaccccga cttcgttaag
    18181 gccaggggcg atatcatcac aaagctcagg tacgtatttt ttatccacca gcacaatcgg
    18241 cgcagcgggc gtggtaagca actggcgcgc agcgtctggt aaactttctg ccactggcaa
    18301 catgactgcc agcggtttcg ccgggcgatg tttgcgctcc cgcagtgtcg ccaccgcgtt
    18361 actgttacgt gcatcgcagg caagatgaaa tccgccaatc cctttgatgg cgacaatgtt
    18421 gcccattttt aactgtgcga tagctgcctg taatgccgcc tcttgttctg catgttcacc
    18481 atgacttacc cattcaagat gcgggccaca ctccgggcag gccaccggct gggcgtggaa
    18541 gcgacgatcg agcgggtcgc ggtactcttt gtcacaggcc ggacatagcg gaaacgccgc
    18601 catcacggta aacgggcggt cgtaaggcat cgcgcgaata atggtgaaac gcgggccgca
    18661 gtgggtacag ttgataaacg gataacgata acgccgctcg cctggggtat tcatttcggc
    18721 aaggcaagca gggcaagtgg cggcatccgg gacaatttgc gtattcatgg tgccgccggt
    18781 gctctggcgg atggtgaact cggtgggcag ttgtgaccag ataaacggct cacgctcgac
    18841 gctatcaata cgcgccagtg gcggacagtg ctgatgcaat tgaacaagaa acgtttccgg
    18901 gtcttccagc agccggactt ctacgccatc gccgtcatta catacatcgc cgtgaagatt
    18961 taattgctgt gccagctgcc agacaaacgg acgaaaaccg acgccctgca ctttgccacg
    19021 aatacgcagt tggacaccgc aagatgtgtt ttttgccatt gagttattcc cgccatcatg
    19081 aattgcgtaa cccgccctgc cggacacgac agcgtcgtat ccggcagtct caggtcggcg
    19141 ataccgcccg ctccgtattc tacgaatatt tccgggaatt cctttgatgc cagaacagtt
    19201 ctgtaagatt tttagaacat cagcgccgta cggcggcgtt tttctgcgct cagttgttca
    19261 agtttattac gatcgacaca aatcagcgca tgggtcgggc aagccgccat acacgccggg
    19321 ccgtcttcac gatggttgca caggtcgcat ttattggctt cggctttgtc agcccgtaca
    19381 ttcagacccg cgccgctgtt gcgaatcaca ggacgtacca ccacttccat cgcgccatac
    19441 gggcaagcca ccacgcaggt tttgcaacca atgcaacgtt cctgcatcac aggaacaaac
    19501 cctttatcac ggctgatagc accattcggg cagacgttag cgcacggtgc atcttcacac
    19561 tgacggcaaa ctgtcgccgt ggaaatgttc acacctttaa tgacatggat acgcggtaaa
    19621 aatgtttccg gggtcagcga tgcacagtcc tgattttcct gatgagaaac cacgcacgcc
    19681 acttcacagg tacggcaacc aatacattta ctcgcgtcag caatgatgaa acggttcatc
    19741 aaattctcca gcaatgacag ttaatgcgcc gatacactca caaatcatgc cagtttttaa
    19801 tttgctgtta tttaaggaaa ttaatttctg taatgcagga aaaaagatgt catcgacact
    19861 agtgacgatg acatgtgatg acaatgttta tcgcgaaagg gcaatgagtg agtcgcggcg
    19921 gatcagtttt ccgctaaagg ttttcggcgg tgagaaatcc ccgccatcga gcataaaaat
    19981 cagccgtcca ataatttcct gaatcatctc agtcaccgga atttttacgc tggagagcgc
    20041 cggaacgata tagggggcaa ttgcgatatc atcgaatccg ataactgaca cctgctctgg
    20101 caccgctacg ccgcgctcgt gcaacgcttt gatcgcacct atcgccatat cgtcgttact
    20161 ggcaactaac gcgctaaatt tagcccctcg ttcgagcaac gtttctaccc cttcggcccc
    20221 gctggcgggc gtccatttac cgttagcgat aagtttttca ttgagcgcaa taccatgctg
    20281 cgacagcgcg tctttatacc cggcaagacg ttcaatgctg gtgggggaat ccatcgagcc
    20341 ggtaaggaaa gcaatctcct gatgcccggc gtttatcaac tctgccacgg cgttaaagct
    20401 ggtctgttta tgatcgcacc agacgctatg gctgctatta ttgcgcaggc ggcgattaag
    20461 caccattatc ggctgactgt gcgcgtcaat gatgtcatca atctcatcca cgcttaaaaa
    20521 acgcgggtaa atcataatgg cgtcgcagcg caaatccagc agatactgaa tcgcctggcg
    20581 ctcttcttcg gcgctgtgtt taccatctgc caatagcaac tgtcgccctt tctcttccgc
    20641 cattcgcgcg gcatgaaaca gtaattcact aaaataaatg ccgtgataaa gcgtgttggt
    20701 cactaccagc cccagcgtct gagtactctt cgccgacaga ttgcgggcca gcaaatttgg
    20761 gcggtaactg ctctcttcta ccgcctgaaa cacgcgatct ttagtctcct ggctgacgta
    20821 gccattacct gaaagcacgc gggaaacggt cgcttttgaa accccggcgc gcttcgccac
    20881 ttccagcatc gtcgtcatca ttttcatccc tttacacgca atcaacgcag tgtactgcac
    20941 cgtttgccga ttgtccttgc gcaatcggct ccagcggata agacgaggtc gccgtgccgg
    21001 ttttgatgac ttttttgata aaggtaaaca tggcgattcc ttatttcagc ggcgagtttt
    21061 tacgctcaat gctgtaacgc tcgagttctt tgtacggcac cactttgctc ttcttcttac
    21121 gcacatcgac tacggtcatg cggtcggtac aggagtagca agggtccagg ctaccgataa
    21181 tcagcggcgc ataggaaacg gtattgccac gcagcatgta gcgcagggtc ggccagttcg
    21241 cgtaggtcgc ggcacggcag cgccagcggt acagcttctg gttgtcgccg gtcatgctcc
    21301 agtggatatc gtcgccgcgc ggcgcttcgg caaagcccag cgcgaaacgg tgcggaatgt
    21361 aggtaaagcc ttccaccatc agcggaccgc ccggcaggtt atccagaccg tagtcgatca
    21421 tattcagcgc ggtatagacc tcgttgattc gcactttcag acgggaaata acgtcgcagc
    21481 cctgctcgct gtggacttcc attggcagca ggccataacc gacaaacggg tgatcggcgc
    21541 gagtatcgcg ggcgagaccg ctggcgcgaa ccatcgggcc aacgttactg aaatcgcggg
    21601 cgatttccgg gtccagacga ccaatgccga cggtgcgttg ttccatgttc ggcgtactca
    21661 gcagcacatc caccagctcc tgcacttcac gacgcatttg ttgcgccagc tggcgggtct
    21721 ggatcatgtc atctttcagc agatcgcgac gaatcccgcc gatcaggttc aggccgtagg
    21781 ttttacgcgc accggtaagg atctctgcca ttttcatgga ggtttcacgc acgcggaaga
    21841 actgcataaa gccggagtcg aagccggtaa agtgacaggc gaggccgagg ttgagcagat
    21901 gcgggtgcag gcgttccacc tccagcagaa tggcgcggat catctgcgcg cgttctggca
    21961 cctgaatacc catcgcgttt tccaccgacg tggtgtaggc ggtgctgtgg gcaaagccgc
    22021 agatcccgca cacacggtca gagaggaagg tcacttcgtt ataacccatg cgggtttccg
    22081 ccagtttttc cataccgcga tggacgtaga acagacggta gtcggcgtcg ataatgtttt
    22141 cgccatcgac gaacagacgg aagtgacccg gttcgtcaga agtgacgtgc agcggaccaa
    22201 tcggcacgac gttgtttttc ttgtcgccca gttcgttgat gaactcgtag gtttcagcat
    22261 cggtagtcgg tgccggacgc tgacgataat ccatgctgtc tttacgcagc ggataaagtt
    22321 catccggcca gtcatccggc agcaccagac gacgttcatc cggcagacca accggaatca
    22381 aaccgtacat atcgcgcact tcgcgctcgc cccacaccgc cgccggaacg cgcggcgtca
    22441 cggacggata ctccggtttg ttggcgtcaa cttcgacgcg aaccgttacc caacacttgg
    22501 tgcccttctc catcgacagc acgtaataaa cggcgtaatg accattcagt ttgcgttcgt
    22561 cgttaccaaa cagcaccgac agccagccac cctgcttgta gtagagaaac tccaccactt
    22621 ccggcagata gttcaccttt acggtgacag tcagctgatc tttggtctgc caggcgtggt
    22681 ccagcacgac gcctagaaat gcctcattca gcgcggcgag ataatgttga cctaattttt
    22741 cttcagacat gctcaaactc tctttaatca cgccgccagc aaggagacga acgctaaaaa
    22801 tgcaaagcca aacccagccc aggtaatgcg cggagtaata tcaagacgca gacgggccat
    22861 gctgttttcg aacagcgcga taaccaggac gccgaccacc agtttgacga tggcaatcac
    22921 cagcgccagc agcagtccac cgacggtgaa ggtttccatt tgtccccacg gaataaacac
    22981 cccgacgaac atctgcaaca ccaccagctg tttcaggctg ataccccatt tcatgacgcc
    23041 aaagccgcta ccgctgtatt cggagagcgg accttcctgc aactcctgct cggcttccgc
    23101 caggtcgaac ggcagtttgc ccatttcgat aaaagtggcg aacgcacagg cgcagagcgc
    23161 cagcaccagc gggatgcttt ggctcagcgg ccagtgataa acggtgtcgg taatgttgct
    23221 gatgttggtg gaaccggcaa cctgtgcggc aacccacaga ccaagcagca acatcggttc
    23281 gaccagcacg ccaagcatcg cttcacggct cgcaccgata gcggtaaacg ggctaccggt
    23341 atccagaccg gaaatggcaa agaagaaacg cgcgatggcg aagagataca gtaaggtgat
    23401 caaatcgccc agttgcggca gcggagaacc gacggtcacc accggcagcg cagtagcgat
    23461 agtcagcatg acgcccacca tcacatacgg cgtcaggcgg aacatccagc cagaggcatc
    23521 cgggccaacg ctctggcggc ccagcagttt gataatgtcg cgatactcct gcaacacgcc
    23581 cggcccgcga cggttatgca gacgggcgcg cgccacgcgg gtaataccgg agagcagcgg
    23641 cgcaacggca aataacacca gcgcctgaat taacggatat aaaacactca ttctcaggct
    23701 cctcgtgaaa caataatcac caccagtacc gccagttcaa ccagcgccat ccggcggaac
    23761 agcaacgcac taccttcgca ctgccagccc ggcactagcg acaccggatt cagccatttg
    23821 cgcagtttca gcactggcgc aaacgcctgt ttaaccggca tggcaaaacc gtgagcggta
    23881 atcaccattg atttttcatg gtcgtagccg cacacccagg ccgcaccgcg ggaacgtgac
    23941 ggcaggcggt cgcctttgca aatcgccata atgatgaatg gcagcagcgg gcaggcaatc
    24001 agcagcaatg tgatcattgg ttgagaaacg gtggtgttag caggctccag aggcagaggt
    24061 acagcagcag agagcatcgg cagcagccac ggcgcagcaa caccgccaat tacgcagcaa
    24121 atcgccagtg ccactacgct tacgctcatc aggagcggcg cacaggtggc gttttcggct
    24181 tctttggtac gcggcgcgcc gaggaacgtg acgccataga ctttcgccat acacatcacc
    24241 gccagcgcac cggtaattgc cagacccaca gcgagcagcg gccccagcag acgggcaaca
    24301 aacgcgccgc tattgctcag tttgaagaag gattgataga taacccattc cccggcaaac
    24361 ccgttcagtg gcggcagcgc agccattgcc atcaacccga ctaacatggc gatggagata
    24421 accggcattt tcttgccaat accaccgagt ttttcgatat cgcgatgacc ggtacggaac
    24481 cagacgctcc ccgccccgag gaacagtacg cttttgaaca ggctatggtt aagcagatgg
    24541 tacagaccac cgaccaggcc aagagcaatc agcgccggtt gttcgagcgc g
//
LOCUS       DAZSIO010000046        24498 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZSIO010000046 DAZSIO010000000
VERSION     DAZSIO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.046"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..776)
                     /locus_tag="ACHX5T_001718"
     CDS             complement(<1..776)
                     /locus_tag="ACHX5T_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFR8466953.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIRLIILFLLAA
                     LSFIGLFFMINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLKNT
                     EEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGR
                     VIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKI
                     HVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLARE"
     gene            complement(876..1787)
                     /gene="gsiD"
                     /locus_tag="ACHX5T_001719"
     CDS             complement(876..1787)
                     /gene="gsiD"
                     /locus_tag="ACHX5T_001719"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR8466954.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1790..2710)
                     /gene="gsiC"
                     /locus_tag="ACHX5T_001720"
     CDS             complement(1790..2710)
                     /gene="gsiC"
                     /locus_tag="ACHX5T_001720"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR8466955.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2728..4266)
                     /gene="gsiB"
                     /locus_tag="ACHX5T_001721"
     CDS             complement(2728..4266)
                     /gene="gsiB"
                     /locus_tag="ACHX5T_001721"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR8466956.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4286..6157)
                     /gene="gsiA"
                     /locus_tag="ACHX5T_001722"
     CDS             complement(4286..6157)
                     /gene="gsiA"
                     /locus_tag="ACHX5T_001722"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR8466957.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6144..7109)
                     /gene="iaaA"
                     /locus_tag="ACHX5T_001723"
     CDS             complement(6144..7109)
                     /gene="iaaA"
                     /locus_tag="ACHX5T_001723"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR8466958.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7313..8548
                     /gene="moeA"
                     /locus_tag="ACHX5T_001724"
     CDS             7313..8548
                     /gene="moeA"
                     /locus_tag="ACHX5T_001724"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR8466959.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            8548..9297
                     /gene="moeB"
                     /locus_tag="ACHX5T_001725"
     CDS             8548..9297
                     /gene="moeB"
                     /locus_tag="ACHX5T_001725"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR8466960.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9476..10138)
                     /gene="fsa"
                     /locus_tag="ACHX5T_001726"
     CDS             complement(9476..10138)
                     /gene="fsa"
                     /locus_tag="ACHX5T_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR8466961.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            10269..11168
                     /locus_tag="ACHX5T_001727"
     CDS             10269..11168
                     /locus_tag="ACHX5T_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR8466962.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11174..13606
                     /locus_tag="ACHX5T_001728"
     CDS             11174..13606
                     /locus_tag="ACHX5T_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR8466963.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13752..14567
                     /gene="ybiV"
                     /locus_tag="ACHX5T_001729"
     CDS             13752..14567
                     /gene="ybiV"
                     /locus_tag="ACHX5T_001729"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR8466964.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14719..15984
                     /locus_tag="ACHX5T_001730"
     CDS             14719..15984
                     /locus_tag="ACHX5T_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR8466965.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(16225..17817)
                     /locus_tag="ACHX5T_001731"
     CDS             complement(16225..17817)
                     /locus_tag="ACHX5T_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR8466966.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            18036..18956
                     /gene="ldtB"
                     /locus_tag="ACHX5T_001732"
     CDS             18036..18956
                     /gene="ldtB"
                     /locus_tag="ACHX5T_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR8466967.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(19014..20132)
                     /locus_tag="ACHX5T_001733"
     CDS             complement(19014..20132)
                     /locus_tag="ACHX5T_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR8466968.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(20129..20596)
                     /gene="mntR"
                     /locus_tag="ACHX5T_001734"
     CDS             complement(20129..20596)
                     /gene="mntR"
                     /locus_tag="ACHX5T_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR8466969.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20782..20910
                     /gene="mntS"
                     /locus_tag="ACHX5T_001735"
     CDS             20782..20910
                     /gene="mntS"
                     /locus_tag="ACHX5T_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR8466970.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            21182..22765
                     /gene="opgE"
                     /locus_tag="ACHX5T_001736"
     CDS             21182..22765
                     /gene="opgE"
                     /locus_tag="ACHX5T_001736"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR8466971.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22813..23328)
                     /gene="ompX"
                     /locus_tag="ACHX5T_001737"
     CDS             complement(22813..23328)
                     /gene="ompX"
                     /locus_tag="ACHX5T_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR8466972.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(23381..23446)
                     /locus_tag="ACHX5T_001738"
     CDS             complement(23381..23446)
                     /locus_tag="ACHX5T_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8466973.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23681..>24498
                     /gene="rhtA"
                     /locus_tag="ACHX5T_001739"
     CDS             23681..>24498
                     /gene="rhtA"
                     /locus_tag="ACHX5T_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR8466974.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAI"
BASE COUNT         5994 a         6646 c         6071 g         5787 t
ORIGIN      
        1 ttttcacgag cgagttgatt cttggcaatg atctcatgac cttgtttcaa aaatagaact
       61 tcatcattag cgctgatttg atcaagatac tcgttcagat accgccagtt taaatcgaca
      121 tgaattttat ctaccgctaa caaatcgtga tctttgccgt tgatacgtag cgtaaaagag
      181 atacattttt tacccggacg atcaaagcaa ttgtatgccg gattccagct gttgttagag
      241 tcatctgaag ccaccgacca cggtcgtttc gatgggttaa aatcggcaaa aataacccgc
      301 cctgactcgt cgacaagcgg tccatttact tgttcctgat gataaacaac gattttatca
      361 ttcgccctcc gcgaaaagag ataatattta ttatcatcaa gtaccagtcc caccgagtct
      421 aaaaatggca tcatttccat tttcagaatc acggcgcgtt gaatctcctc tgtatttttt
      481 aataaataca gtgagtcctt taatgtcagg gccgaacgtt caatatcttt aaagaaatag
      541 ccaacgtttt tctgaataag ttcaaagcgg ctatcggcac gttttaccgc gcgttccgat
      601 accagttgat agttaatcat gaaaaaaaga ccaataaatg acagtgctgc caataaaaat
      661 aaaattatca gccttatctt ttcgtataaa ctcagcatgt taatacattt aacaaaataa
      721 ctatctgaac gctgtgccgc tgcaaaaata gtcccttgct gcggcttttt aaccaaatta
      781 cccgattcta caaaatcagc agaaagttac aacacttgct tagggcaata atttcctgtg
      841 aggtgattac cctttcaagc aatattcaaa cgtaactatc ctttaatttt cggatccagc
      901 gcatcgcgta aaccatcgcc caacaaattg aacgccagta cggtcagaaa aatagccagg
      961 gccggaaaaa cagcgacatg cggcgcgata accatatccg ctcgtgcctc attgagcatt
     1021 gctccccact ctggtgtcgg cggctgcgca ccgaggccga gaaatgacag gctggcggca
     1081 gagataatcg aggtgccaat gcgcatggtg aaaaacacca cgatagaaga gacggtccca
     1141 ggcaggatat gacgcaataa aatggtcata tcgctggcac caatactgcg tgctgactca
     1201 ataaaggttt gctgtttcaa caccagcgtg ttgccgcgca ccaggcgggc aaacgcgggg
     1261 atggaaaaaa tggcgactgc gataatcacg ttagcaatgc cgcttcccaa caccgcaaca
     1321 acagcgatcg ccagtaaaat acccgggaag gcaaacagca catcgcaaat gcgcatgatc
     1381 agccgatccc accagccttc ataatatcca gccaacaagc ccagcaacgt ccctatcgcc
     1441 acaccgataa acacggcaaa cacgcccgcc gccagcgaga tttgcgcacc aaccaggaca
     1501 cggctgaaaa tgtcgcgccc cagtgaatcg acgccaaacc agtgctgcaa agaaggtccg
     1561 ttattcagat tgtcataatc aaaataattt tcggcgtcat agggagcgat ccagcgtgca
     1621 aaaatggcca ccacaatcaa taaaataacg aataatgcgg cggtcatcgc catatgctga
     1681 cggcgaaatc gtcgccagaa ttcatgccac ggtgtacgta cctggtcagg tttgaccagt
     1741 ggcatggcgt ttaacaccgc ctgacgtcgc cagttaaata gtcgcatcct tacttgtacc
     1801 tgatagccgg gttaatggcg gcgtaaagca catccaccac taagttgata agaataaatt
     1861 ccagcgagaa aagcagaatt tccgcctgaa tcaccgggta atcgcgcatt tctacagagt
     1921 caacgagtaa gcgtccaagt ccgggccagt tgaaaacttt ctccaccacg atagaaccac
     1981 cgagcaaaaa gccaaactgt aagcccatca tggtcactac cgggatcatc gcgttgcgta
     2041 gcccgtgttt gaggacaacc caggtttcgc tcaccccttt cgccctcgcg gtacgcatat
     2101 aatcttcgct taaaacgtcg acaaatgacg cacgggtaaa gcgcgccatc acggcggcca
     2161 ctgccgcgcc gagcgtcagg gagggtaaaa tgtagtgctg ccaactgtct gctcccacag
     2221 taggcagcca gcccagttca acggagaata cctgaattaa aaacatcccc agcgcaaatg
     2281 ccggaaacga gatccccgac accgcaatgg tcatactcaa tcgatccggc caacgattac
     2341 gccagacggc ggcgataatt cccgccgcca taccaaatat caccgcccag accatactgg
     2401 ttatggtcag ccatagcgtt ggcataaagc ggctggcaat ctcatcggca accggacgac
     2461 gcgacaccat cgacaggcca aaatccccct gcacagcatt gctgatatag tgccagaact
     2521 ggtgatacag cggctgatcc aaccccagct gctgacgcac cagttctata acctgcgcat
     2581 cagcttcggg cccggcaatc aatcgcgccg gatcgccggg cagcatgtgg acaaataaaa
     2641 acaccagcac cgagacgata aacagcgtcg gaatcaaccc cagtaatcgt ttgataacgt
     2701 aataaagcat tccactccct gcgttgctta ttgcaaatcc gcgtcttcaa agctgaagcc
     2761 ggtatctggc atgatccaaa aaccggtcag gtttttactg tgtgccgaca ccagtttttc
     2821 taccaccagc gggatccacg gcgattcttg ccagatgata tcctgcgccg ccttatataa
     2881 gcgggtcttt tccgccggat cattcgtttt cagtgcctga gccaggaagt catccacctg
     2941 tttattgctg taaaacgcgg tattaaacag cgtcggtggc cagttttgcg aggcaaacag
     3001 cggcgatagt gcccagtcgg cttcgccggt tgaagccgac cagccagtgt agaacatccg
     3061 cacgccactc tctttttgcc ctttaccttc aacttctgcc gcccgctgtc cggcatccat
     3121 cgcggtcacc tgggctttaa tcccgacctg cgctaactgc tgctgggtaa attgcagcac
     3181 tttctgcgcg gtgctgtggt tatgtgacga ccacagcgtg gtactgaaac cgttgggata
     3241 tcccgcctct ttcagtaatt cgcgcgcttt cactggatcg taaggccacg gtttataact
     3301 ttgcgcgtag gcgatacttg gcggtaccac accagtagct ggagttgcat agcccgcaaa
     3361 ggcaactttc accagcgccg gacggttaat ggcgtaattc agcgcctcac ggaccttcgg
     3421 gttatcgaac ggcttttgcg tcacgttcat actgatataa cgctgcataa ttgacggact
     3481 ggccatcaac tcgatatttt tgtttttctc cagcagtgtg gcctgctcgt agggaatggg
     3541 gaaagcaaac tgcgcttcac cggtttgcag cattgccgcg cgggtgttgt tatccgccac
     3601 cggacgccag gttatgctgt ccagtttggg caatcctggc tgccagtaac ccgcgaattt
     3661 tttcaccttc acaaaatcgg tctgattcca ggtatccagt tcatacggtc cggttcccac
     3721 cggatgaaaa ccaatctcct tgccatattt ttccagcgct gtcggtgaaa tcatcgcggt
     3781 cgccggatgg gcaagaatat taataaacgc tgagaacggc tgtttgaggg taatctttac
     3841 cgttgtcgga tcgatcgcct ccgttttggc aatattctta tacaggttat agcgtttaag
     3901 atgattcgcc ggatcgctgg cccggtccag attcgctttt accgccactg cgttgaaatc
     3961 ggtgccatcc tggaatttaa ttccttcccg caatttcacg gtgtaagtaa tgccgtcatc
     4021 ggaaacggta taactctccg ccagcacgtt tttcagtttc atctctttat ccagaccgaa
     4081 cagcccctgg taaaacgatt tcgctacggc ctgagataac gtgtcattcg cgtcatacgg
     4141 atcgagcgtg gtgaaattcg atcctaccgc caccaccaca tctttggcag cgaatgcaca
     4201 agatgccatc aacgctgtcg caatgcccag cgccactaac ccactacggt gtacagctct
     4261 tgccatttta ttctccgcct gaatgttatc tacgcatgaa tgcgtattct gattgtggtt
     4321 gtgcgacgta atgccccggc ccgacgcatt gcaacgagac ggctgccacc tcttcgccac
     4381 gcagatgaat attgctggga agatcgtccg acagcagtac acgctgcggt cgttgtcggg
     4441 acggttcagc gaccggaact gccgccagta atttacgcgt ataaggatgc tgcgggtttt
     4501 cgaagaccgc gcaccgtgaa ccaatttcaa caatttgccc gagatacatc accgccacac
     4561 gatgactaat ccgctctacc acagccatat cgtgggagat aaacagatac gcaatgccga
     4621 aatcacgctg gagatcgagc aacaagttga taatctgccc gcgaatagaa acatccagcg
     4681 ccgaaacggc ttcgtcggca atgatcactt ttggattcaa tgccaacgcg cgagcaatgc
     4741 agatgcgctg gcgctgaccg ccggaaaact catgcgggta acgccaggca tgttcaggta
     4801 acaggcccac gcgctccagc aaccacgcaa cgcgtgcagc cgcgtcttta cctggcaata
     4861 aaccgtgtac acgcagcggt tcgataatcg aatcaccgat ggtctgacgt gggtccagcg
     4921 aagcgtaagg gtcctgaaaa ataaactgaa tatcccggcg caatgcctga agtttgccag
     4981 gtgacaaggt atcgattcgc tgaccgttaa agataatttc gccgccctgc gattcgacca
     5041 ggcgcagcaa cgcccgcccg gtagtggatt taccgctgcc agactcgccc accagcgata
     5101 gcgtttcgcc aggccagaga tcaaaactga ctttctcaac ggcatgcact tcccgcgtta
     5161 cgcgattcaa caaaccgctg cgcaaaggga aacgggtgac aagattacgc acccgtaaaa
     5221 caggttcgcc atccaccacc gttttctgct cgatgggggg ttcctgtttc gctggatgtt
     5281 caagcgatat caatgggaaa cgtcggggat aatctaaccc tttcatcgca ccaagttgcg
     5341 gaacagcagc taacagcgca cgggtgtaag gatgttgcgg tgcatgaaaa atctgttcga
     5401 cggtacccgt ttccaccgcc tcgccctgat acatcaccag tacccgatcg gcaatctctg
     5461 ccaccacgcc catatcgtga gtgataaaga taacgcccat cgacatctct ttttgcaata
     5521 ctttgattaa ttgcaggatc tgcgcctgaa tagtgacatc cagcgcggtg gttggctcat
     5581 cggcaatcag caccgccggg cggcatgaca gcgccatcgc aatcatcact cgctggcgca
     5641 tcccgccaga gagttgatgc gaataacgtg aaagaatggt ttgtgcctcc ggaatgcgta
     5701 cctgatccag catccgcttc gcctcgacca tcgcttcttc acgactggcg ttctgatgca
     5761 gacgaattga ttcggcaatc tgttcaccca cagtaaatac tgggttcagc gatgtcatcg
     5821 gctcctgaaa tatcatcgcc atatccgcac cgcgcacatg gcgcatttgt gcagcgctct
     5881 gctcgctaag ttcaatcaca tcacgactgc gccgccgcaa cagcatttta tcgcactgta
     5941 ctaaaccgcc cgcctgttcc aacagacgca tcaatgccag cgcagtcact gacttaccgg
     6001 agccggattc gcctacaatt gccagcgtct caccgcgttg cagactaaaa gagagattgc
     6061 ggaccgcagc tattttctgc tggtcctgca taaaggcaat attaagattt tcaaccgcca
     6121 gcacattacc ggcatcaagt tcatcactgt gtggcaacgg tgtccccttt ttcacggtag
     6181 ataccggtgg ttggcgtatc gcctgcgtag ccccaggcgc gatacattcc ttcggtgtta
     6241 aacggtagcg cgacattccc ttcatggtcg atagcgatta agccaccgct accgccaagc
     6301 gcagggagtt tttccattac tacccgctcg caggcttccg cgagacttaa tccgccgtaa
     6361 tccattaacg cggcgatgtc atatgccgcc agcgcgcgga tgaagacttc gcctgtgccg
     6421 gtacaagaaa ccgccacact ggcgttattg gcgtagcatc cggcacccac taagggacta
     6481 tcgccaactc gtccgggtaa tttattggtc attccgcctg tggacgtggc tgccgccaga
     6541 ttgccgtcta aatccaacgc cacggccccc acggtgccca ttttctgttt ttcatccagt
     6601 ggcgcaccgc tatggtcgag gactgttgcc ccttcctcgc gcgctgccag tagttgttca
     6661 taacgcaaag gcgtggagaa aatctccggt gagacgcgct ccatgccatg agcaaacgca
     6721 aaattttctg ccccttcgcc aatcatcatc acatgcgggc tttgctccat caccagccgg
     6781 gcggcaagaa ccggattacg cagatgacta acgcccgcca ccgcaccggc tttcagggtg
     6841 ttaccatcca tcacacaggc gtccagttca tgggtttcat cacgcgtaaa gacagcgcca
     6901 attccggcgt taaacagtgg acactcttcc agcagacgca ccgcttccgt caccacatcc
     6961 agcgcacttt cgccggcttc cagcattttc tgcccggttt caacaatggc agacaacgcc
     7021 tcgatgtagc gtaattcctg ttgcagactc atctgcgcgc ggctaattgc acctgcgcca
     7081 ccatgaattg caatgactgc tttgcccata gtattcgtcc attaagggtg gctcgccact
     7141 tttgctataa atatcagaat ttttatgaat tactcattca atttttgaat atagaaagat
     7201 ggatatggca tgtaaaggca ggagccaacg ctacgcccga ggtggcatac agtctatggc
     7261 gtttttctga cataataggc aaattcgatt ttgcctccgc aggagtgttt tcatggaatt
     7321 taccaccgga ttgatgtcgc tcgacaccgc gcttaatgag atgctttctc gcgttacccc
     7381 actgaccacc caggaaacgc tgccactggt tcagtgtttt ggtcgtattc tggcgagcga
     7441 tgtcgtttcg ccactggatg tcccggggtt tgataactcc gctatggacg gctacgcggt
     7501 gcgtttagcc gatattgcct ccgggcaacc gctgcccgtt gccggtaaat cctttgccgg
     7561 tcagccatac catggtgaat ggcctgcggg tacctgcatt cgtattatga ccggtgcgcc
     7621 ggtgccggaa ggctgcgaag cggtggtgat gcaggagcag actgaacaaa cggacaatgg
     7681 cgtgcgtttt actgctgaag tgcgtagcgg gcaaaatatt cgccgtcgcg gtgaagatat
     7741 ctctgcaggt gcggttgttt tcccggcggg aactcgcctg actaccgcag agctgccagt
     7801 aattgcttca ctggggattg ccgaagttcc ggtgattcgt aaagtgcgtg tagcgctttt
     7861 ttctaccggt gatgaactcc agttgcccgg tcagccgctg ggcgacggcc aaatctacga
     7921 caccaaccgt ctcgccgtac acctgatgtt agaacagctg ggttgcgagg taatcaactt
     7981 agggattatc cgcgacgatc cacatgccct gcgcgccgca tttattgaag ccgacagcca
     8041 ggcggatgta gtgatcagtt ccggcggtgt ttcagtgggt gaagcggatt acaccaaaat
     8101 gattcttgaa gagctggggg agatcgcctt ctggaagctg gcgattaaac caggtaaacc
     8161 gttcgcgttc ggtaaactca gcaatagctg gttctgcggc ctgccgggca acccggtttc
     8221 agcgacgctg accttctatc aactggtaca gcctttgctg gcaaaactaa gcggcaacac
     8281 cgccagcggc ctgcccgcgc gccagcgcgt acgcacagcg tcccgcctga aaaaaacgcc
     8341 aggacgtctt gatttccagc gcggcgtgct gcaacgcaac gccgacggtg aacttgaagt
     8401 gacgaccacc ggacatcagg gttcacatat atttagctcc tttagtctcg gcaactgctt
     8461 tatcgtgctg gaacgcgatc gcggcaatgt ggacgtgcgc gaatgggtgg aagtagaacc
     8521 gtttaacgcg ttgttcggag gcctgtaatg gcggaactca gcgatcagga gatgctgcgc
     8581 tacaaccggc aaatcattct gcgcggtttt gattttgacg gtcaggaggc gctaaaagat
     8641 tctcgtgtgc tggtagtcgg cctcggtgga ctcggctgcg cagcttcgca gtatctggca
     8701 agcgccggtg tcggcaacct gacgctgctc gacttcgaca cggtttcgct ctcgaatctg
     8761 caacgccaga cactgcacag tgatgccacg gtcgggcaac cgaaggtgga atccgcccgt
     8821 gacgccctga cgcggatcaa cccacatatc gcgattacgc cagtcaatgc actgctagat
     8881 gacgcagaac ttgcagcatt gattgctgaa cacgatctgg tgctcgactg tacggataac
     8941 gttgcggtac gtaatcaact gaacgcaggc tgttttgccg cgaaggtgcc gctggtttcc
     9001 ggcgcggcaa ttcgtatgga agggcaaatc acggtcttca cttatcagga cggcgaaccg
     9061 tgctatcgct gccttagccg tttgtttggc gagaacgcgt taacctgcgt agaagcaggc
     9121 gtgatggcac cgttgatagg cgtaattggt tcgttgcagg caatggaagc gatcaaactg
     9181 ctggcaggtt atggcaaacc ggccagcggg aaaatcgtca tgtacgatgc gatgacctgt
     9241 cagtttcgcg aaatgaaact gatgcgcaat ccggggtgtg aggtgtgcgg gcagtaattg
     9301 ccacctccaa gcctgataag acgcgacaag cgtcgcatca ggcatttgct ccagacgcca
     9361 gatgcggcgt gaacgcctta tctggcctac ggttcgggca cagatttcca agcgcggcag
     9421 gcatcggtgc actccgccac gtcatcagca atttatgagg atgcagaacg tccggttaaa
     9481 tcgacgttct gccaaacgct ccctgccagt cctgctcaaa cttcgtcaca gcggcttcca
     9541 ccgccggata gctaatcatc tgttgtgcca catccagtgg cagagtaatt gattcacatc
     9601 cggccagtaa gcagtccagc gcctgacgcg gggttttgaa actcgctgcc agcactttcg
     9661 cctgcggcgc atgcattttc aataactggt gtaagtcggt cacagtctga atgccgctac
     9721 cgccctgagc atcaatacga ttaacgtaag gcgcaacata ttccgcacct gccagcgccg
     9781 acagcagccc ttgtgccgcg ccatataccg cggttcccag cgtcggaatc ccttccgctt
     9841 ttaacatctt aatcgctgcc agcccctcgg ctgtcaccgg aactttcacc acgatatccg
     9901 caataataga acgcagctta agcgcgtcat tcaccatccc ttcagcagtg gtagccatta
     9961 cctgggcaaa cagacgcccc tgaccgccca tcgcttcatg aagttgcgga agcacaacat
    10021 ccagcggttt tttacccgcg gcgataatgc ttgggttagt ggtcacaccc gccagcggaa
    10081 aaatacgtga cagcgccttc accgcaacaa cgtctgaagt atccagatac agttccataa
    10141 ccatcctcaa aatgtacttt ttgtctgaat gtagactatc acgggaaaac tctctgaaca
    10201 gttgacgcac atcaatctaa ctttcattcg aaagtatttt aatgtttgaa cgaaacagag
    10261 aggcaactat gattttcaat attcagcgct actcgaccca tgatggcccc ggtatccgca
    10321 cggtcgtatt tcttaaaggc tgttcgctgg gctgccgctg gtgtcagaac ccggaaagcc
    10381 gcgcccgtac gcaggatctg ctgtatgacg cacgactgtg tctggaaggc tgcgagctgt
    10441 gcgctaaggc cgcgccggaa gtgattgagc gcgcgctgaa tggtttgctt attcatcggg
    10501 aaaagttaac cccggagcat ctgacggcgt taaccgactg ctgtccgaca caggcgttaa
    10561 ccgtgtgtgg tgaagtgaaa agcgttgagg agatcatggc gaccgttctg cgcgataaac
    10621 cgttttacga tcgcagcggc ggcggcttaa cgctttcggg tggtgaaccc ttcatgcagc
    10681 cggaaatggc gacggcgctg ctgcaagcct gccacgaggc aggcattcat actgcggtag
    10741 aaacctgtct gcatgtgccg tggaaatata tcgccccttc tctgccctat atcgatctgt
    10801 ttcttgccga tttaaaacac gttgccgacg cgccgtttaa acagtggacc gacggtaacg
    10861 ccgccagggt gctggataac ctgaaaaaac tcgccgcagc gggcaaaaaa atcattatcc
    10921 gcgtgccgct gattcagggc tttaatgccg acgaaacctc tgtaaaagcc attaccgatt
    10981 ttgccgccga cgagctgcac gttggcgaaa ttcattttct gccctaccac acgctgggca
    11041 tcaacaaata tcacttactt aatctgccct atgacgcccc ggaaaaaccg cttgatgcgc
    11101 cagagctgct cgattttgcc cagcagtatg cctgccagaa agggttaacc gcgaccttac
    11161 gaggataaca accatgacca cactgaaact ggacacgctc agcgaccgca ttaaagcgca
    11221 caaaaatgcg ctggtgcata ttgtgaagcc gccagtctgt accgagcgcg cgcagcacta
    11281 taccgagatg tatcaacaac atctcgataa gccgatcccg gtacgtcgcg cgctggcact
    11341 ggcgcatcac ctggcgaatc gcaccatctg gatcaaacac gatgagttga tcattggcaa
    11401 ccaggcaagc gaagttcgcg ccgcgccgat cttcccggaa tatactgtct cgtggatcga
    11461 aaaagagatt gatgatctgg cagatcgtcc cggtgctggc tttgcggtga gcgaagagaa
    11521 caaacgtgtt ctgcatgaag tgtgcccgtg gtggcgcggt cagacggtac aggatcgctg
    11581 ctatggcatg tttaccgatg agcaaaaagg tctgctggcg accggcatca ttaaagcgga
    11641 aggcaatatg acctccggcg atgcgcacct ggcggtcaat ttcccgctgc tgctggaaaa
    11701 agggcttgat ggtctgcgtg agaaagtagc ggaacgtcgc tcgcgcatca atctgacggt
    11761 gctggaagat ttgcacggcg aacagttcct gaaagcgatt gatatcatgc tggtggcagt
    11821 cagtgaacac attgaacgtt tcgctgccct ggcgcgtgca atggccgcga ccgaaacccg
    11881 cgaaagccgt cgcgatgaac tgctggcgat ggcagaaaac tgcgatctta tcgcccacca
    11941 gccgccgcag actttctggc aggcgctgca actgtgttac ttcatccagt tgattttgca
    12001 gattgaatct aacggtcact cggtgtcgtt tggtcgtatg gatcagtatc tctacccgta
    12061 ctatcgccgc gacgttgaac tgaaccagac gctggatcgc gaacacgcca tcgaattgct
    12121 gcatagctgc tggttgaaat tgctggaagt gaacaaaatc cgctccggct cccactcaaa
    12181 agcctctgcg ggaagtccgc tgtatcagaa cgtcaccatt ggcggacaaa atctggttga
    12241 tggtcaacca atggacgcgg tgaatccact ctcttacgcg atcctcgaat cctgcggtcg
    12301 cctgcgttcc actcagccta acctcagcgt gcgttaccat gcaggaatga gtaacgattt
    12361 cctcgacgcc tgcgtacatg tgatccgctg cggcttcggg atgccggcat tcaacaacga
    12421 cgaaatcgtg atcccggaat ttattaaact cggtattgaa ccgcaggacg cttatgacta
    12481 cgcagcgatt ggttgtatag aaaccgccgt cggtggcaaa tggggctatc gctgtaccgg
    12541 catgagcttt atcaacttcg cccgcgtgat gctggcggcg ctggaaggcg ggcgtgatgc
    12601 caccagcggc aaagtgttcc tgccacaaga aaaagcgttg tcggcaggta acttcaacaa
    12661 cttcgatgaa gtgatggacg cgtgggatac gcaaatccgt tactacacgc gtaaatcaat
    12721 cgaaatcgaa tatgtcgtcg acaccatgct ggaagagaac gtgcacgata ttctctgctc
    12781 ggcgctggtg gatgactgta ttgagcgagc gaaaagtatc aagcaaggcg gcgcgaaata
    12841 tgactgggtt tctggcctgc aggtcggtat tgccaacctc ggcaacagcc tggcggcagt
    12901 gaagaaactg gtgtttgaac agggcgcgat tggtcagcaa cagctggctg ccgcactggc
    12961 ggatgacttc gatggcctga ctcatgagca actgcgtcag cggctgatta acggtgcgcc
    13021 gaagtacggc aacgacgatg ataccgtcga tacgctgctg gctcgcgctt atcagaccta
    13081 tatcgacgaa ctgaaacagt accataaccc acgctacggt cgtggtccgg ttggcggcaa
    13141 ctattacgcg ggtacgtcgt ctatctccgc taacgtaccg tttggcgcgc aaactatggc
    13201 aacgccggac gggcgtaaag cccacacccc gctggcagaa ggcgctagtc cggcctctgg
    13261 tactgaccat ctcggcccta ctgcggtcat tggctcagtg ggtaaactgc ctacggcagc
    13321 gattctcggc ggcgtgttgc tcaaccagaa actgaatccg gcaacgctgg agaacgaatc
    13381 tgacaagcag aaactgatga tcctgctgcg taccttcttt gaagtgcata aaggctggca
    13441 tattcagtac aacatcgttt cccgcgaaac gctgctggag gcgaaaaaac atccggatca
    13501 gtaccgcgat ctggtagtgc gtgtcgcggg ttattccgcc ttcttcaccg cgctctctcc
    13561 agacgctcag gacgatatca tcgcccgtac tgaacatatg ctgtaatctc ttcggcccgg
    13621 ctgcggcgtc gccgggctaa atcattcaca tcatcaattt catccttact ttcattcgaa
    13681 atataatttg tgctctgcgt cacattgtta ttacactgtt acggtttcat tttcgagcca
    13741 ggagcacacc tatgagcgta aaagttatcg tcacagatat ggacggtact tttcttaacg
    13801 acgccaaaac gtacaaccaa ccacgtttta tggcgcaata tcaggaactg aaaaagcgcg
    13861 gcattaagtt cgttgtcgcc agcggtaatc agtattacca gcttatttcg ttctttcctg
    13921 agctaaagga tgagatctct tttgtcgcgg aaaacggcgc actggtttac gaacatggca
    13981 agcaactgtt ccacggcgaa ctgacccgac atgaatcgcg gattgttatt ggcgagttgc
    14041 taaaagataa gcaactcaat tttgtcgcct gcggtctgca aagtgcatat gtcagcgaaa
    14101 atgcccccga agcatttgtc gcactgatgg caaaacacta ccatcgcctg aaacctgtaa
    14161 aagattatca ggagattgac gacgtactgt tcaagttttc gctcaacctg ccggatgaac
    14221 aaatcccgtt agtgatcgac aaactacaca tagcgctgga tggcattatg aaacccgtca
    14281 ccagtggttt tggctttatc gacctgatta ttcccggtct acataaagca aacggtattt
    14341 cgcggttact gaaacgctgg gatctgtcac cgcaaaatgt ggtagcgatt ggcgacagcg
    14401 gtaacgatgc ggagatgctg aaaatggcgc gttattcctt tgcgatgggc aatgctgcgg
    14461 aaaacattaa acaaatcgcc cgttacgcta ccgatgataa taatcatgaa ggcgcgctga
    14521 atgtgattca ggctgtgctg gataacacat ccccttttaa cagctgacct ctctaccgga
    14581 gccatgcgct ccggttttcc cttcttttta catcctgtac atcacatcaa atcattaaac
    14641 ttgcattaac ttatttataa cttgtattgt cacatgcaaa ttagcatagt ttcgaatgaa
    14701 agttaatgca aggtggttat ggcttctact tttaccagcg acacattgcc tgccgatcac
    14761 aaagcagcta tccgtcagat gaagcacgcg ctgcgggcgc agcttggcga tgtccagcag
    14821 atctttaatc agctaagcga tgacattgcc acgcgagtgg ctgaaatcaa cgcactcaaa
    14881 gcacagggcg atgccgtctg gccggtgctg tcttatgccg atatcaaagc aggtcatgtt
    14941 actgcagagc agcgcgaaca gattaaacgt cgcggttgtg cggtgataaa aggccatttc
    15001 ccccgcgaac aagcgctagg ctgggatcag tcgatgctgg actatctgaa ccgcaaccgc
    15061 tttgacgagg tctacaaagg ccccggcgat aatttcttcg ggacgctcag cgcttcacgt
    15121 cccgagattt accccatcta ctggtcgcag gcgcaaatgc aggcccgcca gagtgaagaa
    15181 atggcgaatg cgcagtcgtt tctcaatcgt ctgtggacat ttgaaagtga tggaaagcaa
    15241 tggtttaacc cggatgtgag cgtcatctac cctgaccgta tccgccgcct tccgcccgga
    15301 acgacctcca aaggtcttgg agcgcatacc gactccgggg cgctggaacg ctggctgctt
    15361 ccagcgtatc agcacgtttt cgccaacgtc tttaatggca atctggcgaa atacgatccc
    15421 tggcatgcgg cacatcgtac ggaagttgaa gagtacacgg tggacaacac caccaaatgt
    15481 tccgtgtttc ggacattcca gggctggact gcgctctcgg atatgctgcc tggtcagggg
    15541 ttgctgcacg tcgtgcccat tcctgaagcc atggcgtacg tactgttacg tccgctgctt
    15601 gatgatgtgc cggaggatga actgtgcggc gtagcgcccg gaagagtgtt gccggtatca
    15661 gagcaatggc atccactgtt aattgaggcg ttaaccagca ttccaaaact cgaggccgga
    15721 gactccgtct ggtggcactg cgacgtcatc cattccgttg cccccgttga aaatcaacag
    15781 ggttggggca acgtgatgta cattcctgcg gcaccgatgt gcgagaaaaa tcttgcctac
    15841 gcgcacaagg tgaaggccgc actggaaaaa ggcgcatcgc cgggcgactt cccgcgcgag
    15901 gactatgaaa caaactggga aggacgcttt acgcttgccg acctcaacat tcacggtaag
    15961 cgagcgttgg gcatggatgt ttgagagtta atggaagtga attgatgtgg gattaagaaa
    16021 atcgattcga cgttactcag attgctgaca agtgcgcttt cttaatgcct gatgcggcgt
    16081 taacgcctta tccggcctac aaagtcctgc agattcaata tattacaggt actgcgtagg
    16141 cctgataagc gtagcgcatc aggctatttt gcatttgtca tcagccgtcg tcaccccgac
    16201 agtatcgggg tgacggataa tcacttactc gatcccttta ctacgcaggt aatcttcgta
    16261 attaccgcta aagtcgatca cgcgttccgg ggtgatttcc agaatgcggg tcgccaggga
    16321 gcttacgaac tcacggtcgt gtgaaacaaa gatcagcgtg ccctgataca gttccagcgc
    16381 catgttcagt gactcgatgg attccatatc caggtggttg gtcggttcgt ccatgatcag
    16441 aatgttcggc ttctgcatca ttaacttacc aaacagcatc cgcccttttt ccccaccgga
    16501 aagcacttta gctggctttt tgatgtcgtc ctggctgaac agcaaacgac cgagaatgct
    16561 gcgtaccgcc tgctcgtcat cgccttcctg cttccactga ctcatccatt cgaacacggt
    16621 cagatcattt tcaaactcat attcgtggtc ctgggcgtag taaccaatgc gcgcgttttc
    16681 agaccatttt acggtgccgc tgtccggttg cagatcgccc accagcgttt tcagcagcgt
    16741 tgatttaccg acgccgttgg tacccagtac cgcaagtttt tcaccgacct ccagcagcag
    16801 gttgagattt ttaaacagtg gaccgttatc aaaccctttg gtcagacctt ccacttccag
    16861 cgcgttacgg aacagtttct tatcctgttc aaaacggata aacgggttct gacggctgga
    16921 ggctttcacc tcttccagtt tgattttatc aatctggcgc gcgcgcgaag ttgcctggcg
    16981 agatttcgag gcgttggcgc taaagcggct aacgaaagat tgcaactcag caatctgcgc
    17041 tttcttcttg gcgttatcgg ccagcagacg ttcacgcgct tgggtcgccg ccgtcatgta
    17101 ctcatcgtag ttacccggat aaacgcgcag ctcgccgtaa tccagatccg ccatgtgggt
    17161 acagaccatg ttaaggaagt gacggtcgtg cgagatgatg atcatggtgc tgtcacgctc
    17221 gttcagcacc tgttccagcc agcgaatggt gtcgatgtcg aggttgttgg tcggttcgtc
    17281 gagcaggaga atatccggct cagcaaacag cgcctgcgcc agaagcacac gcagcttcca
    17341 gccaggagca acttcactca tcgggccgta gtgctgttcc actggaattc ccacgccaag
    17401 caacagttca ccggcgcgag cttccgcaga gtaaccgtcc atttcgccgt atttcacttc
    17461 cagatcggcc actttatagc cgtcttcttc actcatttcc ggcaaagcat agatgcggtc
    17521 gcgctcctgc ttcacttccc acaactcttt atgccccatg atcaccgtat ccagcacagt
    17581 gaactcttca aaggcaaact gatcctgacg cagcttacca atgcgctcgt tgggatcgag
    17641 ggaaacgtta cccagcgtcg gctcaaggtc gccgccgaga atcttcataa aggtggattt
    17701 accactaccg ttcgcgccaa tcaggccgta acggttgccg ccgccgaatt tgacggaaat
    17761 gttttcaaac aacggcttac tgccgaactg catggtgacg ttactggaaa ctaacacagg
    17821 cgtatcctga aaagagatat gacaaaccgc gcattatgcc ataagtctga aagaagatcc
    17881 tgctcctcgc ttaaggtcta cactcaagcc aaaggcaaaa aatgtgattt cgtacacatc
    17941 tgatttcact gtgagctgga atgaacttat aatgcgcttc caatactcta atattctcaa
    18001 cccaatggcc tgccaggcac aaaatctcgc ttaacatgaa tatgaaattg aaaacattat
    18061 tcgcagcggc cttcgctgtt gtcggctttt gcagtaccgc ctctgcggta acttatcctc
    18121 tgccaaccga cgggagtcgc ctggttggtc agaatcaggt gatcaccatt cctgaaggta
    18181 acactcagcc gctggagtat tttgccgcgg agtaccagat ggggctttcc aatatgatgg
    18241 aagcgaaccc gggtgtggat accttcctgc cgaaaggcgg tactgtactg aacattccgc
    18301 agcagctgat cctgccggat accgttcatg aaggcatcgt cattaacagt gcagagatgc
    18361 gtctgtatta ctatccgaaa gggaccaaca ccgttatcgt gctgccgatc ggcattggtc
    18421 agttaggcaa agatacgcct atcaactgga ccaccaaagt tgagcataag aaagcaggcc
    18481 cgacctggac gccgaccgcc aaaatgcacg cagagtaccg cgctgcgggc gaaccgctac
    18541 cggctgtcgt tccggcaggt ccggataacc cgatgggtct gtatgcactc tacatcggtc
    18601 gcctgtatgc tatccatggc accaacgcca acttcggtat cggcctgcgt gtaagtcatg
    18661 gttgtgtgcg tctgcgtaac gaagacatca aattcctgtt cgagaaagta ccggtcggta
    18721 cccgcgtaca gtttattgat gagccggtaa aagcgaccac cgagccagac ggcagccgtt
    18781 atattgaagt ccataacccg ctgtctacca ccgaagccca gtttgaaggt caggaaatcg
    18841 tgccaattac cctgacgaag agcgtgcaga cagtgaccgg tcagccagat gttgaccagg
    18901 ttgttcttga tgaagcgatc aaaaatcgtt ccgggatgcc ggttcgcctg aattaatcgt
    18961 caaaacgtaa aacaaaggcg gactcataat ccgccttttt tatttgccag accttaccag
    19021 gccgggatca taactaacaa aacgtatccc accagcgccg cccacaacag catcggtatt
    19081 gaatagagat ggaaacgcca ccagatgcgg cgatcgttcg ccatacgtag cgcaatcaaa
    19141 tttgccagcg atcctggtaa taaaccaaag ccccccacgt ttaccgccca tgccagtaat
    19201 aaagacggag gtacataatt gagcagcaaa atggtactcg gcacgttact gatcacctgc
    19261 gataaaccga ctgccgttaa ccataacccg ggttcagata aatgactcac gttacccaac
    19321 acgccttgca acgctggaag ctgggtcagt aaatggacgt cgataaacat cgccataaac
    19381 accagcagca acgtccagtc cacactcaac accacgcgac gcgccagcag cacaaagccc
    19441 gccgccacaa tcaccagtcc ccacagctct tgtttgaact ccagcgccgt cagaaagacg
    19501 atatacagcc ccagacaatt ccacaccagc cgcggtttcc actccggtgt ttgcaccccc
    19561 gtatggtatt gcagcgcctt tccagggaaa caacaccagc acaacagcag gagtgtcagc
    19621 atcattgcgc cagccatcgg tgccatttgg gcaataaatc cggcaaacga aagaccagaa
    19681 cgtccccaga taagaatatt ttgcgggttg ccaattggcg tcagtagcga accagcgttg
    19741 actgccagcg cctcaaaaat aatcagccga ttaaccggga tctcacacag tctttttagc
    19801 gtgatagtca gcggaacaac aataaacagc gcgacatcgt tggtcagaaa ggtagaaagc
    19861 agcgccgccg ccagcaccat aaacatcgcc agccgacgct ccgtagcaaa gcggcgcacc
    19921 attttgcgcc ccagcacatc aaaataaccg cttaactcca cacctttggt cagcagcatc
    19981 aggccgctta aggtgatgat ggtgtgccag tcgatagcag caggccagga tttcggtgca
    20041 aagggcacga aaaagcttaa tccgatacca acaagaatta ataactgaaa aaaacgatcg
    20101 ccttgcagcg tgcgtaaaaa aggcaggctc atttggcacc gtgtttctgg gtaaacaaac
    20161 gaaaagcgtc gagcgtctct tcactaacat ggtgctccat gccttccgcg tcgcgacggg
    20221 cgatttccgg actgacgccc aacaccaaca agaaattttc gactatctga tgtcgctcgc
    20281 ggctttcctg cgccagcttc tctccttctg ccgttaaaaa cacgccacgc caggggatca
    20341 tttcaatcag ccccatggtt gccagccgct taagcatttt agccaccgtc ggttgcgaaa
    20401 ctcccagacg agcagccatg tccacctgac gagcttcccc cacttccctg atcaagtcag
    20461 aaatcagctc aacgtaatca tcaataagct cgcgccgatg cgcctctctg acctggcgga
    20521 acccttcaac gtgctcttcc acgttcacta actgcgtcac tttttttgct attggcgtac
    20581 ctgcgcgacg actcattgtg cttcctcatt atggtgacgc atacatagcg ttctcatttc
    20641 gtgcgcacat tgtaaaccag agttgcgaag gtacaaaaaa ttaacgtttt agcaatagct
    20701 atataatata gcctgtgcta tatctgtatg taatgcaatc atccctcaag gatcgacggg
    20761 attagcaagt caggaggtct tatgaatgag ttcaagaggt gtatgcgcgt gtttagtcat
    20821 tctcccttta aagtacggtt aatgctgctc tctatgttgt gcgatatggt caacaacaaa
    20881 ccgcagcaag ataaaccttc agataaatag cggcgtcgcg gtacgccgct tcactcctgc
    20941 ttttatgcag gcataacgcg ttttggtctg aaaaaaacca ctttttgtcg gatttgcaat
    21001 ccccttcgca aaagatttgt tcgtcagtag ttgacctgaa cggcggctcg ctctatcttc
    21061 ttgcagccct gcgtatattg cggctcgcgg atgcggaccc ctttcccctc ttcacgcact
    21121 cttgcaggta ttgacccttg acgccagggt aagcacatgg cgtttgttac gatagtggca
    21181 tatgaattta accctcaaag aatcgcttgt tacccgtagc cgggtattta gcccgtggac
    21241 tgcgttctac tttttacagt cgctattaat taacctcggc ttaggttacc ccttcagttt
    21301 gctctacacc gctgcgttta cggctatttt gcttttgcta tggcgaacat tgcctcgcgt
    21361 acaaaaagtt ctggtcggtg tcagttcgct ggtggcggct tgttatttcc cttttgctca
    21421 ggcctacggc gcgcctaatt tcaatacatt gctggcattg cactccacca atatggaaga
    21481 gtcgaccgaa atcctgacga tttttccgtg gtacagctac ctggtcggct tatttatttt
    21541 tgcgctcggc gtaatagcaa tcaggcgaaa aaaagagaat gaaaaagcgc gctggaatac
    21601 cttcgacagc ctgtgtctgg tattcagtgt ggcgacattt tttgttgctc ccgtgcaaaa
    21661 cctggcctgg ggtggcgtat ttaaactgaa agatactggc tatccggtat ttcgttttgc
    21721 taaggatgtc atcgtcaata ataacgaggt gattgaagag caagaacgga tggcaaaact
    21781 ttccggaatg aaagatacct ggacggtcac tgccgttaag ccgaagtatc agacctatgt
    21841 ggtagtgatc ggtgaaagcg cgcgtcgcga tgccctcggt gcctttggcg gtcactggga
    21901 caataccccg tttgccagca gcgttaacgg tttgatattt gctgactaca ttgctgccag
    21961 tggctccacg cagaaatcgc ttggcttaac gctcaatcgc gtagtcgatg gcaaaccaca
    22021 gtttcaggat aactttgtca ccctggcaaa tcgcgcgggc ttccagacct ggtggttttc
    22081 caaccagggc caaatcggcg aatacgatac tgctatcgcc agcatcgcca aacgtgcaga
    22141 tgaagtgtac ttcctgaaag aaggtaattt tgaagcagat aaaaacacga aagacgaagc
    22201 gttactggat atgaccgctc aagtgctggc gcaagagcac tcgcaaccgc agctgattgt
    22261 tctgcatctg atgggctcgc atccgcaggc ctgcgacagg acacaaggca aatacgaaac
    22321 ctttgtgcaa tcgaaagaaa cgtcgtgcta tctctatacc atgacgcaaa cggacgattt
    22381 actgcgcaag ctgtacgatc agttacgcaa cagcggcagc agcttctctc tggtttactt
    22441 ttctgaccac ggtctggcct ttaaagagcg cggcaaagac gtgcaatacc ttgcccatga
    22501 tgataaatat cagcaaaatt tccaggtgcc ttttatggtc atttccagcg gcgataaagc
    22561 gcatcgggtg attaaagccc gccgctcagc caatgacttc ttaggctttt tctcgcagtg
    22621 gacgggaatt aaagcgaagg aaataaatat caaatacccg tttatatctg agaagaaagc
    22681 cgggccgata tacatcacca acttccagtt acagaaggtg gattacaacc atctcggaac
    22741 cgatattttc gacccgaaac cttaaaaaca aaaatccgcc ccgagaggcg gatttttata
    22801 tcaccaaagt gattagaagc ggtaaccaac accggcaatc caggtgccta cgtcaacgct
    22861 acgaatacgg ctctgctcgt aagagaagtc cagagcaacg ttttccatcg ggttgaactg
    22921 cagaccagca ccgtaggaga aaccgtagtc gctggtgtcg tgtttgtagg tcgggtattc
    22981 agtggtctgg aatttaccat aacccacacc cactacaccg tagatgcttg cccagtcgtt
    23041 aatgcggtaa gccggaccag cagtgatgcc gtagtactgg tttttgttgt agtcaccaga
    23101 gcttgcagta cggcttttct cggtgtaagt gaaagaaccg atcacaccca gcgggctgtt
    23161 gtcttcttca tagcggtatt tcaggttgaa accgcccatt ttgttcattt ggccctgagc
    23221 gtcgctctgt gcgtaaccgc cagttacggt agaagtcgca gctacggaag tacctgcggt
    23281 gaaagccaga actgcggcca gtgctgaaag acatgcaatt tttttcataa ccacctcaaa
    23341 tgtgattcaa ataagtccta agttttaaat atatcaaaaa ttaatgggaa actcttcgcg
    23401 atttgtgatg tctaacgggc catttcatgt aacagaacgt ttccatacac cgctatccat
    23461 ctaaatttaa atcacttttt cagagaactg cgtaagtatt acgcatgttt tccctgtcat
    23521 tcatccagat tattcctaat caccagacta atgattccat caatcctggc gcattttagt
    23581 caaaacgggg gaaaattttt tcaacaaatg ctcaaccagc attgggtata tccagtacac
    23641 tccacgcttt acttaagtct agatatttgt gggagaaagg atgcctggtt cattacgtaa
    23701 aatgccggtc tggttaccaa tagtcatatt gctcgttgcc atggcgtcta ttcagggtgg
    23761 agcctcgtta gctaagtcac tttttcctct ggtgagcgca ccgggtgtca ctgcgctgcg
    23821 tctggcatta ggaacgctga tcctcatcgc gttctttaag ccatggcgac tgcgctttgc
    23881 caaagagcaa cggttaccgc tgttgtttta cggcgtttcg ctgggtggga tgaattatct
    23941 tttttatctt tctattcaga cagtaccgct gggtattgcg gtggcgctgg agttcaccgg
    24001 accactggcg gtggcgctgt tctcttctcg tcgcccggta gatttcgtct gggttgtgct
    24061 ggcggttctt ggtctgtggt tcctgctacc gctggggcaa gacgtttccc atgtcgattt
    24121 aaccggctgt gcgctggcac tgggggccgg ggcttgttgg gctatttaca ttttaagtgg
    24181 gcaacgcgca ggagcggaac atggccctgc gacggtagca attggttcgt tgattgcagc
    24241 gttaattttc gtgccaattg gagcgcttca ggctggtgaa gcactctggc actggtcggt
    24301 tattccattg ggtctggctg tcgttattct ctcgaccgct ctgccttatt cgctggaaat
    24361 gattgccctc acccgtttgc caacacggac atttggtacg ctgatgagca tggaaccggc
    24421 gctggctgcc gtttccggga tgattttcct cggagaaaca ctgacaccca tacagctgct
    24481 ggcgctcggc gctatcat
//
LOCUS       DAZSIO010000047        24184 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZSIO010000047 DAZSIO010000000
VERSION     DAZSIO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.047"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(25..597)
                     /locus_tag="ACHX5T_001740"
     CDS             complement(25..597)
                     /locus_tag="ACHX5T_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR8466975.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            815..1675
                     /gene="tesB"
                     /locus_tag="ACHX5T_001741"
     CDS             815..1675
                     /gene="tesB"
                     /locus_tag="ACHX5T_001741"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR8466976.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="ACHX5T_001742"
     CDS             complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="ACHX5T_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR8466977.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="ACHX5T_001743"
     CDS             complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="ACHX5T_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR8466978.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3559..5340)
                     /locus_tag="ACHX5T_001744"
     CDS             complement(3559..5340)
                     /locus_tag="ACHX5T_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR8466979.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5333..7105)
                     /locus_tag="ACHX5T_001745"
     CDS             complement(5333..7105)
                     /locus_tag="ACHX5T_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR8466980.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7135..7593)
                     /gene="decR"
                     /locus_tag="ACHX5T_001746"
     CDS             complement(7135..7593)
                     /gene="decR"
                     /locus_tag="ACHX5T_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFR8466981.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7747..8565)
                     /gene="cof"
                     /locus_tag="ACHX5T_001747"
     CDS             complement(7747..8565)
                     /gene="cof"
                     /locus_tag="ACHX5T_001747"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR8466982.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8665..10357
                     /locus_tag="ACHX5T_001748"
                     /pseudo
     CDS             8665..10357
                     /locus_tag="ACHX5T_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10422..11117
                     /gene="queC"
                     /locus_tag="ACHX5T_001749"
     CDS             10422..11117
                     /gene="queC"
                     /locus_tag="ACHX5T_001749"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR8466983.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11169..11567)
                     /gene="fadM"
                     /locus_tag="ACHX5T_001750"
     CDS             complement(11169..11567)
                     /gene="fadM"
                     /locus_tag="ACHX5T_001750"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR8466984.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11661..12032)
                     /locus_tag="ACHX5T_001751"
     CDS             complement(11661..12032)
                     /locus_tag="ACHX5T_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR8466985.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12183..14054)
                     /gene="ppiD"
                     /locus_tag="ACHX5T_001752"
     CDS             complement(12183..14054)
                     /gene="ppiD"
                     /locus_tag="ACHX5T_001752"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR8466986.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DVASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14246..14536)
                     /gene="hupB"
                     /locus_tag="ACHX5T_001753"
     CDS             complement(14246..14536)
                     /gene="hupB"
                     /locus_tag="ACHX5T_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR8466987.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14727..17081)
                     /gene="lon"
                     /locus_tag="ACHX5T_001754"
     CDS             complement(14727..17081)
                     /gene="lon"
                     /locus_tag="ACHX5T_001754"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR8466988.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17269..18543)
                     /gene="clpX"
                     /locus_tag="ACHX5T_001755"
     CDS             complement(17269..18543)
                     /gene="clpX"
                     /locus_tag="ACHX5T_001755"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR8466989.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18669..19292)
                     /gene="clpP"
                     /locus_tag="ACHX5T_001756"
     CDS             complement(18669..19292)
                     /gene="clpP"
                     /locus_tag="ACHX5T_001756"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR8466990.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19539..20837)
                     /gene="tig"
                     /locus_tag="ACHX5T_001757"
     CDS             complement(19539..20837)
                     /gene="tig"
                     /locus_tag="ACHX5T_001757"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR8466991.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21181..21498)
                     /gene="bolA"
                     /locus_tag="ACHX5T_001758"
     CDS             complement(21181..21498)
                     /gene="bolA"
                     /locus_tag="ACHX5T_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR8466992.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21803..22381
                     /locus_tag="ACHX5T_001759"
     CDS             21803..22381
                     /locus_tag="ACHX5T_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR8466993.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22425..23900
                     /gene="ampG"
                     /locus_tag="ACHX5T_001760"
     CDS             22425..23900
                     /gene="ampG"
                     /locus_tag="ACHX5T_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR8466994.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5667 a         6666 c         5827 g         6024 t
ORIGIN      
        1 ccgggcggag agggttaacg tcgcttaata ttgcgtagga gcgggaactg ccgaagacgg
       61 attcacctga gttggtgaag tcgaaggtac ggtagtcgtt gcgccaccgc tggcctgaac
      121 cggcacggcg gtttgctgta ccggcaccaa tgtcaggtcg gctttagttc cgccctggtt
      181 gatcaccggc tgaacggtat cggtgataaa taccagtttg tcattcacgg taatcgccgc
      241 actcaacaga atacgcgcgt tcggctgaac atctgccggg ttaaatggca gaacaaagct
      301 gaatggtgac tgtttacctt cagtacgcac cgctttctgc gccagcactt ttgacggtgc
      361 atcggctaac gacgcgtcag aaagtgtcac ggtcagcaca gcatcaggcg gcagtgcgac
      421 tttctgacgg atccagacag taccggagac attcggttgc tggatagcag gttgttgtgt
      481 tgctgaaata gacgtatttg cagccggggc tggcgtctga atatccgcgc ttttatctgc
      541 gcaagccgcc aacgcaatcg caaccgctaa accactggcc atgtgcacga gtttcattca
      601 tttctcctta ttatcaatgc accagcgggc taactttcct cgccggaaga gtggttaaca
      661 aaatagtaac gtcaacaagt gtggcacaca tcacgcattt ctgcctgtaa ttagcccgta
      721 attcagacta tttcacccat cggaccagtg gcaaatttgc gttatactca actcactttg
      781 gcttgctgcg gcagctttgt tactggagag ttatatgagt caggcgctaa aaaatttact
      841 gacattgtta aatctggaaa aaattgagga aggactcttt cgcggccaga gtgaagattt
      901 aggtttacgc caggtgtttg gcggccaggt cgtgggtcag gccttgtatg ctgcaaaaga
      961 gaccgtcccg gaagagcggc tggtacattc gtttcacagc tactttcttc gcccaggcga
     1021 tagtaagaaa ccgattattt atgatgtcga aacgctgcgt gacggtaaca gcttcagcgc
     1081 ccgccgggtt gctgctattc aaaacggcaa accgattttt tatatgacgg cctctttcca
     1141 ggcaccagaa gcgggtttcg aacatcaaaa aacaatgccg tccgcgccag cgcctgatgg
     1201 cctgccttcg gaaacgcaaa tcgcccaatc gctggcgcac ctgctgccgc cagtgcttaa
     1261 agataaattc atctgcgatc gtccgctgga agtccgtccg gtggagtttc ataacccact
     1321 gaaaggtcac gtcgcagaac cacatcgtca ggtgtggatc cgcgcaaatg gtagcgtgcc
     1381 ggatgacctg cgcgttcatc agtatctgct cggttacgct tctgatctta acttcctgcc
     1441 ggtagctcta cagccgcact gcatcggttt tctcgaaccg gggattcaga ttgccaccat
     1501 tgaccattcc atgtggttcc atcgcccgtt taatttgaat gaatggctgc tgtatagcgt
     1561 ggagagcacc tcggcgtcca gcgcacgcgg ctttgtgcgc ggtgagtttt atacccaaga
     1621 cggcgtgctg gttgcctcga ccgttcagga aggggtaatg cgtaatcaca attaaaaaaa
     1681 tagccggagg tgaaaaccgt ccggctattt tttgcagtgc ttgttacgcg ttataggcat
     1741 tctcgccgtg gctgttgacg tccagccctt ctcgctcttg ttcttccggc acacgtaggc
     1801 caaccgtcag atccgccagt ttgtagccaa taaatgccac cacaccggac cagacgatcg
     1861 taatggcgat gctttccaac tgtaccagca actgatggcc catcgtcaca ccttcagcga
     1921 agcccacgcc gcccagagaa ctggcggcaa aaatcccggt catgatacag ccgacaatgc
     1981 cacaaacgcc gtgcacaccg aagacatcgc agggatcatc cacccgcagc aagcgtttga
     2041 gcatggtaac gccccacaaa cccgccagac cagctaccac gccgataatc aacgcgccgc
     2101 caaccccaat gtagccgcac gctggcgtca cgccgaccag accggcaatc gcgccagaac
     2161 acgctcccag cagagaaggc ttaccacgca gcgcccattc accgaagatc cagccaagaa
     2221 ttgccgccgc cgttgcgacc acagtattca caaatgccag tgccgcgatt tcattcgccg
     2281 tgcccactga cccggcgtta aagccaaacc aaccgatata gagaatggca gtcccggtga
     2341 agaccatcgg caggttgtgc ggtttaaacg cctctttacc gaagcctacg cgtttgccta
     2401 tcagatacgc gcccaccaga ccggcgattg ctgcgttaat gtgcaccacc gtgccacccg
     2461 cgaaatccag cgcaccgtga gaagccagca aaccaccgcc ccacaccata tgcgcaatcg
     2521 gaatgtaaga gagcgtcagc cataccacca cgaaaatcaa cacagctgag aagcggattc
     2581 gttccgccag cgccccaact atcaagccta cggtaatgca ggcaaacgat ccctgaaacg
     2641 ctacgtggat atactgataa atgctgccca tcaccgccgt cagttcgatg ttttttagca
     2701 tcaaccagtt aatgttgccg aagaagttgt tgccctcacc aaacgccagc gaataaccgt
     2761 aaaccaccca gagaatacag accagtgcaa acgtcaccgt cacctgcgtc agcatcgaca
     2821 gcacgttttt gccgcgaatc aacccaccgt aaaacagggc aatccccgga atagtcataa
     2881 acagcaccag cgcagtacaa atcatcataa acgcattgtc ggctttatcg gccaccgcag
     2941 gtgcagccat taccagtccc ggcagcatcg ccagtgaagc aagcccagtt tttatcgtcg
     3001 ctatcttcat ttttcgttcc tgttgctgtg tgccagagat tacagcgccg cttcgtcggc
     3061 ttcgccggta cgaatacgaa tgacgcgttg caattcagcg acgaagattt tgccgtcgcc
     3121 aatttttccg gtgtaagccg ccttactgac gatatcgatc acttcatcga gttggtcatc
     3181 agcaatcgcc acatcaattt ttacttttgg caggaagttg acgctgtatt ccgccccccg
     3241 gtacagctcg gcatgccctt tctgacgccc gaaacctttc acttcggtga cggtcaggcc
     3301 ctgaatacca atggaagata acgcttcacg aacgtcttcc agcttgaatg gtttgattat
     3361 cacggtcacc agcttcatag atcccctccg gtcagaattc ggtaatggtt ctgctacacg
     3421 aagaaggtat tgcaagcggt gtgccagaaa tgaaaaacga gaaagagagc aggaagttaa
     3481 gaagattgaa aatgaaaacg cactatgaca gtgcacagtg cgttacattt ttgcaccaac
     3541 gatgaaatat tgcgctattc aggcgctcaa tgattcctct tcacgcacgc tggctgccag
     3601 ctcttcgccc gcaagttgca gttgatacat ctgccagtag cgtccctggg ccgccagcag
     3661 ttgctggtga gtgccctgct ccacggcttg cccacgatga agcaccagaa tggtgtcggc
     3721 atcaataata gttgataagc ggtgagcaat caccaccagc gtggtatgtt cacgcaccgc
     3781 cgccagagca tgttgaatcg cctgttcagt accggagtca atgctggcgg ttgcctcatc
     3841 aaggatcagg atttgcggcg tctccaccag cacgcgcgcc agtgccagca gttgcttttg
     3901 cccgactgag agattatttc cctgctcgcc cagcggcgta taaatacaat cactcatgct
     3961 gcgcgccagc tccgccagtt gcacggtttc cagcgcctgc cagacgcgtt cttcggagat
     4021 atcccgcccc agcgtcacat tagcgaggaa ggtatccgcc agcaccaccg gatcttgctg
     4081 caccattgcc acgccctggc gcagcgcgct atgacttagc gaacttaatg gacgaccatc
     4141 aaggtgaatc tcaccttccg ttagcgggta atagcccatc aataaactgg cgagggtact
     4201 tttaccactg ccggtatgcc cgaccagcgc cacaaagtta cgcgaaggca cagagagatt
     4261 aatgttcttt agcaccagat tgtcatcgcg ataagcaaat gacacgttat cgacttcgat
     4321 ggtgccactc tgtaacgggt aatcatcatt gccatattgc tggcgcggtc cgtccatcag
     4381 ttcaaacacg cgctcaccag caacaacagc ctgttgcagc atcgcctgtt gcgtggtcag
     4441 ttcgattaag ggttcgttaa gtcgcccaag atagctgata aacgcataca acacgcccac
     4501 ttcaatggtg ccgctggcgg agaagccaaa cagcatcaat aagccacaaa gaatgagcga
     4561 tgaaaacaga ctcagcagcg gacgcagcag aaaaccgtcg aggcgcaggg tttgcatcct
     4621 agccatatag tgtgaacgac tggcctcccc catacgttcg ccaaatcgcg cctgctgacg
     4681 aaactgctgg ataacgctca tgccattgat gatttcgtta aagccgtcgt tgatatccgc
     4741 cagataggcg cgcacacggc ggacaatcgg cgtgctgtaa cgctggtata tcaccattac
     4801 caccagcacc accgggaaaa tcattatcgc caccagcgcc attcgccagt cgaggctgaa
     4861 catcgccacc agcatcgcgc ccaccagtgc ggcactgcgc aggacagttg ccactacggt
     4921 aacgtagaga tcgcggatca cttcagtgtc attagtgacg cgggaaatca cctgcccgac
     4981 gggttgggta tcaaactcgc ttaatggctg gcgtaacgca gcatccatca cgtcggtacg
     5041 caactgttgc actgcgccta ctgccgcccg attaaacagc agcgactgcg cgtaatgtag
     5101 cccggcggca aacagttgta gcccaacata cgccgcagcc agccctgcaa ccactttcaa
     5161 cggcaggtta tttttcgcta ccatattgtc gataaaatag ctgataagca gcggcccact
     5221 gacttctgcc gccgccgcaa cccacatcat caggaccgca atccccagcg gtttacgcca
     5281 cggcgaacca tacgctaaca ggcgcttgag agtcggccac agttggctaa aactacgcat
     5341 cgacggcctc ctcgcgaatt tccggaacgt cgtcgagcgc cgcctccagt tgttgatagc
     5401 gatacatatc gcgataccaa ccgctttgtt gcgccagctc atcatgattg ccacgctggg
     5461 cgatatgtcc gtgctgcatc acaataattt cactggcttc cgtcagtgcc gaaaggcgat
     5521 gggcactgat gattaccgtt cttccctgtc cccactgacg caggttatgc aggatctggt
     5581 gctctgtgcg tccgtccacc gccgaaagcg catcatcaag gatgaggatt tccgcgttga
     5641 ctaataacgc tcgagcaatg gagatacgct gtttttgccc accggaaagc atcacgccgc
     5701 gctcgcccac ttctgtatcg taaccttgcg gtagacgcaa aatatcgtca tgtacgctgg
     5761 ctaaccgcgc gacatgctca atctcttgct gggtggcatt cgggcaaccc agcgcgatgt
     5821 tattcgccac ggtgtcagaa aaaaggaatg gcgtctggct gaccaccgcc aggcggctac
     5881 gccagctatc gagttgtaac ttcgtcagag gaatatcatg aaagcgaata tccccctcgc
     5941 tgacgtcgaa atgacgctga atgagcgaca acagggtact tttgccggaa ccagtcggcc
     6001 cgcaaatacc cagcatctga ccgggtttca gggcgaaatt gacgttttcc agcgcaggat
     6061 ggtcagtctg cggatacgtg aactggtgaa tatttacatc cagttcgcca cgcccttccg
     6121 gcacgggttc gctaccatcg ttcaccaccg gcgcttccgc cagcatggcg cgaatacggc
     6181 tgtatgcagc actaccacgt tccacaatgt taaacatcca tgccagcgcc agcattggcc
     6241 aaatcatcag acctaaatac atcataaaac tggtgagctg gcccagcgtt aaactgccct
     6301 gcaccaccat ccaactaccg ccgccaatcg ccagcaagtt cgccatacca atcgcgatat
     6361 agatggtcgg gtcgaagcga gcatcaatac gcgccacccg catgtttttt ttgccggtat
     6421 cttcggcatc cgcggcaaat aacgccgact ggcgatcttc cagaccaaag gctttgatca
     6481 tgcggatact ggtgaggctt tcctgggtgc ggtcattaag actggaaaac gccgcctgtg
     6541 ccagcttaaa gcgttcatgc aaggcatcgc cgttgcgctt aatcatgatc gccatcactg
     6601 gcatcggtaa cagggcaaat aaggtcaact gccagctaat ttgcgtagac atcataatca
     6661 acacagcgca gcccattacc agtgaatcca ccagcgtcag caccccttct ccggcggcaa
     6721 acacgacgcg atcgacgtca ttggtcgcac gagccatgag atcacccgtg cgatgacgca
     6781 ggtaaaactc cgggtgctgc cggcttagct gacggtaata atcttcacgc agttcaacag
     6841 ccagttggta agacgcgcca aacagcaata cgcgccagac ataacgcagg agataaacca
     6901 caacggcaat cagcaccatg gtggcgatcc acatcaggct cagcccggta gtaaagtgtt
     6961 gttctgtcac gccatcgaca acaataccaa ccacttttgg cggaaccagt tgcagcatcg
     7021 cgataatgac aagcaaggcg acagccccaa gatagcgacg ccattcccga cggaaatacc
     7081 agcttaattg agcaaataat cgcacgcgtt gtgtcctgac ctgattctgg atatttattc
     7141 gatgggtaaa gaagtggtgt atttaatctg ttccatcgcg aagctggaag tgacgtccga
     7201 cagccccggc acgctgttta ccagacgctt ataaaactcg tcgtagcgtt tcatgtcggc
     7261 aacctggacg cgcatcagat aatcgtattc accagccatg cgccagaccc ccagcacttc
     7321 tggcatttcg gtaaccaccg tgacaaagcg gcaataccat tcgctgctgt gatgttgcgt
     7381 ttttatcagc acaaaggcgg tcaggccgag gcctattttt tccggatcca gcagggcgac
     7441 tttgccgata aggataccgt cgtcctccag ccgtttcagg cgcttccagc agggggtggt
     7501 tgtcagatta acggcttcag ccagtgcctg caaagagagg gtgcaatcct gctgcagtaa
     7561 ggccagcagc ttacggtcaa ttttatctaa catagccctt ccacggagaa tttttttctc
     7621 ttttaattct attttaaagg tgaaatcgcg acaatttatt ctgtgcggtt taaacaccac
     7681 ggcacaaaat gacaaattca acgcaaaaaa aaaccgggca attgcccggt gctggaaggg
     7741 atctcgttat tcgggggaat aaggtagatg tggatagtcc agccagtgcg tcaaatagtg
     7801 agagacagcc tgatttcggc aatgtccaat caccggtaaa tgcgggagct ccgcgcgcag
     7861 ttgcggcatc gcattgccca taataaaccc gctaccgacg ctgcctaaca tttcgcgatc
     7921 gttcatcgca tcaccaaagg ccatgcaatc gcgcaacgat aaacctaaat gttgggtccg
     7981 caccgtcaat gcagcgcctt tattgcagcc caccggcagc acttcgaggc aatccgtggc
     8041 ggaaaaacac aaatgtgcac gctcgcctaa tgcttcgtat agctggatct gcaagcgtgt
     8101 aagatcgtcg tgatcgccac agaagcagat cttggtgacg ctgccgagtg gcattttttt
     8161 gacatcgatt atctgataac gaaaaccgct atagacaaat acctgcaaca acgcagggct
     8221 ctctttcccg gtaaaccaac cgtcgtcatt gaagatatgc atgctggctc gggtatccca
     8281 ttgctgatac agcaccagct ccgcgacatc cgcaggtaaa tcatcacgat gtaaaagttc
     8341 accttccaga gaatgcacgc gcgttccgtt gccggtaatc aaatatgcat ccagcgatag
     8401 cgccccgaga atatgctgca tctccagcgc atgacgcccc gtggcaaaag tgagggtaat
     8461 gtcgcgttca cgcagtcgcg ccaaagtaga gagggttttc tcacctaaat gatggtcagg
     8521 catcaataaa gtgccatcca tatcaaatgc taccagacga gccatttctt tctccactct
     8581 gtgacacggt taatggtgat tgagtatcac ctgatatata cggaagtaat agtgaatagt
     8641 taaataatat tgttccgggt ttatatgcga ttgctcaacc gtcttaacca gtatcaacgt
     8701 ctgtggcaac cttcagccgc aaaccgtcac cgtcagcgaa ctggccgaac gctgtttttg
     8761 cagcgaacgc catgttcgta cgctgttgcg tcaggcacag gaggcgggat ggctggagtg
     8821 gcaggcgcag tcaggacgcg gaaagcgcgg acaattacgc tttctggtca cgccggaatc
     8881 gctacgcaat gcgatgatgg aacaggcact ggaaaccgga aagcagcaag atgtgctgga
     8941 gctggcgcaa ctggccccag gtgagctgcg cactctgtta cagccattta tgggcggaca
     9001 atggcaaaac gatacaccca cgttgcgtat tccctactat cgcccgctcg aaccgctaca
     9061 accaggcttt ttgcccggcc gtgccgagca gcatctcgcc gggcagatat tttccggcct
     9121 gacccgcttc gataataata ctcagcgccc gattggcgat ttagcgcatc actgggaaac
     9181 ctctactgac aggttacgct gggactttta tcttcgttca accctacact ggcataacgg
     9241 cgatgcagta aaagcctcac acttacacca gcgattattg atgctgttac aactgccagc
     9301 actggatcaa ttatttatta gcgtgaagcg tattgaagtc acccatccgc agtgtctgac
     9361 cttcttttta catcgccccg attactggct tgcgcaccgg ctggcgagct attgcagcca
     9421 tctggcgcat ccgcaattcc cactgatcgg cactggtcct tttcgcttaa cacaattcac
     9481 agcggagctg gtgcgcctgg agagccatga ttattaccat ttgcgtcatc cgctgcttaa
     9541 agcggttgag tactggataa ctccgccgct tttcgaaaaa gatttgggaa ccagttgtcg
     9601 gcatcccgtg caaatcacca tcggcaaacc ggaggagctg caacgggtca gccaagtcag
     9661 tagtggcatc agtttaggtt tttgctattt aacgttgcgc aaaagtcccc gactctccct
     9721 ctggcaggcg cgaaaagtga tctccattat tcatcaatcc ggtttattac aaacgttaga
     9781 agtcggagaa aacctgatca ccgccagtca tgcattactg ccaggctgga ctattccgca
     9841 ctggcaagta ccggatgaag tcaaactacc gaaaaccttg acgctggttt atcacctacc
     9901 gatagaactt cataccatgg cagaacgcct acaggcgaca ctggcagcgg aaggctgtga
     9961 actcacaatt atttttcata acgcaaaaaa ctgggacgac acgaccctac tggcacacgc
    10021 agacctcatg atgggcgaca gattaattgg cgaagcaccg gaatatactc tggagcaatg
    10081 gctacgttgc gatccactat ggccacatgt tttcgacgct ccagcatatg cacatctgca
    10141 atcgacgctg gacgcggtgc aagtaatgcc tgatgaggaa aaccgattta atgccctgaa
    10201 agcggttttt agccagttaa tggcagacgc gacgctgacg ccgctgttca actatcacta
    10261 tcgcattagt gcccctcccg gcgtgaacgg tgtgcgactg acaccgcgcg gctggtttga
    10321 atttaccgaa gcctggcttc ccgcgccgtc gcaatgaata gctggtccgg gttaatcgca
    10381 ggcgttacca taacgctttt attgttcaag caggattatc tatgaaacgt gctgtcgttg
    10441 tgttcagtgg aggccaggat tccaccacct gtctggtgca ggcattacaa caatatgatg
    10501 aagtccattg cgtgacgttc gattacggtc agcgacatcg cgcagaaatc gacgtggcac
    10561 gcgaactggc gctgaaactg ggagcacgcg cgcataaggt tcttgatgta acgctgctca
    10621 acgagctggc ggtcagcagc ctgacacgtg acagcattcc ggtgcctgat tatgaacctg
    10681 aagccgatgg catcccgaat acgtttgtcc cagggcgtaa tattttgttc ctgacgctgg
    10741 cggcaatata tgcgtatcag gtaaaagcag aagccgtaat taccggcgtc tgtgaaacgg
    10801 atttctccgg ctacccggat tgccgcgatg agtttgtgaa agcactaaac catgccgtca
    10861 gtttgggcat ggcgaaagat attcgttttg aaacgccgct gatgtggatt gataaagcgg
    10921 aaacctgggc gctggcagat tattacggca aactggattt agtccgtaac gaaacgttga
    10981 cctgctataa cggcattaaa ggcgacggtt gcggtcattg tgcggcatgt aatttacgcg
    11041 ccaacggttt gaatcattat ctggccgata aaccgacggt gatggcagcg atgaagcaga
    11101 aaaccgggtt gaggtaatta ttccgggtgt cgccggatgc ggcttgagca tccggcacca
    11161 caaaacgttt acttaaccat ctgctccagc ttttcgcgca attccccttc cagagctaat
    11221 gctttctgcg ttttaagatc aatacaaaca aacgtaataa gcgcatccgc taccacctgc
    11281 ccttccggct ccagtgtaat gacctggttt aagatgccgc ttttaccgtt taattgctgc
    11341 aactgactgg taatagttaa caggtcgctt aataccgctg gacgacgata gttaatattg
    11401 atattgacca cgacgaaggc gatgttatgg gccgtcatcc actgaaaact gtcgctattt
    11461 tccaccccat cccagcgggc ttcttcgaga aattcaaggt agcgggcgtt gttgacgtgc
    11521 tggtaaacgt cgagatgata tccacgaact ttgatttgtg tttgcatagc gcaataacct
    11581 tacgttgttg ttataagtac agaggtcata actggtatga cctcttcagt ctggcaaaaa
    11641 attgccacta tgcaaattaa ttacagggtt aataccgcca gattacgttc caccagcgaa
    11701 ttgcccatcc ccggcacctg ctttagatcc tccaccgttt taaacggacc gtactcttcg
    11761 cgataactga caatcgcctg cgctttcttc aggccaacgc cattcatcgc gcgggctagc
    11821 tcttccgcgc tggcattatt aatgctgacc cgggtgcctt cttcgtcact ggctttcgcc
    11881 ggtactgctg ctttactttg tgctgcagga gcttccgctt tggtttctac cgccgtcggt
    11941 ttcgccacag aagctgccgc cagcgcgcta tgagacattc cggcacaggc cagggacagt
    12001 gtaatgagca gtgctttaat tccgtgtttc atactgtttt ctccttgttt gttaacagtg
    12061 gaatcacctt aacgggagtg aaaacagtaa acaaatggca atgttcagaa atggaaaagg
    12121 ccgcgaaagc gaccttttct tgttacagct gcaacgcatt tttttgcgag gcggcttcag
    12181 gattattgct gttccagcgc atcgccaatt ttgattttcg cctctttacg caggttactc
    12241 atcagagctt caaagacgat ttgtgcgttg ttctgggtga taccctgcac catcgctttt
    12301 ttctgatctt ccggcattga accttgtttc acttcatcca gcgccagcag aaccacatta
    12361 ccttgcatat cggtcgccat accgtagctc ggtttgtctt tcgctggcag tggcagtgca
    12421 aacgccgcct ggctaatcgg gtcacgaccg gaacggctta aggttttcgg ctcgccaaat
    12481 ttcagaccgg cagcctgcat agcttccgca cctttgccgg ctttcaaatc aaccagcagt
    12541 ttctcagcat ccactttcgc ctgttgttca gctttgttgt gctgaaccag tgccttaact
    12601 tgttcctgaa catctgccaa cggtttcacc gcttccggtt tgtgctcgct gacgcgcagc
    12661 acgaatgcgc ggtcgccgtc tacggtgatg atgtcagagt tgatgcccga cgcgccgttt
    12721 tcacctacca gaccgccgtt aaagatagcg tcggcaaccg gcttgaagtt caactcttcc
    12781 ggcaggttat ctttgctgaa ccaacccgtc tgagtggctt taacgccggc agcttgctct
    12841 gcaccggcca gagactcggt gtcgttgctt gccacatcgc tcactttctg ctgcagcgca
    12901 tagtacgcat cgagggcttt ttcgtgtttc actttcgccg caatgtcgtc acgtacttcg
    12961 tctaacgatt tcactttcgc tggctgaatg tcgtccagac gtacaatcag gaaaccgacc
    13021 gaagatttga tgacaccaga cagttggcct ttttctttca gaccagcatt tttcagttcg
    13081 tcggggatag tggcatcttc taaccaaccc atatcgccgc cgttacgggc agagataata
    13141 tcggcagatt tttctttggc taatgcagca aaatcaccgc ctttattcag ctcatcaagt
    13201 accgctttcg cttcatcttc agttttggtc tggatgatgc tgtagcgggt acgctgcggc
    13261 tgggtgaatt gatcctgatg ctgatcgtag tagctctgga tatccgcatc gctaaccggt
    13321 tgctgcatcg ttgcggcaac cagcttgatg taactcacgc ggaattgttc cggcgtcatg
    13381 aaattgtttt tgttttgttc gtagtagctg gcaatctcct gttcggtcac aggctgcttc
    13441 gccgccagcg cgttaacatc gatagtcgcc tcacgcacca cgcgttgttg cgcgaccagt
    13501 gccgccagct cgtcggtttc acctttcagc ataaaatcgg taccggcaac gccgttaatc
    13561 agctgttggg tagtgagctg gttacgcagc gcctgggcgt actgatcggc ggtcatcccc
    13621 atctggttga ggataccgtt atagcggctg ttatcaaatt tgccgtcaac ctggaaggct
    13681 ggggtcgcga aaatcgcctg tttaacctgc tcatcactga tacccagttt cagctcacga
    13741 gcgtactgat ccaacagcgc ctcgtcgatc agacgattca gcacctgttg acgcagggtt
    13801 ttcatatagc cttcgttcgc tgccagctcg gagtattgat cgcccagctg ttgctgcata
    13861 cgattacgct cgctgttaaa ggcgttctcg aactgcccac ggctgatttc ctggtcattc
    13921 acttttgcgg cgtagttatt gcctccgcca atcaggtaac cactcacgcc ggtcaatatg
    13981 aacgacacga taatgatacc gaaaataatc ttgagcacga gactgtttgc agccgtgcgt
    14041 aagctgtcca tcatggtgta acaacactcc gctgtagatg actgtgtacc tcacgcaacg
    14101 tatcgatgcg cgcaaaggcc tattgtgaca agaaacgggg gcaattgtca gcccacaaga
    14161 cacagaaagt caccgaatac aaataaaaaa ggcacatcag tagatgcgcc cttgaacttc
    14221 gtcacatccc cactggggac aacgcttagt ttaccgcgtc tttcagtgct ttacctgcac
    14281 ggaagctcgg tactttagcg gcagcgatgg tgatctcttt accggtctgc gggttgcggc
    14341 cagtacgggc agcacgctct ttaacggcaa aagtaccaaa acctaccagt gctacatcat
    14401 ccccttcttt cagagattca gttacggaag caataatagc atctaacgca cggccagccg
    14461 cagctttaga gatatcagcc cctgcagcaa tcttgtcgat caattgagat ttattcactc
    14521 ttctcttcct ctttataatt tatatcgcac ttgaatcctt caaggtacga acgcgcagca
    14581 gttatatcag gccagccatc cccttacaac accccttaat aagggcaagc ccgatccgct
    14641 atctaactta gcgagacaaa aaaaggctgg caagcccgaa ttagcctgcc agccctgttt
    14701 ttattagtgc attttgcgcg aggtcactat tttgcagtca caacctgcat gccagacggt
    14761 tcattttgca gcgccagagt cagaacttcc tcaatgcgct tcacaggatg aatgtccaga
    14821 tcggcaatta cgttgtcagg aatctcttcc agatcgcgtt tattttcgaa cggaattagc
    14881 actgttttaa tcccgccgcg atgcgctgcc aggagttttt ctttcaaacc accgatcggc
    14941 agtacctgac cacgcagagt gatctcaccg gtcattgcca catcggcacg aaccgggtta
    15001 ccggtcaggc aagaaaccag cgcggtgcac atagcaatac cggcactcgg accatctttc
    15061 ggcgtcgcac cttccggtac gtggacgtgg atgtcacgtt tttcgtaaaa atcagggttg
    15121 atccccagtt tttccgcacg cgcacgaacc accgttaacg ccgcctgaat agactcctgc
    15181 atcacttcgc cgagcgaacc ggtataggtc agtttgcctt tacccggaac acatgcggtt
    15241 tcaatggtca gcaagtcacc gcccacttcc gtccacgcca gaccgattac ctgaccgaca
    15301 cggttttcgt tatcagcgcg accatagtcg aaacgctgaa caccgaggta gtcatgcagg
    15361 ttatcgccgt taatttcgat atgttttaat gacttatcga gcagtaactg cttaaccgct
    15421 ttgcggcaca gtttggagat ttcacgctcc agaccacgca cgcccgcctc acgggtgtag
    15481 taacgaataa tgccgataat ggcgctatcg tcgacggtca gctcaccttt tttcagtgca
    15541 ttacgttcaa tctgcttcgg cagcaggtga cgtttggcga tgttcagttt ttcatcttcg
    15601 gtataaccgg agaggcgaat cacttccata cgatccagca gcggtgccgg aatgttcatg
    15661 gagttcgacg tcgcgacaaa catcacgtcg ctgagatcgt aatccacttc caggtagtgg
    15721 tcgctgaacg ctacgttctg ctctggatcc agcacttcaa gcagtgcaga ggccggatcg
    15781 cctcgcatgt cagaagacat tttgtcgatc tcatcgagca ggaacagcgg atttttcacg
    15841 cccactttcg ccattttctg gatcagttta cccggcatag aaccgatgta agtacggcgg
    15901 tgaccacgga tttccgcttc atcacgcacg ccgcccagcg ccatacggac atatttacgc
    15961 ccggtggctt tggcaatgga ctgaccaaga gaggttttac ctacccccgg cggccctacc
    16021 aggcagagga tcggtccctt gattttgttg acacggcttt gaaccgcaag gtactcaagg
    16081 atgcgatctt tcacgcgctc cagaccataa tggtcggtat caaggatttc ctgcgcctga
    16141 cgcaggtctt ttttgacctt gctgcgcgca ttccacggta cctgtaccat ccagtcgata
    16201 taaccacgca ctacggtcgc ttctgccgac atcggagaca tcattttcag cttctgcaac
    16261 tctgcttccg ctttctcttt tgcctctttc ggcatcttcg ccgcgtcgat tttgcgcttc
    16321 agggcttcgt tttcgtccgg cacgtcgtcc atttcaccga gttctttctg aatagctttc
    16381 atttgctcgt tcagatagta ctcacgctgg gatttctcca tctgcttttt aacgcggttg
    16441 cgaatgcgtt tctcaacctg cagcagatcg atttccgatt ccatcattgc catcagatat
    16501 tccagacgtt cgttaacgtc ggacatctcc agaacggact gtttgtcagc cagtttcagc
    16561 ggcatatgtg cagcaatggt atccgccaga cgcgccggat cgtcgatgct attcagcgac
    16621 gtcagcactt ctggtgggat ttttttgttc agcttgatgt agccttcgaa ctggctgatt
    16681 gcagtacgca ccagcacttc ctgttcccgc tcatcaatgg tcggcgactc cagatactcc
    16741 gccttcgcag aaaagtgttc gccattgtca gagagcgcag aaatacgcgc gcgctgtaac
    16801 ccctcgacca gcactttgac ggtgccgtca ggcagtttca gcatctgcaa tatagaggcc
    16861 acggtcccga cggtgaaaag atcgtttaca cccggctcat ccgttgaagc ttctttctgc
    16921 gcgaccagca taattttttt atcatggtcc atcgccgctt ccagacaacg gatagatttt
    16981 tcccgcccga caaataaggg gatgaccatg tgcggataaa ccaccacatc gcgcagcggc
    17041 aatacgggga tttcaatgcg ttcagaacgc tcaggattca tagagctctc tcttagttta
    17101 atttccgcca ggtaatcaga tgacacgact gtgcttcacg ccatctatta acatgtacgt
    17161 cagtatatgg ggatgtttcc cccacattca acgccgagaa tagaggaaaa attaaagggg
    17221 agataaaatc cccccttttt ggttaactaa ttgtatggga atggttaatt attcaccaga
    17281 tgcctgttgc gcttccggct taccataaat cagcaacggt ttgctttgac catcaattac
    17341 cgactcgtcg ataaccactt tttcgacgtc ttccatggac ggcagatcgt acatggtatc
    17401 gagcagtgcg gcttctacga tggaacgcag gccacgggca ccggttttac gcgccatcgc
    17461 tttcttagcg atagcatcca gcgcctcgtc acggaattcc agatccacgc cttccagatt
    17521 aaacagcgcc tgatactgct tggtcagggc gtttttcggc tctttgagga tctgaatcag
    17581 agcttcttcg ctcagttcat tcaacgttgc tacaaccggc agacgaccaa tgaactcagg
    17641 gataagacca aacttgatca gatcttccgg ttcaacctgc gccagcagct cgccttcgct
    17701 tgctttgtcg gacttcgctt ttaccgtcgc gccaaaacca atgccggagc cggtttctac
    17761 acggtgggaa atcactttat ccagaccggc aaacgcaccg ccacaaataa acaggatctt
    17821 agaggtatca acctgcaaga attcctgctg cggatgttta cgcccacctt gcggtggaac
    17881 agcagctacc gtaccttcga tcagtttcaa cagtgcctgc tgtactcctt caccggaaac
    17941 gtctcgggta atggacgggt tgtctgactt acgagaaatc ttgtcgattt catcgatgta
    18001 gacaatcccg cgctgcgctt tctgtacgtc gtaatcgcac ttttgcaaca gcttctgaat
    18061 gatgttttca acgtcttcgc ccacataacc ggcttcggtc agcgtggttg cgtcggccat
    18121 ggtgaacggg acgtccagca ggcgcgccag cgtttcagcc agcagcgttt taccggaacc
    18181 ggtcggaccg atcagcagaa tgttactttt gcccaactcg acaccattgc tggtatcgcc
    18241 gttgcgcaga cgtttgtaat ggttgtatac cgcgaccgcc agcacttttt tcgcctgttc
    18301 ctggccgata acgtaatcgt ccaggtggtt gcgaatttca tgcggcgtcg gtagcgcact
    18361 gcgttcacga tgcggtgcaa cttctttaat ctcttcgcga atgatgtcgt tacataaatc
    18421 aacacattcg tcgcagatat acacggatgg accggcaatc agcttgcgca cttcatgctg
    18481 gcttttgccg caaaaagagc aatacagcaa tttgcctgag ccatctttgc gtttatctgt
    18541 catgggtcaa aacctcttct ttgttctttg tgccgcacac gacgacgcaa atgccattct
    18601 caggcgcaag ccgcttacag cgttgtgccg ccctagataa gtatagcggc acagttgcgc
    18661 ctctggcatc aattacgatg ggtcagaatc gaatcgacca gaccgtattc caccgcttca
    18721 ggggcggaaa ggaagcgatc gcgctcggta tcacgttcaa tctgttctaa tgattgaccc
    18781 gtatgaagcg ccataagttc attcatgcgc cctttaactt tcagaatttc acgggcatga
    18841 atttcgatat cggtcgcctg gccctggtag ccgcccaacg gttggtgaat catcacgcgc
    18901 gaattcggca ggcaaaaacg tttacctttt gcccctgcgg tcaacaagaa agcgcccatc
    18961 gaggccgcct ggcccataca gatggtgctg acatcaggct tgataaactg catggtgtca
    19021 tagatagaca tcccggcagt gatcaccccg cctggggagt taatgtacag atagatatct
    19081 ttttctgggt tttccgcttc caggaacagc atctgcgcca caatcaggtt agccatgtgg
    19141 tcttcaacct ggccagtcag aaaaatgacg cgttccttaa gtagacgaga atagatatca
    19201 aaagagcgct caccgcgtga ggtctgttca atgaccatcg gcaccagcgc catatggggt
    19261 gcaaagttat ctcgttcgcc gctgtatgac atttccgtct cctggataaa attgaaaaaa
    19321 acctgctgta ccgattgtaa ccttatggac ggattatcag ctagtccctg tgttatgggt
    19381 acggcatcac catatttcaa gcataacaat cttttgttgt tacgctaaca ccgaaagagg
    19441 cttttcgcac ggctccatgc aaaattcatg acaaaaaaaa gcccaccacc tggcggtgac
    19501 gggcctttgt gcgaatttag cgcgttatgc tgcgtaaatt acgcctgctg gttcatcagc
    19561 tcgttgaaag tggtttcttt ttcagtcact ttcgctttcg ccagtacagc ttcaacagcc
    19621 tgttcttcca gagcaacatt gcgcatgttg tccatcagtt ctttgttttt gctgtagaac
    19681 tcgataactt ctttcggatc ttcgtacgca gaagccatct cttcgatcag gcctttcacg
    19741 cgctcttcgt cagctttcag ctcgttggtg cggataactt cgcccagcag caggccaaca
    19801 actacgcggc gtttagcctg ttcttcgaac agttcgcgcg gcagttccag agcttgtttt
    19861 tcgttgccac cgaaacgctg tgcagcctgg cgacgcagaa cgtcgatttc gctgtcgatc
    19921 agcgcagccg gtacgtcgat gtcgttagct tttaccagac cttcgatcgc ctgagactta
    19981 acgcggttac ggatggcgct cttcagctcg cgctccatgt ttttacgcac ttcagcgcgc
    20041 agaccttcta cggaaccatc ttcaacgccg aaacgtttga tgaattctgc agtcagttcc
    20101 ggcagttcac gctcttcaac tttcttcagg ttgatagcga atttcgctgc tttacctttc
    20161 aggttttctg cgtggtattc ttccgggaag gtcacgtcga tggtgaactc ttcgccagct
    20221 ttgtggcctt tgataccgtc ttcaaagccc gggatcatac gaccctggcc catcgccagt
    20281 acgaaatcag acgctttacc gccttcgaac tcttcgccgt ctacagaacc ggtgaagtcg
    20341 atggttacgc ggtcttctgc ttcaacagcg ccgtcttttt ctttccaggt cgcctgctgt
    20401 ttacgcagag tatccagcat gccgtcaacg tcagcgtcgg tcacttcaac gatcggtttt
    20461 tcaacttcga tcgcttccag accctgcagt tcaacttccg gataaacttc aaactctaca
    20521 gagtaagtga agtcttcacc cagcttgtat tcgcccggaa cataagtcgg tgcgccagcc
    20581 ggattgattt tttctttaat gatggcgtca atgaagttac ggctcatcag gtcacccaga
    20641 acgtcctggc gtacagacgc gccataacgc tgagcaacga tattcattgg cactttgcct
    20701 ttgcggaagc cgtcaatacg tacttttttc gcaacgttga ccagctcgct tttaacagcg
    20761 gtctcgatgc tgtcagcagc gatagtaatc gttacacggc ggccaaggcc ttgagtggtt
    20821 tcaactgaaa cttgcatctt gttacctcaa aaaatcacag tgctcggtca actcttaccc
    20881 cgcaagcaca ggttgttggc ttcgcggaac cgggatgttc ttataatcaa tcacattccc
    20941 tgttaccaga atcatcccga aagcattcaa ataggacgcg gcattatagc ggcatcactt
    21001 ttatgagtcg agaacggtta ttgcgcgctg tcgcgtattt tttcacaatt cccgggcaat
    21061 tttcgtctga atgctgggca aaaagcgcaa aatccagaca aaacaaaacg gcccgcaggc
    21121 cgtttttcat aaaatcttta gcaacatact ggaaaagcgc cgacagttgc aaatgcgttt
    21181 ttacgcgatg cttcctgctc cacgacaggg aggagaggca aagacggtgt cctgcaaccc
    21241 ttcccactcc ttaatagtgt aagtatgcag agccagcgca tgaacggtag tagagagttc
    21301 ctccgctaaa gtactgtaaa tcattcgatg acgattcaga aaacgttcac ccgtaaaacg
    21361 atcgctgacc agcacaactt taaaatggct ttcagagccg gctgggacat tgtgacgata
    21421 gctttcatcc actacttcga ggaatacggg ttggaacgcc gcccttaatt tttcttctat
    21481 ccgctcacgt atcatcatga aatatctcct ccgacaacgc tgagatgtca tccatccctt
    21541 taaatactag ccgcttttac cgtttccatt gcagcttaac aacaaatatt taggtttcgt
    21601 cctggttttt ccgtcaagcg tatgaagttt actgatttaa agattgaaac acaggcctcg
    21661 catcagcgtt tcttttacca ggggtgatta tttcgtcaac atgatgaatt tacatgcctt
    21721 acccacttcc cctcgccgtt ggcaatgtta tgatggcggg aatttttacc aaacacgctt
    21781 aagagtctct gagagcccga acatgttcaa aaaaatcctc ttcccgttag ttgctctgtt
    21841 tatgcttgca ggatgcgcaa aaccgccaac aactattgaa gtttccccga cgattacact
    21901 gccacagcag gatccaagcc tgatgggcgt caccgtaagc attaatggtg ccgatcagcg
    21961 taccgatcag gcgctggcaa aagtcacccg cgataatcaa atcgttaccc tgaccgcctc
    22021 ccgcgctctg cgtttcctgc tgcaagaagt gctggaaaaa cagatgaccg cgcgtggtta
    22081 catggttggt ccgaatggcc cggttaatct gcaaatcatc gttagccaac tgtatgctga
    22141 cgtgtcccag ggcaacgtgc gctacaacat cgctaccaaa gcggatatcg ccatcatcgc
    22201 taccgcgcag aatggcaaca aaatgaccaa aaactatcgt gccagctaca acgttgaagg
    22261 tgcgttccag gcctccaaca aaaatatcgc cgatgcggtt aacagtgtgc tgaccgatac
    22321 catcgctgat atgtctcagg acaccagcat tcacgaattc atcaagcaga acgcgcgtta
    22381 atttctgccc tctggcccgg tgcaagccgg gcctgtagac gcccatgtcc agtcaatatt
    22441 tacgtatttt tcaacagccg cgttcagcca tattgctgat cctgggtttt gcttccgggc
    22501 taccgctcgc cctgacatcc ggcaccttac aggcctggat gacggtcgaa aatatcgatc
    22561 tcaaaaccat tggtttcttc tctctggtag gccaggctta cgtttttaaa ttcctctggt
    22621 caccgctgat ggaccgctac acgcccccat tttttgggcg gcggcgcggt tggctgctcg
    22681 ccacgcaaat cctgttatta gtcgccattg cggcgatggg ttttctcgaa ccaggcaccc
    22741 aactccgctg gatggcggcg ctggcggtgg tgatcgcttt ttgctctgcc tcccaggata
    22801 ttgtcttcga tgcgtggaaa accgatgtgc tcccggcaga agaacgtggt gcgggcgcgg
    22861 caatcagcgt gctgggttac cgtttaggga tgctggtttc cggcggccta gccctgtggc
    22921 tggcagataa atggttgggc tggcagggca tgtactggtt gatggcggcg ctgttgatcc
    22981 cctgtattat cgcgacgttg cttgcaccag aaccaaccga caccattcct gtgcccaaaa
    23041 cgctggaaca agcggttgtt gcacctctgc gagatttctt tggtcgcaat aatgcctggc
    23101 ttattttgct tcttatcgtg ctgtataagc tgggcgacgc attcgccatg agcctgacaa
    23161 ccacgttttt gattcgcggc gtcgggtttg atgcgggtga agtaggcgtg gtaaacaaaa
    23221 cgcttggctt actggcgacc attgttggcg cattgtacgg tgggattttg atgcagcgcc
    23281 tgtcgctgtt ccgggcgctg ctgattttcg gcattttaca aggtgcgtct aacgctggtt
    23341 actggctgct gtcgattact gataagcatc tctacagcat gggcgcagcc gtctttttcg
    23401 aaaacctctg tggcgggatg ggcacatcag cctttgtcgc gctgttaatg acgctgtgta
    23461 ataagtcatt ttccgctact caatttgccc tgctctcagc gctttccgct gtagggcgag
    23521 tttatgtcgg ccccgtggcg ggttggtttg ttgaagcaca cggctggtcg acattctatc
    23581 tattctccgt cgccgctgcc gtaccggggc ttattttgct gctggtttgc cgccagacgc
    23641 ttgaatatac acgagtaaat gacaacttta tctcccgtac cgaatatccg gcaggttatg
    23701 cctttgccat gtggacactg gcggcgggcg tcagcctgtt ggccgtgtgg ttacttctgt
    23761 tgacgatgga cgcgctggat ttgacgcact tctctttcct gcctgctctg ctggaagtcg
    23821 gggttttagt cgccctttct ggcgtcgtgc ttggtggttt gctggattat ctggcgctac
    23881 gaaaaacgca tctgacgtaa tctgtaaata ttatttagct gtagtaatca ctcgccgtaa
    23941 ttattacggc taaataaaca tcagcatcgc ttattaatat ttgttctttt gtgcggctta
    24001 gcgtttggaa attattgacg ccatttataa attctatttt tcttacacga ttcagctaat
    24061 gagtctttat tttctcacca cccagttgtc actttaatga taattatttg ttaaataatt
    24121 gttttatttc gcattggtta taccaattgc ccaccaagac tccttctgca ctcccctttt
    24181 gtta
//
LOCUS       DAZSIO010000048        23881 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZSIO010000048 DAZSIO010000000
VERSION     DAZSIO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.048"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(2..298)
                     /locus_tag="ACHX5T_001761"
     CDS             complement(2..298)
                     /locus_tag="ACHX5T_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFR8466995.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            513..1241
                     /gene="tonB"
                     /locus_tag="ACHX5T_001762"
     CDS             513..1241
                     /gene="tonB"
                     /locus_tag="ACHX5T_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR8466996.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="ACHX5T_001763"
     CDS             complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="ACHX5T_001763"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR8466997.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1784..2323)
                     /locus_tag="ACHX5T_001764"
     CDS             complement(1784..2323)
                     /locus_tag="ACHX5T_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR8466998.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2353..3096)
                     /locus_tag="ACHX5T_001765"
     CDS             complement(2353..3096)
                     /locus_tag="ACHX5T_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR8466999.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(3124..3213)
                     /locus_tag="ACHX5T_001766"
                     /pseudo
     CDS             complement(3124..3213)
                     /locus_tag="ACHX5T_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            3453..4091
                     /gene="ompW"
                     /locus_tag="ACHX5T_001767"
     CDS             3453..4091
                     /gene="ompW"
                     /locus_tag="ACHX5T_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR8467000.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(4151..4657)
                     /locus_tag="ACHX5T_001768"
     CDS             complement(4151..4657)
                     /locus_tag="ACHX5T_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFR8467001.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPFIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(4703..5203)
                     /locus_tag="ACHX5T_001769"
     CDS             complement(4703..5203)
                     /locus_tag="ACHX5T_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFR8467002.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(5289..5468)
                     /locus_tag="ACHX5T_001770"
     CDS             complement(5289..5468)
                     /locus_tag="ACHX5T_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR8467003.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(5848..6654)
                     /gene="trpA"
                     /locus_tag="ACHX5T_001771"
     CDS             complement(5848..6654)
                     /gene="trpA"
                     /locus_tag="ACHX5T_001771"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR8467004.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(6654..7847)
                     /gene="trpB"
                     /locus_tag="ACHX5T_001772"
     CDS             complement(6654..7847)
                     /gene="trpB"
                     /locus_tag="ACHX5T_001772"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR8467005.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(7859..9220)
                     /gene="trpCF"
                     /locus_tag="ACHX5T_001773"
     CDS             complement(7859..9220)
                     /gene="trpCF"
                     /locus_tag="ACHX5T_001773"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR8467006.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(9221..10816)
                     /gene="trpD"
                     /locus_tag="ACHX5T_001774"
     CDS             complement(9221..10816)
                     /gene="trpD"
                     /locus_tag="ACHX5T_001774"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR8467007.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(10816..12378)
                     /gene="trpE"
                     /locus_tag="ACHX5T_001775"
     CDS             complement(10816..12378)
                     /gene="trpE"
                     /locus_tag="ACHX5T_001775"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR8467008.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(12400..12493)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(12470..12514)
                     /gene="trpL"
                     /locus_tag="ACHX5T_001776"
     CDS             complement(12470..12514)
                     /gene="trpL"
                     /locus_tag="ACHX5T_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR8467009.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            12652..13533
                     /gene="rnm"
                     /locus_tag="ACHX5T_001777"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             12652..13533
                     /gene="rnm"
                     /locus_tag="ACHX5T_001777"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR8467010.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            13530..14150
                     /locus_tag="ACHX5T_001778"
     CDS             13530..14150
                     /locus_tag="ACHX5T_001778"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR8467011.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            14251..15123
                     /gene="rluB"
                     /locus_tag="ACHX5T_001779"
     CDS             14251..15123
                     /gene="rluB"
                     /locus_tag="ACHX5T_001779"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR8467012.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(15163..15753)
                     /gene="cobO"
                     /locus_tag="ACHX5T_001780"
     CDS             complement(15163..15753)
                     /gene="cobO"
                     /locus_tag="ACHX5T_001780"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFR8467013.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(15750..16508)
                     /locus_tag="ACHX5T_001781"
     CDS             complement(15750..16508)
                     /locus_tag="ACHX5T_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR8467014.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            16728..17777
                     /gene="sohB"
                     /locus_tag="ACHX5T_001782"
     CDS             16728..17777
                     /gene="sohB"
                     /locus_tag="ACHX5T_001782"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR8467015.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(17813..18064)
                     /locus_tag="ACHX5T_001783"
     CDS             complement(17813..18064)
                     /locus_tag="ACHX5T_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR8467016.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            18444..21041
                     /gene="topA"
                     /locus_tag="ACHX5T_001784"
     CDS             18444..21041
                     /gene="topA"
                     /locus_tag="ACHX5T_001784"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR8467017.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            21251..22225
                     /gene="cysB"
                     /locus_tag="ACHX5T_001785"
     CDS             21251..22225
                     /gene="cysB"
                     /locus_tag="ACHX5T_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR8467018.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            22544..22684
                     /locus_tag="ACHX5T_001786"
     CDS             22544..22684
                     /locus_tag="ACHX5T_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR8467019.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            22687..22854
                     /gene="yciX"
                     /locus_tag="ACHX5T_001787"
     CDS             22687..22854
                     /gene="yciX"
                     /locus_tag="ACHX5T_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR8467020.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            22968..23063
                     /gene="ymiC"
                     /locus_tag="ACHX5T_001788"
     CDS             22968..23063
                     /gene="ymiC"
                     /locus_tag="ACHX5T_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR8467021.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            23227..>23881
                     /locus_tag="ACHX5T_001789"
     CDS             23227..>23881
                     /locus_tag="ACHX5T_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFR8467022.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     "
BASE COUNT         6020 a         5885 c         6089 g         5887 t
ORIGIN      
        1 ctcagaacac tttcttaaat ggtttcactg aaacgtgttc atagactcct gccgctacgt
       61 acgggtcagc atcggcccag gcctgagctg cttccagcga ttcaaattca gcaataacgg
      121 ttgagccagt aaatcccgca gcccctggat cgttactgtc taccgctggc attggaccag
      181 ctgtcaacaa acgaccttca tcatgcagta actgtaaacg tgctaaatgt gccggacgaa
      241 cggaaaggcg tttttcgagg ctatcagctt tgtcttgagc gtaaataaca tacaacacgg
      301 gcacaactcc ttgttcggga aagttgtaaa gtacgttatt tgaaagggcg aagatctgca
      361 acggaaagat gatgtctttg ttaaggtcat gcataaagta agggtaatta cgccaaaaat
      421 gacattttca ctgatcctga tcgtcttgcc ttattgaata tgattgctat ttgcatttaa
      481 aatcgaggcc tagtttttct actgaaatga ttatgacttc aataaccctt gatttacctc
      541 gccgcttccc ctggccgacg ttactttcgg tctgcattca tggtgctgtt gtggcggggc
      601 tgctctatac ctcggtacat caggttattg aactacctgc gcctgcgcaa ccaatttctg
      661 tcacgatggt tgcgcctgct gatctcgaac cgccacaagc cgttcagccg ccaccggagc
      721 cggtggtaga gccagaaccg gaacctgagc cgatccccga accgccaaaa gaagcaccgg
      781 tggtcattga aaagccgaag ccgaaaccta agccaaaacc gaagccggtg aaaaaggtac
      841 aggagcagcc aaaacgcgat gtcaaacccg tagagtcgcg tccggcatca ccgtttgaaa
      901 atacggcacc cgcgcgcccc acatcaagta cggcaacggc tgcaaccagc aagccggtta
      961 ccagtgtggc ttcaggacca cgcgcattaa gccgtaatca gccgcagtat ccggcacgag
     1021 cacaggcatt gcgcattgaa gggcaggtta aagttaaatt tgacgtcacg ccggatggtc
     1081 gcgtggataa cgtacaaatc ctctcagcca agcctgcgaa catgtttgag cgtgaggtga
     1141 aaaatgcgat gcgcagatgg cgttatgagc cgggtaagcc aggcagtggg attgtggtga
     1201 atatcctgtt taaaattaac ggcaccaccg aaattcagta agcagaaagt caaaagcctc
     1261 cgaccggagg cttttgacta ttactcaaca ggtaaggcgc gaggttttcc ttcaggatca
     1321 accgctacat acttaaataa tgcttctgtc gctttatagc gttgcccgat tggttcagac
     1381 gctacttttt tcacccacac ttcaatatta atgctgaccg atgtcgtccc tttctggaca
     1441 cagcgtgcat agcagcacac cacatcgccg accgcaaccg gccgtaagaa agtcattcct
     1501 tcaacccgca cagtcactac gcgaccgtgg gcaatttctt ttgccagaat agcgccgcca
     1561 atatccattt gtgacattaa ccaaccacca aagatgtcac cattggcatt ggtatcggcg
     1621 ggcatggcta aagtacgtaa aacaagatcg ccctgaggga cgttatgtgt tgtagacatg
     1681 gtaaaaccga cttaaaagga aaatcacagg cgcgatgcta ctatgatttt cctgaggaga
     1741 acagaggaga tacagcgcca gccccgaagg actggcagtc tggttaggat ttatcttcct
     1801 gcggcatgtg gcggtagata tagataccgc ttaacaatgt gaagattaag gtaagggcgg
     1861 tcaggccaaa gactttaaag ttgacccaaa tattttgcgg cagccagaat gcgatgtaga
     1921 tgtttgccag accgcaaagg ataaagaaaa cagcccaggc cagatttagc ttcgaccata
     1981 ccggttgcgg cagcgtgagt tctttactca gcatccgctg aattagcggc tttttcatca
     2041 cccattggct gactaacagg gctcccgcaa acagagcata aatgactgta accttccatt
     2101 taataaactc atcattgtgg aagaacagcg tcaagccacc gaagacgacc accagaacaa
     2161 aagtgatcag ggccatcttc tcaaccttac gaaagcgaac ccagctatag ataagcacaa
     2221 tcgccgtggc gacgatcagc gctgcagtag ccgcatagat gtcataaatc ttgtaaaacg
     2281 cgaaaaagac aaccagcggt aaaaaatcaa gaaactgctt cattttacga ttccgtaatc
     2341 aaatgcttaa ggttattggc gaatcaacat atacaggcgg aacagataga tgagcaatat
     2401 ggcagaaatc aggttgctca aggtgttcgc cagtacggca ccaatttccg gggttaatgc
     2461 ggcaaaagaa gaggcaaaaa gcagcaacag tgtttttgcc agcaaccagc tcagtactgc
     2521 gggtgccacc agacgcatat tcgcccaagt cagccgcata ctgctacgca tcgaggcaaa
     2581 aatgcccatt ttgtcctgta ccagcatcac cggtgccagg gccagtaaaa tggccatgat
     2641 aattcccgga acgaccacca gcatgatgcc aatctgtact aaaagggtag tcagaaaaat
     2701 cagaataaat aacttgggca atatcggcgc actggcacca atagctcgca gcgcactgac
     2761 tctctgaccc gcagacacca gctggataat taatattacg cctccggcga gaatggcgtt
     2821 accgattaat tctgaaaaag tggacgccgc tgaagcctgc agcaaaattt gttgctgttc
     2881 cggtgacata ttctgaacca ggtcgaacaa cccactactg ccgctaacgg gcacgccgtc
     2941 attgagctgc gcaagctgtg catcactggg tgagaaaaca tgccctaaca ccactgtgat
     3001 aaacgcacat agcaacgata ccaacagaat ggtcataaat tgattacgga agaaatttcc
     3061 ggtgtcacgg tatacggact gcgccgtgat agacatgcac tctccttgag ttttatgcag
     3121 gtgttaatta gcgggcaatt gtaccctggt taagcctcgg gtggcagcat caggtcttgt
     3181 atggaaaagc atatctttgt aaagcggagg taattgctgg ccttcaggcg tgggcaaagc
     3241 gcggcaagtg gcgatcgagt taatatttgc gtagagaaaa tatttaaaaa ttgatttaaa
     3301 tcacattaac caggattctc aatgcaactt ctaaattaat ccagatcaat aaatggtgaa
     3361 ttatcatatg taatgtgatc tatgtaggat catttgttac tccaatgtag gtatattcgt
     3421 cacgttttta taaccataac gacggagcgg atatgaaaaa gttaacagtg gcggctttgg
     3481 cagtaacaac tcttctttct ggcagtgcct ttgcgcatga agcaggcgaa ttttttatgc
     3541 gtgcaggttc tgcaaccgta cgtccaacag aaggtgctgg tggtacgtta ggaagtctgg
     3601 gtggattcag cgtgaccaat aacactcaac tgggcttgac gtttacttat atggcgaccg
     3661 acaacattgg tgtggaatta ctggcagcga cgccgttccg ccataaaatc ggcacccggg
     3721 cgaccggcga tattgcaacc gttcaccatc tgccgccaac actgatggcg cagtggtatt
     3781 ttggtgatgc cagcagcaaa ttccgtcctt acgttggggc cggtattaac tacaccacct
     3841 tctttgataa tggatttaac gatcatggca aagaggcggg gctttccgat ctcagtctga
     3901 aagattcctg gggagctgcc gggcaggtgg gggttgatta tctgattaac cgtgactggt
     3961 tggttaacat gtcagtgtgg tacatggata tcgataccac cgccaattat aagctaggcg
     4021 gtgcacagca acacgatagc gtacgcctcg atccgtgggt gtttatgttc tcagcaggat
     4081 atcgttttta attccgcaca aaaacgaccc cgtaatatac ggggtcaata aggacatggt
     4141 ataaagggta ttatttcttc gcttctacgc catcagtttc agaacgaatt aaaaatttct
     4201 cagttgtttg cggaatatgc tgaatcagcc agtcggccat ttgcttttcc tcatttaaaa
     4261 tcgcttcgat gaacggaatt gagactgtat caccggcatt ttttgctgct gctaatagtg
     4321 aggtgtaaca ggtgatttca aattgctcga agacatatcc gccaatagag cctttaacta
     4381 tttcatcaga agggaatata ccaccgattg actgcccaag cgcagccatt ttactcatgg
     4441 aatctttaat gactgaacgt gaaatgtcat tacgatcaag aatagtttcc agttgaacaa
     4501 tctggttttt ggtttcacta agatgttgtt caatacgagc gcgtagttca ggataattat
     4561 ctatacggct ggccatggat tcaagcatag attcggcttg cttttccatt gcgtgggcgt
     4621 cacgtaacca gtcatgataa tgttcaatac gattcatgaa aatgtcctct ttataaaatg
     4681 actaaaagtt aaaattcata tttcaggctt tattttcggc tttcttattt acgttattaa
     4741 gggccagatc agtcagtttg atgtcggtgg ccttttcttc ttccagggtt tctttcagaa
     4801 gcttcgctgc tttacggtaa cctaattgtt cagccagcgt cgctaatgtc ccgtaactgg
     4861 caatctcata atgctcgact ttctgtgctg cggcaatcag tgcggcatca cgcacttcgt
     4921 ttttctcggt actttcgatg acctcattag cttcttcaat aagaccttcc attgccacac
     4981 atttcatgcg cttaattttc agatttgatt ctgattccac aacttgatca attcgttcaa
     5041 tctgtccatg agtttcctcg aggtgcgcat gaaaagcctg acttaatttt tcatttgatg
     5101 ttgctcttgc gagttttgcc agtgcccggg ttaattgttt ttctgcgctg taggtatctg
     5161 aaagcagttg aataaataca tcttcaatgg tcttcatatt catatttttc tccagtgaaa
     5221 tcactgcgag ccatacggcc cgcagatgca attatcttta tcagctgata ttaataatta
     5281 acggtgaatc aggatttgcc tgatttatta ccaccgcttt gttgaccgcc ttttttaccc
     5341 gcttcagatg cgcgttgcgg atcattttta aaattaccgc cgctatgctg accgccttta
     5401 cggcctgcgt cggatgcctt ctcacggtct tcggcgaaat ttcctgaact accacgatgt
     5461 tcggccatgt tatttctccc gttgcgttgc attgtttcat taatatgagt gttgtgtgtc
     5521 gacactcatt aaaattagtc actaatgaga attagtcaaa ttaagcgcaa cgagaagata
     5581 gagggaaata tattttgagg aacattctgg atatattaac aattacctga ggaatatgtg
     5641 acttagagaa aattattgat tttactggtg ttatgttgcg ggattaattt gtttgattat
     5701 attactgttg ggcggaaaaa tgacgtaagt tgacgttcga ccggggtaag cgaaacggta
     5761 aaaagataaa tattaaatga atttaggatt tttccggctt cattaaagaa agttaaaatg
     5821 ccgccagcgg aactggcggc tgtgggatta actgcgcgtc gccgctttca tcggttgtac
     5881 aaaaactttc agtgccgcca gcattttctc tggctcatta atatgttgct cgatgatttt
     5941 aacaatggcc gaaccagaaa tcgcgcccgc agctcctgca tcaatcgctg cttttacctg
     6001 atccggggcg gaaataccaa acccctgcaa tggaggtgca gcgttgtact ctttcagctt
     6061 cgcaaccaga tgattgaggg gtaacgcggc gcggttttct gcgccggtca cgcccgctcg
     6121 tgacagcaaa tatgtgtaac cacgaccgta agaggctatc tggcgcagca ggtcgtcatc
     6181 ggcatttggc gggcagatga agataggtgc gacattatgg cgcaacgcgg cctggcggaa
     6241 gggcgcggac tcttcaactg gcacatcggc aaccagcacc gaatcgacgc cgactttctc
     6301 gcactgggca taaaactcat caatgccttt gttaaacacc agattggcat acatcaacag
     6361 gccaatggga atggtcgggt gtttctggcg aatcagtgcc agcatttcaa aacattgtgc
     6421 cggagtcaca cctgccgcaa aggcgcgcag agtggcgttt tgaatggtcg ggccatccgc
     6481 cagtgggtcg gagaagggga tacctaactc cagcgcgtca gcaccggctt caattagcgt
     6541 atcgataatt ttcaatgact gctcaatgcc cggatcaccg agcgtgacga aaggaacgaa
     6601 tgcgccttct ttgcgctcct tcaactgggc aaacagagat tcgtagcgtt ccatcagatt
     6661 tcccctcgtg ctttcaaaat atcgtgaacg gtgaagatgt ctttatcgcc gcgaccggaa
     6721 aggttaacca ccagtagctg ctctttatcc gggttttcgc gcatcatttt cagcgcatgg
     6781 gccagggcgt gggaggattc cagcgccggg atgatccctt cgtgcaggca cagcgttttg
     6841 aaggcttcaa gggcttcatc atcggtaata gacacgtaat cagcgcgtcc agtgctgtta
     6901 agatacgcgt gttgtggacc gacagacggg aaatccagtc cggcggagat ggagtaagat
     6961 tcttcaatct gcccgtcttc ggtttgcatc atcggcgctt tcataccgaa ataaatgccc
     7021 acgcgaccat gttttagcgg tgcgccgtgc tcgccagttt cgataccgtg accaccaggc
     7081 tccacaccaa tcaggccgac gttggtttca ttgatgaaat cagcaaacat gccgatggca
     7141 ttcgaaccgc cgccaacaca ggcgataacg gcatccggca ggcgaccttc tctttccaga
     7201 atctgcgctt tggtttcttc gccaatcatc cgctgaaact cacggacaat ggtcggataa
     7261 ggatgcgggc cagccgcggt gcccagcata tagtgcgcgg tttcataact gccggaccag
     7321 tcgcgtagcg cctcattaca ggcatctttc agcgtggcgg aaccgctatg taccgggatc
     7381 acttccgcac ccattaagcg catacgaaaa acgttaggcg actggcgttc aacgtctttg
     7441 gcacccatat aaatacggca tttcaggccg agcagggcgc tggcaagggc cgacgccacg
     7501 ccatgctgac cggccccggt ttcggcgatg atttcggttt tacccatccg cttcgccagc
     7561 aacgcctgcc ccagcacctg gttggtttta tgtgcgccgc cgtgcagcaa atcttcacgc
     7621 ttgagataca gcgtggtgtt cgtcccggct gtaatgttct ggcatttggt cagtgcggtt
     7681 ggacgcccgg catagttttt cagcaggtcg ttgaactggg cttgaaattc aggatctttt
     7741 tgcgcgctga caaaagcttc ttccagctgg cgcagagcag gcatcaggat ttgtggcacg
     7801 tacatgccgc caaactcacc aaaatagggg ttaagtaatg ttgtcattgt tcctttcctt
     7861 aatatgcgcg cagcgtctgg aaaaccgagg ccaaaagacg tgcgtctttg atgcccggtt
     7921 gcgactctac agcagaatta aaatcaagtc cggcgcagcc ggtttgtgcc gcttccacgc
     7981 agttatctgc gcctaagccc cccgccagca gaacgttgcc aagcgattga ccatttaata
     8041 gtgaccagtc aaaacgttgc ccgcttccac cctggccgtt gtctaaaaca tatttatcaa
     8101 cgtgctgaaa ctcgcgggcg ggcagggttt caccgacgct taatgctttc cagatggcaa
     8161 catgtgctgg cagagcttca cgcagcgtat cgatatacag ctgatcttca ttgccatgca
     8221 gttgcactgc cgccagcgat aacaccttag ctttgtccac cacatcggca atatcgtgat
     8281 tgcggaacac gccaacatac tgcaacggtg ccgcagccat cacttcctgc gcctgttcaa
     8341 cgttgacgca acgcggtgat gtcgcaacaa aaatcaaccc accgtaaatc gcgcccgcgt
     8401 cataagctgc tttagcatct tgcccacgcg tcaggccaca tactttattc tcacccagca
     8461 acacccggcg cacggcggcg tgcaaatcgt catgggccat caacgccgaa ccaatcagaa
     8521 aaccgttagc gaagtggctt aactcgcgca cctgagcgta agtattgatg ccggattcgc
     8581 tgattaccgt tacgttgtgc cccagtttcg gcgcaagctc gcgggtacgg ttgagatcaa
     8641 tcgacaaatc acgcagatcg cggttgttga tgccaacgac ctttgcccct aatgcaatgg
     8701 cgcgctccag ttcctcttca ttactgactt cggtcagcac ccccatctcc agactgtgag
     8761 cgacggctgc aagctggcga tattggtcgt catccagtac tgaaagcatt aataagcagg
     8821 catcggcctg gtaatagcgc gccagataga tctggtaagg gtcgataatg aagtctttac
     8881 ataaaatcgg ctgcggggcg atttggctga cgatgaggag gaaatcaaag ctcccctgaa
     8941 aatatttctc atcagtcagc accgaaattg ccgaagcgta atgtttataa atggcggcaa
     9001 tgcgtgccgg atcgaaatca tcacggatca cgccttttga cggcgacgct tttttacact
     9061 ccagaataaa cgccgtgcgt gcaccctgta gcgcatcata aaaatgtcgt gtgctcggct
     9121 gaacctcatt ctgaaaactg gccagcggtt gctgctgttt gcgggtttct acccaaatcg
     9181 ccttgtctgc gacgattttc gctaaaacgg tttgcatcat ttaccctcgt gccgccagcg
     9241 cggtaactct gtcgtaagcg gaaccactgc gcagtacctc aagaacggtt tgcgcattgg
     9301 cttgcagatc ttcatggcca tgcaggcgca ttaacatggc gacgttcgca gcgacggctg
     9361 cttcatgggc ggcgtcgcct ttaccttgta acaagcgtgt taaaatgtca cggttttctt
     9421 caggtgttcc gcctgccagt tgctcctggt ggtagggagt caggccaaaa tcttcagcgg
     9481 tcaattgata gctcttaatt ttgccgtcat gcagctcggc aacgattgtc ggcgcgtgta
     9541 atgaaacttc atccatcccg ccgctgtgca ccaccgccgc gcgttgatac cccagcacgc
     9601 gcaaggtttc ggcaatcggc agcaccagtt ccggactata aacaccaatt aacgccagcg
     9661 gcggatgcgc cgggttaatc aatggcccca gcacattgaa cagggtgcgg gttttcagtt
     9721 gctggcgaac cggcatcgcg tggcggaatc cggtgtgata tttcggcgca aagaggaaac
     9781 atacgcctaa ctcgtccagc gcctggcgcg atttattggc gttcatatca agattaatac
     9841 cgaatgccgc cagcagatcc gacgagccgg atttactgga gacgctgcgg ttaccgtgtt
     9901 tcgccacttt cagcccacag gccgcggcga caaacgcact ggcggtagaa atattgatac
     9961 tgttgctgcc gtcaccgcca gtaccgacga tatcagcaaa cagataatcc gggcgcggga
    10021 acggcgctgc gttttccagt agcgcggttg ctgccccggc gatctcgttc gggtgctcac
    10081 cgcgaatttt catgctcacc agcgccgccg ccagttgttc cggcttcagc tcgccacgca
    10141 ccaccgctga aaacagctgg tggctttctt gttggctaag cgtctgcgcc tgatacagtt
    10201 tttccagaat cggttgcagc gtgttggctg gctctagttt ctgctgcgcc caggccagcg
    10261 tttgttccag caggcgagcg ccctgggtgg tgagaatgga ttccggatgg aactggaatc
    10321 cacaaacgcg atccgcatcg tgacgtactg ccatcaccat gccattaaaa tgggcgttga
    10381 tggttaaacc ggccggaatg ttactgccaa ccagcgagtg ataacgcgcc accggcagcg
    10441 ggtttgttaa tccggcaaac atcgcctgac cgtcatgttc aatgctcgac gctttaccgt
    10501 gaagaatttc gcccgcctga ccgacatagc ccccgtaagc ttcgacaatt gcctgatgtc
    10561 cgaggcaaat gccaataatg ggcagcttgc cacgcaagcg ggtgaggagt tccggcatac
    10621 aaccggcttc gctcggcaca ccggggccag gagaaagcat cagtaccgga ttgctcatcg
    10681 tcgccaggcg ttcaattaag gtctgcgccg gaatatggtt gcggtaaatc accacgttat
    10741 gaccattgct gcgcaactga tctgccaggt tgtacgtaaa agagtcgata ttatcgagca
    10801 gcagaatgtc agccatcaga aagtctcctg tgcatgatgc gcggtggcaa tagcgcgcag
    10861 tacagcgcgg gctttgttac gggtttcgtc ggcttccgac tgcggaacag aatcaaggac
    10921 tacaccagca cccgcttgca cggtggcgat accgttttcc accagcgccg agcggatcac
    10981 aatgcaggtg tcgagatcgc catgcgcggt gaaataacct accgcgccgc cgtagctgcc
    11041 gcggcgacga ccttccgcct cggcaattaa ctgcatagcg cgtactttcg gcgcaccgct
    11101 taacgtcccc atattcatac aggcgcgata agcgtgcagg gcgtcaagat cgtgacgcag
    11161 ttcgccgact acgcgagaga cgaggtgcat cacataggaa taacggtcaa ctttggtgag
    11221 atcggcgatg tagcggctgc caggggtgca aatgcgtgcc agatcattac gggcgagatc
    11281 aaccagcatc agatgttcag acagctcttt atggtcagta cgcatttcca gttcaatacg
    11341 gctgtcgaga tctctgtcca gtgaaccatc ggcgcgacga ccgcgtgggc gtgttccggc
    11401 aatcgggtag atctcaatct ggcggctggt ggcatcatac ttgagcgagc tttccggcga
    11461 cgcgccaaat agggtgaaat cattatcctg cataaaaaac atgtacgggc tgggattact
    11521 ctttttcagc acgtaatagg ccgccagcgg tgacgggcag ggcagagaga aacggcgaga
    11581 tggcaccacc tggaaaattt ctccagcgcg aatcgctttt tgcaacaaac gcactacgcc
    11641 accgaactct tcatcgctct gattacattc acaacgcata tgcggcacgg aaaccactgg
    11701 cagcggcggc gcggcttcgg tcagttgctg acgtagttcg ttcaggcgag cagtgagacg
    11761 ttgtttttct tcttcattcg gagcaaacag gctggcctga atacgggtgc tttttttctg
    11821 atggtcaatc accatcagcg tttcagcgag ataaaaacag aaatcagggc agttattttc
    11881 cgctgacagt tgcggtaaat cttcaaatcc cgccacaagg tcataagaga acaggccgcc
    11941 gaagaacatt gcttctcgtt cttccttcgg tacattcaac agattctgca ataaacggaa
    12001 agcgtcaaaa accgaaaggg agcataagcg ggcgtcttca tccagcagtg gactgacagg
    12061 ggggaagcgc agcacacggc agtttggtga ttgttcactt tccacacccg caggcagggc
    12121 gttatccagt agtgccagca gggcttcgcc attgccggaa agtgcctgaa ttgtgacggt
    12181 gtcacctaat gctgtaatgc gcagcgcact gtctatcagc agcaggcttt ttaaatcatc
    12241 tttgctgtcg atatctgcgg attccagcag cagcgttgcc ggacgattcc cacacaactg
    12301 gtgaaaaagc gcagtcgggt tgtcgcgata agcgccttcg caggttagca gttcgagagt
    12361 cggtttttgt gtttgcattg ttattctcta attttgttca aaaaaaagcc cgctcattag
    12421 gcgggctggg tatctgattg ctttacgcat ggtgaataca ctgcccgttt caggaagtgc
    12481 gccaccaacc tttcagtacg aaaattgctt tcattgtcga tacccttttt acgtgaactt
    12541 gcgtactagt taactggttc gatgattaat tgtcaacagc tcatttcaga atatttgcca
    12601 gacccgttat gatgtcggcg caaaaaacat tatccagaac gggagtgcgc cttgagcgac
    12661 acgaattatg cagtgattta cgacctgcac agccatacca cagcttccga tggctgcctg
    12721 acgccagaag cattggtgca ccgtgcagtc gagatgcgcg tcggcaccct ggcgatcacc
    12781 gaccatgaca ccacagctgc aatcgcgcca gcaagagagg aaatttcacg ttcgggactg
    12841 gcgctgaatc ttattcccgg cgtggaaatt tccacggtct gggaaaatca tgaaattcat
    12901 attgtcgggc tgaatattga tattactcat ccgctaatgt gtgagttcct cgcgcagcag
    12961 acagaacggc gcaatcagcg ggcgcagcaa attgccgaaa gacttgaaaa agcgcaaata
    13021 cctggggcgc tggaaggcgc acaacgactg gcgcagggcg gcgcagtgac gcgcggtcat
    13081 tttgcgcgtt tccttgtgga gtgcggcaaa gccagttcaa tggcagatgt ctttaaaaag
    13141 tatctggcgc gcgggaaaac cggatacgtt ccgccacagt ggtgtacaat agaacaagct
    13201 attgatgtca ttcatcattc tggcggtaag gcggtgctag ctcatcctgg gcggtacaat
    13261 cttagtgcta aatggctgaa aagattggtt gcgcattttg ccgaacacca cggcgacgcg
    13321 atggaagtcg cgcagtgcca gcaatcgccc aatgaacgca cccagctggc cacacttgcg
    13381 cgtcagcatc atttatgggc atcacaagga tctgattttc atcagccatg cccgtggatc
    13441 gaactgggtc gtaaactctg gctgcccgca ggcgttgaag gcgtctggca gctatgggaa
    13501 cagccgcaga acaccacaga gagggaatta tgagccagtt tttttatatt catcctgata
    13561 acccgcagca acgtctgatc aaccaggcgg tggagatcgt gcgtaaaggc ggggtgattg
    13621 tctatccaac tgattccggc tatgcgctcg gctgtaaaat tgaagataaa aacgccatgg
    13681 agcgtatttg tcgtattcgc cagctgccgg acggtcacaa ctttaccctg atgtgtcgcg
    13741 atctttctga actctcgacc tattcattcg tggataacgt tgcgtttcgt ttgatgaaaa
    13801 acaacacgcc gggcaactat accttcatcc tgaaggggac gaaggaagtg ccacgccgtc
    13861 tgttgcagga gaaacgcaaa accatcggta tgcgtgtgcc ttctaacccg attgcgcagg
    13921 cgttgcttga agcactgggc gaaccgatgc tttccacttc gctaatgctg ccaggcagcg
    13981 aatttaccga atccgatccc gaagaaatta aagaccgtct ggaaaaacag gtagatttga
    14041 ttattcacgg cggctatctc gggcagaaac caacaacggt tattgatctt accgatgata
    14101 cgccggtcgt ggtgcgtgaa ggcgtaggtg atgtgaagcc tttcttataa gtgcgtaact
    14161 ctgtatacta cgcggcctta aaatggcgct atacggcgcc attgattctc ctgttcgacg
    14221 cctgtgaagg cgacaccaaa aggaagctct atgagcgaaa aattacagaa agtgctggcc
    14281 cgcgctggcc acggctcccg ccgtgaaatc gaatctatta ttgaagccgg tcgtgtgagt
    14341 gttgatggca aaattgctaa actcggcgat cgcgttgaag ttacccctgg gctgaaaatt
    14401 cgtatcgatg gtcatctgat ttcggtacgt gagtccgctg aacaaatttg tcgcgttctg
    14461 gcctattaca aaccggaagg tgagttgtgt acccgtaacg atccggaagg gcgtccgacg
    14521 gtgtttgacc gtctgccaaa gctgcgtggc gcacgctgga ttgccgtagg tcgtctggac
    14581 gttaacacct gtggtctgct gctgttcacc accgatggtg aactggcaaa ccgtttaatg
    14641 catccaagcc gtgaagttga acgtgaatac gccgtgcgtg tatttggtca ggttgacgac
    14701 gcgaaactgc gtgatttgag tcgtggcgtg caactggaag atggtccggc agcttttaaa
    14761 accatcaagt tcagcggcgg cgaagggatc aaccagtggt acaacgtgac tctgaccgaa
    14821 ggccgtaacc gtgaagttcg tcgtctgtgg gaagcggttg gtgtgcaggt gagccgcctg
    14881 atccgtgttc gttacggtga tatcccactg ccaaaaggtc tgccacgcgg tggctggacg
    14941 gagctggacc tcgcccagac taactatctg cgcgaactgg tggagcttcc gccagaaacc
    15001 agctctaaag tcgctgtaga aaaagaccgt cgtcgcatga aggctaatca gattcgtcgt
    15061 gcggtgaaac gtcacagtca ggtgagcggc agtcgtcgtt ctggcgggcg taataacggt
    15121 taatcaaagt atgccggacg tcatatccgg catttttaca gattaataat caatccctat
    15181 ttgcgcctta acaccggcat caaacgcatg tctgatgggg cgcagctcac ttaccgtatc
    15241 cgccagttca agaatatccc gatgacagcc tcgacccgtg ataatcaccg tctgttgatg
    15301 tggacgttca tttaacgcct gcaccacttc ttccagtggc aaatagtcat acgccaccat
    15361 atacgtcagt tcatcaagca aaaccatatc cagtgaggaa tcagcaagca tccgctttgc
    15421 atgttgccag acttcgcggc aggcggcggt atcagactcg cggttttgtg tatcccaggt
    15481 aaagcccgtt gccatcacct gaaactcaac gccatgtggc tccagcagat tgcgttcgcc
    15541 attaggccag gtgcctttaa taaactgcac gacgcccact ttttttccgt gaccaactgc
    15601 gcgtgttgcc gtaccaaatg ccgcggtggt tttgcctttt ccattgccgg taaagacgat
    15661 gataataccg cgttcatcct gggcctgggc cacacgagca tctacttttt ctttcactcg
    15721 ctgctgacgc tgttggtagc gttcatcact cattgggaaa ttcctggttt acggcccggt
    15781 tgggcgtcaa aggtcatgcc ggttttacgg cggctgtcat cgcccatcag ccagaggtag
    15841 agcggcatga tatcagcggg tgttttaagt ttctgtggat cttcggtcgg gaaggcgctt
    15901 gcgcgcattg cggtgcgcgt accgcctggg ttaatgcagt tgacgcgcag gcgctgctga
    15961 tattcatcgg ccagtacctg catcatccct tcggtggcaa atttcgacgc tgcatatgca
    16021 ccccagttgg ctcgtccctg acgtccaacg cttgatgaag taaagaccag tgaaccggcg
    16081 tccgatttga gtaataaagg aagcagtgcc tgggtgagca taaaggtggc attaacgttg
    16141 atctgcatga cgtcctgcca gacctgcgga ttttgttcgc tcattgggca aacatcgccg
    16201 agcaatccgg cattatgcaa aacaccatcc agacgcggat aattaacgac aatgcgctgt
    16261 gccagttgtt ggcaattttc ggacgtgcag gtcagcaaat cgagaataaa ccactgtggc
    16321 tgacgcccag tttcttcgtt tatgtggctg gctacctgac gtaatttttc ttcattacgg
    16381 cccaacagaa tcactgtcgc accatagcgt gcatacgtca tcgcggcttc acgaccaatc
    16441 ccatcgctgg ctcccgtcac caggataatg cgatcattga gtaaatcttg ttttggctgg
    16501 taatgcatgg ctactcctca acgacgttgt ctgtcgcgct gccagtgtac tttatgactt
    16561 tggggcttta tgcctgaaac agaagggcat ttcaatcagc ctggggcgat aacaccgcaa
    16621 aatggatact ttgtcatact ttcgctgcaa taacatctct gcgagacggc ttaacatgcc
    16681 tgttgtaaac tgtgagccaa agcgttgttt aaccaaggtg gggactcgtg gaattgttgt
    16741 ctgaatatgg tttgtttttg gctaaaatcg ttaccgttgt gctagcgatt gcggcgattg
    16801 ccgccattat tgtcaatgtt gctcaacgta ataaacgcca gcgtggcgag ttacgggtca
    16861 acaatctcag cgaacagtat aaggagatga aagaagaact ggccgcggcg ctgatggact
    16921 cacatcagca aaaacagtgg cacaaagcgc agaagaaaaa gcacaagcaa gaagcgaaag
    16981 cagcaaaagc gaaagccaaa ctgggcgagg tggcaactga cagtaaacct cgcgtctggg
    17041 tgctggattt taaaggcagc atggacgccc atgaagtgaa ctcgctacgt gaagagataa
    17101 cggcggtact cgcagcattc aaaccgcagg atcaggttgt gctccgtctg gaaagccctg
    17161 gtggcatggt gcatggttac gggttggcgg cttcgcagct gcagcgtctg cgtgataaaa
    17221 acattccttt aactgttacg gtagacaaag tcgctgccag cggcggttac atgatggcct
    17281 gtgtggcgga caaaattgtt tccgcaccgt ttgctattgt gggttccatt ggggtggtgg
    17341 cgcaaatgcc caactttaac cgcttcctga aaagcaaaga tattgatatc gaactgcaca
    17401 ccgccgggca gtataagcgc acgctgacct tgctgggtga aaataccgaa gaagggcggg
    17461 agaaattccg cgaagagctg aacgaaacgc atcagttatt taaagatttt gtgaagcgta
    17521 tgcgtccgtc tctggatatt gaacaggtgg caacgggtga acactggtac ggacaacagg
    17581 cggtagagaa aggcctggtt gatgaaatca acaccagtga tgaagttatt cttagcctga
    17641 tggaaggccg tgaagtggtt aatgtacgct atatgcagcg taaacgactc attgaccgat
    17701 tcaccggcag cgcggcagag agcgccgatc gattgttgct acgctggtgg cagcggggtc
    17761 aaaagccatt gatgtaaaag acaaacgcga ggctaagacc tcgcgttttg ctttaatcaa
    17821 ccagatgata tttttctgaa agcacatggg ccaggtgttt gaacatatta aacaccgcgg
    17881 tgcttttggc tgttggcaat ccttgttcat ctaaaaagta gtcgccggta aataccagca
    17941 cgccattacg ctgcgtgacg ccggtggctt caatccctgc gagcgtatcc tcatgctcac
    18001 gaatgatttt gttggcctct ttcaacagcg tttcgcggtc gatgggttgt gtctctttgt
    18061 tcattattta ctccttaaac aaggacatta gtctacgcca ggcatggctt gcagacaaat
    18121 ataccacgct ggtggcaaga gcgccttact ggcaactttg gattttgcat gctaataaag
    18181 ttgcgtatcg gattttatca ggtacagtgt gacgctttcg tcaatctggc aatagatttg
    18241 cttgacattc gaccaaaaat ccgtcgtgct atagcgcctg taggccaaga cctgttaact
    18301 cagtcacctg aattttcgtg aacagagtca cgacaagggg ttgatatccg cagagagcga
    18361 gtccatatcg gtaactcgtt gccagtggaa ggtttatcaa cgtgcgacgc attcctggaa
    18421 gaatcaaatt aggtaaggtg aatatgggta aagctcttgt catcgttgag tccccggcaa
    18481 aagccaaaac gatcaacaag tatctgggtt gtgactacgt ggtgaaatcc agcgtcggtc
    18541 acatccgcga tttgccgacc agtggctcag ctgccaaaaa gagtgccgac tctacctcca
    18601 ccaagacggc taaaaagcct aaaaaggatg aacgtggcgc tctcgtcaac cgtatggggg
    18661 ttgacccgtg gcacaattgg gaggcgcact atgaagtgtt gcctggtaaa gagaaggtcg
    18721 tctctgaact gaaacaactg gctgaaaaag ccgaccacat ctatctcgca accgaccttg
    18781 accgcgaagg ggaagccatt gcatggcacc tgcgggaagt gattggtggt gatgatgcgc
    18841 gctatagccg agtggtgttt aacgaaatta ctaaaaacgc gatccgccag gcatttaaca
    18901 aaccgggtga gctgaatatt gatcgtgtta atgcccagca ggcgcgtcgc tttatggacc
    18961 gcgtggtggg gtatatggtt tcgccgctgc tgtggaaaaa gatcgctcgt ggtctgtctg
    19021 ccggtcgtgt gcagtcggtg gcggtccgcc tggtggtaga gcgtgagcgt gaaattaaag
    19081 cgttcgtacc ggaagagttc tgggaagtcg atgccagcac gactacgcca tctggtgaag
    19141 cgttggagtt gcaggtgact catcagaatg acaaaccgtt ccgtccggtc aacaaagaac
    19201 aaactcaggc tgcggtaagt ctgctggaaa aagcgcgcta cagcgtgctg gaacgtgaag
    19261 acaaaccgac aaccagtaaa cctggcgctc cttttattac ctctacgctg caacaagctg
    19321 ccagcacccg tcttggtttt ggcgtgaaaa aaaccatgat gatggcgcag cgtttgtatg
    19381 aagcaggcta tatcacttac atgcgtaccg actccactaa cctgagtcag gacgcggtaa
    19441 atatggttcg cggttatatc agcgataatt ttggtaagaa atatctgccg gaaagtccga
    19501 atcagtacgc cagcaaagaa aactcacagg aagcgcacga agcgattcgt ccttctgacg
    19561 tcaatgtgat ggcggaatcg ctgaaggata tggaagcaga tgcgcagaaa ctgtaccagt
    19621 taatctggcg tcagttcgtt gcctgccaga tgaccccagc gaaatatgac tccacgacgc
    19681 tgaccgttgg tgcgggcgat ttccgcctga aagcacgcgg tcgtattttg cgctttgatg
    19741 gctggacaaa agtgatgcct gcactgcgta aaggcgatga agatcgtatc ttaccggcag
    19801 tcgataaagg cgatgctctg acgctcgttg aactaacacc agcccagcac tttaccaagc
    19861 cgccagcccg tttcagtgaa gcatcgctgg ttaaagaact ggaaaaacgt ggtatcggtc
    19921 gtccgtctac ctatgcgtcg atcatttcga ccattcagga tcgtggctat gtgcgagtag
    19981 aaaatcgtcg tttctatgcg gaaaaaatgg gcgaaatcgt caccgatcgc cttgaagaaa
    20041 atttccgcga gttaatgaac tacgacttta ccgcgcagat ggaaaacagc ctcgaccagg
    20101 tggcaaatca cgaagcagag tggaaagctg tactggatca cttcttctcg gatttcaccc
    20161 agcagttaga taaagctgaa aaagatccgg aagagggtgg tatgcgcccg aaccagatgg
    20221 ttctgaccag cattgactgc ccgacttgtg gtcgcaaaat ggggattcgc acagcgagta
    20281 ccggggtatt ccttggctgt tctggctatg cgctgccgcc gaaagagcgt tgcaaaacca
    20341 ccattaacct ggtgccggaa aacgaagtgc tgaacgtgct ggaaggcgaa gatgctgaaa
    20401 ccaacgcgct gcgcgcaaaa cgtcgttgcc cgaaatgcgg cacggcgatg gacagctatc
    20461 tcatcgatcc gaaacgtaag ttgcatgtct gtggtaataa cccaacctgc gacggttacg
    20521 agatcgaaga gggcgaattc cgcattaaag gttatgacgg cccgatcgtt gagtgtgaaa
    20581 aatgtggctc tgaaatgcac ctgaaaatgg ggcgtttcgg taaatacatg gcctgcacca
    20641 acgaagagtg taaaaacaca cgtaagattt tacgtaacgg tgaagtggca ccaccgaaag
    20701 aagatccggt gccattacct gagctgccgt gcgaaaaatc agatgcttat ttcgtgctgc
    20761 gtgacggtgc tgccggtgtg ttcctggctg ccaacacttt cccgaaatcg cgtgaaacgc
    20821 gtgcgccact ggtggaagag ctttatcgct tccgcgaccg tctgccggaa aaactgcgtt
    20881 atctggccga tgcgccacag caggatccgg aaggtaataa gaccatggtt cgctttagcc
    20941 gtaaaaccaa acagcaatat gtctcttcgg aaaaagacgg aaaggcgact ggctggtcag
    21001 cattttatgt tgatggcaaa tgggttgaag gaaaaaaata acctttaatt ctgtcaggtt
    21061 tttataaaca aagggtcgcg aaagcggccc ttttttattg catattattt tttcttcaca
    21121 cctatacact aaggctataa atgatatagt ggttatagtt agcacctttt ttattattaa
    21181 atcgtattag tcacccgcca ggtgtgacga aaaaacgatg ttctgatggc gtctaagtgg
    21241 atggtttaac atgaaattac aacaacttcg ctatattgtt gaggtggtca atcataacct
    21301 gaatgtctca tcaacagcgg aaggacttta cacatcacaa cccgggatca gtaaacaagt
    21361 cagaatgctg gaagacgagc taggcattca aattttttcc cgaagcggca agcacctgac
    21421 gcaggtaacg ccagcaggac aagaaataat tcgtatcgct cgcgaagtcc tgtcgaaagt
    21481 cgatgccata aaatcggttg ccggtgagca cacctggccg gataaaggct cgctgtatat
    21541 cgccaccacg catacccagg cacgctacgc gttaccaaac gttatcaaag gctttattga
    21601 gcgttatccc cgcgtttctt tgcatatgca ccagggcccg ccgacacaaa ttgctgatgc
    21661 cgtctctaaa ggcaatgccg atttcgctat cgccacagaa gcgctgcatc tgtatgaaga
    21721 tttagtgatg ttaccgtgct accactggaa tcgggctatt gtagtcactc cggatcaccc
    21781 gctggcaggc aaaaaagcca ttaccattga agaactggcg caatatccgt tggtgacata
    21841 taccttcggc tttaccggac gttcagaact ggatactgcc tttaaccgcg cagggttaac
    21901 gccgcgtatc gttttcacgg caacggatgc tgacgtcatt aaaacttacg tccggttagg
    21961 gctgggggta ggggtcattg ccagcatggc ggtggatccg gtcgccgatc ccgaccttgt
    22021 gcgcgttgat gctcacgata tcttcagcca cagtacaacc aaaattggtt ttcgccgtag
    22081 tacttttttg cgcagttata tgtatgattt cattcagcgt tttgcaccgc atttaacgcg
    22141 tgatgtcgtt gatgcggctg tcgcattgcg ctccaatgaa gaaattgagg tcatgtttaa
    22201 agatataaaa ctgccggaaa aataatttct tgcgttattt tcggcacctt ttatgtagcg
    22261 aaggtgccgg aatatattct cttttgttac ttatttctat accgtgcaca atatttttat
    22321 ccctcatatt tatagggtaa attacctgta aagtagtgtc taattacccg cctcgcaaat
    22381 tccagttttc aaatatctat ttcaggtcaa aagattgaat tattcactgg agacgattcg
    22441 taaattcgct ggatttttga ctaaagtttc tttaggattt gtctcatcgt atgattaatt
    22501 acaccaatca ttcaacgtct tgctgataag tgatggcgat tatatgaggt tagcaatgcc
    22561 ttctggaaat caggaacccc gccgtgatcc tgaattaaaa cgtaaagcct ggctggcggt
    22621 ttttcttggt tctgcacttt tctgggtggt tgtcgcactg ctgatttgga aagtgtgggg
    22681 ataactatgg tcggtcagga gcaactggag tcgtcaccat tatgccagca tagtgacaat
    22741 gaaccagaaa cgaaacggga atgttccgtc gttattccag acgactggca actaacatcg
    22801 cagcagcaag cctttataga actgtttgct gaagatgatc agccgaaaca ataattatca
    22861 tcattcttat tacccatttt taatgaatta aagggctttt aatacaccgc agcaataaca
    22921 gcttgagtta tctcaacaca aaataataac cgttaagggt gtagcctatg atcaacacaa
    22981 atatgaaata ttggtcctgg atgggcgcgt tttctctgtc gatgctcttc tgggccgaac
    23041 tcctctggat cattactcac tgatccttga ccccgctgcg gcggggttgt catttgcttt
    23101 gccacaaggt ttctcctctt ttatcaattt gggttgttat caaatcgtta cgcgatgttt
    23161 gtgttatctt taatattcac cctgaagaga atcagggctt cgcaaccctg tcattaagga
    23221 ggagctatgt cgtcaaccct acgagaagcc agtaaggaca cgttgcaggc caaagataaa
    23281 acttaccact actacagcct gccgcttgct gctaaatcac tgggcgatat cacccgtcta
    23341 cccaagtcac tcaaagtttt gctcgaaaac ctgctgcgct ggcaggatgg taactcagtt
    23401 accgaagagg atatccacgc actggcggga tggctgaaaa atgcccatgc tgaccgtgaa
    23461 attgcctacc gcccggcaag ggtgctgatg caggacttta ccggcgtacc tgccgttgtt
    23521 gatctggcgg caatgcgcga agcggttaaa cgcctcggtg gcgatactgc aaaggttaac
    23581 ccgctctcac cggtcgacct ggtcattgac cactcggtga ccgtcgatcg ttttggtgat
    23641 gatgaggcat ttgaagaaaa cgtacgcctg gaaatggagc gcaaccacga acgttatgtg
    23701 ttcctgaaat ggggaaagca agcgttcagt cggtttagcg tcgtgccgcc aggcacaggc
    23761 atttgccatc aggttaacct cgaatatctc ggcaaagcag tgtggagcga attgcaggac
    23821 ggtgaatgga ttgcttatcc ggatacactc gttggtactg actcgcacac cactatgatc
    23881 a
//
LOCUS       DAZSIO010000049        23204 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZSIO010000049 DAZSIO010000000
VERSION     DAZSIO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.049"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            221..328
                     /locus_tag="ACHX5T_001790"
     CDS             221..328
                     /locus_tag="ACHX5T_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR8467023.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            704..811
                     /locus_tag="ACHX5T_001791"
     CDS             704..811
                     /locus_tag="ACHX5T_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR8467024.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(898..2577)
                     /gene="bcsG"
                     /locus_tag="ACHX5T_001792"
     CDS             complement(898..2577)
                     /gene="bcsG"
                     /locus_tag="ACHX5T_001792"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR8467025.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2574..2765)
                     /gene="bcsF"
                     /locus_tag="ACHX5T_001793"
     CDS             complement(2574..2765)
                     /gene="bcsF"
                     /locus_tag="ACHX5T_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR8467026.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2762..4333)
                     /gene="bcsE"
                     /locus_tag="ACHX5T_001794"
     CDS             complement(2762..4333)
                     /gene="bcsE"
                     /locus_tag="ACHX5T_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR8467027.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            4606..4794
                     /gene="bcsR"
                     /locus_tag="ACHX5T_001795"
     CDS             4606..4794
                     /gene="bcsR"
                     /locus_tag="ACHX5T_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR8467028.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4806..5558
                     /gene="bcsQ"
                     /locus_tag="ACHX5T_001796"
     CDS             4806..5558
                     /gene="bcsQ"
                     /locus_tag="ACHX5T_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR8467029.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            5555..8172
                     /gene="bcsA"
                     /locus_tag="ACHX5T_001797"
                     /pseudo
     CDS             5555..8172
                     /gene="bcsA"
                     /locus_tag="ACHX5T_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            8183..10522
                     /gene="bcsB"
                     /locus_tag="ACHX5T_001798"
     CDS             8183..10522
                     /gene="bcsB"
                     /locus_tag="ACHX5T_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR8467030.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGTPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            10529..11635
                     /gene="bcsZ"
                     /locus_tag="ACHX5T_001799"
     CDS             10529..11635
                     /gene="bcsZ"
                     /locus_tag="ACHX5T_001799"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR8467031.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            11830..12219
                     /locus_tag="ACHX5T_001800"
     CDS             11830..12219
                     /locus_tag="ACHX5T_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001391818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase subunit BcsC-related outer
                     membrane protein"
                     /protein_id="HFR8467032.1"
                     /translation="MISNAACKTTVWHSRQRYWKTRANGRRRQHFSGNDWRWAPAAYG
                     LLTDWQEEAARQSNDGGSSQGFGYTARALLERRVTSNWFVGTAIDIQQVKDYAPSHFL
                     LYVRYSAAGWQGDMDLPPQPLIPYADW"
     gene            12301..14289
                     /gene="hmsP"
                     /locus_tag="ACHX5T_001801"
     CDS             12301..14289
                     /gene="hmsP"
                     /locus_tag="ACHX5T_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR8467033.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            14479..15765
                     /gene="dctA"
                     /locus_tag="ACHX5T_001802"
     CDS             14479..15765
                     /gene="dctA"
                     /locus_tag="ACHX5T_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR8467034.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            15986..17482
                     /locus_tag="ACHX5T_001803"
     CDS             15986..17482
                     /locus_tag="ACHX5T_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR8467035.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(17578..18507)
                     /gene="kdgK"
                     /locus_tag="ACHX5T_001804"
     CDS             complement(17578..18507)
                     /gene="kdgK"
                     /locus_tag="ACHX5T_001804"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR8467036.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            18739..19506
                     /gene="pdeH"
                     /locus_tag="ACHX5T_001805"
     CDS             18739..19506
                     /gene="pdeH"
                     /locus_tag="ACHX5T_001805"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR8467037.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            19576..21636
                     /locus_tag="ACHX5T_001806"
     CDS             19576..21636
                     /locus_tag="ACHX5T_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR8467038.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(21818..23140)
                     /locus_tag="ACHX5T_001807"
     CDS             complement(21818..23140)
                     /locus_tag="ACHX5T_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8467039.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
BASE COUNT         5477 a         5892 c         6383 g         5452 t
ORIGIN      
        1 ttccggaaca gaaaaacagt cgtagaatca gcgtcaagcc ggaagggttc cggtgaggcg
       61 caatgttgcg ggggctttat ccctggtggc attggttgct ggagagagaa aacccccgca
      121 cgttgcaggt atgcacctga caacatcacg ggggccattc ttgaacctga acaactcaag
      181 aatagccgcg aaacgttgtc attacaacac aggcggctat atgacgctcg cagaactggg
      241 catggccttc tggcatgatt tagcggctcc ggtcattgct ggcattcttg ccagtatgat
      301 cgtgaactgg ctgaacaagc ggaagtaacg tgtcatgcgg gcgtcaggct gccgtaatgg
      361 caatttgcgc ccggaccagg ccgcaggggt gaaactctgc ggcctttttc gtaagaaaaa
      421 tgcaacgatt gcacgcggat aatattctgc ggaatgcgca gcacagagtg aagacgtgac
      481 ggattccgga acagaaaaac agtcgtagaa tcagcggcaa gccggaaagg ttccggtgag
      541 gcgcaatgtt gcgggggctt tatccctggt ggcattggtt gctggagaga gaaaaccccc
      601 gcacgttgca ggtatgcacc tgacaacatc acgggggcta atcttgaacc caaacaactc
      661 aagaatagcc gcgaaacgtt ggcattacaa cacaggcggc tatatgacgc tcgcagaact
      721 gggcatggcc ttctggcatg atttagcggc tccggtcatt gctggcattc ttgccagtat
      781 gatcgtgaac tggctgaata agcggaagta acgtgtcatg cgggcgtcag gctgccgtaa
      841 tggcaatttg cgcccggatc aggccgcaga ggttaaactc tgcggccttt ttcgtgctta
      901 ctgcgggtaa ggcacccagt cgccgccgtt caggcgaacg tacggtttat cttggtattg
      961 aataactact gcatttgagt tctcggagac cggtgctgtt tgtggcaacc cactggtgag
     1021 ttttttccag tcaacattgt cttcggtgaa aatcttgcca tcaagaacgc gaaccaccag
     1081 atcggagata gccaggaagc tgctcggttg gtcaatgaca atcggtgccc cctgatgtgg
     1141 tgccttcatg ccgaagaatt tcaccccaac ggggacgtct gtgatagacg ggctagggat
     1201 atcacgtagg ccagatacct gcattctgtc gcccttcagc gcgccgccgt gttccggcac
     1261 cacgaccacc atcactttac gacccgattt ctccagttca gtaaagaagg cgtccagttc
     1321 atcaaagaat ttctgcgccc gcgctttgta atccgctgtt ttgctgaccc ccggataatg
     1381 gttgccgtca tgcagtggaa gcgtgttgta gaacgtggca ctacggctgt ttttatcttt
     1441 ttcggtaacg tccagccagc ggttaagcac ggcggtatcg tcataaaccg gcgaaccatc
     1501 aaagcccagc aaaataaccg gcagatttgt ttgatccatc aattcagtct gcatgccgcc
     1561 attttcgcga acttctttca aaaaaccgcc gaactggccg ttatgtccca tcatcaggtg
     1621 ctgggtaaag cccagtttcg aaaggttatc aaacagatag cagtcgttat ttgccggttg
     1681 atacagatta gtgtgcgaag tctgcccgca gctggcgcgc agtaaacgga tcgccgccgg
     1741 gccactgtag gaggtggcgg agttaaagtt cttgaaatca atatcgaaat gcgaccacag
     1801 tggatgcgac atcaacccgg cggcttctat atccgaccag gaaagcgaac agatgttaat
     1861 caccagtagt tcaaatggct gagcatcagc gggcagcgaa gacgggaagg tcgatttacg
     1921 tttcgcctcc gcgttataga aattattcag ccaggcgtta aggttcgccg ttgttggcgg
     1981 tgcagtttgt gcgggcatat cacccactac cggtgcgcca cccgtcgccg caacggttgc
     2041 cgctgcgtta ccacccgtcg ttgttacagt ggtcgtcggt tgtccggctg gccacaagga
     2101 gaaacttggt cccgccaggg taagtacgtt cagccatagc agtatggcaa ccacaaaaac
     2161 ggtaatgcga atccattgtg acaggaataa ccaggccact aataaaacaa aaatggcccc
     2221 aatcatctgc cagttaataa agcgtgtgac aaggtcgatt aaataatcgg tactgaaccc
     2281 cgccacctgc gaaccctggc tcattatgct ttccgggcca ggcaaccaga tgtcatgcca
     2341 gaacaaagca aagccgatcg gcagggcaat ccagtggcgc aagcgatgca ggctgtaacg
     2401 cggaatgggc atcagcagaa acgcggcaaa caccaaattg aggagcgggt ggaagttaag
     2461 atatcccgcc cacaacaggc caaacttaac cagaaaatag aagttccagc cggaaaggcc
     2521 gcgccagtat tgccagaggg aagaaggcat ggcggtattt tgcgtaaatt gagtcatttt
     2581 ttggttgccc tggctttttc cgtgcggcgt aacgtcccgg ccggtttaac ataacgaggt
     2641 ttagcaaaga acaaacgtaa ggtgtcgcga atgcgtcgca aagagtgccg cgccagatag
     2701 cccagcggga aaaatatcag tgcgcaaacg ataataattt caatgatatc gctgatggtc
     2761 atcatgatga gcgctccaca gcatcatcta acagtcgcat gggttctggt attcgtcgcc
     2821 agtggcgacc atcgtgctcg gcattgatga ccggtttaga actttgcgtt aaaggcagcg
     2881 gcattcccca ttgttctggg gcaagcaggc gcatctgcac cagctcggca ctgatttgat
     2941 catcttcaaa ccagaccata cggtttgaga aaatgtcgcc agtaggcaat gggaaaatat
     3001 gattcaacgc ggtatccaga tcgttaatcc gacagaatga gagaaacagc accagccgat
     3061 taccgccaat ggtcatgata tcgccggtac ggttagggcg acatagcgtc agggcttgct
     3121 caacgcggat acccggtacc ggacgtaggg caaccagaac gcctttaccg tgggcaggca
     3181 agagagggtt attcatcatg ttgttgacgg cattacagaa cacatcccac ttctggaaac
     3241 cacgcagttt gagcggctgg gtcattgaca gcaaggtagt gatatcttcc ggtacatagc
     3301 gactaaactt ctgcccttgc acgctttcga tcatcgtcag acaacgggag agtggcgcat
     3361 tccacggaat aaccatattt gcaccgcagg ccaataacaa acgttcatcg gtggcgcgca
     3421 ggctagcggt attttcccgc acgaggattt tcatcgcact accgcgctgg cgacgcaggg
     3481 tatgaatgct gcgggccagt ggctcgattt gcgcattttg ctgtaaagaa aagaccaccg
     3541 tcgccgcctg agcggtacgg gcttcattga acaggacttc gttattgttg aacagttgcc
     3601 agtgttccga tagcggcggc gcaccttcca gtacagcaac attactcaga atgcgttttt
     3661 cgtcgctgcg tggttggatc tccgcctctt cgctttgaac taatgtccag ataccatttt
     3721 gttgctgaac gctaagctgc tgacgggcgc tgaccccttt ttcgttgcac cagaaggcaa
     3781 tatccagcaa atgttggtca ccctgaaaac gcaaactggc aagaccaaaa agtgaacggt
     3841 attcctcaag caacaatgaa aattgtttat cgttattatt tccgggatta attaccaaaa
     3901 gcgaacaatg gtttaacctg ctccatttat tcattttatc caaccatgag cgaagccgct
     3961 cggcaggaat attttgccat gcgttatttg cgcaaacaag aataaaaagg taattatggg
     4021 gatcaataga acactgcaaa tcacgggcca aatagtatag acctttttca tgatttagca
     4081 ttgaaaataa ttttattttt tccggccctt gagaatcatc taattgaaag attttcgccg
     4141 gatcgctgtc catgctaatg accgcgacgt gtgcggtttc agcctgggat gcaattgttt
     4201 gattcgccag actgatagca tcttcatggc gatcgacgtt aaaccaccag acgccgcctg
     4261 ctggcatatg tcgcagctca tcccataatg atgagatacc gatagagaat acagggtcca
     4321 caatgtccct catgtcacca ttttgtctga ttatcagttt actagcgaaa gctaagaaat
     4381 aaacctacca ttgaaattaa aacttatcag atttagcatg tgaatcaatt tagaaggtca
     4441 caagacgtgt gattttagtc ttttcgtttt taactttttc tgttgcgtaa cggaaagtca
     4501 aaaagtgagc aaattcccgt ctcgccgtaa acaataaacc ggcgaaatgc tcacggttag
     4561 aattgtctca tgaatggtac ggttatttca tagggatcaa gcaaaatgaa taacaatgaa
     4621 ccagatactc tgcctgatcc cgcgataggc tatatcttcc agaatgatat tgtggcgtta
     4681 aagcaggcat tttcactgcc tgatattgat tatgccgata tttcccaacg cgaacagttg
     4741 gccgcggcat taaaacgctg gccgttgctg gcagagtttg cgcaacaaaa gtaggggatt
     4801 ggtgaatggc cgtactggga ttgcaggggg tgcggggagg cgtggggaca acaaccatca
     4861 ccgccgcatt agcctggtca ttacaaatgt tgggagaaaa tgtcctggtg gtcgatgcct
     4921 gcccggacaa cttgttgcgc ctgtcattta atgttgattt tacccaccgt cagggctggg
     4981 ccagagcgat gctggatggc caggactggc gtgacgctgg gttgcgctac acctcgcagc
     5041 tcgatttgct gccttttggt cagttatcca ttgaagaaca agaaaatcca cagcactggc
     5101 aaacccggct gagcgatatt tgctccggct tacagcaact aaaagccagc gggcgttacc
     5161 agtggatttt aatcgactta ccgcgtgatg cctcgcagat aacccaccag ctgctgagtt
     5221 tgtgcgagca ctcgctggca atcgtcaatg tggatgccaa ctgccatatc cgactgcatc
     5281 agcaagcgct gccggatggc gcacatattt tgattaatga cttccgtatt ggcagtcagg
     5341 ttcaggacga tatttaccag ctttggttgc aaagccagcg ccgattactg ccgatgctca
     5401 ttcatcgtga tgaagcgatg gctgaatgcc tggcggctaa gcaaccagta ggtgaatatc
     5461 gcagtgatgc gctggcggct gaagagatac tgacgctggc gaactggtgc ctgttgaact
     5521 actccgggct gaaaacgcca gtcgggagtg catcatgagt atcctgaccc ggtggttgct
     5581 tatcccgccg gtcaacgcgc ggcttatcgg gcgttatcgc gattatcgtc gtcacggtgc
     5641 gtcggctttc agcgcgacgc tcggctgttt ctggatgatc ctggcctgga tttttattcc
     5701 gctggagcac ccgcgctggc agcgtattcg cgcagaacat aaaaacctgt atacgcatat
     5761 caacgcctcg cgtccgcgtc cgctggaccc ggtccgttat ctcattcaaa catgctggtt
     5821 attgatcggt gcatcgcgca aagaaacgcc gaaaccgcgc agcgggcatt ttcaggtctg
     5881 caaaatattc gtggacgtta ccatcaatgg atgaacgagc tgcctgagcg cgttagccat
     5941 aaaacacagc atctggatga aaaaaaagag ctcggtcatt tgagtgccgg ggcgcggcgg
     6001 ttgatcctcg gtatcatcgt caccttctcg ctgattctgg cgttaatctg cgttactcag
     6061 ccgtttaacc cgctggcgca gtttatcttc ctgatgctgc tgtggggggt agcgctgatc
     6121 gtacggcgga tgccggggcg cttctcggcg ctaatgttga ttgtgctgtc gctgaccgtt
     6181 tcttgccgtt atatctggtg gcgttacacc tctacgctga actgggacga tccggtcagc
     6241 ctggtgtgcg ggcttattct gctcttcgct gaaacgtacg cgtggattgt gctggtgctc
     6301 ggctacttcc aggtagtatg gccgctgaat cgtcagccgg tgccattgcc gaaggatatg
     6361 tcgctgtggc cgtcagtgga tatctttgtc ccgacttaca acgaagatct caatgtggtg
     6421 aaaaatacca tttacgcctc gctgggtatc gactggccga aagacaaact gaacatctgg
     6481 atcctcgatg acggcggcag ggaagagttt cgccagtttg cgcaaaacgt gggggtgaaa
     6541 tatatcgccc gcaccactca tgaacatgcg aaagcgggca acatcaacaa tgcgctgaaa
     6601 tatgccaaag gcgagttcgt gtcgattttc gactgcgacc acgtaccaac gcgatcgttc
     6661 ctgcaaatga ccatgggctg gttcctgaaa gagaaacagc tggcgatgat gcagactcca
     6721 caccacttct tctcgccgga cccgtttgaa cgcaacctgg gacgttttcg taaaacgccg
     6781 aacgaaggca cgctgttcta tggtctggtg caggatggca acgatatgtg ggacgccact
     6841 ttcttctgcg gttcctgtgc ggtgattcgt cgtaagccgc tggatgaaat tggcggcatt
     6901 gctgtcgaaa ccgtgactga agatgcgcat acttctctgc gtttgcaccg tcgtggctat
     6961 acctccgcgt atatgcgtat tccgcaggcg gcggggctgg cgaccgaaag tctgtcggcg
     7021 catatcggtc agcgtattcg ctgggcgcgc gggatggtat aaatcttccg tctcgataac
     7081 ccgctcaccg gtaaaggact gaagtttgct cagcggctgt gctacgtcaa cgccatgttc
     7141 cacttcttgt cgggcattcc acggctgatc ttcctgactg cgccgctggc gttcctgctg
     7201 cttcatgcct acatcatcta tgcgccagcg ttgatgatcg ccctattcgt gctgccgcat
     7261 atgatccatg ccagcctgac caactcgaag atccagggca aatatcgcca ctctttctgg
     7321 agtgaaatct acgaaacggt gctggcgtgg tatatcgcac caccgacgct ggtggcgctg
     7381 attaacccgc acaaaggcaa atttaacgtc accgccaaag gtggactggt ggaagaagag
     7441 tacgtcgact gggtgatctc gcggccctac atcttccttg ttctactcaa cctggtgggc
     7501 gttgcggtag gcatctggcg ctacttctat ggcccgccaa ccgagatgct caccgtggtc
     7561 gtcagtatgg tgtgggtgtt ctacaacctg attgttcttg gcggcgcagt tgcggtatcg
     7621 gtagaaagca aacaggtacg ccgatcgaac cgcgtggaga tgacgatgcc cgcggcaatt
     7681 gcccgcgaag atggtcacct cttctcgtgt accgttcagg atttctccga cggtggtttg
     7741 gggatcaaga tcaacggtca ggcgcagatt ctggaagggc agaaagtgaa tctgttgctt
     7801 aaacgcggtc agcaggaata cgtcttcccg acccaggtgg cgcgcgtgat gggtaatgaa
     7861 gttgggctga aattaatgcc gctcaccacc cagcaacata tcgattttgt gcagtgtacg
     7921 tttgcccgtg cggatacatg ggcgctctgg caggacagct acccggaaga taagccgctg
     7981 gaaagtctgc tggatattct gaagctcggc ttccgtggct accgccatct ggcggagttt
     8041 gcgccttctt cggtgaaggg catattccgt gtgctgactt ctctggtttc ctgggttgta
     8101 tcgtttattc cgcgccgccc ggagcggagc gaaacggcac aaccatcgga tcaggctttg
     8161 gctcaacaat gatgataacg cgatgaaaag aaaactattc tggatttgtg cagtggctat
     8221 ggggatgagt gcgttcccct ctttcatgac gcaggcgacg ccagcaacgc aaccactgat
     8281 caatgctgag ccagctgtag ccgcccagac ggaacaaaat ccgcaggtgg ggcaagtgat
     8341 gtcaggcgag cagggagctg atgcccccat cgtggcgcag aacggtcctt cgcgtgatgt
     8401 gaagctgacc tttgcgcaaa tcgcgccgcc gccgggcagc atggtgctac gtggtattaa
     8461 cccgaacggc agcattgagt ttggtatgcg cagcgatgaa gtggtgacga aggcgatgct
     8521 caacttcgaa tacacgccat cgccatcgtt actgcctgtc cagtcgcagt taaaggttta
     8581 tctcaatgat gagctgatgg gcgtgctgcc agtgaccaaa gaacagttgg gtaaaaaaac
     8641 gctggcgcaa atgcccatta atccactgtt tattaccgac ttcaaccgtg tgcggctgga
     8701 gtttgtcggc cattatcagg acgtgtgcga aaacccggcc agcaccacgc tttggctgga
     8761 tgttgggcgg agcagtggac tggatctgac ctatcagacc ctgaatgtga agaatgacct
     8821 gtcacacttc ccggtgccat tctttgaccc gcgcgataac cgtactaaca ccttgccgat
     8881 ggtctttgcg ggtacgccgg atgttgggct gcaacaagca tctgccattg tcgcctcgtg
     8941 gtttggttcg cgttctggct ggcgtgggca gaacttcccg gtgctctata accaattgcc
     9001 ggatcgcaat gccattgtct ttgcaaccaa cgacaaacgg ccggacttcc tgcgcgatca
     9061 tccggcggta aaagccccgg tgattgagat gattaaccat ccgcagaatc cttacgtcaa
     9121 actgctggtg gtgtttggtc gtgacgacaa agacctgttg caggcagcga aaggtatcgc
     9181 tcagggtaac attctgttcc gtggtgaaag cgtggtagtg aatgaagtga aaccgctgct
     9241 accgcgtaag ccgtacgatg cgccgaactg ggtacgtacc gatcgtccgg tcacatttgg
     9301 cgaactgaaa acctatgaag aacagttaca atccagcggc cttgagccag cagcgattaa
     9361 cgtttcgcta aacctgccgc cggatctcta cctgatgcgc agtaccggca ttgatatgga
     9421 tattaattac cgctacacca tgccgccggt gaaagacagt tcgcggatgg atatcagcct
     9481 gaataaccag ttcctgcaat ccttcaacct gagcagcaaa caggaggcga accgtctgct
     9541 gctgcgtatt ccggtattac aaggtttgct ggatggcaaa acagatgtct ctattccggc
     9601 gctgaaactg ggcgcgacca accagctgcg cttcgacttt gagtatatga acccgatgcc
     9661 aggcggttcg gtagataact gtattacctt ccagccggtg cagaatcatg tggtgattgg
     9721 tgacgattcc accatcgact tctcgaagta ttaccacttc atcccgatgc cggatctacg
     9781 cgcctttgct aacgcgggct tcccattcag ccggatggcg gatctgtcgc aaaccatcac
     9841 cgtgatgccg aaagcgccta acgaagcaca gatggaaacg ttgctgaata ctgttggttt
     9901 tatcggcgca cagacgggct tcccggcgat taatctgacg gtgaccgatg atggcagcac
     9961 cattcagggc aaagatgccg acatcatgat catcggtggt atcccggaca aactgaaaga
    10021 cgataagcag atcgacctat tggtgcaggc gaccgaaagc tgggtgaaaa caccgatgcg
    10081 ccagaccccg ttccccggca ttgtgccgga cgagagcgat cgcgcggcag aaacccagtc
    10141 aacgctgacc tcttccggtg cgatggcggc ggtgattggc ttccagtcgc cgtataacga
    10201 ccagcgcagc gtgattgcgc tgttggcaga tagcccacgc ggttatgaaa tgcttaacga
    10261 tgcggtgaac gatagcggca aacgcgccac catgttcggt tcggtcgcgg tgatccgcga
    10321 gtccggtatc aacagcctac gtgttggcga cgtttattac gtaggtcatc tgccgtggtt
    10381 cgagcgcttg tggtatgcgc tggcaaacca tccgattctg ctggcggtgc tggcgactat
    10441 cagtgtgata ttgctggcat gggtactgtg gcgtctgctg cgaattatta gtcgtcgtcg
    10501 tcttaacccg gataacgagt aattgaagat gaatgtgttg cgtagtggaa tcgtgacgat
    10561 gctgttgttg gctgccttta gtgttcaggc agcctgtacc tggcctgcct gggagcagtt
    10621 taaaaaggat tacatcagtc aggaagggcg cgtcatcgac cccagcgacg cgcgcaaaat
    10681 caccacctcc gaagggcaaa gttacggtat gttctttgcc ctggcggcta acgaccgtgc
    10741 agctttcgat aatattctcg actggacgca gaacaatctc gctcagggtt ctttaaaaga
    10801 acgtttgccc gcctggctgt ggggcaagaa agagaacagt aagtgggaag tgctggacag
    10861 caattcggcc tccgatggtg atgtctggat ggcctggtcg ttgctggagg cggggcgttt
    10921 gtggaaagag cagcgttata ccgacatcgg cagcgcgttg ctaaaacgta tcgcgcggga
    10981 ggaagtggtg acggtgcctg ggctgggttc catgttgtta ccgggcaaag tgggttttgc
    11041 tgaggataac agctggcgtt ttaaccccag ctacatgccg ccgacgctgg cgcagtattt
    11101 cacccgcttt ggcgcgccgt ggaccacgct gcgcgaaacc aatcaacatt tattgctgga
    11161 aaccgccccg aaaggctttt cgccagactg ggtgcgctat gagaaagaca aaggctggca
    11221 gctaaaagcc gaaaaaacat tgatcagcag ctacgacgct atccgcgttt acatgtgggt
    11281 aggcatgatg cctgacagcg atccgcaaaa agcgcggatg ctcaaccggt ttaaaccgat
    11341 ggcgacattc actgagaaaa acggttatcc gccggaaaaa gtggatgtgg ctacggggaa
    11401 agcgcagggt aaaggaccgg tcggtttttc tgccgccatg ctgccttttt tacaaaaccg
    11461 cgatgcgcag gcagttcagc gccagcgcgt ggccgataac tttcccggca gcgataccta
    11521 ttacaactat gtgctgaccc tgtttggaca aggctgggat caacaccgtt tccgcttctc
    11581 gacaaaaggt gagttattac ctgactgggg ccaggaatgc gcaaattcac actaaacata
    11641 ttcacgcttt ccctcggtct ggccgtcatg ccgatggtcg aggcagcacc aaccatcagc
    11701 agaccctgcg gatggacagc ggcaacacta atgccgtgcg cgggctggcg aatatttacc
    11761 gccagcagtc gccagaaaaa gctgaagcgt ttatcgcctc gctctctgcc agccagcggc
    11821 gcagcattga tgatatcgaa cgcagcctgc aaaacgaccg tctggcacag caggcagagg
    11881 tactggaaaa ccagggcaaa tgggcgcagg cggcagcact tcagcggcaa cgactggcgc
    11941 tgggccccgg cagcgtatgg attacttacc gactggcagg aagaagctgc gcggcaatcc
    12001 aacgatggcg gcagcagtca gggcttcggc tacacggcgc gggcattact tgaacgacgt
    12061 gttacttcca actggtttgt tggcacggca attgatatcc agcaggtgaa agattacgca
    12121 cccagccatt tcctgctcta cgtacgttat tccgccgccg gatggcaggg tgacatggat
    12181 ttaccgccgc agccgctgat accttacgcc gactggtaag ttttcagata gcgcctctct
    12241 taatgccgct gcgatcgggt atactcgggc ggcaatctgg gatttccggg gggagacaat
    12301 ttgcgcgtaa gtcgctcgtt aacaatcaag cagatggcaa tggtggcagc cgttgtcctg
    12361 gtgttcgttt ttattttttg caccgttttg ctgttccatc tggtccagca gaatcgctat
    12421 aacacggcta cgcaactgga aagcattgct cgctctgtcc gcgaaccctt atcttcagct
    12481 attttgaaag gcgatattcc cgaagcggaa gctattcttg ccagcattaa accggcaggc
    12541 gtggtcagcc gtgccgatgt agtgctgcct aaccagttcc aggcgctgcg taaaagtttt
    12601 attccagagc gtccggtgcc ggtaatggtt actcgcctgt ttgagctacc ggttcaaatc
    12661 tcgctgggcg tctactcgct cgaacgtccg gcaaatccgc agccaatagc ctatctggtg
    12721 ctacaggcgg attccttccg tatgtataag ttcgtgatga gcactctctc aacgttagtg
    12781 accatttact tacttttgtc gcttatcctg accgtcgcca tcagctggtg cattaaccgc
    12841 ctgattttgc atccgttacg caatattgct cgcgaactta acgccatccc agcccaggag
    12901 cttgttggtc accaactggc attaccgcgt ctgcatcagg acgatgaaat cggtatgttg
    12961 gtgcgcagtt acaacctcaa ccagcaattg ctgcagcgcc attatgaaga acagaacgaa
    13021 aatgcgatgc gcttcccggt gtcggatttg ccgaacaaag ccttgctgat ggagatgctg
    13081 gagcaggttg tcgcgcgtaa acaaaccacc gcgctgatga tcatcacctg tgaaaccctg
    13141 cgtgatactg cgggcgtgct gaaagaggcg caacgagaaa ttctgctgct gacgctggtg
    13201 gaaaaactca aatcggtact gtcgccacgt atgatcctcg cgcagattag cggttatgac
    13261 tttgctgtca ttgccaacgg tgtacaggaa ccgtggcacg caatcacttt gggtcagcaa
    13321 gtactcacta tcatgagcga gcgcctgccg attgaacgta ttcaactccg tccgcactgt
    13381 agcattggcg tggcgatgtt ctacggcgat ctcaccgccg aacagcttta cagtcgcgct
    13441 atttctgcgg catttaccgc tcgccataaa ggcaagaatc agattcagtt ctttgatccg
    13501 cagcagatgg aagcagccca gaagcggttg acggaagaga gcgatatcct taatgcactg
    13561 gaaaatcatc agtttgcaat ttggttacag ccacaggtcg agatgaccag cggtaaactg
    13621 gtcagtgcgg aagtgttact gcgtatccag caaccggatg gcagttggga cctgccggat
    13681 ggcttaatcg atcgcattga gtgctgtggg ctgatggtta ccgtcggtca ctgggtgctg
    13741 gaagagtcct gtcgattgct tgcagcctgg caagagcgcg gcattatgct gcccttgtcg
    13801 gtaaacctct ctgcgctgca actgatgcac ccgaatatgg tggcggatat gctggaactg
    13861 ttaacccgct atcgcattca gccgggaaca ctgattctgg aagtgacaga aagccgacgt
    13921 attgacgacc ctcatgctgc ggtggcaatc ctccgtccgc tgcgcaatgc cggagttcgg
    13981 gtggcgctgg atgatttcgg catgggctac gcagggctgc gtcagctgca gcatatgaaa
    14041 tcgttgccaa tcgacgtact gaaaatcgac aaaatgtttg ttgaaggctt gccggaagat
    14101 agcagcatga ttgctgcaat tatcatgctg gcgcagagcc tgaacttaca aatgattgcc
    14161 gaaggcgtgg agactgaagc acaacgagac tggctggcaa aagcgggcgt tggtattgcc
    14221 cagggcttcc tttttgctcg cccactccct attgaaatct tcgaagagag ttacctggaa
    14281 gaaaagtagc taccccaaac tgattacaaa actttaaaaa gtgctggttt gtgcgagcca
    14341 gctcaaactt tttaaccttt ttgtttcaat tatgatccag gtacatttct gtgatgttgt
    14401 ctgggtgtta ttttaaggcc gcaggtaccc ccccccccat aaccttacaa gacctgtggt
    14461 tttactaaag gacaccctat gaaaacctct ctgtttaaaa gcctttactt tcaggtcctg
    14521 acagcgatag ccattggtat tctccttggc catttctatc ctgaaatagg cgagcaaatg
    14581 aaaccgcttg gcgacggctt cgttaagctc attaagatga tcatcgctcc tgtcatcttt
    14641 tgtaccgtcg taacgggcat tgcgggcatg gaaagcatga aggcggtcgg tcgtaccggc
    14701 gcagtcgcac tgctttactt tgaaattgtc agtaccatcg cgctgattat tggtcttatc
    14761 atcgttaacg tcgtgcagcc tggtgccgga atgaacgtcg atccggcaac gcttgatgcg
    14821 aaagcggtag cggtttacgc cgatcaggcg aaagaccagg gcattgtcgc cttcattatg
    14881 gatgtcatcc cggcgagcgt cattggcgca tttgccagcg gtaacattct gcaggtgctg
    14941 ctgtttgccg tactgtttgg ttttgcgctc caccgtctgg gcagcaaagg ccaactgatt
    15001 tttaacgtga tcgaaagttt ctcgcaggtc atcttcggca tcatcaatat gatcatgcgt
    15061 ctggcaccta ttggtgcgct cggggcaatg gcgtttacca tcggtaaata cggcgtcggc
    15121 acactggtgc aactggggca gctgattatc tgtttctaca ttacctgtat cctgtttgtg
    15181 gtgctggtat tgggttcaat cgctaaagcg actggtttca gtatcttcaa atttatccgc
    15241 tacatccgtg aagaactgct gattgtactg gggacttcat cttccgagtc ggcgctgccg
    15301 cgtatgctcg acaagatgga gaaactcggc tgccgtaaat cggtggtggg gctggtcatc
    15361 ccgacaggct actcgtttaa ccttgatggc acatcgatat acctgacaat ggcggcggtg
    15421 tttatcgccc aggccactaa cagtcagatg gatatcgtcc accaaatcac gctgttaatc
    15481 gtgttgctgc tttcttctaa aggggcggca ggggtaacgg gtagtggctt tatcgtgctg
    15541 gcggcgacgc tctctgcggt gggccatttg ccggtagcgg gtctggcgct gatcctcggt
    15601 atcgaccgct ttatgtcaga agctcgtgcg ctaactaacc tggtcggtaa cggcgtagcg
    15661 accattgtcg ttgctaagtg ggtgaaagaa ctggaccaca aaaaactgga cgatgtgctg
    15721 aataatcgtg cgccggatgg caaaacgcac gaattatcct cttaatctca ccacttatgc
    15781 ccgcactccc ttccccgagt gcgggtgttt gcgcataatt gacccctgtg cctgaactta
    15841 ccacctgggt gagttcaggt ttatttcact tcttccgtga cattttcatg ttcttgcggt
    15901 ctaacacgaa gtgtttttaa cgtcatttag actgaccgac aacgggcagt ttttttatta
    15961 ccaggatagt tgatcagggg ttcacatgca gggcataaaa attcgacttt tagcgggcgg
    16021 tttgctgatg atggccactg ctggctatgt gcaggcagat gcgctccagc ctgatccagc
    16081 atggcaacag gggacgcttt ccaacggttt acagtggcaa gtgctgacca ccccccagcg
    16141 tcccagcgat cgtgttgaaa ttcgcctgct ggttaatacc ggttcgctcg ccgaaagtac
    16201 acaacagagc ggttacagtc acgccatccc tcgtattgcg ctaacgcaaa gcggtggcct
    16261 tgacgcagca caggcgcgtt cattgtggca gcaggggatc gaccctaaac gcccgatgcc
    16321 gccggtaatt gtctcttatg acaccacgct gtttaatctg agtttgccca ataaccgtaa
    16381 cgatttgctg aaagaagcgc tctcttatct ggcaaatgcc actggcaaat tgaccatcac
    16441 accagaaacc atcaaccacg cgctgcaaag tcaggacatg gtggcaacct ggcctgccga
    16501 tactaaagag ggctggtggc gctatcgtct gaaagggtca accttgttag gtcacgatcc
    16561 tgccgatccg ctgaaacaac ccgttgaagc ggaaaaaatt aaagatttct atcagaaatg
    16621 gtacaccccg gatgcaatga cgctactggt ggtgggaaac gtggatgcgc gctcggttgt
    16681 cgaccaaatc aacaaaacgt ttggcgaact gaaaggcaaa cgtgaaacgc cagctccggt
    16741 gccgacgctt tctccgctgc gtgcggaagc ggtgagtatt atgactgacg cggtgcgtca
    16801 ggaccggtta tctatcatgt gggatacgcc gtggcagccg attcgtgaat cagccgcact
    16861 gctgcgctac tggcgtgcgg acctggcccg cgaggcgctg ttctggcatg ttcagcaagc
    16921 gttaagcgcc agtgacagca aagacatcgg tcttggattt gactgccgtg tgctgtatct
    16981 gcgtgcgcag tgtgccatca acatcgaatc accaaacgac aagctgaaca gcaaccttaa
    17041 tctggtggcg cgtgaactgg cgaaggttcg cgataaaggt ctgccggaag aagagttcaa
    17101 tgcgttagtg gcgcaaaaga aactggagct gcagaaactg tttgccgcct atgcacgggc
    17161 tgataccgat attctgatgg gccagcggat gcgttcgttg caaaatcagg tcgtcgatat
    17221 cgcgccggag cagtatcaga aactgcggca ggatttcctt aacagcctga ctgtggagat
    17281 gttaaatcag gatctgcgtc agcagttgtc gaatgatatg gcgttaatac tgctgcagcc
    17341 gaaaggcgag ccggaattta acatgaaagc gttgcaggcg gcctgggatc aaatcatggc
    17401 cccatctacc gccgctgcga ccacctctgt cgccacggat gacgtacatc ctgaagtgac
    17461 ggatattcca cctgcacagt aatgcctgat ggtgatgagt tagcacaacc atcaggtatt
    17521 tatggatgag ctggatagag gggtaacact ttatccagcc ttttgcatat gctgcgttta
    17581 cgctggcatc gcctcacgcg ggataatcgc gccgcgatac tgaataacgg tacttgcggt
    17641 caggtgcccg cgtttcgccg cgttttccgc actgccgccc gtcaaacgta ccgccagata
    17701 accggcgctg aaagagtcgc cagctgcggt ggtatcgatc actttttctt tcggcagttt
    17761 caccgccgga acatcaacta acgcttcacc agcaatggac accaggcaag aatccgcccc
    17821 gcgtttcacc accacttctt tcacgcccgc gttatgggtg cgcgcaatga cgtcttccac
    17881 cggctgttga ccccacagcg cgtcttcgtc gtccagcgtc aggaaggcga tatccgtgca
    17941 ttccagcatt tgctggtaca cctgctgtgt ctcttctttg ctggcccaca ggcgcggacg
    18001 atagttattg tcgaaaatca cttttccgcc tttggcgcgg cattcgcgca gcagggaaag
    18061 cagcttttcg cggctggtcg ggcttaagat cgccaggcta atcccgctca ggtagagata
    18121 atcgaaattc gccagctctt cgcaaatcgc cgcagactgc tcgctcgcca gccagaattt
    18181 ggcggcggct tcgttacgcc agtagtagaa cgtacgttcg ccggtgctgt cggtttcaat
    18241 gtagtaaagg cccggaagac ggttttccat ccgttgggtc agggaagtat caacgttctc
    18301 gccgtgccag gcgtccagca tctgctggct aaaactgtcc gttcccagcg ccgttacgta
    18361 atgaacggtt aatgccgcag gatcgacctg acgggcgata tagacggaag tgttcagggt
    18421 atcgccgccg aaaccgcgct taacgtccgc gcctttctcg gaaagctcaa tcatgcattc
    18481 gccaatcacg gcaatctttt tggacatagt cgtgaacctg atctgtaaaa aattagcgtt
    18541 agtttgcgct gtggtggttt gctggtcaac catattaaaa cagtgttcca ttattttttt
    18601 gagccagaac gtattcctga aagattccgt tgtggagtga ggaaatttac cccggccagg
    18661 cggtaaagtt ctgcccttac gcgccgataa tctttgtcga gtccgggcag catcactttt
    18721 aaacacagga catctttgat gataaggcag gttatccagc gaataagcaa ccctgaagca
    18781 agcatcgaga gcttgcagga acggcgtttt tggttgcagt gtgagcgtgc ttacacctgg
    18841 cagccgatct atcaaacatg cgggcggtta atggccgtgg agctattaac ggtggtcacg
    18901 catcccttga acccttcgca acgcctgccg ccggatcgct attttactga aatcaccgtc
    18961 agccatcgga tggaggttgt gaaagagcag attgatttgc tggcgcaaaa agccgacttc
    19021 tttgtagagc acggcctgct ggcatcggtc aatattgatg gccctacgct catcgccctg
    19081 cgtcagcaac caaaaatcct gcgccagatt gagcgtcttc cctggctgcg tttcgaactg
    19141 gtggagcata tccgtctgcc gaaagattca acctttgcct cgatgtgtga atttggcccg
    19201 ctgtggctgg atgattttgg taccgggatg gcaaatttct ctgcactaag tgaagtgcgt
    19261 tatgactaca tcaaaatcgc gcgagaactg tttgtgatgc tgcgtcagtc gccggaaggc
    19321 cgcacactct tttctcagct tttacatcta atgaatcgct attgtcgcgg ggtgattgtc
    19381 gagggcgtag aaacaccgga agagtggcgt gatgttcaga actcgcccgc attcgccgca
    19441 caaggctggt ttctttcacg cccggcaccg atggaaacgc tgaatacggc ggttctggcg
    19501 ctataagctg cctcattttc cgccgggctg gactatcttt aggactggca caggaagaat
    19561 atgaggaagc gaacgatgag caaggcaggc aaaataaccg ctgcgatttc aggggctttc
    19621 ttgttgttga ttgtcgtggc gatcattttg attgcaactt ttgactggaa tcgactcaaa
    19681 ccgaccatca accagaaagt ctctgcggag ttgaatcgtc cgttcgctat ccgtggcaat
    19741 ctgggcgtgg tgtgggagcg gcaaaaacaa gaaactggct ggcgcagctg ggtgccgtgg
    19801 cctcatgtac atgcagaaga catcattctt ggtaatccac cggatattcc cgaagtcacg
    19861 atggtgcatt tgccgcgagt agaggcaacg ctggcaccgc tggcgctgct gaccaaaacg
    19921 gtctggctgc cgtggatcaa gctcgaaaag cccgacgcgc gcctgattcg cctctctgaa
    19981 aagaacaata actggacgtt taatcttgcc aacgatgaca acaaagacgc gaatgcaaag
    20041 ccgtcggcat ggtcgtttcg gctggataat attcttttcg atcaagggcg gatcgccatt
    20101 gatgacaaag taagcaaagc ggatctggaa atttttgttg atccgttagg caagccgctg
    20161 ccattcagcg aagttactgg atcgaaaggt aaagcggata aagaaaaggt gggcgattac
    20221 atttttggcc tgaaggcgca gggacgttat aacggtgaac cgctcacggg tacgggaaaa
    20281 ataggcggta tgctggcgct gcgtggcgaa gggacgccgt ttccggtaca ggctgatttc
    20341 cgttcaggta atacccgtgt tgcttttgat ggcgtcgtga atgacccaat gaagatgggc
    20401 ggtgtcgatt tacggcttaa attttctggc gattcactgg gtgatctcta tgaactgacg
    20461 ggcgttctgc tgcccgatac cccgccgttt gaaacggatg gtcggctggt agcgaaaatc
    20521 gacactgaaa aatcgtcggt ctttgattat cgcggtttta atgggcgaat tggcgatagc
    20581 gatatccacg gttctctggt ctacaccacc ggaaagccac gaccaaaact ggaaggtgat
    20641 gtcgagtcgc ggcaattgcg gctggcggac ctgggaccgt tgattggcgt tgattccggg
    20701 aaaggggcag aaaagtcgaa acggtctgaa cagaagaagg gcgaaaaaag cgttcagcct
    20761 gcgggcaaag tgctgcctta tgaccgcttc gaaaccgata aatgggacgt tatggatgcc
    20821 gatgttcgct tcaaagggcg gcgcattgag catggcagta gcctgccgat tagcgatctt
    20881 tctactcata tcatcctcaa aaatgctgac ctgcgcctgc aaccgctgaa atttggcatg
    20941 gcgggcggca gcattgcggc gaatattcat ctggaaggcg ataaaaagcc gatgcagggg
    21001 cgggcagata ttcaggctcg tcgactgaaa ctgaaagaac tgatgcccga tgtggaactg
    21061 atgcagaaga cgctggggga aatgaacggt gacgcggaac tacgcggtag cggtaactcg
    21121 gtggcggcgc ttttaggcaa cagtaacggc aacctgaaac tgttgatgaa tgacgggctg
    21181 gtgagccgca acctgatgga gattgttggg ctgaatgtcg gcaactacat tgtcggtgcg
    21241 atatttggtg acgatgaggt gcgggtgaac tgcgcggcgg cgaatctgaa tattgccaac
    21301 ggcgtggcgc gcccgcagat ttttgctttc gatactgaga acgcgttgat taatgttacc
    21361 ggcacggcaa gttttgcttc ggaacagctg gatttgacta ttgatccgga gagtaaaggg
    21421 attcggatta tcacactgcg ttcgccgctg tatgtgcgtg ggacgtttaa aaatccgcag
    21481 gctggggtga aagccggacc gctgattgcc cgtggtgctg ttgctgcggc actggcaacg
    21541 ctggtaacac cggcggcggc gttactggca ctgatctcac cttccgaagg ggaggctaat
    21601 cagtgtcgga cgatattgtc gcagatgaag aagtgatgat aattgccgga tgtctgacat
    21661 ccggcaatta aacagattta gatttttggc aaagtgtgct ttgttcatgc cggatgcggc
    21721 gtgaacgcct tatccggcct acaaatcctt gtgaattcaa tatattgtat ggaaattcag
    21781 tcctgataag cgtagcgcat caggcttttc acacttatta caacgactga tgtcgcgtct
    21841 catgggtcag cagcagggcg attaacgtca agccagccat cgccgccaga tataacccca
    21901 ccgcacctaa cccgtagtta gcctgcaacc aggctgcgat atacggcgca acggaagccc
    21961 cgagaatcga cgatacgttg taagagaacg atgctccggt gtaacgcact tctgtcggaa
    22021 acagctctgg taacagcgca cccatcggcc cgaaggtcag ccccatcaga cttaacccca
    22081 gcagcaggaa ggcaaaaacc agaatcgggt tgccagaacc gagcagcggg ttaaaggcga
    22141 acagcgcgaa caggatgatc agcgtggtga tgatcaccat gcttttacgg cgaccaaagg
    22201 catcagccag taatccggcg actggcacca tcacgccaaa accaataact gccatcatca
    22261 gcatccacaa cacttcgtta cgcggcaggc caagcccaac tggcgcggcg gcggtactga
    22321 aggtcataga gtagaccgtc atgatgtaaa acagcgtata ggttgccagc ataatgaacg
    22381 tacccagtac ggttacgcga acatgtttgg tcagcagcgt acccagcggg atcttcacct
    22441 gttttttcgc tttagcgact ttctcaaaca ccggcgactc atgcagcgac acgcgaacat
    22501 acaggccgat aatgaccagc accgccgaga agataaatgg cacacgccag ccccagctca
    22561 taaactgctc atcggtcagt agccaggaaa gcagcaagaa agtgccgttg gcaaagaaga
    22621 agccaatcgg tgcgcctagc tgtgggaagg agccatacag tgcacgtttg cgcggtgggg
    22681 cgttttcagt cgccagcagc gccgcgccgc cccattcacc gcctaagccc agaccctgac
    22741 caaatcgagc cagcgccagc agcagtgggg cgaaaatacc aatcgtggca tagcccggca
    22801 gcagaccaat caccacggtc gaaatcccca tcgttagcaa cgaggcgacc agcgtcgctt
    22861 tacgcccaac gcgatcgcca aaatgaccaa aaacggcaga gccaatgggg cgcgcgacga
    22921 aggcgatggc gaaggtggcg agcgactgta gcgttgctgc cgttggatcg ccctgcggga
    22981 agaagatatg cggaaacaca ataacggccg cagtggcgta aatgtaaaag tcgaagaact
    23041 caatggccgt gccaatgaga gaggcgacaa ggactttatt acgcgagttg atcggcgtgt
    23101 attcttgttc gtggtcgagt gttgtggctg ttgcttgcat aatcttttct tattttttgg
    23161 ctaacgaata gccatattac gcacagcaaa agtgatattt caat
//
LOCUS       DAZSIO010000050        23190 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZSIO010000050 DAZSIO010000000
VERSION     DAZSIO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.050"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..246
                     /locus_tag="ACHX5T_001808"
     CDS             <1..246
                     /locus_tag="ACHX5T_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR8467040.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(293..1051)
                     /gene="deoR"
                     /locus_tag="ACHX5T_001809"
     CDS             complement(293..1051)
                     /gene="deoR"
                     /locus_tag="ACHX5T_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR8467041.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="ACHX5T_001810"
     CDS             complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="ACHX5T_001810"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR8467042.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1990..3216
                     /gene="mdfA"
                     /locus_tag="ACHX5T_001811"
     CDS             1990..3216
                     /gene="mdfA"
                     /locus_tag="ACHX5T_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFR8467043.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3257..3541)
                     /gene="ybjH"
                     /locus_tag="ACHX5T_001812"
     CDS             complement(3257..3541)
                     /gene="ybjH"
                     /locus_tag="ACHX5T_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR8467044.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="ACHX5T_001813"
     CDS             complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="ACHX5T_001813"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR8467045.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4442..5650)
                     /locus_tag="ACHX5T_001814"
     CDS             complement(4442..5650)
                     /locus_tag="ACHX5T_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8467046.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5734..6270
                     /gene="rcdA"
                     /locus_tag="ACHX5T_001815"
     CDS             5734..6270
                     /gene="rcdA"
                     /locus_tag="ACHX5T_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR8467047.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6445..8130)
                     /locus_tag="ACHX5T_001816"
     CDS             complement(6445..8130)
                     /locus_tag="ACHX5T_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR8467048.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8400..8777
                     /locus_tag="ACHX5T_001817"
     CDS             8400..8777
                     /locus_tag="ACHX5T_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR8467049.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="ACHX5T_001818"
     CDS             complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="ACHX5T_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR8467050.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9224..9511
                     /locus_tag="ACHX5T_001819"
     CDS             9224..9511
                     /locus_tag="ACHX5T_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR8467051.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9495..10217
                     /gene="nfsA"
                     /locus_tag="ACHX5T_001820"
     CDS             9495..10217
                     /gene="nfsA"
                     /locus_tag="ACHX5T_001820"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR8467052.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10278..11180
                     /gene="rimK"
                     /locus_tag="ACHX5T_001821"
     CDS             10278..11180
                     /gene="rimK"
                     /locus_tag="ACHX5T_001821"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR8467053.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11268..11744
                     /locus_tag="ACHX5T_001822"
     CDS             11268..11744
                     /locus_tag="ACHX5T_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR8467054.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12096..13208
                     /gene="potF"
                     /locus_tag="ACHX5T_001823"
     CDS             12096..13208
                     /gene="potF"
                     /locus_tag="ACHX5T_001823"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR8467055.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13303..14469
                     /gene="potG"
                     /locus_tag="ACHX5T_001824"
     CDS             13303..14469
                     /gene="potG"
                     /locus_tag="ACHX5T_001824"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR8467056.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLR
                     PINMMFQSYALFPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQ
                     LSGGQRQRVALARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVT
                     HDQEEAMTMAGRIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQE
                     DGLVLDSPGLVHPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHI
                     AYLGDLSVYHVRLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14479..15432
                     /gene="potH"
                     /locus_tag="ACHX5T_001825"
     CDS             14479..15432
                     /gene="potH"
                     /locus_tag="ACHX5T_001825"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR8467057.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15429..16274
                     /gene="potI"
                     /locus_tag="ACHX5T_001826"
     CDS             15429..16274
                     /gene="potI"
                     /locus_tag="ACHX5T_001826"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR8467058.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSLKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16334..16822
                     /locus_tag="ACHX5T_001827"
     CDS             16334..16822
                     /locus_tag="ACHX5T_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR8467059.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16863..17990
                     /gene="rlmC"
                     /locus_tag="ACHX5T_001828"
     CDS             16863..17990
                     /gene="rlmC"
                     /locus_tag="ACHX5T_001828"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR8467060.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18165..18896)
                     /gene="artJ"
                     /locus_tag="ACHX5T_001829"
     CDS             complement(18165..18896)
                     /gene="artJ"
                     /locus_tag="ACHX5T_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR8467061.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19188..19856)
                     /gene="artM"
                     /locus_tag="ACHX5T_001830"
     CDS             complement(19188..19856)
                     /gene="artM"
                     /locus_tag="ACHX5T_001830"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR8467062.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19856..20572)
                     /gene="artQ"
                     /locus_tag="ACHX5T_001831"
     CDS             complement(19856..20572)
                     /gene="artQ"
                     /locus_tag="ACHX5T_001831"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR8467063.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20579..21310)
                     /gene="artI"
                     /locus_tag="ACHX5T_001832"
     CDS             complement(20579..21310)
                     /gene="artI"
                     /locus_tag="ACHX5T_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR8467064.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21328..22056)
                     /gene="artP"
                     /locus_tag="ACHX5T_001833"
     CDS             complement(21328..22056)
                     /gene="artP"
                     /locus_tag="ACHX5T_001833"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR8467065.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22274..22789)
                     /locus_tag="ACHX5T_001834"
     CDS             complement(22274..22789)
                     /locus_tag="ACHX5T_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR8467066.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT         5683 a         5738 c         6002 g         5767 t
ORIGIN      
        1 gtgaccatac cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg
       61 cagcttaccg caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac
      121 ggtaaatcca ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc
      181 tttggtagga tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc
      241 tcttaatctt ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca
      301 tcaacttaat acggtgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcac
      361 tcaccacaat atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt
      421 tactgtggtc gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg
      481 gcaactcttc aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcggaat
      541 aaaaagcgat atccgggcaa aaattattca acgtttgctg aaaatcgatg ggtttgaaaa
      601 tggcgttgct ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttctctt
      661 tcagcgccag aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa
      721 tcgcttcaat aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt
      781 ctaccagtgt cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt
      841 gatcgcttaa caggtaatgg ctggcactgc gcggttccag aacaatatag ccgccgagca
      901 aaacgacggg cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa
      961 gcagggcggc ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct
     1021 gcccgatacg ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc
     1081 cgctcaatac tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta
     1141 atgcaaaaca gacgcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt
     1201 gccagataat ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat
     1261 ccagcggcca gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca
     1321 ttaaaagtga gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa
     1381 tcaccgtacc gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc
     1441 cgatattttc gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa
     1501 acaggctgac cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc
     1561 cccacaacca aagtaccgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa
     1621 aaatcgccaa cgagatcatc cacggagccg agtctggcgt cgcgttaata agagagaata
     1681 gagagagatt caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa
     1741 tacaggcaag tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat
     1801 ttccctcaag cggcctgttt acggtgggtg attgtaacgg gcataggtta aataaaactt
     1861 aaagaaagcg tagctatact cgcaataatg taagaatgtg cttaaccgtg gtttcagcta
     1921 caaaattcgc tttctcgtta gctgcgcttt tattaaactc tgcgcgatta ttattggcga
     1981 agaaattgca tgcaaaataa attagcttct ggtgccaggc ttggacgtca ggcgttactt
     2041 ttccctctct gtctggtgct ttacgaattt tcaacctata tcggcaacga tatgatccaa
     2101 cccggtatgt tggccgtggt ggaacaatat caggcgggca ttgattgggt tcctacttcg
     2161 atgaccgcgt atctggcggg cgggatgttt ttacaatggc tcctggggcc gctgtcggat
     2221 cgtattggtc gccgtccggt gatgctggcg ggtgtggtgt ggtttatcgt cacctgtctg
     2281 gcaatattac tggcgcaaaa cattgaacaa ttcaccctgt tgcgcttctt gcagggcata
     2341 agcttctgtt tcattggcgc tgtgggatac gccgcaattc aggaatcctt cgaagaggcg
     2401 gtttgtatca agatcaccgc gctgatggcg aacgtggcgc tgattgctcc gctacttggt
     2461 ccgctggtgg gcgcggcgtg gatccatgtg ctgccttggg aggggatgtt tgttttgttt
     2521 gccgcattgg cagcgatctc ctttttcggt ctgcaacgag ctatgcctga aaccgccatg
     2581 cgtataggcg agaaactgtc actgaaagaa ctcggtcgtg actataagct ggtgctgaag
     2641 aacggccgct ttgtggcggg ggcgctggga ttcgttagcc tgccattgct ggcgtggatc
     2701 gcccagtcgc cgattatcat cattaccggg gagcagttga gcagctatga atatggcttg
     2761 ctgcaagtgc ctattttcgg ggcgttaatt gcgggtaact tgctgttagc gcgtctgacc
     2821 tcgcgccgca ccgtacgttc gctgattatt atgggcggct ggccgattat gattggtctg
     2881 ttggtcgctg ctgcggcaac ggttatctca tcgcacgcgt atttatggat gaccgccggg
     2941 ttaagtattt atgctttcgg tattggtctg gcgaatgcgg gactggtgcg attaaccctg
     3001 tttgccagcg atatgagtaa aggtacggtt tctgcggcga tgggaatgct gcaaatgctg
     3061 atctttaccg tcggtattga aatcagcaaa catgcctggc tgaacggggg caacggactg
     3121 tttaatctct tcaaccttgt caacggaatt ttgtggctgt cgctgatggt tatcttttta
     3181 aaagataaac agatgggaaa ttctcacgaa gggtaaaaaa atgcctgatt gatttgtgcg
     3241 atcaggcatt ctcgaattaa tggtgatggt cgtcaatctg gtgttcgata accatccctt
     3301 cacctacgct ggcaagatgg cgaacataag gatgcgggcg gtaagccgga gctggcgcag
     3361 gagccacata gacggtttgc ggcacagtcg cgacgctgac cgcttgtgga acgctgactg
     3421 aatcaggaac cacaaccact ttgtatccac tcggcacatc gacggtgaca ctttgcgcca
     3481 tcgccacacc agtaaagccc attaaaagcg cgcccaacag tagacaattt ttcataatca
     3541 tttcgcctga aataatgcag cgggttaatt tccgcgaatt atgcagcctt cattcaggca
     3601 ttgtgttgcc gggcgtgctg ccgtttttat tggtcaaatg gcgcttcgtg tttaagaact
     3661 ttatcgatca catccagtac gccttcacgg ttattgtagc ctgcccggta ttttgccgct
     3721 gcgacgactg cgctgccggc attttccatt gcaaaactaa agcctgcctg acgcagcatc
     3781 tcaatatcgt taccgccatc gccaaagacc accacttcgc tgtcgtctat tccccataat
     3841 ttctgcagtt ggcgaaggcc attggcttta tgtacgccgg ggataatcag atcgatgctg
     3901 ccgttgccgg tgtggactgg caccataata tcgccgatgg cctcatgtaa tgctttttgt
     3961 acttgtggaa tcagttcatc ggaaagattc aggccaaact taaagaaaat atcttctaag
     4021 ttgtcaaagt tatcgacgta ttccagacgg tgataataca tttccgccac cgttttcatg
     4081 gcatcgtcat attttttgag tgtataggcg ctattttttc cgcaggcaat aatttccact
     4141 tccgggcgcg tcagcaaatg ttccacgaca gtagcaaacg catccttcga cagctcgcca
     4201 ttaaaaacat ctttgccttc actcattacc cagccgccgt tttcagccac aaaggcaatt
     4261 tcattcgcaa tttcagggaa gaaagagatc aactgataat attgattccc gctggcgacc
     4321 acaaagcgaa tgccttgtgc tttcatttgc tgatactgag ccataaaccg ctcacggtta
     4381 taggtttttt gatcgcttaa gaaagtacca tccatgtcta ccgcaattaa tttaatgctc
     4441 atcaactatt ctccatcgcc gtctgcgttt tggtatcggg tttggcgacg gctttcgcga
     4501 caatagccgc gagaataacc agcgccagta caaccagcat tgcactacgt aatccataat
     4561 gttcgccgag atagcccagc agcggcggcc cgacgaggaa agccagataa ccggtcgttg
     4621 ctaccacact gacgcgggtt ggtgcatcgg ggccggtatc gctggcggca gaaatggtca
     4681 gcgggaagcc cagcgaggca cccagtcccc aaagtacaac agacactcct gcgacccagg
     4741 cgctatcgac aaaaataatc agcccaatac ccaacgcccc cattagtgca ctggcccgaa
     4801 ccacggcaac gcgactgtaa ctgtcgatga accaaccgcc ggtgaagcgt ccaacggtca
     4861 tccctagggt aaaaccggca taaatcagtg agccggaagt ggggctaaaa ccgtgaccat
     4921 caaccattaa taagggtaac cagtcgttgg cagaaccttc ggcaaaggcc atcgccagca
     4981 ctacaacacc aatcaacagc aactggatat cgcgataaaa aggaatgcct ttttcgccat
     5041 gctgggtgcc atcggcagca tttttgcccg taccgtcagg gattgcctga atggcgatat
     5101 aaataggcgc gatacctacc agcgccgcca ataaaatgtg caccgttgcc ggaacgccaa
     5161 aggccgtcag tgccatcccg acaccagcgc ctgccagcgt gcccaggcta taaaaaccgt
     5221 gcatcatcgg caaaaccgtt ttattcattt ctcgctcaac ggcggcacct tcaacgttta
     5281 tcgccacttc cgcagaacca aaacttgccc caaagacgcc gagaccaacg gcaaagagca
     5341 ggggcgatgt cagccagagt gccagactta atatcatcat cccgatcaat gcgcaggaca
     5401 tcgtgaccag gatgacatta cgtgtcccaa agcgtttcac taaccacgcc gagcagagaa
     5461 taccgctcat cgaaccgatc gacagaccaa agagaacacc gcccatttca gcgatcgaga
     5521 cagagagaat atcgcggata gcaggcgtac gggttgccca ggacgccatt aacaggcctg
     5581 gcaaaaagaa gaacataaac agcgcccagg ttcggcgttt caatgcatta cgtgaagaat
     5641 ttacggtcat agatcacgtc aaaataagaa gagggaagac aacattagca aggttgtgta
     5701 catttgtaca caattgcaga cagaggaaat gacatgcgtc gcgctaacga tccgcaacgg
     5761 cgagaaaaaa ttatccaggc cacgctggag gcggtgaaac tttacggaat acatgctgtt
     5821 acgcaccgca aaattgctac ccttgccggg gtaccgttgg gatcgatgac ctactatttt
     5881 tcaggaattg atgagttgtt gctggaggcg ttcagcagtt ttactgagat tatgtcccgg
     5941 caatatcagg cattttttag cgatgttagt gatgctcaag gcgcatgcca ggctatcacc
     6001 gatatgatct acagctcaca ggttgcaacg ccggataata tggagctgat gtaccagctc
     6061 tacgcgctgg ctagccgaaa accgctatta aaaacggtaa tgcaaaactg gatgcagcgc
     6121 agtcagcaaa cgctcgaaca atggtttgaa cccggaaccg cccgcgcgct tgatgcgttt
     6181 attgagggga tgacgctgca ttttgtcacc gaccgtaagc cgctatcgcg cgaggagatt
     6241 ttgaggatgg ttgagagggt tgcagggtag tagataaatt ttagataaca aaaaacccat
     6301 caaccttgaa ccgaaatggc ggggttgatg ggctccacaa aatggggaca tcaaagaaaa
     6361 gcagtggcac taattaagac tgatgccctg cggaaaagtt ctgcggttgt gcaaaaaaat
     6421 ttcattttca gggcaacttc agttttatcc taatcctggc cataccatga cgatgattgt
     6481 ccctgccagc gtcagcagga cgttggcgat tgcgtaggtg cccgcatagc ccagcgcagg
     6541 gatgttactg cgagctgtat cactgatgat ctccattgcc ggcgcgcagg tgcgtgcgcc
     6601 catcattgcg ccgaacaaca gtgcgcggtt cattcgcaat acataagcac cgaacaagaa
     6661 acagataacc acgggcacaa gactgacaat taatccggca atcaacatct gaccgccaat
     6721 cgcgcccagg ccgttattaa taccgctacc ggcgctcaga ccaacgcctg ccataaacac
     6781 catcaagccg aactctttca ccatgcttaa tgcaccttgc ggaatgtaac cgaaggtcgg
     6841 gtggttagca cgcataaagc ccagcataat tccggcgaat aacaacccgg cagcgttccc
     6901 catgccgaaa ctgaatgtgc tgaactggaa ggtgatcatc ccgatcatca gcccaataac
     6961 aaagaaggcg caaaatgcca gcaggtcagt gacctggctg tgaatcgaga taaagccgat
     7021 gcgatcggcg atggttttta cgcgacgggc atcaccgctg acttgtaaaa cgtcaccttt
     7081 gttaagcacg acgttgtcat ctatcggcat ctcaatctgg ctacgaatga cgcggttaag
     7141 gaagcaaccg tgatcggtca acttcagttg tgcgagacgt ttacctacag cgttatggtt
     7201 tttaacgacc acttcttcag tgacgatacg catgtcgaga aggtcacgat cgaaaacttc
     7261 tttaccgtta cggaagctgg gatcgagtcg ggcatgggcg tcgggatagc ctaccaacgc
     7321 tatttcatcg cccatttgta gcacggcatc accgtctgga tttgccagaa tcccgttacg
     7381 tcgaatacgt tcaatgtagc agccggtttg tcgataaata cccagttcac gcagattttt
     7441 gccgtcggtc caggccacca gctccgggcc gacgcgatag gcgcggatca ccggtaaata
     7501 aaccttacgg ttggcatcag tgtccaggcc acgttcgcgg gcgatttgct gggcgctggt
     7561 ctgtaagtcc tgatgctgca atttcggcaa gtaacgcgca ccaacaatca aactcaccag
     7621 accgattaaa taggttaagg catacccgag gctcagatta tccagtgcca gtgagagctg
     7681 cctgctttcc atgccggaat gatgcagtgt atcgccagca ccgaccagaa ccggtgtcga
     7741 cgtcatagag cctgctaaca taccggccgt caggccaata tcccagccaa acagcttacc
     7801 taaccctaag gcgatcacca gcgcactgcc aaccatcacc agtgctaaca ttaggtaatt
     7861 tttcccatcg cgaaaaaaaa tggaaaaaaa gttcggtccg gcttcgaccc cgacgcagaa
     7921 aataaacagc ataaagccaa gattaagcgc atcggtgtta atgctgaaat gttgttggcc
     7981 taataacagc gatacgacta aaacgccaat ggaattaccc agttggatcg aaccaagtcg
     8041 taactttccg agacatagcc caagcgcgag gaccacaaat aataacagaa tgtaattccc
     8101 atttaacaat tcggcgacgt ttatattcac ggaggctaac ttcttgttta ctagtaagct
     8161 gttgaaagaa atgctaattt acgataatgt gttttaccag aattcagggc gcagattcat
     8221 tcagcgcacc taaacgatag taaagtaaca atatatttta ctagtgtaat cacattaggt
     8281 atcaacggct atatgaattg cgttggccta tattagcatg gaatgcgaag cggctttatc
     8341 ttactgaacg ccacactggc gaaaaatgtg ttcgatagac gcagtgtcag gaggaacgag
     8401 tgaaacataa acaacgttgg gcgggggcaa tctgctgttt tgtcctcttc attgtggtgt
     8461 gcctttttct ggcgacgcac atgaaaggcg cttttcgggc tgccgggcat cctgaaatcg
     8521 gcttgctgtt tttcattctt cctggagcag ttgccagttt cttttcacag cgtagagaag
     8581 tcctgaaacc tctatttggc gcaatgctgg cggcaccctg ttcaatgctc attatgcggc
     8641 tgtttttttc accgacgcgc tcattctggc aagagctggc atggttacta agcgcggtgt
     8701 tctggtgtgc gctgggggca ctgtgtttct tatttatcag tagtttgttt aaaccacagc
     8761 acagaaaaaa tcagtaaagc cctcaacgcg agggcttgtc agacgatcag gcgtccagat
     8821 tttctttcac ccatgcagca aaatcggtat agccgccgat atgttgctga tcgacaaaaa
     8881 tctgcggcac ggtttctacg ggtttacctg ccttttgttg tagatcttct ttagtgatcc
     8941 cttccgcacg aatatctaca tactgatact gaaaatcatc gcgttcattg ctcaatttct
     9001 cagccagatc ttttgcacgc acacagtaag ggcaacccga acgaccaaaa ataacggttt
     9061 gcattatttc tctcctcata gatttatgcc tgtaatgatc acgctaaaat gtattcgctg
     9121 aaagtaggtt taacctgttg cattaattgc taaaagctat aactgttaaa cacaatacag
     9181 tgaaaagttt tagactgaag gctcactttg cagagggaag cgtatgcgcg cgatcggtaa
     9241 attgcctaaa ggcgtgttga tactggaatt tatcggaatg atgctactgg cggtggcgct
     9301 gctgtcggta agcgactccc tgtcgctgcc tgagccattt tctcggccag aagtgcagat
     9361 tctgatgatt tttctcggtg ttttgctcat gcttcccgct gcggtggtgg ttattcttca
     9421 ggtggcaaaa cgtcttgccc cacagctgat gaactgtcca ccgcaatatt cacgttcaga
     9481 aagagaaaaa gataatgacg ccaaccattg aacttatttg tggccatcgc gccattcgcc
     9541 atttcactga tgaacccatt tccgaagcgc agtgtgaggc gattattaac agcgcccgtg
     9601 cgacgtccag ttccagtttt ttgcagtgta gtagcattat tcgcattacc gacaaagcat
     9661 tacgtgaaga actggtgacg ctgaccggcg ggcaaaaaca cgtagcgcaa gcggcggagt
     9721 tctgggtgtt ctgtgccgac tttaaccgcc atttacagat ctgtccggat gctcagctcg
     9781 gcctggcgga acaactgttg ctcggtgtcg ttgatacggc aatgatggcg cagaatgcat
     9841 taaccgcagc ggaatcgctg ggattgggcg gggtatatat cggcggcctg cgcaataata
     9901 ttgaagcggt gacgaaactg ctgaaattac cgcagcatgt tctgccgctg tttgggctgt
     9961 gccttggctg gcctgcggat aatccggatc tcaagccgcg tttaccggcc tccattttgg
    10021 tgcatgaaaa cagctatcaa ccgctggata aagacgcact ggcgcagtat gacgagcaac
    10081 tggcggaata ttacctcacc cgtggcagca ataatcgctg ggatacctgg agcgatcata
    10141 tccgccgaac aatcattaaa gaaagccgcc catttattct ggattatttg cacaaacagg
    10201 gttgggcgac gcgctaaaac cgccacgtcg atgtatgata cgcgggcttt tgaccaggtc
    10261 tgacagagag gtgcagggtg aaaattgcca tattgtcccg ggatggaacg ctctattcgt
    10321 gtaagcggct gcgtgaagcc gctatacagc gcggtcacct ggttgaaatt cttgatccgc
    10381 tttcttgcta catgaacata aatcctgcgg cgtcttctat ttactacaaa ggccgcaagt
    10441 taccccattt tgacgcagtg atcccgcgta ttggcaccgc cattaccttt tatgggacgg
    10501 cggcactgcg ccagttcgag atgctgggga gctatccgct caatgagtcg gtcgccattg
    10561 cccgggcgcg tgacaaattg cgttccatgc aactgctggc gcgtcagggc atcgacctgc
    10621 ctgtcacggg cattgcgcat tcgccggatg ataccagcga tttaatcgac atggtcggtg
    10681 gtgcgccgct ggtggtcaag ttggttgaag gcacgcaggg aattggcgtc gtgctggcgg
    10741 agactcgtca ggcggcggaa agcgtgattg acgctttccg cggtctgaac gcgcatattc
    10801 tggtgcagga atatatcaaa gaggcgcaag ggtgcgatat ccgctgtctg gttgttggtg
    10861 atgaagtggt cgctgcgatt gaacggcggg cgaaagaggg ctattttcgt tccaatttgc
    10921 atcgtggcgg cgcggcaagc gtcgccagta tcacaccaca ggagcgtgaa atcgcgataa
    10981 aagccgcgcg aacgatggcg ctggacgttg ctggtgtgga tattctgcgt gctaatcgcg
    11041 ggccgttggt gatggaagtg aatgcgtcgc cggggctgga aggaatagaa aaaaccaccg
    11101 gtatcgatat cgcgggtaaa atgatccgct ggatcgaacg ccacgctacg acagaatatt
    11161 gcctgaaaac gggtggttag tcgcaatcac attactgatc atggttttgc ctgcgctttt
    11221 tgcgtaagct gtgccggtct ttttatcgaa agaggttgta caaaattatg acatcgctgg
    11281 tcgttcctgg tctggatacg ctgcgtcaat ggctcgatga cctggggatg agtttttttg
    11341 aatgtgataa ctgtcaggct ctgcatctgc cccatatgca gaatttcgac ggtgtctttg
    11401 atgccaaaat cgatctgatc gataacacga tcctgttttc tgccatggcg gaagtccgac
    11461 cttcagccgt attgccgctg gcggcggatt tatctgccat caatgccagt tcgctgaccg
    11521 tgaaagcatt tcttgatatg caggatgata atctgccaaa gctggtggtt tgccagtctt
    11581 tatccgttat gcagggcgta acctatgagc agtttgcatg gttcgtgcgt cagagcgaag
    11641 agcagatttc gatggtcatt cttgaagcta atgcccatca actgctgtta ccgactgacg
    11701 atgaagggca aaacaacgtt accgaaaact attttctcca ctgataattc ctttcgagca
    11761 cgcagtcgct gccgcagtga ctgcgcgctg caaaattatc tgctgttttt aaccttttct
    11821 taaagattat ttcacttctc ttgtgtcgat ttggctttat cacatagagc aaatacgcat
    11881 aaaaatttgt taaataccgt tttttaatcc gagctatagt ctcaaaccct ggctaaagtt
    11941 attcttgcga tgcttctata tagcgagcag tgctggccgg gagagagttc tcttttctta
    12001 caccgcgccg ataaaaaaat atgcacgttt attgcatatc tttcagtgtg acaacttttg
    12061 ttcgtttgtt aacgaacttt cagaaggaaa gagatgtgac cgccttaaat aaaaaatggc
    12121 tatcgggtct ggttgcgggt gctctgatgg ccgtctctgt cggcacgctc gcggctgaac
    12181 aaaaaacact ccacatttat aactggtctg attatatcgc ccctgacacg gtggccaatt
    12241 ttgaaaaaga aaccggtatt aaagtcgtct acgatgtttt cgactctaac gaagtactgg
    12301 aaggcaaatt aatggcgggt agcaccggct ttgacttggt ggttccatct gccagctttc
    12361 tagaacgcca gttgactgcg ggagttttcc agccgctgga caaaagcaaa ttgccggagt
    12421 ggaagaatct cgacccggag ctgctgaagc tggtcgccaa acacgatccc gacaataaat
    12481 ttgctatgcc ctatatgtgg gcgacgaccg ggattggcta taacgttgat aaagttaaag
    12541 ctgtgctggg cgaaaacgcg cccgtcgata gctgggactt gatcctcaaa cctgaaaatc
    12601 tggaaaaact gaaaagctgc ggtgtctctt tcctggatgc gccagaagaa gtttttgcta
    12661 ccgtgttgaa ttatctcggc aaagatccca acagcactaa agcggatgat tacaccggac
    12721 cggcaaccga tctgctgtta aagctgcgcc cgaacattcg ttatttccat tcatctcaat
    12781 acattaacga cctggcaaac ggcgatattt gcgtcgctat cggctgggca ggtgatgtct
    12841 ggcaggcgtc aaaccgcgcg aaggaagcga agaatggagt gaatgtctcg ttctcgattc
    12901 caaaagaagg ggcgatggcg ttctttgatg tattcgccat gccagcggat gccaaaaaca
    12961 aagacgaagc ctatcagttc ctgaattacc tgctgcgccc ggatgtagta gcgcatattt
    13021 ccgaccatgt gttctatgtc aacgccaata aagcagccac gccgctggtg agtgcggaag
    13081 tccgtgagaa cccgggtatt tatccgcctg cggatgttcg tgcgaagctg ttcactctga
    13141 aagtgcagga cccgaaaatc gaccgtgtgc gcacccgcgc gtggaccaaa gtgaagagcg
    13201 gaaaataatc cgcagcctta gatgccgggc gggcgcacca cacccgccgg caattcgcac
    13261 catcatggtg cgcttgcaca cattcaatgc cggagagcag ccgtgaatga cgctatccct
    13321 cgcccgcagg cgaaaacccg taaggcgctg acgccgctat tagaaatccg caacctcacc
    13381 aaatcctacg atggtcaaca tgcggtggat gatgtcagcc tgaccatcta caaaggtgaa
    13441 atcttcgcgc tgctgggcgc atccggctgt ggcaagtcca cgctgctggg cgcatccggc
    13501 tgtggcaagt ccacgctgct gcgtatgctg gcaggtttcg aacaaccttc tgccggacag
    13561 ataatgcttg atggcgtcga tttgtcacag gttccgcctt acctgcgccc catcaatatg
    13621 atgtttcagt cttacgcgct gtttccgcat atgaccgtgg aacagaacat cgcttttggc
    13681 ctgaaacagg acaaactacc gaaagcggaa atttccagtc gggtcaatga gatgctcggc
    13741 ctggtgcaca tgcaggagtt cgccaaacgt aaaccgcatc agctttctgg tggtcagcga
    13801 cagcgtgtgg ccctggcccg aagccttgcc aagcgcccga aactattact gctcgacgag
    13861 ccgatgggcg cgctggataa aaagctgcgt gaccggatgc agcttgaagt ggtggatatt
    13921 ctggagcgcg tcggtgtgac ttgtgtgatg gtcacccacg atcaggaaga ggcgatgacc
    13981 atggcggggc gcatcgccat tatgaatcgt gggaaatttg tccagattgg cgaaccggaa
    14041 gagatctacg agcatccgac tacccgctat agcgccgaat ttattggctc ggtaaacgtc
    14101 tttgaaggcg tactcaaaga gcgtcaggaa gatggcttgg tgcttgattc gccggggctg
    14161 gtgcatccac tgaaagtcga cgcggatgcc tcggtggtcg ataacgtgcc ggtacatgtg
    14221 gcgctgcgcc cggaaaaaat catgctttgc gaagagccgc ccgccaatgg ttgtaacttc
    14281 gcggtggggg aggtgataca cattgcctat ctcggcgatc tttcggtgta tcacgttcgt
    14341 ctgaaaagtg ggcagatgat tagtgcccag ctacaaaacg cccatcgtca tcgtaaaggg
    14401 ttaccgacct ggggcgacga agtgcgtttg tgctgggaag tggacagctg tgtggtgctg
    14461 acggtttaag gagcaaagat gaatacactt gaacctgctg cccagtcgaa accgccgggc
    14521 ggatttaagc tgtggttgtc gcagctgcaa atgaagcatg ggcgcaaact ggtcattgcg
    14581 ttgccatata tctggttgat cttgctgttt ctgctgccat ttctgattgt ctttaaaata
    14641 agcctggcgg agatggcgcg cgctattcca ccttataccg aactgatgga gtgggctgac
    14701 gggcaactgt ccatcactct taatctcggt aattttctgc aactgaccga cgatccgctc
    14761 tatttcgatg cttatctcca gtcgctacag gtggctgcga tttcgacatt ttgctgttta
    14821 ctgatcggct atccgctggc gtgggcggtg gcgcacagta agccttcgac ccgtaatatt
    14881 ttattactac tggtgatcct gccgtcgtgg acctcgtttc tgatccgcgt ttatgcttgg
    14941 atgggaatat taaaaaacaa cggtgtgctg aataattttc tgctgtggct gggggttatc
    15001 gatcaaccgc tgaccattct tcataccaat ctggcagttt atattggcat tgtttacgcc
    15061 tacgtgccgt ttatggtgct gccgatttat accgcgttga ttcgtattga ttattcgctg
    15121 gtggaagcag cgctggatct cggtgcacga ccgctgaaaa cgttctttac tgtgatcgtg
    15181 ccgctgacta aaggtgggat tattgccgga tcgatgctgg tgtttatccc ggctgtgggc
    15241 gagtttgtga tcccggaact gctcggtggc ccggacagca tcatgatcgg gcgcgtgcta
    15301 tggcaagagt ttttcaataa ccgcgactgg ccggtggcct cggcggtagc gatcatcatg
    15361 ttgctgctgc taattgtgcc gataatgtgg ttccacaaac accagcaaaa aagcgtggga
    15421 gaacacggat gaataattta ccggtagttc gttcgccctg gcggattgtg attttgctgc
    15481 tgggctttac ctttctctac gcgccaatgc tgatgctggt tatctattcg tttaacagct
    15541 tgaagctggt gacggtgtgg ggcggctggt caacgcgctg gtatggtgag ttattgcgcg
    15601 atgacgcgat gatgagtgcg gttggttcaa gcctgacaat tgctgcctgt gcggcaacgg
    15661 cggcggcgat cctcgggact attgcggcgg tggtgctggt gcgctttggc aggtttcgcg
    15721 gatcaaatgg ctttgccttt atgatcaccg cgccgctagt aatgccagat gtcatcacgg
    15781 gcttgtcgct gttgttatta ttcgtcgcgc ttgctcatgc cattggctgg cctgcggacc
    15841 gcggtatgct taccatctgg ctggcgcatg tcactttctg tacggcttat gtggcggtcg
    15901 ttatttcgtc gcgtctgcgg gaactggatc gctcgataga agaagccgcg atggatctcg
    15961 gtgcgacgcc gctgaaagtg tttttcgtca ttacgctacc gatgatcatg cccgcgatca
    16021 tttctggctg gttactggct tttactttgt cgcttgatga tctggtgatc gccagctttg
    16081 tttccgggcc gggagccacc acgttaccga tgctggtctt ttccagcgtg cggatggggg
    16141 tgaatccgga aatcaacgcc ctggcaacat tgatcctcgg cgcggtcgga attgtcggat
    16201 ttatcgcctg gtatctgatg gcgcgcgcag aaaaacagcg gatacgcgac atccaacgtg
    16261 caagacgtgg ctgaagacac taaaatttgc caacctggct acataatgcc gcgcatgtcg
    16321 cggcattgtt ttcatggaag acgaaacgtt gggatttttt aagaaaacat cttcatctca
    16381 tgctcgcctg aatgtgcctg cgctggtgca ggtggcggcg ctcgccatta ttatgatccg
    16441 tggcctcgac gtgctgatga ttttcaatac gctgggcgtg cgcggtattg gcgagttcat
    16501 tcatcgcagc gtacaaacct ggagtttaac gctggtcttt ttaagcagtc tggtgctggt
    16561 tttcattgag atctggtgtg cgttttcact ggtgaaaggg cgtcgctggg cgcgctggct
    16621 atatctgctg acacaaatca ccgccgcaag ttacttgtgg gcggcttcgc tggggtacgg
    16681 ttatccggag ctgttcagca ttcccggtga atcaaaacgt gaaatcttcc atagcctgat
    16741 gctgcagaag ctgccggata tgctcatcct gatgctgctg ttcgttccct cgaccagtcg
    16801 gcggttcttc cagttgcaat aatgtgtata atcgtcgccc ctgatgatgt gaaggtcaat
    16861 gtatgcagtg cgcactttac gacgcgggtc gctgtcgttc ctgtcagtgg ataacgcagc
    16921 cgattccaga gcaactctcc gctaaaaccg ccgatcttaa aaatctgctc gccgattttc
    16981 cggttgagga atggtgcgcg ccggtgtcag gcccggaaca agggtttcgt aataaagcca
    17041 aaatggtggt gagtggtagc gttgaaaaaa cactgctcgg tatgctgcat cgcgatggca
    17101 cgccggaaga cctttgtgac tgcccgcttt atccagcctc atttgcgccc gtttttgcgg
    17161 cgctaaaacc gtttatcgcc cgagcggggt taacgcccta caacgtggcg cgtaagcgtg
    17221 gcgaactgaa atacattctg ctgactgaaa gccagagtga tggcggcatg atgctgcgct
    17281 ttgtactgcg ttctgatacc aagctggcgc aactgcgtaa ggcgctgccg tggttacacg
    17341 aacaactacc gcagctgaaa gttattaccg tcaatattca gccggtacat atggcgatta
    17401 tggaagggga gacggagatc tacctgaccg aacaacaggc actggcggag cgttttaatg
    17461 acgtaccgct gtggatccgt ccgcaaagtt tcttccagac taatccggcg gtcgccagcc
    17521 agttgtacgc caccgcgcgc gactgggtac gacagctgcc ggttaaacat atgtgggatc
    17581 tgttctgcgg tgtggggggc tttggtttac actgcgcgac gcctgacatg cagttaaccg
    17641 ggatcgaaat tgcgccagag gccattgcct gtgcaaagca gtcagccgct gaactgggct
    17701 taactcgttt acaatttcag gcgctggact ccactcagtt tgccactgct cagggggagg
    17761 tgccggagct ggtgctggtt aacccgccgc gccgcggcat tggtaaaccg ctgtgtgatt
    17821 atctttcaac gatggcaccg cgttttatta tctactccag ctgtaacgcc caaaccatgg
    17881 cgaaagatat ccgcgaactg ccaggttacc gcatcgaacg ggtacagctt tttgatatgt
    17941 tcccgcacac cgcgcactat gaagtgttga cgctgctggt gaagcaataa aaaagccaca
    18001 ggtgcggctt cagattgctg atcaggtgcg ctttgttcat gccggatgcg gcgtgaacgc
    18061 cttatccggc ctacaaaatc atgctaaatc aatacattgc agagttgatg tgggccggat
    18121 aagcgcagcg catcaggcaa tcttactttt gtcatcagtc tcaattactg cggaaaccac
    18181 tggtcattga ttttctgata tgtaccgtca gctttaattg ctgccagcgc gttattcagt
    18241 ttttccagca gagctttgtt atccggacgt acagcgatac ccaggccggt gccgaagtat
    18301 tgcggatcgg tcactttttc agtcgcaacg cccagttgcg gattggtttt cagccattcg
    18361 tttaccaccg cggtgtcacc aaatacccca tcaatacgac catttttcag atcgataaag
    18421 gcattctgat aactgtcata ggagacagtt ttcacttccg ggtgctgatc ctgaatatat
    18481 ttctggtgcg tcgtaccgtt ttccatccca atgcgtttgc ctttcaggtc ggcaaacgtt
    18541 ttgtaggtat cttttttgga aatcacgacg gctgagtttt catagtaggg tgtggtaaac
    18601 gaaacctgct tgctacgctc cggggtgata tccataccgg agattacggc gtcatatttt
    18661 ctgaatttca gtgacgggat caggctgtcg aacgcgtgat tagtaaaagt acattctgcc
    18721 tgcatttgtt tgcacaaggc ttttgccaga tcgatatcaa agccgacaat ctcattatta
    18781 gcacctatag attcaaaggg tggataggtg gctgaaacgc caaaattgat tttctctgcg
    18841 gcagaagcac cgaaagtaaa ggaagcaagt aaagcggcaa gaactaactt tttcatgatg
    18901 gaactcccgt ctgtcaatct tatgattttt ggccgtgtct gcggcatggg ataacaatgc
    18961 catcaagtga atttatatgc aataaacatg attaaataat ttaaatgaaa taaaaaaaga
    19021 cggataactt agtgagttgt ccgtcttcat tataagaatt tatgcactat gtaggccgga
    19081 taaggcgtcc acgccgcatc cggcacaggc accgtgctga tgtctgatgc gacgctggcg
    19141 cgtcttatca gacctacaaa cccccccggc gaatgtacgc agctacatta attccgccgt
    19201 tcgaatgcca gtgctttgcg ctcgatcaga cgcatcatca gcgtcagcag gccgttgacg
    19261 accaagtaaa taatccctgc cgcaccgaac accattacat cgtaggtgcg tccgtacaac
    19321 aactggctgt atcccatcac ttccatcagc gtaatggtgt aagccagaga ggtacttttg
    19381 aacaccagca ccacttcgtt ggaataagaa gagagcgagc gtttaaaggc atacggcagc
    19441 aggatcgcca gcgtatcttt tttgctcatt cccagggcgc tacaggactg ccactgacct
    19501 tctgggatcg cacgaattgc accgtaaaac agctgcgtgg tatacgccgc actgttcagc
    19561 gataacgcaa tcagcgcaca taaccacggt tctgacaaca aatgccacag tgccggatac
    19621 tcctgcaacg tcggaaactg gcccggcccg taataaatca ggaagatctg caccagcagc
    19681 ggcgtaccgg taaacagcgt gatataaccc cgcaccagcc acaccagcac cggcgttttc
    19741 agcgtcagga tgatggtaaa aatcaatgcc agaatcagtg ccacaatcag cgaggcaacg
    19801 gttagcgtca ggctggtgtg cagccctttc atcagctcgg gtaaatactc aaacattagc
    19861 tgggcctccg ctcaaaacgt gtcgcgcgca ggtcaatgcg tttgagaatg tactgactga
    19921 gcagggtgat caccaggtaa atcgccgccg ccacaatgta ccaggtaaat ggttcctggg
    19981 tacgagtagc aatgcttttg gtttgtaaca ttaaatcatt cacactaatc aaactgacca
    20041 gcgcggtatc tttcagcagc accagccact ggttaccgag gccaggcagc gcatgacgcc
    20101 acatctgcgg catcaccaga cggaaaaaga tagccgattt cgacagcccc agcgcctgac
    20161 cagattccca ctggcccacc ggtacagctt tcaacgcgcc gcgcagcgtt tgcgaggcat
    20221 aggcggcata cagcagtgac agagcgatga caccacaaag gaacggactc acgtcgaagt
    20281 tctcaatgtc catctgcacc gggatctgca cgaacccaag attgatagtg aagccatccg
    20341 aaagcgtcag cagtaactgc gaggagccaa aatagataaa cagcactacc agaatttctg
    20401 gcaggccacg cagaatggtt accagcgctg aacctgccca cgcgacagga cgccatttcg
    20461 ctgactccca taccgcaaag aacatcgcca gcgccagccc gacaatcaat gcacaaacgg
    20521 caaggccgac ggtcatcccg gcggcgcttg ctaaaggaaa aaattcattc atcaggaatt
    20581 acttctggaa ccatttgttg tagatggttt cgtaagtgcc atctttcttc actttttcca
    20641 gcgcagtgtt gagtttctgc tgcagttcag tgtttccctg acgtaccgcg atgccgaggc
    20701 cagtaccgaa ataatcttta tcggtcactt tatcgcccac tgccgccagt tttgggttat
    20761 ctttcagcca ctcagtgacc actgcggtgt caccaaatac gctgtcgata cgcccgtttt
    20821 gcagatccag ttttgcgttc tggtagctgt catacggaac ggtggtgatt tccgggtgct
    20881 tatccataat gaacttctgg tgtgtcgtcc cgttctgaac gccgactttt ttgcctttca
    20941 gctgatcaac actggtgtat ttgccttgct gacccacaaa cagggcagag ttgtcatagt
    21001 acggggtagt aaacagtacc tgcttttcac gctccggagt gatatccatg cccgccatca
    21061 ctgcttctac gcgacggaat ttcaggcttg ggatcaggct gtcaaacgcc tggttagaga
    21121 aagtacaggt tgcatcaatc tctttacaca gtgcctgcgc caggtcgacg tcaaaaccaa
    21181 cgatctggtt gtttgcatca atcgattcaa acggaggata gggggcttcg gtagcaaaac
    21241 gaatggtttc ggcagctgtg gcggaaagac taaaacctgc aattaacgcg gcaatcagaa
    21301 cttttttcat tgttgttatc ccgaatctta gtgagagaga tagtttttaa atgcttcggt
    21361 ttgcggctcg gtaaagcagc tcgcgtcgcc ttgttctacg atatgaccat tttccatata
    21421 caccactcgg ctggcggttt tacgcgccac ttcaacttcg tgggtgacga tcacctgggt
    21481 aatattcgtt tctgccagct cacgaatgat gctgacgatt tgtgccgtaa tttccgggtc
    21541 cagtgcggcg gtcggttcat cgaacagcag tacctgcggt tccatcatca acgcacgggc
    21601 aatagcaaca cgctgctgct gaccaccaga aagatgcagc gggtaacgat cgctataagg
    21661 tttgagacgc agacgttcca gcagtttttc tgcacgggcc agcgcctgat ctttactcaa
    21721 ccccagtaca cggcagggcg cttcaatcag gttttgctgc acggtcagat gcggccacag
    21781 gttgtattgc tgaaacacca tgccaacgtt acgacgcaaa tcgcgaatcg ctttgtcaga
    21841 gggtgttttg gtgaaatcga aatggttgcc tgcaatgttg agcgtaccgg agcgcggcat
    21901 ctcaagcaga ttgagtacac gcagcagcga gcttttaccc gcgccgctgg ggccaagtaa
    21961 caccagcgtt tcgccctgtg ggcaatccag cgtgatatcg aacagcgcct gatgcgcgcc
    22021 gtagaagcaa ttaatgccgt ttaattgaat actcattgac actcgtatac tggcagtctg
    22081 atagctattg aggtcgaaga tagtaccttt aacagaataa ttatgcaata tttatgcttt
    22141 aaaagttaaa agcaaagcgc attattcaat aaacatagca caaaataacg ggggcggtgg
    22201 tcggtgagca taaatgtcgg cattcctcac gaaatgccgg ataatttacg gggtttattg
    22261 gttgatcaag gcgttagcga ttctcgatgg actgacggag cgtacccgcc gtggcatgaa
    22321 cgctaccgcc taagtagcgc acatcgtcga tgacccaaca ctggccttcc tgaatcatta
    22381 acacttcatc ctgccagccc tggtcaccct gtttgagatc cacccgcaat ggaatgttac
    22441 gggcatcacg attagggata gtcgatgcac tggcaacgtg ggcgctgtct ggcaaggtgg
    22501 ttcgactgga gaacggatcg ctgcttaaca attcacgatg actattatcg cggctggcat
    22561 cgctaagttg ggttgccagt ttgtcactca aataggggcg cagggcggta atgtcattgc
    22621 tgcgatgttg aatgcggtag tcgtagaatt gttgagccac actgtcaggc cctccctgga
    22681 cacaggaacc ggtgcggggg ccgttatctt tataagctgg cgtgacggtg gtacaggcac
    22741 tcagcagcag tgcacatgga ataagtagag atagtgaacg gtagcgcata atcatttcct
    22801 tattagcttg ctgtctgcgt cacactacga ccaaacgcta tcagattatt taatacattg
    22861 tttttaatac aattctgaaa caatctgaac acctgattgg gtttgagtcc attgctggcg
    22921 acccactcaa ggttgctcgt agctgactta cttaaattac ccaaacaata gacagtgtaa
    22981 accatttgtg aggttgagcg tgttatatgg ctgaaatgat catttaaggt aaatatgata
    23041 gttctgaaat ggtggtataa atcctcctgt agaggttaag agcatctgca agaagccagt
    23101 ttcacattta taacgcgtga taaatgaatg cggttattgt ctaatcatta caaaattttt
    23161 cacatgcgat aagagatcct gcgattaaga
//
LOCUS       DAZSIO010000051        23132 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZSIO010000051 DAZSIO010000000
VERSION     DAZSIO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23132
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.051"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            19..573
                     /locus_tag="ACHX5T_001835"
     CDS             19..573
                     /locus_tag="ACHX5T_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR8467067.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(583..2010)
                     /locus_tag="ACHX5T_001836"
     CDS             complement(583..2010)
                     /locus_tag="ACHX5T_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR8467068.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(2007..2714)
                     /locus_tag="ACHX5T_001837"
     CDS             complement(2007..2714)
                     /locus_tag="ACHX5T_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR8467069.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2878..3855
                     /gene="ybeQ"
                     /locus_tag="ACHX5T_001838"
     CDS             2878..3855
                     /gene="ybeQ"
                     /locus_tag="ACHX5T_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFR8467070.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(3925..4407)
                     /locus_tag="ACHX5T_001839"
     CDS             complement(3925..4407)
                     /locus_tag="ACHX5T_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR8467071.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            4642..7224
                     /gene="leuS"
                     /locus_tag="ACHX5T_001840"
     CDS             4642..7224
                     /gene="leuS"
                     /locus_tag="ACHX5T_001840"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR8467072.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7239..7820
                     /gene="lptE"
                     /locus_tag="ACHX5T_001841"
     CDS             7239..7820
                     /gene="lptE"
                     /locus_tag="ACHX5T_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR8467073.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            7820..8851
                     /gene="holA"
                     /locus_tag="ACHX5T_001842"
     CDS             7820..8851
                     /gene="holA"
                     /locus_tag="ACHX5T_001842"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR8467074.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8853..9494
                     /gene="nadD"
                     /locus_tag="ACHX5T_001843"
     CDS             8853..9494
                     /gene="nadD"
                     /locus_tag="ACHX5T_001843"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR8467075.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9518..10129
                     /locus_tag="ACHX5T_001844"
     CDS             9518..10129
                     /locus_tag="ACHX5T_001844"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFR8467076.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10389..10706
                     /gene="rsfS"
                     /locus_tag="ACHX5T_001845"
     CDS             10389..10706
                     /gene="rsfS"
                     /locus_tag="ACHX5T_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR8467077.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10710..11177
                     /gene="rlmH"
                     /locus_tag="ACHX5T_001846"
     CDS             10710..11177
                     /gene="rlmH"
                     /locus_tag="ACHX5T_001846"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR8467078.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11208..13109
                     /gene="mrdA"
                     /locus_tag="ACHX5T_001847"
     CDS             11208..13109
                     /gene="mrdA"
                     /locus_tag="ACHX5T_001847"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR8467079.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13112..14224
                     /gene="mrdB"
                     /locus_tag="ACHX5T_001848"
                     /gene_synonym="rodA"
     CDS             13112..14224
                     /gene="mrdB"
                     /locus_tag="ACHX5T_001848"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR8467080.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14235..15323
                     /gene="rlpA"
                     /locus_tag="ACHX5T_001849"
     CDS             14235..15323
                     /gene="rlpA"
                     /locus_tag="ACHX5T_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR8467081.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15462..16673
                     /gene="dacA"
                     /locus_tag="ACHX5T_001850"
     CDS             15462..16673
                     /gene="dacA"
                     /locus_tag="ACHX5T_001850"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR8467082.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16784..17047
                     /gene="ybeD"
                     /locus_tag="ACHX5T_001851"
     CDS             16784..17047
                     /gene="ybeD"
                     /locus_tag="ACHX5T_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR8467083.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17148..17789
                     /gene="lipB"
                     /locus_tag="ACHX5T_001852"
     CDS             17148..17789
                     /gene="lipB"
                     /locus_tag="ACHX5T_001852"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR8467084.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            18048..19001
                     /locus_tag="ACHX5T_001853"
     CDS             18048..19001
                     /locus_tag="ACHX5T_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR8467085.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            19210..20175
                     /gene="lipA"
                     /locus_tag="ACHX5T_001854"
     CDS             19210..20175
                     /gene="lipA"
                     /locus_tag="ACHX5T_001854"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR8467086.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20276..20479)
                     /gene="tatE"
                     /locus_tag="ACHX5T_001855"
     CDS             complement(20276..20479)
                     /gene="tatE"
                     /locus_tag="ACHX5T_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR8467087.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20608..21396)
                     /locus_tag="ACHX5T_001856"
     CDS             complement(20608..21396)
                     /locus_tag="ACHX5T_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR8467088.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            21489..21872
                     /gene="crcB"
                     /locus_tag="ACHX5T_001857"
     CDS             21489..21872
                     /gene="crcB"
                     /locus_tag="ACHX5T_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR8467089.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(21926..22135)
                     /gene="cspE"
                     /locus_tag="ACHX5T_001858"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21926..22135)
                     /gene="cspE"
                     /locus_tag="ACHX5T_001858"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR8467090.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22310..22870)
                     /gene="pagP"
                     /locus_tag="ACHX5T_001859"
     CDS             complement(22310..22870)
                     /gene="pagP"
                     /locus_tag="ACHX5T_001859"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR8467091.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         5977 a         5784 c         5696 g         5675 t
ORIGIN      
        1 aaatcgaagc attcacacat gaataaaaaa ttaatgtata tattcgcaat ttttatagtt
       61 gcagcaatta cctgtattag ccaacccaag aaaacgatgt tgcgtgataa agccatggtg
      121 aattatgcct ttgattattt aagatcacca ggcagtctac cgttaaccac ggcagccacg
      181 gagctttccg cgattcatgg tcactcaaca tcgcaatatc gccttggcga attttatctt
      241 catggtagcg acggtaaacc actggattat acacaggcga gatactggta tgagcaatca
      301 gcagaacagg aaaatccgcg cgcacaaagc aaactggggt ggatctacct gaaaggcctg
      361 ggagtcaaac ccgatacccg taaagcaatt ctctggtata aggaagcagc tgaacaaggg
      421 tatgctcatg ctcaatatac tttgggtttg atctacagaa atggcactgg tattaatgtt
      481 aaccattatg aatctcaaaa atggttaaaa ctggccgcca aacaacatta caaaaatgcg
      541 gaaagattac ttgccggact tcccgcacat taacgtcgac tgtcacccga agagatggag
      601 tattttccct atcaaaccgg caaagataaa aatgtagaaa ataattttga tgacgcccag
      661 acagcccagc ccgccatcat caacgggcat tggctgcggt tgcggctccg gctcgacggt
      721 ttcctgaccg gtaaaattcg ccgcctgaat taataatcga cgaataatta atgcttccag
      781 tggacaacga tcttctgcat caaggcggct cacaatcggc attaatgatt cttctgtcgg
      841 ttggctggcc tgcactaatt gtttggcaac taaatataat ggccataagg gatgttctgt
      901 atcacgcgtg agtgagatcc gcggcttacg tcctttgtgc aaaaccatct cgaccagtga
      961 acgccccagc gcagagagtt tttcatcgct gagtatttcg tataaatgaa aacgcgtacg
     1021 ggcgctgtcg ccggggacat aaagtagcgg ttttccctga tcgttaacca gatcacgcgc
     1081 ttcaatcatc atcaccacta atgggtaata atagacccga tgctgacggc accattgcaa
     1141 cattaaagca tccgcctggg tggagggata ctcgcgccag tagtaacaaa gttctgccag
     1201 caggctttcg ccttctccac aatagacgcg ttgtgcgtat tcataataac gcgcatcttc
     1261 attatcaggt tcagcttccc gccacgaccg ggcgtaatcc aggagttcgg ggattcccag
     1321 gcggcttgcg ctataccagc ggagtaaatt tctgtgcagt tttgcatcat cggggataat
     1381 cactgcgcga tgttgcgcta aatacaatgt cagccattct ggatgcgatg accaaatccg
     1441 agcgaacatt tggtaaaaat cgatggtgtc attttgcacg gcaactggca gatgcagaat
     1501 ggtctggaag ttaaaaacat tccccttcgc aatggcatat aaaaaggatt ccagttcttc
     1561 ttcgtctatt tccccgacgt tttcctcctg caaccaccgc aatctttctg ctaataacac
     1621 cacgcactcg aaggataaat gggcagtgtt catggcgatt gtgcacagcg accagcgtaa
     1681 ttcatcaatc tcttccatcg agcaataatt taattgctga atgaatcgct gccaggcgga
     1741 gggcagaaaa cgttcgctat cagaggcaag taacgcgcga aaggcgagca gaatttcatg
     1801 ttctgctacc tcatattctt ctggttgcca aacagattta gcaggcgact gcgcaatccg
     1861 tagcgcctcc tcatacgcct gacgaagttg tttaaaaccc tgcggatcgg tttctggatg
     1921 aaaggacggt aacagcgcca gataagcgcg gcggataata tcgacgtcag tcgtttcctc
     1981 tatatcgagg atcttccagc aatttttcat aagtcgctca tatccttaag cgtttccggg
     2041 catgtggggt agtcagccag tggttgccag gacaaggtag ggtaatacgc atcatcactg
     2101 gcaaataatt gataaaacat atcattgccg gaatcgtcgt attctttacg cgctaagaat
     2161 tcgcgtaata aaggcagatg atcatcttcg ggagactgcc aatattgccg accaaaggta
     2221 taaccggcaa agtattgccg ccagttacta taataacgta gagcccgtag atgaatacgc
     2281 gactggatcc atagactttc ttcttcactc aaccagttat taagtacgcc cattcgactt
     2341 aaaaaaccca tccggacata atcccatgct ttaattcctc cctcaccaca gcattcggct
     2401 gtgctggcga caaattgcgc ataagcctga ccttgctcat ttaattcagc aagatagtca
     2461 cgccattcac atggagaata acgaaaccag cggtgataaa accctgccaa ccgagcggca
     2521 tgaccgttct cgatcattcg gtgaattatg cgcagcaggt catcacggtc atcaattccc
     2581 catgaggaag aaaggtcaaa aaagcatgta cctaaataca ttttggggga tgaatgcgaa
     2641 ggtaaacagc attcattgcg tatttccatc ggagtggaaa gtgcaaacaa tattttttgc
     2701 gaccccatgt ccatataatt tatccttaaa cattcattta atgaatgtaa actacatttc
     2761 gaggctcagt tatttatact tttctgacgc aacataatac gcataaaata aggcttacgc
     2821 aaaaatggaa cacataaaaa tataatatat tttatctaca accataagga aatgataatg
     2881 atttttacgt caagttgctg cgataattta tcaatagatg agattatcga acgtgctgaa
     2941 aaaggcgatt gcgaggctca atatattgtt gggttttatt ataatcgcga tagcgcaatt
     3001 gactctccgg acgacgaaaa ggccttttac tggctgaagc tggccgctga gcaaggtcat
     3061 tgtgaagcac agtattcctt agggcaaaag tataccgagg ataaaagccg tcataaagat
     3121 aatgagcaag ccatcttctg gctgaaaaaa gctgccctac aaggccatac tttcgcttcc
     3181 aacgcccttg gctggatact ggatcgtgga gaagacccca actataaaga agcggttacc
     3241 tggtatcaga tagccgcgga gagcggaatg tcttatgcgc aaaataatct tggatggatg
     3301 tacagaaatg gcaacggagt cgcacaagac tatacgctgg catttttctg gtacaaacaa
     3361 gctgcattac aaggccatag ttacgcacaa gacaatctgg ccgatcttta taaagacgga
     3421 gaaggcgttg ctcaaaacaa gacactcgcc gcattctggt atttgaaaag cgcacagcag
     3481 ggtaatcggc acgcccagtt tcaaattgca tgggattata acgctggcga aggggtggac
     3541 caggactata agcaagcgat gtactggtat ctgaaggctg ccgctcagga aagcgtcggc
     3601 gcttacgtca acatcggtta tatgtataaa cacggacaag gcgttgagaa agattatcag
     3661 gctgcctttg aatggtttac gaaagccgct gaatgcaatg acgccactgc ctggtataac
     3721 ctggccatta tgtatcacta cggagaagga agacctgtcg atctccgaca ggctctcgac
     3781 ctgtatcgta aagttcagtc atccggaacc agagatgtca gtcaggaaat tcgtgagact
     3841 gaagctttac tgtagaaata aaaacaaggg ccggggaatt tcattctgcc catattctct
     3901 ttattgctgt gcgaaatcag actattacgg ctcaaacggg cgtctctgga actggtcata
     3961 accacatttc gggcatagcg tcagcacttc cggtgtgtag atcgggagat ggaagtgaca
     4021 tttctcgcag accagatttc ccagcccgac cacttctccg ctgtgataaa ccccatgatg
     4081 attgaggtcc tggaaaattt cgcgccattc aagctgcgtt ttatcggtga tgtctgccag
     4141 ctcctgccac aagctttctt taatcacccg cataaagacg ctgtcagatt cttctttcag
     4201 gctctcttca tagctcatgg cgaactcttc caggtcacgt ctgacagctc gcgtcagctc
     4261 atcgatctcg gttcgcgtta actcccctgt ttttattacg cgctcgcgcg cctgttccac
     4321 cagtgcgtcg atatcacgtt cgccattgcg caggcgttcg ttcagtgacg cgaccagttc
     4381 acggtaatat tgagcaacct tgttcatcgt ttcatctccc gggtcgttaa ctgactataa
     4441 taatagacgt tctttgttcc ttgctttgct aatacgccca caaactctgt aaattaatgt
     4501 acgaataatc cgggcatcat ttcaacgtcg agtgcgcgaa aggctgtttt gacgcggacg
     4561 tttgggctat gctatgcgga tctgaaaaac cacctcaacg ctacatttgt agccgtattg
     4621 aaaacaggac cactggctgc catgcaagag caataccgcc cggaagagat agaatccaaa
     4681 gtacagcttc attgggatga gaagcgcaca tttgaagtaa ccgaagacga gagcaaagag
     4741 aagtattact gcctgtctat gcttccctat ccttctggtc gactacacat gggccacgta
     4801 cgtaactaca ccatcggtga cgtgatcgcc cgttaccagc gcatgctggg caaaaacgtt
     4861 ctgcagccga tcggctggga cgcgtttggt ctgcctgcgg aaggcgcggc ggtgaaaaac
     4921 aacaccgctc cggcaccgtg gacgtacgac aacatcgcgt atatgaaaaa ccagctcaaa
     4981 atgctgggct ttggttatga ctggagccgc gagctggcaa cctgtacgcc ggaatactac
     5041 cgttgggaac agaaattctt caccgagctg tataaaaaag gcctggtata taaaaagact
     5101 tctgcggtca actggtgccc gaacgaccag accgtactgg cgaacgaaca agttatcgac
     5161 ggctgctgct ggcgctgcga taccaaagtt gaacgtaaag agatcccgca gtggtttatc
     5221 aaaatcactg cttacgctga cgagctgctc aacgatctgg ataaactgga tcactggcca
     5281 gacaccgtta aaaccatgca gcgtaactgg atcggtcgtt ccgaaggcgt ggagatcacc
     5341 ttcaacgtta acgactatga caacacgctg accgtttaca ctacccgccc ggacaccttt
     5401 atgggttgta cctacctggc ggtagctgcg ggtcatccgc tggcgcagaa agcggcggaa
     5461 aataatcctg aactggcggc ctttattgac gaatgccgta acaccaaagt tgccgaagct
     5521 gaaatggcga cgatggagaa aaaaggcgtc gatactggct ttaaagctgt tcacccatta
     5581 acgggcgaag aaattcccgt ttgggcagca aacttcgtat tgatggagta cggcacgggc
     5641 gcagttatgg cggttccggg tcacgaccag cgcgactacg agtttgcctc taaatacggc
     5701 ctgaacatca aaccggttat cctggcggct gacggctctg agccagatct ttctcagcaa
     5761 gccctgactg aaaaaggcgt gctgttcaac tctggcgagt tcaacggtct tgaccatgaa
     5821 gcggccttca acgccatcgc cgataaactg actgcgatgg gcgttggcga gcgtaaagtg
     5881 aactaccgcc tgcgcgactg gggtgtttct cgccagcgtt actggggcgc gccgattccg
     5941 atggtgacgc tggaagacgg taccgtaatg ccaaccccgg acgaccagct gccggtgatc
     6001 ctgccggaag atgtggtgat ggacggcatt accagcccga ttaaagcaga tccggagtgg
     6061 gcaaaaacta ccgttaacgg tatgccagcg ctgcgtgaaa ccgacacttt cgacaccttt
     6121 atggagtcct cctggtacta tgcgcgctac acttgcccag agtacaaaga aggtatgctg
     6181 gattccgaag cggctaacta ctggctgccg gtggatatct acattggtgg tatcgaacac
     6241 gccattatgc acctgctcta cttccgcttc ttccacaaac tgatgcgtga tgcaggcatg
     6301 gtgaactctg acgaaccagc gaaacagttg ctgtgtcagg gtatggtgct ggcagatgcc
     6361 ttctactatg ttggcgaaaa cggcgaacgt aactgggttt ccccggttga tgctatcgtt
     6421 gaacgtgacg agaaaggccg tatcgtgaaa gcgaaagatg cggcaggcca tgaactggtt
     6481 tataccggca tgagcaaaat gtccaagtcg aagaacaacg gtatcgaccc gcaggtgatg
     6541 gttgaacgtt acggcgcgga caccgttcgt ctgtttatga tgtttgcttc tccggctgat
     6601 atgactctcg aatggcagga atccggtgtg gaaggggcta accgcttcct gaaacgtgtc
     6661 tggaaactgg tgtacgagca cacagcaaaa ggtgatgttg cggcactgaa cgttgatgcg
     6721 ctgactgaag atcagaaagc gctgcgtcgc gatgtgcata aaactatcgc taaagtgacc
     6781 gatgatatcg gccgtcgtca gaccttcaac accgcaattg cggcgattat ggagctgatg
     6841 aacaaactgg cgaaagcacc gaccgatggc gagcaggatc gcgctctgat gcaggaagca
     6901 ctgcaggccg ttgtccgtat gctcaacccg ttcaccccgc acatctgctt cacgctgtgg
     6961 caggaactca aaggcgaagg cgatatcgac aacgcgccgt ggccggttgc tgacgaaaaa
     7021 gcgatggtgg aagactccac gctggtcgtg gtgcaggtta acggtaaagt ccgtgccaaa
     7081 atcaccgtcc cggtggacgc aacggaagaa caggttcgcg aacgtgctgg ccaggaacat
     7141 ctggtagcaa aatatcttga tggcgttact gtacgtaaag tgatttacgt accaggtaaa
     7201 ctcctcaatc tggtcgttgg ctaagcgcgg gaggaagcgt gcgatatctg gcaacactgt
     7261 tgttatctct ggcggtgtta atcaccgccg ggtgtggctg gcatctgcgt gacaccacgc
     7321 aggttccttc cactatgaag gtcatgatcc tggactcagg cgatccgaac gggccattaa
     7381 gccgtgcggt gcgtaaccag ttacgtctga atggtgtcga gttgcttgat aaagaaacca
     7441 cgcgtaagga cgttccatcc ctgcgtttgg gtaaagtgag catcgcgaaa gataccgcat
     7501 cggtattccg taacggtcaa acagcagagt atcagatgat catgacggtg aatgcgaccg
     7561 tgttgatccc cggccgtgat atctacccga ttagcgccaa agtcttccgt tcattcttcg
     7621 ataacccgca aatggcgtta gcgaaagata acgaacaaga catgatcgta aaagagatgt
     7681 acgaccgtgc tgccgaacag ctgattcgta agctgccaag catccgtgct gcggatattc
     7741 gttccgacga agaacagacg tcgaccacaa cggatactcc ggcaacgcct gcacgcgtct
     7801 ccaccatgct gggtaactga tgattcggtt gtacccggaa caactccgcg cgcagctcaa
     7861 tgaagggctg cgcgcggcgt atcttttact tggtaacgat cctctgttat tgcaggaaag
     7921 ccaggacgct gttcgccagg tagctgcggc acaaggattc gaagaacacc acactttttc
     7981 cattgatccc aacactgact ggaatgcgat cttttcgtta tgccaggcta tgagtctgtt
     8041 tgccagtcga caaacgctat tgctgttgtt accagaaaac ggaccgaatg cggcgatcaa
     8101 tgaacaactt ctcacactca ccggacttct gcatgacgac ctgctattga tcgtccgcgg
     8161 taataaatta agcaaagcgc aagaaaatgc cgcctggttt actgctcttg cgaatcgcag
     8221 cgtgcaggtg acctgtcaga caccggagca ggctcagctt ccccgctggg ttgctgtgcg
     8281 cgcaaaacag ctcaacttag aactggatga cgcggcgaat caggtgctct gctactgtta
     8341 tgaaggtaac ctgctggcgc tggctcaggc actggagcgt ttatcgctgc tctggccaga
     8401 cggcaaactg acattaccgc gcgttgaaca ggcggtgaat gatgccgcgc atttcacccc
     8461 ttttcattgg gttgatgctt tgttgatggg aaaaagtaag cgcgcgttgc atattcttca
     8521 gcaactgcgt ctggaaggca gcgagccggt tattttgttg cgcacattac aacgtgaact
     8581 gttgttactg gttaacctga aacgccagtc tgcccatacg ccactgcgtg cgttgtttga
     8641 taagcatcgg gtatggcaga accgccgggg catgatgggc gaggcgttaa atcgcttaag
     8701 tcagccgcag ttacgtcagg ccgtgcaact cctgacacga acggaactca ccctcaaaca
     8761 agattacggt cagtcagtgt gggcagagct ggaagggtta tctcttctgt tgtgccataa
     8821 acccctggcg gacgtattta tcgacggttg atatgaaatc tttacaggct ctgtttggcg
     8881 gcacctttga tccggtgcac tatggtcatc taaaacccgt ggaaacgctg gcgaatttga
     8941 ttggtctgac gcgggtcaca atcatcccta ataatgttcc tccgcatcgt ccccagccgg
     9001 aagcgaacag cgtgcagcgt aaacacatgc ttgaactggc gattgccgac aagccattat
     9061 ttactcttga tgaacgcgag ctaaagcgca atgccccctc ttacactgcg caaacactga
     9121 aagagtggcg gcaggaacaa ggaccggacg tgccgctggc gtttattatt ggtcaggatt
     9181 cattgctgac ctttccgacc tggtacgaat acgaaacgat actcgacaat gcacatttga
     9241 tcgtctgtcg gcgtccaggt tacccacttg aaatggcgca accgcaatac cagcaatggc
     9301 tggaagatca tttgacacat aacccggaag atcttcacct tcagcctgcc ggtaaaattt
     9361 atctggctga aacgccgtgg tttaacatct cggcgaccat catccgcgaa cgtttgcaaa
     9421 acggtgaatc gtgtgaggat ttattgccgg aaccggtatt gacttacatt aaccaacaag
     9481 gcttgtatcg ctgatacctg tcgttacgga gggcatgatg cgactgtggt taattcgtca
     9541 tggcgaaaca caagcgaatg tcgatggtct gtacagcggt catgcgccca cacccctgac
     9601 cgcacgcggt attgagcaag cgcaaaatct gcatacactg ctagatgatg tttcctttga
     9661 cttggtttta tgcagtgaac tggaacgggc acagcatacc gcgcgactgg ttctcagtga
     9721 ccgccagctc cccgttcata tcatccctga actcaacgaa atgttttttg gcgactggga
     9781 gatgcgacat catcgcgacc tcatgcaaga agatgccgaa aactatagcg cgtggtgcaa
     9841 tgactggcag catgctatcc ccacgaacgg tgaaggattt caggcatttt cgcaacgtgt
     9901 ggaacgcttt atcgcaaggc ttagtgaata tcagcactat cagaatattt taatcgtcag
     9961 ccatcagggc gtactgagtc tgttaattgc tcgtttaatt ggcatgcctg ccgagtctat
    10021 gtggcatttt cgcgttgacc aggggtgttg gagcgccatt gatatcaacc aaaaattcgc
    10081 gacgctacgc gtcctcaata gccgtgccat cggggtcgag aacgcatgac ttttctgttt
    10141 ttttacgggt aagccgcaac gaccattgac agccccgggc aggctgatat tctccgcagc
    10201 cagacttttt ccgccagaca cgactttgta gaaattgttt tacaaaaatg gcgatgcaat
    10261 ctgcggcgcg gggtgggatg atagcccact ttcgaaagcc gattcggcga caattgtccc
    10321 gaaatcgcct ctggttcagg tatactgaca gaccattttt atctatttga ttcacccagg
    10381 gggaaaactt gcagggtaaa gcactccagg attttgttat cgacaaaatt gatgacctca
    10441 aaggtcagga catcatcgcc ttagacgttc aaggcaaatc cagcatcacc gactgcatga
    10501 tcatctgtac gggtacgtcc agccgtcatg ttatgtccat tgctgaccac gttgtgcagg
    10561 agtctcgcgc agcgggcctg ttaccgctcg gcgtagaagg tgaaaacagc gccgactgga
    10621 ttgtcgtgga tttgggcgat gtgattgtcc atgtcatgca ggaagagagc cgtcgcctgt
    10681 atgaactgga aaaactctgg agttaatgcg tgaagctgca acttgtcgcc gtgggaacga
    10741 aaatgccgga ctgggtacaa accggtttta ccgagtacct gcgtcgtttt ccgaaagata
    10801 tgcccttcga gctgattgaa attccggccg gcaaacgcgg caagaatgcg gacatcaagc
    10861 gcatactcga caaagagggt gagcagatgt tggcggccgc aggcaaaaac cgcattgtca
    10921 ccctcgatat tccaggcaag ccctgggata cgccgcagtt agccgctgag ctggaacgct
    10981 ggaagctgga tggtcgcgac gtaagtctac tgattggcgg gcctgaaggg ttgtcgcctg
    11041 cctgtaaagc ggcggctgag cagagctggt cgctgtcggc gcttaccctc ccccatccgc
    11101 tggttcgcgt gctggtcgca gagagtctgt accgggcgtg gagcatcacc accaaccatc
    11161 cttatcaccg tgagtgataa gggagctttg agtagaaaac gcagcggatg aaactacaga
    11221 actcttttcg cgactatacg gcagagtccg cgctgtttgt gcgccgggcg ctggtcgcct
    11281 ttttggggat tttgctgctg accggcgtgc ttatcgccaa cctgtataat ctgcaaattg
    11341 ttcgctttac cgactaccag acccgctcta atgaaaaccg cattaagctg gtgcctatcg
    11401 cgcccagccg cggcattatc tacgaccgta acggtatccc tctggccctc aaccgcacta
    11461 tctaccagat agaaatgatg ccggagaaag tcgataacgt gcagcaaacg ctggacgctt
    11521 tgcgcagcgt ggtagatctg accgatgacg atattgctgc attccgaaaa gagcgcgcac
    11581 gttcacaccg tttcacctct attccggtga aaactaacct aaccgaagta caagtagctc
    11641 gctttgccgt caatcagtac cgttttccgg gtgtcgaagt taaaggctat aaacgtcgtt
    11701 actatcctta cggttcggcg ttgacccacg tcatcggcta tgtgtcgaaa atcaacgata
    11761 aagacgtcga acgcctgaat aatgacggca aactggccaa ctatgcggca acgcatgata
    11821 ttggtaagct gggcattgag cgttactatg aagacgtgct gcacggtcag accggttatg
    11881 aagaggttga agttaacaac cgtgggcgtg ttatccgcca gttaaaagaa gtaccaccgc
    11941 aagccggaca cgatatttac ctgacgctgg atctcaaact ccagcaatat attgaaacgc
    12001 tgctggcggg tagccgcgca gctgtggttg ttaccgaacc gcgtacaggt ggggtgctgg
    12061 cgctggtttc cacgcctagt tatgacccaa acctgtttgt tgacggtatc tccagcaaag
    12121 attattccgc cttgctgaac gacccgaata caccgctggt gaaccgcgcc acacaggggg
    12181 tttatcctcc cgcgtctaca gttaaaccct atgtggcggt ttcggcatta agcgccgggg
    12241 tgatcacgcg caatacgacg ctgtttgacc caggctggtg gcaactacca gggtcggaaa
    12301 aacgttatcg tgactggaaa aaatggggcc acgggcgtct gaatgtcaca agatcgctgg
    12361 aagaatctgc ggataccttc ttctatcagg tggcctacga tatggggatc gatcgcctct
    12421 ccgaatggat gggtaaattc ggttatggtc attacaccgg tatcgacctg gcggaagaac
    12481 gttccggcaa catgcctacc cgcgaatgga aacagaaacg ctttaaaaaa ccgtggtatc
    12541 agggtgacac cattccggtt ggtatcggcc agggttactg gacagcgacc ccaatccaga
    12601 tgagtaaggc actgatgatc ctgattaatg acggtatcgt gaaggttccg catttgctga
    12661 tgagcaccgc cgaagacggc aaacaggtgc catgggtaca accgcatgaa ccgcccgtcg
    12721 gcgatattca ttccggttac tgggagctgg cgaaagacgg tatgtacggt gttgctaacc
    12781 gccctaatgg tacggcgaat aaatactttg ccagcgcacc gtacaaaatt gcggcgaaat
    12841 ctggtaccgc tcaggtcttc ggtctgaaag cgaacgaaac ctataatgcg cacaaaattg
    12901 ccgagcgttt acgtgaccac aaactgatga ccgcctttgc gccatacaac aatccgcaag
    12961 tggctgtcgc catgattctg gagaacggcg gcgcgggtcc ggcggttggt acgctgatgc
    13021 gccagatcct cgaccacatt atgctgggtg ataacaacac cgatctgcct gcggaaaatc
    13081 cagcggttgc cgcagcggag gaccattaat catgacggat aatccgaata aaaaaacatt
    13141 ctgggataaa gtccatctcg atcccacaat gctgctgatt ttactggcat tgctggttta
    13201 cagcgccctg gttatctgga gcgccagcgg tcaggatatt ggcatgatgg agcgtaaaat
    13261 cggccaaatc gcgatgggtc tggtcatcat ggtggtgatg gcgcaaattc ctccacgcgt
    13321 ttatgaaggc tgggccccct atctctatat catctgtatt attttgctgg tggcggtaga
    13381 tgctttcgga gccatctcta aaggcgctca acgctggctg gacctcggta ttgttcgttt
    13441 tcagccgtcg gaaattgcca aaatagccgt accactgatg gttgcgcgct ttatcaaccg
    13501 cgacgtttgc ccgccatcgt tgaagaatac tggcatcgcg ctggtgctga tatttatgcc
    13561 cacgctgctg gtggctgcac agcctgacct gggaacatca atcctcgttg cgctttccgg
    13621 tctgtttgta ctgttcctct ctggccttag ctggcgtctg attggcgtcg cagtagtgct
    13681 ggtagcggcg ttcattccga ttctgtggtt cttcctgatg catgattacc agcgccagcg
    13741 cgtaatgatg ctcctggacc cggaatcaga cccactcggc gcgggctatc acattattca
    13801 gtctaaaatt gctattggct ccggcggatt acgcggcaaa ggctggctgc acggcactca
    13861 gtcacagctt gaatttctcc ccgaacgcca tactgacttt atcttcgcgg tactggcgga
    13921 agagctggga ttagtgggca ttctgattct gctcgctctc tacattctgc tgatcatgcg
    13981 cgggctgtgg atagccgcca gagcgcaaac cacctttggt cgcgtcatgg ctggtggctt
    14041 aatgctgata ttattcgttt atgtcttcgt aaatattggt atggtaagcg gtattctgcc
    14101 ggttgtaggg gttccgctcc cactggtcag ttatggagga tcggcgctaa ttgtgctgat
    14161 ggctgggttc gggattgtaa tgtcaatcca cacccacagg aaaatgttgt cgaaaagcgt
    14221 gtaagaggtg cgcaatgcgt aagcagtggc tcgggatctg catcgcggca ggaatgctcg
    14281 cggcatgtac aagcgatgat ggtcagcaac agacggtaag tgtaccgcag cctgcggtat
    14341 gtaacggccc catagttgaa attagcgggg cggacccgcg tttcgaacca ctgaacgcga
    14401 cggcaaatca ggattaccag cgcgacggta aaagctacaa aatcgtgcag gatccgtctc
    14461 gatttagcca ggcgggactg gcggcaatct atgatgccga accaggcagt aacctgacgg
    14521 cctctggcga agctttcgat ccgacaaagc tgacggcggc ccatccaacg cttccgatcc
    14581 ccagctacgc cagaatcact aacctggcta acgggcgaat gatcgtggtg cgcattaatg
    14641 atcgcggccc ttacggcaac gaccgcgtta tttcgctttc tcgcgcggca gctgaccgtc
    14701 ttaacacgtc aaacaacacc aaagttcgta tcgatccgat tattgtcgcc caggatggtt
    14761 cgctttctgg tcctggtatg gcttgtacca cagtcgccaa acagacttac gccctgcctg
    14821 cacctcccga tttaagcggt ggcgcgggaa caagttcagt gtctggcccg cagggtgaca
    14881 ttcttccggt cagtaattcg acgctaaaaa gcgaagatcc gaccggcgcg ccggtaacca
    14941 gtagcggttt cctcggcgct ccaacgacct tagcgcctgg tgtactggaa ggcagcgaac
    15001 cgacgcctgc gccacagccc gttgttacag cttcgtcgac aacgcctgca acctcgcctg
    15061 caatggtgac accgcaagcc gcctcgcaaa gcgccagcgg caactttatg gtgcaagtcg
    15121 gggccgtaag cgatcaggct cgtgcgcaac agtaccaaca gcaactggga cagaagttcg
    15181 gcgtccccgg tcgcgtaact caaaatggcg cggtctggcg gatccagctt ggcccattcg
    15241 ccagcaaagc cgaagccagt accttgcagc aacgtttgca aaccgaagcc caattacagt
    15301 catttattac taccgcgcag tagcgtaaag caggcatctg atgtgtcaat ttctgtaaat
    15361 gacgttaaca aagtcacgcg gaaagtcaga tgcctgccgg tagtgcattt gctatagtag
    15421 ggcacttttt taattccatc acggatgtcg tagttcagac catgaatacc attttttccg
    15481 ctcgtatcat gaagcgcctg gcgctcacca cggctctttg cacagccttt atctctgctg
    15541 cacatgccga tgacctgaat atcaaaacta tgatcccggg tgtaccgcag atcgatgcgg
    15601 agtcctacat cctgattgac tataactccg gcaaagtgct cgccgaacag aacgcagatg
    15661 tccgccgcga tcctgccagc ctgaccaaaa tgatgaccag ttacgttatc ggccaggcaa
    15721 tgaaagccgg taaatttaaa gaaactgatt tagtcactat cggcaacgac gcatgggcca
    15781 ccggtaaccc ggtgtttaaa ggttcttcgc tgatgttcct caaaccgggc atgcaggttc
    15841 cggtttctca gctgatccgc ggtattaacc tgcaatcggg taacgatgct tgtgtcgcca
    15901 tggccgattt tgccgctggt agccaggacg cttttgttgg cttgatgaac agctacgtta
    15961 acgcactggg cctgaaaaat acccacttcc agacggtaca tggtctggat gctgatggtc
    16021 agtacagctc cgcgcgagat atggcgctga tcggccaggc attgatccgt gacgtaccga
    16081 atgaatactc gatctataaa gaaaaagaat ttacgtttaa cggtattcgc cagctgaacc
    16141 gtaacggcct gttatgggat aacagcctga atgtcgacgg catcaaaacc ggacacactg
    16201 acaaagcagg ttacaatctt gttgcttctg cgactgaagg ccagatgcgc ttgatttctg
    16261 cggtgatggg tggacgtact tttaaaggcc gtgaagccga aagtaaaaaa ctgctaacct
    16321 ggggcttccg tttcttcgaa accgttaacc cactgaaagt aggtaaagag ttcgcctctg
    16381 aaccggtttg gtttagtgat tctgatcgcg cttcgttagg ggttgataaa gacgtgtacc
    16441 tgaccattcc gcgtggtcgc atgaaagatc tgaaagccag ctatgtgctg aacagcagtg
    16501 aattgcatgc gccgctgcaa aagaatcagg tcgtcggaac tatcaacttc cagcttgatg
    16561 gcaaaacgat cgagcaacgc ccgctggttg tgttgcaaga aatcccggaa ggtaacttct
    16621 tcggcaaaat cattgattac attaaattaa tgttccatca ctggtttggt taaaaattga
    16681 acacttgaaa gtgtaatttc cgtccccata tactaagcat cagtaaaaaa actcccgcct
    16741 tctggcggga gttgctattt aattacgtta cgccggagct gacatgaaaa ccaaacttaa
    16801 cgaactgctt gaattcccta ctccttttac ttacaaagtt atggggcagg cgttacctga
    16861 gctggttgat caggtggttg aagtggtaca gcgccatgcg ccaggtgact acaccccaac
    16921 ggtaaaacca agcagcaaag gcaactacca ctcggtatct atcactatca acgccactca
    16981 tatcgagcag gttgaaacac tgtatgaaga actgggcaaa atcgatattg tccgcatggt
    17041 tctgtaactc gcttctccgt tacccggctc ctggtcgggt aactcccctc tcctcgctgt
    17101 gatatacttt tcccccactt ttactcattc tccacggaga tgccgttttg tatcaggata
    17161 aaattcttgt ccgccagctc ggtcttcagc cttacgagcc aatctcccgg gctatgcatg
    17221 aattcaccga tacccgcgat aatagtaccc ttgatgaaat ctggctggtc gagcactatc
    17281 cggtattcac ccaaggtcag gcaggaaaag cggagcacat tttaatgccg ggtgatattc
    17341 cggtgatcca gagcgatcgc ggtgggcagg tgacttatca cgggccggga caacaggtga
    17401 tgtatgtgtt gcttaacctg aaacgccgta aactcggtgt gcgtgaactg gtgaccttgc
    17461 ttgagcaaac agtggtgaat accctggctg aactgggtat agaagcgcat cctcgggctg
    17521 acgcgcctgg tgtctatgtc ggggaaaaga aaatttgctc actgggttta cgaattcgac
    17581 gcggttgttc attccacggt ctggcattaa acgtcaatat ggatctttca ccatttttac
    17641 gtattaatcc ttgtgggtat gccggaatgg aaatggctaa aatatcacaa tggaaacccg
    17701 aagcgacgac taataatatt gctccacgtt tactggaaaa tattttagcg ctactaaaca
    17761 atccggactt cgaatatatt accgcttaat tccatacatc aatggcccaa tttacactgg
    17821 gtcattactc ataatacctg ctaatgcttt acatctcctt cgcgtttgta tattttcttt
    17881 atcacttgca gaaatgcggt gccacgcctt gctccgtaac tcaccccatt gattaaccta
    17941 ttgaaatagt tgattatggt gagcaaaagc gttccgttcc aattagacat aagattattg
    18001 taatttttta aatattattt ttccatgaat aaaattggag taagtgcgtg gatagtaata
    18061 atcaaattga accctgttta agcagaaagt cgtcagaagg taaaccacaa atatttacaa
    18121 ccttacgaaa tattgatctt aaccttctga ctatttttga agctgtatat gtacataaag
    18181 ggatcgttaa tgcagcgaaa gtgcttaatc tgaccccctc ggcaatcagt cagtctattc
    18241 agaaactgcg cgttatattc cctgacccat tgtttattcg caaaggccag ggtgtcactc
    18301 ctaccgcatt tgcgatgcat ctacatgagt atatcagtca gggccttgag tccattcttg
    18361 gcgcgctgga tatcgaagga agctatgata agcaacgaac gataactatt gcgaccactc
    18421 cctcggtcgg agccctggtc cttcctgtca tctatcgggc gattaaaact cactatccgc
    18481 agcttttact gcgcaacccg cccatcagcg acgcggaaaa ccaactcagt cagtttcaaa
    18541 ccgatctcat catcgataac atgttttgca ccaatcgtac ggtgcaacat catgttctgt
    18601 tcaccgacaa tatggtgtta atttgccgca agggaaatcc actactctct ttagaagatg
    18661 acagagagac tatcgacaac gctgcgcatg tacttctgtt accggaaggg caaaatttca
    18721 gcggtctgcg gcagagagtt caagagatgt ttccggaccg gcaaatcaat ttcaccagct
    18781 acaacatttt gacaatcgct gcactggttg ccaacagtga catgttagcg attattccca
    18841 gccgttttta taacctgttt agtcgttgct ggccgctgga aaaactgcct tttccgtcct
    18901 taaatgagga gcaaatagat ttctctatcc actacaacaa attcatcctg cgtgatccca
    18961 tcctgcacgg agtaatagat gttatccgta atgcattttg aaaaagtatt gttacagcca
    19021 gatcacagcg cagaacgata aaatggcaca atccaacaac aattttacat tttcgcgacc
    19081 gctttggctg ctttcaggtc cgtttcaatg atatactgcc agtcgttaat tcaaaaatag
    19141 ttgataatta caacaatcta ttgaattgaa acgctttcct tcgtaattcg caactggaac
    19201 acgcacgcta tgagtaaacc cattgtgatg gaacgcggtg ttaaataccg cgatgccgat
    19261 aagatggccc ttatcccggt taaaaacgtg gcaacagagc gcgaagccct gctgcgcaag
    19321 ccggaatgga tgaaaatcaa gcttccagcg gactctacac gtatccaggg catcaaagcc
    19381 gcaatgcgca aaaatggcct gcattctgtc tgcgaggaag cctcctgccc taacctggcg
    19441 gaatgcttca accacggcac agcaacgttt atgatcctcg gcgctatttg tacccgccgt
    19501 tgtccgttct gtgatgttgc ccacggtcgc ccggtagctc ctgatgccaa tgaaccagtg
    19561 aaactggcgc agaccattgc cgatatggcg ctgcgttatg tggttatcac ctccgttgac
    19621 cgtgatgacc tgcgcgatgg cggtgcccaa cactttgcgg attgtattac tgccattcgg
    19681 gaaaaaagcc cgcaaatcaa aattgaaact ctggtgccgg atttccgcgg tcgaatggat
    19741 cgtgctctgg atattctgac tgcaacgccc ccagatgtgt tcaaccataa cctggaaaac
    19801 gtaccgcgta tttaccggca ggtacggcct ggcgcagatt acaactggtc gctgaagctg
    19861 ctggaacgct ttaaagaagc gcatccggaa atcccgacca agtctggtct gatggtgggt
    19921 ctgggtgaaa ccaatgaaga aattattgag gtaatgcgcg acctgcgccg tcatggtgtg
    19981 acgatgttaa cgctggggca atatttgcag ccaagccgcc atcacctgcc ggttcaacgt
    20041 tacgttagcc cggatgagtt cgacgaaatg aaagccgaag cgctggcgat gggctttacc
    20101 catgctgcat gcggtccgtt tgtccgctct tcttaccacg ccgatttgca ggcgaaagga
    20161 atggaagtta agtaatcctg taataattac ttacatctgt ctgataaaaa acccgctttt
    20221 tgaagcgggt tttttgtatc gaacaagata ttgagggagc gtcctgctcg ccacgtcact
    20281 ctttatgaga gagcttttca gcctgaagat caacgtctgc gccttttttc gccgcagcat
    20341 cgtcatcatt catcgccttc ttgaaccctt taatggccgc tccaaggtct ccgcccagcg
    20401 tacgtaactt cttagtccca aacagcagaa cgaccagcgc cgcaactacc agcagtttgg
    20461 taatactaat ctcacccata gataccttct tgacatataa ccggcaacag aacgcgcctt
    20521 aaaaaccaga gaatattaac ggtcattcga cgcgcgcaca caatagcaat ctgtaacaag
    20581 gtgaatcaag cgccgagtaa aaaaacatca taataattgc ggcggcgcaa agcgacggtt
    20641 gtttaagatg ggcagttgcg cgcgcacctg acgcacacgt tcgggcgtca cttccgccat
    20701 aatgagtgca ggcatttctg acgctgccgc aatggtgacg ccaaagggat ctataatccg
    20761 gctttgcccg atatttttgt tcccgcactc ccccgccgcc accatataac aggtggtatc
    20821 cagcgcacga gcggcaagca acgttgacca gtgatgctct ttgagcggcc cacgaaccca
    20881 ggcggcagga agtaccagga tttcagctcc ctgtaaagcc tgtgccagcg ccagctctgg
    20941 aaagcgtaag tcataacagg tcatcagacc gaccttcatc ccttccacct ccagtaacgg
    21001 agctatttca ttaccagcat caacatggcg tgattcctga atggcaaatg catcatagag
    21061 atgcagtttg gcataacggg cgacgatgtt tcctgcctga agtgccacca gcatattcca
    21121 tgcgcgcccc ggcgttgaag gaagatgaat cgtcagaatt gtcgtcatca tgttacgttt
    21181 actttctcgc cgtaaacgtc cgaggaattc gccttccagc agctgtgctg atttaaccga
    21241 tagatctgca tcatgatcat cgcgcgccag caatgcttcc ggcaggacaa acagcgatac
    21301 gtcgttttcc gccgcctgcg ccatcaacga ggcacaaatc tcagcgttct tttcccacac
    21361 agatgtaaca gcaaactgtc ctgcagcaac cagcattgcc acctcctgtc agagtcgtaa
    21421 aattcgccaa cgacgattat tcggcgttac acttatcact catacaaatc aaatagcagg
    21481 attttgcagt gttacaactt cttttagcag tttttattgg cggtggtacg ggaagcgtag
    21541 cgagatggct gttaagtatg cgatttaacc cgctgcatca ggcgattccg ttggggacgc
    21601 tggcagcaaa cctgattggg gcattcatca taggaatggg attcgcctgg ttcagcagga
    21661 tgacgaacat tgatccagtg tggaaagtat taatcaccac cggattttgt ggcggtctaa
    21721 caaccttctc aacattttcg gcagaagtgg tgtttttgtt acaagagggc cgctttggct
    21781 gggcattact gaacgttttc gtcaaccttc tggggtcttt tgccatgacc gcactggcat
    21841 tctggctgtt ttcggcctca accgtacact aaagaccaaa aaaaacccgc tgattaagcg
    21901 ggttttgaat tcttgctgac gtatcttaca gagcgattac gtttgcagca gaagggcctt
    21961 tggcaccgtt agtgatttcg aactctacgc gctgaccttc agcaagagtt ttaaaaccat
    22021 tagtctggat tgcagagaag tgtacgaaca cgtctttgct gccgtcttcc ggagtaatga
    22081 aaccgaatcc tttggactca ttaaaccact taacgttacc tttaatctta gacatcaaaa
    22141 ttacctttac atgaaaaata gacacaaatg ctgtgtcggt tatcagtaca ccaattgtgg
    22201 tacgctttgt ccagtgcaac tttactaaaa agtgataaaa gtcgcgttat ttttgatgtt
    22261 gcttatttta gttattgatt ttaaagaagt tactaaaact tcatttgtct caaaactgaa
    22321 agcgcatcca ggcaaagtac acattgccat tgttgtaggt acccggaatg taggtcatct
    22381 gaaaagtcac tgggccataa cccacggagg ccaatggcag tagaaccggg agagggatgt
    22441 aattccagtt atcgcgtgcc gttacgccag cggtgaatcc cagacctaaa tgaaaatttt
    22501 catccgccag cggtcgccag gtactttccc atccgtatcc ggcaatcggt tcccatttgt
    22561 tccacgagtc cttaaatgcc atggcataca ggccatgcca gtttcctttt tcatcccaac
    22621 gcgacaggcc aaaaccgcca ccccacggtc gctcgttata gcgatcggtt ttttctttgt
    22681 cgtaagcgaa acgtgcatgc caggtgatgg caggaatata taaatcataa tgttcaggct
    22741 gttgccaggt ttgtgcaata ttttctctaa acgttgtcat cctctcatct gcgttagcaa
    22801 aaactttacc aacgctgatt aactgaataa aaacaaagga aaagatagcg acatatttac
    22861 tcacgttcat ttgtgaccat aaaacattta tcaaaaatct actactagca tagcaaagct
    22921 acaattaaca taaccttaat agacccaaca taaagaataa tctgaatagc tgctctattt
    22981 atttccgctt gttcaagtta aatatttccg cgcatcgatt aaagatgatg cgtcgttttc
    23041 tgcgatggga atagtcaaaa aagaaaaacc aagtcttttt tgatgacaaa atgcaatcaa
    23101 ggaaaaatta ttttattttt ttaacgagat ac
//
LOCUS       DAZSIO010000052        22594 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZSIO010000052 DAZSIO010000000
VERSION     DAZSIO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.052"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            137..1720
                     /locus_tag="ACHX5T_001860"
     CDS             137..1720
                     /locus_tag="ACHX5T_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR8467092.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(2172..4025)
                     /gene="asmA"
                     /locus_tag="ACHX5T_001861"
     CDS             complement(2172..4025)
                     /gene="asmA"
                     /locus_tag="ACHX5T_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR8467093.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4047..4628)
                     /gene="dcd"
                     /locus_tag="ACHX5T_001862"
     CDS             complement(4047..4628)
                     /gene="dcd"
                     /locus_tag="ACHX5T_001862"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR8467094.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4720..5361)
                     /gene="udk"
                     /locus_tag="ACHX5T_001863"
     CDS             complement(4720..5361)
                     /gene="udk"
                     /locus_tag="ACHX5T_001863"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR8467095.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5679..5921
                     /locus_tag="ACHX5T_001864"
                     /pseudo
     CDS             5679..5921
                     /locus_tag="ACHX5T_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            5920..6861
                     /gene="mdtA"
                     /locus_tag="ACHX5T_001865"
                     /pseudo
     CDS             5920..6861
                     /gene="mdtA"
                     /locus_tag="ACHX5T_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            6861..9983
                     /gene="mdtB"
                     /locus_tag="ACHX5T_001866"
                     /pseudo
     CDS             6861..9983
                     /gene="mdtB"
                     /locus_tag="ACHX5T_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            9984..13049
                     /gene="mdtC"
                     /locus_tag="ACHX5T_001867"
     CDS             9984..13049
                     /gene="mdtC"
                     /locus_tag="ACHX5T_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR8467096.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            13050..14465
                     /locus_tag="ACHX5T_001868"
     CDS             13050..14465
                     /locus_tag="ACHX5T_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8467097.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            14462..15865
                     /gene="baeS"
                     /locus_tag="ACHX5T_001869"
     CDS             14462..15865
                     /gene="baeS"
                     /locus_tag="ACHX5T_001869"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR8467098.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            15862..16584
                     /gene="baeR"
                     /locus_tag="ACHX5T_001870"
     CDS             15862..16584
                     /gene="baeR"
                     /locus_tag="ACHX5T_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR8467099.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            16775..17107
                     /locus_tag="ACHX5T_001871"
     CDS             16775..17107
                     /locus_tag="ACHX5T_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR8467100.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            17254..18615
                     /gene="yegQ"
                     /locus_tag="ACHX5T_001872"
     CDS             17254..18615
                     /gene="yegQ"
                     /locus_tag="ACHX5T_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR8467101.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(18957..19274)
                     /gene="yegR"
                     /locus_tag="ACHX5T_001873"
     CDS             complement(18957..19274)
                     /gene="yegR"
                     /locus_tag="ACHX5T_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFR8467102.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            19680..20579
                     /gene="yegS"
                     /locus_tag="ACHX5T_001874"
     CDS             19680..20579
                     /gene="yegS"
                     /locus_tag="ACHX5T_001874"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR8467103.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNSLIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(20661..21434)
                     /locus_tag="ACHX5T_001875"
     CDS             complement(20661..21434)
                     /locus_tag="ACHX5T_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR8467104.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(21540..22580)
                     /gene="gatD"
                     /locus_tag="ACHX5T_001876"
     CDS             complement(21540..22580)
                     /gene="gatD"
                     /locus_tag="ACHX5T_001876"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR8467105.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         5214 a         6033 c         5897 g         5450 t
ORIGIN      
        1 taattcaggg ataatgtgag gttattaccc tcaaataata tgagataaat agtgctgcaa
       61 cattgcattt ttgccccgat ttatccatga tcgaattgtg acatttgtca tacaacgaat
      121 aggttttgta cttactatgg aatggattgc cgatccgtct atctgggccg ggttaatcac
      181 gctgattgtg atcgaactgg tcctcggcat tgataacctg gtctttattg ccatcctcgc
      241 cgaaaaacta ccgccgaagc agcgcgaccg cgcacgggtt accgggctgc tgctggcgat
      301 gttaatgcgc ctgttactgc tagcgtcaat ctcctggctg gtcaccctga ctcaaccgct
      361 gttcagcttc cgctcgttta cctttagcgc ccgcgactta atcatgctgt ttggtggttt
      421 cttcctgttg ttcaaagcca cgatggagct gaacgaacgg ctggaaggga aagacagcaa
      481 taatcccaca caacgcaaag gtgcgaagtt ctggggcgtg gtgacgcaaa ttgtggtact
      541 ggacgccatt ttctcactcg actcagtgat taccgccgtc gggatggtcg accatttact
      601 ggtcatgatg gccgccgtgg ttatcgctat cagcctgatg ttgatggcca gcaagccgtt
      661 aacgcaattt gttaacagtc acccgaccat cgttattctc tgcttaagct tcctgttgat
      721 gattggcttt agcctggtgg cagaaggttt cggcttcgtc attccgaaag gctacctgta
      781 cgctgccatt ggtttctcgg tgatgatcga ggcactcaat cagttggcta tctttaaccg
      841 gcgacgtttt ctttccgcta atcagacgct acgccagcgg actaccgaag cggtaatgcg
      901 cctgcttagc gggcaaaaag aagatgcgga actggacgcc aaaaccgcgt ccatgctggt
      961 ggatcatgga aaccagcaga tctttaatcc gcaggaacgg cggatgattg agcgggtact
     1021 taatcttaac cagcgtaccg taagcagcat tatgacgtcg cgccacgata ttgagcatat
     1081 cgatctcaac gcgccggaag aagaaatccg ccagttgctg gagcgaaatc agcatacgcg
     1141 gctggtggtt accgacggtg atgacgcaga agatttgctc ggtgttgttc acgttatcga
     1201 cctgctacaa cagtcactgc gcggcgaacc gctcaacctg cgggtgttga ttcgccagcc
     1261 gctggtgttc ccggaaacct tgccgttgtt accagccctg gagcagttcc gtaatgcccg
     1321 cacgcacttt gcttttgtgg tggatgagtt tggctcggtg gaagggattg tgacattaag
     1381 tgacgtcact gaaaccattg ccggtaactt accgaacgaa gtggaagaga tcgacgcccg
     1441 ccatgatatt cagaagaatg cggacggttc ctggacggcg aatggtcata tgccgctgga
     1501 ggatctggtg caatatgtgc cgctgccgct ggatgaaaaa cgtgaatatc acaccattgc
     1561 cgggctgttg atggagtatt tgcagcgtat tccaaaacct ggcgaagaag ttcaggtggg
     1621 ggattatttg cttaaaacgt tgcaggtgga aagccatcgc gtgcagaagg tgcagattat
     1681 accgctgcgt aaggatggcg agatggagta cgaggtgtga tggtatcggc cattttaccc
     1741 aatgctgcgt aacgccttat aacttcagca atgaagcgtt gtctgtggta aacggacatc
     1801 acagcggggg tgtgggtccg gctgtaaatc gccgaatcgt ttcctgtagg ctggggcagc
     1861 ccacatggat gtgggctgag ggcgcgtttt acagggatgt tacctcgcgc ccgtacccga
     1921 tagccgaaag gaataagatg agggaaccgc gccagcggcg atttaccgcc gggagcccgg
     1981 gttgccaggg tggtggcgat tgagccaccc tggcacgttc accggcttga gcgttccata
     2041 gtagcgagga acatcaagtg aacggaacag ccttcgatct tacatattca gaatataata
     2101 gtgccgccgc gaacgcctga tgcgacgctt tacgcgtctt atcaggccta caggacaggt
     2161 cacctggtaa cctacatctt ctccagcaac ttcttcacat ctttgccatt gcgggaatct
     2221 ttattccgct cggcccagtc attcaggcga cgtttcgctt cgtcctgtag atgtttgcgc
     2281 agtaactgat ccacttgcag gctgtaattg agttgctgcc agttgccata aacccgcagc
     2341 ggtactggcg tttctttgag gaaatcaatc agtttgcttt ccccgttcca gccacccacg
     2401 acccgaatat caaactgggt gtcgcaggtt tgatctgcca gattcaacat gccttcccct
     2461 gtcagcgcca gcacaggcga ttgaccttgc atgtcgttta acgtcacgac gccatccttc
     2521 aacgtcaaat cggtggtaaa gcggtcaagc cgcgtcacgt tatcgaagtt ttcagcggcc
     2581 ttcacatcac cgccattacg ctctaccgct tgctgaatca tctgctggaa gttcatccct
     2641 tccatacgtg tgtcggtcat ctcaacatgc gcctgtcctt gccagttgtg gcggaatgcg
     2701 tcggcatcta tgtcagcacc ggagaaatca ccagccagtg acatttttcc ggtcaacgaa
     2761 atcggatagt taaacgcctt cagaatggta ccaatctcaa cgttctccag ccgtggctgg
     2821 aagtttatcc gcggatttat tgatgtcgcg tccagcgtgc ccggcagtga aacctttcca
     2881 ccgttaagct tgccctgcag ttgagtaatt tccagcaaac ccgacttgtt ggtcatttgc
     2941 gtggcaacat ctgtaaaatt cattccgcgc cagcgcacgt tactggcctg caacaaaata
     3001 tcagctgtaa agccttgcag tccctgatag gccggttcat caatacgcga agaaatgacc
     3061 gggcgcggca acgttgattg gctctgcccc tgctgcgcgg caccgttttc accattcgcg
     3121 gtttcattaa gcgggatgag gttgtcaaga ttcagttgcg ggaattgcag tctcagctgc
     3181 cattccggtt tctctgtcag cgtgacctgt gcttgtccgc tcagcgtact gtcattggcg
     3241 gtcaaactaa tttggttaaa tgaaaggcgt ttatgtgact cctgccactg ggactggaaa
     3301 ctcccctgcc cctgaatacc ttgttttggt aaatcggcac cctgcaactg ccagttaatt
     3361 tgttcaatag ccgctgttaa atcatgcgga taatcagaag catctaccgt accgttaagg
     3421 gatatcgtga gatcgcgctg atcgcgatta acccgcccgg agaactcaaa tgagccacga
     3481 tgttggggat cttgttccat ttgcaggcgg atattgcgga ttgtcacctg ctcgtcatct
     3541 tcatgctgga acaccagaac gctgtccgcc accttaagac tggatatatc aaacgaccat
     3601 ccgcgatcgt ctgacagatc cggcaaggta ttgtcgcgcg gtgcaaccgg agcgtcttca
     3661 ctgcgcaccg cttccgtctg cggcgtcagt tggatcactg ccccttttag catcacctgc
     3721 ttaacgctca gttgatgact cagtagtggt aaaagcgcca cgtccagacg catgttgtcg
     3781 gcgcgaacca gtggctggct tgcgccctgg gcggtgagag acattcgccc ggagaggata
     3841 ctaagctgcg gccagacgtg ccaacgcagt ggcccgtcga gctgcaattg ataaccgcta
     3901 cgtgcagcaa cttgcttgac catatagtcg cggaaatcat tcggattcac cagcaacact
     3961 aacgcagata acccggccac cagcacgacc aagagtatca tcagcgtcgt cagaaatcgt
     4021 ctcatggtat cctcaatggg cctgaattag tctttatcga ttcggctggc taccgcgccc
     4081 tgctggttgc gatatttcgc atcttcacgg cggttgtaag gtcgcgccgc cgggccggaa
     4141 agcggctcaa agctcagcgc accaattaac atgcccggac gcagcgccag cggcagctta
     4201 ccggagttgt agaactccag cacaatgcaa ccagaccagc ccggatcgat acggtgcgcg
     4261 gtgacgtgca ccatcagccc cagacgcgcc aaagaggaac gcccgtccag ccagcccacc
     4321 agatcggctg gcagcgtcac cgactccagt gtcaccgcca gcgccagctc tcctgggtga
     4381 agatagaacg cctcgccctc gtcgagaacg atctcatcgc tcatcacgcg gtcaagcgcg
     4441 gcgctcactt catctttggg accgctcaga tcgataaacg ctgccgtgtg accacggaag
     4501 gtacgaaatt tattgcccag gcgtacatcc accgtcgcgc cgttaatacg ctccactggc
     4561 ggacgtgggt tgatcgacaa acggccttca tcaagccagg cttcaatatc tcggtcacac
     4621 agacgcatgg cattttctcc tttcgcacat cactccctta acgccaatta cgtcaagggc
     4681 ataccaactt atcactgaac ggtacacaat ttatcaagct tattcaaaga actgacttat
     4741 tttcgctttc aatatatcga tcgcgatgcg gtttttcccg ccgcgcggca cgataatgtc
     4801 cgcatattgt ttagaaggct caatgaattg caggaacatc gggcgcacgg ttttttgata
     4861 ttgcgccatc acggaatcca ttgaacgccc acgctcgtta acgtcacgct tgatgcggcg
     4921 catcaggcag atatccagcg gggtatcaac gaaaatggag aagttaagtt cgtcacgcaa
     4981 acgcgcatcc gtcagcaaca aaatgccttc gagaatgatg accttcttcg gctcaaccgt
     5041 caccgtttct ttcatacgcg tatgttcaac atagctgtaa accggcaggt caattgccga
     5101 gccgcgtttc aacgcttgta aatgctcaag caacaggctg tgatccatcg cgctgggatg
     5161 gtcgtagttg gtcttaacgc gttcttccat cgacagatgg ctttgatctt tgtaatagca
     5221 gtcttcggga attacgccga tgtgttcatc accgacttgc tcacgtaatt cacgataaag
     5281 ggtactggca ataagactct tgccggaagc cgatgcgcca gcgataccga taatgacgca
     5341 ctgatgagac tgatcagtca tatatttagc gacctgatta acctggatgt taggaagggg
     5401 gcgacgaagc gccaaacgcg gcaattatag ggatttcatc cgtctgatac cagtcgaata
     5461 gcgttgccgc gcgctcagag ttaattgttg acaaagaatt cccggtggca aattacgttg
     5521 atcagtttta tgtaaggtaa aaaatgttat acgcagttgc gcaaattatc cgcctttacg
     5581 tcactttatg agcaattcgc atataaaatg taaaactttt gtactagcat aaacacagaa
     5641 acgaatactg gcgacctggc cttgcggata aagcggtaat aagcaaacaa tcacagcatg
     5701 tattaattgc cctgccccac ccgctgcttc acctggtcag tttaggttta gtctcgttta
     5761 tctttaccct tgtctcgctt gagctttcgc agtttggcac ccaactcgcc ccactgtggt
     5821 tcccgacgtc catcatgatg gtggcgtttt atcgccatgc cgggcgcatg tggccgggaa
     5881 ttgcgctgag ctgttcgctg ggaaatatcg ccgcatccat tccaggaagg ccagcaggtc
     5941 aaagcaggcg atttactggc agaaattgac cccagccagt tcaaagttgc attagcacaa
     6001 acccagggcc aactggcaaa agataaagcc acgcttgcta acgcccgccg tgatctggcg
     6061 cgttatcagc aactggcaaa aaccaatctc gtctcccgtc aggagctgga tgcccaacag
     6121 gcgctggtca gtgaaaccga aggcaccatt aaggctgatg aagcaagcgt cgccagcgcg
     6181 cagttgcaac tcgactggag ccgcatcacc gcaccagtcg atggtcgcgt tggtctcaag
     6241 caagttgatg ttggtaacca aatctccagt ggtgatacca ccggaattgt ggtgatcacc
     6301 cagacgcatc ctatcgattt agtctttacc ctgccggaaa gcgatatcgc tactgtagtg
     6361 caggcgcaaa aagccggaaa accgctgatg gtagaagcct gggatcgcac caattcgaag
     6421 aaattaagtg aaggcacgct gttaagcctc gataaccaaa tcgatgccac aaccggtacg
     6481 attaaagtga aagcacgctt taataatcag gatgatgcgc tgtttcccaa tcagtttgtt
     6541 aacgcgcgca tgttagtcga caccgaacaa aacgccgtag tgatcccaac agccgccctg
     6601 caaatgggca atgaaggcca ttttgtctgg gtgctgaata gcgaaaacaa ggtcagcaaa
     6661 catctggtga cgccgggcat tcaggacagt cagaaagtgg tgatccgtgc aggtatttct
     6721 gcgggcgatc gcgtggtgac agacggcatt gatcgcctga ccgaaggggc gaaagtggaa
     6781 gtggtggaag cccagagcgc caccactccg gaagagaaag ccaccagccg cgaatacgcg
     6841 aaaaaaggag cacgctcctg atgcaggtgt tacccccgag cagcacaggc ggcccgtcgc
     6901 gcctgtttat tatgcgtcct gtcgccacca cgctgctgat ggtggcgatc ttactcgccg
     6961 ggattatcgg ttatcgcgcc ctgcccgttt cggcgctgcc ggaagtggac tatccgacca
     7021 ttcaggtggt cacgctctac ccaggtgcca gcccggatgt catgacctct gccgttaccg
     7081 cgccgctcga acgccagttc gggcagatgt ctggcctgaa acagatgtcg tcgcaaagtt
     7141 ccggcggtgc gtcagttatc actttgcagt tccagctaac attaccgctc aatgtcgccg
     7201 agcaggaagt gcaggccgcg attaacgctg cgaccaactt gttgccgagc gatctgccta
     7261 acccgccggt ttacagcaaa gtgaacccgg cagatccgcc gatcatgacg ctcgccgtca
     7321 cctcaaccgc catgccgatg acgcaagtgg aagatatggt ggaaacccgc gtcgcgcaga
     7381 aaatctcgca gatttccggc gtcggcctgg tgacgctttc cggcggtcag cgtccggctg
     7441 ttcgcgtcaa acttaacgct caggcgattg ccgccctcgg cctgaccagc gaaaccgtgc
     7501 gcaccgccat taccggcgct aacgttaact cggcaaaagg tagcctcgac ggcccttccc
     7561 gtgcggtcac gctttccgcg aacgaccaga tgcaatccgc cgaagagtat cgccagctaa
     7621 tcatcgccta ccagaacggc gcgccaattc gtctgggcga tgtcgcaact gtagagcaag
     7681 gtgcagaaaa cagctggctc ggcgcgtggg cgaacaaaga acaggccatt gtgatgaatg
     7741 ttcagcgcca gcccggtgct aacattatct ccaccgccga cagcattcgg cagatgctgc
     7801 cacagctcac tgagagtctg ccgaaatcgg tgaaggtgac agtgctttcc gatcgcacca
     7861 ccaatatccg cgcatccgtc gatgatactc agtttgaatt gatgatggct atcgcgctgg
     7921 tagtcatgat tatctacctg tttttgcgca atattccggc gaccatcatt cccggtgttg
     7981 ctgtaccgct gtcgttaatc ggcactttcg cggttatggt gtttctcgat ttttcaatca
     8041 ataacctgac actgatggct ttaactatcg ccaccggatt cgtggtcgat gacgccatcg
     8101 tggtgatcga aaacatttcc cgctatatcg aaaaaggcga aaaaccgttg gcggcggcgc
     8161 tcaagggcgc aggtgaaatc ggctttacca ttatctcgct gaccttctca ctgattgcgg
     8221 tgttgatccc actgctgttt atgggcgata tcgtcgggcg actgttccgc gaatttgcta
     8281 ttaccctggc ggtagcgatt ttgatctcag cggtggtgtc gctgaccctg acaccgatga
     8341 tgtgcgcgcg gatgctcagc caggagtcgt tgcgtaaaca gaacagcttc tcccgtgcct
     8401 cggaaaaaat gttcgacagg ataatcgccg cctatggtcg tggactggcg aaagtgctga
     8461 atcatccgtg gctgacctta agcgtggcac tcagcacgct gctgcttagc gtgctgctgt
     8521 gggtgttcat tccgaaaggt ttcttcccgg tacaggacaa cggcattatt cagggcactt
     8581 tgcaggcacc gcaatccagc tcctttgcca atatggccca gcgacaacgc caggtcgcgg
     8641 acgtgatttt gcaggatccg gcagtgcaaa gcctgacctc atttgttggc gttgatggca
     8701 ctaacccgtc gctgaatagt gcacgtttac aaatcaacct caaaccgttg gatgaacgtg
     8761 atgatcgggt gcaaaaagtc atcgcccgtc tgcaaacggc ggtagataaa gtgccgggcg
     8821 tcgatctctt cctgcaacca acgcaggacc tgaccatcga tactcaggtc agccgcaccc
     8881 agtaccagtt taccttgcag gccacgtcac tggatgcgct cagtacctgg gtgccacagt
     8941 tgatggaaaa actccagcaa ctgccacagc tttctgatgt ctccagcgac tggcaggaca
     9001 aagggctggt ggcgtatgtc aatgttgatc gcgacagcgc cagccgtctg gggatcagca
     9061 tggcggacgt cgataacgcc ctgtacaacg cgtttggtca gcgactgatt tccactattt
     9121 atactcaggc taaccagtat cgcgtggtgc tggagcacaa taccgaaaat accccaggcc
     9181 tcgcggcgct ggataccatt cgcctgacca gcagcgacgg tggcgtggtg ccgttaagct
     9241 caattgccaa aattgagcag cgttttgcgc cgctttccat caaccatctg gatcagttcc
     9301 cggtaacaac catctccttt aacgtgccgg ataactattc gctgggcgat gcggtgcagg
     9361 cgattatgga caccgaaaag acgctgaatc tgccggtgga tatcaccacg caattctagg
     9421 gcagcaccct cgccttccag tcggcgctgg gcagcactgt ctggctgatt gtcgcggcgg
     9481 tggtggcgat gtatatcgtg ctcggcattc tgtacgagag ctttattcac ccgatcacca
     9541 ttctctcgac gctacccacc gcaggggtcg gcgcgctgct ggcattaatg attgccggta
     9601 gcgaactgga tgtgattgcg attatcggca ttattttgct gatcggtatt gtgaagaaga
     9661 acgccatcat gatgatcgac ttcgcgctgg ctgctgagcg cgagcaaggc atgtcgccgc
     9721 gcgatgcaat ctaccaggct tgtctgttgc gttttcgtcc gatcctgatg accaccctgg
     9781 cggctctgct tggcgcgctg ccgctgatgt tgagtaccgg ggtcggcgcg gaactgcgtc
     9841 gtccgttagg tatcggcatg gtcggcggtc tgattgtcag ccaggtgctg acgctgttca
     9901 ccacgccggt gatttatctg ctgttcgacc gcctggcatt gtggaccaaa agtcgctttg
     9961 ctcgccatga agaggaggcg taagtgaagt tttttgccct cttcatttac cgcccggtgg
    10021 cgacgatttt actgtcggtc gccattaccc tgtgcggcat actgggtttc cgtatgctgc
    10081 cggtcgcccc gctgccgcag gtcgattttc cggtgattat ggtcagcgcc tcgctgcccg
    10141 gtgcgtcgcc ggagacaatg gcgtcttccg ttgccacgcc gctggagcgc tcacttgggc
    10201 gcattgccgg ggttagtgaa atgacctcca gcagttcgct cggcagcacg cgcattattt
    10261 tgcagtttga ttttgaccgg gatatcaacg gcgcagcgcg tgatgtgcag gcggcgatca
    10321 acgctgcaca aagtttgctg cccagcggga tgcccagccg cccgacctat cgcaaagcga
    10381 acccgtcgga tgcgccaatt atgatcctca cgctgacatc cgatacttat tcgcagggtg
    10441 aactgtacga tttcgcctcg acgcagctgg ctccgacgat ttcgcaaatc gacggtgtcg
    10501 gtgatgtcga tgtcggtggc agctcgctgc ccgccgtgcg cgtcgggctg accccgcagg
    10561 cgctgtttaa tcagggcgtg tcgctggacg acgtacgcac tgccatcagc aatgccaacg
    10621 tgcgtaaacc gcagggggcg ctggaagatg gcactcaccg ctggcagatc cagaccaacg
    10681 atgaactgaa aaccgccgct gaatatcagc cgctgattat tcactacaac aacggcggcg
    10741 cggttcgtct gggcgatgtg gcgacggtga ctgactcagt gcaggatgtg cgcaacgccg
    10801 ggatgaccaa cgccaaaccg gctattttac tgatgatccg caaactgccg gaagccaata
    10861 ttatccagac ggtagacagc atccgggcaa aattaccaga gttgcaggag accattccgg
    10921 cggcgattga tctgcaaatt gcccaggatc gctcccccac cattcgcgcc tcgctggaag
    10981 aagtcgagca aacgctgatt atctcggtgg cgctggtgat tctggtggtc tttttattcc
    11041 tgcgctcggg tcgcgccact attattcccg ccgttgcggt gccggtttcg ctgattggta
    11101 cgtttgcggc gatgtacctg tgcggattca gtctcaataa tctttcgctg atggcgctca
    11161 ccattgctac tggtttcgtg gtggatgacg ccattgtggt gctggaaaac attgcccgtc
    11221 atctggaagc ggggatgaaa ccactacagg ccgcgctgca aggtactcgc gaagtcggct
    11281 ttacggtgct gtcgatgagt ctgtcactgg tggcggtgtt cctgccgctg ctgttgatgg
    11341 gcggattgcc gggccgactg ttacgcgagt ttgccgtgac gctttctgtc gccattggta
    11401 tttcgttgct ggtttccctg acgctaacgc caatgatgtg cggctggatg ctaaaagcca
    11461 gcaagccgcg tgagcaaaag cgactgcgtg gttttggccg catgttggta gccctgcaac
    11521 aaggctacgg caagtcgctg aaatgggtgc tcaatcatac ccgtctggtg ggcgtggtgc
    11581 tgcttggcac cattgcgctg aatatctcga tcccgaaaac cttcttcccg gagcaggaca
    11641 ccggcgtact gatgggtggg atacaggcgg atcagagtat ttcgtttcag gcgatgcgcg
    11701 gcaaattgca ggatttcatg aaaattatcc gtgacgatcc ggcagtggat aatgtcaccg
    11761 gctttaccgg cggttcacga gttaacagtg ggatgatgtt tatcaccctc aagccacgcg
    11821 acgaacgcag cgaaacggcg cagcaaatta tcgaccgtct gcgcgtgaag ctggcgaaag
    11881 aaccgggggc gaatctgttc ctgatggcgg tacaagatat tcgcgttggt gggcgtcagt
    11941 cgaacgccag ctaccagtac acgttgttat ccgacgacct ggcggcactg cgcgaatggg
    12001 agccgaaaat ccgcaaaaaa ctggcgacgt tgccggaact ggcggacgtg aactccgatc
    12061 agcaggataa cggcgcggag atgaatctgg tttacgaccg cgacaccatg gcacggctgg
    12121 gaatcgacgt acaggccgcc aacagtctgt taaataacgc cttcggtcag cggcaaatct
    12181 cgaccattta ccagccgatg aaccagtata aagtggtgat ggaagtggat ccgcgctata
    12241 cccaggacat cagtgcgctg gaaaaaatgt tcgttatcaa taacgaaggc aaagcgatcc
    12301 cgctgtcgta tttcgctaaa tggcaaccgg cgaatgcccc actatcggtg aatcatcagg
    12361 gattatcggc ggcctcgacc atctcgttca acctgccgac cggaaaatcg ctctcggacg
    12421 ccagtgcggc gatcgatcgc gcaatgaccc agcttggtgt gccttcgacg gtgcgcggca
    12481 gttttgccgg cacggcgcag gttttccagg agacgatgaa ctcgcaggtg atcctgatta
    12541 tcgccgccat cgccacggtg tatatcgtgc tgggtatcct ttacgagagt tacgtacatc
    12601 cgctgacgat tctctccacc ctgccctcgg cgggcgttgg agcgctgttg gcgctggagc
    12661 tgttcaatgc cccgttcagc ctaatcgccc tgatagggat catgctatta atcggcatcg
    12721 tgaagaaaaa cgccattatg atggtcgatt ttgcgcttga agcccaacgg cacggtaacc
    12781 tgacgccgca ggaagctatt ttccaggcct gtctgctgcg ttttcgcccg attatgatga
    12841 ccaccctggc ggcgctgttt ggcgcgctgc cgctggtatt gtcgggcggt gacggctcgg
    12901 agctgcggca acccctggag atcaccattg tcggcggtct ggtgatgagc cagctcctga
    12961 cgctgtatac cacgccggtg gtgtatctct ttttcgaccg tctgcggctg cgtttttcgc
    13021 gtaaacctaa acaaacggta accgagtaaa tgacagatct tcccgacagc acccgctggc
    13081 aattatggat tgtggctttc ggcttcttta tgcagtcgct ggacaccacc atcgtaaaca
    13141 ccgcccttcc ctcaatggcg caaagcctcg gggaaagtcc gttgcatatg cacatggtca
    13201 ttgtctctta tgtgctgacc gtggcggtga tgctgcccgc cagcggctgg ctggcggaca
    13261 aagtcggcgt gcgcaatatt ttctttaccg ccatcgtgct gtttactctc ggttcactgt
    13321 tttgcgcgct ttccggcacg ctgaacgaac tgttgctggc acgcgcgtta cagggcgttg
    13381 gcggtgcgat gatggtgccg gtcggcaggt tgacggtgat gaaaatcgta ccgcgcgagc
    13441 aatatatggc ggcgatgacc tttgtcacgt tacccggtca ggtcggtccg ctgctcggtc
    13501 cggcgctcgg cggtctgctg gtagaatacg cgtcgtggca ctggatcttt ttgatcaaca
    13561 ttccggtggg gattatcggt gcgatcgcca cattgatgtt aatgccgaac tacaccatgc
    13621 agacgcggcg ctttgatctt tccggctttt tattgctggc ggttggcatg gcggtattaa
    13681 ccctggcgct ggacggcagt aaaggtacag gtttatcgcc gctggcgatt gctggcctgg
    13741 tcgcagttgg cgtggtggca ctggtgcttt atctgctgca cgccagaaat aacaaccgcg
    13801 ccctgttcag tctgaaactg ttccgcactc gtaccttttc gctgggcctg gcggggagct
    13861 ttgccggacg tattggcagt ggcatgttgc cctttatgac accggttttc ctgcaaattg
    13921 gcctcggttt ctcgccgttt catgccggac tgatgatgat cccgatggtg cttggcagca
    13981 tgggaatgaa gcgaattgtg gtacaggtgg tgaatcgctt tggttatcgt cgggtactgg
    14041 tagcgaccac gctgggtctg tcgctggtca ccctgttgtt tatgactacc gctctgttgg
    14101 gctggtacta cgttttgccg ttcgtcctgt ttttacaagg gatggtcaac tcgacccgtt
    14161 tctcctccat gaacaccctg acgctgaaag atctcccgga caatctggcg agcagcggaa
    14221 acagcctgct gtcgatgatt atgcaattgt cgatgagtat cggcgtcacc atcgccgggc
    14281 tgttgctggg gctttttggt tcgcagcata tcagcgtcga cagcggcacc acacaaaccg
    14341 tctttatgta cacctggctt agcatggcgt ttatcatcgc ccttccggcg ttcatctttg
    14401 ccagagtgcc gaacgatacg catcaaaatg tagctatttc gcggcgaaaa aggagcgcgc
    14461 aatgaagttc tggcgacccg gtattaccgg caaactgttt ctggcgattt tcgccacctg
    14521 cattgtcttg ctgatcagta tgcactgggc ggtgcgtatc agttttgagc gtggctttat
    14581 tgattacatc aaacacggca atgaacagcg attacagctg ttaagtgatg cgcttggcga
    14641 gcagtatgcg cagcatggta actggcgctt cctgcgcaac aacgatcgct ttgtctttca
    14701 gatcctgcgt tcatttgaac acgataattc ggaagataaa cccggcccgg gtatgccgcc
    14761 gcacggctgg cgtacccagt tctgggtggt cgatcaaaac aacaaagtac tggttggtcc
    14821 gcgagcgccg attccacctg acggtacacg gcgacccatt ctggtcaacg gtgcggaagt
    14881 gggcgcggtg attgcctccc ctgttgagcg gctgacccgc aatactgata tcaatttcga
    14941 taaacaacag cggcaaacca gctggctgat cgtcgccctg gcaacgttac tcgcggcgct
    15001 cgccaccttt ctgctggcgc gcggtttgct ggcaccggta aaacgacttg tcgatggcac
    15061 gcacaaactg gcggcgggcg atttcactac ccgcgtaacg cccaccagtg aagatgaact
    15121 gggcaaactg gcgcaagact tcaaccagct cgccagcacg ctggagaaaa accagcaaat
    15181 gcggcgcgat tttatggccg atatttctca cgaactgcgt acgccattag cggtgctgcg
    15241 cggtgaactg gaagccattc aggatggcgt gcgcaaattc acgccggaga cggtggcgtc
    15301 tttacaggcg gaggtcggta cactgaccaa actggttgac gatcttcatc agttgtcgat
    15361 gtctgatgaa ggcgctctcg cctatcaaaa agcaccggta gatttgatcc cactgctgga
    15421 agtggcgggc ggcgcatttc gcgaacgatt cgccagccgt ggcctgaaac tgcaattttc
    15481 cctgccagac agtatcaccg tatttggcga tcgcgaccgt ttaatgcagt tattcaataa
    15541 cttactggaa aacagcctgc gctacactga cagcggcggc agcctgaaaa tctctgccga
    15601 gcagcacgac aaaacggtgc gcctgacctt tgccgacagt gcgccaggtg tcagtgacga
    15661 tcagctacaa aaattgtttg aacgttttta tcgcaccgaa ggttcccgca accgcgccag
    15721 cggcggttcc gggctggggc tggcgatttg cctgaacatt gttgaagccc ataatggccg
    15781 cattattgcc gcccattcgc cttttggcgg ggtaagcatt acagtagagt taccgctgga
    15841 acgggattta cagagagaag tatgaccgag ttaccaatcg acgaaaacac accgcgtatt
    15901 ttgatcgtgg aagatgaacc gaagctgggg cagttgctca ttgattatct gcgtgctgcg
    15961 agctatgcgc cgacgcttat cagccacggc gatcaggtac tggcgtatgt gcgccagaca
    16021 ccaccggatc tgatcctgtt ggatctgatg ctccctggca ccgatggcct gacattatgc
    16081 cgggaaattc gtcgtttttc cgatgttccg atcgtgatgg tgacggcaaa aatcgaagag
    16141 atcgaccgcc tgctggggct ggagattggc gccgatgact acatctgcaa gccgtacagc
    16201 ccacgggaag tggtagcgcg tgtcaaaacc attttgcgcc gttgtaaacc gcaacgcgaa
    16261 cttcagcaac aggatgccga aagcccgttg attatcgacg aaggtcgttt tcaggcttca
    16321 tggcgcggta aaatgcttga cctgacgcct gcggaatttc gtctgcttaa aacgctctct
    16381 cacgaaccag gaaaagtgtt ctcccgcgag caattgctca atcatcttta tgacgactac
    16441 cgcgtagtaa ccgaccgcac cattgacagc cacattaaaa atctgcgccg caagctggaa
    16501 tctctcgacg ccgaacagtc atttatccgc gccgtttacg gcgttggtta ccgctgggaa
    16561 gccgacgcct gccgcatcgt ttagttttag cgacattact ttgttagccg gagatggcgt
    16621 tcagacccga tccggcaaca ttattccacg catggtcagc aaactgacat tatctctccg
    16681 ccctggcagg catcagaaaa tcgctcatac tttaatcagt aaacagcacc tttagatgct
    16741 gttttcgata agcaatttca atcaaggagt cattatggct ggttggtttg aactcagtaa
    16801 gagcagtgat aatcagttcc ggtttgtgct aaaagcgggc aatggtgaga ctatcctcac
    16861 cagcgagctg tatacctcaa aagcctctgc ggaaaagggc atcgcgtcgg tgcgtagcaa
    16921 cagcccgcaa gaagagcgct atgagaaaaa aacggcaagc aacggcaaat tctatttcaa
    16981 tctgaaagcc gctaatcatc aaattatcgg ctccagccag atgtacgcca ccgcgcaatc
    17041 tcgtgaaacc ggaattgcct ccgttaaagc caatggcaca agccagacgg tgaaagacaa
    17101 tacgtaatta cgatgccggg cgtgggttgt tggcgtccgg cgctttacca gatcccccct
    17161 acagcgctac aatgcccgcc cttaaagtgg gggcctcccc taaccgcttc atcaggtgaa
    17221 gcggatctga cctgtcatca gaacgagaga attatgttta aaccggaact cctttccccg
    17281 gcgggaacgc tgaaaaatat gcgttacgct ttcgcttatg gcgcagatgc tgtttatgcg
    17341 ggccagccgc gttactccct acgtgtgcgc aacaacgaat tcaaccacga aaatcttcag
    17401 ctcggcatca atgaagccca cgcgctgggg aaaaagtttt atgtcgtggt caacattgca
    17461 ccgcacaacg ccaagctgaa aacctttatc cgtgacctga aaccggtggt ggaaatgggg
    17521 ccggatgcgc tgattatgtc cgatccaggg ctgattatgc tggtgcgtga gcacttccct
    17581 gaaatgccga tccacctctc ggtgcaggct aacgccgtga actgggcgac ggtgaaattc
    17641 tggcagcaaa tgggactgac ccgcgtgatc ctctctcgcg agctgtcgct ggaagagatt
    17701 gaagagatcc ataatcaggt gccggatatg gagatcgaga tcttcgttca cggcgcactg
    17761 tgcatggcct actccggtcg ctgcctgctc tctggctata tcaacaagcg cgacccgaac
    17821 cagggcacct gcaccaacgc ctgccgctgg gagtacaacg tccaggaagg gaaagaagat
    17881 gacgttggca acatcgtaca caagtacgag ccgattccgg tgcaaaatgt tgagccgacg
    17941 ctgggtatcg gcgcgccaac cgacaaagtg tttatgatcg aagaagccca gcgtccgggc
    18001 gagtatatga ccgcgtttga agatgagcac ggcacttaca tcatgaactc gaaagatctg
    18061 cgcgccatcg cccatgtaga acgcctgacc aaaatgggcg tgcattcgct gaaaatcgaa
    18121 ggtcgtacca aatctttcta ctattgcgca cgcaccgcac aggtttaccg caaagctatc
    18181 gatgacgccg ctgcgggaaa accgttcgat accagcctgc tggaaactct ggaaggtctg
    18241 gcgcatcgtg gctataccga aggtttcctg cgtcgtcata ctcacgacga ttatcagaac
    18301 tacgaatacg gttattcagt ttctgaccgc cagcagtttg ttggtgagtt taccggtgag
    18361 cgcaaggggg acctcgcagc ggtagcggtg aaaaataaat tctccgttgg cgacagtctt
    18421 gagctgatga cgccgcaagg caacattaac tttacccttg agcacatgga aaacgccaaa
    18481 ggcgaagcta tgccggtcgc accaggcgat ggttatactg tgtggctccc ggtcccgcag
    18541 gatcttgagc taaattacgc gctgctaatg cgtaatttct ccggggaaac cacgcgtaac
    18601 ccccacggta agtgattaat ttcgattatt tttcccggat ggaaaattct tagaaaccga
    18661 tcacatacag ctgcatttat taaggttatc atccgtttcg ctgaaaaaca taatccataa
    18721 aatgctagct gtaccaggaa ccacctcctt agcctgtgta atctccctta cacgggctta
    18781 ttttttgtac ataagctgta aagaaggatt gatccctgat aaccgtcttc cattgaccac
    18841 atcgatagaa tcccccttca tagcacaatg cctttcacgt aacggtatcg tgctcgccca
    18901 ggttccggct acgcacagcc agaacgcgca tgtttgacgc ttacaaaaaa atattctcac
    18961 tctccacatt tgaatgtcag acgagcgacg ccatgtaatc ctgcaccttc tgtcttcagg
    19021 tcaactatct gcattttttt gccctgagta acacagaaat gggctgcatc attttttact
    19081 atattttctg caccagatat tctgcccctg gctaaagaag cttcggcttc ggtgtagtat
    19141 tggttatcga gtttacgctg aatattactt ttatatgcaa gaccaaattt accgatactt
    19201 gtttcatcat tatgcacagc acaaccagat ataagaaaaa tactaattaa tgatatagca
    19261 gctatctttt tcatctcacc ttcccccatt aaataccaac gacactctct agtgtttaaa
    19321 tataataatg gcatgattat tataattgaa taggattata ataaatgttc tgtacaacat
    19381 ttcctacata agtaggaatt acggacattg aggcccttca gggtaactcc atgggggctt
    19441 taatatatta tattgaagat gccactgttt agttgaatat taggtatatg ctcttttttg
    19501 aaatttatcg gtggcagccg ttagtattcg ctgtccccat tgcaagctcc tggtggtaac
    19561 cactgaatcc tccatacttg aactgacttt ttatcctccg actttcatcc tgttctgact
    19621 ccaccttttg ttttctgctc tacactatct acagaccaat cataaaggca catacgatca
    19681 tggcagaatt tcccgccagc ttactgattc ttaatggtaa aagtactgac aatctaccct
    19741 tgcgcgaagc aattatgctg ttgcgtgagg aaggaatgac gatccatgtg cgggtcacct
    19801 gggagaaagg tgatgccgca cgatttgtag aggaggcccg gaagttgggc gtcgcaacgg
    19861 tgattgccgg tggtggcgat ggcaccatta atgaagtttc tacggcgttg attcagtgtg
    19921 agggggatga catacccgcg ctgggaattt tgccattagg aaccgccaat gattttgcca
    19981 ccagtgtagg gattcctgag gcactggata aggcgctgaa actggcaatt gccggtaatg
    20041 ccattgcgat agatatggcg caggtcaaca aacaaacctg ttttattaat atggcgacag
    20101 gcggatttgg gacgcgtatt accacagaaa cgccggaaaa attaaaagcc gcgctgggtg
    20161 gcgtctctta catcattcat ggcttaatgc gcatggatac tctgcaaccg gaccgttgtg
    20221 aaatccgcgg tgaaaacttt cactggcaag gtgacgccct ggtcattggt attggtaacg
    20281 ggcgtcaggc cggtggcggt caacaattgt gcccgaactc gttaattaac gatggcttgc
    20341 tgcaactgcg catttttacc ggcgatgaaa ttcttccggc tctcgtatca accttaaaat
    20401 ctgacgaaga taacccgaat attatcgaag gcgcttcgtc gtggtttgat atacaagccc
    20461 cacacgaaat cacttttaat cttgatggcg aaccgttgag tgggcaaaac ttccatattg
    20521 aaatacttcc ggcggcgttg cgttgtcgat taccaccaga ttgtccatta ttgcgttaat
    20581 caaactattc tctcttgaac cagggagaat ccgtaatgcc ggatgcggtg taaacacctt
    20641 atccggcata cagaacaata ctacaccgta attaatttta cccgtgtctt ctgcaatgcc
    20701 aatttatcca cctctgacac agcatcatcg gtaacaataa tgtcaatttt ttcgattggc
    20761 agtacctgat taaaaccgcg acgattaaat ttcgatgaat cgagaacggc aatcacttta
    20821 tttgcggcgg tgaccatcgc cccgctaatc gaataacctt cattaaaggt cgtaatacca
    20881 ttaaccgcat caataccatc agcaccgaca aacattaaat cggcattaat atcctgcaat
    20941 gaacgctcgg caatggaacc atgcatcgag cgcgttttat ggcggaccgt gccaccacag
    21001 acgaccagag taatgtcttt attttcggaa agggcaaacg ctgccgggag actgttggta
    21061 atcaccgtga tatttttagc ggtcattaat ccttcggcga taagcattgt ggtacttccg
    21121 ctgtcgagaa taacggtcat cccttcatgg atcattttga ctgccgcctg cgctattctg
    21181 ttttttggcg cgctggcgag ctgaaatcgc tctttaaacc cgacttcctg ggtctcggtt
    21241 tcactattac cggacattat tttcgccgca ccgccatgaa agcgcgtaac cacgcctttt
    21301 tgttcgagaa agcgcaaatc ggcacggatt gtcgcttccg aggcagcaaa tactcccgcc
    21361 agatcctgaa caagcacggt tccctgttca ttcactaatt ggatgatctt attccttcgc
    21421 tcgaatgaat tcatcgtcat ttacccatat tcaattgtgg ctagtgtaaa cgaaggggga
    21481 ccattggtga atgatctgtt ttgcacaata ccgggccagc atcacgctgg cccgcggttt
    21541 catggaatga gcaacacttt gccctgcatg gcattacgag cgatgtcacg caccgcctgg
    21601 gtgaagcttt caaagcttcc acggtgagcg attaatggct ccaggcttaa cttacgttct
    21661 gtcagcaacc ggctcgccgt ttcccactcc tgccccggcc aagggctgga atagttcatc
    21721 caactgccga taaccgtcag ctctttacgc aatattttgc caaacgttgc cgatgttaaa
    21781 tgcagatcct gatgcaacgt gcccaccagc gccagttggg catgaggacc ggcaatctct
    21841 accgccagtt cgacagtttg cggtacgcca gccgtctcga ggataagctg attaaagcgc
    21901 agttcgcgta aaacgctctg catttgcggc gcgctcattt cgctactgtt aaatgtttgc
    21961 atcgcaccga aagattttgc cagtgccagt ttttctgagc taatgtcgat cgccgtcaca
    22021 ctctttgctc ccagcgcgac agcgcactga atagccagca ggccaatggt tccggcacca
    22081 ataataataa cgtttttatt ctcacaacct tgcgctaaat gaaaagcatg caggccaacg
    22141 gtaatcggct caataaaagc cccatcctca ataggcatat ccgtgggtag agcaaagaca
    22201 ttttttcgct taacgacaat atattcagca aatccaccat cacgccgcga gccaataaaa
    22261 tcatattttg cgcactggga ataaaaccct ttcaaacact ctggacaagt aaaacagggt
    22321 aataacggca cacaggcaac cgcatcgcca gggtgtaaat catcaacacc ggatcccacc
    22381 gcatcaatat agccgctaaa ttcatggcct aacgttattg gataataatg tgcaccattt
    22441 ttaaatatcc tgggtaaatc ggaaccacat aagcccgagc tggcaatttt tacccgcacc
    22501 tcatcctgat gtttaatttc aggaatgacg ctttttgcaa cgcgcacgat accatcagta
    22561 tcattcacta ctgatttcat aaaaactcct gatt
//
LOCUS       DAZSIO010000053        22412 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZSIO010000053 DAZSIO010000000
VERSION     DAZSIO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.053"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            82..2682
                     /locus_tag="ACHX5T_001877"
     CDS             82..2682
                     /locus_tag="ACHX5T_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFR8467106.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2673..3742
                     /locus_tag="ACHX5T_001878"
                     /pseudo
     CDS             2673..3742
                     /locus_tag="ACHX5T_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            3754..4294
                     /locus_tag="ACHX5T_001879"
                     /pseudo
     CDS             3754..4294
                     /locus_tag="ACHX5T_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4302..4817
                     /locus_tag="ACHX5T_001880"
     CDS             4302..4817
                     /locus_tag="ACHX5T_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8467107.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4783..5520
                     /locus_tag="ACHX5T_001881"
     CDS             4783..5520
                     /locus_tag="ACHX5T_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR8467108.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5631..6641
                     /gene="pyrD"
                     /locus_tag="ACHX5T_001882"
     CDS             5631..6641
                     /gene="pyrD"
                     /locus_tag="ACHX5T_001882"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR8467109.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6815..7357
                     /gene="zapC"
                     /locus_tag="ACHX5T_001883"
     CDS             6815..7357
                     /gene="zapC"
                     /locus_tag="ACHX5T_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR8467110.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="ACHX5T_001884"
     CDS             complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="ACHX5T_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR8467111.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8707..10815
                     /gene="rlmKL"
                     /locus_tag="ACHX5T_001885"
     CDS             8707..10815
                     /gene="rlmKL"
                     /locus_tag="ACHX5T_001885"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR8467112.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10827..12734
                     /locus_tag="ACHX5T_001886"
     CDS             10827..12734
                     /locus_tag="ACHX5T_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8467113.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12864..14117
                     /gene="pqiA"
                     /locus_tag="ACHX5T_001887"
     CDS             12864..14117
                     /gene="pqiA"
                     /locus_tag="ACHX5T_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR8467114.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14122..15762
                     /gene="pqiB"
                     /locus_tag="ACHX5T_001888"
     CDS             14122..15762
                     /gene="pqiB"
                     /locus_tag="ACHX5T_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR8467115.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15759..16322
                     /gene="pqiC"
                     /locus_tag="ACHX5T_001889"
     CDS             15759..16322
                     /gene="pqiC"
                     /locus_tag="ACHX5T_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR8467116.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16578..16745
                     /gene="rmf"
                     /locus_tag="ACHX5T_001890"
     CDS             16578..16745
                     /gene="rmf"
                     /locus_tag="ACHX5T_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR8467117.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="ACHX5T_001891"
     CDS             complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="ACHX5T_001891"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR8467118.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17402..19162)
                     /locus_tag="ACHX5T_001892"
     CDS             complement(17402..19162)
                     /locus_tag="ACHX5T_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR8467119.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19347..19799
                     /gene="matP"
                     /locus_tag="ACHX5T_001893"
     CDS             19347..19799
                     /gene="matP"
                     /locus_tag="ACHX5T_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR8467120.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="ACHX5T_001894"
     CDS             complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="ACHX5T_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR8467121.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="ACHX5T_001895"
     CDS             complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="ACHX5T_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR8467122.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22005..>22412
                     /gene="sxy"
                     /locus_tag="ACHX5T_001896"
     CDS             22005..>22412
                     /gene="sxy"
                     /locus_tag="ACHX5T_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFR8467123.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
BASE COUNT         5721 a         5258 c         5873 g         5560 t
ORIGIN      
        1 tatcgaacca tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc
       61 gtcttcagtt aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct
      121 ggtggagtgc catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca
      181 caagctgaaa cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat
      241 ttgtctcgtt tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt
      301 ctgaatcaga caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag
      361 gggattgccg cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg
      421 tttccggctt ttaaacaact ggacaaacaa gtgtgtgtgc cattggcgga gattattccg
      481 gatgccagcg taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc
      541 gccatcaaaa gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac
      601 gcgctattac tgggatattc gtttagcggg gctaacagta ttcatagcag cgcagacagt
      661 gattctggcg acagctattt tctgaattta aacagtggcg ttaatttagg cccatggaga
      721 ttacgcaaca attcaacatg gagtcgcagt agtggccaaa ccgcagaatg gaagaatctc
      781 agcagctatt tgcagcgggc ggttattcca ctaaaaggcg aactgaccgt aggtgatgat
      841 tatactgcag gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat
      901 gacaacatgc tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa
      961 agcaatgccc agataacgat taagcaaaat ggttacacca tttaccaaac ttatgtatcg
     1021 cctggtgctt ttgaaattag tgatctctat tccacgtcgt cgagcggtga tttgttagtt
     1081 gaaatcaaag aagcggacgg cagcgtcaat agctacagcg taccgttttc cagcgtgcca
     1141 ttactccagc gtcaggggcg aatcaaatac gcggtgacac tggcgaaata cagaaccaat
     1201 agtaatgaac agcaggagag caaatttgcc cagaccacgt tgcagtgggg cggaccgtgg
     1261 ggaacgacat ggtatggtgg tggacaatat gctgaatatt accgtgccgc catgtttggt
     1321 ctgggattta accttggcga tttcggagca atttcgttcg atgcgaccca ggcgaagagt
     1381 acgctggcag accaaagcga acataaaggt cagtcatatc gttttctgta tgccaaaacg
     1441 ctcaaccaat tgggcactaa ttttcaattg atgggctatc gctattcgac gtcgggtttc
     1501 tacacccttt ccgacaccat gtataaacat atggatggct acgaatttaa tgacggtgat
     1561 gatgaagata cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa
     1621 ctgcaggtca atatctccca gcaattaggc gagtacggtt cgttttattt aagtggtagc
     1681 cagcaaactt actggcatac cgatcaacag gatcggctat tacagtttgg ctacaacacg
     1741 caaattaaag atctctcgct gggggtttcc tggaactaca gtaagtcccg tggtcaacct
     1801 gatgctgatc aggtgtttgc actaaatttt tccctgccgc tcaatctgtt gctccccaga
     1861 agtaatgata gctataccag gaaaaaaaat tacgcctgga tgacctctaa caccagtatc
     1921 gataacgaag ggcacattac acaaaacctg ggtttaacgg agacactact cgatgacggt
     1981 aacctgagct acagcgtgca acagggatat aacagcgagg ggaaaacggc taatggtagc
     2041 gccagcatgg actacaaagg ggcgtttgca gatgcccgag tgggctacaa ctacagcgat
     2101 aacggcagtc aacaacaact gaactacgct ctttcaggca gtttagttgc ccattcacag
     2161 ggcattaccc tggggcaatc gctgggggaa actaacgtgc tgattgcagc accaggcgca
     2221 gagaatactc gtgtggcgaa cagcaccggg ctgaaaactg actggcgcgg atataccgtt
     2281 gtcccctatg ccacttctta tcgggaaaat cgaatcgcac ttgatgcggc atcgttaaaa
     2341 cgtaacgtgg atcttgaaaa tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt
     2401 ctggcggagt tcaatgccca tgcgggagca agggtattaa tgaaaacatc aaagcagggt
     2461 ataccgctgc gttttggcgc gatagcgacg ctggacggca tacagactaa tagcggcatt
     2521 attgatgatg atggctcgct ctatatgtcc ggtttgcccg ctcagggagc gataactgta
     2581 cgctggggcg aagctcccga tcaaatttgt catatcagtt accagcttac cgaacaacaa
     2641 attaactctg cgattacgcg aatggatgcc atatgcagat aatctttgga gaaaaatgcg
     2701 tgttattact acgactattt tttgccgccg tcttaatgct atggtgcgct caaactgctg
     2761 cttatatcgg gcagtgtcat accactcagg ggaatccgta tattggcgtc aattttggcg
     2821 ttaaaactct ggatgaagaa gaaaatacgg ctggagtagt taaagacaaa ttttatcagt
     2881 ggaacgaatc gaatgattat tatgtttcct gtgattgcga taaagacaat gtcagaagtg
     2941 gccgatgggc attcgccgcg gattcaccgt tagtctattt aggcgacaac tggtacaaaa
     3001 ttaatgacta tcttgccgcc aaagttttat tgcaggttaa aggcagttct cctactgcgg
     3061 ttcctttcga aaacgtgggc acaggggcag atacacgatg gcatatttgc gatcccggcg
     3121 gtcaacgttt aggtggccag ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat
     3181 tgcagccgtt cgttggttcg gtcgtcattc ctcctatggc gctggcgcga ttatttgaat
     3241 gctacaacat acccgcaggt gattcctgca cgactactgg tacaccggtt ttagtgtatt
     3301 acctgtctgg tacgatcaat tcacttggct catgttccgt caatgccgga gaaacaatcg
     3361 aggttgatct gggcgacgta tttgcggcta actttcgtgt tgtagggcat aagcctcttg
     3421 gggccagaac ggcagaactt gcaattccgg tcaggtgtaa cacgggaaac gcagggttag
     3481 ttaacgtcaa tctgagtctg acggcaacct cagaccccag ctatccccag gcgattaaga
     3541 cgtcacgtcc tggcgtgggc gtggtggtga ccgatagcca gcacaacatt atttcccctg
     3601 ctggtgggac actaccgctc tctattcctg atgatgcaga cagtatccgc gaatgaatgt
     3661 ctatccagtc agcacgacaa gcgtaccacc agaaaccggg cgatttgaag ccacggcaac
     3721 ggtgagaata aattttgatt aacgcgtgaa cgtatgaaga aaaaaacgat atatcagtgc
     3781 gttattttgt tctttagcct tcttaacatc catgtcggga tggctgggcc tgaacaagtc
     3841 agtatgcata tttatgggaa tgtggtcgat cagggctgtg atgtcgccac caaaagtgca
     3901 ttacaaaata ttcatattgg tgattttaat atcagtgatt ttcaggccgc gaataccgta
     3961 agcactgctg ctgatttgaa tattgatatc accggttgtg ccgctggtat tactggcgcg
     4021 gacgtccttt ttagcggcga ggctgacacc cttgcgccga cactgctcaa actaactgac
     4081 acaggcggaa gcggtggtat ggcaacgggg attgccgtgc attcttgatg cgcaaagtca
     4141 gcaagaaatc ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac
     4201 actcaaatat caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac
     4261 ggcggttctc tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct
     4321 ggattctgtt cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacgg
     4381 tcaacattag cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa
     4441 tcaatgtgca gttaggcgat gtctcgctga cacgtttttt tcatgggaat tatggcccgg
     4501 agaaatcttt catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgatgtca
     4561 ccttttcagg aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg
     4621 atgcagccag tgggctggca attgcgattc tggatgatgc gaagatattg attccgctta
     4681 accaggcgag taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc
     4741 aattgcgacc tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg
     4801 tacttcatta tgactaatac gtggaacaga ttagcgctct tgattttcgc cgttttatcg
     4861 ctgctggtgg cgggtgaatt gcaggcagga gtggtggtcg gcggaacgcg atttatcttt
     4921 ccggcagaca gagaatcgat atctatttta ctgactaata ccagtcagga atcttggctt
     4981 attaacagca aaatcaatcg cccaacgcgt tgggccgggg gggaagcgtc gacagtgcca
     5041 gcactattac tggccgctcc gccacttatt ctcctgaagc ccggtactac aggcacgttg
     5101 cgcttgctga gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta
     5161 agcattgcca gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc
     5221 tcggtattta aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat
     5281 caacaattac gctggacacg gaattcgcag ggtgtacaac tcactaaccc aacgccttac
     5341 tacattaacc tgattcaggt gagcgtaaat ggtaaagcgt taagtaatgc gggagtcgtg
     5401 ccgcctaaaa gccagcgtca gacaaactgg tgtcaggtaa tagcaccgtg tcatgtcgcc
     5461 tggcggacga taaatgatta cggcgggttg agtgcaaaga aggagcaaaa tctgccctga
     5521 aacaggttct gaaaacgttt gcgttttttt tgccgcaggt caattccctt ttggtccgaa
     5581 ctcgcacata atacgccccc ggtttgcaca ccgggaatcc aggagagttc atgtactacc
     5641 ccttcgttcg taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc
     5701 agcaattacg ccgcattacc ggaacaccgt ttgaagcact ggtgcggcag aaagtgcctg
     5761 cgaaacctgt taactgcatg ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc
     5821 ttgataaaga cggggagtgc attgatgcgt taggcgcgat gggatttgga tcgatcgaga
     5881 tcggtaccgt cacgccacgt ccacagcctg gtaatgacaa gccgcgtctc tttcgtctgg
     5941 tagatgccga aggtttgatc aaccgtatgg gctttaataa tcttggcgtt gataacctcg
     6001 tagagaacgt aaaaaaggcc cattatgacg gcgtcttggg tattaatatc ggcaaaaata
     6061 aagatacgcc agtggagcag ggcaaagatg actatctgat ttgtatggaa aaaatctatg
     6121 cctatgcggg atatatcgcc atcaatattt catcgccgaa taccccagga ttacgcacgc
     6181 tgcaatatgg tgaagcgctg gatgatctct taaccgcgat taaaaataag caaaatgatt
     6241 tgcaagcgat gcaccataaa tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg
     6301 aagacgaatt gatccaggtt gccgatagtt tagttcgcca taatattgat ggcgttattg
     6361 caaccaatac cactctcgat cgttctctgg ttcagggaat gaaaaattgc gatcaaaccg
     6421 gtggcttaag tggtcgtccg cttcagttaa aaagcacaga aattattcgc cgcttgtcac
     6481 tagaattaaa cggtcgctta ccgatcatcg gtgttggcgg catcgactcg gttatcgctg
     6541 cgcgtgaaaa gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta
     6601 aaggtccgcc gctgattaaa gaaatcgtta cccatatcta attatttctt cgaattcctc
     6661 tttataagat aatcagggct ttattttcag acctggttgt tttatattca tcgttgttgc
     6721 ttatttagac attttgtact tttattgttg aggttattaa gcgaagcgac aatggattgt
     6781 gttgctgcgg ttttataggg taggggagag gcagatgcga attaaaccag acgataactg
     6841 gcgttggtat tacgatgaag agcacgatcg aatgatgctc gatttagcca atggtatgct
     6901 atttcgctca cgttttgcgc gcaaaatgtt gactccagat gccttttctc ccgcaggctt
     6961 ttgtgttgat gatgccgcgc tctatttctc ttttgaagaa aagtgccgcg attttaattt
     7021 atctaaagac caaaaagccg aactggtgct gaatgcactg gtagcaattc gttatctcaa
     7081 accgcaaatg ccgaaaagct ggcattttgt ctcccatggt gaaatgtggg tgcccatgcc
     7141 gggcgatgcg gcctgcgttt ggttgagcga tactcacgag caggtcaatt tgctggtcgt
     7201 tgaatctggc gaaaatgccg cgctatgcct gctggcacaa ccctgcgttg ttattgcggg
     7261 tcgcgcgatg cagttgggcg acgccattaa aatcatgaac gacaggctga aaccgcaggt
     7321 gaatgttgac agcttcagcc tcgaacaggc agtctaacgc gtcagcttaa gggcagtctt
     7381 cggtacacag ctacagcaaa gaatggtgcc atcatcgccc attgctgatt ttttcagcgg
     7441 tgtgacttcg ccttctaaaa gctgaacacg gcaacttcca caaatgcccg cgcggcaaga
     7501 ataagggata cgaattccct gattttctaa ttgctccagc aacacttgtt ggttatttcc
     7561 acgaaatgcc tgtccctgcc agtcaatatc tacatttgcg tccggttgtt gcgtgatgtt
     7621 ggcggtatca tcagcggcag ctgcgccgta aatttttgcc ggagccgttg ccagaatttc
     7681 cacctcatcg ccaacgcgaa tcacgccgct attacgggca attaaattct gaccaaaatc
     7741 gacatcgccg ttatcctggg cagtgcggaa agattgcaat gtttttaatg gttcgcctgc
     7801 cggatgtttt tgcccttttt ctgggctgac ggtggtgaaa atacagcggc tacaaggttt
     7861 aaccacatca aacaccacat caccaatgcg aatcactttc cagctatctt cttcccaggc
     7921 tgacgcgccg gaaaccacca gattggggcg gaattgctcc atttttacac tggccggaca
     7981 acgttgttgg agatcacgta acgaggcttc gttagcaaga aggtaaggat agccatcagc
     8041 aaatgacaga ggtacagtgt tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca
     8101 gcgtaattgc acttcgcggg agaaaaatcc acttagccat ttgttgatcg cgtctggcgc
     8161 aattcgcgcg gtaaaatgtg tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc
     8221 agcaaaacga acatatgcgc tactgccatc tggtgcggtg agatgcaagc catcatgcac
     8281 aggtgaagga gtaaaccgta ccatctgggg aaactggcga gcggtaataa acgtaccgtc
     8341 aggttccgtg atcataaaga tgcgatcgaa ggccagacca ctgacatcag ccagagcatg
     8401 tgtaagacca atgccgcgca tcgatttaac aggatgaata aaaagccggg ttaatgtcgc
     8461 cacggtgcgg tcctcaaatg aaaataagcc ctcaacttta tgacatgacg cgcttattag
     8521 ctataatgcg caacaatttt cttagcgcct gtcccgttag gctattttat ttgtcagttt
     8581 ggccctgggt tgtgctcgaa atcctcacgt acttgtgtac gctccggttt ctccgcgctc
     8641 tccatgtcca gactgactgc aacaattacg cctactgcgg taggctctta gagtaaaagt
     8701 gacgatatga attctctgtt tgccagtacg gcccgtgggc tggaagagct gttaaaaact
     8761 gaactggaaa atctgggggc cgttgaatgc caggtggttc agggtggggt ccatttcaag
     8821 ggcgacacac ggcttgttta ccagagcctg atgtggagcc gcctggcctc gcgtattatg
     8881 ttgccgctgg gcgagtgtaa ggtttacagc gatttagacc tctatctcgg tgttcaggcg
     8941 atcaactgga cagagatgtt taatcctggc gcgaccttcg ctgtccactt cagtggtttg
     9001 aatgacacca tccgcaacag tcagtacggc gcgatgaaag tgaaagacgc gatcgtcgat
     9061 gctttcacac ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc
     9121 gttaacgtct ggctgcataa agaaaccgcc agtatcgctc ttgatctgag tggtgatggt
     9181 ttacatctgc gtggctatcg cgatcgtgct ggtattgcgc cgatcaaaga aaccctggca
     9241 gccgctattg tgatgcgatc cggctggcag tcaggaacac cgctgctcga tccgatgtgt
     9301 ggttccggta cgttgctgat tgaagcggcg atgctggcga ccgatcgcgc accaggcttg
     9361 caccgtgggc gttggggctt tagcggctgg gcgcagcatg atgaagctat ctggcaggaa
     9421 gtgaaagcgg aagcgcaaac tcgcgcccgt aaaggcctag ctgagtatag ctctcatttt
     9481 tacggttcgg acagcgacgc acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg
     9541 gggattggtg aactgatcac ctttgaggtg aaagatgtcg cgcaactgac caatccgctg
     9601 ccgaaagggc cgtacggtac agtgttgagc aacccgccat acggtgaacg tctggacagc
     9661 gaaccggcgc tgattgcgct gcatagtctg ctgggccgaa tcatgaaaaa ccagttcggt
     9721 ggctggaatc tctctttgtt tagtgcctcg ccggatctgc taagctgcct gcagctgcgt
     9781 gcagacaaac agtacaaggc gaaaaacggc ccgctggact gcgtacagaa aaattaccac
     9841 gttgccgaaa gcacaccaga cagtaaaccg gcaatggtag cggaagatta cgccaaccgc
     9901 ctgcgtaaga acctcaaaaa attcgagaag tgggcacgcc aggaagggat tgaatgttac
     9961 cgcctgtatg acgccgatct gccggaatat aacgttgccg ttgaccgtta tgccgactgg
    10021 gtggtggtgc aggagtatgc gccgccaaaa actattgatg cgcacaaagc gcgtcagcgt
    10081 ctgttcgata ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa caaactggtg
    10141 ctgaaaaccc gtgaacgcca taagggcaaa aaccagtacc agaaactggg cgagaagggc
    10201 gagtttcttg aagttaccga atataacgct cacttgtggg tgaacctgac ggattatctc
    10261 gatactggtc tgttcctcga tcaccgtatc gcccgccgta tgctcggtca gatgagcaaa
    10321 ggcaaagatt tcctcaacct gttttcttat accggcagcg ccaccgtgca tgcgggatta
    10381 ggcggtgcac gcagcaccac caccgtggat atgtcgcgta cttatctgga atgggcagaa
    10441 cgcaacctgc gtctgaatgg cctgactagg cgtgcgcatc gcctgattca ggccgattgc
    10501 ctggcgtggc tgcgtgaggc aaatgaacag ttcgatctga tctttatcga tccgccaacc
    10561 ttctctaact caaaacgaat ggaagatgcg tttgatgttc agcgcgatca tctggcgctg
    10621 atgaaagatt tgaaacgtct gctgcgtgca ggtgggacga tcatgttctc gaacaacaaa
    10681 cgtggcttcc gtatggatct cgacggcctg gcaaaactgg gactgaaagc acaagaaatt
    10741 acgcaaaaaa cgctctccca ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg
    10801 attaccgcag cctgaaagga atagtaatgt cattaatcag tatgcatggc gcatggctgt
    10861 cgttcagcga cgcgccgctt ctcgataacg cagaactgca tatcgaagat aacgaacgtg
    10921 tttgtctggt gggccgcaac ggcgcaggaa aatcgacgtt aatgaaaatc ctcaaccgtg
    10981 aacaagggct ggatgacggt cgcattattt atgagcaaga tttgattgta gctcgtctgc
    11041 aacaggatcc gccgcgtaac gttgagggta gcgtttatga tttcgttgcc gaaggcattg
    11101 aagaacaagc ggaatatctg aaacgctatc acgatatttc gcgcctggtg atgaacgacc
    11161 cgagcgagaa aaatctcaac gaactggcga aggttcagga acagctggat caccacaacc
    11221 tgtggcagct ggaaaaccgc atcaacgaag tgctggcgca actggggtta gatcctaacg
    11281 ttgcgctgtc gtcgctttcc ggcggctggt tgcgtaaagc ggcattagga cgcgcgctgg
    11341 tgagcaatcc gcgcgtattg ctgctggacg aaccgactaa ccatctggat atcgaaacca
    11401 tcgactggct ggaggggttc cttaaaacct tcaatgggac gattattttc atctcccacg
    11461 accgttcgtt tatccgcaat atggcgacgc gcattgttga tctcgatcgc ggcaagctgg
    11521 tgacctatcc agggaattac gaccagtacc tgcaggaaaa agaagaagcc ctgcgcgtgg
    11581 aagaactaca aaatgccgag ttcgatctca aactggcgca ggaagaagtg tggattcgcc
    11641 aggggatcaa agcacgccgt acccgtaatg aaggccgcgt acgcgccctg aaagcgatgc
    11701 gtcgcgaacg tggtgaacgt cgcgaagtga tgggtaccgc aaagatgcag gtggaagagg
    11761 ccagccgctc cggtaagatc gttttcgaaa tggaagacgt ttgctaccag gttaacggta
    11821 agcaactggt gaaagatttt tctgcccagg ttctacgtgg cgacaaaatt gccctgattg
    11881 gtccgaatgg gtgcggcaaa accacgctgc tgaaactgat gctcggtcag cttcaagcgg
    11941 acagcgggcg tattcacgtt ggcaccaaac tggaagtggc ttatttcgat cagcaccgcg
    12001 cggaactgga tcccgataaa acggtgatgg ataaccttgc cgaaggtaag caagaggtga
    12061 tggttaatgg caagccacgc cacgtattgg gctatttgca ggactttctg ttccatccga
    12121 aacgggcgat gacgccagta cgtgcgtttt ctggcggtga gcggaaccgc ttgctgctgg
    12181 cgcgtttgtt cctcaaacca agcaacttat tgattcttga cgaaccgacc aacgatcttg
    12241 atgtcgaaac gctggaactg ctggaagaac tgatcgacag ctatcagggc acggtattgc
    12301 tggttagcca cgatcgtcag tttgtcgata acaccgttac agaatgctgg atcttcgaag
    12361 gcggcggtaa aattggtcgt tatgttggcg gttatcatga tgcccgtggt cagcaagaac
    12421 aatatgtggc actcaaacag cctgcggtga aaaaaagcga agaagccgcc gcgccaaaag
    12481 cagaaactgt aaaacgcagc agtagcaaac taagctataa attgcagcgc gaactggagc
    12541 agctaccgca attgctcgaa gatctggagg cgaagctgga agccctacag acgcaagtgg
    12601 cggatgcttc cttcttcagt cagccgcatg agcagacgca aaaagtgctt gctgatatgg
    12661 ctgctgcaga gcaggagctg gagcaagcct ttgaacgctg ggagtatctt gaagcgttaa
    12721 aaaatggtgg ctgatcgcaa aatagtcgat ttaaacagag acagctgaca acggtaaaat
    12781 tgcctgatga actgggttta tcaggcatga aaaccaacac tttgttagtc atctgtaaaa
    12841 tagcgcatca ttaaggagta ccaatgtgcg aacatcatca tgccgcgaag cacatcctgt
    12901 gctcgcagtg tgacatgctg gtggcgttac cgcgccttga gcatggtcag aaagcggcat
    12961 gtccccggtg tggcacaacg ttaaccgtgg cgtgggatgc ccctcggcag cgtcccaccg
    13021 cctatgcgtt ggctgcactg ttcatgctgt tgctgtccaa cttgtttcct tttgtgaata
    13081 tgaacgttgc aggagttacc agtgaaatta cattactgga aattcccggc gtgctttttt
    13141 ctgaggacta cgccagcctc ggcacctttt tcctgttgtt tgtgcaactg gttcccgcgt
    13201 tttgtctgat aaccattctg ttactggtga atcgcgcgga attaccggtc cgtttaaaag
    13261 agcaactggc acgggtgctt tttcaactca aaacctgggg aatggcggag attttcctcg
    13321 cgggtgtgct ggtcagtttc gttaaactga tggcttacgg cagcattggg gtaggcagca
    13381 gctttctccc ctggtgttta ttttgtgtcc tgcaactgcg cgcttttcag tgcgttgatc
    13441 gtcgctggtt atgggacgac atcgccccga tgccagaact gcgccagccg ctaaaaccag
    13501 gcgtcacggg gatacgtcag gggctgcgtt cgtgctcctg ttgtacggca atccttcctg
    13561 ctgatgaacc cgtgtgcccg cgttgtagta ccaaagggta cgttcggcgt agaaacagcc
    13621 tgcagtggac actcgcgctg cttgtaacgt ccatcatgct gtatcttccg gctaatattt
    13681 tgcccatcat ggtgacggat ttattaggct cgaagatgcc gtcgacgatt ctcgctgggg
    13741 tcattctgtt atggagcgaa ggctcttatc ccgtcgctgc ggtgatcttt ctggccagta
    13801 ttatggtgcc aacgttaaag atgatcgcca tcgcgtggct gtgttgggat gccaaagggc
    13861 atggcaagcg cgacagtgaa agaatgcatt tgatttatga agttgttgag tttgtaggcc
    13921 gctggtcgat gattgacgtt ttcgttatcg cggtgctctc ggcgctggtg cgtatgggag
    13981 gtttaatgag tatttatccg gcaatgggtg cattaatgtt tgctttagtc gtcataatga
    14041 caatgttttc tgctatgacg tttgacccgc gtttgtcgtg ggatcgtcaa cctgaatcag
    14101 agcatgagga gtcctgacag tatggaatct aataatgggg aagccaaaat ccagaaagtg
    14161 aagaactggt ctcccgtgtg gatatttcct atcgtcacgg cgctcattgg ggcctgggtt
    14221 cttttttatc attacagcca tcagggaccg gaagtgaccc tgatcaccgc gaatgcggaa
    14281 ggaattgaag gtggcaaaac caccattaaa agccgtagcg ttgacgtcgg cgtggttgaa
    14341 agcgccacac tggctgatga tttgacgcac gttgaaatca aagcgcggct gaattccggt
    14401 atggaaaaat tgctgcataa agacaccgtc ttttgggtgg tgaaaccgca gattggtcgc
    14461 gaagggatta gcggcctggg aacgctgctg tctggagttt atatcgaact gcagccaggc
    14521 gcgaaaggca gcaaaatgga taaatacgat ttgctggact caccaccgtt ggccccgcct
    14581 gatgcgaaag gtatccgtgt gattctcgat agcaaaaaag ccgggcagct ctcgccagga
    14641 gatccggtgc tgttccgtgg ctatcgggta ggttcggttg aaaccagcac cttcgatacg
    14701 caaaaacgca atatcagtta tcaactgttc atcaatgcac cttatgaccg actggtgacc
    14761 agcaatgttc gcttctggaa agatagtggc attgcggttg atctgacgtc agcggggatg
    14821 cgtgtggaga tgggctcatt gacaacgctg cttagtggcg gtgttagctt tgatgtgccg
    14881 gaaggtctgg atttagggca gccagtggca ccgaaaacag ctttcgtttt gtatgatgat
    14941 cagaagagca ttcaggattc gttgtacacc gatcacattg attatctgat gttctttaaa
    15001 gattcggtac gcggtctgca accgggagct ccggtagaat tccggggtat tcgcctgggt
    15061 accgtaagca aagtgccatt ctttgcgccg aatatgcgtc agacatttaa cgatgattac
    15121 cgtattccgg tactgattcg tatcgagcca gagcggctga aaatgcagct tggcgaaaat
    15181 gcggatgttg ttgagcacct tggcgaattg ttgaaacgtg gtttacgcgg atcgctgaaa
    15241 accggaaacc tggtcactgg tgcactgtat gttgatctcg atttctatcc aaatacgcct
    15301 gcaataaccg gtattcgtga atttaatggt tatcagatta tcccgactgt tagcggcggc
    15361 ttggcgcaaa tccagcaacg actgatggaa gcgttggata agatcaacaa actgccattg
    15421 aatccgatga ttgaacaggc aaccagtacg ctttctgaaa gtcagcgcac aatgaaaaac
    15481 ctgcaaacga cgctggatag catgaacaag atcctcgcca gccagtcgat gcagcagttg
    15541 ccgacggata tgcagtcaac gttgcgtgaa ttgaatcgca gcatgcaggg cttccagcct
    15601 ggctccgcag cctacaacaa gatggtggcg gatatgcagc gccttgatca ggtgttgcga
    15661 gaactgcaac cggtgctgaa aacgctcaat gagaagagta acgcgctggt atttgaagcg
    15721 aaggacaaaa aagatccaga gccgaagagg gcgaaacaat gaaaaagtgg ctagtgacga
    15781 ttgcagcact gtggctggcc ggatgcagct ccggcgaaat taataaaaac tattaccagt
    15841 tacctgtggt gcagagcggt acacaaagta ccgccagcca gggcaatcgt ctgttatggg
    15901 tagagcaggt cactgttcct gactatctgg cggggaatgg tgtggtttat caaaccagtg
    15961 atgtgaagta tgtgattgcc aacaacaact tgtgggccag cccgttggat caacagttgc
    16021 gcaacaccct ggttgccaac ctgagtacgc aactgcccgg ctgggtggtt gcctcccagc
    16081 ctctgggaag cgcccaggac acgctcaatg ttaccgtaac ggagtttaac ggtcgctatg
    16141 atggcaaggt cattgtcagt ggtgagtggc tgttgaacca ccagggacaa ctgatcaaac
    16201 gtccgttccg tctggaagga gtgcaaactc aggatggtta cgatgagatg gttaaagtgc
    16261 tggccggtgt ctggagtcag gaagccgctt ctattgcaca agagataaag cgtctacctt
    16321 aattataaag atttgtaaat ataaccgtct ccggtatgtt gcctgaggcg gtttttttgt
    16381 ttctaacgtg cggaaaaatt tgttcctctt cacatttttt gtacaaccga catgcccgtg
    16441 tagctcacaa atatgacagt ggcgtgaatt ttgcgcattg acggcagtta tgattcgcgg
    16501 tattgcttaa ctgtgattgc acatttagta atcactgttt tcttttccac cagaaaccag
    16561 tatgagggaa acgaggcatg aagagacaaa aacgagatcg cctggaacgg gcacatcaac
    16621 gtggttatca ggccggcatc gccggacgct caaaagaaat gtgtccctat cagacgctga
    16681 gtcaaaggtc acaatggctg ggaggctggc gagaagccat ggcggacagg gtagtaatgg
    16741 cctgattctg tctctttaaa aagaaacctc cgcattgcgg aggtttcgcc ttttgatact
    16801 ctgtctgatg ataatcagaa ggcagacgta tcctggaaca gaccgacttt caggtcgctg
    16861 gcggtataga tcagacgaca atcaaccagc acttcgccat ctgccagacc cataatcaga
    16921 cgacgattaa caatgcgttt aaagtgaata cggtaggtca cttttttcgc tgtcggcagt
    16981 acctgaccag tgaatttcac ttcgccaacg cccagcgcgc ggcctttacc ttcgccgccc
    17041 agccagccga ggtagaaccc taccagctgc cacattgcgt ccaggcccag gcatcccggc
    17101 ataaccggat cgccaatgaa gtggcatccg aagaaccaca gatccggatt gatatccagt
    17161 tctgcttcaa catacccttt gtcgaagtta ccacccgttt cggtcatttt gaccacacgg
    17221 tctatcatca gcatgttcgg tgctggcaat tgcgggcctt tagcgccaaa cagttcaccg
    17281 tgaccagagg caagaaggtc ttcttttgta taggattcgc gtttatctac catgttctct
    17341 gtaagcctta ttttattgaa gcacgcagga tagctaacac gtgtacgctg aacaagtccg
    17401 atcagttcgg aataaaccag ttcagccaac gtaatggcca tggaaaacgg tgacgtcctt
    17461 cctgttgcga tgcttgcgcg atacgttcct ggatggtttg catcagcgtc gtttggcctt
    17521 cgccatccca caccagattt aataataacg gcagtgcgtc agtcacatcg tctactgccc
    17581 agatggtgaa tttgccttct tctaccgctt tcaccagttc actgtgaaga cttaaatggc
    17641 gaacgttagc agtggggata atgacacctt gtttccccgt taactcacgt tgctggcaaa
    17701 tagcaaagaa gccttcgatt ttctcattta aaccaccgac cggctggacg cggccgaact
    17761 gatcgactga acctgtgata gcgatactct gattcactgg cacatcagcg agggcgctta
    17821 tcagggcgca gagttcagcc atcgaggcac tatcgccatc cacttcactg tatgactgct
    17881 caaatgtcag cgatgctgag aaggggatct gttgctcaag ctgtagttcc gacatcagga
    17941 acgcctgcat gatcatcatc cctttcgcat ggatattgcc gccaagctcc gctttgcgtt
    18001 cgatgtcggt gaattcacca tcgccaatat gcacaacgca gctaatgcga gaaggttcgc
    18061 caaaagcgcg tggatgaccc ggaaattcaa tgaccgaaag ggcgttaatt tgcccgatgc
    18121 gttcgccttc ggtttcaatc aggatttgct cctgaaggat ctcatcctgc atacgttcag
    18181 cgaggaaacc ttcgcgccat tcacgctgct gcagcattaa gtttagctgc tcgccggaga
    18241 aggtgtcgcc atcacacagg gaggcgacct ctttacactg gcggaggatc cactgcgggc
    18301 taagcggaag tgtttcttgt tcaccggtgt agcgtgctgc ttcgcggata agtaccggcc
    18361 aagcatccgc tcccggggca ggtaagtgat tatgtctggc ggtaaatgtc acccagcgac
    18421 accactggct tactgactcc gcatcgacaa tctgcagagt atcttcaaat tcgctataaa
    18481 tagcctgctc tgaaagctct ggctccatct cctggaaatc agccaatgat tcgcgttcgc
    18541 ctaccagaat gactttcagc ttcaatggca ttgaaggcac agagacgggg agagggcgcg
    18601 actcatcaaa cgcaacccag tcaaaacgct cgcggttaac gatatttttc agccgcatcc
    18661 acagcagagg ttgcgccagc agtgtacgca aagagatgat gagaataccg ccatttgcct
    18721 gatgcaccag accaggctgc agggtaatgt cgccattaaa ctggcgcagg cagccaaaga
    18781 gttgctccgc ttctacccag tcggcagcga caacttgcgt taaagtcgca aaattatcat
    18841 ctgcactcac tgcgtgacgt aagcggatgg agtggccgga aacctcatag tgatcgccca
    18901 ccagttggcc tgcatcgctt tgtagcgtac gcgcggcatc ggcaataaga ttcagatact
    18961 cagactcttc cggggcctta gccagcataa aagaggagga tgctcgcgta tgcagcaact
    19021 gctccagcgc aaattgcagt cgcggttgag tatcactgaa taaaggatcg ttttcgtcaa
    19081 tcaaatgtgg ctgagcaaat atttcctgat agctatcggt atcaggaacc aggtcacgcc
    19141 atgcaagttt cgtaatggtc aaagttgatg ttttttagtc tgttgtcaaa gccgcgatta
    19201 taccgtaacc ggcactacag cacacgtaga aagcaccgac aatactcctg gctgggcgtt
    19261 aaagctcaca ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag
    19321 agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg
    19381 gttggaaatg gaagtatctg gtgaagaagc atcgcgaagg ggagttaatt acccgttaca
    19441 tagaagccag tgccgcccag gaagccgtcg atgtgttgct ctcgctcgaa aatgaacccg
    19501 tgctggtaaa tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc
    19561 agaccattcg ggccagacgg aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa
    19621 aatcgatcga tctggaattt attgtctggc aacgtctggc tggtcttgca cagcgtcgcg
    19681 gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat
    19741 acgcgaataa aatgtcttct cttaagcagg atctgcaggc attgctgggt aaggaataac
    19801 tgacgaaagt cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc
    19861 taccagacga taacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg
    19921 atctctacgc gacgatccgg agccaagcag tcgatcagtg cagcacgctg tttcacgttg
    19981 tcacaggtgt tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct
    20041 gccgggatac ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga
    20101 ccctggttgt aagcgtcaga accgatgcgg tcagtgtaac ccagaacaac tacggaaccg
    20161 tctttcggat ctaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc
    20221 ggtttcaggg ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg
    20281 gtctgtactt ccggtgccgg agctggagca actaccggag ctgcttcgcc ctgaccgaaa
    20341 cggtaggaaa cacccaggct cagcagaccg ttgtccggac gagtaccgat ggtgtttgcg
    20401 tcaccgatgt tgttggtcca ctggtattcc agacgggtag cgatttcagg agtgatcgcg
    20461 tactcaacac cacctgcgaa aaccggagaa acgccagtgt cgtggtcttt aaaggatgcg
    20521 ccaccaggta cgttagcctt ggtgtctgca cgccatacca taccacccag acgagtgtag
    20581 atgtccagat cgtcagtgat tgggtaaccc agtttagcgg tcagctgaac gccctgagct
    20641 ttgtatgcgc cgttgatgtt gtcgcctttg tacggcatac gacctaacca gtcgtaaccc
    20701 atttcaaaac caacatacgg gttaacctgg taaccaccaa aagcacctgc acccagttgg
    20761 ttttcgtggg tcggaccatt gttaggaata aaaccagtgt catggtactg ggaccagccc
    20821 agtttagcac cagtgtacca ggtgttatct ttcggagcgg cctgcgctac ggtagcgaaa
    20881 ccagccagtg ccactgcaat cgcgatagct gtctttttca ttttttgcgc ctcgttatca
    20941 tccaaaatac gccatgaata tctccaacga gataacacgg ttaaatcctt caccggggga
    21001 tctgctcaat attaactcta ccgatatctt cggcttatgc cgagcacccc tggcgatgta
    21061 aagtctacaa cgtagttgaa aacttacaag tgtgaactcc gtcaggcata tgaaaaaaaa
    21121 gtcttgtata aggtatgttt aatctttttg tcagcgacaa tttacagaaa aaaaacgcgg
    21181 aaaccgcttc agacaagcct tcgcaaggaa aattagtcac gactgaaagc attggctggg
    21241 cgacaaaaaa agttctagga ttaatcctaa atttacttaa tgatacaaat tagagtgaat
    21301 ttttagcccg gaaagttgtc tcgttgtgtg agaggatgcg cttaccggac gcataataaa
    21361 ccccatagcg ttaccttcat ttgccgcatc aacaagttca gcatgctctt cttcagtcaa
    21421 atcatctgtc aaccaaccga tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat
    21481 tgactccact gtgtggcaag gggagagctg gctaatctgc attactttcg ttaagggtag
    21541 cccagatgcc tgaacccatt cccgactcag tttttgttgc ggtgttaacc agagttgcca
    21601 gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg tcatcatggg
    21661 ctgatcttcg cgatagacaa cttcactgat aagcccggct gtagtgtttt ccgtagagac
    21721 acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt
    21781 gtacataatc aatccagccc ctgtgagtta ctgtatggat gtgcagtaca tccagtgaca
    21841 acaaagatca accctatttt cggaaagagc ctcgcaaatt ttgtcgttgg tgacgggaaa
    21901 acataaatta atcttgcccc ttaagaataa gttgcctatt ttcgtagtta acggatccgt
    21961 taatgtgaat cattctttta tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct
    22021 ataagcggat ctataaatca caagaatacc tggcaacgtt gggcacaatt gaataccgat
    22081 cattgtttgg cagttacagc ctgaccgttg acgacacggt gtttgcgatg gtttctgatg
    22141 gtgagttgta tcttcgggct tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg
    22201 tctggctgac atataaaaag tgtggccgat ccgttaccct caattactat cgggttgatg
    22261 aaagtctatg gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag
    22321 cgctgaaaga gaaaagcacg cgcaataccc gggaaagact gaaagatttg cccaatatgt
    22381 cttttcatct ggaagcgatt ctcggggagg tg
//
LOCUS       DAZSIO010000054        22191 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZSIO010000054 DAZSIO010000000
VERSION     DAZSIO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22191
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.054"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(622..1713)
                     /gene="ychF"
                     /locus_tag="ACHX5T_001897"
     CDS             complement(622..1713)
                     /gene="ychF"
                     /locus_tag="ACHX5T_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR8467124.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1830..2414)
                     /gene="pth"
                     /locus_tag="ACHX5T_001898"
     CDS             complement(1830..2414)
                     /gene="pth"
                     /locus_tag="ACHX5T_001898"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR8467125.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2692..2970
                     /gene="ychH"
                     /locus_tag="ACHX5T_001899"
     CDS             2692..2970
                     /gene="ychH"
                     /locus_tag="ACHX5T_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR8467126.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(3025..4704)
                     /gene="dauA"
                     /locus_tag="ACHX5T_001900"
     CDS             complement(3025..4704)
                     /gene="dauA"
                     /locus_tag="ACHX5T_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR8467127.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4829..5776)
                     /gene="prs"
                     /locus_tag="ACHX5T_001901"
     CDS             complement(4829..5776)
                     /gene="prs"
                     /locus_tag="ACHX5T_001901"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR8467128.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5927..6778)
                     /gene="ispE"
                     /locus_tag="ACHX5T_001902"
     CDS             complement(5927..6778)
                     /gene="ispE"
                     /locus_tag="ACHX5T_001902"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR8467129.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6778..7401)
                     /gene="lolB"
                     /locus_tag="ACHX5T_001903"
     CDS             complement(6778..7401)
                     /gene="lolB"
                     /locus_tag="ACHX5T_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR8467130.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7615..8871
                     /gene="hemA"
                     /locus_tag="ACHX5T_001904"
     CDS             7615..8871
                     /gene="hemA"
                     /locus_tag="ACHX5T_001904"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR8467131.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8913..9995
                     /gene="prfA"
                     /locus_tag="ACHX5T_001905"
     CDS             8913..9995
                     /gene="prfA"
                     /locus_tag="ACHX5T_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR8467132.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9995..10828
                     /gene="prmC"
                     /locus_tag="ACHX5T_001906"
     CDS             9995..10828
                     /gene="prmC"
                     /locus_tag="ACHX5T_001906"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR8467133.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10825..11217
                     /gene="sirB2"
                     /locus_tag="ACHX5T_001907"
     CDS             10825..11217
                     /gene="sirB2"
                     /locus_tag="ACHX5T_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR8467134.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11221..12030
                     /gene="sirB1"
                     /locus_tag="ACHX5T_001908"
     CDS             11221..12030
                     /gene="sirB1"
                     /locus_tag="ACHX5T_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFR8467135.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            12066..12920
                     /gene="kdsA"
                     /locus_tag="ACHX5T_001909"
     CDS             12066..12920
                     /gene="kdsA"
                     /locus_tag="ACHX5T_001909"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR8467136.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(13069..13176)
                     /locus_tag="ACHX5T_001910"
     CDS             complement(13069..13176)
                     /locus_tag="ACHX5T_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR8467137.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(13581..14681)
                     /gene="chaA"
                     /locus_tag="ACHX5T_001911"
     CDS             complement(13581..14681)
                     /gene="chaA"
                     /locus_tag="ACHX5T_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR8467138.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            14951..15181
                     /gene="chaB"
                     /locus_tag="ACHX5T_001912"
     CDS             14951..15181
                     /gene="chaB"
                     /locus_tag="ACHX5T_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR8467139.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            15339..16034
                     /gene="chaC"
                     /locus_tag="ACHX5T_001913"
     CDS             15339..16034
                     /gene="chaC"
                     /locus_tag="ACHX5T_001913"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR8467140.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(16078..16431)
                     /gene="ychN"
                     /locus_tag="ACHX5T_001914"
     CDS             complement(16078..16431)
                     /gene="ychN"
                     /locus_tag="ACHX5T_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR8467141.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            16616..18010
                     /gene="ychO"
                     /locus_tag="ACHX5T_001915"
                     /gene_synonym="iatA"
     CDS             16616..18010
                     /gene="ychO"
                     /locus_tag="ACHX5T_001915"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR8467142.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(18011..18661)
                     /gene="narL"
                     /locus_tag="ACHX5T_001916"
     CDS             complement(18011..18661)
                     /gene="narL"
                     /locus_tag="ACHX5T_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR8467143.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18654..20450)
                     /gene="narX"
                     /locus_tag="ACHX5T_001917"
     CDS             complement(18654..20450)
                     /gene="narX"
                     /locus_tag="ACHX5T_001917"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR8467144.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            20789..22180
                     /gene="narK"
                     /locus_tag="ACHX5T_001918"
     CDS             20789..22180
                     /gene="narK"
                     /locus_tag="ACHX5T_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR8467145.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         5505 a         5499 c         5719 g         5468 t
ORIGIN      
        1 ttgtcgttac ttattccaat tcgttactat gaatatggca aaaatattac aacaaacagc
       61 ggttaaaaaa tttggtgaag attttttgta ataatcctaa ttagttatgg gattttcgct
      121 tgatgtatct gcataacaaa aacaatatgc gccatttttg cattactaat aagaaaaagc
      181 aaaaaacatc gatattcatt tttgttcaat ggattttctg gcctatcgtt attattgtgc
      241 tactggttat cattttcatc ttgccaggat cgcacgtaat cacgttattc ggggacttta
      301 tctgcgggat attacccttc aggtaagtcc ttttctcgcc agcatattgg agcgagatca
      361 atattgatca gctgtgaaga gggctgatgt gtagtggaac aaaaactgac gttatatcac
      421 tgctaatgag atatcagtca tctgaacact tatgtcttta attattctcg tggttcactg
      481 taggcaataa gcacaaaagt gtaggatgtt acaagaatga ttaggactcg gtgaaatgaa
      541 aaatccacgc aattgcgtgg attttatata cttttgcgct cttcatgaga tttagcgaaa
      601 cctcatgaga caataaatta attagacgtt gaaaaggaag ttcatcacat cgccatcttt
      661 cacgatgtaa tctttacctt ctgcacgcat tttgcctgct tctttcgcgc cttgttcacc
      721 tttgtaagtg atgaaatctt caaacgagat ggtttgtgca cggataaagc ctttttcaaa
      781 atcagtatgg attttgcccg ctgcctgcgg cgcggttgct ccaaccggaa tggtccatgc
      841 acgcacttct ttcaccccag cggtgaagta agtttgcagg ttcagcagct tataaccggc
      901 acggatcaca cggttcaggc ccggttcttc cagcccaagc tcctgcataa actcgtcacg
      961 ttcttcgtcg tccagttcgg caatgtctgc ttcaacagca gcacaaaccg gaaccacaac
     1021 agaaccttct ttcgccgcga tttcacgcac ctggtcaaga tatgggttgt tttcaaaacc
     1081 atcttcgttg acgttggcta tgtacattgt tggtttcagc gtcaggaagc tcaggtaacg
     1141 aatagccgct ttctcttcag cgcttaaatc cagcgcgcgc agcatacctg cgttttccag
     1201 ctggggcagg catttttcca ggaccgccag ctcagctttc gcgtctttat cgccaccttt
     1261 ggctttcttc tgtacgcgat gaatcgcacg ttcgcaggtg tcgaggtctg ccagcgccag
     1321 ttcggtgttg ataacttcaa tatcgtcagc cgggttaact ttgccggaaa cgtgaatgat
     1381 gttgtcattt tcaaagcagc gaacaacgtg accgatcgct tcggtttcac ggatgttggt
     1441 caggaactgg ttacccagac cttcgccttt cgatgcgcct tttaccagac cggcgatatc
     1501 gacaaattcc atggtcgtgg gaagcgtacg ttgcggtttt acgatttcag ccagttgatc
     1561 caggcgaggg tcaggcattg gtacgacgcc tgtgttcggc tcaatggtgc agaatggaaa
     1621 gttggccgct tcaataccgg ctttggtcag cgcgttgaac agggtagatt tcccgacgtt
     1681 gggcaaaccg acgataccgc atttgaatcc catgatttaa atcaccttaa tatcttaata
     1741 atcaacctgt tattgctaac agattgcaga aatggaaata actttgccta ttatacacgg
     1801 cactcggcaa aaatgccgca gacaacgact tattgcgctt taaaggcgtg caatcggttc
     1861 gttgctttgg tcaagccatc tgtaaaccac atttcagtac aacgcgccgc ttcgtcgatg
     1921 gcttcatcaa ttaacttctg ttcactaaca ggcggtttgc ctaacacaaa accgacaact
     1981 ttatttttat cgcccggatg accgattccg atgcgtaaac ggtgaaagtt agggttatta
     2041 cccaatttac tgatgatgtc tttcagtcca ttgtgaccac catggccacc gcccaactta
     2101 aatttggcga cgccaggagg cagatccagt tcgtcgtggg ccaccagaat ttcgtccgga
     2161 ttaatgcgga aaaaactggc catcgccgca acggctttgc cgctgagatt cataaatgta
     2221 gtcgggacta acaggcggac atcttcgctt ccaagggtga ctcgcgaagt ataaccaaag
     2281 aatttagcct cttcgcgcag cggagcgcgc aaacgctctg ccagtaagtc aacgaaccag
     2341 gcaccagcat tatgtcgcgt tgcggcgtat tcagcaccgg ggttcgccag gccgacaatc
     2401 aatttaatcg tcacgttttt ttgtcctgag tgtgtacata actggcgcgt agtttactgg
     2461 ttgcggcccc gcttgacaaa aaactgcgta tcaaatgcag ataacgtaat aattgcctga
     2521 gtggactatt agaaagtcaa ggtgttcagg cgtttatttg taaagttttg ttgaaataag
     2581 ggttgtaatt gtgattacgc ccgcacataa cccacagggt gttgtctata ctttacacat
     2641 aaggaagagg ggtattccct gttacaaccc agaaagttcc ggaggtgaca tatgaaacgc
     2701 aaaaacgctt cgatactcgg taacgtgctc atggggttgg gtctggtggt aatggtggtc
     2761 ggcgtggggt attcaatcct caaccagtta ccacagttta atatgcccca gtatttcgca
     2821 catggtgcag tgctaagtat tttcgtcggt gccattctct ggctggcggg tgcccgtgtt
     2881 ggcgggcatg aacaggtgtg cgaccgttac tggtgggttc gtcactatga caaacgttgc
     2941 cgccgtagcg ataatcgccg tcatagctaa caaataatgc agtttgctga ccagtcagtt
     3001 tgcgctgact ggtcaatctc gtacttataa atccgccatc gccgcgcgac gattcgggaa
     3061 gaacgcgagg cgtcccggga tcggttgaat gcctgcgcga gccatagttc gcagtggctg
     3121 gaattccacg ttgcacacgc gcagttcaca tccttcgggc agacgcttca caaaacgctg
     3181 gaacgcatca agaccaccag catcaagtac cggaacggca tcccacttca gaatcacaat
     3241 ccgtttgcct tcaagacgtg actccaggtc cgtgaataag ccttcagcag cagcaaaaaa
     3301 cagcgggcca ataacgcgca gaaccaggac atcgtctgga acatctacga ctaccggtgc
     3361 caggcgagtc atacgtgcga tacgacgcat aaacagcagc gatgccagca cgatccccac
     3421 gctgatggca ataaccatat caaacagcac ggtcagtgac atacacagca gcatgacgat
     3481 gatgtcatct ttcggcgcat gacgcagcaa gtcgaccact ttgtgcgctt cactcatgtt
     3541 ccacgccacc atcaacagca gggctgccat ggcggaaagc ggcagccagg agagcagtgg
     3601 tgccagtacc agcagggcga gaataaccag aatagagtgg atcaccgccg agatagggga
     3661 agttgccccg gcacggacgt tagcggcaga acgcgcgatg gcagcagtag cggtaatacc
     3721 accaaagaat ggagcgatga tattccccag cccctgaccg accagttcac tattcgcctt
     3781 gtgcttagtc ccggtcatac catccagtac cacagcgcag agcagagatt cgatcgcccc
     3841 gagcattgcc attgagaatg ccgcaggcag cagggtgcga atagaatccc aggttagcgt
     3901 gaattctgaa ttaggcagat cccacggcag caccagttgc ggcagcagtt gcggaatacc
     3961 gttaccctga gaaccatcgg ccaggacgta gtggaattgc gaaccgatgg tagcaacatg
     4021 tccgccgagc aggttaacaa tccccatcac cgcgcaacca gccagcaagg ccggaaggtg
     4081 accgggtaaa cgaatgccca gacgcggcca gaaaaccaga atgccaagcg tcacaatgcc
     4141 aatggcagca tcacccacat taatggtcgg cagcgccata aataatgcgc cgactttttg
     4201 tagataatgt tccgggacat gggccatttg cagaccgaga aaatctttaa tctgcatggt
     4261 accgatggtg atcccgatac ccgaggtgaa acctaaggtg acggaaaccg gaatatactc
     4321 aatcagacga ccaaagcgtg ccagccccat cagaatcaaa aagattcccg acaacaaggt
     4381 cgcaaccagc agtcctgcca gtccaaactg ttgcgaaacg ggatagagaa ttaccacaaa
     4441 tgccgcagtc ggaccggaaa cgctaaagcg tgacccaccc gttagagcaa tgacaatccc
     4501 cgcaacagct gcggtatata aaccgtactg gggggccaca ccactaccaa tagccaacgc
     4561 catcgccagc gggatagcaa taatcccgac ggttatcccg gcaatcaggt cacgggtaaa
     4621 ccgtgcggca gtatattttt ctttccagca aacgtcgatc agagcgcgga aaggcatcac
     4681 atgtgaggaa aatattttgt tcacaataat gtttcatccg tgagcgcatc atctgtcaac
     4741 taaatggcag gtgaaggagg cataggtcat acaaatggat attacagaca aaaaaacccg
     4801 ccgcagcggg tctttgagcc gggttcgatt agtgttcgaa catggcagag atcgattctt
     4861 cgttgctgat acgacgaatc gcttcggcca gcatacctga cagggtcaga gtacgcacgt
     4921 tcggcagtga tttgatttca tcgctcagcg gaatggtatc gcagacaacg acttcatcaa
     4981 ttacagagtt acgcaggttg ttcgccgcgt tgccagagaa gatcgggtga gtcgcgtacg
     5041 caaatacacg tttagcacca cgttctttca gcgcttcagc agctttacac agcgtaccgc
     5101 cagtgtcgat catatcatcg accagtacgc agtcacgacc tgcaacgtca ccgatgatat
     5161 gcatcacctg ggaaacgttc gcacgcggac gacgtttgtc gatgattgcc atatcggtat
     5221 cgttcagcag cttagcgata gcgcgggcac gcacaacgcc gccgatgtcc ggagaaacca
     5281 caattgggtt atccagattc agctgcagca tgtcttccag caggatcggg ctaccaaata
     5341 cgttatcaac cggaacgtcg aagaaaccct gaatctgttc agcgtgcaga tccactgtca
     5401 gcacacggtc aacaccgacg ctggagagga agtctgcaac cactttcgca gtgattggta
     5461 cacgagcgga acggacgcga cggtcctggc gcgcatagcc aaagtagggg ataacagcgg
     5521 tgatacgacc tgcggaagca cgacgcaggg catcaaccat aacgactaat tccatcaggt
     5581 tgtcgttagt aggggcacaa gtggactgga tgatgaaaat atcaccaccg cgtacatttt
     5641 cgttaatttg tacgctgact tcgccgtcgc taaagcgacc tacagcggcg tcgccgagtg
     5701 aagtgtacag gcggttggca atacgttgtg ctagtaccgg ggtggcgtta ccagcaaaaa
     5761 gcttcatatc aggcacgaga agaacctcag gcatgcgtcc attggtggaa agaatctgcc
     5821 gaaaactgtg cgggccaggc atgatccttt ccaggcggtg tattaaagag cgcgatgcaa
     5881 cgtctggaac aaggtgacgt tgtcaccgaa actcagcttg cccggcttaa agcatggctc
     5941 tgtgcaatgg ggaaagatta acgcctttcg ccacaaagcc attgagccat tccggggctt
     6001 gctctagcac ctggcgggct tcagactctg tatcaaattc agcaaagaca caggcccctg
     6061 tcccagtcag gcgtgacggg gcgtattcta acagccagga aagcaccgca tcaacctcgc
     6121 gaaaacgttt tcttgcgata acctcgcaat cattgctgaa ttcacatttt agcaacgttt
     6181 ctattgacct ttttggcgta ttgcgcggga gttcaggatc tttaaaaatc accggagtcg
     6241 gaatacttac accagggtgc gccaccagat accacttctc tggcggatcc accggcgtta
     6301 gtatttcacc aacgccttcg gcaaacgcgg catgcccccg aacaaagaca ggaacatctg
     6361 cgcccagcgt cagccccatt tccgccagct catccatgct tagcccgcat tgccagagat
     6421 gatttaatgc caccaggacc gtcgcggcat tggatgaacc accgccgaga ccgccgccca
     6481 tcggcaaacg cttgtcaatg ctgatattcg caccgcttcc cgtcggaaga cgcccgctgt
     6541 ctgccgcagt tttcatcaac aaccgcgctg cgcgaacgat caggttatct tcatgttcca
     6601 cgccttcaac gggcgttaac agacgaatat ccccatcgtc acgaagctca atgctgatgg
     6661 tgtcgccgta atcaagaaac tgaaacagcg tttgcagcgt gtggtaacca tccgcacgct
     6721 gaccggtaat gtataaaaac agattaagtt ttgccggaga gggccactgt gtccgcatta
     6781 tttcactatc cagttatcca tttttaactt gatgcgttga ccaccgtcgg tgagttccat
     6841 attggctggc atcgcaggtt gcgttttggt gtcataacca ccataaacaa ccttccagtt
     6901 tttgccattc tggctgtagg taatttcgct caggcggtac tggtcgtcca gtttgtagtc
     6961 ggttgcatca cccggtaaac ctaaaatcca ctggcgcaag ctgttgagcg gaattggcat
     7021 tccggtcaat ttgccaatca tctcttcggc gtcatcggcg gtataacgct gacctttatt
     7081 gtcgactaac tgcacgttac ccggttgagc attcagctcc agttccgtgc tgcccaatgg
     7141 gttagtgagc agcagacggt agcgatcctg gccggtttgc tgccagaaaa agcgggcgta
     7201 cactttttgt tggtcagaaa tataagcgaa cgcgccgcga gtctgatact gattaagatt
     7261 gcgcacgtct tgctgatgct gacgccattg tggcgaatcc gggcttttgc caggaccttt
     7321 gggcgtggta acggaacagg cagtgagcac aagagcagcc agcggtagca ggcggataag
     7381 acgaaaatcg ggcaggggca tagtgatgac aagtccttga gatacgttgc agttataacc
     7441 cttaatgcta gcgttaccgt ccgctatcgt ctatgttcaa gttgtcttaa ttgccagaat
     7501 ctaacggctt tcggcaatta ctccaaaagg gggcgctctc ttttattgat cttacgcatc
     7561 ctgtatgatg caagcagact aaccctatca acgttggtat tatttcccgc agacatgacc
     7621 cttttagcac tcggtatcaa ccataaaacg gcacctgtat cgctgcgaga acgtgtatcg
     7681 ttttcgccgg ataagctcga tcaggcgctt gacagcctgc ttgcgcagcc gatggtgcag
     7741 ggcggcgtgg tgctgtcgac gtgcaaccgc acggaacttt atcttagcgt tgaagagcag
     7801 gataacctgc aagaggcgtt aatccgctgg ctttgcgatt atcacaatct taatgaagaa
     7861 gatctgcgta aaagcctcta ctggcatcag gataacgacg cggttagcca tttaatgcgt
     7921 gttgccagcg gcctggattc actggttctt ggggagccgc agatcctcgg tcaggttaaa
     7981 aaagcgtttg tcgattcgca aaaaggtcat atgaaggcca gcgaactgga acgcatgttc
     8041 cagaaatctt tctctgtagc gaaacgcgtt cgcactgaaa cagatatcgg tgccagtgct
     8101 gtgtctgtcg cttttgcggc ttgtacgctg gcgcggcaga tctttgaatc gctctctacg
     8161 gtcacagtgt tgctggtagg cgcgggcgaa accatcgagc tggtagcgcg tcatctgcgc
     8221 gaacataaag tacagaagat gattatcgcc aaccgcactc gcgaacgtgc ccaaattctg
     8281 gcagatgaag ttggcgcgga agtgattgcc ctgagtgaga tcgacgaacg tctgcgcgaa
     8341 gccgatatca tcatcagttc caccgccagc ccgttaccga ttatcgggaa aggcatggtg
     8401 gagcgcgcat taaaaagtcg tcgtaaccaa ccaatgctgt tggtggatat tgccgttccg
     8461 cgcgatgttg agccggaagt tggcaaactg gcgaatgctt atctttatag tgtggacgat
     8521 ctgcaaagca tcatttcgca caacctggcg cagcgtaaag ccgcagcggt tgaggcggaa
     8581 actattgtcg ctcaggaaac cagcgaattt atggcgtggc tgcgagcaca aagcgccagc
     8641 gaaaccattc gcgagtatcg cagccaggca gagcacgttc gcgatgagtt aaccgccaaa
     8701 gcgttagcgg cccttgagca gggcggcgac gcgcaagcca ttatgcagga tctggcatgg
     8761 aaactgacta accgcttgat ccatgcgcca acgaaatcac ttcaacaggc cgcccgtgac
     8821 ggggataacg aacgcctgaa tattctgcgc gacagcctcg ggctggagta gcagtacatc
     8881 attttctttt tttacagggt gcatttacgc ctatgaagcc ttctatcgtt gccaaactgg
     8941 aagccctgca tgaacgccat gaagaagttc aggcgttgct gggtgacgcg caaactatcg
     9001 ccgaccagga acgttttcgc gcattatcac gcgaatatgc gcagttaagt gatgtttcgc
     9061 gctgttttac cgactggcaa caggttcagg aagatatcga aaccgcacag atgatgctcg
     9121 acgatcctga aatgcgtgag atggcgcagg atgaactgcg cgaagctaaa gaaaaaagcg
     9181 agcaactgga acagcaatta caggttctat tactaccaaa agatcctgat gacgaacgta
     9241 acgccttcct cgaagtccgt gccggaaccg gcggcgacga agcagcgctg tttgcaggcg
     9301 atctgttccg tatgtacagc cgttacgccg aagcccgccg ctggcgggta gaaatcatga
     9361 gcgccagcga gggtgaacat ggtggttata aagagatcat cgccaaaatt agcggtgatg
     9421 gtgtgtatgg tcgtctgaaa tttgaatccg gcggtcatcg cgtgcaacgt gttcctgcta
     9481 cggaatcgca gggtcgtatt catacttctg cttgtaccgt tgcggtaatg ccagaactgc
     9541 ctgacgcaga actgccggac atcaacccag cagatttacg cattgatact ttccgctcgt
     9601 caggggcggg tggtcagcac gttaacacca ccgattcggc aattcgtatt actcacttgc
     9661 cgaccgggat tgttgttgaa tgtcaggacg aacgttcaca acataaaaac aaagcgaagg
     9721 cgctttcagt gctcggtgct cgcatccacg ctgctgaaat ggcaaaacgc caacaggccg
     9781 aagcgtctac ccgtcgtaac ctgctgggga gtggcgatcg cagcgaccgt aaccgtactt
     9841 acaatttccc gcaggggcgc gttaccgatc accgcatcaa cctgacgctc taccgcctgg
     9901 atgaagtgat ggaaggtaag ctggatatgc tgattgaacc gattatccag gaacatcagg
     9961 ccgaccaact ggcggcgttg tccgagcagg aataatggaa tatcaacact ggttacgtga
    10021 agcaataagc caacttcagg cgagcgaaag cccgcggcgt gatgctgaaa tcctgctgga
    10081 gcatgttacc ggcagagggc gtacttttat tctcgccttt ggtgaaacgc agctgactga
    10141 cgaacaatgt cagcaacttg atgcgctact gacacgtcgt cgcgatggtg aacccattgc
    10201 tcatttaacc ggggtgcgag aattctggtc gttgccgtta tttgtttcgc cagcgacctt
    10261 aattccgcgc ccggatacgg agtgtctggt ggagcaggca ctggcgcggt tgcctgaaca
    10321 accttgccgt attctcgatc tcgggacggg taccggggcg attgcgcttg cgctggctag
    10381 cgagcgcccg gactgcgaaa ttatcgctgt agatcgtatg cctgatgctg tctccctggc
    10441 acaacgtaat gcccagcatc tggcgatcaa aaatatccac attctgcaaa gcgactggtt
    10501 tagcgcgcta gccgggcagc agtttgcgat gattgtcagc aatccgccgt atattgacga
    10561 gcaggaccca catcttcaac aaggcgatgt ccgctttgag ccgctcactg cgctggttgc
    10621 ggcagacagt ggaatggcag acatcgtgca tatcatcgaa cagtcgcgta acgcgctggt
    10681 atccggcggc tttctgcttc tggaacatgg ctggcagcag ggcgaagcgg tgcgacaagc
    10741 atttatcctc gcggggtatc atgacgtcga aacctgccgt gactatggtg ataacgagcg
    10801 cgtaacgctc ggccgctatt atcaatgaca agtttttcta cactgcttag tgttcatctt
    10861 attagtatcg cgctttctgt tgggctatta accttacgtt tctggctacg ttatcagaag
    10921 catccacagg catttgctcg ctggacgcgc attgtgccgc cggttgtcga tacggtgtta
    10981 ttgttaagcg gcattgcgtt gatggctaaa gcgcacatcc tgccattttc cgggcaggca
    11041 cagtggctga ctgaaaagct gtttggagtt atcatttata tcgttttggg ttttattgca
    11101 ctcgattatc gtcgtatgca cagtcagcag gcgcgcatta ttgccttccc gctggcgttg
    11161 gtggtgctgt acatcatcat taaactcgcc accacaaaag taccgttact ggggtaagtc
    11221 atgagatcgt tagctgattt cgaatttaat aaagcgccat tgtgcgaagg catgatcctg
    11281 gcttgcgaag caatccgccg cgattttccc tcgcaagatg tttacgacga actggagcgt
    11341 ctcgttagtc tggcgaagga agaaatcagc cagcttctgc ctttagaaga gcagctggaa
    11401 aaactgatcg cgctgtttta cggcgactgg ggatttaaag cctcacgcgg cgtttatcgt
    11461 ctttccgatg cattatggct ggaccaggtg ttaaagaatc gacagggcag tgcggtatca
    11521 ttaggtgcgg ttttattatg ggtcgcgaat cgtctcgatt tgccgctgct gccggtgatt
    11581 ttccctacgc agctgatatt gcgcattgaa tgtccggatg gcgaaatttg gctgattaat
    11641 ccttttaacg gtgaatcgtt aagcgaacat atgctggacg gatggttaaa gggaaatatc
    11701 agcccgtcgg cggaactgtt ttatgaagac cttgatgaag ctgataacat tgaggtaatc
    11761 cgcaaattgc tggatacact caaagcctcg ttgatggaag aaaatcagat ggagctggcg
    11821 ttacgcacca gcgaagcttt attacaattc aaccctgaag atccctatga aattcgcgat
    11881 cgtgggttga tttatgcgca actggattgc gaacacgttg cgttgaacga tttaagttat
    11941 ttcgttgaac ggtgtccgga agacccgatc agcgaaatga tccgtgcgca aataaataac
    12001 atcgcgcata aacatattgt gctgcattaa ttaatcgaca ttttactcaa gattaaggcg
    12061 atcctatgaa acaaaaagtg gttagcattg gcgacatcaa cgtagcaaat gacctgccgt
    12121 tcgtactgtt tggcggtatg aacgtgctgg aatctcgcga tctggcgatg cgcatttgcg
    12181 agcactacgt aactgtgacc cagaaactgg gtatccctta cgtgttcaaa gcctcttttg
    12241 acaaagccaa ccgctcctcc atccactctt atcgtggacc gggcctggaa gaagggatga
    12301 aaatcttcca ggagttgaag cagacttttg gcgtgaaaat tatcaccgac gttcacgaac
    12361 caagtcaggc acagcccgtt gctgatgtcg tggatgtgat tcagttgccg gcgtttcttg
    12421 ctcgccagac tgacctggtt gaagccatgg cgaaaaccgg tgcggtaatt aacgtcaaga
    12481 aaccacagtt tgtcagcccg ggacagatgg gtaatatcgt tgataaattc aaagaaggcg
    12541 gcaacgaaaa agtgattctt tgcgatcgtg gtgctaactt cggctatgac aacctggttg
    12601 tcgatatgct gggcttcagc attatgaaga aagtgtctgg taactcgccg gtgattttcg
    12661 acgtgaccca cgcactgcaa tgccgcgatc cgtttggcgc agcttccggt ggtcgtcgtg
    12721 ctcaggtggc tgagctggca cgagccggta tggcggtagg tctggcgggg ctgtttattg
    12781 aagcgcatcc ggatccggaa catgcgaaat gtgatggtcc atccgcgctg ccgctggcta
    12841 aactggaacc gttcctcaag cagatgaaag cgattgatga tctggtgaaa ggtttcgaag
    12901 aactggatac cagcaagtaa tcttttttgc ttgaaaaata aagtcttagc gttctgcgtt
    12961 aagacttttt tcatgggtgc cagatacaaa aaaggccgca ggctgttacc cctgcggccg
    13021 gtttcgggcg catattgcca tcacggcagc ctgacgcccg ttttcacctt acttccggtt
    13081 acgccaccag ctgacaatcg ctgcggcaat aatccccgcc aggatcggtg ctgccaggtc
    13141 gtgccagaaa gtcatggcaa actgcgcgag cgtcatatag ccgccttgtt gtaatgacaa
    13201 cattttgcgg ctattcttga agtgtctggt ttcaagatta gcccccgttt tgttgtcagg
    13261 ttttacctct caacgtgcgg gggttttctc tttccagcaa ccaatgccac cagggataaa
    13321 gcccccgcaa cattgcgcct caccggataa ttccggcttg gtgtggatac tacgtctcaa
    13381 ttcatcttca cttcatccct gaaatgtttg caataaagag tacattccgg cttttcaaca
    13441 gctgttgcag tggtttcgtg caggttgtgg tacaggctcg caattctgat taacgagctg
    13501 tccaggtagt atgaagaaat acaataagca ggaatttatc ttatctctcc cgatttccgt
    13561 catcggagga gatataactg tcaggcaaat atcgtcatca aataggcggc aaacagtgcc
    13621 agatgcgctg cgccattgag cacgttagta cgtccggtgg agaaggagat atggcacagc
    13681 actaaagagg ccaccatcac caccatttct ggcgcaccaa gtgcaaactg caattcgtta
    13741 cccgtcataa aggcaattag cgtgacgaca ggtacggtaa gcgaaattgt tgctaacact
    13801 gaaccaaaga acagattcat cgcgcgctga acctggttgt tcaacactgc ttttaacgca
    13861 cctaaacctt ccggcgacag aatcaacagt gccaccagga agccagtaaa ggcaacaggg
    13921 gcattcatgc tgtcgagcaa tgtctccagc gagctggcgt tcattttggt gactgcaata
    13981 acggcaatca gatggataat caaccagata gcatgccaca ggctgctatg ggcagacgtt
    14041 ttaccgtggt gcgggtcgtc gtcatcactg tcatcttcgt gctcgtagac aaacaaactt
    14101 tgatgcgttt tggcctggat cagcaaaaat acgccataca ttgccgcaga aattaatgct
    14161 accagtaacg cctgaccggt tgaaaaattc gccgcaggca gagccattgg aaataccagt
    14221 acgattatcg ccagggggaa cagcgcaatt aaatactgct tgataccaaa cagattcata
    14281 tattgggtgg caaacttacg accgcccaac aataatgaaa agccaaccag cccaccggta
    14341 acaatcataa tgattgaata gagcgtatca cgcattagcg ttggcgcggc gtcgccggtt
    14401 gccattaaag ctgaaatcaa actgacttca agaataacca ctgaaaggct aagaataagc
    14461 gaaccgtaag gttctcccag gcgatgggct aatacgtccg catgacggac aacactaaag
    14521 gcgctactta aaataccaat aagcgcaaga agattgatgg caatgaccac tggtagtgtc
    14581 tggctacttc cccacaggaa cagcactacc agcaccagaa ccgggaaaat aagcgaagtc
    14641 tccttgtggc gggtttttac cgcctcttga gcatttgaca ttatggttat ccctttgcag
    14701 atgaatttat cgaaaatgta aaaaataggt aggaaaaata acagaaagtg tctggatatc
    14761 ggtaacattt tacgaatttt taccccgctg tcgatttttt actcattggg gcataaaaat
    14821 aagtattacg tttagacaat gtttgtttta gcgtttctta agaacagtct gacatgaaaa
    14881 ttcttatgtt ttggcaagag tagatattgt tgaccacact taatgttcaa ctttgtaaaa
    14941 ggagtcaacg atgccgtata aaacgaaaag cgatctgccg gaaagcgtaa agcacgttct
    15001 accgtctcat gcccaggata tctataaaga agcgttcaac agcgcatggg atcaatataa
    15061 agataaagaa gatcggcgtg atgacgccag tcgcgaagaa accgcgcata aagtggcctg
    15121 ggctgctgtg aagcatgaat atgccaaagg ggatgatgat aaatggcata aaaaatcgta
    15181 aaaccggtag cttagttaaa gctattcgtg cggtgttgcc ttgcaagtgg tccgtggatt
    15241 gcatattgtt ccgttagtgg tttcaaaatg agcggtaaaa atgtccggaa gacaccaaaa
    15301 agttgtcgca gggaagtatg cagtggcgga ggtgtaaggt gataacgcgt gatttcttga
    15361 tgaatgccga ttgtaaaacg gcatttggtg ccattgaaga atcactctta tggtcagcag
    15421 aacaacgggc ggcttcgctg gcggcgacgc tggcttgtcg acctgatgag ggaccggtgt
    15481 ggatcttcgg ttatggatcg ttgatgtgga atccggcact ggagtttacc gaatcatgca
    15541 ccggtacact ggttggatgg catcgcgcgt tttgcctgcg cctgaccgcc gggcgcggga
    15601 ctgcgcacca gccgggacgg atgcttgcac tgaaagaggg cggacgcacc acaggcgtcg
    15661 cctatcgact accagaagag acgctggagc aggaactaac cctgttgtgg aagcgcgaga
    15721 tgattaccgg ctgttatctg ccaacctggt gtcagcttga tcttgatgat ggacgcacag
    15781 taaacgccat tgtgtttatt atggacccgc gacatccaga atatgaatct gacactcgcg
    15841 ctcaggtcat cgcgccgttg attgcggcgg cgagcggtcc gctgggaacc aatgcacaat
    15901 acctgttttc actggaacag gagcttatca aactgggaat gcaggacgat gggctgaatg
    15961 atttgctggt atcggtaaaa aaactactgg cggagaatta tccggatggt gtgttaaggc
    16021 cgggattcgc ctgagtaaac ttccctcata gtggggcgtc agacgccccc ttaaacatta
    16081 aaatgtgagc actttatcgg ctgacagcgt ccattgcgcc agttccacaa gagtaccgat
    16141 ttccacccca tcaatcagag gaagtgtgct aatcccgcgc ccgtcggtac aggttttgca
    16201 caatttcacc ggtacattct gagcggtaag gatctccagc atttgctgaa tgttgtagcc
    16261 ttcccctggt ttttgcccgc gcaacccggc tgtgaccgca tcagacatca ggaacagacg
    16321 cagatccaga ttgctctcct gctctcgtaa cgcaatggcc agccgcaagc tgttaaacaa
    16381 ggattcgctc ccgtaaggtg cgccattggc aacgatcact attttttgca ttatttactc
    16441 ctgtattcag ggaattagac actcatcttc tatcttactg cttctgcagc gtctgaccaa
    16501 tcggtcacat ttttaaggat tttcctgaaa gcgcgagaaa atacgacaaa agttgccagt
    16561 aatcgttatt ctttaaggct atggtttttc attattaccg gaagttaccg acgttttgag
    16621 ccgtttcgtt cctcgcatta tcccgtttta tttactcttg cttgtagcag gcggtacagc
    16681 taacgcacaa tctaccttcg agcaaaaagc ggcaaatccc tttgataata acaatgatgg
    16741 tctgccggat ttaggcatgg ctcccgaaaa tcatgatggg gaaaaacact ttgctgaaat
    16801 tgtgaaagat ttcggcgaaa ccagtatgaa tgataacggg ctggatactg gcgagcaggc
    16861 aaaagctttc gcattgggaa aagtccgcga cgcgcttagt caacaggtta atcagcacgt
    16921 agagtcctgg ctatcaccgt ggggaaatgc cagtgttgac gtcaaagtgg ataacgaagg
    16981 acatttcacc ggcagtcgtg gaagctggtt tgtgccgtta caagataatg atcgttatct
    17041 cacctggagc cagcttggtc ttactcagca ggataatggg ttggtgagca atgtgggcgt
    17101 tgggcaacgc tgggcgcgcg gcaactggct ggtgggttat aacacttttt atgacaactt
    17161 gcaggacgaa aatcttcagc gagcgggctt tggtgccgaa gcgtggggcg aatatttgcg
    17221 attatcggca aacttttatc agccgtttgc tgcatggcat gaacagacag ccacgcagga
    17281 acaacggatg gcgcgcgggt acgacctgac agctcggatg cgcatgccgt tctatcaaca
    17341 cctcaatacc agtgtcagcc tagaacagta ttttggtgat cgtgttgatt tgtttaactc
    17401 tggtacgggt tatcacaatc ccgtcgcgtt gagtctggga ttaaattaca cccctgtgcc
    17461 attagtcact gtgacggccc agcataaaca gggggaaagt ggcgaaaatc aaaataacct
    17521 cgggctgaat cttaattacc gctttggtgt accgctcaaa aaacaacttt ctgcgggcga
    17581 ggttgccgaa agtcagtcgt tacgtggtag tcgctatgac aatccgcagc gaaataatct
    17641 accgactctt gagtaccgac agcgaaaaac gttaacggtg tttctggcga caccgccgtg
    17701 ggatctaaaa cctggcgaaa cagtgccgct gaaattacaa atccgcagtc gttacggtat
    17761 tcggcaactg atttggcagg gcgatacgca gatattaagt ttgacgccgg gcgcacaagc
    17821 caacagcgcg gagggctgga cgctgatcat gcctgactgg cagaacgggg aaggggcaag
    17881 caatcactgg cgattgtctg tggtggtgga agataaccag gggcagcgtg tctcctccaa
    17941 tgagatcacg ctaacgcttg tcgaaccgtt cgatgcattg tcaaacgacg aactgcgctg
    18001 ggaaccgtaa tcagaaaatg cgctcctgat gcacccatac cgctgcttcc acgcgagact
    18061 tgagcttcat tttcttcagc atgtgcttga cgtgcacttt tactgtgctt tcggtgatat
    18121 ccaggcggcg ggcaatcatc ttgttcggca aaccctgggc aatcagcttg agaatatcgc
    18181 gctcgcgtgg ggttaactgg ttaacatcgc gctcagtagt ggcacggtta gcgcgcaagc
    18241 tggcggccag aacaggcgtt aatgcttcgc ttaataccat ttcgccagca gctgcctgat
    18301 gcaatgcttt cagcagatct tccggttcca tatcttttaa cagatagcca tccgcgccgc
    18361 gtttcagtgc ggtgaccaca tcttcttcat ggttagagac gctgaatacc acaatgcgcc
    18421 ctgagaggga cttttcgcgc agtttatcca gcgtttccag accgttcatg ccgggcatat
    18481 tgagatctaa caggatcaga tcgggatcaa gagattccgc cagttcaata ccctgttcgc
    18541 cattactcgc ttcgccaacc acggtgatat ctggtgccat actgataagc tgttttacgc
    18601 cagttcgcag catcgggtga tcgtcaatca gcaggatagt agccggttcc tgattactca
    18661 tgggtatctc cttggacgtc tgtgaaagtt ttttcgggaa taaaggtgac caccacttcg
    18721 gtgccacctg attcacgacg gcggacgcgg caatcgcctc gtaaactttg cgcacggtcg
    18781 cgcattatta tcatgccgta gtgattgctg cggatggcat tttcaggcac gccacagccg
    18841 ttatcctgga cggtcagttt gacctgatta tcgttttgcg ccaccgtcac cacgacctca
    18901 ctcgcttgcg aatgtttgag ggcgttactt aatgcctcac gggcaatttg caacaagtgg
    18961 attgcctgat gtgaaggcac cagacgaggc ggcaattgat aatccagctt caccgggaag
    19021 ccaaatttgg cgctgtactc ttcgcaactc gcttccagcg ccggacgtaa tccaggctcg
    19081 gtgagctgca agcggaatgt ggtgagcaat tcacgcaact gcgcccagga tgcattcagt
    19141 tcgttacgga tctgacttaa cagttcgcgg ctgctttctg gcagcgcatc gccctgcatc
    19201 tgtaaacaac tcacctgcat cttcatgcaa gagagagatt gggcaataga atcatgcagt
    19261 tcgcgcgcaa tggtggcacg ctcttccatc acgatcaact gttgctgacg ttcctgatgg
    19321 cgatccagcg ccagcgtggc ggtaagttgt tcaaccaggg tatccaccag ttgttgttgg
    19381 tcatggctaa gatgacgccc ctgcggcagg gtcgccagca aaataccgta ctgcgtatga
    19441 gagtcagcca gccgccactt cagggtcgtg ccgcgatcac caacgggtaa tacgccgcgc
    19501 gggcagagct ggcagccttt atcatcacaa gtcatatctg gctggcaggt aaactcctga
    19561 tgattctctt catcatcagt gtcatacacc cgcagttcga tatcacgtag cagggttaaa
    19621 ttctgtaagc cgttgagtac cggtgacagg cgttcacaca gcggggcgcg ggaatgcaaa
    19681 cggcggttag cctgccataa aaaagagagg atctgatttt tatgctccag cccggcggtt
    19741 ttctcctgaa cccgctgctc aagtacggca taactttcgg ccagttctgc agacatattg
    19801 ttcaacgcag ttccaagcat cgccatttcg ttgcgcccgc tgatgtttgc gcgttgggta
    19861 aaatcgcgat ggctgacggc actcgccatt gccagcagtt gccgccacgg ttgtagcagt
    19921 cgcgcccgca accagataat agtgaacacc agtaaaagtg ccataaatac cgccattacc
    19981 cgatggacca gtaccactgt ctcaatacgc atttccgtgg tgcggtcaaa accagatacc
    20041 agctgatcaa gcccggcaac aaactggctg acatccgctg acaccgtttc tcggttttgt
    20101 gcacgcatca gcgcagggat cagttcatta cgccagtaat cttgtaagcc ctgtaattgc
    20161 gccagttgtc cgtctcgttc tgctgctcga gtcaactcgg cgctaaatgc cgtttgttcc
    20221 atctctttaa ttaagggctt gtctttctcg cttaatggca ctgccgccaa cagacggtaa
    20281 ctttgcatgc gcagcgatcc cgctttgttg atcgcatggg cgctgccctg aacgccttgc
    20341 accagccagc cagaaaccgc catccctgcc agtccaatag cagtagaaag caacacaata
    20401 agcgcaacct gattaaccag ggtgagcgga gagagacaac gtttaagcat gtaaacccct
    20461 tccttcaggc tttaaatgag caataacctt aatgaatgtg acgatatatt ctggaatggc
    20521 agtattctcg gctattggct gaagtatacc catagccgga aagagttact ccttatttgc
    20581 cgtgtggtta gtcgctttac atcggtaagg gtagggattc tacagcaccg tgaaaaatct
    20641 cataattttt atgaaatcac tgtactcact atgggtaatg ataaatatca atgatagata
    20701 aagttatctt atcgtttgat ttacatcaaa ttgcctttag ctccagacac taaggtggca
    20761 gacatcgaaa cgagtatcag aggtgtctat gagtcactca tctgcctccg aaagggctac
    20821 tggagctgtc ataacagatt ggcgaccgga agatcctgcg ttctggcaac aacgcggtca
    20881 acgtattgcc agccgcaacc tgtggatttc cgttccctgt ctgctgctgg cgttttgcgt
    20941 atggatgttg ttcagcgctg ttgcggtgaa cttaccgaaa gtcggtttta attttacgac
    21001 cgatcagcta tttatgttga ctgcgctgcc ttcggtttct ggcgcgttat tacgtgttcc
    21061 atactccttt atggttccta tcttcggtgg tcgtcgctgg acggcgttca gcaccggtat
    21121 tctgattatt ccttgcgtct ggctgggttt tgccgtgcag gatacctcca cgccttatag
    21181 cgtcttcatc atcatctctc tgctatgcgg ctttgctggc gcgaacttcg catccagtat
    21241 ggcaaacatc agcttcttct ttccgaaaca gaagcagggt ggcgcgctgg gtctgaatgg
    21301 tggtctggga aacatgggcg tcagcgtcat gcagttggtt gctccgctgg tggtatcact
    21361 gtcgattttc gcagtatttg gtagccaggg cgtcaaacag ccggatggga ctgagctgta
    21421 tctggcgaat gcgtcctgga tatgggtgcc gttccttgcc atcttcacca ttgcggcgtg
    21481 gtttggcatg aacgatcttg ctacctcgaa agcctccatc aaggagcagt tgccggtact
    21541 caaacggggt catctgtgga ttatgagcct gctgtatctg gcaaccttcg gctccttcat
    21601 cggcttctcc gcgggctttg cgatgctgtc aaaaacgcag ttcccggatg ttcagattct
    21661 gcaatacgct ttcttcgggc cgtttattgg tgcgctggcg cgttctgcag gtggtgcatt
    21721 atctgaccgt ctgggcggaa ctcgtgtcac gctggtgaac tttattctga tggcgatttt
    21781 cagcggcctg ctgttcctga ccttaccgac tgacgggcag ggcggaagct tcatggcgtt
    21841 cttcgcggtc ttcctggcgc tgttcctgac agctgggctg ggtagtggtt ccactttcca
    21901 gatgatttca gtgatcttcc gtaaactgac aatggatcgc gtgaaagcag aagggggttc
    21961 tgacgaacgt gcgatgcgtg aagcggcaac cggcacggca gcggcgctgg gtttcatctc
    22021 tgcgattggc gcgattggtg gcttctttat cccgaaagcg tttggtagct cgctggcatt
    22081 aacgggttcg ccagtaggtg caatgaaagt atttttgatt ttctatatcg cctgcgtagt
    22141 gattacttgg gcggtatatg gtcggcattc taaaaaataa atcttaatat t
//
LOCUS       DAZSIO010000055        22061 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZSIO010000055 DAZSIO010000000
VERSION     DAZSIO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.055"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            51..548
                     /gene="ftnA"
                     /locus_tag="ACHX5T_001919"
     CDS             51..548
                     /gene="ftnA"
                     /locus_tag="ACHX5T_001919"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR8467146.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(585..824)
                     /locus_tag="ACHX5T_001920"
     CDS             complement(585..824)
                     /locus_tag="ACHX5T_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR8467147.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            1014..2225
                     /gene="tyrP"
                     /locus_tag="ACHX5T_001921"
     CDS             1014..2225
                     /gene="tyrP"
                     /locus_tag="ACHX5T_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR8467148.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2287..2952)
                     /gene="yecA"
                     /locus_tag="ACHX5T_001922"
     CDS             complement(2287..2952)
                     /gene="yecA"
                     /locus_tag="ACHX5T_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR8467149.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3148..3234)
                     /locus_tag="ACHX5T_001923"
     tRNA            complement(3148..3234)
                     /locus_tag="ACHX5T_001923"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3198..3200),aa:Leu,seq:taa)
     gene            complement(3247..3320)
                     /locus_tag="ACHX5T_001924"
     tRNA            complement(3247..3320)
                     /locus_tag="ACHX5T_001924"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3286..3288),aa:Cys,seq:gca)
     gene            complement(3375..3450)
                     /locus_tag="ACHX5T_001925"
     tRNA            complement(3375..3450)
                     /locus_tag="ACHX5T_001925"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3415..3417),aa:Gly,seq:gcc)
     gene            complement(3602..4150)
                     /gene="pgsA"
                     /locus_tag="ACHX5T_001926"
     CDS             complement(3602..4150)
                     /gene="pgsA"
                     /locus_tag="ACHX5T_001926"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR8467150.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4207..6039)
                     /gene="uvrC"
                     /locus_tag="ACHX5T_001927"
     CDS             complement(4207..6039)
                     /gene="uvrC"
                     /locus_tag="ACHX5T_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR8467151.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6036..6692)
                     /gene="uvrY"
                     /locus_tag="ACHX5T_001928"
     CDS             complement(6036..6692)
                     /gene="uvrY"
                     /locus_tag="ACHX5T_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR8467152.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            6834..6905
                     /locus_tag="ACHX5T_001929"
                     /pseudo
     CDS             6834..6905
                     /locus_tag="ACHX5T_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            6988..7164
                     /gene="yecU"
                     /locus_tag="ACHX5T_001930"
     CDS             6988..7164
                     /gene="yecU"
                     /locus_tag="ACHX5T_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR8467153.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            7151..7375
                     /gene="yecF"
                     /locus_tag="ACHX5T_001931"
     CDS             7151..7375
                     /gene="yecF"
                     /locus_tag="ACHX5T_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR8467154.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7408..8028)
                     /gene="sdiA"
                     /locus_tag="ACHX5T_001932"
                     /pseudo
     CDS             complement(7408..8028)
                     /gene="sdiA"
                     /locus_tag="ACHX5T_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(8258..9010)
                     /gene="tcyN"
                     /locus_tag="ACHX5T_001933"
     CDS             complement(8258..9010)
                     /gene="tcyN"
                     /locus_tag="ACHX5T_001933"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR8467155.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(9007..9675)
                     /gene="tcyL"
                     /locus_tag="ACHX5T_001934"
     CDS             complement(9007..9675)
                     /gene="tcyL"
                     /locus_tag="ACHX5T_001934"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR8467156.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(9690..10676)
                     /gene="dcyD"
                     /locus_tag="ACHX5T_001935"
     CDS             complement(9690..10676)
                     /gene="dcyD"
                     /locus_tag="ACHX5T_001935"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR8467157.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(10781..11581)
                     /gene="tcyJ"
                     /locus_tag="ACHX5T_001936"
     CDS             complement(10781..11581)
                     /gene="tcyJ"
                     /locus_tag="ACHX5T_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR8467158.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(11669..12220)
                     /gene="fliZ"
                     /locus_tag="ACHX5T_001937"
     CDS             complement(11669..12220)
                     /gene="fliZ"
                     /locus_tag="ACHX5T_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFR8467159.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(12266..12985)
                     /gene="fliA"
                     /locus_tag="ACHX5T_001938"
                     /pseudo
     CDS             complement(12266..12985)
                     /gene="fliA"
                     /locus_tag="ACHX5T_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(13150..14802)
                     /gene="fliC"
                     /locus_tag="ACHX5T_001939"
     CDS             complement(13150..14802)
                     /gene="fliC"
                     /locus_tag="ACHX5T_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HFR8467160.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            15068..16474
                     /gene="fliD"
                     /locus_tag="ACHX5T_001940"
     CDS             15068..16474
                     /gene="fliD"
                     /locus_tag="ACHX5T_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR8467161.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            16499..16909
                     /gene="fliS"
                     /locus_tag="ACHX5T_001941"
     CDS             16499..16909
                     /gene="fliS"
                     /locus_tag="ACHX5T_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR8467162.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            16909..17274
                     /gene="fliT"
                     /locus_tag="ACHX5T_001942"
     CDS             16909..17274
                     /gene="fliT"
                     /locus_tag="ACHX5T_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR8467163.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            17352..18839
                     /gene="amyA"
                     /locus_tag="ACHX5T_001943"
     CDS             17352..18839
                     /gene="amyA"
                     /locus_tag="ACHX5T_001943"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR8467164.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(18873..19286)
                     /gene="yedD"
                     /locus_tag="ACHX5T_001944"
     CDS             complement(18873..19286)
                     /gene="yedD"
                     /locus_tag="ACHX5T_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR8467165.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            19473..20678
                     /gene="yedE"
                     /locus_tag="ACHX5T_001945"
     CDS             19473..20678
                     /gene="yedE"
                     /locus_tag="ACHX5T_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR8467166.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            20675..20908
                     /gene="yedF"
                     /locus_tag="ACHX5T_001946"
     CDS             20675..20908
                     /gene="yedF"
                     /locus_tag="ACHX5T_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR8467167.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            21015..21685
                     /locus_tag="ACHX5T_001947"
                     /pseudo
     CDS             21015..21685
                     /locus_tag="ACHX5T_001947"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(21725..>22061)
                     /locus_tag="ACHX5T_001948"
     CDS             complement(21725..>22061)
                     /locus_tag="ACHX5T_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR8467168.1"
                     /translation="KSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRCA
                     CCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQE
                     PTEMIQATA"
BASE COUNT         5412 a         5708 c         5347 g         5594 t
ORIGIN      
        1 aaattatagt gacgccacag caacaaatat aacctttgtg gagcactatc atgctgaaac
       61 cagaaatgat tgaaaaactt aatgagcaga tgaacctgga actgtactct tcactgcttt
      121 atcagcaaat gagcgcctgg tgcagctatc ataccttcga aggtgctgcc gcgttcctgc
      181 gccgtcacgc ccaggaagag atgacgcata tgcagcgtct gtttgattac ctgactgata
      241 ccggcaattt accgcgtatt aataccgttg aatctccgtt tgctgaatat tcctcacttg
      301 atgaattatt ccaggaaacc tataaacacg aacaattaat cacccagaaa attaacgaac
      361 tggctcatgc tgcaatgacc aatcaggact acccaacatt taatttcctg cagtggtatg
      421 tttctgagca gcatgaagaa gagaaactgt tcaaatcgat tattgataaa ttaagcctgg
      481 caggcaaaag cggcgaaggt ctgtatttta tcgacaaaga actctctacc ctcgacacac
      541 aaaactaatg ctctccggcg gcaggtttct gccgccgttt caaatcagtg acggcaaatc
      601 ttactctgat cggtcgaaaa accttcttct gaaggtataa atttgccttt tgctgccaga
      661 aacgctacca gctctcccgc tgtcacccct tctgccgagc acgtgtgaaa acgtgcctgt
      721 tcaccaaaac gcgctttaat cgcagcttcc agactggcat gcgtatattg ctcgcctgat
      781 tcaatcatca tatttaacac ttcatgaccg tgaatagagt ccatcgttcc tcctcaaaaa
      841 aagcctagcg tagcgattac cgcttatgaa gactttgcgc cagcgcagga ctgaatgctt
      901 ttattgtaca tttatattta cacaatatgt aacgtcggtt tgacgaagca gccgttatgc
      961 cttaacctgc gccgcagata tcactcataa agatcgtcag gacagaagaa agcgtgaaaa
     1021 acagaaccct gggaagtgtt tttatcgtgg cgggaaccac aattggcgca ggcatgctgg
     1081 caatgccgct ggctgcggcc ggtgttggtt ttagcgttac gttaatcttg ttgattgggc
     1141 tttgggcgtt gatgtgctac acggcgctat tactgctgga ggtgtaccag catgttccgg
     1201 cagataccgg tctgggcacg ctggcaaaac gctatctggg acgctacggt caatggctga
     1261 cgggcttcag tatgatgttc ttaatgtatg ctctgactgc agcatacatc agcggtgccg
     1321 gtgaattgtt ggcctccagc atcagcgact ggacaggtat ttcaatgtcg gcaaccgctg
     1381 gcgtgctgtt gttcactttt gttgccggtg gcgtggtttg tgttggaaca tcgctggtcg
     1441 atttatttaa ccgttttctg ttcagcgcca aaattatttt tctggtggtg atgctggtat
     1501 tgctgctacc gcatattcac aaagtgaatc ttttaaccct gccgttgcaa caggggctgg
     1561 cactgtctgc aatcccggtg atttttacgt cgtttggttt tcacggtagc gtgccgagta
     1621 ttgtcagcta tatggatggc aacattcgta agctacgctg ggtgtttata atcggtagtg
     1681 ccatccccct ggtggcatat attttctggc aggtggcgac gcttggcagc attgattcaa
     1741 caacctttat gggattgctg gctaatcatg ctggattaaa cgggctgtta caggcgttac
     1801 gcgaaatggt ggcctctccg catgttgagc tggcggtgca tttatttgct gatttagccc
     1861 tcgccacgtc atttctcggc gttgcgttag gcttatttga ttatctggct gatttgtttc
     1921 agcgttcaaa taccgttggt ggacgattgc aaactggtgc aattaccttt ctgccgccgt
     1981 tggcgtttgc actgttttat ccacgaggat ttgtgatggc gctgggttac gccggtgtgg
     2041 cgctggcggt actggcattg attatccctt cactattgac ctggcaaagc agaaagcaca
     2101 atcctcaggc gggttaccgg gtcaaaggtg gtcgtccggc gctggtggtg gtgtttctct
     2161 gtggtattgc tgtgattggc gtgcaatttt tgattgcggc agggttgtta ccagaagtgg
     2221 ggtgatcaga tagcctcaaa ttccttattg ggtgccagaa ttaacgctga cacccaatgt
     2281 gacctcttaa tgcaggcagc actgcttaaa tttcttacca ctgccgcacg gacaaggatc
     2341 gttacgcccc ggtttctctt ctgctttgat cggttgctga acagcttttt cctgcggatg
     2401 cgccatccag tatgcatgta aatcaagcgc cgccagtcga atggcatcta cgctctcttc
     2461 aaacgcttct ggcgacattt tctctacccg ctcgaagttt tcctcagtac cgtgcagcgc
     2521 aatcgcctcc agcgctggtt ttaaagaatc gggcaacgtt gaccagtcag aaagtgccac
     2581 gccccgcata tagccaaagc accactcctc aacaatcgtc agctcgctgc catcaacttc
     2641 tcgcaagccg aataacggct caaactgctc cgggaattcg ttcagacgct ctgcggtatc
     2701 ggccatatgt tgaaaagcca ggttcataaa gcgcgtcatc tctttctctg acgcccaacg
     2761 cggcacatag tcagccccac cccacacggc aaccagccac tgttctggtt caatctcttg
     2821 cggagaactc aacaccgccg tcaataaacc gtccagctcc gccacatcaa ggatggcgtg
     2881 gtcagtgttg tatttggtca gaatatcgtc cagccattcc aactcacttt cgtttaacgg
     2941 tcccgttttc atacgctttt ccttgtggat ctcaactcgc cagcacctat cttacatgcc
     3001 ggtccgtatc agagatactt tttgagtggc tttgccggtg attaaaaatt aaggagggtg
     3061 taacgacaag ttgcaggcac aaaaaaacca cccgaaggtg gtttcacgac actgcttatt
     3121 actttgattt tattcttatc tttcccatgg tacccggagc gggacttgaa cccgcacagc
     3181 gcgaacgccg agggatttta aatcccttgt gtctaccgat tccaccatcc gggctcggga
     3241 agaaagtgga ggcgcgttcc ggagtcgaac cggactagac ggatttgcaa tccgctacat
     3301 aaccgctttg ttaacgcgcc aaattcttca ggcctttcag ccagacatcc gcttgacgcc
     3361 gatgtctttt aaactggagc gggaaacgag actcgaactc gagacctcga ccttggcaag
     3421 gtcgtgctct accaactgag ctattcccgc attcatcaag caatcagtta atcacttgat
     3481 tttattatcg tctggcaatc agtgccgccg ttcgatgcgt tgcattctac ttacctggcg
     3541 cgatgagtca acgatatttt tcaccacttt tgatcgtttg ctgaaaatta cgccgaaacg
     3601 atcactgatc aagcaaatct gcacgcgcag cgctcaaata ttgcaacatt gaccacagag
     3661 tcagtaccgc agccacaaag aaaagtgcaa taccgacgta ctcaacccaa atgttcggac
     3721 gccacagcag ccaggccaac gccaccatct gggcagtggt tttcactttc ccaatccagg
     3781 agacggccac gctactgcgt ttacccaact ccgccatcca ttcgcgtagc gcagaaataa
     3841 taatttcacg ggcgatcatc gttgccgccg gtaatgtcac ccaccagctg tgataatgct
     3901 cggttaccag caccatggcg atagccacga gaactttatc tgccacaggg tcaaggaaag
     3961 caccaaaccg ggtactctgg ttccagcggc gtgccagaaa accatcgaac cagtcagtca
     4021 ccgccgcgac gcagaaaatg agcgcggcgg caaacggcga ccaggtgaca ggcagataaa
     4081 agaccaatac aaagaatggg ataaggatga cacggaacag tgtaagcaac gtagggatat
     4141 taaattgcat aatgacgggt aactatctgt tgtcagtaag attaccccta tgttgctaca
     4201 gagacatcaa tgtttcaacg accagaagat cttttctgcc agaccttgcg aaatacccgg
     4261 cacttttgca atttcctcga cgctggcgtt acgtaaacct tgcaaaccgc ccatatattt
     4321 caacaacatt tgccgacgtt ttggcccgac gccttcaatg gtttccaggg aactggtatt
     4381 tttgaccttc gcccgttttt tacggtgccc gccaatcgcg tgatcatgtg attcatcgcg
     4441 aatatgctgg ataacatgca gcgcgggtga atctggcggc aaactaaatc cctcaccttc
     4501 cggctcaaag aacagcgttt ccagtccagc cttacgatct gctcctttgg caacgccaag
     4561 tagcagcgga tgatttttat cccatgagac atccagttcg gcgaagacat ttttcgcctg
     4621 cgcaagctgg cctttgccgc cgtcgataag gatcacatcc gggatcttac tgtcgtcaat
     4681 ggctttacca taacgccgac gcagcacctg attcatcgcc gcataatcat cgcccggcgt
     4741 gatgcctgta atgttatagc gccgatactc cgcacgcagc gggccgttag catcaaacac
     4801 cacacaggaa gcgacggttt gttcgcccat ggtatggctg atgtcaaagc actccatccg
     4861 cttcacttcc ggcaatttca acacgctggc aagcgcggtc agtcgctggt gaacggtaga
     4921 ttgctgcgaa agtttgctgg ttaaggccgt cgccgcattg gtgcgcgcga gtttcagata
     4981 acgcgcccta tcgccgcgag gtttggtttg aacattaatc ttgcgtcccg ccagttctga
     5041 aagggaatcg gcgagcagcg ttttatcgct aagattaaaa tcgagcagga tctcacccgg
     5101 taaggtgcgc atctggctgc cttgtaaata gaactgacct acgaaggttt ctaccacctc
     5161 gctcagttcc gtaccgccag gcactttcgg gaaatagctg cggctgccga gcactttgcc
     5221 ctgacgaatg aacaatacgt ggacacaagc catgcccgca tcgaacgcca caccaataac
     5281 gtcaaggtcg tcgccggtat tggaaacaaa ctgtttttcg gtgacgcgtc gcaccgcctg
     5341 aatttggtcg cgaatacatg ccgcttcttc aaactccaga ttctggctgg cagtttccat
     5401 acggctaatg agttgcgtaa gcacctgatc atctttgcca gacaaaaaca ggcgcacata
     5461 ctcgacctgc tgagcgtatt cttcttcact caccagtcct tcaacgcacg gtcccagaca
     5521 gcgccctatc tggtattgca gacacggacg cgagcgattg cgataaacac tattttcgca
     5581 ctggcgaatg gggaaaatct tttgcagtag cgccagtgtt tcacgtacgg catagccatt
     5641 cgggaacggg ccgaaatatt cacctttggc atgctttgca ccacgatgca tcgccagacg
     5701 cgggtgggta tcgccactca ggaagataaa aggataagat ttatcatcgc gtagcaaaac
     5761 gttgtaacgc ggctgataga gtttgatgta gttgtgttcc agcaacagcg cttcggtttc
     5821 tgtatgcgta accgttacat caatttgctg gatctgggcg accagcgctt cggttttgcg
     5881 cgaagcgagg ttgctgcgga aatagctgga aagccgtttt ttcaggtctt tcgctttgcc
     5941 gacatagata accgtaccac cagcatcgta catgcgataa acgcctggct ggctggttac
     6001 ggtttttaaa aacgcttttg cgtcaaactg atcactcact gacttgataa tgtctccgca
     6061 ttacacagac catggcgaat tgccaggtga gtcagctcaa catcgccatg aatgtttagt
     6121 ttactgaaca tacgatagcg gtagctgttc accgttttcg gactgagatt gagctgttct
     6181 gagatctcat tgaccttctg gcccttagtg atcatcagca taatctgcaa ttcacgttca
     6241 gacaaactgg caaatgggct ttctgttttt tctggttcga tctggcttaa cgccatttgt
     6301 tgagcgatat cagaagcaat gtaacgctgc cctgaataga cagaacgaat cgcactcacg
     6361 acttcctgcg gagccgcgcc tttgctgagg tagccagcag cacctgcctg catgactttc
     6421 gctggtaaag ggttttctgt atggaccgta agcatgatga ttttaacatc agctgtggaa
     6481 cgcgcgattt tacgcgtcgc ctcaagaccg ccaatgcccg gcatactcat gtccattagc
     6541 accacgtcaa cggcatttgt ccggcaccac ttaacggcgt cttcaccgca cgatgcctct
     6601 ccgacgactt ttataccctt aatatcttcc agaatgcgtc gtatccctgc gcgcaccagt
     6661 tcgtggtcat caacaagtag aacgttgatc aaaggaatat ctccagaaat agggataacg
     6721 ctactgatag ttagtcattc gtatattagc ggtttttatt gcaactttga aacgttaaaa
     6781 atggtgggta ttcgattttt ctctcgtttt tgtcaattcg attgtccaca gttatggtca
     6841 cgcccgtcac caacggctta ccggatcgtt tcacagtaag ccctacgatg aaatgtgaca
     6901 aaaattgact ttattcagca aaaatgaaaa tcagcccgct gaatatgtaa cattaattaa
     6961 cccgaacaat acgggcaaaa aacattcatg ataaaaatat ttattggtca ttatattaac
     7021 gtattttata gcactgccga tatcacgctc aaaaaacaac cactgctatt tttagcaaag
     7081 cttatggtat actccgccgc cttaacattt ttcaccacaa attttcattg caacatgacg
     7141 aggaaaataa atgagtacgc ctgatttttc tactgccgag aataatcaag aactggcaaa
     7201 tgaagtctcc tgcctgaaag cgatgctgac gctgatgctg caggcgatgg gacaagctga
     7261 cgcgggccgc gtgatgttaa agatggaaaa acagcttgcg ctgatcgaag acgaaaccca
     7321 ggctgcagta ttttccaaaa cggttaagca aattaaacaa gcctaccgtc agtaatataa
     7381 aaccggctga gagcgtgcct ttcagccggt tattgacaaa atcatcgcta tctctgctga
     7441 tgtcttccct tccgccgtcc atttcagaat ttctttttcg cgcttgctga aattcatctc
     7501 tggcgtcatc actatttcat catttaaacg catcagagcc atcagacttt cgcgcaccag
     7561 taactgcatt tttaattgca gttcatcact aagaatgggt atttcgcgcg tgctgcaacg
     7621 ggaaaaggac aaaaagccca gcgcccgatt tggcagcatt aaatactgag tgacaccgcg
     7681 gcgtaaacca tgtgcgtgcg cggcttccca taatggctgt gcttcgctga ataagtcatc
     7741 attccacatt aaatggccct gactaaagtt ttcagggttc agcacaggat caattgcgag
     7801 aaaatttttt gcctgataat aactaaccca cgactcaggg taattggtgt agaaagccac
     7861 tttaggtcgc gtgaatggta ccgggtggcg aacacataac gaatagtaat cgtactccag
     7921 ctgctgagcc tgaagctcta tttcatggta ggcctcttct gcggcctcca tcctctgaaa
     7981 acgcaacagc atcgtgcgac gccagctgaa aaaatccgta tcctgcatag taaaccgcaa
     8041 cgcccctgag agtgagtatc atttataatg ataattcaaa ctatcacata aataagattt
     8101 atatataatt tatattattc aggcaatgaa ttacttttgc aagccatcgc attctcttat
     8161 gttattaatg agttatgctg atttgttaag cagttttatc acgcttgaaa tggcgtcccg
     8221 ccccgacagg tgaatcgtcg gggctgattt tttcttatta ttgcagcaga agcttctcga
     8281 ggaactggcg ggtgcgaggc tgctggggat cggcaaataa cgcttttgct gccccctgct
     8341 cgactatccg cccctggtcc ataaagatcg cccggtccgc aacatcccgg gcaaagctca
     8401 tttcgtgtgt cacaatcacc atcgtgcgct tttcctgcgc cagctggcgg atggtgttca
     8461 ggacttcgcc caccagctct ggatccagcg ccgacgttgg ctcgtcaaac aaaatcacct
     8521 caggacgcat tgccagtgca cgcgcaatcg caacacgctg ctgttgaccg ccagacaaac
     8581 gacgtggata gctggtttct ttaccggcca gcccaacttt cgccagcagc tcgcgagcgc
     8641 gcgccgtggc ctcttctttc ggttcgcctt tgacgatcac cggcccttca ataatgtttt
     8701 ccagcaccgt acgatgcgga aacaaattaa agttctggaa gacaaacccg acgtgctgac
     8761 gcaactggcg aatcagcgat ttttgctgac ttaatgaacg tgcagtatca atagtgatat
     8821 cgccaacggt gatcgttccc gcttccggtt gttccagcag atttatgcta cgtagcaatg
     8881 tggttttgcc ggaaccactc ggaccgataa ttgccaccac ttcgccaggc tttacctcga
     8941 ggtcgatacc gtgcagcacc gtctgaccgt ggaatttttt caccaggttc ttaacttcaa
     9001 tggcactcat tttggttctc tctcctggcg attaagttga ttctcaaaat ggttctgcaa
     9061 cgtcgataac acagtcgcca tgatccagta gatgagcgaa gccgccaggt acatggtgaa
     9121 cacctccagc gtgcgcgagg taatcaactg cgcctgacgg aacagctccg gcacctggat
     9181 tgtcgcggcc agcgacgtat cttttaccag actgataaag ctgttcgaca gcggcggcaa
     9241 cgccacccgc gccgcctgtg gcaaaatcgc gcgacgcatg gtctgccacg gcgtcatacc
     9301 aatactggca gcggcttccc actgaccttt atcaatagaa gaaatggcgg cgcgcagcgt
     9361 ttcggcggca taggcggcag tgttcagcga cagaccaatc attgctgacg gaatgggatc
     9421 taattcgata ccaaactgcg gcaggccgta atagatcata aacagttgtg cgatgagcgg
     9481 cgtaccacga aaaatagaga tataaaaacg cgccagccag cgcacgggcc agattggcga
     9541 caggcgcatc agcgcgagaa taaacccgag tagtaagcca aaaaacatgc cgccgatact
     9601 gagttgcagg gtatacccag cccctttcaa caggaacggc aaggaatcaa taaccagttg
     9661 tatactttct tgcatgagcg ttcccggagc taaacgtggg gatgataggc gaacagcgca
     9721 ggcgcgccgc cggtatgaat aaacagaatc ggcccttcat ctttgaagcg tttctgactg
     9781 ataccgtcaa tcagccccgc catcgctttt ccggtataca caggatcaag cagaatgcct
     9841 tccagacgcg ccagcaattt cactgcttcc atgccttcgt cgttcggcac gccgtagcca
     9901 ggtgcaaaat agtcatccca gagtaaaatt tccgctgatg cggtcagctc cagttctttc
     9961 gcaatcgcct gttgtaggtt aaccactttc ggcaattgat cggcaacgga acgcgacacg
    10021 gtcacgccaa tcagttcgct ttcaggcatc aggtgttcca gcccaacagc cagtccggcg
    10081 tgagttccgg cactgcccga tgcgactacc accgacgaaa tattaaccgc cccttcacac
    10141 tgttgcgcga tttccagcgc actctccaca taacctagcg cgccaagagc attagaaccg
    10201 ccaaccggaa tgacatacgg gcgaaagcct tgtgcttcga ctcgcgtcgc cagttcttcc
    10261 agttgggcat tgggatcggt cagtgcgtcg cacatttcaa tctgggtatt gaacagatcc
    10321 agcaacaaac gattgccgtt ggttaaatag ttttctgcgg ttgtgccaat aggattttcc
    10381 agcagcgcca cgcagtgcag accgagtttc gccgcgactg cggcagtctg gcgcacatgg
    10441 ttagactgga tcgcgccggc agtaatcagc gtatcggcac cttcgcgcag ggcatctgcc
    10501 gcgagaaatt ccagcttacg taatttattg ccgcccattg ccatgggggt gacctcatcc
    10561 cgtttgatga aaatttcccg tccaagataa tcagaaaagc gcggcagata ttcgagcggc
    10621 gttggcgcgc cgataaactc caaccgtgga aaacgggtta aattatgcag tggcataaca
    10681 gcctccgatg tgtgttgttg tgattttctt attatgcacg ctgaaaacgc gtaaataaaa
    10741 aaggcgctag tgaaagcgcc cttttttgtc attatgctga ttatttggtc acatcagcac
    10801 caaaccattt ttcggaaaga gcttgcagag tgccgtcttt ttgcatttcc gcaattgcat
    10861 cattcaccgc tttcagcagg tcttcatttc ctttacgcag cgccacgcca gactcctgac
    10921 gggagaatgc ttcaccggtt actgccagcg tatcgttggt tttcttcacc agatccagcg
    10981 ccgccagacg atcaacgagg atcgcatcga tacgccctac gcgcagatcc tgatatttgg
    11041 tcgggtcatc atcataggta cgcacatcga caccctgaac attctgccgc agccactctt
    11101 catagttggt gcccagaccg acacccactt ttttgccttt cagatcagca gccgttttaa
    11161 tggtgccttc gttacctttt ttcaccagcg cctgaatacc ggaaatggtg taaggggtag
    11221 agaaatcgta ttttttcttg cgctcgtcag aaatggtgac ctgattaatc accacatcaa
    11281 tgcgtttaga gtccagcgac gccagcatac cgtcccattt ggtcggtttt agtgacgcct
    11341 caacgccaag atgttttgcc agctgttggg caaattccac ttcaaaaccg gttaatttgc
    11401 cgtcatcccc ctgaaaactg aacggcggat aagtgccttc cagccctacc agcagcgtgc
    11461 cgctctcttt gactttatta agcagacctt catctgcaaa acttttaacg ctcatgcccg
    11521 caaccagcgc cacggccatc acacccatca atgcctgacg tcccagatgt gctaatttca
    11581 tattcacccc gaatgttgtt atgtctgttt gcagtgtaga gccattgttt gtaaacacaa
    11641 aaacaactcc gctacatctt attcttattt aatatatatc agaagcaggc aggctggagg
    11701 atttctgcac aagtccggta caggtttggc gctgatagtg ttgatacttc cgtaatgcga
    11761 tgcggtaatt gttggtgctg gtggtgggca gccacggggc gaggatttca tcaaggaaac
    11821 cgtcctgcag ctgatcgagg gaaatatttt gctcatgtag atgattaccc aaacggcgca
    11881 gacgaacgac atattcacgc accgtacctg ggctcatttc agtttgctca aaaagaaatt
    11941 tcttaaagcc gataatatcg aaaaaatcac tctgcgtttt gcaatgtaaa tcaccgcaaa
    12001 atcggcacaa tgccgcccat gattgttgtt ccgtttgcca gcctttttca tcaagcagcg
    12061 tgtccaggcg ggaaatctcg attttattca ctattttgcc gtcgcgaact aaggtaatgc
    12121 gatcgagtaa tttacggcaa tgcgcgcaat gggtctggct gtgtttaaag tctttaagat
    12181 agcggcttaa tggccgtctt ttcaggtgct gcaccatcat taagaactcc tggtagtcaa
    12241 agttaaagtg cggcatttac tgacgttata acttacccag tttagtgcgt aaccgtttaa
    12301 tgtcctggct gtgtaactga ctgacccgcg attccccgac ctccagcacc gcgccaatct
    12361 ctttgagatt cagctcttcc tggtaataga gggttaatac cagtttttcg cgctccggca
    12421 acgtttcgat ggcttccatc acccgctggc gcagattact gtccagcagt tgttgtagcg
    12481 ggttttctcg ctgatgatca tcagtaacca gttcgatgct atcgccgtgc tcttcacgcc
    12541 actcatcgta ggagaagagc tggctgttat tggtgtcgag cagcatttga cgataatcgg
    12601 caatatcgat ccctaaacgt tccgctacct cagtttccgt ggcgttgcgg ccaagttcct
    12661 gctccagttg ccctattgcc tgtgccactt cacgcgcgtt gcgtcgcacg ctgcgcggca
    12721 cccagtcacg gctgcgaagt tcatccagca tagcgccacg gatacgctac actgcgtaag
    12781 ttgtaaatgc cgttccttgt agggcgtcat agcgttcgac agcattaagt aacccaatgc
    12841 cgcccgcctg tagcagatcg tcaagttcca cgctcgcggg cagtcgaacc tgcaggcgca
    12901 atgcttcgtg acgcaccagc gggacataac gctgccacag cgagtgttta tccattacac
    12961 cttcagcggt atagagtgaa ttcacgataa acagccctgc gttatatgag ttatcggcat
    13021 gattatccgt ttctgcaggg tttttaatcg gacgattagt gggtgaaatg aggggttatt
    13081 tgggggttac gcaaaaagcc gaatgtaaaa aaccccgccg gtggcggggt ttgagaggca
    13141 ttcagacgat taaccctgca gcagagacag aacctgctgc ggtacctggt tggcttttgc
    13201 cagcacggag ttaccggcct gctggataat ctgcgctttc gacatgttgg acacttcggt
    13261 cgcatagtcg gcgtcctgaa tacgggactg cgcttcagac aggttggtag tggtgttgtt
    13321 caggttggtg actgcggaat ccaaacggtt ttgcaccgca cccagggagg agcggaattt
    13381 gtcgatggag ctgatggctt catccagagc tttcagggga tcggctgtcg tagctgcttt
    13441 tgtttcagca tctgtcgtca atttaccatc agcatcttta taaactgcct taccgctgcc
    13501 gttagtcact gaaccatcat cctgtaggta aaaatctaca gtatcagatg tggtcaacgc
    13561 ctcgttgtta tcaacataaa ccgaatcaac accttttgtt acagcgccag ttgtgccgtt
    13621 gacgctataa cctgcagtag tgaaatcaac attgtttccc gaggttgata cagctttatc
    13681 gaaagctgcc tgatcaactt ttgcacctgt tactgaataa gtaatagtgt ccggcgtagt
    13741 actattaccg gtaaaggaga tagaggtacc gccaatgtca atggttgtcg ctttggtatt
    13801 attggctgca agtgtggaca gactagcctc agtcaaagaa gcaccagagc cgtttttcgt
    13861 taagcgccca gttgtatcaa tgtagagttt atctccattg cttgccgtaa ttttaccatc
    13921 actgctgatc tgaacatcct gtgcagaacc atcgatagta atagtgccct tagcggtatc
    13981 accaactccc ggggtcaaat atttctgaac atcggcagct gaagccgttg tggtatcaaa
    14041 agagtatgac ttacttgcac tgtcatactt gtaattcgtt gcactagccg ccgttccgaa
    14101 gccgttatta atggttgcct gaacagtatc accatcacta actccagcca gcaaatcaga
    14161 cgctttagca gcatcgtaac ccgcactcac agtatatttg ttgccgacca ctgttgcatt
    14221 agcagctacc aagtcagctt tagatgcagc agtattagcc acagccccgc caccattcac
    14281 attaaaccca ttcagcccca gcgtatcaga gtcaattttc ttcagatcaa tagtgatagt
    14341 ctggccgtca ttcgcaccaa cctgaatttt catcgaaccg tcttttgcca gtacgttcac
    14401 gccgttgaac tgggtctgac cggatacgcg gtcaatttcg tcgagacggg atttgatttc
    14461 gtcctgaatg gagtccagat ccgaatcaga gttagtcccg gtagaagcct gaaccgtcag
    14521 ctcacggata cgctgtaagt tgttgttgat ttcggacagc gcgccttcag tggtctgtgc
    14581 aacagaaata ccgtcgttgg cgttacgtgc agcctgagtc aggcctttaa tgttagaagt
    14641 aaaacggtta gcaatcgcct gacccgcggc gtcatccttc gcgctgttaa tacgcaagcc
    14701 agaagacaga cgctcgatag aactcgacag cgcagactgg ttcttgttga tattattttg
    14761 agtgatcagc gagaggctgt tggtattaat gacttgtgcc atgattcgtt atcctatatt
    14821 gcaagtcgtt gattacgttt tgggtttcca cccgtcggct caatcgccgt caaccctgtt
    14881 atcgtctgtc gtaaaacaac ctttagaatt tttttcacaa acagccattt tttgttagtc
    14941 gacgaaatac tcttttctct gccccttatt cccgctatta aaaaaaacaa ttaaacgtaa
    15001 actttgcgca attcaggccg ataaccccgg tattcgtttt acgtgtcgaa agataaaagg
    15061 aaatcgcatg gcaagtattt catcgctggg agtcgggtca ggtctggatt taagttcaat
    15121 ccttgatagc ctcaccgccg cgcaaaaagc gacgctaacc cctatttcaa atcagcaatc
    15181 gtcgtttacc gctaaactta gcgcctacgg tacgctgaaa agcgcgctga cgactttcca
    15241 gaccgccaat actgcattgt ctaaagccga tcttttttcc gccaccagca ccaccagcag
    15301 caccaccgcg ttcagtgcca ccaccgcggg taacgccatc gccgggaaat acaccatcag
    15361 cgtcacccat ctggcgcagg cgcaaaccct gaccacgcgc accaccagag atgatacgaa
    15421 aacggcgatc gccaccagcg acagcaaact caccattcaa caaggcgacg acaaagatcc
    15481 gattaccatt gatatcagcg cggctaactc atcgttaagc gggatccgtg atgccatcaa
    15541 caacgcaaaa gcgggcgtaa gtgcgagcat cattaacgtg ggtaacggtg aatatcgtct
    15601 gtcagtcaca tcaaatgaca ccggccttga taatgcgatg acactctcgg tcagcggtga
    15661 tgatgcgcta caaagtttta tgggctatga cgccagtgcc agcagcaacg gtatggaggt
    15721 ctcggtcgcc gcccagaatg cgcagctgac ggtcaacaac gtcgccatcg agaacagcag
    15781 caacactatc agcgacgcgc tggaaaacat cactctgaac ctgaacgatg tcaccacggg
    15841 caaccagacg ctaaccatca ctcaggacac ctccaaagtg caaacggcga ttaaagactg
    15901 ggtgaatgcc tataactcgc taatagatac cttcagcagc ctgaccaaat acaccgccgt
    15961 agatgcggga gctgatagcc agagttctag caatggcgca ctgctcggcg actccacgct
    16021 gcggacgatt cagacgcagt tgaagtcgat gctgagtaat accgtcagtt cttccagcta
    16081 taaaacgctg gcgcagattg gtatcacgac cgatcccagc gatggcaaac tggaactgga
    16141 tgccgacaaa ctcaccgctg cactgaaaaa agatgccagc ggcgtaggtg cattgattgt
    16201 tggcgatggt aaaaaaaccg gcatcacaac caccatcggc agcaacctga ccagttggct
    16261 ttcgacaacg ggcattatta aagccgctac cgatggcgtt agtaaaaccc tgaataagtt
    16321 aactaaagac tacaacgccg ccagcgatcg cattgatgcg caggtcgcgc gctacaaaga
    16381 acaatttacc caactggacg ttttaatgac ctcgttaaac agcaccagca gctacttaac
    16441 gcagcagttc gaaaacaaca gtaattccaa gtaatcatta ttcatcggga gacaggtcat
    16501 gtacgcggca aaaggcaccc aggcctatgc acaaattggc gtcgaaagca ccgtaatgag
    16561 cgccagccaa cagcagttgg tcaccatgct atttgatgga gtgctgagcg cactggttag
    16621 agcgcgactg tttatgcagg acaacaatca gcaaggcaaa ggcgtctctt tgtcaaaagc
    16681 gatcaacatc attgagaacg gactgcgggt gagtcttgat gaagagagca aagacgaatt
    16741 aacccaaaac ttaattgctc tttatagcta tatggtcagg cgtttgctgc aagccaattt
    16801 acgcaacgat gtctcagcag tcgaagaggt ggaagcatta atgcgtaata ttgccgatgc
    16861 ctggaaagag tctttactct ccccttcttc gattcaggac ccagtctgat gaacaatgca
    16921 ccgcatttgt atttcgcctg gcaacaactc gtcgaaaaaa gccagctcat gttacgcctg
    16981 gcaacggaag aacaatggga cgaactcatc gctagcgaaa tggcgtatgt gaatgcggtg
    17041 caggagatcg cacacctgac tgaagaggtt gccccgtcca ctacgatgca ggagcagctc
    17101 cgcccgatgc tgcacctgat tctcgacaac gaaagcaagg taaagcagtt attacagatt
    17161 cggatggatg aactggcgaa actggtcggt cagtcatcgg tgcaaaaatc ggtgttaagt
    17221 gcctatggcg atcagggcgg ctttgtgctg gctccgcagg ataacctctt ttgaatctta
    17281 atgagccgat ggctcgcgaa taatccgatt acggctacgc ttctaatatt ccccttgaat
    17341 ggagtcgaag aatgcgtaat cccacgctgt tacaatgttt tcactggtat tacccggaag
    17401 gcggtaagct ctggcctgaa ctggccgagc gcgcagacgg ttttaatgat attggtatca
    17461 atatggtctg gttgccgccc gcctataaag gcgcatcggg cggctattcg gtcggctacg
    17521 actcctatga tttatttgat ctaggtgagt ttgatcagaa aggcagcatc cctactaaat
    17581 atggtgataa agcacaactg ctggccgcca ttgacgccct gaaacgtaat gacattgcgg
    17641 tgctgctgga cgtggtagtc aaccacaaaa tgggcgcgga tgaaaaagaa gctattcgcg
    17701 tgcagcgtgt aaatgctgat gaccgtacgc aaattgacga agaaatcatt gagtgtgaag
    17761 gctggacgcg ttacaccttc cccgctcgtg ccgggcaata ctcgcagttt atctgggatt
    17821 tcaaatgttt tagcggtatc gaccatatcg aacaccctga tgaagatggc atttttaaaa
    17881 ttgttaacga ctataccgga gaaggctgga acgatcaggt tgatgatgaa ctcggtaatt
    17941 tcgattatct gatgggtgag aatatcgatt ttcgcaatca tgccgtgacg gaagagatta
    18001 aatactgggc gcgctgggtg atggaacaaa cgcaatgcga cggctttcgt cttgatgcgg
    18061 tcaaacatat tccggcatgg ttttataaag agtggatcga acacgtacag gaagttgcgc
    18121 caaagccgct gtttattgtg gctgaatgct ggtcgcatga agtcgataaa ctgcaaacgt
    18181 atattgatca ggtggaaggc aaaaccatgc tgtttgatgc gccgctgcag atgaaattcc
    18241 atgaagcatc gcgcatgggt cgcgactacg acatgacgca gatcttcact gggacgctgg
    18301 tggaagccga tccttttcac gccgtaaccc tcgttgccaa ccacgacacc caaccgttgc
    18361 aagccctcga agcaccggtc gaaccgtggt ttaaaccgct ggcgtatgcc ttaattttgt
    18421 tgcgggaaaa tggcgttcct tcggtgttct atccggacct ctacggtgca cattacgaag
    18481 atgtcggtgg tgacgggcaa acctatccga tagatatgcc aataatcgaa cagcttgatg
    18541 agttaattct cgcccgtcag cgtttcgccc acggtgtaca gacattattt ttcgaccatc
    18601 cgaactgcat tgcctttagc cgcagtggca ccgacgaata tcccggctgc gtggtggtca
    18661 tgtcgaacgg agatgatggc gaaaaaacca ttcatctggg agagaattac ggcaataaaa
    18721 cctggcgtga ttttctgggg aaccggcaag agagcgtagt gacagacgaa aacggcgaag
    18781 cgaccttctt ttgcaacggc ggcagcgtca gcgtgtgggt tatcgaagag gtgatttaaa
    18841 tccatccccg gcggcaagcc ggggagattt cattacggca gtggcgtcgg taacggcgct
    18901 ttatccagcg cggcggcaca ctctgccgtc gggcgatcca cacgctccat cgtcatacca
    18961 tcgtactcaa tggtgttacc gtcacgctca acctcgtaca gctcacgttt tacggtaacg
    19021 ttagtgagat catcagacat caatgtcaac ttacccggca cggcaatccc ccgctgccac
    19081 tgacggcaat ccaaggtatc gccctcttta gtgacaatca ggctgcctat cgcttccgga
    19141 ctgaccagcg cacgttgcgg ccctttggtc tgccagtagc ccgccagcca gtccggcgcg
    19201 ggggttttca cgacattgtt atagttttcg acttcggcac agcccgccag caacatgagc
    19261 gcgcctgcaa ttgcgagttt tttcatcatt cgtcctgcat gagaagaaaa gaggattgtg
    19321 gcattaaagc tctgatgtcg ccagcctttc caggttcaga gagaaatttg atctaactat
    19381 tatttttgtg aattattacc ccgaaaataa atttgcatgg tgtatgattc gcggctcttg
    19441 tttcaaacct tctgagttca gaggctacat tcatgtcatg gcagcaattc aaacacgcct
    19501 ggttgattaa attctgggca cccatccctg cggtcatcgc ggcgggtatt ctctccactt
    19561 actattttgg cattactggc accttttggg ctgtcacggg tgaatttacc cgttggggcg
    19621 gtcagctcct gcagctgttt ggcgtccatg ctgaagagtg gggttatttt aaaattatcc
    19681 atctggaagg atcgccatta acccggatcg acggcatgat gatcctcggt atgtttggcg
    19741 gctgctttgc tgctgcgctg tgggccaaca atgtcaaact acgcatgcca cgcagccgta
    19801 tccgcattat gcaggccatc attggcggca ttatcgccgg ttttggcgcg cgtctggcaa
    19861 tgggctgtaa cctggcggcg ttctttaccg gcattcctca gttctcgctg catgcctggt
    19921 tctttgccat cgccactgcc attggttcat ggtttggcgc gcgctttacc ctgctgccca
    19981 tcttccgtat tcccgtgaaa atgcagaaag tttctgccgc ctcaccgctg acgcaaaaac
    20041 cggatcaggc gcggcgtcgt tttcgtctcg ggatgctggt ctttttcggc atgctgggct
    20101 gggcgctgct cacggcgatg aaccaaccaa aactggggct ggcaatgctg tttggcgtcg
    20161 gctttggttt actgattgaa cgtgcgcaaa tctgctttac ttcggcgttc cgcgatatgt
    20221 ggatcaccgg acgtacccat atggcgaaag caatcattat cggtatggcg gtgagtgcca
    20281 tcgggatctt cagttacgta cagttaggcg ttgaacctaa aatcatgtgg gcgggaccaa
    20341 atgcggtaat tggtggttta ctttttggtt ttggcatcgt gctggcaggc ggctgcgaaa
    20401 ccggctggat gtaccgcgcg gtagaaggcc aggtgcacta ctggtgggtc ggtctgggca
    20461 acgtgatcgg ctcaacgatt ctggcgtatt actgggatga tttcgctccg gcgctggcca
    20521 ccgactggga caaaatcaac ctgctgaaaa cttttggtcc gatgggaggc ctgctggtga
    20581 catatttgct gctgtttgct gcgctgatgt tgattatcgg ctgggaaaaa cgcttcttcc
    20641 gccgtgaggc accgcagact gctaaggaga tcgcatgaaa aatatcgttc ctgattaccg
    20701 ccttgatatg gtgggtgaac cctgccctta tccggcagtc gcaacccttg aggcgatgcc
    20761 gcaattaaaa aaaggtgaga tcctggaagt ggtgagcgac tgtccgcagt cgatcaataa
    20821 tattccactg gatgcgcgta atcacggcta cacggtgctg gatattcagc aagacgggcc
    20881 gaccattcgt tatttaattc agaagtaatc cctcattccc gctgtggccc acagcgggaa
    20941 catcgttacc tctgacacat agattttacc cgccccctct cctattcttt tcagtatgat
    21001 ctcaggaggt tgctatgtgt ggacgctttg cccaatccca aacgcgtgag gattaccttg
    21061 cgcttctcgc ggaagatatt gaacgcgata ttccctacga tcccgaaccc attggcagat
    21121 acaatgtcgc gccgggaacc aaagttcttc tgctcagtga acgtgatgaa caccttcatc
    21181 tggatccggt tttctgggga tatgctcccg gatggtggga taaaccgccg ctgattaacg
    21241 cccgcgtaga aactgcctca accagtcgca tgtttaaacc gctctggcaa catggtcggg
    21301 caatctgttt tgccgatggc tggtttgagt ggaaaaaaga aggcgacaaa aaaagcctta
    21361 ttttatctat cgcgctgacg gacaacctgt ttttatggcc gcgataggta gcataccgtt
    21421 tgagcgtggt gacgaagccg aaggattttt gatcgtcact gcggcggcag atcaaggtct
    21481 ggtagatatt catgaccgcc gcccgctggt tctgtcgcca gaagctgcac gggaatggat
    21541 gcggcaagac attggtggta aagaagcctc agaaatcgct accagaagct gtgtgccagc
    21601 aaaccagttc acctggcacc ctgtgtcgcg cacggtgggt aatgtcaaaa accagggcgc
    21661 ggagttaatt caacctgttt gttgacatcc tccacgccct gaatgacgag gacccctgct
    21721 acgttcaggc tgtcgcctga atcatttcgg tgggttcctg cttcaacgag cggcctgact
    21781 gcaccatctc tccacaggca agaacggcgt gccccgccgc taaaatgtta cgagctccat
    21841 ttacatcggc gttcgctgta tatccacata cctggcatct gaatttactt tgtgacaggc
    21901 gattttcttt cgctgtatga ccacagcacg cgcaacgctg gcttgtgtac gctggcggaa
    21961 cagcaagcac ctgaccgcca cgccagagct gcttgtactc aagctggcgg cgcatttcat
    22021 accagccctg atccagtatc gaacggttta aacctgattt t
//
LOCUS       DAZSIO010000056        21987 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZSIO010000056 DAZSIO010000000
VERSION     DAZSIO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21987
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.056"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            139..765
                     /locus_tag="ACHX5T_001949"
     CDS             139..765
                     /locus_tag="ACHX5T_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR8467169.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            786..2887
                     /locus_tag="ACHX5T_001950"
                     /pseudo
     CDS             786..2887
                     /locus_tag="ACHX5T_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            2900..3538
                     /locus_tag="ACHX5T_001951"
     CDS             2900..3538
                     /locus_tag="ACHX5T_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR8467170.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3602..4270
                     /locus_tag="ACHX5T_001952"
     CDS             3602..4270
                     /locus_tag="ACHX5T_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR8467171.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4267..5052
                     /gene="phsC"
                     /locus_tag="ACHX5T_001953"
     CDS             4267..5052
                     /gene="phsC"
                     /locus_tag="ACHX5T_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR8467172.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5056..5868
                     /gene="ydhT"
                     /locus_tag="ACHX5T_001954"
     CDS             5056..5868
                     /gene="ydhT"
                     /locus_tag="ACHX5T_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR8467173.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(5880..7302)
                     /locus_tag="ACHX5T_001955"
                     /pseudo
     CDS             complement(5880..7302)
                     /locus_tag="ACHX5T_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(7428..7733)
                     /locus_tag="ACHX5T_001956"
     CDS             complement(7428..7733)
                     /locus_tag="ACHX5T_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR8467174.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(7841..7917)
                     /locus_tag="ACHX5T_001957"
     tRNA            complement(7841..7917)
                     /locus_tag="ACHX5T_001957"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7881..7883),aa:Val,seq:gac)
     gene            complement(7922..7998)
                     /locus_tag="ACHX5T_001958"
     tRNA            complement(7922..7998)
                     /locus_tag="ACHX5T_001958"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7962..7964),aa:Val,seq:gac)
     gene            8306..9562
                     /locus_tag="ACHX5T_001959"
     CDS             8306..9562
                     /locus_tag="ACHX5T_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR8467175.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9603..10976)
                     /gene="mdtK"
                     /locus_tag="ACHX5T_001960"
     CDS             complement(9603..10976)
                     /gene="mdtK"
                     /locus_tag="ACHX5T_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR8467176.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            11191..11832
                     /gene="ribE"
                     /locus_tag="ACHX5T_001961"
     CDS             11191..11832
                     /gene="ribE"
                     /locus_tag="ACHX5T_001961"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR8467177.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(11872..13020)
                     /gene="cfa"
                     /locus_tag="ACHX5T_001962"
     CDS             complement(11872..13020)
                     /gene="cfa"
                     /locus_tag="ACHX5T_001962"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR8467178.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13311..14522)
                     /gene="punC"
                     /locus_tag="ACHX5T_001963"
     CDS             complement(13311..14522)
                     /gene="punC"
                     /locus_tag="ACHX5T_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR8467179.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            14635..15567
                     /gene="punR"
                     /locus_tag="ACHX5T_001964"
     CDS             14635..15567
                     /gene="punR"
                     /locus_tag="ACHX5T_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR8467180.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            complement(15564..16589)
                     /gene="purR"
                     /locus_tag="ACHX5T_001965"
     CDS             complement(15564..16589)
                     /gene="purR"
                     /locus_tag="ACHX5T_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR8467181.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            16888..16977
                     /gene="cydH"
                     /locus_tag="ACHX5T_001966"
                     /gene_synonym="ynhF"
     CDS             16888..16977
                     /gene="cydH"
                     /locus_tag="ACHX5T_001966"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR8467182.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            17143..18312
                     /locus_tag="ACHX5T_001967"
     CDS             17143..18312
                     /locus_tag="ACHX5T_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8467183.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18458..19039)
                     /gene="sodB"
                     /locus_tag="ACHX5T_001968"
     CDS             complement(18458..19039)
                     /gene="sodB"
                     /locus_tag="ACHX5T_001968"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR8467184.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19167..19994)
                     /gene="mepH"
                     /locus_tag="ACHX5T_001969"
     CDS             complement(19167..19994)
                     /gene="mepH"
                     /locus_tag="ACHX5T_001969"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR8467185.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20329..20676
                     /gene="grxD"
                     /locus_tag="ACHX5T_001970"
     CDS             20329..20676
                     /gene="grxD"
                     /locus_tag="ACHX5T_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR8467186.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20790..21200)
                     /gene="tnpA"
                     /locus_tag="ACHX5T_001971"
                     /pseudo
     CDS             complement(20790..21200)
                     /gene="tnpA"
                     /locus_tag="ACHX5T_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            21223..>21987
                     /locus_tag="ACHX5T_001972"
     CDS             21223..>21987
                     /locus_tag="ACHX5T_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR8467187.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTT"
BASE COUNT         5675 a         5157 c         5714 g         5441 t
ORIGIN      
        1 gttaaatctt ttctcataca ctatcgggtt agggaaaata tctggtgttg gggacagagt
       61 cagcagcgta ttagttgaat gcttaatatc aggctgctga attgtcagca ctgtgtttgc
      121 gaagaggatg cgaaataaat gaacccggtt gatcgtccac tattagatat tggcttaacg
      181 cgattagaat tcttgcgtat atcaggaaaa ggccttgctg gtctaacaat tgcccctgcg
      241 ttactgtcac ttttgggttg caaacaggaa gatattgata gcggcacagt agggttgata
      301 aatacaccca aaggggtgct ggtgacccag cgagcgcgat gcacgggttg ccaccgctgt
      361 gaaatctctt gtaccaactt caacgatggc tcagtaggga cattcttctc ccgtatcaaa
      421 atccatcgca attatttctt tggcgacaac ggggttggct ctggcggcgg cctgtatggc
      481 gatctcaact ataccgcgga cacctgccgt caatgcaaag agccgcaatg catgaacgtc
      541 tgcccgattg gtgcgattac ctggcagcag aaagaaggct gtattaccgt cgatcataaa
      601 cgttgtattg gctgtagcgc ctgtactacg gcatgcccgt ggatgatggc caccgtaaat
      661 accgaaagta aaaaatcctc gaaatgtgtg ttatgcggtg aatgcgcaaa cgcctgccca
      721 acaggggcgt taaaaattat cgagtggaaa gatattactg tgtgaatctt gcaaaggaaa
      781 aaatcatggc taacggttgg acaggtaata tattaagagt caatctcacg acaggaaata
      841 ttaccctcga agattccagt aagtttaaaa gttttgtcgg tggcatgggc ttcggctaca
      901 aaattatgta tgacgaagta ccgctaggca cgaaaccttt cgatgaagcg aataaattag
      961 tctttgctac cggcccatta cctggatctg gtgccccctg tagttctcgc gtaaatatca
     1021 cctcactttc tacttttacc aaaggaaatt tagtcgcgat gcccatatgg gtggcttttt
     1081 tgcagcgcaa atgaaattcg ctggatacga cgtcattatt atcgaaggga aggcgaaatc
     1141 accggtatgg ctgaagatta aagatgacaa agttagcctg gaaaaagccg atttcttatg
     1201 gggaaaaggg acgcgcgcaa cgacggaaga aatttgtcga ttgaccagcc cggaaacctg
     1261 tgtggcggct attggtcagg ctggggaaaa ccttattcct ctctctggca tgttgaatag
     1321 ccgtaaccac agcggcggtg cgggaactgg cgcaataatg ggttcgaaaa acctgaaagc
     1381 gattgcggtt gaagggacga aaggggtcaa cattgccgat cgtcaggaga tgaagcgtct
     1441 caatgattac atgatgactg aacttattgg tgcgaataac aaccatgtcg tgccaagtac
     1501 gccacaatcg tgggcagagt attcagatcc caagtcacgc tggacagcac gtaaagggct
     1561 gttttggggc gcggctgaag gtggtccgat tgaaacgggt gaaattccgc caggcaatca
     1621 gaatacggtc ggctttcgta cctataaatc cgtttttgac ttaggaccgg cggcagagaa
     1681 atacacagta aaaatgagcg gctgccactc ttgcccgatc cgttgtatga cccaaatgaa
     1741 tattcctcgg gtgaaagagt ttggcgtgcc cagcacaggt ggtaacactt gtgtagcaaa
     1801 ctttgtccat accaccatct tcccgaacgg gccgaaagat tttgaagata aagacgatgg
     1861 tcgtgtgatt ggtaacctgg ttggtctgaa tttgttcgat gactacggcc tatggtgtaa
     1921 ctacgggcag ttgcatcgcg actttactta ttgttacagc aaaggtgtgt tcaagcgtgt
     1981 tctgccagct gaagagtatg cagaaattcg ctgggatcaa ctggaagcgg gtgacgttaa
     2041 cttcattaaa gatttttact accgtctggc gcatcgtgtg ggtgagctga gtcacctggc
     2101 tgatggttca tatgccatcg cagaacgctg gaatttgggt aaagagtact ggggctacgc
     2161 gaaaaataaa ctctggtcgc cgtttggcta tccggttcac catgccaatg aagcgtcagc
     2221 gcaggtcggt tccattgtta agtgtatgtt caaccgtgac tgcatgacgc atacccatat
     2281 caactttatt ggttccggct tgccattgaa actgcaacgt gaagtggcga aagaactttt
     2341 tggttctgaa gatgcttacg atgaaaccaa aaactacacg ccaatcaatg acgcaaaaat
     2401 taaatatgca aagtggtcgc tgttgcgggt ctgtttgcat aacgccgtca ctctgtgcaa
     2461 ctgggtctgg ccaatgaccg tttcgccgct gaaaagccgt aattaccggg gcgatctggc
     2521 gcttgaagcc aaattcttca aagcgatcac cggcgaagag atgactcagg aaaaattaga
     2581 tttagctgca gagcgtatct ttacgttgca tcgtgcctac acggtaaaac cgatgcaaac
     2641 caaagatatg cgtaatgaac acgatcttat ctgttcctgg gttttcgaca aggatccgca
     2701 gatcccggtc tttactgaag gtactgacaa aatggatcgt gacgatatgc atgcttcgct
     2761 cacgatgttc tacaaagaaa tgggctggga cccacagctt ggttgtccaa cccgcgaaac
     2821 attgcagcgt ctggggctgg aagatattgc cgccgatctg gcagcacaca atctactgcc
     2881 tgcgtaagga atgggtaaga tgaaccatca ggatgaattg ccgttagcga aagtcagcga
     2941 agttgacgag gcgaagcggc aatggttgca ggggatgcgt caccccgttg atacggtaac
     3001 cgaaccagaa cctgctgaaa tcctggctga gttcattcgc cagcattctg cggctgggca
     3061 gctggtggcg cgcaccgtgt ttctctcgcc accctattcg gttgcagaag aggaactgtc
     3121 agttttgctg gaaagcatta agcagaacgg agactacgcg gatatcgctt gtatgacggg
     3181 aagtcaggat gattactatt actcaacgca ggccatgagt gaaaactacg cggcgatgtc
     3241 tttgcaagtt gtggagcagg acatttgtcg cgccattgca cacgccgttc gttttgagtg
     3301 ccagacgtat cctcgtcctt acaaagtcgc catgctgatg caagcgccat attacttcca
     3361 ggaagcgcag attgaagccg cgatcgccgc gatggatgtt gcacctgaat atgccgatat
     3421 acggcaggtg gagtcatcga cggcggtgtt gtacttgttt agcgagcgat ttatgaccta
     3481 tggcaaggct tatggcttat gcgaatggtt cgaggtagag cagtttcaga acccctgaaa
     3541 tttcagggag atgtcatgga tagggtggac agttgcagcc actgcattag gggataatca
     3601 gatgtccttc actcgacgca aatttgttct ggggatggga acagtaatct tttttactgg
     3661 ttctgcgtct tctctgttag cgaacacgag gcaagaaaag gaagttcggt atgccatgat
     3721 tcatgacgag tcacggtgca atggctgtaa tatttgcgcc cgcgcatgcc gtaaaactaa
     3781 ccacgtcccc gctcagggaa gccgtttatc gatagcacac attccagtca ccgataacga
     3841 caacgaaacg cagtatcact tttttcgcca gtcatgtcag cactgcgaag atgctccgtg
     3901 catcgacgta tgcccgacgg gggcatcgtg gcgtgatgag caggggatcg tgcgggtaga
     3961 aaaatcgcag tgcatcggct gcagttattg cattggcgct tgtccgtacc tggttcgcta
     4021 ccttaaccct gtcactaaag tggcagataa atgtgatttc tgcgctgagt cccgactggc
     4081 aaaaggcttt ccgcccattt gcgtcagcgc ctgtccggaa cacgcattga tctttggtcg
     4141 cgaagatagc ccagaaattc aggcgtggtt gcaggataat aaatactatc aatatcagct
     4201 acctggtgcc ggaaaaccgc atctgtatcg ccggttcggt caacatttga ttaaaaagga
     4261 aaatgtatga acccgtcgca acatgctgaa cagtttcaga gccagttagc gaactatgtg
     4321 ccacagttca ctcccgaatt ttggccggtg tggttgatca ttgccggagt attgctggtt
     4381 gggatgtggt tggtgctggg gctgcacgcc ttgcttcgtg ctcgtggcgt gaagaaatca
     4441 gccaccgatc atggtgagaa ggtttatctt tacagcaaag cggtcagatt atggcactgg
     4501 tcgaatgcgt tactctttgt attgttgctg gcaagtgggc tgataaatca ctttgcgatg
     4561 gtgggcgcaa ctgcggttaa aagtctggtt gcggtgcatg aagtttgcgg atttttgtta
     4621 ctggcatgct ggctcggctt tgtgctgatc aatgccgttg gggataatgg tcaccactat
     4681 cgcattcgtc gtcaggggtg gctggaacga gcggcaaaac aaacgcgatt ttatttgttt
     4741 ggcattatgc agggggaaga acatcctttc ccggcaacaa cacagtctaa atttaatccc
     4801 ttacagcagg tcgcctatgt tggtgtcatg tatggattgc tgccgttgtt actattgacg
     4861 gggctgttgt gtctctatcc gcaagccgtg ggagatgtgt ttcctggcgt aagatactgg
     4921 ttattgcagg cgcattttgc tctggcattt ataagcctct tttttatctt cggtcatctt
     4981 tatctttgca ccacggggcg tacgccacac gaaaccttta aaagcatggt cgatggctat
     5041 caccggcact aacgaatgat cattacacga gcagacctgc gagaatggcg tatcggagcg
     5101 gtgatgtatc gctggttcct gcgccatttc ccacgcggcg gtagttatgc cgatattcgt
     5161 catgcactga ttgaggaagg atatacggac tgggcggaga gtctggttga gtatgcgtgg
     5221 aaaaaatggc tggcggatga aaacttcgcg catcaggaag tttcctctat gcaaaaactg
     5281 gccacagacc ccggagagat accattttgc agtcagtttg cccgcagcga cgatcatgct
     5341 cgcatcggct gttgtgagga taacgcccga atcgcaaccg ctgggtatgc cgcgcaaatt
     5401 gccagtatgg gttatagcgt gcggattgga agtgtcggct ttaacagtca cattggcagc
     5461 tccggtgaac gcgcacgggt tgctgtaacc ggtaattctt cgcggataag cagcgctgga
     5521 gatagtagtc ggattgcgaa taccggaatg cgagtacgcg tttgtacatt gggtgaacgg
     5581 tgtcatgtcg ccagtaatgg tgacctggtg caaatcgcca gctttggcgc aaacgccaga
     5641 attgccaaca gcggcgacaa tgtacacatc attgccagcg gtgaaaattc aacggtagtg
     5701 agcacgggcg tggtcgactc aattattctc gggctagggg gaagcgccgc actggcatat
     5761 catgacggcg aaagagttcg cttcgccgtc gccattgaag gtgaaaacaa tattcgaacc
     5821 ggagtccgct atcgtcttaa tgaacagcat cagtttgttg agtgctgata gctttacctt
     5881 taatcaaacg aaagacgccg acgagcacgg gatgcaggct ttacgaccgc ccgagccatc
     5941 gcctcaccaa tttctgcaca tgccgtaagt ccctgaacga aaggctggtc gtgcatcaac
     6001 cagggcaacg cgccgaacgc tacgcgtcca cgaatatctt cgggttgctg taacgtataa
     6061 tcttcgccaa catcagggat ttcagatccc cgcttcacgt aaggccagta gtcgacgaat
     6121 agactctgaa gggattgccg cataattatc gatgaaaacc ctggcaaggc ctttactgaa
     6181 ccgtttatgg tcgtgttcat tcagatgagg aacaatttcc tgtacggctt catgcaggcg
     6241 cagaatgaca taacgccagg gaacagtatg tttttctcgt ttattgcgtt cgacttcctg
     6301 gagatttttt tctgcccagt caaatgggtc gcgttgtttg cgctcggcaa accaggcttg
     6361 agcaaaggaa tcgacattca ggctctctaa ggctatgcgt tggctccagt ctggatcagc
     6421 aaacttgatt tcctctacta tcaatctaaa tacccgatcc aaaaggccct cttcaccttt
     6481 ttgaatctca gcatttaatg cctgatcggt gacgatgtgt aagggctcgt agggaatagg
     6541 gcaatagaaa tcggcttcgg gtaaaatacc cgtgcgcgac atcaacgtaa tatttagctt
     6601 ttcacttgcg ttatcgcggt gaaaaacgac gtgttgttta tcatcttcaa tgaacgaacc
     6661 atgctgaata gccactgcca ttgccgcatc cagtccgctc aaggatgttc ccataatacc
     6721 cacgttacac gcatcgacct ttgcttccat caggcctgac cacgggctgg gaaaatacgt
     6781 tctggttgct tcttcttcat caggccagac gtgacccgtg gcaatcaccg ctaaatcaaa
     6841 cgtctcgctg ggtaaatcct gatttgtagc gagcatgacg ccagcatttg taatttgcag
     6901 atcggtaacc tggcatgatt cataaacagc cactgcaaat ttttgctgtc gtgcctggtc
     6961 tactaatcgt aaaaattgat cgcggaaata ttcgcccagc agaattcgcg gtaaaaactg
     7021 acgatcgtgc aaggtttctt ttttaacgcc ataacgctgg agatggctgg cttcttgctt
     7081 ttgtagccat tcgagatacg tacaattaat cggcggtatt tcaatactgg caatatttgc
     7141 cagcatcatt tttgagtttt cctcatcact gtatggcatc ccgacaccgg cctcgtcagc
     7201 ctgctcgaag atagaaatag aaagtggagt ttgttgctgt agaagcgaga ataaggtgta
     7261 gatccccgta ggcccggcac ccacaatagc aatttttttc attgttactt acctgtacgc
     7321 attctttcga atttagtgtt atcagataag tttggttgag atttttagaa tttgagaaat
     7381 ttcagacagg gatgaaaagg gcgctaaccg cccttgatta aattctgtca ggcgagtttt
     7441 gcctgattga tttgactaag tggctcattg acatcgaaaa ctttggcgac aacttcctca
     7501 acgccgagat ttttcagtcg ggcagtatgt ttttccagat aggcaagggc gcttttttca
     7561 tcagtgaaaa ggtagatccc accggcttcg tggttctttt cactttctgt ccataccttc
     7621 cacagaaaac caggttcctg attaatcgac tcagcaagtg gtttaagctg ctcagccatt
     7681 gcgtcgccaa atgggccatt aaaagcaaaa tgaagttgta acaaagttgc catagcgatt
     7741 ctccttctga taacgcatac gaagacagtg taaatctttt aacgtattgg atacagagaa
     7801 gaaacagagg gaaaaaggaa aagaccagat taagaaaatc tggtgcgtcc gagtggactc
     7861 gaaccaccga cccccaccat gtcaaggtgg tgctctaacc aactgagcta cggacgcagg
     7921 atggtgcgtt caattggact cgaaccaacg acccccacca tgtcaaggtg gtgctctaac
     7981 caactgagct atgaacgcaa cgttgtaggt gacaacgggg acgaatatta gcggcagagt
     8041 ggaaaggtgg caagaggcaa aacgtaattt tctgcgctat ttcgaccgtt tgcagagctt
     8101 ttaagcaaat tggctatatt ttgttgattt gcaagggcga tttttattca ggatcgcatt
     8161 tacatctgat acaacccaat aattcgtgta tcagagttta aacagagttg tttgccacca
     8221 tctcaaatct gtcgtggata aatcctccgt agcctttaca cttaggtagc aataaaggca
     8281 cacagatgca gcaaaggaat aaatcatggg atctgatgcg aaaaacttga tgagcgacgg
     8341 gaatgtgcaa attgttaaga ccggcgaggt ccttggcgcg acgcaactta ctgaaggcga
     8401 gttgattgtt gaagctggcg gaagagccga aaataccgtg gtcacggggg ctggctggtt
     8461 gaaagtggca accggtggga tcgccaaatg cacacagtac ggtaacaatg gcacgctatc
     8521 ggtcagcgac ggtgccattg ccacagatat tgttcagtcc gagggaggcg caattagtct
     8581 ctctacgctc gctacggtta atggccgcca tcccgaaggt gaattcagcg ttgatcaggg
     8641 ttatgcctgc ggtttgttgc tggaaaatgg cggtaacctg cgtgtactgg aaggacatcg
     8701 cgcggaaaaa atcattctcg atcaagaggg cggcctgttg gttaatggga caacctcagc
     8761 ggtcgtggta gatgaaggtg gtgaattgtt ggtgtatcca agtggagaag ccagcaattg
     8821 tgagattaat cagggcggcg tttttatgct ggccgggaaa gccagtgata cgttgcttgc
     8881 tggtggcacc atgaataatc tcggtggtga agactctgac actattgttg agaatggatc
     8941 catttatcgt ctggggacgg atggccttca gctttacagt tccggtaaga cgcaaaacct
     9001 gtcggtgaat gtgggggggc gggctgaagt gcatgccggt acgctggaaa atgcggtaat
     9061 acaaggtgga acagtgatcc tgttgtcacc caccagcgcg gacgaaaatt ttgtcgtaga
     9121 ggaagatcgc gcaccggttg aactgaccgg gagtgttgca ttactggacg gcgcttcaat
     9181 gattattggt tatggcgcag agctgcaaca atcaacgatt actgtacagc agggcggtgt
     9241 gttgattctc gacggcagta cggtaaaagg tgacagcgtc actttcattg ttggtaacat
     9301 caatctgaat ggcggaaaac tgtggctgat cactggtgcg gcaacgcatg tgcaactgaa
     9361 agtgaaacgc ctgcgcggag agggagcgat ttgcctgcaa accagtgcga aagaaatttc
     9421 acctgacttc atcaatgtga aaggggaagt taacggggat atacgcgttg agataacaga
     9481 tgccagtcgg caaactctgt gtaacgctct gaaattacag ccagacgaag acgggattgg
     9541 cgcaacgctc cagcctgcgt aaaaacacag gcgcaacccg tgaaggttgc gccttgtctt
     9601 tattagcggg acgctcgttg cagaatgatg gctgacggca tacgttgcag gaaccgcata
     9661 cgcagcatca tcataatggc tgccgatgtc aggccgataa taaagcctat ccagaagcct
     9721 gctggcccca taggttcaac gaccagatcg gtcagtgcca gaatatagcc gcttggcaag
     9781 cccagcaccc agtaagctgt aaaggtgata tagaaaatgg aacgcgtatc tttataacca
     9841 cgcaaaatcc cactgccaat cacctggatt gagtcagaaa tctgatatac cgccgccagc
     9901 aacatcaaat gcgcagccag cgttacaacc tcgggattgt cgttgtacaa cagggcgatt
     9961 tgctcccgca gtgaaaccgt gaaaatggcc gtcagggttg ccatacagac acccaccata
    10021 agcccggtcc gcgcagcggt ttgcgcatcc agcgttgagc cctgacccag acgataacct
    10081 acgcggatag ttaccgctgc cgccagcgac attggaagca caaacatcag tgaactaaag
    10141 ttcagggcaa tctggtgtcc tgcgacatca acaataccga gcggagacac taacagagcc
    10201 acgacggcaa acagtgtcac ttcaaagaac agtgccagcg caatcggcaa accgagttga
    10261 atcagtcgtt tcataaccgc aggctcgggc ttagcggtgc ctttttcgtt acgaatatcg
    10321 cgcatggagc gggcgcgttt aatgtaagaa accatggcaa ggaacatgac ccaatacacc
    10381 gccgcagtag ccacgccaca accaacgcca ccgagctcag gcataccgaa atgaccataa
    10441 ataaagatat agttcaccgg gatgttcacc agcaggccga taaagcccat taccataccc
    10501 ggcttggttt ttgccagacc ttcacattgg ttacgggcaa cctggaagaa cagatatccc
    10561 ggcgcgcccc acaacaacgc acgcagataa cccacggctt tgtccgccag agccggatcg
    10621 atgttttcca tgtagcggat aatgtaacct gcattccaca gcaccagcat aatgagaacg
    10681 gaaacaaaac ctgccagcca gaaaccttgt cgcacctgat gcgcaatgcg ctcacgtcga
    10741 ccggaaccat ttaattgcgc gataaccggc gttaatgcca gcagcagtcc gtgaccaaag
    10801 aggatcgccg gaagccagat agaagtaccg atagcgaccg ccgccatgtc ggtggcacta
    10861 tagccgcccg ccatcacggt atcgacaaaa cccatcgcag tttgggcgat ttgcgcgaga
    10921 atcaccggga ttgctaatgc taataacaga cgcgcttcac tgatatactt ctgcacgtga
    10981 acacctttta tttgtagtta tatgagagac taaaaagccg cctgaatggg cagcaaaaag
    11041 aagaagcagg ggaaattcag tctattgtag cgaggattta ttaattctcc agtgaaaaaa
    11101 tagccattct taccccttca ctggtaaggg cgttttccac ctgctattgt gctgaacaga
    11161 atgtcttaac tgatttcagg agttgtaagt atgtttacgg ggattgtaca gggcaccgca
    11221 aaactggtgt cgattgacga gaaaccaaat tttcgtacgc atgtggtgga gttacccgac
    11281 cacatgctgg acggcctgga aaccggtgct tccgtggcgc ataacggttg ctgcctgacc
    11341 gtgacggaaa ttaacggcaa ccatgtcagt tttgacctga tgaaagaaac gttacgcatt
    11401 accaatcttg gcgatttaaa agtgggggat tgggtaaacg ttgagcgtgc ggcgaaattc
    11461 agtgatgaaa ttggcggaca cttaatgtca ggtcatatta tgaccaccgc tgaagtggcg
    11521 aaaatattaa cctcggaaaa taatcgccag atctggttta aagtccagga tagtcagttg
    11581 atgaaatata tcctgtacaa aggatttatt ggcatcgacg gtattagcct gaccgtcggc
    11641 gaagtcacgc caacgcgttt ttgcgtccat ttaattccgg aaacactgga acgcacgact
    11701 cttgggaaga aaaaacttgg cgcacgcgtc aacattgaaa tcgatccaca aactcaggca
    11761 gtggtagata cggtagaacg tgtgctggcg gcacgagaaa atgccatgaa tcaaccaggt
    11821 acagaagcct gatctgaact cgcccccgaa gagggggcga tagaatagcc tttagcgagc
    11881 gactcgaagg ccgttttcaa caccgcgtga gaacaccacc tgccagagct gaatatcacg
    11941 ggcgcggaaa gcacctgcgc aggcattcag ataataggta aacatccgtt taaagcgttc
    12001 actgtagtta tccgcaattt ctggccatgc ggcgaggaat cgttcatacc acgccatcaa
    12061 cgtagtatcg taatcagcac cgaagttatg ccagtcttcc atcacaaagt ggggttcgct
    12121 ggattgagca atctggcgta cagagggcag gcaaccgttc ggaaaaatat atttattaat
    12181 ccagggatca acattcagat cggttttttt cgaaccgata gtatggagca ggaatatacc
    12241 ttccggtttc aaattacgat ccaccaccgc aaaataggta tcgtaatttt tcggtccgac
    12301 gtgctcgaac atccccacag aaacaatacg atcaaactgg tcgttcaggt cacgataatc
    12361 ttgcagcaaa atggtgacat ccaggccttt acagcgttcc tgagccattt tttgctgttc
    12421 ggcagaaatg gtgacgccga ccacgcttac gtcatagtta gatgccatgt agtgcgctag
    12481 tccgccccag ccgcagccaa tatccagtac gcgcatccct ggttttaact gcaatttttc
    12541 acaaatcatt ttgagcttcg cctgctgggc agattccaga ttatcggcat ctttccagta
    12601 agcgcaggaa tattgcatga agggatcaag catgcggctg aacaagtcat tacccaaatc
    12661 gtaatgctcc ttgccgacta tccaggcacg ttttttactc tgcagattga agagacgagc
    12721 gccggcaata cgcagcgtgt ctttgaaatg atgggggagt tggttctcga gacctgcgcg
    12781 taagactttg ctaaaaaaca tatccagtcg gtcacattcc caccagccat ccatataact
    12841 ttcgcctaac cccaaagagc cttcctgcag aacgcgttta aaaaaatcgg ggtttttcac
    12901 acgaatatcc gccggggcag aaccgttaat ggctataccg gcacggctaa gtaattcgtt
    12961 ggcgatacgg taccagttgt catccggtac actgacttct tctatacacg atgaactcat
    13021 agtttctcca tcactggtcg gtgatcagaa ccttaaaaca gcgtagacgc tttttgggct
    13081 ttgtgagaaa tctcgcagaa aaaaccgcga gtctataatc cgacgtagaa cagagggaaa
    13141 ggaggaaccc ttgccgaaat gaccatccat agtgagtcgg gaacgatcct gtccccgtaa
    13201 aaacataata tttatcgtag ctttaacaac tcgaaattag tataggccgc taatttcaat
    13261 catacaacaa aattttgtta gcagcctaag tataagtata tcgatataga tcagtgtgat
    13321 tcgctatgag cgacttcggc attgccatga ttcgggcagt caacttcttc acaacgttgc
    13381 atcatgtaac ccagcgcgac cagcactact gttgataaca tcacgctggt ggtggtgagc
    13441 aatggcgtgc tgatactaat aagccaggaa actaccagac ttgcgaggaa gcacagaccc
    13501 agttgcagag tgttctgcaa cgctgcggcg cgaccggttg catgtgggaa gggacgcagc
    13561 gcctgggcga caacaatagg gtagatcgcg ccattggcaa tcgccatcac acagaatggg
    13621 atcaggattt cgaccagcga cacatggcta atgaagcccg cagcccaggt cgcaatgacg
    13681 ctgacagcaa acagcaccag caaccacggt aataactgct tgccttgcca tttctgcagt
    13741 gcggcgcgac agccataacc accaatcaga aacgcgatag tttgcgggac ataacttaaa
    13801 ccaataactg ccgggctgta gcccatttca ctaaggatga acggtgaacc ggtcagccat
    13861 gcaaaaaaac tggctgaaca ggctgcgtat atcagcacgt tgccgcgata ggttttagaa
    13921 cgtagcaggt cggtaaaggt cagaccatcc tgactattgt tacgggcctt cgtcgtgggt
    13981 ttgagccaga aaataggcag aatcagcacc acggtaatgg caaacagggt ggcgaaaatc
    14041 gcctgccagg aaaaatggac cagcagccag cttcctaaca ggggagccag tgccggagac
    14101 agacccacca gcggcatgat ggtcgcaaaa atacggttga ctttctgtga aggataataa
    14161 tctgtcacta atgcttgcca gataaccgcc gcggcgcaga cccccacagc ctgtacaaaa
    14221 cgcaatacca gcagcgtggc ggcgttttct acccacagca tccccagact acctaacgca
    14281 aaaattgtca ggccgattaa taacaccggt ttacgaccat aacggtcgga gagtggcccc
    14341 cacagaagct gggctgcggc aaaaccggca aggaacagac taaggctggc actgacagca
    14401 gacgcaggcg tttgcaggtc ggcctgtatg gcggcgaaag caggcagata catatcggtt
    14461 gccagaaaac cgagtacgct caaacccgcc agccagacta aaaatctttt cccaggttgc
    14521 atgtatattc tctaaatctc tcaattaaac accgccgcaa agtgtaggga gtgaaattag
    14581 tgttgtgaaa cggtaatatt tgatgactgg tttcaaaaat tttgcaggca gactatgtgg
    14641 tcagaatatt cactcgaagt tgttgatgcg gtagcgcgta atggtagttt tagcgctgcg
    14701 gcacaggagc tgcatcgtgt tccttctgcg gtcagctata ccgtgcgtca gctggaagag
    14761 tggctggcgg taccgctctt tgaacgacgg caccgtgatg tggagctgac cgctgctgga
    14821 gcgtggtttc tcaaagaagg gcgctctgtt gtcaaaaaaa tgcagatcac ccgccagcaa
    14881 tgtcagcaga tagcgaacgg ctggcgcggt cagttagcta tcgcagtgga taatattgtc
    14941 aggccagaac gtacacggca gatgatcgtt gatttttatc gccattttga tgatgtcgaa
    15001 cttcttgtct ttcaggaagt gttcaacggt gtctgggatg cgctttccga cgggcgcgtg
    15061 gaactggcaa ttggcgcaac acgcgcgatt ccggtaggcg gtcgttatgc cttccgggat
    15121 atggggatgc taagctggag ttgcgttgtt gccagtcacc acccgctggc gttgatggat
    15181 ggcccgttca gcgatgatac gttgcgcaac tggccgtcgt tggtgcgcga agacacctcg
    15241 cgaacgctac ctaaacgtat tacctggttg ctggataacc aaaaaagact tgtcgtacct
    15301 gactgggaat catcggcaac ctgtatctcg gcaggattat gcatagggat ggtgccaaca
    15361 catttcgcca aaccgtggct taatgagggg aagtgggtag cacttgaact ggaaaacccc
    15421 ttcccggatt cggcatgttg tctgacatgg cagcaaaatg atatgtcgcc tgcgttaacc
    15481 tggctgcttg agtatctcag cgatagtgag acgctgaata aggagtggct gcgggagccg
    15541 gaagagactc ccgcaacggg tgattaacga cgatagtcgc ggaacgggcc gtcagccacg
    15601 gagcggcgtt caatcaaacg cggatgcact tcaatggact gcggttcttc acgtttgttg
    15661 acgatacgat ccaacagcat gttgaacgct gtttcaccca gcgaatcttt tggctgatgg
    15721 atcgtggtca gcgccggcgt aaaatagcgc gcgttgcgca cgttatcata accgatcagc
    15781 gaaacatcct gcgggacgcg caggcccatt tcatcagcag cacaaagtgc gcccattgcc
    15841 atgatatcgc caccacagaa gacggcagta gggcgatgcg gctgcgacag gatttgctgc
    15901 atggcgcgat aaccggattc aggttcaaag tcaccctgca caatccagct ttccggcacc
    15961 ttgatcatcg cttcttccat cgccttcata aaaccggcaa ggcggcctgc gccggtgtta
    16021 cgttccagcg ggccggggat gacgccgatt tcgcggtgac cgcgttcaat cagataacgc
    16081 ccggccatgt agccgccttc gaacgcgtta tcaatgaccg catcggtgaa gtcagctttt
    16141 gcttcacccc agtccatcac caccattggg atatggcgat actcttccag catcgccagc
    16201 aacggctctg ggtactcaga acacatcacc agcagaccat cgacgcgttt ttgcgccatc
    16261 atcgacagat aagcccgctg tttctcaaga ttgttccacg cattacccag aatcagggtg
    16321 taacctttct ggaagcaatt tttttcaact gcttcaatga tctcggcaaa ataggccgct
    16381 tcgctgctgg tcgccagcaa accgatagac ttggtgtggt taaccttcag gctacgcgcc
    16441 accgcgctag gggagtagtg taattcttta atcgctgccc acacggcgtt gcgcgtttct
    16501 tcagcgacga aacgtgtttt gttgatcacg tgtgacacag ttgtagtgga aacgtttgct
    16561 cgtttcgcta catcttttat tgttgccatt ccatttcact ccagacccta acttcagctc
    16621 ctgcaaaatc gcaaggtaaa cgtttgcctt tacacacctt acgcacaaca aaagtgttgc
    16681 ggtacgccgg aaaaacgaca cggagcgtca ggaaggggtc aatggccagt acgcaaatag
    16741 tgtaagcgtg gaattttggc cgatcttgac gaaaagggga agtggtaaaa acacttatca
    16801 ttataaatga cgggagattt tttcatcaca gtgtgtaaaa atgcgtaata tcactttttg
    16861 tgtggtaata aaaaaggaga aaacttgatg agcaccgacc ttaaattttc actggtaaca
    16921 acggttatcg tcctcggttt gatcgtagcc gtgggtttga ctgccgcgct gcactgattt
    16981 cctctcgagg gagcgcgttc tccctcgcat ctccttcctg tttgttagca aaatggcaaa
    17041 agtgttttct ctttttgtta ttttcatttt tttgtgattg ctgtcatgct ttcgccactt
    17101 taatgaatat cgcgtaatga cgcgaagagg tggagataag gcatgaaaat taactatccg
    17161 ttgctggcgc tggcgattgg cgcgtttggt atcgggacaa cggagttctc gccaatgggc
    17221 ttgttgcccg tcattgcgcg cggtgtggat gtctcgattc ccgctgccgg aatgttaatc
    17281 agtgcctatg cagttggcgt aatggtgggc gcgccgctga tgacgcttct actttctcat
    17341 cgtgcccgcc gcagtgcgtt gattttcctg atggcaattt tcacgctagg caacgttctt
    17401 tccgccatcg cgccggatta tatgaccctg atgctttcac gtattttgac cagcctgaat
    17461 cacggagcat tttttggttt gggttcagtc gtggccgcaa gcgtggtgcc aaaacataaa
    17521 caggccagcg cagttgccac tatgtttatg gggttaaccc tggcaaatat cggtggcgtg
    17581 ccggcggcga cctggttggg tgaaaccatc ggctggcgga tgtcatttct ggcaacggcg
    17641 gggctgggag tgatttcaat ggtaagtctg ttcttctcat tacctaaagg tggtacaggg
    17701 gcacgacctg aagtgaaaaa agagctggcg gtattaatgc gtccgcaggt gctgtctgca
    17761 ttgctgacga cggtactggg agctggtgca atgtttaccc tctacaccta tatctctccg
    17821 gtactgcaaa gtattaccca cgcaacaccg gtgttcgtca cggcaatgct ggtgctgatt
    17881 ggtgtcggat tctctatcgg taactatctc ggcggcaaac tggcagatcg ttcagttaac
    17941 ggcacgttga aaggcttttt gttgctgctg atggtgatta tgctggcaat cccgttcctg
    18001 gcccgcaatg agttcggcgc agctattagc atggtggtgt ggggcgcagc aacctttgcg
    18061 gttgtacctc cgttacagat gcgcgtgatg catgtcgcca gtgaagcgcc aggtctgtct
    18121 tcatcagtca atattggtgc ctttaatctt ggaaatgcgc tgggagcagc tgctggtggt
    18181 gcggtaattt ccgctgggct gggatacagc tttgtgccgg tgacgggggc gattgtcgcg
    18241 ggactggcat tattgctggt gtttatgtca gccagaaaac aacctgaaac agtttgcgtt
    18301 gctaacagct aattaaacaa aaacgcagaa ggataacctt ctgcgttaca gttgctgaca
    18361 acgtgcgatt tatcttgccg aatgcgctgt taacgcctca tccgcgttgg catggtaagc
    18421 gaagcgcatc aggcaatgct gcatttgcca tcagttatta tgcagcgaga tttttcgcta
    18481 cgaattccca gttcaccagc gcccagaagt gctccagata gccaggacgt gcattgcgat
    18541 agtcgatgta ataagcgtgt tcccagacat caacggtcag cagcggagtc gcatcggtgg
    18601 tcagcggagt acccgcgtta gaggttgaaa cgatagccag tttgccatcg ctgtttttca
    18661 ccagccaggt ccagccagaa ccaaagtttt tgatcgctgc atcagtaaac tgcgctttga
    18721 aatcggcaaa gctgccaaaa gatgcggcga tagcttcagc gacttttcca gtcggttcgc
    18781 caccggcgtt cggtgccagg cagttccagt agaaagtatg gttccagacc tgagctgcgt
    18841 tgttgaatac gccaccttca gagctgcgaa taatctcttc cagtgattta ccttcaaacg
    18901 cggtaccttt aatcaggttg ttcaggttag tgacataagt ctgatgatgc ttgccgtagt
    18961 gatactcgat agtttccgca gaaatgtgcg gtgccagagc atctttagca tatggtagtg
    19021 caggtaattc gaatgacatt gctactctcc tttattatta atttgcatac gtacaatagc
    19081 cttattgtgc gtatggatag ggtagcaaat taaaaggtga gacaaaagat gaacttaccc
    19141 tgttgccgta acaacagggt aaagttttag cgaagtgttt ttggggtcat tacccgacga
    19201 gcgccaacat agtggcgctg ccagtagtct tcactgagag aagtgatttg aatttcctga
    19261 cctgtgcgcg gtgactgaat aaatttgccg ttgccgacat acacgccgac atgatcggct
    19321 gtaccgcgtc cctgagtacg gaaaaagacc aggtcgccgt ttttcagctc actacgttcg
    19381 ataggtcctg catcacgcag gtgatacatt tcattcgccg tacgcggaat acgaattttc
    19441 accaaatctt tataagcgta ataaaccagg ccgctgcaat caaaaccggt acgcggtgag
    19501 ctgccgcccc aacgatatgg cttaccaatt tgctgcatca gtttattcat tgccactttc
    19561 gtggcttttt gcaccttcgc tttgtgcgcg tcggcaaggg ttcctgacgc ggcatttttg
    19621 actttcacac aatgcgattt atagccctta cgtttggtac acttttcggt aaagctggct
    19681 gaggccgttt tattgacggc gtgtttgctg cgagttttgg cgtttttaga agcggtaatg
    19741 gaagattttt taacagaaga agcggttttg cttttggtca tacttttact ggcggctttt
    19801 ttcgctgttt tctggctggc tttctgggtc tttttggtgg ttgccgtgct tttcttttta
    19861 tctgcttttt tggtgatatg agtggtagca gaactctccc tggcttgctt tgaagcatgg
    19921 gccataggcg ttaaaggcag ggtggtaaaa agtaaagcac agagcgtgat cgaaatacgg
    19981 tttatccgcg ccactgagcg tccctctgtt aaggcaggtc tatcatgcct gcgtctgatt
    20041 tacaaaactt gatgattcta atgcatagaa gaagagaaag ttaatagctt ttattgttaa
    20101 aaaatacgtt tcgttggcga gcgcaaaaaa atgcagcagt gaaagctaaa aatggttata
    20161 aatacgccag tttgccgcgc atcagagaag aaacgaatat ttgaaatgca acaaatagag
    20221 caaagggata aaatagcaaa agcgtgatga aaatgttatt ttccgatgcc gcactgaccc
    20281 gctacaatgt cagattactg ccgtaaagaa gtttgaggaa gcaagacaat gagcaccact
    20341 atcgaaaaaa tccaacgcca gattgctgaa aacccgatcc tgctgtacat gaaaggttca
    20401 ccgaaactgc cgagctgcgg tttctctgcc caggcagtcc aggcgcttgc cgcatgtggc
    20461 gaacgttttg cctatgttga tattctgcag aatccggaca ttcgtgcgga actgccgaaa
    20521 tatgctaact ggccgacctt cccgcaactg tgggttgacg gcgagctggt cggcggttgt
    20581 gatatcgtga tcgaaatgta tcagcgtggc gaactgcagc agctgatcaa agaaactgcc
    20641 gctaaataca agtctgaaga gccggacgcg gaataatccg tcctgcaata ctgacggatg
    20701 cgaacgggca tccgtcagtc ctttatccgg tgcgccgtaa aacctcgtcc ttcagggcgg
    20761 ggatataagg cgcggttttc cacctgacca ggtgtttgct gttgttcaat gtactggcgg
    20821 atgacggata ttggcgcacc ttcgcagcta ctggcaaaat agccaggact ccacaaaacg
    20881 cctttgtagt aatacctgac tgcaatatct ggtcgatctc gtcgcagtaa cctattcgat
    20941 atgcctttga ggctgtttac cagactggat atcgccagtt tgggaggata gttaattaac
    21001 aaatggacgt gatctggttc gccatccatt tcaaccagtt cagctgcaaa atcagcacat
    21061 acatttgaaa agtaagtgcg tagtttttct gtcgtgtcat ggtcaaaaat ctggcgtcgg
    21121 tatttggtga caaagaccag gtgaacatgc atcaggaaaa cacaatgcct tccacgccgg
    21181 atatcggttt cttttttcac agaccaaagt atcatttgac ctgtgaaacg attacaggca
    21241 tttaaattcc agttaagacc cggtggtcaa caggagcgtg aaatgaggcg cttcgcaggc
    21301 gcatgtcgtt tcgttttcaa tcgtgctctg gcgcttcaga atgagaatca tgaggccggt
    21361 aataaataca tcccttacgg gaaaatggct tcctggctgg ttgagtggaa aaacgccact
    21421 gaaacgcaat ggcttaaaga ttcgccctca cagccattgc aacagtcact gaaagacctt
    21481 gagcgggctt acaaaaactt cttccagaag cgggctgctt ttccccgatt caaaaagcgg
    21541 ggacagaatg ctgcattccg ctacccgcag gacgttaagc tcgatcagga aaacagccgt
    21601 atttttctgc cgaaacttgg ctggatgcgc taccggaata gccgtcaggt cacgggagtt
    21661 gtgaaaaatg tcactgtcag ccagtcctgc ggtacgtggt acatcagtat tcagacagaa
    21721 agtgaagtat caactccggt tcacccttca gcatcaatga tcgggctgga tgctggcgtg
    21781 gctaaactcg ccacgctgtc agatggcaca gtctttgagc ctgtaaacag ttttcaaaaa
    21841 aaccagaaga cgctggcgag acttcagcga cagttaagcc ggaaggtcaa attcagcaac
    21901 aactggcaga agcagaaacg caaaatacag cgactgcatt cctgtatcgc aaatatccgc
    21961 agggactacc ttcacaaagt cacaacg
//
LOCUS       DAZSIO010000057        21930 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZSIO010000057 DAZSIO010000000
VERSION     DAZSIO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.057"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..147)
                     /locus_tag="ACHX5T_001973"
     CDS             complement(<1..147)
                     /locus_tag="ACHX5T_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR8467188.1"
                     /translation="MLARDKSNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSL"
     gene            complement(157..708)
                     /gene="ybcL"
                     /locus_tag="ACHX5T_001974"
     CDS             complement(157..708)
                     /gene="ybcL"
                     /locus_tag="ACHX5T_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR8467189.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(877..1209)
                     /gene="emrE"
                     /locus_tag="ACHX5T_001975"
     CDS             complement(877..1209)
                     /gene="emrE"
                     /locus_tag="ACHX5T_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR8467190.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(1554..1868)
                     /gene="fliE"
                     /locus_tag="ACHX5T_001976"
     CDS             complement(1554..1868)
                     /gene="fliE"
                     /locus_tag="ACHX5T_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR8467191.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2082..3741
                     /gene="fliF"
                     /locus_tag="ACHX5T_001977"
                     /pseudo
     CDS             2082..3741
                     /gene="fliF"
                     /locus_tag="ACHX5T_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
     gene            3734..4729
                     /gene="fliG"
                     /locus_tag="ACHX5T_001978"
     CDS             3734..4729
                     /gene="fliG"
                     /locus_tag="ACHX5T_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR8467192.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4722..5408
                     /gene="fliH"
                     /locus_tag="ACHX5T_001979"
     CDS             4722..5408
                     /gene="fliH"
                     /locus_tag="ACHX5T_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR8467193.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5408..6781
                     /gene="fliI"
                     /locus_tag="ACHX5T_001980"
     CDS             5408..6781
                     /gene="fliI"
                     /locus_tag="ACHX5T_001980"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR8467194.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6803..7144
                     /gene="fliJ"
                     /locus_tag="ACHX5T_001981"
                     /pseudo
     CDS             6803..7144
                     /gene="fliJ"
                     /locus_tag="ACHX5T_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
     gene            7141..8268
                     /locus_tag="ACHX5T_001982"
     CDS             7141..8268
                     /locus_tag="ACHX5T_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR8467195.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8373..8837
                     /gene="fliL"
                     /locus_tag="ACHX5T_001983"
     CDS             8373..8837
                     /gene="fliL"
                     /locus_tag="ACHX5T_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR8467196.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8842..9846
                     /gene="fliM"
                     /locus_tag="ACHX5T_001984"
     CDS             8842..9846
                     /gene="fliM"
                     /locus_tag="ACHX5T_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR8467197.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9843..10256
                     /gene="fliN"
                     /locus_tag="ACHX5T_001985"
     CDS             9843..10256
                     /gene="fliN"
                     /locus_tag="ACHX5T_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR8467198.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10259..10624
                     /gene="fliO"
                     /locus_tag="ACHX5T_001986"
     CDS             10259..10624
                     /gene="fliO"
                     /locus_tag="ACHX5T_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR8467199.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            10624..11389
                     /gene="fliP"
                     /locus_tag="ACHX5T_001987"
                     /pseudo
     CDS             10624..11389
                     /gene="fliP"
                     /locus_tag="ACHX5T_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            11399..11668
                     /gene="fliQ"
                     /locus_tag="ACHX5T_001988"
     CDS             11399..11668
                     /gene="fliQ"
                     /locus_tag="ACHX5T_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR8467200.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11677..12462
                     /gene="fliR"
                     /locus_tag="ACHX5T_001989"
     CDS             11677..12462
                     /gene="fliR"
                     /locus_tag="ACHX5T_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR8467201.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            12747..13371
                     /gene="rcsA"
                     /locus_tag="ACHX5T_001990"
                     /pseudo
     CDS             12747..13371
                     /gene="rcsA"
                     /locus_tag="ACHX5T_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
     gene            complement(13415..13603)
                     /gene="dsrB"
                     /locus_tag="ACHX5T_001991"
     CDS             complement(13415..13603)
                     /gene="dsrB"
                     /locus_tag="ACHX5T_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR8467202.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13766..13944
                     /locus_tag="ACHX5T_001992"
                     /pseudo
     CDS             13766..13944
                     /locus_tag="ACHX5T_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2525 domain-containing protein"
     gene            14242..15057
                     /locus_tag="ACHX5T_001993"
                     /pseudo
     CDS             14242..15057
                     /locus_tag="ACHX5T_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(15054..16748)
                     /gene="dgcQ"
                     /locus_tag="ACHX5T_001994"
                     /pseudo
     CDS             complement(15054..16748)
                     /gene="dgcQ"
                     /locus_tag="ACHX5T_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
     gene            complement(16919..17101)
                     /locus_tag="ACHX5T_001995"
     CDS             complement(16919..17101)
                     /locus_tag="ACHX5T_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR8467203.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(17180..18097)
                     /locus_tag="ACHX5T_001996"
     CDS             complement(17180..18097)
                     /locus_tag="ACHX5T_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR8467204.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            18270..19190
                     /gene="yedA"
                     /locus_tag="ACHX5T_001997"
     CDS             18270..19190
                     /gene="yedA"
                     /locus_tag="ACHX5T_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFR8467205.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(19179..19649)
                     /gene="vsr"
                     /locus_tag="ACHX5T_001998"
     CDS             complement(19179..19649)
                     /gene="vsr"
                     /locus_tag="ACHX5T_001998"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR8467206.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(19630..21048)
                     /gene="dcm"
                     /locus_tag="ACHX5T_001999"
     CDS             complement(19630..21048)
                     /gene="dcm"
                     /locus_tag="ACHX5T_001999"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR8467207.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(21115..21616)
                     /locus_tag="ACHX5T_002000"
                     /pseudo
     CDS             complement(21115..21616)
                     /locus_tag="ACHX5T_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            complement(21695..>21930)
                     /gene="drpB"
                     /locus_tag="ACHX5T_002001"
     CDS             complement(21695..>21930)
                     /gene="drpB"
                     /locus_tag="ACHX5T_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR8467208.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRSARCRRYE"
BASE COUNT         5370 a         5787 c         5574 g         5199 t
ORIGIN      
        1 tagactatgc gtctcccaat tgataacttt ttttccctga cggatacgac ataaagctgg
       61 cataagaggc ttaaccctgt gaatctcatg atgtttatgc atccgtattt cttcaatctt
      121 taggttagac ttatctcttg ccagcatact cttaccctat tttatctcat aaactggtgt
      181 tatctcagcg gttgcaattt tattggcatt aagcatataa ccaactaacg ctccgctgga
      241 attagaatcc acaggaatct tatcagtttt tagagcccat actttaaact ggtaatgatg
      301 gggtttatct cctttgggag gacatgctcc accaaatcct gcataaccaa aatcatttcg
      361 gccttgaaca gcaccagtcg gaagttttgt accatcacgt cttcctgcat cagtgggcaa
      421 atatgttaca gttgctggaa tattagcaac agtccagtgc caccatccac tgcctgtagg
      481 agcatctgga tcatacacag ttacggcaaa gcttttggtg ccttcaggag caccagacca
      541 ggttaatgag ggcgatgtat taccaccttc acacccaaat ccagaaaata catgagacgt
      601 tgtaagttgc tctcctgttt ttatttcatt actagtgacc tgaaatgctg cggcctgcgc
      661 agaaaatgtt atgaatgcca atacacttga aacgataagt tttttcataa aaacctcatt
      721 gttgtgacct atcgttattt tatttgatgt gccttgatct cattatgcat aaaggcgcaa
      781 tgatcgcgca aatgcaatca taattataaa ggtgccccga acattagcca taatatgcac
      841 aaatcagatt cctgttatct caaggagaat tattttttaa tgcggtgcac ttcgtgacaa
      901 caagttaatg actaacacac cggcacaaat caacatcatg cctatgatag ctggcaggtc
      961 cagtcgttga ccaaaaaatc cccaggaaag caaactaatc agaacgatac cgactcctga
     1021 ccagatagca taagcaatcc ctgtaggtat ataagccagc gtctgagcta ataaccagaa
     1081 tgatgcacaa tagcaaataa ttgtgccaac agagggccat aaccgtgtaa acccctctga
     1141 aaacttcatt aaggttgtac caatgacctc tgcaagtatt gcgccaccaa gataaatata
     1201 agggttcata gcatattctt tcctgttcat actggagaga attgtactac agtttgaaca
     1261 caactcaccg ttttcatcct ggtgtatgtg tatccaaggg gctctggcgt ggcaagagat
     1321 aaaggcagga acaataatga tcagaaggaa ccccaggcta acgaatgagt tcactcttcc
     1381 tgggagcaaa gcgaagacaa aacatgttat cgcgtttacg gatcgtggat ggcagaaaac
     1441 aaccaggatc aggtatccat tttaaaccag aaattgagtg agtttgcccc atccatgccc
     1501 cacgctgtgg gatcggatat aattaaacaa gcatagtcaa caggttacac aacctacacc
     1561 tgcatggaca tcacttcctg atacgccgcc accagcttat tacgcacctg aatccccatt
     1621 tgcatagaaa ctgaggcttt ttgcatatcg gtcatcacat cgtttagcgc cacgccgggt
     1681 tcaccgaggg tgaatttttc tgcctgcgtg cgggcgactg tttgtgtatc gcttatgcga
     1741 tcgagcgcgg cgtgcagctg cccggcaaaa ctaatggtcg gttgcggcag tgattcctgc
     1801 gcacgcgcac tcatcgccgt agcctgtaac tggctgataa ccccttcaat cccctgtatc
     1861 gctgacattt tcgtctcccg gataatttct ggtaacaaag cctaccagta agtcaataag
     1921 acaaaggcgc taaatagcaa caaaaaaact ggtttattgg cggatagaaa aaacgaaagc
     1981 acaaataatg ggagcgtcaa tttttcgagt gtgctgaccc gagagtgagt cttgttccac
     2041 tttgccaata acgccgtcca taatcagcca cgaagtgcgc gatgaatgcg actgcagccc
     2101 agacaaaatc tcttgagtgg cttaatcgcc tgcgtgcgaa tccgaaaatt ccattgattg
     2161 ttaccggttc cgcggcagtg gcggtcatgg tcgcactgat cctgtgggcg aaagcccccg
     2221 actaccgcac attattcagc aatctttccg atcaggatgg tggcgcaatt gtcagccaac
     2281 tgacgcaaat gaatattcct taccgcttca gcgaagccag cggcgctatt gaggttccgg
     2341 cagataaagt tcacgaactg cgcctggcgc aacaaggttt gcccaaaggc ggcgcggtcg
     2401 gtttcgaact gctcgatcag gaaaagtttg gtatcagcca gttcagcgaa caggtgaatt
     2461 atcagcgggc gctggaaggc gagctttctc gtaccatcga aactatcggc ccggtaaaag
     2521 gggcgcgcgt acatctggca atgccgaaac cgtctttatt cgtccgtgaa caaaaatccc
     2581 cttctgcatc ggtgacggta aatctgttac ccggccgcgc actcgatgaa gggcaaatta
     2641 gcgctattgt acatctggtt tccagcgcgg ttgctggtct gccgcctgga aacgtcacgc
     2701 tggtggatca gggcgggcat ctgttaaccc agtccaatac cagcgggcgc gatcttaatg
     2761 acgctcagtt gaaatatgcc agcgatgtcg aaggccgtat tcagcagcgt attgaagcga
     2821 tcctgtcacc tattgttggt aacggtaata ttcacgccta ggtcacggcg caactggact
     2881 tcgccagtaa agaacaaacg gaagaacagt atcgccctaa cggtgatgaa tctcatgcgg
     2941 cgcttcgttc acgccagctt aatgagagcg agcaaagcgg ttccggttat ccgggcggcg
     3001 taccgggggc gttgtcgaat caaccggcac ctgcgaataa cgcgccaatc agcacgcctc
     3061 cgacaaatca aaataaccgc cagcagcagg cgagcaccac cagcaatagt gggccgcgta
     3121 gcacacagcg gaatgaaacc agtaactacg aagtcgatcg caccattcgt cataccaaaa
     3181 tgaacgtggg cgatgtgcaa cgtctgtcag tcgcggtagt ggtgaattac aaaaccttgc
     3241 cagacggtaa accattgcct ctcagcaacg aacagatgaa gcaaattgaa gctctgaccc
     3301 gcgaggcgat gggcttttct gaaaaacgcg gtgattcgct caatgttgtt aactcgccgt
     3361 tcaatagcag tgacgaaagc ggcggagcac tgccgttttg gcaacagcaa gtgtttatcg
     3421 atcagttgct ggctgccggt cgctggttgc tggttgctgg tactgctggt ggcgtggttg
     3481 ctgtggcgga aagcggtacg cccgcagcta acacgtcgcg ccgaggcggt gaagactgtg
     3541 cagcaacagg cgcaggcccg cgaggaagtg gaagatgcgg tggaagtccg tctgagcaaa
     3601 gacgaacaac tccaacaacg gcgcgctaac caacgactgg gggcagaagt catgagccag
     3661 cgtatccgtg aaatgtctga taacgatccg cgcgtggtgg cgctggtcat tcgccagtgg
     3721 ataaataacg atcatgagta acctgacagg caccgataaa agcgtcatcc tgctgatgac
     3781 cattggcgaa gaccgggcgg cagaggtgtt caagcacctc tcccagcgcg aagtgcaaac
     3841 cctgagcgct gcaatggcga acgtcacgca gatctccaac aagcagctaa ccgatgtgct
     3901 ggcggagttt gagcaagaag ctgaacagtt tgctgcactg aatatcaacg ccaacgatta
     3961 tctgcgttcg gtattggtca aagctctggg tgaagaacgt gccgccagcc tgctggaaga
     4021 tattctcgaa acccgcgata ccgccagcgg tattgaaacg ctcaacttta tggagccgca
     4081 gagcgccgcc gatctgattc gcgatgagca tccgcaaatt atcgccacca ttctggtgca
     4141 tctgaggcgc gcccaggccg ccgatattct ggcgttgttc gatgaacgtc tgcgccacga
     4201 cgtgatgttg cgtatcgcca cctttggcgg cgtgcagcca gccgcgctgg ccgaactgac
     4261 cgaagtactg aatggcttgc tcgacggtca gaatctcaag cgcagcaaaa tgggcggcgt
     4321 gagaacggca gccgaaatta tcaacctgat gaaaacgcag caggaagaag ccgttattac
     4381 cgccgtgcgt gaattcgacg gcgagctggc gcagaaaatc atcgacgaga tgttcctgtt
     4441 cgagaatctg gtggatgtcg atgatcgcag cattcagcgt ctgttgcagg aagtggattc
     4501 cgaatcgctg ctgatcgcgc tgaaaggggc cgagcagcca ctgcgcgaga aattcctgcg
     4561 caatatgtcg cagcgtgccg ccgatattct gcgcgacgat ctcgccaagc gtggtccggt
     4621 gcgtctgtcg caggtcgaaa acgaacagaa agcgattctg ctgattgtgc gccgtcttgc
     4681 cgaaactggc gagatggtga ttggcagcgg cgaggatacc tatgtctgat aatctgccgt
     4741 ggaaaacctg gacgccggac gatctcgcgc caccaccggc agagtttgtg ccaatggtcg
     4801 agtcggaaga aaccattatt gaagaggccg aacccagcct tgagcagcaa ctggcgcaac
     4861 tgcaaatgca ggcccacgag cagggttatc aggcggggat tgccgaaggt cgccagcaag
     4921 gtcacgaaca gggctatcag gaaggactgg cccaggggct ggaacaaggt ctggcagagg
     4981 cgaaggctca acaagcgcca attcatgccc ggatgcagca actggtcagc gaatttcaaa
     5041 ctacccttga tgcgcttgat agtgtgatag cttcgcgcct gatgcagatg gcgctggagg
     5101 cagcacgtca ggtgatcggt cagacaccaa cggtggataa ctcggcactg atcaaacaga
     5161 tccaacagtt gttgcagcaa gaaccgttat tcagcgggaa accacagctg cgcgtacacc
     5221 cggatgatct gcaacgtgtg gatgatatgc tcggcgcaac tttaagtttg catggctggc
     5281 gcttgcgggg cgatcccacc ctccaccctg gcggatgtaa agtctccgcc gatgaaggcg
     5341 atctcgacgc cagtgtcgcc actcgctggc aagaactctg ccgtctggca gcaccaggag
     5401 tggtgtaatg accacgcgcc tgactcgctg gctaaccacg ctggataact ttgaagccaa
     5461 aatggcgcag ttgcctgcgg tacgtcgcta cgggcgatta acccgcgcta ccgggctggt
     5521 gctggaagcc accggattac aattgccgct cggcgcaacc tgtgtcattg agcgccagaa
     5581 cggcagcgaa acgcacgaag tagaaagcga agtcgttggc tttaacggtc aacggctgtt
     5641 tttaatgccg ctggaggaag tggaaggtgt cctgcccggc gcgcgtgtat atgccaaaaa
     5701 catttcggca gaagggctgc aaagcggcaa gcagttgccg ctcggtccgg cgttattagg
     5761 tcgtgttctg gacggtagcg gtaaaccgct cgatggcctg cccttccccg atacgacgga
     5821 aaccggtgcg ctgattaccc cgccatttaa cccgttgcaa cgtacaccga ttgaacatgt
     5881 gctggacacc ggcgtgcgcc caatcaatgc cctgcttacc gtcggacgcg ggcagcgtat
     5941 ggggctgttt gccgggtcgg gcgttggtaa aagtgtgctg ctggggatga tggcacgcta
     6001 cacccgcgcc gatgtcattg tcgtgggttt gattggtgaa cgtgggcgcg aagtaaaaga
     6061 ttttattgag aatatcctcg gtgccgaagg acgtgcacgc tcagtggtga ttgccgctcc
     6121 ggcggatgtt tctccgctcc tgcgaatgca aggtgccgcc tatgccacgc gcattgccga
     6181 agattttcgc gatcgtggtc agcatgtgtt gctgattatg gactccctca cccgctacgc
     6241 gatggcccag cgtgaaattg cgctggcgat tggcgaacca cctgccacta aaggttatcc
     6301 accgtcggtg tttgccaaat taccggcact ggtcgagcgt gccggtaatg gcattagcgg
     6361 cggcggctcg attaccgcgt tttataccgt gctcaccgaa ggcgatgacc agcaggaccc
     6421 cattgctgac tccgcgcggg caatcctcga cggtcacatt gtgctgtctc gccgactggc
     6481 ggaagccggg cactatccgg ctatcgatat tgaagcgtcg atcagtcgcg caatgacggc
     6541 gttgatcagt gagcaacatt acgcgcgagt gcgcaccttc aaacagctgt tgtcgagttt
     6601 tcagcgtaac cgcgatctgg ttagcgtcgg cgcgtatgcc aaaggcagcg atccgatgct
     6661 cgataaagcc atcgccctgt ggccgcagct ggagggctat ttgcaacaag gcatttttga
     6721 acgcgcggac tgggaagcgt ctctccaggg gctggagcgt attttcccga cagtgtcata
     6781 acccaggaga taacggcaga tggcggaaga acagctcaaa atgttgatcg attatcagaa
     6841 tgaatatcgc aataacctca atagcgatat gagtgccggg atgaccagca accgctggat
     6901 caactatcag cagtttatcc agacgctgga aaaagccatt actcagcatc gccagcaact
     6961 taatcagtgg acgcagaaag ttgacattgc cctgaacagc tggcgagaaa agaaacaacg
     7021 tttgcaggcc tggcagacac tgcaggaacg gcaatccacg gcggcactgc ttgcagaaaa
     7081 ccgcctcgat cagaaaaaga tggatgagtt cgcccagcgc gccgccatga ggaaacctga
     7141 atgattcgct tagcgccctt gattaccgcc gacgttgaca ccaccacatt gcctggcggc
     7201 aaagccagcg atgctgcaca agattttctc acgttgttga gcgaagcatt agcaggcgag
     7261 acaacaactg acaaagcggc cccccagttg ctggtagcaa cagataagcc cacgacaaaa
     7321 ggcgagccgc tggtcagcga tattcttgcc gatgcgcaac aagcggattt actgatccct
     7381 gtggatgaaa cacttcctgt catcaacgac gaacaatcca catcaacacc gttaaccacc
     7441 gctcagacga tgacgttggc tgcggtggct gacaaaaata cgacaaaaga cgaaaaagcg
     7501 gatgatctta atgaagacgt caccgcaagt ctgagcgccc tttttgcgat gttgccgggt
     7561 tttgacaata cgcccaaagt gactgatgca ccgtcaaccg tgttaccggc agagaaacca
     7621 acgctcttca caaaactgac ttctgcgcaa ctcactacag cacagcctga tgacgccccc
     7681 ggcacaccag ctcagccatt aacaccgctg gtagcagaag cccagagtaa agcggaagtc
     7741 atcagcacgc cttcgccggt gaccgctgac gccagcccgc taatcactcc acaccagaca
     7801 cagccactgc ccaccgtcgc cgcgcctgtt ttgagtgcac cgctgggttc tcacgaatgg
     7861 caacaatcat tgagccagca tatttcgctg ttcacccgcc aggggcaaca aagtgcagag
     7921 ttgcgtctgc acccacagga tttaggtgaa gtgcaaattt ccctcaaagt ggatgataac
     7981 caggcgcaga tccagatgat ttcaccgcat cagcacgtac gcgccgccct ggaagcagcg
     8041 ctgccggtac tgcgtacgca gctggccgaa agtggcattc agttagggca aagcaacatt
     8101 agtggcgaaa gctttagtgg tcagcagcag gccgcttccc agcaacagca aagccaacgc
     8161 acagcaaacc atgaacctct ggcgggggaa gacgacgata cgcttccggt tcccgtctct
     8221 ttacaagggc gtgtaacagg taacagcggc gttgatattt tcgcctaacg tcagaggtag
     8281 caccgtaatc cgcgtctttt ccccgctttg ttgcgctcaa gacgcaggat aattagccga
     8341 taagcagtag cgacacagga agaccgcaac acatgactga ttacgcgata agcaagaaaa
     8401 gcaagcgatc gctttggatc ccgattctgg tattcattac cctcgcggcc tgtgccagcg
     8461 caggttacag ctactggcat tcgcatcagg ttgccgctga cgacaaagcg cagcaacgcg
     8521 tcgtgccctc cccggtcttc tatgcgctgg atacctttac ggtcaatttg ggcgatgcgg
     8581 atcgcgtgct ttatatcggc ataaccctgc gactgaaaga tgaagcaacc cgctcgcggc
     8641 tgagtgagta tttgccggaa gtccgtagtc gcttgctgtt actgttttca cgtcaggatg
     8701 ctgccgtact ggcgacagaa gaaggcaaga aaaacctgat tgccgagatt aaaaccacac
     8761 tttccacccc gcttgttgcc gggcaaccga aacaggatgt caccgacgtg ctgtataccg
     8821 cttttattct gcgataacga catgggcgat agtattcttt ctcaagctga aattgatgcg
     8881 ctgttgaatg gtgacagcga agtcaaagac gaaccgacag ccagtattag cggcgaaagt
     8941 gacattcgtc cgtacgatcc gaatacccaa cgacgggttg tgcgcgaacg tttgcaggcg
     9001 ctggaaatca ttaatgagcg ctttgcccgc cattttcgta tggggctgtt caacctgctg
     9061 cgccgtagcc cggacattac cgtcggggcc atccgcattc agccgtacca tgaatttgcc
     9121 cgcaacctgc cggtgccgac caacctgaac ctaatccacc tgaaaccgct acgcggcaca
     9181 gggctggtag tgttctcgcc gagtctggtg tttatcgccg tggataacct gtttggcggc
     9241 gatggacgct tcccgaccaa agtggaaggt cgcgagttta cccatactga acagcgcgtc
     9301 atcaaccgta tgttgaagct ggcgctggaa ggctatagcg acgcctggaa agcgattaat
     9361 ccgctggaag ttgagtacgt gcgttcggaa atgcaggtgg aatttaccaa tatcaccacc
     9421 tcgccgaacg acattgtggt taacacgccg ttccatgtgg agattggcaa cctgaccggc
     9481 gaatttaata tctgcctgcc attcagcatg atcgagccgc tacgggaatt gttggttaac
     9541 ccgccgctgg aaaactcgcg taatgaagat cagaactggc gcgataacct ggtgcgccag
     9601 gtgcagcatt cacagctgga actggtggcc aactttgccg atatctcgct acgcctgtcg
     9661 cagattttaa aactgaaacc cggcgacgtc ctgccgatag aaaaacccga tcgcatcatc
     9721 gcccatgttg acggcgtccc ggttctgacc agtcagtacg gtactctcaa cggtcagtat
     9781 gcgttacgga tagaacattt gattaacccg attttaaatt ctctgaacga ggaacagccc
     9841 aaatgagtga catgaataat ccggccgatg acaacaacgg cgcaatggac gatctgtggg
     9901 ctgaagcgtt gagcgagcaa aaatcaacca gcgaaaaaag cgctgccgac gcggtgttcc
     9961 agcaatttgg cggtggtgat gtcagcggaa cgttgcagga tatcgacctg attatggata
    10021 ttccggtcaa gctgaccgtc gagctgggcc gtacgcggat gaccatcaaa gagctgttgc
    10081 gtctgacgca agggtcagtc gtggcgctgg acggtctggc gggcgaaccg ctggatattc
    10141 tgatcaacgg ttatttaatc gcccagggcg aagtggtggt tgttgccgat aaatatggcg
    10201 tgcggatcac cgatatcatt accccgtccg agcggatgcg ccgcctgagc cgttagtgat
    10261 gaataaccat gctactgtgc aatcttccgc gccggtttct gctgagccac tgctgcaggt
    10321 gagcggcgca ctcatcgcca ttattgccct gatcctcgct gctgcctggc tggtaaaacg
    10381 gctgggattt gcccctaaac gcactggcgt taacggtctg aaaattagcg ccagtgcttc
    10441 gctgggcgcg cgtgaacggg tcgtggtggt cgatgtggaa gatgcacggc tggtgctcgg
    10501 cgttaccgca gggcaaatca atctgctgca taaacttccc ccttcagcac caacggaaga
    10561 gataccgcag accgattttc agtcggtcat gaaaaatttg cttaagcgtg gcgggagatc
    10621 ctgatgcgtc gtttgttttc tgtcgcaccg gtccttctct ggctgattac gcccctcgcc
    10681 ttcgcgcaac tgccgggtat caccagccag ccgctacctg gcgggtatca ccagccagcc
    10741 gctacctggc ggtggacaaa gctggtcgct cccggtgcag acgctggtgt tcatcacctc
    10801 gttgacgttc attccagcga ttttactgat gatgaccagt ttcacccgca tcattattgt
    10861 ttttggttta ttgcgtaacg cgctgggaac gccctccgcg ccacctaacc aggtattgct
    10921 ggggctggca ctgtttttga ccttttttat tatgtcgcca gtgatcgaca aaatttatgt
    10981 cgatgcgtac cagccattca gcgaagagaa aatctcaatg caggaggcgc tggaaaaagg
    11041 ggcgcagccg ctgcgtgagt ttatgctgcg tcagacccgc gaggcagatt tagggttgtt
    11101 tgccagactg gcgaataccg gcccgttgca ggggcctgaa gctgtgccga tgcgcatttt
    11161 gctcccggcc tacgtgacca gcgagttgaa aaccgcattt cagataggct tcacgatttt
    11221 catccctttt ttgattatcg acctggtgat tgccagcgtg ttgatggcat tggggatgat
    11281 gatggttccc cccgccacca ttgctctgcc ctttaaactg atgctgtttg tactggtgga
    11341 tggctggcaa ttgctggtcg gttcgctggc gcagagcttt tacagctaga gaggcaaaat
    11401 gacacctgaa tcggtcatga tgatgggaac tgaagcgatg aaagtcgcgc tggcactggc
    11461 agccccgctg ttgctggtag cgctggtcac gggccttatc atcagtattt tgcaggcagc
    11521 cacgcagatt aacgaaatga cgctgtcgtt tattccgaaa atcatcgccg tatttatcgc
    11581 catcattatt gccggaccgt ggatgctcaa tctgttgctg gattacgtcc gcaccttgtt
    11641 cactaacctg ccgtatatca tcgggtagcc cgtacaatga tgcaggagac aagcgatcaa
    11701 tggctttcct ggttaagcct gtacttctgg ccgttactgc gcgtgctggc gctcatttct
    11761 accgcgccaa ttctgagcga acgcagcgtg ccgaaacggg taaaactggg tctggcaatg
    11821 atgatcacgt tcgccattgc cccatcatta cctgccaacg atgttcctgt tttttcgttc
    11881 tttgctctgt ggctggccgt acagcagatc ctgatcggca ttgcgcttgg ttttaccatg
    11941 caatttgcct ttgccgctgt gcgaaccgct ggcgaaatta tcggtctgca aatggggctg
    12001 tcatttgcga catttgtcga tcctggcagc catctcaata tgcccgtttt agcgcgcatc
    12061 atggatatgc tggcattact gctgttcctg acatttaacg gtcatttatg gttgatttca
    12121 ctgctggtcg atacctttca caccctgccg attggtagcg aaccgttgaa cagcaatgcg
    12181 tttctggcac ccaccaaagc agggagtttg attttcctta acgggctgat gctggcgtta
    12241 ccgctcatta ctctgttgct gaccctgaat ctggcattag gtttactgaa tcgtatggcc
    12301 ccgcaattat ccatttttgt tattggattt ccattaactc tgactgtcgg catctcttta
    12361 atggcggcat taatgccgtt aattgcgcct ttttgcgaac atttattcag tgaaattttt
    12421 aatttgctgg ctgatattat tagtgaatta ccattaatat aattccgtaa cgtttatcat
    12481 gctaccctaa ggattatccg aaaaataata tctgcgaaca tcttccagga tactcctgta
    12541 gcgaaatatt tgttttaagc tcactcacat atcgcaacat ttactttaag acaattccag
    12601 gcaaattata caacacttta cgggatagta agtccgcctg aaaaattgcg agagtggcgc
    12661 attaggtgac ccatgttgtt ccgtttagtc atgatgaaat attcaggtaa ggggaattat
    12721 cgttacgcat tgagtgaggg tatgccatgt caacgattat tatggattta tgtagttaca
    12781 cccgactagg tttaaccggg tatctgttga gtagaggggt taaaaaaaga gaaatcaacg
    12841 acattgaaac cgttgatgac cttgccatag cttgtgattc acagcgtcct tcagtggtgt
    12901 ttattaatga ggactgtttc atccacgatg cttctaacag tcagcgcatc aagcacatca
    12961 ttaatcaaca tcccaatacg ttatttatcg tttttatggc aattgccaat gttcattttg
    13021 atgaatatct attggtcaga aaaaaattta ttgatcagtt ctaaatcgat taaaccggaa
    13081 tctctcgacg atatccttgg cgatattctg aaaaaagaga caacgataac ctcgttttta
    13141 aatatgccga cgttatcatt gagccgaacc gaatcgagta tgttgcgaat gtggatggca
    13201 ggtcagggaa ccattcaaat ctccgaccaa atgaatatca aagccaagac cgtttcatcg
    13261 cataaaggta atattaaacg taagatcaaa acgcataata aacaggttat ctaccatgtc
    13321 gtccgactga cggataatgt gactaatggt atttttgtca acatgcgcta acacattctg
    13381 actggtggtt tcccaccagt caggctgaat aagattactc tgctttctca acaaagatac
    13441 cgtcctgatg ccctgcttca ttaaagaacc agatgccgag cgggtagtct tccagcgcaa
    13501 ccaggtacat tgtgccttca ctaaactcct caactgccag taccactcca ggacgacgcg
    13561 gaccgccatc cgttttgact gttacccgat cattcacctt catcgttttc ctcctgtggc
    13621 tttgtgccag tgtagaacaa tttcgttttt ctggcagcgc caagcgcgcg cgagtgctga
    13681 ttttctcgac ggtctatact taagagatgc caggcggact taacgactgg cggcaacaac
    13741 agagtaacgg ttgcgaggaa agatgatgaa aaccgcaaaa gtgtacagcg ataccgcaaa
    13801 acgtgaggtc agcgtcgatg tcgccgccag aacgattctg aacacgttag cgtcgatgga
    13861 cgtgaatatc atacatggcg tgaattggcg gatgccttcg aactggatat tcatgacttc
    13921 agcgtctctg aagtgaatcg ttgaatgcac aataaaaaaa tcccgaccct gagggtgtcg
    13981 ggatgaaact tgcttaagca agaagcactt aaaaaattcg ttacaccagg aaatctgatg
    14041 tgttcatcac cttatctgca atttttttcg ctgacaagaa aatattcgcg ctatgaatga
    14101 ttaacgctac ttatgaatag aaatgtgacg cgcgtcaccc cttggtggct ctggtgtcaa
    14161 cgggatcgct cccgaaaaaa tcatctttga ttatttgtgc tgttttgaga atcatcctgg
    14221 taaggggagt actgcgcgac catgttttca attcaacaac cactactggt ttttagcgat
    14281 cttgatggca ccctgctgga cagtcatagt tatgactggc aaccggcagc cccctggctc
    14341 agccgtttac acgaagccaa tattcccgtc attctctgta gcagtaaaac tttagcggaa
    14401 atgctgtact tgcaaaaaat gttggggcta caaggcttac cgctgattgc agagaatggc
    14461 gcagtgatcc agcttgctga acaatggcag gatatagacg gttttccacg catcatctca
    14521 ggtattagcc atggcgaaat ctgccaggtt ttaaatacgc tacgcgagaa agaacatttt
    14581 aaattcacga cttttgatga tgtcgacgat gcaaccattg ccgaatggac gggattaagc
    14641 cgtagctagg cggcgctgac gcagctgcat gaggcgtcgg taacgctaat ctggcgcgac
    14701 agtgacgagc gtatggcaca atttatcgct cgtctgaacg aactgggctt gcagtttatg
    14761 cagggtgcgc gcttctggca cgtactggat gcctctgccg gaaaagatca ggctgccaac
    14821 tggattatcg cgacctatca acaattgtca ggcagacgcc caaccacact tggcctgggc
    14881 gatgggccaa acgatgcgcc tttactggag gtaatggatt acgcggtgat tgtgaaaggg
    14941 ctaaaccgtg aaggggtaca tctgcatgat gaggatccgg cccgcgtctg gcgaacgcag
    15001 cgtgaaggac cggaaggctg gcgtgaaggg ctggaccatt ttttctccgc ccgttaagcg
    15061 ttatcgctcg cgaatacccg attgcgcccg gcctgttttg cgaggtaaag ccgacggtcg
    15121 gccaatgact ggagttgttc aaaatcataa tcaccggttt cctcgctgct acttaccccc
    15181 agcgaggcac tgatgcgtat cgttgtactc ttggcgatca gcatctcttt ttcatttaac
    15241 ttcaggcgaa tacgttctgc gacctccgca gcctgcgtca gattcgcgcc tggcagaatc
    15301 acacaaaact cctcaccacc gacccgcccg gcaacgtcct gcgcacgcaa ggaactgcta
    15361 attaatccgg cagcatgaga aagaacacgg tcgcccgcct gatgaccaaa gcggtcatta
    15421 atcgctttaa aatggtcaag atcgacctga atgacagaaa aaggatgttg gtgcgtctga
    15481 cacaatttag cgagcggacg ggctttttcg aacagtgcgc cacggttata taaacgcgtt
    15541 aaggtgtcgt gccacgcctg ccactgcaac gagctttgca gaacatacat gttgctgacc
    15601 atccggcgaa tcacatacca ggagaggagt aacatggtgg taaagagcgc ccacagcagg
    15661 gttaatgcaa tgctgatact gccgaaatcg ccgcgcacgc cttcgcttag cgtatggacg
    15721 cgcgccagca caccgtcgaa atggtccaga cgttcccagc taacatagcg actgtccata
    15781 cgaatgccgc cccgcgtgtc atgttccatt gcctgcgcca gcaaggccag ttcacgagga
    15841 tcaaaaatat tccctgttgg atggtcagga ttggaagagg tcaaaaatct cagcttactg
    15901 tcatagagct gatactcacc atcgaggttt ttatcgatgg cgtttcttaa aaattgctgc
    15961 atagtacgca cgggaatact catccccagc acgccatacc agtagttatt actgtctacc
    16021 ggaacactga cggtaacctg cggttcagta ttgctggcgt gttccggttg cgaagtgaac
    16081 cagcgtaccg cgcggtgacg attttctcgt tgcgaatggc cgataaacca gggttgggtg
    16141 acatagccgt aataacgcgt tggtacattg cgcgtaaaca aggtcggctg cgtcgaaacg
    16201 taaaatccag cacgcgagac atacatcgcc tgttcaacca tcgacgagga gttgtgcgcc
    16261 agtcgcagca agtaaccaac ttccagtgca gcggtaattt cattgtcgag gctttcattt
    16321 tcgcgagaca ggaaattccc ctcgctgact aatgcatccg atacgccatt gactggcagg
    16381 gtgcgccgtc ggttgagttc gatttgccag gcgtgctcgt cgcgatgctg ttcgaactcg
    16441 gttacggcat tacgcagaga ggtgaaatcc agtggcgcca cgagagcttc gcgcatgcca
    16501 ttacgcaaaa agatcagttt gtcgacgtta tactgcaaat gcttatcgag cgcgttggct
    16561 acgttttcca gatgattacg ctagctggag atataggcat cttccagcac caccacttca
    16621 cgccaggtga gcaaggtgga aaaaagcaat accacgataa agcagagatt aacgacatga
    16681 ccgggcccca ggcggcgtgc gagttttttc aaccagctct ggttttccat ttttgtctcg
    16741 tgctgcaccc tgacgactcc caacctgctt ttttatgatt ctggcataac ccggcaatag
    16801 cgtaccgcaa aacagcgata attttataat ggtggcatga taagacgcga ctatcattca
    16861 ttgcaggcat tgggacacca ggccggatgt gatccggcct ggtgttaatg ataccctttc
    16921 aaacttcttc cgcagaacga ctcccctccc ccggaacaag atcggtttca tgaaaagctt
    16981 cccgcttgac cccgtaaccg tcataccaac gacactcaac cataccgctg gagtatccag
    17041 tgacaatcat ccgcgggccg ccctctttaa ccgtaacttc ctcactaacc ataaagctca
    17101 tactcgcctc cttttttcga gtgaaacttg ttcaccttag ttgaagatgg cgaattttgc
    17161 ataatagcca gtgcgagtat taatgtgcct gaccgcgcat tttcgctact gctttcactc
    17221 ccagcaccac gatgccgccg atgataaatc caagaatcag atttaaaaca gtcggtaata
    17281 tcatcgccac cactgcactt tgctgcccgg cgatatgttc aatagcgtga tgcagcggcg
    17341 caataccatg taccacaatc ccgccgccga cgaggaacat cgccagcgtg ccgacaatcg
    17401 ataatgcttt catcagccaa ggcgcgataa tcaataaccc tttacctaat gcctgcatca
    17461 gcgcgctaga tttttccgcc agccaatacc ctaggtcatc aatcttaacg ataaccccta
    17521 ccagaccgta gacgcccacg gtcactacca gcgcgatgcc tgaaagcacc agcacctgat
    17581 taagcaacgg cgcctctgcc acaatcccca gcgtgatggc gacgatttcc gcagacaaaa
    17641 taaaatcggt acgaatcgcc cctttaattt tgtccttttc aaacttcagc ggatcctgcg
    17701 ccgccagctt ctccagacgc tgctggctct gcgctggatc ttctttatgt ttacgcgcct
    17761 ccagcatatg cagcactttc tctacccctt caaagcagag aaacgcgcca ccaatcatca
    17821 acagtggcgt aatcgcccac gggataaacg cactgatgat cagcgccagc ggcaccagaa
    17881 tcactttatt gatcagcgat cctttcgcca cgccccagac cacgggaagt tcccggttgg
    17941 cccgcacgcc tgaaacttgt tgcgcattga gcgataaatc atcccctaat acaccggcgg
    18001 ttttcttcgc cgccagtttg cccatcacag agatatcgtc cagcagtgtg gcgatatcat
    18061 cgagcaacgt cagtaaacta ctcccggcca aaagcattcc ctctcatttt tattgttgaa
    18121 gggtgtaagt atgaagcaaa aacggcagct ctgatacttg cttcacaggt caacatttat
    18181 ttaacaatca agtgcaatta tatctctcgc cagcagaatg attttcacgt ttactatgaa
    18241 ccaccttttt atttccgccg tgagggatta tgcgtttccg ccagttgtta ccgctttttg
    18301 gcgcgctgtt tgcgttgtat atcatttggg gctcaaccta ttttgtcatt cggattggcg
    18361 tgggaagctg gcctccgtta atgatggcgg gcgttcgatt ccaggcagcc ggtattttat
    18421 tgctggcatt tttgctactg cgcggacaca aacttccccc gctacgtccg ctgctcaatg
    18481 ccgcgctgat tggcctgtta ttgctggctg tcggtaatgg catggtgacg gttgccgaac
    18541 atcaaaatgt tccttccggc atcgccgccg tagtgattgc aaccgtgccc ctctttaccc
    18601 tgtgcttcag tcgcctgttt ggcattaaaa cgcgcaaact ggaatgggtg ggtattgcca
    18661 ttgggcttgc cggaatcatc atgctcaata gcggtggaaa tttaagcggc aatccgtggg
    18721 gcgcgattct gattttaatc ggctcgatta gctgggcgtt tggctcagtt tatggctcgc
    18781 gcattacctt acctgtaggg atgatggcgg gtgcgattga gatgctggcg gcaggcgtgg
    18841 tgttaatgat cgcatcgatg attgcgggtg aaaaactgac ggcgctccct tccctgtcag
    18901 gcttccttgc ggtcggctat ctggcgctgt ttggttcgat tatcgccatt aacgcttata
    18961 tgtatttaat ccgtaatgtc agtccggctc tcgccaccag ctacgcctac gttaacccgg
    19021 tggtcgcggt cttgctgggt acgggactgg gtggcgaaac gctgtcgaag attgaatggc
    19081 tggcgctcgg cgtaattgtt ttcgcggtgg tactggtcac gttgggaaaa tatctcttcc
    19141 cggccaaacc cgttgtcgcg ccagttattc aggatgcatc aagcgagtaa atgaatcccc
    19201 tgcgtgtcga tctgcgcgct ggcaccttcg ccgcagatcc actcttccag acgctcggta
    19261 agcgccgcat ccgtcagctt ctcgcgccta cgtaacgcac actcccagac aatcagtacg
    19321 cgccagccga gctcctgcaa gcgactgata tcgcggcgat cgcgttcaac atttttacct
    19381 atcttctcca gccagaactc agttcgagtc gcaggcactt taaacagata gcagtgatga
    19441 tgatgccaga agcagccgtg ggtaaatatc acgcagcgat attcatcaac gacaaaatcc
    19501 gggcgtccgg gcagactggc gtcctgaacg cgaaacgcaa ggccctgccc ggttaacaaa
    19561 ctggcgaggc gcttctcaat cgccgtatca cgcgtggcaa tcgcgcgcat atttttgctg
    19621 cgggtggcct tatcgtgaac gtcgaccatg ttgtgcctct tgctgacgca acgccaccgc
    19681 ctgtttgatt tttggctcaa gcagttttgc caccgcggca aagaccggca cgaccaccga
    19741 gttgccgaac tggcgatagg cctgagtgtc cgaaaccgga atgcggaatt tcgcttctcc
    19801 cggcgcttca aaacccatta agcgcgcgca ttcacgaggc gttaaccgac gtggacgatg
    19861 ttgctgattc agcggatcgt caaagtcttt ctcacccgtg gccatatccc aaccgcgatc
    19921 gattaaaatt tccgcgccat ctttgtaata acgagcagac agcgtacggg tgacgctttg
    19981 cggattgttc ggataaacca ttccataacc gaagccgtta ccgcgcgcct gatgtttttt
    20041 cgcatatcga tagaggtact tccacagcac cggcgtcagg atatatttcg cctcaaccat
    20101 cgggtccagc agctgcgcca gcgtcactcg ctgtgcaggg aaacattcgc tgatatcacg
    20161 cagagtaaaa tcggctttca gattcagatc gcgacgaaaa cccaccagca cgatgcgttc
    20221 acggtgctgc ggcaggaaat gtttaccatc gatgattttc ggatcgtccg gcccgttatc
    20281 ttctgcatcg gcaacgtcat agcccagttc gtccagcgtc tgcatgatga tgcggaacgt
    20341 ttttccctta tcgtgactct tcaggttttt gacgttttcg agcacaaaca tcgccggacg
    20401 acgcgcgtcg ataatgcgca ccacatcaaa gaacagcgtg ccctgggtat cgcaggcaaa
    20461 accgtgcgcc cgcccgagcg agttcttttt cgatacgcca gccagcgaaa atggctgaca
    20521 agggaaaccg gccagtaaaa catcgtgttc aggaatgtgt tgacgaatat gttccgccgc
    20581 cgcctcatca ctcacgcctt cttgatgact gagggtgatg tcgcggatat cttcattaaa
    20641 atgatgcgtc gccgggtcgc aataatggtt ggctttataa gtgcgtaccg catgtttgtt
    20701 ccattcgctg gtaaacacgc actgtccacc aatcgattca aaaccacgac ggatgccgcc
    20761 aattccggca aatagatcga taaagcgaaa cgcataatgc ggatgatgtt ccggtggttt
    20821 gggtaacaac gtttgcagat gggcaaactc tttctcactt aaacggtgcc atgccgagtc
    20881 attcgccagc gcacgtttta aaattgccgc gctccagtga ttctcaccca caccattaag
    20941 ctgcgccacc agcattttaa catcataaat ttgcagcagt ttctccagca ttgcctgtgc
    21001 ggcattcccg gtgctgtatg aatcggttac tgatatattt tcctgcatag atttaaccgg
    21061 ccatctgaga tggccgacag gttaacataa ttactgccgt ttagggccac gcgctcagcc
    21121 agcgggtgaa aacgcatcta tcaccttgtg atcgatacct tcattctccc ccgccagttc
    21181 ggcaccgagc ttcgccatca aacagcgcca agctcaatgc ccctgaaacg gcaaatacgc
    21241 gcgccaggcc aatcgccccc agggcttcca gccgatccgc atcctggacg attttagctt
    21301 ccgttgttaa gggggcgatt tgcgcgctga aactgtgagc ggcaatggca tgacaaacgg
    21361 cctcaatttt ctccgccgga aattgcacga actcttcacg gagcaggcga cgcgtctctt
    21421 ctgccgccag gattgaagaa cgctgccgtt gcggatgatt tttcgccagg ctcacgatat
    21481 cgtgaaaata acaggcggtt aaaatcacca gcatatcgac atcgtcatct gccgccagtt
    21541 tctgtgccgt agcccagaca cggcgaaaat gacacaggtc atgcgccgcg tcctgatgtt
    21601 gatgatggtt tttcaaccag ttctcgaact atgcctgcca gtgttgtaac tccatacgcg
    21661 ctccgttgcc aaagagtctc aaccttagca gtttttattc atagcgtctg caacgtgcgg
    21721 aacgaggtcg ggtgtaccat gcaatgccgc ctaacaaggc accaaccagg gccataaaca
    21781 gaaatccgac caatgctcct aaccacttcc ccgcggtaga tcctaaatca ctttcaaatc
    21841 ccgacacggg ggcatcgggg atccggctca tcacccagat gtatcgcgcc attgcccaga
    21901 cgaaatagcc acaccatgta tagaaagccc
//
LOCUS       DAZSIO010000058        21504 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZSIO010000058 DAZSIO010000000
VERSION     DAZSIO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.058"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="ACHX5T_002002"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="ACHX5T_002002"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR8467209.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="ACHX5T_002003"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="ACHX5T_002003"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR8467210.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="ACHX5T_002004"
     CDS             complement(3121..3420)
                     /locus_tag="ACHX5T_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR8467211.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /gene="wecH"
                     /locus_tag="ACHX5T_002005"
     CDS             3595..4590
                     /gene="wecH"
                     /locus_tag="ACHX5T_002005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR8467212.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /gene="yiaA"
                     /locus_tag="ACHX5T_002006"
     CDS             complement(4632..5069)
                     /gene="yiaA"
                     /locus_tag="ACHX5T_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFR8467213.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /gene="yiaB"
                     /locus_tag="ACHX5T_002007"
     CDS             complement(5115..5456)
                     /gene="yiaB"
                     /locus_tag="ACHX5T_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR8467214.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="ACHX5T_002008"
     CDS             complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="ACHX5T_002008"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFR8467215.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="ACHX5T_002009"
                     /pseudo
     CDS             complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="ACHX5T_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8839..9831
                     /gene="xylF"
                     /locus_tag="ACHX5T_002010"
     CDS             8839..9831
                     /gene="xylF"
                     /locus_tag="ACHX5T_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR8467216.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9909..11450
                     /gene="xylG"
                     /locus_tag="ACHX5T_002011"
     CDS             9909..11450
                     /gene="xylG"
                     /locus_tag="ACHX5T_002011"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFR8467217.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11428..12609
                     /gene="xylH"
                     /locus_tag="ACHX5T_002012"
     CDS             11428..12609
                     /gene="xylH"
                     /locus_tag="ACHX5T_002012"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFR8467218.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12687..13865
                     /gene="xylR"
                     /locus_tag="ACHX5T_002013"
                     /pseudo
     CDS             12687..13865
                     /gene="xylR"
                     /locus_tag="ACHX5T_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
     gene            complement(13973..14797)
                     /locus_tag="ACHX5T_002014"
     CDS             complement(13973..14797)
                     /locus_tag="ACHX5T_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFR8467219.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15117..17147
                     /gene="malS"
                     /locus_tag="ACHX5T_002015"
     CDS             15117..17147
                     /gene="malS"
                     /locus_tag="ACHX5T_002015"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR8467220.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17325..18578
                     /gene="avtA"
                     /locus_tag="ACHX5T_002016"
     CDS             17325..18578
                     /gene="avtA"
                     /locus_tag="ACHX5T_002016"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFR8467221.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18729..19202)
                     /locus_tag="ACHX5T_002017"
     CDS             complement(18729..19202)
                     /locus_tag="ACHX5T_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR8467222.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="ACHX5T_002018"
     CDS             complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="ACHX5T_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFR8467223.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20352..21350
                     /gene="yiaK"
                     /locus_tag="ACHX5T_002019"
     CDS             20352..21350
                     /gene="yiaK"
                     /locus_tag="ACHX5T_002019"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFR8467224.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21362..>21504
                     /locus_tag="ACHX5T_002020"
     CDS             21362..>21504
                     /locus_tag="ACHX5T_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFR8467225.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5403 a         5489 c         5277 g         5335 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaaccct tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 ccccccgtac ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg acgccagtac
     5881 tcactacgcg ccccgccacc aatcaacgta acactttgcg gtttaatgcc gcaggcatgc
     5941 acgacatcca tgccatctgc cagcgcataa cccacgcctt ccagcactgc tcgcgccagt
     6001 tcattggggc catgttgatg agtcaaacca aagaaaaccc ccttcgcctg gggattattg
     6061 tgtggtgtac gctcgccgga aagataaggc agaaaccaaa ctggctcggc actttcatca
     6121 gcctgttgag ctgcagcgat taaagctggg acattgcaca ggccggttaa tttcgcggcc
     6181 caatccagac acgatgctgc actcagcatt acagacatta aatgccaacg ctgcggtagc
     6241 gcatggcaaa agctgtgtac agcgctttct ggcttgctta agaacccttc gctgacagca
     6301 aaatagaccc ccgacgtccc cagcgataac attgcctgat tagcatcaac cattcccaca
     6361 ccaactgcac cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt cgccataccc
     6421 cacgctttcg caacttcagg taacaaagca ccagtaattt cgctgccttc gtataatgcg
     6481 ggcatctggt cacgagataa gtcgcaagcc tgcagcatga cgtcactcca gtcacgcttt
     6541 gcgacatcca gccacatggt gccagctgcg tcagacatat cgctggcaaa ctcccccgtc
     6601 atacgcagac gcaagtaatc tttcggtaat aatactttgt cgatttgacg gaatatctcc
     6661 ggctcatgcc gctgaaccca tagcaattta ggcgcagtaa atccgggcat catcaggttg
     6721 ccggtaatca ctcgtgattg cggaactctc gcttccagca aagtgcactc ttgcgcacag
     6781 cgcccgtcgt tccacaaaat ggcagggcgc aatacccgtt gttgagcatc cagtaaggtt
     6841 gctccatgca tctggccggc aatacccaat gctttaacgt cctgcagaga atgctgatcg
     6901 cccagagctt tcattgcgcg atcagttgcc tgccaccact gttccgggtc ttgttccgac
     6961 caaagtggat gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac cacctcaccc
     7021 tgctcgttga gcaaaataac ttttacgccc gaggtgccaa gatctatccc gatatacata
     7081 tcgatcgttc cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca acggactgca
     7141 cagttagccg ttatttgtcg aacagataat gatttaccag attttccagt tgctcctggc
     7201 gaccactctg atgcaccgga gacaaattat gttcctgagc atatttggct aaatctgcca
     7261 gtgacatttg gcctttcagg atttgctggc ccaattcgct attccagccg gaataacgct
     7321 gcgcgatgcg tttatccagc tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca
     7381 gtgccatcgt atccatcgcg ccgatatgac cgtaaaacag atcatattta tcagtacttt
     7441 gacgacgtac tttggcatcg aagttcagac caccggtggt gaaaccgcct gctttgagaa
     7501 tttcatacat caccagcgca ttctcttcca cactgttcgg gaactggtcg gtgtcccagc
     7561 ccagttgcgc atcgccacgg ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg
     7621 tggctatttc atgatggaaa gagtgacctg ccagcgtcgc gtggttagct tcaatgttca
     7681 gtttaatctc tttttccaga ccaaactgtt tcaggaagcc atagaccgtc gcggcatcgt
     7741 aatcatattg atgtttagtc ggttcttgcg gtttcggttc gataagcaac gtgccctaga
     7801 agccgatttt atgtttatgc tcaaccacca tctgcataaa gcggcccagt tgttcacgct
     7861 cctgacgcaa gtcggtattt aacagcgttt cgtaaccttc acgaccaccc cacaggacat
     7921 agttttcacc gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc
     7981 agctgaagac ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa
     8041 agcagttagc ggttccccac agcagcttca cgctgctctc ttcttgcttg gctgccagga
     8101 catcaaccat ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa
     8161 catccacatc gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga
     8221 catctgcttt acgcttcgcc aacgccagtg cctcaccagg ctgctgccac ggacgattaa
     8281 acgcccccac accaaacatg tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg
     8341 caaaacgcaa gtgctcttcc atacgcttac ccaacaccag ttcgtcggga ttgtagtgac
     8401 ggaatgctaa cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa
     8461 aataggcttg catattgaac tccataatca ggtaatgccg cgggtgatgg atgatgtcgt
     8521 aatattgggc actccctttc aattgctcaa ttatgttatt tcacactgct attgagataa
     8581 ttcacaagtg tgcgctcgct cgcaaaataa aatgggatga tgaaaatggg taattcctcg
     8641 aagataaaaa tgcaataagt acaattgcgc aacaaaagta agatctcggt cataaatcaa
     8701 gaaataaacc aaaaatcgta atcgaaagat aaaaatctgt aattgttttc ccctgtttag
     8761 ttgctaaaaa ttggttacgt ttatcgcggt gattgttact tattaaagct gtcctctaac
     8821 tacagaaggc cctacaccat gaaaataaag aacattctac tcaccctttg cacctcactt
     8881 ctgcttacca acgttgcggc acacgccaaa gaagtcaaaa taggtatggc gattgatgat
     8941 ctccgtcttg aacgctggca aaaagatcga gatatttttg tgaaaaaggc agaatctctc
     9001 ggtgcgaaag tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt
     9061 gaaaacatga ttaaccgggg tgtcgatgtt cttgtcatta ttccgtataa cggtcaggta
     9121 ttaagtaacg ttgtaaaaaa agccaaacaa gaaggtatta aagtattagc ttacgaccgt
     9181 atgattaacg atgcggatat cgatttttat atttctttcg ataacgaaaa agtcggtgaa
     9241 ctgcaggcaa aagccttggt cgatattgtt ccgcaaggta attacttcct gatgggcggc
     9301 tcgccggtag ataacaacgc caagctgttc cgcgccggac aaatgaaagt gttaaaacct
     9361 tacgttgatt ctggaaaaat taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg
     9421 gaaaacgcat tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa aattgatgct
     9481 gtagttgcct caaacgatgc caccgcaggt ggggcaattc aggcattaag cgcgcaaggt
     9541 ttatcaggga aagtagcaat ttccggccag gatgcggatc tcgcaggtat taaacgtatt
     9601 gctgccggta cgcaaactat gacggtgtat aaacctatta cattgctggc aaatactgcc
     9661 gcagaaattg ccgttgaatt gggcaatggt caggagccaa aagcagatac ctcactgaat
     9721 aatggcctga aagatgtccc ctcccgcctc ctgacaccga tcgatgtgaa taaaaacaac
     9781 atcaaagata cggtaattaa agacggattc cacaaagaga gcgagctgta agcgttacgc
     9841 cccggcgcgg agcgggggcg tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg
     9901 gagtcgttat gccttatcta cttgaaatga agaacattac caaaaccttc ggcagtgtga
     9961 aggcgattga taacgtcagc ttgcggttga atgctggcga aatcgtctca ctctgtgggg
    10021 aaaatgggtc tggtaaatca acgctgatga aagtgctgtg tggtatttat ccccatggct
    10081 cctacgaagg cgaaattatt tttgcgggag aagagattca ggcgagtcac atccgcgata
    10141 ccgaacgcaa aggtatcgcc atcattcacc aggaattggc cctggtgaaa gaattgaccg
    10201 tgctggaaaa tatcttcctg ggtaacgaaa taacccacaa tggcattatg gattatgacc
    10261 tgatgacgct acgctatcag aagctgctcg cacaggtcag tttatccatt tcacctgata
    10321 cccgcgttgg cgatttaggg cttgggcaac aacaactggt tgaaattgcc aaggcactta
    10381 ataaacaggt gcgcttgtta attctcgatg aaccgacagc ctcattaact gagcaggaaa
    10441 cgtcggtttt actggatatt attcgcgatc tacaacagca cggtatcgcc tgtatttata
    10501 tttcgcacaa actcaacgaa gtcaaagcga tttccgatac gatttgcgtt attcgcgacg
    10561 gacagcacat tggtacgcgt gatgctgccg gaatgagtga agacgatatt atcaccatga
    10621 tggtcgagcg agagttaacc gcgatttacc ctaatgaacc acataccacc ggagatgaaa
    10681 tattacgtat tgaacatctg acggcatggc atccggtcaa tcgtcatatt aaacgagtta
    10741 atgatgtctc gttttccctg aaacgtggcg aaatactggg tattgccgga ctcgttggtg
    10801 ccggacgtac cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa
    10861 aaatttatat tgatggcaaa caggtagata ttcgtaactg tcagcaagcc atcacccagg
    10921 ggattgcgat ggtacccgaa gacagaaagc gcgacggcat cgttccggta atggcggttg
    10981 gtaaaaatat taccctcgcc gcactcaata aatttaccgg tggcattagc cagcttgatg
    11041 acgcggcaga gcaaaaatgt attctggaat caattcagca actcaaagtt aaaacgtcgt
    11101 cccccgacct tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg atcctcgccc
    11161 gctgtctgtt acttaacccg cgcatcctca ttcttgatga acctaccagg ggtattgata
    11221 ttggcgcgaa atatgagatc tacaaattaa ttaaccaact cgtccagcag ggtattgccg
    11281 ttattgtcat ctcttccgaa ttacctgaag tgctcggcct tagcgatcgt gtactggtga
    11341 tgcatgaagg gaaactaaaa gccaacctga taaatcataa cctgactcag gagcaggtga
    11401 tggaagccgc attgaggagc gaacatcatg tcgaaaagca atccgtctga agtgaaattg
    11461 gccgtaccga catccggtgg cttttctggg ctgaaatcac tgaatttgca ggtcttcgtg
    11521 atgattgcag ctatcatcgc aatcatgctg ttctttacct ggaccaccga tggtgcctac
    11581 ttaagcgccc gtaacgtctc caacctgtta cgccagaccg cgattaccgg catcctcgcg
    11641 gtaggaatgg tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg
    11701 gggctattag gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc tttgccactt
    11761 accatcattg tgacgttggt tctgggactg cttctcggtg cctggaacgg atggtgggtc
    11821 gcgtaccgca aagtcccttc atttattgtc accctcgcgg gcatgttggc atttcgcggc
    11881 atactcattg gcatcaccaa cggcacgact gtatccccca ccagcgccgc gatgtcacaa
    11941 attgggcaaa gctatctccc cgccagtacc ggcttcatca ttggcgcgct tggcttaatg
    12001 gcttttgttg gttggcaatg gcgcggaaga atgcgccgtc aggctttggg tttacagtct
    12061 ccggcctcta ccgcagtagt cggtcgccaa gctttaaccg ctatcatcgt attaggcgca
    12121 atctggctgt tgaatgatta ccgtggcgtt cccactcctg ttctgctgct gacgttgctg
    12181 ttactcggcg gaatgtttat ggcaacgcgg acggcatttg gacgacgcat ttatgccatc
    12241 ggcggcaatc tggaagcagc acgtctctcc gggattaacg ttgaacgcac caaacttgcc
    12301 gtgttcgcga ttaacggatt aatggtagcc atcgccggat taatccttag ttctcgactt
    12361 ggcgctggtt caccttctgc gggaaatatc gccgaactgg acgcaattgc agcatgcgtg
    12421 attggcggca ccagcctggc tggcggtgtg ggaagcgttg ccggagcagt aatgggggca
    12481 tttatcatgg cttcactgga taacggcatg agtatgatgg atgtaccgac cttctggcag
    12541 tatatcgtta aaggtgcgat tctgttgctg gcagtatgga tggactccgc aaccaaacgc
    12601 cgttcttgat tttgataaaa attttctcaa agccggttac gtattaccgg ttttgagttt
    12661 ttgcatgatt cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc acattactgt
    12721 tcaatgccaa taaagcctat gaccggcagg tagtagaagg cgtaggggaa tatttacagg
    12781 catcacaatc agaatgggat attttcattg aagaagattt ccgcgcccgc attgataaaa
    12841 tcaaggactg gttaggagat gtcgtcattg ccgacttcga cgacaaacag atcgagcaag
    12901 cgctggctga tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac cttgcagaaa
    12961 gttacccacc cgttcattac attgccaccg ataactatgc gctggttgaa agcgcatttt
    13021 tgcatttaaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg
    13081 gcaaacgttg ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt
    13141 atcgcggagt ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa
    13201 atcggctggc agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg
    13261 acgcccgagc gcggcatatt ctgcaagtat gtgaacatct acatattccc gtaccggaaa
    13321 aattatgcgt gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc
    13381 tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc
    13441 gattattaga taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca
    13501 ttgaacggcg ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc
    13561 attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg
    13621 ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg
    13681 caatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt
    13741 cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt
    13801 ttaaaaaagc atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt
    13861 tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc
    13921 gagcaaaagc cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat
    13981 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
    14041 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
    14101 ccgctttacg cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca
    14161 ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca
    14221 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat
    14281 tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
    14341 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
    14401 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
    14461 gtgcccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg
    14521 cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc
    14581 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
    14641 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
    14701 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
    14761 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
    14821 cattccaaca ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa
    14881 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga
    14941 ttcagattgg cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt
    15001 gcgcgcgatc gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
    15061 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga
    15121 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
    15181 ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt
    15241 tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag
    15301 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
    15361 ggcgattgtt cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa
    15421 cattgatgat ttccatccag aacgccgccg aaccggtagc aaacctggtc cgtgaatgcc
    15481 cgaaatggga tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg
    15541 ccgtacggga ttattacagc caacaaattg cgatagtgaa gaacggtcaa ataacgttac
    15601 aacccgctgc taccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg
    15661 cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg
    15721 gcgatcccag taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca
    15781 cttttcacgg cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg
    15841 gcgttaatgc tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggtg
    15901 gtacaaaagg cgatttcccg cattatgcct accacggtta ttacacacag gactggacga
    15961 atcttgatgc caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc
    16021 agcgcggtat tcgtattctc tttgatgtcg tgatgaacca caccagctat gccacgctgg
    16081 cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa
    16141 cgctgggtga acgctggagc gactggaaac ctgccgccgg acaaacctgg catagcttta
    16201 acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga
    16261 tcagaacgga tatcggcgat tacgacaatc ctggattcga tgatctcact atgtcgctgg
    16321 cctttttgcc ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata
    16381 aaaacaaaac ggatactcac gctaaagtca tcgaaggctt tacacctcgc gattacttaa
    16441 cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg
    16501 ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc tccgcgcttc
    16561 gcgaatggaa aaaaactaac cccgacaaag cattagatga caaacctttc tggatgaccg
    16621 gtgaagcctg gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga
    16681 tgatcaattt cgattatcag gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg
    16741 atacgacctg gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct
    16801 cgtcgcatga tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat
    16861 tagcgccagg cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta
    16921 caggttctga tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta
    16981 aatctgccgc caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg
    17041 caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg
    17101 agcatggcga cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc
    17161 ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt
    17221 tgcgggataa agcaccgctc actcttcaac agaccgattt gcaccccagc aaatgtagcg
    17281 ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt
    17341 ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg
    17401 gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa
    17461 tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg
    17521 cactgtgtaa ctacgacggt ccacagggga aaacggagct actcacactg cttgccggaa
    17581 tgctgcgcga gaagttgggt tgggatatcg aaccacagaa tattgcacta acaaacggca
    17641 gccagagcgc gtttttctac ttatttaacc tgtttgccgg acgccgtgcc gatggtcggg
    17701 tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg
    17761 aagaagatct gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta
    17821 aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct
    17881 cccggccgac gaatccaaca ggtaatgtga ttactgacga agagttgctg aagcttgacg
    17941 cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc
    18001 cgggtatcat cttcagtgaa gggcgcccgc tatggaatcc gaatatcgta ctgtgcatga
    18061 gtctttccaa gctggggctg cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa
    18121 tcatcactgc catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc
    18181 cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca
    18241 tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac
    18301 cggaaaatcg ctgcctgatt cataaaccgg aaggagccat tttcctctgg ctatggttta
    18361 aggatttgcc cattacgacc gagcagctct atcagcgcct gaaagcacgc ggcgtgctga
    18421 tggtgccggg gcacaacttc ttcccgggac tggataaacc gtggccgcat acgcatcaat
    18481 gtatgcgcat gaactacgta ccagagccgg agaaaattga ggcgggggtg aagattctgg
    18541 cggaagagat agaaagagcc tgggctgaaa gtcactaaaa attgcctgat gcgctacgct
    18601 tatcaggcct acatgatttc tgcaatatat tgaatttgga agaatttgta ggccggataa
    18661 ggcgtttacg ccgcatctgg cataaacaaa gcgcactatt ccggcattga gaaacgccgg
    18721 aaaacgtttc aaacaggctg ctgctgtagc cgatgccgct gcactttctc gacatccatg
    18781 cactgcaacg cctttgtcgg gcaggcttca acacaggccg gccccgtctc ccgatgccag
    18841 cacagatcgc acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca
    18901 aacgggcaag ccagcataca gcttttacag ccaatgcaac gtgtttgttc aacgaaaata
    18961 tgcccatgtt cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca
    19021 cactgatgac aggctactgg cgtggtccag ctgtggtctt taatgacacg aatacgggaa
    19081 ataaactcgt ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa
    19141 gccacttcac aggtacggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc
    19201 atgatcattc ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga
    19261 tccaacgaag gaaatgttaa agttttggca cttgtccaaa gcgttatgtg atcgcgccca
    19321 ggtcatccct gacagtaaat cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg
    19381 gtttcaggag atttttctct cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca
    19441 cggcgtacgg cacccgccca tgaatatcaa acaccggaac agcaatacag gagacgccga
    19501 gttcgttttc ttctctgtcc atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga
    19561 acatcgcggg cagctcggta atggtattgc gggttaacgg ctggatctca tgctgatggt
    19621 tttcccagta tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg
    19681 cggaacagta gagcggcata tgctggccaa tataggcacg ggttcgcagc atcccggttg
    19741 tgggttccag cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg
    19801 tttcaccagt ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca
    19861 gcgaagacag cgccttctgc ccgacggcaa taaatttggt agtcaggcgg taactccctg
    19921 cggcgggcgc ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg
    19981 tactcttatt taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt
    20041 tgctcaaaat ctcaatcagc atcaacccgc gaaacagact ctggcttccg gctggacgct
    20101 ctttttcctg cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc
    20161 gtcctgatgg tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca
    20221 cttgattttt atgtcttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat
    20281 atatttgaaa tctagttccg catattgaaa ttttaagcca aaaaagcgat caaaaaacaa
    20341 ggaagcctag gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa
    20401 tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca
    20461 ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg
    20521 aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg
    20581 aacagtggga tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg
    20641 ccattgaact ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact
    20701 ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa aggctatatt ggcatttgct
    20761 ggaccaactc catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc atcggcacta
    20821 acccgctgat cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg tcgatgtcga
    20881 tgttctctta cggcatgtta gaagttaacc gcctggcagg ccgtcagctc ccggtcgatg
    20941 gtggctttga tgatgagggc aatttgacca aagaacctgg cgttatcgag aagaatcgcc
    21001 gcattttgcc gatgggctac tggaaaggtt ctggcatgtc gattgtgctg gatatgatcg
    21061 ctactctcct ttccgacggc gcatcggttg ccgaagtcac ccaggacaac agcgacgaat
    21121 acggcatttc acaaattttt attgccattg aagtggacaa gcttatcgac ggtcccaccc
    21181 gcgatgccaa gctgcaacgt atcatggatt acgttactac cgctgaacgc gctgacgaaa
    21241 accaggccat ccgcttaccc ggccacgaat ttactaccct gctggccgaa aaccgccgta
    21301 acggcatcac tgttgatgac agcgtgtggg ccaaaatcca ggcgttatga ggagataagt
    21361 catgattttt ggacatatcg cgcagcccaa tccgtgccgt ttgcccgccg ccattgaaaa
    21421 ggcgctcgat tttctgcgcg ctaccaactt caacgtcctg gagccgggcg ttgtcgaaat
    21481 cgacggcaag aatatttatg cgca
//
LOCUS       DAZSIO010000059        21277 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZSIO010000059 DAZSIO010000000
VERSION     DAZSIO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.059"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..446)
                     /locus_tag="ACHX5T_002021"
     CDS             complement(<1..446)
                     /locus_tag="ACHX5T_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR8467226.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(501..1352)
                     /gene="manZ"
                     /locus_tag="ACHX5T_002022"
     CDS             complement(501..1352)
                     /gene="manZ"
                     /locus_tag="ACHX5T_002022"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR8467227.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1365..2165)
                     /gene="manY"
                     /locus_tag="ACHX5T_002023"
     CDS             complement(1365..2165)
                     /gene="manY"
                     /locus_tag="ACHX5T_002023"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR8467228.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2228..3199)
                     /gene="manX"
                     /locus_tag="ACHX5T_002024"
     CDS             complement(2228..3199)
                     /gene="manX"
                     /locus_tag="ACHX5T_002024"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR8467229.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3516..3674
                     /gene="yoaL"
                     /locus_tag="ACHX5T_002025"
     CDS             3516..3674
                     /gene="yoaL"
                     /locus_tag="ACHX5T_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR8467230.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            3662..5212
                     /gene="yoaE"
                     /locus_tag="ACHX5T_002026"
     CDS             3662..5212
                     /gene="yoaE"
                     /locus_tag="ACHX5T_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR8467231.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5216..6814)
                     /locus_tag="ACHX5T_002027"
     CDS             complement(5216..6814)
                     /locus_tag="ACHX5T_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR8467232.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6945..8309)
                     /gene="sdaA"
                     /locus_tag="ACHX5T_002028"
     CDS             complement(6945..8309)
                     /gene="sdaA"
                     /locus_tag="ACHX5T_002028"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR8467233.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8493..9071)
                     /locus_tag="ACHX5T_002029"
     CDS             complement(8493..9071)
                     /locus_tag="ACHX5T_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR8467234.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(9075..10436)
                     /gene="pabB"
                     /locus_tag="ACHX5T_002030"
     CDS             complement(9075..10436)
                     /gene="pabB"
                     /locus_tag="ACHX5T_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR8467235.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10510..10689
                     /locus_tag="ACHX5T_002031"
     CDS             10510..10689
                     /locus_tag="ACHX5T_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR8467236.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10809..11108)
                     /locus_tag="ACHX5T_002032"
     CDS             complement(10809..11108)
                     /locus_tag="ACHX5T_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR8467237.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(11530..11874)
                     /locus_tag="ACHX5T_002033"
     CDS             complement(11530..11874)
                     /locus_tag="ACHX5T_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR8467238.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12006..13916
                     /locus_tag="ACHX5T_002034"
     CDS             12006..13916
                     /locus_tag="ACHX5T_002034"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFR8467239.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13974..14669
                     /gene="tsaB"
                     /locus_tag="ACHX5T_002035"
     CDS             13974..14669
                     /gene="tsaB"
                     /locus_tag="ACHX5T_002035"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR8467240.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14709..15290
                     /gene="yeaY"
                     /locus_tag="ACHX5T_002036"
     CDS             14709..15290
                     /gene="yeaY"
                     /locus_tag="ACHX5T_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR8467241.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15496..17181
                     /gene="fadD"
                     /locus_tag="ACHX5T_002037"
     CDS             15496..17181
                     /gene="fadD"
                     /locus_tag="ACHX5T_002037"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR8467242.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17263..18378
                     /gene="rnd"
                     /locus_tag="ACHX5T_002038"
     CDS             17263..18378
                     /gene="rnd"
                     /locus_tag="ACHX5T_002038"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR8467243.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18432..19397)
                     /gene="yeaX"
                     /locus_tag="ACHX5T_002039"
     CDS             complement(18432..19397)
                     /gene="yeaX"
                     /locus_tag="ACHX5T_002039"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR8467244.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19453..20070)
                     /gene="yeaW"
                     /locus_tag="ACHX5T_002040"
                     /pseudo
     CDS             complement(19453..20070)
                     /gene="yeaW"
                     /locus_tag="ACHX5T_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
     gene            complement(20412..21020)
                     /locus_tag="ACHX5T_002041"
     CDS             complement(20412..21020)
                     /locus_tag="ACHX5T_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8467245.1"
                     /translation="MTGEKESKNAERIFMPPEIMRFKERLEEAMNGESSRAFAAKCGL
                     SDGVIRNYLSGKTYPSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDR
                     STLKTKLIAIVELLEDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQ
                     TAKMLESLPTDTRKKILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21190..>21277
                     /locus_tag="ACHX5T_002042"
     CDS             21190..>21277
                     /locus_tag="ACHX5T_002042"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8467246.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5266 a         5274 c         5465 g         5272 t
ORIGIN      
        1 acgagaagct tataaatttt ttccagatcg tcgatatttc gaacgcggat taacagccga
       61 cgctgttcta attgcatcac caacacgcca tcttccgaca agttcatcgc tttgattcgg
      121 ctatattcaa tccagacatt ggcgaagaaa aaaccttttt gtttaaagat gatcttcgga
      181 atgcggatcc agaatatata aaaacccatc agagccagtg cacttaataa ccatgttgtt
      241 attagcgcgc catgattcgt gacgttgtta tagataagaa tgacaatcaa tccgacgaag
      301 ataacgctat cgatgcgacc acgccggagc aaaggaattg ccagcagggt ggggccgtta
      361 cggcggggca tgatgaactg atcgtagatc gcgaaggcca ggagtgcggc gatgaaaaga
      421 atcagtacca ggtccgtgat tgtcattaat cctccagata aaaaaacggg gccaaaaggc
      481 cccggtagtg tacgacagtc ttacagtccc agcaggccgc aagcgtaacc agcgataccg
      541 atgacgaaga agccaacgat gatccacagc gggttaactt ttttgcgcag cagccacata
      601 caagcaaatg tcagcagcag tggtacaagg cctggcatta actggtcaag aatagtttgc
      661 acggtggtga cacgggtctg tccatcctgc ccggtgatgg tcgaaaccac cagcgggatg
      721 ttgacatgtg tccacttgtt aaccaatgcc cccatgacaa acaggccgag gatagacgcc
      781 ccttccgtca gtttttgcag gaagccacca cccatatctt taacgatatc gatacctttg
      841 gagtaaccat acgctacgcc gtagtaacgg gttgccagac gcaccaggtt aaacaggatg
      901 aagaacagca gcggacctaa caggctgccg ctcatcgcga taccggcacc cagtgctgca
      961 aataccggac gtacggttcc ccagaagatc gggtcgccta caccagccag cggccccatc
     1021 aaaccgactt tgataccgtt gatagcaccg tcatcgatct ctgcgccatt agcacgctgt
     1081 tcttccagcg ccagggttac gccgagaatc ggtgcagcca cgaacggctg ggtgttaaag
     1141 aactccaggt gacggcgaat agcttgttta cgagcttcgt tgttctcagg gtagaggcga
     1201 cgaattgccg gtaccataga gaagcagaaa cccagtgcct gcatacgttc gaagttccat
     1261 gaaccctgga agaggttaga acgcaggaat acgccacgaa tatcactttg agtgagtttt
     1321 ttctcggtgg tagtttgagt tgtatcaacc atttcgctca cctgttagtc cagttcgtta
     1381 tcgagatcgt tgttaccagc tgcctgagca ggcgcaccgg ctacgcggtt gtatttcggg
     1441 ctaagttgga tgtagagcac tgccataaca gtaccaatca cacccagagc aaccaggtta
     1501 aagttggtga atgctgcggt tacgaagccg aggtagaaga acggcatcag gtagccagca
     1561 cgcatcatgt tgataaccat cgcataacca accaccacga tcatgccccc ggcgatattc
     1621 agaccattgg tcaccacttc cggaatcgca ttcagcatgt tctgtacttc gctggtacca
     1681 acagacagcg caacgatgac ggccggaata gccacacgca ttgcttgcag gaacagagaa
     1741 gaaacgtgga tccaggaaat cgctgtcagg ttgccgttat cagcagcctt atccgcagcg
     1801 tgctggaaag caacggtaat agtacgaacg atgatggtca gtacctggcc cgcagcggcc
     1861 agagggattg ccagtgcgat acctgcacca atgctctgat gacctgcgat aaccagaatg
     1921 gtagaaatga tagaagccag agcggcgtca ggcgcaactg cagcaccgat gttcatccag
     1981 cccagcgcga tcatttccag cgtaccaccg ataataatac cggttttcat atccccaaga
     2041 acgataccca ccagggtaca cgcgattagc ggacggtgaa actgaaattc atcgaggatt
     2101 gatcccatac ctgcgataca ggctacgata aataccagca caatttgaag agtggtaatc
     2161 tccattgtac ttctcctgtt tacgtaagac ttaagtgtga aaactgaatg ataatcaaca
     2221 caatacgtta cttatcgatt ttgctgatca gatccatcat tttcagtttc ggatcggtgg
     2281 aaaccttacg gacttccagc tcaataccgc gtgcattcag ttttttgaac gcctcgatat
     2341 ctttttcatc aaccgaaacc gcgttattca cctgggtttt accctgacgg aatgccatac
     2401 caccgacgtt aacagaggtg attttcacgc cgccttcaac gagacgctct acatctgttg
     2461 ggttggtaaa taacagcatt acgcgttcgc cagcatattt cgggttgttg tagacgcgaa
     2521 tcattttggc aacatcaact acgtgtgctg ttacgcccgg aggtgcaacc tgggtgagca
     2581 gtgtcttacg aacggtatcc gcagccactt catcactaac aacaataata cgggagacat
     2641 tggtttcttt ggtccagcgg gtggcgacct gaccgtgaat cagacggtcg tcgatacgcg
     2701 caaggccaat aaccatgtag tcgtttggcc ccattggttt tgccggagtt ggagccgctt
     2761 ttggtgctgc ggcagcgggt gctggcgcgg ctttttcaac cggtttggct ttcagtgctt
     2821 tcacgccttc acggcctgtt tctactgcca gcgcaaccag ttcatcaaag ctcgggtcat
     2881 catcacgggc cattaacgtt tccacgagca ttggaatgtt aacgcctgca atgacttcat
     2941 aatgctcttt gtcgacgaca atgcggctgg cagcattgaa cgggctgcct ccccatgtat
     3001 caacgagaaa cagcacgcct ttagtggtgt cgagttttgc caactgagcg ttgtattttt
     3061 caatcagcgt ttcggcattt tcacctggaa cgaaatcgat ccagccgacg ttttcctgct
     3121 cgcctaacag catttctgcc gttttaagca actgctctgc agcccaacca tgtgtgccta
     3181 taacaatagc aatggtcact tgctacctcc tttattatcg ttaacacctc aacgtgccag
     3241 atgtattttt gaatcgtcgt ccacaatcga atcgattcag ataagggcta caaccaaaaa
     3301 cccctgcgtt tcgcgattta ttttagatat cgaaaaaatt attttatgtg atgaagatcc
     3361 gtaatttaac tttcgattat cagaaatttc gaaaggtaaa atatccttgt cacattcgtt
     3421 tgcaaaggaa ggtaaatctt tgccaaatca gaggcgtctc tgatatgttt aactcccgtt
     3481 taacaaccat ggagtatagg gcagtagccc gcagtatgga tcgtcaccga cgtcatttca
     3541 gcatcaggcc ttttaacgcc tgcctttctg gcactctatg ccgcaccttt cgtttgcatt
     3601 ttgtcgttac gcctgcatta tttctggcgt cgaatagcta ttccttaagc aggagcttgt
     3661 catggaattc ttaatggacc cctcaatttg ggcggggcta ctcacgcttg ttgttctcga
     3721 aattgtgctg ggtatcgata acctggtctt catcgccatt cttgctgaca aactgccgcc
     3781 aaaacaacgc gataaagcgc gtttgctggg gttatcactg gcgctgatta tgcgtctggg
     3841 gctgctgtcg ctgatttcat ggatggtcac gctgaccaaa ccgctattta ccgtcatgga
     3901 tttctccttc tccggacgcg atctgattat gttgttcggg gggatattct tgctgttcaa
     3961 agcaacaacc gaactgcatg aacggctgga aaaccgcgat catgattccg gccacggtaa
     4021 aggctacgcc agtttctggg tggtcgtcac acagattgtc atccttgacg ccgtcttctc
     4081 gttggatgcg gtaattactg cagtagggat ggttaaccat ctgccggtga tgatggcggc
     4141 ggtagtgatt gcgatggcgg ttatgttgct ggcatcgaaa ccgctgacgc gattcgttaa
     4201 ccagcacccc acggtggtgg tgctctgtct gagcttcctg ttaatgattg gtctgagtct
     4261 ggtggcagaa ggtttcggtt tccacattcc gaaaggttac ctgtatgccg cgattggctt
     4321 ctcgatcatt atcgaagtgt ttaaccagat tgcgcgtcgc aactttattc gtcaccagtc
     4381 gactttgccg ctgcgagcgc gtactgccga tgccatcctg cgtttgatgg gcgggaaacg
     4441 tcaggccaat gttcagcacg atgccgataa cccgatgccg atcccggaag gtgcatttgc
     4501 cgaagaagaa cggtacatga ttaacggcgt actgacgctg gcgtcgcgtt ctctgcgcgg
     4561 gatcatgacg ccgcgcggtg aaataagctg ggttgacgct aatctcgggg tcgatgaaat
     4621 ccgcgagcaa ctgctctctt caccgcacag tctgttcccg gtatgtcgcg gtgaactgga
     4681 tgaaatcatc ggtatcgtac gtgctaaaga actgctggtg gcgctggaag agggcgttga
     4741 tgtggcggcg attgcttcgg cgtctccggc gattatcgtc ccggaaaccc tcgatccgat
     4801 caacctgctg ggcgtgctgc gtcgcgctcg cgggagcttt gttatcgtga ccaacgagtt
     4861 tggtgtggta caaggcttgg tcacgccgct ggatgtgctg gaagccattg cgggtgaatt
     4921 cccggacgct gacgaaacgc cggaaatcat taccgatggt aacggctggc tggtaaaagg
     4981 cggtacagat ttgcatgcct tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga
     5041 tgatatcgcg acggtcgcgg gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg
     5101 cgatgtgatt gatgtagggc cactgcatat caccatcttt gaagccaatg attatcgtgt
     5161 tgatctggtt cgcattgtta aagagcaacc ggcgcacgat gaagatgagt aagcgttaac
     5221 gtaacgacat aatgggcgtg atatgtccat tatgccgggc gggcggcggt tggctgcccg
     5281 ccagccattt gggaaaatca cgtagcggca tcggctgtgc gtacaaatac ccttgcaaaa
     5341 tatgcacgcc atgacggcgc aaatacttcg cctgttcttg cgtttccacg ccctccgcca
     5401 ccagttcaat attcagtctt tgccccagcg cgatgatgat atcggtcacc gtcgagttaa
     5461 ccgcgtcaga acctatagct gcggtaaatg acttatcaat tttcagcacg tcaggacgta
     5521 atgtttcaag ccaggaaaac gagctgttgc cggtgccgaa gtcatcaatc gccagtttga
     5581 cgtttttacg atgcagctcg cgggcaatcc gataatcaac atccagtaag gcatcgcgtt
     5641 cggtgatttc gaggatcagt tgctgaatcg ggtgagcgct aaaccagtac tgattgagat
     5701 cttttatcag cacaccacgg cgaaaatggc tgggtgcgac gttaatgcca acatgaaact
     5761 gactactcat cgggaaaaca tggtgctgac gaatggtttc tgccatcaca tagtgggtca
     5821 gtggcacaat taaatgatgt tcttccgcga taggaataaa cacatccggt gaaatccagc
     5881 cctgacgcgg attattccag cgcagcaaaa tctctacacc aatacattgc tggctgcgcg
     5941 cattaagcaa aggctgacag aacaattcga actcatgttg cgccaggccc agattaattt
     6001 cgcgggaaaa gctcatccgg taagcggtcg ccagccaggc gatatagccc accagtaaac
     6061 ttagcagcac cgccagcggt aattgtgttg gaagatagtg ccatgccagc gccgtagccc
     6121 ccggaccatt tacgttaatg gtaaaaggaa agctttgcga agaaacctga tagttttcat
     6181 tgtcttcagg ttgcggaagg gaatctacca gcccattacc atagagcaaa tgccgtttgt
     6241 ccaccgtcag gctggcgcta ctgatttgcg gcagttgtgg ctcaaggagc attgccgtca
     6301 gtaagtcgat gttaatcatc tctatgaccc cagcattgct actgcccgct gcaggcgtcc
     6361 attgaatcaa aaccggactg cctttaatca gggcacggtc gatcgtcagg cgtaaaagtg
     6421 gttgcggtgc aggaagttca gccagaatgt ccacgacggg cacattgcga taaccaaaaa
     6481 tgctggagca ataaagtgtg ccgtcttgca ccaggccaat ggatcgcaca gtttggagtt
     6541 ttgccgcctg tttacgtaat ggcaaatggg cgacagagca gggcagacca atcagcggaa
     6601 gcaagacttt gctacctgcc tgtaggggaa gcagtacttt atctaattcc tctacagcgt
     6661 gattggcgaa ttgtactacc cgttgttgat ttaagttacg ctgtgaaata aatcgcacgc
     6721 tcagggtcga agcgagcgta acgagtgcgc aaatcgtaca aacaatcatt cgattacggc
     6781 gataggtttt aatgatccgt tgtgcttttt gcatgcgggc caccctgtaa gcgtatggcg
     6841 acaaacgcct gacgcaacag tggaagtgta gtggggaata ttacagcaga cgagaaagcg
     6901 ggtataaatt cgcccatccg ttgcagatgg gcgagtaaga agtattagtc acactggact
     6961 ttgattgcca gaccaccgcg tgaggtttcg cggtatttgg cgttcatgtc cttaccggtt
     7021 tcgtacatcg tttcgatgac cttatccagc gagacgcgcg gtgcactggt gcggcgcaga
     7081 gccatccgtg cggcgttaat cgccttcaca gaggcaatgg cattacgctc aatgcacggc
     7141 acctgaacct gccctgcaac cgggtcgcag gttaaaccaa ggttgtgttc catgccaatt
     7201 tccgccgcca cgcaaacctg ttccgggcta ccgcccagca gttctgcaag acccgcagca
     7261 gccattgaac aggcaacacc cacttcgccc tggcaaccaa cttccgcacc ggaaatagag
     7321 gcgttcattt tatacaatgc accaatcgcg cccgctgcca taaagtaacg ggtatagatg
     7381 tctgggctga ccgattcaat aaagtggtca tagtaagcca gcactgccgg aacgataccg
     7441 caggcaccgt tggttggcgc agttaccaca cgaccaccgg cggcgttttc ttcgttaact
     7501 gccagcgcaa acatgtttac ccagtcaatg acattcatcg gatcgttaga cagtttatcg
     7561 ctggaaacta acatccggcg cagggcagac gcacgacgtg gcacgcgcag cgggcctggc
     7621 agtacacctt cggtgttcat cccgcgatcg atacatgcct gcatggtttg ccagacatgc
     7681 gcgaaatact cgtcgatctc tttcttgctg tgcagcgcca gttcgttctg catagcgaga
     7741 ccagacagcg aatagccggt ttcattacag tacgcgagca gttcggtggc agatttgaac
     7801 ggatacggca cgctcacttc gttggcagca tcctgaccaa agtgttcttc atcgacgata
     7861 aaaccgccgc cgatggaata ataagttttg ctgtagacga cttcatcgcc gttataggcg
     7921 tggatttgca taccgttttc atgcagcggc aggttgccgt tatgaaaacg catcccgttg
     7981 tcgcgcggga aatccacttc atgccgtccc tgtgccagca gcagacgttc gcgctctttt
     8041 acgtcgcgaa taaaaccggg aatactgtcg atatccacgg tggcaggttc gttacctgca
     8101 agacccataa taatggcgat atcggtgtgg tggcctttac ccgtcagcga cagtgaacca
     8161 taaacgtcca cggcaacgcg agtaacgcta tccagtaagc ctttttcgac cagatcatcg
     8221 acgaactgtt tacccgcctt cataggccct acggtatggg aagatgaggg accaatcccc
     8281 accttaaaca tgtcgaatag actaatcacg ataatactcc tgacaaggtg actggacttc
     8341 cagtaacgaa ctaataacag cgcatagtgt aagagggaac gcggcgactg gcttaactat
     8401 tcacatgaat taaactaatc aaaaaccgcg atttaactaa tattgcgatg attgtctcaa
     8461 gtagtgtttt gtagatgtaa agataagtat agtcaggggg tcacaccaat ttgcagcgcc
     8521 agctcacgaa ttatgcctgc ggtcattccc catacaaaat actgttcgta ccaggacagc
     8581 catacccgat gtgaatcgcc acggcggtag atatctaaag ggtgataacg acccagatgt
     8641 aatgcctggg cgagcggcat ttcaaacacc gccgagactt catcttcact ggcgcgatac
     8701 ggcagatcgg gcgggataat gccgaccact ggggttactt gatagccagt tacgctatcg
     8761 acgggcggca gcacgccgat aacttcaacg gcggaaggcg gtatagcgac ctcttcttca
     8821 gcttcgcgca gcgcggcggc gataactgat gcgtccgtgt catcgactgc acctccaggg
     8881 aatgccactt gtccagcgtg tttacgcaga tgaattgaac gctgagtcag caacaacccc
     8941 ggttgcggtc gacggacgat ggggattaac acggcagcct gacgatgatt taggggttcc
     9001 cggttaattt gcgggcgcaa aagttgaaag cgagataaaa aatcatcaag cgtcaggcta
     9061 cggtattcca cgtcttactt ctccagttgg cgtaatatct tattaacttt atcaaaagtt
     9121 tcctgatatt ccgcttcttc ctggctatcg gcgacaattc caccgcccgc agagcagtat
     9181 atttgtccgt taatggcagt cagcgtgcgg atagtaatgc tggtatccat gttgccgcaa
     9241 aagctcaaat agccaatgct gccgcaccag gcattacgtc gctgcggttc cagttcgtcg
     9301 ataatttcca tagcccgtac tttcggagcc ccggttattg agccaccagg aaaagctgcg
     9361 cgcagcagat cgctggcgtg taactgttct ggtagtcgcg ccgttatagt gctgaccaga
     9421 tgatgcacgg cagggaaggg ttccaccacg aagagctctg gtacttttac cgaaccggct
     9481 acggcaacac gaccgatatc attacgcatt aaatcgacaa tcatcagatt ttcggcacga
     9541 tctttcgctg agttcgccag tttttctgct tgtttgctat cttcctgagg atcgggcagg
     9601 cgtggtagcg tgcctttaat cgggcgagtc tggatttcac tattatcaca aagaataaac
     9661 cgctctggcg aaaggcttaa aattgcaccc tgttcaagac gtaaaaaagc gctaaatggc
     9721 gcgcggttgg cctgattaag ctgaaggaat gcctgccatt catcgccaga ataggtcgca
     9781 tgaaaacgct gggcgagatt cacctgatag caatcaccgc tgtgcagata ttcctgtacc
     9841 tggcgaaatt tttcgccgta ctgctcgtgg gtcatattgg attgccagtc ggaagtgagc
     9901 gtgaaatctt cctgcggcga gaattgctgg ctttccagcc aggcccgacg ggcattgaca
     9961 tcattatgac tcagcaaaga aactgtatga cgctggtggt cgacaatgag cgcccaatcg
    10021 tagataccca ctgccatatc cggcagaacg atatcttgtt ccgcaatttc tggcagtgac
    10081 tcaaaacggc ggcccagatc gtagccaaac aaccccagtg cgccgccctg aaatggcaaa
    10141 tcttcgttat gcgttgggcg aatgtctgcg cgatccagca cctgctggag cacctgtagc
    10201 gggtcatcag tggtcgttgt gcgtttttcg ctttcactaa caacggtttc tttacctaaa
    10261 gtggttaaag tgcaaatcgg atcggcgacc acaatatcaa agcggctata cggatgatcg
    10321 gcatagccgg agtgtaaaag catcgcccac ggcaggtggc ttaagcggga gaaataaaat
    10381 tcagcggcgt cctgacgcca gggtaaagta atcacagtgg gagataacgt cttcatcagt
    10441 cctgactcta ctggctatgt gccgggtagc atgaaataat tagcgccaac aatttagcag
    10501 gagttaacaa tgtttgcagg tttaccttca ctcatccatg aacagcagca aaaagctgtc
    10561 gagcggatcc aggaactgat ggcacaaggg atgagtagcg gccaggcaat cgcgctggtg
    10621 gcagaagagc tgcgtgccaa ccactccggc gagctaatcg tggcgcgttt tgaggatgaa
    10681 gacgagtaac acactaatcc gtatcgattt cgttatagaa gccgatgatc atttgattgt
    10741 gagaaaaagg acgcttttta tatgtgatta aaagcgtcct gacccattta tgctgatata
    10801 aatttaactc agaccagtgc ttttagttct tcctgcatat atgttgaaaa gtattcagga
    10861 tttttaatca cagagcgttc acctgtctcc attttttttg cccatccaac cattttttcc
    10921 gtgaacccag ggttccagcc ttcctggtca gctcgcgccg taatatcagc ggcactcgcg
    10981 ggtactccca gttcttttaa atatttaaag attcccttcg ccgtgctttc attcatcgaa
    11041 cttggtgttg gcgctgatac atccatggca ccaatgaaat ccagggcttt atctattaca
    11101 gcaggcatat tcttatcctt aaaattaacc atgttatcga taaggctata tatgacctta
    11161 atttgcatct aatatcaaag ggattataac gatatggcac gagttaacct gttgacattc
    11221 aggtgtttta aaggtagcag ctcatggtgt gttatccagt aagatggaga aaattaacgg
    11281 tcagcccgca cgctataacc agattgttca gctaagaaag aaaaccagag ttgctaatca
    11341 aagggcgaac acaacggcat ggttatgttt atttatatag ttagttttat agataaaatt
    11401 gaaattataa atataaataa tgaattagca gaagcatcaa cagtaatatc ttattctctg
    11461 gtcgatttat gtaaaaccga ccagtattat cgataaatta tcgtctgact tattttgctt
    11521 cgataaagct tacaccgcag ccacaatctt aatttcaact ttatacttcg ggttcatcaa
    11581 acccgcttgt accgtgcagc gcaccggcgc atgacccgcg acaacccagg catcccacgc
    11641 tttattcatc gccgcgaagt cgtttttatc ggccaggaaa atggtggcat ccagaatgct
    11701 cgatttattg ctgccctgtt tttccagcac ggcgtcaatc tgtgccagcg tgttggcggt
    11761 ttgctcaaag gcatcggcgt cgaggttttc cggtacacca gtgtagtaga gcgtgttgtt
    11821 gtggattact acatcagacc agcgggcttc agcatcgata cgaacgatag tcatcatttt
    11881 gtcctcatta tacttccatt ttcgtgctgc aaagactgcc acaatgtgag gttgccgtca
    11941 ccacttcaac tggcgaaagc gccctcatcc tgacataatc cctgttcaaa tcaacagggg
    12001 gtagtgtgac ggacgatttt gcaccagacg gtcagctggc gaaagcgata ccaggcttta
    12061 agccgcgaga accacagcga cagatggcgg tagccgtcac ccaggcgata gaaaaaggcc
    12121 agccgctggt ggtggaagca ggaaccggta cgggcaaaac ctacgcttac ctggctcctg
    12181 cgctgcgggc gaaaaagaaa gtcattatct cgaccggctc aaaagcgttg caggatcagc
    12241 tctacagccg cgatttgcca acagtctcaa aggcattgaa atatacgggc aacgtggcgc
    12301 tgctgaaagg gcgctcaaac tacctctgcc tcgaacgtct cgaacagcag gcgctggcgg
    12361 ggggcgatct gccggtacaa atcttaagcg atgtgatcct gctgcgctcc tggtctaatc
    12421 aaacagtcga tggtgatatc agcacctgcg tcagcgtggc ggaagattca caggcgtggc
    12481 cgctggtcac cagcaccaac gacaactgtc ttggcagcga ctgcccgatg tataaagatt
    12541 gctttgtggt caaagcacgt aaaaaagcga tggacgccga tgtggtggtg gtaaaccatc
    12601 atctctttct ggcggatatg gtggttaaag agagtggatt tggcgagttg atccctgaag
    12661 cggacgtcat gatcttcgac gaagcccacc agctaccgga cattgccagc cagtattttg
    12721 gtcagtcact ctccagtcga caactgctcg acctggcaaa agacatcacc atcgcctacc
    12781 gcaccgaatt aaaagacacc cagcagttac aaaagtgcgc tgatcgtctt gcccagagtg
    12841 cgcaggattt tcgtctgcaa ctcggtgagc caggttatcg cggtaacctg cgtgagctgt
    12901 tagctaatcc gcaaattcag cgggcatttt tactgctcga tgacaccttg gaactttgtt
    12961 atgatgtggc gaaactgtca ctggggcgtt ccgccttgct ggatgcggca tttgagcgcg
    13021 ccacgttgta tcgcacacgg ctgaagcggc taaaagagat caatcagccg ggctacagct
    13081 actggtacga atgcacttcg cgccatttta ctctggctct cacgccgctc agcgtggcgg
    13141 ataaattcaa agagttaatg gcgcaaaagc ccggtagctg gatcttcacc tcagcaacgc
    13201 tgtcggtgaa cgacgatctg catcatttca cctcgcggct tgggatagaa caggcggagt
    13261 cgttgctatt gccaagcccg tttgattaca gccgccaggc gttactctgt gtgccgcgca
    13321 atctgccgca aaccaaccag ccaggttctg ctcgccagtt agcggcaatg ctgcgaccga
    13381 tcatcgaagc taacaacggt cgttgtttta tgctttgtac ctcgcacgcc atgatgcgcg
    13441 atctggccga gcagttccgc gctaccatga cgcttcccgt tttgttgcag ggggaaacca
    13501 gtaaaggcca actgttgcag cagtttgtca gtgccggtaa tgcgcttctt gtggcaacca
    13561 gtagtttctg ggaaggggtg gacgtgcgtg gcgatacatt gtcattggta attatcgaca
    13621 aattgccgtt tacctcgccg gatgatccac tgttaaaagc gcgcatggaa gattgtcgtt
    13681 tgcgcggtgg cgacccgttc gatgaagtgc aactaccaga tgccgtcatt actctcaaac
    13741 agggggtagg tcgactgatt cgcgacgccg acgatcgtgg cgtgctggtg atttgtgact
    13801 atcggctggt gatgcgtcct tacggcgcga cgtttctcgc cagtctgccg cccgcgccac
    13861 gcacccgtga cattgcccgt gcggttcgtt tccttgcgat accatcctcc aggtaatttg
    13921 ttacggagta tggtatgatg cgcgcctttt tattgatcta agcacgaaga cccatgcgaa
    13981 ttctggctat cgataccgcg acagaggcct gctctgtcgc cctgtggaac gacggtactg
    14041 tcaacgctca ttttgagctt tgccctcgtg aacatactca acgaatctta ccgatggtgc
    14101 aggatatcct gaccaccagc ggaacttccc tgactgatat taacgctctg gcttacgggc
    14161 gcggccccgg tagctttacc ggcgtgcgca ttggtattgg catcgcgcaa gggctggcgc
    14221 ttggcgcgga attaccgatg attggcgtct ccacgctaat gacgatggcg caaggggcgt
    14281 ggcgcaaaaa cggcgcaaca cgcgtgctgt cagccattga tgcgcgaatg ggcgaagttt
    14341 actgggccga atatcagcgt gatgaaaacg gtatctggca cggtgaagaa accgaagccg
    14401 tactcaaacc cgaactcgtt catgaacgaa tgcaacagct ttccggcgaa tgggtgacgg
    14461 tagggacggg ctggcaagcc tggccggatc tcggtaaaga gagcgggctg gttttgcgcg
    14521 atggcgaagt gttactcccc gctgcagaag atatgctgcc gattgcttgt cagatgtttg
    14581 ccgagggtaa aacggtggcg gtggaacatg ccgagccggt ttatttgcgt aacaacgtcg
    14641 catggaagaa acttccgggc aaagaatgaa tcttaagagt atgccctgag aaaaaaagga
    14701 gtcgcatcat ggcggttcaa aagaatgtta tcaaaggcat actggcaggt acgtttgcgc
    14761 taatgctgag cggttgtgtc actgtgccgg acgccattaa aggcagcagt accacgccgc
    14821 aacaagattt agttcgggtg atgagtgcgc cgcagctgta cgttggtcag gaggcacgct
    14881 ttggtggcaa agtggttgcg gtacaaaacc agcaagggaa aacccgcctg gaaattgcta
    14941 ccgtaccgct ggacagcgga gctaggccga cgctgggaga accttctcgc ggtcgcattt
    15001 atgccgatgt gaacggtttt ctggacccgg tggatttccg tggacaactg gttacggtag
    15061 tcgggccaat caccggtgcg gttgacggca aaatcggcaa tacgccctat aaatttatgg
    15121 tgatgcaagt aacgggttac aaacgttggc atttaaccca gcaggtgatt atgccgcctc
    15181 agccgattga tccatggttt tatggcggtc gtggctggcc ctatggctac ggcggatggg
    15241 gctggtataa tcccggcccc gcgagagtac aaacagttgt aaccgaataa ttgcttgttt
    15301 ttaaagaatg cagaaacagc ggctggtccg ctgtttctgc attcttacgg taaagataaa
    15361 aataaatagt gacgcgcttc gcaacctttt cgttgggtaa ttatcaagct ggtatgatga
    15421 gttaatatta tgttaacggc gtgtatatca tttggggttg cgatgacgac gaacacgcat
    15481 tttagaggtg aagaattgaa gaaggtttgg cttaaccgtt atcccgcgga cgttccgacg
    15541 gagatcaacc ctgaccgtta tcaatctctg gtagatatgt ttgagcagtc ggtcgcgcgc
    15601 tacgccgatc agcctgcgtt tgtgaatatg ggggaggtga tgaccttccg caagctggaa
    15661 gaacgcagtc gcgcgtttgc cgcttatttg caacaagggt tggggctgaa gaaaggcgat
    15721 cgcgttgcgt tgatgatgcc taacttattg caatatccgg tggcgctatt tggcattttg
    15781 cgtgccggga taatcgtcgt aaacgttaac ccgttgtata ccccgcgtga gcttgagcat
    15841 cagcttaacg atagcggcgc atcggcgatt gttatcgtgt ctaactttgc ccacactctg
    15901 gaaaaagtgg ttgataaaac cgccgttcag cacgtaattc tgacccgtat gggcgatcag
    15961 ctatctacgg caaaaggcac ggtagtcaat ttcgttgtta aatacatcaa gcgcttggtg
    16021 ccgaaatacc atctgccaga tgccatttca tttcgtagcg cactgcataa cggctaccgg
    16081 atgcagtacg tcaaacccga actggtgccg gaagatttag ccttcctgca atacaccggc
    16141 ggcaccacag gtgtggcgaa aggcgcgatg ctgactcacc gcaatatgct ggcgaacctg
    16201 gaacaggtca acgccaccta tgggccgttg ctgcatccag gtaaagaact ggtagtaacg
    16261 gcactgccgc tgtatcacat ttttgccctg accattaact gcctgctgtt tatcgaactg
    16321 ggtgggcaga acctgcttat cactaacccg cgtgatattc cagggttggt aaaagagtta
    16381 gcgaaatatc cgtttaccgc tatcactggc gttaacacct tgttcaatgc gttgctgaac
    16441 aataaagagt tccagcagct ggatttctcc agtctgcatc tttccgcagg cggtgggatg
    16501 cctgttcagc aagtggtggc agaacgctgg gtgaaactga ccggacagta tctgctggaa
    16561 ggttatggcc tgaccgagtg cgcgccgctg gtgagcgtta acccgtatga tattgattat
    16621 catagcggta gcatcggttt gccggtgcca tcgacggaag ccaaactggt ggatgacgac
    16681 gacaacgaag taccaccggg tcaaccgggt gagctttgtg tcaaaggacc gcaggtgatg
    16741 ctgggttact ggcagcgtcc ggatgctaca gatgagatca tcaaaaatgg ctggttacac
    16801 accggcgaca tcgcggtgat ggatgaagaa ggattcctgc gcattgtcga tcgtaaaaaa
    16861 gacatgattt tggtttccgg ttttaacgtt tatcccaacg agattgaaga tgtcgtcatg
    16921 cagcatcctg gcgtacagga agtcgcggct gttggcgtac cttccggctc cagtggtgaa
    16981 gcggtgaaaa tcttcgtagt gaaaaaagat ccatcgctta ccgaagagtc actggtgact
    17041 ttttgccgcc gtcagctcac gggatacaaa gtaccgaagc tggtggagtt tcgtgatgag
    17101 ttaccgaaat ctaacgtcgg aaaaattttg cgacgagaat tacgtgacga agcgcgcggc
    17161 aaagtggaca ataaagcctg agcgttaagt cagtcgtcag acgccggtta atccggcgtt
    17221 ttttttgacg cccactaaag agaaaacaat ttgaattacc aaatgattac cacggacgat
    17281 gcgctggctt ctttgtgtga agccgtccgt gtctttccgg cgatagccct ggatactgaa
    17341 tttgttcgta cgcgcactta ttacccgcag ctggggttga ttcaactttt cgatggcgag
    17401 catctggcgc taattgatcc tctcgggatc accgactggt caccgctgaa agcgatcctg
    17461 cgcgatccgt ccatcacaaa atttctccat gcaggcagtg aagatctgga agtgttcctc
    17521 aatgtctttg gcgaattacc acaacccttg attgacacgc aaatccttgc tgccttctgc
    17581 ggacgcccga tgtcatgggg tttcgcttcc atggtggaag agtattccgg cgttacgctg
    17641 gacaagagtg aatcgcgcac cgactggctg gccagaccgc tgaccgaacg tcagtgtgaa
    17701 tacgcagcgg cggatgtctg gtatctgtta ccgatcaccg ccaagcttat ggtagaaacg
    17761 gaggcctccg gctggctacc tgcggcgctg gatgaatgcc gcctgatgca aatgcgtcgt
    17821 caggaagtcg ttgcgccgga agatgcctgg cgtgatatca ccaatgcctg gcagttacgc
    17881 acacgccaac tggcctgtct gcaactgtta gccgactggc gactgcgcaa ggcgcgagag
    17941 cgtgatctgg cggtgaactt tgtcgtgcgt gaagagcatt tgtggtcggt cgcgcgttat
    18001 atgccgggaa gtttaggcga actggacagc ctgggtttat ccggtagcga aatccgcttt
    18061 cacggtaaaa cgctgctggc gctggtggaa aaagcgcaga cattgccgga agatgcctta
    18121 ccgcagccga tgcttaacct gatggacatg cctggttatc gtaaagcgtt taaagcgatt
    18181 aagtcgctga ttactgacgt gagcgaaacg cataagatca gcgccgaatt gctggcatcg
    18241 cgtcggcaaa tcaaccaact gctgaactgg cactggaaac tgaaaccgca gaacaatttg
    18301 ccggagctga tttccggctg gcgtggtgag ctgatggcgg aagcattaca caatttattg
    18361 caggaatatc cgcagtaaaa tcttccgaag ccggactggg cgcgctcagc gccacatccg
    18421 gctttggcaa actacaaatc caacaccagg cgtttaccct tcgcacgcga acaacagatc
    18481 aacatacttt gctggctggc acgctcttca tcgctaaaat actgatcccg atggtcagct
    18541 tcaccttcca gtattgccgt ttcgcaggtt ccgcataccc cttcacgaca taaacattcc
    18601 actttcgcgg ctttattatt ttcaataacc tgcaaaatag tcatctcttc cggcaccaca
    18661 aactcttttc cggaacgggc aagcaccagg gtaaatgcat cgccggtttt gtcttcgata
    18721 gcaaattgct caaagtgcag tgtatcggcg gcgatgtcca gacgcgcagc ttcacttctt
    18781 accgcttcaa ttagcgcctc ggggccacag gtataaacgt gtgtgccagg ttcgatatcc
    18841 gccaataatc gcgctaattc cagtcgtgtt ccggttgatg aatgatgcaa atggactttc
    18901 tcagcctgcg gatgctggac tagctcagca cgaaatgcgc aactttctgg atttcgcgag
    18961 cagtaatgta gctgccagtc gatgtcgctg tgttgcaact ctgccatgtg cgacaggaaa
    19021 ggggtgatac caataccgcc cacgataaac agatgctttc tggctgaggg aattagcgca
    19081 aacaggttat taggcgttga gatcgttaac cgatcgccca cttttacctg ctgatgcaaa
    19141 aagcgggaac cgccgcgcga gttttcttcc agtcgaacgg caatctgata actagaggtg
    19201 ttatgcggtg aactcagtag tgaatacgca ttgctgtact ggttatcacc atcgctcatc
    19261 tgcactagga tgtgacttcc tccggtgaac gcaggcagcg gtttgccatc ggttgccacc
    19321 agcgtgaagc gtttcacctg ttcggtaagg ggttcaacct ggctcacctg tacttcaaac
    19381 atttgatagt ctgacattgc tgtactccat caaaaatcag cccagcggcg gaaaataccg
    19441 ccgccgatgt cattagtcct taaacacctg cgccagcaga ttatggaaat gggcgatacc
    19501 atgttcggaa atgccactac cgctactgtc ggccatgatg cgcccctgac cacgatagcc
    19561 acgcgatttc agccctttct gtacgctttc aaccagacgt aaatcttccg gatggaacac
    19621 atcgcgatac cactcaatca gcgatttttg ctcgtcggtt aactcttcat tggtgaagta
    19681 aatatcgtag ttttgcaggg tagtttcaga atccaccggg aattcataaa tgaccgtcat
    19741 catccctttg atcggggtga cgttcagcat cgtgcacggc cacagccaga aaccgtggaa
    19801 tgccgcatcc gtaccctctt caaatttaaa cgactgttcg gacggtttgg cgaaaccgta
    19861 ttgcagcgtc cagttaccgt gcatggtgtg ccagtaacga tcaacctgta cggagtcgga
    19921 gaaacctaga tgcgccggac cacagtgata gcactcgaga tagttatcga caatgttctt
    19981 ccagttggca ggcgtgcggg tggtaaagcg ggccgctagt ttcagattgt ggacttccgg
    20041 gcaggcttcc agcactttcg cgcccagacc ggtgtattga ttcacttgaa gtacgaaaaa
    20101 aaaccgggag gacattggat tattcgggtt ctgatgggat tagatttggt ggggcttgca
    20161 agcctgtagc gcaaatttta gtcgttaatc aatgaaacgc gaaagatagt aaaaaattgc
    20221 ttttgtttca ttgaaaatac gaaaaacaaa aacactgcaa atcatttcaa taacagcttc
    20281 aaaaaacgtt caaaaccgat aacaaccagg ctgtaaccaa atgactcata tcacaaatca
    20341 gctcatgccg tttaggtatg ttacatgtgt gattatgtga ggtgaagtat gttttagctg
    20401 gttcatggtt gttatacggc tttttttaca tcttgtggtt cttgtgaagg agctacaaga
    20461 ccttcctgtt cataaatacc atactttgac aaaatctttt tacgagtatc tgttggtaat
    20521 gattcaagca ttttggctgt ttgtaaggtt tgctgcgaaa taccttgcaa atcagcctgg
    20581 atggaatcat cggaagaaaa aatctcaggc ataagagctt caaatccttt ctttctgaac
    20641 aactcaatcg cagaatgcag ttccttgtcc tctaacaatt cgacaattgc gatcagttta
    20701 gttttcaatg tgctgcggtc aggttgagaa tttttattct cttgattatt tttatgctca
    20761 accattggca tgctctgact ttggataaac cactctattg gacgccctgt tgcgtaggct
    20821 atttgtgcta atcgatctaa agacgggtat gtcttgccag acaggtagtt tctgataacc
    20881 ccatcagaaa gtccgcactt agctgcaaac gcccggcttg attctccatt cattgcctct
    20941 tcaaggcgtt ccttgaaacg cattatttcc gggggcataa aaatgcgctc tgcatttttg
    21001 ctttcttttt cacctgtcat gcccaatctc tctgttatat aaagaatttt acagaattct
    21061 ttgatttgca ctggttaaaa taaaaatgcg catagctttt gaattgagca tttttctgcg
    21121 ctatcgtttt tcccgatagc ggtagccggt atcagttacc gaaaacgtta agagagtaag
    21181 gatcgcagaa tgtcagcaaa aaaccaagac tggcacgcag agcagataaa ggctgctgtt
    21241 cgtatgaagg ggaagacact atctcaatta tccagag
//
LOCUS       DAZSIO010000060        21262 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZSIO010000060 DAZSIO010000000
VERSION     DAZSIO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21262
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.060"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            146..757
                     /gene="ruvA"
                     /locus_tag="ACHX5T_002043"
     CDS             146..757
                     /gene="ruvA"
                     /locus_tag="ACHX5T_002043"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR8467247.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            766..1776
                     /gene="ruvB"
                     /locus_tag="ACHX5T_002044"
     CDS             766..1776
                     /gene="ruvB"
                     /locus_tag="ACHX5T_002044"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR8467248.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1923..2708)
                     /gene="znuB"
                     /locus_tag="ACHX5T_002045"
     CDS             complement(1923..2708)
                     /gene="znuB"
                     /locus_tag="ACHX5T_002045"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR8467249.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2705..3460)
                     /gene="znuC"
                     /locus_tag="ACHX5T_002046"
     CDS             complement(2705..3460)
                     /gene="znuC"
                     /locus_tag="ACHX5T_002046"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR8467250.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3539..4471
                     /gene="znuA"
                     /locus_tag="ACHX5T_002047"
     CDS             3539..4471
                     /gene="znuA"
                     /locus_tag="ACHX5T_002047"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR8467251.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4487..5809
                     /gene="mepM"
                     /locus_tag="ACHX5T_002048"
     CDS             4487..5809
                     /gene="mepM"
                     /locus_tag="ACHX5T_002048"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR8467252.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5929..6900
                     /gene="lpxM"
                     /locus_tag="ACHX5T_002049"
                     /gene_synonym="msbB"
     CDS             5929..6900
                     /gene="lpxM"
                     /locus_tag="ACHX5T_002049"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR8467253.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(7031..8473)
                     /gene="pyk"
                     /locus_tag="ACHX5T_002050"
     CDS             complement(7031..8473)
                     /gene="pyk"
                     /locus_tag="ACHX5T_002050"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR8467254.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(8601..9470)
                     /gene="hexR"
                     /locus_tag="ACHX5T_002051"
                     /gene_synonym="yebK"
     CDS             complement(8601..9470)
                     /gene="hexR"
                     /locus_tag="ACHX5T_002051"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR8467255.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            9807..11282
                     /gene="zwf"
                     /locus_tag="ACHX5T_002052"
     CDS             9807..11282
                     /gene="zwf"
                     /locus_tag="ACHX5T_002052"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR8467256.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            11517..13328
                     /gene="edd"
                     /locus_tag="ACHX5T_002053"
     CDS             11517..13328
                     /gene="edd"
                     /locus_tag="ACHX5T_002053"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR8467257.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            13365..14006
                     /gene="kdgA"
                     /locus_tag="ACHX5T_002054"
                     /gene_synonym="eda"
     CDS             13365..14006
                     /gene="kdgA"
                     /locus_tag="ACHX5T_002054"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR8467258.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(14062..15240)
                     /gene="purT"
                     /locus_tag="ACHX5T_002055"
     CDS             complement(14062..15240)
                     /gene="purT"
                     /locus_tag="ACHX5T_002055"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR8467259.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            15374..15664
                     /gene="yebG"
                     /locus_tag="ACHX5T_002056"
                     /pseudo
     CDS             15374..15664
                     /gene="yebG"
                     /locus_tag="ACHX5T_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            15731..16087
                     /gene="yebF"
                     /locus_tag="ACHX5T_002057"
     CDS             15731..16087
                     /gene="yebF"
                     /locus_tag="ACHX5T_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR8467260.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            16414..17073
                     /locus_tag="ACHX5T_002058"
     CDS             16414..17073
                     /locus_tag="ACHX5T_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR8467261.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            17283..19343
                     /gene="ptrB"
                     /locus_tag="ACHX5T_002059"
     CDS             17283..19343
                     /gene="ptrB"
                     /locus_tag="ACHX5T_002059"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR8467262.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(19340..20002)
                     /gene="exoX"
                     /locus_tag="ACHX5T_002060"
     CDS             complement(19340..20002)
                     /gene="exoX"
                     /locus_tag="ACHX5T_002060"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR8467263.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(20026..20682)
                     /gene="yobB"
                     /locus_tag="ACHX5T_002061"
                     /pseudo
     CDS             complement(20026..20682)
                     /gene="yobB"
                     /locus_tag="ACHX5T_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
     gene            complement(20784..21014)
                     /gene="holE"
                     /locus_tag="ACHX5T_002062"
     CDS             complement(20784..21014)
                     /gene="holE"
                     /locus_tag="ACHX5T_002062"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR8467264.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            21153..>21262
                     /locus_tag="ACHX5T_002063"
     CDS             21153..>21262
                     /locus_tag="ACHX5T_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8467265.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAA"
BASE COUNT         5401 a         5290 c         5493 g         5078 t
ORIGIN      
        1 aatataacct gttgaataaa ttatactgtg ccatttttca gatcatcggg acacctcgca
       61 agttttcttc atccttcgct ggatatctat ccagcatttt tttatcatac agcattatct
      121 ttgattcatt acgcaggagc gtcatgtgat aggcagactc agaggcatca tcattgaaaa
      181 acaacccccg ctggtgttaa ttgaagtggg cggcgtaggc tatgaagtgc atatgccgat
      241 gacctgtttt tatgaactcc ctgaagcggg tcaggaagcg atcgttttca cccactttgt
      301 ggtgcgtgaa gacgcgcaac tgctgtacgg ttttaacaat aaacaagaac gcacgttgtt
      361 caaagagttg atcaaaacca acggcgtcgg cccgaagttg gccctggcga tcctctccgg
      421 aatgtcagcg cagcagttcg ttaatgccgt tgagcgtgaa gaagtggggg cactggtgaa
      481 actgccgggt attggcaaaa aaaccgccga acgcttgatt gttgaaatga aagaccgatt
      541 taaaggtttg catggcgatc tctttacgcc agccgccgac ctggtactca cgtctcctgc
      601 tagcccggcg accgacgatg ctgaacaaga agcagttgcc gcgctggtgg cgctaggcta
      661 taaaccacaa gaagcaagcc gcatggtgag caaaatcgct cgccctgaca ccagcagtga
      721 aacattaatt cgcgaagccc tacgcgccgc gttatgaggt aaaggatgat tgaagcagac
      781 cgtctgattt ctgccggtac cactttgccg gaagatgtag cagatcgcgc cattcgccct
      841 aaattactgg aagagtatgt tggtcagccg caggttcgtt cacagatgga gattttcatc
      901 aaagcagcga aactgcgcgg cgatgccctc gatcatttgt tgatttttgg tcctccgggg
      961 ttgggtaaaa ctacgcttgc caacattgtc gccaatgaaa tgggcgttaa tttacgcacg
     1021 acttctggtc cggtgctgga aaaggcgggc gatttggctg cgatgctcac taaccttgaa
     1081 ccgcatgacg tgctgtttat tgatgagatc caccgtttat cgccagttgt tgaagaagtg
     1141 ctgtacccgg caatggaaga ctaccaactg gatatcatga ttggtgaagg tccggcggca
     1201 cgctccatta aaattgattt gccgccgttt accctgattg gcgcaaccac gcgcgcaggt
     1261 tcgctgacat caccgttgcg cgaccgtttt ggtattgtgc aacgtctgga gttttatcag
     1321 gtgccggatc tgcaatatat cgtcagtcgc agtgcacgct ttatggggct tgagatgagt
     1381 gatgacggtg cgcaggaagt agctcgtcgc gctcgcggta cgccacgtat tgccaaccgt
     1441 ctgctgcgtc gagtgcgtga tttcgccgaa gtgaagcacg atggcaccat ctcggcagat
     1501 atcgctgctc aggcgctgga tatgttgaat gtcgatgctg aaggtttcga ttatatggac
     1561 cgcaaattgt tgctggcggt aatcgataag ttctttggtg ggccggtagg tctggataac
     1621 ctggcggcag ccattggcga agaacgtgaa accattgaag atgtactgga accttatttg
     1681 attcagcaag gctttttgca gcgtacaccg cgcgggcgta tggcgacgac gcgggcgtgg
     1741 aatcactttg gcataacgcc gccagaaatg ccgtaagtcg gattggtcgc tcgcgccgca
     1801 tccgacactg gcagtgtgtc tgatgcgaca ctgttcgcgt cttatcaggc cagccagaga
     1861 tagcgaaccg taggtcggat aaggcgctcg cgccgcatcc gacaaatgtg ttcagcgata
     1921 gattagctgg cctgcttttt catcatactg ataataaata acagtgccgc acatagcacc
     1981 accgacggac ccgccggcgt atcgtaaaac gcggaaaagg ttaaaccgcc agttactgcc
     2041 accatcccca ccaaaacagc gacaccagcc atctgttccg gcgtacgggc aaagcgacgc
     2101 gcagtagctg caggaataat cagcaacgaa gtaataatca acgcaccgac aaatttcatc
     2161 gctacaccaa tcgtcaatgc cgtcaccagc atcaacaaca atttcacttg ctgtaatttc
     2221 acaccatcaa caaacgccag atccgggcta atcgtcatcg acagcaaatt gcgccattgc
     2281 cagaacaaaa tagccaccac gatgaccacg ccaatcgcaa tagagatgag atcttctggc
     2341 gtcactgcca gtaaatcacc gaacaggtaa gccatcaaat caacacgaat gttagacatc
     2401 agactaacga ccactaggcc cagcgacagg gcactgtgcg ccataatccc taataacgtg
     2461 tcgatcgcca gctgtggacg cttctccagc cataccagac cgcccgccag cagcagcgta
     2521 acggcaatca ccgcatagaa tggattcacg tccagcaaca aaccaaacgc gacaccaagt
     2581 aatgaggcat gagccagcgt atcaccgaaa taagacatac gacgccagac tacaaacgaa
     2641 cccagcggac ccgcggcaca ggcgagcatg atcccggcta accaaccggg aaataataat
     2701 tcaatcatga gcgatcattt ccccgacgca aaacaattcg tccctgtaaa tcgtgacgat
     2761 gattatgatg atggcgatag atacccagtt gttcagcacc acgaggacca aacattgaaa
     2821 taaactccgg atgcagggaa acaacttccg gtgtgccgga acaacaaatg tggtgattca
     2881 ggcacagcac ttcatcggtt tttgccatca ccagatgcag atcgtgagaa accattaaaa
     2941 cgccacaatc cagttcacga cgcaactggt caataaggtc atataacgcc acttggccat
     3001 ttacatccac gccttgagtg ggttcatcca gcactaataa ttgcgggcaa tttaacaatg
     3061 ctcgcgctaa tagtacacgc tgcgtttcgc caccagagag cttttgcatc ggtgcgttaa
     3121 tcaggtgccc ggcctggaca cgtttcagtg caggcaaaat atcttcttta tgtgtaccag
     3181 ggcgtaagcg taaaaaacgg tttacggtca gtggcaacgt ggtgtcgaga tacagcttct
     3241 gcggtacata gccgatgcgc agttttccgt tgcgcttgat aaccccttca tcgggtgtta
     3301 ccagcccgag cactacccgt accagtgtcg acttacctgc gccatttggc ccaagtaaag
     3361 tcaaaatttt tccaggttta agttccagcg acacatcaga gaggacgcgg cgttggccaa
     3421 aagaaaccga gacattttcc agggaaacca gacttgtcat gttaatttta gtcttgcagt
     3481 agtcatgaaa tgttataata tcacacttct catattcatt acgatgattg gtcgcattat
     3541 gttacataaa aaaacgcttc ttttcgcagc attatccgcc gctctctggg gaggtgcaac
     3601 acaggccgca gatgccgccg ttgtcgcttc gcttaaaccc gttgggttca tcgcttctgc
     3661 cattgctgat ggtgtaacag aaacggaggt tttactgcct gacggcgctt cagaacatga
     3721 ttattcactg cgtccatcgg atgtaaaacg cttacagaac gcggacttag tcgtttgggt
     3781 tggcccggag atggaagcgt ttatgcaaaa accggtaagc aaattaccag aagcgaagca
     3841 ggtaacgatt gcgcagcttg aagatgtgaa accgctactg atgaaaagta ttcacggcga
     3901 tgatgatgat cacgaccacg cggaaaaaag tgacgaagat caccatcacg gcgatttcaa
     3961 catgcatctt tggctttccc cagagatagc gcgggctaca gcggttgcaa tccatggaaa
     4021 attagtggaa cttatgccgc aaagtcgagc caaacttgac gccaacctga aggattttga
     4081 ggcacaattg gcctcaaccg aaacgcaggt tggtaacgag ctcgcgccgc tcaaggggaa
     4141 aggttatttc gtttttcacg atgcttacgg ctatttcgaa aaacagttcg gactgacacc
     4201 gcttggtcat tttaccgtta accctgagat tcaacctggc gcgcagcgtt tacatgaaat
     4261 aagaacacag ttggttgagc aaaaagcaac ctgcgttttt gctgagccac agttcaggcc
     4321 agcggtcgtt gagagcgtcg cacgagggac atccgttcgt atgggaacgt tggatcccct
     4381 ggggacgaat atcaaactgg gtaaaacaag ctattcagaa ttcctgagtc aattagccaa
     4441 ccagtatgcg agctgcctga aaggagatta atgaggaagt gattacgtgc aacagatagc
     4501 ccgctctgtc gccctggcgt ttaataattt accgcgacca caccgcgtta tgttggggtc
     4561 gctcaccgtt cttactctgg ccgtcgctgt ctggcggccc tatgtttatc accgtgatgc
     4621 cacgccaatt gtcaaaacca ttgagctgga acagaacgaa attcgttcgc tcttacctga
     4681 agccagtgag ccgattgatc aagctgcaca agaagatgaa gccattcccc aggacgaact
     4741 ggatgacaaa atcgccggtg aagcgggcgt gcatgaatat gttgtttcca ctggcgatac
     4801 gctaagcagc attctcaatc agtatggtat tgatatgggt gatatcaccc aacttgctgc
     4861 tgccgacaaa gaattgcgta acctgaaaat cggtcaacaa ctctcctgga cattaaccgc
     4921 ggacggcgaa ctgcagcgcc tcacctggga agtgtctcgt cgtgaaaccc gaacctatga
     4981 ccgtactgcc gctaacggtt ttaaaatgac cagcgaaatg cagcaaggag agtgggttaa
     5041 caatctgctg aaaggtaccg tcggagggag ctttgttgcc agcgccagaa acgccggttt
     5101 aaccagcgcc gaagtgagcg cagtgattaa agccatgcag tggcaaatgg atttccgcaa
     5161 actgaaaaaa ggcgatgaat ttgcggtatt aatgtctcga gaaatgcttg atggtaaacg
     5221 tgagcaaagc cagctgctgg gcgtacgttt gcgttcagaa ggtaaagatt attacgcaat
     5281 ccgcgctgag gatggcaaat tctacgatcg taacggtact ggtctggcga aaggattctt
     5341 gcgattcccg acggcgaaac agttccgtat ctcgtctaac tttaacccgc gtcgaactaa
     5401 tccggtgaca ggtcgcgttg caccacacag aggtgttgat ttcgccatgc cacaaggtac
     5461 gccagtgctt tcagtgggtg acggtgaagt ggtggttgcc aaacgcagtg gcgcagcagg
     5521 ttattatgtg gctattcgtc atggtcgcag ctacaccacg cgttatatgc acttgcgcaa
     5581 gattctggtg aaaccgggac agaaggtgaa acgtggcgac cgtatcgcgc tttccggtaa
     5641 taccggacgt tcaactgggc cgcatctgca ctatgaagta tggataaacc agcaggccgt
     5701 aaacccgctg acggcaaaac tgccgcgtac cgaagggctg accggctccg atcgtcgcga
     5761 attcctggcg caggccaaag agattgtgcc gcagctacgg tttgattaat taacatccat
     5821 tcgcagccgg tacgcagtca gtaccggctt tttttatttg gtgcggggca agttgtgccg
     5881 ctacactatc accagattga tttttgcctt atccgaaact ggaaaagcat ggaaacgaaa
     5941 aaaaataata gcgaatacat tcctgagttt gataaatcct ttcgccaccc gcgctactgg
     6001 ggagcatggc tgggtgtagc agcgatggcg ggtatcgctt taacgccgcc aaagttccgt
     6061 gatcccattc tggcacggct gggacgtttt gccggacgac tgggaaaaag ctcacgccgt
     6121 cgtgcgttaa tcaatctgtc gctctgcttt ccagaacgta gtgaagctga acgcgaagcg
     6181 attgttgatg agatgtttgc caccgcgccg caagcgatgg caatgatggc tgagttggca
     6241 atacgcgggc cggagaaaat tcagccgcgc gttggctggc aagggctgga gatcatcgaa
     6301 gagatgcggc gtaataacga gaaagttatc tttctggtgc cgcacggttg ggccgtcgat
     6361 attcctgcca tgctgatggc ctcgcaaggg cagaaaatgg cagcgatgtt ccataatcag
     6421 ggcaacccgg tttttgatta tgtctggaac acggtgcgtc gtcgctttgg cggtcgtctg
     6481 catgcgagaa atgacggtat taaaccattc atccagtcgg tacgtcaggg gtactgggga
     6541 tattatttac ccgatcagga tcatggccca gagcacagcg aatttgtgga tttctttgcc
     6601 acctataaag cgacgttgcc cgcgattggt cgtttgatga aagtatgccg tgcgcgcgtt
     6661 gtaccgctgt ttccgattta tgatggcaag acgcatcgtc tgacgattca ggtgcgccca
     6721 ccgatggatg atctgttaga ggcggatgat catacgattg agcggcggat gaatgaagaa
     6781 gtcgagattt ttgttggtcc gcgaccagaa caatacacct ggatactaaa attgctgaaa
     6841 actcgcaaac cgggcgaaat ccagccgtat aagcgcaaag atctttatcc catcaaataa
     6901 aaaagcctct cgcgaggaga ggccttcgcc tgatgataag ttcaagtttg cttcagaata
     6961 ttcgaaatct gttgaactat cattgaacta taggccggat gtggcgtttt cgccgcatcc
     7021 ggcaacgtac ttactctacc gttaaaatac gcgtggtatt agtagaaccc acggtactca
     7081 tcacgtcgcc ttgggtgaca atcaccaggt caccagacat caagtaacct ttatcgcgca
     7141 gcagattaac cgcttcgctg gcagctgcta cgccgtcatt agcgctatca aagggcaccg
     7201 gcgtaacgcc acgatagaga gcagtcaggt tcagcgtacg ttcatggcgc gacatggcga
     7261 aaattggtag accagagctg atacgggagg tcatcagcgc ggtacgaccc gattcggtca
     7321 tggtaatgat cgccgtaacg cccttcaggt ggttagctgc gtacattgcc gacatggcaa
     7381 tagcttcttc cacattgtcg aactgaacgt ccagacggtg tttagaaacg ttgatgctcg
     7441 ggattttttc cgcacccagg caaacgcgcg ccatggctgc aacagtttct gacggatact
     7501 gcccagcggc agtttctgca gacagcatca cagcgtcagt accatccaga acggcgtttg
     7561 ctacgtccat gacttctgca cgcgtcggca tcgggttagt aatcattgac tccatcatct
     7621 gggtcgccgt gattaccgct cggtttagct gacgcgcacg acggatcaac gctttctgaa
     7681 tgccgaccag ttccgggtcg ccaatttcca caccgaggtc gccacgtgca accattacca
     7741 cgtcagaggc gaggatgatg tcatccattg catcctggct gcaaacggct tccgcacgtt
     7801 caaccttggc aacaattttc gcatcacatc ctgcatcgcg tgccaggcga cgggcatagt
     7861 tcagatcttc gccacagcgt gggaaggaga cagccaggta atctacgcca atcaacgccg
     7921 cagtcttaat gtctgctttg tctttttcgg tcagcgcttc agccgacaaa ccgccgccaa
     7981 gtttgttgat acctttattg ttggagaggg gaccaccgac ggtgacttcg gtgaacactt
     8041 tcatgccctg aacttccagt acttttaact ggacgcgacc atcgtccagc agcaggatgt
     8101 caccaggcac gacgtcagta ggcaggcctt tgtagtcgat accgactttt tctttgtcgc
     8161 cttcaccttt acccaggttg gcgtcgagca ggaatttatc cccaatattg aggaaaactt
     8221 tgccttcttt aaaggtggat acacggattt tgggcccctg gaggtcaccc agaatagcca
     8281 catgacgccc cagttttgcg gcaatctcac gaactttatc cgcgcgcatt ttgtgatctt
     8341 caggcgagcc gtgagaaaag ttcatacgta caacgttggc acccgccgcg ataacttttt
     8401 caagattatt atcgcgatct gttgctgggc ctaacgtggt aacgattttt gttctgcgaa
     8461 gccttttgga catgtaatac tccgttgact gaaacaacca ggtgttgctt gaacatgaaa
     8521 tccgaatgaa ataacgccgc gatggcgata aattaatctc attcggatgt cgtataagat
     8581 taggacagtg acagtcgctt ttagcgatcg tcacttaaat taagtaactg cttatcaaaa
     8641 cgcgattcct tcagcgcttc cttgacccgc ttcaagttat ctctgaattt tgcaccgcgt
     8701 cgcaaagtaa atcctgtcgc cagcacatct atcacggtca gctgtgcaag tcgagaaacc
     8761 atgggcatat aaatgtcagt atcttccggt acgtcgaggg taattgccag cgttgcttcc
     8821 cgggcgagcg gggtacccgc agaggtgagg gcaatcacca tggcgtcgtt ttcgcgtgcc
     8881 agctgcgcca gctcgaccag attttttgtt cttcctgtgt gagaaatcag caccaccacg
     8941 tctccgtcgc tacaattcat acaactcatg cgttgcagca cgatatcatc ggagtacacc
     9001 accggaacat taaaacgaaa gaacttattc atcgcatcgt gggcaacggc ggcagaagag
     9061 cctaatccga aaaaggcgat tttttttgcc tgagtgagca agtcgacggc gcggttgatg
     9121 gcagatttat ccagtgaatg acggacatga tcgagcgttg ccattgcgga ctcaaatatt
     9181 ttccccgtgt atgattcaac gctgtcatct tcattgacgt tgcgattaac atagggagtg
     9241 ccattcgcca gactttgtgc cagatgaagt ttaaaatcag gaaaaccgcg cgtgtccatg
     9301 ctgcgacaga aacgattcac cgtcggttcg ctaacattgg cttccagcgc cagagcagca
     9361 atactcgaat ggatcgcgtt atcgggcgaa gccagaatga cctcggcaac tttgcgctct
     9421 gatttgctca aatgttccag ctgagactgg attttttcca gcatattcat gatgtaaaga
     9481 gactcacggg taatgacgat ttccgcactg aaagaaatcg aaatgcagtt ttgtcagata
     9541 ttacgcctgt gtgccgtgtt aatgacaaaa gcagataaaa aagttgttat ttttttcata
     9601 acatgatcag tgtcagattt ttaccgaatg gaaaacgatg atttttttat cagttttgcc
     9661 gcgctttgcg cgcttttccc gtaatcgcac gggtggataa gcgtttacag ttttcgcaag
     9721 ctcgtaaaag cagtacagtg caccgtaaga aaattacaag tataccctgg cttaagtacc
     9781 gggttagtta acttaaggag aatgacatgg cggtaacgca aacagcccag gcctgtgacc
     9841 tggtcatttt cggcgcgaaa ggcgaccttg cgcgtcgtaa attgctgcct tccctgtatc
     9901 aactggaaaa agtcggtcag ctcaacccgg acacccggat tatcggcgta gggcgtgctg
     9961 actgggataa agcggcatat accaaagttg tccgcgaggc gctcgaaact ttcatgaaag
    10021 aaaccattga tgaaggttta tgggacaccc tgagtgcacg tctggatttt tgtaatctcg
    10081 atgtcaatga cactgctgca ttcaaccgtc tcggcgcgat gctggatcaa aaaaatcgta
    10141 tcactattaa ctactttgcc atgccgccca gcacttttgg cgcaatttgc aaagggcttg
    10201 gcgaggcaaa actgaatgct aaaccggcac gcgtagtcat ggagaaaccg ctggggacgt
    10261 cgctggcgac ctcgcaggaa atcaatgatc aggttggcga atacttcgag gagtgccagg
    10321 tttaccgtat cgaccactat cttggtaaag aaacggtgct gaacctgttg gcgctgcgtt
    10381 ttgctaactc cctgtttgtg aataactggg acaatcgcac cattgatcat gttgagatta
    10441 ccgtggcaga agaagtgggg atcgaagggc gctggggcta ttttgataaa gccggtcaga
    10501 tgcgtgacat gattcagaac cacctgctac aaattctctg catgattgcg atgtctccgc
    10561 catctgacct gagcgcagac agcatccgcg atgaaaaagt gaaagtactg aagtccctgc
    10621 gccgcatcga ccgctccaac gtacgcgaaa aaaccgtacg cgggcaatat actgcgggct
    10681 tcgcccaggg caaaaaagtg ccgggatatc tggaagaaga gggcgcgaac aaaagcagca
    10741 atacagaaac cttcgtggcg atccgcgtcg acattgataa ctggcgctgg gccggtgtgc
    10801 cattctacct gcgtactggt aaacgtctgc cgaccaaatg ttctgaagtc gtggtctatt
    10861 tcaaaacacc tgaactgaat ctgtttaaag agtcgtggca ggatctgccg cagaataaac
    10921 tgactatccg tctgcaacct gatgaaggcg tggatatcca ggtactgaat aaagttcctg
    10981 gccttgacca caaacataac ctgcaaatca ccaagctgga tctgagctat tcagaaacct
    11041 ttaatcagac gcatctggcg gatgcctatg aacgtttgct gctggaaacc atgcgtggta
    11101 ttcaggcact gtttgtacgt cgcgacgaag tggaagaagc ctggaaatgg gtagactcca
    11161 ttactgaggc gtgggcgatg gacaatgatg cgccgaaacc gtatcaggcc ggaacctggg
    11221 gacccgttgc ctcggtggcg atgattaccc gtgatggtcg ttcctggaat gagtttgagt
    11281 aatatcagcg cttatccttt atgggtattt taccggtaac atgatcttgc acagattgta
    11341 gaacaatttt tacactttca ggcctcgtgc ggattcaccc acgaggcttt ttttattaca
    11401 ctgactgaaa cgtttttgcc ctatgagctc cggttacagg cgtttcactc ataaatcctc
    11461 tgaatgaaac gcgttgtgaa tcatcctgct ctgacaactc aatttcagga gcctttatga
    11521 atccacaatt gttacgcgta acaaatcgaa tcattgaacg ttcgcgcgag actcgctctg
    11581 cttatctcgc ccggatagaa caagcgaaaa cgtcgaccgt tcatcgttcg cagttggcat
    11641 gcggtaacct ggcacacggt ttcgctgcct gccagccaga aaacaaagcc tctttgaaaa
    11701 gcatgttgcg taacaatatc gccatcatca cctcctataa cgacatgctc tccgcgcacc
    11761 agccttatga acactatcca gaaatcattc gtaaagccct gcatgaagcg aatgcggttg
    11821 gtcaggttgc gggcggtgtt ccggcgatgt gtgatggtgt cacccagggg caggatggaa
    11881 tggaattgtc gctgctaagc cgcgaagtga tagcgatgtc tgcggcggtg gggctgtccc
    11941 ataacatgtt tgatggtgct ctgttcctcg gtgtgtgcga caagattgtc ccgggtctga
    12001 cgatggcagc cctgtcgttt ggtcatttgc ctgcggtgtt tgtgccgtct ggaccgatgg
    12061 caagcggttt gccaaataaa gaaaaagtgc gtattcgcca gctttatgcc gaaggtaaag
    12121 tggaccgcat ggccttactg gagtcagaag ccgcgtctta ccatgcgccg ggaacatgta
    12181 ctttctacgg tactgccaac accaaccaga tggtggtgga atttatgggg atgcagttgc
    12241 caggctcttc ttttgttcat ccggattctc cgctgcgcga tgctttgacc gctgccgctg
    12301 cgcgtcaggt tacacgcatg accggtaatg gtaatgaatg gatgccgatc ggtaagatga
    12361 tcgatgagaa agtggtggtg aacggtatcg ttgcactgct ggcgaccggt ggttccacta
    12421 accacaccat gcacctggtg gcgatggcgc gcgcggccgg tattcagatt aactgggatg
    12481 acttctctga cctttctgat gttgtaccgc tgatggcacg tctctacccg aacggtccgg
    12541 ccgatattaa ccacttccag gcggcaggtg gcgtaccggt tctggtgcgt gaactgctca
    12601 aagcaggcct gctgcatgaa gatgtcaata cggtggcagg ctttggtctg tctcgttata
    12661 cccttgaacc atggctgaat aatggtgaac tggactggcg ggaaggggcg gaaaaatcac
    12721 tcgacagcaa tgtgatcgct tccttcgaac aacctttctc tcatcatggt gggacaaaag
    12781 tgttaagcgg taacctgggc cgtgcggtta tgaaaacctc tgccgtgccg gttgagaacc
    12841 aggtgattga agcgccagcg gttgtttttg aaagccagca tgacgttatg ccggcctttg
    12901 aagcgggttt gctggaccgc gattgtgtcg ttgttgtccg tcatcagggg ccaaaagcga
    12961 acggaatgcc agaattacat aaactcatgc cgccacttgg tgtattattg gaccggtgtt
    13021 tcaaaattgc gttagttacc gatggacgac tctccggcgc ttcaggtaaa gtgccgtcag
    13081 ctatccacgt aacaccagaa gcctacgatg gcgggctgct ggcaaaagtg cgcgacgggg
    13141 acatcattcg tgtgaatgga cagacaggcg aactgacact gctggtagac gaagcggaac
    13201 tggctgctcg cgaaccgcac attcctgacc tgagcgcgtc acgcgtggga acaggacgtg
    13261 aattattcag cgccttgcgt gaaaaactgt ccggtgccga acagggcgca acctgtatca
    13321 ctttttaaga cgacaaattt gtaatcaggc gagagaaaac tctgatgaaa aactggaaaa
    13381 caagtgcaga atcaatcctg accaccggcc cggttgtacc ggttatcgtg gtaaaaaaac
    13441 tggaacacgc ggtgccgatg gcaaaagcgt tggttgctgg tggggtgcgc gttctggaag
    13501 tgactctgcg taccgagtgt gcagttgacg ctatccgtgc tatcgccaaa gaagtgcctg
    13561 aagcgattgt gggtgccggt acggtgctga atccacagca gctggcagaa gtcactgaag
    13621 cgggtgcaca gttcgcaatt agcccgggtc tgaccgagcc gctgctgaaa gctgctaccg
    13681 aagggactat tcctctgatt ccggggatca gcactgtttc cgaactgatg ctgggtatgg
    13741 actacggttt gaaagagttc aaattcttcc cggctgaagc taacggcggc gtgaaagccc
    13801 tgcaggcgat cgcgggtccg ttctcccagg tccgtttctg cccgacgggt ggtatttctc
    13861 cggctaacta ccgtgactac ctggcactga aaagcgtgct gtgcatcggt ggttcctggc
    13921 tggttccggc agatgcgctg gaagcgggcg attacgaccg cattactaag ctggcgcgtg
    13981 aagctgtaga aggcgctaag ctgtaaaagt caaaatgccc gatctaggat cgggcatttt
    14041 tgtagcgttt ttttgccggg tttaaccctg tacttttacc tgtccggcgg cgtgcttcgc
    14101 gcgttcaatg gcgtcaacaa cactctctga agtagccagt accaccccca gatgacggct
    14161 gccatcaatt tccggcttac caaataaacg aatctgcaaa tctgcgccta cggcattctg
    14221 cacattatca aacgtgacat tctgactggt cagttgtggc agaataacgg cagaagctgc
    14281 aggaccatac tgacggatcc cgccaacagg aagtccgagg aaggcacgta catgcagggc
    14341 aaactctgag agatcttgag aaattaacgt caccatcccg gtatcatgtg gacgagggga
    14401 gacctcactg aaaatcacct catcaccaca gacaaacagc tcgacaccaa acagaccata
    14461 accgcccagt gccagcacca ctttacgggc aatctcctgc gcacgttcaa gggcaagcgg
    14521 gctcatttgc tgtggttgcc aggattcacg gtagtcgcca tcttcctggc gatgacctac
    14581 tggtgcacag aaatggacgc catccaccgc gctgacggtt agcagggtaa tttcgaagtc
    14641 aaacttaacg acgccttcaa caattacgcg gcccgctccg gcgcgaccgc cttgctgagc
    14701 gtattcccat gcctgagcaa gttgctctgc agagcgaata aacgtctgcc ccttgccgga
    14761 agagctcatc accggtttta caatgcaggg atagccaatg gcggcaaccg cctcgcggaa
    14821 aaggcttttg ctatcggcaa aacgataagt ggaagtgggc agctgcagct cttctgccgc
    14881 caggcgacgg ataccctcgc gattcatcgt taatttcgtt gcgcgagcgc aggggacaac
    14941 attcagtcct tcctcttcaa gttggatcag catatcggtg gcaatagctt cgatctccgg
    15001 cacgatataa tgtggttttt ccagttcaac cacacggcgt aatgcatcac catcaagcat
    15061 attaatgaca tgggagcgat gcgcgacatg catggctggt gcgtcggcat agcgatcgac
    15121 ggcaatcacc tctacgccga gacgctgaca ctcgattgcc acttctttac ccagttcacc
    15181 ggagcctaat aacatcacgc gagttgctgc cggacgcagc gcagtgccta ataacgtcat
    15241 aacgaatatc ccctgattaa ttccgcgcgc agtataaacg aaaacgtttg cgtgtgtctt
    15301 tattgctgat gttgatttct caaccgaaaa gaaatatact gtataaaatc tcagttatta
    15361 tgagaggctg caaatggcgg ttgaagttaa atacgtagtc attcgtgagg gcgaagagaa
    15421 aatgtcgttt accagcaaaa aggaagccga tgcttatgac aaaatgcttg atacggcgga
    15481 tctgcttgac acctagctga caaattctcc agtgcaaatg gaagacgagc aacgtgaagc
    15541 cctttcgcta tggctggcag aacaaaaaga ggtgctgagc accattctga aaaccggcaa
    15601 attaccgtcc ccacaggtcg tcggtgctga atccgaagag gaagatgcct cacacgcggc
    15661 ctgaagcaac ttgcgctcct gacaatttgt accatgcttt ctcctgaatc attgtgctat
    15721 ggagaaaaac atgaaaaaaa gaggggcgtt tttagggcgg ttgttggttt ctgcctgcgc
    15781 atcagttttc gctgccaata atgaaaccag caagtcggtc actttcccaa agtgtgaagg
    15841 tctggatgct gccggaattg ccgcgagcgt aaaacgtgat tatcaacaaa atcgcgtggc
    15901 gcgctgggca gatgatcaaa aaattgtcgg tcaggccgat cccgtggcct gggtcagttt
    15961 gcaggacatt cagggtaaag atgataaatg gtcagtaccg ctaaccgtgc gtggtaaaag
    16021 tgccgatatt cattaccagg tctgcgtgga ctgcaaagcg ggaatggcgg aatatcagcg
    16081 gcgttaatgg cttctgataa ttcgattgac cgttggcgga tgcggcgcga gtgccttgtc
    16141 cgtcccagaa cgcaccacaa tcgtaggcct gagaagacgc aacagcgtcg catcgggcaa
    16201 tcagcatcaa acaatgccac tcaaaacgac aaactacttt cacctaaaat acccttatca
    16261 cctgatatac agtaaatacc ttacctggcg cgaatttctt tgaataacgg cctaccgccc
    16321 cgcttatttg cgcatctttt gtctcgatat acattttctg acatcctcat ccgctatgct
    16381 acaggcacta aaaatggtta ttgaggagca aatatggcta actggttaaa tcaactgcaa
    16441 tccctgcttg ggcaaagcag ttcttctacc tcctcgtctg cggatcaggg attgggcaaa
    16501 ctgttagtgt caggcgcatt aggcggactg gctgggctgc tggtcgcaaa taaatcagca
    16561 cgtaaacttc ttacgaaata tggcaccaac gcgttactgg ttggcggcgg agcggtagcg
    16621 ggtacggtgc tgtggaataa atacaaagat aaaattcgcg cggcgcatca ggacgaaccg
    16681 cagtttggcg cgcaaagtac gccgctggat gagcgtacgg aacgtttgat ccttgcgctg
    16741 gtctttgccg ctaaaagtga tggtcatatt gatgccaatg aacgtgcggc aatcgaccag
    16801 caattgcgtg aagccggcgt ggaagagcag gggcgtgtac tcattgagca ggcaatcgaa
    16861 caaccgctgg atccacaacg cctggctacc ggggtccgca atgaagaaga ggcgctggaa
    16921 atctatttcc tgagctgcgc ggctattgat attgaccatt ttatggaacg cagttacctg
    16981 aatgcactgg gtgacgcgct gaaaatcccc caggatgttc gtgaaggcat tgaacgcgat
    17041 ctcgaacagc aaaaacgcac tttggcggaa taattccgct ctctggatag cctgaagttg
    17101 actatttcgg gctatccaga accgcacagc catttgaaca ggctgatttt ctctgtctgc
    17161 ataacattcg atatccgcgc cattacctgt catcatctaa gcaatgacga ccctgtttcg
    17221 cttgcatccc cggtgagttt tgccaccctt ataagatgtt tccaaccaga aagaacaata
    17281 acatgctacc aaaagccgcc cgcattcccc acgccatgac gcttcatggc gatacgcgca
    17341 tcgataatta ctactggctg cgggacgata cgcgttctca gccaaaagtc ctggactacc
    17401 tgcaacaaga aaatagttac ggtcatcggg tgatggcctc acaacaagcc ttgcaggatc
    17461 gcatcttaaa ggaaatcatc gaccgcattc cgcaaagaga agtttctgcg ccctacataa
    17521 aaaatggcta ccgctatcgg catatttatg aaccaggctg tgaatatgct atctaccagc
    17581 gtcaatcggc attcagcgaa gagtgggatg agtgggaaac attgctcgat gccaacaagc
    17641 gcgcagctca tagtgagttt tattcgatgg gcggaatggc gattacgccc gataacacca
    17701 ttatggcgct ggcagaagat tttctttccc gacgccagta cggcattcgt tttcgtaatc
    17761 tggaaacagg taactggtac ccggaactgc tggataacgt tgaacccagc tttgtctggg
    17821 caaatgactc ctggactttc tactatgttc gcaagcatcc ggtgacgctg ctgccttatc
    17881 aggtctggcg tcacgccatc ggtacgccag catcgcaaga tacactgatc tacgaagaaa
    17941 aagacgatac ctattatgtc agcctgcata aaacgacgtc gaagcactat gtcgtcattc
    18001 atctggccag cgccaccacc agtgaagttc gcctgctgga cgcggaaatg gccgatgccg
    18061 agccgtttgt ttttctgccg cgccgcaaag atcacgaata cagccttgat cactaccagc
    18121 atcgttttta tctgcgttcc aatcgccacg gcaaaaactt tggcttatac cgtacccgta
    18181 tgcgtgatga gcaacagtgg gaagagttaa ttccgccacg cgaaaacatc atgctggaag
    18241 ggtttacgct gtttaccgac tggctggtgg ttgaagagcg tcagcgcggg ttaaccagtt
    18301 tgcgccaaat taaccgcaag acccgggaag tcattggcat tgcctttgat gatccggcct
    18361 atgtgacctg gattgcctac aatccagaac ctgaaaccgc gcgattgcgt tatggttatt
    18421 cttccatgac cacaccagac actttgtttg aactggatat ggataccggt gagcgtcgtg
    18481 tattaaaaca aacagaagtg cctggttttg atgcggcgaa ttaccgcagt gaacacctgt
    18541 ggatagtcgc ccgtgatggc gtcgaagttc cggtttcgct ggtctatcat cgcaaacatt
    18601 ttcgcaaagg acacaacccg ttgctggtgt atggctatgg ttcttacggc gcaagtattg
    18661 atgccgattt cagttttagc cgcttgagtt tgttagatcg tggctttgtc tacgccattg
    18721 tccatgttcg cggcggtggt gagctggggc aacaatggta cgaagacggt aaatttctga
    18781 agaagaaaaa tacgtttaat gattatcttg atgcctgcga tgcattgtta aaactgggct
    18841 atggctctcc ttcgctctgt tatgcgatgg gcggaagtgc ggggggcatg ttgatgggcg
    18901 ttgcaattaa tcaacgcccg gaattattcc acggcgttat cgcccaggta ccgtttgttg
    18961 atgttgtaac aacgatgctt gatgaatcaa ttcctcttac cactggtgag tttgaagagt
    19021 ggggaaatcc gcaggatccg caatattacg agtacatgaa aagctacagc ccgtatgaca
    19081 acgtcaccgc acaggcttat ccgcatttac tggtaacgac cggtttacac gattctcagg
    19141 tgcaatattg ggaaccggca aaatgggtcg ctaaattgcg cgagctgaaa accgatgacc
    19201 atcttttatt gctctgtacc gacatggact caggccatgg cggtaaatct ggccgcttta
    19261 aatcgtacga aggcgtagcg atggaatatg cttttctggt cgcgctggcg cagggaacat
    19321 tacccgctac gcctgcggat taagtatttt ccagataatg tttcagtgtt aaacgcagct
    19381 ccgggctcat gctgtccagg ttattaaata accagcgcag atagcccgga tagcgttcgg
    19441 caacgtcgga aaccgctttg ccacggtatt tgccaaaggt gaaggttgtc atcaacgacg
    19501 gacgtccggt gatatcggcc atctgttctg ccgtccagcc ggaggtgttc ataatatcga
    19561 taagcaacgc ggcggtgata taacagtcat acaacgcgcg gtgatgatgc aggcccagcg
    19621 gtgtctgtac attgagcttg cgtgttttat ataacgccat attgctgtac ttgagccctg
    19681 gccacaaacg acgggccagt ttcatagtgc aaatccactc accgggcatc tcaggcagta
    19741 cgcggcggtc aaagctggcg ttgtgcgcga cataccattc actaccgtaa tagtgtggga
    19801 tcacatcttc aatccacggt ttatcggcga ccatggcttc ggtgatgcga tgaatcgcca
    19861 tcgcttgtgg actaatagga cgatcggggc gcaccaggag gctcatgggg ttgacgattt
    19921 ttccgtcaat gacatcaaca gaggcaatct caacgatccc tccctgcaaa ccgcaggttt
    19981 ctgtatcgat aatgcgcaac atggcatgat ccaggccgaa aaagcctagc gtaatggaat
    20041 gatatcgcct tgccaaccct gtgatgaacg ctgaccgact aacaataatg aaccacgatc
    20101 ggcgcgaacg atgagacgac catattcatc ccatagtgca ctgttaccac gggcatttgc
    20161 catcagcacg gcaatcgagt actgatgtga aaaaaattgt aagcgacggg ccgacgccag
    20221 caggtcgggt tctcccagac attgccctgt cgtaaacagt gaacaggtcg ggtccatatc
    20281 cataccttgc ggttgttcat cgaccaccgt gatggttctg gaacgacggc ccagacatgc
    20341 accatgactc tgatggtagt tcccaggcgt ttttcgccac ggtgcgaaca ccgcaattcc
    20401 acgaataaag cgatcgttat attcaacggg aaggccggca atgatggtca ttcgctaggt
    20461 cgttgcggca taacaaagcg ggtctaacag tgaaagatcg ggtggggcag gaagggcacg
    20521 tcgcgaataa tcacacccca gtaaagaaag cgatggaaaa actaacagct gacattgttg
    20581 cctggcggcg gccctcacaa actccagatg atgagctacc tgttcggtga gtgaggtttt
    20641 acgaggctca tattgcgctg ctgcaacttt ccagaacgac ataatgactt ccttttcaaa
    20701 atgactgcaa attatggggg cggtttagaa aatactcata atttagcagg cgttatgtaa
    20761 gaaagtgtaa ctctgtataa gttttattta agtttgggct cgtaaggtaa acgtgacaga
    20821 ttgaccgaag ccaaacggtg ggcaataagc cgctcgcgaa accagctgcg caaatgttct
    20881 ggctgttcac gttcaaccgc ttcagcgatc accggcatat tgtagcgttc tttaaaagcc
    20941 accccggccg ccgccaaatc gacattcact ttatccattt ctgtttgatc cagtttagcc
    21001 agattcttca gcattcttaa tctcctcatc attcgcgggc aaaagttact aagagtgagc
    21061 ctggatggca agccggaatt gatgatcctc gactcagaca ttagacaagg ttatgctctg
    21121 taaaaaagtt attacaaaaa ggaatagata atatggcttc aactgcacgc tcccttcgct
    21181 acgcgctggc aatcctgacc acttcgttgg tgacaccttc cgtctgggca catgcgcatt
    21241 taacgcatca gtatcctgcg gc
//
LOCUS       DAZSIO010000061        20780 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZSIO010000061 DAZSIO010000000
VERSION     DAZSIO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.061"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..579
                     /locus_tag="ACHX5T_002064"
     CDS             <1..579
                     /locus_tag="ACHX5T_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR8467266.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="ACHX5T_002065"
     CDS             597..1235
                     /locus_tag="ACHX5T_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR8467267.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="ACHX5T_002066"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="ACHX5T_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR8467268.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACHX5T_002067"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACHX5T_002067"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR8467269.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="ACHX5T_002068"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="ACHX5T_002068"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR8467270.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACHX5T_002069"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACHX5T_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR8467271.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="ACHX5T_002070"
     CDS             complement(5552..6613)
                     /locus_tag="ACHX5T_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR8467272.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="ACHX5T_002071"
     CDS             complement(6610..8007)
                     /locus_tag="ACHX5T_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR8467273.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="ACHX5T_002072"
     CDS             8163..9161
                     /locus_tag="ACHX5T_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR8467274.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACHX5T_002073"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACHX5T_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="ACHX5T_002074"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="ACHX5T_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="ACHX5T_002075"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="ACHX5T_002075"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR8467275.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="ACHX5T_002076"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="ACHX5T_002076"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR8467276.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..15286)
                     /locus_tag="ACHX5T_002077"
                     /pseudo
     CDS             complement(14232..15286)
                     /locus_tag="ACHX5T_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(15296..16071)
                     /locus_tag="ACHX5T_002078"
                     /pseudo
     CDS             complement(15296..16071)
                     /locus_tag="ACHX5T_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
     gene            complement(16089..17141)
                     /locus_tag="ACHX5T_002079"
     CDS             complement(16089..17141)
                     /locus_tag="ACHX5T_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR8467277.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17172..18014)
                     /locus_tag="ACHX5T_002080"
     CDS             complement(17172..18014)
                     /locus_tag="ACHX5T_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR8467278.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18001..18882)
                     /locus_tag="ACHX5T_002081"
     CDS             complement(18001..18882)
                     /locus_tag="ACHX5T_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR8467279.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18903..20186)
                     /locus_tag="ACHX5T_002082"
                     /pseudo
     CDS             complement(18903..20186)
                     /locus_tag="ACHX5T_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(20200..>20780)
                     /gene="ycjM"
                     /locus_tag="ACHX5T_002083"
     CDS             complement(20200..>20780)
                     /gene="ycjM"
                     /locus_tag="ACHX5T_002083"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFR8467280.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5071 a         5669 c         5052 g         4988 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc
    15721 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    15781 acgctgccgg aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa
    15841 gccattaaga cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc
    15901 ggtggtcact ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac
    15961 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct
    16021 ctcgcggata ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt
    16081 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    16141 tgcatgtagc tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    16201 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    16261 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
    16321 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag
    16381 gagatggtgc cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg
    16441 ctggtttcga taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct
    16501 acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    16561 cgatgggcga taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg
    16621 atggcaattt gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg
    16681 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc
    16741 caggagctgc cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc
    16801 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg
    16861 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    16921 atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct
    16981 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    17041 ccgaaacgca cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    17101 agcgcagcaa cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga
    17161 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    17221 aaaagcgcat acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt
    17281 ccccagatgt aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg
    17341 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    17401 tagacgaaca ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt
    17461 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg
    17521 aagtaacttt tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc
    17581 agcgccatct cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc
    17641 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct
    17701 ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg
    17761 gaggaaacca ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc
    17821 gggttaaaaa tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc
    17881 agcgatatcg cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac
    17941 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt
    18001 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    18061 gatgaagagc accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt
    18121 tttgtagaga tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa
    18181 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc
    18241 gggcagcgtg acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc
    18301 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    18361 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca
    18421 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    18481 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    18541 gaggatcacc agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc
    18601 cacggccagc ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat
    18661 ccacagcgag tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac
    18721 aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    18781 caccatcggc cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag
    18841 cgcaaacggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    18901 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    18961 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc
    19021 acatcgccca tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc
    19081 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    19141 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    19201 acccagtcgg caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg
    19261 gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
    19321 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg
    19381 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
    19441 atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc
    19501 tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg cgttaaagac gttgccctag
    19561 ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc
    19621 gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt
    19681 tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg
    19741 ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca
    19801 atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc
    19861 gctttgcgat cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg
    19921 atcacttccg gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct
    19981 tcttccacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc
    20041 aatttgctga taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat
    20101 acatgggttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat
    20161 aacagcacga tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg
    20221 ccaggggcgc agcgttattt cattacccaa tatatcaact tcactaatca gatcccgacc
    20281 atgcagatca gtaatattta catgctgaat atttttactg acattaaaca atccagtcag
    20341 acaattacca tcagcgttac ttctttgaat acgcattacg gatgaattaa ccgtatcgat
    20401 ggtaaaatta ttatccggat gaaactcgtt atggctgcga cgaagtgtaa ttaaacgcga
    20461 caactcatga tataccgcat gccttaatgt agcttcatcg ttcagttctc gtgttatctc
    20521 ttcactgtga tattttttac ggttaatcgc acggttatat ccgagttttt cgacacctgc
    20581 gtaatcatta cgcgagccaa gaatactttg aatatatatc gcgggtacac cggggaaact
    20641 taacaaaatc gcatgggcaa ggataaacct ggtgcaacgt tcttcatcgc tactctcgcg
    20701 gcggcttaac gcattcatat aggtgacatt catctcatag ggactgcgcg tgccgtcggg
    20761 attatttttc cagtttacta
//
LOCUS       DAZSIO010000062        20713 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZSIO010000062 DAZSIO010000000
VERSION     DAZSIO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.062"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(77..4537)
                     /gene="mukB"
                     /locus_tag="ACHX5T_002084"
     CDS             complement(77..4537)
                     /gene="mukB"
                     /locus_tag="ACHX5T_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR8467281.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4537..5241)
                     /gene="mukE"
                     /locus_tag="ACHX5T_002085"
     CDS             complement(4537..5241)
                     /gene="mukE"
                     /locus_tag="ACHX5T_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR8467282.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5222..6544)
                     /gene="mukF"
                     /locus_tag="ACHX5T_002086"
     CDS             complement(5222..6544)
                     /gene="mukF"
                     /locus_tag="ACHX5T_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR8467283.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6541..7326)
                     /gene="cmoM"
                     /locus_tag="ACHX5T_002087"
     CDS             complement(6541..7326)
                     /gene="cmoM"
                     /locus_tag="ACHX5T_002087"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR8467284.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7462..8241
                     /gene="elyC"
                     /locus_tag="ACHX5T_002088"
     CDS             7462..8241
                     /gene="elyC"
                     /locus_tag="ACHX5T_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR8467285.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8218..9111)
                     /locus_tag="ACHX5T_002089"
     CDS             complement(8218..9111)
                     /locus_tag="ACHX5T_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR8467286.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="ACHX5T_002090"
     CDS             complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="ACHX5T_002090"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR8467287.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="ACHX5T_002091"
     CDS             complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="ACHX5T_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR8467288.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10242..11474)
                     /gene="ycaQ"
                     /locus_tag="ACHX5T_002092"
     CDS             complement(10242..11474)
                     /gene="ycaQ"
                     /locus_tag="ACHX5T_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR8467289.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="ACHX5T_002093"
     CDS             complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="ACHX5T_002093"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR8467290.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="ACHX5T_002094"
     CDS             complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="ACHX5T_002094"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR8467291.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14279..16538)
                     /locus_tag="ACHX5T_002095"
                     /pseudo
     CDS             complement(14279..16538)
                     /locus_tag="ACHX5T_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="ACHX5T_002096"
     CDS             complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="ACHX5T_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR8467292.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="ACHX5T_002097"
     CDS             complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="ACHX5T_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR8467293.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="ACHX5T_002098"
     CDS             complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="ACHX5T_002098"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR8467294.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19829..20593)
                     /gene="ycaL"
                     /locus_tag="ACHX5T_002099"
     CDS             complement(19829..20593)
                     /gene="ycaL"
                     /locus_tag="ACHX5T_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR8467295.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4981 a         5728 c         5031 g         4973 t
ORIGIN      
        1 ccgttatgtg ccattttttt agtgcagaaa cggagttttc ggaaaaagaa aaggcggcat
       61 tgctgccgcc ttaattttaa ctcgcctggg aaggcgcttc gtctcttcct agaagcgttt
      121 cagggagttg cggcgcaaat cctcgcaggc cgacgacgtg aacatgttcg gtattctgga
      181 agactttacg taccagttta taggtggtgc ctttttccgg actgatattt tccggcgctg
      241 cgatgatgag ctgcatttgc aaacgttcac acaattcaaa cagcgtggcg atagaacgag
      301 catccagtcg cgctgcttca tcgaggaaca gcaggcggca aggagagata tctttaccgc
      361 gcaggcggcg agattcatct tcccagcttt gtaccaccat caccagaatc gacataccgg
      421 taccaatcgc ctcaccggtc gacaatgcac cagactctgc gcgcaaccaa ccatcggaac
      481 cacggttaac ctcaacttcc atttccagat agttgcggta atccagcagt tcttcgccaa
      541 tggtctgcgg cgtgcgctgc cccatatcaa tctgcggatt aagacgctga tacaatttcg
      601 ccaacgcttc cgagaaggtc agacggttgc tgttaaacag atcctgatgc tgctcgtgct
      661 gttcagagag cacatccagc agcatggcgt gcgtttcacg cacgttcacg ttgagacgca
      721 cgctgttcac ctgaccaaac gatacgttct gcaacccctg gttgagcata cggatacggt
      781 tctgctcgcg ctgaatggtt ttgcgaatga tgttcgccac gctgcgggaa ctgatcgcca
      841 gtttctgttc acgggaggtt aattcttcgg tcagacggct aagttcaatc tccatctgtt
      901 cgatagcttc caccggatca tcggtacgaa taatatcctg acgaatacgt tcacgcagat
      961 gctgataaac cgccacgaag aactgaattt tacgctccgg acgtttcgga tcttccgaca
     1021 tgcgcagcac gtcgcgcaga tgttcgttat ccgccaccgc cagacgcagc gcacctaacg
     1081 ccttatccga catggaacgc aaatcatcag cggagagata agccagctca cgacggtgta
     1141 agcggcgctc aacgccgtta tctttcacca tgcgcatcac cgcacaccag cccgctttgg
     1201 cggtcactac ctgctcgcgc atctcaaagt aatcccgctc cagcttgcgc agtttgcggg
     1261 tcaggttgtc catctccgct tcgcagaagg taagcgcttt ttccagttga ttgcggcgtg
     1321 aacggttatt gctcagttgc gcatgcagct cgtcacggcg aatacgcgcc cgctcttctg
     1381 ccccgctatc agcacgcacg ccgatatcct gcaattcacg ttgcagatcg ttgagtagct
     1441 cttttttggt gtcgtaagaa cttttcagcg aagccagcac ctggttgtac tgactcaact
     1501 gcgctgcgtg accgcgcaac gcttcgcgag cgcgggtacg ctccgcttcc gcctgttcca
     1561 gacgttcacg cagtttttcg ttgagatcgc tgttaccgct aagcatttct gccgagtcag
     1621 aatagctaaa gtgcgcacga cgctgcacca cttccgtcag ggcaaacgct tgctgacggg
     1681 catcgcgctg catctgctga gagtacgcgt aatcttcttt taactgttcg aactgttccg
     1741 ggtcgctctg caataccgaa acgatcggtt ccagtttcgc cagttgattg ccaaactgct
     1801 gaacaaaacg cgcggcttcc tgggcttcat ccagacgttc gcggatttca tcgacgcgat
     1861 ccgccaggct gtcatcagcc aacaggttga gacgcggcag aatgcggttc agcgccgtaa
     1921 cgccttcttt cgcctgctca aactgaatac gctgctgctg gttatcattt tcatgattac
     1981 tcaacgcccg ctccagttcg actcgacggc tgttcagttg acggatttct gcttccgggt
     2041 cagactcaaa cgccaccgcc agatgactgc cgataaagcg gctgaacgcc tgatgcagac
     2101 gctgggtttt ctgtacatca aaggagagcg tggcgaagcg ttcggaaagc acttcacgct
     2161 cggcatggag gctttcaata cggctttcac gcgcagcacg accaaacagc ggcacttccg
     2221 ggaaacgtga ataacgccac tgtcgatcgg cgattttcac caccaccgct ttttccagct
     2281 catcaacgct gaacacgcta tcatcgaatg actgcggatc cccttcgatc aaatagagat
     2341 cttccgggca atcggtcaag ccttccaggt gttcggttac ctgtgacaga tctggcacca
     2401 cgatggcgtg gcgtgacggg ccatacagag ccgagaagta cggcgcatct tccaggctaa
     2461 cgtcgtcata aatttctgac agcagcacac caccaaaacg ctccgccagc gcgttcagac
     2521 gctgatcttc agagccgcca ggctggctta aacgttcgat ctcttcatcg acggcgtttt
     2581 tgcgcgcgcc tacttcatcg cgttcaacaa tcgcctcgcg ctcacgctcc agcaactgtt
     2641 gcagatattc agtgacgtcc tggctggagg agaactcttc gccgcactgt tcactcaact
     2701 ggttgagact gttttgcgct gccagccaaa ccggtgcacg ctgcatcaaa ctctgaatgc
     2761 gagactgcag ctgttcctgt tcctgccgca gtgccatccg ctcttcacgg gcgttagata
     2821 cgctatcgga aagagaggca atgcgtgctt ccagctcctg atgcagagct tccagttcat
     2881 caatatcaaa attcttgccc tgacgtttgc agaaatctgc cagcagacgc tcagcttctt
     2941 gctgctcacg cagacgctgt tccagttcgc ttaagcgcat ccgcaacggc tgaacctgct
     3001 ctgccaggtg acgctgatca accccttcgc gcaatagttc gcgagcgaca tcccacgcct
     3061 cgttacgcgc cagcgggccg ttgattgcca ccaccagctg ataagcctgc tcaaactggc
     3121 tgtgcgcggt ttgcgccatg ctcattttct gctcaagaga gagcattttt tcagtcgctt
     3181 ccagctcttt cgcctggaag gtttccagcc attcggcggc gctgtcggcg gttaagtccg
     3241 gcagatggca cagttctttg gcacgattaa gcgcagcaat cgcctggtta tactggatcg
     3301 cgcgcgtttg ctggacgtcc agcgcctgct ggtagtcagc aagctggctt ttcagctcgt
     3361 ccacttccag ttcggcagct tccgcgcgag cctcattctc ttcctggcgt tcgatggctt
     3421 ctgccaccac ttcattttgc tcttccagac ggatctgcag ctcatcgaga tccacttcgt
     3481 agcgttcgat cttctcctgc tgacgcaatg cggtttgcac caggttcagg tgatcgctgg
     3541 ccgcctgata atccgcttcc agatcacctt cggcaccgtt gtgctctgcc agctcacgcg
     3601 ccatatcgac gtgtttgtac tgctcagccg ccagttgctg acgcgaagta tgtagctcgc
     3661 gacgaaactc cagggctttg tcgagatgga cacggcgctc gttggcgtga cgcatgtagt
     3721 ccgccgccac gtagttggta gcttcgctga tcagatgctt aaacaggtca cggtccgact
     3781 gggtgacacg aatcgcttcc agcgtcatgc ggttttcacg cagcgccgct tccatgtcct
     3841 ggaacgcttt acgcacgccg ctgttttccg gcaacaggta gtcgcgcaga gaacgggtaa
     3901 tggcactgga gatcccgcca tacagcgaag cttcgatcag acgatagaat ttgctacggt
     3961 cagatgcgga gcgcagacga cgcgcgatga tgcccagatc gaacatcagc gagtggtaat
     4021 cggtaatgga gttgaactgt ttaaactgca cgccttccat cgcctcgagc ttgtctttca
     4081 gctcgttaag cggcagcacg cgcgcctggc gttcgttcag ggtttcggtc accagctgtg
     4141 tcggctgcac cgacatcggc agtccctgaa tggcaaatgg tttgatatcc actttacgat
     4201 cgcgtccggc aacctgttgc agacgcacac cgaccaccac gcgctggtgg cgcgagttaa
     4261 tggtgtcgag catcgaataa cagacacccg ctttcagctt accgtgcaga cctttatcgc
     4321 gcgaaccgct ggtggccccg gcttccgtag tgttacggaa atgcagcagg gtcaggtcgg
     4381 ggatcagcgc cgtaacgaac gccgccatcg tggtggattt acccgccccg ttaccgccgg
     4441 aaagcgtcgt gaccagctcg tcaaggtcaa aagttcgggc aaaaaagccg ttccagttaa
     4501 tcagcgtcag tgagcgaaat ttaccgcgtt caatcattat tcttcctctc cgctatctgg
     4561 ctgactctct tcggtttcat cgttgagttg cagatgattt tcaatcggca ttgcttcgcc
     4621 atcacgaatc aggcgacgct gtgcttcacg gggatcgtcg ccagcacgca catcggctcc
     4681 gaagcggaac accgattcgg taatgcggaa cttgctgctg tcgtggccca taaaccacac
     4741 catgcctaaa cgacgcagac ggttgagcga agaacgtact ttctcctgca acttctgacg
     4801 gtcaacgtct gaaccggttg aacggttgtt caccagtttc agcagtttcg cttcatcggc
     4861 cagggtgagc agttcgtcgt acagttcctg ctgggtgaaa atcccctcat tcgccagccg
     4921 ttccgggctg agatagagat aacagaggat tttcccgacc atcatatcca gttccgacaa
     4981 gacggaacgc gggataagag tggtggaacg tgggcgtaaa tagaagaacc cttccggtgc
     5041 gcgaataagc tcaacgttat aacgcgcgta aaactcttcc agatattcct gaaaatccat
     5101 caggaatgca tgattatcca gttcgtcgag gccaatatgg cggcctgaac gtaaggcgct
     5161 gtccagcgcc ggaaataacg gattcgccag cgcctgcgcc agcttaaccg gcatcacttg
     5221 ttcaatattt gtcgatgaca tgcgcctgta ccttggctcc gtaatcatta atcggctgcc
     5281 atttcgctgg cagtccggtg aaatctgctt gcgctacgcc aagacgtacc gcctgatcaa
     5341 taacaatacg cgcaacgtca aagtgacgtg cacgcggata ctgtgacaga tattcgcgta
     5401 ccaccagacc aagatccagc ggcacttgtc tggttttgta cacggcaagt tgttcttcga
     5461 tgatcgccgc cagctgttcg cggatctcgt taaactcttc gtattccaga tcctcaggaa
     5521 gttccccagt cacttcttca tcgcgcagtg ccatctcttc gtcacgcata tccagcagac
     5581 gatcggcatt ggcataagtt agcgcccacg gctcatcaaa ataggtttgt accgactgac
     5641 gtaaccgctg agcaaagacg cggtttttat ccatatcgat cgcggtacga ataaatttgt
     5701 gtacgtggcg gtcgtagcca atccacaagt cgatggattg ctggccccaa ctgataatac
     5761 gatcgagttt gctctgcaga tcgaacacca gacgatcgac gaaatgcaga tcgtcatggg
     5821 tcatcgtcgc atcctgaatg cgcaacagat tagcctgcaa tttgtcgcct gccgcttcca
     5881 gcgtatcctg caattcacgc agcgttccgg aagtttccga aagcaacaat tcacagctgg
     5941 aaatcgccgc ccgccagtct ttgttcagca actgggcgat atcgtccttc acctgctgct
     6001 gctgttcgtc catcagacgt tgcgtcaggt cgatactgtc gaaaatttct gctaccgaat
     6061 atttcagtgg cgcatagaca ttacggtgcc agtgaaattc atcaccgccc tcttcggcgg
     6121 catctgctgc gcgtttgagc tcacccgcca caatcgacaa ctgcatagaa agacgcagcg
     6181 tagaaaactc gcgctgacgg atgtagtagt cagtaatgcc gatgccgagc ggcgtcagac
     6241 ggtaaattgc gttcccttcc gcctgctcgc tggtaaagcg gttcagcaga cgttgacgca
     6301 ccatgtcgtt gatcgcgtta ttggcgcgca cgccgatggt ttcgctggtt tgctcaaacg
     6361 catcactcac atggcggaat gcatccacca gctcgccttc actcatctca ccatccagac
     6421 gctcgccgtt cagcgtggca accgccagca gaaaagagag tcggtctacc ggcagcgaga
     6481 tggagaagtc attttttctg gcccaggcaa ccagttcggg gactgtctgg gaaaattcac
     6541 tcatacttta tccttgctct gcggtttgcg cgcggtgaca tgaatataac gccctagggt
     6601 aatatacggt tcctgacggc aataacgcgt ttctaattca agtaatgctt catagcagtc
     6661 gcgctgctgg tacttctcgc gcagataatc atgaaacacg cgaacgcctg tcttacccat
     6721 aatttgccaa ccagcttctt ccagccacag ataaacctgc gccgggtcgc gtggataatc
     6781 tggcgaaagc gtccgttttt tctttttcgg cattcccgcc tgcacgtaat caaaattccc
     6841 ggcgaccatg ttatgcatca acaaaccatg cgcattgtag aacattaacg acaacactcc
     6901 gcctggacgc aacactgacc acagggtctg caatacactg cggggatcgg ccacccactc
     6961 gagcaccgca tggaacaata tcagatcaac gggcgtttcc aaatgcgaag caacatcctg
     7021 agcggcgcaa tgtataaatt gcatgttgtc gctcacacct tttgcttctg ccgcctgttt
     7081 tgcgcggtcg atcatctgcg cagaaagatc gcataaaata acctgatgcc cgcgctcggc
     7141 cattttgatt gcggtctgcc cttctccgcc gccagcatcc agcacacgca gtttttgcgg
     7201 gcccatttcc gccagcacgc gatcgagatc ctgccacaga atagcctgtc gaagctgccc
     7261 tttggtggtg ccgtaaatgt tacgggaaaa cttttccgca atatcatcaa aattgcgatc
     7321 ctgcattgct ttctccaccg gttggctaag acgttttctg ataaagacgc tattttgtca
     7381 cagccacggc gagaatgaac ccgtctggtg taatatcgct agcgatccct tgctatatgg
     7441 ttaaaaaagg aaccagaaag gatgcttttt acactgaaaa aagtgattgg caatatgttg
     7501 ctgccacttc cgctgatgtt gttaattatt ggcgctggcc tggcgctgct gtggtttagc
     7561 cgttttcaaa aaaccgggaa gatttttatt agcattgggt ggctggcact cttgttattg
     7621 agtttgcaac cggtagccga ccgcctgctg cgtcccatcg aaagcaccta tccgacatgg
     7681 aataattcgc aaaaagtgga ctatatcgtg gtgctcggag gtggttatac ctggaacccg
     7741 cagtgggcac cgagctctaa tttaatcaat aacagtctgc cacgcctgaa tgagggtatc
     7801 cgtttatggc gcgaaaatcc gggttcaaaa ctgatcttca cgggaggcgt agcaaaaacc
     7861 aatacggtga gtacagcgga agtaggtgcc agagttgcgc aatcgctggg cgtaccgcgc
     7921 gagcaaatta tcaccctgga tttgccaaaa gataccgaag aagaagctgc ggcagtgaaa
     7981 caggcgattg gtgacgcccc tttcctgctg gtgacctccg catcacatct gccgcgggcg
     8041 atgatctttt ttcagcagga agggttaaat ccgctaccag ccccggcgaa tcagttggcc
     8101 atcgactcgc cgcttaatcc gtgggaacgg gcaatccctt ctccggtctg gctgatgcat
     8161 agcgatcgtg ttggctatga aacgcttggc cgcatctggc agtggctgaa aggtccgtca
     8221 ggcgagccac ggcaagagtg attttgatgc cagatcgaag ttgcgccgac taaatcgtcc
     8281 ggtattaacc agttgcgcaa cttcatccca taacacatac aaccaacgcc gccagatgaa
     8341 cgactccgcc actggcgcgc gttgcaggta actccagagc aaatcttccg ccagagaatt
     8401 atccattagt cggaacagtt cgtattctcg cggtgcccaa agcattagtc ccggcccgac
     8461 catcgccagt aactgatcgc tgcgcgaatc tttcagcatg ctgcgtaaac agaagttacc
     8521 gtgaatcaac acacaattgt cgttaaagcc ttcaaataat gccgggagac attcgcgagt
     8581 gcgaaacagg atccgcttat cctgcatcgt cagaccggtg ttattgaact gattgagcgt
     8641 ggtccatagc acttcaacat gttgccggta ccatgagggc cagaaatttt cctgagtatt
     8701 gtcgaccgcg ccgacgcaac cgcgactgtc ctgacggtgc caggccagta aggcttcaac
     8761 gatttggtct ttgagctgtt cccagcgttc tggtgttcgg gctggtgcct ccaccgaaac
     8821 accacgcatc cgctccagta gcaggacatc agggccgggg tgttcttcat gcgtcatcac
     8881 accataaata gtcggcatac ggacagtccc acttcttgcc agcatggtgg ttttccatgc
     8941 cagttgtcgg gcttttccgg gcgtactaaa gcttcttgcc attaacggca ttgggtttcc
     9001 ctggctgtca tacaaagccc acaacgccgt atccgctttt tcattgacgc actcaatacg
     9061 actgagtttt tcgcccagta aatggcttaa ttcggcacgc agctgttcca ttcagattac
     9121 cctcataaaa gctactgctt tcataatgag cgccaatata aaagatgtca cctgaatgag
     9181 atcaattgtt gaaaagaaaa attgaggaaa atcgtaacgg tacgacactc ctcccaaaat
     9241 tggctgaagt gtcgtgaagt gaaattagcg catttcagcg cgaacgcgct caagatcttc
     9301 aggggtatcc acacctgtgc caggaacttc ctgagcaaca gcaacatgga ttttttcgcc
     9361 gtaccacaga acacgaagct gctctaacat ttcgatgtgt tctaacggac ttgcctgcca
     9421 gttgacgtaa cgacggataa agcctgcacg gtagccataa ataccaagat gacgcaggaa
     9481 gttatcgcca acggtttcaa ggccttctgc aaaacgatca cgatcccaag gaatggtggc
     9541 acgtgagaag tacagtgcat acccttcagc gtcgagaacc actttcaccg cattcgggtt
     9601 aaacgcttct tccgcattgt ggattggcac cgccagagtc gccataccca cctgacgctg
     9661 agcgaggtta tcagcaacct gacgaatgat tgtcgcaggg atcatcggtt catcaccctg
     9721 cacattaacg atcaccgtgt cgtcgctgaa tgcgcatttt tcgacaactt ccgccagacg
     9781 ttctgttcct gactgatgat cggcgcgcgt catacatact tcaccgccag cggcttcaac
     9841 ggcgcgggca acatcatcat gatcggttgc cacgatgatg cgctcggcac ctgattcacg
     9901 cgcgcgttca agaacatgaa caatcatggg tttgccgtta atatcaacca atggtttacc
     9961 gggcagacgc gtggacgcgt agcgcgcggg aataatgacc acaaaactca tgatttactc
    10021 tcatcagcag tcagcacgcg ggcttccgtt tccagcaaca ctggaatgcc atcgcggagc
    10081 gggaaagcca ggttatccag tttgcaaata agctcttgtt tttcctggtt ataccaaagt
    10141 tttccgttgc aaaccgggca ggcaatgatt tcaagcaaac gatgatccat agttcctcca
    10201 gatggatcgg gttatgaatg cataaatctt atcataatca tttatgcgac ggggtctatt
    10261 tcccagccag tgcggcaatc agtaaagaga ccttgcgggc agcgtcctaa tgtcacccgc
    10321 gttgcctgct gccagctcgc gaaatcagta atcgcctgac gtaacccttt ttgcagcatt
    10381 gtcgttggtt taatgccttc ctgtaaccac agagagataa cttcaaggat gcctgtctgg
    10441 cgatgcattt tggcatccat tcgcccaact aattgcccac gatgtaataa tggcagaaca
    10501 aaatagccat actggcgttt cggcgctggg gtatagcact ccagccggta gctaaaatca
    10561 aaaagctgct ctgcgcgttt gcgatcccag acaacaggat caaaaggcga aagtaccgcg
    10621 ctgtgcgttg cagtgagctt tccggccagc gcacgctcga gtagcggcag caaatcagca
    10681 tgcagccaaa gattgcccaa tttttcaaca tgcacagcaa tgatttgctg ctgttcagcc
    10741 ctcgcttcgc gccacgccgc cagtgccgga cgtttcagcc gatagtaatc tgccagccac
    10801 tgttcgcgga atattcccag gctacgcgca ctgttatcca gcatgatgat ttctgcttct
    10861 gtttgcgaaa cgagatcgcg ctcatcatcc cagtcaggca tgacacggtg ggttaaatca
    10921 taaacgcgct ggaagttgcg ccgttcaatc accatcacct ttccggcagt aaataaacct
    10981 tccagatgcc gtttatgcgg cttccattcc caccagccgc ttgcaccttt acgaggatgc
    11041 tcaaaatcgg ctgaacgtac cggcccttta tcatgaatat gctgaattaa ctgtgcaatt
    11101 tccgcctcat gttcctgcat ccaggcgtct ttgtatttcc agcccatttt ttcaggtgcc
    11161 agcatgcggt ggcgaataag acgaaagtcg ctacgcggca taaagcaggc ttcatgcgcc
    11221 cagtattcca ttaattcgcc acgcgccaga gactcatcca gccactgggc aggataattt
    11281 cccagacgac tgaaaagcac cagatatgga ctacgggcaa caatattgat ggtatcgatt
    11341 tgcagcaagg acatgcggga gatcgttgcc ggaatatcct ccaacgacgc tcgacggcgg
    11401 ggtttgttta acaggccttg tgcggcaagg tgaagattac gcgcatcagc aagggagagg
    11461 tgcggcagcg acattcatga ctccatcaat cgaacgctgc cgcggcgtaa ctagttgcca
    11521 gaagccagcg aggttagttg cgtaagcagt ttcgctggtt catcacctga aagctgtgcg
    11581 tctacaggca aataccacca attttcttct gcaaaggccc ggcatttcac cgcatctttt
    11641 tcagtcatta ccagcgtttg cccggcgctt accaacgcac tgacatcctc atggttcaaa
    11701 gactgatgat cggccagcgg tacacatttt tccggttgta cgccacacat cttcagcgtg
    11761 gcaaaaaagc gcggcggatg cccaatcccc gccatcgcca ctacatgttc aagctgagca
    11821 acgtcacaac gcgtaccggt acgtaaattc accgcctgac ccggcagcag atgcatgggg
    11881 atttcaccgc tgcgagggac accgccgttg acgattaccg catcaaccga ctttaagcgc
    11941 cccgctcgct cacgcattgg ccccgccggc aaccaccagc cattgccaaa gcgacgcaca
    12001 ccatcaataa cgacaatttc cacatcacgc gccagacggt aatgctgtaa accgtcgtcg
    12061 gttacgatga tctgcacatc agggtgttgc gccagaatgg cttttaccgc atcagaacga
    12121 acgggagaaa ccgcaacagg cgcatcagtg cgttgataaa tcaacacagg ttcatcaccc
    12181 gcctgtgctg tggtggtatc tgccgacaat aacagcggat aagattcagc cttaccacca
    12241 tatccccgcg atacgacccc cacgcgaata ccgcgctgtt gcaactgttc caccagccag
    12301 acaacgaccg gggtttttcc gttgccgcct gcggtgagat taccaaccac gacaaccggt
    12361 acgggggcac gccaggcgcg cttcagtttt agtttatagc aaagacggat cgcgccactc
    12421 accaggccat acaaccagga gagtggcagc aatagccgcc acaaagggga ttcaccagac
    12481 cagatttttt cgatcattgg ccaaactgca ttttgtgaag ttgcgcgtaa acgccgcggt
    12541 gctcaagcaa atcgttatgc gtaccgcgtt ccacaatgac accatcctcg acgaccacga
    12601 tttcgtctgc cttttcaatg gtagacaagc ggtgggcaat caccagagag gtacggtttt
    12661 tctgcaactc atccagtgcc gcctgaatcg cacgttcgga ttcggtatcc agagccgagg
    12721 tagcttcatc cagaatcaga atcgggctat cacgcagcaa ggctcgagcg atagcaatac
    12781 gctgacgctg accgccagag agcagcacgc cgttttcacc aatcactgta tcgagaccgt
    12841 tatccatctt attgatgaag tccatggcgt aggccatccg cgccgcttct tcaatttgct
    12901 cacggctgta ctgttcagtc cgtgcgtaag caatgttgtt agcaaccgta tcgttaaaca
    12961 gatggacatt ctgcgacacc agagcaacct ggttacgtaa cgacgccagg gtatactcgc
    13021 gcagatcgtg accatccatc aggatttcgc cttcatcaat atcgtaaaaa cgcgtgatca
    13081 ggctggcgat ggttgattta cctgaaccag agcgtccaac cagagcaacc gtcttccctg
    13141 ccggaatttt caggttgatg ttacgcaatg cgggtacgtc acgtcccgga taagtaaagg
    13201 tgacattgcg gaattccacg tcgccagtcg cacgctcgat cacgcgctta ccttcgtctt
    13261 tctcctgctc actgtccaga atggtaaaca gcgtctgaca agccgccata ccgcgctgga
    13321 actgggcgtt aacgttggtc agcgatttca gcggacgcat cagtgcaatc attgaagaga
    13381 aaacaacggt aatcgtaccg gcagtcaggt tatccataac acttgggaag ctcgccgcat
    13441 acagaacaaa cgccagcgcc aaagaggcga tcagctgaat gatcggatca gagatggaag
    13501 aggctgaaac cattttcatc ccctgaagac gcattcggtt actgacttta tcgaagcgtt
    13561 tcgtttccac ttcctgacca ccgaaaatca atacttcttt gtggcccttc agcatttgtt
    13621 ctgcactggt ggtcacttgc cccatggtgt tctgcatgtt tttactgatg ttgcgaaaac
    13681 gcttcgatac tacgcgaatc gcaatcgaaa caatcggtgc cagcacaatc aaaatgatcg
    13741 acagttgcca actgtaatag aacatcatga tgaacaggcc gatgatcgac gcaccttcac
    13801 gcacaacagt aatcagtgcg ccggaagaag aagaagcaac ctgttcggaa tcgtaggtga
    13861 tacgtgacaa cagcgtaccc gttgactgtt tgtcaaagaa tgaaactggc attcccatca
    13921 tgtgaccaaa caggcggcga cgcatggtca ttaccacctt tcctgatacc caggagatac
    13981 agtagctgga gacatagctg gtgataccac gtaaaatcat cagtccgatc accaccagcg
    14041 gcatccacac cagcacggag cgatctgttt taccaaagcc atcatcaaga agtggcttaa
    14101 ggagcgataa catgaaggta tcgctggctg cgttgaggat taacgctacg cccgccacga
    14161 tcagacccgc tttgaaaggc gcaatggttg gccacagtcg gcggaatgtc tgccacgtag
    14221 agagatcttt gtcgttatgc attcaaaaaa ccagcatttg ttgaaatagc cgcatattct
    14281 acccgttatc cactggcacg ccaaaccact gatgatacca acgaggtaaa atttgctctc
    14341 tgaggctgct aatccgccag ccttgtgctg aaaaattgac cgtcacttgc ccctgatgtg
    14401 gagtatcaag ccattgatat cctttctgtt gatagcgatg cttaactttg ttagagggca
    14461 atcgccatgc gttatagcgc gatgccgatg cgagtgccac ttttccattc actcgctgaa
    14521 ttaatggcaa cgatgatgag gtattactgc catggtgagg tacctgaagc aatgttgcct
    14581 gcacttgctg ccagtaacgg cttagcatct tttgttcagc tggggcttca atatcaccgg
    14641 ttagaagaat gctattcgtc ccgtcatcaa ccttaaccac acaggaatgg ttatttccct
    14701 tatcgttgct accttgtaaa ggccagtgcg cgctgaaacg caatccttgc cactgccacg
    14761 cttcgccacg cacacagggc tgatgatgtt cccagtttaa cggacttctg atccataaca
    14821 tcggccatat atgcaatatt gaatccagcc ctccccggtg atccagatgt tcatggctca
    14881 gaataacgcc ttccggttca agattatgcc agtggagcca ggggataatc agttgttgcc
    14941 cactatcccc ttcaggccag tcctgtgtca tagagaatcg ctttgccgtt tctggctatc
    15001 accattgcca gcccttgccc gacatcaagc atgtacagct gccactcgtc aggtcgaggt
    15061 ttttgccaca gcggccagca catcagcaag cctccagcca cacacattgc tggcaacgtt
    15121 cgccaggcgt ttaatcgcca taccaccagt aagaaccatg gggaaaatga tagccattgc
    15181 caacattcaa caatgttgat ccacccttcc ggcaaactct ttaaccccca gaaaagtaaa
    15241 gccaaagacc ggtcggcaag aaaccataac ccctgttcca ggattaacgg cccgctaaaa
    15301 tgcacaacca tcgcggcgag gatcaacgga accgtgataa atgtcaccaa gggaattgca
    15361 aatagatttg caataaacga ggtcagacta atgccatgaa atatgacgat ttgcatgggc
    15421 ataagcagga gtgtgattcc cagttgcaga tggatgaggg aaacaactgc acgcaatacc
    15481 ggcggcagtt gccactcagg acagggaaac cactgatacc aaaaaatcag tgccgcgacc
    15541 gcagcggcag agagccataa actttgcgag agaatggcaa caggatccat cagcaaaatt
    15601 gccgccagac aacatatcca tacatcccag ccactccact gtcgcccact taacttaagc
    15661 attccccacg tagcaagcgc caccacggta cgcaatgcag gaggttgcat tcccgtcaac
    15721 caggcataaa aagcagcaca gcagattccg ccaattaatg gtatttgcca gtggatccag
    15781 cgcccaggca gaaaaatttg tccactgcga atgagtccgg cagccagcaa cgccgcaaaa
    15841 gcgatgtgca atcccgatat cgccattaaa tgcgccgttc cagtatcgcg catgatattt
    15901 ttgatttctt tggggacgga taaccgttcc cccataccta aaccaagaat aaccgcattc
    15961 cacggatagg gttgcagcgt tgtttgtagt gacgccagat actgtgcacg caggctacaa
    16021 ttcggttcaa tgacacttgc ctgcagaaaa cggccggtga gcggctgatg ctgggcaatg
    16081 gcataacgct ggctatcaaa gccgccatca ttaagttggc catgaactgc acgaactttg
    16141 agtttcattg accattgttg tccagcacaa accgcttgcg gaagttattc gccatacagc
    16201 acaaggcctg gcgcagggaa tatacgtcga ccttgtagat aagtaatttg accgtaatga
    16261 gtggtcatgc cgtcagttgc agtgatctca acaattgcat cctgcgtcgc gccagttaag
    16321 gtttctcctg cccacaaaat ttgctttgct gataatatgc cccaaacgaa aaacagcaac
    16381 gtcagcgcga tataacggac ggttttaaca gggataaatg ccagtacgca ggcgaagaga
    16441 gtcagaaacg caagggttaa tgtcctaggc aattggggca aaatcagcaa cggaaaaatt
    16501 ccgcaaatta tgcatacgcc gactgtcgtt attttcatgc acccctccct gtttatggaa
    16561 agtgtgcaac tttgttgagc ggttgtctgg tgggaatttt gcgttccccg aggcacattc
    16621 agaagaattt aacgaaaggg tgaaaactgc tcaaaactct gcgaggcagc ttccagttcc
    16681 tgattagaga attgaaaaaa gcacccgaca ggtgcttttc tcgcgttcaa gtttgagtaa
    16741 aaacttaacc gtaaatattg gcgcgatcgc gcagttcttt accaggttta aagtgaggaa
    16801 cgtattttcc ttccagttct actttatcgc cagtcttcgg attacgtccg gtacgtggtg
    16861 cgcggtagtg caaagagaaa ctgccgaaac cgcggatttc aatacgctcg ccctgcgcaa
    16921 gagtcgaggc catatgctcc agcatctctt ttactgcatc ttcaaccgtc ttggcgggaa
    16981 tgtgcgattg ctgggtggca agtctttcta tcaattctga cttggtcatg attcctccgg
    17041 ttccttaaag gcaaattaag cggctgctgt tgctgcattg attaagggcg gccgtagccg
    17101 cccttagtgc ttgattacag gacgaaacct gcaatctgtc aagtaaactc aacaaacttc
    17161 ggaataaaaa tcccgaagag tcagagaatt actcgccttt agctgctttg aaagcttcag
    17221 ccattgcgtt gttggagaag tttgcatctt cctgtttgtt aacagttgcg attgcatctt
    17281 tctcgtcagc ttcgtctttc gcacgaacag acaggctgat tgcgcggttt ttacgatcaa
    17341 cgccggtgaa tttagcttca acttcgtcgc caacgctcag aaccagggta gcgtcttcaa
    17401 cgcggtcacg ggatgcttca gaagcacgca ggtaaccttc aacgccgtca gccagttcta
    17461 cggttgcgcc tttagcgtca actgcagtta ctttaccggt tacgatagcg cctttcttgt
    17521 tcagagcaac ccagttgttg aacggatctt ctgcgagctg tttaacgccc agggagatac
    17581 gttcacgttc tgcgtcaacc tgcagaacaa ctgcagcgat ttcgtcgcct tttttgtatt
    17641 cacgaactgc ttcttcgcct gcaacgttcc aggagatgtc agacaggtga accaggccgt
    17701 cgatgccgcc gtccaggccg atgaagatac cgaagtcagt gatagacttg attttacctt
    17761 caacacggtc gcccttgttg tgggtttccg cgaactgctg ccacgggtta gctttgcact
    17821 gtttcagacc cagggagata cgacgacgtt cttcgtcgat atccagaacc ataacttcca
    17881 ctacatcgcc aacgttaaca actttggacg ggtggatgtt tttgttggtc caatccattt
    17941 cggaaacgtg taccagacct tcaacgcctt cttcgatttc aacgaagcag ccgtagtcag
    18001 tcaggttggt cacgcgacca gtcagtttgg taccttccgg ataacgttta gcgatagcta
    18061 cccacggatc ttcgcccagc tgtttcaggc ccagggatac acgggtacgt tcgcggtcga
    18121 acttcagcac tttaacagtg atttcgtcgc ccacgttgac gatttcgctc ggatgcttaa
    18181 cgcgtttcca ggccatgtca gtgatgtgca gcaggccgtc aacgccgccc agatcaacga
    18241 atgcaccgta gtcagtgagg ttcttaacga tacctttaac ttccatgcct tcctgcaggt
    18301 tttccagcag ctgatcgcgc tctgcgctgt tttcggattc gataacggca cgacgagaaa
    18361 caacaacgtt gttgcgcttc tgatccagct tgattacttt aaattcaagc tctttgcctt
    18421 ccaggtgcag agtgtcacgc accggacgaa cgtctaccag agaacctggc aggaacgcac
    18481 gaataccgtt cagctcaaca gtgaagccgc ccttaacttt gccgttgata acaccggtaa
    18541 cagtttcagc atcttcgtaa gctttttcca gcgtgatcca ggcttcgtga cgtttagctt
    18601 tctcacggga cagcagagtt tcaccgaagc cgtcttctac tgcgtccaga gcaacgtcaa
    18661 cttcgtcacc tacctggatt tccagctcgc cctgggcgtt tttgaactgc tcagccggga
    18721 tggcggactc agatttcaga ccagcgtcaa ccagtactac gtctttgtcg atagcaacaa
    18781 caacgccacg aacgatagaa cccgggcggg tttcgatttc ttttaaggac tcttcaaaga
    18841 gttgagcaaa agattcagtc atgtttaatc ttcaggttta tatttaacgt ccacctggct
    18901 ccatgccgga tggggttgtt taacataccc gctgtcattc cattgcaacg ggggtactgc
    18961 aaattcggtc gcttatgcga gagccaattt ctggcgcgcg tattgtagcg ctttttcaat
    19021 caattgctca atgcttaagg tggtggaatc caacactaaa gcatcggctg ccggaaccag
    19081 tggcgctacc gctcggttac gatcgcggtc gtcgcgttct ttgatctcgg ccaaaaggcg
    19141 ctcgaagtta acactaaagc ccttctcctg caactgtagc atgcggcgat gcgcacgttc
    19201 ttccgaggag gcgtcaagga aaattttcac tggtgcatca gggaatacca ccgttcccat
    19261 gtcgcggcca tcggcaatca gacctggtaa ttcgcgaaac gcgcgttggc gacgcaataa
    19321 tgcttcacga acgcgtggga atgccgcgac ttgtgaagcc gcattcgcca cttcctgagt
    19381 acgaatttcg ccgctgacat cttccccttc gaggatcact tccagattgc cattggtcga
    19441 cacaaaacgt acatccagat gggatgccag cggtaccagc gcatcttccg acgcaacatc
    19501 aacatggtga tgtaatgccg ccaatgccag tacgcgataa attgcacccg agtccagcag
    19561 atgccattgc aacgcttccg ccatagcctt acacaaggtg cctttccctg caccgcttgg
    19621 gccatcaatg gtaataaccg gggcaattgc cgtcatcttt atctccttaa aacaggcgta
    19681 ccgttaacat aaccgcgcgc attatacgcg ccgacgccac aaatcgttaa cgttgtgtgc
    19741 aaaaaccagt tttgtctgta ttcagatggg gatttattgc gcaattatag cgggctggca
    19801 gaagaccagc ccgaaagatg acaatgattt acttaccaga ggcgatacga tcacggatgt
    19861 gttgcgcacg ctctgttgat ggtgggtgag agtcaaacat ggactgggtg cgaccgccat
    19921 ccaggctagc cagtttttca aagctgccaa ccagcccctg ggtgctgatg ccacgtttct
    19981 tcaacagatc aaaagagaaa tcatctgctt cggactcttt atcacgggag tatttagcat
    20041 taatagcgcc ttctgcgata tcacccaatt gtgagcggga aagctgggaa gccacaccgc
    20101 tggtagctga aatggcatcg cgtgcggcaa cgatcgcata agaagctttc atttcagcca
    20161 gcgagtgacc caacgcgacg tggcccagtt catggcccag aacaccttca atttcgttgt
    20221 cattcatcat gtccatcagg ccactgtaga cacgaacaca gccgttcgcc atcgcccatg
    20281 cgttgacgtc gctggtcata taaaccttat agttgaccgg cgtgccatta atattgttac
    20341 ccaatgcttt ggcgattttt gccagacgtt taccgtactt gctggattta cttgcgactt
    20401 gattgccgct gtccatttcg gcacagccct gattggcaat cgcttttgca tcggcatcgc
    20461 ttaatgttgc tgctttatag gcatttaaac cggagctgat agccatattg gtatcaatac
    20521 cgtgggtatt ttgacaccct gtcagtaatg ctgcagaggt cgcaatcgcc agcagtaatt
    20581 tagtattctt catgtgaatc atccgttgat taatcatata taaatattat gtatcaacaa
    20641 cttacattga caagcgacgc taccattaat caaaatgact cacaaggtcc gaaaaaaaac
    20701 gccgacaatg tgc
//
LOCUS       DAZSIO010000063        20703 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZSIO010000063 DAZSIO010000000
VERSION     DAZSIO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.063"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="ACHX5T_002100"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="ACHX5T_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR8467296.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="ACHX5T_002101"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="ACHX5T_002101"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR8467297.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="ACHX5T_002102"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="ACHX5T_002102"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR8467298.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2433..3296
                     /gene="pdxY"
                     /locus_tag="ACHX5T_002103"
     CDS             2433..3296
                     /gene="pdxY"
                     /locus_tag="ACHX5T_002103"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR8467299.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACHX5T_002104"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACHX5T_002104"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR8467300.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACHX5T_002105"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACHX5T_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR8467301.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6165..6800)
                     /gene="nth"
                     /locus_tag="ACHX5T_002106"
     CDS             complement(6165..6800)
                     /gene="nth"
                     /locus_tag="ACHX5T_002106"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR8467302.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6800..7495)
                     /gene="rsxE"
                     /locus_tag="ACHX5T_002107"
     CDS             complement(6800..7495)
                     /gene="rsxE"
                     /locus_tag="ACHX5T_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR8467303.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7499..8119)
                     /gene="rsxG"
                     /locus_tag="ACHX5T_002108"
     CDS             complement(7499..8119)
                     /gene="rsxG"
                     /locus_tag="ACHX5T_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR8467304.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8123..9181)
                     /gene="rsxD"
                     /locus_tag="ACHX5T_002109"
     CDS             complement(8123..9181)
                     /gene="rsxD"
                     /locus_tag="ACHX5T_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR8467305.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9182..11212)
                     /gene="rsxC"
                     /locus_tag="ACHX5T_002110"
     CDS             complement(9182..11212)
                     /gene="rsxC"
                     /locus_tag="ACHX5T_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR8467306.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED"
     gene            complement(11205..11783)
                     /gene="rsxB"
                     /locus_tag="ACHX5T_002111"
     CDS             complement(11205..11783)
                     /gene="rsxB"
                     /locus_tag="ACHX5T_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR8467307.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(11783..12364)
                     /gene="rsxA"
                     /locus_tag="ACHX5T_002112"
     CDS             complement(11783..12364)
                     /gene="rsxA"
                     /locus_tag="ACHX5T_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR8467308.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(12441..12881)
                     /locus_tag="ACHX5T_002113"
     CDS             complement(12441..12881)
                     /locus_tag="ACHX5T_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR8467309.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(12967..13182)
                     /gene="ydgT"
                     /locus_tag="ACHX5T_002114"
     CDS             complement(12967..13182)
                     /gene="ydgT"
                     /locus_tag="ACHX5T_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR8467310.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(13455..13580)
                     /gene="blr"
                     /locus_tag="ACHX5T_002115"
     CDS             complement(13455..13580)
                     /gene="blr"
                     /locus_tag="ACHX5T_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR8467311.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            13823..14863
                     /locus_tag="ACHX5T_002116"
     CDS             13823..14863
                     /locus_tag="ACHX5T_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR8467312.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(14899..15900)
                     /gene="add"
                     /locus_tag="ACHX5T_002117"
     CDS             complement(14899..15900)
                     /gene="add"
                     /locus_tag="ACHX5T_002117"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR8467313.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(16004..17176)
                     /gene="malY"
                     /locus_tag="ACHX5T_002118"
     CDS             complement(16004..17176)
                     /gene="malY"
                     /locus_tag="ACHX5T_002118"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR8467314.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(17186..18778)
                     /gene="malX"
                     /locus_tag="ACHX5T_002119"
     CDS             complement(17186..18778)
                     /gene="malX"
                     /locus_tag="ACHX5T_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR8467315.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            18953..19981
                     /gene="malI"
                     /locus_tag="ACHX5T_002120"
     CDS             18953..19981
                     /gene="malI"
                     /locus_tag="ACHX5T_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR8467316.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            20093..>20703
                     /gene="hdhA"
                     /locus_tag="ACHX5T_002121"
     CDS             20093..>20703
                     /gene="hdhA"
                     /locus_tag="ACHX5T_002121"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR8467317.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
BASE COUNT         5299 a         5207 c         5306 g         4891 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tctttttttt acatcccata
     5581 ttaacggggg tgctttctcg cgttatgtct aaaaaacata cgcgcaattg ttatagtggg
     5641 aggggaaacg gcaggtaatg ttcactatct tgcactttca gacaagtcct ttgtaataat
     5701 ctgttacata taactgaggc ggggaattca accattccgg gaaatgttat ccagcgttaa
     5761 atttttcaaa aaacgtaacg gcagattttt tcactgcatt gagaggggga tgatggcttg
     5821 gcggcgatat gttctgctat ttagcgtttt tcctagtgag atagtccagt ttctgaaaaa
     5881 tagccagtgt aatgttttgc aggtcaatat ttgttgcgtg tgtaaattta aatcatttta
     5941 acctgattct tgcaatgttt ttacattgcc acagcatcgt gatcttgatc acgaatgaat
     6001 agaaaatttc gctattaaaa aaacaattaa cctgcatttt tggttatccg caaacggatt
     6061 acctgatgat aacgctaagc aatggcatca ttgacgttaa aaagatgttt taaaagagga
     6121 taaagaaagg ttatcaatgg ggtaatcggt gttacccctt ttcttcagat gtcaactttc
     6181 tctttgtatt cacaaagatc ttcaataata caagagccac agcggggctt gcgggcaatg
     6241 caggtataac gcccgtgcag gatcaaccaa tggtggcagt cgactttaaa ctctgccgga
     6301 accactttca gtagcttttc ttctacctgt tcgacgtttt tccccggcgc aaattgagta
     6361 cgattacaaa cgcggaaaat gtgcgtgtcg acagcaatag tcggccagcc gaatgcagtg
     6421 tttaatacga cgttggctgt tttacgacct acgccgggca gggcttcaag cgcagcacga
     6481 tcttccggaa cctcgccatt atgttgttcc agtaatatac ggcaggtttt gatgatattt
     6541 tctgctttgc tgttataaag gccaatcgtt ttgatatagg ttttcacccc ttcaacgcct
     6601 agttcaagca tcgctgcagg cgtattcgcc accgggtaga gtttcgccgt cgccttatta
     6661 acactgatat cggtcgcctg agcggaaagc agtacggcaa tcagcaattc aaaaggcgaa
     6721 ctgaaattaa gctcggtggt gggatgagga ttgttctcac gcaggcgagt gaggatctcc
     6781 agacgttttg ctttgttcat cagacattcc ctgtttcacc gtttggcaat gcacgttcgg
     6841 cagctgcttc agcacggcgc tttttcattc tttcatcaat caggtatttt cctgccagca
     6901 tcagtcccaa gccaataaat gcacctggtg gcagcatcgc cagcaggaaa ggggagtcgg
     6961 tgtggaaaat ctccacgcgt aatacttttg cccagctacc taacagcgca tctgcaccgt
     7021 caaacaacgt gccattgccg ataatttcgc gtagtgaacc cagcacgaac atggcgcagg
     7081 ttgcgcccgt accaattgaa aagccgtcca gtgccgaaag cgccggacct tttttggcgg
     7141 cgaaggcttc agcgcggccc acaacgatac agttagtgac aatcagcgga ataaaaatcc
     7201 ctaatgattg atacaggcca aaggcgtagg cgttgatcag catctgtaca gcgctgacca
     7261 ccgaggcgat gatcatcacg taaatgggaa tgcggatctc ggctggcgtc cagtgacgca
     7321 gcgtcgaaat ggtcaggttg gtcagcgtca gtaccagcgt agtcgcaagt cctaaaccca
     7381 gagcgttagt ggcagtggat gtgaccgcca atagcggaca aaggccgagc agttggacca
     7441 gtgcagagtt gtttttccac aacccctgaa caataacgtc tttaatttcg ctcacgcttt
     7501 attctccaca ggcaggaagt tgagaaagtt gtgccggtaa cgtctgagcg tacaatccgg
     7561 cgcgttttac cgcattaacc accgcgcggg gagtaatcgt cgcgccggtg aactggtcga
     7621 aatcaccacc atctttcttc accgcccagt gcgcatcatc cgcaccactg attttttttc
     7681 ccgcaaaatg ggtgagccag tcagaaaggc gcaattcgat tttatcgcca agccctggcg
     7741 tttcgtggtg ctctgtcacg cgcgtgccaa gtaccgtgcc attaaaatcg gctcccacca
     7801 gcagctgaat cgcaccggaa tagccatctg gcgcggttgc ttccagaacg gcggctaccg
     7861 gtttgtcatc ctgtttggcg atgtaaaccc gatgctcacc tttacctaac tctggcgcag
     7921 ttaccagata gcaactctgt gcgagcgcat tgttatagcg ttcggctggc agcacctgat
     7981 caaataacgt cttttgttgc agactggcct gttcagcaat tgtcgttttg gtcatctggt
     8041 tgatggccgc agttaaccct gtagaacccg ctgcaaatag tgccagtgta atgccgtgtt
     8101 ttcggatagt tttcagcatg gtttaccctt tgcgatggcc gtagacgcgc ggacgcgtgt
     8161 agtaatcgat cagaggaacc gtgatgttcg ccagcaggac ggcaaaagcc acgccgtcag
     8221 gatagccgcc gaaactgcgg atcaaccaga ctaataagcc cgccagcgcg ccgaacatca
     8281 gccgaccacg attggtcgta gaagcagtaa ctgggtcagt caaaataaag aatgcgccga
     8341 gcatagtcgc accagacagc agatgaattt gcggtgctgc cagtgtttct ggtgagaaca
     8401 accagcccaa cgttgcgcat aacgccagcg ttactaagaa gctgagggga atatgccagc
     8461 gaatcgcttt ctgccatagc aaccacacgc caccagccag ccaggcgaga tttacccatt
     8521 gccaaccagc gcccgccaga ataccgctgt agatcggata ttgcataatc tgttcaaccg
     8581 aatgacctgc gcggacagag gttttgaatg tatccagcgg tgtcgcctga ctaatgccat
     8641 caatacctaa gcgtagcgta ttcatatcac caccactggc ggtatgaccg ctgaaaataa
     8701 cctgtatggc gtcgataaaa ccagggatgt tgaccgcaat ttcatgtggc ggtaaccagc
     8761 tggtcatctg caccgggaag gagatcagta agaccacata accaatcatt gccggattaa
     8821 acgggttttg ccccagaccg ccatacaact gtttagcgat gatcaccgca aacaccgtac
     8881 ccagtacgac catccaccat ggcgctaggg ggggaatact taccgccagc aataagcctg
     8941 tcagcaatgc tgagttatct ttcaacgttg cggctaccga ctgcttgcgt agtttgagta
     9001 cgagagcttc ggctaacaga gcactgaccg acgccaacag gatctgaacg agagtacccc
     9061 aaccaaaaaa ccgcagttgc gctgcgattc ctggcacggc tgcgagcaac accaacagca
     9121 taatgcgcga tgtctggcgc tggttatggg tataagggga gctagctatt ctgaatacca
     9181 tttagtcctc gtttacaacc ttttgctggg cggctttttt ggcctgaacg cgggcaatag
     9241 ccgcggcaac tgcggctttg cgcggatcga cctgttcttc tggttccgca ttagcctgtt
     9301 gctgttccag cttgcgtgct ttggcgcggg caatagcggc ttcgacggca gctttgcgcg
     9361 gatcaacctg ttcttctggc tccgcattgg cctgttgctg ttccagcttg cgtgctttgg
     9421 cgcgggcaat agcggcttcg acggcggctt tgcgcggatc gacctgttct tctggttccg
     9481 cattagcctg ttgctgttcc agcttgcgcg ctttggagcg ggcgatagct gcttcaacgg
     9541 cagttttacg tggttcagca acggttgctg cgtcgttagt ttgctccagt tctgcctgtt
     9601 tcgctctggc ttgcgcttta cgggcttccc gttctgcaat aattgcactg ttatccgggc
     9661 gttcgcccgc tttaatcaca ataggctgtg tagcctgggc ctgtttctct ttcacccgcg
     9721 ccagagcggc agcaatcgca tctttatctt tggctgcagg ttgaacggct gcgctcttat
     9781 gtcgttcaag gcgagtcgct ttttcgcgct ccagacgagc ctggcgcgct tcgaaacgcg
     9841 ctttggcttc tgcggcgcgc ttttcttcct gacgaatagc cgcaatttca gctttttcct
     9901 gacggaaata ttgcaccaag ggaatattgc tcgggcaaac ccacgcgcaa gccccacatt
     9961 caatgcagtc ggcaatgtta tgcgtggtag ctttatcgtg ttgctgacct ttgctgaacc
    10021 agtacaactg ttgcggcaaa agatccacag ggcaggcgtc agcacaggcg ctacaccgga
    10081 tgcagctttg ttcttcctgt ggttcgccaa gttcattggc agagggagcc aacagacagt
    10141 tggtaatctt tacgaccggg acatccagcc atggcaaggt aaagcccatt agcgggccac
    10201 ccataatcac catttgatcg gcagaggggc agaatccggc atcattcaat aaatgacgca
    10261 ctggcgtccc cagccgtgcc cagacgttgc ccgggcgagc gattgcttcg ccagtcaggg
    10321 ttacaacacg ctcggtaatc ggctcgccat caataacggc acgtttcact gcataagcag
    10381 tgccgacgtt ttgcattaat acgccgatat cggatgaacg cccgccatgt ggaacctgct
    10441 tcccggtcag aatgtaggtt aattgtttag caccgccaga aggatatttg gttggaatca
    10501 cccgcagaga aatatcgtta gagtccgcca gcaccgcgcg cagcatggaa atcgcctgcg
    10561 gtttgttatc ttcaatgccg ataagaattt cgcgtggctg cagaatatgc gcaagaatgc
    10621 gaataccctc tacgacctga gccgcgcaat cctgcatcaa acggtcatcg gcggtaatgt
    10681 acggctcgca ctcagcagcg ttgataatca acgtttcaat cttatctcca ccaccctgca
    10741 atttaacgcc tgtcgggaat ccggcaccgc ccagcccggc aacaccaaac tgatgtatgc
    10801 gctcgattaa ctcttcgcga ctgcgagagc gataatcgac ccagccgtcg cgcgggatcc
    10861 agcagtcttc accatcggca tcaataatca cgcttaattc agctaaagct gaaggatgag
    10921 ccgtagagtg gggcgcaata gccgtaacgg tacccgaggt gggcgcgtga acaggcagca
    10981 ttttgccgcg accacgggta agcggctggc cgcgcaatac tttatcgccg acgctaacgc
    11041 acaactcacc ttcagcgcca atatgctgtt tcagtggaat aacaaaatgc tgcgccagta
    11101 gtacctggcg caggggggta ccgttggtct gggttttcat ctccggtgga tggatgccgc
    11161 cgttgaaatc ccagatttta ttttttctga atgcagagaa taacttaagc atggtgttcc
    11221 acgggaatga tacgcacggg aatggtgttc agatcccatt tccaggagtc aggtgtttct
    11281 gcgaccggtt gcaacgagat gcagtgcgtc gggcacggat caacacataa attgcagccc
    11341 gtacagagat cactcattac cgtatgcatg acacgggtag cgccaacgat ggcgtctacc
    11401 ggacaagcct gaatacattt agtgcagcca atacagttat tttcatcaat aaccgccacc
    11461 atccgcgcag gcgttagctc ttgcgcttcg ccatccagcg gctgcggctc gacattaagc
    11521 aactcggcaa tttttagcat cacagcttcg ccacctgggg cgcaacggtt gattttttca
    11581 ccgttacagc tgatggcttc cgcgtagggg cgacagccgg gataaccgca ctgaccacac
    11641 tggctctgcg gtaagatttc gtcaattttc tcaacgaccg gatcgtcttc caccgcaaaa
    11701 cggcgggagg cataacccag aatggcgcca aacgccaggc ccagcaggct cacggcggca
    11761 acggcaatcc agatagcatt cattacaact tcaccaaacc actaaagccc ataaaggcca
    11821 gagacataag acctgcggta attaacgcaa tggcattacc gcgaaaaggt gccgggacat
    11881 cagccacagc aaggcgttcg cggatggcgg cgaagagcac catcaccagc gagaaaccga
    11941 cagcggcgga aaaaccgtac agcgccgact gcaagaaatt gtgcccgaga ttgatattca
    12001 gcaacgccac gccgagcact gcacagttgg tggtgataag cggcaaaaaa atccccagca
    12061 gacggtaaag caccgggctg gttttgcgca ccaccatctc ggtgaactgc acgaccacag
    12121 caatcaccag aataaatgcc agggtgcgca ggtaaatcag attaagtggg atcaaaatcc
    12181 acgtatcgat aagccaggcg caaatagacg tcagcgtcat cacaaacgtt gttgccagcc
    12241 ccatgcccat cgcggtttcc agttttttgg aaacccccat aaacggacag agaccgagaa
    12301 acttgaccag tacaaagtta ttgaccagta cagttccgac aaacagtaac aggtagtcag
    12361 tcatttttcg gcctgaaata aaaaagccgc ctattatccg ttaatccaga gcaggcgaca
    12421 acaggttaac tgtaaggtta ttacggattc acaaacgtcg cttttacccg cgacgagcgt
    12481 ttgaaatagg ggacgatcaa tgcggttgcc aacaaagtaa acagcaactg gcgaacggct
    12541 atgccaactt ccacgggtga aaaagcgaat gcttttactg ccagcaatac ggaaataagc
    12601 agccagataa tatagtgttt aggaacacaa cgccgacgtt taaagaaagc gatggtcaac
    12661 cacagtgtgt aataccacat ggcgatagcg gtaataaaag aaacgcccca taagatttgt
    12721 gtcgtcagtg cttgtccgcc gagcgtttga aatgtttgcg gagaagataa cgcagcggta
    12781 tacagcaaca acgccagcgt cgtactcaac agagcgacta aaagccaggc caacgggcca
    12841 agcaaccagc ctccaatacg ttgtggcgtt gtggtggtca taggttctcc agaatagcga
    12901 cgcaaacagg caaatctaat tttaggggcg ttattataag gcttttataa ccgatcgcta
    12961 ctttctttat tggacatagt gccagacgga cttcggtacc tggccgaggt caaacaaacg
    13021 cccgccagaa accagctctg cgcgacggtg atcggcagca cgatacatat tgatcagttc
    13081 ctgatcgtcc gtcagggtgt aattaagatg gtcgtagagt ttttccagac tttcgagtga
    13141 actgattttg cgaaatttta ataagtagtc ctgaacagtc ataaaaggtc cataaaataa
    13201 ttaataccaa aggggtaggt attattagtg atgataaaat ttcaccaaaa gtgaacgctt
    13261 gtgttattac aataataaga atgcgctgat ggcaaatatt cagggatgaa aaactgctct
    13321 gaaaagagta acgcgcttta ctatccctga caatcaccaa caacatcgaa caagataata
    13381 aattcctggt ttaatatccg acaagtgaaa acatgcaccc ggacgggcag catgtcgctc
    13441 cacaagtgca gagcttactt gtgttgtacc gaagcactct gttcaggtgg ctgatagttg
    13501 tcaatgtgac tcgccacgcc aagaagaatg actgaaacga caagaacgat ccaacctgtt
    13561 aattcaataa gacgattcat tacagcccac atctctcttg attgatccat taacttcagg
    13621 gggtaaatgt tacttagcaa taatagctcc agcaagattt ttactgaggt ttttgcgata
    13681 tttagctttt gtcgttggaa aattcgctat ttttttgact taagttaaac aacatccctt
    13741 attgctggca ggttattaaa ctgttgagcg tgggtaaagg atagtgtcaa atagccatca
    13801 tacttcaatg agaggcaatg acatgagcga caacatccgt gttgggttga ttgggtatgg
    13861 ttatgcgagc aaaaccttcc atgcgcccct gattgcgggc acgcccgggc tggaactggc
    13921 ggtaatctcc agcagtgatg aaacaaaagt aaaagccgac tggccaacgg ttacggttgt
    13981 ctctgagccg aagcatctgt ttaacgatcc caacatagac ctgattgtca ttcctacacc
    14041 caacgatacc catttcccgt tagccaaagc ggcgcttgag gcgggtaaac atgtggtcgt
    14101 tgataaaccc tttaccgtga cactgtcaca agcgcgagag ctggatgcgc tggcaaaaag
    14161 cctggggcgt gtgctgtctg tattccataa ccgccgttgg gatagcgatt tcttgacgct
    14221 aaaaggtttg ctcgcggaag gcgtgctggg tgaagttgct tactttgagt ctcattttga
    14281 ccgcttccgt ccgcaggtgc gcgatcgttg gcgtgaacag ggcggcccag gcagcggtat
    14341 ctggtacgat ttagcaccgc atcttcttga tcaggccatt acgctatttg gtttaccggt
    14401 cagcatgacg gtagatttgg cacagttacg gcccggagcg cagtcgaccg attatttcca
    14461 cgccatcttg tcctatccac agcggcgagt cattttacac ggtaccatgc tggcagccgc
    14521 tgagtcagca cggtatatcg tgcatggatc ccgaggcagt tatgtgaaat atggcctcga
    14581 tccacaggaa gaacgtctga aaaatggcga gcgtctgccg caggaagact ggggctacga
    14641 tatgcgtgat ggcgtactta cccgcgtgga aggtgaggaa cgtgtcgaag aaacgctgtt
    14701 gacggtgcct gggaattatc cggcttacta tgcggcgatt cgtgatgcgt taaatggcga
    14761 tggtgaaaat ccggttccgg caagccaggc aatccaggta atggagttga ttgagctggg
    14821 catcgaatcc gccaaacatc gcgcgacgtt gtgccttgcg tgaaaaatgg atggtgcaat
    14881 ttgcaccatc catttttgtt acttcgcggc gactttttct cgcagtgcgc gtttttcctc
    14941 tgcgctgagg aaagccattt ccagaccatt aatctgtgcc tggcggattt gctcacggga
    15001 taacccagca gctggcgcgg caacggtata ttcgtgaatg atatccactc cctgtacgcc
    15061 gggatcgtca gtgttaatgc tggcacgaat gccatgctca aggaacgttt tcagcggatg
    15121 tgcagccagc tctgctacgg tgctggtctg aatattggag gtcagacagg attcaatacc
    15181 aatttgttgc tcggcgagaa aatccatcag cgcccgatct tcaatggctt ttacgccatg
    15241 tccaatacgc tccgcaccca gttcacgaat cgcctgccag atgctttccg gcccggcagc
    15301 ttcgcctgca tggacggtaa tatgccagcc cgcatcacgc gcgcggttga agtgagaaag
    15361 gaacagactt cccgggaatc caagttcatc accagctaaa tcaagtgcgg taatctggtc
    15421 acggtgggct aaaaaggcct ccagctcttg ctgacaggcg gcttcgccga aggttcggct
    15481 cataatgccg ataagcttcg cctgcacacc aaaggtgcgg caaccttcac gtacgccatc
    15541 gatcaccgct tcgacaacac ccgctacagg cagcttatgt gccattgcca tgtagcctgg
    15601 tgaaaaacgc agctcgacat agcgcaggcc gttacgggct gcatcttcaa tgttttcaaa
    15661 tgccacgcgg cggcaggcat caagagaggc gagaacttta acgccccagt caagtttggt
    15721 cagaaagctc accagatcgg gttcgttggc aatgacctga acgtggggaa tcagtgtttc
    15781 aagggattgt gcaggaagcg agatattata ctggcggcca agttcaagaa tggtctgggg
    15841 acgaatgttg ccatcaaggt ggcgatggat atcagttaat ggcagggtgg tatcaatcat
    15901 ggtcgcactc tttttctgga tataaaagtg cgcaccatta taaaaacaaa gcagggtaaa
    15961 aatccacgat tgcgcaacga ttttttacgt tgcgcaattg gggttagcga acagcgcgga
    16021 tggcgtttat taatccagcc acaccttttt ccagtttcga acgtgggcag ccggcattga
    16081 gacggacaaa accacgacct tcttcaccat aggtataccc cggcatgatc gcgacttttt
    16141 cttgctcgat aagcgctttt tgcaacgcgt tgtcgtcaat attcaacgga cgtagatcaa
    16201 gccaggccag ataagtggat tgcgggatct gccagttgag ttcaggaaac gcggcgttca
    16261 ttttatctga gatatacgtc aggttatctt tcagatagac gcgtaaggca tccagccacg
    16321 gtgcaccttg ctgataggcg gcgatatggg cagttaacgc cagtaccgaa ggggaagaaa
    16381 gcccatcacg tcctttcagt gccgataaat aggcatcacg gctgctgcta ttttctataa
    16441 tcccgtaagc accggtcagc gcgggaatat tgaaactttt cgatcccgat gtcagcaacg
    16501 cccagtctcc ccgcgccaca ttactccagg gaatatgcgg ctgctcgccc cagaccatat
    16561 ccatatggat ttcatcggaa ataacccgca catcatgacg ctcgcacagg tcagccatga
    16621 tctccagctc atcgcacgtc cacactttcc cggtaggatt ctgtgggtta cacaggagca
    16681 taattttaca ttctggtttc gccaaaacgg cttccagctt gcccatatcg caaaaccagc
    16741 cgtcagcctg cttctctaaa gcaacgggca ttactgtgcg ctggttacct tcaatggcct
    16801 tgtaaaatgc gtcataggcg ggcgtgtgga tcaccacgcc ttcacctgtt tcagaccact
    16861 gacgaatcag ttctgaaacc atatagatga cagaagggcc atacaccacc gtctgcgtat
    16921 caattgcggt gtaatgctgg gtggaaaacc agtgggcaat agccgcgaga aactcatcgt
    16981 ttttccagcg gctgtagcca aatacgccgt gcatcaggcg ctgattcagc gcctcgataa
    17041 tgcagggggc agtggcaaaa tccatgtctg aaatcgtgaa cggtaacagg tcagcagtgc
    17101 cgaaacggtc agcgacataa tcccactgtg tacaccatgt gccatgacga tccacgacct
    17161 ttgaaaaatc gaacatatct tatccttatg cctggacagt atgcatcaga ccggccattt
    17221 catcttttac tgactgaact tgtggcccga taacaacctg caggttatgt tgattaagtt
    17281 gtactacgcc aattgcccga ttgtccttca gtgcctgcac attaacaagc gacatatctt
    17341 ccacagacaa acgcagacgg gtaatgcagt tatcgaggct gacaatattg tcggcaccgc
    17401 ctaatgcttc gaggattgca ggaacgttgt aacctgattt acccggcgca ccggcaacgg
    17461 ctttttcgat tgagctggca acttcgctat cgcgccccgg ggttttcaga ttgaagcggg
    17521 tgatagcgaa acggaagatg acgtagtaaa cgacaaacca gattgccgcc accactggca
    17581 ccatgtacca cttggttgac agaccatgca aaataccgaa caccacgaag tcgatgatat
    17641 tgccgtcggt attaccgatg gtgacgccga gcacagacat gacggtgaag ccgaggccgg
    17701 ttaacagcgc gtggatgaca tacagaactg gcgctacgaa caggaacagg aattccagcg
    17761 gttcggtagt gccgccaacg acgcaggcga tcaggccaga aatcagcaga cctttaattt
    17821 tatggcgatt ttccgggcgc gcgcagtgat acatagctaa cgctgcacct ggtagaccgc
    17881 cgagaaacgc aggcatttta ccttgcgaaa ggaaacgcgt gacgctttca gaaaaaccgt
    17941 gagtggtcgg gcaactcaat tgcgcctgga agatggtcag tgcgccgctg acggtttgac
    18001 cgcagacctc ctgcgtgccg cctgcgtcgg taaagcgaat taatgccacc agaatgtgat
    18061 gcagaccaaa cggcaacagc agacgttcac cggtaccaaa cagcatcggt ccgaaatcac
    18121 ccgcgctgtt tatcatatgg cccaagccgc taatacccat ggcgaaaatc ggccagacta
    18181 atggaatcac caggccgaca aggcccatca ccagcgagga gataattggt acgaagcgcg
    18241 taccgccgaa gaatgccagc gcatccggca ggcggatatt atggaaacgc tcatgcagca
    18301 tccagacgat aataccggcg atcaccgcac cgaggatccc ggtatcgatc gactggatcc
    18361 caagaatgct ctggatgtta ttggctttca gaacggcggc atctgtggtt ggcagaatgc
    18421 ctttattggt caaccagaag tttaccgcga ggttcattac cgcataaccg acgaagccag
    18481 cgaatgccgc tacgccttta ttttcgcgtg ccaggcccag cgggatggcg atacagaaca
    18541 tgacaggcag gaaactaaaa gcaaacgagc caatcttact catccaggta aagatagcct
    18601 gcaacacggg gttgcccagg accgggatca gggttatgac atcatggctg ctaagagaac
    18661 taccaatgcc gagcataatg ccgcagaacg acaataatgc cacgggtaac atgaaggttt
    18721 tgcctaactg ctggaagaac tcccacagcg tgactttcgg tgctgttttc gccgtcataa
    18781 aacgactcct cgtagagaac aatctggatt cagtaaattg cgcgagaaga taaaacgttt
    18841 tatcaaattt tagtgagtca taaatcacat tacgcaacga taatagcggg tataagataa
    18901 ataaaaggta aaacgtttta tctgtcacat aatcagggag taggtcatct gcatggctac
    18961 cgccaaaaaa ataaccattc atgatgttgc gctggctgcg ggcgtgtcgg taagtaccgt
    19021 ttcgctggtg cttaatggca aagggcgaat ctctaccgcc acaggagaac gcgttaacgc
    19081 cgccattgaa gagctgggat ttgtgcgcaa tcgccaggcg tcggcgctgc gcggcgggca
    19141 aagcggcgtc attggtttga tcgtccgtga tttatctgcg ccgttttacg ccgaattgac
    19201 ggccggattg acggaagctc tggaagcaca gggacggatg gtttttttgc ttcacggcgg
    19261 taaagacggc gagcagctgg cacagcggtt ttcactgtta ctgaatcagg gtgtcgatgg
    19321 tgtggtaatt gccggggctg caggaagcag cgatgacctg cggcggatgg cagaagaaaa
    19381 agctatcccg gtgattttcg cttcccgtgc cagttatctt gatgatgttg atacggttcg
    19441 cccggacaac atgcaggctg cacagttgtt gacggagcat ctcattcgca atgggcatca
    19501 gcggatcgcc tggctgggag ggcaaagttc ctcattaacc cgtgcagaac gggtgggggg
    19561 ctattgtgca actctactaa aatttggcct gccgtttcac agcgattggg tgttggagtg
    19621 cacttccagc cagaagcaag ccgcggaagc tatcacggcg cttttacgtc ataacccgac
    19681 catcagtgcc gtggtttgct ataacgaaac tattgcgatg ggggcatggt ttggtttgct
    19741 gaaagcaggg cggcaaagcg gggaaagcgg agtcgatcgt tactttgagc aacaggtttc
    19801 gctggcggca tttaccgatg cgacaccaac cacacttgat gatatacctg ttacctgggc
    19861 cagcacgcca gcgcgggaac ttggtatcac acttgcggat cgcatgatgc aaaaaatcac
    19921 ccatgaagag acgcattcac gcaatcttat tattcccgcc cggctcattg cggcgaaata
    19981 atcctctctt tatctgctat acttggtagt gtcctttcct caaggttaat gataaaaaat
    20041 gtggcacaca ggcggtgcct tttattttta ctgaagttca caggaggttt atgtgtttaa
    20101 ttctgacaac ctgagactcg acggaaaatg cgccatcatc acaggtgcgg gtgcaggtat
    20161 tggtaaagaa atcgccatta cattcgcgac agctggcgca tctgtggtgg tcagtgatat
    20221 taacgccgac gcagctaacc atgttgtaga cgaaattcaa caactggatg gtcaggcatt
    20281 tgcctggcgt tgtgatatta cttccgaaca ggaactctct gcactggcag actttgccgt
    20341 cagtaatctg ggtaaagttg atatcctggt taacaacgcc ggtggcggtg gtcctaaacc
    20401 gtttgatatg ccaatggcag attttcgccg tgcttatgaa ctgaatgtgt tttctttttt
    20461 ccatctgtca caacttgttg cgccagaaat ggaaaaaaat ggaggtggcg ttattctgac
    20521 catcacttct atggcggcag aaaataaaaa tataaacatg acttcctatg catcatctaa
    20581 agctgcggcc agtcatctgg tcagaaatat ggcatttgac ctgggtgaaa aaaatattcg
    20641 ggtgaatagc attgcgccgg gggcaatatt aaccgatgcc ctgaaatccg ttattacacc
    20701 aga
//
LOCUS       DAZSIO010000064        20474 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZSIO010000064 DAZSIO010000000
VERSION     DAZSIO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.064"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(83..1732)
                     /gene="actP"
                     /locus_tag="ACHX5T_002122"
     CDS             complement(83..1732)
                     /gene="actP"
                     /locus_tag="ACHX5T_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR8467318.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1729..2043)
                     /locus_tag="ACHX5T_002123"
     CDS             complement(1729..2043)
                     /locus_tag="ACHX5T_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFR8467319.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2243..4201)
                     /gene="acs"
                     /locus_tag="ACHX5T_002124"
                     /pseudo
     CDS             complement(2243..4201)
                     /gene="acs"
                     /locus_tag="ACHX5T_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            4594..6030
                     /gene="nrfA"
                     /locus_tag="ACHX5T_002125"
     CDS             4594..6030
                     /gene="nrfA"
                     /locus_tag="ACHX5T_002125"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR8467320.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6075..6641
                     /gene="nrfB"
                     /locus_tag="ACHX5T_002126"
     CDS             6075..6641
                     /gene="nrfB"
                     /locus_tag="ACHX5T_002126"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR8467321.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6638..7309
                     /gene="nrfC"
                     /locus_tag="ACHX5T_002127"
     CDS             6638..7309
                     /gene="nrfC"
                     /locus_tag="ACHX5T_002127"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR8467322.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7306..8262
                     /gene="nrfD"
                     /locus_tag="ACHX5T_002128"
     CDS             7306..8262
                     /gene="nrfD"
                     /locus_tag="ACHX5T_002128"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR8467323.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8282..10000
                     /locus_tag="ACHX5T_002129"
     CDS             8282..10000
                     /locus_tag="ACHX5T_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR8467324.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQENSLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            9993..10376
                     /gene="nrfF"
                     /locus_tag="ACHX5T_002130"
     CDS             9993..10376
                     /gene="nrfF"
                     /locus_tag="ACHX5T_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR8467325.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10373..10969
                     /gene="nrfG"
                     /locus_tag="ACHX5T_002131"
     CDS             10373..10969
                     /gene="nrfG"
                     /locus_tag="ACHX5T_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR8467326.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11311..12624
                     /gene="gltP"
                     /locus_tag="ACHX5T_002132"
     CDS             11311..12624
                     /gene="gltP"
                     /locus_tag="ACHX5T_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR8467327.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12702..13391)
                     /gene="yjcO"
                     /locus_tag="ACHX5T_002133"
     CDS             complement(12702..13391)
                     /gene="yjcO"
                     /locus_tag="ACHX5T_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR8467328.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13485..15632)
                     /gene="fdhF"
                     /locus_tag="ACHX5T_002134"
                     /pseudo
     CDS             complement(13485..15632)
                     /gene="fdhF"
                     /locus_tag="ACHX5T_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(15830..17296)
                     /gene="mdtP"
                     /locus_tag="ACHX5T_002135"
     CDS             complement(15830..17296)
                     /gene="mdtP"
                     /locus_tag="ACHX5T_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFR8467329.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17293..19335)
                     /gene="mdtO"
                     /locus_tag="ACHX5T_002136"
     CDS             complement(17293..19335)
                     /gene="mdtO"
                     /locus_tag="ACHX5T_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR8467330.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            complement(19335..20366)
                     /gene="mdtN"
                     /locus_tag="ACHX5T_002137"
     CDS             complement(19335..20366)
                     /gene="mdtN"
                     /locus_tag="ACHX5T_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR8467331.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20385..>20474)
                     /locus_tag="ACHX5T_002138"
     CDS             complement(20385..>20474)
                     /locus_tag="ACHX5T_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8467332.1"
                     /translation="RANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4572 a         5430 c         5629 g         4843 t
ORIGIN      
        1 tttgtttaat attatgggaa aaggtgatgc atgttggaga ggaagcgtat tccccggtca
       61 ggcgaccggg gaaggggaga gattaatgtg cgcggccttg ctcaacgcca aagccggtct
      121 gggagcggat aaactgcgcg cggaacagtt cacgctcgcg cgccccttcc gctgagttat
      181 cggttgccga gaagaaccag atgccgagga atgccacgct gatagagaac agcgccgggt
      241 attcatacgg gaagatggct ttttcgtgac cgaggatctg tacccaaata gtcgggccga
      301 ggatcatcag caccactgcg gtaatcagcc ccagccagcc gcccagcatc gcgccacggg
      361 tagtcagttt cgaccagtac atggaaagca gaatgatcgg gaagttacag ctcgccgcga
      421 tggcaaacgc aaggcctacc ataaaggcga tgttctgatt ctcaaacagc acgccgagga
      481 taatcgcaat cacgccgagg atcagtacgg tgatttttga tacccgcagc tcttcacgtt
      541 cggtcgcgcc ttttttgaag acgttagcgt acaagtcatg cgaaaccgcc gatgcgcccg
      601 ccagcgtcag atccgcaacc accgcgagga tagtggcgaa agcaaccgct gaaataaaac
      661 cgaggaatag gttgccgccc actgcattcg ccaggtgaac ggccgccatg ttgttaccac
      721 caatcagatg gcccgccgcg tctttatatt ccggattcgc accaacaagc atgatcgcgc
      781 cgaagccgat aataaaggtc agaatataga agtagcccat aaatccggtg gcgtagaaca
      841 cgctcttacg tgcttcgcgg gcatcgctga ctgtaaagaa gcgcatcaga atgtgcggca
      901 agcccgccgt accaaacatc agtcccagcc ccagagagag cgcggagatc ggatctttca
      961 ccagtccgcc cggtttcatg atgtcgacac ctttcgggtg taccgccatc gcttcgctga
     1021 acagattgtt gaagctaaag ccgacgtgtt tcatcaccat aaaggccata aagctggcac
     1081 cgaacagcaa cagcacagct ttgataattt gtacccaggt ggtcgccagc atgccgccga
     1141 acaggacgta catcatcatc agcacgccga ccagcaccac cgcaatgtga tagttaaggc
     1201 caaacagcag ctcgatcagt ttacctgcgc ctaccatctg agcgataagg taaagcgcca
     1261 ccactaccag agaaccacag gccgaaagaa tacgaatcgg cccttgtttc agacgataag
     1321 aggccacatc ggcagaggtg tagcgtccca ggttacgcag acgttcggca atcagaaaca
     1381 gaatgattgg ccagcccacc aggaagccca acgagtagat cagcccgtca tagccggagg
     1441 taaacaccag cgcggagatc cccaagaatg aggcggcgga catatagtcc ccggcaatcg
     1501 ccagcccgtt ctggaagcca gtgatattgc cgcctgcggt gtagtagtcg ctacgagaac
     1561 gtacgcgttt tgatgcccag taggtaatgc cgagcgtaaa cacgacgaaa atcaggaaca
     1621 taataatcgc ctgccagttc gttggctggc gctctacggc cccgctaata gcatccgcgg
     1681 cgttagctgc gaaagggagt gtggcggcaa gcgccgtcag aactcttttc atgatgcttg
     1741 tacctcatgc aggacttcat tattaagacg gtcgaattcg ccgttcgccc gccagatgta
     1801 gataccggtg agaacaaagg agatcacaat cactccaaca ccaattggaa taccgcgtgt
     1861 gacgctggtg ttcggattca gcggcgtgcc cagccagccg ggcgcgaagg cgatcagtaa
     1921 aataaagccg atataaactg ccagcataat aatcgacagg atggtggcaa accgttgccg
     1981 tttttcgact aactccctga aatgcgcatt gtcttctatc cgctgataaa tcgtaccatt
     2041 catcacagat tctccagagg taatgtaggg attgttttaa ttcccgctcc cttatgggag
     2101 aaggttaacg ctcgggtaac ccttgccgaa tgtaggccgg ataaggcgtt tacgccgcat
     2161 ccggcaatca atgcctgatg cgacgctgtc gcgtcttatc aggcctacaa accgttaccg
     2221 attcgcatcc ggcaattgtg ggttacgatg gcatcgcgat agcctgcttc tcttcaagca
     2281 gcttctcgac cacgccagga tcggcaagcg tcgaggtatc gcccaggttg ctggtatcgc
     2341 ccgccgcaat tttgcgcaga atacggcgca taattttgcc ggagcgggtt ttaggcaggg
     2401 agtcggtcca gtgcagcacg tctggcgtcg ccagcgggcc aatctcttta cgcacccagt
     2461 tgcggacttc tgcgtacagt tctggtgacg gttcctcccc gtgattaagc gtgacatagg
     2521 cgtagatcgc ctgaccttta atattgtgcg gaatacctac tacggcggct tcggcaatct
     2581 tcggatgcgc caccagcgcc gactctatct ctgccgtccc cagacggtga ccggagacgt
     2641 tcagcacgtc gtccacacgc ccggtgatcc agtaatagcc atcttcatcg cgacgcgcgc
     2701 cgtcgccgct gaaatacata tttttgaagg tggaaaaata ggtctgctca aaacgttcgt
     2761 gatcgccaaa cagcgtacgc gcctgaccag gccaggagtc ggtgatcacc aggctacctt
     2821 cggtagcccc ttccagcggg ttaccttcgt tatcgaccag cgccggttgc acgccgaaga
     2881 acggacgtgt tgccgaaccg gctttcagct cggtagcgcc aggcagcggg gtgatcatga
     2941 aaccgccggt ttcggtctgc caccaggtat cgaccaccgg acatttctcg ttgccgattt
     3001 ttttccagta ccactcccac gcttccgggt taattggctc gcccacggaa ccgagaatgc
     3061 gtagcgacga acggtcggtg ccttcaatcg ctttatcgcc ttccgccatc agcgcgcgga
     3121 tcgccgtggg tgcggtatag agaatattga cctgatgctt gtccaccacc tgcgccatac
     3181 gggcaggcgt cggccagttc ggtacgcctt caaacatcag cgtggttgcg ccgcaggcca
     3241 gcgggccgta cagcaaataa ctgtgtccgg taacccagcc cacatcggcg gtgcaccagt
     3301 agatatcacc cggatgataa tcaaagacat atttaaaggt cagcgccgcg tacaccagat
     3361 aaccgccggt agtgtgcagt acgccttttg gttttccggt agaaccggag gtatagagaa
     3421 taaacagcgg atcttcggcg ttcatctctt ccgcctagtg ctgatcgctg gcttgctcaa
     3481 ccaggtcgtg ccaccacagg tcgcgccctt cctgccagtc gatgtttccg ccagtacgct
     3541 tcagtaccac cacatgctct acgctggtga cgttcgggtt ttttagtgcg tcatcaacgt
     3601 ttttcttcag cggaatacta cgcccggcgc gcacgccttc gtcggaagtg atcaccagtc
     3661 gtgagttgga atcaataatg cgcccggcaa cggcttccgg cgagaagccg ccgaaaatca
     3721 ccgaatgcat cgcgccaatg cgggcgcagg ccagcatcgc aaccgcggct tccggcacca
     3781 tcggcatata aatcgccacc acatcacctt ttttaatgcc cagctcgagc agggtattgg
     3841 cgaagcggca gacgtcgcgg tgcagctctt tatagctgat atgtttgctc tggctggcgt
     3901 cgtcgccttc ccagatgatg gcggtgcgat cgccgttttc ttgcagatgg cggtcaaggc
     3961 agtttgccgc cagattcagc gtgccgtcct cgtaccattt aatggacaca ttaccggggg
     4021 caaaggaggt atttttcacc ttctggtacg gtttgatcca gtcgagaatt tttccctgtt
     4081 cgccccagaa ggtatcaggt acgttaatag attgttgata catcgcctcg tactgctgag
     4141 ggtttatcag gcaacggtct gcgatgttgg caggaatggt gtgtttgtga atttggctca
     4201 tgcttttgtt ctccttgtag gatgttaata atatgtggca taagcgttaa atgtaggggt
     4261 attggcagtt ttgtttagta tttgggcgac agatcacgca aaagtagaat ggtgcaaata
     4321 aacggcaggg taatttttga aggtcaccaa caaaagttga ttaattcttt gaggaacatg
     4381 cagttatgca tgctgttgaa aaagaggaag atactgacta actctaaagt ggtatttcac
     4441 atgcacttac aattgattaa agacaacatt cacagtgtgg ttatttgtta cacatagggg
     4501 tgagcaatgt catgacagtg taggtgcggt tactgtcgtg aaaaacaata aaaaccgcca
     4561 ttgcaacaat ggcgcaattc ggatgaagcc cctatgacaa ggataaaaat aaacgcacgc
     4621 cgtatcttca gcttattgat tccttttttc tttttcactt ctgttcacgc tgaacaaacg
     4681 gcagctcccg caaaacctgt aactgtggaa gcgaagaatg aaacctttgc cccgcagcat
     4741 cacgatcaat atctctcctg gaaagccacc tcggaacagt cagagcgtgt tgacgccctg
     4801 gcggaagatc cacggctggt gatcctgtgg gcggggtatc ccttctcgcg cgattacaac
     4861 aagcctcgtg gacatgcctt tgctgtgacc gatgtgcgtg aaaccctgcg taccggtgcg
     4921 ccgaaaaacg ctgaagatgg tccgctaccg atggcgtgct ggagttgtaa aagcccggat
     4981 gtggcgcgtc tgatccagaa agacggcgaa gatggctact tccacggtaa atgggcgcgc
     5041 ggcggcccgg aaatcgtcaa caacttaggt tgtgccgact gccataacac cgcctcacca
     5101 gagttcgcca aaggcaaacc ggagttaacc ctttcccgtc cgtatgcggc tcgcgcgatg
     5161 gaagccattg gtaaaccttt tgagaaagcc ggacgtttcg accagcaatc gatggtttgc
     5221 ggtcagtgcc atgtggagta ttacttcgac ggcaaaaaca aagcggttaa attcccgtgg
     5281 gatgacggca tgaaagtcga aaatatggag cagtattacg acaaaattgc cttctctgac
     5341 tggactaact ccctgtcgaa aacgccaatg ctgaaagcgc agcacccgga atatgaaacc
     5401 tggacagcgg gcattcacgg taaaaacaac gtgacctgta tcgactgcca tatgccaaaa
     5461 gtgcagaacg ccgaaggcaa actctacacc gaccataaaa ttggtaatcc gtttgataac
     5521 ttcgcccaga cttgtgcgaa ctgccatacc caggataaag ctgccttgca aaaagtggtc
     5581 gcggaacgta agcaatcgat taacgacctg aaaatcaagg ttgaagatca actggttcac
     5641 gctcacttcg aagcgaaagc ggcgctggat gcaggcgcga cggaagccga aatgaagccg
     5701 attcaggacg atatccgtca tgcccagtgg cgttgggatc tggcgattgc ttcccacggt
     5761 attcatatgc acgcaccgga agaaggtcta cggatgctcg gtacggcgat ggataaagcg
     5821 gcggatgcgc gcaccaaact ggcgcgcctg ctggcgacca aaggtatcac ccatgaaatc
     5881 cagatcccgg atatctcgac caaagagaaa gcccagcagg ccattggcct gaacatggaa
     5941 caaatcaagg ccgagaagca ggacttcatc aaaacggtga tcccgcagtg ggaagagcag
     6001 gcacgtaaaa acggtctgtt aagccaataa ccccgttccg cctcgcaagg ggcggaaaac
     6061 acaatggagt gaatatgagc gtattacgtt cgttattaac tgctggggtg ctggcgtcgg
     6121 gcctgttgtg gagcctgaac ggaattaccg ctacccctgc ggtgcaggca tctggtgatc
     6181 gctacgaagt tacccagcag cgtaacccgg atgccgcctg tctggactgt cataaaccag
     6241 ataccgaagg tatgcatggc aaacatgctt ccgttatcaa cccgaataac aaattgccgg
     6301 tcacctgcac caactgtcac ggccaagcat caccgcaaca ccgcgaaggg gtgaaagatg
     6361 tgatgcgctt taacgagccg atgtataagg tcggagagca gaacagcgtc tgtatgtcct
     6421 gtcatctgcc ggaacagttg caaaaagcgt tctggccgca cgatgtccac gtaaccaaag
     6481 tggcgtgcgc cagctgccat tccctgcatc cacagcaaga tacgatgcag acgttaagtg
     6541 acaaaggacg gattaagatt tgcgtcgatt gccacagcga tcagcgcacc aatccgaact
     6601 ttaacccggc gtctgttccg ttgcttaagg agcagccatg acctggtctc gtcgccagtt
     6661 tctcaccggc gtcggcgtgc tggcagccgt cagcggcacc gcagggcgcg tggtggcgaa
     6721 aacgttgaat atcaatgggg tgcgttacgg catggtgcat gacgagtcgt tatgcatcgg
     6781 ctgtaccgcc tgtatggatg cttgtcggga agtgaacaag gtgccggaag gcgtctcgcg
     6841 cctgacgatt attcgtagcg agccgcaggg cgaatttcct gatgtgaaat atcgcttctt
     6901 ccgtaagtct tgccagcact gcgatcatgc gccgtgcgtt gacgtctgcc cgaccggtgc
     6961 gtcttttcgt gatgccgcta gcgggattgt cgatgttaat cctgatctct gcgtcggttg
     7021 tcagtactgc atcgccgcct gtccgtaccg cgtgcgcttt atccatccgg tcacgaagac
     7081 ggcggacaaa tgcgatttct gtcgtaagac caatttgcag gccggtaagc ttcccgcgtg
     7141 cgttgaagct tgcccgacga aggcgctgac gtttggcaat ctggacgatc ctaacagtga
     7201 gatttcgcaa ctgctgcgcc agaagcctac ttaccgctac aagctggcgc tgggaaccaa
     7261 accgaagctg taccgcgtac cgtttaaata cggggaggtg agccaatgac gcagacttcc
     7321 gcatttcatt ttgaatcgct ggtgtgggac tggccgattg ccatctacct gtttttgatt
     7381 ggtatttctg ccggtctggt gacgctggcc gtgctgttac gtcgcttcta cccgcaggcg
     7441 ggcggtgcag acagtacgtt gctgcgcacc acgctgattg tcgggccggg cgcggtgatc
     7501 ctcggtctgt tgatcctcgt cttccacctg acaagaccgt ggaccttctg gaagctgatg
     7561 ttccactaca gttttacctc ggtgatgtcg atgggggtga tgctgtttca gctctacatg
     7621 gtggtgctgg tgctgtggct ggcgaaaatc tttgaacatg atttgcttgc cctgcaacaa
     7681 cgctggttgc cgaagctggg gatcgtgcaa aaggttctga gcctgctgac gcccgttcat
     7741 cgcggactgg aaacgctgat gctggtgttg gcggtgctgc tgggggctta taccggcttt
     7801 ctgctgtcgg cgctgaaatc gtatccgttc ctcaataacc cgagcctgcc ggtgctgctc
     7861 ctcttctccg gcatctcgtc cggtgcggcg gtggcgctga tcgccatggc gatacgccaa
     7921 cgcagtaacc cgcattccac ggaagcgcac tttgttcacc ggatggaaat ccccgtggta
     7981 tggggtgaaa tcttcctgct ggtggcgttt tttgtcggtc tggcgctggg cgatgacggt
     8041 aaagttcgtg cgctggtggc ggcattaggt ggcggtttct ggacgtggtg gttctggctt
     8101 ggtgtcgccg ggctggggct gattgtgcca atgttgctca aaccgtgggt caatcgcagt
     8161 tccggcattc ctgccgtgct ggcggcgtgt ggggccagtc tggtcggcgt gttgatgctg
     8221 cgctttttca ttctctacgc cggacagttg acggtggcgt aagccagaaa agaggtggtt
     8281 tctggacgta ttccttcctg aagtcggttt tctggcgttg ttgttaagtc tcggggtcaa
     8341 cgtgttgacc ccgttgacgg cctttgcggg agtgcggttg cgctggcctg ccatgatgcg
     8401 actcacttgc atcggcattc tggcgcagtt cgcgctcctg ctgctcgcct ttggcgtact
     8461 gacgtattgt tttctcatca gcgatttctc ggtcatttat gtcgcccaac atagctacag
     8521 cctgctgtcg tgggaactca aactggcggc ggtgtggggc ggtcatgaag gttcgctgct
     8581 gctttgggtg ctgctgcttt ccgcctggag cgcgctgttt gcctggcatt atcggcagca
     8641 aagcgatccg ctatttccgc tgacgctagc cgttttatct ctcatgctcg ccgcactgct
     8701 actgtttgtg gtgctgtggt ccgatccctt cgtgcggata tttccaccag caatcgaagg
     8761 ccgcgatctc aatccgatgc tgcaacatcc cggtcttatc tttcatccac cgctgcttta
     8821 ccttggctat ggcggtttga tggtagcggc gagcgtggcg ctggcgagtc tactgcgcgg
     8881 cgagtttgat gcggccagcg cccgaatttg ctggcgctgg gcgttacctg gctggagtgc
     8941 attaacggcg gggatcatcc tcggttcctg gtgggcctac tgcgaactcg gctggggcgg
     9001 ctggtggttc tgggatccgg tggaaaacgc ctctttatta ccctggcttt ctgccactgc
     9061 gctgctgcac agtttgtccc tgacacgcca gcgggggatt ttccgccact ggtcgctgtt
     9121 gctggcgata gttactctga tgctgtcgct gctgggcacc ttaattgtcc gttctggcat
     9181 tctggtttcg gttcatgcgt tcgcactgga taacgtccgc gccgtgccgt tgttcagcct
     9241 gtttgcactg attagccttg cgtctttggc tctgtatggc tggcgagcgc gggacggtgg
     9301 cccggcggtg cgtttttcgg ggttatcgcg ggaaatgtta atcctcgcta cgctgttgct
     9361 gttttgcgca gtgctactga tcgtgctggt gggaacgctt tatccgatga tttacggcct
     9421 gctgggctgg ggacgcctct ccgttggcgc gccgtatttt aaccgcgcga cgttaccgtt
     9481 tggtctgttg atgctggtgg tgattgtgct ggcgacgttt gtctctggca aacgcgtgca
     9541 gcttccggcg ctggtagctc atgctggcgt gctgttattt gccgcgggga tcgtggtttc
     9601 cagtgtcagt cgtcaggaga acagcctgaa tttacagccg ggtcagcagg tgacgctggc
     9661 aggatacacc ttccgttttg agcgactcga tctgcaagcc agaggcaatt acaccagcga
     9721 aaaagcgata gtggcgctgt ttgaccatca gcaacgcatt ggtgaattaa cgccggagcg
     9781 gcgtttttat gaagcacgcc gtcagcaaat gatggaaccg tcaattcgct ggaacggcat
     9841 ccatgactgg tatgcggtca tgggtgaaaa aaccagagcg gatcgttacg cttttcgttt
     9901 gtatgtacaa agcggtgtgc gctggatctg ggggggagga ttgttgatga ttgcgggcgc
     9961 attgttaagc ggatggcggg ggaagaagcg cgatgaataa agggcttctc acgctactgc
    10021 tgttatttac ctgttttgcg cgcgctcagg ttgtagacac ctggcaattc gccaatccgc
    10081 aacaacagca acaggcgtta aatattgcca gccagttacg ttgtccgcag tgccagaatc
    10141 aaaacttact cgaatccaat gcgccggtgg cggtcagtat gcgccatcag gtttatagca
    10201 tggtgtcgga ggggaagaac gaagtcgaaa tcattggctg gatgaccgaa cgctacggag
    10261 attttgttcg ctataaccca ccgttaacgg gtcagacgct ggtgttgtgg gcgctgccag
    10321 tggtgttgtt attgctgatg gcactgatcc tctggcgagt gagggctaag cgatgaaaca
    10381 gcctcaaata ccggtgaaaa tgctgacaac actcacgata ttgatggtat tcctctgtgt
    10441 cggcagttat ctgttaagcc caaaatggca ggcggtacgt gcggagtatc agcgtcagcg
    10501 cgatccgcta catcagtttg ccagccagca aaccccggaa gcgcagcttc aggcattgca
    10561 ggataaaatc cgcgctaatc cacaaaacag cgaacaatgg gcgttactgg gcgagtacta
    10621 tctgtggcaa aacgattaca gcaattcgct gctggcgtat cgtcaggcgt tgcaactacg
    10681 tggtgagaac gctgaactgt atgcggcgct ggcgacggtg ctttattacc aggccagcca
    10741 gcatatgacc gcccagactc gcgcaatgat cgacaaagcc ctcgcgctgg actctaacga
    10801 aatcaccgct ctgatgctgc tggcttccga tgcgtttatg caggcgaatt acgcgcaagc
    10861 catcgaacta tggcaaaaag tgatggatct caactcaccc cggatcaacc ggacacagct
    10921 ggtcgagtcg attaatatgg caaaactgtt gcagcggaga tcggattaat catctggtga
    10981 tgaagattat gtcattgagc tgcataaaaa ataatcgaat gaacatatgc caaaaataat
    11041 caccaatcag tattattgca gattaaacaa ataaaaatct ttccataaca aatggttatt
    11101 cattaatcct gccataaact cgcgttatta tgcattaatg cagcgaaaag ctctgttgtt
    11161 aaagggttgc gcaacatacc gcgcaaatga tactgatcat aagcgttaaa aaaatctaca
    11221 aaccaacgca acacaattca tgccctggca gtatgtcacg ttctcgcgtt tctgaacggg
    11281 gaacggcgct ccattgagga agttattctg atgaaaaata taaaattcag cctggcctgg
    11341 cagattctgt ttgctatggt gctgggcatt ctcctgggaa gctacctgca ctaccatagc
    11401 gacagccgcg actggctggt cgtcaatttg ctctctccgg cgggtgatat cttcatccat
    11461 ctgatcaaaa tgattgttgt gccgattgtg atctccacgc tggtggtggg tatcgcgggt
    11521 gttggtgatg ccaaacagct cgggcgtatt ggcgcgaaaa ccattattta cttcgaggtg
    11581 atcaccaccg tcgccatcat tttggggatc actctggcga acgtcttcca gcccggtgcc
    11641 ggggtggata tgtcgcagct ggcgaccgtc gatatctcga aatatcagag cactacggaa
    11701 gcggtacaaa gcagttccca cggcattatg ggcacgattt tgtcgctggt gccgacgaac
    11761 attgtggcgt cgatggcgaa aggcgaaatg ctgccgatca tctttttctc ggtgctgttt
    11821 ggtctggggc tttcttccct gcccgcgacg catcgtgaac cgctggtgac cgtgttccgc
    11881 tccatctctg aaaccatgtt taaagtgact cacatggtga tgcgttatgc accggtgggt
    11941 gtgtttgcgc tgattgcggt gacggtggct aactttggtt tctcgtctct gtggccactg
    12001 gcgaaactgg tgctgctggt gcatttcgcc attctgttct tcgcgctggt agtgctggga
    12061 attgtggcac gtctgtgcgg gttaagcgtc tggatcctga ttcgtattct gaaagatgag
    12121 ctgattctgg cgtactccac tgccagctct gaaagcgtac tgccgcgaat tattgagaag
    12181 atggaagcct acggagcacc ggcgtcgatc accagcttcg tggtgccgac cggttactct
    12241 tttaaccttg atggttcgac gctgtatcaa agtattgccg ctattttcat cgctcagctg
    12301 tatggcattg acctgtccat ctggcaggaa atcattctgg tgctgacgct gatggtgacc
    12361 tcgaaaggga ttgctggcgt ggctggcgtg tcgtttgtgg tgttgctggc aacgctgggt
    12421 agcgtaggta ttccgctgga aggtctggcg tttattgctg gtgttgaccg tatcctcgac
    12481 atggcgcgta ctgcgctgaa cgtggtgggt aatgcgctgg cggtgctggt gattgccaag
    12541 tgggaacaca aatttgaccg caaaaaggcg ctggcttatg agcgtgaagt gctgggcaaa
    12601 tttgataaaa ctgcggatca ataattgaag attgccgggg atatcccccg gcaatgtgtg
    12661 gatgcctgat gcgacgcttg ccgcgtctta tcaggcctac cttaaccgtt ggttaatttt
    12721 tcaaactctt cacacccggt atcaaaccct tccatacagc tcaggttcaa ccagtgcagc
    12781 gccttttgtt tgttcttctc gataaaacct tcctcaccgt ttaagaacat cattcccgcc
    12841 cagtactcgg aataaccggt tcgggaaatt gcagagctgc gtttgaaata ccaggttgcc
    12901 ttgtcatcgt cggccttaat gcccacgccg ttggcgtaaa tcaaaccaag cagcatttgg
    12961 gcatcgacgg cggagtcatt ctccagatct tccgaggcgt tttctaacag cgaaatcgcc
    13021 tttggataat ccggtttacc cgcttgagta tttaccagaa tatgcgccag cgttacttca
    13081 ccttctttac tgcccgcttg cgccgctttc tctgcaagaa cttttgcttg tggatagtcc
    13141 agactgaccg gattggtgat tttgatctgc gccaacagcg cgcaggcatc agcatcaccg
    13201 ctgtcggcgg ctttctgtgc ccaatattcg gctttgctca aatcgccgga gctaaaccag
    13261 ctgtcggcaa gaaaatattg tgcacgtcgg tccccggcct ctgctgcctt taaatactgg
    13321 ctgccaggct cggagtcgtt cgcgtgggca aagaatgtca aaaacaacat caatgcgata
    13381 atttttttca ttttgataat acgattgggt agggagtaac cagtataaag agttcgaatg
    13441 gataaaaaaa cagcctccga aaggaggctg tagaaaggac tgtattacgc cagtgccgct
    13501 tcgcgcaggc gagttttcaa cttgttgtac tcgtcaatca cgtactgctc ggcggcgcgc
    13561 tgatcggaga tcggctcgac gcgcacggcg cagtatttgt attccggcgt tttcgtaatc
    13621 gggcttaagc tttcggtaac cagctcgtta caggcaccaa tccaccactg gtaggtcatg
    13681 taaatcgccc ctttgttcgg acgatcgctg acctgcgcac gggtgataat tttgccttta
    13741 cgcgagtgca ctcaaaccaa tgcctcatct tcaataccca gacgtttggc gtcttcggta
    13801 ttgatttgtg cgtagccagg ttcatcagcc agcgccgcca gtgccgcaca gttaccggtc
    13861 atcgaacggc aagagtagtg accaacttca cgcaccgttg acagtaccat cgggtactcg
    13921 tcggtgagtt tgtcgattgg cgctacccag tcgcaggtga agaactgcgc cagaccgttc
    13981 ggggtatcaa acttctcttt aaacagataa gaagtcccct gatcggcatc tgaagtatcg
    14041 cggcaaggcc actgaatgaa gcccagttcg cccattttct cgtaagtcgc accgtagaaa
    14101 tccgggcaca gatgacgcaa ctcatcccag atctcctggg tgttgttgta gtgcatcgga
    14161 taacccatac gggtggcgat ttcactgatg atttgccagt ccgttttcag atcccatttc
    14221 ggttcaactg ctttgaagaa acgctggaag ccacggtcag ccgcagtaaa cacgccttca
    14281 tgctcgcccc acgacgttga cggcaaaatg acatccgccg ccgacgcggt tttggttata
    14341 aagatgtcct gaacgatgac cagttccaga tcttcaaagg ctttacgtac tgccgacagc
    14401 tccgcatcag tttgcagcgg atcttcgccc ataatgtacg cggcacgcac ttcgccatgc
    14461 gctgcgcggt gcggcagctc gctgatgcga tagccggtat gagcaggcag gctttccacg
    14521 ccccaggctt tggcgaattt ttcgcggtta gccggatctt tcacgtactg gtatcccgga
    14581 tacgtatccg gcagcgcgcc catatcgcag gcaccctgca cgttgttctg accacgaacc
    14641 gggtttacac ccgcatgcgg cttaccgagg ttactggtca gcatcgcgag gctggtcaga
    14701 gaacgcacgg tttccacgcc ctggtagaac tgggttacac ccatgcccca caggatggcg
    14761 gcgcttttcg cctgggcata catccgtgcc gcctgacgaa tctcactggc gctgacaccg
    14821 gtgatatctt caaccgactc cggcgtgtag ccttcaacga ttttacgata ctcttcaaag
    14881 ccttctgtac gggaagcgac gaacgctttg tcgtacagat tttcttcaat aatgacatgg
    14941 cccatcgcat tcaacagcgc gatgttcgag ccgtttttca gtgcaatgtg catgtcagca
    15001 atgcgcgcgg tttcaatttt gcgcggatcg cagacgataa ttttcgcccc gttacgttta
    15061 gcgttaatta cgtgattcgc cacgattggg tgggaatccg ccgggttgta cccgaaaacg
    15121 aacactaaat cggtattatc aatttcgtta atagcattgc tcattgcgcc attaccgacc
    15181 gattggtgca gacctgcaac cgatgggccg tgtcagacac gagcgcagca gtcaacgtta
    15241 ttggtaccaa taacggcgcg cgcaaatttt tgcattacat agttggtttc gttacccgta
    15301 ccacgcgagg agccggtcgt ctggatggca tccggaccgt acttctcttt gatggcgctc
    15361 aggcgctcgg caacgtaatt cagtgcctca tctcaggaaa caggttcgag tttgccgcca
    15421 cgctggcgac ggatcatggg ggttttcagg cgcggggtca ggatctgggt atcgttaatg
    15481 aaatcccagc cataataacc cttcagacac agggtaccct ggttggtttt cccctgcgct
    15541 gcctccgccc ggacgatttt gccgttatcg acgaccaggt tgattttgca acctgatgcg
    15601 caataggggc aaaccgtgac gacttttttc atcggtctcg ctccagttaa tcaaatcacg
    15661 catacgcgct ctcgactaca gtatgcatct tttatgccac attttatttg gggtcattcc
    15721 ctgatattac gggcactatt tattcaaaac tctgacgaaa aacaggctgt cgtcagtttt
    15781 gacgtgacga aacgaaatac cgcgtgacag ccatcacgcg gcagacattt tattttttct
    15841 cgacgacggg agccgcctga tacccgccgc ccagcgattt catcaactga atgctttgga
    15901 tcacccggcg gctgtccagc atcagtaatg acatctcttc ggcaagtact ggcaaccggg
    15961 cttcagtggc ctgtaagcgg ctggttaagc cgcgctgata ggcggcctcg gcagcgcgct
    16021 gggtgaagcg cgttgcttcc acgtgttcag cctgcatttc tcgctcgtcg ttgagcgttt
    16081 gcagacgcgt gccgttgacg gcaacgtcac gcaccgcgtt cagtactgac tggttgtaac
    16141 gttcaatcat catgttgctg gcggcgcgcg tgccttcgag attggcattc aaccgtccac
    16201 cgtcaaacag cggcaatttc agacccggga tgaagttgaa ctggcgactg gtttttttga
    16261 ataaggtatc caggtggatg gcatcaaggc cgaaaaacgc tttgatatca aagctcggat
    16321 agaacaacgc ccgcgcggaa tccacctgat ctaatgacgc ctgaacatac cagcgcatgg
    16381 cttgcagatc cgggcgtctg gcgagcaact cataagagag tgtcgccgga atgccggtct
    16441 ggactcgtgg taatgccaca ggtttgatct ccggcatatc gctggctccc gcgccaatca
    16501 atgcgcgcag agattctctc gtttcggtga tttgcccttt gacggcagca atttgtttat
    16561 cgaccgccag aatctgtgcc cgcgcgccgt ggaaaggcac ttgtgcttcc agaccgtgcg
    16621 ccactttact ttggtgcgct ttcaccgcgt aatcaatcac atcgcgagtt tgttctaaca
    16681 gatcgagcat ctgatagctg gcctgcatac tgtaataaag ctgcgctacg cccgtggtca
    16741 gcgatagctc tactgctgcg gtttctgcca gcgcggcatt atgcgcgcca atggcggcgg
    16801 caaccgctga gcgatgcaca ccccacaaat caagatccag tccggcaaac aaacctactg
    16861 tggcttccgt atagtacggc ccgtccatcc ccagtgcggg cgcatccatc gcataagggc
    16921 ttaaaaagcc gttcgccgag acacgttggc ggttaagcat ccctaacgct gccacctgca
    16981 actgtgaacc ggcatctaac aaatcggcct gcgattgcgc tttttcttcc cgcagtttcg
    17041 cttcggcgag ggtgtgtgaa ccacttagcg tccgttggat cagggcatcc aactgcgggt
    17101 cattgagttg tttccaccac tgcgcctgcg gccagccgga gctggcaaga tgaatatcat
    17161 cggccagttt gatttgttcc ggtttgagct gttgatgagg cgcagaatcc ttacgtacca
    17221 gggcacagcc ggaaatcagc gtcgtgctgc cgagaatgct gcacaacagc agacgtgaaa
    17281 gttgacgatt gatcattgcg tggctccttg tgcctgttcc gtggcgggcg ttaatgccgg
    17341 ggctgtccag tctggcaagc gggcgaaaag ctggcagacg tgttgctcct gttttaataa
    17401 ggtgggcagg atggcctgcg aggtgggtgt ctcttcaagc gttatttgag gagaacggct
    17461 gagtgcggtt gccagaccgg cagcgtattt ctccagggcg tcggcaaact gacctgctct
    17521 gtccggctgt agtgcgttat ccagcgcctg cgccgaattc caggtggcat cccaggcgtg
    17581 aaggatatcg cggccctgac gaataaccgt ttgcgaacgt tcaatcagta aggcgcgttc
    17641 ttcgctgtcc agttgacgct ccagcgccac gcgttggcac atctcttcac aggcattaaa
    17701 cgccgcatgc agaccgatac gaatttgcag ataagtgcgc agagccgtga cttcctgctg
    17761 gcgtgggatc cgcattactt tacttaacat acccagcgcg ccagccagtt tttgcggcag
    17821 cgtgcgcgct tcactttcag gccagacaaa ggtgtaaatc atcgcggaca ccacggtacc
    17881 aatgatgata cccagggcgc gatcgcgaat ttccaccagg tcgtacactg ggccaaaaac
    17941 gttttcgagc gtggcgagcg cgaaggtgac caccatctgt gtgccgatat aagaagagcg
    18001 ttcagagctg gtggcaatcc atgcgcccaa caggaaaatc ggtgccagca caaacagcaa
    18061 ttcgacaata ttgtccagcc agggcatgac cagtagcgtg aataacagcg ccagaatcgc
    18121 gccgcaaaag gccccgccaa aacgcagcac catcttctgg tacgacgaac cgacatttgg
    18181 attagcgacg atcacgcagg tcagcataca ggtgtgaatg ccttcccagt ccacgccgct
    18241 gtaaaaggtg taacagatca aacaggcgag caatgttttt accgcgtagc gtatatagtc
    18301 tggattggta aaagcatcgg cgaccattga tggcggtttg gcggcgggcg ttggcggcgt
    18361 attcgggttc atctggccca gttgtaacaa cgtctggcag atattctcca ggttacattc
    18421 ccgtgccgcc accgcttcac tttcactgag ccgccagtcg ctttgccagc actgaccttc
    18481 agcaacggca tgctgcagct tgttgatttc tgaggctaat ttttgccgaa attcaataat
    18541 tgcctgagaa tcagcaaaag aggtgggatc gtagcgattc agcgtcgagt aaatgtaggt
    18601 taccgttgcc acgcagcttt gccaccatgc gctttgagtt cgccagttgg catcgtccgc
    18661 gagacaaaag acattcagtt tttgcagcgc cagcgcctct ctttcaatcc gcgtttcggg
    18721 tagcggtgcg aggctgtccg tcaggtggct aatggcatca tcaagccgat cattaagcgc
    18781 ttgatgcatt tgcgtaatgg cacgattggg aaaccacagc acgccgatta acgtcatcag
    18841 caaggttgga tagaggccaa caacgataca ccacagcgtt aagcgcacga ctacttccgg
    18901 atagtcgagc atggcgggga aggtttgccc gtaaatggcg acagcgaaaa agaccagtcc
    18961 cagccgatgg gtgcgcatca aaaacatgca gcccatcagg atcggcccgg cgatgatcaa
    19021 tcggatcaac ggttcgccgt atgaccattt gtagatcaaa aacaggctgc cgatctccag
    19081 caccgtggca accacaaaca agatcgccac aaatttggtg taaaacgcgt tcgactgaat
    19141 accgtaaaac agcactgcca gcgataacgc cacaaaaggg atctcaaaag tcatcgagat
    19201 cagaatcacc agcaggcagc ctacccagag ttgcacgatc tgcggcacgc ggccgggtcg
    19261 ccgctcgctt aactcttcat gaaagaacgc cagcagcctg accaccggta atggcaggga
    19321 gttgagcgcg ctcattattg cggctcaaga ttagcgactg ccgaagcgcc gatgcggaac
    19381 atttccgggt caggtttatc gaccatgatt ttgaccggaa aacgctgggc aacgcggacc
    19441 cagttaatag aacgagacac ttttggcagg ccgcccagca ccaggccgcc gtcatccggt
    19501 agcacgccgt agccaatcga atccacttta ccctcgaagg ttttgccgct gtcactcatc
    19561 aggcgaatcg ttgcgggtgt acctgagcga atatttttca gatcggtttc gcggaagttg
    19621 gcgatcacgt accagtgacg agtgtcgatg agggtaaaaa tagggcgcat ggcagaagca
    19681 aattgcccga cggaggtttt gagggaaatg atccggccat caaatggcgc gcgaacggtc
    19741 gtcatttcca gatgtagttt ggtcagggca atatccgctt cgaccgccgc acgttgcgcc
    19801 accagcgcat cgacgccgct gacggcgctg gcggctgact gcgcctgtaa caatacggca
    19861 ttaagatccg cttctgcggc gcgctgcgcc gttcttgcac ggtcaacgtc ttctgctgag
    19921 acaaaaccct ctttcagtaa tggctcggtg cggcgtaatg tatctgtggc ctgtttcgcg
    19981 gcggcacggg ctttttctac cgtggcatta accgagtcgg caccaaactg ttgggcgtca
    20041 acgctacgct gggtgagcat aatttgctta tccagcgccg cgagggaggc ttcggctttc
    20101 gccaggttgg cttcgtacgg gcgcgggtcg atgcggaaca gcaaatcgcc ctgtttcacc
    20161 gcctggttgt cggtgaccgc cagttctaca atgcgaccgc tgacttccgg caccacatca
    20221 atggtatctg ctgacgcgta agcgtcatta gttgatggcg cactgtctac gcgccagata
    20281 acgaaaacaa gggcaaccag cgccaacgca accactaaca gagcagggaa tttactgcga
    20341 ggtgcttttt tcggcgtact ttccatgagc gcgtctcaaa tagattagaa aaatgccagc
    20401 cagaacagca tggcgtagag agcaaaaagg gcggtataaa taattccgac aaatgccaga
    20461 ttgatatttg cccg
//
LOCUS       DAZSIO010000065        20403 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZSIO010000065 DAZSIO010000000
VERSION     DAZSIO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.065"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            75..227
                     /gene="hokA"
                     /locus_tag="ACHX5T_002139"
     CDS             75..227
                     /gene="hokA"
                     /locus_tag="ACHX5T_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR8467333.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(204..275)
                     /gene="ysaE"
                     /locus_tag="ACHX5T_002140"
     CDS             complement(204..275)
                     /gene="ysaE"
                     /locus_tag="ACHX5T_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsaE"
                     /protein_id="HFR8467334.1"
                     /translation="MRNAVSKAGIISRRRLLLFQFVG"
     gene            complement(414..626)
                     /gene="cspA"
                     /locus_tag="ACHX5T_002141"
     CDS             complement(414..626)
                     /gene="cspA"
                     /locus_tag="ACHX5T_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR8467335.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(907..1197)
                     /locus_tag="ACHX5T_002142"
                     /pseudo
     CDS             complement(907..1197)
                     /locus_tag="ACHX5T_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1631..2341
                     /locus_tag="ACHX5T_002143"
     CDS             1631..2341
                     /locus_tag="ACHX5T_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR8467336.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="ACHX5T_002144"
     CDS             complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="ACHX5T_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR8467337.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3469..4129)
                     /locus_tag="ACHX5T_002145"
                     /pseudo
     CDS             complement(3469..4129)
                     /locus_tag="ACHX5T_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
     gene            4282..6480
                     /locus_tag="ACHX5T_002146"
     CDS             4282..6480
                     /locus_tag="ACHX5T_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFR8467338.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6449..6889)
                     /locus_tag="ACHX5T_002147"
     CDS             complement(6449..6889)
                     /locus_tag="ACHX5T_002147"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR8467339.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6886..7449)
                     /gene="tag"
                     /locus_tag="ACHX5T_002148"
     CDS             complement(6886..7449)
                     /gene="tag"
                     /locus_tag="ACHX5T_002148"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR8467340.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7607..8305
                     /locus_tag="ACHX5T_002149"
     CDS             7607..8305
                     /locus_tag="ACHX5T_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR8467341.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8534..9742
                     /locus_tag="ACHX5T_002150"
     CDS             8534..9742
                     /locus_tag="ACHX5T_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8467342.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10066..11757
                     /gene="eptB"
                     /locus_tag="ACHX5T_002151"
     CDS             10066..11757
                     /gene="eptB"
                     /locus_tag="ACHX5T_002151"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR8467343.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11849..11925
                     /locus_tag="ACHX5T_002152"
     tRNA            11849..11925
                     /locus_tag="ACHX5T_002152"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11883..11885,aa:Pro,seq:cgg)
     gene            12669..14276
                     /gene="dppA"
                     /locus_tag="ACHX5T_002153"
     CDS             12669..14276
                     /gene="dppA"
                     /locus_tag="ACHX5T_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR8467344.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14584..15603
                     /gene="dppB"
                     /locus_tag="ACHX5T_002154"
     CDS             14584..15603
                     /gene="dppB"
                     /locus_tag="ACHX5T_002154"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR8467345.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15613..16515
                     /gene="dppC"
                     /locus_tag="ACHX5T_002155"
     CDS             15613..16515
                     /gene="dppC"
                     /locus_tag="ACHX5T_002155"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR8467346.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16526..17509
                     /gene="dppD"
                     /locus_tag="ACHX5T_002156"
     CDS             16526..17509
                     /gene="dppD"
                     /locus_tag="ACHX5T_002156"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR8467347.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17506..18510
                     /gene="dppF"
                     /locus_tag="ACHX5T_002157"
     CDS             17506..18510
                     /gene="dppF"
                     /locus_tag="ACHX5T_002157"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFR8467348.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18540..19811)
                     /locus_tag="ACHX5T_002158"
     CDS             complement(18540..19811)
                     /locus_tag="ACHX5T_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR8467349.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20287..20394
                     /locus_tag="ACHX5T_002159"
     CDS             20287..20394
                     /locus_tag="ACHX5T_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR8467350.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         4890 a         5049 c         5370 g         5094 t
ORIGIN      
        1 agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac
       61 acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca
      121 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      181 agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcgacgg
      241 gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccat atttattctt
      301 cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa
      361 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      421 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      481 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca
      541 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      601 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      661 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      721 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      781 accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt
      841 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      901 aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      961 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1021 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcataactg
     1081 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1141 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1201 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1261 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
     1321 tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttat
     1381 cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta aatctctggc
     1441 tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1501 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1561 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1621 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1681 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1741 tatcgacttc ctgcaaaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1801 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1861 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
     1921 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc
     1981 gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2041 tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt
     2101 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2161 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2221 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2281 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2341 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcagc ttagtccgcg
     2401 acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2461 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2521 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2581 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg
     2641 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2701 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2761 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2821 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcacacgt
     2881 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2941 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     3001 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3061 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3121 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3181 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttctgcaaaa
     3241 attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3301 tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3361 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3421 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3481 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3541 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3601 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3661 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3721 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggagcgc tgctgctgtc
     3781 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3841 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3901 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     3961 cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     4021 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     4081 cagagcgcca ctcactacgg cggcaagtaa gataaacacg tttcttcatt gttaatcctt
     4141 aataaccttt ttattctttg tcacgggttc cgtggcggga gattatgccg cgtgaacatg
     4201 aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct caatacatct
     4261 aacctttcag gagtcttcgg tttggccaac tcatcctcac gatattccgt tctgactgcc
     4321 gcccactggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag ctcgcgtggc
     4381 gcgttagcca caggaatgga aaactccttg cagagcgcgg ttcgcgacca ggttcacagc
     4441 aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc ggaaaacccg
     4501 caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc gctggcaggc
     4561 ggttataccg ggcatctggg ggattattcg accggtgcgg cacaggcgat catgccgtat
     4621 gtcgtaggtg gcagcgaagt ttatcaacag caaaccagtt ggccgctggt gctggaacat
     4681 agcgatgtcg tggtgttgtg gagtgctaac ccactcaata cgctgaaaat tgcgtggaat
     4741 gcatccgatg agcaggggct ttcttacttt tccgcactgc gtgacagtgg gaaaaagctg
     4801 atctgcattg atccaatgcg atcggaaacc gtcgatttct ttggcgataa aatggagtgg
     4861 gtggcaccgc acatgggcac cgatgttgcg ctgatgctgg ggatcgccca tacgctggtg
     4921 gaaaatggtt ggcacgacga agcgtttctg gcgcgttgca ccacaggtta tgccgtcttc
     4981 gcctcttatt tgctgggcga gagtgacgga atagcgaaaa acgccgaatg ggcggcagag
     5041 atttgtggtg ttggcgcagc gaaaatccgc gagctggcgg ctattttcca ccaaaatacc
     5101 accatgctga tggcaggctg gggaatgcag cgccaacagt ttggtgagca aaaacactgg
     5161 atgatcgtca cgctggcagc aatgttgggg caaatcggca cacccggcgg cggttttggt
     5221 ctttcttacc attttgccaa tggtggtgac cccacgcggc gttctgcggt gctctcttcc
     5281 atgcagggca gcttgccggg tggctgcgat gcggtggata aaatccctgt tgcccgcatt
     5341 gttgaagcac tggaaaaccc tggtggcgca tatcaacaca acggtatgaa ccgacatttc
     5401 ccggatattc gttttatctg gtgggcgggc ggtgccaact ttactcatca tcaggatacc
     5461 aatcgtctga tccgtgcctg gcaaaaaccg gagctggtgg tgatctctga atgcttctgg
     5521 acggcggcgg cgaaacacgc ggatattgtt ctgcctgcga ctacctcttt tgagcgtaat
     5581 gatctcacca tgaccggtga ttacagtaat cagcatctgg tgccgatgaa gcaagtggtg
     5641 ccgccacgct atgaagcgcg taatgatttt gatgtttttg ccgagttgag tgaacgttgg
     5701 gagaagggcg gctatgcgcg gtttacggaa ggaaaaagtc agctgcaatg gctggaaacg
     5761 ttttataacg ttgcccggca gcgcggggca agccagcagg ttgaattgcc gccatttgct
     5821 gagttctggc aagccaacca gttaattgag atgccggaaa acccggacag cgagcggttt
     5881 attcgcttcg ctgatttttg ccgcgatccg ctggcgcatc cgttaaaaac cgccagcggc
     5941 aagattgaaa tcttctcaca gcgtattgcc gattacggtt acccggattg ccctgggcat
     6001 ccaatgtggc tggagccgga cgaatggcag ggcaatgccg aaccagaaca gttgcaggta
     6061 ctttctgccc atccggcgca ccgcctgcac agccagctga attacagttc tctgcgcgaa
     6121 ttgtacgcgg tagcaaaccg tgagcctgtc accattcatc ctgacgatgc tcaggcgcgc
     6181 ggcataaccg aaggggatat ggtgcgggtg tggaactccc gtgggcagat ccttgccgga
     6241 gcggtcatta gcgagggaat taaacctggc gtgatttgca ttcacgaagg ggcatggccg
     6301 gatctggatt taaccgctga cggtatttgt aaaaacggcg cagtgaacgt gctgaccaaa
     6361 gatctcccca gctcgcggct ggggaatggc tgtgcgggta atacgacgct ggcatggctg
     6421 gaaaaataca acggtccgga actgacactt acagcgtttg aaccaccggc cagctcataa
     6481 tccaggtggg tagttgggtt tcatcctgcc atgcgcaatc gacaatgtga aaaccctgcg
     6541 cacggtaaaa atctatcgcc ggttgatttt tttgataaac ctccagcatc agatggggat
     6601 agcgctgctg cacatactgc atcagcgcct taccaatacc gcgtctgacg gccttcggtg
     6661 cgacaaacat tgctgccaga aatcggcctt ccataatgct gacaaaaccg agaagcttac
     6721 cgtcctcttc ccagacccag ttttgcgcgt tggcaagata ggcatcccgc accagcggaa
     6781 tgcagtcacg ccagtaattc gcttttataa agggatgccc ccaggttgta ctttccagcc
     6841 acagttcgag gatcgcgggg agttctgaac gttgcgcttc ccgaatcatg gtttatttcc
     6901 cagatagcaa cagcagccaa ccacatgatc attcaccagc ccacatgcct gcataaagga
     6961 gtaacagatt gtggtgccga caaacttaaa accacgtttt ttcagtgcct tagatagggc
     7021 gtcggaggcg gacgtagatg tgggaatttc gctcaacgtt gtggcttgtg tcacctgtgg
     7081 ctgatgattt acaaacgacc agacaaagtc gggaaacggt tcgccgttct gttccatttg
     7141 caggtacgcc cgcgcattac caataattgc ctgaattttc cctcgatggc ggataatccc
     7201 ggcgtcctgt accagtcttt cgacatcctc ttcctgcatt gctgcgacct tcaccggatc
     7261 gaactgatga aagtaggcgc gatagttttc gcgttttttg aggacggtga tccacgataa
     7321 cccagcctgc tgcccttcaa agcagatcat ttcgaacagt tttttactgt cagtttcagg
     7381 cacgccccac tcattatcat ggtaggcaat ataaagcggg ccctgactca cccagccgca
     7441 acgttccata ctttccctcg ctatgtaccc gatcgtaaaa atttcagccg cttttcttga
     7501 cgcatcggtg aaaaagtcaa tatttaatac agggctacta tgcccgatat ccttcataca
     7561 atgacaataa tattgccgaa ttcggctctt ccctggagtc gtgttaatga tcataaaaaa
     7621 aagcggtggt cgctggcagc taagcctgct ggcgagcgtg gtaatcagtg ccttttttct
     7681 caacacagct tacgcctggc aacaagaata tatcgttgat acgcaacccg gacattccac
     7741 agagcgttac acctgggata gtgatcatca acctgattac aacgatattt tgtcgcaacg
     7801 tattcaaagt agccaaaggg cgctgggact ggaagtcaat ctggcggaag aaactcctgt
     7861 ggatgtgacc agcagtatga gtatgggctg gaattttcct ttatatgaac aggttacaac
     7921 cggcccggtc gcggcattac attacgatgg cacaaccacc tcgatgtata acgagtttgg
     7981 cgacagtact accacgctga ccgatccgtt atggcatgcc agcgtgagta gcttaggctg
     8041 gcgtgttgac tcccggcttg gcgatctccg accctgggcg caaatcagct ataaccagca
     8101 atttggcgag aatatctgga aggcgcaatc aggcctgagc cggatgacgg cgacaaacca
     8161 gaacggcaac tggctggatg tcaccgtagg cgctgatatg ttgctcaatc aaaatattgc
     8221 cgcctatgct gcgctaactc aggcagaaaa taccactaat aatagcgact atctgtatac
     8281 gatgggggtt agcgccagat tttaacgtaa cagtcacaat tgaaaccatt gaataacaac
     8341 agttgtggcg atagtgggtg ctaacttacc aaataataaa tttggtgaat aattgtcgcg
     8401 tcattcattc ctgaactaag gcatttcatt ccgttctgat ggcatttcat gccgtttttc
     8461 cccaagcata aaatgcactt cgttatggtt gtcggcagag atttttcctt tttattactg
     8521 caggaatact gccatgacac cttcaaatta tcagcgtacc cgctggctga cactcatcgg
     8581 tactatcatt acccagtttg cgctggggtc ggtttatacc cggagcctgt ttaatggcgc
     8641 gctttctgcc aagctggatg cgccggtaag ccaggtcgct ttctctttcg gcttgttaag
     8701 tctggggctg gcaatttcgt cttctgttgc gggcaaattg caggaacgtt ttggcgttaa
     8761 acgcgtcacc gtggcttccg gcattttgct gggattaggc ttcttcctga ccgcgcattc
     8821 caacaacctg atgatgctgt ggttaagcgc cggtgtgctg gtcggtctgg cggatggcgc
     8881 gggttacctg ctgacgctct ctaactgcgt gaagtggttc ccggagcgta aaggtctgat
     8941 ctccgcgttc gctatcggtt cttatggtct gggtagcctg ggtttcaaat ttatcgacac
     9001 gcagctgctc gaaacggtgg gtctggaaaa aacctttgtg atttggggag cgattgcgct
     9061 ggtgatgatt gttttcggcg caacgttaat gaaagacgca ccaaaacagg aagtgaaaac
     9121 cagcaatggt gtggtggaga aagattacac gctggcagag tcgatgcgta aaccgcagta
     9181 ctggatgtta gcggtaatgt tcctgactgc ctgcatgagc ggcctgtacg tgattggggt
     9241 agcgaaagat atcgcccaaa gtctggcaca ccttgatgtg gtttccgcag ccaatgcagt
     9301 aacggttatt tccatcgcta acctttcagg tcgtctggtg ctgggcattc tgtctgacaa
     9361 aattgcccgt atccgtgtta tcaccattgg tcaggtgatt tcgctggtgg gtatggcggc
     9421 cctgctgttt gcaccattaa atgcagtgac gttctttgca gcgattgcct gcgtggcatt
     9481 taactttggc ggcactatta ccgtctttcc atcactggtc agtgagttct tcggcctcaa
     9541 taacctggcg aaaaactacg gtgtgattta cctcggtttc ggtatcggta gcatttttgg
     9601 ttcgattatc gcctcactgt ttggcggttt ctatgtgacc ttctacgtga ttttcgccct
     9661 gctgattctg tcattggcgc tttctacgac gattcgtcag ccagagcaga aaatgttgcg
     9721 tgaggcgcat ggctcccttt aatgctcagc cgcgagctac tgggcttcag cttccgtgtg
     9781 catcaaacgc atcaacgaaa tgtataaaca atgactcgta aaatttgggc cagttaactg
     9841 gcccttttta ctgctttttg taacgctaaa gtctcttttc aaacttgcat ttttgtaaat
     9901 ttgtgcttca tgcacactct ttccccacac tttttcgctt tgctgtggtc tacttattcg
     9961 cgcgtgtaga ttttacttat ctgactacct ccgcactttt cccctgccgg gcctgaaaag
    10021 ccgctaagca gggtgttatc acctgtttgt ccagggtttg tttgcatgag atacatcaaa
    10081 tcgattacac agcagaagct gagctttttg cttgcaatct atattggcct ttttatgaat
    10141 ggcgcggttt tttatcgccg cttcggcagc tatgcgcacg attttaccgt ctggaaaggc
    10201 gtttctgctg ttgttgaact ggccgccacc gtactggtga ccttcttttt actacgtctt
    10261 ctatcgctgt ttggccgccg cagctggcgt attctggcgt cgctggtggt gctcttttcc
    10321 gcaggtgcca gctattacat gaccttcctt aatgtggtca ttggttatgg catcatcgct
    10381 tccgtcatga ccaccgatat cgacctgtca aaagaagttg ttggtctgaa ctttattctc
    10441 tggttaatcg ccgttagtgc attgcctctt atccttatct ggaataaccg ctgtcgctac
    10501 accttgctcc gacaactgcg aaccccaggg cagcgtattc gcagcctggt ggtcgtcgta
    10561 ctggcgggta ttatggtttg ggcaccgatt cgtttgctgg atatccagca gaagaaagtg
    10621 gagagggcga ccggcgttga tttgccgagt tatggcggtg tcgtagcgaa ctcttatctg
    10681 ccatcaaact ggctttctgc gttggggctg tatgcctggg cgcgggtcga tgaatcttcc
    10741 gataataatt cattgcttaa tccggcgaag aagtttacat accaggctcc acaaaacgtt
    10801 gatgatactt atgtcgtgtt tatcatcggt gaaaccacgc gttgggacca tatgggtatt
    10861 ttcggctatg agcgtaatac cacgccgaaa ctggcccagg agaaaaatct ggcggcgttc
    10921 cgtggttact cctgtgatac cgcaaccaaa ctctcactgc gttgcatgtt tgtacgtcag
    10981 gggggcgcgg aagataatcc gcagcgcaca ttaaaagaac agaacatttt cgcggttctg
    11041 aagcagttag gattcagttc tgacctctac gctatgcaga gcgaaatgtg gttctacagc
    11101 aacacgatgg cagacaacat tgcttatcgt gagcagattg gtgcggagcc acgtaatcgt
    11161 ggcaagccgg tagatgatat gttgctggta gacgaaatgc agcaatcgct agggcgcaac
    11221 ccggatggta agcatctgat cattctgcat accaaaggtt cgcattttaa ctacacccag
    11281 cgttatccgc gtagcttcgc gcagtggaag ccggaatgta ttggtgttga tagcggctgt
    11341 accaaagcgc agatgatcaa ctcctatgac aactcggtga cctatgtgga tcactttatc
    11401 tccagcgtga ttgatcaggt tcgcgataag aaagcgattg tgttctacgc agctgaccac
    11461 ggtgagtcaa ttaatgaacg cgagcacctg cacggcacgc cgcgtgaact ggcaccgccg
    11521 gagcagttcc gcgtaccgat gatggtctgg atgtcagata aatatctgga aaatccggcc
    11581 aatgcgcagg cgtttgcgca gctgaaaaaa gaagccgaca tgaaagtgcc acgccgtcac
    11641 gtagagctgt acgacaccat catgggttgt cttggctata cttcaccgga tggtggaatt
    11701 aacgaaaaca acaactggtg tcacatcccg cagacaaaag tggcagcggc taactaagcg
    11761 gcctgctgac tttctcgccg atcaaaaggc attttgctat taagggattg acgagggcgt
    11821 atctgcgcag taagatgcgc cccgcattcg gtgattggcg cagcctggta gcgcacttcg
    11881 ttcgggacga aggggtcgga ggttcgaatc ctctatcacc gaccaaattc gaaaagcctg
    11941 ctcaacgagc aggttttttt gcatttgtag ctcatgagga tgagaacctc cgggggcagg
    12001 gaggctcgac tcgacgtagg cctgataaga cgcgccagcg tcgcatcagg caacggcacc
    12061 gcacttattg tcggatgcgg cgcgaccgcc ttattcgacc aacacaccac acattttcct
    12121 gccaactttg cgccccatat ctcactatcc attaacggat ttgtgacaga aaccattgtc
    12181 tttttttata tttgcttaaa tcttttttgc gttacttatg gctggcgctg gcattttccg
    12241 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
    12301 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
    12361 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
    12421 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
    12481 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
    12541 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
    12601 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
    12661 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
    12721 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
    12781 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
    12841 tcccgcttta taaccgcctg gttgaattta aaatcggcac caccgaagtg atcccgggcc
    12901 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
    12961 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg cgaaatgaac gccgatgacg
    13021 tggtgttctc gttcgatcgt cagaaaaacg cgcaaaaccc gtaccataaa gtttctggcg
    13081 gcagctacga atacttcgaa ggtatgggct tgccggagct gatcagcgaa gtgaaaaagg
    13141 tggacgataa caccgttcag tttgtgctga ctcgaccgga agcgccgttc ctcgctgacc
    13201 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgacgcgatg atgaaagccg
    13261 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
    13321 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
    13381 agatcgatac gctggtcttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
    13441 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatcgct cgcatgaagc
    13501 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcgtata
    13561 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca agcactaacc tacgcggtga
    13621 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
    13681 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
    13741 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
    13801 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
    13861 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    13921 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    13981 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    14041 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    14101 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcagg
    14161 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    14221 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    14281 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    14341 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    14401 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    14461 cctctgcgtg cagcgccatc cggcagcaat acttcattct ctgtccgaca agggcgggaa
    14521 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg agtcactata gagaatccgg
    14581 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    14641 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    14701 ggcgaacgtg ggatctcccc tgagcgtcac gtgcagctgc tggctgaact cggcttagat
    14761 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    14821 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    14881 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    14941 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    15001 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    15061 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    15121 ccgttaaccg gttttatgct aatcgacacc gccatctggg gtgaagacgg caactttatc
    15181 gatgccgtcg cccatatgat cttgcccgcc attgtgctgg gtactattcc gctagcggtc
    15241 attgtgcgta tgacacgctc ctcgatgctg gaagtgctgg gcgaggatta catccgcacc
    15301 gcgcgcgcca aagggctaac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    15361 atgctgccgg tagtgaccgt tatcggcctc caggtgggaa cattgctggc gggggcgatt
    15421 ctgaccgaaa ccatcttctc gtggcccggt ctggggcgct ggttgattga cgcactgcaa
    15481 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    15541 gtcaacttgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    15601 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    15661 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt cgtcgggctg
    15721 gtttacgtcg ccatcgtgct gttcatcgcg atctttgcca actggattgc gccttataac
    15781 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagt
    15841 atggcacact tgctgggcac cgatgacgtc ggtcgtgatg tgctgtcgcg gctgatgtac
    15901 ggcgcgcgtt tatcgctgct ggttggctgt ctggtggtgg tgttgtcgct gattatgggc
    15961 gttattctcg gcctgatcgc cggttacttc ggcggcctgg ttgataacat tatcatgcgc
    16021 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    16081 ttcggcccgt cgattggtaa cgccgcgctg gcgctgacct tcgttgcctt gccgcactat
    16141 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt caccgcctct
    16201 cgcgtggcag gtgccggggc gatgcgccag atgtttatta acatcttccc gaactgcctt
    16261 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggctgct
    16321 cttggtttcc tcggcatggg ggcacagccg ccaacgcctg agtggggcac catgctctcc
    16381 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttccctgg tctggcgatc
    16441 ctgttgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    16501 aaactaaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    16561 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    16621 gggcgaagtg gtcgggattg tgggtgagtc cggctccggt aagtcggtca gttcactggc
    16681 gattatgggg ctgattgatt atccgggccg cgtaatggca gaaaaactgg agtttaacgg
    16741 ccaggatttg cagcgtatct cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    16801 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    16861 tatggaagcg attaaagtgc atcagggcgg caacaaaagt acccgccgtc agcgggcgat
    16921 tgacctgctg aatcaggtcg gtattcccga tccggcttcg cgtctggatg tttacccgca
    16981 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    17041 aaaactgctg attgccgatg aaccgaccac cgcgctggac gtgaccattc aggcgcaaat
    17101 catcgaacta ctgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    17161 tgacctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggtcaggt
    17221 agtagaaacg ggcgatgcac acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    17281 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    17341 tgttgttccc ggcaagtatg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    17401 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg atgggcgtca
    17461 gtccaaatgc cattacccac ttgatgatgc cgggaggccc acactatgag tacgcaagag
    17521 gccacctcgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    17581 aaaggcatgt tcgtgccgga acgtctggtg aaggcgctgg acggcgtttc gtttaacctt
    17641 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    17701 cggttgctga cgatgatcga aacgcccacc ggcggcgagc tgtattatca ggggcaggat
    17761 ctgctcaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    17821 cagaatccct acggttcgct gaatccgcgt aagaaagttg ggcaaattct cgaagagccg
    17881 ctgcttatta acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    17941 gcaaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcacatgtt ttccggcgga
    18001 caacgtcagc gtatcgctat tgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    18061 gatgagccgg tttctgcgct ggacgtctca gtgcgtgcgc aggtgctgaa tctgatgatg
    18121 gatttgcagc aggagttggg gctttcttat gtctttatct cccacgacct gtcggttgtg
    18181 gagcacattg ctgatgaagt gatggtgatg tacctaggcc gctgcgtgga gaaaggaacg
    18241 aaggaccaaa tcttcaataa cccgcgccat ccgtacaccc aggcgctgct ctccgcgacg
    18301 ccacgcctga acccggacga tcgtcgtgaa cgcatcaaac tcaccggtga actgccaagc
    18361 ccgctgaatc ctccgccggg ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc
    18421 tgcacccagt tgcagccgca gctaaaagac tacggaggtc aactggtagc ttgttttgct
    18481 gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt
    18541 tactcaatca gcttcaacag tggcgacaag catagcaaaa tgccgtaaag cagaatcagc
    18601 caggctttaa atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga
    18661 atgaggcacg acacaatgcc atataacggg cttcccaact gaaagaacac cagcactgat
    18721 acacgaaacg aaacccaaat cgtcaacgta atgacgataa aagcgcagat cgccagagtc
    18781 agcatgcgtg agttaatttt cttggtatca ataattcggc ttaacagatt gagaataatg
    18841 cctttaatag cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag
    18901 atattcagca ccgtagaggt gatatgaatg atatgtccag ggatcacctg cgcggccagt
    18961 gccagtgccg agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag
    19021 gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg tgatataact aattcggtgg
    19081 gtacgcagcg cgagccgggt tgccagtact ttatctgctt cccgtttacg ataggcaata
    19141 ttcattgggt taagtacctg aataaatacc gcagaaaaga agcaaaatgg aatggtaagc
    19201 aggacatcgc ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag
    19261 tgcgggatca tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc
    19321 ccagaaatct taaacagtaa tcgttcacca ccagacgcaa tcgctatcag tacggcgaaa
    19381 atagcgactt tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc
    19441 aggtacgagg cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc
    19501 atcaaaaagt agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata
    19561 atatcggtgt agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc
    19621 actacccagg tgacaggata agcaatgatc gctgcggtaa taaataccca aattcccttc
    19681 aagccaattt gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat
    19741 aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct gattgtgttt cgatagtgtg
    19801 ttgtgctgca taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag
    19861 tttctcttgc gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag
    19921 attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt
    19981 ctggagtcag cccggatatg aactgcaact attgcaaata aagaaattaa gctggaaggc
    20041 atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa agcaatcgta gaatcagcgt
    20101 caagccggaa ggattccggt gaggcgcaat gttgcggggg cttaatccct ggtggcattg
    20161 gttgctggag agagaaaacc cccgcacgtt gcaggtatgt acctgacaac ataacggggg
    20221 ctaatcttga acctagacca ctcaagaata gccgcgaaac gttgtcatta caacacaggc
    20281 ggctatatga cgctcgcaga gctgggcatg gccttctggc atgatttagc ggctccggtc
    20341 attgctggca ttcttgccag tatgatcgtg aactggctga acaagcggaa gtagcgtgtc
    20401 atg
//
LOCUS       DAZSIO010000066        20288 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZSIO010000066 DAZSIO010000000
VERSION     DAZSIO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.066"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(61..819)
                     /gene="aroD"
                     /locus_tag="ACHX5T_002160"
     CDS             complement(61..819)
                     /gene="aroD"
                     /locus_tag="ACHX5T_002160"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR8467351.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="ACHX5T_002161"
     CDS             complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="ACHX5T_002161"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR8467352.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1728..3000)
                     /locus_tag="ACHX5T_002162"
                     /pseudo
     CDS             complement(1728..3000)
                     /locus_tag="ACHX5T_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3183..4388)
                     /locus_tag="ACHX5T_002163"
     CDS             complement(3183..4388)
                     /locus_tag="ACHX5T_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8467353.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4488..4844)
                     /locus_tag="ACHX5T_002164"
     CDS             complement(4488..4844)
                     /locus_tag="ACHX5T_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR8467354.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4980..5087)
                     /gene="rprA"
                     /locus_tag="ACHX5T_002165"
     ncRNA           complement(4980..5087)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHX5T_002165"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="ACHX5T_002166"
     CDS             complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="ACHX5T_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR8467355.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6774..9830
                     /locus_tag="ACHX5T_002167"
     CDS             6774..9830
                     /locus_tag="ACHX5T_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR8467356.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9827..10237
                     /gene="menI"
                     /locus_tag="ACHX5T_002168"
     CDS             9827..10237
                     /gene="menI"
                     /locus_tag="ACHX5T_002168"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR8467357.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10337..10525
                     /locus_tag="ACHX5T_002169"
     CDS             10337..10525
                     /locus_tag="ACHX5T_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR8467358.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11073..11441
                     /gene="sufA"
                     /locus_tag="ACHX5T_002170"
     CDS             11073..11441
                     /gene="sufA"
                     /locus_tag="ACHX5T_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR8467359.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11450..12937
                     /gene="sufB"
                     /locus_tag="ACHX5T_002171"
     CDS             11450..12937
                     /gene="sufB"
                     /locus_tag="ACHX5T_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR8467360.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12947..13693
                     /gene="sufC"
                     /locus_tag="ACHX5T_002172"
     CDS             12947..13693
                     /gene="sufC"
                     /locus_tag="ACHX5T_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR8467361.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13668..14939
                     /gene="sufD"
                     /locus_tag="ACHX5T_002173"
     CDS             13668..14939
                     /gene="sufD"
                     /locus_tag="ACHX5T_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR8467362.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14936..16156
                     /gene="sufS"
                     /locus_tag="ACHX5T_002174"
     CDS             14936..16156
                     /gene="sufS"
                     /locus_tag="ACHX5T_002174"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR8467363.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16169..16585
                     /gene="sufE"
                     /locus_tag="ACHX5T_002175"
     CDS             16169..16585
                     /gene="sufE"
                     /locus_tag="ACHX5T_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR8467364.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16734..17738
                     /gene="ldtE"
                     /locus_tag="ACHX5T_002176"
     CDS             16734..17738
                     /gene="ldtE"
                     /locus_tag="ACHX5T_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR8467365.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="ACHX5T_002177"
     CDS             complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="ACHX5T_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR8467366.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18348..19760)
                     /gene="pykF"
                     /locus_tag="ACHX5T_002178"
     CDS             complement(18348..19760)
                     /gene="pykF"
                     /locus_tag="ACHX5T_002178"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR8467367.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(19768..19962)
                     /locus_tag="ACHX5T_002179"
     CDS             complement(19768..19962)
                     /locus_tag="ACHX5T_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8467368.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT         5316 a         4862 c         5177 g         4933 t
ORIGIN      
        1 cggttaaaaa tacttttttt atttcggcgg gggagggtat ttccgccgaa atattattgc
       61 ttatgcctga tgtaaaatag ttaataccgt gcgcaaatca tttaccgaga tttgccctgg
      121 cgcagaggct ttttttaccg caccaaaagt tgccgccgag ccaaatactt caccaaccag
      181 acgagaaatt acgccagttt ttgccatcga catcgtgatg attggacgat cggcatactg
      241 ctcctgcatc tccagggtcg cggcaagcaa cgtcagcaca tcgctggtac tttgcggcat
      301 cagcgcaatc ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat
      361 ttcttcggct tccggcgttt tatggaagtc atggttggac atgacaactt tcacatcatg
      421 cgcgtgggcg taggcgacgg tttctttgac ctgatcatcg ccggtaaata actccagatc
      481 gatcatatca accaggccgc tgtcgatggc tgcacgattg agagcaatat aagcctcggt
      541 ggaaatcgcc tgctcgccgc cttctttggc actgcggaag gtaaacagca gcggtttttc
      601 tggcatggtt tcacggagaa tttttgccgc cgccatgaca gactccacat tggagaggtc
      661 ggcaaagtgg tccacacgcc attccagaat atcaaagtcc gcttcacgat aggcgagagc
      721 ttcggatttc acgcgggcga tatctttcgc catcagcgag acgatgattt taggtgcgcc
      781 cgcaccaatg acgagatctt ttacagttac ggttttcatt ttttaccctt tctgcacgcg
      841 gtcagcctgt caggcaccga accccatgac ctgtttaaca tattccagag ggaaatcttt
      901 gccagtccat aacgtgaact gttcagcccc ttgccacaac aacatgccgt atccatcaat
      961 cgttttgcaa ccagcttgtt gtgcctgctg caataacttc gtcatatgcg ggttatacac
     1021 gcattcagtg accagcagtc ccggatgtaa cagactgata tcattaacca atgattcatt
     1081 ctcaaggggt ttcataccta cttttgtgcc attggttaaa atgtcggcgg aagccagggc
     1141 ttcagcaaag gcttgctgat cggcgagatc ggtatccgtg acgacacaat cggtgttttc
     1201 gttaacccgc tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac ggttaaagag
     1261 tttaatttct tttaaacctt caattgcccc ctgcgcgcca atcgccgttg aggcacctcc
     1321 ggcccctaac agcaccatcg ttttgccttt gatatcaaaa ccgctctctt taatggcgcg
     1381 aatatggccc gtgccgtcgg tgttatagcc acgcagatag ccatcatcat taacgatggt
     1441 gttgatggca cccaccagtt tggctgctgg cgttaattca tcaacatatt cacacgccag
     1501 ttgtttgttc ggcatcgaca caccagttcc gcgcattttg agggctttta atccttcaat
     1561 tgctccggga aagctatcgt tatccacttc gaaggccata taggtaaatg gcaatcccgc
     1621 tttttctaag gctttattct gcatttcagg cgataaactg tggcggatag gataagccat
     1681 caatccaatc aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc
     1741 ttaattgtgt ttaaccgggt gctaaatttg cgttcgccaa accgcacatc attctcagga
     1801 ataataaaaa cgcggtaata gcggctaaaa acaataattg cggtaatcag ggccagcagc
     1861 gcgaaagtaa aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta
     1921 atcagcggaa taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg
     1981 gctttgcttt tggggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca
     2041 gctgctgaga aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg
     2101 tagataatgg cagctgttat tgttgccagc gcagagttaa atacattagc ccagatgggc
     2161 cggaccattt ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg
     2221 taataagaga tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat
     2281 ttgggcatcc acaccacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc
     2341 gatgaaacac cttccagcca gactaaaggt tttgctgttc atttgcggta attcattagc
     2401 tacgctggcg tccaccaact ggctggggaa tttgcttttc aacagcatca gcgtgatcag
     2461 tacaaacaga atacccggaa taatcagccc atagccgtac cagatattat tgagcaacat
     2521 atagctcacc agcattgggt agaacatttg cccaaatgac accatcgctt taaccagtat
     2581 gaccgccgaa ccagaggctt tcggaaagca ttccatgagc gcggggtagc cacccgtatc
     2641 cagcgctgag ttagcgatac ctacgcacac tgccagaccg taggcgagag ttaaattcgg
     2701 gcaagcggga ataccaaaga agaatagcag atacattatt actgccatta atatcaccgc
     2761 ccgacgacca aacttatcgg agatcacacc gaagaataaa atactgatca atcgccccaa
     2821 accgataccg gaaattaaat aggcaatgcc cgcgttgtca gtggaaaact tttctgacag
     2881 agatgtcata ttttgggcaa gcgtaataac actaatgccg tgcaggaaat agctgaagta
     2941 aatacaaaga acagccagga taaatggcgt gctgaaagat aatttcgtta ccgtacgtat
     3001 tgttctccga cggtttacat aattactata ctgaagatga ttttccctgg gtacatggca
     3061 taaagtttat gtttccagga atattgcatg tcatagtttg tttttgatcg cgattttcct
     3121 tagttagcgc agaaagagtt atttatccgt aagatcttgc atgacctgaa aagaatattg
     3181 cattacccac ccggagcgac attttccttt agcgagtgat gccgcgtttt tttgcggctg
     3241 cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag tatcgaacca cataatatcg
     3301 gcaatacttc tttgggacag atgagccgta atcaacggaa tagtaaaggt cgcaatactg
     3361 cccgtactgt aatagatccc tgtagcttta cctttagcgt aagggaaacg ttcagccatt
     3421 aacgtcaggc caatttgcac aacacctcca gcagaggtaa aaccaatgac aaaagcaaat
     3481 attatcacca cataaaatgt gggatgcagg cagacggtaa acagagcaat aaatgagata
     3541 aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat tacgaatgag tggagcggta
     3601 ataaatacac aaagcagcga acccacggtg tagatactga gtagtttgat cgacatagtg
     3661 tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc actggctaac cagataaaac
     3721 gttgccattg agatatagcc atataaggta taactggcta aatcgataat tgaacagcga
     3781 tgttccgtag agctggtggt tttctttatg acaggtaagc gacgacccgg atggggtggg
     3841 aacgtacaac ggtataaaaa cagagcgtta ataaacataa tgcctgcagc aatcataaag
     3901 gaccaaccga accacagttc agcccacact aacaggctaa tgattagcgg taataaaaat
     3961 tgtccgctgg aaacaaatgc tttaattaaa atattggctg tcccaggtga gcgtggaaaa
     4021 gcttccatca aactgggata agtgcctgca tcgagaaaac tgtttgccat tcccgccaga
     4081 aagccaaaaa cataagcgat aatgatgtta ttggtctgca ggatgtcaaa aaagaaggcc
     4141 atatagcagc acatcccgag catgataaaa gggcggcgac caaagcgatc ggataataat
     4201 cctgcaaaaa gcaagacact taatcgacca atgcccagcg atgagataac tatcgagaca
     4261 cccgcggcat tagtctgcca aagtgtctcc agcgaggcca tattcaggct catcaaaatg
     4321 acgcccatac catgcaccag gtaattaaaa tacaacccaa gtgcggtagg gtaataggga
     4381 tttttcatag gttctactta gcaaaggtgc tttaaaaagt gtagcgatgt tgtgtcagta
     4441 tccattcatt attgttgtaa caacgacaat tcctttctgt ggaaaaatta acaaagccgc
     4501 tccagatcgt gtgcatataa ttcagcggct accgattgat agatcttcca gtcaataaaa
     4561 ttgccatcag ggtagacttg ttgaaacgcg gttaagtcgg ggaaaaaacg catggtgttg
     4621 ttgccgatgc ggttatttat tttctctatg atggcatgaa gatgtttttt tcgttgctga
     4681 cgcatagcca acagttgctc gactacacaa tcaggaatgg cgcatttgcc attttcccac
     4741 tgacgccagc tttcactgtc accggtctgg gcaatccagg tcgcgcattc atcaatagtc
     4801 atggcaaaaa tatggcggag tgcctggagt tcataagcgt tcatcgtgta atggctgcat
     4861 gaaataaaca ataaaatata aataacatta tcagcctgct gacggcttga agagagtcac
     4921 agtatcttgt gcaacattat tggaaagagt gaggggcgag gtagcgaagc ggaaaaatgt
     4981 taaaaaaaag cccatcgtgg gagatgggaa aagactacac acagcaattc gttgtttcac
     5041 tcaggggatt tccatgctta taaatcaata tgttgattta taaccgtgag ctaatagtag
     5101 gcatacggac tttttgcgtc gatcagattc gtctcattag tttaaaaact gtaaagaatt
     5161 tgcgacaggt actaccagct gaatggcggt aagcaggaaa atatggggaa gagcaagcaa
     5221 atatcaatta gcggtgatta acaatcaggc ggcagagccg cctgatgaga aattacttat
     5281 tcggtttctc gatttcgcca agttcttcga gaatttcttc cggttggtcc gtcggtggcg
     5341 ggacttcttc cacccacgcg gcaaacagac gccaggaaac ggctaacaga accggaccaa
     5401 taaacagacc gatcatcccg aaagcaatca aaccaccaat aacgccagag aggatcagga
     5461 tcagcggtaa atcggcaccc atgcgaatta acattgggcg gatgacgtta tccagcgtgc
     5521 caaccacacc gctccacact aacaataccg ttccccaggt ggtatcgccg gtccagtaga
     5581 gccagataat cgccggaatc agtaccggca acgggccaag ctggacaagg caggagagga
     5641 tcattaacac cgttagcaac gttgcataag gtacgccgga tacggcgagg ccgataccgc
     5701 caagcactgc ctgtactaac gccgtcacca ccacacccag cgccaccgcg cggatagcct
     5761 gtgccgccag cagcacggcg gcatctccgc gaacgcctgc cagacgggtt gcaaaatggc
     5821 gaatgccttg tgccacctgt tcaccgcgcc agtacagcag ggcactgaag agaagcatca
     5881 gcgcacaatg caccataaag cgcccgatat gcgccgcctg cccaacgaac caggtggtgg
     5941 tggtgccaat ataagggcgg actttcgcca tgatcgccgt accccccata tccagcaagt
     6001 tgtgccagcc tgcatacagc ttcgcgccaa tcaccggaat ggtattaagc cacgccagat
     6061 cgggtaacgt catgtcgccg ctggaaatgg ctttaattag cgggccgctg ccgtcgacga
     6121 tactgttaac cagcaaagca atagggatga taaacaccat cactaataac agcgtcatca
     6181 ccagaacggc gagagagcgg cggccaaaca tgatcttttg caaacgtaac aataccggcc
     6241 aggtggcgat aaccaccgta ccggcccatg caaagccgag aataaagggt tgaacaatcc
     6301 acagacatgc cacaatcatg atggctaaaa acagcaccga aagcagaatt tgtgcgacat
     6361 ccctgggctg acgaacattt accataacta cttttcacct tttttgtgcg tcaaatcgtc
     6421 gacgcgacgt gaacacgcga aactcacctg cataatgatg agcaatttca gcggttttta
     6481 acaggccgat tctgccctta attctgatgg gcgtgttaga aaaaaatgtg gtacaacaac
     6541 tcaggcaaca cgcaaacgat ttactcgtcg tatttcaagt tgcatgtgct gcggctacgt
     6601 tcgttcaccc cagtcactta ctattgtaag ctcctggagt tcactcgctt gccgccttcc
     6661 tgcaactcga aatccataga gtaaataaac atcacctttc agtgcgctat tgcagccgcc
     6721 tgaaagaata gtgtatcgac ctacatcaca gaccgcagga aagggtcaat ataatgattc
     6781 cacagatttc cctggcaccc ggcgtcgttc aactggtgct taattttttg caagagctgg
     6841 agcaacaagg ttttaccggc gatgcggcga caagttatgc cgatcgtctg acaatgtcga
     6901 ccgacaacag tatttaccaa cttctccccg atgcggtggt atttccgcgt tcaaccgcag
     6961 atgtggcgct gatcgcccgt cttgccgcgc aggaacgcta ttcatcgctg atctttaccc
     7021 cccgcggcgg cggcaccggc actaacggtc aggcgctcaa ccaggggatt attgttgata
     7081 tgtcccgcca tatgaaccgc atcatcgaaa ttaaccctga agagggctgg gtgcgcgttg
     7141 aggccggggt gataaaagac caactcaatc agtacctgaa accgttcggc tacttttttg
     7201 cgccggaact ttcgaccagc aaccgggcaa cgctcggtgg gatgatcaat accgatgcat
     7261 ccggtcaggg atcgctggtc tatggcaaaa cgtcagatca cgtacttggc gtacgcgcgg
     7321 tgttgttggg gggcgatatt ctcgatacgc aacctttacc cgtcgagctg gcggaaacgc
     7381 tgggtaaatc caataccaca atcgggcgaa tttataacac ggtttatcaa cgttgccgtc
     7441 agcaacgcca gttaatcatc gacaacttcc ccaaacttaa ccgctttctt accggttacg
     7501 atctgcgtca tgtctttaac gatgagatga ccgagttcga cctgacgcgc attctgacgg
     7561 gttcagaagg gacgctggcc tttattaccg aagcgcggct ggatattacg cgcttgccta
     7621 aagtgcgccg tctggtgaat gtcaaatatg actcttttga ctctgcgctg cgtaacgcgc
     7681 cgtttatggt tgaggcgcgg gcgctttcgg tagagacggt ggactcaaaa gtgctgaatc
     7741 tggcgcggga agatattgtc tggcattccg tcagcgagtt gattaccgat atgcctgaca
     7801 aagagatgct cggactgaac attgtggaat ttgctggtga tgatgaggcg ctgattgacg
     7861 agcgagttaa tgcactctgt gtgcggcttg atgagctgat tgccagccaa caggcaggtg
     7921 tgattggctg gcaggtgtgc cgcgagctgg cgggggttga acgtatctat gcgatgcgca
     7981 aaaaagccgt tggtctgctt ggcaatgcca aaggtgccgc taagccaatt ccgtttgctg
     8041 aggatacctg cgtaccgccg gaacatctgg ccgattatat tgctgaattt cgcgcgctgc
     8101 tcgacagcca cggcttaagc tacggtatgt tcggtcacgt cgacgcaggt gtcttgcacg
     8161 tccgtccggc actggatatg tgcgatccgc aacaagagat tttgatgaag caaatctctg
     8221 atgacgtggt ggcgctgact gcgaaatacg gtggtttgtt gtggggcgag cacggcaaag
     8281 gttttcgcgc tgaatacagc ccggcgtttt tcggtgagga actttttgca gaactgcgca
     8341 aagtgaaagc ggcatttgac ccgcataacc gactcaaccc agggaagatt tgcccgccag
     8401 aaggtctcga tgcgccgatg atgaaagtgg acgcggtgaa gcgcggtaca ttcgatcggc
     8461 agatccccat tgcggtacgc cagcagtggc gcggtgcgat ggagtgtaac ggcaacggtt
     8521 tatgcttcaa ctttgatgcc cgtagtccga tgtgtccgtc gatgaagatc acccagaacc
     8581 ggattcattc accgaaaggg cgcgcaacgc tggtgcgtga atggctgcgc ttgttggcgg
     8641 atcgcggcgt tgacccactc aaactggaac aagaactgcc tgaatccggc gtcagtttgc
     8701 ggacgttaat tgcccgcacg cgcaatagct ggcatgcgaa taaaggcgaa tatgacttct
     8761 cacacgaagt caaagaggcg atgtcgggct gtctggcctg taaagcttgt tcgacccagt
     8821 gccccatcaa aattgatgtg ccggagtttc gctctcgttt tctgcagctc tatcacaccc
     8881 gttatttacg cccgcttcgc gaccacctcg ttgctacggt cgagagctac gcaccgctga
     8941 tggcacgcgc gccgaaaacc tttaacttct tcattaacca gccgctggtg cgcaaactct
     9001 cggaaaaaca tatcggcatg gttgatttgc cactgctgtc ggtcccctcg ctacaacaac
     9061 aaatggtggg gcatcgctcg gcaaacatga cgctggaaca gcttgaagct ctcaatgcag
     9121 agcagaaagc gcgcacggtg ttggtggtgc aggacccctt taccagctat tacgatgcgc
     9181 aagtggtggc ggattttgtc cgtctggttg aaaaattagg tttccagcct gtgttactgc
     9241 cattttcgcc aaatggcaaa gcccagcata tcaaaggctt tcttaatcgt tttgcgaaga
     9301 cggcgaaaaa gacggcggat ttcctcaacc gtatggctaa gcttgggatg ccaatggtgg
     9361 gcgtcgatcc ggcgctggta ctttgttatc gcgatgaata taaactggcg ctgggcgagg
     9421 aacgtggcga gtttaacgtc ttacttgcga atgaatggct ggcaagcgca cttgagtcac
     9481 atccagtggc tacagtcagc ggtgaatcat ggtatttctt tggtcactgt accgaggtta
     9541 ccgctttgcc gggcgcgcca gcacaatggg ccgcgatatt tgcccgtttt ggcgcgaaac
     9601 tggaaaatgt cagcgtgggt tgctgcggca tggcagggac ttacggacat gaagcgaaaa
     9661 accatgaaaa ttcgctcggg atctatgagt tatcctggca tcaggctatg cagcgactgc
     9721 cgcgtaaccg ctgtctggcg accggatatt cctgccgtag ccaggtaaaa cgggttgaag
     9781 gcacgggggt acgccatcct gtgcaggctt tactggagat tattaaatga tatggaaacg
     9841 gaaaatcacc ctggaagcac tgaatgctat gggtgaagga aacatggtgg ggttcctgga
     9901 tattcgcttt gaacatattg gtgatgacac ccttgaagcg acaatgccag tagactcgcg
     9961 gacaaagcag cctttcgggt tgctgcatgg aggagcatcc gtggtactgg ccgaaagtat
    10021 cggttccgtt gccggttatt tatgtaccga aggtgagcaa aaagtggttg gtctggaaat
    10081 caatgctaat cacgtccgct cggcacgaga agggcgggtg cgcggcatat gtaaaccgtt
    10141 gcatctcggt tcgcgtcacc aggtctggca gattgaaatc ttcgatgaga aagggcgttt
    10201 gtgctgttcg tcacgattga cgaccgccat tttgtgacgt tgtcaccaga aaagtgtgac
    10261 gcatattgct tttggtcatt atctgcacag taaacggtgg tatactgtgc aggtcttgtc
    10321 taaaagcgag gatgctatgt ctactcaact tgatcccacc cagctggcca ttgaattttt
    10381 acgccgtgac cagagcaatc tttcacctgc ccaatatctc aaacgtctga aacaactaga
    10441 acttgaattt gctgacctgc ttactctctc ttctgctgaa ttaaaagaag aaatctactt
    10501 cgcctggcgt ctgggcgtgc attgatcctg acttaatgcc gcaagttcgc caaatcgacc
    10561 tggattaacc tccgtccagg ttcgatgacc ttcttaatac tgcttatcta ccgcctgata
    10621 ttgttcaaaa ataatactaa tcgcagtttg tgttaaaacg gcgggttagc tttatgagat
    10681 tattcttatc gccccttcaa gagctaagcc actgagagtg ccggagataa gcgccggatg
    10741 gggtagaaac ccttaagcct gtgtcgcaca gacttaaggg ttttcttatt tctgaacgct
    10801 gcactttttt acctatctct taaatagtca ttttcaatac aactttttct ttttcatcac
    10861 agccttaatg ctccttagat aataaccatt atctaacaat gagataccta attcttagcg
    10921 tgcctgttaa cccacacatc aggggctatg cttattaaaa gcaggcaaaa agaatgtttt
    10981 acggtaaagc ccctgcgttt gctgggttga actgataatc attatcacta acatgctgtt
    11041 atacgctgaa agcgatgaag tgaggtaaat agatggacat gcattcagga acctttaacc
    11101 cacaggattt cgcctggcaa ggcttaacgc tgacacccgc agcggcgata cacatccgcg
    11161 agctggtggc aaagcagcca ggtatggtcg gcgtgcgctt aggtgtgaag caaacgggct
    11221 gcgcgggctt tggctatgtg ctcgacagtg ttagcgagcc ggacaaagac gatctgctgt
    11281 ttgaacacga cggcgcgaag ctgtttgtcc cgctgcaatc gatgccgttt attgatggca
    11341 cggaagtcga tttcgttcgt gaaggactta atcagatatt caaatttcac aaccctaaag
    11401 cccagaatga atgtggctgt ggcgaaagct ttagggtata ggcggtacta tgtctcgtaa
    11461 tactgaagca actgacgatg tcaaaacctg gaccggcggc ccgctgaact ataaagaagg
    11521 attcttcacc cagttagcca ccgatgagct ggcaaagggg ataaacgaag aggtggtgcg
    11581 cgcaatttcg gcgaagcgta atgagccgga gtggatgctg gagtttcgtc taaacgccta
    11641 tcgcgcatgg ctggagatgg aagaaccgca ctggttgaaa gcgcactacg acaagctgaa
    11701 ttatcaggat tacagctact actcagcacc atcgtgcggt aattgtgacg acacttgcgc
    11761 gtctgaacct ggcgcggtgc agcaaactgg cgcgaacgcc tttttaagta aagaggtgga
    11821 ggcggcgttt gagcagttgg gcgttcccgt gcgggaaggc aaagaggtgg cggtggatgc
    11881 cattttcgac tctgtttcgg ttgccaccac ttatcgcgaa aaactggcgg agcagggaat
    11941 tattttctgt tcctttggtg aggcgatcca cgatcacccg gaactggtgc gtaaatatct
    12001 cggcaccgtg gtgccgggga atgacaactt ctttgcagca cttaatgcgg cggtagcctc
    12061 tgacggtacg tttatttatg tgcctaaagg cgtgcgctgc ccgatggaac tttccaccta
    12121 ttttcgcatt aacgcggaaa aaaccgggca gtttgagcgc acaattctgg tggccgacga
    12181 agacagctac gtcagctaca ttgaaggctg ttccgctccg gtgcgtgaca gctatcagtt
    12241 gcacgcggca gtggtggaag tcatcatcca taaaaacgcc gaggtgaaat attccacggt
    12301 acaaaactgg ttccctggcg ataacaacac cggcggtatt ctcaacttcg tcaccaagcg
    12361 tgctttgtgc gaaggcgaaa acagcaaaat gtcatggacg caatcagaaa ccgggtcagc
    12421 gattacgtgg aaatatccca gctgcatttt gcgcggcgat aactccattg gtgagtttta
    12481 ctcagtggcg ctgaccagcg gtcatcagca agcggatacc ggcaccaaga tgatccacat
    12541 cggtaaaaac accaaatcga ccattatctc gaaagggatc tctgccggac atagtcagaa
    12601 cagttatcgc ggcttagtga aaatcatgcc gacggcaacc aatgcgcgca atttcactca
    12661 gtgcgactca atgctgattg gcgctaattg tggggcgcat accttcccgt atgtcgaatg
    12721 tcgcaataac agcgcacaac tggagcacga ggcaacgaca tcacgtattg gtgaagatca
    12781 actgttttac tgcctgcaac gcgggatcag cgaagaagac gccatctcga tgattgttaa
    12841 cggtttctgc aaagacgtgt tctcagaact gccgctggaa tttgccgttg aagcacaaaa
    12901 actcctcgcc atcagtcttg aacacagcgt cggataagga ataaacatgt taagtattaa
    12961 agatttacac gtcagcgtgg aagataaagc tatcctgcgt ggattaagcc tcgacgttcg
    13021 tcccggcgaa gttcacgcca ttatggggcc aaacggttcg ggcaaaagta ccttatcggc
    13081 aacgcttgcc gggcgcgaag attatgaagt gacgggcggc acggttgagt tcaaaggcaa
    13141 agatttgctt gcactgtcgc cggaagatcg tgcaggcgaa ggcatcttta tggccttcca
    13201 gtatccggtg gagattccag gcgtcagtaa ccagtttttc ctgcaaacgg cacttaatgc
    13261 agtgcgcagc tatcgcggcc aggaaacgct cgaccgcttt gattttcagg atttgatgga
    13321 agagaaaatc gctctcctga agatgccgga agatttatta acccgttcgg taaacgttgg
    13381 tttttccggc ggcgagaaaa agcgcaacga tattttgcaa atggcggtgc tggaaccgga
    13441 gttatgcatt cttgatgagt cggactccgg gctggatatt gacgcattaa aagtggtcgc
    13501 cgatggcgtg aactcgctgc gtgatggcaa gcgctcattc atcattgtta cgcactacca
    13561 acgcattctc gactacatca agcctgatta cgttcatgtg ctatatcagg gacgaattgt
    13621 gaaatccggc gatttcacgt tggtcaaaca actggaggag cagggttatg gctggcttac
    13681 cgaacagcag taatgcgctg caacagtggc atcacttgtt tgaagctgaa gggacaaaac
    13741 gctcaccgca agcacagcag catttacaac aattgctgcg taccggactg ccgacacgta
    13801 aacatgaaaa ctggaaatat acgccgctgg aagggctgac caatagtcag tttgtcagca
    13861 ttgcgggaga tatatcccca cagcagcgtg atgccttagc gttaacgtta gacgccgtgc
    13921 ggctggtgtt tgtcgatgga cgttacgtgc ccgcgctgag cgatgcaatt gaaggcagcg
    13981 gatatgaagt gagcattaac gacgaccgtc aggggttacc cgacgctatt caggcggaag
    14041 tgtttctgca tttgacggaa agcctggcac aaagcgtgac gcatatcgcc gtgaagcgcg
    14101 gtcaacggcc ggcaaagcca ttgctgttaa tgcatatcac ccagggcgtg gcaggtgaag
    14161 aggtgaacac tgcccattac cggcatcatc tggatctggc ggaaggtgcc gaagcaacgg
    14221 tgatcgaaca ttttgtcagc cttaatgatg ctcgtcattt taccggggca cggttcacta
    14281 tcaatgtcgc agcgaacgcc cacttgcagc atatcaagct ggcgtttgaa aacccggtca
    14341 gtcaccactt tgcccataac gatttgttgc tggctgacga tgccaccgca tttagccata
    14401 gtttcctgct gggtggcgca gtgttacgac acaacaccag tacgcaactc aatggcgaaa
    14461 acagcacgct gcggatcaat agcctggcga tgccggtgaa aaacgaggtg tgtgataccc
    14521 gtacctggct ggaacacaat aaaggttttt gtaacagccg acagttgcac aaaactatcg
    14581 tcagcgacaa aggccgcgcg gtatttaacg gtttgatcaa cgtcgcgcag cacgccatca
    14641 aaacggatgg tcagatgacc aacaataatc tgctgatggg caaactggcg gaagtggata
    14701 caaaaccgca gctggaaatc tatgcagatg atgtgaaatg cagccacggc gcgacggtgg
    14761 ggcgtattga tgatgaacag atgttctatc tgcgctcgcg cgggatcaat cagcaggatg
    14821 cccagcagat gatcatttac gcctttgctg ccgaactgac ggaagcactg cgtgatgagg
    14881 ggcttaaaca gcaggtgctg gcccgaatcg gtcaacggct gccaggaggt gcaagatgac
    14941 tttttccgtc gacaaagtgc gggccgactt tccggtgctt tcgcgtgagg taaacggttt
    15001 gccgctggct tatctcgaca gcgccgccag tgcacagaaa ccgagccagg tgattgacgc
    15061 cgaggccgag ttttatcgtc atggctacgc ggcggtgcat cgcggtattc ataccttaag
    15121 cgcccaggcg accgagaaaa tggagaacgt gcgcaagcgg gcatcgctgt ttattaatgc
    15181 ccgttcggcg gaagagctgg tgtttgtccg cggcacgacg gaagggatca atctggtcgc
    15241 caatagctgg ggcaacagca atgtgcgggc gggcgataac atcatcatca gtcagatgga
    15301 gcaccacgct aacattgtcc cctggcagat gctttgcgca cgcgttggcg cagagctgcg
    15361 tgtgatccct ctcaaccccg acggtacgct gcaactggag acgctgccta cgctgtttga
    15421 tgagaaaact cgcctgctgg caattactca tgtctccaac gtgcttggca cagaaaatcc
    15481 actggcggaa atgatcacgc ttgcgcacca gcatggcgca aaagtgctgg tggatggcgc
    15541 tcaggcggtg atgcatcatc cggtggatgt tcaggcgctg gattgcgact tttacgtgtt
    15601 ctccgggcat aaactgtatg gccccaccgg aattggcatt ctttatgtga aagaagcctt
    15661 gttgcaggag atgccgccgt gggaaggggg cggttctatg atcgccaccg tcagcctgag
    15721 tgaaggcact acctggacca aagcaccatg gcggtttgaa gccggtacac ccaataccgg
    15781 gggcatcatt ggtcttggcg cggcgctgga gtatgtttcg gcgctggggc ttaatagcat
    15841 agccgagtat gaacagaatc tgatgcatta tgcgctatca cagctggaat ctgtaccgga
    15901 tctcactctc tatggccaac aaaacaggct tggcgttatt gcttttaatc tcggtaaaca
    15961 ccacgcctat gatgttggca gttttctcga taattacggc attgctgtgc gtaccggaca
    16021 tcactgcgct atgccattaa tggcctatta caacgtccct gcgatgtgtc gggcgtcgct
    16081 ggccatgtat aacacccatg aagaagtgga gcgtctggtg accggcctgc aacgtattca
    16141 ccgtctgctg ggataacagg gaggcactat ggctttattg ccggataaag aaaaattgct
    16201 gcgtaatttt ttacgctgcg ccaactggga agagaaatat ctctacatta ttgagctggg
    16261 ccagcgtctg ccagaattac gcgccgaaga cggaagccca caaaatagca ttcagggctg
    16321 ccagagtcag gtgtggattg tcatgcgcca gaatgcgcag ggaattattg aattgcaggg
    16381 cgacagcgat gcggcgattg tgaaagggct tattgcggtc gtctttattc tctacgatca
    16441 gatgacgccg caggatattg tcaatttcga tgtgcgtccg tggtttgaaa aaatggcgct
    16501 cacccaacat ctcaccccat ctcgttcaca aggtctggaa gcgatgattc gcgcaattcg
    16561 cgccaaagcc gctgcactta gctaaactaa agggacagct ttcatccggt tggctcacat
    16621 tgccggatgg tcaatgttta cactgaccgg cacaggagtg ttttggtttc aggtgaacat
    16681 aagcaccatc cgacgtttca gcgtgagtct ccggcaaata caggaggttt acaatgaaac
    16741 gcgcgtcttt gcttacactc acgcttatcg gcgcttttag cgccatccag gctgcctggg
    16801 cggttgatta tccgctacca ccaaccggaa gccgactggt tgggcaaaat caaacgtata
    16861 cggtgcaaga aggggataaa aaccttcagg ctattgcccg acgttttgat actgcagcaa
    16921 tgttgatcct tgaagccaat aacactatcg ccccggtgcc aaaacctggt acgacgataa
    16981 ctattccttc acaactgtta ttacctgatg caccgcgtca ggggattatc gttaaccttg
    17041 cagagctgcg cctttattat tatccgccgg gagaaaatat tgtgcaggtt tatccaatag
    17101 gtattggatt gcaggggctg gaaacgccgg tgatggaaac gcgtgttggg cagaaaatcc
    17161 ctaacccaac ctggacgcct acggcaggca ttcgtcagcg ttcgctggag cgtggcatta
    17221 aattaccgcc agtcgttcct gccggaccaa ataacccgct aggacgttac gcactgcgcc
    17281 tcgcgcatgg taatggcgaa tacctcattc atggcaccag tgcgccggac agcgttggtt
    17341 tgcgcgtcag ttcagggtgt attcgcatga atgcaccgga tattaaagcc ttgttctcca
    17401 gcgtgcggac gggaacgccg gtgaaagtga tcaacgaacc ggtgaaatat tccgtggaac
    17461 ctaacgggat gcgttatgtt gaagtacatc gaccactatc agcagaagaa cagcaaaacg
    17521 ttcagacaat gccatacaca ctgccagcag gctttacgca atttaaagac aataaggctg
    17581 tagatcagaa gttagtcgat aaagcgttgt atcgtcgggc agggtatccg gtttcggtga
    17641 gcagtggagc aactcccgca gccagcaatg cgccttcagt agagtcagcg cagaatggtg
    17701 aaccagagca agggaatatg ttacgcgcga cgcagtagtg gtaaatagca gacaaaaaaa
    17761 tggcgcacaa tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag
    17821 ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg
    17881 aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt
    17941 cagaagacag ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca
    18001 ggattaccgc gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt
    18061 taatctccat gtagcgttac aagtattaca caaagttttt tatgttgaga atattttttt
    18121 gatgggaagg cacttatttt tgatcgttcg ctcaaagaag catcgaacgc tgggttttag
    18181 ataaaaaaat tgagagaaaa caggatgctt ccatcggatt catcttaaat aaagtgagat
    18241 tatatagtga aatccgattt gattttttat tgcttctggt tatcgattaa ataaaaaaag
    18301 cgcccatcag ggcgcttcga tatacaaatt aattcacaaa agcaatatta caggacgtga
    18361 acagatgcgg tgttagtagt gccgctcggt accagtgcac cagaaaccat aactacgacg
    18421 tcacctttgt gtgccagacc gctctgcaga gccagttctt tacccagacg gtagaaatca
    18481 tcagtagaag tgatctcttt aaccagctgc ggcacaacgc ctttgctcag taccaactga
    18541 tgagccgttt tttcgttggt ggtcagtgcc aggatggtgg catccgggaa gtatttacgt
    18601 actgcgcgag cagatttacc gccctgggta gcaaccacga tcagcggagc atccagtttt
    18661 tcagcagttt caacggcacc acggcatacc gcttcggtga tgcgcagttt acggttgtca
    18721 ttgttgaact cgagacggct gttcatcacg cggtcggtac gttcgcagat ggtcgccatg
    18781 atagaaaccg cttccagcgg gtatttacct tttgcggatt caccagacag catcactgcg
    18841 tcagtaccgt cgaggatggc gtttgcaacg tcaccggctt ctgcgcgagt cgggcgtggg
    18901 tttttgatca tggaatccag catctgggtc gcagtgataa cgactttacg tgcacggata
    18961 catttttcga tcatcatctt ctgggcgaag ataacttctt ctaccgggat ttctacaccc
    19021 aggtcgccac gcgcaaccat gatgccgtca gaggcttcga ggatttcgtc gaagttgttg
    19081 aggccttcct ggttttcgat tttggagatg atgtggatgt tttcgccgcc gtgcgctttc
    19141 aggtgctcac ggatttcgat aacgtcagaa cgcttacgaa taaaggaagc agcaacaaag
    19201 tctacgcctt tttcgcaacc aaagatcagg tcctgtttgt ctttttcagc cagtgctggc
    19261 agagcaatgg aaacgccagg caggttcaca cctttgtttt cgcccaggtc accgttgttc
    19321 agcactttac agataacttt gttaccttca atggcggtaa cttccatacc gatcagacca
    19381 tcgtcaacca gtacggtgtt gccaacagac aggtcagtag tgaaaccttc atacgttacc
    19441 gcaaccattt cgctgttgcc gataacagat ttatcagtgg tgaaagtaaa ggtctgacca
    19501 gctttcagag aaacatcgtt accgccttcc agtttcatgg tgcggatttc cggacctttg
    19561 gtatcaagca ggatagcggc ggttttacca gttttgctca tcacgttgcg cagattctga
    19621 atgcgctgac cgtgttctgc atagtcacca tgagagaagt tcagacgcat aacgttcatg
    19681 ccagcgtcca gcattttagc taacatctct tcagattcgg ttttcggtcc gatggtgcaa
    19741 acaattttgg tctttttcat gacagtctta gtctttaagt tgagaaggat gggagaaact
    19801 tgctttctgg gcgcactgcc ggaaagcgaa tccggtatta cgaaagtggt ggtgcctcgc
    19861 tctaaggata ggtgacatcg aaaaagcgtg cagaggaatg tgtgcttgtg tctcagccca
    19921 acgtgagtgt ctgtttttga gtcgttgagt tctacagtcc aaagtgctga aaccattcaa
    19981 gagtcaattg gcgcgcatta tacgctgaaa ttaatccaaa aggaaaacaa aaacagcgtt
    20041 tcaaaaaata tacaacttgt tagacaataa attgttatca aagatttaac gttccaggga
    20101 aaaggttacg caaaaacttc gctgtttgcg caacgtctgg tgccgaagtt gtcagtgtga
    20161 ggataagtga aagttcatcc ggtgtataaa tatatcatcg ctcaattgct acagttaaaa
    20221 taatttatcg cgttatttgt ctgattttgt ttgatttttg tttaattggt tagattgata
    20281 tatgctga
//
LOCUS       DAZSIO010000067        20114 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZSIO010000067 DAZSIO010000000
VERSION     DAZSIO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.067"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            89..865
                     /gene="exuR"
                     /locus_tag="ACHX5T_002180"
     CDS             89..865
                     /gene="exuR"
                     /locus_tag="ACHX5T_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR8467369.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1105..1188
                     /gene="yqiM"
                     /locus_tag="ACHX5T_002181"
     CDS             1105..1188
                     /gene="yqiM"
                     /locus_tag="ACHX5T_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR8467370.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1210..1872
                     /gene="yqjA"
                     /locus_tag="ACHX5T_002182"
     CDS             1210..1872
                     /gene="yqjA"
                     /locus_tag="ACHX5T_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR8467371.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1876..2259
                     /gene="mzrA"
                     /locus_tag="ACHX5T_002183"
     CDS             1876..2259
                     /gene="mzrA"
                     /locus_tag="ACHX5T_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR8467372.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2406..2760
                     /gene="yqjC"
                     /locus_tag="ACHX5T_002184"
                     /pseudo
     CDS             2406..2760
                     /gene="yqjC"
                     /locus_tag="ACHX5T_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
     gene            2798..3103
                     /locus_tag="ACHX5T_002185"
     CDS             2798..3103
                     /locus_tag="ACHX5T_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR8467373.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3106..3510
                     /locus_tag="ACHX5T_002186"
     CDS             3106..3510
                     /locus_tag="ACHX5T_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR8467374.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3500..3799
                     /locus_tag="ACHX5T_002187"
     CDS             3500..3799
                     /locus_tag="ACHX5T_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR8467375.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3985..4281
                     /locus_tag="ACHX5T_002188"
     CDS             3985..4281
                     /locus_tag="ACHX5T_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR8467376.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4351..5337
                     /gene="yqjG"
                     /locus_tag="ACHX5T_002189"
     CDS             4351..5337
                     /gene="yqjG"
                     /locus_tag="ACHX5T_002189"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFR8467377.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5630..5998
                     /locus_tag="ACHX5T_002190"
     CDS             5630..5998
                     /locus_tag="ACHX5T_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR8467378.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6205..7101)
                     /gene="yhaJ"
                     /locus_tag="ACHX5T_002191"
     CDS             complement(6205..7101)
                     /gene="yhaJ"
                     /locus_tag="ACHX5T_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFR8467379.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7206..7907
                     /locus_tag="ACHX5T_002192"
     CDS             7206..7907
                     /locus_tag="ACHX5T_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR8467380.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7930..8094
                     /gene="yhaL"
                     /locus_tag="ACHX5T_002193"
     CDS             7930..8094
                     /gene="yhaL"
                     /locus_tag="ACHX5T_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR8467381.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8306..9616)
                     /gene="cyuA"
                     /locus_tag="ACHX5T_002194"
     CDS             complement(8306..9616)
                     /gene="cyuA"
                     /locus_tag="ACHX5T_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR8467382.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9644..10975)
                     /locus_tag="ACHX5T_002195"
     CDS             complement(9644..10975)
                     /locus_tag="ACHX5T_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR8467383.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11250..12614)
                     /gene="tdcG"
                     /locus_tag="ACHX5T_002196"
     CDS             complement(11250..12614)
                     /gene="tdcG"
                     /locus_tag="ACHX5T_002196"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR8467384.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12686..13075)
                     /locus_tag="ACHX5T_002197"
     CDS             complement(12686..13075)
                     /locus_tag="ACHX5T_002197"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR8467385.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13089..15383)
                     /gene="tdcE"
                     /locus_tag="ACHX5T_002198"
     CDS             complement(13089..15383)
                     /gene="tdcE"
                     /locus_tag="ACHX5T_002198"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR8467386.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15417..16625)
                     /gene="tdcD"
                     /locus_tag="ACHX5T_002199"
     CDS             complement(15417..16625)
                     /gene="tdcD"
                     /locus_tag="ACHX5T_002199"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR8467387.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16651..17982)
                     /gene="tdcC"
                     /locus_tag="ACHX5T_002200"
     CDS             complement(16651..17982)
                     /gene="tdcC"
                     /locus_tag="ACHX5T_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR8467388.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18004..18993)
                     /gene="tdcB"
                     /locus_tag="ACHX5T_002201"
     CDS             complement(18004..18993)
                     /gene="tdcB"
                     /locus_tag="ACHX5T_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR8467389.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19092..20030)
                     /gene="tdcA"
                     /locus_tag="ACHX5T_002202"
     CDS             complement(19092..20030)
                     /gene="tdcA"
                     /locus_tag="ACHX5T_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR8467390.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         5203 a         5269 c         4812 g         4830 t
ORIGIN      
        1 caatcatcaa atttatatat gaccaatggc atacccatga ataaagtggt atgataactt
       61 cgtccgcatt ccactccggg agacgcatat ggaaatcact gaaccacgcc gtttgtatca
      121 acaacttgct gctgacctga aagagcgcat tgaacagggc gtctatctgg tgggtgataa
      181 actgcctgcc gaacgcttta ttgccgatga aaagaacgtc agccgaacgg tagttcgtga
      241 agccatcatc atgctggaag ttgaaggcta tgtggaggtg cgtaaaggtt caggtattca
      301 tgtggtttcc aaccagccgc gtcatcagca ggcggctgac aataatatgg aattcgccaa
      361 ttacggtccg tttgagttgc ttcaggctcg ccagctcatc gaaagtaata ttgccgagtt
      421 cgcggcaact caggtaacga aacaagacat catgaaactg atggccattc aggaacaggc
      481 gcgcggcgaa caatgcttcc gtgattccga gtgggattta cagttccaca ttcaggtagc
      541 cctggcgacg cagaactccg ccctggcggc catcgttgaa aaaatgtgga cccagcgtag
      601 ccataacccg tactggaaaa aactacacga acacattgat tcccgtaccg ttgataactg
      661 gtgtgatgac cacgatcaaa ttctcaaggc gttaattcgc aaagatcctc atgctgctaa
      721 gctggcaatg tggcagcatc tggaaaacac caaaatcatg ttatttaacg aaaccagcga
      781 cgatttcgag ttcaatgccg accgctatct gttcgccgaa aacccggtag tgcatctcga
      841 tacggccacc agcggcagca aatgaaattc ctgtccgaca ggcgctgcaa tgcgcctgtt
      901 tgtctcgtcg gtaagcgaaa agtataaagt gtcagcctgt gtaaatcctc tcgccaccct
      961 cccctgcaat cagcaaaatc agactccacg gacatgtaat tttgataacg aacaacgttg
     1021 acctttgtta caattagatt caatttgaat ttatgctttt gaatgctttc ttatctcacg
     1081 ttttaacagg gaatagttca ggctgtgttg atgtatcaaa cccgcagaac ataccaaaac
     1141 agcaataaca ttgcggtagt gcatctttta aaaccagcgt ggcgttaacc gattcaccag
     1201 gaataatgaa tggaactttt gacccaattg ctgcaagccc tgtgggcgca ggattttgaa
     1261 accctggcca atccatcgat gattggcatg ttgtattttg tcttgtttgt aattttgttc
     1321 cttgaaaatg gcttgcttcc ggcggccttt ttaccgggcg acagtttact ggtattggtc
     1381 ggcgtgttga ttgcgaaagg cgcgatgggt tacccgcaaa cgattctgct gctgaccgtt
     1441 gccgccagcc tcggttgctg ggtcagctat attcaggggc gatggctggg caatacccgc
     1501 accgtacaaa actggctatc tcatttaccc gcgcattatc atcaacgcgc acaccatctt
     1561 tttcataaac acggtttatc ggcgctgtta attggtcgct ttattgcatt tgtcagaaca
     1621 ctgctgccga cgattgccgg gttatcaggg ctgaataacg cgcgctttca gttcttcaac
     1681 tggatgagcg gtctgctgtg ggtattgatc ctgacaactc tgggttacat gctcggcaaa
     1741 acgccggtat ttttaaagta cgaggaccag ctgatgtcat gcctgatgct gctcccggtg
     1801 gtgctgctgg tgtttggcct ggcaggttct ctggtcgtgt tatggaaaaa gaaatatgga
     1861 aatcgggggt aagggatgca aatacctcgc atgtcgcttc gccagctagc ctggtccggc
     1921 actgttttac ttctggtcgg cacgctgttg ctggcctggt ccgcggttcg ccagcaagag
     1981 tctacgctgg cgattcgtgc cgttcatcaa ggcacaacga tgccagacgg tttttcgatc
     2041 tggcatcacc ttgacgctca tggcattcct ttcaaaagta tcacccccaa aaacgacact
     2101 ctgttaatta catttgactc cagcgaccag agcgccgccg caaaagcggt cctcgacaga
     2161 acattgcccc atggctacat cattgcgcag caggacaata acagtcaggc tatgcagtgg
     2221 ctgacccggt tacgggataa ctctcatcgc ttcggataac ttcctgttat ccgaaacata
     2281 tcactcactt tggtgatttc accgtaactg tctatgatta atgaggcggt ggttaacact
     2341 accgcttcgt tgtctggaca atcgttcctt tgtaataggt ccgaatcaca atggaaggtt
     2401 caagaatgaa ataccgcatc gctttagctg tttctctctt tgctcttagt gccggtagtt
     2461 atgccactac cctgtgtcag gaaaaggagc aaaatatcct taaggagatc agctatgccg
     2521 aaaaacacca aaaccagaat cgtattgacg gtctgaataa agccctgagt gaagtccggg
     2581 ccaactgttc agatagccag ctgcgtgccg atcatcacaa gaaaatcgca aagcagaaag
     2641 atgaggtggc ggaacgccag caagatttag ccgaggcgaa gcaaaaaggc gatgccgata
     2701 gaacggaaac tggcagaagc gcaggaagag ctgaaaaagc tggaagcgcg cgactactaa
     2761 ctcacaatag tcactactta ctcacctgga gaaaactatg tcgaaagaac acactacgga
     2821 acatctgcgt gctgagttga aatccctttc cgatacgctg gaagaggtgc ttagctcatc
     2881 tggcgagaag tcgaaagaag agttgagtaa gattcgtagc aaagcggagc aggcactgaa
     2941 acagagccgt tatcgcctgg gtgaaaccgg tgatgccatc gccaaacaaa cccgtgtcgc
     3001 ggcggcgcgt gctgatgagt atgtgcgtga aaatccgtgg accggcgtgg gaattggcgc
     3061 tgcaatcggt gtagtgctcg gcgttctgct gtcgcgtcgt taattatggc ggacactcat
     3121 cacgcgcaag ggcccggtaa aagcgttctg ggcatcgggc agcgaattgt ttccatcatg
     3181 gttgaaatgg tggaaacacg tctgcggctg gcggtggtgg agctggaaga ggaaaaagct
     3241 aatctctttc aacttttact gatgctgggc ctgacgatgc ttttcgctgc atttggtctt
     3301 atgagcctga tggtgctaat tatttgggcg gttgatccgc aatatcgcct gaatgcgatg
     3361 attgccacca ccgtggtgtt gctgctactg gcactgattg gcggtatctg gacgttacgt
     3421 aaatcgcgta aatccacctt gctgcgccat acacgccatg agctggcaaa cgatcggcaa
     3481 ctgctcgagg aggagtcccg tgagcagtaa agtcgaacgt gaacgacgta aggcgcaact
     3541 gcttagccag atccagcaac aacggctgga tatttccgcc agtagtcgtg aatggctgga
     3601 ggcaacaggc gcttacgatc gtcgctggaa tatgctgcta agtctgcgct cctgggcgct
     3661 ggttggcagt agagtgatgg cgatctggac gattcgccat cctaatatgc tggtccgctg
     3721 ggccagacgc ggttttggcg tatggagcgc ctggcgtctg gttaaaacga ccctcaagca
     3781 gcaacagctt cgcggttaac ttccctctgg ccggagcaat tccggcctta tccatcaaat
     3841 tttttgaata tttttgacag ttttccttgc taacaatcat cattcaccac gtttatgatt
     3901 ctctccatcg acagcaacga cgctaatagc gcgcccttgc acaaaaaaac aatcagcagc
     3961 ctgagtggcg cagtggagag tatgatgaaa aaattagaag atgttggtgt actggtagcg
     4021 cacattttaa tgccgattct gtttattacc gcaggctggg gaaaaatcac cgcttactct
     4081 ggtacccaac aatatatgga agcaatgggt gtcccgggtt ttatgctgct gacggcattt
     4141 ttatttcaca gcaactttgc tgaaggcgtc aactcgctga tgttcatgaa aaacctgaca
     4201 atttctggcg gattcctgct gctggcaatt accggtccgg gcgcgtatag catcggccgc
     4261 ctgctgaata aaaagtggta agcacgcgct atacttaacg ataaaaaacg aggaggaagc
     4321 tcctcgtttt tgctattgga ggagagaaaa atgggtcaac tgattgacgg cgtctggcat
     4381 gacacctggt acgataccaa atctaccggc ggtaaatttc aacgttcagc ttccgcattt
     4441 cgtaactggc tcactgccga tggcgcacct ggccccactg gcacaggcgg ttttatcgca
     4501 gagaaagatc gttatcatct ctatgtttca ctcgcctgcc cgtgggcgca ccgcacgctg
     4561 atcatgcgca aactcaaagg actggaaccg tttatttccg tttccgtagt gaacccgctg
     4621 atgctggaaa acggctggac ctttgatgac agttttccgg gagcaaccgg agatacgctc
     4681 tatcaacatg aatttctgta tcagctttat ctccacgccg atccacacta cagcggacga
     4741 gttactgttc ccgtgctgtg ggacaaaaag aaccacacca tcgtcagcaa cgaatcagca
     4801 gaaatcatcc gcatgttcaa taccgcgttt gatgcgctgg gcgcaaaagc gggtgattac
     4861 tacccaccag ccctgcaaac gaaaattgac gaacttaacg gctggattta tgacaccgtt
     4921 aacaacggcg tgtataaagc tggttttgcc accagccagc aagcctacga cgaggcggtg
     4981 gcgaaagtgt ttgaatcgct ggcgcgactg gagcagattt taggccagca tcgttacctg
     5041 accggcaacc agctaaccga agccgatatt cgcctgtgga ctacgctggt gcgttttgat
     5101 ccagtgtatg tgacccactt caagtgtgat aagcaccgca tcagcgatta cctgaatctg
     5161 tatggcttcc tgcgcgatat ctaccagatg ccggggatcg ccgaaactgt caatttcgat
     5221 cacatccgta atcattactt ccgcagccat aagaccatca accctacggg gattatttca
     5281 attggtccgt ggcaggatct cgatgaaccg catggacgag atgttcgctt cggttaaaaa
     5341 aaggcacctt acgaggtgcc tctgataatt agctaagtgc atgttgttca tgtcggatgc
     5401 ggcgtgaacg ccttatcaaa cctacaaagc gtttatattc aatatgttac aatgaccgcg
     5461 taggccagat aagcgtagcg catctggcga ttttgcgctt gccatcagtc ttagcgcgcc
     5521 ttcacggcac tttattaatt ccttgaacga atatttactg ccatttcatt cacgtctatt
     5581 cttaatttgc tgctttaaaa acaagtgatt gagcaaaatt gaggcaaaaa tggactggta
     5641 tctgaaagta ctaaaaaatt atgtcggttt ccggggtcgt gcgcggcgca aagagtactg
     5701 gatgtttatt ctggtcaaca tcatctttac gttcgtgctg gggctgctgg ataaaatgtt
     5761 aggctggcaa cgcgctggtg gcgaaggcat cctgacgata atctacggta ttctggtgtt
     5821 tttaccgtgg tgggcggttc agttccgccg cctgcacgac accgaccgct cggcgtggtg
     5881 ggcactgcta ttcttaatcc cgtttatcgg ctggcttatt atcatcgtct ttaactgcca
     5941 ggcgggtacg ccaggcgaaa accgctttgg accagatcca aaactggaac aagaataatg
     6001 attaagcacc ttgtaaaagg tgcttcaaat tgttgacaaa gtgcgctttg tttatgccgg
     6061 atgcggcgta aacgccttat ccagcctaca aattgccaaa attcaatata ttgcagtgac
     6121 tgcgtaggcc tgataagcgt agcgcatcag gcagttttgc gtttgtcagc agtcagaagc
     6181 accttgtgaa aggtgcttaa tagattattt tccagcgaac aactttggaa tttcccgcag
     6241 acaccaggat ttcgcttccc ccatactgtc acgacgccag gccataataa tatcgatctc
     6301 gctggtcgat tccgggctga cgacacgcaa ccgcccttcc gcaatatctt tttcgaccat
     6361 cggatacggc atcgtcgcca cgccaagccc cgccagtaat gcctgacgtt tatcttcaat
     6421 cgtgctcacc gttaagcgcg gctgtttgtc cagcagctgt acggtcaaca ccgggcgctc
     6481 acgagcggta tccgctaccg caattccacg atatttcaca cgcgtcactt cagataacgg
     6541 ttccggctcc tgatgaatcg ggtgatcagg cgcggcaacg tagacgttca ttaacgtata
     6601 gagcttgcgc gagttgatct ccgacgagga acgaaaatgc atatccggcg cgataacgat
     6661 atccgcccgc ccctgctcca gccgttccca cgcccccgcc agcacttctg tgatgattgc
     6721 cagttgggta ttggcttttg ccgccagttt gtcgattaac gggaaaaagg caggtgtcgg
     6781 taccagcgct tcggtcacaa tggtgagatg cgtttcccaa ccgcgcgcga gagcttccgc
     6841 atcagtagtc agtttatctg cggcttccag caaaacgcgc cctcgctcca gcaacatcct
     6901 cccgacattg gtgaatttgg tgcgatggcc cgagcggtca aacagcacca catccagctc
     6961 ttcttccagt ttttgcatgg tgtagctaag tgcggaaggc acgcgtccca gctcatccgc
     7021 tgccgccgca aaactgcccc ggcgatcgat cgcatccata acccgtagtg cttccagcgt
     7081 taatgccctt tctttggcca tttcgttctc attcaggaaa tttgaacata ccgggcagaa
     7141 tatctggcta acaatgtagc gtccagcccc ttaccataaa aggaagtaaa gagaggtcaa
     7201 gaattatgat tactacccga actgccaggc agtgtggaca agcagactac ggatggttgc
     7261 aggcccggta tactttttcc tttggacact acttcgaccc gaaattgtta ggctatgcct
     7321 ccctgcgtgt gcttaaccag gaagtgctgg ccccaggtgc cgcctttcag ccgcgaacct
     7381 atcccaaagt cgatatttta aatgtgattc tggatggaga agcagagtat cgcgacagcg
     7441 aaggcaatca tgttcaggcc agcgccggag aggcgttgct gctctctacc cagccgggtg
     7501 tcagctatag cgaacacaat ctcagcaaag acaaaccgtt aacgcgaatg cagctttggc
     7561 tggatacctg cccgcagcga gagaatccgc tgattcaaaa gctggcgctt aatatggaca
     7621 agcagcaatt aatcgcctcg ccagagggtg cgatgggaag cctgcaatta cgccaacaag
     7681 tgtggctgca ccatatcgtg ctcgacaaag gcgaaagtgc gaatttccag ttgcatgggc
     7741 cacgcgcgta tttgcaatcg attcacggga aatttcatgc gcttacgcat catgaagaga
     7801 aagcggcgct aacctgcggt gatggggcgt ttattcgtga cgaggctaac attacgctgg
     7861 ttgccgattc cccactgcgc gctttgctga tagatttgcc tgtctagttg tttttacagg
     7921 gagatgatga tgagtaaaaa atcgaccaaa aagcgccagc cagcgaagcc cgtggtggcg
     7981 aaagaacctg ctcgcaccgc caaaaatttt ggctatgaag agatgttgag cgagctggaa
     8041 gctatcgtcg cggatgctga aacgcgttta gccgaggatg aagctaccgc gtaaagacag
     8101 gttgccggat gcggcgtaaa cgccttatcc ggcctacaaa acacaacaac atcaacaaat
     8161 taaacacccc gtaggccgga taagatgcgc cagcatcgca tccggcatca ggtacaaaac
     8221 acaacaacat caacaagttg agcaccccgt aggccggata aggtgcgcca gcatcgcatc
     8281 cggcacaata ctcaaaacct ggcgtttatc tggccttgct cgccataatc tcgataatct
     8341 gccgatccgt ttgctgcatc gaatggcttg ctaacgcaca caggttggca atcgactgct
     8401 caacatcatg cgccacgatc ccttcattgc cggtcacggc ggtatcatcc agcgccatta
     8461 acaccgcttt ccacgcagcc gaagcactgg tcgaaacctt catcgcgcag ctgttcgacg
     8521 caccatcgca aatcatgccg ctgacatcgc cgatcatact gctgatcgcc atcgagatag
     8581 tttcataacg cccatccacc agccatgcca tcccggcggc ggcccccatt gctgcggtcg
     8641 ttgcggcaca aagtgcagac aaacgcggta actggttatg gatgtaaatt gcgctcaaat
     8701 gagaaagcat cagcgcacgc gccagtcgtt catcatccgc tccgaagtgt tctgctacaa
     8761 ccaccacagg cattattgca gtgatcccct ggttacccga gccggagtta ctcatagccg
     8821 gaagcgtagc gccgcccata cgcgcatcgg atgccgcgct ggtacgaatc acaatggatg
     8881 aagagagatc tttcgccagc aaaccgcgct cgcactgttt ttccagcgtc gcgccaatat
     8941 gcagccccca cttaccgctc aaaccttcct gcgataacgc acaatttagc ttcgcggaat
     9001 cgagaataaa gcggatcgcc gcaaatggga cttcattgac gaacttcagg atctcagcca
     9061 gcgtcgttct ggaaagcacc gtcagcggcg actcttgctc gccctctgcc acacacgcct
     9121 ggtgggtaaa caccacacca ttgtgcgtct caatatgcac aatgttggta tgcccgccga
     9181 cgatggtgac acacgcccac ttctcaccgt tccagacttt ggcgcgtgag aagaggattt
     9241 cattgcaagg ttcctggatc ttaacagaga ctttccccgc cgccagcagt gctttggcat
     9301 cggtaattgc ctgcgcagtt gcgtctttca gcacttccag cccggcgttg gcatttccac
     9361 ctaacgcccc cagcgccgcc gcaatcggca gccccaccat tcccgtgccg ggaacggtga
     9421 cgcccagacc gttcttcatc agatttggcg aaacccaggc ttctacacgt tcaaccggac
     9481 cttccagttc tgccgcagca accgccgccg ccagcgccag tgaaatcggt tcagtacatc
     9541 ccagcgccgg ttttacttcc tcctgaacgg cgaggatgta acgctgccat aacggattta
     9601 aagtcgaatc aaacatatta aaaaccttaa aatttcaggt aaatcaagaa aatgccagga
     9661 acagagaaac acaaagcaac aaaccagtga cgataatcag gtacagagac atccctttgt
     9721 atttgtgcaa tgccggtact ttgtaaacca gccacgccgg gatcaggcac cctaccatgc
     9781 cgaaaatcgg gctacagata gaggtgaagc tcaacaccgg tgcgttcagt acgatggcgc
     9841 tccaggccag caaaatggcg aaaatcatga tgccgcgctg aacaagattt tcgttaatct
     9901 tctcggcagg catcttgcga cgcaggatgt tcattacgat cccttgcgtt gcttcgcgaa
     9961 agcctaaata gacgccaaag aatgcagtca tgacagcaaa gatattgaga atgacgctga
    10021 ccactttcac ccacgctgcg ccgtcaccgc taataaactg tgcggcaatc gccagcgcgg
    10081 aaatattctg ctcgtaggct tttaccgctt cgtcatgtcc catcgccagc gtgaacgaca
    10141 cggcgtagaa aaagacggtg acaaacaaaa tgccaaacgc gatattcatt gcccgcaatg
    10201 ctttatgccg cgccacttca attgattttt cccgcgagcg ataagagatc accatcgggc
    10261 ttaacgtctg gataaacaga atcgacgtca gggtaaacgg cagcgtaata atggcgtttt
    10321 tcaccagcag ccccagcggc ggtagcgaac cgacgttata cagatgccac attcccacca
    10381 tcgacacgcc cagcgcagcg actaccagca gcttggtcag caccatgtcg gtcgaaattt
    10441 tgaataacaa tttctcgccg cgtgaagaga tcgccaccag aatgcaaatc agtaccagac
    10501 cataaaaggg actgtctgac agcaaccctt ccgtcacgcc gaaggtatgc agataggaag
    10561 cactatcgtt ggtgatggcg gtggaataga cgaacatcca aatcaccagc attacgaaat
    10621 agagcgcacc taacaggatg ccccagtttt tacctaaata accgctaatg acactcgggt
    10681 aatctttaca ttctggtgat tctgccagcg tattaataaa caaccgctga aacagataca
    10741 ttgccgggta accaatcacc gatgagagca aaaataccca caatcccatc aaaccgacct
    10801 gcaccgggag aaaaacaatc cccgcgccaa tcgccatccc gatactcata atcacccagc
    10861 cggtgtcagt actgtcgaat ttgatcgctt ctcgccactc gctctcactc attccagcac
    10921 gacccgccgg ggtcgaagcg tctgcaatga cgcctttatt cgatgcaatt tccataattt
    10981 ctcgctcaat attttgtagg gcttattatt tttttccgag ccgcatcaag gcgatatgcg
    11041 gtctacgttt ttgcaggcga gttattagaa taaagaaatg atacgcgcga ggtcagagaa
    11101 aatcttcaca atcaagactt taaaataacg gagatggata agaattttct actaaattaa
    11161 tcacaagaga atattcattc tctatttgcg gcagatcaca aaaaaaaggt gcacatttgt
    11221 gcacccaagg atgaaagctg acagcaatgt cagccgcaga ccactttaat ggccagtcct
    11281 ccgcgtgatg tttcgcggta tttatcgttc atatctttgc cggtttcata catcgtctcg
    11341 atcactttat cgagtgaaac acgcggtgcc gaggtgcggc gcatcgccat ccgcgcggcg
    11401 tttactgctt tcacggcatt aatggcatta cgttcaatgc acgggatttg tacctgtccg
    11461 gcaaccggat cgcaggtcag cccaaggtta tgctccatcg cgatttccgc cgcattgcat
    11521 acctgcgccg gactgccgcc cagtagttca gttaaccctg ccgctgccat tgaacaggcc
    11581 acgccaatct ccccctgaca gccgacctcc gcgccagaga tggaggcgtt cattttatac
    11641 agcgcgccaa tagcccccgc tgccagaaaa tagcgggcaa ttgaccgctc gtttaccgga
    11701 cgacggaact tatcgtaata agccagcact gccggaataa tgccgcacgc accgttagtc
    11761 ggtgccgtta ccacgcgccc gccagctgcg ttttcttcac taaccgccag cgcgtacatg
    11821 ttgatccagt cgatgacatt catcggatcg ttagagatgt tatcgctaga aaccagctga
    11881 cgacgcagcg ctacggcacg acacggcaca ttgagcggac caggcagcac gccttcagtg
    11941 ttcatcccgc gttcaatacc gtcatgcatc acttgccaga tacgggcaaa ccctgcgtca
    12001 atttccgctt tgctgcgcag cgccagctcg ttgtgcatca tcaggccaga tatagacagg
    12061 ccgttgtaat cacacatttt cagcagttca cctgctgagt ggaaatcgta aggtacggac
    12121 gtttcgacat cgtgcgacag gccgaagtgt tcttcttcga caataaaccc gccgccgaca
    12181 gagtaatagg ttttacttaa tagcgcttcc tgtcccttcc aggcagtgat ccgcattccg
    12241 ttctcatggc gaggcaacat ttcgggatgg aagataatgt tctttgctac aggaaaatca
    12301 acaatatgcg caccagatgc cactggcagc cgcccgctgc gcgttactaa ctctataaat
    12361 gcagggatct catcaatgac aacatcctgc ggactgtttc ctgccagtcc catgataatg
    12421 gcgacatccg tggcatggcc tttgcccgtc agtgacaacg acccgtacag atcgaccaca
    12481 atatggctcg tcgcggttaa taagccgcta ctttccagcc gatcaataaa actttttccg
    12541 gcattcattg gccccacggt atgcgaactg gagggaccaa tcccaatttt gaaaatatcg
    12601 aatgcactaa tcatatccac accctcggat tgccgttcag tgaagtggag cggaacgacc
    12661 ttgcgaccgt cccgctcacg aggctttacg cactacgtac tgcgatagct tcaatttcca
    12721 gcttcacatc tttcggcaaa cgcgcaacct gcacacagct ccgggtcggg taggtcgcct
    12781 gatgctcatc gaagaactgc ttatagactt cattgatggt ggcaaaatca ttcagatcgg
    12841 tgatgaacac ggtcatcttg atgatatcgc ccacgctcag cccggcagca accacgatcg
    12901 ctttgacgtt ttcgaggctt aaacgcgcct gatcttgcac atcagccggg atctcaccgg
    12961 tctgcgggca aaccggaatt tgcccggagg tgaagaccat gctgcctaaa tcaacgccct
    13021 gaacataagg gccgattgcg cctggggcac gttgcgtttc gataatcttt ttcatacatc
    13081 ctccggcgtc agagcgcctg ggtaaaagta cgtgaaataa catcctgttg ctgttcacgg
    13141 gtcagtgcgt tgaagcgcac ggcgtagcca gagacacgga ttgtcaggtt aggatatttt
    13201 tccgggtgct cgatggcatc cagcagcatt tcccgattca ttacgttgac gttgaggtgt
    13261 tgaccgcctt cgacatccgc ttcgtggtgg aaatacccat ccagcaggcc gacaaggttg
    13321 gttttacgta ctggatcttc tttgcccagc gccgcaggaa cgattgagaa ggtgtacgag
    13381 atcccatctt tggcgtaggt gaacggcagt ttcgccaccg acgtcaatga ggccacggca
    13441 cctttgcggt cacgaccatg catcgggtta gcgcccggcg cgaacggtgt tccggcgcga
    13501 cgaccgtccg gcgtattacc ggttttctgg ccgtacacca cgttagaagt gatagtcaga
    13561 atcgactggg tagggacggc gttgcgatag gttggcagcg ctttaatttt cttcataaag
    13621 cgttcaacca ggtcgcaggc aatgctgtct acgcgctcgt cgttgttgcc gtactgcgga
    13681 tattcaccgt cgatttcaaa gtccaccgcc aggccgtttt cgtcacggat tggtttcacg
    13741 cgggcatatt tgatggcaga cagggagtcc gtcgccaccg acaggcccgc gatgccgcat
    13801 gccatagtgc gatagacatc acgatcgtgc agcgccatca gcgaagcttc gtagctgtac
    13861 ttgtcgtgca tgtagtgaat gatattcagc gcgctgatgt actgcaccgc cagccagtcc
    13921 atgaagtgat cgaggctgtc catcactttg tcgtaatcca gcacgtcgtc catcagcggt
    13981 gctgttttcg gcccgacctg aatcttcagc ttctcgtcca ccccgccgtt aattgcgtag
    14041 agcagcgttt tcgccaggtt agcgcgtgca ccaaagaact gcatttgctt accaatcacc
    14101 attgggctga cgcagcaggc aatcgcgtaa tcgtcgctgt tgaagtcagt acgcatcaga
    14161 tcgtcatttt catactgcaa ggaagaggtg acgatcgaca cctgcgcggc atattttttg
    14221 aaggcaatcg gtaattcttc cgaccaaaga atggtcaggt taggttccgg tgccggcccc
    14281 atagtgtgca gggtgtgcaa atagcggaag gagtttttgg tcaccagcgt acgaccgtcc
    14341 agccccatcc cgccgatcac ttccgtcgcc cagattgggt cgccggagaa cagcgaatca
    14401 aattccggtg tacgcaggaa gcgtaccata cggatcttca tgatgaagtg atcgatcagt
    14461 tcctgtgcct gctgctcatt gaggacgcca gctttaaagt cgcgctcaat gtagatatcc
    14521 aggaacgatg ccgtgcgccc cagcgacatc gcgccgccat tttgcgattt cactgccgcc
    14581 agataagcga agtagagcca ctgcaccgct tcctgtgcat tctgcgccgg gcgagagata
    14641 tcaaagccat atttcgccgc catttcctga atctgcaaca gcgcatgacg atgctctgcc
    14701 agctcctcac gcagacggat ggtggcttcc agatcctcgc ctttttccag acgagactgg
    14761 agatcggcaa attgcagttc gcgttcacgc accagataac tgatgccata cagcgctacg
    14821 cggcgatagt caccgataat gcgcccacga ccatagccat ctggtaaacc ggtcagcacg
    14881 ccagatttac ggcagcgcag catatccggt gagtaaacat caaatacgcc ctggttatgg
    14941 gttttacgca gatcggtaaa cagatattca aattcactgt ccatttcacg gccataggcg
    15001 tggaatgaac ttttaatcat attaatgcca ccgaacgggt gtagcgcacg tttcaacggc
    15061 gcatccgttt gcaggccaac aattttttcc agcggctggt taatatatcc cgcatcatga
    15121 gcggtaattg tggtggcaat attggtatcg aaatcaaccg gcgcgtgggt tgcgttttcg
    15181 atacggatgc cttccattac cttttcccac aattccgtgg tggcaggcgt cgcttcggcg
    15241 agaaaagatt catcgccttc atacggtgta tagttatgtt gaataaaatc gcggacatta
    15301 atttcgcttt tccagtccgt acctttaaag ccaagccatg cgtcagcgta cagcttatcg
    15361 ctggtatcaa tatctacctt catgaaaaat aatctctcta caatacttca actaaattat
    15421 gcaaattctg cgggcgcgtt aactttacct aaatgaatgg catccaaagc aatcattttt
    15481 tcttcgttag tcggaataac gacgcaaatg acatgcgcat tttcactgga aacaattcgc
    15541 tcgccaaaag agttagaacg attattcatt tctgtatcaa tctctacgcc taatacagcc
    15601 aaatgttcca tgaccagacg acgaattaaa cttgaattct ctcctattcc gccggtgaat
    15661 ataattccat ccagccgacg taatgaagct gcatgtccgg caatatgacg ggcaattcgg
    15721 tgaacaaagg ttttaattgc cagttgcgcg cgttcgtgac cttcatgcca ggctttttcc
    15781 agaacacgta aatccgaaga aagaccggaa atacctaata atcccgactc tttattcact
    15841 acgcgttcca ggtcacccag gctctggttg gtttggctgg cgacccagga catcgcacca
    15901 aagtcgacat cgccactgcg ggtacccatc atcaagcctt ccagcggcgt cattcccatt
    15961 gaggtgtcaa cactctgacc gttgcgaact gcgcagattg acgcgccatt gccaagatgc
    16021 gccacaacca ggccggagtc atcttccgcc agattcagca gcgaatgtgc gcgctgggaa
    16081 acatagcggt gcgacgtgcc gtggaaacca taacggcgta cacctaactc ttcataatat
    16141 ttccacggca ggccgtataa ataagcttcc ggagccatcg tctggtggaa actggtatca
    16201 aataccgcca cctgagttac gcccggaaat aattgctgcg ccgattcaat accacttaaa
    16261 tttgcgtaat tatgcagggg tgccagtgga gaaacgcgac ggatattatc aatgacttca
    16321 tcggtaataa tggcggactc ggtaaaaata ctgccgccgt gagcgatgcg gtggccaatt
    16381 aaggccacgc tgtcatttaa attccgtttt tccagttcaa atgcaattgc cttcaatgca
    16441 ccttcgtagc tgtggtgagc cagcggtgct ggctctcccc catttacgga taagaatgca
    16501 ttttccgagt taataccgtc ggcaatacct gacattaata cttcacaatc gctggcatcg
    16561 agtacggaaa acttaatcga agacgaacca cagttaataa ccaaaacaac cggaaattca
    16621 ttcatctctt ttctcatcct gagttacgga ttaaaacagt ttgtatacga tgttcaggat
    16681 ggtcagcaga ccaatcacgg taacaaacac gttatccaga cgaccacggt atttcgccag
    16741 agacggcgct ttacggatag catacatcgg caacaggcac agcagggatg cgataatcgg
    16801 tgcgcccatg gcttcaatca ggtcgaggat gttcgggttg gcgtaggcaa caacccaggt
    16861 ggagcccatg atgaagatca tgctgagagt attcagttta cccagcgaca ctttggtttt
    16921 gtcaccttta taaccgaact tcagaatcag accattcaag ccttccagcg tccccagata
    16981 gtgaccgaag aaagatttga agatagccac gagtgcgatg atggaagccg catattccag
    17041 tgtaatcgcg aacgttgttt tcgtaccggt catggacgca aagtggttag ccagataaga
    17101 aagcactgga atattctgcg ctttggcttc cgccatgttg gccggagaca gagtaaacag
    17161 gcagctaaag gcaaagaaca tcaccactgc aaccatcagc atgctggcac gagaaatgat
    17221 ttgggaacat ttacgttcgg tgaagtcgcg accgaagtct ttctcatact cttcacgttt
    17281 agaaaccacg aaggaagaga cgattggcga gaagttaaag gagaaaacca tgatggaaat
    17341 ccccagccag acagtgatca ggataccgtc atgaccggtt aacgacagcg aaccgaggtc
    17401 aacctggtcg ataactgcag agttccagta agggatcagc gacagagaaa tcagcaccag
    17461 gctggcgata aacggccata ccaggtagct catcacttta accatcagat ccttaccaaa
    17521 ccagatgacg aaagccatca gcagcaacag gaacagcgcc acaaagccgc gattcagcgg
    17581 tgcaaatccg agctggtttt cccagaacgt cataaaggta ttggtaatag taacgccata
    17641 aatccacagc agtgggcaaa tcgcgaagaa gtacaggaac gtgataacca cgccgccagt
    17701 tttaccaaaa tgctcttcca ccgtttccgt aatgttgccg gaagggttag agccagaaag
    17761 acacagacgc gccagcgccc ggtggcaata aaatgcgatg gggtatgcca ataccaacat
    17821 cagaagaatc gggatcagtc cgccaaaacc tgcgcggata gggaagaaca gcaccccggc
    17881 gccgattgcc gtaccaaaca agcctaacgt ccatgtggta tctgatttac gccaggacga
    17941 ttgttttgtc tggctggata caatgctatc tgaagtactc atatcctatc ctcaacgaat
    18001 taattaagcg tcaacgaaac cggtgatttg agagacgcga gaaagatcga tattgccgcc
    18061 ggaaataata ctgacggttt ttctattttg aatatattgg tctaatttac cgcttaataa
    18121 tgcagcacat gccagagcgc ctgcgccttc ggtgacgact ttattgcgct gaattaaggc
    18181 aatcatactg tttctgattt cgtcttcgct gaccagcacg atgtcatcga ctaattcacg
    18241 aacgatttcg taagttaaat tacccgggcg ggagacatca caaccatccg ccagggtgcc
    18301 ggtagttcgg tgcgtggtta tttctccgga gtggaaagaa gccgccatgc cgtgaacgtt
    18361 ttctgactgt acgccaataa cacgaatggt cgggttaata gatttaattg ccaccgcaat
    18421 accagcaatt aaaccaccac caccaattgg cacaatcacg ttatcgacat catagagatc
    18481 ttccataatt tccagaccaa tcgttccctg gccagcaatc actttcggat catcgtaagg
    18541 tgggataaaa atacgacctt ccatttcgac aatttcgctc actttagcga tagtgtcgtt
    18601 gaagttatca ccatgcagaa cgacttctgc ggagtagtcg cacgttgccg ctactttgga
    18661 ttttggcgca ccttttggca tcaccacttt accgtcgata cccagcatcg cgcaggagag
    18721 cgaaacccct tgcgcatggt tgcccgcaga acaggccacc acgcctttgc gtttttccgc
    18781 atcggtcagt gaacttaatt tattaaatgc gccacgaatt ttaaatgaac ccgtacgctg
    18841 catattttca aacttcagga atatttcacc tttgcaacgt tcactaaaat agttggagcg
    18901 aggcatgcct gttttataaa ttcgcccagc cagtcgttgt ttcgcttcaa taatatcatc
    18961 aatagcaacc ggcagatcgt atgtaatatg cattataaaa cctcttcgcc tgaattaaat
    19021 atgtaggtaa ccgtaaccga cacctgcaaa acagacaggt ggattattta tgtttaaata
    19081 aaaacaaaca actaaccaac ttctattaat tgccttcgtc tacacccatt ataagatgaa
    19141 tactctttgg ctaattccac caaaaccgat gctgcttttt taatacgata atttttcgac
    19201 cataccgcgg catattgtgc cacaggtaat gtttcttcaa ccggaatagt aataaattga
    19261 ttagaaccaa aaggtgacgt catatcacaa ggaattacag ttaagaaatc agcattgaga
    19321 acaagattat aaattgtcac gactgagtcg gttttaacga tgttttcaat actgatgcca
    19381 tttctttgta acgtagtaag cagttcgctg tagtacccca tattagtttg tggcaacacc
    19441 cactgttcgt tcttcaacga ctccagcgtg gtggtgccgg tgcatgttcg ggacttactg
    19501 gctaccagca caaactcgga ctcgaacagc ggctcaacat gtaaatcctg aagcttcatt
    19561 tctgcactta acgtaccaat cgcaaaatcc agtcgaccgt cgcggattgc cggtaagaac
    19621 gaagacagtt gcgcttcata catagaaacc tgcgctttcg ggaacacctc tttgaacttg
    19681 ttgatcatcc ctgacataaa agtaaaacca atcaatgaag gaaaaccaaa tgagacttcc
    19741 accaccgctt cagaagacat accgcttatc tcattaacca tatttttcat ttcacgggta
    19801 atggattcgg aacgggagag taacaattga ccggcaggtg ttaatgttac accggtattt
    19861 ttccgcacca ctaattccac accaaaataa tcttcaatat cgttaatgat tttactgacg
    19921 gccggttgag ttaaccctaa ttcttttgca gccgagccga tagagccact tctaatgact
    19981 tcctgaaaga ctaccaggtg ctgcgttttc ggaagaagaa tagtgctcat aacgacctac
    20041 gttaattacc tcattgacgg catgaagtgt atcaaaatga aataaacagg atatgtgcga
    20101 ccactcacaa atta
//
LOCUS       DAZSIO010000068        20020 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZSIO010000068 DAZSIO010000000
VERSION     DAZSIO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20020
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.068"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            241..1335
                     /gene="gcvT"
                     /locus_tag="ACHX5T_002203"
     CDS             241..1335
                     /gene="gcvT"
                     /locus_tag="ACHX5T_002203"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR8467391.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1359..1748
                     /gene="gcvH"
                     /locus_tag="ACHX5T_002204"
     CDS             1359..1748
                     /gene="gcvH"
                     /locus_tag="ACHX5T_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR8467392.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1867..4740
                     /gene="gcvP"
                     /locus_tag="ACHX5T_002205"
     CDS             1867..4740
                     /gene="gcvP"
                     /locus_tag="ACHX5T_002205"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR8467393.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            5007..5750
                     /locus_tag="ACHX5T_002206"
     CDS             5007..5750
                     /locus_tag="ACHX5T_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR8467394.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(5807..7240)
                     /gene="bglA"
                     /locus_tag="ACHX5T_002207"
     CDS             complement(5807..7240)
                     /gene="bglA"
                     /locus_tag="ACHX5T_002207"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR8467395.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            7285..7595
                     /gene="yqfB"
                     /locus_tag="ACHX5T_002208"
                     /pseudo
     CDS             7285..7595
                     /gene="yqfB"
                     /locus_tag="ACHX5T_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
     gene            7759..8418
                     /locus_tag="ACHX5T_002209"
     CDS             7759..8418
                     /locus_tag="ACHX5T_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR8467396.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(8614..9594)
                     /gene="ygfZ"
                     /locus_tag="ACHX5T_002210"
     CDS             complement(8614..9594)
                     /gene="ygfZ"
                     /locus_tag="ACHX5T_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR8467397.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            9837..10103
                     /gene="sdhE"
                     /locus_tag="ACHX5T_002211"
     CDS             9837..10103
                     /gene="sdhE"
                     /locus_tag="ACHX5T_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR8467398.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            10084..10491
                     /locus_tag="ACHX5T_002212"
     CDS             10084..10491
                     /locus_tag="ACHX5T_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR8467399.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(10531..11052)
                     /gene="fldB"
                     /locus_tag="ACHX5T_002213"
     CDS             complement(10531..11052)
                     /gene="fldB"
                     /locus_tag="ACHX5T_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR8467400.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            11164..12060
                     /gene="xerD"
                     /locus_tag="ACHX5T_002214"
     CDS             11164..12060
                     /gene="xerD"
                     /locus_tag="ACHX5T_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR8467401.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            12085..12795
                     /gene="dsbC"
                     /locus_tag="ACHX5T_002215"
     CDS             12085..12795
                     /gene="dsbC"
                     /locus_tag="ACHX5T_002215"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR8467402.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            12801..14534
                     /gene="recJ"
                     /locus_tag="ACHX5T_002216"
     CDS             12801..14534
                     /gene="recJ"
                     /locus_tag="ACHX5T_002216"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR8467403.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            14625..15723
                     /gene="prfB"
                     /locus_tag="ACHX5T_002217"
     CDS             join(14625..14699,14701..15723)
                     /gene="prfB"
                     /locus_tag="ACHX5T_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR8467404.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            15733..17250
                     /gene="lysS"
                     /locus_tag="ACHX5T_002218"
     CDS             15733..17250
                     /gene="lysS"
                     /locus_tag="ACHX5T_002218"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR8467405.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(17293..17841)
                     /gene="idi"
                     /locus_tag="ACHX5T_002219"
     CDS             complement(17293..17841)
                     /gene="idi"
                     /locus_tag="ACHX5T_002219"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR8467406.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            17896..17967
                     /locus_tag="ACHX5T_002220"
     CDS             17896..17967
                     /locus_tag="ACHX5T_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8467407.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(17964..18089)
                     /gene="yqfG"
                     /locus_tag="ACHX5T_002221"
     CDS             complement(17964..18089)
                     /gene="yqfG"
                     /locus_tag="ACHX5T_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR8467408.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(18091..19539)
                     /gene="uacT"
                     /locus_tag="ACHX5T_002222"
     CDS             complement(18091..19539)
                     /gene="uacT"
                     /locus_tag="ACHX5T_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFR8467409.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         4935 a         5000 c         5374 g         4711 t
ORIGIN      
        1 atctttgtat agaaattggt gtattttttg gttttttatt ctgtcgcgat ttttgcattt
       61 tttaaccata agctaatgtg atgatcaatt ttaccttatg gttaacagtc tgtttcggtg
      121 gtaagttcag gcaaaagaga acgattgcgt tggggaccgg gagtggctcc gatgctgggt
      181 tttgtggtga taatttcacc atgaaaaagt tgtcagcccc gcttattcaa tgaggacaag
      241 atggcacaac agactccttt gtacgaacaa cacacgcttt gcggcgctcg catggtggat
      301 ttccacggct ggatgatgcc gctgcattac ggttcgcaaa tcgacgaaca tcatgcggta
      361 cgtaccgatg ccggaatgtt tgatgtgtcg catatgacca tcgtcgatct ccgcggcagc
      421 cgcacccggg agtttctgcg ttatctgctg gcgaacgatg tggcgaagct caccaaaagc
      481 ggcaaagccc tttactcggg gatgttgaat gcctctggcg gtgtgataga tgatctcatc
      541 gtctactact ttactgaaga tttcttccgc ctcgttgtta actccgccac ccgcgaaaaa
      601 gacctctcct ggattaccca acacgctgaa cctttcggca tcgaaattac cgttcgtgat
      661 gacctttcca tgattgccgt gcaagggccg aatgctcagg caaaagctgc cacactgttt
      721 aatgatgctc agcgtcaggc ggtggaaggg atgaaaccgt tctttggcgt gcaggtgggc
      781 gatctgttta ttgccaccac tggttatacc ggtgaagcgg gctatgaaat tgcgctgccc
      841 aatgaaaaag cggccgattt ctggcgtgcg ctggtggaag cgggcgttaa gccctgcggt
      901 ctgggggcgc gtgacacgct gcgtctggaa gcgggcatga atctttatgg tcaggagatg
      961 gacgaaacta tttctccttt agccgccaac atgggctgga ctatcgcctg ggaaccgaca
     1021 gatcgtgact ttatcggtcg tgaagccctg gaagtgcagc gtgagcatgg tacagaaaaa
     1081 ctggttggtc tggtgatgac cgaaaaaggc gtgctgcgta atgaactgcc ggtacgcttt
     1141 accgatgcgc agggcaacca gcatgaaggc attatcacca gcggtacttt ctccccgacg
     1201 ctgggttaca gcattgcgct ggcgcgcgtg ccggaaggta ttggcgaaac ggcgattgtg
     1261 caaattcgca accgtgaaat gccggttaaa gtgacaaaac ctgtttttgt gcgtaacggc
     1321 aaagccgtcg cgtgatttac ttttttggag attgattgat gagcaacgta ccagcagaac
     1381 tgaaatacag caaagaacac gaatggctgc gtaaagaagc cgacggcact tacaccgttg
     1441 gtattaccga acatgctcag gagctgttag gcgatatggt gtttgttgac ctgccggaag
     1501 tgggcgcaac ggttagcgcg ggcgatgact gcgcggttgc cgaatcggta aaagcggcgt
     1561 cagacattta tgcgccagta agcggtgaaa tcgtggcggt aaacgacgca ctgagcgatt
     1621 ccccggaact ggtgaacagc gaaccgtatg caggcggctg gatctttaaa atcaaagcca
     1681 gcgatgaaag cgaactggaa tcactgctgg atgcgaccgc atacgaagca ttgttagaag
     1741 atgagtaacg gctttattcc tcttctgcgg gagaggatca gggtgaggaa aatttatgcc
     1801 tcaccctcac tctcttcgta aggagagagg ttcacaattc actgcacgtt tcaggaacca
     1861 tcgctcatga cacagacgtt aagccagctt gaaaacagcg gcgcttttat tgaacgccat
     1921 atcggaccgg acgccgcgca acagcaagaa atgctgaatg ccgttggtgc acaatcgtta
     1981 aacgcgctga ccggccagat tgtgccgaaa gatattcagc ttgcgacacc accgcaggtt
     2041 ggcgcaccgg cgaccgaata cgccgcactg gcagaactca aggctattgc cagtcgcaat
     2101 aaacgcttca cgtcttacat cggcatgggt tacaccgccg tgcagctacc gccggttatc
     2161 ctgcgtaaca tgctggaaaa tccgggctgg tataccgcgt acactccgta tcagcctgaa
     2221 gtctcccagg gccgccttga agcactgctc aacttccagc aggtaacgct ggatttgact
     2281 ggactggata tggcctctgc ttctcttctg gacgaggcca ccgctgccgc cgaagcaatg
     2341 gcgatggcga aacgcgtcag caaactgaaa aatgccaacc gcttcttcgt ggcttccaat
     2401 gtgcatccgc aaacgctgga tgtggtccgt actcgtgctg aaacctttgg ttttgaagtg
     2461 attgtcgatg acgcgcaaaa agtgctcgac catcaggacg tcttcggcgt gctgttacag
     2521 caggtaggca ctaccggtga aattcacgac tacactgcgc ttattagcga actgaaatca
     2581 cgcaaaattg tggtcagcgt tgccgccgat attatggcgc tggtgctgtt aactgcgccg
     2641 ggtaaacagg gcgcggatat tgtttttggt tcggcgcaac gcttcggcgt gccgatgggc
     2701 tacggtggcc cacacgcggc attctttgcg gcgaaagatg aatacaaacg ctcaatgccg
     2761 ggccgtatta tcggtgtatc gaaagatgca gctggcaata ccgcgctgcg catggcgatg
     2821 cagactcgcg agcaacatat tcgtcgtgag aaagcgaact ccaacatttg tacttcccag
     2881 gtactgctgg caaacatcgc cagcctgtat gccgtttatc acggcccggt tggcctgaaa
     2941 cgtatcgcta accgcattca ccgtctgacc gatattctgg cggcgggcct gcaacaaaaa
     3001 ggtctgaaac tgcgccatgc gcactatttc gacaccttgt gtgtggaagt ggccgacaaa
     3061 gcgggcgtac tgacgcgtgc cgaagcggct gaaatcaacc tgcgtagcga tatcctgaac
     3121 gccgtgggga tcacccttga tgaaaccacc acgcgtgaaa acgtaatgca gcttttcagt
     3181 gtgctgctgg gcgataacca cgggctggag atcgacacgc tggacaaaga cgtggctcac
     3241 gacagccgct ctatccagcc tgcgatgctg cgcgacgacg aaatcctcac ccatccggtg
     3301 tttaatcgct atcacagcga aaccgaaatg atgcgctata tgcactcgct ggagcgtaaa
     3361 gatctggcgc tgaatcaggc gatgatcccg ctgggttcct gcaccatgaa actgaacgcc
     3421 gccgccgaga tgatcccaat cacttggacg gaatttgccg aactgcaccc gttttgcccg
     3481 ccggaacagg ctgaaggtta tcagcagatg attgcgcagc tggctgactg gctggtgaaa
     3541 ctgaccggtt acgacgccgt ttgtatgcag ccgaactccg gcgcacaggg cgaatacgcg
     3601 ggcctgctgg cgattcgtca ttatcatgaa agccgcaacg aagggcatcg cgatatctgc
     3661 ctgatcccgg cttctgcgca cggaactaac cccgcttctg cacatatggc aggaatgcag
     3721 gtggtggttg tggcgtgtga taaaaacggc aacatcgatc tgactgatct gcgcgcgaaa
     3781 gcggaacagg cgggcgataa cctctcctgt atcatggtga cttatccttc tacccacggc
     3841 gtgtatgaag aaacgatccg tgaagtgtgt gaagtcgtgc atcagttcgg cggtcaggtt
     3901 taccttgatg gcgcgaacat gaacgcccag gttggcatca cctcgccggg ctttattggc
     3961 gcggacgttt cgcacctcaa cctgcataaa actttctgca ttccgcacgg cggtggtggt
     4021 ccgggtatgg gaccgatcgg tgtgaaagcg catctggcac cgtttgtacc gggtcatagc
     4081 gtggtgcaaa tcgaaggcat gttaacccgt cagggcgcag tttctgcggc accgttcggt
     4141 agcgcctcta tcctgccaat cagctggatg tacatccgca tgatgggcgc agaagggctg
     4201 aaaaaagcaa gccaggtggc aatcctcaac gccaactata ttgccagccg cctgcaggat
     4261 gccttcccgg tgctgtatac cggtcgcgac ggtcgcgtgg cgcacgaatg tattctcgat
     4321 attcgcccgc tgaaagaaga aaccggcatc agcgagctgg acattgccaa gcgcctgatc
     4381 gactacggtt tccacgcgcc gacgatgtcg ttcccggtgg cgggtacgct gatggttgaa
     4441 ccgactgaat ctgaaagcaa agtggaactg gatcgcttta tcgacgcgat gctggctatc
     4501 cgcgcagaaa ttgaccaggt gaaagccggt gtctggccgc tggaagataa cccgctggtg
     4561 aacgcgccgc acattcagag cgaactggtc gccgagtggg cgcatccgta cagccgtgaa
     4621 gttgcggtat tcccggcagg cgtggcagac aaatactggc cgacggtgaa acgtctggat
     4681 gatgtttacg gcgaccgtaa cctgttctgc tcctgcgtac cgattagcga ataccagtaa
     4741 ttcactgatt cgagtatttt ctaaaggcgc ttcggcgcct ttttagtcag atgacaaagt
     4801 acaaaagtgc tcagacagtc ccctcgcccc tttggggaga gggttagggt gaggggaaca
     4861 ggccggcact ggcgcgaata tttaccctca ccccgaccct ctccctgaaa gggcgagggg
     4921 gaaaagcgtg ccaacattga agattgagcc agtttgttag caatctcaaa gatacgtcaa
     4981 cgaattaatt tttctcggaa aaacaaatgg ctatagcact tgtgaccggt ggcagtcgcg
     5041 gcatcgggcg ggcaactgca ttactgttgg cgcaagaagg gtatacggtg gcggttaatt
     5101 atcagcaaaa cctccacgcg gcgcaggaag tgatgaactt aataatgcaa gccggtggca
     5161 aagcattcgt gctccaggcg gatatcagcg acgaaaacca ggtcgttgcg atgtttacag
     5221 caatcgatca gcacgatgaa ccgctagcag cgctggtcaa taacgccggg atcttgttta
     5281 cccagtgcac cgttgaaaac cttaccgcag agcgaatcaa ccgagtactt tccaccaacg
     5341 tgacgggata ttttctctgc tgccgcgagg cggtaaaacg catggcgctt aaaaatggtg
     5401 gcagtggcgg cgctatcgtc aatgtctctt cggtggcctc acggttgggt tcgccagggg
     5461 aatatgttga ttacgcggca tcgaaagggg cgattgattc gttaaccacc ggactatcgc
     5521 tggaagtcgc cgcgcagggg atccgcgtta actgcgtgcg gccagggttt atttataccg
     5581 aaatgcacgc cagcggcggc gagcctggac gcgtcgatcg cgttaagtcg aacatcccca
     5641 tgcagcgtgg tggacaggca gaagaggtcg cgcaggccat tgtctggcta ctaagtgata
     5701 aagcctctta cgtcacggga agttttatcg atttggcggg cgggaaataa aacagggaag
     5761 ttgtctgacc ggatgcaaca agtattgcat ccggtacttc atcgacttaa agcttctcgc
     5821 cgttgctggc aatcacctct ttgtaccagt taaagctctt cttacgtgaa cgcgacatat
     5881 cgccagtacc gtcgtcatgt ttattcacat agataaagcc gtagcgtttg ctgtactgcc
     5941 cggtagtgaa tgacacgcag tcgatgcagc cccacggtgt gtagcccatc agatccacac
     6001 catcgtaagt cactgctttt ttcatctctt caatatgggc gcgcaggtag tcaatgcggt
     6061 agtcgtcgtt gatgctgcca tcttcttcca ctttgtcgta agcgccaaaa ccgttttcga
     6121 caataaacag cggcctctga taacgctcat acagttcgca aagggcatag cgcagaccta
     6181 ctggatcaat ctgccagccc cagtcagacg ctttaacata cgggttcggt acgctgcctt
     6241 caaaaccaga gatcgcatcg ccggtgccgc cttcggcctt cactgcgttg gtcatgtaat
     6301 agctgaaacc aagataatcg caggtgcctt cgcgcagtac atcgagatcg ccgtcttcca
     6361 ttttgatatt aaatccgcga cgttcccact cgttcaatac ataggacggg taatagccgc
     6421 atagctgcac atcggtaaag acgtagcgtt cgcgcatcga ctcctgagcg aacatcacat
     6481 cgtccgggtt acatgagtag ggatagagcg gcaccatcgc cagcatacag ccgactttca
     6541 tctccgggtt aatgcgacgc gcggctttca ctgccagggc gctggcgaca aactggtgat
     6601 gcagcacctg gtacatcgtc tcttccgggt tgtcatgctc ggtatacacc acgccggagc
     6661 agcagtaacc aaacagcggc gcacgccagt tacgctggtt gtttatttcg ttgaaggtca
     6721 tccagtattt gactttatgc ttgtagcgtt cgaataccac ttccgcgaaa cggacaaaga
     6781 aatcaacgaa tttacggttg gtccagctac cgtattgctg caccagatgc agcggcattt
     6841 caaagtggga gagggttatc accggttcga tgttgtattt cagcagttca tcgaacagag
     6901 agtcgtaaaa cttcagccct tcttcgtttg gctgagcttc atcacctttt ggaaaaatac
     6961 gcgtccaggc gatggaagtc cgaaaacatt tgaagcccat ttcggcaaat agcttgatgt
     7021 cttccttgta gtgaccataa aaatcaacgg cttcatggtt tggatagtat tttcctggca
     7081 agacttcttt ggtgatttcg cgcggcacgc cgtgtgcgcc acaggtcaga acgtcacaaa
     7141 tgctcggtcc tttgccgcct ttgttccagc cgccttcgac ctgatgagcg gcaacggcgc
     7201 caccccataa gaaatctttc ggtaaggtga gttttttcac tatcataggc tccagatcag
     7261 tcaattctta caggaaatag taccatgcag ccaaacgaca tcactttttt caacgtttcc
     7321 aggatgacat tctggctggg cgtaaaacca tcaccatccg cgacgagtct gaatcgcact
     7381 tcaaaacggg tgatgtgctt cgtgtcggac gttttgaaga tgacggttat ttttgcacga
     7441 ttgaagtcac cgcaacctca accgtaacgc tggatacact gacggaaaaa catgcagagc
     7501 aggaaaatat gaccctgcct gaactgaaaa aggtcattgc tgacatctat cccggtcaga
     7561 cacaatttta tatgattgaa tttaaatgtc tttaaattga actcacgcct gttagttgaa
     7621 atttcgcatt taatttcatg atttttagga gattagtctc aaatcagaat ttattttagc
     7681 taacaggtgt tcactggaac tattctcagt tacgctggag aggtatacat gtgcgtgtac
     7741 gtttctctgg agtaagttat ggttcagaag cccctcatta agcagggata ttcgctggca
     7801 gaggaaattg ccaacagcgt cagtcacggc attgggctgg tgtttggtat cgttgggctg
     7861 gtgttgctac tggttcaggc ggtggatctc aatgccagcg ccacagcgat aaccagttac
     7921 agcctctatg gcggcagtat gatcctgctg ttcctcgctt cgacgctcta tcacgccatt
     7981 cctcatcaac gggcaaaaat gtggctgaag aaatttgacc attgcgctat ttacctgttg
     8041 attgccggaa cctacacgcc gtttttgctg gtggggctgg attctccgtt agcgcgcggg
     8101 ttgatgattg ttatctggag cctagcattg ctggggattc tgttcaaact gaccatcgcg
     8161 caccgattca aaattttatc tctggtgact tatctggcga tgggctggct gtcgctggtg
     8221 gtaatttatg aaatggcagt taagctcgcg gcgggcagcg ttaccttact ggcggtaggc
     8281 ggcgtggttt attcgctcgg ggtgattttc tacgtctgca aacgcattcc atacaaccat
     8341 gccatctggc acggcttcgt gctcggcggt agtgtgtgcc actttctggc gatctatttg
     8401 tatattgggc aggcgtaatg ggtatgcctt tgtaggcctg ataagacgcg tcagcgtcgc
     8461 atcaggcatc ggagcgcaac cgccggatgc ggcgtgaacg ccttatccga tctacgggcc
     8521 tcgtccctgt tgtgataaag gcatcaaatc ccagacatga taagacgcgt cagcgtcgca
     8581 tcaggcgata aatgcctgat gcgatacaga gatttactct tcgagcgaat acggcagcgg
     8641 ctcgatatgc agtgtattcg catcgtcgcg tacgcggaag atgctgtccg gttccatatc
     8701 gttattcatg acgacctgta ccacgacctg accatcttcc agttttaccg cagccagcac
     8761 cgtaccggta cgacgccagt tctcgcccat tttcagctct aagtcttcac cagcttccgg
     8821 cagtcggctg gcactacctg ccagcaacca gagcgcgcgt ttgttggcac cacggaattt
     8881 tgctcgcgcc accatctctt gtccggtata acagcctttc ttaaagctga taccgcccag
     8941 cgcctggaga ttggtcgcct gtgggataaa ctgcccgctg ttggcggcat caatcaccgg
     9001 gaaaccagct tcaatgttta atgccagcca ctgttggcta ttgttcagtt ccgcttcacc
     9061 gcgcagttta tcggtcagca tattagcagt agcttcatcg gttacgatca ggaaacgttc
     9121 tgccgggtgt tcaaaccata gcaaagtggt cgcgccttct ttgactacct gtttttcttt
     9181 cgaaggcagt tcgctaaaga gatttgccag cgcggcgcgc gcctgaaaac cggcaacacc
     9241 aagcagcaca cgctcgtcgt ctggcgcgat ggtcacttta gagaataccg catatttttt
     9301 cagttcagtc agctgcggtt cacgcacgct gcgccgttca atccatgcaa agccatcgcc
     9361 gtcgcggaac agacgtaaat tgctccacat tttacctttg gcgtcgcaat gggcggcgag
     9421 caggtgctga tcttctgcca tctggctgac atctgctgtc acctgaccct gcatatattt
     9481 ttcgctgtcc gcgccagtaa tggtggcaag cgcccagtca tcaagcgtca tcagcgtcag
     9541 cggtaaacgg gcagaagccg taggctgacg tggaggaaaa ggtgtaaaag ccataataga
     9601 gtcctgagtt gcttaacggc gtattaatga ctaatggtaa aagagctatg aggcaatgca
     9661 agtgctttca ctacgcgatc tgtgccttct gcgaggcgac tgccaaaaaa atgaagccct
     9721 tgaattgttg tgaattaatt tcgcgatcgc gatggtgttt actgatattc ccgtaaaaga
     9781 agtcgaaaaa cacgttacaa tagccgggta ctggattttc gtgagacagg aagaagatgg
     9841 acattaacaa caaagcccga attcattggg catgccgccg tggtatgcgc gaactcgata
     9901 tttcaatcat gtcgtttttc gaacatgagt acgacagctt aagcgatgac gaaaaacgca
     9961 tctttattcg tctgctggaa tgtgacgatc cggacctgtt taactggctg atgaatcacg
    10021 gtaaaccggc cgatgcagaa ctggaaataa tggtccgact catccagaca cggaaccggg
    10081 aacgtggtcc tgtggcaatc tgatttgcgc gtctcctggc gcgcacagtg gctttccttg
    10141 ctgattcatg ggctggttgc cgctgttatt ttactcatgc cctggccgct cagttacacc
    10201 ccgttatgga tggtgttact ttcgctggtg gtatttgatt gcgttcgcag ccagcggtgt
    10261 attaatgctc gccaggggga aattcgcttg ttgatggacg ggcgtttgcg ttggcaaggg
    10321 caggagtgga gcatcgtcaa agcaccgtgg atgattaaga gcggcatgat gctgcgttta
    10381 cgttctgatg gcggtaaacg gcaacattta tggctggcag ccgacagcat ggacgaagcc
    10441 gaatggcggg atttacggcg gattttgttg caacaagaga cgcaaagata aagatgacgg
    10501 caaagcatat gcgctaagcc gtcagtgaga tcaggcgtaa tgctctgcca tttcgttgag
    10561 gatttgctcg caccagctct gaatacgctc gtcgctaagg tcatactggt tagtttcatc
    10621 cagcgccaga cccacgaaca gttgcccgtc agcaatcacc ggtttcgggc tggtaaattc
    10681 atatccttcc gttggccagt agccgacgaa cttcacgcct ttggtcgaga gtttgtcatg
    10741 cagcataccg agcgcatcga ggaaccactc gccgtatccc agttgatcgc caagcccata
    10801 cagcgcaata attttacctt caaggttcag gtcgtcgagc tgatcccaga cggcttccca
    10861 gtcttcctgg atttcaccaa aatcccaggt cgggataccc agaatgagca catcgtactg
    10921 ctccattaat ttcggggagt cgtccttgag gttatgtaag gtcaccagtt ctgggccgat
    10981 aatatcgcgg attttttctg ccgccatttc ggtgtaacag gtgctggaac cgtaaaaaag
    11041 acccatattc ataacgtaag cttctcgatt ctgctgtggc ttttgcagct agtgtatcag
    11101 aatcgcttta gtcacggcac aataaggcat aatggatcaa atgagtgacc ataaggggcg
    11161 caagtgaaac aggagctggc acgcatcgag cagtttcttg atgctctgtg gctggaaaaa
    11221 aatctggctg aaaatacgtt gaacgcttac cgtcgcgatc tgtcaatgat ggtggagtgg
    11281 ttgcatcacc gcgggttgac gctggcgacg gcgcaaagtg acgatttgca ggcattactg
    11341 gcagaacggc tggagggcgg gtataaagcc accagctcag cgcgtttgct gagtgcagtg
    11401 cgccgattgt tccagtatct ttatcgcgaa aagtttcgtg aagacgatcc cagtgcgcat
    11461 ctcgcttcac cgaaattgcc ccagcgtttg ccaaaagatt taagcgaagc gcaggtcgaa
    11521 cgtttattac aggcgccatt aattgatcag ccactggagc tacgcgataa agccatgctt
    11581 gaagtgttgt atgctaccgg gctgcgtgtc tctgaactgg tcgggctgac aatgagtgat
    11641 atcagcctgc gtcagggcgt agtgcgggtc attggtaaag gcaacaaaga gcgcctggtg
    11701 ccgttagggg aagaggcggt ttactggctg gaaacctatc tggaacatgg gcgtccgtgg
    11761 ctgctgaatg gtgtgtcaat tgacgtgttg tttcccagcc agcgtgcgca gcagatgacg
    11821 cgacagacct tctggcaccg cattaaacat tatgcagtgc tggcgggtat cgacagcgaa
    11881 aagctgtcac cgcatgtgtt gcgtcacgct tttgccactc atttattaaa tcatggtgcg
    11941 gatttacgcg tggtgcagat gctactgggc cacagcgatc tttccaccac gcaaatttat
    12001 acgcatgtcg ctaccgagcg tctgcggcaa cttcatcaac agcatcaccc gcgggcgtga
    12061 tgtctgaaaa gaacgggaag atttatgaag aaaggtttta tgttgtttac tttgttagcg
    12121 gcgttttcag gctttgctca ggctgatgac gcggcaattc aacaaacgtt agccaaaatg
    12181 ggcatcaaaa gcagcgatat tcagcccgcg cccgtagctg gcatgaagac agttctgact
    12241 aacagcggtg tgttgtacat caccgatgat ggtaaacata tcattcaggg gccaatgtat
    12301 gacgttagtg gcacggctcc ggtcaatgtc accaataaga tgctgttaaa gcagttgaat
    12361 gcgcttgaaa aagagatgat cgtttataaa gcgccgcagg aaaaacacgt catcaccgtg
    12421 tttactgata ttacctgtgg ttactgccac aaactgcatg agcaaatggc agactataac
    12481 gcgctgggga tcaccgtgcg ttatcttgct ttcccgcgcc aggggctgga cagcgatgca
    12541 gagaaagaaa tgaaagctat ctggtgtgcg aaagataaaa acaaagcgtt tgatgatgtg
    12601 atggcaggta aaagcgtcgc accagccagc tgcgacgtgg atattgccga ccattacgca
    12661 cttggcgtcc agcttggcgt tagcggtact ccggcagttg tgctgagcaa tggcacactt
    12721 gttccgggtt accagccgcc gaaagacatg aaagaattcc tcgacgaaca ccaaaaaatg
    12781 accagcggta aataattcgc gtgaaacaac agatacaact tcgtcgccgt gaagtcgatg
    12841 aaacggcaga cttgcccgct gaattgcctc ccttgctgcg ccgtttatac gccagccggg
    12901 gagtacgcag tgcgcaagaa ctggaacgca gtgttaaagg tatgttgccc tggcagcaac
    12961 taagcggtgt cgaaaaggcc gttgagatcc tttacaacgc ttttcgcgaa ggaacgcgga
    13021 ttattgtggt cggtgatttc gacgccgacg gcgcgaccag cacggctcta agcgtgctgg
    13081 cgatgcgctc gcttggttgc agcaatatcg actacctggt accaaaccgt ttcgaagacg
    13141 gttacggctt aagcccggaa gtggtcgatc aggcccatgc ccgtggcgcg cagttaattg
    13201 tcacggtgga taacggtatt tcctcccatg cgggagttga gcacgctcgc tcgttgggca
    13261 tcccggttat tgttaccgat caccatttgc cgggcgacac attacccgca gcggaagcga
    13321 tcattaaccc taacttgcgc gactgtaatt tcccgtcgaa atcactggca ggcgtgggtg
    13381 tggcgtttta tctgatgctg gcgctgcgca cctttttgcg cgatcagggc tggtttgatg
    13441 agcgcggcat cgcaattcct aacctggcag aactgctgga tctggtagca ctggggacag
    13501 tagcggacgt cgtgccgctg gacgctaata atcgcattct gacctggcag gggatgagtc
    13561 gcatccgtgc cggaaagtgc cgtccaggga ttaaagcgct gctggaagtg gcaaatcgcg
    13621 atgcacaaaa actagccgcc agcgatttag gttttgcgct gggaccgcgt ctcaatgctg
    13681 ccgggcgact ggatgatatg tccgttggtg tggcgctgtt gctgtgcgac aacatcggcg
    13741 aagcgcgcgt gctggcaaat gaactcgatg cgctaaacca gacgcgaaaa gagatcgaac
    13801 aaggaatgca ggttgaagcc ctgaccctgt gcgagaaact ggagcgcagc cgtgacacgc
    13861 tacccggcgg gctggcaatg tatcaccccg aatggcatca gggcgttgtc ggtattctgg
    13921 cttcgcgtat caaagagcgt tttcaccgtc cggttatcgc ctttgcgcca gcaggtgacg
    13981 gtacgctgaa aggttccggt cgctccattc aggggctgca tatgcgtgat gcgctggagc
    14041 gattagacac actctacccc ggcatgatgc tcaagtttgg cggtcatgcg atggcggcgg
    14101 gtttgtcgct ggaagaggat aaattcgaac tctttcaaca acggtttggc gaactggtta
    14161 ctgagtggct ggccccttcg ctattgcaag gcgaagtggt gtcagacggt ccgttaagcc
    14221 cggccgaaat gaccatggaa gtggcgcagc tgctgcgcga tgctggcccg tgggggcaga
    14281 tgttcccgga gccgctgttt gacggtcatt tccgtctgct gcaacagcgg ctggtgggcg
    14341 aacgtcattt gaaggtgatg gtcgaaccgg tcggcggcgg tccactgctg gatggtattg
    14401 cttttaatgt cgataccgct ctctggccgg ataacggcgt gcgcgaagtg caactggctt
    14461 ataagctcga tatcaacgag tttcgcggca accgcagcct gcaaattatc atcgacaata
    14521 tctggccaat ttagcgtcat cttctctata aaaaagagcg tggattgggt acaatcccgc
    14581 tcttatcacc gcattttgac tagctcaata aaagaaatca gaccatgttt gaaattaatc
    14641 cggtaaataa tcgcattcag gacctcacgg aacgctccga cgttcttagg gggtatcttt
    14701 gactacgacg ccaagaaaga gcgtctggaa gaagtaaacg ccgagctgga acagccggat
    14761 gtctggaacg aacccgaacg cgcacaggcg ctgggtaaag agcgttcctc cctcgaagcc
    14821 gttgtcgaca ccctcgacca aatgaaacag gggctggaag atgtttctgg tctgctggaa
    14881 ctggctgtag aagctgacga cgaagaaacc tttaacgaag ccgttgctga actcgacgcc
    14941 ctggaagaaa aactggcgca gcttgagttc cgccgtatgt tctctggcga atatgacagc
    15001 gccgactgtt acctcgatat tcaggcgggg tctggcggta cggaagcaca ggactgggcg
    15061 agcatgcttg agcgtatgta tctgcgctgg gcagaatcgc gtggtttcaa aactgaaatc
    15121 atcgaagagt cggaaggtga agtggcgggt attaaatccg tgacgatcaa aatctccggc
    15181 gattacgctt acggctggct gcgtacagaa actggcgttc accgcctggt gcgtaaaagc
    15241 ccgtttgact ccggcggtcg tcgccacacg tcgttcagct ctgcgtttgt ttatccggaa
    15301 gttgatgatg atattgatat cgaaatcaac ccggcggatc tgcgcattga cgtttatcgc
    15361 gcgtccggcg cgggcggtca gcacgttaac cgtaccgaat ctgcggtgcg tattacccac
    15421 atcccgaccg ggatcgtgac ccagtgccag aacgaccgtt cccagcacaa gaacaaagat
    15481 caggccatga agcagatgaa agcgaagctt tatgaactgg agatgcagaa gaaaaatgcc
    15541 gagaaacagg cgatggaaga taacaaatct gacatcggct ggggcagcca gattcgttct
    15601 tatgtccttg atgactcccg cattaaagat ctgcgcaccg gggtagaaac ccgcaacacg
    15661 caggccgtgc tggacggcag cctggatcaa tttatcgaag caagtttgaa agcagggtta
    15721 tgaggaacca acatgtctga acaacacgca cagggcgctg acgcggtagt cgatcttaac
    15781 aatgaactga aaacgcgtcg tgagaagctg gcgaacctgc gtgagcaggg aattgccttc
    15841 ccgaacgatt tccgtcgcga tcatacctct gaccaattgc acgcagaatt cgacggtaaa
    15901 gagaacgaag aactggaagc gctgaacatc gaagtcgccg ttgctggccg catgatgacc
    15961 cgccgtatta tgggtaaagc gtctttcgtt accctgcagg atgtgggtgg ccgcattcag
    16021 ctgtacgttg cccgtgacga tctgccggaa ggcgtttaca acgagcagtt caaaaaatgg
    16081 gacctcggcg acatcctcgg cgcgaaaggt aaactgttca aaaccaaaac cggcgaactg
    16141 tctatccact gtaccgagct gcgtctgctg accaaagcac tgcgtccgct gccggataaa
    16201 ttccacggct tgcaggatca ggaagcgcgc tatcgtcagc gttatctgga tctcatctct
    16261 aacgatgaat cccgtaacac ctttaaagta cgctcgcaga tcctctctgg tattcgccag
    16321 ttcatggtga accgcggctt tatggaagtt gaaacgccga tgatgcaggt gatcccaggc
    16381 ggtgccgctg cgcgtccgtt tatcacccac cataacgcgc tggatctcga catgtacctg
    16441 cgtatcgcgc cggaactgta cctcaagcgt ctggtggtcg gtggcttcga gcgtgtattc
    16501 gaaatcaacc gtaacttccg taacgaaggt atttccgtac gtcataaccc agagttcacc
    16561 atgatggaac tctacatggc ttacgcagat tacaaagatc tgatcgagct gaccgaatcg
    16621 ctgttccgta ctctggcaca ggatattctc ggtaagacgg aagtgaccta cggcgacgtg
    16681 acgctggact tcggtaaacc gttcgaaaaa ctgaccatgc gtgaagcgat caagaaatat
    16741 cgcccggaaa ccgacatggc ggatctggat aacttcgact ctgcgaaagc gattgctgaa
    16801 tctatcggta tccacgttga gaagagctgg ggtctgggcc gtatcgttac cgagatcttc
    16861 gaagaagtgg cagaagcaca tctgattcag ccgaccttca ttactgaata tccggcagaa
    16921 gtttctccgc tggcgcgtcg taacgacatt aacccggaaa tcacagaccg ctttgagttc
    16981 ttcatcggtg gtcgtgaaat cggtaacggc tttagcgagc tgaatgacgc ggaagatcag
    17041 gcgcaacgct tcctggatca ggttgccgcg aaagacgcag gtgacgacga agcgatgttc
    17101 tacgatgaag attacgttac cgcactggaa catggcttac cgccgacagc aggtctgggg
    17161 attggcatcg accgtatggt aatgctgttc accaacagcc ataccatccg cgacgttatt
    17221 ctgttcccgg cgatgcgtcc ggtgaaataa gcattacgtt atgctcacaa ccccggcaaa
    17281 tgtcggggtt ttttatttaa gctgggtaaa tgcagataat cgtattctgg cttcgcgatt
    17341 tgtcgcctgc atcaccatcc acggactgaa cgcccacggc gtggcatcaa taccgcgtaa
    17401 tacatcagct aaatcacacc attgataatc catcacttca tcatcgttga tctgtaacgc
    17461 actggtggtg cgtgcggcaa ataccggaca cacttcattt tccacaatgc ctctcggatc
    17521 ggtggcgcgg tagcgaaagt caggatagat agattcagga ggcgtaattt ccacgccaag
    17581 ctcataacgg caacggcgga tcaccgcgtc ttcgctgctt tctcccagtt gtgggtgccc
    17641 acaaaccgag ttagtccaca cgccaggcca cgcttttttg ctcagtgcgc ggcgggtaac
    17701 taataattgc cccttggcat taaacagcca actggagaac gcgagatgta agagggtgtc
    17761 tgccgtgtgt gcggcatact tttccagcgt acccgtggga actccctgtg cattcaataa
    17821 aatgacgtgt tccgtttgca taatttctca catgtaattc tgatcactca tcattataga
    17881 taattgaaga gtgaaatgat taaccaagag agcgtttatc gacaaccgcc cgttttgagc
    17941 ggatgccgac aggtaaaaac catttaaaaa agtagcatca tatatttgaa gcgactttca
    18001 atggccattg caacacagtc agaaatgaca ggaatagaga gagtaaaaag caacaacaga
    18061 ctgaatatag cgcgcattaa aaaattcata ttattctcca tgctcatttt tcttcagctt
    18121 gcgcatcaga agccacatac gtactgtcct gacttctaaa tctttatcag agacggattc
    18181 cttcaccagg tcagcatgat gctgatagcc attaaagaag acgttcagca caacggcgct
    18241 aagggttgcg agcataatgc cgctatgtag cagcggttgc agtacggccg gtaacttaga
    18301 aaagaaatcg tgagagagcg tcggagtcat gccaacgccg agactgatcg ccacaatata
    18361 gaggttgtaa cggttggtgg tgtagttaca gcgcgacaga attcgaatcc ctgtcgccag
    18421 taccatgccg aacatcacta gaccagcgcc gcccagcaca aattgcggaa tggaggctac
    18481 cagcaccgcc atttttggca ccatgccaaa caggattaat ataattcccg aagaaataca
    18541 cacccagcgg ctatgaacgc gcatcacgct aaccaggcca acgttttgag aaaatgacgt
    18601 gtgggggaag ctgttaaacg tgccgcctat cattgtccct acgccatcga cacgcagccc
    18661 gcaaataata tcgtgtgaag agagtttacg accgactatt tcacccagtg ccaggaacat
    18721 ccccatcgac tcgataaaca cgatgattaa cacggcagtc atggtcagaa tggaaacggg
    18781 atcgaaaatc ggcataccaa acgacatcgg ggtgacaatc gcaaaccacg aggcatcatg
    18841 tagcccggat aaattgactt cattcatcat ccacgaaagt aaaaagccaa atacaatccc
    18901 cagtaatacg gcgacgttgg acataaatcc tttcgcatag cgagtaatga gcaagataaa
    18961 aattaagacg gcaaaggaga tacctaaata aacgggatta ccatattgcg gattcccttt
    19021 acctccagcg gcccagtcaa tacccacctg aatgatgcta agcccaatag aagtaataac
    19081 cacaccggta accagtggcg ggaataaagg catcaagcga ccaataagtg gcgctaataa
    19141 tgtggtgata aaacccgcgg cgatagtggc accaaatatc cccagcaggc cgatatccgg
    19201 gttcatccca atggctatca ttggtgttac agcagcaaag gtcaccgaca taatcaccgg
    19261 caggcggatc cccataaagc ggccgatacc gatacattgc aataatgtga cgatcccgca
    19321 gcaaaagaga tccgagctaa tgagcatcgc aatagcatct tttgagaggc ccagtcggtc
    19381 accaatcata agaggaacag cgactgcacc tgcgtacatg acaaggacgt gttgcagacc
    19441 gagtatgatc agctttcctg gtgataatat gcggtcaacc tcatcagttg ggcggtcttg
    19501 cccagaagat gagggaagtt gggaatctat ggcgctcatc agtgtttctc ctttcgcccg
    19561 ccagaataaa aagcaaccgt ttacgttaat gaaactgtct aaacacatgc tcgtggagtg
    19621 ccagtttttg cgaggaaggg gagttagaga caggaattgc ggaggcaatc acataatcgt
    19681 gcttaaacca cgaaaatgac aagagaaact gacaatggaa ttatctgtaa ttttctcata
    19741 ttgatatttc attaatcatt ctggcatgtc gcactctcgc atttaatcgt ttttatctgg
    19801 atagcgctct tttgatcggc aatgttgaaa taatgaaagc ggcatagatt gaggggcaat
    19861 aatttgaact cagtcgcaaa attataatta aaacggtcat gctacctaat atatgcccgt
    19921 ttattgcatc gcgaaatttg agaagacata actcattaaa tgaaagggat gcaaatgaat
    19981 acgtttatcg ctgctgaagc tgcggaatgt ataggctgtc
//
LOCUS       DAZSIO010000069        19698 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZSIO010000069 DAZSIO010000000
VERSION     DAZSIO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.069"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACHX5T_002223"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACHX5T_002223"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR8467410.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACHX5T_002224"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACHX5T_002224"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR8467411.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACHX5T_002225"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACHX5T_002225"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR8467412.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="ACHX5T_002226"
     CDS             6038..6658
                     /locus_tag="ACHX5T_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR8467413.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="ACHX5T_002227"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="ACHX5T_002227"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR8467414.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="ACHX5T_002228"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="ACHX5T_002228"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR8467415.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="ACHX5T_002229"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="ACHX5T_002229"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR8467416.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="ACHX5T_002230"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="ACHX5T_002230"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR8467417.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10068..11069)
                     /locus_tag="ACHX5T_002231"
     CDS             complement(10068..11069)
                     /locus_tag="ACHX5T_002231"
                     /inference="COORDINATES: protein motif:HMM:NF007315.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8467418.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     ELLYRSMKFVSARNFWRHGASNGWASPMKAIRSCRSTRNHSGSFSRFLLNFVQIFVS"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="ACHX5T_002232"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="ACHX5T_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="ACHX5T_002233"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="ACHX5T_002233"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR8467419.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="ACHX5T_002234"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="ACHX5T_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR8467420.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="ACHX5T_002235"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="ACHX5T_002235"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR8467421.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="ACHX5T_002236"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="ACHX5T_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR8467422.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="ACHX5T_002237"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="ACHX5T_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR8467423.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..19633
                     /gene="rpoD"
                     /locus_tag="ACHX5T_002238"
     CDS             17792..19633
                     /gene="rpoD"
                     /locus_tag="ACHX5T_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR8467424.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4683 a         5506 c         5031 g         4478 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtatcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcaccgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    19261 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    19321 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    19381 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    19441 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    19501 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    19561 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    19621 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc
    19681 tgcacaaacg ccaccttt
//
LOCUS       DAZSIO010000070        19037 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZSIO010000070 DAZSIO010000000
VERSION     DAZSIO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.070"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            483..932
                     /gene="alaE"
                     /locus_tag="ACHX5T_002239"
     CDS             483..932
                     /gene="alaE"
                     /locus_tag="ACHX5T_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR8467425.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(969..1313)
                     /locus_tag="ACHX5T_002240"
     CDS             complement(969..1313)
                     /locus_tag="ACHX5T_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR8467426.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1465..1794
                     /locus_tag="ACHX5T_002241"
                     /pseudo
     CDS             1465..1794
                     /locus_tag="ACHX5T_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
     gene            2042..2287
                     /gene="nrdH"
                     /locus_tag="ACHX5T_002242"
     CDS             2042..2287
                     /gene="nrdH"
                     /locus_tag="ACHX5T_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR8467427.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            2284..2694
                     /gene="nrdI"
                     /locus_tag="ACHX5T_002243"
     CDS             2284..2694
                     /gene="nrdI"
                     /locus_tag="ACHX5T_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFR8467428.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            2667..4811
                     /gene="nrdE"
                     /locus_tag="ACHX5T_002244"
     CDS             2667..4811
                     /gene="nrdE"
                     /locus_tag="ACHX5T_002244"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR8467429.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4821..5780
                     /gene="nrdF"
                     /locus_tag="ACHX5T_002245"
     CDS             4821..5780
                     /gene="nrdF"
                     /locus_tag="ACHX5T_002245"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR8467430.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            6135..7337
                     /gene="proV"
                     /locus_tag="ACHX5T_002246"
     CDS             6135..7337
                     /gene="proV"
                     /locus_tag="ACHX5T_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR8467431.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            7330..8394
                     /gene="proW"
                     /locus_tag="ACHX5T_002247"
     CDS             7330..8394
                     /gene="proW"
                     /locus_tag="ACHX5T_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR8467432.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8452..9444
                     /gene="proX"
                     /locus_tag="ACHX5T_002248"
     CDS             8452..9444
                     /gene="proX"
                     /locus_tag="ACHX5T_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR8467433.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9636..10815
                     /locus_tag="ACHX5T_002249"
                     /pseudo
     CDS             9636..10815
                     /locus_tag="ACHX5T_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            10939..11676
                     /gene="ygaZ"
                     /locus_tag="ACHX5T_002250"
     CDS             10939..11676
                     /gene="ygaZ"
                     /locus_tag="ACHX5T_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR8467434.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11666..12001
                     /gene="ygaH"
                     /locus_tag="ACHX5T_002251"
     CDS             11666..12001
                     /gene="ygaH"
                     /locus_tag="ACHX5T_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR8467435.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            12092..12622
                     /gene="emrR"
                     /locus_tag="ACHX5T_002252"
     CDS             12092..12622
                     /gene="emrR"
                     /locus_tag="ACHX5T_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR8467436.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12749..13921
                     /gene="emrA"
                     /locus_tag="ACHX5T_002253"
                     /pseudo
     CDS             12749..13921
                     /gene="emrA"
                     /locus_tag="ACHX5T_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            13938..15476
                     /gene="emrB"
                     /locus_tag="ACHX5T_002254"
     CDS             13938..15476
                     /gene="emrB"
                     /locus_tag="ACHX5T_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR8467437.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15540..16055)
                     /gene="luxS"
                     /locus_tag="ACHX5T_002255"
     CDS             complement(15540..16055)
                     /gene="luxS"
                     /locus_tag="ACHX5T_002255"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR8467438.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(16205..17761)
                     /gene="gshA"
                     /locus_tag="ACHX5T_002256"
     CDS             complement(16205..17761)
                     /gene="gshA"
                     /locus_tag="ACHX5T_002256"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR8467439.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(17834..18262)
                     /locus_tag="ACHX5T_002257"
     CDS             complement(17834..18262)
                     /locus_tag="ACHX5T_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR8467440.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(18259..18825)
                     /gene="yqaB"
                     /locus_tag="ACHX5T_002258"
     CDS             complement(18259..18825)
                     /gene="yqaB"
                     /locus_tag="ACHX5T_002258"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR8467441.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4560 a         4912 c         4792 g         4773 t
ORIGIN      
        1 aggttattgg tgaacagagt ggcgctctct gctcgctaac tccgcaagcc tgacaaaacc
       61 gaagtgaata aaaatgcaat ataacagaat aaaagaaaac acccctcact ggtgctttct
      121 gctgttaatg tacattttct ggcatcaaaa atgctgtaaa tgacccggtc atgacaaaca
      181 ccgaattctc tacgcttata gcgttacctc ccccccaaac tttcattagt gaaattaaag
      241 gcaagatcat atctaattga tttaattgaa aataaatcat attggcatgt aaaaatccac
      301 aatgtacaaa aaacgagccg ttacggaata ttttatctac aaaaactaac taaataaaaa
      361 tttttcacta attgattagt catagacagc gatatacgct atgcaaaaat gcagatggca
      421 atgagatcca ctgctttcat ctccattaac atcccattac gcttttatta aggagcatta
      481 gcatgttctc accgcagtca cgcttgcgtc atgcagttgc agatacgttc gcgatggttg
      541 tttactgttc tgtcgtgaac atgtgtattg aagttttcct ctccggaatg agcttcgaac
      601 agtcttttta ttccagattg gtagcgattc cggtgaacat cttaattgca tggccatacg
      661 gtatgtaccg cgatctgttt atgcgcgcgg cacgcaaagt tagcccgtcg ggctggataa
      721 aaaatctggc tgatatcctg gcttatgtga cgttccagtc accggtgtat gtggcgatct
      781 tgttagtggt gggcgcagac tggcatcaga ttatggcggc ggtcagttca aacatcgttg
      841 tttcgatgtt gatgggggcg gtttatggct acttcctcga ttattgccgc cgactgttta
      901 aagtcagccg ttaccagcag gtaaaagcct gactcttctt ttcgcgactg gcatcgccag
      961 tcgcgtcttt aactggcttc gccaaataaa ccattcaaat aacgttcaag cgcaacacgc
     1021 gaactaaagc catgcgggat accgtaatgc tcgtgtcgct cgcctgctaa atagagcgga
     1081 aattgctggt aatgatcgca agttttaata tcggaagcgg gttcagccag tgttgaactg
     1141 cgttctttta atgttggatg aattaccaga gcggtacgtc ccattcgcgc ttcgcgatta
     1201 acatagacat aattttcccc acggcggtaa ccatacgctt tctgggttac cacatcgaca
     1261 gtaaaaccga ctttttcaag tacgcgcgcc acctcgtctg gtcgtaaata catatttgat
     1321 cctcgttatt ttcaaccgcg ggcctacctt acctgattgc gcattcacaa cgctttcaga
     1381 aaagtccata aagccgcgat gaacctcagt taagaaaatt atggtctaca ctgaaaatta
     1441 catcgaattc taatggggga tcatatgttt aaccgaccga accgcaacga cgttgatgat
     1501 ggcgtgcagg atatccagaa tgatgtcaat caattagctg acagcctcga atctgtattg
     1561 aagtcctggg gcagcgacgc caaaggggaa gctgaagccg cacgcagtaa agctcaggcg
     1621 ttgctgaaag aaaccagggc gcgaatgcat ggtcgtactc gcgtccagca agccgcgcgc
     1681 gatgccgttg gctgagcgga ttcttttgtt cgtgaaagac cctggtgtag cgtgggtaca
     1741 gcagctgcgg taggtatttt tatcggcgca ctgttaagca tgcgcaaatc gtagtgcaaa
     1801 aatgataata aatacgcgtc tttgaccccg aagcctgtct tcggggtttc tttttgcctg
     1861 gtgaatcaca aaaatccccc tcccccgtca cgctcatatc cagggtaatt tcgatcacta
     1921 tttgctatat attgtgtagt tgaatctttt ttcaactaca tctagtatct ctgtatcaac
     1981 agagagacaa cccgacgcgt atcatcgcgc cgtatcttca ttttaaacgg aaatacgaat
     2041 catgcgcata actatttata ctcgtaacga ttgcgttcag tgccacgcca ccaaacgggc
     2101 gatggaaaac cggggctttg attttgaaat gattaatgtc gatcgcgttc ctgaagcagc
     2161 ggaagcgttg cgtgctcagg gctttcgtca gttgccggta gtgattgctg gcgatcttag
     2221 ctggtctggt ttccgtccgg acatgattaa ccgtctgcat ccagcgccac acgcggccag
     2281 tgcatgagcc agctcgtcta cttctccagc agctccgaaa acacgcagcg ttttatcgaa
     2341 cgtttaggtc tgcccgcggt gcgcatcccg ctcaatgagc gggaacggat tcaggtagac
     2401 gagccttaca tcctgatcgt gccctcttac ggcggcggcg gtacggctgg cgcggtgcca
     2461 cgacaggtaa ttcgcttttt aaacgacgag cacaaccggg cgttgcttcg cggcgttatt
     2521 gcttctggta atcgcaactt tggtgaggcg tatggccgcg ccggagatgt gattgcccgg
     2581 aaatgcggcg tgccgtggct gtaccgtttt gaactcatgg gtacgcaaag cgatatcgaa
     2641 aacgttcgta aaggagtaac cgaattttgg caacgacaac cgcagaatgc ctgacgcagg
     2701 aaacgatgga ttaccacgcg ctgaatgcga tgcttaatct ttacgatagc gcaggtcgca
     2761 ttcagttcga taaagaccgc caggccgttg acgcctttat tgcgacgcat gtgcgtccga
     2821 acagtgtgac cttcagtagc cagcagcagc gcctgaactg gctggtcaac gaaggttact
     2881 atgatgaaag cgttcttaat cgctactctc gcgactttgt cattacgctg tttgcccacg
     2941 cacacaccag cggttttcgt ttccagacat tcctcggggc atggaagttt tacaccagct
     3001 atacgttgaa gacattcgac ggtaaacgtt atctggaaga ttttgccgat cgagtcacga
     3061 tggtggcgct gacgctggca caaggcgatg agacgctggc gttgcaactg accgatgaaa
     3121 tgctgtcagg acgctttcag ccagccacgc caacattcct caactgcggt aagcagcagc
     3181 gcggcgaact ggtttcctgt tttttgctgc gtattgaaga caatatggag tcgattggtc
     3241 gggcggtaaa ttccgcactg cagctgtcga aacgcggcgg cggcgtagca tttttgctgt
     3301 cgaatctgcg ggaagcgggc gcgccaatta aacgtattga aaatcaatct tctggcgtaa
     3361 ttccggtgat gaaaatgttg gaagacgcat tttcctatgc caaccaactc ggcgcgcgtc
     3421 agggggcagg tgcagtctat ttacatgctc atcatcccga tattctgcgt tttctcgaca
     3481 cgaaacggga aaatgccgac gaaaaaatcc gcattaaaac actgtcgctg ggcgtggtga
     3541 tcccggatat cactttccat ctggcaaaag agaatgcgca gatggcgctg ttttcgccct
     3601 atgacgttga gcgagtttat ggcaagccgt ttgccgatat cgccattagc gaacactatg
     3661 acgaactggt tgccgatgaa cgcatccgca aaaaatacct caacgcccgt gatttcttcc
     3721 agcgactggc agaaatccag tttgagtccg gctatcccta catcatgtat gaagacacag
     3781 taaaccgtgc taaccctatc gccgggcgca taaatatgag taatctctgc tcagaaattt
     3841 tgcaggttaa cagcgcctca gagtatgacg agaatctcga ctatgcccgc acaggccatg
     3901 atatttcctg caatttaggt tcgttgaata ttgcgcacac catggattcc cccgattttt
     3961 cccgcacggt agagactgcc gtgcgcggtt taacggcggt gtcagatatg agtcatatcc
     4021 gcagcgtgcc gtccatcgaa gccggaaatg ccgcctcgca cgccatcgga ctggggcaga
     4081 tgaatttaca tggttatctg gcgagggaag gcatcgctta tggttcgccg gaagccctgg
     4141 atttcaccaa tctctatttc tataccatca cctggcacgc actgcgtacc tcgatgttgc
     4201 tggcgcgcga acgcggtgaa accttcgccg ggttcaaaca gtcacgctat gccagtggtg
     4261 aatatttcag ccaatatctg caaggtaact ggcagccgaa aacggcgaaa gttggcgaac
     4321 tgtttgcccg cagctgtatt acgttaccta cccgtgagat gtgggcgcaa ctgcgcgacg
     4381 acgtgatgcg ctacggcata tacaaccaga atctccaggc ggtgccgcca accgggtcta
     4441 tctcttatat caaccatgct acgtcgagta ttcatccgat tgtggcgaaa gtagagatac
     4501 gcaaagaggg caaaacagga cgcgtttact accctgcccc gtttatgact aacgaaaatc
     4561 tggcgctgta tcaggacgct tacgaaattg gcgcagaaaa gatcatcgac acctacgcgg
     4621 aagcgactcg ccatgtcgat caggggctgt cgctgacgct gtttttcccc gataccgcca
     4681 ccactcgcga tatcaacaaa gcgcagattt acgcctggcg caaaggtatc aaaacgctct
     4741 attacatccg cctgcgtcag atggcgctgg aaggcactga aattgaaggc tgcgtctcct
     4801 gtgcacttta aggaatatct atgaaactct cacgtatcag cgccatcaac tggaacaaga
     4861 tatctgacga taaagatctg gaggtgtgga atcgcctgac cagcaatttc tggctaccag
     4921 aaaaggtgcc gctgtcgaac gatattcccg cctggcagac attaactgtc gtagaacaac
     4981 aactgacgat gcgcgttttt actggcctga cgctgctcga cacgctgcaa aatgttatcg
     5041 gcgcgccttc gctgatgccc gatgcgctca cgcctcatga agaggcggta ttatcgaata
     5101 tcagctttat ggaagcggtt catgcccgct cttacagttc gattttctcg acgctgtgcc
     5161 agaccaaaga tgtcgatgcc gcctacgcct ggagtgaaga aaacgcaccg ttgcagcgaa
     5221 aagctcagat tattcagcaa cattatcgcg gtgatgatcc gctgaaaaag aaaatcgcca
     5281 gtgtgtttct tgaatctttt ttgttctatt ccggtttctg gctgccgatg tatttttcca
     5341 gccgcggaaa gctgaccaat accgcggacc tgatccgtct gattatccgc gatgaagcag
     5401 tccacggtta ctacataggc tataaatatc agaaaaacat ggaaaagata tctctgggac
     5461 aacgtgaaga gttgaagagt ttcgccttcg atttgttgct ggaactctac gacaacgagt
     5521 tgcaatacac cgatgagctg tacgccgaaa ccccgtgggc tgacgatgtg aaagcgtttc
     5581 tctgttacaa caccaataag gctttgatga atctgggcta cgaaccgtta tttcccgcag
     5641 aaatggcgga agtgaatccg gcaatcctcg ccgcgctttc gccgaatgcc gatgaaaatc
     5701 acgatttctt ttccggttca ggctcctctt atgtgatggg gaaagcggtt gaaacagaag
     5761 atgaagactg gaatttctga gggtgttatt ttcaaaaata tcactacccg cagcagggaa
     5821 ataattcccg ccaaatagct ttttatcacg caaatattct gtagtgatct acactgatac
     5881 tctgttgcat tattcgcctg aaactacaat attcaggcgt tttttcgcta tctatgacaa
     5941 aaaatatcaa cttttctcga tttgctctca gcccttatat cacggaaaat tccggcgatt
     6001 tgctcgcatc aatattcatg ccacatttgc cctcaggggt tgcctcagat tctcagtatg
     6061 ttagggtaga aaaaagtgac tattcccatt gggtaatata tcgacataaa caaataaaga
     6121 aatcattcta ttgcatggcc attaaattaa aaattaaaaa tctttataaa atatttggtg
     6181 agcatccaca gcgagcgttc aaatatatcg aacaaggact ttcaaaagaa caaattctgg
     6241 aaaaaactgg gctatcgctt ggcgtaaaag acgccagtct ggccattgaa gaaggcgaga
     6301 tatttgtcat catgggatta tccggctcgg gtaaatccac aatggtacgc cttctcaatc
     6361 gcctgattga acccacccgc gggcaagtgc tgattgatgg tgtggatatt gccaaaatat
     6421 ccgacgccga actccgtgag gtgcgcagaa aaaagattgc gatggtcttc cagtcctttg
     6481 ccttaatgcc gcatatgacc gtgctggaca atactgcgtt cggtatggaa ttggccggaa
     6541 ttaatgccga agaacgccgg gaaaaagccc ttgatgcact gcgtcaggtc gggctggaaa
     6601 attatgccca cagctacccg gatgaactct ctggcgggat gcgtcaacgt gtgggattag
     6661 cccgcgcgtt agcgattaat ccggatatat tattaatgga cgaagccttc tcggcgctcg
     6721 atccattaat tcgcaccgag atgcaggatg agctggtaaa attacaggcg aaacatcagc
     6781 gcaccattgt ctttatttcc cacgatcttg atgaagccat gcgtattggc gaccgaattg
     6841 ccattatgca aaatggtgaa gtggtacagg tcggcacacc ggatgaaatt ctcaataatc
     6901 cggcgaatga ttatgtccgt accttcttcc gtggcgttga tattagtcag gtgttcagtg
     6961 cgaaagatat tgcccgccgg acaccgaatg gcttaattcg taaaactcct ggcttcggcc
     7021 cacgttcggc actgaaatta ttgcaggatg aagatcgcga atatggctac gttatcgaac
     7081 gcggtaataa gtttgtcggc gcagtctcca tcgattcgct taaaaccgcg ttaacgcagc
     7141 agcaaggtct tgatgcggcg ctgattgatg cgccgttagc agtcgatgca caaacgcctc
     7201 ttagcgagtt gctctctcat gtcggacagg caccctgtgc ggtgcccgtg gtcgacgagg
     7261 accaacagta tgtcggcatc atttcgaaag gaatgctgct gcgcgcttta gatcgtgagg
     7321 gggtaaataa tggctgatca aaataatccg tgggatacca cgccagcggc ggacagtgcc
     7381 gcgcaatccg cagacgcctg gggtacaccg acgactgcac cggctgacgg cggtggtgct
     7441 gactggctga ccagtacgcc tgcgccaaac gtcgagcatt ttaatattct cgatccgttc
     7501 cataaaacgc tgatcccgct cgacagttgg gtcactgaag ggatcgactg ggttgttacc
     7561 catttccgtc ccgtcttcca gggcgtgcgc gttccggttg attatatcct caacggtttc
     7621 cagcaattgc tgctgggtat gccagcgcca gtggcgatta tcgttttcgc tctcatcacc
     7681 tggcagattt ccggggtcgg aatgggcgtg gcgacgctgg tctcgctgat tgccatcggc
     7741 gcaatcggtg cctggtcgca ggcaatggtg actctggcgc tggtgttaac tgccctgctg
     7801 ttctgtatcg tcatcggttt gccgttgggg atatggctgg cgagaagtcc gcgagcggcg
     7861 aaaattattc gtccactgct tgatgccatg cagaccacgc cagcgtttgt ttatctggtg
     7921 ccaatcgtca tgctatttgg tatcggtaac gtgccgggcg tggtggtgac gatcatcttt
     7981 gcgctgccgc cgattatccg tctgaccatt ctggggatta accaggttcc ggcggatctg
     8041 attgaagcct cgcgctcatt cggtgccagc ccgcgccaga tgctgttcaa agttcagtta
     8101 ccgctggcga tgccgaccat tatggcgggc gttaaccaga cgctgatgct ggccctttct
     8161 atggtggtca ttgcctcgat gattgccgtc ggcgggctgg gtcagatggt acttcgcggt
     8221 atcggtcgtc tggatatggg gcttgccacc gttggcggcg tcgggattgt gatcctcgcc
     8281 attatcctcg atcgcctgac gcaggccgtt gggcgcgact cacgcagtcg cggcaaccgt
     8341 cgctggtaca ccactggccc tgttggtctg ctgacccgcc cattcattaa gtaactctgc
     8401 acttgcccgg tgacgccggg cattatcacc ctgccaaaaa aaggaataac aatgcgacat
     8461 agcgtacttt ttgcgacagc gtttgccacg cttatctcta cacaaacttt tgctgccgat
     8521 ctgccgggca aaggcattac tgttaatcca gttcagagca ccatcactga agaaaccttc
     8581 cagacgctgc tggtcagtcg tgcgctggag aaattaggtt ataccgtcaa caaacccagc
     8641 gaagtggatt acaacgttgg ctacacctcg cttgcttccg gcgatgcaac cttcaccgcc
     8701 gtgaactgga cgccactcca tgacaacatg tacgaagctg ccggtggcga taagaaattt
     8761 tatcgtgaag gggtatttgt taacggcgca gcacagggtt acctgatcga taagaaaacc
     8821 gccgaccaat acaaaatcac caacatcgca caactgaaag atccgaagat cgccaaactg
     8881 ttcgatacca acggcgacgg taaagcggat ttaaccggtt gtaaccctgg ctggggctgc
     8941 gaaggtgcga tcaaccacca gcttgccgcg tatgaactga ccaacaccgt gacgcataat
     9001 caggggaact acgcagcgat gatggccgac accatcagtc gctacaaaga gggcaaaccg
     9061 gtgttttatt acacctggac gccgtactgg gtgagtaatg agctgaagcc agggaaagat
     9121 gtggtctggt tgcaggtgcc gttctccgca ctgccgggcg ataaaaatgc cgataccaaa
     9181 ctgccgaatg gcgcgaatta cggcttcccg gtcagcacca tgcatatcgt tgccaacaaa
     9241 gcctggaccg agaaaaatcc ggcggcagcc aaactgtttg ccattatgca gttgccagtg
     9301 gccgatatta acgcccagaa cgccattatg catgacggca aagcctcaga aggcgatatt
     9361 cagggccacg ttgatggctg gatcaaagcc caccagcagc agttcgatgg ctgggtgaat
     9421 gaggcgctgg cagcgcagaa gtaattttta ttcgggcgga taaggcgttt tcgcctcatc
     9481 cgccgctctg tacacaatgc ctgatgcgac gctgacgcgt cctatcatgc ctacaaacca
     9541 catcgcacat ttcaacaagc tatctttcat cccatattca tcaacatccg ctattattga
     9601 tttccagctt aatcatcacc tgatgaacaa aaataatgac taaacctaat catgagctta
     9661 gcccggcgct gatcgtgctg atgtctatcg ccaccggtct ggcggtagcc agtaactatt
     9721 acgcccagcc attgctcgac accatcgcgc gtaacttttc cctttccgcc agttcggcag
     9781 gctttattgt taccgccgcg cagttgggct atgccgcagg tctactgttt cttgttcccc
     9841 tcggtgatat gtttgaacgc cgccgcctga ttgtctcgat gaccttactg gcagcgggcg
     9901 gtatgttaat cactgccagc agtcagtcac tggcgatgat gatcctcggt acggcattaa
     9961 ccggtttatt ctcagtcgtg gcacaaattc tggttccgct ggcagcgacg ctggcttcac
    10021 cggataaacg cggcaaagtg gttggcacca ttatgagcgg tctgctgttg gggatattgc
    10081 tggcacgaac cgttgccggg ttactggcaa acctcggcgg ctggcgcacc gtcttttggg
    10141 ttgcttcggt gttaatggca ctgatggcgc tggcattatg gcgtggtctg ccacaaatga
    10201 aatcagaaac ccacctcaac tacccacagt tgttgggttc cgttttcagc atgttcatca
    10261 gcgataagat cctgcgcacc cgcgcgttgc tgggctgcat gacctttgcc aacttcagca
    10321 ttctctggac ctcaatggcc tttttgcttg ccgctccacc ttttaactac agcgatggtg
    10381 acattggtct gtttgggctt gcgggagctg ccggagcgtt gggcgctcgt ccggcgggcg
    10441 gttttgccga caagggcaaa tcgcaccaca actttcggtc tgcttctgct gttactttca
    10501 tggctggcaa tctggtttgg tcacacttcc gtactggcgt tgattatcgg cattctggtg
    10561 ctggacctca ccgtacaggg cgtgcatatc actaaccaga cggtaattta tcgaatacat
    10621 cctgatgcgc gtaatcgcct gaccgcaggt tacatgacca gctactttat tggcggtgcc
    10681 gccggttcgc taatttcagc ctcagcctgg caacatggcg gttgggctgg cgtttgtctg
    10741 gctggcgcga cgattgccct ggttaactta ctggtctggt ggcgaggttt tcatcgtcag
    10801 gaagccgcaa attaagcaat cagcaaccgt tttcgtggct ttacacttat aagggtgtta
    10861 agaagcccat cagtctgata aggttaagat attcattcag tctatttata atattaacaa
    10921 tcgttaagcg tacactctat ggaaagccct actccacagc ctgctcctgg ttcggcgacc
    10981 ttcatggaag gatgcaaaga cagtttaccg attgttatta gttatattcc ggtggccttt
    11041 gcgttcggtc tgaatgcgac ccgtctggga ttctctcctc tcgaaagcgt ttttttctcc
    11101 tgcatcattt atgcaggcgc gagccagttc gtcattaccg cgatgctggc agccgggagt
    11161 agtttgtggg ttgctgcact gagcgtcatg gcaatggatg ttcgccatgt tttgtatggc
    11221 ccgtcactgc gtagccgtat tattcagcgt ctgcaaaaat cgaaaaccgc cctatgggcg
    11281 tttggcctga cggatgaggt ttttgccgcc gccaccgcaa aactggtacg caataatcgc
    11341 cgctggagcg agaactggat gatcggcatt gccttcagtt catggtcatc gtgggtattt
    11401 ggtacggtaa taggggcatt ctccggcagc ggcttgctgc aaggttatcc cgccgttgaa
    11461 gctgcattag gttttatgct tccggcactc tttatgagtt tcctgctcgc ctctttccag
    11521 cgcaaacaat ctctttgcgt taccgcagct ttagttggtg cccttgcagg cgtaacgcta
    11581 ttttctattc ccgtcgccat tctggcaggc attgtctgtg gctgcctcac tgcgttaatc
    11641 caggcattct ggcaaggagc gcccgatgag ctatgaggtt ctgctgcttg ggttactggt
    11701 tggcgcggcg aattattgct tccgctattt gccgctgcgc ctgcgtgtgg gtaatgcccg
    11761 cccaaccaaa cgtggcgcgg taggtatttt gctcgacacc attggcatcg cctcgatatg
    11821 cgctctgctg gttgtctcta ccgcaccaga agtgatgcac gatacacgcc gtttcgtgcc
    11881 cacgctggtc ggcttcgcgg tactgggtgc cagtttctat aaaacacgca gcattatcat
    11941 cccaacactg cttagtgcgc tggcctatgg gctcgcctgg aaagtgatgg cgattatata
    12001 acccacaaga atcatttttc taaaacaata catttacttt atttgtcact gtcgttacta
    12061 tatcggctga aattaatgag gtcataccca aatggatagt tcgtttacgc ccattgaaca
    12121 aatgctaaaa tttcgcgcca gccgccacga agattttcct tatcaggaga tccttctgac
    12181 tcgtctttgc atgcacatgc aaagcaagct gctggagaac cgcaataaaa tgctgaaggc
    12241 tcaggggatt aacgagacgt tgtttatggc gttgattacg ctggagtctc aggaaaacca
    12301 cagtattcag ccttctgaat taagttgtgc tcttggatca tcccgtacca acgcgacgcg
    12361 tattgccgat gaactggaaa aacgcggttg gatcgaacgt cgtaaaagcg ataacgatcg
    12421 ccgctgcctg catctgcaat taacggaaaa aggtcacgag tttttgcgcg aggttttacc
    12481 accgcagcat aactgcctgc atcaactctg gtccgcgctc agcacaacag aaaaagatca
    12541 gctcgagcaa atcacccgca aattgctctc ccgtctcgac cagatggaac aagacggtgt
    12601 ggttctcgaa gcgatgagct aacgcgtcat ctcgctcaaa aatccagatt tataaaagaa
    12661 aaaatgaccg gccagcatcg caacatgctg gcctttttgg caagcaggtc ggctcagccg
    12721 atgagttaag aagatcgtgg agaacaatat gagcgcaaat gcggagactc aaaccccgca
    12781 gcaaccggta aagaagagcg gcaaacgtaa gcgtctgctc ctccttctca ccttgctctt
    12841 tataattatt gccgtagcga tagggattta ttggtttttg gtactgcgtc atttcgaaga
    12901 aaccgatgac gcatacgtgg cagggaatca gattcaaatt atgtctcagg tgtctggcag
    12961 cgtgacgaaa gtctgggccg ataacaccga ttttgtaaaa gaaggcgatg ttctggtcac
    13021 tctcgacccg acagatgctc gccaggcgtt tgaaaaagcc aaaactgcac tggcttccag
    13081 cgttcgccaa acccaccagc tgatgattaa cagcaaacag ttgcaggcga atattgaggt
    13141 gcaaaaaatc gccctcgcga aagcacaaag cgactacaac cgccgtgtgc cgctgggcaa
    13201 tgccaacctg attggtcgcg aagagctgca acacgcccgc gacgccgtca ccagtgccca
    13261 ggcgcaactg gacgtcgcta ttcaacaata caatgccaat caggcgatga ttctggggac
    13321 taaactggaa gatcagcctg ccgtgcaaca ggctgccacc gaagtacgta acgcctagct
    13381 ggcgctggag cgtacccgta ttgtcagtcc gatgaccggt tatgtttccc gtcgtgccgt
    13441 gcaaccgggt gcgcaaatta gcccaacgac gccgctgatg gcggtcgttc cagccaccaa
    13501 tatgtgggtg gatgccaact ttaaagagac gcagattgcc aatatgcgca tcggtcagcc
    13561 ggtcaccatc accaccgata tttacggcga tgatgtgaaa tacaccggta aagtggttgg
    13621 tctggatatg ggcacaggta gcgcgttctc actgcttccg gcgcaaaatg cgaccggtaa
    13681 ctggatcaaa gtcgttcagc gtctacctgt gcgtatcgaa ctggaccaga aacagctgga
    13741 gcaatacccg ctgcgcattg gtttgtccac gctggtgagc gtcaatacca ctaaccgtga
    13801 cggtcaggta ctggcaaata aagtacgttc aacaccggtc gcggtaagta ccgcgcgtga
    13861 aatcagcctg gcacctgtca ataaactgat cgacgatatc gtaaaagcta acgctggcta
    13921 atccagaggt gcgtgtgatg caacagcaaa aaccgctgga aggcgcgcaa ctggtcatta
    13981 tgacgattgc gctgtcactg gcgacattca tgcaggtgct gaactccacc attgctaacg
    14041 tggcgatccc cactatcgcc gggaatctgg gctcatcgct cagccaagga acgtgggtaa
    14101 tcacttcttt cggggtggcg aatgccatct cgatcccgct taccggctgg ctggcaaagc
    14161 gcgtcgggga agtgaaactg ttcctttggt ccaccatcgc ctttgctatt gcgtcttggg
    14221 cgtgtggtgt ctccagcagc ctgaatatgc tgatcttctt ccgcgtgatt caggggattg
    14281 tcgccgggcc gttgatcccg ctttcgcaaa gtctattgct gaataactac ccgccagcca
    14341 aacgctcgat cgcgctggcg ttgtggtcga tgacggtgat tgtcgcgcca atttgcggcc
    14401 cgatcctcgg cggttatatc agcgataatt accactgggg ctggatattc ttcatcaacg
    14461 tgccgattgg cgtggcggtg gtgttgatga cactgcaaac cctgcgcgga cgtgaaaccc
    14521 gcaccgaacg gcggcggatt gatgccgtgg ggctggcact gctggttatt ggtatcggca
    14581 gcctgcagat tatgctcgac cgcggtaaag agctggactg tttttcatca caggaaatta
    14641 tcattcttac cgtggtggcg gtggtggcta tctgcttcct gattgtctgg gagctgaccg
    14701 acgataaccc gatagtcgat ctgtcgttgt ttaagtcgcg caacttcacc atcggctgct
    14761 tgtgtatcag cctcgcgtat atgctctact tcggcgctat tgttctgctg ccgcagttgt
    14821 tgcaggaggt ctacggttac acggcgacct gggcaggttt ggcctctgcg ccggtaggga
    14881 ttattccggt gattctgtcg ccgattatcg gccgcttcgc gcataaactg gatatgcggc
    14941 ggctggtaac cttcagcttt attatgtatg ccgtctgctt ctactggcgt gcctatacct
    15001 ttgaaccagg tatggatttt tgcgcgtcgg cctggccgca gtttatccag gggtttgcgg
    15061 tggtctgctt ctttatgccg ctgaccacca ttacgctgtc tggtttgcca ccggaacgac
    15121 tggcggcggc atcgagcctc tctaacttta cgcgaacgct ggcggggtct atcggcacgt
    15181 cgataaccac gaccatgtgg accaaccgcg agtcgatgca ccatgcgcag ttgactgagt
    15241 cggtaaaccc gttcaacccg aatgcccagg cgatgtacag tcaactggaa gggcttggga
    15301 tgacgcaaca gcaggcatca ggctggattg cccagcagat caccaatcag gggctgatta
    15361 tttccgccaa tgagatcttc tggatgtcag ccgggatatt cctcgtactg ctggggctgg
    15421 tgtggtttgc taaaccgcca tttggcgcag gtggcggcgg aggcggtgcg cactaagtac
    15481 aactaagcca gttcatttga actggctttt ttcaattaat tgtgaagata gtttactgac
    15541 tagatgtgca gttcctgcaa cttctctttc ggcagtgcca gttcttcgtt gctgttgatg
    15601 cgtacctcac gttccagaat gctacgcgca atatcctgcg cttcctgcaa cgagtgcatc
    15661 tggtaagtgc cacactggta gacgttcagt tccgggatct gattctgatc ctgcactttc
    15721 agcacgtctt ccattgccgc tttccaggca tcagcaacac gctgctcatc tggcgtacca
    15781 atcagactca tataaaaacc ggtgcggcag cccattggcg agatatcgat aatctctaca
    15841 ccattaccgt taagatggtt acgcataaaa ccagcaaaca ggtgctccag ggtatggatc
    15901 cctctttctg gcatcacttc tttgttcggc acgcagaagc gcagatcgaa cacggtgatt
    15961 gcgtcgccat gcggggtgtt cattgttttc gccacccgaa ctgcaggcgc ttccatccgg
    16021 gtatgatcga ctgtgaagct atctaacaac ggcatttagc cacctccggt aattttttta
    16081 aaaattttct gaactctttc ttcccaggcg agtctgagta tatgaaagac gcgcatttgt
    16141 tatcatcatc cctgaattca gagatgaaat tttggccact cccgagtggc ctttttcttt
    16201 tctgtcaggc gtgtttttcc agccacaccg caaacggttc ggtatcagcg gtttccattt
    16261 cctgctgacg gcgttcagat gcctcgcgct cggctacaaa atcctcttcg cgcagaattt
    16321 ccagcggctc ttcacgcagc agattacggt aggcttctgc aaacgctttg cctgttccgc
    16381 caataccagt atcaatcata gaccttaaga tacgggcaga gaaagtcaga tcgggattat
    16441 cgaagcaggc aaccagttca tcacacactt tctgatacgc ttcgccgccg ttaatgctat
    16501 ccagcgtttg cgcgacgcgt ttcagatcgc ggaacagatc tttacccacc tgcagtaacg
    16561 ggaactgcgc ggtttcgcag ccgataccca gcgtcaggcc cggtttgcga ccttcaagga
    16621 tcacccggtt ccagttaacg cgcgtacagg caagttcgct actgctcatt tccggtgcat
    16681 cagccagcgc acaccagacc ataaacaggt cgaggaatcg cacctgctgt tcatctacac
    16741 caatcggcga gaacgggttg atgtccagcg aacgcacttc aatatattca atgccgccac
    16801 gtaacagcgc atcagaaggt gactcgccgc tgcgggtaac gcgttttgga cgaatcggcg
    16861 catacagttc gttttcaatc tgcagcacgt tgctattgat ttgcagcctc ttaccgtctt
    16921 tctcaatacc aatcttcgcg tactcttccg atggcgtttt gattgcctgt ttaaggcccg
    16981 ctacgtactc gtaaagatcg ttgaaggtaa taccaagatt gctttgcgat ttattggtat
    17041 agccgagatc gctcaaacga agagaggtcg catacggcag gtaatacata ccgcactcgg
    17101 ttttctcaaa cggcagcgac gttggttttc cttgcaggaa agaagaacaa atcgccggag
    17161 atgcaccaaa cagataagga ataacccaac cgaaacgata gtaattgcgg ataacgcgga
    17221 aatagcccgc agaaattttc tctttggcat cagcgcccga gatatcaccg cacttcgctt
    17281 gccagaatgc cattggcaaa gagaaattgt agtgcacgcc ggaaatggtt tgcatcagcg
    17341 cgccgtagcg atttttcagc ccttcacgat acagcgtttt aaagcgtccg gtgttagaag
    17401 tgccgtactg tgccagttcg atgtcctgac cttctgcgat gtagcatggc atacttaacg
    17461 gccacatccg ctcatcgccc atattgcgcg ccgtataacg atgcagatcg cgcataaagg
    17521 tcagcatatg ttcaatatca ccatccactg gtgtaatgaa ttccagcaat gcttccgcaa
    17581 aatcggtagt aatccatttg tgcgtcagtg cggaacctaa tgcttcagga tgacctgttg
    17641 ttgccagtgt gccatcagca ttaacacgca aagtttcgcg ctccagccca cgctgtatcc
    17701 cctttaacgc ctgaggatgt ttttccagcc aggccagcgc ctgtgatacg tccgggatca
    17761 aattgacctc ccgcctgtca aaatcgtttt aattagcata actgtaatgg tgaccatatg
    17821 tgcaggccta caattagtgc caccacatca tgccctgaac ggtcgctgct gcaactgcaa
    17881 catagcgtaa cgctttacca aggcacaaaa aaaagattac tggcccccag gagatgcgca
    17941 tccatcccgc taacagacac agtaaatcgc caaccacggg catccagctt aataatagcg
    18001 tgactgcacc atagcgtttc agccagccgg tagctttctc ttgccagcgc gatgttttac
    18061 gcaatggaaa gaaacgccca aggataacgt tagttaaccc tccaaggcta ttacccattg
    18121 ttgctgttaa aactaaaacc cagggatgac tgatcccgga aagcaacatt gccaccagca
    18181 cgacttcgga gttgccgggt aatagtgtag cgctgagaaa actactggca aacaacgaga
    18241 aaagcgataa cgcttcactc acagcaagcg aacatccaca gcgtccatgc ctgctgcacg
    18301 ggccgcctga ataccgaaat cggcatcttc aaagaccaca cactgcgtcg gttgcacgcc
    18361 catacgctgc gcgcacaaca aaaatgtgtc tggcgcgggt ttatggtgtt tgacgtgatc
    18421 agcagcgacg acggcgtcaa aataacggcg taatcccagg tgcgccagca atgcctcagc
    18481 gatggcgctt tcactccccg ttcctacagc cattgggcga cgaccatgcc aacttttcac
    18541 cacttcaaca agaggaagcg gctcgacact atccagcagc atacttctta ctgcttctgt
    18601 tttttcacgc gctaacgcat gcgggtcgag atcggcctga ttcagctcaa taattgcctg
    18661 agcaatacgc caggtgggcg atccattaag cgcaatcatc gcctgaatat cgtactgaag
    18721 accgtagtgc cctaatactt cgcgccacgc tttacggtgc gtaggcgccg tatccaggat
    18781 tgtgccatcc atatcaaaaa ttaaacctgc ataacgctcg tacatggtct tctcgcaagt
    18841 cgaaaaatat gacgctactt tagcgtaatt gcttgatttt gtcgctgatg gcgaaggaga
    18901 agaaaattca ggaaatggat aaagtagtta tttagaaatg agatattttt gaaggaaatt
    18961 ttttggagaa tatggtgcat ccgggaggat tcgaacctcc gaccgctcgg ttcgtagccg
    19021 agtactctat ccagctg
//
LOCUS       DAZSIO010000071        18855 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZSIO010000071 DAZSIO010000000
VERSION     DAZSIO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18855
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.071"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHX5T_002259"
     CDS             <1..122
                     /locus_tag="ACHX5T_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR8467442.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
     gene            138..686
                     /gene="wcaF"
                     /locus_tag="ACHX5T_002260"
     CDS             138..686
                     /gene="wcaF"
                     /locus_tag="ACHX5T_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR8467443.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            712..1833
                     /gene="gmd"
                     /locus_tag="ACHX5T_002261"
     CDS             712..1833
                     /gene="gmd"
                     /locus_tag="ACHX5T_002261"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR8467444.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            1836..2804
                     /gene="fcl"
                     /locus_tag="ACHX5T_002262"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             1836..2804
                     /gene="fcl"
                     /locus_tag="ACHX5T_002262"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR8467445.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            2804..3283
                     /gene="gmm"
                     /locus_tag="ACHX5T_002263"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             2804..3283
                     /gene="gmm"
                     /locus_tag="ACHX5T_002263"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR8467446.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            3280..4503
                     /gene="wcaI"
                     /locus_tag="ACHX5T_002264"
     CDS             3280..4503
                     /gene="wcaI"
                     /locus_tag="ACHX5T_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR8467447.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            4506..5942
                     /gene="cpsB"
                     /locus_tag="ACHX5T_002265"
     CDS             4506..5942
                     /gene="cpsB"
                     /locus_tag="ACHX5T_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR8467448.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            6135..7512
                     /gene="cpsG"
                     /locus_tag="ACHX5T_002266"
                     /gene_synonym="manB"
                     /pseudo
     CDS             6135..7512
                     /gene="cpsG"
                     /locus_tag="ACHX5T_002266"
                     /gene_synonym="manB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
     gene            7567..8961
                     /gene="wcaJ"
                     /locus_tag="ACHX5T_002267"
     CDS             7567..8961
                     /gene="wcaJ"
                     /locus_tag="ACHX5T_002267"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFR8467449.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            8963..10441
                     /gene="wzxC"
                     /locus_tag="ACHX5T_002268"
     CDS             8963..10441
                     /gene="wzxC"
                     /locus_tag="ACHX5T_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFR8467450.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            10621..11901
                     /gene="wcaK"
                     /locus_tag="ACHX5T_002269"
     CDS             10621..11901
                     /gene="wcaK"
                     /locus_tag="ACHX5T_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFR8467451.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            11898..13118
                     /gene="wcaL"
                     /locus_tag="ACHX5T_002270"
     CDS             11898..13118
                     /gene="wcaL"
                     /locus_tag="ACHX5T_002270"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFR8467452.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            13129..14523
                     /gene="wcaM"
                     /locus_tag="ACHX5T_002271"
     CDS             13129..14523
                     /gene="wcaM"
                     /locus_tag="ACHX5T_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFR8467453.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            14698..15591
                     /gene="galF"
                     /locus_tag="ACHX5T_002272"
     CDS             14698..15591
                     /gene="galF"
                     /locus_tag="ACHX5T_002272"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFR8467454.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            15964..17049
                     /gene="rfbB"
                     /locus_tag="ACHX5T_002273"
     CDS             15964..17049
                     /gene="rfbB"
                     /locus_tag="ACHX5T_002273"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR8467455.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            17049..17948
                     /gene="rfbD"
                     /locus_tag="ACHX5T_002274"
     CDS             17049..17948
                     /gene="rfbD"
                     /locus_tag="ACHX5T_002274"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFR8467456.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            18007..>18855
                     /gene="rfbA"
                     /locus_tag="ACHX5T_002275"
     CDS             18007..>18855
                     /gene="rfbA"
                     /locus_tag="ACHX5T_002275"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR8467457.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQ"
BASE COUNT         4658 a         4618 c         5155 g         4424 t
ORIGIN      
        1 gtgctgacgc caaaaaagtc caacggcaga tattacatgt acctggcttc tgggcagaat
       61 tatcctggca tttacgccaa cgtacgacag gtaagactaa agccttatat aacaaagtct
      121 gaatataagg aaaaccaatg caagatttaa gcggtttctc ggtgccgaaa gggttccggg
      181 gcggcaacgc tattaaagtg caattatggt gggcagtaca ggcaacaata tttgcctggt
      241 cgccacaagt attgtatcgc tggcgggctt ttttattacg tttattcgga gcaaaaatag
      301 gaaaaaacgt agttattcgt ccgtcagtaa aaattaccta tccgtggaaa ttaaccttag
      361 gtgattacgc gtgggttggc gatgacgtca atttatatac cctcggtgaa ataaccattg
      421 gcgcacattc ggtgatatcg caaaaaagtt atttatgcac cggtagccac gaccatgcaa
      481 gtcaacattt caccattaac gccacgccta ttgtgattgg cgagaaatgc tggctggcaa
      541 ccgatgtctt tgttgcccca ggcgtcacaa tcggcgacgg caccgtcgtg ggtgcacgaa
      601 gcagtgtttt taaatcgctt ccggcaaatg tggtttgccg ggggaatccc gcagtggtga
      661 tacgcgaacg cgttgaaact gaataaattc aaaaatacag aggaataaga catgtcaaaa
      721 gtcgctctca tcaccggtgt aaccggacaa gacggttctt acctggcaga gtttctgctg
      781 gaaaaaggtt acgaggtgca tggtattaag cgtcgcgcat cgtcattcaa caccgagcgc
      841 gtggatcaca tttatcagga tccgcacacc tgcaacccga aattccatct gcattatggc
      901 gacctgagtg atacctctaa cctgacgcgc attttgcgtg aagtacagcc ggatgaagtg
      961 tacaacctag gcgcaatgag ccacgttgcg gtctcttttg agtcaccaga atataccgct
     1021 gacgtcgacg cgatgggtac gctgcgcctg ctggaggcga tccgcttcct cggtctggaa
     1081 aagaaaactc gtttctatca ggcttccacc tctgaactgt atggtctggt gcaggaaatt
     1141 ccgcagaaag aaaccacgcc gttctacccg cgatctccgt atgcggtcgc caaactgtac
     1201 gcctactgga tcaccgttaa ctaccgcgaa tcctacggca tgtacgcttg taacggaatt
     1261 ctcttcaacc atgaatcccc gcgccgcggc gaaaccttcg ttacccgcaa aatcacccgc
     1321 gcaatcgcca acatcgccca ggggctggag tcgtgcctgt acctcggcaa tatggattcc
     1381 ctgcgtgact ggggccacgc caaagactac gtaaaaatgc agtggatgat gctgcaacag
     1441 gaacagccgg aagatttcgt tatcgcgacc ggcgttcagt actccgtacg tcagtttgtg
     1501 gaaatggcgg cagcacagct gggcatcaaa ctgcgctttg aaggcacggg tgttgaagag
     1561 aagggcattg tggtttccgt caccgggcat gacgcgccgg gcgttaaacc gggtgatgtg
     1621 attatcgccg ttgatccgcg ttacttccgt ccggcagaag ttgaaacgct gctcggcgac
     1681 ccgaccaaag cgcacgaaaa actgggctgg aaaccggaaa tcaccctcag agagatggtg
     1741 tctgaaatgg tggctaatga cctcgaagcg gcgaaaaaac actctctgct gaaatctcac
     1801 ggctacgacg tggcgatcgc gctggagtca taagcatgag taaacaacgc atttttatcg
     1861 ctggccattg tgggatggtc ggttctgcca tcaagcggca gctcgaacag cgcggtgatg
     1921 tggagctggt attacgtacc cgcgatgaac tgaacctgct ggacagccgc gccgtgcatg
     1981 atttctttgc cagcgaacgc attgaccagg tctatctggc ggcggcgaaa gtgggcggca
     2041 ttgttgccaa caacacctat ccggcggatt tcatctacca gaacatgatg attgagagca
     2101 acatcattca cgccgcgcat cagaacgacg tgaacaaact gatgtttctc ggatcgtcct
     2161 gcatctaccc gaaactggca aaacagccga tggcagaaag cgagttattg cagggcacgc
     2221 tggagccgac caacgagcct tacgccattg ccaaaatcgc cgggatcaaa ctgtgcgaat
     2281 cttacaaccg ccagtatgga cgcgattacc gcactgtcat gccgaccaac ctgtacgggc
     2341 cgcatgacaa cttccacccg agtaactccc atgtgatacc agcattgctg cgtcgcttcc
     2401 acgaggcgac ggcacagaat gcgccggacg tggtggtatg gggcagcggt acaccgatgc
     2461 gtgaattcct gcacgtcgat gatatggcgg cggcgagcat tcatgtcatg gagctggcgc
     2521 atgaagtctg gctggagaat acccaaccga tgctgtcgca cattaacgtc ggcacgggcg
     2581 ttgactgcac tatccgcgag ctggcgcaaa ccatcgccaa agtggtgggt tacaaaggtc
     2641 gggtggtttt tgatgccagc aaaccggatg gtacgccgcg caaactgctg gatgtgacgc
     2701 gcctgcatca gcttggctgg tatcacgaaa tctcactgga agcggggctt gccagcactt
     2761 accagtggtt ccttgagaat caagaccgct ttcgggggga atgatgtttt tacgtcagga
     2821 agactttgcc actgtagtgc gctccacacc gcttgtctct ctcgacttta ttgtcgagaa
     2881 cagtcgcggc gagtttctgc ttggcaaaag aaccaaccgc ccggcgcagg gttactggtt
     2941 tgtgccggga gggcgcgtgc agaaagacga aacgctggaa gccgcatttg agcggctgac
     3001 gatggcggaa ctggggctgc gtctgccgat aacagcaggc cagttttacg gtgtctggca
     3061 gcatttttat gacgataact tctccggcac ggatttcacc actcactatg tggtgctcgg
     3121 ttttcgcttc agagtagcgg aagaagagct gttactgccg gatgagcagc atgacgatta
     3181 ccgctggctg acgccggacg cgctgctcgc cagtaacgat gttcacgcta acagccgagc
     3241 ctattttctc gctgagaagc gtgccggagt acccggatta tgaaaatact ggtctacggc
     3301 attaactact cgccggagtt aaccggcatc ggcaaataca ccggcgagat ggtggaatgg
     3361 ttggcggcac aaggtcatga ggtgcgggtc attaccgcac cgccttacta cccgcaatgg
     3421 caggtgggtg agaactattc cgcctggcgc tacaaacgag aagaaggggc cgccacggtg
     3481 tggcgctgcc cgctgtacgt gccaaaacag ccgagcaccc tgaaacgctt gttgcatctc
     3541 ggcagttttg ccgtcagcag tttctttcca ctgatggcgc aacgtcgctg gaagccggat
     3601 cgcattattg gcgtagtgcc aacgctgttt tgcacgccgg gaatgcgcct gctggcgaag
     3661 ctctctggtg cgcgtaccgt gctgcatatt caggattacg aagtggacgc catgctgggg
     3721 ctgggccttg ccggaaaagg caaaggcggc aaagtggcac agctggcaac ggcgttcgaa
     3781 cgtagcggac tgcataacgt cgataacgtt tccacgattt cgcgttcgat gatgaataaa
     3841 gccatcgaaa aaggcgtggc ggcggataac gtcatcttct tccccaactg gtcggaaatt
     3901 gcccgttttc agcatgttgc agacgccgat gttgatgccc ttcgtaacca gcttggcctg
     3961 ccggataaca aaaaaatcat tctttactcc ggcaatattg gtgaaaagca ggggctggaa
     4021 aacgttattg aagctgccga tctcctgcgc gatgaaccgc tgatttttgc cattgtcggg
     4081 cagggcggcg gcaaagcgcg gctggaaaaa atggcgcagc agcgtggact gcgcaacatg
     4141 caatttttcc cgctgcaatc gtatgacgct ttacctgcac tgctgaagat gggcgattgc
     4201 catctggtgg tgcaaaaacg cggcgcggca gatgccgtat tgccgtcgaa actgaccaat
     4261 attctggcgg taggcggtaa cgcggtgatt accgcagagg cccacacaga actggggcag
     4321 ctttgcgaaa cctttccggg tattgcggtt tgcgtagaac cggaatcggt cgaggcgctg
     4381 gtggcgggga ttcgtcaggc gctcctgctg cccaaacata acacggtggc acgtgaatat
     4441 gccgaacgca cgctcgataa agagaacgtg ttacgtcaat ttataaatga tattcgggga
     4501 taattatggc gcagtcgaaa ctctatccag ttgtgatggc aggtggctcc ggtagccgtt
     4561 tatggccgct ttcccgcgta ctttacccca agcagttttt atgcctgaaa ggcgatctca
     4621 ccatgctgca aaccaccatc tgccgcctga acggcgtgga gtgcgaaagc ccggtggtga
     4681 tttgcaatga gcagcaccgc tttattgtcg cggaacagct gcgtcaactg aacaaactca
     4741 ccgagaacat tattctcgaa ccggcagggc gtaacactgc acctgccatt gcactggcgg
     4801 cgctggcggc aaaacgtcat agcccggaga gcgacccgtt aatgctggta ttggcggcgg
     4861 atcatgtgat tgccgatgaa gacgcgttcc gtgccgccgt gcgtaatgcc atgccttatg
     4921 ccgaagcggg caagctggtg acctttggca ttgtgccgga tctaccagaa accggttatg
     4981 gctatattcg tcgcggtgaa gtgtctgtgg gtgagcagga tacggtggcc tttgaagtgg
     5041 cgcagtttgt cgaaaaaccg aatctggaaa ccgcccaggc ctatgtagca agcggcgaat
     5101 attactggaa cagcggcatg ttcctgttcc gcgccggacg ctatctcgaa gaactgaaaa
     5161 aatatcgccc ggatatcctc gatgcctgcg aaaaagcgat gagcgccgtc gatccggatc
     5221 tcgattttat tcgtgtggat gaagaagcgt ttctcgcctg cccggaagag tcggtggatt
     5281 acgcggtcat ggaacctacg gcagatgctg ttgtggtgcc gatggatgcg ggctggagcg
     5341 atgttggttc ctggtcttca ttatgggaga tcagcgccca caccgccgag ggcaacgttt
     5401 gccacggcga tgtgattaat cacaaaactg aaaacagcta tgtgtatgcc gaatcaggcc
     5461 tggtcaccac cgtcggggtg aaagatctgg tagtggtgca gaccaaagat gcggtgctga
     5521 ttgccgaccg taacgcggta caggatgtga aaaaagtggt cgagcagatc aaagccgatg
     5581 gtcgccatga gcatcgggtg catcgcgaag tgtatcgtcc gtggggcaaa tatgactcta
     5641 tcgacgcggg cgaccgctac caggtgaaac gcatcaccgt gaaaccgggc gagggtttgt
     5701 cggtacagat gcaccatcac cgcgcggaac actgggtagt ggtcgcggga acggcaaaag
     5761 tcaccattga tggtgatatc aaactgcttg gtgaaaacga gtccatttat attccgctgg
     5821 gggcgacgca ctgcctggaa aacccgggga aaattccgct cgatttaatt gaagtgcgct
     5881 ccggctctta tctcgaagag gatgatgtgg tgcgcttcgc ggatcgctac ggacgggtgt
     5941 aaacgtcgca tcaggcgctg tttgtcggat gcggcgtgaa cgccttatcc gacctacggt
     6001 tcggttttgt aggcctgata agacgcggca gcgtcgcatc aggcaatgaa tgcgaaaccg
     6061 cggtgtaaat aacgacaaaa ataaaattgg ccgtttcggt cagggccaac tattgcctga
     6121 aaaagggtaa cgacatgaaa aaattaacct gctttaaagc ctatgatatt cgcgggaaat
     6181 taggcgaaga actgaatgaa gatatcgcct ggcgcattgg tcgcgcctat ggcgaatttc
     6241 tcaaaccgaa aaccattgtg ttaggcggtg atgtccgtct caccagcgaa accttaaaac
     6301 tggcgctggc aaaaggttta caggatgcgg gcgtcgatgt gctggatatc ggtatgtccg
     6361 gcaccgaaga gatctatttc gccacgttcc atctcggcgt ggatggcggc attgaagtta
     6421 ctgccagcca taatccgatg gattacaacg gcatgaagct ggtgcgcgag ggggctcgca
     6481 cgatcagcgg tgataccgga ctgcgcgacg tccagcgtct ggctgaagcc aacgactttc
     6541 ctcccgtcga tgaaaccaaa cgcggtcgct atcagcaaat caacctgcgt gacgcttacg
     6601 ttgatcacct gttcggttat atcaatgtca aaaatctcac gccgctcaag ctggtgatca
     6661 actccgggaa tggcgcagcg ggtccggtgg tggacgccat tgaagcccgc tttaaagccc
     6721 tcggcgcgcc cgtggaatta atcaaagtac acaacacacc ggacggcaat ttccccaacg
     6781 gtattcctaa cccgctgctg ccggaatgtc gcgacgacac ccgcaatgcg gtcatcaaac
     6841 acggcgcgga tatgggcatt gcctttgatg gcgattttga ccgctgtttt ctgtttgacg
     6901 aaaaagggca gtttatcgag ggctactaca ttgtcggcct gctggcagaa gcgttcctcg
     6961 aaaaaaatcc cggcgcgaag atcatccatg atccgcgatc cgcgtctctc ttggaacact
     7021 attgatgtgg tgactgctgc aggcggcacg ccggtaatgt cgaaaaccgg acacgccttt
     7081 attaaagaac ggatgcgcaa ggaagacgcc atctacggtg gcgaaatgag cgcccaccat
     7141 tacttccgtg atttcgctta ctgcgacagc ggcatgatcc cgtggctgct ggtcgccgaa
     7201 ctggtgtgcc tgaaaggaaa aacgctgggc gaactggtgc gcgaccggat ggcggcgttt
     7261 ccggcaagcg gtgagatcaa cagcaaactg gcgcaacccg ttgaggcgat taaccgcgtg
     7321 gaacagcatt ttagccgtga ggcgctggcg gtggatcgca gcgatggcat cagcatgacc
     7381 tttgccgact ggcgctttaa cctgcgcacc tccaataccg aaccggtggt gcgcctgaat
     7441 gtggaatcgc gcggtgatgt gccgctgatg gaagcgcgaa cgcgaactct gctgacgttg
     7501 ctgaacgagt aatgtcggat cttcccttac cccactgcgg gtaaggggct aataacagga
     7561 acaacgatga caaatctaaa aaagcgcgag cgagcgaaaa ccaatgcatc gttaatctct
     7621 atggtgcaac gcttttcaga tatcaccatc atgtttgccg ggctatggct ggtttgcgaa
     7681 gtcagcggac tgtcattcct ctacatgcac ctgttggtgg cgctgattac gctggtggtg
     7741 ttccagatgc tgggcggcat caccgatttt tatcgctcat ggcgcggtgt tcgggcagcg
     7801 acagaatttg ccctgttgct acaaaactgg accttaagcg tgattttcag cgccggactg
     7861 gtggcgttca acaatgattt cgacacgcaa ctgaaaatct ggctggcgtg gtatgggctg
     7921 accagcatcg gactggtggt ttgccgttca tgtattcgca ttggggcggg ctggctgcgt
     7981 aatcatggct acaacaagcg gatggtcgcc gtagcggggg atttagccgc cggacaaatg
     8041 ctgatggaga gtttccgtaa tcagccgtgg ttagggtttg aagtggtggg cgtttaccac
     8101 gacccgaaac cgggcggcgt ttctaacgac tgggcgggta acctgcaaca gctggtcgag
     8161 gatgcaaaag cgggcaagat tcataacgtc tatatcgcga tgcaaatgtg cgacggcgca
     8221 cgagtgaaaa aactggtcca tcaactggcg gataccacct gttcggtgct gctgatcccc
     8281 gacgtcttta ccttcaacat tctccattca cgtctcgaag agatgaacgg cgttccggtg
     8341 gtgccgctgt atgacacgcc gctttcgggg gttaaccgcc tgctaaaacg tgcggaagac
     8401 attgtgctgg cgacgcttat tctgctgctg atctccccgg tgctgtgctg tattgcgctg
     8461 gcggtgaaac tcagttcacc agggccggtt attttccgcc agactcgcta tggtatggat
     8521 ggcaagccga tcaaagtgtg gaagttccgt tccatgaaag tgatggagaa cgacaaagtg
     8581 gtgacccagg cgacgcagaa cgatccgcgc gtcactaaag tggggaactt tctgcgccgc
     8641 acctcgctgg acgaattgcc gcagtttatc aatgtgctga ccggggggat gtcgattgtc
     8701 ggtccacgtc cgcacgcagt agcgcataac gaacagtatc gccagctcat tgaaggctac
     8761 atgctgcgcc ataaagtgaa accgggcatt accggctggg cgcagattaa tggctggcgc
     8821 ggcgaaaccg acacgctgga gaaaatggaa aaacgcgtcg agttcgacct tgagtacatc
     8881 cgcgaatgga gcgtctggtt cgatatcaaa atcgttttcc tgacggtatt caagggcttc
     8941 gttaacaaag cggcatattg atatgagctt acgtgaaaaa accatcagcg gcgcgaagtg
     9001 gtcggctatt gccacggtga tcatcatcgg cctcgggctg gtgcagatga ccgtgctggc
     9061 gcggattatc gacaaccacc agttcggcct gctgactgtg tcgctggtga ttatcgcgct
     9121 ggcagatacg ctttctgact tcggtatcgc taactcgatt attcagcgaa aagaaatcag
     9181 tcaccttgaa ctcaccacgc tgtactggct gaacgtcggg ctggggcttg tggtatgtgt
     9241 ggcggtgttt ttgttgagtg atgtcatcgg cgacgtgctg aataacccgg acctggcacc
     9301 gttgattaaa acattgtcgc tggcgtttgt ggtgatcccc cacgggcaac agttccgcgc
     9361 gttgatgcag aaggagctgg agttcaacaa aatcggcatg atcgaaacca gcgcggtgct
     9421 ggcgggcttc acttttacgg tagttagcgc ccatttctgg ccgctggcga tgaccgcgat
     9481 cctcggttat ctggtcaata gtgcggtgag aacactgctg tttggctact ttggacgcaa
     9541 aatttattgc cccggtctgc acttctcgct ggcgtctgtg gcaccgaact tacgttttgg
     9601 tgcctggctg acggcggaca gcatcatcaa ctatctcaac accaaccttt caacgctggt
     9661 gctggcgcgt attcttggcg cgggcgtggc agggggatac aacctggcgt acaacgtggc
     9721 cgtcgtaccg ccgatgaagc tgaacccaat catcacccgc gtgttgtttc cggcgtttgc
     9781 caaaattcag gacgacaccg aaaagctgcg tgttaacttc tacaagctgc tgtcgatagt
     9841 ggggattatc aactttccgg cgctgctggg gctgatggtg gtgtcgaata acttcgtacc
     9901 gctggtattc ggcgaaaagt ggaacagcat tattccggtg ctgcaattgc tgtgtattgt
     9961 gggtctgctg cgctccgtgg gtaacccgat tggttcgctg ctgatggcga aagcgcgggt
    10021 cgatatcagc tttaaattca acgtattcaa aacatttctg tttattccgg cgattgttat
    10081 tggtgggcag atggcgggcg caatcggcgt cacgctcggt ttcctgctgg tgcagattat
    10141 caacaccatt ctgagttact tcgtgatgat taaaccggtt cttggttcca gttatcgcca
    10201 gtacatcctg agtttgtggc tgccgtttta tctctcgttg ccgacgctgg tggtcagtta
    10261 tgcgctgggc attgtgctga aagggcaact ggcgctgggg atgctgctgg cggtgcaaat
    10321 agccgcgggc gtgctggcgt ttgtggtgat gattgtgctg tcacgccatc cgctggtggt
    10381 ggaagtgaag cgtcagtttt gtcgcagcga aaaaatgaaa atgcttttac gggcggggtg
    10441 aattgctgtt ttcggttccg acaacggttg ccggatgcgg cgtaaaacgc cttatccgtc
    10501 ctacggttct gtgctcggga cctgtaggcc tgataagacg cgccagcgtc gcatcaggca
    10561 aaaaacggca acgaacacaa caaacacagc ctcacctggc atcaaattta tgaggaaaaa
    10621 atgaaattac ttattctggg caaccacact tgcggcaatc gtggcgacag cgccatcttg
    10681 cgcggcttac ttgatgccat caacatcctc aatccacata ccgaagtgga cgtgatgagc
    10741 cgctatccgg tcagttcttc ctggctgctc aaccgcccgg taatgggcga tccgctgttc
    10801 ctgcagatga cacaacacaa tagcgcggcg ggcgttgtcg ggcgcattaa aaaagtcctc
    10861 cgtcgccgct accagcatca ggtattgctc tcacgcgtca ccgacactgg caagctgcgc
    10921 aatatcgcca tcgcccaggg attcaccgac ttcgtgcgcc tgctgtcagg ttacgacgcc
    10981 attattcagg tcggcggatc gttttttgtc gatctctacg gcgtgccgca gtttgaacat
    11041 gcgctttgca catttatggc gaaaaagccg ctgtttatga ttggtcacag cgtcggcccc
    11101 ttccaggatg agcaatttaa ccaactggcg aactatgtct ttggtcactg cgacgcgctg
    11161 atcctgcgcg aatcggtcag ccttgatttg atgaaacgca gaaatatcac caccgcaaaa
    11221 gtggaacatg gcgtcgatac cgcgtggctg gtcgatcacc acaccgaaga cttcaccgcc
    11281 agctatgccg ttcaacactg gctggacgtt gccgcacaac agaaaacagt ggcaattacc
    11341 ctgcgcgaac tggcaccgtt cgacaaacgt ctcggcacca ctcaacaagc gtatgaaaaa
    11401 gcctttgccg gggtggtcaa tcgcattctc gatgaaggct atcaggtgat tgcgctctcc
    11461 acctgtacgg gtatcgacag ctataacaaa gacgaccgca tggtggcgct caatctgcgc
    11521 cagcacatca gcgatcctgc ccgttaccac gtagtgatgg atgaactcaa cgatctggaa
    11581 atgggcaaaa ttctcggagc ctgtgaactc accgtcggta cgcgcctgca ctccgccatt
    11641 atctcaatga actttgctac tccggcgatt gccatcaact atgaacataa atccgccggg
    11701 attatgcagc agctgggact gccagagatg gcgattgata ttcgtcattt actggacggc
    11761 agcctgcaag cgatggttgc ggatacctta ggccagcttc cggtgctgaa cgctcgattt
    11821 aacgaagccg tcagtcgcga acgtcagacg ggaatgcaga tggtgcagtc tgtgcttgag
    11881 cgcatcgggg aggtgaaatg aaggtcggct tcttcttatt gaaattcccg ctgtcgtcgg
    11941 aaaccttcgt cctcaatcaa attaccgcgt ttattgatat gggctttgaa gtggagattg
    12001 tcgctttgca aaaaggcgat acagagaaca cccacgcggc atggacgaaa tacaaccttg
    12061 tcgccagaac ccgctggtta caggacgaac ctgcgggcaa agtagcgaaa ctgcgccacc
    12121 gcgccagcca gaccttacgt ggcattcatc gtaaaaatac ctggcatgcg ctcaacctca
    12181 aacgctatgg tgccgagtcg cggaacctga ttttgtctgc catttgtggt caggtcgcaa
    12241 caccgtttcg cgccgatgtg ttcatcgctc attttggccc ggcgggggta accgcagcaa
    12301 aactacgcga actgggtatc attcgcggca aaattgccac catcttccac ggtattgata
    12361 tctccagtcg ggaagtgctc aaccactaca ctcccgaata tcaacaactg tttcgccgtg
    12421 gcgacctgat gttaccgata agcgatctgt gggccggaag gctgcaaaaa atgggctgcc
    12481 ccagagaaaa aatcgccgta tcgcgtatgg gcgtggacat gacgcgcttt agcccgcgtc
    12541 cggtgaaagc gcccgcaacg ccgctggaaa tcatatctgt cgcacgctta accgagaaaa
    12601 aaggcctgca tgtggcgatt gaagcctgcc gtcagttgaa agagcagggc gtggcatttc
    12661 gctatcgcat cctcggcatt ggcctgtggg aacgacgcct gcgcaccatc atcgaacaat
    12721 atcaactgga agatgtggta gagatgccgg gctttaaacc gagccatgaa gtgaaggaga
    12781 tgctcgacga cgcggatgtc ttcctgttgc catcggtaac gggcgcggat ggcgatatgg
    12841 aaggcattcc ggtggcgcta atggaagcga tggcggtcgg tattccggtg gtttctactc
    12901 tgcatagcgg aattcctgaa ctggtggagg ccgataaatc cggctggctg ctgcctgaga
    12961 acgatgctcg cgcactggca caacgtctgg cggcgtttag ccaactggat accgacgaac
    13021 tggctccggt cgtcaaacgt gcgtgcgaaa aagtcgaaca cgattttaac cagcaggtga
    13081 ttaatcgaga actcgccagc ttgctgcagg ctttatagag gagaacgtat gccatttaaa
    13141 acactctccc gacgcacctt cctgacggca agctcggcgc ttgccttcct ccatacccct
    13201 ttcgctcgcg cacttcccgc ccgacaaagc gttaacatta acgactacaa cccacatgac
    13261 tggatcgcct catttaaaca agccttcagc gaagggcaaa cggtcgtcgt gcctgccgga
    13321 ttcgtttgtg acaatatcaa caccggcatc ttcatccctc ccggcaaaac gttacacatc
    13381 cttggaagcc tgcgcggcaa cggcagaggg cgatttgtct tacaggacgg cagccaggtg
    13441 acagggggag agggcggcgg tatgcataac attaccctgg atgtgcgtgg ttctgactgc
    13501 accatcaaag ggctggcgat gagcggcttt ggcccggtaa tgcagattta tattggcggc
    13561 aaaaacaaac gggtcatgcg caacctgacc atcgataacc tcactgtcag ccacgctaat
    13621 tacgccatct tacgccaggg atttcataac cagattatcg gtgccaacat taccaactgt
    13681 aagttcagcg acttacaggg cgacgccatt gaatggaacg tggcaattaa cgacagtgat
    13741 attttgatat ctgaccatgt catcgagcgt atcaactgta ccaacggcaa aatcaactgg
    13801 ggcatcggca taggccttgc gggaagcact tatgataaca actacccgga agaccaggca
    13861 gtgaaaaact ttgtcgtggc gaatatcacg ggatcggatt gtcggcagtt gatccatgtt
    13921 gaaaatggta aacattttgt tattcgtaat atcaatgccc gcaatatcac gccggatttc
    13981 agtaagaaag cgggcattga taacgcgaca gtcgctattt acggttgtga caatttcgtg
    14041 attgataata ttgaaatgat taatagcgcc gggatgttaa tcggctatgg ggtaattaaa
    14101 ggcaaatatc tctcgatacc gcaaaatttc cgagtgaata atattcaact ggataatacc
    14161 catcttgctt ataaattgcg cggcatccaa atctccgccg ggaatgccgt ctcctttgtg
    14221 tcgctaacta acattgagat gaagcgtgcc tcgctggagt tacacaacaa gccgcaacat
    14281 ctttttatgc gaaatatcaa tgtgatgcag gaatcctcag ttggaccagc attgagcatg
    14341 aacttcgaca tgcgcaaaga cgttcgcggc gtctttatgg cgaaaaaaga aacactgctg
    14401 tctcttgcaa atgttcatgc ggtgaatgaa agagggcaaa gctccgtcga tatcgacagg
    14461 attaatcacc atattgttaa tgtggaaaag attaacttta gattgccgga acggagggag
    14521 tagatttgcg accattcctg gaaaaatgga gccatactta ggaacaatgc tactgcaatc
    14581 cacaacgaag cggcgtaaca tcacaagtaa ttcagtaatc aattcagggt aattgatgct
    14641 ggcgaaaaaa atcgaacaag ctataattca gcaaccattt tacaggtgga tgaaataatg
    14701 acgaatttaa aagcagttat tcctgtagcg ggtctcggga tgcatatgtt gcctgccact
    14761 aaggcgatac ccaaagagat gctaccaatc gtcgacaagc caatgattca gtacattgtt
    14821 gacgagattg tggctgcagg gatcaaagaa atcctcctgg taactcacgc gtccaagaac
    14881 gcggtcgaaa accacttcga cacctcttat gagttagaat cactccttga gcagcgcgtg
    14941 aagcgtcaac tgctggcgga agtacagtcc atctgtccgc cgggcgtgat cattatgaac
    15001 gtgcgtcagg gcgaaccttt aggtttaggc cactccattt tgtgtgcgcg acctgccatt
    15061 ggtgacaacc catttgtcgt ggtactgcca gacgttgtga tcgacgatgc cagcgccgac
    15121 ccgctacgtt acaaccttgc tgccatgatt gcacgtttca acgaaacggg ccgcagccag
    15181 gtgctggcaa aacgtatgcc gggtgacctc tctgaatact ccgtcatcca gactaaagag
    15241 ccgctggacc gtgagggtaa agtcagccgc attgttgaat ttatcgaaaa accggatcag
    15301 ccgcagacgc tggactcaga catcatggcc gtaggtcgct atgtgctttc tgccgatatt
    15361 tggccggaac tggaacgtac tcagcctggt gcatggggac gtattcagct gactgatgct
    15421 attgccgagc tggcgaaaaa acaatccgtt gatgcaatgc tgatgaccgg cgacagttac
    15481 gactgcggca aaaaaatggg ctatatgcag gcgtttgtga agtatggcct acgcaacctg
    15541 aaagaagggg cgaagttccg taaaggtatt gagaagctgt taagcgaata atgaaaatct
    15601 gaccggatgt aacggttgat aagaaaatta taacggcagt gaagattcgt ggcataagtt
    15661 ttgctcattg aattttcctg ccgttgccgt ttttataaag cctataaaaa caagaagtta
    15721 catagctcat attattcaaa tttttccagg attttccttg tttctacggc tgtttggtaa
    15781 gacaattaac gtttgaattt ttcggatttg gcgcgagtgg ctaacgctcg ccacatcgtt
    15841 gagatgcatg cagtgcactg gtagctgtta agccaggggc ggtagcgtgt attaatacct
    15901 ctattaatca aatctggagc agtctatttc acagcatgct ctatagctat atggaataaa
    15961 aaagtgaaga tacttgttac tggtggcgca gggtttattg gttctgctgt tgttcgtcac
    16021 ataataaata atacgcaaga tagtgttgtt aatgtcgata aattaacata cgccgggaac
    16081 ctggaatcac tcgctgatgt ttctgattct gaacgttatg cttttgagca tgcagatatc
    16141 tgcgatgctg tagccatgtc gcgtattttc gcacagcacc agccagacgc ggtgatgcac
    16201 ctggcggcag agagccacgt tgaccgttca ataacgggcc ctgcggcatt tattgaaacc
    16261 aatattgtgg ggacttatgt tcttttagaa gcggcgcgca attactggtc tgctctgaat
    16321 gatgaaaaga aaaaaagttt ccgctttcat catatttcta ctgatgaagt ttatggtgac
    16381 ttaccccatc cggatgaagc aaataataac gaagcgttac cgctatttac ggaaacgaca
    16441 gcttacgcgc caagtagccc gtattctgcg tctaaagctt ccagcgatca tttggtacgt
    16501 gcctggaagc ggacttatgg tttaccaacc attgtgacta attgctcgaa caattatggt
    16561 ccttatcact tcccggaaaa acttattcca ttggttattc tgaatgctct ggaaggtaag
    16621 gcattaccta tttatggtaa aggggatcaa attcgcgact ggttgtatgt agaggatcat
    16681 gctcgtgcgt tatataccgt cgtaaccgaa ggtaaagcgg gtgaaactta taacattggt
    16741 ggacataacg aaaagaaaaa catcgacgta gtgctcacta tttgtgattt gttggatgag
    16801 attgtaccga aagagaaatc ttatcgtgag caaattactt atgttgccga tcgcccgggg
    16861 catgaccgcc gttacgccat tgatgccgat aaaattagcc gcgaattggg ctggaaaccg
    16921 caagaaacgt ttgagagcgg gattcgtaaa acggtggaat ggtatctggc taatacaaat
    16981 tgggttgaga atgtgaaaag cggtacttat cagtcgtgga ttgaacaaaa ctatgagggc
    17041 cgtcagtaat gaatatcctg cttttcggca aaacagggca ggtgggttgg gaactgcagc
    17101 gtgctctggc tcctctgggt aacttgattg cgctcgatgt tcactccact gattattgtg
    17161 gagatttcag caaccccgaa ggtgtggctg aaaccgtcaa aaaaattcgc cccgatgtta
    17221 ttgttaatgc tgctgctcac actgcagtag acaaagcgga atcagaaccg aatttcgcgc
    17281 aattacttaa cgcgacatgc gtcgaagcga ttgcaaaagc agctaatgaa gtcggagcct
    17341 gggttataca ctactccact gattatgttt tcccaggcaa cggtgacacg ccatggctgg
    17401 aaacggatgc aacagcaccg ctaaatgtct acggtgaaac caagctagct ggggaaaaag
    17461 cgttacaaga acattgcgca aagcatctta ttttccgtac cagctgggta tacgctggta
    17521 aaggaaataa ctttgccaaa acgatgttgc gcctggcaaa agaacgcgaa gagttggctg
    17581 taataaacga tcaattcggc gcaccaacgg gggctgaatt actggctgat tgcaccgctc
    17641 atgccattcg cgtggccgca aataaaccag aagttgctgg tttgtaccat ctggttgctg
    17701 gtggtacaac aacctggcac gattatgccg ctctggtatt tgaagaagcg cgcagagcgg
    17761 ggatcaacct tgctcttaac aaacttaatg ccgtgccgac aacggcctat cccacaccag
    17821 cacgtcgtcc ccataattct cgcctcaata ccgaaaaatt tcagcagaac tttgcgcttg
    17881 tcttgcctga ctggcaggtg ggcgtgaaac gtatgctcaa cgaattattt acgaccacgg
    17941 caatttaaca aattattgca tctcgctcat gatgctagag cgagatgaat taaaaggaat
    18001 ggtgaaatga aaacgcgtaa aggtattatt ctggctggtg gttccggcac tcgtctttat
    18061 cctgtgacaa tggctgtgag caaacaactg ctaccgattt atgataaacc gatgatttat
    18121 tatccgcttt caacgcttat gttggcgggt attcgagata ttctgattat cagtacgccg
    18181 caggatacac cacgttttca acaattgctt ggtgatggaa gccagtgggg gcttaatctg
    18241 caatataaag tacaaccgag tccggatggt ctggcgcaag catttatcat tggtgaagaa
    18301 tttataggtg gtgatgattg tgcgctggta cttggtgata atatcttcta tggtcacgac
    18361 ctgccgaaat taatggatac cgctgttaac agagagagtg gcgcaacggt ttttgcctat
    18421 cacgtaaatg atcctgagcg ctacggtgtg gtggaatttg atgataacgg tacggcaatt
    18481 agtctggaag aaaaaccgca ggaaccaaaa agtaactatg cggttactgg gctttatttc
    18541 tatgacaatg acgttgtaga aatggcgaaa aaccttaaac catctgcacg tggcgaactg
    18601 gaaattaccg atattaaccg tatttatatg gaccaaggac gtttgtctgt cgctatgatg
    18661 gggcgtggtt atgcatggct ggacacgggg acacatcaaa gtcttattga agcaagcaac
    18721 ttcattgcaa caattgaaga gcgtcagggg ctgaaagttt cctgcccgga agaaattgct
    18781 caccgtaaag gctttattga tgctgagcaa gtgaaggtat tagccgagcc gctgaagaaa
    18841 aatgcttatg gtcag
//
LOCUS       DAZSIO010000072        18447 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZSIO010000072 DAZSIO010000000
VERSION     DAZSIO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.072"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..230
                     /locus_tag="ACHX5T_002276"
     CDS             <1..230
                     /locus_tag="ACHX5T_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFR8467458.1"
                     /translation="DGILSQRGGLEIQSQFLRGNEQVYQCQVAFVLPGERVMMAFTYA
                     RTTPLTPADMTRWAEIKKNLRFRMRQEVRTN"
     gene            249..3635
                     /locus_tag="ACHX5T_002277"
     CDS             249..3635
                     /locus_tag="ACHX5T_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR8467459.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDAPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            3638..4018
                     /locus_tag="ACHX5T_002278"
     CDS             3638..4018
                     /locus_tag="ACHX5T_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8467460.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPVYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(4073..4360)
                     /locus_tag="ACHX5T_002279"
     CDS             complement(4073..4360)
                     /locus_tag="ACHX5T_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
                     /protein_id="HFR8467461.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            4489..4998
                     /locus_tag="ACHX5T_002280"
     CDS             4489..4998
                     /locus_tag="ACHX5T_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR8467462.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            4995..6413
                     /gene="phrB"
                     /locus_tag="ACHX5T_002281"
     CDS             4995..6413
                     /gene="phrB"
                     /locus_tag="ACHX5T_002281"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFR8467463.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(6563..8044)
                     /gene="dtpD"
                     /locus_tag="ACHX5T_002282"
     CDS             complement(6563..8044)
                     /gene="dtpD"
                     /locus_tag="ACHX5T_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR8467464.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            8315..9058
                     /gene="ybgI"
                     /locus_tag="ACHX5T_002283"
     CDS             8315..9058
                     /gene="ybgI"
                     /locus_tag="ACHX5T_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR8467465.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            9081..9737
                     /gene="pxpB"
                     /locus_tag="ACHX5T_002284"
     CDS             9081..9737
                     /gene="pxpB"
                     /locus_tag="ACHX5T_002284"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR8467466.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            9731..10663
                     /gene="pxpC"
                     /locus_tag="ACHX5T_002285"
                     /pseudo
     CDS             9731..10663
                     /gene="pxpC"
                     /locus_tag="ACHX5T_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            10653..11387
                     /gene="pxpA"
                     /locus_tag="ACHX5T_002286"
     CDS             10653..11387
                     /gene="pxpA"
                     /locus_tag="ACHX5T_002286"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR8467467.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            11423..12214
                     /gene="nei"
                     /locus_tag="ACHX5T_002287"
     CDS             11423..12214
                     /gene="nei"
                     /locus_tag="ACHX5T_002287"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR8467468.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(12211..13257)
                     /locus_tag="ACHX5T_002288"
     CDS             complement(12211..13257)
                     /locus_tag="ACHX5T_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR8467469.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(13409..14485)
                     /locus_tag="ACHX5T_002289"
                     /pseudo
     CDS             complement(13409..14485)
                     /locus_tag="ACHX5T_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(14482..15213)
                     /locus_tag="ACHX5T_002290"
     CDS             complement(14482..15213)
                     /locus_tag="ACHX5T_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR8467470.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            complement(15228..17687)
                     /locus_tag="ACHX5T_002291"
     CDS             complement(15228..17687)
                     /locus_tag="ACHX5T_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR8467471.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKIPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DGNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            complement(17738..18304)
                     /locus_tag="ACHX5T_002292"
     CDS             complement(17738..18304)
                     /locus_tag="ACHX5T_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8467472.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
BASE COUNT         4548 a         4439 c         5107 g         4353 t
ORIGIN      
        1 atgacggaat attatctcaa cgaggcggtt tggaaataca gagccagttt ctccggggta
       61 acgagcaggt gtaccagtgt caggtggcat ttgtattgcc gggtgagcgg gtcatgatgg
      121 ccttcaccta cgccagaacc acaccgctga ccccggcaga tatgacgcgc tgggctgaaa
      181 ttaaaaagaa tctgcgcttc aggatgcggc aggaagtcag gacgaattaa tcatacaggg
      241 aacgggacat ggaagaggca ttctgggcgg caagacaggg tgatgccctg ctgcatacgt
      301 cgtttatggc agatgtgctg ggggcggtgc tggaggtggc cgcaaatgtc gttattgatg
      361 ccctcattgt cggcacaacc tcgttactgg cgacgtcagt ggggatcacg atggggtgca
      421 cggccgtcgt gctgaccggt tttgtggcgg gtgtggcgat ggtgtacacc ggcgtgacag
      481 aaaaggtcag cgaagcctgt tcagccctcg caaatatgct tttcccgcct cagattgaag
      541 gttacatcct caccggctct ggtgacacat ggatcaacag caaacctgcc gcgcgggcag
      601 cggcgactgc ggcatcgcgg caggacatag aggcgcagga agcgcaggcg aaggcagaac
      661 aggaagaagc gcagcgtcag gaggatgcca gaacgttccg ggatgtggcc ggggaatatc
      721 tggaaatggc gggggccata gccctggcgg ttaacccggt gacagggcct gtgctgatgt
      781 cccgggctct gcacgaccag ctcagtacgg aagaagggcg ttctgaaatg gtggatggcg
      841 tgacacattt tgtgtcggaa ctatggcagc ccactgtggc cagtgcggcc ccgggatcca
      901 ccccgacccc tgacgataaa atcgactgcc ataaacatcc ttcctcactg acgcagtttc
      961 tcgcacaaaa aaaagccgcc tttctggatg cccccctggg aacggccctt aacctgctga
     1021 atccgggcgg tatgctggaa accgcctttc agggagtgaa tgcggttatt ggcagcatca
     1081 gtaatctgtt caaaggcgat gacgagccgc ccgcagcgga atatatcgcg gaaggcgcgc
     1141 gggatgtgcg tatcaacagc cagccggcga cacgcagcgg ggcacgctgt acctgcgagg
     1201 ccagggtggt gaatgagccg gggaacgggg cgtttgtgtc accggatgtg cgcattggcg
     1261 gtccgttact ggtggtcagg gatatcagaa gcggcaggtc gcagataacc ctggtggcaa
     1321 ccgtggcgct gatgtttctt cgcccgggca aaatgctttc taaaatcgcc tgttttgctg
     1381 ccagtgtcgg gatgagcatg ctgacgcaga aggccaccag tgcccttccg catccggtca
     1441 atgccgccac cggggccaaa tacctggccg atgacgatga cttcgatttc agtctgccgg
     1501 gccactttcc gctggactgg cagcgcgtgt acagcagtcg tgacgaacgt acggaaggaa
     1561 tgtttggtca gggctggagt gtaatgtatg aagtcagcct cgtgtgcaca ccgggtagcg
     1621 cagacgaaaa ctgcatgacc tttgtgtcgg gcatggggcg acgcttggat atggaagccg
     1681 tactgccggg gggcgggttt tacagccccg gtgaagggct ggcggtaagg cgtggtgagc
     1741 aggggcactg gcttatcagc agcgatgacg ggcagtgttt tctgtttgag gctgacccgc
     1801 accacccgca gcgccagcgg ctgaaaatgc tgggcgaccg taacagcaac tgtctgaatc
     1861 tgtactacga cgaccggggc cgcattgtgg aaatcagcgg cgaacagcag cgtccgtgca
     1921 tccgtctgta ttacgaactg gcggcgcatc cccggcgggt gacacagatt tatcagcatt
     1981 tccctgagac tgcaccttta ctgctgcgtc gttacagcta tgacgaggcc ggccacctga
     2041 acggcgtgta cgacagcacg gggcacctgc tgcgtgagtt tgcttacgac gaaaaccact
     2101 gcatgacgct gcaccgccag ccgggcggcg aagggtatta ttaccagtgg ggctggtatg
     2161 aaggtccgga tgacgcggca tggcgggtga cggggcacca cacggacagc ggtgaacagt
     2221 accggctggc gtgggacctg gcgcagcgca ggctgtgcgt gacggacgga ctggggcgca
     2281 cacgttatca ccagtgggat gcgcagaatc aggtgacggc gtaccaggac gaagccggac
     2341 aggtgacgac attccgctgg agcgacgaag aacgactgtt gctggggatg accgaccctc
     2401 agggcggcaa atggcgctat gtgtatgacc tgcaggggca tatcacggag acgcacgacc
     2461 cgctggggcg ggtggagcag gcgcagtggc acccggtgtg gcaccagccg gaaacggagg
     2521 tggacgcggc gggaaacctg ctggggaggc gcgcggggga aagggccacg gcagaaaaca
     2581 gcgtggtgcc gttcaaccgg ctgatgtctt accgtggtgt gcactaccgt tatgacgaat
     2641 ttggccgtgc ggtggaaaag gagggccgga gcggaacgca gagttaccgt tatgatgcgg
     2701 aacaccggat ggtggaagtg acgacagcgc gggggacata ccggtatgtg tacgatgcac
     2761 tggggaggcg gacggaaaaa cagcacatca gccctgacgg taagccgtac aaccggacaa
     2821 aatttttatg ggacggaatg cggctggcgc aggagagcag gccggaggga acaggcagcc
     2881 tgtacatcta cagggaccag gggagttatg aaccgctggc gcgggtggat aaggcgggca
     2941 aagagggccc gaaccggata ctgtattttc acacggatgt gaacggcgcg ccggaagaga
     3001 tgacggacag cgacgggaaa atcgtgtggg agacggggta tcaggtctgg ggtaacacga
     3061 tacaggagaa ggaccacggc ggggtggagc agaacctgcg ctaccaggga cagtacctcg
     3121 acagggaaac ggggctgcac tacaatctgc acaggtacta tgatccggat gtcgggcggt
     3181 tcatggtaac tgacccgata gggttgagag gcggtttaaa tctctacagt tatgcaccta
     3241 atcctctaaa atatgctgat ccacttggat taactccatg tgctgttagt aaccaaaaag
     3301 cgaatcgttt attagatagt agtgaaacca aagttactgt taggtctaga agtgacgctg
     3361 aacaattgtt tatggataga tatttaggtc ataattataa aaatatgaca ggtgagtcag
     3421 gccccagcac taagaactta atggaatatc ttactgaaaa taaaacaaaa gcaggttcat
     3481 atcattggga tgatataaaa gatccttctg taacaaaacc ttcatataga gtgtcaggtc
     3541 atgggccagg aaatcctgat ggtgatttac cacatttaca agttcatcaa catggtggaa
     3601 gtgttagaca tatatttttc ccgtgggaga cataataatg aacaatctca atacattatt
     3661 aagtggaaag gttttgctca aagaaattag tccattaatg cctgtatata gaacatggat
     3721 tgaaatttca ttgatcgatg aattaaaacc atcttatcct tttagattag atgaatatgc
     3781 tatgataggg cattcacctt atgccaatga atgtaataaa gatgaagcta aatttaaact
     3841 acgaatatca tcatttttag cttctgacat agataatgaa tacgatccat catatgatta
     3901 tgttggaaaa tatgaaatta tcgcttcact taatgagtta aaaactaggt taagtactct
     3961 tcatgtaaac ttggagcaat ttataaattc atcggaagat gatgagtatc cattataata
     4021 agacagaata aaaagctagg aaggttttaa atcttcccag cttttttaat atttaacttt
     4081 cgttcagcac actaaacatc ccatcccgca gttcctgcac ctcgtcactg tcagcgatga
     4141 tggtcaggcc cccctccgaa atcttcaccg tgacaccccg tccggtatca aatccggcct
     4201 ctttcagcca gtcgccctta aggtgcagac tgggatgttg tgaataatac cgggctatac
     4261 cggttttgcg gtcctggtgg cgggctctga catagccgac tttatagcgg cgctgtgttt
     4321 tggaaatagt gacttctggc gtaatatcgt gttcagccat gattaactac cttgattagt
     4381 tgcttgcggg gagcgcgctt tcgtaatctt gccctgctag cggattaact tcacaaacat
     4441 catttaccaa cgcctacact tactcctgta taccgttcag gagcagtaat gaatctacaa
     4501 cgatttgatg acagcaccct aatccgtatc ttcgcccttc atgagttaca tcgactgaaa
     4561 gaacatggct taacgcgcgg ggcgcttctc gattatcaca gccgctataa actcgtcttt
     4621 ctggcccatt ctcagcccga gtaccgcaaa cttggcccgt ttgtggctga tattcatcag
     4681 tggcaaaatc tggatgattt ttacaaccag taccgccaac gcgtaattgt tttgctttct
     4741 catcccgcca acccgcgcga tcacaccaac gttttgatgc acgttcaggg ttattttcgc
     4801 ccgcatattg attccacaga acgccagcag ctggctgcgc ttatcgacag ttatcgccgt
     4861 ggcgagcaac cacttcttgc gccgctgatg cgtatcaaac actatatggc gctttatcct
     4921 gacgcctggc tttcagggca gcgttatttc gaactttggc cgcgtgtgat taacttgcgc
     4981 cattcaggag ttttatgact acccatctgg cctggtttcg ccaggattta cgtctgcacg
     5041 ataatctcgc actggctgcc gcctgccgca attcgtctgc acgcgtgctg gcgctgtata
     5101 tcgctacacc acgcaagtgg gagacgcata acatgtcgcc gcgtcaggct gaacttatca
     5161 atgctcaact gaatgggcta caaatcgcgc tggcggaaaa aggtattcct ttattgttcc
     5221 gtgaagtgga tgactttgtc gccagtgtcg aaatagttaa acaggtgtgc gcggaaaaca
     5281 gcgttactca tcttttttat aactatcagt atgaggtgaa tgagcgagcg cgggatgtgg
     5341 acgttgaaag agcgctgcgt aacgtggtgt gtgaaggatt tgatgacagc gtgatcctgc
     5401 cgccgggggc ggtgatgacc ggcaatcacg agatgtacaa agtctttacg ccttttaaga
     5461 atgcctggct gaaacggctg cgggaaggga tgccggagtg cgtcgctgca ccaaaagttc
     5521 gtagtagcgg atcgataaag cccgcgccat ccattacgct gaattatcct cgtcagtctt
     5581 tcgatactgc gcatttcccg gtggaagaaa aagcggcgat tgcgcaatta cgccagtttt
     5641 gcgagaacgg tgctggagag tatgagcaac aacgagattt tccggcagtg gaaggcacca
     5701 gccgtttgtc cgccagcttg gcaacgggcg ggttatcgcc tcgccagtgt ttgcatcgct
     5761 tgttggcgga acagccgcag gcgctggacg gtggggccgg tagtgtctgg cttagtgagc
     5821 tgatctggcg cgagttctac cgtcatctga tgacgtatta cccctcgttg tgtaaacatc
     5881 gtccatttat tgcctggacg gatcgtgtgc agtggcaaag caatcccgca catttacagg
     5941 cctggcagaa aggcaaaacg ggatacccga ttattgatgc cgctatgcgt cagcttaaca
     6001 gcactggctg gatgcataac cggctacgga tgattacagc cagttttctg gtgaaagatt
     6061 tgttgatcga ctggcgcgaa ggcgagcgat atttcatgtc gcagctgatt gatggtgatt
     6121 tggcagctaa taacggtggc tggcagtggg cggcttctac gggtactgat gctgcgccgt
     6181 attttcgtat tttcaatccg ataactcagg gcgagaaatt tgaccgtgag ggcgagttta
     6241 ttcgtcgatg gttaccggag ctgcgcgatg taccagggaa agcggtgcat gagccgtgga
     6301 agtgggcgca gaaagcaggt gtgaagctgg attatccgca accgatagtc gagcacaaag
     6361 aggcgagagt acaaacgttg gcagcgtatg aggcggcgcg gaaggggaaa taacagacgc
     6421 gtcaggcaat cgagcccaga tgccggatgc ggcgtgaacg cctaatccag cctacaaatt
     6481 tgtgcaaatt caataaattg caggaaatac gtaggcctga taagacgcgt caagcgtcgc
     6541 atcaggcatc ggtgctggcc tattatgact ccagcgccag cgcgcggttt ctgaatttca
     6601 gcgcctgata cagccaaatc atcagtacca gtcctacaca cgccagtgcg ccccaggtaa
     6661 tttgatcaaa tacttcaata tatgcattga tggagtagtt gatcgccccg gaagcatcaa
     6721 acgaagcctg cgatgtctga tcggcaatca cgcccgccag atagttcgcg atcgcgccag
     6781 aaagcagcat gtagatgccg gttaatacgc cggtcacgcc cggaatttca atgcgcgtaa
     6841 tttgcgacat ggcaaccggg tcgataaaca gttccgcaaa gcccatcacc gcgaggccta
     6901 acaccatcag tggcagagaa gagtgaccat acattgcgga ccagcgggcg cttaaggtca
     6961 gaatgcagaa tccggcgctc atcaggccaa ggccaagagc aaattttccc cagatgcgca
     7021 cggtacgatt acctgcgacg ctttctttaa ccacccacgc caggaacaca ccgcacagca
     7081 taactgcgaa ggcattaatc gactggaaca tcgcggtcgg aacggtataa ccaaacatat
     7141 cgcggttaac gaagcggtcg atataaaggc taatcgagct accgccctgt tgtgcgaagg
     7201 cccagaacaa catactgaag aaggtgagcg tcacaatcag ccccagctcc ttccgctgtt
     7261 tctggttttc tgctttgcga taaatttttg ccagtacacc caggccaata atagtcgcga
     7321 caattaaggc gtataccgac cactctttcc agaacagcac ggtaatcagt gcaggcgttg
     7381 ccaccagcag aaccagcagc catccccagt tcggcaggag aaagtttgtc gcacgcagta
     7441 cttttttgtt aacgccgcgg gtatgagtga aatgacgatt gccacataag aaaatgacca
     7501 gacccgcgat cattccaacc gccgccaggc caaagcccat cgcccagctg tactcttcct
     7561 gggcgtaacc acaggcgata ggtgcgataa tagaccccac gttacccgcc gcatacatca
     7621 gcgagaaacc gccatcacga cgcggatcgg ttggctcata cagctcgccg agcagacagc
     7681 ttacgttaga tttaaacagg ccatagccgc agacgataat cgccagggac agatagagga
     7741 atgacggatg gatctcactg gcacccagca ccacatgacc gatcgccatc aacaacgccc
     7801 ccagcatcac cgccatgcga ttgccgagaa ctttatccgc caaaaagcca ccgaggattg
     7861 gcgtgacata caccagcgaa cagtaggcgc taaataactc gtaggcgtga gtatcgttgt
     7921 attttagttg attggtgaga tagagaatca gcagggcacg catgccgtaa aagctaaaat
     7981 attcccagat ttgcagcgca acgacatagt aaatagcgcg cggttgtgat gcgtgtttat
     8041 tcataataat tccacaattg acggcaacgt cacgtaatag ttgcgcggcg tgcttgtgtt
     8101 tccttaaagt taaaactttg atacagatct gattatttat gcaatatgct gtctgattgc
     8161 ataaatatac attagctgaa gcgtgatgat ataaacaatt tgcgtctttt ggcgtcgggt
     8221 ttgtttctta atatgtcaaa agattggcta tcgacgaaca ggcgatgtaa cgctatgtta
     8281 acggtgtagc tatcagaata cggaaggcgc gaagatgaaa aacaccgaac tggaacaact
     8341 gattaacgaa aaactgaaca gcgcggcgat tagtgattac gcgccgaatg gtttgcaggt
     8401 ggaaggcaaa gagacggtgc aaaaaattgt taccggtgtc accgccagcc aggctttgct
     8461 cgatgaggca gtgcgtctgg gcgcggatgc ggtcattgtg catcatggct acttctggaa
     8521 aggagagtct ccggtcattc gcggaatgaa gcgtaaccgt ttaaaaacgt tgctggcgaa
     8581 tgatatcaac ctgtatggct ggcatttgcc gcttgacgca catcctgagc tgggcaataa
     8641 cgcacagctg gcggcgttac tggggatcac ggtcatgggc gaaattgagc cgttggtgcc
     8701 gtggggagag ctgaccatgc ctgtgccggg aatggaacta gcctcctgga ttgaagcgcg
     8761 tctgggacgt aagccgttat ggtgtggcga taccggacct gaggtggtcc agcgcgtcgc
     8821 ctggtgcacg ggcggcgggc aaagttttat cgatagcgcc gcgcgttttg gcgtggatgc
     8881 ttttattact ggcgaagttt ctgaacagac cattcattca gcccgcgagc agggattgca
     8941 tttttatgct gcaggtcacc atgccactga acgcggtggt attcgcgcat tgagcgagtg
     9001 gctgaatgaa aataccgatc ttgatgtgac ctttattgat attcctaatc ctgcataacg
     9061 aataatcaga gggatcgaaa gtgcaacgag cgcgttgtta tctgataggt gaaacggcgg
     9121 tagtgctgga actggaaccg ccggtgacgc tggctagcca gaaacggatc tggcgactgg
     9181 cgcagcgtct ggtggatatg ccgaatgtgg ttgaagccat tcccggcatg aacaatatca
     9241 cggtgatttt gcgtaatcct gagtcgctgg cgctggatgc catagagcgt ttgcaacgct
     9301 ggtgggagga gagcgaggcc ctggaacctg agtctcgctt tattgaaatt ccggtggttt
     9361 acggtggtgc aggcggaccg gatttggcgg tggtcgcggc gtattgcggg ttgagcgaaa
     9421 aacaggttgt tgaattgcac tcctccgtgg aatacgtggt ctggttttta ggttttcaac
     9481 cgggcttccc gtatctcggg agtttgccgg aacaactaca cacgccacgg cgcgctgaac
     9541 cgcgcttgct cgttccggca ggttctgtcg ggatcggcgg gccgcagact ggtgtttatc
     9601 cgctggcaac gccgggtggc tggcagttga ttggtcatac ctcactcagc ctgtttgatc
     9661 cggcgcgtga cgaacccatc ttattacgtc cgggagacag cgtgcgattt gtaccgcaga
     9721 aggagggaat atgctgaaga ttattcgtgc gggcatgtac accactgtgc aggatggcgg
     9781 tcgtcacggt ttttgccagt cgggtatcag ccactgtggc gcactggata tgcctgcgct
     9841 gcgcattgct aacctgctgg tgggtaatga cgccaatgcc cccgcgctgg agatcacgct
     9901 cggtcagtta acagttgagt tcgaaactga tgggtggttt gccctgacgg gtgcaggttg
     9961 cgaagcgcgg ttggatgata acgccgtctg gaccggctgg cgattgccga tgaaagcagg
    10021 ccagcgttta acgcttaaac gcccgcagca cgggatgcgc agttatctgg cggtcgcggg
    10081 tggtattgat gttccgccgg taatggggtc atgcagcacc gatctcaaag tggggattgg
    10141 cgggctggaa ggccgtttac tgaaggatgg tgactgactc ccgattggca aatcgaagcg
    10201 tgattttatg gaagcgcagg gcgttaaaca gctgctgtgg ggcaaccgca ttcgcgcctt
    10261 gccggggccg gaatatcatg agttcgatcg cgcctcgcag gatgcattct ggcgttcgcc
    10321 ctggcagctt agctcgcaaa gtaaccgaat gggctatcgt ttacaggggc aaattttaaa
    10381 acgcatcacc gatcgcgaac tgttatctca cggtttgtta ccgggcgtgg tgcaggtgcc
    10441 gcataacggg cagccgattg tgttgatgaa cgacgcacag accaccggtg gttatccacg
    10501 tattgcctgt atcattgagg ctgatatgta ccatctggcg caaattccgc tcggtcagcc
    10561 gattcatttt gtccagtgtt cactggaaga ggcactgaaa gcgcggcaag atcagcaacg
    10621 ttattttgaa caattagcgt ggcggctgca caatgaaaat tgacctgaac gccgatctgg
    10681 gcgaaggctg cgccagcgac gcagagctat taacgctggt ttcctctgcc aatattgctt
    10741 gtggatttca tgcaggcgat gcgcaaacca tgcaggcttg cgtgcgtgaa gcaataaaaa
    10801 atggtgtcgc gattggcgct cacccgagtt ttcccgacag ggaaaatttt ggtcgcagcg
    10861 ccatgcagct gccgccggaa accgtttacg cccagacgct gtatcaaatt ggcgcgctgg
    10921 caacgattgc ccgtgcgcaa ggcggcgtaa tgcgtcatgt caaaccgcac ggcatgttgt
    10981 acaaccaggc ggcgaaagaa gcacaactgg cagacgccat cgccagagcg gtatacgctt
    11041 gcgatccggc attggttctc gttgggctgg caggaagcga gctgattcgt gcaggcaagc
    11101 aatatggcct gacaacgcgc gaggaagtgt ttgccgatcg cggttatcag gctgacggct
    11161 cgctggtgcc gcgaagtcag ccaggcgcgt tgattgaaaa cgaagaacag gcgttggcgc
    11221 aaacgctgga gatggtgcaa cacggcagag tcaaaagtat caccggcgaa tgggcaacgg
    11281 tcgcggcgca aacggtctgc ctgcatggcg acggcgagca cgctctggcg ttcgcccgcc
    11341 gattacgagc tacatttgcc aaaaagggga ttgttgtcgc agcataaccc cgcttaataa
    11401 agaatgaaaa aaggatatca ccatgcctga aggcccggag atccgccgtg cagcggataa
    11461 cctggaggcg gcgatcaaag gcaaaccact aactgatgtc tggtttgcct tcccgcagtt
    11521 aaaaacttat caatcacaac ttatcggtca acacgttacc catgtggaaa cgcgtggtaa
    11581 ggcgttgtta actcattttc ccaacggttt aacgctctac agccataatc agctttacgg
    11641 cgtctggcgc gtggttgata ccggcgaaga gccgcagacc acgcgagtat tgcgggtaaa
    11701 actgcaaacg gctgacaaaa ccattctgct ttatagcgcc tcggatattg agatgttgag
    11761 gccggaacaa ctgaccacgc atccgttttt acaacgcgtt ggtcccgatg tgctggatcc
    11821 gaatctgacg ccggaggtgg taaaggaacg gttattgtcg ccgcgctttc gtaaccgtca
    11881 gtttgcagga ttactgctcg atcaggcgtt tctggctggg cttggcaatt atttgcgggt
    11941 ggagatcctc tggcaggttg ggttgactgg aaatcataaa gcgaaagatc tcaatgcggc
    12001 gcaactggat gcactcgcac acgcgttact ggagattcct cggttttctt acgctacgcg
    12061 ggggcaggtg gatgagaata agcatcatgg ggcgctgttt cgctttaagg tttttcatcg
    12121 tgatggcgaa ctgtgcgaac gctgtggcgg catcattgag aaaaccacgt tgtcctcgcg
    12181 cccgttttac tggtgtccag gctgccagca ctaggccgac cgcttcggcg cataggttga
    12241 aataaaccgc gcaatggctg gccccgtcag caaaatactg aacaggcgca gggtttgcat
    12301 cgccataata agcgccatat cggcattgct ccctgcggcg atgaccgcca cggtatcaag
    12361 cccgccggga ctggtggcga ggtaggcagt cataaaatca atatgcataa accgggtcag
    12421 tccccacgcc atacctgcac aaatagccag cagagcaaaa atcgacagca ggatttgcgg
    12481 tagcgggcgt agtgcccgca gtaagatttg tttatcgaaa ccaagaccaa tccgccagcc
    12541 aattgccata tacgccatcg ccagcagcca ttccggtagt tcgatggtga tgagctgacc
    12601 agactggagc actgcgcccg ccagcatcgg gatgagcatc gtgccggaag gaagacgcag
    12661 catgcatccc gccgtaccgg caacgactgc gagcaaaatc gtaagcagga gattaatgct
    12721 cactggcggg aaccagacga tttgctggtt aaccgcttca gcgttatcac ccagcatcat
    12781 gcgggtgacc agaacagcgg cccccgcgac gaacagcact cgcagatatt gcataaacgc
    12841 caccaggcga atatctgcgc cgtaatcttg cgccatagcg accattgccg ctgcgccgcc
    12901 tggagaagaa ccccaggcac cggtatttcc aggcagtgag ctatagcgca ccaataacca
    12961 acccacgatg gcgctggaaa gcagcgtcac cagtaaaata gcaagcacga tcggccagtt
    13021 ggcggccagc gtggtgagaa tcgaaccagt gaggttttgc gcaatcatgc agccaagaat
    13081 ggcctgcgcg gcgagaaaag cggagcgggg aagttgcaga gtaattccgc gcatactgaa
    13141 gatgatcccg gcgatcatcg gcccaagtaa tagcgccgcc gggagatgta ccgcgaggaa
    13201 accaatagaa aggagaagtg atagcacaca taacattccc cactgcaaaa ctggcatcct
    13261 tcgctccctc attattttct ctttggtgta tcgatacagc ataaagaaag ccgggctagg
    13321 aaaaaataac agcaatcaag gaaaagggga aaatcagcaa ttttctgaaa gagatgccct
    13381 ttccggctgc aaagggcatc atggcaaatc agttatagtt aattttaaaa agcaccactg
    13441 tatcgaaggg cccagcctcg atgggggtac ctgcaatgga cgaaagttca gctgtatagg
    13501 ttttttcgta gaccaacatg tcgctggtga aatcggcgta ttcagcatat ttattgaagg
    13561 tataaggcga ggcgttttca tccagcaaac gtagcttcag accattacca atcaacaatg
    13621 ctttatcttc ttcaaccaac gtttcttcgg tataaaagga ggaactgact ttaaatccgt
    13681 cggtgcattg gtcattttgt gattttgtcg tcttaatact gaatgttttc gacattgtgt
    13741 ggcgacgaat atccagcaaa ttaaatttac caaaatcaat catctgtgtt tccggtgaaa
    13801 tggaaaaatt gacgctgcaa tccagcacgc gaatgttttc cagaccagta atatgatatt
    13861 tcaggttttg ggcagtaggg tcttcgttaa cgctaccggc accgtcaaat tgcacgacga
    13921 tatagtcgct aagcgtactt tgataatcat gcggcggcat ttctcgtatt ttgacgtata
    13981 accgcatgcg agcgagaaag gttcgcgaca tgtgaatatt agcgggatca cccgagcaaa
    14041 ttttgttctg ccatcccatc aggggtataa atgtcaatgt tcttactgtc gatacactgg
    14101 ttagtgtcga tgcggctttt tccctggttg gcgtcataat caacgccatt gtaagttact
    14161 ccgagttgat aataacgatc ctgcaccccc gggtaggggt ttacccatac gtagacatgt
    14221 tcgctttcaa agttgccgtt ggtattgttg tcacaataga cgggaatttt aatatcgtca
    14281 gattcccaga tcttatttta atatcgtcag attcccagat cttatcgccg agtttggcat
    14341 tgcctggtac agcaaaaggt tgaatcgctt ctgatttttc taccgagcca ccggatgaac
    14401 caaagtagca gttcagggca agcgcagact tcaacgacag aaataacaga cagatgacga
    14461 gcaacactcc cttgccagca ctcatttctt cgcctccacg gcttgtaaat ggcattgtcc
    14521 gctgcagttg agcgtcgtca tgcgtaacgc gccgtaatca tccatataac cgagataaaa
    14581 ctggctgccg ctataatttc cggtgtttgt gttgaccgta ctaaaaggcg caaccatcac
    14641 ggttttgaag ccggggagaa cgcctttttc atttcgtccc aggtaggcaa tggtcagata
    14701 atacgcggta ggatttttca gtgtcagttg gttgccctgc tggctgacct gtaactgcag
    14761 ttcgaccttt tctcctgctt tcttgcgtaa tgcagccggt cgccagaaca ccttgatacg
    14821 actctgaatg gcgacttgca gaacggcatg gtcgctgttt ttttccgggg cgggaggaat
    14881 ttcacgcata ttgtagaaaa atagcgtttc gcgatcgccc ggtaattttg tggtcgaggc
    14941 ttgctttact atccgtacct gtgttgttgc tttcggttcc agacgttgta tcggcggcag
    15001 ggcgaccaga agatcatcgc ttttctcccc tttttcattt tcgagccagg aataagcgag
    15061 gtaggggagt ttatcgctac gattatccac ccgcagactg gtagctttat cattagcgtt
    15121 aaacacaata cgagtgcgat cgggttgtag tgcagcgtgg ctgcacagac tggcaaccaa
    15181 caacaaaagg ggtagtcctt tcataaatgt cattttcatt tccttaatta atgacaaggt
    15241 aaaatcaggc gatttgcggt gtcttccata tgttcaggaa gatggagcga gcaatgctgg
    15301 ctatcacccc agaccacggt aaacttttga ttttcagcga ctccgcttaa ccaggcatgt
    15361 ccttcctcgc caaccatccc cacgctaatg ccgctgtcat cctggcggat atccgctccg
    15421 aggggaggaa actgaccgct ggcgttgcga atgatgacgt taccgtcttt accggaacgg
    15481 gaagccagtg atttgtaacc tatcgcgcct tcaatccacg tttctttgat aacgttttct
    15541 gcaactgtta cgccgtcggg taagtcattc atgttcaccg ccacggtgga aggctggtaa
    15601 ctggaaatca acggcaccac cgcaatgcca aaatggttgg tgtagtcgag attgccctga
    15661 accggaatat ctgccacgcc atcggtgctg accatcaggc gtggttcatt ggtggagctg
    15721 cggcgatgaa acgctgcacc atattgggtt gcggtgaaag aaccgctcca gctgctgctg
    15781 acggaactgt aatcattggc ggcataggta ccagaaatat cccactcacc cgctgaactc
    15841 aggtgctgat agttaccgct cacctgggct ccattgtcag gacggtcgga ttgcagtccg
    15901 gcagacatgc cccagctatt acgttcatcc agcgtatcgt tccacgacat gcggtgtgtg
    15961 gtgctgtggc tactgttttg catgtcatag ccaacccgac caccgttacc gaaggggagc
    16021 gaaatcgaca ggtaaatctg gttgtcgcga tctttatctt cgtaatgggt tgtattgaat
    16081 gacgtagaaa tagagatatc tctccagtca ccaatatcaa cattaaaacc tgccgtgatg
    16141 ttggcggtcg tggaggcgtc tgcattccac caggtttgat gtagcaggtt ggcgtaaaga
    16201 ttgaggttta gtggggtaat cggttggccc acagataagc tgatcgtctg tttttcgtcc
    16261 tgcgcatcgc tgtcgttgta tttgtgatcc agatagttgg cgtagctgtg aaaatgacga
    16321 tcggagaaac gataagcggc gagggaaatc gtgctgttag tggcatccac ttgtttgctg
    16381 taattaaaac ggtagcttaa gccccgctcg tcctgctgag tatcaaaatg gctactggcc
    16441 cacgtgacat caaacgacag cgcaccaagc cacagcatat tttgcccaat acccattgcg
    16501 gcagaatggt agtcatcacc agaaagcagc aggccgctgt acagcgaggt gtttgacagc
    16561 atcccccagg aaacttcatt gctaaaaaag gtttcatttt cagtttgatg tgacatggag
    16621 ggccgtggct gacccgcggc cagtttatag cgaacctgtc cctgacgagt caggaagggc
    16681 gtcgatgctg ctgaaacctg gaaattgttc acccgaccat cttcttccgt cactttgaca
    16741 tccagtgtgc cctgaacaga ctgattaagg tcgtcaatga taaatgggcc tggtgggatt
    16801 tttttctggt aaatgacgcg gcctgattga ctgatcgtca ccgtggcatt ggtctgtgca
    16861 ataccgctaa tttgtggggc gtagccgcgt agttcccatg gcaacattcg atcgtcactt
    16921 gccagtgccg cgccggtata agaaaaaccg tcgaaaatat tgggactaaa atccgtttcg
    16981 ccgagggtta attttgaacc taattgcggt aacgggcgaa aaagataggt acgtgatatt
    17041 tcgcctgact gttcatggtt atcatcgcta tcagtatgat tcaactgata atcactgcgt
    17101 aagcgccatg cccccgtgtt gattccggct gttccatagg cgttaagatt agtgctgctg
    17161 ctgccatcct gtgggcggta gctgctggca aacaagttgt aatccatcag gacaccggcg
    17221 acaccttctt tccatgtgga gggtggggtc cagttttctg agtgccacgc cagccaggct
    17281 tgcggaatac taatattcag ttgctgattg gcttgatcga aattgaagag catttcaggt
    17341 cgggagctaa aatcgacgca ctgatttatc aatggtaacg actgacggac ttcaggtttt
    17401 aagccaaatt tatcgaccag taaatcattg atgcacggaa tggttttatc gtcatttttg
    17461 tgccagttaa tcttttgctc gttactgatt tggttattat ttaccgcaac gctaacaaaa
    17521 tactcaccgg gagcaatgac tcctttttcc tttaataatg aaatatcaat gcggtcgcgc
    17581 attgatttat cgagaacatt cagattgaat tcgaccgcca atgcgcaagg catcgccacg
    17641 accaggcagg atacaaaaga gagtcgataa atattcacgg tgtccatacc tgataaatat
    17701 tttatgaaag gcggcgatag cgccgccaaa gtaatactta tttataatcc agcacgtagg
    17761 ttgcattcgc cgtaactgcg cctggcgtta caggtgtagc ctgatcaatt tgttccatcc
    17821 aggcaaagaa attcagcgtt tgttcgctgg aggtcggagc caggtcgata tctggagtag
    17881 cggtgcctaa aacgatattg ctttgatctt tattcattaa acgtacgccg acgccggtgg
    17941 cttcacccgt gctggtgttg ttgagaatcg gatctgcgcc ggtggttttc gctgagctat
    18001 caaaggtgac tgcaacttta gaaatcttgc caccggaacc gttgctggag ttttccagat
    18061 cacagttgac taagagcagg tcgacagcaa cggcatcgct gtgatgtgac tgattaatgt
    18121 ggctggtggt cacctgaccg agttcaacct ctttatcaat atcatcctga tgtatttcac
    18181 acggtgcttc aataacttca cctttaaagt gaatttcacc ggatccttca tcagccgcat
    18241 aaacaggtgc tgtgaacagc agagatacgg ccagtgcggc caatgttttt tgtcctttaa
    18301 acataactga gtcctttaac agaaaagaaa aaagtatatc ggtgcaagaa tatcttattt
    18361 gattgtcgaa tgtttttagt ttttcttaaa aatattatta attttattaa atgtaaaagt
    18421 aattaattga tggtatgaat taaatgt
//
LOCUS       DAZSIO010000073        18251 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZSIO010000073 DAZSIO010000000
VERSION     DAZSIO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18251
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.073"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            15..654
                     /locus_tag="ACHX5T_002293"
                     /pseudo
     CDS             15..654
                     /locus_tag="ACHX5T_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            757..1080
                     /locus_tag="ACHX5T_002294"
     CDS             757..1080
                     /locus_tag="ACHX5T_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HFR8467473.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1147..2604)
                     /gene="nuoN"
                     /locus_tag="ACHX5T_002295"
     CDS             complement(1147..2604)
                     /gene="nuoN"
                     /locus_tag="ACHX5T_002295"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR8467474.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2611..4140)
                     /gene="nuoM"
                     /locus_tag="ACHX5T_002296"
     CDS             complement(2611..4140)
                     /gene="nuoM"
                     /locus_tag="ACHX5T_002296"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR8467475.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4304..6145)
                     /gene="nuoL"
                     /locus_tag="ACHX5T_002297"
     CDS             complement(4304..6145)
                     /gene="nuoL"
                     /locus_tag="ACHX5T_002297"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR8467476.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6142..6444)
                     /gene="nuoK"
                     /locus_tag="ACHX5T_002298"
     CDS             complement(6142..6444)
                     /gene="nuoK"
                     /locus_tag="ACHX5T_002298"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR8467477.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6441..6995)
                     /gene="nuoJ"
                     /locus_tag="ACHX5T_002299"
     CDS             complement(6441..6995)
                     /gene="nuoJ"
                     /locus_tag="ACHX5T_002299"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR8467478.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7007..7549)
                     /gene="nuoI"
                     /locus_tag="ACHX5T_002300"
     CDS             complement(7007..7549)
                     /gene="nuoI"
                     /locus_tag="ACHX5T_002300"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR8467479.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7564..8541)
                     /gene="nuoH"
                     /locus_tag="ACHX5T_002301"
     CDS             complement(7564..8541)
                     /gene="nuoH"
                     /locus_tag="ACHX5T_002301"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR8467480.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8538..11264)
                     /gene="nuoG"
                     /locus_tag="ACHX5T_002302"
     CDS             complement(8538..11264)
                     /gene="nuoG"
                     /locus_tag="ACHX5T_002302"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR8467481.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11317..12654)
                     /gene="nuoF"
                     /locus_tag="ACHX5T_002303"
     CDS             complement(11317..12654)
                     /gene="nuoF"
                     /locus_tag="ACHX5T_002303"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR8467482.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12651..13151)
                     /gene="nuoE"
                     /locus_tag="ACHX5T_002304"
     CDS             complement(12651..13151)
                     /gene="nuoE"
                     /locus_tag="ACHX5T_002304"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR8467483.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13154..14956)
                     /gene="nuoC"
                     /locus_tag="ACHX5T_002305"
     CDS             complement(13154..14956)
                     /gene="nuoC"
                     /locus_tag="ACHX5T_002305"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR8467484.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15050..15712)
                     /gene="nuoB"
                     /locus_tag="ACHX5T_002306"
     CDS             complement(15050..15712)
                     /gene="nuoB"
                     /locus_tag="ACHX5T_002306"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR8467485.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15728..16171)
                     /gene="nuoA"
                     /locus_tag="ACHX5T_002307"
     CDS             complement(15728..16171)
                     /gene="nuoA"
                     /locus_tag="ACHX5T_002307"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR8467486.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16802..17740)
                     /gene="lrhA"
                     /locus_tag="ACHX5T_002308"
     CDS             complement(16802..17740)
                     /gene="lrhA"
                     /locus_tag="ACHX5T_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR8467487.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4575 a         5062 c         4676 g         3938 t
ORIGIN      
        1 ttgataattt acatatgaaa aaattttcct tttgttatat tatttctgtt ttcactccct
       61 ctgattattt ttaccaaccc tgggtcactg cattatcgcc aacgccccga cacgtaagtc
      121 ctgagcaatt agaaaaaaca gttcgttatc ttacacaaac tgtgcatcca cgtaatgccg
      181 gcaatattga taatctgaat aggtccgcag agtatataaa agagttcttt gtcagtagcg
      241 gtgccagagt taccgcgcag ggcgtccccg attggctatt acgactctgc gcctggaagc
      301 cagaattacc cttacccggc aatgtcctgg ctttatcccg atcggggaga ctttattgct
      361 gtggtcggca gaatgcagga tatcaacgcc gttcgtcagg taaaagcggc attattgtca
      421 tcccgggatt tatctgttta ttctatgaat gccccagggt ttattcccgg tattgatttt
      481 tctgatcacc tcaattattg gcaacacgat attcccgccg taatgattac tgacaccgct
      541 ttttatcgta ataaacaata ccacttgccc ggtgataccg cagacagatt gaattatcag
      601 aaaatggctc aggtggtaga tggtgttata accttgttat acaacagtaa ataataaaaa
      661 tttctcgagg cgggtccaga aacagcgaca tgtacatttt attttccctc gtctgtttta
      721 agatgcgaaa cataaaattc aatggaggaa aaataaatgg gaatgattgg ctattttgca
      781 gaaattgact cagaaaagat taaccaactg cttgagtcta ctgaaaaacc tttgatggat
      841 aatatacatg acacgctttc aggattacgc cgactggaat cactggatcg aagtgagctg
      901 cgtaaacagt tttccattaa gcgactcaat gaaatggaga tttatccggg agtgaccttt
      961 tcagaagagt tagaggggca actctttgcc tcgatcatgc ttgatatgga aaagctgatt
     1021 agcgcgtatc gacgtatgct gcgacaaggg aatcatgcgc tgacggttat tgttgggtga
     1081 tggtgtcaga gcgccaaaaa aaaccgccga acatgtcggc ggctttctga cttacaaact
     1141 aacagtttac atcagcggca ttgctaaccg cacaatacta atcagcggtt gtggccatac
     1201 acccagcacc agtaccaaca gtgcagaaat cagcaccacg ataccgcccg cgctgtactg
     1261 ccagtttgac ggtgcatcgc gacccggttg ttccggtgcg tgcaggtaca ggctcaccgc
     1321 cacgcgcagg tagtagtaga ggccgattgc cgaaccgaca accacggcac ccaccagcca
     1381 ccacaagtgt gcctggacac cgaccgccag cacgtagaac ttaccgataa agcccagcgt
     1441 catcgggata ccggccagag acagcatcat caccgtcatc actgccgcga ggatcggacg
     1501 atgccagaac agaccgcggt aagagaacag ggaatcagca tccgggccac gatacgggct
     1561 ggacatcagg ctgaccacgc cgaacgcgcc gaggctgctg aacagataac cggccaggta
     1621 aacccctacc gcttccatcg acatctcgcc ggtttgcagc gcaatcagcg ccaccagcag
     1681 atagccgagg tgagagatag atgagtaacc gagcagacgt ttgatgttgg tctggctcaa
     1741 cgccatcagg ttaccgaaga tgatggaggc aaaggcgata atcgccagta ctacgcgaat
     1801 cgcttcgctg tcacccaccg gtgcgtagag gaacagacgc atcaccacac cgaagatagc
     1861 gattttgctc gccgtcgcca ggaaagtgga aaccggcgca ggcgcgccct ggtatacgtc
     1921 tggcgtccac aggtggaacg gcaccagaga gagtttgaag ccgaggccaa caatcatcat
     1981 gccgaaacct gccagcaaca gcggctcgtt gagcataccg tcgccgaggt ttttacccag
     2041 cgcgacaaac gacaggtcgc cagactgcgc atacaccagc gccataccga acagcaggaa
     2101 agaagacgct gcggcagaaa ggatggtgta tttgatactg gcttccagcg aacgtttctg
     2161 gcggaaagcg taaccgacca ggccaaacag cggcaaagag atcagttcaa taccgaggaa
     2221 cagagacgcc agatggtttg cattcgccag caggatcccg cccagcgcgg caattaacac
     2281 caacaggtag aactcatcct tgttgtcgtt atagccttca agccacgggt aggcgaaagt
     2341 acaggtggcg aggctcgcca acaataccag cccggtgtaa agcatggcga aaccatcaac
     2401 gcgcatcagc ggcgtaacgt ccatagcgcc cgcctggcca acaaaccaga gcgaaaccag
     2461 cgccgcgtta agcccaataa ccgagagcgt agcgttgagg aaatgattgc gtcgccacgc
     2521 aatggagagc atcacaacca ccaccgtcaa gccgacgatc agcaacggta gcagtgcgat
     2581 caggttttgt ggagttattg tcatggcgat ttacggcctt gtagtagtaa cggaattaac
     2641 aaaccactgc tggatattgc caatcgcgga gtgcgaggta tccagaatcg gctgcggata
     2701 gaagcccagc agtaccagca gcaccaccag caacaggatc ataaacagct cacgcagcga
     2761 catccctggc agttcctggc tggcaatctg gcttttcgct ttaccgaagt aagcgcgatg
     2821 caacatcgcc agcgaataaa cagatgcaaa gaccagccca aaggtagaga taacggtaat
     2881 caccgggaca acctggaagc tgccgaacag aatcataaat tcgccgacga agttaccggt
     2941 gccaggcatc ccaagcgttg ccaccgcaaa gaacagcgac agtgctggca gccatttcat
     3001 cttgctccac agaccgccca tcatgcgcat gtcgcgggta tggatacgtt cataaagctg
     3061 accgcagaga ataaacagac ccgccgccga caagccgtgc gcaatcatct ggattaccgc
     3121 gccctggtag gccaactggc tgccggtgta gattgcaatc agcacgaagc ccatgtggga
     3181 aaccgaggtg taggcgatca gacgtttgat atcggtctga gcgaaggtca tccacgcacc
     3241 gtagaagatg ccgataacac ccagccacat agcgattggc gcgaactctg ccgacgcgtt
     3301 cgggaacagc ggcagggaga aacgcaacaa accgtaagcg gcggttttca gcaagatccc
     3361 cgcgaggtcc acggaaccgg cggtcggagc ctgggagtgc gcatccggca gccagccatg
     3421 cagcggaacc accggcattt tgactgcgaa ggcgatgaag aagcccagca tcaacagata
     3481 ttccacacca ctggacattg gcgtattcag cagctcttca tagttgaagg tccagacgcc
     3541 ggtcgcattg tagtgaacaa aaaccagcgc caggatggcg atcaacatca ccagaccact
     3601 cgcctgggtg taaatgaaga acttggttgc cgccgtgata cgcgttttac cgtcagaggc
     3661 tttatgcccc cacagtgcga tcaggaagta catcggcacc agcatcattt cccagaagaa
     3721 gaagaacagg aacatgtcga tggcaaggaa cacgccgata acgccgccca ggatccacat
     3781 caggttgagg tggaagaagc cctgatattt ttcgatctct ttccacgaac atagtaccgc
     3841 cagcacaccg agcagaccgg tcagcacgac catcagcagc gacagaccgt caatggcgag
     3901 atgaatagag ataccaaaac gcgggatcca cggcatgtcg aattcagact gccactgcgg
     3961 aattccggcg gattgcgtca gtgaataacc gccctgcaac cacagttgca gcgacagcgc
     4021 cagcgtcaat cccatggtga tcagcgcgat ccagcgtggc accttgacgc caaagcgttc
     4081 ggtctgccag cacaggaagc cgccaataaa gggaattaat attagccagg gtagtaacat
     4141 ggcgatcttt attccttgta aaagtcccgt caggaccgga ttttcaacga attctcacga
     4201 cagaaaattc tcttcggact gggggtagtg tcggatgcgc cgcgaaggca gcttatccga
     4261 cctacgttct tatatgccca ggggcaatcc cacaatcctt aactcaacgc agtaccatca
     4321 acagtgccag caccacgacc gcaccgatgc tcatggatgc cacataccag cgcagataac
     4381 cgttctcgct taacagcaga cctttacctg caaagcggga aagaaccgcc gggatgttca
     4441 tcattgagtt cagcggatcg cgtttcagca accaggcaat acccaggaac ggcttgacga
     4501 acactttgtc gtacagccag tcaaagcccc aagcgttgta ccaccaggta ctcagcaggc
     4561 ggcccggcgc actgttggcg atggaggtca ccagagaacg tttacccagc cacagccagg
     4621 ctgccagcag aatgccgacc accgcgacca cgccagaggt aatttccagg gtcaacatgc
     4681 tgccgtgcgc cagttccgtc gtttgcggaa gcacgccctg cagcggcggt acaatcagtg
     4741 cgccaacgaa ggtggaaagg atcagcagca caatcagcgg caggctgtga gttacccctt
     4801 tcacggcgtg agcgtgaatt tgttcttttc cgtggaagac gatgaaaatc atacggaagg
     4861 tgtagagcga ggtcataaac gcaccgacca gacctgccac catcagattg atatgaccat
     4921 tcgccatcgc acccgcgagg atctcatcct tactgaagaa gcccgcagtg accagcggca
     4981 gtgccgacag tgctgcgccg cccaccagga agcagagata aaccagcgga atagatttac
     5041 gcagaccgcc catcttgaag atgttctgtt cgtgatggca ggccagaatg acggaaccgg
     5101 atgccaggaa cagcagcgct ttaaagaacg cgtgagtcat caagtggaaa atcgccgcat
     5161 cccatgcctg cacgccaagc gcgaggaaca tgtagccaat ctggctcatg gtagagtaag
     5221 cgagaacacg tttgatgtcg gtctgtacca gcgcggcaaa accggccagc agcagcgtaa
     5281 ccgccccgac aatacccacc agatgcagaa cttccggcgt catcaggaac aggccgtggg
     5341 tacgggcgat caggtagaca cccgcggtta ccatcgttgc ggcgtggatc agcgcggaga
     5401 caggcgtcgg gcccgccatc gcgtcagcaa gccatgtctg caacggcaac tgcgcagatt
     5461 taccgaccgc accgcccagc agcatcagcg tcgcccacat cagcatgtta ttgccgtcag
     5521 caaagtgcgc tggtgccagt tccaccatat cgcggaagtt cagggtgccc agttcgttgt
     5581 aaagaatgaa cagtgcgaaa gcgaggaaaa cgtcacccac acgggtcacg acgaacgctt
     5641 tcattgctgc tgcgccattc ttcggatcgg tgtaatagaa accgatcagc agataggagc
     5701 acaggcccac gccttcccag ccgaggtaca tcagcagcag gttatcggcg agaaccagaa
     5761 ctaccatgct ggcgatgaac aggttggtgt aggcgaagaa gcgagagtag ccctcttcac
     5821 cgcgcatgta ccaggaggcg tacatgtgaa taaggaaacc cacaccagtg accaccgaga
     5881 gcatggtcag cgacaggccg tccagcacca ggttaaaacc gatgttaaag tcgcctaccg
     5941 acatccacgt ccacagcggc tggctgtatg cctgctcgcc gttagcgaag aaatcaacgc
     6001 cgataaaggc ggttaccagc gccgccaggc ccacagagcc aacgccgacg atcgccgaaa
     6061 cgttttcaga ccagcgccca cgggagaacg ccagcaggac gaagccaatc aatggcaaaa
     6121 taatggttaa ggcaagcatg ttcatccgcg catctcactt actgaatcga tgttcaggtt
     6181 ctggcgacga cggtgaagtt gcagcagcag cgcaaggccg atactcgctt ctgccgccgc
     6241 gaggctgatg gcgagaatgt acatcacctg accgtcggtc tgaccccagt agcttccggc
     6301 gaccacaaag gccagcgcgg aggcgttaat catgatttcc agaccaatca gcataaacag
     6361 cagattgcga cggataacca gaccggttaa gccaagaacg aataagattg ccgcgaggat
     6421 cagtccatgt tgtaagggga tcatgcgtgc tcctccgttt ttcttttcgc gctgtcgtct
     6481 ttacgattgc tcagcacttc acccgcacgc tcttcacgac cgacgtggaa ggccacaacc
     6541 agacccgcga gcagcagcat agaagccagt tccaccgcca gtacgtaagg cccgaacagc
     6601 gtaataccca ctgctttagc actgattggc gtaccgtcga taccctgatc gttaacaccg
     6661 aggatggcgt aaacaatcac caccagcatg atggccgaca aaattgccgg gccaatccac
     6721 acctgcggtt tcagccactg gcgttcctgt tcgatttctg aaccgcccag gttgagcatc
     6781 atcaccacga acacgaacag caccataatg gcacccgcgt agacgataat ttccagcgca
     6841 ccggcgaagt aagcgcctag tgagaagaac accccggaaa tcgccagcag cgaaataatc
     6901 aggtacagca gtgcgtgtac cggattggta tgggtgatca ctcgcaaggt cgcaagtatg
     6961 gctatcaggc cacagatata aaaagcgaac tccattgccc ctctccttac ggtaacaggc
     7021 tcttgacgtc gataggcttg gcttcgttct ctgcttcgcc cttatctttg ccgtcgattg
     7081 ccatacctgc catccggtag aagttatatt ccgggtattt gcccggaccg gagatcagca
     7141 gatcctcttt ctcgtaaacc agatcctggc gcttgtattc ccccatttcg aaatccgggg
     7201 ttaactgaat cgccgtggtc ggacaggctt cttcgcacag accacagaaa atgcagcgtg
     7261 agaagttaat gcggaaaaat tccggatacc agcgaccgtc tttggtttct gctttttgca
     7321 gcgagataca gccgaccggg caggctaccg cacagaggtt acaggctacg caacgctctt
     7381 cgccgtccgg gtcgcgggtc agaacgatac gaccacgata acggggcggc agatagaccg
     7441 gctcttccgg gtacattcgc gtttcgcgtt tggcgaacgc gtgcaggccg atcatccaga
     7501 tactacgaac ctgggtgccg aaacctacta acaattcttt taaggtcatg gtcttattgc
     7561 cccttattgc gcctgccaga gaatgacagc cgccgttacc agcaagttga tcagcgtcag
     7621 cggcaggcag attttccagc cgaaggacat tacccggtca taacgcggac gcggtaacga
     7681 cgcacgaatc aaaatgaaca tcatcataaa gaacgcggtt ttcagcgcga accagatgaa
     7741 tggcggtaac aacgggcctt gccagccacc gaagaacagc gtcaccatca atgcagagat
     7801 ggtcacaatc ccgatgtact cacccacgaa gaacagaccg aacttcatac cggaatattc
     7861 aatgtggtaa ccatccgcca gttcctgctc ggcttccggc tggtcaaacg ggtgacggtg
     7921 acacaccgcc acgcccgcga tggcaaaggt aataaaacca aagaattgcg ggatgacgtt
     7981 ccacacatgc gcctggctgt tgacgatgtc ggtcatgttg aatgaaccgg cctgcgccac
     8041 cacgcccatc agggaaagcc cgaggaacac ttcgtagctc agggtctgcg cagaagcacg
     8101 catcgcaccc agcagcgagt atttgttgtt acttgaccag cccgcaaaca gcaccgcgta
     8161 aaccgccaga cctgccatca tcaggaagaa caaaatcccg atgttcaggt cggcaaccac
     8221 ccaacccgga ctgactggca caattgcaaa ggccagcagc agcgaggtaa aggcaatcat
     8281 cggtgccagg gtaaagatga cgcgatccga gaatttcggg atccagtctt ctttaaagaa
     8341 cattttgatc atgtccgcaa ccagctggag cgaaccgccc cagccaacac ggttaggtcc
     8401 ataacggttc tggaacagac ccagcaggcg acgttcgcca aagctcatga atgccccaca
     8461 ggtgacaacc accagcagga tcaccaccgc tttgaggatg gtcagcagga tctcaatcag
     8521 ttccggtgat atccaactca ttgttgtgcc tccttgagat cctcaagatg cgcgccagcc
     8581 agcaccggag caatgccgga catacccatc ggcaagccca cctgccctgc cgtcagtcct
     8641 tcggcgattt caaccggcag cgtgaccgtg ttgccatcgt aactaaagga gacgcgtgta
     8701 cctgcgttca cacccaactt cgcggcatcc gctgggttga gtttgatgta cggctgcggc
     8761 atacggctct ggaagaccgg agcacgctgt gacaattcat cgctgccaaa caggtggtag
     8821 tacggcgcga tacgccattt cccgtcctgc ggctggaagc gtgccggtac gctggtgaag
     8881 taatccagac cgttttcgct ggtttcaaac agacgcacgc ccggatcgcc aaagcgcagt
     8941 ttgccgccca cttcgtcctg gaatttgttc cacgcctgcg gggagttcca gcccggcgcc
     9001 caggcaaacg gcacttgcga acggtgcgcg gtcggctggt tgttaccttc catcgagaag
     9061 gtgaacatgg tgtcaatatc ctgcggctga cgcggctcat gaacgctgat attggcgcgc
     9121 atggcggtac gaccgctgta acggtgcggt tcacgggcca gtttctgccc acgaatacgg
     9181 aatgtcgcat ccggcgcagc atctttgata cccgccagtt ctgggatttt cgccacaaca
     9241 gcgtcaatta catggtcgag ctgcgtccag tccacttcac ggctcagcaa ggtgctgtgc
     9301 agcgagtgca gccagcgcca gctttccagc atgacagttt tgctgtcgta ataagccgga
     9361 tcgtaaacct ggaagaaacg ttgggcgcga ccttcattat taattaccgt accgtcgctt
     9421 tcagcaaagc tggcagagga aagcaccaga tgggcgtttt ccatgatcgc tgtgcgttga
     9481 tgatcaacca ccatcaccag cggcgcttta gccagcgcag cattcacgcg ggtagcagaa
     9541 gcgtaacgat gcagatcgtt ttccaacacc accaccgcgt cggcgcgtcc ggtttccagt
     9601 tcggttaacg cttcttcaag cgaaccgcca cccataatgc ccagccccat gctgttgacg
     9661 gaacgggcaa tcatggtgat accaacgtca gcgccgcgac ctttcagggc tttcgcgacg
     9721 ttagccgcag cctgaatcac ctctatgcta ccggcgttcg tcccggagat aatcaacggt
     9781 ttcttcgcac ctgccagtgc ctgcacgatg acgtcgattt tgttttgcag ctcaggttcg
     9841 ataccgtcaa ccgctggtgc ggagttatcc agcgcatggg caatggcaaa acctaaacgc
     9901 gcctgatctt caaccggtgc gcggtaagtc cacgccgcga tatcatccag acgagtgtca
     9961 tcaacgttgg taacaaacag cggatgcttc gcacgttgac cgatgttgag gattgccgca
    10021 atctgccagt cagccacttt ctgtgctgca gccatttcgc gcgctttacc tttcaccgcc
    10081 tgacgcactg ccagtgcgac gcgcgcgccg gtctgggtaa cgtcttcgcc cagcaccagt
    10141 accgcatcgt aagattcgat ttcgcgcaga gccggagtat aaatgccgcc ttcacgcagc
    10201 actttcagcg ccagttgcag acgttcctgc tcaccgtgag cgataccggt gtagaagttt
    10261 tcttcgccca ccagttcacg cagcgcaaag ttgctttcca cgctggcacg cggagaacca
    10321 ataccgatca ctttcttcga ctgacgcaga atatctgccg cgccctgcat tgcctgttcg
    10381 gcgttgaggg taatgaaatc atcgccacga cgctgtactg gctgacgcgg acgatccttc
    10441 aggttgacgt aaccgtaacc gaaacgacca cggtcgcaga ggaagtagtg gtttaccgta
    10501 ccgttgtaac ggttttcgat acgacgcagt tcgccgtaac gttcaccggg gctgatgtta
    10561 cagccgatgg aacattgctg gcagatgctc ggcgcaaact gcatatccca tttacggttg
    10621 taacgctcgg agtgcgtttt gtcggtaaat acgccggtcg ggcaaatttc gaccaggtta
    10681 ccggaaaatt cgctttccag cgtgccgtct tccgggcgac cgaagtagac gttgtcgtgc
    10741 gcgccataaa cgcccagatc tgtaccgtca gcgtaatctt tgtagtaacg cacacagcgg
    10801 tagcaggcga tgcagcggtt catttcgtga gagatgaatg gccccaaatc ctgattacgg
    10861 tgggtacgtt tggtgaaacg gtagcgacgg aagctgtgtc cggtcatcac ggtcatatcc
    10921 tgaagatggc agttaccgcc ttcttcacat accggacagt cgtgcgggtg gttggtcatt
    10981 aaccactcga ccacactttc acggaactgt ttcgcttctt cgtcgtcaat ggaaataaag
    11041 gtgccatcgg aagccggtgt catacaggac atcaccaggc gaccacgcgt gtcttccgcg
    11101 ttttggtatt gcttcaccgc acactggcgg caagcaccga cgcttcccag cgccggatgc
    11161 cagcaaaagt aaggaatatc aaggcccaga gacagacaag cttccagcag gttgtccgct
    11221 ccgttgacct cgtattcttt gccgtctaca tgaattgtag ccattagcat gcttccagtt
    11281 ttctcggtca gggaccgagc gttaatcgaa attcggttac cagcgctctt tcagcaggtt
    11341 cggctgaatc ccattaatca aatgggtatt gctgaacggc tgtttgattc ccgcctcaaa
    11401 ttcttcgcgg aaatatttga tggcgctctg taacggctcc actgcaccag gtgcgtgggc
    11461 acagaaagtt ttacccgggc ctaagaatcg acacagttgc tcaagtgttt cgatatcgcc
    11521 cggctggcct tcgccacgct ccagcgcacg cagaattttc acgctccacg gcagaccgtc
    11581 gcggcacggc gtacaccagc cgcaggactc acgggcgaaa aactcttcca ggttacgcac
    11641 cagcgacacc atgttgatct catggtcaac cgccatcgcc agcgccgtac ccagacggct
    11701 gcccgcttta ccgatacttt cgaattccat cggcagatca aggtgcgctt cggtcaggaa
    11761 gtcagtcccc gcgccgcctg gctgccaggc tttaaatttc agaccatcac gcataccacc
    11821 ggcgtaatct tcgaggatct cgcgtgcggt ggtgccgaac ggcagttccc acagtcccgg
    11881 atttttcacc cgaccggaga agcccatcag cttggtgcca gcatctttac ttttcgagat
    11941 gttctgatac cactccacgc cgttagcgag gatcgccgga acgttacaca gggtttcgac
    12001 gttgttgaca caggtcggtt tgccccatac gccggaggtt gccgggaagg ggggtttcga
    12061 gcgtgggtta gcacgacgcc cttccaggga gttgattaac gctgtttctt ccccgcagat
    12121 gtagcgccct gccccggtat ggacgaacag ttcgaagtcg aaacctgttc ccataatgtt
    12181 tttgccaagc aagcccgctt cagtggcttc ggcaatggcg cggcgcagat ttactgccgc
    12241 ttcgatatat tcgccacgca ggaagatgta gccacggtaa gctttcagcg caaacgcgga
    12301 gatgagcata ccttccacca gcaggtgcgg cagttgctcc atcaacaggc ggtctttata
    12361 ggtgcccggc tccatttcat cggcgttaca cagcaggtaa cggatgttca tggattcgtc
    12421 tttcggcatc aggctccact tcaggccagt cgaaaagccc gcgccgccgc gccctttcag
    12481 accagcgtct tttacctgat taacgatttc gtccggagac agcccggtca gcgccttacg
    12541 cgccccttcg taaccgtttt tgctgcggta ttcgtccagc cacactggct gtttgtcatc
    12601 gcgcagacgc caggtcagcg gatgcgtttc gggagtacgg ataatgtttt tcatttatac
    12661 cgctccagca gttcagggat cgcttccggg gtcagatgcg cgtgagtgtc ctcatcgatc
    12721 atcatgtttg gccctttatc acagttcccc aggcagcaag ttggcagcag cgtaaagcgg
    12781 ccatcaaatg tcgtttgccc tggtttgatg ttcagctttt tctcgagcgc cgcctgaata
    12841 ccctgatacc cgttgatatg acagaccacg ctgtcgcaat aacggatcac atggcgacca
    12901 accggctggc ggaagatctg actgtagaac gttgccacac cttcgacgtc gcttgccgga
    12961 atacccagca catcggcgat cgcgtggatc gcaccatccg gcacccagcc acgctgcttc
    13021 tgaacgattt tcagcgcttc aatggacgcc gcacgcgggt cttcgtagtg gtgcatctcg
    13081 tgctcaatcg cttcacgctc tgccgcactc agctcaaaag cctcggtttg tggttgttga
    13141 ttctcgtgca taattagcgg tccacatctg acataacaaa atcgatactg cccagataaa
    13201 caatcaggtc agacaccagg ctgccgcgga tcgccgccgg aatttgctgc aaatgcgcat
    13261 aactcggcgt gcggatacga gtgcgataac tcatggtgct accgtcgctg gtcaggtagt
    13321 aactgttgat ccccttggtc gcctcaatca tctggaaaga ttcattggca ggcatcaccg
    13381 gcccccacga cacttgcagg aagtgggtga tcagggtttc gatatgttgc agcgtgcgct
    13441 ctttcggcgg cggcgtggtc agcgggtgat ccgctttgaa cgggccttcc ggcatgttgt
    13501 tgaggcactg ctcaagaatg cgcagactct ggcgcagctc ttccactttc agcattacgc
    13561 gggtgtagca gtcagaaaca ccaccaccaa ccgggatttc aaagtcgaag ttttcatagc
    13621 cagaataagg acgcgcctta cgcacgtcga agtcgatccc ggtagcacgc aggcccgcgc
    13681 cagtggtgcc ccactccagc gcctctttcg cgccataggc ggcaacgccc tgggaacgac
    13741 ctttcagaat ggtattttgc agcgccgctt tctcgtaaga cgccagacgt ttcggcatcc
    13801 agtcgaggaa ctcacgcagc agacgatccc agccgcgcgg caggtcgtgc gctacgccgc
    13861 caatacggaa ccacgccggg tgcatacgga aacccgtgat cgcttccacc agatcgtaaa
    13921 ttttctgacg atcggtaaag gcgaagaaca cgggcgtcat tgcgccgacg tcctgaataa
    13981 aggtcgagat gtacagcagg tggctgttga tacggaacag ttcggagagc ataacgcgaa
    14041 tgacgttaac gcgatccggc acggtgatcc cggccagttt ctctaccgcc agcacgtaag
    14101 gcatttcgtt aacgcagccg ccgaggtatt caatacggtc ggtatacgga atgtagctgt
    14161 gccaggactg gcgctcgccc attttctccg caccacggtg gtggtagccg atgtctggta
    14221 cgcagtcgac aatctcttcg ccgtcgagtt gtaaaacgat acggaaagcc ccgtgcgccg
    14281 acgggtggtt cggaccgagg ttgaggaaca tgaagtcctc attttcggtg ccgcgtttca
    14341 tcccccactc ttccggtttg aaggtcaggg cttccatctc cagatcctgt ttggctttgg
    14401 tcagctcaaa cggcgagaat tcggtagcgc gtgccgggta atctttacgc agcgggtgac
    14461 ctttccaggt ttgcggcatc atgatgcggc gcaggttcgg gtgaccgtcg aaagtaatgc
    14521 caaacagatc ccaggtttca cgctcatacc agttagcgtt cgggaacagt ttggtgaagg
    14581 tcggtacgtg caggtcgttt tctgccagcg ccaccttcag catgatgtcg cggttacgat
    14641 cgatagaaat cagatggtag aaaacggaaa aatccgcggc aggtaaccct tcgcggtgtg
    14701 tgcgcagacg ttcgtccatg ccgtgtaagt caaacagcat gacgtaaggt ttcggcagtt
    14761 tctttaagaa atcgccaact tccagtaatt gttcacgctt gatccacaca acgggaaccc
    14821 cggtgcgagt cgcctgaaca gtaaaggcat ccggcccaaa acggttgcgc agttcgccaa
    14881 ttaccggatc atcaagatga tcgcgggtct gccaggcggg ttcttgcgcg gttaagtcag
    14941 tcatattgtt caccattgca aatggtccgt ggtgactgtc ggcgcagtaa gcttcgcgaa
    15001 atagatttag gaataagcga agaaaaatcc ctttgccgac aggcgcaaat taaatctcgt
    15061 caggtgtacg caggttggtt acggcaatgc gttcaccgcg cttgcgttcg cgctctgatt
    15121 gcatattggc gcgataaacg ccctgatcgc caaccaccca ggagagcgga cgacgttctt
    15181 tgccgataga ttcctgcaac agcatcagtg cctgcatgta cgcttcagga cgcggcgggc
    15241 agcccgggat atacacatca accgggatga atttatcgac gccctgcaca acggaataaa
    15301 tatcgtacat accaccagag ttggcacagg cacccattga gataacccat tttggttcca
    15361 gcatctggtc atacagacgc tgaataaccg gtgccatttt ggtaaagcag gttcctgcaa
    15421 ccaccatcag gtcagcctga cgcggcgaag cacgcaatac ttctgcgcca aaacgcgcca
    15481 cgtcatgcac cgcggtaaac gaagtcacca tctcaacgta acagcaggaa agaccgaagt
    15541 tatacggcca aattgagttt ttacgacccc agttaaccat gtcattgagc ttgcccataa
    15601 acacgttttt gttaacttct tgctccagag ggtcggttac gatctcctgc ttttgcaggg
    15661 ggtaacggtc gttctcaccg ttgggatcta tgcgggtgag cgtataatcc atcttaatgc
    15721 ctcgcggtta gcgttgacga ttagcgatac tgttcgtttc cgggttcata cgctcgcggc
    15781 gtgaacgcgc gggcgtccag tccagcgcgc caatacgcac cagataaacc agacctgcca
    15841 gtaacacaaa aataaaaatt gcagcttcca caaagcctac ccagccgctt tcgcggatag
    15901 aggttgacca tgcgaacaga tacagcgctt caacgtcgaa gataacgaag aacatggcca
    15961 ccagataaaa cttggcggac aggcgtaagc gggcggagcc gaccgagtcg ataccggatt
    16021 caaacggcac gtttttcgac ctcgcgcgtg cgcgaccgcc taaaaaccaa ccgcctacca
    16081 gcatcaggca acacaggcca atggcaacga taagaaagat agcgaatgcc cagtgatgag
    16141 cgatgacttc agtggatgtt gacatactca ttgcttactc atcaaaagta gcgccagatt
    16201 cactgctctt cacggcagat ggacgccaca tcgattcatg gggaggaata aaaaaaacct
    16261 tacaataact gtagaaattc ttttatacag ctaattgatg tggtctttta ctcctttcta
    16321 taaccttttg tcaactttaa caaaagcttc ttcacattag tttacataat atcaacacca
    16381 ttagcattta atgccctttc accccagatc cttgacgact ccaggataat tagatgttgt
    16441 tgaatcgtgt ccgttgtgaa gcaatggaaa aaatacgggt ctattttgac aggaatttgt
    16501 gtcgattcct ccccccaaaa gagagtattt tcttgatctg tgacacgctt ttgttattcc
    16561 ataacaaaaa cgcagcaaca aatttacgta ttttttaaca tcattgtagc aggtgatttt
    16621 tttcaggcga ttatttgtgc attcgggacg tgaatctctg gtgattgaaa aataaacagt
    16681 tttgtacgtt ctgcactatg cgaggaagga tttttactaa aaaaaagccg ctggggttta
    16741 aaacaccccc agcggctcgt tttttacact attgtctcag gaattatcta tcgtccgtcg
    16801 attactcgat atccctttca atcaacaatg aatcatcccc ttccggagca gacattgggc
    16861 tgtattgcca cggattatgg tagctctcca tcgcctgata gatcacctgg gccagctcat
    16921 tattactgga cgggtcatag cacagcaaat attcggtatc aggtagcggc ggtaagccat
    16981 ctactccact cagcacacgc aggtccgggc tcatcatctc aaccggcctt gccgtcacgc
    17041 caagaccggc tttcactgcc gcacgaacgg ccggaagcgt cgaggcgaca taagccagtc
    17101 gccatggaat atctacttta ttaagcgtcg ccagcaccat atcgcgaaac ggacttggat
    17161 catccagcaa tacaagaggg atcggctcgc ctttttgcaa tacgtattcc gctgcgcagt
    17221 accagtgtgt tggcgaggta cgcaggttca atgccttaaa cgctgaagga cgatgggtgg
    17281 ttaccatcaa atccacttcc tgggattcca gcatttctgc catataggca ttacgtttaa
    17341 cgcggacatc cagcgctaat ttcggataaa ccgaactcac gcgatttaac aggaaaggta
    17401 agatcgtatc ggcagattca tctgaagcac cgatagttaa cacgccctga agattactga
    17461 acattaatga tgagcaggcc tcatcattaa aacgcaggat tttcctggcg taaccaagaa
    17521 gttgaatgcc atgttcagtt aacagtttgt tgcgaccgtg acgagcgaac agttctttcc
    17581 caacgagttg ttccagacgc tgcatttgct gacttacggc ggactgagta cgacacacag
    17641 cggcagctgc ggcagcaaaa gtgttcagat cggcaacagc aacaaatgtt ctcagcagat
    17701 cgaggtcgag gttaattatc ggacgatttg cacttatcat atattatcac ttactggcgg
    17761 ctcatactga gctggttaat gctgtgcaca cacaaacaag caattccatt tgtaatgtgc
    17821 ctccctggca gtttcatccg gaacccggac gaaagtaaaa atgcatatga gttgcactaa
    17881 aaaagcgact cacattgttc cgttataatg cctgaagtag atcacagaat atatcttcag
    17941 ggatcgcata tctattaagt tactcactct tttctattta tgacatgcgc gtgtttgtat
    18001 aaatgtaaat gtgagtcctt gttccactct cgtgcagcat cgctggtcat acgcgaacac
    18061 gtacaaacag caatggtgaa gcggcatcaa gagcagggat ccgtcattta tgcgagcatt
    18121 ttaccccaaa aaccttttat ttataagggt cattgcgaat tatctgatgc aaagttatgt
    18181 tatgttaggc aaagtaatct tctattatta ataagcacat caaaaccttt ttgaatatta
    18241 aataataatt a
//
LOCUS       DAZSIO010000074        18197 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZSIO010000074 DAZSIO010000000
VERSION     DAZSIO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18197
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.074"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..793
                     /locus_tag="ACHX5T_002309"
                     /pseudo
     CDS             1..793
                     /locus_tag="ACHX5T_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(853..2151)
                     /gene="eno"
                     /locus_tag="ACHX5T_002310"
     CDS             complement(853..2151)
                     /gene="eno"
                     /locus_tag="ACHX5T_002310"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR8467488.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="ACHX5T_002311"
     CDS             complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="ACHX5T_002311"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR8467489.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="ACHX5T_002312"
     CDS             complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="ACHX5T_002312"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR8467490.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4991..7225)
                     /gene="relA"
                     /locus_tag="ACHX5T_002313"
     CDS             complement(4991..7225)
                     /gene="relA"
                     /locus_tag="ACHX5T_002313"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR8467491.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="ACHX5T_002314"
     CDS             complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="ACHX5T_002314"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR8467492.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8631..11387
                     /gene="barA"
                     /locus_tag="ACHX5T_002315"
     CDS             8631..11387
                     /gene="barA"
                     /locus_tag="ACHX5T_002315"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR8467493.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="ACHX5T_002316"
     CDS             complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="ACHX5T_002316"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR8467494.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12980..14320)
                     /locus_tag="ACHX5T_002317"
     CDS             complement(12980..14320)
                     /locus_tag="ACHX5T_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR8467495.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="ACHX5T_002318"
     CDS             complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="ACHX5T_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR8467496.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16108..16557)
                     /locus_tag="ACHX5T_002319"
     CDS             complement(16108..16557)
                     /locus_tag="ACHX5T_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR8467497.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16575..17357)
                     /gene="truC"
                     /locus_tag="ACHX5T_002320"
     CDS             complement(16575..17357)
                     /gene="truC"
                     /locus_tag="ACHX5T_002320"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR8467498.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17357..17686)
                     /locus_tag="ACHX5T_002321"
     CDS             complement(17357..17686)
                     /locus_tag="ACHX5T_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR8467499.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
BASE COUNT         4474 a         4920 c         4324 g         4479 t
ORIGIN      
        1 tgttgcagcc caaccaacta ttgtcccaca attacagcaa caagttacgg gtcttacgag
       61 tagcttaaac tcacaagaaa agaaagaact gacgcacaag ttagaatcta ttttcaataa
      121 tacccaagtg caaattgctg tattaattgt ttccacaacc aaagatgaga caattgaaca
      181 atatgctaca agagtttttg acaattggcg tttaggagat gccaaacgta atgatgggat
      241 actgatcgtt gttgcctagt cggatcgcac tgtccgcatc caggtaggtt atgggctgga
      301 agaaaaggta accgatgctc tggctggaga tatcatccgt agcaacatga tacccgcatt
      361 taaacaacaa aaattagcta agggattaga gttagctata atcgctttga ataaccaact
      421 cacttcacaa caccaatatc cggctaatcc ttcagaaagt gaatcaacgt cttccactga
      481 tcattattac tttgctattt tttgggtatt tgcagtgatg ttcttccctt tctggttttt
      541 tcatcaaggc agtaattttt gtcgcgcatg taaaagtgga gtctgtattt cagcaatcca
      601 tcttttagat ttattcctgt tctccgataa aactttttcc attgctgtat tttccttctt
      661 ttttactttc accatattta tggtctttac ctgtttatgt gtacttcaga aaagagcatc
      721 tggtagaagt tatcattcag acaacagcgg ttccgctgga ggctcaagtg gcggcgcatc
      781 cggccgctgg taactccacc ataaaaaatg ccagcccgaa ggctggcatt atcaaatcag
      841 ataaagtcag acttatgcct ggcctttgat ctctttacga ccgttgtacg gtgctttttc
      901 gcccagagct tcttcgatac gaatcagctg gttgtattta gcaacacggt cagaacggct
      961 catagaacca gttttgatct ggcctgcagc agtaccaaca gccagatcag cgatggtagc
     1021 atcttcagtt tcaccagaac ggtgagagat aactgcagtg tagccagcat ctttcgccat
     1081 cttgattgca gccagagttt cggtcagaga accgatctgg ttgaatttga tcaggatgga
     1141 gttagcgata cctttttcga taccttcttt caggatcttg gtgttggtta cgaacaggtc
     1201 gtcaccaacc agctggattt tgtcgcccag aactttggtc tggtatgcga aaccgtccca
     1261 gtcagattcg tccagaccgt cttcgataga aacgatcggg tactgtttgg tcagttcttc
     1321 caggaagtga gtgaattctt cagaggtgaa cgctttgttg ccttcgccag ccagaacgta
     1381 tttaccatct ttgtagaatt cagaagctgc gcagtccatc gccagagtga tgtctttgcc
     1441 cagttcataa ccagcagctt taacagcttc agcgataaca gccagagctt cagcgttgga
     1501 acccaggttc ggcgcatagc caccttcgtc accaacagca gtgttcatgc ctttcgcttt
     1561 cagaactttt gccaggtgat ggaaaacttc agaacccatg cggatggctt ctttcacagt
     1621 tttcgcgcca accggctgaa tcatgaattc ctggatatca acgttgttgt cagcgtgctc
     1681 accaccgttg atgatgttca tcatcggaac cggcatggag tatttgcccg gagtgccgtt
     1741 cagttcagcg atgtgctcgt acagcggcat acctttagca gctgcagcag ctttggcgtt
     1801 agccagagat acagccagga ttgcgttcgc gccgaatttg gatttgtttt cggtgccgtc
     1861 caggtcgatc atgatcttgt caatgccagc ctgatcttta gcatctttgc caatcagcgc
     1921 ctgagcgatc gggccgttta ccgcagcaac agctttggtt acgcctttac ccaggaaacg
     1981 ggatttgtcg ccatcgcgca gttccagagc ttcacgggaa ccagtagaag cacctgacgg
     2041 agcagctgcc ataccgacga aaccaccctc cagatgtact tcggcttcaa cagtcgggtt
     2101 accacgggag tcgatgattt cacgaccgat gatttttacg attttggaca ttaggttttc
     2161 ctcaagtcac tagttaaact gaaactccag acaaacaacg cgtacccaag gtacgcgttg
     2221 ccgttctaac ttttttactt acttcgcctg acgtttctgg aactcgctgg cggctttcac
     2281 aaagcctgca aacagcgggt gaccatcacg tggagtagaa gtaaactccg gatggaactg
     2341 gcaagccacg aaccacgggt gattcggaac ttcgatgatc tcgaccaact gatcatcccc
     2401 ggaacggccc gcaacgcgca gacctgcatc ttcaatctgt ttcaacagca tgttgttgac
     2461 ttcgtaacgg tgacgatgac gctcaacaat tgtcggcgca ttgtatagct ggcgaaccag
     2521 gctatcgtca accaactggc actgctgtgc gccgagacgc atggtaccgc cgagatcgct
     2581 cttctcgcta cgaacttcaa cgttgccgtt ttcatcgcgc cactcggtaa tcagcgccac
     2641 aaccgggtac ttacagtctg gcacaaattc tgtagagttg gcgttctcca tgttggcaac
     2701 atggcgagcg taatcaatta acgccacctg catacccagg caaatgccca gataaggaat
     2761 attgttctca cgcgcaaaac gcgcggtcgt aatcatgcct tctacgccac gatagccgaa
     2821 accgccaggt acgaggattg cgtccagacc tttaaggatt tcaacgccgc gcgtttcaac
     2881 atcttgtgaa tcgatcagtt tgatgttgac gctgacacga ttcttcagcc caccgtgttt
     2941 cagtgcttcg atcactgatt tataagcatc cggcagttca atgtacttgc cgaccatacc
     3001 gatggtgact tcacttaccg gattcgcttc ttcgaagata acctgttccc attcggacag
     3061 atttgcttcc gggcagttta agctgaatcg tttacaaata taatcgtcca gcccctgaga
     3121 tttcaacagg cccggaattt tatagatgga atcgacgtct ttcagagaaa taaccgcttt
     3181 ttccggaaca ttacagaaca atgcaatctt cgcacgttcg ttcgccggta cagcgcgatc
     3241 tgaacgacaa atcaggatgt caggctggat accgatggag agcagctctt ttacagagtg
     3301 ctgagtcggt ttggttttga cttcaccaga cgctgccatg tacggcacca gcgtcaggtg
     3361 cataaacaga gtgtgctcac ggccaatttc aacagccatc tggcgaatag cttcgaggaa
     3421 cggcaaggat tcgatatcac ctactgtacc gccgatttct accagtacta cgtcatgacc
     3481 ttcgccacct tccagcacgc gctctttgat tgcgttagtg atgtgcggaa taacctgcac
     3541 ggttgcgccg aggtagtcac cgcggcgttc tttacgcaga acgtcagagt agatacgacc
     3601 cgtggtgaag ttgttgcggc ggctcatttt ggtacgaatg aaacgctcgt agtgccccag
     3661 gtccaagtcg gtttcagcgc cgtcttcagt aacgaacact tccccgtgtt ggattgggct
     3721 catagtacct ggatcgacgt tgatgtaagg atccagtttc atgatggtca cattgaggcc
     3781 acgggcttca agaatggctg cgagggaggc tgcggcaatg cctttaccca gagaggatac
     3841 gaccccgccg gtcacaaaaa tatagttcgt tgtcatgctg aacctgagaa gttaggttga
     3901 aaagacgatg gaataaccaa gacgggaaag cagtataccc gaacatgacc tgtgccacaa
     3961 actttcatta tccctcctct tcgccagcgc actattgaaa tcaggagtga gaaaatagcc
     4021 cctttggggt aaatgttttt gacgcaaatc aagcgcttgt catttaaaaa atgacacaaa
     4081 tggcgcttga ccgcgtaatt cccttagaga tcaatttcct gccgttttac ctgttgccag
     4141 acttcttcca ttgtttcgag gtcaacacct gtcatttcca gtccacgcgc ggcaacaata
     4201 cgctccactt cgcgaaaacg acgctcgaat ttttcgttca ctttttgcaa tgcgatttct
     4261 gctttcgtcc ctaaatggcg agccagatta accgtggcaa acagcaggtc ccccatttcc
     4321 tcctccagtt tagcctggtc gacaacagcc tgccgcgcct cgtacatcac ctcgtcgatc
     4381 tcttcgtaga ctttatcaac caccgggcca agcgtcgtcc agtcgaagcc aacgttggcg
     4441 caacgtttct ggattttttg cgcccgcatt aaagccggta aactacgagg aatatcgtcc
     4501 agcgccgaat gctgcgcttt ctgcgcgcgc tcttcggttt tgatttgctc ccaacgggca
     4561 agcacttcac tactgttttc ggcagaacta tcagcaaaaa catgcggatg gcgacgctct
     4621 aatttatcgc taatagcagc gcaaatatca ttaaagtcaa agcgcccttc ttcctgagcc
     4681 atttgcgcgt aaaacaccac ctggaatagc agatcgccca gttcaccgcg aagatcgtca
     4741 aaatcttcac gggcgatggc gtccagcact tcgtaggttt cttcaagggt gtaaggcgca
     4801 atggtggcaa atgtctgctc tttatcccac gggcagccgt tttccggatc gcgcaggcgc
     4861 tgcataatag tgagcaaacg gtcgatttga ttcattgaat tgtcctgaaa attgcgggtc
     4921 tgtcaggtgt aataccttcg agtaaaagat atcgctggat acggctgacg ccatatccag
     4981 ctatgtaaga ctaactcccg tgcaaccgac gcgcgtcgat aacatccggc acctggttga
     5041 gtttacccag cacgcgcccc agcacttgca ggttgtaaat ctcaatggtc atgtcgatgg
     5101 tcgccagttg ctgtttggtg tcgctacggc tggcaacgcc aagcacgttc accttctcgt
     5161 tggcgagaat ggtcgtgata tcacgtaaca acccactacg atcattagcc accacgcgga
     5221 ccaccagcga atatccggcg gagtagctct caccccatac cgcgtcaaca atgcgttctg
     5281 gcgcatggga gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact gaaataccgc
     5341 gtccctgggt aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga
     5401 tgtggtgcat caggttacca acaccttcaa ccaccacacg accgttatct ttactgcggt
     5461 tttgcggtgt gtagcttttt tgctgaagct gtttcagcgc ggcggcgtcc tgctcttcgg
     5521 cactcggctt attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc
     5581 cgccaccaat cgccgccagc aactcgtcga catcattgaa gttgtaacgc ggcagcagat
     5641 gtttttctgc ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc
     5701 gcccagccag aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac
     5761 gcccacggct ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg
     5821 gctgtttctg ggtgataatt tcaatctggt cgcccatttg cagctggtag gtgaacggca
     5881 caatgcgccc gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt
     5941 aagcgaagtc cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa
     6001 agacatacac ccggtcgtca aacacctgac tacgtacttc gtcgagcatt tcgccggaat
     6061 cagccatctc ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc
     6121 ccgaacgtgc gccgccagct gccgcgccct ctttatattt ccagtgcgca gcaacaccca
     6181 actccgcatc ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt tttccacccg
     6241 gccccagaac cacggtatga atagactgat aaccgtttgg tttcgggtta gcgacgtaat
     6301 cgtcaaactc atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcataac
     6361 aatcctgtaa acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcgaagg
     6421 cgaggttctt tttctgcatt ttgcgccaga tgctgtagat gtgtttcgga cgaccataca
     6481 cttcagcttt aacgccttca gctttcatct cagcgcgcag atggccgaca aactcttcga
     6541 tatagtgttc gcggtcgaga cggcgttcat gcagcagttt tgcgatgcgt ttgtattcgg
     6601 tcgggtggag ataacggaag cagtaatctt ccagctccca tttcagttgc ccaatcccaa
     6661 gacggttcgc caacggcgcg tagatattgg tgcactcttt tgccgccagt acacgttcat
     6721 cttccggcgc atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacta
     6781 cgcagcgaaa atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg
     6841 aggaaacaga atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc
     6901 cgtgaataag gttaacaacc gacttaccga cgctctcacg cagcacatct tcgctgacta
     6961 cgttggcatc agccagaggg aacagcagcg ccgcccgcag cgtgtcaatg tccatactta
     7021 atgtcgagag gatctccacc atctcaacac cacgccacaa taacagactg gcatccggat
     7081 gcccctgcgt ctgttgcaga caatacgccc aggtttcggc taagcactca cacgacttct
     7141 ggctggtaat acccagactt gcgatccatt tttccggatc aaattcacca gccttattga
     7201 tatgtgcact tcttaccgca accatcgtcc tctcctttag ggaccagacc tgccgaaatc
     7261 ggcaaatcgt aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg
     7321 tgggaacata tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc
     7381 gctatcccga gccagcgttg cagggttaca ggatacataa actatacgaa taggttccag
     7441 ttttataatt tgctgcataa cacctgcggc acctgctcgc gccgggtcca gcaacacttt
     7501 atcgaagccg tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcgtgata
     7561 aaacgtcaca ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc
     7621 aggaacacct tctacaccga ccacactggc agcttgtgtc gccaatggca gtgtaaagtt
     7681 gcccataccg cagaacagat ccagtacgcg atcttcaggt tgcacatcca gccattccaa
     7741 cgcacgcgct accatttttt ggttcacacc cgcattgacc tgaataaaat cgcgcgggct
     7801 aaaagttaag cgcaacccgt ttgagtcata ccagggcatc tcaccagaga cggtttcgag
     7861 tatctcacta tcgggggcga gatacagatc caggccttca gaatgcgaaa agcgttccag
     7921 tttttcgcga tctgccgaac ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct
     7981 ggttgcctgt accagttcaa catgaccaag atggcgcata gcttgtaagc tgcccagaca
     8041 tgccctgact ttgggcagca atgcttcaag ttggggcgct aaaatggggc attgtttgac
     8101 gtcgacaatg tcactggagc ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg
     8161 taagtagttc agacttaaac gcgcgcggcg gcgatagccc cagggaacat cggcgatcac
     8221 ttcagagaca tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa
     8281 atccacgctg gcgtgttgtt gctgacagcc gccgcatacg ccaaaatgag gacagcgtgg
     8341 cgtttcgcgt tccgggctat cgcttaaccg gcgtacgact ttagcgcggg catactgttt
     8401 tttatcttca gtaacagcaa cttccgcgtt ttcctgcggc aataatccgg ggataaataa
     8461 cgttttgccg ttatgtcgcg ccacgccctg accaaaagag tcgaggtcgt tgactgaaac
     8521 ggttatgatc tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga
     8581 gactttctca atttaacagt gtgaccttaa ttgtcccata acggaactcc atgaccaact
     8641 acagcctgcg cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat
     8701 tgctgagtat ctttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg
     8761 ccggtgctag cattattgag ccgcttgcag tttctactga atatggcatg agcctgcaaa
     8821 atcgcgaatc tatcggtcag ttaataagcg tactgcatcg tcgccattcc gatattgttc
     8881 gcgcgatttc ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg
     8941 atccctcatc aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc
     9001 gtgacggcga tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg
     9061 aatcgcccag tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgcttgagc
     9121 tggatcttaa atcggttcgc ttgcagcaat ataaagagat ctttatttcc tgcgtgatga
     9181 tgctgttttg tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg
     9241 gtccgattcg caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc
     9301 gggtggaagg atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg
     9361 caatgtcgct ggctgcttat cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg
     9421 atctgcgtga aacgctggag cagatggaaa ttcagaacgt tgagttggat ctggcgaaaa
     9481 agcgcgccca ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc
     9541 tgcgtacacc actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa
     9601 caccaacgca gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa
     9661 ttattaatga tgttctcgac ttctcgaaac tggaagcagg taagctgatt ctggaaagta
     9721 ttccattccc actacgcagc acgctggatg aagtcgttac tctgctggca cattcttctc
     9781 acgataaagg gttggaactg acgctcaata ttaaaagcga cgtgcctgat aacgtgatcg
     9841 gcgacccact gcgattacag caaatcatca ctaacctggt ggggaatgca attaaattca
     9901 ccgagaatgg caacattgat attctggtag aaaaacgtgc gctgagtaat accaaagtgc
     9961 agattgaagt gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct
    10021 tattccaggc cttccgacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc
    10081 tggggctggt gattacacaa aaactggtta atgaaatggg cggcgatatt tcgttccata
    10141 gccagccgaa tcgcggttca actttctggt tccacattaa tctcgatctg aacccgaaca
    10201 ttattatcga agggccatcc atccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac
    10261 caaactccgc agcagcgcaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg
    10321 tttatagccc aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca
    10381 tcgcggtgac cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat
    10441 cgatgaccga tttcctgatg ctggcacttc cttgccatgc acaagttaat gctgaaaaac
    10501 tcaagcaaga tggtatcggc gcgtgtctgc tgaaaccatt aacacctacg cgcctgttgc
    10561 ctgccctgac ggaattttgt catcacaaac aaaacacgct tttgcctgta accgatgaaa
    10621 gtaagctggc aatgacagtc atggcggttg atgacaaccc cgctaacctg aaacttatcg
    10681 gcgcattgct ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcag
    10741 ttgaacgggc gaaacagatg ccgttcgatt tgatcttaat ggatattcaa atgccggaca
    10801 tggatggcat tcgggcctgc gaactcatcc accaactccc gcatcagcga caaacgccgg
    10861 ttatcgcggt aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga
    10921 tgagcgatta tctggcgaaa ccgattgaag aagagcgatt gcataatttg ttgttgcgct
    10981 acaagcctgg cagcggtatt tcgtctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg
    11041 tgaacccgaa tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg
    11101 atttagcgcg cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag
    11161 ttgaggaaca gctggctgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc
    11221 atggcagttg cggctatagc ggcgtaccac gtatgaagaa tctctgccag ctgatcgaac
    11281 aacagctacg tagcggtact aaagaagaag atttggagcc ggagctgctg gaactgttgg
    11341 acgagatgga taatgtcgcg cgcgaagcca gcaaaattct cgggtaacgg acgtcttgtc
    11401 gagtagcaat cggcttttta tgacgctggt ttcagattgg tgacaaagtg cctgtgttta
    11461 tgccggatgc ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta
    11521 aaaaatgatg tagacctgat acatagcaca acaggctatt ttgcgtttag catcagtctc
    11581 aaaccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat gcacgggcgc
    11641 ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatc gtcacgcgcg
    11701 ccaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat atcgatttct
    11761 acacccagcc ccggtttttc tggcacctgt accagcccgc ctttgatctc aaacggttct
    11821 ttggtcaggc gctgattacc ttcctgccag atccagtgcg tatcaatagc agtaatttta
    11881 cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgttt
    11941 gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct
    12001 tgcatagtcc agaaatgcgg atccgccagc gggatatcaa cggattgtaa cgagagcgta
    12061 tggcccatct gccgccagtc ggtggcgatc atattggttg cagtcggcag gccagtcgcg
    12121 cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct
    12181 tctgcataag ccagcgaacc tttcaggtat ttaccaattt taattgcttc gttcagcgac
    12241 caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca
    12301 atagactcgg cttcttcttc cccggccagt acaccgcctt tcagtttgaa atcgttgaag
    12361 ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct
    12421 tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagaggt
    12481 gtggctttgc gattaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt
    12541 tgaccatcgc ccagcagcga tgccacgttt acccccagat gctgccccag cagatccagc
    12601 attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa tgtctgcaaa
    12661 ccgcgcccac tagcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg
    12721 tattcaccca gcgttttacc taccaccagc ggaatcgcat cttccagcgt tttacggatt
    12781 ttctcgccgc cgggaatttc ccctacgcca gtgtgaccgg aattatcttt gataatcaca
    12841 atattacgcg tgaagaacgg tgcgtgtgca ccactcagat tcatcagcat actgtcatga
    12901 cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg agaactcata
    12961 tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg tcaaaggtcc
    13021 agccggggat caggtactgc atcggacctg cgtcgttacg cgcaccgcaa ggcagacgtt
    13081 tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc agaccgggcg
    13141 catcaggaac ggcaattttt ccgtttttaa tctccagcgg atttttggtc aggcgacaat
    13201 cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt gccgccgcgc
    13261 ccacatgggt aaatatcgcc agagagatat cgaaatggtt gttggaatgg cagccccagg
    13321 tcagccccca gtcgtcgcaa agctgcgcca cgcggactgc accggaaagc gtccagaagt
    13381 gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc
    13441 agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgtcga aactccgcca
    13501 tcacttcacg tccggagaag ccctgttctg cgccgcatgg atcttcggca taggtaagaa
    13561 catcattcag ccctttgcat aaagaaatgg cttcatcaag cagccatgca ccgttgggat
    13621 caacggtaat ccgcgcatcc gggaagcttt tcttcaatgc acgaacagtg tcgatttctt
    13681 gctcgccagg taacaccccg cccttaagtt tgaaatcttt aaagccatag cgatcctgtg
    13741 aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgg cgcaactgat
    13801 accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg gtccgatcac
    13861 ctatataaaa cagataaccg aggacggtaa tagtctcgcg ttgcttgcct ggccccaaca
    13921 gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaggcg gcttccagcg
    13981 ccgccacggc attaacgcgc aattcaaaag tccaggcacc tttgccaaag gtatcaaaat
    14041 cggctgcctg attaccttta tgtacctgct gaaccacttt attcaggcgc gcaacttcct
    14101 ggcccagaac catcggaata gcatcgacca gtgtttgata aatcacctct ccgcccggcg
    14161 cttcaccaat gccggtatgc ccggcgttat cggtgagtac cacgatattg cgggtgaagt
    14221 atgcgttatg cgcaccacca atattgagca acatgctgtc atgtccggcg accggaatga
    14281 ctttcatatc agtaataaca ggactggatt gtgtcgccat cttattgccc cgcgacaggt
    14341 ttcagctcga tacgcttgat atcgcccacc agcaccaggt agctcagtac cgcgattagg
    14401 gcatgaacac caacataaat cagcgcccca ttaaacgagc cagtcgtgcc aacgatataa
    14461 ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa caggccaccg
    14521 gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc cagcgccccg
    14581 atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc aacgttgacg
    14641 tagttgcaga acaccatcac catcgacagc aacatgccca ttacgatcgg tgttttacgc
    14701 gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataattcc acccagcaca
    14761 ccgccgataa aaccgcaaac cgccggaacg gaagccacaa agcccgcttt cagaatcgac
    14821 atcccacgcg cctgcaccag ataaaccgga aaccaggtaa tgaaaaagta agtcagggcg
    14881 ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag cagctgtttg
    14941 atctgccccc acttcacgct gaacggcact ttaactttgg tgctttgctg atccatattg
    15001 atcagcgcgc cacccgcggc gatatactcc agctctttct gatttacccc cggatgttga
    15061 tttggctcgt ggatgacttt caaccagata aagctgatga caatgcccag accgcccata
    15121 aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat cggcgcgaag
    15181 atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc cctttcctgc
    15241 accggaaacc aggccgcaac aatgcgactg ttgccgggga aagatggcgc ttcagcaagc
    15301 ccgaccagga agcgcagagt aaacagggca acgataatgc cgaatccact aaagatatcg
    15361 acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa gtagacgcgt
    15421 tttgaaccaa aacgatccag caaccaacca ccagggatct gcccgataac ataagcccat
    15481 gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc tttggccatt
    15541 tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt gacgataaac
    15601 aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct cgcagcctga
    15661 cttaaagaac tcattgttgc actcctgaaa attcgcgtta gccacgctca ctctggactg
    15721 cgacatcgcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt ataggtcgtt
    15781 cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc gccatagcgg
    15841 ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg gcattagccc
    15901 tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa gggtaaaaac
    15961 gaagaaataa agaagagtat gaaatggatc gcttgactct aggcaaacgc cagtaaaaat
    16021 ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa gattcgattc agcaccgcta
    16081 aaacgacatt taccgctcgc tgacatatca ggacaacagc gtgccccact gttcgaccca
    16141 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16201 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcaaaa
    16261 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16321 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16381 ggtcgtggac gtaaccacca gaacatactt atcctgatag ggcagccagt cgcttaattc
    16441 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16501 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16561 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16621 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16681 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16741 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16801 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    16861 cgtcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16921 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16981 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    17041 tccaccagtg gataatccaa caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    17101 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    17161 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17221 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17281 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17341 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17401 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17461 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17521 tcagccccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17581 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17641 cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacctcaat
    17701 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17761 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17821 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17881 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17941 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    18001 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    18061 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    18121 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18181 attaacaatc aacattt
//
LOCUS       DAZSIO010000075        17934 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZSIO010000075 DAZSIO010000000
VERSION     DAZSIO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.075"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(20..1018)
                     /gene="fbp"
                     /locus_tag="ACHX5T_002322"
     CDS             complement(20..1018)
                     /gene="fbp"
                     /locus_tag="ACHX5T_002322"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR8467500.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1194..2567
                     /gene="mpl"
                     /locus_tag="ACHX5T_002323"
     CDS             1194..2567
                     /gene="mpl"
                     /locus_tag="ACHX5T_002323"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR8467501.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2650..3201)
                     /gene="yjgA"
                     /locus_tag="ACHX5T_002324"
     CDS             complement(2650..3201)
                     /gene="yjgA"
                     /locus_tag="ACHX5T_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR8467502.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3295..4647
                     /gene="pmbA"
                     /locus_tag="ACHX5T_002325"
     CDS             3295..4647
                     /gene="pmbA"
                     /locus_tag="ACHX5T_002325"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR8467503.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4830..5212
                     /gene="cybC"
                     /locus_tag="ACHX5T_002326"
                     /pseudo
     CDS             4830..5212
                     /gene="cybC"
                     /locus_tag="ACHX5T_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5257..5721)
                     /gene="nrdG"
                     /locus_tag="ACHX5T_002327"
     CDS             complement(5257..5721)
                     /gene="nrdG"
                     /locus_tag="ACHX5T_002327"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR8467504.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5879..8017)
                     /gene="nrdD"
                     /locus_tag="ACHX5T_002328"
     CDS             complement(5879..8017)
                     /gene="nrdD"
                     /locus_tag="ACHX5T_002328"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR8467505.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8411..10066)
                     /gene="treC"
                     /locus_tag="ACHX5T_002329"
     CDS             complement(8411..10066)
                     /gene="treC"
                     /locus_tag="ACHX5T_002329"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR8467506.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10116..11537)
                     /gene="treB"
                     /locus_tag="ACHX5T_002330"
     CDS             complement(10116..11537)
                     /gene="treB"
                     /locus_tag="ACHX5T_002330"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR8467507.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11656..12603)
                     /gene="treR"
                     /locus_tag="ACHX5T_002331"
     CDS             complement(11656..12603)
                     /gene="treR"
                     /locus_tag="ACHX5T_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR8467508.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12788..12841
                     /gene="mgtL"
                     /locus_tag="ACHX5T_002332"
     CDS             12788..12841
                     /gene="mgtL"
                     /locus_tag="ACHX5T_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR8467509.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12982..15678
                     /gene="mgtA"
                     /locus_tag="ACHX5T_002333"
     CDS             12982..15678
                     /gene="mgtA"
                     /locus_tag="ACHX5T_002333"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR8467510.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFRSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15884..16270)
                     /gene="ridA"
                     /locus_tag="ACHX5T_002334"
     CDS             complement(15884..16270)
                     /gene="ridA"
                     /locus_tag="ACHX5T_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR8467511.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16343..16804)
                     /gene="pyrI"
                     /locus_tag="ACHX5T_002335"
     CDS             complement(16343..16804)
                     /gene="pyrI"
                     /locus_tag="ACHX5T_002335"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR8467512.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16817..17752)
                     /gene="pyrB"
                     /locus_tag="ACHX5T_002336"
     CDS             complement(16817..17752)
                     /gene="pyrB"
                     /locus_tag="ACHX5T_002336"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR8467513.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17756..17890)
                     /gene="pyrL"
                     /locus_tag="ACHX5T_002337"
     CDS             complement(17756..17890)
                     /gene="pyrL"
                     /locus_tag="ACHX5T_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR8467514.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
BASE COUNT         4192 a         4558 c         4817 g         4367 t
ORIGIN      
        1 tgtggagagg cgcaggagat tacgcgtccg ggaactcacg gataaagcgt tcgacatctt
       61 caaccatatg gtcgttgccg acaaagaacg aacggcgctg gtgcagagtt tccgggatga
      121 tatccagaat acgctctttg ccatcgctcg ctttaccgcc cgcttgttcc gccaggaatg
      181 ccatcgggtt gcactcataa agcaaacgca gtttgccgtc cgggtggctg gcggtgcttg
      241 ggtagagata aataccgcct ttcagcaggt tacggtggaa atccgcaacc agtgaaccga
      301 tataacgcga ggtatacggg cgattggtgg atttatcttc ttcctggcag aatttaatgt
      361 acttcttcac cccgttcgga aacttaatgt agtttccttc gttgatggag taggttttgc
      421 ctttctccgg gaagcgcatc cgttcctggc acaggcagaa aacgcccagc gaaggatcgt
      481 aagtaaaggc gtgaacaccg catccggtgg tgtaaaccag catggtagag gagccgtata
      541 ccacgtaacc tgccgcaacc tgtttgttac caggctggag gaaatcttct tccgttaccg
      601 gcgtgccaac aggcgtaacg cggcggtaga tggagaaaat ggtaccgaca gagacgttaa
      661 catcgatgtt ggacgagcca tccagggggt ccatcagcac cacgtatttt gcgtgttcac
      721 agccttcaaa gacgacaatc tcatcttctt cttcagaggc aatccccgca acgatatcgc
      781 gtgctttcag tgcggctttc agtttttcat tagcgaacaa gtcgagtttt tgctgaacct
      841 cgccctgcac gttctcagca ccgctggcac ccaggatatc aaccagtcct gctttgttga
      901 tatcgcgatg gataatcttg gcgcccagtt ttattgccga cagcaaagca gtgagctcac
      961 cggtagcatg agaaaactcg tgctgctttt cgacaataaa ttcacctaac gttttcataa
     1021 aactttccct gcaatgttta tggagtaaag cgaccgcaac aatcttaaca aataatctca
     1081 atgttgcgct caggtgaatc gcgccagcaa attgcggatt atcctgaaat gcgtttctca
     1141 cttgcccgac atatgcgtaa aatgagcggc agattaaaaa aggatagtga cgtatgcgca
     1201 ttcatatttt aggaatttgt ggcacattta tgggcggtct ggcgatgctg gcgcgccagt
     1261 taggccatga agtaacgggt tcggacgcca atgtgtatcc gccgatgagc accttacttg
     1321 agaagcaagg cattgagctg attcagggtt acgatgccag ccagctcgag ccgcagccgg
     1381 atctggtgat tattggcaac gccatgaccc gtggaaatcc gtgtgtggaa gcggtactgg
     1441 aaaaaaacat cccttatatg tcaggtccac agtggctgca cgattttgtg ctgcgcgacc
     1501 gctgggtgct ggccgttgcc ggtacacacg gcaaaaccac caccgcggga atggcgacct
     1561 ggattctgga acagtgtggt tacaaaccgg gatttgtgat cggcggtgtg ccggggaact
     1621 ttgaggtttc ggcgcgtctg ggcgaaagcg acttctttgt tatcgaagcg gatgagtacg
     1681 actgcgcctt cttcgacaaa cgctctaaat ttgtccatta ctgcccgcgt acgctgatcc
     1741 tcaacaacct tgagttcgat cacgccgata tctttgacga cctgaaagcg atccagaaac
     1801 agttccacca tctggtgcgt atcgttccgg ggcagggccg tattatctgg ccggaaaatg
     1861 acatcaacct gaaacagacc atggcgatgg gctgctggag cgagcaggag ctggtgggtg
     1921 agcagggtca ctggcaggcg aaaaagctga ccaccgatgc ttccgaatgg gaagttttgc
     1981 tggatggcga aaaagtgggc gaagtgaaat ggtcgctggt aggcgaacat aatatgcaca
     2041 atggcctgat ggcgattgca gcggctcgcc atgttggtgt agcgccggca gatgccgcta
     2101 acgcgctggg ttcgtttatt aatgctcgtc gccgtctgga gttgcgtggt gaagcgaatg
     2161 gcgtcacggt atatgacgat tttgcccatc acccgacggc gattctggca acgctggcgg
     2221 cgctgcgtgg caaagttggt ggtacggcgc gcattattgc tgtgctggaa ccgcgctcga
     2281 ataccatgaa aatggggatc tgcaaagacg atctggcacc ttcattaggt cgtgctgatg
     2341 aagtcttcct gctgcaaccg gcgcatattc cgtggcaggt ggcagaagtg gcagaagcct
     2401 gcgttcagcc tgcacactgg agtggcgatg tggatacgct ggcagatatg gtggtgaaaa
     2461 ccgctcagcc tggcgaccat attctggtga tgagcaacgg cggttttggt gggatccatc
     2521 agaaactgct ggatggtctg gcgaagaagg cggaagctgc gcagtaattc ggcttcagcc
     2581 tgatatagca ttgccggata aggcgtttac gccgcatccg gcaaaccata ctcacatcaa
     2641 caacgaaaat tacccttcat tctccgccag ttcgcgcaga tactggaaaa tctggcgtgc
     2701 ggatttcggc ggcttattcc cttctttctc tttcttcgcg ttgcggatca gtgtacgcag
     2761 ttgctgacga tcggcatcgg gccacagatt caatacttca gcgatggcgt catcaccttg
     2821 atcgatcaga cgatcgcgca ggttttccag tttatggaac agcaccacct gctggttgtg
     2881 acggtttttc agcttatcca gcgcctgacg aataggctct acgtcgcgct ggcgcagcat
     2941 cttaccgatg agctgcaact ggcggcggcg accttccatc ttaatacgct gggcgagctc
     3001 aatagccggg cgcagatccg catctaacgg gatcttatcc agcgcgtttt tccccagatc
     3061 aacaatttcc gcgccaaggc gttttagctc ctcggcatca cgtttaattt cacttttact
     3121 gacccagata atttcatcgt cttcgtcttc gatgtcatca ccgggaacgt cgtcgagcca
     3181 gtcttcgggc tgcttagtca tctcaggctc cttaaaaaaa gaggctaatg ttaccagtta
     3241 agatgcgcac tgaaaaacgg ttctctgtta gacttcagag aaactctcta cattatggca
     3301 cttgcaatga aagtaatctc tcaagttgaa gcgcagcgca agattctgga agaagcagtt
     3361 tccactgcgc tggagttggc ctcaggcaaa tcggacggtg cggaagttgc cgtcagcaag
     3421 accaccggca ttagcgtaag cacgcgttat ggtgaagtgg agaatgttga attcaatagt
     3481 gatggcgcgc tggggatcac tgtttatcac cagaaccgta aaggtagcgc atcgtctacc
     3541 gatttaagcc cgcaggccat tgcccgcacc gtacaggcgg cgctggatat tgcccgttat
     3601 acctcgccag atccctgtgc cggcgtggca gacaaagagc tgctggcctt tgacgcaccg
     3661 aatctcgact tgttccaccc tgcggaagtt tccccggatg aagccattga actggcggcc
     3721 cgcgcagaac aggcggcatt gcaggcggac aaacgcatca ccaataccga aggtggcagc
     3781 tttaacagcc actacggtgt caaagttttt ggcaacagcc acggcatgtt gcagggttac
     3841 tgctcaacgc gtcattcgct ctccagctgt gtaattgccg aagaaaatgg cgatatggag
     3901 cgtgattacg cctacaccat tggccgcgcg atgagcgatc tgcaaacgcc agagtgggtc
     3961 ggggccgact gtgctcgccg tactttatcg cgcctgtcac cgcgtaaact ctccaccatg
     4021 aaagcgccgg tcatttttgc caatgaagtg gcaaccgggc tttttggcca tctggtgggg
     4081 gcgatagcgg gtggctcggt ttaccgtaaa tctacctttc tgctggattc gctgggtaaa
     4141 caaattctgc cggactggct gaccattgaa gagcatccgc atctgctgaa agggctggcg
     4201 tcgacgccat tcgacagcga aggtgtgcgc accgagcgtc gcgatattat taaagatggc
     4261 atcctgactc agtggctgct gaccagctac tcggcgcgga aactggggct gaaaagcacc
     4321 ggacatgcgg gcggtattca caactggcgg attgccggac aagggctaag cttcgaacaa
     4381 atgctcaaag agatgggcac cgggctggtg gtgacggagc tgatgggcca gggcgtgagt
     4441 gccattaccg gtgattattc ccgtggtgca gcgggcttct gggtagagaa tggcgaaatt
     4501 cagtatcccg ttagtgaaat caccatcgca ggtaatttaa aagatatgtg gcgcaatatt
     4561 gtcaccgtcg gtaacgatat tgaaacacgc agtaatatac agtgtggttc tgtgctgttg
     4621 ccggagatga aaatcgccgg gcagtaaaaa actggcgcga cctgccgcgc tataaactaa
     4681 aaaattacac aaaatcattc gcactgcatc gagacggaaa gtgaatgagg gccggggagc
     4741 gtactcgcag tacgtgaccc gggcgaatga gcgtagccgt cgatgaggca gtgtgaagga
     4801 tgaagtgtaa ataaaaaagg aagtgagcaa tgcgtaaaag cctgttagct attcttgcag
     4861 tctcctcgtt ggtattcagt tctgcgtcgt ttgccgctga tcttgaagac aatatggaaa
     4921 ccctcaacga caatttaaaa gtgatcgaaa aagcggataa cgcggcgcaa gtcaaagacg
     4981 cgttaacgaa gatgcgcgcc gcagcgctgg atgcgcaaaa agcaacgccg ccgaagctcg
     5041 aagataaatc accggacagc ccggaaatga aagatttccg ccacggtttc gacattctgg
     5101 tcggtcagat tgacgacgcg ctgaagctgg caaatgaagg taaaaagaag cgcaggctgc
     5161 tgcagagcaa ctgaaaacga cccgcaacgc ctatcaccag aagtatcgtt aattcctcat
     5221 ttccctgttg cctgcactca ggtaacaggg aacttatcat cgcaaatgat gcaccacctg
     5281 attgctgctg ccgcgccaga tcagggaagg gtcttttaaa tcctgcacaa atttgccgtc
     5341 gaccagcacg ttaatcaaat caacaacctg catttgcgca gcgttgagtt cgtcgagttt
     5401 atagcctgtc cacacccaga tgtctttacc cggacactcg gcgcggatgc gttttaccag
     5461 tttcagaata tccggcacgt tttgcggatg cagcggatcg ccgccggaga gggaaatccc
     5521 ctggcgtttg atacgagtgt cattcagatc tttaatgatc ttgtcttcca ttgctttggt
     5581 aaatggctga ccagaattta cccgccatgt gcttttgtta tagcaaccgg ggcattcatg
     5641 aacacacccg gagacaaaca gggtgcagcg agtgccgggg ccgttgacga tgtcgacagg
     5701 atagtactga tgataattca ttatttcgcc tggcttatca gtccgcgtgt aggctgcatc
     5761 cggtaatcaa tgcctgatgc gacgctgtcg cgtcttatca ggcctacagc cgttgccaaa
     5821 tgtaggccgg ataaggcgtt tacgccgcat ccggcatttg cttagcgcag agtgaagatt
     5881 aacctatctg cccattcccc aaatgtttaa cgcggcgctt aacttcttcc tgcttaccag
     5941 cgttaaacgg acgtgcatcc gggctaccta aatatccgca cacgcggcga gttaccgaca
     6001 cacgggaggc gtcatggtta ccacatttcg ggcaagtgaa gcctttgctg gtgcactcga
     6061 actcaccggt aaagccacac tcgtagcact catcaatcgg tgtattggtg ccgtaatacg
     6121 gtacatgctg atagctgtaa tcccagacat cttccagcgc tttcaggttg tgctgaatgt
     6181 ttggatactc gccgtagcaa atgaaaccac cgttcgccag cggcgggtaa ggcgcttcaa
     6241 agtcgatctt gtcgtacggg ttcaccttct tctccacatc aaggtggaaa ctgttggtgt
     6301 agtaaccttt atcggtcacg cccggcacca cgccaaactc agcagtatcg agacggcaga
     6361 agcggtcgca caggttttca ctcggcgtgc tgtaaagact gaaaccataa cccgtttctt
     6421 ctttccactg atccactgcc tgacgcagac gttcaacaat ggcgatacct ttcgcgcgaa
     6481 gcttctcgtt gtcgtaaaca tgctcgccgc tgaacagcgc gttaatggtt tcgtggatgc
     6541 cgatgtaacc cagcgaaata gacgcacgac cgtttttgaa gatttcagaa acatcatcgt
     6601 cagcattcag acgtacgcca caagcacctt ccatatagag gatcggggcg acgcgcgctt
     6661 tcacgccttc gagacgagcg atacgggtca tcagtgcctt acgtgccagc accagacgtt
     6721 catccagcag cttccagaag gtggcttcat cgccttttgc ttccagagca atacgcggca
     6781 ggttcaggct gatcacgccg aggttgttac gaccatcgtg gatctgctcg ccgttttcat
     6841 tttcccacac gccgaggaag ctgcggcagc ccatcggggt tttaaacgaa ccggtgacgt
     6901 tcactacctg atcgtagttc aggatatccg gatacatgcg cttgcttgcg cactccagcg
     6961 ccagctgttt gatgtcgtag ttcggatcgc cttttttatg gttcaggcca tcgcgaatcg
     7021 caaacaccag tttcgggaac accgcagttt tacggttttt accgaggcct gcgatacggt
     7081 tacgcaggat cgattcctga atcaggcgcg attcccagct ggtgcccaga ccaaaaccaa
     7141 aggttacaaa cggcgtctga ccgttggcgg tgtgcagggt gtttacttcg tactccagcg
     7201 actggaaggc atcgtagcac tctttgatgg tacgagagtt agcgtagcct tcggcgtccg
     7261 ggatgttcca ctcttctgcg gtcttgcgat gtttgttgta gctggcggta acaaacggcg
     7321 ccagcacttc atcgatacgg ttaatggtgg tgccgccata aatatggctg gcgacctgag
     7381 caataatctg cgcagttacc gcggttgccg tagagatcga cttcggcggt tcaatctcgg
     7441 cgttccccat tttaaaaccc tgggtcagca tgcctttcag gtcgatcaac atgcagttga
     7501 acatcgggaa gaacggcgag taatcgagat cgtgatagtg aatatcgcca cgctcatgtg
     7561 cctgcaccac gtcacgcggc agcaggtgct ggcgtgcata gtgtttagcc acgatcccgg
     7621 ccagcaggtc gcgctgggtt ggaatcacct tgctgtcttt gttggcgttt tcgttgagta
     7681 acgaggcgtt ggtctgctcg accagaccac ggatctcctg gttcaggcga ccgcgttttt
     7741 cacgttcaat gtcgcgatcg tgacggtact cgatgtaagc acgagccagt tgtttgtatg
     7801 gacccgacat cagctggttt tcaactgcgg tctggatctc gttgatatcc acctggttgc
     7861 gcccctgcat ctgctcgttg acaaccgcgg caacagtggc gcaataatcg gcatcatcga
     7921 cttccgctgc tttagctgca cgcagaatcg cttctttgat gcgctctgat ttaaacggca
     7981 ctttgcagcc gtctcgtttc atcacatgcg gtgtcatgat cgctccatat ttttaagaac
     8041 aggttatcca cagaaattgg gaaaggcgtt ttccggtttt ttcgtttcgt tttccggtgc
     8101 tttccgcaat cgccatccgc ttatccacaa cgccgcacct ttttcgtggg gagctgtagt
     8161 agcaattata gtcgattaat acaacatatt gggttgggac gcattttaaa gtctatatat
     8221 agtgctttgc atcaaggatg ttttaacttt ttttgatgta gctcaaagta aaaagcagag
     8281 cgtacggatg acgggcgcta cagcgttatg taaatttttt aatgaatttg ctggttgaaa
     8341 aatcaacaaa aacaacatgc tgacagacaa aaccccggaa tgaccggcat tatcggggct
     8401 tagggaagat ttacttctgt aaccaccaga cagcctcaaa aggccgtaaa ttcatggcac
     8461 agggttgtgg tgaggcttct tcgtagttat gcatcacaag ctgccagttg ccgcgcattt
     8521 gccctggctg ccagggttgg atctcacggc taaggttggc aatgaccagc aaggtttgcc
     8581 ccttccattc acggcgatag caccacaata cagggctgtt tggcagcaga tcctggtaat
     8641 tgccccatgt caggatggct tcttgcttac gcagtgcgat taacttttgg taggtgtaaa
     8701 acacagagga gtcatcggcc agcgcggctt ctacgttgat ttgttgatag ttatcgccta
     8761 ggccaatcca cggttcgcca gccgtaaacc cggcattatc gccgttgctc cattgcatgg
     8821 gcgtgcggct gttgtcacgg gatttactgg cgaggattgc caataactcg tcggcatcac
     8881 gaccatcgtt gcgcagctcg gcaaacatat tgaggctctc cacgtcgcga tagtcagtaa
     8941 tgcgcgtgaa atgcgggttg gtcatgccaa tctcttcgcc ctggtagata tacggcgttc
     9001 cctgcatgcc atgcagcacc atcgccagca tttttgccgc aggcacgcgg tattcacctt
     9061 catcaccaaa gcgagaaaca atgcgcggct gatcgtggtt acaccagaac aaggcattcc
     9121 atgccacgtt gtgcattcct tgttgccagt ggcggaacaa tgttttcaac gccacaaagt
     9181 caggtttagc cagcgtccat ttttcaccac cgggataatc gaccttcagg tgatgaaaat
     9241 taaaggtcat cgacaattca ctgcctgtca gtgccgcgta tcgctggcaa tgctcaaggc
     9301 tggtggagga catttcacct acggtcatta acccgcgtgg cgtaaacaca tcgcggttca
     9361 tctcgtgcaa aaactcgtgt gctcgtggcc cgtcggtgta gaagcgacgc ccgtcgccat
     9421 ccaggtcttc agggaaacgc gggtctttgg agatcagatt caccacatcc aggcgcaacc
     9481 cgtcgacccc acgatcggcc cagaattcac agactttttt cagctctgcg cgtaccgctg
     9541 gattctccca gttgagatcc gcctgttctg gtgcaaagag atgcaaatag tactgttcgc
     9601 tttccgcatg ccagcgccac gcactaccgc caaattttga acgccagttg ttcggtggcg
     9661 tttctggttc gccatcgcgc cagatataaa actggcggta agggctttct ttgttcagcg
     9721 cctcgcgaaa ccaggcatgt tgggtagagg tatggttaaa caccatatcg agaatgatac
     9781 gaatcccgcg cgattttgcc tgcgtcacca gttcgtcaaa atcgtccagc gtgccgtagg
     9841 tgggatcaat cgccgtatag ttcgctacat cgtaaccgtt atcgacctgg ggagagacat
     9901 aaaagggggt tagccagatg gcatcaacgc ccagtttatg cagatagtcc aggcgttgga
     9961 taacgccacg taaatcgccg gtgccgctac ccgtggtgtc ctgaaaactc tttggataaa
    10021 tctggtagat aacgccgttt tgccaccagt ggggaagatt agtcattacg ttattcctgc
    10081 aaatgcgagg gggcgcaatt gcgccccgaa gaaaattaaa caatgtccag cgtgcccagg
    10141 cggtatttcc gctgatagat aaacgaggtg agtacaatcg ggatgatgat ggcgatagcc
    10201 attgccagcg caaatacctg ccagtagctc ggttgaatag agagaattcc cggcaggccc
    10261 cctacgccga taccattcgc cataacgccg ttcaggccgc atagcaatcc tgccagacca
    10321 gaaccaatca tcgcgcacag catcgggaag cgatatttca ggttgatgcc gtacattgca
    10381 ggctcagtga ccccaagcca ggcggagata gcggcaggca cggagatctc gcgttcattg
    10441 tgcttgcggc tggaaatgat aatgcctatc acggcggagc cctgagcgat attcgacagc
    10501 gcaatcagcg gccacactgg cgtgccaccc atgctttgaa tcatctgcaa atcaatagca
    10561 agggtggtct ggtgtacgcc ggtgatcacc agcggcgcgt acaggaagcc aaacaatgcc
    10621 gcaccaatcg gagcaaagct gccggtcatc aggtgacgta ccgcaaaggc aacgccatcg
    10681 ccaatcatgc gaccaaacgg accaatcagc gcatgggcga ggaacaccgc gaggatcagc
    10741 gaacagacgg gcaccaccac cagatagagg taatccggta cgatgcgttt aaggcgagtt
    10801 tcaataacgc ccagcgccag cccggctaac agtgccggga tcacctgcgc ctgatagccc
    10861 actttggcga tgctgaacat gccaaagtcc cacacttccg gcagctgctg cccgagcaga
    10921 taagcgttca tcagctgggg ggaaaccagt gtcacgccaa gcacgatacc aaggatcggc
    10981 gtgccgccca tttttttcac cgctgaccag caaatcccga ccggcaggta gaagaaaatc
    11041 gcttcaccga tcaaccacag aaaatcgtag atcgtttgca gggaagggta catttgcgcc
    11101 agcgtctgac cgttgctcat gggcaaatcg ccgatcacat tgcgaaaacc gaggatcaaa
    11161 ccgccgctaa tcaacgcggg cagcaacggg aagaagatct ccgcgaaatg agagatcaac
    11221 tgctcatgcc atttcatatt ttgccgggcg gcttttttta cctgctcttt atcaacctgc
    11281 gcctgtccgg ttgacgctat tagtgcttga tagtaatcac ccacgttggt gccaatcacc
    11341 acctgaaatt gcccggcatt ggtgaaacag cctttcacca tggggagttg ctcaatttct
    11401 ttcggtctgg cattggccgg ttggttgagg acaaagcgca ggcgagtaat acagtggctc
    11461 accgtcgcaa tattgccgcg cccgccgacc agttcaatca accgatcgat atccgtttgg
    11521 tttattttgc tcatcataaa gccccatggc agatgacatt tttggttggc tgcagaatat
    11581 attgcgcgga aaaatttaaa aacgggaacg ttcccgaaac gcagcgaaga tcacaattta
    11641 tcgttcagga aacgatcagg acagggtggc ggggatgatg atttgttgcg gttcgctgcg
    11701 cccggttacc tgtgcgatca attggcaggc cgcctggcgt ccagcttcgg cgtagccagg
    11761 atctacggtg acgatctccg gatgaaggaa tttcattaac ggcgtattac ctacgctcgc
    11821 cagttgcaag gtgtcgatgc gttgctcttg caggtattta cttgcgccaa gagccagcgt
    11881 gtcggttgcg cacagtaacg cggtagtttc aggcgtaatc acttttgcta cgttttcata
    11941 gccttgcttc atagcaagcc ctggcagagc ggcaacggga tgcagtttat gcgctttgca
    12001 gaacgccagg taggcttcgt gacgtcgctt cccggtcgtc acgtcactat gcggcacgcc
    12061 gagataactg atattgcgat gcccctggtc atagagccgt tgcatcagaa tcttgattgc
    12121 cccttcgtcg tcataacaga ccgaagcaaa gccttttgcg tcacgcgcca gcagaaccag
    12181 cgatgactgc cagtgggcta acatttcttc ggttatgccg gtaaaaccga acagcactac
    12241 gccgtcgata ttacgccgtt tcagcacccc caaatgttcg gcaactaatt gtggggaaaa
    12301 ctgattttcc atcatgattg gatcgtaacc ttgttcatag aacgctggca gcatggtttg
    12361 aacggcgaga ttttctgaca acgaatccag acgggtaaca atgatggcga ccactttatc
    12421 gctttgccca cgcatagcgc gcgcagagcg ggaaggggaa aatccatgct gattcatcac
    12481 tgcttcaaca cgctcgcgcg tgcgctgact cacgccgctt tcgttattca gcacccggga
    12541 aaccgtagat ttccccacgc cgcttaagcg cgcgatgtct ttgatggtca gccgattttg
    12601 catcctgttg tcccataacg tgttgtttaa ttatttgagc ctaactttac ccgtgcattc
    12661 agcaatgggt aaagtctggt ttatcgttgg tttagttgcc agcaggtatt atatcgccat
    12721 aaatgctacg aatattattg gattctcctt attatttgcg gcgctttttt cacttaccgg
    12781 aggttatatg gaacctgatc ccacgcctct ccctcgacgg agattaaaac ttttccggta
    12841 agcccgtctt ttcacggcgt taccggatgc gtaaggccgt gacgttttaa tgtccctgct
    12901 cagctttatt accttcaggt aaggcttcgc cacgcctgaa gacatttctg tactgtttca
    12961 gacagtgcgg agggactcct tatgtttaaa gaaattttta cccggttcat tcgccattta
    13021 ccttcccgtc tggttcatcg cgatccattg cctggcgcgc agcagacagt gaatacggcg
    13081 gtcccgccgt ccttaagtgc gcattgcctg aaaatggcgg tgatgcccga agaagaattg
    13141 tggaaaacgt tcgacaccca tccggaagga ttaaatcagg cggaagtgga atctgcccgc
    13201 gaacaacatg gtgataataa attacccgcc caacaaccgt cgccgtggtg ggtacattta
    13261 tgggtctgct atcgcaaccc ctttaatatt ttgctcacca ttcttggtgc tatttcttac
    13321 gccacggaag atttatttgc cgcaggtgtt atcgcgctaa tggtcgctat ttctacgttg
    13381 ctgaacttta ttcaggaagc tcgttccacc aaagcggcag atgccctgaa agcgatggtc
    13441 agtaataccg cgacggtact gcgagtaatt aacgacaaag gcgaaaatgg ctggctggag
    13501 atcccgatag accagctggt gcccggcgat attataaaac tggcggcggg ggatatgatc
    13561 ccggcagatt tacgtatctt gcaggcgcgg gatctgttcg tcgctcaggc gtcgttaacc
    13621 ggtgagtctc tgcccgtgga aaaagccgct actactcgcc agccggagca cagcaatccg
    13681 ctggagtgcg acaccctgtg ttttatgggc accaccgtgg tgagcggcac ggcacaagca
    13741 atggtgattg ctacaggagc caacacctgg tttggtcaac tggcggggcg tgttagtgag
    13801 caggaaagcg agccgaatgc ctttcagcaa gggatcagcc gcgttagtat gctgctgatt
    13861 cgctttatgc tggtgatggc tccggtggtg ttgcttatca acggttacac caaaggcgac
    13921 tggtgggaag cggcactgtt tgcgctttcg gtcgcggtag gcttaacgcc ggaaatgttg
    13981 ccgatgattg ttacctcgac gctggcgcgc ggcgcagtaa agctgtcgaa acagaaagtg
    14041 atcgtcaaac atctggatgc cattcagaac tttggcgcaa tggatattct gtgcactgat
    14101 aaaaccggca ccctgacgca ggacaaaatt gtgctggaga atcataccga tatctccggt
    14161 aaaaccagcg aacgcgtgct gcatagcgcg tggttgaaca gtcattacca gaccggactt
    14221 aaaaatctgc ttgataccgc ggtgctcgaa ggtacggatg aagagtcagc gcgttcgctg
    14281 gccagccgtt ggcaaaaaat tgatgagatt ccgtttgatt tcgagcgtcg tcggatgtcg
    14341 gtggtagtgg cagaaaatac cgaacaccat cagctggttt gcaaaggtgc attgcaggaa
    14401 atcctcaatg tgtgttcgca ggtgcgtcac aatggcgaga ttgtaccgct cgatgacacc
    14461 atgctgcgta agattaagcg ggttactgat acgctgaatc gtcaggggct gcgcgtggtt
    14521 gcggtggcga cgaaatacct gccagcgcgt gaaggagatt accagcgggc ggatgaatcc
    14581 gacctgatcc tcgaaggata tattgctttc cttgatccgc caaaagagac aaccgctccg
    14641 gcactgaagg cattaaaagc gagtggaatt accgtaaaaa tccttactgg cgacagtgag
    14701 ttagttgcag cgaaagtgtg ccatgaagtg gggctggatg cgggagaggt ggtcattggt
    14761 agtgatattg aaacgctatc tgacgatgaa ctggcaaatc tcgcacagcg caccacgctg
    14821 tttgcccgcc tgacgccgat gcataaagaa cgcattgtga ctttactgaa gcgcgaaggg
    14881 catgtggttg gctttatggg cgatggtatt aatgatgcgc ccgccttacg cgctgcggat
    14941 atcggcattt ctgtggacgg cgcggtagat attgcccgtg aagcggctga tatcatcctg
    15001 ctggaaaaaa gcctgatggt gctggaagag ggggttattg agggacgtcg cactttcgcc
    15061 aacatgctga aatacatcaa attgacggcg agttctaact tcggtaatgt gttcagcgtg
    15121 ctggtggcga gtgctttctt gcccttcctg ccgatgttgc cgttacactt gcttattcag
    15181 aacctgctgt acgatgtgtc acaggtggcg atcccgtttg ataacgtcga cgacgagcaa
    15241 attcaaaagc cgcagcgttg gaatccggcg gatctggggc gctttatggt cttcttcgga
    15301 ccgatcagct cgattttcga tattctgacg ttttgcttga tgtggtgggt attccatgcc
    15361 aacacgccgg aaacgcaaac gctgttccgg tcgggctggt ttgtggtggg attactgtcg
    15421 caaacgctga ttgtgcatat gatccgtacc cgccgtgtgc cgtttattca gagctgtgca
    15481 tcgtggccgt taatgatcat gaccgtgatc gtgatgattg tcgggatcgc attgccgttt
    15541 tcaccgctgg ccagttatct gcagctgcag gcgctgccgt taagctattt cccgtggctg
    15601 gttgcgattc tggcagggta tatgacgtta acccagttgg tgaaagggtt ctatagccgt
    15661 cgttacggct ggcagtaaag aataaactgg gcatggtggc ccggattcag attgcagaca
    15721 aagtgagctt tgttcatgcc ggatgcggcg taaacgccca atccggccta caaattcaat
    15781 aaattgcagg aatcacgtag gcctgataag cgtagcgcat cgggcgattt tgcttttgtc
    15841 atcagactta atccgggcat gatagcccgg atttccatca agattagcga cgaacagcga
    15901 ttgcttcgat ctcaatcttc acgtctttcg gcagacgggc aacttcaacg caagaacgtg
    15961 ccgggaaagt ggcgttgtgt tcggtgaaga aggcttcgta agtggcgttt acggttgcga
    16021 agtcgttcag atctttcacg aacacagtgg ttttaacgat gtcgcccact ttcaggccag
    16081 cggcttcgac gattgctttt acattatcca gcgactgacg tgcctgtgca gcgacgtctg
    16141 ccggtacttc accagttttc ggatttaccg ggatctgacc ggaggtgatg atcatattgc
    16201 ccagatcaac gccctgtacg taaggaccga tagctgccgg tgcattttcc gtcgcgatag
    16261 ttttgctcat gatttctcct ttattacagc ggtaaaaagt ctggctcatt atagggagcc
    16321 agacttttat taccaaccgc aattaattgg ccagcaccac attatgggaa aactcttttt
    16381 cacagtattt gcatttaagc gcgatgtcat tggcgcgttt tcgcacggca aagctggatg
    16441 aaaccggttc ggcatggctg atacagttgc tgttcgggca gaccagcaca ttgtcgatgc
    16501 gctctggcag acttgggcgc gatttaccca ccacttcata gttgtcgata cggttaaccg
    16561 tggcttgcgg tgcatacaat gccagttggt ctacttgctc ttcactcaaa aaggtatttt
    16621 cgattttgat cagatctttg cggcccatct cgccagaagg caaattcaga ccaatggtga
    16681 tgcgctgatc tgtttcggtc aacttgaaca gactcaacag cttaaaaccg atctgggcgg
    16741 ggatatggtc aattaccgtg ccgcgtttaa tagcttcaac ctgcaattta ttatcgtgtg
    16801 tcatctctat ttccccttac agtaccagat cgcgattcag aaccagtgcc agtaacgcct
    16861 ggcgagcgaa aatcccgttg cctgcttgct ggaagtacca ggcgtgaggc gttttatcca
    16921 catccgtcgc aatctcatca acacgcggca gcggatgcag cactttcata ttggctttgg
    16981 cgttgtggag atcgctggcg cgaagaacaa actgcgcttt cacgttggcg tactcggacg
    17041 ggtccagacg ctctttttgc acgcgggtca tgtacaggat gtctacttcc gccatcactt
    17101 cttcaataga gctgtgcaga ctccaggcga tccctttttc atcgagcata tccagaatgt
    17161 attgcggcat tgccagcgca tccggcgcga taaagtaaaa acggttgccg tcgaacttcg
    17221 ctagcgcctg ggtcagggag tgaacggtgc ggccatattt caggtcacca accattgcga
    17281 cgtggagatt gtccagacgc ccctgggttt cctgaatggt gaataagtcc agcaaggttt
    17341 gcgtcggatg ttggttggag ccatcaccgg cattcagtac cggtacattg ccagaaaatt
    17401 cggtggctag gcgtgccgca ccttcctgcg gatgacgcat cactatcgca tcgacgtaag
    17461 tgctgataac cgaaatggta tcggccagcg tttcgccttt tttacccagt gatgtattgg
    17521 cgctgtcgga gaagcccacc acgctggcac ccaggcggtg catggaagtt tcgaaagaga
    17581 ggcgggtacg ggtagaggct tcgaagaaac agctggcaat gactttgtgc ttcaacagct
    17641 ctggttgcgg gtttgctttc agtttcgccg ctgtcgccag caccagatta aggtcatcgc
    17701 gactaaggtc gtttatggaa atgatatgtt tctgatatag cggattagcc atcttttatc
    17761 tcctgacacc taggcaaaaa aaagcccctc gattgagggg ctgggaatgg gtgatcaacg
    17821 ggaagaaaaa cggcaggcca gcgtcttttt tcagacgcgg taagacaaaa tgtcgaacac
    17881 actgaaccat acatcctccc ggcaaattgt ccggcattat actcatagcc agaa
//
LOCUS       DAZSIO010000076        17814 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZSIO010000076 DAZSIO010000000
VERSION     DAZSIO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17814
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.076"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(120..1403)
                     /gene="gltA"
                     /locus_tag="ACHX5T_002338"
     CDS             complement(120..1403)
                     /gene="gltA"
                     /locus_tag="ACHX5T_002338"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR8467515.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1609..1716)
                     /locus_tag="ACHX5T_002339"
     CDS             complement(1609..1716)
                     /locus_tag="ACHX5T_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8467516.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2097..2501
                     /gene="sdhC"
                     /locus_tag="ACHX5T_002340"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2097..2501
                     /gene="sdhC"
                     /locus_tag="ACHX5T_002340"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR8467517.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2495..2842
                     /gene="sdhD"
                     /locus_tag="ACHX5T_002341"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2495..2842
                     /gene="sdhD"
                     /locus_tag="ACHX5T_002341"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR8467518.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2842..4608
                     /gene="sdhA"
                     /locus_tag="ACHX5T_002342"
     CDS             2842..4608
                     /gene="sdhA"
                     /locus_tag="ACHX5T_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR8467519.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4624..5340
                     /gene="sdhB"
                     /locus_tag="ACHX5T_002343"
     CDS             4624..5340
                     /gene="sdhB"
                     /locus_tag="ACHX5T_002343"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR8467520.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5641..8442
                     /gene="sucA"
                     /locus_tag="ACHX5T_002344"
     CDS             5641..8442
                     /gene="sucA"
                     /locus_tag="ACHX5T_002344"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR8467521.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8457..9674
                     /gene="odhB"
                     /locus_tag="ACHX5T_002345"
     CDS             8457..9674
                     /gene="odhB"
                     /locus_tag="ACHX5T_002345"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR8467522.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            9768..10934
                     /gene="sucC"
                     /locus_tag="ACHX5T_002346"
     CDS             9768..10934
                     /gene="sucC"
                     /locus_tag="ACHX5T_002346"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR8467523.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10934..11803
                     /gene="sucD"
                     /locus_tag="ACHX5T_002347"
     CDS             10934..11803
                     /gene="sucD"
                     /locus_tag="ACHX5T_002347"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR8467524.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11906..12628)
                     /gene="mngR"
                     /locus_tag="ACHX5T_002348"
     CDS             complement(11906..12628)
                     /gene="mngR"
                     /locus_tag="ACHX5T_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR8467525.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12798..14714
                     /gene="mngA"
                     /locus_tag="ACHX5T_002349"
     CDS             12798..14714
                     /gene="mngA"
                     /locus_tag="ACHX5T_002349"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR8467526.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14732..17367
                     /gene="mngB"
                     /locus_tag="ACHX5T_002350"
                     /pseudo
     CDS             14732..17367
                     /gene="mngB"
                     /locus_tag="ACHX5T_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
BASE COUNT         4345 a         4424 c         4809 g         4236 t
ORIGIN      
        1 agaatatatg aatgtccgat agtggatttt atttttacaa ttttttgtgt ttaggcatat
       61 aaaaattaac ccgccatttg aacggcgggt taaaatattt acaacttagc aatcaaccat
      121 taacgcttga tatcgctttt aaagtcgcgt ttttcatatc ctgtatacag ctgacgcgga
      181 cgggcaatct tcataccgta actgtgcatt tcgctccagt gggcgatcca gccaacggta
      241 cgtgccattg cgaaaatgac ggtgaacatg gaagacggaa tacccatcgc tttcaggatg
      301 ataccagagt agaaatcgac gttcgggtac agtttcttct cgataaagta cgggtcgttc
      361 agcgcgatgt tttccagctc catagccact tccagcaggt catctttggt gcccagctct
      421 ttcagaactt catggcaggt ttcacgcatt acggtggcgc gcgggtcgta atttttgtac
      481 acgcggtgac cgaagcccat caggcggaaa gaatcatttt tatctttcgc acgacgaaca
      541 aattccggaa tgtgtttaac ggagctaatt tcttccagca ttttcagcgc cgcttcgtta
      601 gcaccaccgt gcgcaggtcc ccacagtgaa gcaatacctg ctgcgataca ggcaaacggg
      661 ttcgcccccg aagagccagc ggtacgcacg gtggaggtag aggcgttctg ttcatggtca
      721 gcgtgcagga tcagaatacg gtccatagca cgttccagaa tcggattaac ttcatacggt
      781 tcgcacggcg tggagaacat catattcagg aagttaccgg cataggagag atcgttgcgc
      841 ggataaacaa atggctgacc aatggaatac ttgtaacaca tcgcggccat ggtcggcatt
      901 ttcgacagca ggcggaacgc ggcaatttca cgatgacgag gattgttaac atccagcgag
      961 tcgtgataga acgccgccag cgcgccggta ataccacaca tgactgccat tggatgtgag
     1021 tcgcgacgga aagcgtggaa cagacgggta atctgctcgt ggatcatggt atgacgggtc
     1081 accgtagttt taaattcgtc atactgttcc tgagtcggtt tttcaccatt cagcaggatg
     1141 taacaaactt ctaggtagtt agaatcggtc gccagctgat cgatcgggaa accgcggtgc
     1201 agcaaaatac cttcatcacc atcaataaaa gtaattttag attcgcagga tgcggttgaa
     1261 gtgaagcctg ggtcaaaggt gaacacacct tttgaaccga gagtacggat atcaataaca
     1321 tcttgaccca gcgtgccttt cagcacatcc agttcaacag ctgtgtcccc gttgaggttg
     1381 agttttgctt ttgtatcagc catttaaggt ctccttagcg ccttattgcg taagactgcc
     1441 ggaacttaaa tttgccttcg cacatcaacc tggctttacc cgtttgttat ttggctcgcc
     1501 gctctgtgaa agaggggaaa acctgggtac agagctctgg gcgcttgcag gtaaagaatc
     1561 cattgatgac gaataaatgg cgaatcaagt acttagcaat ccgaattatt aaacttgtct
     1621 accactaata actgtcccga atgaattggt caatacttcc acactgttac ataagttaat
     1681 cttaggtgaa ataccgactt cataactttt acgcattata tgcttttcct ggtaatgttt
     1741 gtaacaactt tgttgaatga ttgtcaaatt agatgattaa aaattaaata aatgttgtta
     1801 tcgtgacctg gatcactgtt caggataaaa cccgacaaac tatatgtagg ttaattgtaa
     1861 tgattttgtg aacagcctat actgccgcca ggtctccgga acaccctgca atcccgagcc
     1921 acccagcgtt gtaacgtgtc gttttcgcat ctggaagcag tgttttgcat gacgcgcagt
     1981 tatggaaagg acgctgtctg acccgcaagc agaccggagg aaggaaatcc cgacgtctcc
     2041 aggtaacaga aagttaacct ctgtgcccgt agtccccagg gaataataag aacagcatgt
     2101 gggcgttatt catgataaga aatgtgaaaa aacaaagacc tgttaatctg gacctacaga
     2161 ccatccggtt ccccatcacg gcgatagcgt ccattctcca tcgcgtttcc ggtgtgatca
     2221 cctttgttgc agtgggcatc ctgcagtggc ttctgggtac cagcctctct tcccctgaag
     2281 gtttcgagca agcttccgcg attatgggca gcttcttcgt caaatttatc atgtggggca
     2341 tccttaccgc tctggcgtat cacgtcgtcg taggtattcg ccacatgatg atggattttg
     2401 gctatctgga agaaacattc gaagcgggta aacgctccgc caaaatctcc tttgttatta
     2461 ctgtcgtgct ttcacttctc gcaggagtcc tcgtatggta agcaacgcct ccgcattagg
     2521 acgcaatggc gtacatgatt tcatcctcgt tcgcgctacc gctatcgtcc tgacgctcta
     2581 catcatttat atggtcggtt ttttcgccac cagtggcgaa ctgacatatg aagtctggat
     2641 cggtttcttc gcctctgcgt tcaccaaagt gttcaccctg ctggcgctgt tttctatctt
     2701 gattcatgcc tggatcggca tgtggcaggt gttgaccgac tacgttaaac cgctggcctt
     2761 gcgcctgatg ctgcaactgg tgattgtcgt tgcactggtg gtttacgtga tttatggatt
     2821 cgttgtggtg tggggtgtgt gatgaaattg ccagtcagag aatttgatgc agttgtgatt
     2881 ggtgccggtg gcgcaggtat gcgcgcggcg ctgcaaattt cccagagcgg ccagacctgt
     2941 gccctgctct ctaaagtctt cccgacccgt tcccataccg tttctgcgca aggcggcatt
     3001 accgttgcgc tgggtaatac ccatgaagat aactgggaat ggcatatgta cgacaccgta
     3061 aaagggtcgg actatatcgg tgaccaggac gcgattgaat atatgtgtaa aaccgggccg
     3121 gaagcgattc tggaactcga acacatgggc ctgccgttct cgcgtctcga tgatggtcgt
     3181 atctatcaac gtccgtttgg cggtcagtcg aaaaacttcg gcggcgagca ggcggcacgc
     3241 actgcggcgg ctgctgaccg taccggtcac gcactgttgc acacgcttta tcagcagaac
     3301 ctgaaaaacc acaccaccat tttctccgag tggtatgcgc tggatctggt gaaaaaccag
     3361 gatggcgcgg tggtgggttg taccgcactg tgcatcgaaa ccggtgaagt ggtttatttc
     3421 aaagcccgcg ctaccgtgct ggcgactggc ggagcagggc gtatttatca gtccaccact
     3481 aacgcccaca ttaacactgg cgacggtgtc ggcatggcta tccgcgcagg cgtaccggtg
     3541 caggatatgg aaatgtggca attccacccg accggcattg ccggtgcggg cgtactggtc
     3601 accgaaggtt gccgtggtga aggcggttat ctgctgaaca aacatggcga acgctttatg
     3661 gagcgttatg cgccgaacgc caaagacctg gcgggccgtg atgtggtggc gcgttccatc
     3721 atgattgaaa tccgtgaagg tcgcggctgt gatggtccgt gggggccaca cgcgaaactg
     3781 aaactcgatc acctgggtaa agaagttctc gaatcccgtc tgccgggtat cctggagctt
     3841 tcccgtacct tcgctcacgt cgatccggtg aaagagccga ttccggttat cccaacctgt
     3901 cactacatga tgggcggtat tccgaccaaa gttaccggtc aggcgctgac tgtgaatgag
     3961 aaaggcgaag atgtggttgt tccgggactg tttgccgttg gtgaaatcgc ttgtgtatcg
     4021 gtacacggcg ctaaccgtct gggcggcaac tcgctgctgg acctggtggt ctttggtcgc
     4081 gcggcaggtc tgcatctgca agagtctatc gccgagcagg gcgcactgca cgatgccagc
     4141 gagtctgatg ttgaagcgtc tctggatcgc ctgaaccggt ggaacaataa tcgtaacggt
     4201 gaagatccgg tggcgatccg taaagcgctg caagaatgta tgcagcataa cttctcggtc
     4261 ttccgtgaag gtgatgcgat ggcgaaaggg cttgagcagt tgaaagtgat ccgcgagcgt
     4321 ctgaaaaatg cccgtctgga tgacacttcc agcgagttca acacccagcg cgttgagtgc
     4381 ctggaactgg ataacctgat ggaaacggcg tatgcaacgg ctgtttctgc caacttccgt
     4441 accgaaagcc gtggcgcaca tagccgcttc gacttcccgg atcgtgatga tgaaaactgg
     4501 ctgtgccact ccctgtatct gcctgagtcg gaatccatga cgcgccgaag cgtcaacatg
     4561 gaaccgaaac tgcgcccggc attcccgccg aagattcgta cttactaatg cggagacagg
     4621 aaaatgagac tcgagttttc aatttatcgc tataacccgg atgttgatga tgctccgcgt
     4681 atgcaggatt acaccctgga agcggaagaa ggtcgcgaca tgatgctgct ggatgcgctt
     4741 atccagctga aagagaaaga tcccagcctg tcgttccgcc gctcctgccg tgaaggtgtg
     4801 tgcggttccg acggtctgaa catgaacggc aagaatggtc tggcctgtat taccccgatt
     4861 tcggcactca accagccggg caagaagatt gtgattcgcc cgctgccagg tttaccggtg
     4921 atccgcgatt tggtggtaga catgggacaa ttctatgcgc aatatgagaa aattaagcct
     4981 tacctgttga ataatggaca aaatccgcca gctcgcgagc atttacagat gccagagcag
     5041 cgcgaaaaac tcgacgggct gtatgaatgt attctctgcg catgttgttc aacctcttgt
     5101 ccgtctttct ggtggaatcc cgataagttt atcggcccgg caggcttgtt agcggcatat
     5161 cgtttcctga tcgatagccg tgataccgag actgacagcc gcctcgacgg tttgagcgat
     5221 gcattcagcg tattccgctg tcacagcatc atgaactgcg tcagtgtatg tccgaagggg
     5281 ctgaacccga cgcgcgccat cggccatatc aagtcgatgt tgttgcaacg taatgcgtaa
     5341 actgtaggcc tgataagacg cgcaagcgtt gcatcaggca accagtgccg gatgcggcgt
     5401 gaacgcctta tccggcctac aagtcattac ccgcaggcct gataagcgca gcgcatcagg
     5461 cgtaacaaag aaatgcagga aatctttaaa aactgcccct gacactaaga cagtttttaa
     5521 aggttccttc gcgagccact acgtagacaa gagctcgcaa gtgaaccccg gcacgcacat
     5581 cactgtgcgt ggtagtatcc acggcgaagt aagcataaaa aagatgctta agggatcacg
     5641 atgcagaaca gcgctttgaa agcctggttg gactcttctt acctctctgg cgcaaaccag
     5701 agctggatag aacagctcta tgaagacttc ttaaccgatc ctgactcggt tgacgctaac
     5761 tggcgttcga cgttccagca gttacctggt acgggagtca aaccggatca attccactct
     5821 caaacgcgtg aatatttccg ccgcctggcg aaagacgctt cacgttactc ttcaacgatc
     5881 tccgaccctg acaccaatgt gaagcaggtt aaagtcctgc agctcattaa cgcataccgc
     5941 ttccgtggtc accagcatgc gaatctagat ccgctgggac tgtggcagca agataaagtg
     6001 gccgatctgg atccgtcttt ccacgatctg accgaagcag acttccagga aaccttcaac
     6061 gtcggttcat ttgccagcgg caaagaaacc atgaaactcg gcgagcttat ttccgccctc
     6121 aagcaaacct actgcggccc gattggtgcc gagtatatgc acatcaccag caccgaagaa
     6181 aaacgctgga tccaacagcg tatcgagtct ggtcgcgcga ctttcaatag cgaagagaaa
     6241 aaacgcttct taagcgaact gaccgccgct gaaggccttg agcgttacct cggcgcaaaa
     6301 ttccctggcg caaaacgctt ctcgctggaa ggcggtgacg cgttaatccc gatgcttaaa
     6361 gagatgatcc gccacgctgg caacagcggc acccgcgaag tggttctcgg gatggcgcac
     6421 cgtggtcgtc tgaacgtgct ggtgaacgtg ctgggtaaaa aaccgcaaga cttgttcgac
     6481 gagttcgccg gtaaacataa agaacacctc ggcacaggcg acgtgaaata ccacatgggc
     6541 ttctcgtctg acttccagac cgatggcggc ctggtgcacc tggcgctggc gtttaacccg
     6601 tctcaccttg agattgtaag cccggtcgtt atcggctctg ttcgtgcccg tctggacaga
     6661 cttgatgagc cgaacagcaa caaagtgctg ccaatcacca tccacggtga cgccgcagtg
     6721 accgggcagg gcgtggttca ggaaactctg aacatgtcga aagcgcgtgg ttatgaagtt
     6781 ggcggtacgg tacgtatcgt tatcaacaac caggttggtt tcaccacctc taacccgctg
     6841 gatgcccgtt ctacaccgta ctgtactgat atcggtaaga tggttcaggc accgattttc
     6901 cacgttaacg cggatgatcc ggaagccgtt gcctttgtga cccgtctggc gctcgatttc
     6961 cgtaacacct ttaaacgtga tgtcttcatc gacctggtat gctaccgccg tcacggccac
     7021 aacgaagccg acgagccgag cgcaacccag ccactgatgt atcagaaaat caaaaaacat
     7081 ccgacaccgc gcaaaatcta cgctgacaag ctggagcagg aaaaagtggc gacgctggaa
     7141 gatgccaccg agatggttaa cctgtaccgc gatgcgctgg atgctggcga ttgcgtagtg
     7201 gcagagtggc gtccgatgaa catgcactct ttcacctggt cgccgtacct caaccacgaa
     7261 tgggacgaag agtacccgaa caaagttgag atgaagcgcc tgcaggaact ggctaaacgt
     7321 atcagtacgg taccagaagc agttgaaatg cagtctcgcg ttgccaagat ttatggcgat
     7381 cgccaggcga tggcagccgg tgagaaactg ttcgactggg gcggtgcgga aaacctcgct
     7441 tacgccacgc tggttgatga aggcattccg gttcgcctgt cgggtgaaga ctccggtcgc
     7501 ggtaccttct tccaccgcca cgcggtgatc cacaaccagt ctaacggttc cacttacacg
     7561 ccgctgcaac acatccataa cggtcagggc gcgttccgtg tctgggactc cgtactgtca
     7621 gaagaagccg tgctggcgtt tgaatatggt tatgccaccg cagaaccacg caccctgacc
     7681 atctgggaag cgcagttcgg tgacttcgcc aacggtgcgc aggtggttat cgaccagttc
     7741 atctcctctg gcgagcagaa atggggccgg atgtgtggtc tggtgatgtt gctgccgcac
     7801 ggttacgaag ggcaggggcc ggagcactcc tccgcgcgtc tggaacgtta tctgcaactt
     7861 tgtgctgagc aaaacatgca ggtttgcgta ccgtctaccc cggcacaggt ttaccacatg
     7921 ctgcgtcgtc aggcgctgcg cgggatgcgt cgtccgctgg tcgtgatgtc gccgaaatcc
     7981 ctgctgcgtc atccgctggc ggtttccagc ctcgaagaac tggcgaacgg caccttcctg
     8041 ccagccatcg gtgaaatcga cgagcttgat ccgaagggcg tgaagcgcgt agtgatgtgt
     8101 tctggtaagg tttattacga cctgctggaa cagcgtcgta agaacaatca acacgatgtc
     8161 gccattgtgc gtatcgagca actctacccg ttcccgcata aagcgatgca ggaagtgttg
     8221 cagcagtttg ctcacgtcaa ggattttgtc tggtgccagg aagagccgct caaccagggc
     8281 gcatggtact gcagccagca tcatttccgt gaagtgattc cgtttggggc ttctctgcgt
     8341 tatgcaggcc gcccggcctc cgcctctccg gcggtagggt atatgtccgt tcaccagaaa
     8401 cagcaacaag atctggttaa tgacgcgctg aacgtcgaat aaataaagga tacacaatga
     8461 gtagcgtaga tattctggtc cctgacctgc ctgaatccgt agccgatgcc accgtcgcaa
     8521 cctggcataa aaaacccggc gacgcagtcg tacgtgatga agtgctggta gaaatcgaaa
     8581 ctgacaaagt ggtactggaa gtaccggcat cagcagacgg cattctggat gcggttctgg
     8641 aagatgaagg tacaacggta acgtctcgtc agatccttgg tcgcctgcgt gaaggcaaca
     8701 gcgccggtaa agaaaccagc gccaaatctg aagagaaagc gtccactccg gcgcaacgcc
     8761 agcaggcgtc tctggaagag caaaacaacg atgcgttaag cccggcgatc cgtcgcctgc
     8821 tggctgaaca caatctcgac gccagcgcca ttaaaggcac cggtgtgggt ggtcgtctga
     8881 ctcgtgaaga tgtggaaaaa catctggcga aagccccggc gaaagagtct gctccggcag
     8941 cggctgctcc ggcggcgcaa ccggctctgg ctgcacgtag tgaaaaacgt gtcccgatga
     9001 ctcgcctgcg taagcgtgtg gcagagcgtc tgctggaagc gaaaaactcc accgccatgc
     9061 tgaccacgtt caacgaagtc aacatgaagc cgattatgga tctgcgtaag cagtacggtg
     9121 aagcgtttga aaaacgccac ggcatccgtc tgggctttat gtccttctac gtgaaagcgg
     9181 tggttgaagc cctgaaacgt tacccggaag tgaacgcgtc tatcgacggc gatgacgtgg
     9241 tttaccacaa ctatttcgac gtcagcatgg cggtttctac gccgcgcggc ctggtgacgc
     9301 cggttctgcg tgatgtcgat accctcggca tggcagacat cgagaagaaa atcaaagagc
     9361 tggcagtcaa aggccgtgat ggcaagctga cggttgaaga tctgaccggt ggtaacttca
     9421 ccatcaccaa cggtggtgtg ttcggttccc tgatgtctac gccgatcatc aacccgccgc
     9481 agagcgcaat tctgggtatg cacgctatca aagatcgtcc gatggcggtg aatggtcagg
     9541 ttgagatcct gccgatgatg tacctggcgc tgtcctacga tcaccgtctg atcgatggtc
     9601 gcgaatccgt gggcttcctg gtaacgatca aagagttact ggaagagccg acgcgtctgc
     9661 tgctggacgt gtagtagttt aagcttcacc tgcactgtag accgacgtgg atagggtgtc
     9721 ggtctacggt ttaaaagata acgattactg aaggatggac agaacacatg aacttacatg
     9781 aatatcaggc aaaacaactg tttgcccgct atggcttacc agcaccggtg ggttatgcct
     9841 gtactactcc gcgcgaagca gaagaagccg cttcaaaaat cggtgccggt ccgtgggtag
     9901 tgaaatgtca ggttcacgct ggtggccgcg gtaaagcggg cggtgtgaaa gttgtaaaca
     9961 gcaaagaaga catccgtgct tttgcagaaa actggctggg caagcgtctg gtaacgtatc
    10021 aaacagatgc caatggccaa ccggttaacc agattctggt tgaagcggcg accgatatcg
    10081 ctaaagagct gtatctcggt gccgttgtag accgtagttc ccgtcgcgtg gtctttatgg
    10141 cctccaccga aggcggcgtg gaaatcgaaa aagtggcgga agaaactccg cacctgatcc
    10201 ataaagttgc gcttgatccg ctgactggcc cgatgccgta tcagggacgc gagctggcgt
    10261 tcaaactggg tctggaaggt aaactggttc agcagttcac caaaatcttc atgggcctgg
    10321 cgaccatttt cctggagcgc gacctggcgc tgatcgaaat caacccgctg gtcatcacca
    10381 aacagggcga tctgatttgc ctcgacggca aactgggcgc tgatggcaac gccctgttcc
    10441 gccagcctga tctgcgcgaa atgcgtgacc agtcgcagga agatccgcgt gaagcacagg
    10501 ctgcacagtg ggaactgaac tacgttgcgc tggacggtaa catcggttgt atggttaacg
    10561 gcgcaggtct ggcgatgggt acgatggaca tagttaaact gcacggcggc gaaccggcta
    10621 acttcctgga cgttggcggc ggcgcaacca aagaacgtgt aaccgaagcg ttcaaaatca
    10681 tcctctctga cgacaaagtg aaagccgttc tggttaacat cttcggcggt atcgttcgtt
    10741 gcgacctgat cgctgacggt atcatcggcg cggtagcgga agtgggtgtt aacgtaccgg
    10801 tcgtggtacg tctggaaggt aacaacgccg aactcggcgc gaagaaactg gctgacagcg
    10861 gcctgaatat tattgcagca aaaggtctga cggatgcagc tcagcaggtt gttgccgcag
    10921 tggaggggaa ataatgtcca ttttaattga taaaaacacc aaggttatct gccagggctt
    10981 taccggtagc caggggactt tccactcaga acaggccatt gcatacggca ctaaaatggt
    11041 tggtggcgta actccaggta aaggcggcac tacccacctc ggcctgccgg tgttcaacac
    11101 cgtgcgtgaa gctgttgctg ctactggcgc ttccgcgtct gttatttacg taccagcacc
    11161 gttctgcaaa gactccattc tggaagctat cgatgcaggc atcaaactga ttatcaccat
    11221 cactgaaggc atcccgacgc tggatatgct gaccgtgaaa gtgaaactgg acgaagcagg
    11281 cgttcgtatg atcggcccga actgcccagg cgttatcacc ccgggtgaat gcaaaattgg
    11341 tatccagccg ggtcacattc acaaaccggg taaagtgggt atcgtttccc gttccggtac
    11401 actgacctat gaagcggtta aacagaccac ggattacggt ttcggtcagt cgacctgtgt
    11461 cggtatcggc ggtgacccga tccctggctc taactttatc gacattctcg aaatgttcga
    11521 aaaagatccg cagaccgaag cgatcgtgat gatcggtgag atcggcggta gcgctgaaga
    11581 agaagcggca gcgtacatca aagagcatgt taccaagcca gttgtgggtt acatcgctgg
    11641 tgtgactgcg ccgaaaggca aacgtatggg ccacgcgggt gcgatcattg ccggtgggaa
    11701 agggactgcg gatgagaaat tcgctgctct ggaagccgca ggcgtgaaaa ccgttcgcag
    11761 cctggcggat atcggtgaag cactgaaaac tgttctgaaa taaatatctg taataagaaa
    11821 tagccctcgc cgcttccctc tacaggaatg gcgaagggct gtcggtttcg acatggttgg
    11881 ccatcgtatg atggcctttt tgtgcttatc gcgatgattt tcgctgcgct atcagggtaa
    11941 atttatagtc atcggtatta aaagcgttgc ggctatattc aaacacccga ccatcaacta
    12001 aatatccacg cgatactttt tcaagaatcg gctttgtctg gctgatatta agcagacggc
    12061 tcatctcttc ggttggcatc agaggaatga tttcctgttc gctacgatcg ataaccatct
    12121 tcttcacttc ttcaataaag tgatatttcg aattttccat gacctgccag gtgagatccg
    12181 ggaacaacgc aagcggcatc caggtttctt ccagcgccat tggcttttgc ttgcgatagc
    12241 gcacgcgctt cacatgccaa acacgatcct gcacggtgat ttgtagctgt tgctgaagaa
    12301 aatcgtcagc cggaatcact tcaaatatca gaacttcact gtgtgtatcg acgtgacggt
    12361 ccgacagttt ttcatcaaaa ctggttaact gaaaaatatc gtaattgacc cgctcttctt
    12421 tgacgtaagt cccgctgccc tgaatgcttt cgaggatctg ttgctcgact aactggcgca
    12481 aagcctgacg caccgtaacc cggctgacgc caaactctgt ttgtaatgcc gattcggtag
    12541 gtaacgcatc gccaggttta agctcgccac gcgcaatttg ctcacgaatg cgatcagcaa
    12601 tctgtcggta taagggtttg tgtcccatta tttagcatct cattaatacg aatttagcca
    12661 ttatgcccga taaactcatc ctgtaaacaa tacaaataaa atgcaaataa tttcaatcaa
    12721 gtgaaattga ttacgcaatg gtattgtttt gtcgggcaca ctggcgcgac tataaaaacg
    12781 atcaagtgag gatcatgatg aacctgacga ctctgaccca ccgcgatgcg ttgtgtctga
    12841 atgcgcgctt taccagccgt gaagaggcca ttcacgcgtt gactcaacgt cttgctgctc
    12901 tggggaaaat ttccagtact gagcaatttc tgaaagaagt gtatcgccgt gaaagccttg
    12961 gcccgaccgc cttaagtgaa gggttggctg tgccgcatgg caaaactgct gcggtaaaag
    13021 aagcggcgtt tgcagtcgcc acactcagcg agccgcttca gtgggaaggc gtggatggcc
    13081 cggaagcggt tgatttagtg gtgttgctgg cgattcccct caatgaagcg ggtactacgc
    13141 acatgcaact gctgacagcg ctgaccacgc gcctggcgga tgatgagatt cgggcgcgta
    13201 tacagtcggc gacgacgcct gatgaattgc tctcggcgct ggatgacaag ggaggcacgc
    13261 aaccttctgc ctctttttcc aacgcgccaa ctatcgtttg cgtaacggcc tgcccggcgg
    13321 gtattgctca cacctacatg gctgcggagt atctggaaaa agccggacgc aaactcggcg
    13381 tgaatgtcta cgttgaaaag caaggtgcta acggtattga agggcgttta acggcggatc
    13441 aactcaatag tgcaactgcc tgtatttttg cggctgaagt cgccatcaag gagagtgagc
    13501 gttttaacgg cattcctgcg ctttctgtgc ctgttgccga gccgattcgc catgcagaag
    13561 cgttgatcca acaatcgctt accctcgagc gtggcgatga gacgcgtacc ttacagcaag
    13621 atacgcaacc ggtgaaaagc gtcaaaacgg agctgaaaca ggcactgttg agcgggatct
    13681 cttttgccgt accgttgatt gtcgcggggg ggacggtgct ggcggtcgcg gtattactgt
    13741 cgcaaatctt cgggctacaa gatctgttta atgaagaaaa ctcctggctg tggatgtacc
    13801 gcaaactggg cggcgggctg ctcggaattt tgatggtacc ggtgctggca gcctataccg
    13861 cctattcgct ggcagataaa ccggcgttag cgccaggctt tgcggctgga cttgccgcca
    13921 acatgatcgg ctccgggttt ctcggcgcgg tcgttggcgg attgatagcc ggttacttga
    13981 tacgctggat gaaaaatcac ttgcgtctta gcagtaaatt caatggcttc ctgacttttt
    14041 atctctaccc ggtgctcggt acattgggag cgggcagtct gatgctgttt gtggtggggg
    14101 aacctgtcgc ctggatcaat aactcgctta ccgcctggct gaacggtctg tcaggaagta
    14161 acgcgctgtt gctgggtgcc attctcggtt ttatgtgttc ctttgacctt ggagggccag
    14221 tgaataaagc cgcttatgca ttctgcctgg gcgcaatggc gaacggcgtt tacggcccgt
    14281 atgccatttt cgcctccgtc aaaatggttt cggcatttac cgtaaccgct tccacgatgc
    14341 tcgcaccgcg cctgtttaaa gagtttgaaa ttgagaccgg gaaatccacc tggctgttag
    14401 ggctggcagg tattaccgaa ggggcgatcc cgatggcgat tgaagatccg ctgcgggtta
    14461 ttggttcgtt tgtgctgggc tctatggtaa cgggcgctat tgtcggtgcg atgaatatcg
    14521 gcctttcgac acccggtgct ggcattttct cgctcttttt acttcatgat aatggcgcgg
    14581 gcggtgttat ggcggcaatt ggctggtttg gtgcggcatt ggtgggggct gcaatctcga
    14641 ctgcaattct cctgatctgg cggcgtcacg cggttaagca tggcaactat ctgactgatg
    14701 gcgtaatgcc ataaacaaaa aggaaacgac gatgaaagcg gtatctcgcg ttcacatcac
    14761 cccgcatatg cactgggatc gggagtggta tttcaccacc gaagagtcac gcattctgct
    14821 ggtcaataat atggaagaga tcctgtgccg actggaacag gacaacgaat acaaatatta
    14881 cgtactcgac gggcaaacgg cgatcctcga agattatttc gcggtgaaac cggaaaacaa
    14941 agaccgcttg aagaaactgg tagaagccgg caagttgatt atcggcccct ggtataccca
    15001 gaccgatacc acgattgttt ctgcggaatc catcgtccgt aatctgatgt acggaatgcg
    15061 tgactgcctc gcgtttggcg agccgatgaa aataggttat ttacccgatt cctttggcat
    15121 gtccgggcaa ctgccgcata tctacaatgg atttggcatt acctgcacca tgttctggcg
    15181 tggatgttct gagcgccacg gtacggataa aaccgagttt ttgtggcaaa gcagtgacgg
    15241 tagcgaagtg acggcgcagg tgatgccgtt gggttatgcc atcggtaagt acttacctgc
    15301 ggacgaaaac ggattacgca aacgcctcga cagttatttt gacgtgctgg aaaaagcgtc
    15361 tgtaaccaaa gagattttgc tgccgaatgg gcatgatcag atgccattgc agcaaaatat
    15421 cttcgaagtg atggagaaac tgcgcgaaat ttatcctcaa cgaaagtttg tgatgagccg
    15481 ctttgaagag gtatttgaga agatcgaagc gcagcgagat aatctggcaa ccctgaaagg
    15541 ggaatttatt gatggcaaat atatgcgcgt gcatcgcacc atcggttcca cgcgcatgga
    15601 tatcaaagtt gcccacgcac gtattgaaaa taagattgtt aatctgctgg aaccgctggc
    15661 aacgctggcc aggacgttgg gttttggata ccaccacggc ttgctggaga aaatgtggaa
    15721 agagatctta aaaaatcatg cccacgacag catcggctgc tgctgtcgtg acaaagttca
    15781 tcgcgaaatc gtcgcccgct tcgaactggc tgaagacatg gcggataatc tgctgcgttt
    15841 ctatatgcgt aaaatcgccg acaacatgcc gcagagcgac gccgacaaac tcgtcctgtt
    15901 taacctgatg ccctggccgc gggaagaagt gatcaacacc actgtgcggc tgcgcgccag
    15961 ccagtttaat ctacgagacg atcgcggtca gcctgtaccg tattttattc gccatgcccg
    16021 tgagatcgat ccaggcctaa tcgatcggca aatagttcat tacggtaatt acgatccctt
    16081 tatggagttt gatatacaga tcaaccagat tgtcccttct atgggctatc gcacgcttta
    16141 tatcgaagcg aatcagcctg gcaacgtgat tgcggcaaaa agtgacgctg aagggatact
    16201 ggaaaatgct ttctggcaaa ttgcgctcaa tgaggatgga acgctacaac tggtagataa
    16261 agatagcggt gtgcgctatg accgggtgtt gcaaattgaa gaaagctctg atgatggtga
    16321 tgaatatgac tattcacccg caaaagaaga gtgggtaatt acttcggcga atgcgaaacc
    16381 gcaatgcgag attattcatg aggcctggca gagcagggct gttatccgct atgaaatggc
    16441 ggtgccgcgt aatctgcaac aacgcagcgc aagacaatcc actggcagag tagatgtcga
    16501 gttggttgtc actcttagtc ataacaacag gcgcattgat gtggatatca accttgataa
    16561 ccaggctgac gatcatcgcc ttcgcgtcct gatccctaca tcttttaaca ccgatagtgt
    16621 tctggcagat acccagtttg gttcgctaac gcgccccgtg aacgacagtg caatgaacaa
    16681 ctggcagcaa gaaagctgga aagaagcgcc ggttccggta tggaatatgc tcaactatgc
    16741 agccttacag gaagggcgta acggcatggc tgtctttagc gaagggctac gtgaatttga
    16801 agtcatcggt gaagagaaga aaacctttgc cattacgttg ctgcgcggcg tgggcttact
    16861 ggggaaagaa gatctgcttt taaggcctag gcggccttcg ggaattaaaa tgccagtccc
    16921 ggactcacaa ctacgtggtc tgctttcttg tcgcctaagt ttattgagtt ataccggtac
    16981 gccaaccgcc gctggtgtag ctcagcaggc gcgagcatgg ctgactccag tacagtgtta
    17041 caacaaaatc ccatgggatg cgatgaagct caacaaagcc ggattcaacg tgccggaaag
    17101 ttatagtttg ttgaaaatgc ccccagtggg atgcccctga taagcgcact taagaaagct
    17161 gaagaccgac aagaagtgat tttacggctg tttaatccgg ctgaatcagc aacctgtgat
    17221 gcgactgttg ctttcagtcg cgaggtgatt tcttgctcag aaacgatgat ggatgaacac
    17281 attaccaccg aggaaaatca aggttcaaat ctatcggggc cttttttacc cggccagtca
    17341 cggacgttca gttaccggct tgcctgaata gcaatcaaac cgaagccaca tatgcgcggc
    17401 cagattgttg acaaagggcg ctttgttcat gccggatacg gcatgaacgc tttattcggt
    17461 ctacaaaagc aggcaaattc aatatattgc agagatgatg tagacactgg caagcgtagc
    17521 gcatcaggta attttgtgtt tatcttcact ctcaagctac gtatatgtgg ctttattttt
    17581 aacaaaataa taaccacggg tgagttaatt ataatataat tataagttaa ttaaatgtta
    17641 atatcggcgg gagggagatt tatgccttta ttaataatcc tgaaactctg cgtcgtatta
    17701 gccagtgacc aaaaaaagaa ttaaggtcaa ccgtgctgtt tttgcttcgt ctctttttat
    17761 ctttaattgc caaccgaaac taatttcagt cttataattc acacatttta aaca
//
LOCUS       DAZSIO010000077        17780 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZSIO010000077 DAZSIO010000000
VERSION     DAZSIO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.077"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            176..859
                     /locus_tag="ACHX5T_002351"
     CDS             176..859
                     /locus_tag="ACHX5T_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR8467527.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            903..5565
                     /locus_tag="ACHX5T_002352"
                     /pseudo
     CDS             903..5565
                     /locus_tag="ACHX5T_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            5933..7588
                     /gene="lldP"
                     /locus_tag="ACHX5T_002353"
     CDS             5933..7588
                     /gene="lldP"
                     /locus_tag="ACHX5T_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR8467528.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7588..8364
                     /gene="lldR"
                     /locus_tag="ACHX5T_002354"
     CDS             7588..8364
                     /gene="lldR"
                     /locus_tag="ACHX5T_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR8467529.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8361..9551
                     /gene="lldD"
                     /locus_tag="ACHX5T_002355"
     CDS             8361..9551
                     /gene="lldD"
                     /locus_tag="ACHX5T_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR8467530.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9747..10220
                     /gene="trmL"
                     /locus_tag="ACHX5T_002356"
     CDS             9747..10220
                     /gene="trmL"
                     /locus_tag="ACHX5T_002356"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR8467531.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10273..11094)
                     /gene="cysE"
                     /locus_tag="ACHX5T_002357"
     CDS             complement(10273..11094)
                     /gene="cysE"
                     /locus_tag="ACHX5T_002357"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR8467532.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGCGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11174..12193)
                     /gene="gpsA"
                     /locus_tag="ACHX5T_002358"
     CDS             complement(11174..12193)
                     /gene="gpsA"
                     /locus_tag="ACHX5T_002358"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR8467533.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12193..12660)
                     /gene="secB"
                     /locus_tag="ACHX5T_002359"
     CDS             complement(12193..12660)
                     /gene="secB"
                     /locus_tag="ACHX5T_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR8467534.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12723..12974)
                     /gene="grxC"
                     /locus_tag="ACHX5T_002360"
     CDS             complement(12723..12974)
                     /gene="grxC"
                     /locus_tag="ACHX5T_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR8467535.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13116..13547)
                     /locus_tag="ACHX5T_002361"
     CDS             complement(13116..13547)
                     /locus_tag="ACHX5T_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR8467536.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13792..15336
                     /gene="gpmM"
                     /locus_tag="ACHX5T_002362"
                     /gene_synonym="pgmI"
     CDS             13792..15336
                     /gene="gpmM"
                     /locus_tag="ACHX5T_002362"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR8467537.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15370..16629
                     /gene="envC"
                     /locus_tag="ACHX5T_002363"
     CDS             15370..16629
                     /gene="envC"
                     /locus_tag="ACHX5T_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR8467538.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16633..17592
                     /locus_tag="ACHX5T_002364"
     CDS             16633..17592
                     /locus_tag="ACHX5T_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFR8467539.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17579..17780)
                     /locus_tag="ACHX5T_002365"
                     /pseudo
     CDS             complement(17579..17780)
                     /locus_tag="ACHX5T_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4452 a         4620 c         4648 g         4060 t
ORIGIN      
        1 catacaaaca cagacatctt ttgaaacgaa tgatacattt ttatgatgaa tgcttaaagc
       61 aatatttaat cgacacacat cactgtcatt aatgtaaagc aacatagctc accaataata
      121 caatggacaa ctctaccggc attgagcgct ggtcggagga aataaggatt tttacatgaa
      181 cttgaagaag atttttttca gtgctgtcac tgtttcagtt ctctgcgcat taacgggatg
      241 tgactacatt gaagaaggca agccagaaag tagtttactg aaacaacaag aagaacataa
      301 taataaaatt gtgctacttg aaaaacaaca agcacagcta aagagtcaac tcgaaactat
      361 tcaaaaacaa caaactggca taataaacag tacaaagact ttaactcacg taattaaatc
      421 cgttaaagac caacaaaata cttttatttt taccgagttt aacccagcaa aaacgaagta
      481 tttcatcctg aacaacggtt cggtggcttt agcgggtcga gtgttatcca ttgacgccac
      541 agagaatggt agtgttattc atatctcact ggtcaactta ttaagtacac ctatctcaaa
      601 cattggtttt aatgcgacat ggggtggtga aaaacctgtc gatgccaaag agtttgcccg
      661 gtggcaacaa ttacttttca acacatcaat gaaatccaca ttgaaattat taccaggtca
      721 atggcaagac attaatttga ccctgaaggg tgtatcgccc aataacctgg gatatctgaa
      781 gttagccatc aacatggaaa atattcagtt cgacaatctt ccctctgctg aaaatcggca
      841 gaaaagaagc aaaaaataaa tgcagttagt acgcttataa caaggaatag cgagcatcat
      901 taatgaacaa aatatttaaa gttatctgga accctgcgac agggaattat actgttacca
      961 gcgaaacggc aaaaagccgt ggcaagaaat ctgggcgcag taagctgtta atttctgcgc
     1021 tggttgcggg tggaatgttg tcgtcgtttg gggcattggc gaatgccggg aatgacaacg
     1081 gtcagggtgt tgattacggt agtggatcag ctggcgacgg ctgggttgct ataggcaaag
     1141 gggcgaaagc aaatactttt atgaacacca gtggttccag tactgctgtg ggttatgacg
     1201 ctatagctga aggccaatat agctctgcca tcgggtcaaa aacccatgcg attggtggtg
     1261 catcaatggc ctttggggtt agtgcaatat cagaaggcga tagaagtata gcactgggtg
     1321 cctcttcgta ttcattgggc caatactcaa tggccctcgg ccgttattca aaagcattgg
     1381 gtaaattgtc tattgctatg ggggactctt ccaaagcgga aggagcaaac gccattgccc
     1441 tggggcgtag cagtgtagct agcggtactg acagcctcgc atttggcaga caatcacttg
     1501 ccagcgcagc gaacgctatt gcgataggtg ctgagaccga agccgctgaa aatgcaactg
     1561 ctattggcaa taatgcgaag gcaaaaggga ctaatagcat ggcaatgggg ttcggaagcc
     1621 ttgccgataa agtcaatact atcgcattag gaaatggcag ccaggctctg gcagataatg
     1681 caatcgccat aggccagggc aacaaagctg atggcgtgga tgccatcgct ctgggtaatg
     1741 gtagccagtc gagaggctta aacaccattg ccttaggcac agccagtaat gcaactggtg
     1801 ataagagtct tgcgcttggt agtaatagca gtgccaacgg tattaactct gtcgcgctgg
     1861 gcgcagattc cattgcggat ttagacaata ccgtctctgt cggcaatagt tcattaaaac
     1921 gcaagatcgt taatgtgaaa aatggcgcga tcaagtctga cagttacgat gccattaatg
     1981 gttcacagct ttatgccatt agcgactcgg tagcaaaaaa ggcttggagg aggggctgca
     2041 gtagatgttg atgacggtac tgttacagca ccaacctaca atttaaaaaa tggtagcaaa
     2101 aataacgtag gggctgcgct cgctgtactt gatgaaaaca ccctgcaatg ggaccaaacc
     2161 aaaggcaaat acagcgctgc tcatggtact agtagcccaa ctgccagcgt aatcaccgat
     2221 gttgcggatg gcacgatttc agcctccagt aaggatgcgg ttaacggttc ccaactgaaa
     2281 gctaccaatg acgatgtcga agccaacacc accaatatca ccaacctgac ggattccgtt
     2341 ggtgaccttc aggctgatgc cctgctctgg aacgaaacta aaaaggcatt cagtgcagct
     2401 cacggccagg ataccaccag caaaatcacc aacgttaaag atgccgacct gacggctgac
     2461 agcactgatg ctgttaacgg ctctcagctg aaaaccacca acgatgctgt ggcgacgaat
     2521 accaccaata tcgccaataa cacttccaat attgccacta acaccaccaa catctctaac
     2581 ctgactgaga cggtgactaa tcttggtgag gatgcgctga aatgggataa ggacaatggt
     2641 gtattcacgg cagctcatgg caccgagacc accagcaaaa taaccaacgt taaagatggc
     2701 gacctgacga ctggcagcac cgatgccgtt aacggctctc agctgaaaac caccaacgat
     2761 gccgtggcga cgaataccac caatatcgcc actaacacca ccaacatctc taatctgact
     2821 gagacggtga ctaatcttgg tgaggatgcg ctgaaatggg ataaggacaa tggtgtcttc
     2881 actgcagctc atggcaacaa taccgccagc aaaatcacca atatcctgga cggcacagtc
     2941 actgcaacca gttccgatgc cattaacggt agccagcttt atgacttaag cagcaatatc
     3001 gccacctact tcggcggcaa tgcttctgtg aatactgacg gtgtgtttac cggtccaacc
     3061 tacaaaatcg gtgaaacaaa ttattataac gtcggcgatg cactggctgc gattaactcc
     3121 tcatttagca cgtctctcgg cgatgctctg ctttgggatg ccaccgcagg taaattcagt
     3181 gccaaacacg gtactaatgg tgacgcaagc gtgatcactg atgtcgcaga tggtgaaatt
     3241 tcagactcca gttctgacgc agtaaacggc tcacaactcc acggcgtgag cagttatgtt
     3301 gttgatgcgc tggggggtgg tgccgaagtc aatgcagacg gcaccatcac tgcgccgacg
     3361 tacaccattg ctaatgctga ttacgataat gtcggtgatg ccctgaatgc tatcgatacc
     3421 actcctgacg acgctctgct ctgggatgcg gacgccggtg aaaatggtgc atttagcgcc
     3481 gctcacggaa aagataaaac tgccagtgta atcactaacg tcgctaacgg tgcaatctct
     3541 gctgccagca gcgacgcgat taacggctca caactctata ccaccaataa gtacatcgct
     3601 gatgcgctgg gtggtgacgc agaagtcaac gctgacggca ccatcaccgc accgacttac
     3661 accattgcga acgccgagta caacaacgtc ggtgacgccc tggatgcgct tgatgataac
     3721 gccctgctgt gggataagac tgccaatggc ggtgctggag cctacaatgc cagccatgac
     3781 ggtaaagcca gcatcatcac taatgtcgct aatggcagta ttagtgagga cagtaccgat
     3841 gcagtgaacg gttctcagtt gaatgcgacg aatatgatga ttgagcagaa cacccaaatt
     3901 atcaatcagc tcgctggtaa caccgacgca acctatatcg aagaaaatgg cgctggtatc
     3961 aactacgttc gtactaacga caacgattta gcctttaacg atgcaagcgc ctctggtgtc
     4021 ggcgctacag ctgtaggtta taacgctgtc gcgtctggtg ccagcagcgt agccattggt
     4081 cagaacagca gcagcaccgt tgataccggt attgcgctgg gtagcagctc cgtttccagc
     4141 cgtgtgattg ccaaaggttc tcgtgacact agcgtaacgg aaaatggcgt ggtgattggt
     4201 tatgacacca ctgacggcga actgctaggt gcattgtcaa ttggtgatga cggtaaatac
     4261 cgccaaatca tcaacgtagc tgatggttca gaagcccatg acgccgttac ggttcgccag
     4321 ttgcagaacg ctattggagc ggtcgccact acgccaacca agtactttca cgccaactca
     4381 acggcagaag actcactggc cgttggtgaa gactcgctgg caatgggtgc gaaaactgtc
     4441 gttaatggta atgcaggtat tggtatcggt ttgaacactc tggttctggc tgatgcgatc
     4501 aacggcattg ctatcggcag taacgcacgc gcaaaccatg caaacagtat cgcaatgggt
     4561 aacggttctc agaccacccg tggtgcacag actggctaca ccgcctacaa catggacgca
     4621 ccgcagaact ctgtaggtga gttttctgtc ggtagtgaag acggtcaacg tcagatcaca
     4681 aacgtcgcag ctggttcagc ggataccgat gcggttaacg tgggtcagtt gaaagtcact
     4741 gatgagcgcg tagcgcaaaa tacccagagc attactaacc tgaacaatca ggtcactaat
     4801 ctggatactc gcgttactaa tatcgaaaac ggtattggcg acattgtcac caccggtagc
     4861 accaagtact tcaagaccaa caccgatggc gtagatgcca acgcccaggg taaagatagc
     4921 gttgctattg gttctggttc cattgctgcc gctgacaaca gcgtcgcact gggtaccggt
     4981 tccgttgcag aggaagaaaa tacaatctct gtaggttctt ctactaacca acgccgtatc
     5041 accaacgtag ctgccagtgt taatgccacc gatgcggtta acgtgtcgca gctgaaatct
     5101 tctgaagcag gcggagtgcg ctacgacacc aaagctgatg gttctatcga ctatagcaat
     5161 atcaccctcg gtggtggcaa cggcagtacg actcgtatca gcaacgtctc cgctggcgtc
     5221 aacaacaacg acgcggtgaa ctacgcgcag ttgaagcaaa gcgcgcagga aacgaagcaa
     5281 tacaccgatc agcggatggt tgagatggat aacaaactgt ctaaaactga aagcaagttg
     5341 agcggtggta tcgcttctgc aatggcaatg accggtctgc cgcaggctta tacaccgggt
     5401 gccagcatgg cttctattgg tggcggtact tacaacggtg aatcggcagt tgctttaggt
     5461 gtatcgatgg tgagcgccaa tggtcgttgg gtctacaaat tacaaggtag taccaatagc
     5521 cagggtgaat actccgccgc actcggtgcc ggtattcagt ggtaatgatc cattaagtta
     5581 gtgtgactaa gggcaaacgc caaattgcct gatgcgttac gcgcatcagg caagataagg
     5641 cgctccgggt caacaatcta agggcaattc tctgatgagg attgcccttt tctttaccag
     5701 acatctcccc ccacaagaat tggccctacc aattcttcgc ttatctgacc tctggttcac
     5761 aattccccaa ttaaaactca catcaatatt gccaatacat aacatttagt taaccattca
     5821 ttgtcattat ccctacacaa cacaattggc agtgccactt ttacacaacg tgtgacaagg
     5881 agatgagcaa cagactcatt acacgatgtg cgtggactct aggagacctg caatgaatct
     5941 ctggcaacaa aactacgatc ccgccgggaa tatctggctt tccagtctga tagcatcgct
     6001 tcccatcctg tttttcttct ttgcgctgat taagctcaaa ctgaaaggat acgtcgccgc
     6061 ctcatggacg gtagcaatcg cccttgccgt ggctttgctg ttctataaaa tgccggtcgc
     6121 taacgcgctg gcctcggtgg tttatggctt cttctatggt ttgtggccca tcgcgtggat
     6181 cattattgca gcagtgttcg tctataagat ctcggtgaaa accgggcagt ttgacatcat
     6241 tcgctcgtct attctttcga taacccctga ccagcgtctg caaatgctga tcgtcggttt
     6301 ctgtttcggc gctttccttg aaggagccgc aggctttggc gcaccggtag caattaccgc
     6361 cgcattgctg gtcggcctgg gttttaaacc gctgtacgcc gccggactgt gcctgattgt
     6421 taacaccgcg ccagtggcat ttggcgcgat gggcattcca atcctggttg ccggacaagt
     6481 aacaggcatc gacagctttg agattggcca gatggtgggg cgtcagctac cgtttatgac
     6541 cattatcgtg ctgttctgga tcatggcgat tatggacggc tggcgcggta tcaaagagac
     6601 gtggcctgcg gtcgtggttg caggcggctc gtttgccatc gctcagtacc ttagctctaa
     6661 cttcattggg ccggagctgc cggacattat ctcttcgctg gtatcactgc tctgcctgac
     6721 gctgttcctc aaacgctggc aaccagttcg cgtattccgc ttcggtgact tgggggcgtc
     6781 acaggttgat atgacgctgg cccacaccgg ttacaccgcg ggtcaggtgt tacgtgcctg
     6841 gacaccgttc ctgttcctga ccgctaccgt aacactgtgg agcatcccgc cgtttaaagc
     6901 cctgttcgca tcgggtggtg cgctgtatga gtgggtgatc aacattccgg tgccgtacct
     6961 caataaactg gttgcccgta tgccaccagt ggtcagcgag gctacagcct atgccgccgt
     7021 gtttaagttt gactggttct ctgccaccgg caccgccatt ctgtttgctg cactgctctc
     7081 gattgtctgg ctgaagatga aaccgtctga cgctatcagc accttcggca gcacgctgaa
     7141 agaactggct ctacccatct actccatcgg catggtgctg gcgttcgcct ttatctcgaa
     7201 ctattccgga ctgtcatcaa cactggcact ggcactggcg cacaccggtc atgcattcac
     7261 cttcttctcg ccgttcctcg gctggctggg ggtattcctg accgggtcgg atacctcatc
     7321 taacgccctg ttcgccgcgc tgcaagccac cgcagcacaa caaattggcg tctctgatct
     7381 gttgctggtt gccgccaata ccaccggtgg cgtcaccggt aagatgatct ccccgcaatc
     7441 tatcgctatc gcctgtgcgg cggtaggcct ggtgggcaaa gagtctgatt tgttccgctt
     7501 tactgtcaaa cacagcctga tcttcacctg tatggtgggc gtgatcacca cgcttcaggc
     7561 ttatgtctta acgtggatga ttccttaatg attgttttac ccagacgcct gtcagacgag
     7621 gttgccgatc gtgtgcgggc gctgattgat gaaaaaaacc tggaagcggg catgaagttg
     7681 cccgctgagc gccaactggc gatgcaactc ggcgtatcac gtaattcact acgcgaggcg
     7741 ctggcaaaac tggtgagcga aggcgtgctg ctcagtcgac gcggcggcgg gacgtttatt
     7801 cgctggcgtc atgacacatg gtcggagcaa aacatcgtcc agccgctaaa aacgctgatg
     7861 gccgatgatc cggattacag tttcgatatt ctggaagccc gctacgccat tgaagccagc
     7921 accgcatggc atgcggcaat gcgcgccaca cctggcgaca aagaaaagat tcagctttgc
     7981 tttgaagcaa cgctaagtga agacccggat atcgcctcac aagcggacgt tcgttttcat
     8041 ctggcgattg ccgaagcctc acataacatc gtgctgctgc aaaccatgcg cggtttcttc
     8101 gatgtcctgc aatcctcagt gaagcatagc cgtcagcgga tgtatctggt gccaccggtt
     8161 ttttcacaac tgaccgaaca acatcaggct gtcattgacg ccatttttgc tggtgatgcc
     8221 gacggggcgc gtaaagcaat gatggcgcac cttagttttg ttcacaccac catgaaacga
     8281 ttcgatgaag atcaggctcg ccacgcacgg attacccgcc tgcccggtga gcataatgag
     8341 cattcgaggg agaaaaacgc atgattattt ccgcagccag cgattatcgc gccgcagcgc
     8401 aacgcattct gccgccgttc ctgttccact atatggatgg tggtgcatat tctgaataca
     8461 cgctgcgccg caacgtggaa gatttgtcag aagtggcact gcgccagcgt attctgaaaa
     8521 acatgtccga cttaagcctg gaaacgacgc tgtttaatga gaaattgtcg atgccggtag
     8581 cactggcacc ggtgggtttg tgtggcatgt atgcgcgtcg tggcgaagtt caggcagcca
     8641 aagcggcgga cgcgcatggc attccgttta ctctctcgac ggtttccgtt tgcccgattg
     8701 aagaagtcgc gccagccatc aagcgcccaa tgtggttcca gctttatgta ctgcgcgatc
     8761 gcggctttat gcgtaacgcg ctggagcgag caaaagcagc gggttgttcg acgctggttt
     8821 tcaccgtgga tatgccgaca ccgggcgcac gctaccgtga tgcgcattca ggtatgagcg
     8881 gcccaaatgc ggcaatgcgc cgctacttgc aggcggtgac gcatccgcaa tgggcgtggg
     8941 atgtgggcct gaacggtcgt ccacatgatt taggtaatat ctcagcttat ctcggcaaac
     9001 cgaccggact ggaagattac atcggctggc tggggaataa cttcgatccg tccatctcat
     9061 ggaaagacct tgagtggatc cgcgatttct gggatggccc gatggtgatc aaagggatcc
     9121 tcgatccgga agatgcgcgc gatgcagtac gttttggtgc tgatggaatt gtggtttcta
     9181 accacggtgg ccgccagctg gacggtgtac tctcttccgc tcgtgcgttg cccgctattg
     9241 cagatgcggt gaaaggtgat atcgccattc tggcggatag cggaattcgt aacgggcttg
     9301 atgtcgtgcg tatgattgcg ctcggtgccg acaccgtact gctgggtcgt gctttcctgt
     9361 atgcgctggc aacagcgggc caggcgggtg tagctaacct gctaaatctg atcgaaaaag
     9421 agatgaaagt ggcgatgacg ctgactggcg cgaaatcgat cagtgaaatt acgcaagatt
     9481 cgctggtgca ggggctgggt aaagagttgc ctacggcact ggctccgatg gcgaaaggga
     9541 atgcggcata gtcgtttgcg ccccctcacc ctaaccctct ccctcaggga gaggggaccg
     9601 ttcggcgctg tatgtactcc ctcactctga aacgacaccg cactcttttt ttctccctcg
     9661 cccctccggg gagggccggg gtgaggggaa aaggccgcac ggttcaccaa ttttctgcta
     9721 tcctgccccc gcactaaggg ggcagtatgc taaacatcgt actttacgaa ccagaaattc
     9781 cgccaaatac tggcaacatc atccgtcttt gcgctaatac cggctttcgt ctacatatca
     9841 tcgaaccgat gggatttgcc tgggacgaca agcgcctgcg ccgcgcgggg ctggactatc
     9901 acgagtttac cgccgttacg cgtcatcatg actatcgcgc gttcctcgaa gcagaaaatc
     9961 cccagcgcct gtttgccctc accacgaaag gtacgcctgc tcacagcgcc gtaagctatc
    10021 aggatggcga ttatctgatg ttcggcccgg aaacacgcgg cctgccagcg agcattcttg
    10081 atgccctgcc cgctgaacaa aaaattcgca ttccgatggt gccggacagc cgcagcatga
    10141 atctgtccaa tgcggtgtcg gtagtggtgt atgaagcctg gcggcagctg ggatatccgg
    10201 gagcgttgtt gagagattag ttactgtatg ccggatagat gattacatcg catccggcac
    10261 gatcacagga cattagatcc catccccata ctcaaatgta tggttaatac cgttgaaatg
    10321 ctggtccata tccattgatg gcttatcgct gtctggttta ccgacaatac gagccggaac
    10381 gccagcggcg gttgtatgcg gcggcaccgg ttgcagcacc acggaacctg cgccaatctt
    10441 cgcgccgcaa ccaacttcaa tattgccgag gattttcgcg cccgcgccaa tcatcacacc
    10501 ttcacgaatt ttcgggtgac ggtcaccacc agatttaccc gtaccgccaa gcgtcacaga
    10561 ttgcagaatc gatacgtcgt tttcaattac cgccgtttca cccacaacga tgcccgtggc
    10621 gtggtcgagc ataatgccac ggccaatttt tgctgccggg tgaatatcga cctggaacgt
    10681 cacagaaacc tggttttgca gaaagattgc caatgcgcga cgcccctgat tccacaacca
    10741 gtgaccgatg cgataggcct gcaaggcatg aaaacccttc aggtataaca acggggttga
    10801 gtatttatcg actgccgggt cgcgggtacg caccgcctga atatcacagg ccgcagaggc
    10861 gatcatttcc gggtcagcgg cgtaggcttc ttcaaccact tcacggatag caatagcagg
    10921 cataattggc gatgacagct tgttcgccag catgtagctc agtgcactgc caaggttttc
    10981 gtgcttgagt agcgtcgcgt ggtaaaaact ggccagcatt ggctcacagt ccgccagcgt
    11041 tctggcttcg gctttaatat tgttccagac aatttccagt tcttcacacg acattgctta
    11101 ctccacacga tgagataatg accggcccgt tctgcgcggg ccgggtcata gcggtaacaa
    11161 aggttccctg gggttagtga ctgctgcgct cgtccttgcg tgcacgacct aataatgtca
    11221 atgctgcctc gcgcgcgttt tttccgcaat ataatacttg ataaatttcc tcggttattg
    11281 gcatttcaac gccgaagcga tgcgccagtt cgcggacttc tttcgtattg cggtagcctt
    11341 ccaccacctg accaatcttc tcctgcgcgc tttgtacatc catgccctga ccgagcatca
    11401 taccaaaacg gcggttacgc gactggttgt cggtacaggt aagcaccaga tcgcccagcc
    11461 ccgccatgcc cataaaggtg gcaggatcgg cacccagcgc cgcaccaaga cgcgacattt
    11521 cagccagccc acgggtgatc agcgccgtac gcgcattcgc accaaaaccg ataccgtcag
    11581 acatccccgc gccaatggca atgacgtttt tcaccgcgcc gccaagctgc acgccaatga
    11641 aatccgggtt gctgtaaacg cggaaacttt tgccgcagtg cagcagctgc tggagatcat
    11701 cggcaaaggt ctgatcggtc gaggccagcg aaatagctgt cggtaaacct gccgccagtt
    11761 ctttcgcaaa cgttgggcca gagataaccg ccagcggaat ttgatcgcct aaggcctcac
    11821 gcgccacgtc ctgtaacaga cgtccggttt ccgcttccag ccctttggtc gcccacacca
    11881 gacgcgcatc aggacgcatc agcggtttaa tctggcgcag cacttcacca aagacatggc
    11941 tgggtacgac gacgagaata ttacgactgg ctgccagcgc agtggcgaga tcgctttcaa
    12001 gatggagcgt atcgggaaaa ggcacatcgg ggagaaacgc ggcgttacag cggtcgcgtt
    12061 caagcgttgc gatatgttca gggtcatggc cccagaggac aacctcgtgg ccatttcttg
    12121 ccagggtaat ggcaagagcg gtgccgtacg agccggcacc gatcacagtc attgaagcat
    12181 tacgttggtt catcaggcat cctgatgttc ttcagtacct tcgccagcct gctgctgcaa
    12241 atagttcatg aacagcgcat cgaagttaac cggcgcaagg ttcagttgcg ggaatgtacc
    12301 gcgggatacc atgctggtga tgcactcacg agcatacggg aacagaatgt tcgggcagta
    12361 tgcgcccagg caatgtgcca tctgggtgcc ttcgataccc gcgatggaga aaataccgcc
    12421 ctgctgaact tcacacagga acgcggtttc ttcgcccaag gaggccgtta cggtaacacg
    12481 cagtaccact tcgtatacgt catctgccag ttgggaagat gccgtatcca gatcaagttt
    12541 aacttctggt tgccaatctt tctggaaaac gtgcggcgcg ttcggcgctt cgaaagagat
    12601 atccttggta taaatacgtt ggatctggaa agtcatttca gtgttgtttt gttctgacat
    12661 gtgtagaaaa cccttaagtg ttgtccttaa atactgcgca atgtaatacc aacgttcaca
    12721 cgttatttca gcaggggatc cagtccacca cgtgcatcca atgcatacaa gtcatcacag
    12781 ccgccaatgt gctgtgcgtc aataaaaatc tggggaaccg tggtgcaacc gctgcgtttg
    12841 atcatctctt cacgcttggc ggcattgcca tcgatcggca actcctggaa actcacgccc
    12901 ttgctgctca gcagtgcttt tgcacgatgg caatacgggc aggtttcttt ggtatagatt
    12961 tcaacattgg ccataattta gctcctgttt ttttaccctg aaatttcatg ttgcagggcg
    13021 gctgcaaaat ggagtgtccc cagaagctta taacaataag tcgctggggg cctaattgca
    13081 gctaacggcc tgcatcatga aagacgacag gtaaattatt tgccgcgcac cagaggcaag
    13141 ttttcgccag cccagccagc aacgccctct ttcagcacga atacttgcgc aaaaccagct
    13201 ttcgtcagcg cgtttgcagg ctcctggcac tgcatgccag aaccgtctac cacgataacc
    13261 gatttgtctt tgtgcttctc aagctcacca acattgttgg ctttgatttc gctcggcaac
    13321 agattgatag aacctgcgat atggccttta cggaagtcat cacgctgacg taaatccaca
    13381 accacagcgt cttctttgtt gatcagacgc gtagcttcac cacgagtaat caccttcact
    13441 ttcgaggtca ggctcttaaa cgtagtcaca agaaccgcca ccagtaacgc gatccaggcg
    13501 atactcagta tgggatgacg gccaacaaat tgcataattt cttgcatggg gggtaacaac
    13561 tcccgacgta gtgattaaaa aaaccaggaa aggagtatac ctgcgcggtg cggcaaatac
    13621 agccagcgcg ttaactggaa tgcaattttg cggggcacga cgaaaaaaaa gcgcaaatct
    13681 ggcatcggct tgccgcgagc ggtcgtatat tttgatcttc agaggctatt ttatcgattc
    13741 agctgtagta aaattacgca aattttgact cttgagtatg aggttgtcgc aatgtcggtt
    13801 tctaaaaaac ctatggtact ggtgattctg gatggctatg gctatcgtga agaacagcag
    13861 gataacgcca tttttagtgc taaaaccccg gtaatggatg cactgtgggc caatcgtccg
    13921 cataccctga tcgacgcttc cggtctggaa gtcggtctgc ctgaccgtca gatgggtaac
    13981 tccgaagtag gccacgttaa cctgggtgcc ggccgcatcg tgtatcagga cctgactcgt
    14041 ctggacgttg aaatcaaaga tcgcgctttc tttgctaatc cagtgctggc tggcgcggta
    14101 cataaagcga aaaacgcagg taaagcagta cacattatgg gtctgctctc cgcaggcggc
    14161 gtacacagcc acgaagatca catcatggcg atggtagaac tggcagctga acgcggcgca
    14221 gaaaaaatct acctgcacgc attccttgac ggtcgcgaca ccccgccgcg cagtgctgaa
    14281 tcctcgctga aaaaatttga agagaaattt gctgcgctgg gtaaaggccg cgtagcgtcc
    14341 atcattggtc gttactacgc gatggaccgc gataaccgtt gggatcgcgt tgaaaaagcc
    14401 tatgacctgc tgactctggc gcagggtgag ttccaggccg atactgccgt tgctggtttg
    14461 caggcagctt atgctcgcga cgaaaacgac gagttcgtga aagcgaccgt tattcgtgca
    14521 gaaggtcagc cagatgcggc aatggaagac ggcgatgcgc tgattttcat gaacttccgt
    14581 gctgaccgcg cgcgtgaaat cactcgtgca ttcgtgaacg ctgatttcga tggcttcgcg
    14641 cgtaagaaag tggttaacgt cgatttcgtg atgctgaccg aatacgccgc tgacatcaaa
    14701 actgcggttg cttacccacc cgcttccctg gttaacacct tcggcgagtg gatggcgaaa
    14761 aacgacaaaa ctcagttgcg tatttccgaa accgaaaaat atgcccacgt tactttcttc
    14821 ttcaacggtg gcgtagaaga gtcgttcaaa ggcgaagatc gcattctgat caactcaccg
    14881 aaagtggcta cctacgatct gcaaccggaa atgagctccg cagagctgac cgaaaaactg
    14941 gttgcggcca tcaagagcgg caaatacgac accattatct gtaactatcc gaacggcgac
    15001 atggtaggtc acaccggggt gatggaagcg gcggttaaag cggttgaagc actggatcac
    15061 tgcgtggaag aagtcgcgaa agcggttgag tccgtgggtg gacaactgct gatcaccgct
    15121 gaccacggta acgctgaaca gatgcgcgat ccggcaacgg gtcaggcaca cacggcacac
    15181 accaacctgc cagttccgct gatttacgtt ggtgataaga acgtgaaagc ggttgcaggc
    15241 ggcaaacttt ctgacatcgc gccgaccatg ttgtcgctga tgggtatgga aatcccgcaa
    15301 gagatgactg gtaagccgct gttcatcgtg gaataatccc tccccatgag gggaaaggcg
    15361 attaatacca tgacacgggc cgtgaaaccg cgcaggtttg caatcaggcc catcatctac
    15421 gccagcgttc ttagcgctgg cgtattgttg tgcgcctttt ccgcccacgc ggatgagcgt
    15481 gaccaactca aatctattca ggccgatatc gccgcaaaag agcgcgcggt acgccaaaag
    15541 caacaacaac gcgcaagcct gctcgcacaa ttgaaaaagc aggaagaagc gatctctgaa
    15601 gccacccgta agctgcgcga aacgcaaaac acgctcaatc aactcaataa acagattgat
    15661 gagatgaacg cgtcgattgc caaactggag cagcaaaaag ccgcccagga gcgcagcctc
    15721 gccgcacaac tggatgccgc attccgtcag ggcgagcata ccggtattca gctaattctc
    15781 agcggtgaag aaagccagcg tggacagcgt ttacaggctt atttcggcta tctcaaccag
    15841 gcgcgacaag aaaccattgc ccagttgaag caaacgcgtg aagaagtcac catgcagcat
    15901 gctgaactgg aagagaaaca aagcgagcaa caaacgctgt tatatgaaca gcgcgcccaa
    15961 caggcgaaac tgactcaggc tctgaacgag cgtaaaaaga cgctggcggg gctggaatct
    16021 tccatccagc aaggtcagca gcagctaagt gaactgcgtg ccaacgaatc ccgtctgcgt
    16081 aacagcattg cccgtgctga agccgcggcg aaagcgcgtg ctgaacgaga agcgcgtgag
    16141 gcccaggcgg ttcgcgagcg ccagaaagaa gcgacgcgca aaggcaccac ctacaaaccg
    16201 accgaaagcg aaaaatcgct gatgtcccga actggtggcc tgggtgcgcc gcgtggtcag
    16261 gcattctggc cggttcgcgg gccgacgctg catcgctatg gcgagcagct acagggtgaa
    16321 ttacgctgga aagggatggt tatcggtgcc tctgaaggta ctgaagttaa agcaattgcc
    16381 gacggtcgag tgattctggc tgactggctg caaggttacg gtctggtggt ggtggttgag
    16441 cacggtaaag gcgacatgag tctttacggc tataatcaga gcgcactggt gagcgttggt
    16501 tcgcaggttc gcgcgggcca gccaattgca ctggtgggca gcagtggcgg tcagggtcgg
    16561 ccttcactct atttcgaaat tcgccgccag ggtcaggcgg tcaatccaca gccgtggttg
    16621 ggaagataag ttttgtttcc atttcgtcgt aacgttcttg catttgccgc tctgttggcg
    16681 ctctcctccc ccgtacttgc tggcaaactt gccatcgtca ttgatgattt tgggtatcgc
    16741 ccgcacaacg aaaaccaggt gctggcgatg ccttccgcta tctccgtcgc tgtattaccc
    16801 gattcaccgc acgccagaga gatggcgacc aaagcgcata acagcggtca cgaagtgttg
    16861 attcatctcc cgatggcacc gttgagtaaa cagccgctgg agaaaaatac gctacgcccg
    16921 gagatgagca gcgacgaaat tgagcgcatt attcgtagtg cggtcaataa cgtgccctat
    16981 gccgtgggga tcaacaacca catgggcagc aagatgacct ctaacctgtt tggtatgcag
    17041 aaagtgatgc aggcgctgga gcgttacaat ctttacttcc tcgacagcgt aaccatcggt
    17101 aatacccagg cgatgcgcgc cgcgcaaggt actggcgtga aggtgatcaa acggaaggtg
    17161 ttcctcgacg attcgcagaa cgaagcggac atccgtgtgc aatttaatcg cgcaattgat
    17221 ctggcgcgtc gcaacggttc aaccattgcc attgggcatc ctcacccttc aacggtacgc
    17281 gtgctacaac agatggttta taacctgccg ccagacatta cgctggtgaa agccagcagc
    17341 ttgctgaatg aaccgcaggt tgatacttct acaccgccga aaaacgctgt gcctgatgca
    17401 ccgcgtaatc cgttccgtgg cgtgaagctg tgcaaaccga agaaaccgat agaacctgtt
    17461 tatgctaatc gcttctttga agtattaagc gaaagcatca gccagagcac gctgatcgtt
    17521 tacttccagc atcagtggca aggctggggc aaacagcccg aagcggcgaa gtttaacgct
    17581 agcgcaaatt aaaggcgcta cgcgtaatgc gatggtgcga ccgcgttttg tcgcgccatt
    17641 gccataagcg gaaagaccac agtaacgcct ggtaaccgac cttcacgctg cgcacattgg
    17701 taatcaggcg cttatacata cccggaagta aatatctcgg caatcatccg ttggcgggta
    17761 agaatatccg gctctttgcg
//
LOCUS       DAZSIO010000078        17643 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZSIO010000078 DAZSIO010000000
VERSION     DAZSIO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.078"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(1..671)
                     /gene="chbR"
                     /locus_tag="ACHX5T_002366"
                     /pseudo
     CDS             complement(1..671)
                     /gene="chbR"
                     /locus_tag="ACHX5T_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="ACHX5T_002367"
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="ACHX5T_002367"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR8467540.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="ACHX5T_002368"
                     /pseudo
     CDS             complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="ACHX5T_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="ACHX5T_002369"
     CDS             complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="ACHX5T_002369"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR8467541.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="ACHX5T_002370"
     CDS             complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="ACHX5T_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR8467542.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3680..4507
                     /gene="nadE"
                     /locus_tag="ACHX5T_002371"
     CDS             3680..4507
                     /gene="nadE"
                     /locus_tag="ACHX5T_002371"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR8467543.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4737..5624
                     /gene="cho"
                     /locus_tag="ACHX5T_002372"
     CDS             4737..5624
                     /gene="cho"
                     /locus_tag="ACHX5T_002372"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR8467544.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5584..6159)
                     /gene="ves"
                     /locus_tag="ACHX5T_002373"
     CDS             complement(5584..6159)
                     /gene="ves"
                     /locus_tag="ACHX5T_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR8467545.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6362..6847)
                     /gene="spy"
                     /locus_tag="ACHX5T_002374"
     CDS             complement(6362..6847)
                     /gene="spy"
                     /locus_tag="ACHX5T_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR8467546.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7177..8145)
                     /gene="astE"
                     /locus_tag="ACHX5T_002375"
     CDS             complement(7177..8145)
                     /gene="astE"
                     /locus_tag="ACHX5T_002375"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR8467547.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(8149..9470)
                     /gene="astB"
                     /locus_tag="ACHX5T_002376"
                     /pseudo
     CDS             complement(8149..9470)
                     /gene="astB"
                     /locus_tag="ACHX5T_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9467..10945)
                     /gene="astD"
                     /locus_tag="ACHX5T_002377"
     CDS             complement(9467..10945)
                     /gene="astD"
                     /locus_tag="ACHX5T_002377"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFR8467548.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10942..11976)
                     /gene="astA"
                     /locus_tag="ACHX5T_002378"
     CDS             complement(10942..11976)
                     /gene="astA"
                     /locus_tag="ACHX5T_002378"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR8467549.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11973..13193)
                     /gene="astC"
                     /locus_tag="ACHX5T_002379"
                     /pseudo
     CDS             complement(11973..13193)
                     /gene="astC"
                     /locus_tag="ACHX5T_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13639..14445
                     /gene="xthA"
                     /locus_tag="ACHX5T_002380"
     CDS             13639..14445
                     /gene="xthA"
                     /locus_tag="ACHX5T_002380"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR8467550.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14612..15322
                     /locus_tag="ACHX5T_002381"
     CDS             14612..15322
                     /locus_tag="ACHX5T_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR8467551.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            15327..16004
                     /gene="ydjY"
                     /locus_tag="ACHX5T_002382"
     CDS             15327..16004
                     /gene="ydjY"
                     /locus_tag="ACHX5T_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR8467552.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            16019..16726
                     /locus_tag="ACHX5T_002383"
     CDS             16019..16726
                     /locus_tag="ACHX5T_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR8467553.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16726..17274
                     /locus_tag="ACHX5T_002384"
     CDS             16726..17274
                     /locus_tag="ACHX5T_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR8467554.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            17284..17610
                     /locus_tag="ACHX5T_002385"
                     /pseudo
     CDS             17284..17610
                     /locus_tag="ACHX5T_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         4365 a         4685 c         4308 g         4285 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactacg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtcc gctgatactc tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttcca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     1441 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagc
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     2161 ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     2221 tggttgaggc gtcgaggcga atacctaagg aataaaaaac gacccctccc caaagcttag
     2281 aaaaacgttg ttaattaata caaacatggc ccccgcaagg gttaacggca ttaagcgaat
     2341 aaagccattt ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag
     2401 gagtaccttt tcaagcgatg caataacatt actcatagaa aaataccctt aaaaaccgca
     2461 atttaaatat tacggtattg atttatgaaa taactctttg acgggaaaat ttaaaataaa
     2521 ttaatttgct gcggcttttt taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc
     2581 gactttgcca taaagcagcg agtcaattac ttcaaccggt ttgttgggta acaaacgctg
     2641 gatttcgggc aacatataag caatctgcgg ccctaataac acgacatcgg cattctgacc
     2701 tttttcacca gccagtgttt ccggaaatgc ttcaataatg accggaactt catatttttc
     2761 tgcctgtgcg cgcatttttg ataccagtaa agagatagac atgcccgcag aacaaaacag
     2821 ataaatgtgt ttcttttcca taaaactgcc ctcatcgacg attatctgtc agccagacac
     2881 tccgcaagcc ttgacctgct tccatgctct gggtaacttg cgaaaccaac atgacttttt
     2941 ttgtggctga aaggagtata cggtatagac ctgagcaata gtatttcttt gaccttataa
     3001 attgtttctc cttgacctgt ggttatgacc ctcttcacat ttcgggcaaa taattcgcga
     3061 agataattaa gaactcaggc caaaaaaaac cggcgcaatg gccggtttcc gttgttactc
     3121 aagctttcag acgaattgat tacttcgcag cctgtggatc agtgtcgtat tcagcacagg
     3181 tctgataacc ggagttgatg acatgtccgg tatcatctaa cgcgacaaag taggtttctg
     3241 ctttaccatc acgttgaccc aggatgtagg tctggcaagt accgcgagca tggatcatgc
     3301 tcacttcaga cgaaggttta cccgcaattt gtgcaacctg cgcccggctc atgccttttt
     3361 tgacgtcttt caccacaggc tgtacaaact ggtctttggt acgatcataa gctgtacaac
     3421 ccgccagcat ggttaatacc gccgctgcac tcagaattcc tgccatattc ttgttcatat
     3481 tccgtcctct tgtttatcag cgtgttagat aagcctggaa tacattgggc gctttttcaa
     3541 gcccgtgaac gaaacggctc cgctttcaga ggattcctgt atgacgtttt aaccaccatt
     3601 cagcccgctg tcgcttgtcg tttcagtagc aacgggttag ctttaaggaa gttttgtctt
     3661 ttctgtctgg aggggttcaa tgacattgca acaacaaata ataaaggcgc tgggcgcaaa
     3721 accgcagatt aatgctgaag aggaaattcg tcgtagtgtc gattttctga aaagctacct
     3781 gcaaacttat ccgttcatta aatcactggt gctcgggatc agcggcggtc aggactccac
     3841 gcttgccgga aagctgtgcc agatggcgat taatgagctg cgccaggaaa ctcgcaacga
     3901 atcactgcaa tttattgccg tacgcctgcc ctatggtgtt caggctgacg aacaagattg
     3961 ccaggatgcc attgccttta ttcaaccgga tcgcgtatta accgttaata tcaagggcgc
     4021 ggtattggct agcgagcagg cattgcggga agcaggcatt gaactgagcg attttgtccg
     4081 tggcaatgaa aaagcgcgtg agcggatgaa agcacaatat agcattgcgg gtatgaccag
     4141 cggtgtcgtg gtgggcaccg atcatgcagc agaagccatt accggattct tcactaaata
     4201 tggtgacggc ggtacggata ttaatccgct gtatcgtctc aacaaacgcc agggtaaaca
     4261 gttactgacg gcattaggtt gcccggaaca cctttataag aaagccccaa ctgccgatct
     4321 tgaagatgat cgcccttctc tgccagatga agtggcactc ggcgtgacct atgacaatat
     4381 cgacgactat ctggaaggga aaaacctacc tgaacaggtc gccagaacaa tagagaactg
     4441 gtatctgaaa accgaacata aacgccgtcc gccaattaac gttttcgatg atttctggaa
     4501 aaagtaataa tttgcaccta ttggattgtg cgaaaaagag taatttgttc acgccggatg
     4561 cggcgtgaac gccttattct acctacaaaa ctatgcaaat tcaatacatt gcaggagtcg
     4621 aataggcctg acaggcgtag cacgtcagac ggtgtaacct ttgtcatcaa cccgcctctt
     4681 ttttaatcgc ctcccgcctg ttacactgga tagataacca gcattcggag tcaacagtgg
     4741 tacggcgttt aacttctccg cggctcgaat ttgaagctgc ggcaatttat gaatatcccg
     4801 aacatttaca ttcattcctt aatgacttac ccacccgacc aggggtgtat ctgtttcatg
     4861 gcgaaagtga caccatgccg ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc
     4921 tttctcattt acgcaccccg gatgaagccg ccatgctacg gcaatcccgg cgaattagct
     4981 ggatctgcac cgcaggcgaa atcggcgctc tgctccttga agcgcgatta atcaaagaac
     5041 aacagccgct gtttaataaa cggttgcgcc gcaatcgcca gctctgtgcc ctgcaattaa
     5101 atgaaaagcg cgtcgatgtg gtgtatgcca aagaggtgga tttttcacga gcccccaacc
     5161 tgtttggcct gtttgccaat aggcgcgcag ctttgcaagc attgcagagc atcgctgatg
     5221 aacaaaaact ttgttatggc ctgttgggac tggaaccgtt aagtcgcggt cgtgcatgtt
     5281 ttcgttcagc gctaaaacgt tgcgccggag catgctgcgg taaagagagc catgaggaac
     5341 atgcgctacg cttgcgccag tctctggagc gtttgcgagt ggtgtgttgg ccttggcaag
     5401 gtgcggtggc actgaaagaa cagcacccgg aaatgactca atatcatatt attcaaaact
     5461 ggctgtggct gggggcggtt aattcgctgg aagaagcgac aacgttaatt cggacacccg
     5521 ccgggtttga tcacgacggt tataaaattc tttgtaagcc gctgctttcc ggtaactatg
     5581 aaattactga acttgatccg gcgaatgacc agcgagccag ttgatttcac tgaacaacag
     5641 tttaccttgc ggttgtaaaa gccgcagggt gtgcctgcca tcgaaccagc acacgccctg
     5701 gtcggtagtg agcagtttgt cgcccagttg ccacgccccg ttaatcacaa acaccacccc
     5761 gccgcgtgag ccaaaggtag taaacgtacg ttcggcaatt ctgactttcg ccttacagac
     5821 atccaggcgc gtcataatat tgaaatccat cgacatctgc cctgccgtca gtttcgcttt
     5881 taccacctgg tccgccgtaa aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg
     5941 gtctgcgctt tcaaggagca tctcgccgcc ttccagcaac gtcactatcc tttccatgcc
     6001 gggaaacaaa gaaaactccc cattcgccgc gatggatgca atgctggcac gccagtaaaa
     6061 atcacgtttt gccggtggga aagtgcaaat ttcgcgcgtt tcaccggcag cgtttcgcca
     6121 cagattcacc gacattttac gcatatcaaa gtattccata ctcgctcccg gcccgttctt
     6181 ttcattgtta tcgttcaggt aaccgtggat ggttatcggc aactcgggtg atttacgtta
     6241 gtggtgatca ggcttttttt acaactgttt gagaagagaa aagaaaaccg ccgatcctgt
     6301 ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaag
     6361 attattcagc agttgcaggc attttacctt ttgccgctgg acgttctgtc agacgcttct
     6421 caaaattagc attaaattgc tttttctgtt ccggcgtcag gatgttgtaa attttgttct
     6481 gggtttccat gtgcgccagc atgttagctt tgcgctgttc ttccattttt gcgatctgcg
     6541 cttcagcttt tgctttatcg aaggtatcgc tggcaatgat gtcatgcatt gcgcggcgtt
     6601 cttccagcgg cggacgtttc atctggtcac gctggccttt catgatttcg tggatctgct
     6661 gtttctgcgc gtcggtcagg ttcaggtctt tgaacatcat gtcctgatgc ggaccgaact
     6721 tgcctttgtg gtgcatcatc ggcttcgcgt cagccggtgc tgcggtagtg gtgtctgcgg
     6781 catgggccag gttagccgcg ccaagagcca gggtagaggc aacaaacagt gcagttaatt
     6841 tacgcatatt ctatatcctt cctttcagtt atttattacg gctttcttaa gtagcgtgcc
     6901 gtgttgacga gattaacttt actgacttta gcgtcaatta atcagagcaa cggtaaaaca
     6961 atgaaagtgt aaaaaacact tttgcgccaa ttatggagaa aaaaagaaaa tttgatggag
     7021 agtgatgaga gaatattaca acacgatgat tttgcagaga ttatgaagaa ctataccgga
     7081 tgactggtga taaataaagc aaataaccag gattaatctg tattaattta taagaaagca
     7141 acttaatacc cgcagaatga tttctgcggg taagtattag cttatttttt cgagcattaa
     7201 tcccgcgcgt aatcccaacg ctaccaacgg attagggaat aacacatact ctacatcatg
     7261 ggttacggta aaacgttcct ctccgtcctg cgccagcaat gttcctttct caaacggcat
     7321 aaaattcagc gtgtcacttg ccatatgcat ttcgaaggac ggcgagtggc gagtaatttg
     7381 cgaaaccacc cgataacgga gcggcggtgt tctcacgata ccgacactct caccagatag
     7441 cagcgcagca attgcgctgg cagttactgc aaactggcga agatcgtttt gcccaaaggg
     7501 caacgctttg ccaagttcca acgtacaggc cagcgcgcca aaatgtctga cgctgaaatg
     7561 ggtaaacgta ccaccaggct cctgatggaa caccagcgcc tccagccccg ccgcacccag
     7621 ccacgtcaga aatttctcgt cccagggaat gtcgcgttgc ggtaatacac cgaactgcgg
     7681 atgcaaggag ccacgaattg cggtatgtag atcaaggtgc caacgcacag attctttgcc
     7741 ctggtcataa aaatcttcca ggcactgttc cagttcgcgc gcccgacagg tttctccgct
     7801 ttcagcaaat agctgccaac gaccgccaaa cattcggttc atatcgctat ggcaataacg
     7861 tttcccttgc ttcagcgcag gaggattccc gaggatcacc agcaaccgcc aacgtaacgg
     7921 gatctcgccg tgagatatcg cgccaagcaa cgcgtccagc atttccacag gtgccgtctc
     7981 attaccgtgg atccccgcag aaatcaccag cgcgccttgc ggtggcgtta atggcgtcag
     8041 ttccagcaca ccatcgccca gccagcgcca gcgaacgccg ttgatttctc gctcggtgat
     8101 aaccggtttt ttacccgtta aggtcagagc aagaaaatta tccattgccc ctccctcgcg
     8161 ctggaacgga taaaccgaac cgagattcag taattggctc aatacatcca gtgcttcccg
     8221 cccttcgcgc agcaattgcg ggtcggccag atcggcagca gtaagacgat cgcggtagta
     8281 acgatccacc cagtcattga gcgcattaaa caaggtatcg ttcatcatca ccgccggatt
     8341 caccgcccgg cgttcttctt ctgtcaatac cacccgcaac cgcaggcacg ccgggccgcc
     8401 gccattcgcc atgctttcac gtaagtcaaa gacttttagt tcgctaatcg ggttgtcagc
     8461 ggcaaggagt tcattgagat aaccccatac tccggcgtgt tcccgacact cctgaggcag
     8521 caccaacatc atggaaccat catcgcggct caacagttgg ctgttaaaca gataggtaga
     8581 caccgcatcc gacacggaaa cctgagttgc cggaacttct atcgccataa aaccgttcac
     8641 ccgcgtgcca gtaactgtga ctggcgagcg aacgcctgtt ggtggcaaaa cagcacctgg
     8701 cggttactca cggcaatcac gtcattatga aaaacgctct ggtcgataac gtccgggttt
     8761 tgctgggcga aaatcacctg ttggggattc acctgattaa gccttgccac cgcctcgctg
     8821 gcttcgcgag tctgtcgcgc cggataacgg gaaggccggg tatcattgcc ttcttctcgc
     8881 ccgtagacaa aaagtagcat acccggttca ccgtaatgac cgccgagacg attgtggttt
     8941 gccgccccct catcaccgag caacgctacc tgcggcaacg ccgaatggac gctaaatttc
     9001 tcttcgtcct taaaaatcgc ttttaacagc gattcagtga cgggcgcttc cagcgaacgg
     9061 tgaaatttat tgttcaggtt ggcaacggtg agatgcactt tgccatccag cgtatcggca
     9121 gatggcgcga tcgttgccgc attggctacc cacattggcg aagcggaact gacgctggaa
     9181 agccagtgcg gtgcctggcg tgcaactttt tccagtacct gctcatcgct accactgaat
     9241 cccaactgac gcagcaccgg aataaacgga cgctcgtgcg gcggaatcac ggcctggggg
     9301 aatcccgcat cggcaagggc tttcattttc agtaagccct gttttgccgc cagtcgcggg
     9361 ttagacacct gaaaacggtg acgggtagag gcttcattac caaacgacag gcccgcgtaa
     9421 tgatgcgtca gccctaccag cccgtcgaaa ttaacttccc aggcgttcat cgcaccacct
     9481 catcggaaaa atccagcccg gggttaagcg tggcgggcaa tgttaacgag tccgactcca
     9541 ggctcgccat aggccatgcg cagtaatctg cggcatacca ggcgctgggg cgatggttac
     9601 cggaagcacc aatgccgccg aatggggcgg tactggcagc accagtaagc ggtttgttcc
     9661 agttaacaat ccccgcccgc gcctccagca acagttgatc gaatttttcc cgctcggggg
     9721 aaaccagacc gcaaaagagt ccgaagcgag tgttgttcgc catccgaatc gcctcatcaa
     9781 aagtatcata acgccagacg cgcaaaaacg gcccgaacac ctcttcatcc ggcacaccgc
     9841 caacgcccgt catttcaatg atccctggtg tcagcaacga tgtccctgct tgtaataagc
     9901 gcggcgcaag cagggttcgt ccgcccatcg cttccagttg ctgccaggca gtaaccacct
     9961 gctgtgcggc ctgttcagaa atcagcccgc caataaacgg ctgcggttcg tcatcccagt
    10021 tgcccggcgt taatcgctgg ctgacggcaa ccagactagc aagaaacgca tcgccctgcg
    10081 ctccgctttt gagcaataaa cggcgggcgc aggtgcagcg ttgcccggct gtgacaaacg
    10141 ccgactgaat ggtcagatgg acagccgcgt cgatatccgc cacctcatcg ataattagcg
    10201 gattattacc gcccatctca agggcgagaa ttttctccgg ctgaccggag agctggcgat
    10261 gcagctggta gccggtattg gcgctaccgg taaacagcaa accgtcgaga tcctccagcg
    10321 cactcagcgc ctgacccgtt tcacgcccgc cctgcaccag gttcagcacg cccggcggca
    10381 agccagcctg ctgccataaa cgcattaccg cttcgccact ccacggtgtc agttcgctgg
    10441 gtttaaagat aatggtgtta cctgccagca atgccggaac gatatgtccg ttcggcaaat
    10501 gaccagggaa attatacggc ccaaacaccg ccagcacgcc gtgcgggcga tgtcgcaggc
    10561 tcgccgcacc gtccggcatt tcactacgct gctcgccggt acgaacgtga tacgccttaa
    10621 ttgatatcgc gattttattg atcatcgccg tcacttcggt tgccgcttcc cagcgcggct
    10681 tacccgtttc tctggcaata atcgcggtta attcggcttt attgctttcc agcaatccgg
    10741 caaagcgttc gacaacgacc tgacgttcag ccaatgagag ccgcgcccag cgcggaaacg
    10801 ccgcacgggc tgcccgacaa gcctgttcga cctgagcggc atcggcatca ttgccttgcc
    10861 ataacacctc gcccgatacc ggattacgct tcacacgcga tgcgccctgg cccgttatcc
    10921 agtcaccgtt aatccataaa gtcatgctgt tttctcctct gcgcacaggc gcaccagacg
    10981 aacgcgatcc cctgcgtggc atttgagggc atccagttgt gcggcggtta aaatcaaacg
    11041 ctcggttgcc ggatcggtac gcgccagcac cacgcggaaa tggtgataat tttcattggc
    11101 gaccaggcag gccggaaaat cgccctgcgc aggctgccct tctgccactt ccaccagccg
    11161 acttttacgg atggcgcgca cgcggtcgat gtcacactca agcgttggcc cgccgtcgaa
    11221 gatgtcgata tagttgcggt aacgaaaacc ttctttctcc agcaccgcgc gggcaggcgc
    11281 ggtttgcgga tgtacctgac cgatgacgtc ctgggcttcc tgggataaaa agtgggtata
    11341 gatcggatgt ttcggcatca gttctgcaat aaatgccttt tgcccggtgc cgcagagaaa
    11401 atcggcgcgg ctaaaatcca tcgaaaagaa gcgtttaccg aggctttgcc agaacggtga
    11461 atagccgtgt tcgtcaatca ccccgcgcat ttcagcaacc actttgtcat taaacttgtc
    11521 gcgaaaagcc gccataaaca taaagcgcga tttcgacagc aaatagccgt tgccctcttt
    11581 gcgccagtcc gggtcgagaa acagcgtgca cagctcgctg ctgccggtgt gatcgttact
    11641 aagaaacagc gtcggcaatg cgttatagac attcagctct tttgaggcgt gaaccaacgt
    11701 gccgacgcga tagttgtacc agggatcgtt cagcccaacc gccacctcaa tagcgcaaat
    11761 ccccgccacg gtgcctgtct cgctatcttc cagcacgaac acatagccct gctcgctttt
    11821 gggcagttca ccttgccagg ttttgattgc cctttcgata cgcgccgaaa gcgtggcttc
    11881 attggcggga agcgacgtca ggccgccgcc cgttttgctg gcaagctgca tcagcgccga
    11941 gacatctgag cgctcaacgg gacggatgac catcatgatg aacctcggct aacaaagtgt
    12001 tcgcaagcag ctgcaaagcg atccagtccg gtcgtcacct cttcttcgct gacattgagc
    12061 gcaggcgcaa aacgcaccac gttgccaccc gcaatcagta ccatcacgcc tgctttcgcc
    12121 gcttccagag agatctgttt cgcttgcccg gcgtaatcgg cattcagtac acagccaatc
    12181 aacaaaccta agccgcgaac ttcactgaac aaaccatagc ggtgattaat agtattaaga
    12241 cgctcaacaa accagtcgtg acgctgttta acgccattaa gcatctctgg tgtgttgatg
    12301 agctccagca ctttgcctgc taccgccgag gccagcggat taccgccata ggtggtgcca
    12361 tgagtgccaa cggtcatcac gcgggcgcac tcttcggttg ccaacaacgc accgaccggg
    12421 aaaccgccgc ccagcgcttt ggcggtggtt aacagatcgg gcgtcacgcc gtagtgcata
    12481 taggcataca gctcaccagt gcgcccgacg ccggtttgta cctcatcaaa aatcagcaag
    12541 gcattgtggc ggtcacacaa ttcacgcaga ccttgtaaaa acgcgttgct ggctggcacc
    12601 acaccgcctt cgccctagat gggctcgaca atcaccgcac aggtggcgtc gtcaatcagc
    12661 gcgctggcag agttaatatc gttatatgca gcatgacgaa tatccgccgg cagtggcgca
    12721 aaatcctgtg aataggctgg ctgcccaccc gcactgacag taaacagcgt gcgaccatga
    12781 aacgcatttt tgaacgccac gatgccactc ttatggctgc cgtagcggtc gtgagcgaat
    12841 ttacgcgcca gttttagcgc cgcttcgttg gcttccgcac cggagttaca aaagaagacg
    12901 cgatcggcaa acgtggcgtc gatcaatttt ttcgccagtc gcagtaccgg ctcgttggtg
    12961 taaccgttgc cggtatgcca gaacttactc gcctgttcgt tcagcgcttc acgcagttcc
    13021 ggatgcgcat ggcccagcgc gttcaccgca atgccacccg cgaagtcgat atactctttc
    13081 ccctgctgat cccacaagcg cgaaccttcg ccacgtaccg gtataaaggg tgccggagcg
    13141 taaacaggta tcatccattc atcaaagttt tcacgcgtaa ttggctgaga catagcgacc
    13201 tctacagtaa ataattcgtt atttgtatgt taacaataag taatgtttgc gctgtaaatg
    13261 tagattgcag ggttcgtgcc agccagtgat aaaagtgcat aaacggcgga ggctaactgg
    13321 aaatcaagga gttataacca aaccatatgc atttaaagtg catataaagt gaatacgttt
    13381 gcgatgtggg tgaataaaaa gaataaaaaa cgcaatgtta tgcagaagta aaatataatt
    13441 ctagaattgt gatcattgac gaaatttact ggaaattact gcgccattct gacgcagcgc
    13501 gcaccaaaag cgggcatttt ttgcgccatc gttgacatca ttaacaacca tcgatcaaat
    13561 cacttaacaa caggcggtaa gcaacgcgaa attctgctac catccacgca ctctttatct
    13621 gaataaatgg cagcgactat gaaatttgtc tcttttaata tcaacggcct gcgcgccaga
    13681 cctcaccagc ttgaagccat cgtcgaaaag caccaaccgg atgtgattgg cctgcaggag
    13741 acaaaagttc atgacgatat gtttccgctc gaagaggtgg cgaagctcgg ctacaacgtg
    13801 ttttatcacg ggcagaaagg ccattatggc gtggcgctgc tgaccaaaga gacgccgatt
    13861 gccgtgcgtc gcggctttcc cggtgacgac gaagaggcgc agcggcggat tattatggcg
    13921 gaaatcccct cactgctggg taatgtcacc gtgatcaacg gttacttccc gcagggtgaa
    13981 agccgcgacc atccgataaa attcccggca aaagcgcagt tttatcagaa tctgcaaaac
    14041 tacctggaaa ccgaactcaa acgtgataat ccggtactga ttatgggcga tatgaatatc
    14101 agccctacag atctggatat cggcattggc gaagaaaacc gtaagcgctg gctgcgtacc
    14161 ggtaaatgct ctttcctgcc ggaagagcgc gaatggatgg acaggctgat gagctggggg
    14221 gtggtcgata ccttccgcca tgctaatccg caaacagcag atcgtttctc atggtttgat
    14281 taccgctcaa aaggttttga cgataaccgt ggtctgcgca tcgacctgct gctcgccagc
    14341 caaccgctgg cagaatgttg cgtagaaacc ggcatcgact atgaaattcg cagcatggaa
    14401 aaaccgtccg atcacgcccc cgtctgggcg accttccgcc gctaatttag cagctctcct
    14461 ggctcaaact gggtcaggag aattaacctt gagaaaaatc aacaaactgt cagtaatgat
    14521 ttgttgcctg ccgtcctttg ttataccgtc actgcgtttt tagttgtctg accacttctc
    14581 tattatcaag tttgatatag gaaactccac gatgaacgct gagcgtaact ttctgtttgc
    14641 ctgtcttatt tttgcgctgg tcatttacgc tatccacgct ttcggtttat tcgatctgct
    14701 caccgattta ccccacttac agacactcat tcgccagagc ggattgttcg gttatagcct
    14761 ctatattctg ttattcatca atgcccccct ctttctgtta ccaggaagca tactggtgat
    14821 cgctggagga atagtttttg gcccgctctt agggacacaa ctctcattaa ttgccgcaac
    14881 gctggcctcc tcgtgctcat tcctgctggc gcgctggctg ggacgtgatt tactgctgaa
    14941 atacgtgggc catagccata ccttccaggc cattgaaaaa ggcattgcgc gtaacggtat
    15001 tgattttctt attctgaccc gcttaatccc gttgtttcct tacaatattc aaaattacgc
    15061 ttacggattc accaccatcg ccttctggcc ttataccctt atttcggcac tcacgaccct
    15121 gcccggtatt gttatttata ccgtgatggc aagcgatctc gccaatgaag gcattacgct
    15181 gcgctttatt ttacaactct gtctggcggg cctggcgctg tttattctca tccagctcgc
    15241 aaaactctac gcccgacaca aacatgtgga tctgtctgct tcgcgccgca gcccacttac
    15301 tcacccaaaa aatgaaggat agaacgatgt cgcaacatta ttcagtgtca tggaaaaaag
    15361 gactggctgc actctgctta ctggctgttg ccgggcttag cggctgcgat caaaaagaga
    15421 atgcggcggc aaaagtggaa tacgacggac tttcgaacag ccaaccgctg cgcgtcgatg
    15481 ccaataacca tacggtaacc atgctggtgc aaattaatgg tcgtttcctc accgacgaca
    15541 ctcgtcacgg tattgtgttt aaagatggct ccaacggaca taaatcgctg tttatggctt
    15601 atgcgccccc gaaagcattt tatgaagccc tgaaagaggc aggtggtacg ccgggcgaaa
    15661 acatgacgat ggataataaa gaaacgactc atgtcacagg cagcaaactg gatatttcgg
    15721 tcaactggca aggggcggca aaagcgtatt ccttcgatga agtgattgtt gacagtaatg
    15781 gcaagaaact ggacatgcgc tttggcggta atttaacggc agcagaagag aagaaaacgg
    15841 gttgcctggt gtgtctggat agctgcccgg tcggcatcgt cagcaatgca acatacactt
    15901 atggtgcggt ggaaaaacac ggcgaagtga aattcaaagg caatgcctca gttctcccgg
    15961 cggataacac gctggcaacg gttaccttta aaatcaccga ataaagccaa gataaaggat
    16021 gatgaagata caatcgcgta aaatctggta ctaccgtata accctcatca tcctgttgtt
    16081 cgctatgctg ctcgcatggg cgctgcttcc cggcgtccat gagtttatca atcgcagcgt
    16141 tgcggcgttt gccgccgtgg accaacaggg tatagaacgc tttattcagt cttacggcgc
    16201 actggcggca gttgtctcgt tcttgttgat gattttacag gccattgctg caccgctacc
    16261 tgcgtttttg atcacctttg ccaatgcgtc gctgtttggc gcgttctggg gcggcttgct
    16321 gtcgtggacc agttcgatgg ccggcgcggc gctgtgcttt tttatcgcca gagtgatggg
    16381 ccgcgaagtg gtggaaaaat taaccggcaa aaccgtgctt gacagtatgg acggcttttt
    16441 cactcgctac ggcaaacaca ccatcctggt ctgtcggtta ttgccttttg tccctttcga
    16501 tccaatcagc tatgctgccg gtctgacttc aatacgtttt cgctcgtttt ttatcgccac
    16561 cgggcttggt caattaccgg cgactattgt ttattcctgg gcaggcagca tgttaacagg
    16621 cggtactttc tggtttgtca ccgaactgtt tattctgttt gccctgaccg tggtgatttt
    16681 tatggcgaag aaaatatggc ttgagcgcca gaagaggaat gactgatggg tttaccgccg
    16741 cttagcaaaa ttcctttcat tttacgtccg caggcgtggc tgcatcgtcg ccattacggc
    16801 gaggtgctaa gcccgattcg ctggtggggg cggatcccgt ttatctttta tttggtgtca
    16861 atgtttgttg ggtggctgga gcgcaaacgc tcaccgctcg atccggtagt gcgatcgctt
    16921 gtcagcgcgc gcattgcgca aatgtgcctg tgtgagtttt gcgtggatat caccagtatg
    16981 aaagtcgccg agcgtaccgg cagcaccgat aaactgctgg cagtggctga ctggcggcaa
    17041 agcccgctct ttagcgatga agaacggctg gcgctggagt acgccgaagc cgcaagcgtc
    17101 acaccgccaa cagtcgatga tgccctgcgt acccgactgg ctgcgcattt tgacgctcag
    17161 gcgctcaccg aactgacggc attgatcggc ctgcaaaacc tgtcggctcg ttttaattct
    17221 gccatggaca ttcccgctca ggggctgtgc cgtattccgg aaaaacgttc ttaaggaggg
    17281 aagatgcgcc attgtgggtg gttgctggga ttgttatcgc tgttttcttt ggcaacacat
    17341 gccagtgact ggcaggaaat taaaaatgag gccaaagggc aaaccgtctg gtttaacgcc
    17401 tggggcggcg ataccgcaat taaccgctat ctcgactggg ttagcggcga gatgaaaacc
    17461 cattacgcta taaacctgaa gattgttcgt ctcgcggatg ccgcagacgc ggtgaagcgc
    17521 attcagaccg aagctgctgc cggtcgtaaa acgggcggct cggtggatct gctctgggtg
    17581 aacggcgaaa acttccgcac cttaaaagag taatgccagt cagttaagca actgactggc
    17641 att
//
LOCUS       DAZSIO010000079        17378 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZSIO010000079 DAZSIO010000000
VERSION     DAZSIO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.079"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            176..778
                     /gene="ais"
                     /locus_tag="ACHX5T_002386"
     CDS             176..778
                     /gene="ais"
                     /locus_tag="ACHX5T_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR8467555.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(817..1242)
                     /gene="nudI"
                     /locus_tag="ACHX5T_002387"
     CDS             complement(817..1242)
                     /gene="nudI"
                     /locus_tag="ACHX5T_002387"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR8467556.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1521..2063
                     /locus_tag="ACHX5T_002388"
     CDS             1521..2063
                     /locus_tag="ACHX5T_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR8467557.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            2150..3352
                     /locus_tag="ACHX5T_002389"
     CDS             2150..3352
                     /locus_tag="ACHX5T_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR8467558.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            3416..3877
                     /locus_tag="ACHX5T_002390"
                     /pseudo
     CDS             3416..3877
                     /locus_tag="ACHX5T_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(3918..4868)
                     /locus_tag="ACHX5T_002391"
     CDS             complement(3918..4868)
                     /locus_tag="ACHX5T_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR8467559.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(5061..6251)
                     /gene="glpC"
                     /locus_tag="ACHX5T_002392"
     CDS             complement(5061..6251)
                     /gene="glpC"
                     /locus_tag="ACHX5T_002392"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR8467560.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(6248..7507)
                     /gene="glpB"
                     /locus_tag="ACHX5T_002393"
     CDS             complement(6248..7507)
                     /gene="glpB"
                     /locus_tag="ACHX5T_002393"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR8467561.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(7497..9125)
                     /gene="glpA"
                     /locus_tag="ACHX5T_002394"
     CDS             complement(7497..9125)
                     /gene="glpA"
                     /locus_tag="ACHX5T_002394"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR8467562.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9398..10756
                     /gene="glpT"
                     /locus_tag="ACHX5T_002395"
     CDS             9398..10756
                     /gene="glpT"
                     /locus_tag="ACHX5T_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR8467563.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            10761..11837
                     /gene="glpQ"
                     /locus_tag="ACHX5T_002396"
     CDS             10761..11837
                     /gene="glpQ"
                     /locus_tag="ACHX5T_002396"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR8467564.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(11879..12121)
                     /locus_tag="ACHX5T_002397"
                     /pseudo
     CDS             complement(11879..12121)
                     /locus_tag="ACHX5T_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            12116..12220
                     /locus_tag="ACHX5T_002398"
                     /pseudo
     CDS             12116..12220
                     /locus_tag="ACHX5T_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12300..12950
                     /gene="inaA"
                     /locus_tag="ACHX5T_002399"
     CDS             12300..12950
                     /gene="inaA"
                     /locus_tag="ACHX5T_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFR8467565.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(13004..13258)
                     /gene="yfaE"
                     /locus_tag="ACHX5T_002400"
     CDS             complement(13004..13258)
                     /gene="yfaE"
                     /locus_tag="ACHX5T_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR8467566.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(13258..14388)
                     /gene="nrdB"
                     /locus_tag="ACHX5T_002401"
     CDS             complement(13258..14388)
                     /gene="nrdB"
                     /locus_tag="ACHX5T_002401"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR8467567.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(14622..16907)
                     /gene="nrdA"
                     /locus_tag="ACHX5T_002402"
     CDS             complement(14622..16907)
                     /gene="nrdA"
                     /locus_tag="ACHX5T_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR8467568.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPNIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            17035..17178
                     /gene="ypaB"
                     /locus_tag="ACHX5T_002403"
     CDS             17035..17178
                     /gene="ypaB"
                     /locus_tag="ACHX5T_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR8467569.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         4097 a         4230 c         4641 g         4410 t
ORIGIN      
        1 aaatattgaa atttttatat tttttcttat ttaggctttg catttggcaa aaatttgagg
       61 cattttgccg acatcgtagg atttttaata ttacaccaac tgctaattat cgccagaaat
      121 gtcgctcaat ttcacggtaa ttgtctggtt gcgcttgtct ataggtggag tttacgtgtt
      181 agctttttgc cgctcatcat tgaagtcaaa aaaatatttc atcattttac tggcgctcgc
      241 tgcaattgcc ggactgggta ctcatgccgc ctggagtagc aatggtttgc cacgtatcga
      301 caataaaaca ttggccagac tggcacagca gcacccggtt gtcgttttgt ttcgccatgc
      361 tgaacgttgc gatcgttcaa ccaatcaatg cttgtcagat aaaacaggta ttacggttaa
      421 aggtacccag gatgcccgtg aactgggcaa cgcttttagc gctgacatct ctgatttcga
      481 tctttattcc agtaataccg tccggaccat tcagtcggcg acctggtttt ccgcgggtaa
      541 aaagctgacg gtagataaac gacttcttca gtgcggtaat gagatttata gtgcaattaa
      601 ggacttacaa agcaaagcgc ctgataaaaa tatcgttatt ttcacccata atcattgcct
      661 gacatatatt gctaaaaata agcgtgatgc gatatttaaa cctgattatc tggatggttt
      721 agtcatgcat gtggaaaaag gcaaagttta tctggatggg gaattcgtta accactaaat
      781 tttcaatctg acagccagtg atggctgtca tcattgttaa agaagacctt tcaaacgtaa
      841 cgtttttcgg gtggcaacat ttaaatcata atgcaccaga tcttcaggtt ttacccacgc
      901 gtagtcctga aactcttcgt ttattttcac ttctcggttg gcagaaacgc agtcaaaaat
      961 caggtaaatc atataaatct cttccttgcg accatctgca tatgtcttgg tgcgaatatc
     1021 atcgctgaag gtccacggcg tgatttctgt caaaagcagc tgttctccca gttcttcgcg
     1081 aatttcgcgg cgtagtgcct cttcaattcg ttcgccagac tccacgccgc cacccgaaag
     1141 cgcccattga ccggggaaaa cgccgcgatc gtcggccatt ttgcacagca aataagcacc
     1201 atcattttga atcaaagggc atacaatagt ccgttgtcgc acaggggtat tccttataaa
     1261 taaaaagaaa ccatgagaat acggtaactc agaaatatgt gaaagtgaca gtaatctctt
     1321 ttgcaatgat aactgcactt attattattt ttgtgtgtat cggtaaagtg tgtttatttt
     1381 ttcggcgttt aattatttaa gaagtattta gttacaataa gtgactagat cccttttttc
     1441 gctcattgaa tcctctgtgc caggcagtac aatcccgcag ctttgggaat tttgattaat
     1501 tgcttgtggg aaaggggata atgaaaaaaa ttgcgctagc aggtctggcc ggaatgctgt
     1561 tggtttctgc atcggtcaat gcaatgagca tcagcggcca ggcgggtaaa gaatacacca
     1621 atattggtgt cggttttggg actgaatcga cgggcctggc tttaagcggt aactggacac
     1681 ataacgacga cgacggtgac gtcgcgggcg tggggctggg gttgaatctg cctctcgggc
     1741 cgttaatggc gaccgttggc ggaaaaggcg tgtacaccaa cccgaattac ggcgatgaag
     1801 gttatgccgc agcggtagga ggtggtttgc agtggaaaat tggcaacagc ttccgtttgt
     1861 ttggcgagta ttactactct ccggattcgc tctccagcgg tattcaaagt tatgaggaag
     1921 cgaatgctgg cgcgcgttac accattatgc gtccagtcag tattgaggcg ggttatcgct
     1981 acctgaatct gtcgggtaaa gacggtaacc gcgacaacgc tgtggctgac ggcctgtatg
     2041 ttggggttaa cgccagtttc tgatcccttc cggcgcggca ttcagtttct tactacgcct
     2101 tatctccctt tgcgttatag tgattcgata acaaaagcag gagataacca tgttaaaagt
     2161 ggaaatgcta tccaccgggg atgaagtgtt atacgggcaa atcgttgaca ctaacgctgc
     2221 ctggctggcc gattttttct ttcatcaggg gctgccatta tctcgccgca atacggtggg
     2281 ggataacctt gatgacttag tcaccattct tcgcgaacgt agtcagcacg ccgatgtgct
     2341 gatcgttaac ggcgggctgg gaccgaccag cgatgattta agcgcactcg ccgctgcgac
     2401 agcaaaaggt gaaggcctgg tgctgcatga agcctggctc aaagagatgg aacgctattt
     2461 ccacgaacgt ggacgagtaa tggcaccgag caaccgtaaa caagcggagc tgcctgccag
     2521 tgctgaattt atcaataacc cggtaggcac cgcctgtggt tttgccgtgc agcttaatcg
     2581 ttgcctgatg ttctttactc ccggcgtacc gtcagaattt aaggtgatgg tcgagcacga
     2641 aatcctgccg cgcctgcgcg agcgtttttc tttaccacag ccgccggttt gtctgcgttt
     2701 aactaccttt ggtcgttcgg aaagcgatct ggcacaaagc ctggacactc tacaactgcc
     2761 gccgggcgta acaatgggct atcgctcctc aatgccgatc atcgaactga aactcaccgg
     2821 accggcaagc gagcaacagg cgatggaaaa actgtggctg gacgttaaac gtgttgccgg
     2881 acagagcgtg attttcgaag gcaccgaagg actgcccgcg cagatcagtc gcgaattgca
     2941 aaaccgccag ttcagcctga cgttgagcga gcaattcacc ggtggtttat tggctttgca
     3001 actttctcgc gcaggtgctc cattgctggc gtgtgaagtg attccttcac aggaagaaac
     3061 cctggcgcaa actgcgcact ggattacaga acggcgggcc aaccattttg ccgggctggc
     3121 actggctgtt tcgggtttcg agaacgagca tctcaacttt gcgctagcca cgccagacgg
     3181 cactttcgct ctgcgtgtgc gtttcagcac tacacgctac agcctggcta tccgtcagga
     3241 agtgtgcgca atgatggcac tgaatatgct acgccgttgg ttaaacggcc aggacatcgc
     3301 tagtgagcat ggctggattg aggttgttga gtccatgact ttatccgtct gatttcgcca
     3361 ctctgtgccg gtttactgca ccggcacatt ccccctccgg caaaatttgt ccaaccctcc
     3421 ctacggtgag cgtggtgttg gggccagtgt ggcacgggct gcgcgctggg gacgcattga
     3481 gaattacatg gcgcaagtca acgattcgct ttgtctgtta gtgcaggtgg aaagtaaaac
     3541 ggcactggat aacctggacg agatcctcga cgtcgaaggg attgatggcg tgtttattgg
     3601 accagcggat ctttctgcgt cgttgggcta cccggataac gccgggcacc cggaagtgca
     3661 gcgaattatt gaaaccagca ttcggcggat ccgtgctgcg ggtaaagcgg ctggttttct
     3721 ggctgtggct cctgatatgg cgcagcaatg cctggcgtgg ggagcgaact ttgtcgctgt
     3781 tggcgttgac acgatgctct acagcgatgc cctggatcaa cgactggcga tgtttaaatc
     3841 aggcaaaaat gggccacgca taaaaggtag ttattgatat caaaggccca tggggatcgg
     3901 ctgtgggcct gtgttaatta gtggttattc gctgccagat cggcttcgct tagctgggtg
     3961 gccgcgagca cttggtcacg atcaatgccc tgttccagca tgcgcaaagc aattttaatg
     4021 gcttgttcat gcataccttc ctgcaaacct tctaatttac cttgctgcaa acctactaat
     4081 ttaccttctt gccagccaat ttgatgtcct tcctgccgta gccgttcagc aatagtcatt
     4141 aatctctcct tttgtagtgg tgaacgttcg gcaatctcct gaataaaacg ggtgaaacgg
     4201 gaggtatcgc cgcattgcag cagataatta aacagtgttt gtagctggct gtcattagtg
     4261 aagcctctaa ccaaaagcgt ggtgatcctg tcgaccatgc cgattaaatc gtggtcgcga
     4321 atatgctttt gaatcagttc cagcagagct atacgccgat gttgcatgat ctcatcgtca
     4381 ggtacgatgg tgatatccac caacggaaaa gcttcggtgt acaactgccg ggcaagttgc
     4441 ggatcgtcaa actcatccag ccagttaagt gagtacgggt agggcgatgt ttcgccatga
     4501 taaaacagca atggcaccac cagcggaact ctgtcatagc ctttatctag gtgactctgc
     4561 atggcggcag tggcatagcg cattggccga aaagccatat tcttttctgc agagctttga
     4621 tgttcaatca cgcagtagat atagccgtca ccgtcgctgg tttccacgga ccacaaaaca
     4681 tccgagtagt aagcgcgtaa acttttttca ataaaactag tgggttccag gcgtaaggtt
     4741 tgcaggttgc aaagcttgcg cagtggttct ggtaaatgta tttcgagaaa atcccgtgcg
     4801 gtttcgggtg tgaatataaa ggttttaaat accgcatcat gcggggagga ggttgttgat
     4861 tcggtcatgg ttgtccgtca cctgatattt atgatgacgc gacattacgg ttatcccagg
     4921 cctttgacat ctgccagttt cagcctttgc gagacgtttt cagaggaatt ttcactgcgt
     4981 tgcaacgcat acattgggca cggaatcgtg tacgcaaaaa tgcccggtaa ccacatgctt
     5041 accgggcatc agaagggagt ttaagccagc gcctgggcca gtagcgtaat cggatgttcg
     5101 cagcgaagac ttgtggacat ctcaatctgc catttacagg tttcgcagtc ggtgaccacc
     5161 agatctgcgc cgctttcttc tatctggcgg aacagtggtg cgccgatggc ttgtgaggtg
     5221 gggtagttct cttttttgaa accgtaagta cccgcaatac cgcagcactg ggaatccagc
     5281 accgttaact caagccccgg gatgttacgc aacagctcca gggtgtagag cgtccagccc
     5341 attttttcca tatggcacgg agtgtgataa accactttca gcggcagcgg tttcagcggt
     5401 aacgttttgc cttcgtccag cttgcgccac agccagcggg ttgccagttc gatatgatcg
     5461 cgcaagcctt tgttgtcgac attcagcact tccgggtatt cgtcgcgcag ggcaaatgta
     5521 caggttgagg aggtggcaat caccggaatg ccttttactc ccacagcttc gcggatcgac
     5581 tctacattcg taattgcctg tttgcgtgct ttatcggtaa agccgttggc gattagcggt
     5641 acgccgcagc atttttcttt gctgagcagt tgtacaccgg tacccattgc gttgagcact
     5701 ttaattaaat ctttacctaa ctgcggatgg ttgtagttaa cgaagcagcc gtgaaagaaa
     5761 gcgacctggt ctttatattg tgcttgctga gccgctatgc tgcgatacca gcgacggaac
     5821 gtgccgaagg agtatttcgg tagcgtgcga cgatgatcga tttttaacgc cgcatcaagc
     5881 agctgccgca ccggtttcag cgaggtagcg gtgttgacga ttggtgcgaa cggcgtggaa
     5941 acgctaccca tcaggtcggt atgactcaac acaaaattac gcagcgacgg gcgcgtggtg
     6001 tcatatttcg cccgcgcgcg ctggataata tcgccaatct tcacatcgga cgggcaggcg
     6061 acttcacaac gtttgcagtt gatgcaatat ttcagcgcct cgtcatacag tgcgccatct
     6121 ttcaaacgca gacgctcgcc atccggcccg gcttgttttg gccctggata accgggattc
     6181 acccggctca ccgggcaggc ggtggtgcag acggtgcact taatgcagtt ttcgaagctg
     6241 gtgtcgttca ttgttggcct cctgcgcgtt gggcaatctg ttgagcggca tgtaaagcac
     6301 tgacggcaca aacaccgccg ccgcatccct gggcgatggg atcaaatccg cccagcaccg
     6361 aaccgatggc aaacaggttc tcaatggttt gccctgcctg cgacgggcgt agcgtctcat
     6421 cagtggttac accgaactgc tgccacggtt gcggcgcaaa aaaatcgccc ttataccatt
     6481 caccccgcgt ggcggtttgt agcacatcaa ggccgagaat cggctcgcga atgccgttac
     6541 gttccgctac tagtccgcca ctaaagaaac tgccgctggc gagaaccgcg aaacgtggac
     6601 gtagcggaat atcggcgtga ttgcgggtcc agatttcatt cactacgcca tttttacagg
     6661 tcactttttt cacttcatcg cccggcatcc acactccacc ttggcgcaca aactggcgct
     6721 gtaactggtt ttgcagacga atgcccagca cggaaggcgg cagcgttggc aaaagcatca
     6781 gtgaacaagg tagtttttca ttcaaccaac gccacagttt gtcatcggcc aggccgaagc
     6841 aggcgggcat caggatcatt tcgcaggtat tggcgacagg aataagcgca tcaagtaaca
     6901 gcggccagtt ttcttcatta tcaaggaaac gggcgatatt caccgcgcga aattcggtgg
     6961 cgttattgcg caggacatcc agttccggca gctctatttc tgcggtttca accgaaagat
     7021 ccagttcgcg caacgaagcc gccgcaagat gcgcctggaa atccatcagc ccgcttatcc
     7081 ccactacaca tatcttcttc gcgggtagcg gccagacggg aacttctggc gaacttaacc
     7141 aggttgcacg caaagtgccg agcggcgtaa tccgctggtg agccagttct acgctgccct
     7201 gcaattgcgc accgctttca gcgattaatg cctgcgcctg gcaagcgaga tcgagcacgc
     7261 gttgtggccc gagaagagag taaggatgtg caggtgcctg ctgacgcaaa gattccagtc
     7321 cactgtgaat gtctgccacg ggttgaccat ctggcagatg gctcagcaaa tccagcgaac
     7381 cggatgagaa atgcaacgcg ctttgaccac gcgtgacaat ggcacagcgc aggccgtgtt
     7441 tttgcagttg caggccacag agtaatccgg cgaggccgcc gcccataatg acagtatcaa
     7501 agcgcatctt tctgctcctt ctccagacca cacagtccct gataaaccca gcgggtaaat
     7561 tcgctttcgc gcagtgcgtc tccccaggcg atgggttgca cgcctttcca gcgttcgtta
     7621 aggaaggtgg aaagttgctc gatagattgc gcggacgtcg tgacgttaaa ccgttgcagc
     7681 agtccggcag cgcggcaggc gcagagttcg ccctggcagg tgcccatccc cacacgggta
     7741 cgacggcgta aatccagcag gctattaacg tttaaatttt ctaccgcgta ctgcacttca
     7801 cccgcagtta ccgcttcgca ctcacatacc aggctacggt gtagacggcc ttcgctcagc
     7861 caggcaggcg tgcgatcgcc atgacgataa accgcagaac cgcgcagcgg ggcaggcagg
     7921 gagatgactt tacgcaaggt aacttcagcg ggttcttgtg aaccaggcag tgccagatcg
     7981 gcagtcgtac aggggcgcgt gttgcccagt ttgcggcata ccgcgtcggt agcccattca
     8041 gccatcagcc gataggtcat cagtttgcca ccggtgatgg tgataaatcc gtccagacca
     8101 tcgcgttcag catggtcgag cagcacgatg ccacggctga cgttacgtcc gctcgggtcg
     8161 tcatcgctgg caaccagcgg gcgcacgcca gaataggccc gcaaaatgcg cgttttcgcc
     8221 atcacggggg ccagtttttc cccttcacgc agcagaatat caacctcttc tgccgtcact
     8281 cgattatcgt caatctcgtt gtaatcaata cgtaaagagg tggtaccaat cagcgaaatg
     8341 gtatcgccag gcaccagaat atcggcgtcg gaaggtttac ggcagcggtt gatcacatgc
     8401 tggttaatgc ggtgatccat gatcagcagc gatcctttcg ccgggaacat gcgaatgcgc
     8461 agatcggcat attcggcaat gtgttgcccc cagatcccag cggcattaac cacgacaggt
     8521 gcatgaaggg cctgagtttc gccggtgaga tggttacgta cacgaacacc gcacaccgtc
     8581 gcgccttcac gaatcagccc cgtgacttca tgagcggtaa ggataacggc accgtgttct
     8641 ttggcatcca gcatgtttgc tgcggtcaga cgaaatggat caacggtgcc atccggaact
     8701 ttcaccgcgc caatcagtgc cgggttaacg gcaggttcga taatgcgcgc ttgctgcggg
     8761 tctatagctt ctgcgctgat ccctgcttct tcgcaggcgc gaataaaagt ggcctggaat
     8821 gagaggtcat cttccggcag ggtgataaac aggccgttgg ttggttcaac gcagtgacgt
     8881 gcaatgcgtt tcaggatctg gttttcacta atgcattcgc gggccgattc cgcatcggtt
     8941 actgcatagc gcgcaccgct gtgcagcagg ccgtggttac gcccggtggc accggttgcg
     9001 atgtcgtggc gctcaaccaa aatcacgcgc agcccgcgca gggcacagtc gcgggcaatc
     9061 ccggctcccg ttgcgccgcc gccaatgata atcacgtcac ttgattgcga gtcgcgagtt
     9121 ttcattgtta tccctctgaa gttcgttttt taccatttag ccatagtaaa aacatgaatt
     9181 gtttgatttc gcgcatattc gctcatgatt cgaaagtaaa acgtgatttc atgcgtcatt
     9241 ttgaacattt tgtaaatctt atttaataat gtgtgcggca attcacattt aatttatgaa
     9301 tgttttctta acatcgcggc aactcaagaa acggcaggtt ctctcactga atcaggctgt
     9361 taatcataaa taagaccacg ggccacggag gctatcaatg ttgagtattt ttaaaccagc
     9421 gccacacaaa gcgcgcttac ctgccgcgga gatcgatccg acttatcgtc gattgcgctg
     9481 gcaaattttc ctggggatat tctttggcta tgcggcttac tatttggttc gtaagaactt
     9541 tgcgcttgct atgccttatc tggttgagca gggattctca cgcggtgatt taggttttgc
     9601 cctttcgggg atctcgattg cttatggatt ttcgaaattc atcatgggtt cggtatcgga
     9661 tcgctcgaat ccgcgcgttt tcctgcccgc aggtttgatt ctggcggcgg cagtgatgtt
     9721 gtttatgggc tttgtgccat gggcgacgtc gagcattgcg gtgatgtttg tactgttgtt
     9781 cctctgcggt tggttccagg ggatggggtg gccgccgtgt ggtcgtacta tggtgcactg
     9841 gtggtcgcag aaagaacgtg gcggcattgt gtcagtgtgg aactgtgcgc acaacgtcgg
     9901 tggtggtatt ccgccgctgc tgttcctgct ggggatggcc tggttcaatg actggcatgc
     9961 ggcgctctat atgcctgctt tctgcgccat tctggtggca ttattcgcct ttgcgatgat
    10021 gcgcgatacc ccgcaatcct gtggcttgcc gccgatcgaa gagtacaaaa atgattatcc
    10081 ggacgactat aacgaaaaag cggaacagga gctgaccgcg aagcaaatct tcatgcagta
    10141 cgtgctgccg aacaaactgc tgtggtacat cgccatcgcc aacgtgttcg tttatctgct
    10201 gcgttacggc atcctcgact ggtcaccgac ttatctgaaa gaggttaagc atttcgcgct
    10261 ggataaatcc tcctgggcct acttcttcta tgagtatgcg ggtattccgg gcaccctgct
    10321 gtgcggctgg atgtcggaca aagtcttccg tggtaaccgt ggggcaaccg gcgtcttctt
    10381 tatgacactg gtgaccatcg cgactatcgt ttactggatg aacccggcag gtaacccaac
    10441 cgtcgatatg atttgtatga ttgttatcgg cttcctgatc tacggtcctg tgatgctgat
    10501 cggtctgcat gcgctggaac tggcaccgaa aaaagcggca ggtacggcag cgggctttac
    10561 cgggctgttt ggttacctgg gcggttcggt ggcggcgagc gcgattgttg gctacaccgt
    10621 ggacttcttc ggctgggatg gcggctttat ggtaatgatt ggcggcagca ttctggcggt
    10681 tatcttgttg attgttgtga tgattggcga aaaacgtcgc catgaacaat tactgcaaaa
    10741 acgcaacgga ggctaatggc atgaaattga agctgaaaaa ccttagcatg gcgatcatga
    10801 tgagcactat agtcatggga agcagtgcaa tggcggcgga cagcaacgaa aaaatagtca
    10861 tcgcccatcg cggtgccagt ggatatttgc cggagcatac gctgccagca aaagcgatgg
    10921 cgtatgcgca gggagcggat tatctggaac aggatttggt gatgaccaaa gacgaccatc
    10981 tggttgttct gcatgaccat tatctcgatc gtgttactga tgttgccgat cgtttcccgg
    11041 atcgggcgcg caaagacggt cgttactacg cgatagattt cacgctggat gaaattaagt
    11101 cgctgaaatt taccgaaggt ttcgatattg aaaacggtaa aaaagtacag acttatccgg
    11161 ggcgtttccc aatgggtaag tccgacttcc gggtgcacac ctttgaagaa gagattgaat
    11221 ttgttcaggg gttaaatcac tctaccggga aaaatatcgg tatctatcca gaaatcaaag
    11281 cgccgtggtt ccatcatcag gaagggaagg atattgcggc aaaaacgctg gaagtgctga
    11341 agaaatatgg ttacaccggt aaagacgata aagtttattt gcaatgtttt gatgctgatg
    11401 agctgaagcg tattaagaat gagctggaac ccaaaatggg catggagctc aatctggtac
    11461 agctgattgc ctataccgac tggaatgaaa cgcagcagaa acagccggat ggaagctggg
    11521 ttaattacaa ctacgactgg atgtttaagc cgggagccat gaaacaggtg gcggaatatg
    11581 cagatggtat tggtccggat taccatatgt tgattgagga gacatcgcag ccgggtaata
    11641 tcaaactcac tggcatggtg caagatgccc agcagaataa actggtagtg catccttata
    11701 ccgtgcggtc agataaactg cctgaataca ctcctgatgt gaatcagtta tatgatgctc
    11761 tgtataacaa agcgggtgta aatgggctgt ttactgattt ccctgataag gcagtaaaat
    11821 ttcttaataa agagtaatgt tactgcagga tggcgttcat gcgccatcct gtctcatatc
    11881 aaatttttcc aatcgccact aattgttcca tgcacatttg tagtaaaaac agtggactgc
    11941 cttctaccgg cttttcttta gtcagcaacg aaatctgtcg atagaaggtt ggttcgtgag
    12001 gaacggaaca caattcacct gcaatgcttt tcagcgttaa ctctggttgt aaggcgatgc
    12061 ctaatccctg tcgaataaaa ttcatcgctg tatccgactg attaaattca cattttatct
    12121 gctgggcgca ctattagtgg tagtggctgg tggaatctat gcgctgcaaa tgaagggcaa
    12181 aagcggtgtc acacctgtag cggcaaaaga aattcattaa tacgacacgt ttcattaaga
    12241 ttttcctcag gtaaccacct atagtcattg tcgttaataa aaatgacagg cgatagggta
    12301 tggcagtttc agcaaagtac gacgaattta atcactggtg ggcaacagag ggcgactggg
    12361 ttgaagagcc taactatcgc cgtaatggta tgagcggggt gcaatgcgtc gagcgcaacg
    12421 gcaaaaagct gtatgtaaag cgcatgacgc atcatctgtt tcattcagta cgttatccgt
    12481 tcggccgacc aacgattgtc cgtgaggttg cggtaattaa agaactggaa cgggccggtg
    12541 tcatcgtgcc gaagatcgtt tttggtgaag cggtgaaaat tgagggtgaa tggcgcgcgt
    12601 tgctggtgac tgaagatatg gcggggttca tcagcattgc tgactggtat gcccggcatg
    12661 cagtatcgcc ttattctgac gaagtacggc aagccatgtt gaaagcagtg gcactggcgt
    12721 ttaagaaaat gcatagcgtt aatcgtcagc atggctgttg ctatgttcgc catatctatg
    12781 tgaaaacaga aggtaaggca gaagctggtt ttcttgatct ggagaaaagt cgtcgccgct
    12841 tgcgtcgcga taaagcgatt aatcatgact tccgccagct ggaaaaatat ctggagccga
    12901 tccctaaagc ggactgggag caggtgaaag cgtattacta cgccatgtaa tcgttgtagg
    12961 ccagataata cgcgtaagcg tcgtatctgg cgctagtacc cgatcacaac tcgatttcaa
    13021 tatcgccttt tgcccggcaa caacagggca aaatttcccc cggctgaata aaggctaacg
    13081 gttcggcaat ccagtccacc tgacctgcga ccagtcgggt gcgacaggag ccgcagtaac
    13141 cttcgcggca ctggtactca accgccacat tgtgggattc cagcgccgcc agaagggaag
    13201 ggtgttcatc ctggcacagc agttgtgtgc cagtgatgcg cagggtaacg cgggccatca
    13261 gagctggaag ttactcaaat cgtcggtgtc cacttccgcg tcaatctgcc cgaccagata
    13321 agaactgact tccacttcct gcggagcaac ctgcacgtta tcagacacca gccaggtgtt
    13381 gatccacggg atcgggttgg agcgcgtctg gaacggcaga tccaaaccga ctgcctgcat
    13441 gcggatattg gtgatgtatt caacgtactg gcagagaata tctttattca gaccaatcat
    13501 cgaaccgtca cggaacagat aatccgccca gtctttctct tgctgtgccg cctggacaaa
    13561 caggtcatag cactcctgct tacattcttc ggcaatttcc gccatctccg gatcatccgc
    13621 gccgctgcgc agcagattca gcatatgctg agtaccggtc aggtgcaggg cttcatcgcg
    13681 ggcaatcagg cgaataatct tggcgttacc ttccatcagt tcgcgttctg caaaggcaaa
    13741 ggaacaagca aagctgacgt agaagcggat cgcttccagc gcgttaacgc tcatcaggca
    13801 gagatacagt tttttcttca gctcgcgcag gctaacggtt acggttttac cgttaacggt
    13861 gtgggtgcct tcgcccagaa ggtgccagta gctggtcatt tcgatcagtt catcgtaata
    13921 gctggagatc ccttccgcac gtttctggat ctgctcgttg gtgacgatat cgtcaaacac
    13981 aacagacgga tcgttaacga tattacgaat gatatgagta taggaacggg aatgaatcgt
    14041 ttctgagaac gcccaggttt cgacccaggt ttccagttcc ggaatagaaa taagcggcaa
    14101 tagcgccacg ttcgggctac gaccctgaat ggaatccagc agcgtctgat atttcaggtt
    14161 gctgataaag atgtgttttt cgtgctccgg cagcgcctgg taatctatac ggtcgcggga
    14221 gacgtcaact tcttccggac gccagaagaa agagagctgc ttttcgatca gcttttcgaa
    14281 gatgtcatat ttttgctgat cgtagcgagc cacgttgacc ggctgaccaa agaacatcgg
    14341 ttctttgagc tgatcatttt tcgtctgtga aaaggtggta tatgccatga gtgtgtcctg
    14401 ttgggagttt aatgccggat aaggcatttt acgccgcatc ctgtgcctga tgcgacgctg
    14461 gcgcgtctta tcaggcctac aaatccgagc cgtaggccgg ataaggcgtt cacgctgcat
    14521 ccggcacccg gagcctgatg cgacgctggc gcgtcttatc aggcctacaa accgagccgt
    14581 aggccggata aggcgtttac gccgcatccg gcatctcaat atcagatctt acatgcgccg
    14641 ctttcgcagc catcgtcctg gattgacggt accagatcgt cctgcgcgtc ttcagcgccg
    14701 tcacgggtgt tctgataata cagcgttttg accccgaatt tgtaggcggt gagcaggtct
    14761 ttcagcaact gctgcatcgg cacttttcct gacgggaaac gtgacggatc gtagttggtg
    14821 ttagcagaga tcgactgatc gataaatttc tgcatgatac ctaccagttg caggtagcca
    14881 tcgttacccg gcatttccca cagcagctca taggcgtcgt gcaggtgctc gtagtccggc
    14941 accacctggc gcaaaatacc gtctttcgac gctttgatgc tgacgtaacc gcgcggcggt
    15001 tcaataccgt tagtggcgtt agagatctgc gaagaggtct cggacggcat cagggcagaa
    15061 agcgtggagt tacgcaggcc gtgcgttttg attgattcac gcaatgcttc ccagtcgtaa
    15121 tgcagcggct cattagcgat ggcatccaga tctttcttat aggtatcgat cggcaggatc
    15181 cctttcgcgt aagtggtttc gttaaaccac gggcacgcgc cttgctcttt agccagctca
    15241 ttagacgctt tcagcaggta atactgaatc gcttcgaagg ttttatgcgt caggttgttg
    15301 gcgctgccgt cggagtagcg tttaccgtgc ttcgccagat agtaagcgaa gttaatcaca
    15361 ccaataccca gcgtacgacg ccccatcgct ccacgtttgg cggccgggat cgggtaatcc
    15421 tgataatcca gtagcgcgtc aagtgcacga accgccagaa tcgccagctc ttccagttca
    15481 tccaggctat taattgcgcc caggttgaaa gcagacagcg tacacagcgc gatttcaccg
    15541 ttctcgtcgt tgacgtcgtt cagcggtttg gtcggcaggg caatttctag gcacaggtta
    15601 gactggcgaa ctggcgcgat gttcggatca aacggactat gggtattaca gtggtcaacg
    15661 ttctgaatat agatacgacc ggtagacgca cgttcctgca tcatcagcga gaacagctca
    15721 acggctttca cacgctgctt gcggatgctg tcgtctttct catatttggt atacaggcgt
    15781 tcaaactctt cctgatcggc gaagaacgcg tcgtacagcc ccggtacgtc ggacgggctg
    15841 aacagggtga tatcttcacc tttcagcagg cgggtataca tcagtttgtt gatttgtacc
    15901 ccgtagtcca tatgacgcac gcggttgcct tcaaccccac ggttgttttt caacaccagc
    15961 aggctttcca cttccagatg ccacatcggg tagaacagcg ttgccgcacc gccgcgcaca
    16021 ccgccctgag agcaggattt caccgctgtc tggaaatgtt tgtagaacgg gatacagccg
    16081 gtatggaacg cttcgccacc gcgaatcggg ctgcccagcg cacgaatacg tccggcgttg
    16141 atgccgatcc cggcacgctg ggaaacatat ttcacaatcg cgctggaggt ggcgttgatg
    16201 gaatccaggc tgtcaccgca ctcgatcagt acgcaggagc tgaactgacg agtcggggta
    16261 cgcacgccgg acatgattgg cgtcggcagc gaaattttaa atgtggaaac cgcgtcataa
    16321 aaacgcttca cgtattgcag gcgcgtttca cgcgggtagt tcgagaacag gcacgcggca
    16381 acaagaatat aaaggaactg ggcgctttcg tagatttcgc cggtcacgcg gttctgtact
    16441 agatatttgc cttccagctg cttaacggca gcataagaga aggtcatatc acggtcgtga
    16501 tcgataaacg tgtccatctg cttgaactct tcttccgtgt agtcttccag cagatgatta
    16561 tcgtatttgc ccatctcgac cattttcacc acgtggtcgt acagcgcagg cggctcaaac
    16621 tggccgtagg cttttttacg caggtggaag atcgccaggc gcgcggcgag atactgataa
    16681 tccggcgcat cacgggagat caggtctgcg gcagccttga taatagtttc gtggatgtca
    16741 gaggtcttga taccgtcata aaactgaatg tgggagcgca gctcgacctg ggaaatcgaa
    16801 acgttatgca gtccttctgc cgcccaatcc agaacgcgat ggattttgtc gagattgatg
    16861 cgctctgtgc taccgtcgcg ctttgtcacc agcagattct gattcatgta tgtcgtacct
    16921 gtttttggaa ttctttccgc aatagtttca tgcagactac ctgtagtaag ggaagctgct
    16981 tagaacacta tatatagggg gtatgtttgg gattcactgc aagatagtgt gaaaatgacc
    17041 ctcttgcaag tgcataactt tgtggataac tcaggaagga aaaagtggct ttcgcgcacc
    17101 ttaggtcaga caaggtgtcc gggaaagtca atgggaagag aaaaatttgt taaaaataac
    17161 tgttcggcga tttcttgaac ggcagaatag gttggataaa ttgatataga tggtttatta
    17221 atctttttgc taatgattgc aaaaattgat ctaagcacga gtcatcacat aatctgatgt
    17281 atcagttgtg gtcaatatat aagcgaatgt aagcgattgt tgatgtcgaa atggttgttt
    17341 tctattcggt tattgttaaa tgcgaagcca ttttgtta
//
LOCUS       DAZSIO010000080        17331 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZSIO010000080 DAZSIO010000000
VERSION     DAZSIO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.080"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..2095
                     /gene="pbpC"
                     /locus_tag="ACHX5T_002404"
     CDS             <1..2095
                     /gene="pbpC"
                     /locus_tag="ACHX5T_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFR8467570.1"
                     /translation="ARYLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGS
                     TLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGA
                     ASWAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWS
                     REQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNW
                     KGRLPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVY
                     GLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEA
                     YGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLR
                     LQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWA
                     IGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVT
                     RGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQ
                     RVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGV
                     RDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLV
                     MDDVGQIATVKFVMQ"
     gene            2245..2676
                     /gene="ndk"
                     /locus_tag="ACHX5T_002405"
     CDS             2245..2676
                     /gene="ndk"
                     /locus_tag="ACHX5T_002405"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR8467571.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2826..3980
                     /locus_tag="ACHX5T_002406"
     CDS             2826..3980
                     /locus_tag="ACHX5T_002406"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR8467572.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4265..5278
                     /gene="rodZ"
                     /locus_tag="ACHX5T_002407"
     CDS             4265..5278
                     /gene="rodZ"
                     /locus_tag="ACHX5T_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR8467573.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5305..6432
                     /gene="ispG"
                     /locus_tag="ACHX5T_002408"
                     /gene_synonym="gcpE"
     CDS             5305..6432
                     /gene="ispG"
                     /locus_tag="ACHX5T_002408"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR8467574.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6535..7809
                     /gene="hisS"
                     /locus_tag="ACHX5T_002409"
     CDS             6535..7809
                     /gene="hisS"
                     /locus_tag="ACHX5T_002409"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR8467575.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7827..8447
                     /gene="yfgM"
                     /locus_tag="ACHX5T_002410"
     CDS             7827..8447
                     /gene="yfgM"
                     /locus_tag="ACHX5T_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR8467576.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8458..9636
                     /gene="bamB"
                     /locus_tag="ACHX5T_002411"
     CDS             8458..9636
                     /gene="bamB"
                     /locus_tag="ACHX5T_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR8467577.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9754..11226
                     /gene="der"
                     /locus_tag="ACHX5T_002412"
     CDS             9754..11226
                     /gene="der"
                     /locus_tag="ACHX5T_002412"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR8467578.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11296..11511
                     /locus_tag="ACHX5T_002413"
     CDS             11296..11511
                     /locus_tag="ACHX5T_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR8467579.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="ACHX5T_002414"
     CDS             complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="ACHX5T_002414"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR8467580.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13040..14506
                     /gene="guaB"
                     /locus_tag="ACHX5T_002415"
     CDS             13040..14506
                     /gene="guaB"
                     /locus_tag="ACHX5T_002415"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR8467581.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14575..16152
                     /gene="guaA"
                     /locus_tag="ACHX5T_002416"
     CDS             14575..16152
                     /gene="guaA"
                     /locus_tag="ACHX5T_002416"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR8467582.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16247..16786)
                     /locus_tag="ACHX5T_002417"
     CDS             complement(16247..16786)
                     /locus_tag="ACHX5T_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR8467583.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16802..17320)
                     /locus_tag="ACHX5T_002418"
     CDS             complement(16802..17320)
                     /locus_tag="ACHX5T_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR8467584.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
BASE COUNT         4005 a         4314 c         4820 g         4192 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgta ataaagtgct cgaacggatg gctgcgcaag gtgtgtggtc
      481 ccgtgagcag gtaaaagagt caagggaaga acccatctgg ctggcacccc gacaaatgcc
      541 gcaactggca ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt
      601 tactacgctg gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg
      661 gcggttgcca ccgcgcagct cactggcgat gatcgtggtt aatcataccg atatgcgtgt
      721 tcgcggctgg gtgggatcgg ttgatctcaa cgatgattca cgctttggtc atgttgatat
      781 ggtcaatgcg atccgatcgc caggatcggt gctcaaaccg tttgtttatg gtctggcgct
      841 ggatgaaggc ttgatccacc cggcatcact gctgcaagac gtcccccggc gcaccggtga
      901 ttatcgacca ggtaactttg atagcggttt tcatggcccg atcaacatga gcgaggcgct
      961 ggtgcgctcg ctgaacttac ctgctgtgca ggtgctggaa gcctatggac cgaaacggtt
     1021 tgcggcaaag ttacgcaatg ttggattgcc gttatatttg cccaacggcg ctgcgccgaa
     1081 tctttcactc attctcggcg gcgctggtgc aaaactggaa gatatggcgg cagcgtatac
     1141 cgcgtttgct cgccacggca aggcaggcaa attgcgctta cagcctgacg atccgctgct
     1201 tgaacggcct ttaatgtcgt cgggggcggc gtggatcatt cgccggatta tggctgatga
     1261 agcgcaaccc ttgccggata gtgccttgcc gcgcgtcgcc ccactggcat ggaaaacggg
     1321 caccagctat ggctatcgtg acgcctgggc gattggggtt aacgctcgct atgtcattgg
     1381 gatctggact ggcagacctg acggcacgcc cgttgttggt cagtttggct ttgccagtgc
     1441 cgtaccattg ttgaatcagg tcaataatat cttactgtcg cgcagtgcga atctgccgga
     1501 agacccgcgc ccgaactcag tcactcgtgg cgttatctgc tggccgggcg gacagtcttt
     1561 gccggaaggt gacggtaact gtcgccgccg cctggcaacc tggctgctgg atggcagcca
     1621 gccgccaacg ctgttactgt cagagcagga agggattaat ggcattcgct tccccatctg
     1681 gctggatgac aaagggcagc gcgtcgccgc tgactgcccg caggcgaagc aagaaatggt
     1741 aaatgtctgg ccgctaccgc tggaaccctg gctgcccgca tcagaacgtc gcgctgtgcg
     1801 cttaccaccg gcctcgacaa tttgcccgcc atacggtcac gatgcccagc tccctctgca
     1861 gctgacaggc gttcgcgatg gcgcgattat taaacgttta ccgggcgcag cggaagcgac
     1921 tttgccgttg caatcaagtg gaggggcagg tgaacgctgg tggtttctga atggcgaacc
     1981 gttaactgaa cgcgggcgca acgtgaccct gcatttgacg gataaaggtg attatcaatt
     2041 gctggtgatg gatgatgtgg ggcaaatcgc gacagtgaaa tttgtcatgc aatagtctgt
     2101 tttgtccctg attgttgcta aaactcattt tattttaaaa aaaatgttac cttcatcaat
     2161 agtcaacggc cctgttgctc attataatcc gcgccatctc gtacgctggt acagacaaca
     2221 acagaacaat ttacagaggt aaaaatggct attgaacgta ctttttccat catcaaaccg
     2281 aacgcggtag caaaaaacgt cattggtaat atctttgcgc gctttgaagc tgcagggttc
     2341 aaaattgtcg gcaccaaaat gctgcatctg accgttgaac aggcacgtgg cttttatgct
     2401 gaacacgatg gaaaaccgtt ctttgatggt ctggttgaat tcatgacctc tggcccgatc
     2461 gtggtttccg tgctggaagg tgaaaacgcc gttcagcgtc accgcgatct gctgggcgcg
     2521 accaatccgg caaacgcact ggctggtact ctgcgcgctg attacgctga cagcctgacc
     2581 gaaaacggta cccacggttc tgattccgtc gaatctgccg ctcgcgaaat cgcttatttc
     2641 ttcggcgaag gcgaagtgtg cccgcgcacc cgttaataat ttcgtaaatg ccgcgtgcaa
     2701 acgtggcatc cgtgcgccag aatttgtaca atgcagcgcc cccggacgag cagccgctca
     2761 ccggggcgtt tcttttttca accctccagg ggccataacg tgtaataacg aggccggaat
     2821 agcatatgtc tgaacaatta gtcacacctg aaaacgtcac cacgaaagat ggaaaaatca
     2881 acctgctgga tctcaaccgt cagcagatgc gggagttttt taaagattta ggtgaaaaaa
     2941 ccttccgcgc cgatcaggtg atgaagtgga tgtatcacta ttgctgcgac aactttgatg
     3001 agatgaccga catcaacaaa gtgttgcgcg gcaaactgaa agaggtggcg gaaatccgcg
     3061 caccggaagt ggttgaagaa cagcgttcat ccgacggcac cattaaatgg gcgatcgccg
     3121 ttggcgatca gcgcgtcgaa acggtgtata tcccggaaga cgaccgtgcc acgctctgcg
     3181 tctcttcgca ggtggggtgt gcgctggagt gtaaattctg ttccaccgcc cagcagggct
     3241 ttaaccgcaa cctgcgggta tcggaaatta ttggccaggt gtggcgtgcg gcgaaaatcg
     3301 tcggcgcagc aaaagtcacc ggtcagcgcc caatcactaa cgtcgtgatg atgggcatgg
     3361 gcgagccgct gctcaacctg aacaacgtcg ttccggcgat ggaaatcatg cttgatgatt
     3421 tcggttttgg cctgtctaaa cgtcgcgtga cgctttccac ttccggtgta gttccggcgc
     3481 tggataaact gggcgatatg atcgacgttg cactggcaat ttccctgcac gcgccgaacg
     3541 acgaaattcg tgacgaaatt gtgccgatca acaaaaagta caatatcgaa acgttccttg
     3601 ctgcggtgcg ccgttatctg gagaaatcca acgccaatca gggccgagtc actattgaat
     3661 acgtgatgct tgaccacgtt aacgacggca ctgaacacgc gcaccaactg gcggaactgc
     3721 tgaaagatac gccgtgtaag atcaacctga tcccgtggaa cccgttcccg gacgcgccgt
     3781 atggacgcag ttcgaacagc cgtatcgacc gtttctcaaa agtgctgatg agctacggtt
     3841 tcaccactat tgtgcgtaaa acgcgtggtg atgatatcga tgctgcctgt ggtcagttgg
     3901 cgggcgatgt tatcgaccgt acgaaacgta ctctgcgtaa acggatgcag ggtgaagcca
     3961 tcgatattaa agcggtctga tagccattac gccacggtta ctttatgtac cgtggcgtaa
     4021 tcttttatga atgaagtatt aagttgcaat tgtctggtca atattaacgg tgcgtttttg
     4081 ctgactttaa ggcagtatgt aacgatgcaa cagtaagtta gcctcagtga atgtgggctt
     4141 tgtcacgagc acacagacgg tcttatactg tatgataacg gttaacttaa cggatgtttc
     4201 gcggtgtggg tgagcatgat ggttcaccgg catctcaatt ctcatttaaa cgtacctgca
     4261 gcgaatgaat actgaagcca cgcacgacca aaatgaagca cttactaccg gcgctcgcct
     4321 gcgtaatgct cgcgaacaac taggacttag tcagcaggcc gttgccgagc gactttgcct
     4381 gaaggtttcc acggtacgcg acattgaaga agataaggca cccgccgatc ttgcttcaac
     4441 attcctgcgc ggatatatcc gctcttatgc gcgtctggta catattccag aagaagaact
     4501 gctgccaggg ctggaaaagc aggctccact tcgggctgca aaagttgcgc caatgcagag
     4561 tttttccctc ggtaaacgcc gcaaaaaacg cgacggctgg ctaatgacct tcacctggct
     4621 ggtgttgttt gtggttatcg gcctgagcgg tgcctggtgg tggcaagacc acaaagctca
     4681 gcaggaagag atcaccacta tggccgatca atcttcggcg gaactgagca gtaatagcga
     4741 gcaggggcag agtgttccgt taaatacgtc gacaactaca gacccggcta caacgtccac
     4801 gccgccagcg tctgtggata ctaccgcaac caacacgcaa acacctgccg taactgcgcc
     4861 agcaccagct gttgatccgc aacagaatgc ggttgtttcg ccttcgcagg caaatgttga
     4921 taccgccgcg accccggtac caacggcagc aacaacgcca gatggtgctg cgccgttgcc
     4981 aaccgatcag gctggcgtga ccacgccggc ggctgatccg aatgcgctgg tgatgaactt
     5041 tactgccgat tgctggctgg aggtcactga tgctaccggt aaaaaattgt ttagcgggat
     5101 gcagcgtaaa gacggtaatt tgaatttaac cggccaggca ccgtataaac tgaaaattgg
     5161 tgcgccagcc gcagtacaga tccagtatca agggaaacct gtcgatctga gtcgttttat
     5221 cagaactaac caggttgcgc gtctgaccct caatgccgaa caatcaccgg cgcagtaaca
     5281 gacgggtaac gcgggagatt tttcatgcat aaccaggctc caattcaacg tagaaaatca
     5341 acacgtattt acgttgggaa tgtgccgatt ggcgatggtg ctcccatcgc cgtacagtcc
     5401 atgaccaata cgcgtacgac agatgtcgaa gcaacggtca atcaaatcaa ggcgctggaa
     5461 cgcgttggcg ctgatatcgt ccgtgtctcc gtaccgacga tggacgcggc agaagcgttc
     5521 aaactcatca aacagcgggt taacgtgccg ctggtggctg acatccactt cgactatcgc
     5581 attgcgctga aagtagcgga atacggcgtc gattgtctgc gtattaaccc tggtaatatc
     5641 ggtaatgaag agcgtattcg catggtggtt gactgtgcgc gcgataaaaa cattccgatc
     5701 cgtattggcg ttaacgccgg atcgctggaa aaagatctgc aagaaaagta tggcgaaccg
     5761 acgccgcagg cgttgctgga atccgccatg cgtcatgttg atcatctcga tcgcctgaac
     5821 ttcgatcagt tcaaagtcag cgtgaaagcg tctgacgtct tcctcgctgt tgagtcttat
     5881 cgtttgctgg caaaacagat cgatcagccg ctgcatctgg ggatcaccga agccggtggt
     5941 gcacgcagcg gggcagtaaa atccgccatt ggtttaggtc tgctgctgtc ggaaggcatc
     6001 ggcgacacgc tgcgtgtatc gctggcggcc gatccggtcg aagagatcaa agtcggtttc
     6061 gatattttga aatcgctgcg tatccgttcg cgagggatta acttcatcgc ctgcccgacc
     6121 tgttcgcgtc aggaatttga tgttatcggt acggttaacg cgctggagca acgcctggaa
     6181 gatatcatca ctccgatgga cgtttcgatt atcggctgcg tggtgaatgg cccaggtgag
     6241 gcgctggttt ctacactcgg cgtcaccggc ggcaacaaga aaagcggcct ctatgaagat
     6301 ggcgtgcgca aagaccgtct ggacaacaac gatatgatcg accagctgga agcacgcatt
     6361 cgtgcgaaag ccagtcagct ggacgaagcg cgtcgaattg acgttcagca ggttggaaaa
     6421 ataataacgt gatgggaagc gcctcgcttc ccgtgtatga ttgaacccgc atggctcccg
     6481 aaacattgag ggaagcgttg agggttcatt tttatattca gaaagagaat aaacgtggca
     6541 aaaaacattc aagccattcg cggcatgaac gattacctgc ctggcgaaac ggccatctgg
     6601 cagcgcattg aaggcacact gaaaaacgtg ctcggcagct acggttacag tgaaatccgc
     6661 ttgccgattg tagagcagac cccgctattc aaacgtgcga ttggtgaagt caccgacgtg
     6721 gttgaaaaag agatgtacac ctttgaggat cgcaatggcg acagcctgac tctgcgccct
     6781 gaagggacgg cgggctgtgt acgcgccggc atcgagcatg gtcttctgta caatcaggaa
     6841 cagcgtctgt ggtatatcgg gccgatgttc cgtcacgagc gtccgcagaa agggcgttat
     6901 cgtcagttcc atcagttggg ctgcgaagtt ttcggtctgc aaggtccgga tatcgacgct
     6961 gaactgatta tgctcactgc ccgctggtgg cgtgcgctgg gtatcttcga acacgtaact
     7021 cttgagctga attctatcgg ttcgctggaa gcacgcgcca attaccgcga tgcgctggtg
     7081 gcattccttg agcagcataa agaaaagctg gacgaagact gcaaacgccg catgtacact
     7141 aacccgctgc gcgtgctgga ttcaaaaaat ccggaagtgc aggcgcttct caacgacgct
     7201 ccggcattag gtgattatct ggacgaggaa tctcgtgagc attttgccgg tctgtgcaaa
     7261 ctgctggaga gcgcggggat cgcttacacc gtaaaccagc gtctggtgcg tggtctggat
     7321 tactataacc gtaccgtttt cgagtgggtg actaacagtc tcggctccca gggcaccgtg
     7381 tgtgcaggcg gtcgttatga cggtcttgtg gaacaactgg gcggtcgtgc aacaccggct
     7441 gtcggttttg cgatgggcct cgaacgtctt gtattgttag tacaggccgt taatccggaa
     7501 tttaaagccg atcctgttgt cgatatatac ctggtggctt caggtgctga tacacaatct
     7561 gcggctatgg cattagctga gcgtctgcgt gatgaattac tgggcgtgaa attgatgacc
     7621 aaccacggcg gcggcaactt taagaaacag tttgcccgtg ctgataaatg gggtgcccgc
     7681 gttgctgtgg tgctgggtga gtctgaagtg gctaacggca cagcagtagt gaaggatttg
     7741 cgctctggtg agcaaacggc agttgcgcag gatagcgtag ccgcgcattt gcgcacgtta
     7801 ctgggttaag gaaggagaag gacagcgtgg aaatttacga gaacgaaaac gaccaggtag
     7861 aagcggttaa acgctttttt gctgaaaatg gcaaagcact ggctgttggg gggattttgg
     7921 gcgttggcgc actgactggc tggcgctact ggaacagcca tcaggttgat tctgcacgct
     7981 ccgcttctct tgcctatcaa aatgcggtta ccgcagtgag cgaaggcaaa ccggatagca
     8041 tcccggcggc ggaaaaattt gctgctgaaa ataaaaatac ttatggtgcg ctggcttctt
     8101 tggaacttgc gcagcaattt gttgacaaaa atgaactaga gaaagctgcc gcccagttac
     8161 aacaggggct ggcagacacg agcgatgaaa atctcaaagc cgtgataaat ctgcgtcttg
     8221 ctcgcgttca ggtacagctc aagcaggctg atgccgcgct gaaaaccctt gataccatca
     8281 aaggtgaagg gtgggctgcc attgttgccg acttgcgtgg tgaagcattg ctgagcaaag
     8341 gtgataagca aggtgcgcgt agtgcatggg aagcaggcgt gaaaagcgat gttactccgg
     8401 cactgagcga aatgatgcag atgaaaatta ataatttgtc catctgagag ggacccgatg
     8461 caattgcgta aattactgct gccaggactg ctttccgtta cccttttaag cggctgttcg
     8521 ctgtttaaca gcgaagaaga tgtggtaaag atgtccccat tgccaaccgt tgaaaaccag
     8581 tttacgccga ccacggcgtg gagcacttcc gttggtagcg gcattggcaa cttctattcc
     8641 aatcttcatc cggcactggc ggacaacgtt gtctatgcag cggaccgcgc tggtttggta
     8701 aaagcgctga atgcggatga tggcaaagaa atctggtctg tcagcctggc cgagaaagat
     8761 ggctggttct ctaaagagcc tgcattactt tctggcggtg tgaccgtgtc tggtgggcat
     8821 gtctacattg gcagcgaaaa ggcgcaggtt tacgcgctga ataccagcga tggtactgtg
     8881 gcatggcaaa ctaaagtcgc gggtgaagca ctttcgcgcc cggtggtcag cgacggtctg
     8941 gtgttaatcc acaccagtaa cggtcagtta caagcgctga acgaagctga cggcgctgtc
     9001 aaatggacag ttaacctcga tatgccttcg ctctctttgc gtggcgagtc tgcgccggca
     9061 acggcttttg gtgcggccgt cgtgggtggc gataatggtc gcgtcagcgc agtgctgatg
     9121 gaacagggcc agatgatttg gcagcagcgt atttcccagg cgaccggttc taccgaaatt
     9181 gaccgtctga gcgatgttga cacgactccc gtcgttgtta acggcgttgt tttcgcgctg
     9241 gcctataatg gtaacctgac ggcgcttgat ctgcgcagtg gtcagattat gtggaaacgc
     9301 gaactgggtt cggtgaatga tttcatcgtc gatggcaatc gcatctatct ggtcgatcaa
     9361 aatgaccggg tgatggcgtt gaccattgat ggcggcgtta cgctgtgggc acaaagcgat
     9421 ctgctgcatc gcctgctgac ttctccggtg ctgtataatg gcaacctggt ggtcggtgac
     9481 agtgaaggtt atctgcactg gattaacgtc gaagatggtc gtttcgttgc ccagcaaaaa
     9541 gttgatagtt ccggtttcca gactgaaccg gttgccgctg acggcaaact gctgatccag
     9601 gcaaaagacg gaaccgtgta ctctattaca cgttaatcgt ctctgtcgtt cactttgaaa
     9661 acggctcctg gacaggggcc gttttcctgt ttttaacaac gacgcgaata tagcgtgcgt
     9721 tgtctgatga tttataaaaa tgaggcttta aacatggtac ctgtggtcgc gcttgtcggg
     9781 cgccctaacg taggaaaatc cacgttattt aaccgtctaa ctcgcacccg agatgcgctg
     9841 gttgcggatt tcccgggtct gactcgtgac cgtaagtacg gtcgtgcgga aattgaaggc
     9901 cgtgagttta tctgtattga taccggcggg attgatggca cagaagacgg tgtagaaacc
     9961 cgcatggcgg aacagtcgct gctggcgatt gaagaagcgg acgtcgtact gtttatggtg
    10021 gatgcgcgcg cgggcctgat gccggcagat gaagcgattg ccaaacatct gcgctcccgt
    10081 gaaaaaccga ccttcctggt ggtaaacaaa actgacggtc tggatcccga tcaggcagtg
    10141 gttgatttct actcgcttgg tttaggtgaa atctacccga tcgccgcgtc tcacggtcgt
    10201 ggcgtattaa gtctgctgga gcatgtgctg ctgccgtgga tggaagatct cgcaccgcaa
    10261 gaggaagtcg acgaagacgc tgaatactgg gcgcaatttg aagcggaaga gaacggcgaa
    10321 gaagaagagg aagacgactt cgacccgcaa agtctgccga tcaaactggc gattgtgggt
    10381 cgtccgaacg taggtaagtc tacactcact aaccgtattc ttggagaaga gcgcgttgtt
    10441 gtttacgaca tgcctggcac gacgcgtgac agcatctaca tcccaatgga acgcgatgga
    10501 cgtgagtatg tgctcattga caccgctggc gtacgtaaac gcggcaaaat caccgatgct
    10561 gtagagaaat tctccgtaat caaaacgttg caggccattg aagacgccaa cgtggtgatg
    10621 ttagtgattg atgcgcgcga aggtatttcc gatcaggatc tctcgctgct gggctttatt
    10681 ctcaatagtg ggcgctcact tgtcattgtg gtgaataagt gggatggcct gagccaggaa
    10741 gtgaaagagc aggtgaaaga aacgctggac ttccgtctgg gctttatcga ttttgctcgt
    10801 gtgcacttta tctctgcctt gcacggcagt ggtgttggta acttgtttga atcagtacgt
    10861 gaagcgtatg acagctccac ccgtcgtgtg gggacctcta tgctgacgcg catcatgacg
    10921 atggctgttg aagatcacca accgccgctg gtacgcggtc gtcgtgtgaa gctgaaatat
    10981 gcccacgctg gtggttataa cccgccgatt gtggtgattc acggtaatca ggtgaaagac
    11041 ctgcctgatt cctacaagcg ctacttgatg aactacttcc gcaaatcgct ggacgtaatg
    11101 ggatcgccga ttcgtattca gttcaaagaa ggggaaaacc cgtatgcgaa caagcgtaac
    11161 accctgacgc caacccagat gcgtaaacgt aagcgtctga tgaagcacat caagaaaaat
    11221 aaataattat ccgtaagtat tttatctgcc attccctcta tgaatgtaga gggaatggct
    11281 accggaggaa ttcatatgga actgcattgt ccaaaatgtc agcatgtgct tgatcaggat
    11341 aacggccatg cgcgttgccc atcctgcggg gaatttattg aaatgaaagc tctttgtcca
    11401 gactgccatc aaccgttgca ggtgttaaaa gcctgcggtg cagtagatta cttctgtcag
    11461 cacgggcatg gcttgatatc gaaaaaacgc gttgaattcg tgctggctta atgaaccttt
    11521 ttacgcgatt tttttaccgg ctggatgttt tttacttcac tttctatcca gccgtcttcc
    11581 agacgtgtgg ttagcatttc acccgtttta acttgcttca cttttttcag tacattgccg
    11641 tcagtagcag tagtaacgct gtatccacgc gccagcgttg acagtgggct tacggcttcg
    11701 aggtgcgtta ctgcattacc gaaacgttca cgcgtggcgc taagctgtgc gcgcaggatt
    11761 tctgctaaac gatattccag ttgctgaatg cgcgtttgcg tgcgatgaat cttcggctgt
    11821 ggattttgct gattcagccg ctgtgttaat cgctgctgct gttgcccggc acgcttaagc
    11881 tgattttcca gcgcaaagct catccgtttt tgcaggcgtt caagcatggt ttgctggcgt
    11941 gccagccgga gctgcggatg ctgctgctgc aagcgatgat gaatctgcgt aaagcgacgt
    12001 gtacggttgg cgagataata atccatcgcc atctccagcc gttgacgggc cgattgcacc
    12061 tggcgcagta actcttgctg attacggctc actacttcag cggcggcaga cggcgttggc
    12121 gcacgcagat cggcaacaaa atcggcaatg gtcacatccg tctcatgccc gacggcgctg
    12181 acgaccggaa tgcggctggc aaaaatcgcc cgcgctacgc gttcgtcgtt aaaactccat
    12241 aaatcttcca gcgaaccgcc gccgcgccca acgatcaaca cgtcgcactc attgcgctga
    12301 ttcgccagtt caatggcgcg aacaatttgc cccggcgcgt catcgccctg aacggaggtg
    12361 gggtagatga tcaccggtag agaaggatcg cgacgtttta acacatgcaa aatatcatgt
    12421 agcgcagcac cggtttttga ggtgatcaca ccaacgcaat gcgcagggga gggaagtggt
    12481 tttttgtatt gctgatcgaa caaacattca gcctgcaact tcgctttgag ctgttcgtac
    12541 ttctgttgca gcagcccttc accggccggc tgcatactct caacgattat ctggtagtcg
    12601 ccgcgcggct cgtagagcgt aatattggcg cgaactaaaa cttgttgccc atgctgcgga
    12661 cggaaggtga cccggcggtt gctgttgcgg aacatcgcgc agcgtacctg ggcggtgtcg
    12721 tctttgagtg taaagtacca gtgaccggaa gccggttgtg tgaaattaga gatttcgccg
    12781 ctgatccaaa cctgtcccat ctcatgctca agcagcagac gaaccgtttg attcaggcga
    12841 ctaacggtaa aaattgcagg ggattgagaa ggtaacatgt gagcgagatc aaattctaaa
    12901 tcagcaggtt attcagtcta tagtaacccg cccttcgggg atagcaagca ttttttgcaa
    12961 aaaggggtag atgcaatcgg ttacgctctg tataatgccg cggcaatatt tattaaccac
    13021 tctggtcgag atattgccca tgctacgtat cgctaaagaa gctctgacgt ttgacgacgt
    13081 tctcctcgtt cctgctcatt ctaccgttct gccgaatact gctgacctca gcacccagct
    13141 gacgaaaact attcgtctga atatccctat gctttccgca gcaatggata ccgtaacgga
    13201 agcgcgcctg gctattgctc tggctcagga aggcggtatc ggctttatcc acaaaaacat
    13261 gtccattgaa cgccaggcag aagaagttcg ccgtgtgaaa aaacacgaat ctggtgtggt
    13321 gactgatccg cagactgttc tgccaaccac gacgctgcgc gaagtgaaag aactgaccga
    13381 gcgtaacggt tttgcgggct atccggtcgt taccgaagaa aacgaactgg tgggtattat
    13441 caccggtcgt gacgtgcgtt ttgttaccga cctgaaccag ccggttagcg tttacatgac
    13501 gccgaaagag cgtctggtca ccgtgcgtga aggtgaagcc cgtgaagtgg tgctggcaaa
    13561 aatgcacgaa aaacgcgttg aaaaagcgct ggtggttgat gacgaattcc acctgatcgg
    13621 catgatcacc gtgaaagact tccagaaagc ggaacgtaaa ccgaacgctt gtaaagacga
    13681 gcaaggccgt ctgcgtgttg gtgctgcagt tggcgcaggt gcgggtaacg aagagcgtgt
    13741 tgacgcgctg gttgccgctg gcgttgacgt tctgctgatc gactcctccc acggtcactc
    13801 agaaggcgtt ttgcagcgta tccgtgaaac ccgtgctaaa tatccggatc tgcaaatcat
    13861 cggcggcaac gtggcaacag ctgcaggtgc acgcgctttg gcagaagctg gttgcagcgc
    13921 ggttaaagtc ggtatcggcc ctggttctat ctgtacgact cgtatcgtta ctggcgtagg
    13981 tgttccgcag atcactgccg ttgctgacgc agttgaagcc ctggaaggca ccggtattcc
    14041 ggttatcgca gatggtggta ttcgcttctc cggcgacatc gccaaagcta tcgccgctgg
    14101 cgcaagcgcg gtgatggtag gttccatgct ggcgggtact gaagaatctc cgggtgaaat
    14161 cgaactctac cagggccgtt cttataaatc ttaccgtggt atgggttccc tgggcgcgat
    14221 gtccaaaggt tcctctgacc gttatttcca gagcgataac gctgccgaca aactggtgcc
    14281 ggaaggtatc gaaggtcgcg tagcttataa aggtcgcctg aaagagatca ttcaccagca
    14341 gatgggcggc ctgcgctcct gtatgggtct gaccggctgt ggtactatcg acgaactgcg
    14401 tactaaagcg gagtttgtac gtatcagcgg tgcgggcatt caggaaagcc acgttcacga
    14461 cgtgaccatt actaaagagt ccccgaacta ccgtctgggc tcctgattct cttcgcccga
    14521 tttcatgtcg ggcgatttat attatctgtt tcacttgcct cggaataagc gtcaatgacg
    14581 gaaaacattc ataagcatcg catcctcatt ctggacttcg gttctcagta cactcaactg
    14641 gttgcgcgcc gcgtgcgtga gctgggtgtt tactgcgaac tgtgggcgtg ggatgtgaca
    14701 gaagcacaaa ttcgtgactt caatccaagc ggaattattc tttccggcgg cccggaaagt
    14761 actactgaag aaaacagtcc gcgtgcgccg cagtatgtct ttgaagcagg cgtaccgata
    14821 ttcggcgttt gctatggcat gcagaccatg gcaatgcagt tgggcggtca cgttgaagcc
    14881 tctaacgaac gtgaatttgg ctacgcgcag gtagaagtcg taaacgacag cgcactggtt
    14941 cgcggtatcg aagatgcgct gaccgcagac ggtaaaccgc tgctcgatgt ctggatgagc
    15001 cacggcgata aagttaccgc tatcccgtcc gacttcgtca ccgtagccag caccgaaagc
    15061 tgcccgtttg ccattatggc taacgaagaa aaacgcttct atggcgtaca gttccacccg
    15121 gaagtgactc acacccgcca gggtatgcgc atgctggagc gttttgtgcg tgatatctgc
    15181 cagtgtgaag ccctgtggac gccagcgaaa attatcgacg atgctgtagc tcgcatccgc
    15241 gagcaggtag gcgacgataa agtcatcctc ggcctctctg gtggtgtgga ttcctccgta
    15301 accgcaatgc tgctgcaccg tgctatcggt aaaaacctga cttgcgtatt cgtcgacaac
    15361 ggcctgctgc gtctcaacga agcagagcag gttctggata tgtttggcga tcactttggt
    15421 cttaacattg ttcacgtacc ggcagaagat cgcttcctgt cagcgctggc tggcgaaaac
    15481 gatccggaag caaaacgtaa aatcatcggg cgcgttttcg ttgaagtatt cgatgaagaa
    15541 gcgctgaaac tggaagacgt gaagtggctg gcgcagggca ccatctaccc tgacgttatc
    15601 gaatctgcgg cttctgcaac cggtaaagca cacgtcatca aatctcacca caacgtgggc
    15661 ggcctgccga aagagatgaa gatgggcctg gttgaaccgc tgaaagagct gttcaaagac
    15721 gaagtgcgta agattggtct ggagctgggc ctgccgtacg acatgctgta ccgtcatccg
    15781 ttcccgggac caggccttgg cgttcgtgtt ctgggtgaag tgaagaaaga gtactgtgac
    15841 ctgctgcgcc gtgctgacgc catcttcatt gaagaactgc gtaaagcgga cctgtacgac
    15901 aaagtcagcc aggcgttcac cgtcttcctg ccagtacgtt ccgttggcgt aatgggcgat
    15961 ggtcgtaagt atgactgggt tgtctctctg cgtgctgtcg aaaccatcga ctttatgacc
    16021 gcacactggg cgcatctgcc gtacgatttc ctcggtcgcg tttccaaccg cattatcaat
    16081 gaagtgaacg gtatttcccg cgtggtgtat gacatcagcg gcaagccgcc agctaccatt
    16141 gagtgggaat gattttgatc cctgcactat gaatgaacaa aaccctccgt tactacagag
    16201 ggttttttat cttcaagaat cataggcttg aagttactaa catcgattaa ttaaaccagc
    16261 tgtccgattt gttctcttct gctttgccca cgcttttcat cagatcgcga ccgccttcag
    16321 tcatatttct gtttgcgtca gcttcagatt gcaccacatc ggtttgcgca gctttgtgct
    16381 tcagttcctg atcgataaat tcgttttcgc gcttaacgcg ggcttcttct ttcgccagcg
    16441 ccagtttttg tttctgaatc tctaagctgc gtagctcatc ttcataactt tgatcgcgtt
    16501 ttttgtccgc agtggcttcg gcgtccagtt tatcctgacg agctttctta tttgccgctg
    16561 ccgttgccgc tcttttgttc gcggcggcct gggcatttgc gcgacgttgc ttctcttgct
    16621 ggatttccct gttgcgctcc gcgacccatt cgtcatgctg cctttgctct tcatttttac
    16681 cttgctgttc cgcttctgcg acagcagaga gttgatcctg caatgatgag gctatagccg
    16741 gatagcttaa ggaggctaag atggcgcaaa gaaaaacttt cttcatgact cctcctgatt
    16801 attagctctt ttcaggacat ttggtatttg gctgaatacg cgtttcgtta tacgttgtgg
    16861 taataacaac ggctaaacct gtcgtaaact ggcactcttt accctcctgg gtggaggtat
    16921 acactttggt gccttcctta tatgttaaag aaacaccttc cactaaggtt ttatcattca
    16981 ccatagaacc cgctgccgcg cctacagctc cgccgccaac tgcccctgcc gtcgttccgg
    17041 aattgctgcc agaaccgacg ttgtggccga taacaccgcc agcgactgcg ccaataagcg
    17101 cgccgaaggc ttgtgcgttc cgtttatttt gggagttgtc tacggcaact tttgcgggaa
    17161 gaatggaaat aatattaacg gttttagttt cttgtttggt attcagttga tcggtttgat
    17221 aaacatcggc ggcatgatcg tcagcatttg actggcatcc tgccagagtg aatgacgcta
    17281 acattgccac aggcagaaga cattttttaa atttcatcac tattccttgt t
//
LOCUS       DAZSIO010000081        17144 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZSIO010000081 DAZSIO010000000
VERSION     DAZSIO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.081"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..510
                     /locus_tag="ACHX5T_002419"
     CDS             <1..510
                     /locus_tag="ACHX5T_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR8467585.1"
                     /translation="PFREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDG
                     FTQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATI
                     RHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADR
                     QYSRAWTVK"
     gene            complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="ACHX5T_002420"
     CDS             complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="ACHX5T_002420"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR8467586.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1780..2868
                     /gene="hcxA"
                     /locus_tag="ACHX5T_002421"
     CDS             1780..2868
                     /gene="hcxA"
                     /locus_tag="ACHX5T_002421"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR8467587.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2878..3075)
                     /locus_tag="ACHX5T_002422"
     CDS             complement(2878..3075)
                     /locus_tag="ACHX5T_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR8467588.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="ACHX5T_002423"
     CDS             complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="ACHX5T_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR8467589.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5543..5956)
                     /gene="entH"
                     /locus_tag="ACHX5T_002424"
     CDS             complement(5543..5956)
                     /gene="entH"
                     /locus_tag="ACHX5T_002424"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR8467590.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5959..6705)
                     /gene="entA"
                     /locus_tag="ACHX5T_002425"
     CDS             complement(5959..6705)
                     /gene="entA"
                     /locus_tag="ACHX5T_002425"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR8467591.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6705..7562)
                     /gene="entB"
                     /locus_tag="ACHX5T_002426"
     CDS             complement(6705..7562)
                     /gene="entB"
                     /locus_tag="ACHX5T_002426"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR8467592.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7576..9186)
                     /gene="entE"
                     /locus_tag="ACHX5T_002427"
     CDS             complement(7576..9186)
                     /gene="entE"
                     /locus_tag="ACHX5T_002427"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR8467593.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9196..10371)
                     /gene="entC"
                     /locus_tag="ACHX5T_002428"
                     /pseudo
     CDS             complement(9196..10371)
                     /gene="entC"
                     /locus_tag="ACHX5T_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10663..11619
                     /gene="fepB"
                     /locus_tag="ACHX5T_002429"
     CDS             10663..11619
                     /gene="fepB"
                     /locus_tag="ACHX5T_002429"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR8467594.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11623..12855)
                     /gene="entS"
                     /locus_tag="ACHX5T_002430"
     CDS             complement(11623..12855)
                     /gene="entS"
                     /locus_tag="ACHX5T_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR8467595.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12966..13970
                     /gene="fepD"
                     /locus_tag="ACHX5T_002431"
     CDS             12966..13970
                     /gene="fepD"
                     /locus_tag="ACHX5T_002431"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR8467596.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13967..14959
                     /gene="fepG"
                     /locus_tag="ACHX5T_002432"
     CDS             13967..14959
                     /gene="fepG"
                     /locus_tag="ACHX5T_002432"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR8467597.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14956..15771
                     /gene="fepC"
                     /locus_tag="ACHX5T_002433"
     CDS             14956..15771
                     /gene="fepC"
                     /locus_tag="ACHX5T_002433"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR8467598.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15768..16901)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHX5T_002434"
                     /pseudo
     CDS             complement(15768..16901)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHX5T_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
BASE COUNT         4007 a         4874 c         4453 g         3810 t
ORIGIN      
        1 ccctttcgtg aaaagccagt ggattgcgtt ctgtgggtta aaaacagcca acttatgcct
       61 aatgattaca acccaaacaa cgtggcacca cccgaaaaaa aactgctgca aaaatccatt
      121 gaaatagatg gttttaccca acccattgtg gtcacacata cggataaaaa tgccatggaa
      181 atcgtggatg gttttcaccg ccatgaaatt ggtaaaggct catcatcatt aaaattacgc
      241 ttaaaaggtt atttgccggt gacctgtctg gaaggtacgc gtaatcaacg catcgccgcg
      301 actattcgtc ataatcgcgc tcgtggacgc catcaaatca ccgccatgtc ggaaatcgtc
      361 cgtgaactta gccagttggg atgggatgat aataaaattg gcaaagagct gggaatggac
      421 agcgacgaag tgctgcgact gaagcaaatt aacggcctgc aagagttgtt tgccgaccgt
      481 caatattccc gcgcctggac ggttaaatag ctaaagctgg cgcaggcgtt cagctgctgc
      541 cagcaacgtc gattccttct tggcaaaaca gagacgaatc agtttatgtg ggaagggatc
      601 ggcgcaaaac accgacagcg gaatcgccgc tacgccgtgc tcctgcgtca gccactggca
      661 aaactcaaca tcatccaggg tagaaaccgc gctgtaatcc accagcaaaa agtatgtacc
      721 ttcacacggt aaaatctcca gccggctttc atttaaagca ttcaccagaa tatcgcgctt
      781 ctggcgataa aagtccggta acgcaagata atgctcaggt tctgcacgta gcatatcagc
      841 aagcgccagc tgtgccgggg tattcaccga aaaggtcaga tactgatgta ccttgcgaat
      901 ttcggcgctg atgggcgctg gcgcaacaca ataacccact ttccagccgg tcatatgata
      961 ggtcttgcca aatgaagaaa ccgccactgc ccgctcacgc agctgcggat gcgccagcac
     1021 actggcatgg ccctgttgtg aaaagttgat gtgctcgtag acttcatcgc taatgacaaa
     1081 aatctcgtgc ccggcgatcg cctgccacaa agcggcgaaa tcagcctgct gccagacagt
     1141 tgcactgggg ttatgcggag tgttgaggat caccagtctg gtgcgctcgc ttaataatgc
     1201 ggcaaattcc tgccagtcaa cgcgaaaatg cggtggttgc agtgccatac gtttcactat
     1261 tcccccagaa agcgcgatgg cgggggcgta actgtcatag ctgggatcaa aacaaatcac
     1321 ttcatcgcca ttgcgcacca gtgcggtaat cgccgcgtat aacgcttccg tcgcccctgc
     1381 cgttacggtg atatcgctat cggcatctgg ttgatagcca tacaaacgtt ccgttttctg
     1441 agcaatcgcc tcgcgcaagg cctgcacgcc ggtcatgggc gcgtattggt ttgccccctg
     1501 ggcaacgtgg tacgccagcc gctcctgtaa atagcgcgga ccatcaaaat caggaaagcc
     1561 ttgcgacagg ttaatcgcct ggtgttgctg cgccagcgcg ctcatctggg tgaaaatagt
     1621 ggtgccaagt tgtggaagtt tgctttgtgg aatcagaggg ttatttgtca tttttattgt
     1681 acctgacgaa gttgcgtgtt gaaggcacta taacacgatg ccagtatttg gcaatcaaga
     1741 cgtttagatg tctaaataaa acaataagga caacacaaca tgcctcacaa tcctatccgc
     1801 gtggtcgtcg gcccggctaa ctacttttca catccaggaa gtttcaatca cctgcacaat
     1861 tttttcactg atgaacaact ttctcgcgcg gtgtggatct acggcaaacg cgccattgct
     1921 gcggcgcaaa ccaaacttcc gccagcgttt ggacttccag gggcaaagca tattttgttt
     1981 cgtggtcatt gcagcgaaag cgatgtacag caactggcgg ctgagtccgg tgacgatcgc
     2041 agcgtggtga ttggcgtcgg cggcggcgca ctgctcgaca ccgcgaaagc cctcgcccgc
     2101 cgtctcggtc tgccatttgt tgccgctccg acgatcgccg ccacctgcgc agcctggaca
     2161 ccgctctccg tctggtataa cgatgccgga caggcgctgc attatgagat tttcgacgac
     2221 gccaatttta tggtgctggt ggaaccggag attatcctca atgcaccgca acaatatctg
     2281 ctggcgggga tcggtgacac gctggcgaaa tggtatgaag cggtggtgct ggctccgcaa
     2341 ccagaaacgt tgccgctaac cgtgcggctg gggatcaata atgcgcaggc cattcgcgac
     2401 gtcttgttaa acagtagcga acaggcactt gccgatcagc aaaatcagca gttaacgcaa
     2461 tcattttgcg atgtggtgga tgctattatt gctggtggtg ggatggtcgg tggtctgggc
     2521 gatcgtttta cgcgtgtggc ggcggctcat gccgtgcata acggtctgac tgtcctgccg
     2581 caaaccgaga agtttcttca cggcaccaaa gtcgcttacg gaattctggt gcaaagcgcc
     2641 ttgctgggtc aggatgatgt gctggcgcaa ttaaccgggg cgtatcagcg ttttcatctg
     2701 ccgactacgc tggcggagct ggaggtggat atcaataatc aggcggagat cgacaaagtg
     2761 attgcccaca ccttgcgccc ggtggagtcc attcattacc tgccagtcac gctgacacca
     2821 gatacgttgc gtgcagcgtt cgaaaaagtg gaatcgttta aagcctgacg tgcaaattta
     2881 gcagcagcgc gcgccgcctt ttccaccgta gcgcgcgtcc tgccgctcac ggaaaaactc
     2941 ctcgtaggtc atcggcgttt gatcgggatg attaacccgc atatgttcga catagttgtc
     3001 gtaatcaggc ataccaatca tcagcttcgc cgcctgacct aaatattttc cggcttttgc
     3061 cagtgaatca aacatcttgg cctccgctaa ccatgttgct gaaagagtgc cggatgcggc
     3121 gtgaacgctt tatccggcct acaaatgcta tcaacgcact cggtcagtcc cctcgcccca
     3181 tcggggagag ggttagggtg agggggaacc ctcggcacta tgccaacatt cgccaacatc
     3241 ccccctcact ctgactttag tgcgcgcctt tcgcctgcgc tacgatctcc tcgacatttt
     3301 ccggcatcgg ctcatacggc gtttctttcg ccgttggctt cggatctttc aatgccgcaa
     3361 gtgccgtctt aatcgagaac agtgccagga ccaccacgac caccataaag aagatggtta
     3421 acccggcatc cagacggttg ttgaacacca gttgcgccag ctgtgactca gtatactgcg
     3481 acggaatatt gccgctgtcg atcattgcct ggaacttgtt agcaatgacc aggaagccga
     3541 ctttcgcatc cgggctaaac gctttcagcc agtcagcggt cagggtacaa atcagcagcc
     3601 aggccgttgg taccagcgcc acccaggcgt aacgttgacg tttcatcttg aacaacacca
     3661 cggcacagag catcagcgcc atccctgcca gcatctggtt ggcaatacca aacagcggcc
     3721 acagagtgtt aataccgccc agcggatcga ccacgctctg atggaggaag tagccccacg
     3781 ccagtacgca cagcgctgtt gccagcaggt tagcaggcag tgaatcggtc cgcttcaggc
     3841 caggagacac cacgcccagc agatcctgca acataaagcg cgcagcacgc gtacccgcat
     3901 ctaccgccgt cagaataaac agagcttcaa acagaatggc gaagtgatac cagaacgcca
     3961 catccatcat accacccagc gcaccgtgca ggatgtaagc catccccacc gccaacgtcg
     4021 gcgcaccgcc cgcacgggaa atgatcgact gttcacccac ttcgctggca atctggttga
     4081 gcgtatctgg tgtaatgcta aagccccagc tactcaccac ctgcgcggca gaagcgacta
     4141 catccgccgt ccctgccgga gccagcaccg ccattgggct gttcatggca aaatacacgc
     4201 ccggatcgat gatacaggca gaaactagcg ccataatcgc cacgaaggat tccatcaaca
     4261 tcccaccgta gccgataaag cacgcctgcc cttcgttcgc cagcatcttc ggcgtggtgc
     4321 cggaagagat cagcgcatgg aagccagaca ccgcaccaca ggcgatggtg ataaacagga
     4381 acgggaacag gttaccggtc cataccgggc cagtgccatc gacaaatttg gtcagcgcag
     4441 gcatggtcag cgtcgggcgc ataatcaaaa tgcctaccgc cagaccgacg atagtcccga
     4501 ttttcaggaa ggtcgagaga tagtcacgcg gggccagcag caaccacacc ggcagcaccg
     4561 ccgccacaaa accgtagccc accagcatcc aggtcagctg cacgccggta aagtcaaagt
     4621 acggtgccca ggtcggactt tctgccaccc agccgccaga gataatggcg aaaatcagga
     4681 acaccaggcc aatcactgac acttcgccaa tgcggcccgg acgcagatag cgcaggtaga
     4741 ttcccataaa aatcgccagc ggaatggtga acgcaacggt atacgttccc cacgggctat
     4801 gagtcagggc tttcaccacg atcatcgcca gtactgcaag gataatgacc atgatcataa
     4861 agcaggctac cagcgcaatc accccggcag ttggccccat ctcttctttg accagctcac
     4921 ccagcgagcg accgtcacgg cgcgtagaaa caaacagcac catgaaatcc tgcaccgcac
     4981 cggcgagaac caccccggcg agcagccaga tcatccccgg caggtagccc atttgcgctg
     5041 ccagcaccgg ccccaccagc ggccctgctc cggcaatggc cgcaaaatgg tgaccgaaca
     5101 gcactttctt gtccgtcggc acatagtcca gcccgtcgtt atggcgcacc gctggcgtca
     5161 tacgcgtcgg gtcaaccgcc agcacatttt tggcgatata cagcccataa aaacggtaag
     5221 cgatcagata gatacagacc gacgccacca caatccacag cgcgttgatc tgttccccac
     5281 gatttaaagc aatgtatccc agagcaaatg ctcccattac agagagcact gtccagacga
     5341 ggtatttccc tgatttgttc atagttgtta tccgtgtgcg tgtccataga gatgttacat
     5401 tttgtttcta taacatcttt gtacgattta acaacacggt aaccatgtaa atcggagttg
     5461 aaaccaatat ttaacttaac aatgttaact cgatcactcc gttaaccgag ttttcctgca
     5521 atctcattta attctgtcgg gctcatccca aaactgccgt acccagccga caagtgcagc
     5581 aacgccgccc ctgttcatcg aaaacgacga tttcccaact ctgattttgc ctcccaagat
     5641 gcagcggctg gcagacgccg cgtaccttgc cttcagacac cgggcgatga tgggtggcgt
     5701 taagttccgt gcccaccaca cactgcccgt cacgggtcat cataaatccg gccatcgatc
     5761 ccagcgtttc agccagcgcc gccgacgcgc cgccatgcag caggccaaac ggctgatgag
     5821 tacgggtatc aaccggcatt tcggcttcca gcacatcatc tcccagaagg gtatacacaa
     5881 ttcccagatg cgccaccatt gtgttatcgc tggtggcgtt cagttcgtcg agcgttaaat
     5941 ggcgtttcca gatcatgctt atgcccccag cgttgagccg ccatcgacca caatatcctg
     6001 tagggtgata tggctggcga ggtcagaggc gaggaacaaa atcgtgttgg cgatctcttg
     6061 tggacgggcg attttcccca gcggaatgcc gagtttaaac tgctcgccaa agccgcgaat
     6121 acgctgttct tcggcgtcat cgctcaccca cagcgtgcgt tgcatatcgg tgtcggtgga
     6181 gccaggcgaa accacattac agcgcacgcc gctacccgcc agttccagcc cgacgctcaa
     6241 cgccaggctt ttcagcgccg ctttcgatgc gccataagca ctcataccaa tacgcgccgt
     6301 gtgtgcggcg tcggacgcca cagtgacaat cgccccgccc cgctgacggc gaaactggtt
     6361 catggtttgc tggaacaggt taaacgcacc gccgacgtta accgcaaaag tctgctgcca
     6421 gtcctcttta ctgagctgat cggtcgcgcc catgcgcaaa attcccgccg cattgatcag
     6481 cacgtccagt cgctccgttt cagctaacag tcgctgacat acctgcgcca cctgcccggc
     6541 gtcggcaaca tccattactt cggtcgcaaa gggatattgc tcctgagtga acgcttgatc
     6601 aaaacctgta actttcgctc ccgcctcaac aaacgtcagc gccgtggcgt agccgatacc
     6661 tttacctgcg ccggttaccc agacattttt accgctgaaa tccattattt cacctcgcgg
     6721 gagagtagct tccaccaggc gtcgatggtc gggtttttcg ccagcatgac aaagtcgata
     6781 tcaccatgca ctttgcgcca gcgcgccgcc agcgccatca tgcgcaccga atccagaccg
     6841 tagtcgatca ggttgtcgtc atcgaacggt tcatcggact cgtccagcaa cggcaggatc
     6901 acctcacgca gcgccgcttt gctggcgggg ataggtgctg gcagtaattc ttcggtcatc
     6961 accacccggc cagaacgtcc ggccacatat ttcagcgaca tcaaatgctc gtcacggctg
     7021 aaatcagcca gcgcatccgc caccataaac ggtttaatat cgcgcataaa tgcgtcggtt
     7081 gcggtggtca tacagccaat gtgggcatat actcctgtaa taatcagctg gttacgtcca
     7141 ctctctttga gcatttgctc cagcggagaa cgatgaaacg cgctgtagcg ccacttcacc
     7201 agcaccgtgt cgtcggcatc tggcgtcagg cgatccacca ccttttgctg ttccggcgag
     7261 cgggtcaggc ccggccccca catatcattc aacagcgccc gatcttcatc gctctgctct
     7321 ttcggctggg cggtgtaata aaccgggata ttgtgctgtt tgcagtagtc gcgcagcgca
     7381 gcaatattcg cgatcacctg ctccatcatc gggcagttct cgccccagaa gctgacaaaa
     7441 tagtcctgca tatcatggat taacaacgcg gcacgttgcg gttcaaaggc ccagtcaact
     7501 ttattctgcg gaatatcgtg agactccggc agtgcgtaag cctgtaattt tggaatagcc
     7561 atcgtgttct ctccttcagg ctgatgcgcg tgacgccagc cactgacgta attgtttttt
     7621 atcgactttc ccgaccgccg tcagcggaag tgaatccaca cactccacgc gatccggtaa
     7681 tttaaattcg gcaataccct gttcacgcag gaaacgacgc acctgcaccg cgcgcagcgg
     7741 ctcttttacc accagataag cacagctttt ttcgcccatc agctcatttt ccatgctcac
     7801 cagtgcggca tagatcactg ccggatggct cagcagcagg ttttcgatat cttcggcagc
     7861 gatcttctcg ccgccacggt tgatctgatc tttctcgcgc ccctgcacgg tgatgtaacc
     7921 ctctggatca atagagatca gatcgccgga acagtaaaaa ccgttggcat caaaggcact
     7981 ggcattgtgc tgcggacttt tgtaatagcc acggaaggtg tacggcccgc gcgtcatcag
     8041 gcgtccaact tccccttgcg gcagtggatt tccttcggca tcggcaaccc acacttcatc
     8101 gtccggacac attgggtaac cctgggtatg gataattttc tccgcgctat catcaagacg
     8161 ggtgtagttc accagccctt ccgccatgcc aaacacctgc tgcaactggc agccaatctc
     8221 agcgggaata cgcgctgcaa gcgtggcaga aagacgtgcg ccgccgacct gtaacagttt
     8281 cagcgaggca agctgcgccc ggctttcgcc ttcagccagc gcctgcaacc acaggctgac
     8341 tgccggcggc accagcgcgg taacgttaac ctgatgtttt gcaatcaacg ggaagcaaag
     8401 cgtggcgctg ggatcggccg ccagaacaac cgttcctccg gcaagaaaga cgcccagcga
     8461 tcctggcgaa ctcatagtgt agttatgagc cgccgggatc gagcacaggt agcgcgtctg
     8521 ttgtgtgaac tgacaaatct cggcgctacg acgcacgctg tagtaatagt cgttatgagt
     8581 gcgcgggatc agtttcggtg tgccggtggt gccgccggaa agctggaaat aggccacttc
     8641 atcagcaggt gatggcgtgg cagtaaaacc ctctgccgga tggttaatcg catcctgcaa
     8701 gttatgctca ccgctgtcgt tgtgcagttg caccacgcga atggaagaat gctctgcaac
     8761 aaatgtgttg aggaaatcat ccccgctaaa tagcgcatgc tggcgatcgg caatcagtaa
     8821 cgccggttca atctggctgg cgtaggcgtt cagttcacta cgctgatggc tgaacagcgc
     8881 cagcaccggc gcgacgccca gtttcagcag cgcgaaaaag gtaatgtaaa gttcagcgac
     8941 gttacccagt tgtaccagcg cggtttcacc aggtttaatg ccctgacggc gcaaactaca
     9001 ggcgaggtta tccgccgcct gattcagctc ccgataactc aactgccgct cgccgtcgat
     9061 aaccgcgatg ctgtcactcg cagcgtggcg agtcagaatg tcggtcagtg gcaaatcctg
     9121 ccagtagcct ttttcccgat agcgacgggc aaactcttcc ggccagcggg tgaatggaat
     9181 gctcatcctc gctccttaat gcaatccaaa aacgttcaac atggtagaaa gtttgacgcc
     9241 tgtttcgcgc cactcaccca acggtgacga cgcaggcaca atccccgctc cggcaaacag
     9301 acgcacctga ttttcccgca gcttcgcgca gcggatggtc accacccatt cgccgttacc
     9361 ttcgctgtca caccaaccca caatgccgcc aaacagttcg cggtcgaacg gctccagttc
     9421 agcaataacc tgggtcgcgg cctgatgcgg gaaaccgctc agcgcggggg tcggatgcag
     9481 cagacaggcc agagtcagtg cgttttcttg cgaattcgct ttaccttcaa agggagttgc
     9541 gagatgccac agcgtcggcg tggtaatcaa ttgtggagag gagggaacgt gtaactcact
     9601 actgcgttcg cgcagtacct ctttcatcgc ctgagtcccc agttcatgtt catggcgatc
     9661 tttttctgac gccagcagac gattacccgc ttcgcgatcg agcacttcat ccggctgacg
     9721 acgcgcggaa ccggctaacg gaatggagct aaaacgctcg ccgtctttac gtagcagcag
     9781 ttccgggctg gcccccagca ggacgccacc atcagccagc gggacatgga agttgtaact
     9841 aaccgggttt tgcgcaatca accgttccag caatacgcca ctatcaatgg cggcgtcagt
     9901 ggtgatatca atcaatcgtg acaacaccac tttgtcgacc tgcggcgtgg cggtaagtgc
     9961 ggcggcgcgg gcacccatct gttcaaacgt ggtttgttca ggaattgcct agcgttccac
    10021 cacgttcagc gactggctgc gggtaaaaca gcgggctgag gtctgttttt cctgacggga
    10081 gaacgactgc taggattcgg gaatatacag cgacgaaggc tgacgtggat cgaagggaat
    10141 cgccccgacc atcaccggat ttttgatgcc ctgcgctttg gcatcggcaa acagcgcggc
    10201 gagtttttgc tggaagggac tgtcgggcga atccccgttc acagccggtt catcgaaacg
    10261 ggcgaaacat cctgacgtcg taaaactgcg gtacggcgac ataaagaaaa agcgattggg
    10321 cgcaagtgtt gccatggtct gctgtacttc ctcagccagt gacgtatcca tatcatcctc
    10381 cacaaaatga taaaggcttt aataatgatt atcacttata ttttcggccg ctaacctaaa
    10441 agcaaacgcg cactatgtca actcttgagg taacgcaatc tgcaactcgg ggttgcatct
    10501 ggttgtggtt acaatgaaaa tgagaagcat tattgatgaa ttcgcataag cgcaatgtga
    10561 tgtcctgcgc cgttttgccc cctctcccca aagggacgga ttgtgcgctt tgtcgaattt
    10621 gtcattacgc ccttaacctt attaataaca ggaagctgac ttgtgagact cgccccgctc
    10681 taccgcaacg cccttctatt aacaggactt ttgctttcag gaatagccgt agttcaggcc
    10741 gccgactggc cgcgtcagat tactgacagc cggggcactc ataccctgga aagccagccg
    10801 cagcgtattg tttccaccag cgtcaccctg accggatcac tgctggcgat tgatgctccg
    10861 gtgatcgcca gcggcgcgac cacgccgaat aaccgcgtcg cagatgacca gggcttttta
    10921 cgccagtgga gcaaggtggc gaaagaacgc aaactgcaac ggctctatat cggcgaaccg
    10981 agcgccgaag ccgttgccgc gcaaatgccg gatctgattt taattagcgc aaccggcggg
    11041 gattcggcgc tggcgctgta tgatcagctt tccaccatcg ccccgacatt aatcatcaat
    11101 tacgacgaca aaagctggca gtcgctgtta acgcagcttg gcgaaattac cgggcatgag
    11161 aaacaagcgg cagagcggat tgcacagttt gataagcaac tggcagcggc gaaagagcaa
    11221 atcaaattac cgccgcagcc ggtcactgcc attgtctata ccgccgctgc acacagtgcc
    11281 aatctctgga cgccggaatc agcacaaggg cagatgctgg aacaactcgg ctttacgctg
    11341 gcgaaattgc ccgcagggtt aaacgccagc caaagccagg gcaaacgcca tgacatcatt
    11401 cagcttggtg gggaaaatct ggctgcgggg ttaaacggtg aatcgctatt cctgttcgct
    11461 ggtgatcaga aagatgccga tgcgatttat gccaatccac tgctcgcgca cctgcctgcg
    11521 gtacaaaaca agcaggttta tgcgctggga accgagacgt tccgcctgga ttactacagc
    11581 gccatgcaag tgctggaacg gcttaaggcg ctgttttaag cattaactgt cggacgctgt
    11641 cacctgcggc ggcgtctggc gaaaacgtcg caactccacc agcaccagca ataacaacac
    11701 gccgataatc aacaaaccaa aaccgctcgc gcttgcggaa gcaaccggtg tcatcatcgc
    11761 gcccaaacca cccagcagcg ccgcgcctat ggcatcgccc gtcacgttct gcgccgtcca
    11821 caaaccgtta atccgcccta acatcgcttc cggggtttgc gtttgcagca ttgtatattg
    11881 cagcaacgaa ctaaccgcgc tcagccagcc gaacagcgcc agacaaacca cgcctaaaat
    11941 ccacatcggc atcaggccaa acagaccaat ggcgaggaac gatcccagcg tggagagcag
    12001 catcaataac cctggtcgcg cactatgtgc cagcttcccg ctggttaacg caccaatagc
    12061 cgcgccgagc gggatcgccg cgtagagaaa accaatctgt gccgctgaca tctgccagtt
    12121 gtcagccagc gccggataca gtaccagcac cgcgctcgcc atcgtcaata aaccacccag
    12181 cagcgggctg gcaagcagaa aacgaaatcc tgccagtaat gatttcaacg gatgctcaag
    12241 cggctgcggt ggcggtggca actccggaag gcttaataac ggtaacaagg taataaacgt
    12301 gcccgccgcc gccagcccgt agttccaggc tacgccgccg gtcgccagca ataaaccgcc
    12361 aatcatgggc gaaatcaccg accccaggcg cacggtcaac atggtgatcg ccccggcctg
    12421 cattaagttt tcacgcccta ccagtgcaga tgttgccgcc agtagcgccg taacaccaag
    12481 tgatgcgaaa aaaccatccc ataaaccaag taaatagatt gccagcaatg acggctccgg
    12541 cagcagtgca ttaaggcaca gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa
    12601 aatcactttt ttgcgttcat agcgatccgc cagcacaccg ccaaccatca ggccaacaaa
    12661 catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc caggtagaat gggtcatcat
    12721 ctggatctgc accggcaccg cgacgccgag caaacccaga gacacaattg agataaaacg
    12781 agcgaggaat actgcgcgaa acgccgggtg cgttttcaac aggctgaggt taagcagcca
    12841 ggattgttta ttcattacaa tgccttgcca tcaaatattt taaataccta ttcttaggct
    12901 gcacatgtta acatatccaa ataagatcga taacgataat taatttcatt atcatggaag
    12961 ttcgtatatc tggttctgtt gccgtgacac gcgccattgc cgtgcccgga ttgctgttat
    13021 tactgattat cgcgacggca ttaagcctgc tcattggggc aaaatcactc cccgcttccg
    13081 tagtgctgga ggccttctcc ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg
    13141 cgcggctgcc gcgtaccctt gccggtttac tggcaggcgg cgcgcttggc cttgccgggg
    13201 cgttaatgca aaccctcacc cgaaacccac ttgccgaccc cggcttgctt ggcgtgaacg
    13261 ccggagccag ctttgccatt gtgctgggtg cggcgctgtt tggttactca tccgcacagg
    13321 aacaactggc gatggccttc gccggggcgc tggtggcctc attgattgtt gcctttaccg
    13381 gcagtcaggg tggagggcag ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg
    13441 cggcggtgct ggaaggactg accagcggca tcgccctgct taatcctgac gtctacgatc
    13501 aactgcgttt ctggcaagcc ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg
    13561 tgctgatccc ggttctgatc gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca
    13621 gtttgagcct cggcagtgac accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt
    13681 tgattggtct gctggcgatt accgtgcttt gtggtagtgc gacggcggta gttggcccga
    13741 ttgcctttat cggcctgatg atgccgcaca tggcgcgctg gctggtaggt gccgatcatc
    13801 gctggtcgct gcccgtcacg ctacttgcta cccctgccct gctgctgttt gccgatatca
    13861 tcgggcgggt gattgtgccc ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg
    13921 caccggtgct gatcttcctc gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc
    13981 tcgccgatta ctcatcacct gtttgctgct gatttccgcc tgtgtggttg caggtatctg
    14041 gggattacgc agcggtgccg tcacgctgga aatctcgcag gtattcgccg cgttgatggg
    14101 cgatgcgccg cgcagtatga cgatggtggt caccgaatgg cgtttaccac gcgtgctgat
    14161 ggcgctgttg attggcgcgg cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg
    14221 taacccgctc ggcagccctg acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct
    14281 ggtggcgatg gtgctgtttg gtcaggatct gacggctatc gcgctggcag caatggtggg
    14341 cggcattatc acttcgctgt tggtctggct gctcgcctgg cgtaacggta tcgacacctt
    14401 tcggttgatt attatcggta tcggcgttcg cgccatgctg gtggccttta atacctggct
    14461 gttgctgaaa gcgtctttag aaacggcgct aacagcaggt ttgtggaatg ccggatcgct
    14521 caacggcctg acgtgggcaa aaacctcgcc ttccgcaccc atcattatat tgatgctcat
    14581 tgccgccacc ttgctggtgc ggcgaatgcg cttgctggag atgggcgatg ataccgcctg
    14641 cgcgctgggc gtcagcgtcg agcgttcgcg tctgttaatg atgctggttg cagtggtgct
    14701 taccgctgcg gcaacggcgc ttgccgggcc gatttccttt attgctttag tcgcgccgca
    14761 cattgcccga cgcattagcg gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg
    14821 agcgctgtta ctgctggcgg ccgttctctg cgcccaacaa ctgtttatgc cgtatcaact
    14881 tccggttggc gtcgttaccg tcagcctcgg cggtatttac cttatcgtct tgttaattca
    14941 ggagtctcgc aaaaaatgac cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg
    15001 ggatatggca aatataccgt tgcggaaaat ctgactgtag aaattcctga tggtcacttc
    15061 acggcaatta tcggaccaaa tggctgtggt aaatccacgt tactgcgtac cttaagccgc
    15121 ctgatgacgc ctgctcacgg gcatgtctgg ctggatggcg agcacattca acattacgcc
    15181 agtaaagaag ttgcacgccg gattggtctg ttggcgcaaa atgctaccac gccgggcgat
    15241 atcaccgtgc aggagctggt ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc
    15301 tggcgcaaag aggatgaaga agcggtaacg aaagcgatgc aggccacggg aataactcat
    15361 ctggcagatc aaagcgtgga taccctttct ggcggacaac gccagcgagc gtggatcgcg
    15421 atggtgctgg cccaggaaac ggcaattatg ctgctcgacg aacccacgac ctggctggat
    15481 atcagtcatc agattgatct actggaactg ttaagcgaac tgaaccgcga gaaaggctat
    15541 accctggcgg cggtgctgca cgatcttaat caggcctgtc gttacgccag ccatttgatt
    15601 gcattgcggg aagggaaaat tgttgctcag ggagcgccga aggagattgt cactgctgaa
    15661 ctgattgagc gcatttatgg tctgcgatgc atgatcattg acgatccggt agccggaatg
    15721 ccgcttgtgg tgccgctcgg acgaacggca ccttcaaccg caaatagtta aactaagtgg
    15781 tctgccatca tggcatcctg ttttctggat gccatcgcat agcgcaataa cacgctacca
    15841 caagccacca tcccgccgat cagcgcggaa aggatcacaa taatcgcctt acccggaccg
    15901 tcttttttca ctggcaatga cgggcttaac tgatatttaa acggcgtaaa attcacatcg
    15961 ttgacatttg cttttgttaa ttgctcgaca agatactgcc gattacgtaa ttcaccgttc
    16021 agttccgcaa cgtcagtaac cgctttttca atttccagtt tgcgttcaat accgtctgcg
    16081 ccgagggaaa tagagaaatc ggggtcatct ttaacggcct gaccattact gtatacgggc
    16141 tttttaattc ctgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca
    16201 tcaagttgat ttttcgtttt aatgcgatcc tgagccagtt tttctttttc aaactgggtt
    16261 ttgatctcca gtttattacg gacgttttct atcgactctt tcaccaccaa cgcagagata
    16321 taatcgatat acccgctcaa aacggtctgc gcctctttac tggttggcgc ggtaaaactt
    16381 agcgtccagg atgtatacag tgacggttca tcttttttct tactagcatt gtcatcaacc
    16441 gctttcattt tttcgctcaa tgcaacaata gcgcgatgca aatccagttc atcgattttc
    16501 gcctctttta attagtccat cacataaggt gatgaacgca ggtactcttc cagcaagcta
    16561 acagactgaa acttcttgat aaacaggtta aatgcaccgc cacggtcgat attaacatcg
    16621 agatcaagaa cgcggagttt ggtaaaggtt ttctccaggt cctgccactg aatggcttcg
    16681 gcgggagtaa taacggctga gctggtccat ttttgcggca ggatgaaaga gatcagtaaa
    16741 cctgcgcagg caaatgcaaa aacgaccgcc atgaccgttt ttttggcccg ccataaaact
    16801 tcgattagat taagtaaatc aatttcatta ttactgggcg acgccagagg atactcagaa
    16861 aaatgagcgt cacttccctg tttaatattc aatgatgaca tggggtataa cctgaaaatg
    16921 agtccaatag gggaaaatct ccacggcgct cactttacca gttggtgaga attatccgaa
    16981 gctgaagttt gtaaattcct tccactgaat gcaacattca ctctatttaa gagaaatatt
    17041 catcaaacct ataaatcaaa caaataaaaa acccataatt attttctgta attatgggta
    17101 atgactccaa cttactgata gtgttttatg ttcagataat gccc
//
LOCUS       DAZSIO010000082        17093 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZSIO010000082 DAZSIO010000000
VERSION     DAZSIO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17093
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.082"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            87..1226
                     /gene="arnB"
                     /locus_tag="ACHX5T_002435"
     CDS             87..1226
                     /gene="arnB"
                     /locus_tag="ACHX5T_002435"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR8467599.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1230..2198
                     /gene="arnC"
                     /locus_tag="ACHX5T_002436"
     CDS             1230..2198
                     /gene="arnC"
                     /locus_tag="ACHX5T_002436"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR8467600.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2198..4179
                     /gene="arnA"
                     /locus_tag="ACHX5T_002437"
                     /pseudo
     CDS             2198..4179
                     /gene="arnA"
                     /locus_tag="ACHX5T_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            4176..5066
                     /gene="arnD"
                     /locus_tag="ACHX5T_002438"
     CDS             4176..5066
                     /gene="arnD"
                     /locus_tag="ACHX5T_002438"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR8467601.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVECCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5066..6718
                     /gene="arnT"
                     /locus_tag="ACHX5T_002439"
     CDS             5066..6718
                     /gene="arnT"
                     /locus_tag="ACHX5T_002439"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR8467602.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            6715..7050
                     /gene="arnE"
                     /locus_tag="ACHX5T_002440"
     CDS             6715..7050
                     /gene="arnE"
                     /locus_tag="ACHX5T_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR8467603.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7050..7436
                     /gene="arnF"
                     /locus_tag="ACHX5T_002441"
     CDS             7050..7436
                     /gene="arnF"
                     /locus_tag="ACHX5T_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR8467604.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7430..7702)
                     /gene="pmrD"
                     /locus_tag="ACHX5T_002442"
     CDS             complement(7430..7702)
                     /gene="pmrD"
                     /locus_tag="ACHX5T_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR8467605.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(7807..9162)
                     /gene="menE"
                     /locus_tag="ACHX5T_002443"
     CDS             complement(7807..9162)
                     /gene="menE"
                     /locus_tag="ACHX5T_002443"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR8467606.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9159..10121)
                     /gene="menC"
                     /locus_tag="ACHX5T_002444"
     CDS             complement(9159..10121)
                     /gene="menC"
                     /locus_tag="ACHX5T_002444"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR8467607.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(10121..10978)
                     /gene="menB"
                     /locus_tag="ACHX5T_002445"
     CDS             complement(10121..10978)
                     /gene="menB"
                     /locus_tag="ACHX5T_002445"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR8467608.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10993..11751)
                     /gene="menH"
                     /locus_tag="ACHX5T_002446"
     CDS             complement(10993..11751)
                     /gene="menH"
                     /locus_tag="ACHX5T_002446"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR8467609.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11748..13418)
                     /gene="menD"
                     /locus_tag="ACHX5T_002447"
     CDS             complement(11748..13418)
                     /gene="menD"
                     /locus_tag="ACHX5T_002447"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR8467610.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13507..14802)
                     /gene="menF"
                     /locus_tag="ACHX5T_002448"
     CDS             complement(13507..14802)
                     /gene="menF"
                     /locus_tag="ACHX5T_002448"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR8467611.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLLPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14881..15186)
                     /gene="elaB"
                     /locus_tag="ACHX5T_002449"
     CDS             complement(14881..15186)
                     /gene="elaB"
                     /locus_tag="ACHX5T_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR8467612.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15241..15702)
                     /locus_tag="ACHX5T_002450"
     CDS             complement(15241..15702)
                     /locus_tag="ACHX5T_002450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8467613.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15767..16684
                     /gene="rbn"
                     /locus_tag="ACHX5T_002451"
     CDS             15767..16684
                     /gene="rbn"
                     /locus_tag="ACHX5T_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR8467614.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16875..17063
                     /locus_tag="ACHX5T_002452"
                     /pseudo
     CDS             16875..17063
                     /locus_tag="ACHX5T_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         3984 a         4627 c         4344 g         4138 t
ORIGIN      
        1 aaatttcgct ccaaatggca aaatatacac aacactcttt atagcaaata taagtggaca
       61 ggtattcaat ggcggaagga aaagcaatgt cagaattttt gcctttttcg cgaccagcaa
      121 tgggcgtgga ggaactcgct gcagttaaag aggttctcga atccggttgg atcacaaccg
      181 gtccgaagaa tcaggcgctt gagcaagcct tttgccagtt gacgggaaat cagcatgcca
      241 tcgcggtcag ttcagccacc gccggaatgc atatcacgct aatggcgttg gaaattggca
      301 agggcgatga agtgattacg ccttccctga cctgggtttc aaccctcaat atgatttcct
      361 tgctgggtgc aacgccggta atggtggatg tcgaccgcga tacgctgatg gtcacgcctg
      421 aagcgatcga atcagccatt acgccacgca ctaaagccat cattccggtg cattatgccg
      481 gtgcgccagc agatattgac gccattcgcg ccattggcga acgttacggc atcgcagtta
      541 tcgaagatgc tgcccatgcc gtcggtacgt attacaaagg gcgacatatt ggcgcaaaag
      601 gtaccgctat tttttcattt catgccatta aaaatattac ctgtgctgaa ggtggcctga
      661 ttgtaactga taatgaaaac cttgcccgcc agctacggat gctgaaattt cacggtctgg
      721 gtgtcgatgc ctatgacaga caaacctggg gccgtgcacc gcaggctgaa gtcttaacac
      781 cgggctataa gtacaatctg accgatatta acgccgcgat tgccctgaca cagttagtca
      841 aattagagca cctcaacacc cgtcggcgcg aaattgccca gcaatatcag caagcactgg
      901 cagctctccc ctttcagcca ttaagccttc ccgcctggcc gcacgttcac gcctggcatc
      961 tgtttattat tcgtgtcgat gaacaacgtt gtggtatcag tcgcgatgcg ttgatggaag
     1021 cgttaaaaga aagaggcatt ggtaccgggt tacatttccg cgccgctcac acacaaaaat
     1081 attatcgcga gcgttttccc acgctgtcgt taccgaatac cgaatggaat agcgaacgca
     1141 tctgttcgtt gccgctgttc ccggatatga ctaccgccga tgccgaccgc gtcatcacag
     1201 cccttcagca actcgcagga caataagcca tgtttgaaat ccaccctgtt aagaaagtct
     1261 cggtggttat tcccgtttat aacgagcagg aaagcttacc ggaattaatc aggcgcacca
     1321 ccacagcctg tgaatcgttg gggaaagagt atgagatcct gctgattgat gacggcagta
     1381 gcgataattc cgcgcatatg ctggtcgaag cctcacaagc ggagaacagc catattgtgt
     1441 ctattttgat taaccgcaat tacgggcaac attcagcgat tatggcggga ttcagtcacg
     1501 ttactggcga cttaattatt acccttgatg ccgatctcca gaatccgcca gaagaaatcc
     1561 cccgcctggt ggcaaaagcc gatgaaggtt acgacgtggt agggactgta cgccagaacc
     1621 gccaggacag ctggtttcgt aaaacagctt cgaagatgat taaccggctt attcagcgca
     1681 ccactggcaa agcgatgggt gactacggtt gtatgctgcg cgcctatcgc cgtcatattg
     1741 tcgatgcgat gttgcactgc catgaacgca gcacctttat cccgattctg gcgaatatct
     1801 tcgcccgccg tgcaattgaa attccagtac atcatgccga gcgtgagttt ggtgaatcca
     1861 aatacagttt tatgcgcctg attaatttga tgtacgacct ggtgacctgc cttaccacaa
     1921 cgccgctacg tatgctgagt ctgctcggca gcattattgc gattggaggt tttagcattg
     1981 cggtgctgct ggtgatttta cgcctgacct tcggaccaca atgggcggca gaaggcgtct
     2041 ttatgctatt tgccgtgctg tttactttta ttggcgctca gtttatcggc atgggattac
     2101 tcggtgaata tatcggcagg atctacaccg atgtccgcgc ccgcccccgc tattttgttc
     2161 agcaagttat ccgtccatcc agcaaggaaa atgaataatg aaaaccgtcg tttttgccta
     2221 ccacgatatg ggatgcctcg gtattgaagc tctgctggct gccggttacg aaattagcgc
     2281 catttttacc cataccgata atcccggtga aaaagccttt tatggttcgg tggctcatct
     2341 ggcggcggaa agagacattc cggtttatgc gccggataac gttaatcatc cgctgtgggt
     2401 ggaacgcatt gcccaactgt caccagaggt gattttctct ttttattatc gccatcttat
     2461 ttgcgacgag atttttcagc tcgctcccgc aggtgcattt aatctgcacg gttcgctgtt
     2521 accaaaatat cgtggtcgcg cgccgctgaa ctgggtgctg gtgaacggtg aaacggaaac
     2581 gggcgttaca ttgcaccgaa tggtaaacgt gccgatgctg gggccattgt ggcgcaactg
     2641 cgcgttgcca ttgcgccaga cgatatcgct attacgctgc atcataaatt gtgccatgcc
     2701 gcgcgccagc tactggaaca gacattaccc gccattaaac acggtaatat tctggaaatc
     2761 gcccagcgcg aaaacgaagc cacctgtttt ggtcgcagaa cgccggatga cagtttcctt
     2821 gaatggcata aaccggcatc cgtactgcac aacatggtac gtgccgttgc cgatccgtgg
     2881 ccgggtgcct tcagctatgt tggcaatcag aaattcaccg tctggtcgtc gcgtgttcat
     2941 cctcatgcca gcaaagcaca gccggggagc gtgatttctg ttgcgccact gctgattgcc
     3001 tgtggcgatg gcgcgctgga aatcgtcacc ggacaggcgg gcgacggcat tactatgcag
     3061 ggctcgcaat tagcgcagat gctgggcctg gtgcaaggtt cacgcttgaa tagccagcct
     3121 gcctgcaccg cccgacgccg tacccgggta ctcatcctcg gggtgaatgg ctttattggc
     3181 aaccatctga cagaacgcct gctgcgcgaa gatcattatg aagtttacgg tctggatatt
     3241 ggcagcgatg cgataagccg ttttctgaat catccacatt ttcactttgt cgaaggcgat
     3301 atcagtattc attccgaatg gattgagtat cacgtcaaaa aatgtgatgt cgtcttgccg
     3361 ctggtggcga tagccacgcc gattgaatat acccgcaacc cgctgcgcgt atttgaactc
     3421 gattttgaag agaatctgcg cattatccgc tactgcgtta agtaccgtaa gcgaatcatc
     3481 ttcccgtcaa cttcagaagt ttatgggatg tgtagcgata aatacttcga tgaggaccat
     3541 tctaatttaa tcgtcggccc ggtgaataaa ccacgctgga tttattcggt gtcaaaacaa
     3601 ttacttgatc gggtgatctg ggcctatggc gaaaaagagg gtttacagtt caccctcttc
     3661 ctcccgttta actggatggg accacgactg gataacctta atgcagcgcg aattggcagc
     3721 tcccgcgcta ttacgcaact cattctcaat ctggtagaag gttcaccaat taagctaatt
     3781 gatggcggaa aacaaaaacg ctgctttact gatatccggg acggtatcga ggcgttgtac
     3841 cacattatcg aaaatgcggg aaatcgctgc gacggcgaaa ttatcaacat tggcaatcct
     3901 gagaacgaag cgagcattga ggaactgggc gagatgctgc tggcgagctt cgaaaaacat
     3961 ccgctgcgcc atcatttccc accgtttgcg ggctttcgcg ttgtcgaaag tagctgctac
     4021 tacggcaaag gatatcagga cgtagagcat cgtaaaccga gcatccgcaa tgcccaccgc
     4081 tgcctggact gggagccgaa aattgatatg caggaaacca tcgacgaaac gctggatttc
     4141 ttcctgcgca ccgttgatct tacggataaa ccatcatgac caaagtaggc ttacgcattg
     4201 atgtcgatgc ctttcgtggc acccgtgaag gcgtgccgcg tctgctggaa atcttgagta
     4261 agcataatat tcaggccagc atttttttca gcgtcggccc ggacaatatg ggccgccatc
     4321 tctggcgact ggtgaagcca cagtttttgt ggaagatgct gcgctcaaac gcggcatcgc
     4381 tttatggctg ggatatttta ctggcaggta cggcctggcc aggtaaagag attggtcatg
     4441 ccaatgccga tatcattcgt gaagcggcta aacatcacga agtcggtctg cacgcctggg
     4501 atcaccatgc ctggcaagcc cgtagcggta actgggatcg gcaaacaatg atcgacgata
     4561 ttgcacgcgg tcttcgcact ctggaagaga ttatcggtca accggtaacc tgttctgccg
     4621 ctgcgggctg gcgtgccgac cagaaggtga tcgaagcaaa agaagcgttc catttgcgct
     4681 acaacagcga ttgtcgtggg gccataccgt tccgtccact gcttgaatca ggaaaccctg
     4741 gcactgcgca aattccggtg accttaccca cctgggatga agtgattggt cgggatgtga
     4801 aagcagaaga ttttaacggt tggttactca accgcatcct gcgagataaa ggcacgccgg
     4861 tttataccat tcatgcagaa gttgaatgct gcgcttatca gcataatttt gtggatctcc
     4921 tcaaacgcgc agctcaggaa ggcgtgacat tttgcccttt aagcgaactg ttatcagaga
     4981 cgttgccgct cggacaagtt gttcgcggaa atattgccgg acgtgaaggc tggctgggtt
     5041 gccaacaaat tgcgggtagt cgctgatgaa atcggtacgt taccttatcg gcatcttcgc
     5101 gtttattgcc tgctattacc tgttaccgat cagcacgcgt ctgctctggc aacccgatga
     5161 aacgcgttat gcggaaatca gtcgagaaat gctggcatcc ggcgactgga ttgtgcccca
     5221 tctgttaggg ctacgttatt tcgaaaaacc cattgccgga tactggatta acagcattgg
     5281 gcaatggcta tttggcgcga ataactttgg tgtgcgggca ggcgttatct ttgcgaccct
     5341 gttaactgcc gcgctggtga cctggtttac tctgcgctta tggcgcgata aacgtctggc
     5401 tctactcgcc acagtaattt atctctcatt gtttattgtc tatgccatcg gcacttatgc
     5461 cgtgctcgat ccgtttattg cattctggct ggtggcggga atgtgcagct tctggctggc
     5521 aatgcaggca cagacgtgga aaggcaaaag cgcaggattt ttactgctgg gaatcacctg
     5581 cggcatgggg gtgatgacca aaggttttct cgcccttgcc gtgccggtat taagcgtgct
     5641 gccatgggta gcgacgcaaa aacgctggaa agatctcttt atttacggct ggctggcggt
     5701 tatcagttgc gtactgacgg ttctcccctg gggactggcg atagcgcagc gggagcctga
     5761 cttctggcac tattttttct gggttgagca tattcaacgc tttgcactag atgatgccca
     5821 acatagagct ccgttctggt actacgtgcc ggtcatcatt gccggtagcc tgccgtggct
     5881 gggattactc cccggtgcac tgtacacagg ctggaaaaac cgcaagcatt ccgcaaccgt
     5941 ctatttgttg agctggacga taatgccgct gctgtttttc tccgtcgcta aaggcaaatt
     6001 gcccacctat attctttctt gctttgcacc tctggcaatg ctgttggcgc attacgcgtt
     6061 gctggcagca aaaaataatc ctctggcgct gcggattaat ggctggatta acatcgcttt
     6121 tggcgtcact ggcattattg ccacatttgt gatctccccc tggggaccaa tgaacacgcc
     6181 ggtgtggcaa accttcgaga gctataaagt cttttgtgcc tggtcgattt tttcgctatg
     6241 ggcatttttc ggctggtaca ccttaacaaa cgtcgaaaag acctggtctt tcgccgcgct
     6301 ttgcccgctg gggctggcgt tgctggtagg attttcaatt cctgacagag tcatggaagg
     6361 aaaacatccg caattttttg tcgagatgac acaagaatca cttcagccaa gccgctatat
     6421 tcttactgat agcgtcggtg ttgccgcagg tctggcatgg agcctgcaac gcgatgacat
     6481 catcatgtat cgccagacag gtgagttgaa atacggcctt aattatccgg atgcgaaagg
     6541 gagatttgtc agcggtgatg agttcgcaaa ctggcttaat caacatcgtc aggaggggat
     6601 tattacactc gtgctttcgg ttgaccgcga tgaagatatc aacagtctcg ccattccgcc
     6661 cgcagatgcc atcgttcgtc aggagcgtct ggtgctgatt cagtatcgtc ccaaatgatc
     6721 tggctaacat tagtctttgc cagcttgctt agcgttgccg ggcagttgtg tcagaaacag
     6781 gcaacctgct ttgtggcgat aaacaaacgg cgcaaacata tcgtgctgtg gctgggactg
     6841 gcgctggctt gtcttggtct tgccatgatg ctctggctgc tggtcttgca gaacgtaccg
     6901 gtaggcattg cctacccgat gttaagtctg aattttgtct gggtgacgct ggctgcagta
     6961 aaactgtggc acgaaccggt atcgccgcgt cactggtgtg gggtggcgtt cattattggt
     7021 ggcattgtga tcctcgggag tacggtgtaa tgtgcctgat atggggatta ttcagcgtca
     7081 ttattgcctc agttgcgcaa ttaagcctgg gttttgcggc aagtcatctg ccgccgatga
     7141 cgcacttatg ggattttatc gcggctctgt tagcttttgg cctggatgcc cggatactgt
     7201 tactcgggct gctgggttat ctgctttccg ttttttgttg gtataaaacg ctacataaac
     7261 ttgcgctaag caaagcctat gccttgctca gtatgagtta tgtgctggta tggattgctt
     7321 caatggtact tcccggctgg gaagggactt tttcgctgaa agcactactg ggagtagcct
     7381 gtattatgag cgggttgatg ctgattttcc tgcccacgac aaaacaacgt tactgagttt
     7441 tccctgccgc tgtacactgc cgttcccact catcggggct ataactgctt gccgagagga
     7501 ctttcaccgt atgcagcttt tcacgattaa tacaataaag cgcattctgc aaaggcgaga
     7561 gtaaatcccc cactttcacg gcgaagtcac tcttcacttc ggcgatcatt tttatcgcgc
     7621 cgccagcatc gcagagcata agtacatgtc tgttatcttg tttattgcag cacgattttt
     7681 tgtgccagcc attccattgc attatcctgt ttgctaagag ttttcacatc aatttacaga
     7741 ttaacagata aactctttat caacggcaat ggaatggcag aaatgagaag tgaaacggag
     7801 ggttaattat tgctgacgtt gcacccactc ttttagcgcc tgacgtgaaa ttttaatacc
     7861 gccgtttttc agctccggcg gtaaagtcag ccagcgcacc ggttgttgaa aacgagccag
     7921 cttatctttc acccattcgc caagatcaac ggtttgctgg tcatactcca ccaccgctac
     7981 cggccgatga ccaaactcct tgtcggcaac ggaaacgata aacacctgca aaaccgcagg
     8041 atgtgcagca ataacacgct cgacttcttc cggctgaata ccctcaccgc cactgaagaa
     8101 cagattgtct aaacgtccga caatggtcag cttgccatta tgcatctcac cgcgatcgcg
     8161 cgtagcgtac cagccttcgt cattaaccag tgaaaccagt tgcccgttac gccagtaacc
     8221 ttctgccata ctggcagccc gcagccacac ttcattatta acgattttca cttcccgacc
     8281 cggcagcggc aaaccaacgt ctgccaggcc gtcggcttct ttcgcacata ccgtggaggc
     8341 aaactcggtc agaccatagc cgcaaaagca acgaatcccc tgctcgcgcg cctgttccgt
     8401 caactcgacc gggatagccg cgccgccaag taacaccgct ttcagggaaa cggaactgcg
     8461 gttaaccagc aaacgccaga gttgtgttgg caccagtgaa gcgtgagtac agcctgccag
     8521 catttgctcc aatggctgtt tatcacgcac cgtcatccgc gcaccagcgt ataaccagcg
     8581 ccacataatt ccctgaccgg agacgtgaaa cagcggtaaa gagagcaacc agtcatcgtg
     8641 atcgccaaac ggaatcagcg ataacacacc ttgcgcactg gcaagatggg cctgataagt
     8701 atggacagcg gctttcggca aaccggtaga acccgaggtc aacgtcattg agcacagacg
     8761 cgtcggctgc cacgcagcgg catgtacgcc ttcaaccagc tgaatgtgca gcgacgttaa
     8821 tgccggaaac gtgttttccc catccggcac cagcgcaaat tgcagcgtca gattgggcag
     8881 caattcttca agcaacggtt gcggcagctg agggttcacg ggcaacaccc gcgccccgca
     8941 ttgcagtaac gccagccagg cgagcagcgt ttgcggcgta ttccacgccc gcaacatcac
     9001 gccgctgccc tcaaccaccc cctgcgccgc aaagcccgta gctaactcat cgacgcgagc
     9061 acaaagctcg cgccagttga gttgctcgtc attaagacgt aaggcgatgg cttctccccg
     9121 cacttgccgc cagtgacgcc acggccagtc agagaagatc ataacaactg ctcctgtaca
     9181 tcagcgtcca ctaacggcag tgggctcccc ggccagcgac gaacctgctg cgcctgcatc
     9241 agatccagcg tgtccagtcc cggaatggtg tctggcgtta accaggcagc aatccgcgcc
     9301 agttgcgtta gacctaagct cgactcaatg gaagaactga tcaccgccgt cagccccagc
     9361 gcatgcgccg cctgtacctg ctcgcgtact ttttccagac tgcccgtgag cgtgggtttg
     9421 ataactaccg cgcgcacgcc ctcttcagcc acaaaggcaa aatccggctc gcgcaggctt
     9481 tcatcccagg caatggcaat gccggtttca cgggcaaacg ctcgcgaatc atcgcgggtt
     9541 ttgcacggct cttcgagaaa cgcgatgcgg tggcgataat ccgggttaac atatttggca
     9601 aactgctgac ctttcagcgg tgtccaggcg cgatttgcgt caagacgcaa atgcagatcc
     9661 ggaattgcct ccagcaacag attcaccacc atgccgtcgc gcaccgcttc gtacaatccc
     9721 actttgacct tcgccacttt ctcgcccggc atatcggcaa gtttgaggat cagatcgtcc
     9781 ggatcgccat tacacagcgg tgccgcacgg tagttggctg cttgcggcaa cgtatctgtc
     9841 agttctgcca atgcacagct tacgccaaag gccacggaag gcatctgcgg tagctcgcaa
     9901 tcgcctgcca gccagttatt tacccaggca agcagcacgc tttgcgcctc ttcccagttt
     9961 tcctggctga agcccggcag tggggagatc tccccccacc cttcgcgctc gccctcacgc
    10021 aggcaaacgt acagcccgtc gcgggttttt aaccgcctgt cgcgcagaac cacccccgcg
    10081 tccatgggga tctgccagcg gtatacctgc gcgctacgca ttacggattc cgtttaaatt
    10141 tgctgaaatc aggctgacgt ttctggttga aagcgttgcg accttcctga ccttcttccg
    10201 tcatgtagaa cagcatggtg gcgttgcccg ccagctcctg cagccccgcc tgcccgtcac
    10261 agtcggcgtt cagcgcagct ttcaggcagc gcagcgccat cgggctgttt tgcagcattt
    10321 cgcggcacca acggacggtt tctttttcca gatccgccag cggtaccacg gtgttgacaa
    10381 ggcccatatc cagcgcctgt tttgcgtcgt actgacggca caggaaccag atttctcgcg
    10441 cttttttctg cccgacgata cgagccatgt aggaagcgcc ccagccgccg tcgaaggaac
    10501 cgactttcgg gccagtctga ccgaagatgg cattatctgc tgcgatagtc aggtcgcaca
    10561 tcatgtgcag aacgtgacca ccgccgatgg agtagccagc caccatcgcg acaaccggtt
    10621 tcggacaggt acggatctga cgctggaagt ccagcacgtt caggtgatgt acgccggaat
    10681 catctttata gccgccgtaa tcaccacgca ctttctggtc accaccggag cagaacgctt
    10741 tatcgcctgc gccagtcaga atgatcacgc cgatattgtc gtcataacgc gcatctgcca
    10801 gtgcctggat catctctttt accgtcagag gacggaaggc attgcgcacc tgcggacgat
    10861 taatggtgat ttttgcgata ccgtcggtgg atttttcata acgaatgtcc tcgaaacctt
    10921 cggagcagtc gtgccattca accggtgcgt aaggcattgc ttcatcagga taaatcatat
    10981 tgtgtccttt agtcagaaac gcaagatctg cgccagactt gcgattacgc cagcgggatt
    11041 ttcccgatgc gcgttatgtc cggcgcgagg aatgacatgg cagtcggcag ccagttccgc
    11101 cgccagggcg cggaatttgc tgtcacgttc accacataaa taataaaacg caaatgtgcg
    11161 ggcgctaagg ttagcacgta aatcaggctg gacggcgaga gaagtcgcct ccagcatggc
    11221 ggcaagggtt gcgccattat tgttgctgcg cagcgccacc agctcccggc gttgatcgtc
    11281 attgagcgag gcaaaaacag gctgttgata ccagtcggca aataccgccg ttaacggttc
    11341 tgtgcgaaaa cgctgcgccc attggcgatc ggaacgctga cgttccgcac gttgttcagc
    11401 attttgcagc cccggatgcc cgccttcgac aataacccca caaagccccg ccagcccctg
    11461 gcaagccgcc atcatcgcca cccgtccgcc aagcgagtac cccaccagcc agaagtcaag
    11521 gatgttgtaa ctaaccaagg ttttacgcag taagtcggtg acatcatcaa atccatcgac
    11581 gctaatcgcc gccgaaccac catgacctag gagatcaaca tacaaccgtg agtagtcggc
    11641 aaacgcctcg cccacttctt gccattcgtg gcaatcgccg gaaaaaccgt gcaaaaacac
    11701 cagccagggt aaacctagtt ttccgtgttt tgcctgcgcg tgcaggatca taaatggctt
    11761 acctgcgcca gaagttgctg gagcgtttgc gcaccatcgg tgtcgttaac caccatttca
    11821 atcaccgtgg tggttggcgt gcgccaggcg tcggcaaatg ccgtttcaag ttcctgccag
    11881 ttttgcggat gatgatattt cagctcgaac atcgcggcgg cgtgctcaaa atggacgttt
    11941 tgcggcatca gatagaaacg ctcacgctcg ctttgcggcg ttggcaacag cgagaaaatt
    12001 tgcccgccgt tgttgttcac cacaattaat accagcggcg cagaaacctg acgcaataac
    12061 gccagcgcgt tgagatcgta aagcgcggag agatcgccca caatcgccag cgtcggtttg
    12121 ccgcttgccc gctgaacgcc tgcggcggtc gaaagcagtc cgtcgatacc gctggcccct
    12181 cggttgctgt acaccgggta acctgccgga agttgcgaaa gcgcatcaat cagacgtacc
    12241 accaggctgt taccaacaaa caattgcccc tgttcaggca gatagtcgct gatgcgatgc
    12301 gccagttgcg cttcgccaaa cgcatcacgg cgggcaataa ccgcctgcat tgcctgttcc
    12361 gccaggcgcg ggatttcaac gcaccagggc tggcgttttt ctgccggatg cagctccagc
    12421 cagtcggcaa tattggcaat taagcgacgt ccgcgatggt gtgccggatc aagtcgccct
    12481 tcaatgtcat caacaatcca gtactcttct ggttcacagc ttgcctgcca ttgcaggagc
    12541 cgtttccccg tcaggctact tcccagttgc accacaattt gcgcctgctg cagctcgctg
    12601 gtcgctttgg cattgcctaa ccaaagatcg gcacacggca gcggctgccc ggtttgtgac
    12661 agcacatcgc caatcagcgg ccagccaaga gtttgcgccc acagggcaac ttttttgccc
    12721 tcttccgcac tcatgcgccc ggcaaccacc acgccgcgct tttgtcgcca gaagaaccag
    12781 tcgcgctgtt tttcactttc cagacgaggc gcttcacgca gccacggttt gtcgtcctgc
    12841 caccagtcac ccagacgctg ttgccagcta agcccggtat cgtccatttc gccatacagc
    12901 ggttcagcaa acgggcagtt gatatggact ccccccgcat gaagcgtacc gagagcgtgg
    12961 tcgatggtag aaaccagcca acgtgcgggg atatcctggg tcgggcgcgg caatgaaata
    13021 ctgtgcgtgg ggtgagaggc gaacattccc ggctggcgaa ttgcctgatt cgcgccgcag
    13081 tcaattagct ccggcgggcg atcggcggtt aagagaatca gtttttctcc ggttaacccg
    13141 gcttcaatca gtgccggata gagatttgcc accgccgtgc cggaggtcac aatcaccgcc
    13201 accggctgct tgctgacttt cgccagcccc agcgccagat gccccaaccc acgctcatcg
    13261 aaatgggtgt ggtgaatgaa tgcggaattc tccgccgccg ctaacgttaa cggtgtagaa
    13321 cgcgagcctg gggcgataca gatgtgtctg acgccgtgac gcgttaatgc ttccagaatg
    13381 accgccgccc agcgtcggtt aaatgcgctt actgacatga gtttgtccgg tatcaatatt
    13441 gcggctaagt ataaggagct acaaaaatag attattgata tgaatcggta atgatgcgac
    13501 tcattactat tccatttgta ataaagtacg cagccctgcc gctttgttgt cgatttcctg
    13561 ccactcttgc tcggggtcgg aaccacggac aatgcccgcg ccagcatata atcgcacgac
    13621 attgccgcta atttttgctg agcgcaggga aacgcagaat tcgctttgtt gtaatgagag
    13681 atagcccgca gaaccggcgt accattctcg ggtgaacggt tcgtgacggg cgataaactg
    13741 tcgcgccaga tcgcgcggta agccagcaac tgctgccgtt ggcagcaact gatgtaaaca
    13801 gatcacatca tccgctttgt tgagtgaagt ccagatacag cggcgaagat gctgcacttt
    13861 acgcagacgc agtacctgcg gcggtaaaac atccagcgtc tgggtatcgg cctgtaatcg
    13921 ttggcagata tcttccacca ccagcatgtt ctcgcgctgg tttttatcat ccgccatcag
    13981 ccactctcct aactgctgcg cctgcttatc atcaggatga tttgctactg ttcccgccag
    14041 cgcttcagta cgcagcgctt tgtcacgccg ccgccataac cgttccggtg aagagccaag
    14101 aaaagcattt tcgccatcaa aggccatgta aaaatggtag caattcagat tcagtcgacg
    14161 actggcagcc atcatcgccg ccgcgttgac cggacttgcg aaatgcaggt cagttgcccg
    14221 agcgagcacc actttgtcga gctcaccttc ggcgatggtt ttcgttgcca gttcgattaa
    14281 ttgcgtccag cccgttttgt ccggccagtg ttgttctcgc gtggtggtta aatgtaaccc
    14341 aggcaagggc ttgatactca ccagtgtggc gataaattct tttgcctgaa tcgcatcgtg
    14401 ctgaagggag ctttcgctga atagcgtcag ccgcagcgtg gctttaccgc cacagcgtcg
    14461 ccattccagg cggggtaaaa gtaaattgcc ctgcgacggg tcaaaagcat tcagccccca
    14521 aatgcgtaag tcagcgtgtt ccgggtgctg gcgaagaaaa cgttgtgcct ggtccaacga
    14581 cgtaaaacgg gtaatcgcgc ccaggacggc agcttcttca tcaccattac gttgttgcca
    14641 gtagaattgc gggtacgttt gctgactggc cagccagctc aaggcatcaa aagcgtcttt
    14701 gagagggaaa ggaatatcga taacccgaat gccgggtgtc gccggaatct cttgcgacaa
    14761 atggcgcagt agattttcca gcgccgtagt aagtgattgc acgcggacct ctccctgtta
    14821 aaagcctcac attatacggg gtactacaaa aaaaatgcag taccccggtg tagggaggtt
    14881 ttaacggcgt gccagcaaca gtcctagtac cagcccgacg gccgcgccca caccaattcc
    14941 ttgccagggt ttttcgtgga cgtagtcatc agcacgataa acagcctgct tcgcccgata
    15001 gtaataactg tctgaagcct ggctaacccg ttttttcaca tcatccagcg ctttttctgc
    15061 atgcgctttc agctcaacat atttctgatc ggcgggatcg ccagaggagc ggagcacctc
    15121 ttccagtgtt tcactaagca gcgtcaggtc gtcatcgata cgtgtatcac caaactgatt
    15181 agacattctc gttctccatt tgcgtaaaac ctgtcactaa ctatagacaa gagttgttga
    15241 ttacgcctga attacctcgc gtgccatccc aatgtgtggg ataccatctt cttcatagac
    15301 ctccgtcaca gggataaaac cgaaactctg gtagaagttt tgcagatgcg cctgcgcccc
    15361 gaggtaaaca ggcttatcag gccagtgatg cgtacatgtt tcgagtgttt tactcatcag
    15421 ctgctgcccc actttttcac cacgcaacgc ttcactgaca atcacccgac ctataacgac
    15481 cggctcaaga tcatcatcac ttttcagaat cctcgcatac gccaccagtt catcattttt
    15541 ccaaccgaga atatggcgat tatcccccgt caaatcatca ccatcgatat cctgataagg
    15601 gcaattctgc tcgacaacaa acaccgcgca acgcagttgt aataaggcat aaagctggga
    15661 aaccgataat tcagagtgat gcagatcttg ccattcaatc ataatgctct ccttttagat
    15721 gtatgcctta tactagccgg aatatttttt gaaacgtgat gaactcatgg aattaatttt
    15781 tttaggtact tcagccggtg tgccaacccg cacgcgcaat gtcacggcaa tattgcttaa
    15841 cctgcaacat ccgacccaga gcggactttg gttgtttgac tgcggtgaag gcacccagca
    15901 tcagctactg cataccgcct ttaaccctgg aaaactggac aagattttta tcagtcacct
    15961 tcatggcgat catctttttg gtttacccgg cttgctgtgc agtcgttcta tgtcaggcat
    16021 tatccaaccc ttaacgattt atggtccgca aggtatccgt gaatttgtgg aaaccgcgct
    16081 gcggattagc ggctcatgga ccgattatcc gctggaaatt gtcgaaattg gcgctggcga
    16141 aattctcgat gatggcctgc gcaaagtaac cgcttatccg ctggaacacc cactggaatg
    16201 ttatggctat cgtattgaag aacatgataa accgggtgca ttaaatgccc aggcattaaa
    16261 agctgctggc gtgccgcctg gcccactgtt tcaggaatta aaagcgggca aaacaatcac
    16321 gctggaagat ggaaggcaga ttaacggcgc agattactta gctgctccag tgccaggtaa
    16381 agcgctcgct attttcggcg ataccggccc ctgcgatgcc gcacttgacc tggctaaagg
    16441 tgtcgatgtc atggtgcacg aagcgacgct ggatataacc atggaagcca aagccaatag
    16501 tcgcggccat agctctacac gccaggctgc gacactagcc cgtgaggctg gagtcggcaa
    16561 gctaatcatt acccacgtca gctcgcgcta tgatgacaaa ggttgtcaac acctgttacg
    16621 tgaatgcagg tcaattttcc cggcgactga actggcgaat gatttcaccg tgtttaacgt
    16681 ttaactctgc ttattattaa aaacagggtg aaacttgccc tgttatagca acccgcgcta
    16741 catatatcca cccacacagg catattgagc gatttttgat atttattcat ctccttacgc
    16801 actatattca gagcaataag ttgcctcttt tcatcttgat tcagcacaac ctttgcaact
    16861 aaaaggagcc catgatggtt acagttgtca gcaattattg tcaattatct caaacccaac
    16921 tcagtcagac atttgcagaa aaatttactg tgaccgagga attactgcag tctttaaaaa
    16981 aaacagcgtt atccggagat gaagaaagta ttgagttact gcataatatt gcgttaggtt
    17041 atgatgaatt tggaaaaaaa gctgggtaat gctgccaact tactgattta gtg
//
LOCUS       DAZSIO010000083        16460 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZSIO010000083 DAZSIO010000000
VERSION     DAZSIO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.083"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(193..1386)
                     /gene="tyrB"
                     /locus_tag="ACHX5T_002453"
     CDS             complement(193..1386)
                     /gene="tyrB"
                     /locus_tag="ACHX5T_002453"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR8467615.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1639..2718)
                     /gene="alr"
                     /locus_tag="ACHX5T_002454"
     CDS             complement(1639..2718)
                     /gene="alr"
                     /locus_tag="ACHX5T_002454"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR8467616.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(2771..4186)
                     /gene="dnaB"
                     /locus_tag="ACHX5T_002455"
     CDS             complement(2771..4186)
                     /gene="dnaB"
                     /locus_tag="ACHX5T_002455"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR8467617.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4269..5252
                     /locus_tag="ACHX5T_002456"
     CDS             4269..5252
                     /locus_tag="ACHX5T_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR8467618.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5418..5660)
                     /gene="pspG"
                     /locus_tag="ACHX5T_002457"
     CDS             complement(5418..5660)
                     /gene="pspG"
                     /locus_tag="ACHX5T_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR8467619.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(5794..6831)
                     /gene="dusA"
                     /locus_tag="ACHX5T_002458"
     CDS             complement(5794..6831)
                     /gene="dusA"
                     /locus_tag="ACHX5T_002458"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR8467620.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(7194..7901)
                     /locus_tag="ACHX5T_002459"
     CDS             complement(7194..7901)
                     /locus_tag="ACHX5T_002459"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR8467621.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            complement(7925..8179)
                     /gene="yjbL"
                     /locus_tag="ACHX5T_002460"
     CDS             complement(7925..8179)
                     /gene="yjbL"
                     /locus_tag="ACHX5T_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR8467622.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFENKDATKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            8497..9012
                     /gene="zur"
                     /locus_tag="ACHX5T_002461"
     CDS             8497..9012
                     /gene="zur"
                     /locus_tag="ACHX5T_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR8467623.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(9054..9263)
                     /locus_tag="ACHX5T_002462"
     CDS             complement(9054..9263)
                     /locus_tag="ACHX5T_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR8467624.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(9379..10704)
                     /gene="dinF"
                     /locus_tag="ACHX5T_002463"
     CDS             complement(9379..10704)
                     /gene="dinF"
                     /locus_tag="ACHX5T_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR8467625.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(10777..11385)
                     /gene="lexA"
                     /locus_tag="ACHX5T_002464"
     CDS             complement(10777..11385)
                     /gene="lexA"
                     /locus_tag="ACHX5T_002464"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR8467626.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(11495..11863)
                     /gene="dgkA"
                     /locus_tag="ACHX5T_002465"
     CDS             complement(11495..11863)
                     /gene="dgkA"
                     /locus_tag="ACHX5T_002465"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR8467627.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            12034..14457
                     /gene="plsB"
                     /locus_tag="ACHX5T_002466"
     CDS             12034..14457
                     /gene="plsB"
                     /locus_tag="ACHX5T_002466"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR8467628.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(14612..15484)
                     /gene="ubiA"
                     /locus_tag="ACHX5T_002467"
     CDS             complement(14612..15484)
                     /gene="ubiA"
                     /locus_tag="ACHX5T_002467"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR8467629.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(15497..15994)
                     /gene="ubiC"
                     /locus_tag="ACHX5T_002468"
     CDS             complement(15497..15994)
                     /gene="ubiC"
                     /locus_tag="ACHX5T_002468"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR8467630.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(16217..>16460)
                     /locus_tag="ACHX5T_002469"
     CDS             complement(16217..>16460)
                     /locus_tag="ACHX5T_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8467631.1"
                     /translation="FSSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTCIYKSRHKMLPI
                     NLTQETLFSSKSHGKYALFPIFTASWRATRIKNKGI"
BASE COUNT         4273 a         4361 c         3908 g         3918 t
ORIGIN      
        1 tactccacac gaaaaaatag ggaaaggaat tccagataat tgggataaac ttaacaataa
       61 aagaactgac aacgttttat tatctctcac gtagaacaac ggcatcaaaa aaaatcatct
      121 acaaagaaat agtgaaagcg acgacagttt aatttcactg caggctgggt aactccagcc
      181 tgctttcctg cattacatca ccgcagcaaa cgcctttgcc acacgttgta catttgccgt
      241 atttaacccg gcgacacaca tgcgaccgct ggcgatgaga tagacaccaa attcttcacg
      301 tagtcggtca acctgagcgg cacttaaacc ggtataactg aacatgccgc gctgattaag
      361 cagataatcg aaattgcgtt ctggcatctc tgtgcttaat accttcacca attcctgacg
      421 cattgccaga atgcgagtac gcatctcttc tacttccgcc agccagctgg ctttcaatgc
      481 ctcgtcattc agcactgcag ccaccacctg cgcaccaaaa ttcggcgggc tggagtagtt
      541 gcggcgaact gttgctttca attgccccag tacgcggcca gcggcttcgg catcttcaca
      601 cagaacagaa agtccgccga cgcgctcgcc gtaaagggag aaaattttcg agaacgaatt
      661 gctcaccaga gcgggtaatc cagcactggc aatggcgcga atagcgtagg catcctcttc
      721 cataccggca ctaaatcctt gataggcaat atcgaggaat gggataagct cgcgggcttt
      781 gagaatttca atcaccgcat cccactgatc attagtgaga tcggcacccg ttgggttgtg
      841 gcaacatgga tgcagcaaca caatactgcg ggcaggtaat gttttcagcg tcgccaacag
      901 gtcattaaag cgcacgctgt tagtcacttc gtcataccag gggtaagtac tcacttcgaa
      961 tccagccccg gcgaatattg ctacgtggtt ttcccaagta ggatcgctga cccagacgcc
     1021 tgattccggg aagtagcgtt tcaggaaatc cgcgcccact ttcaatgccc ctgagccgcc
     1081 aagggtttga atggttgcta cgcgctgttg ttgcagtacc ggatggtccg caccaaacag
     1141 cagcggcgca atggcatggc gatagctgtt aagcccttcc atcggtaaat aaagcgaagc
     1201 gccatggggc tgcgcattca ggcgcgcttc cgcctccgcc acggctttca attgtggaat
     1261 aattccgtct tcgttgtagt acagaccgat acttaaattc actttgtcgc tgcgagggtc
     1321 ttctttaaaa cgctccataa gcgtaagaat cgggtcgcca gcgtaggcgt caactttttg
     1381 aaacacgcga tggttctcca ggtttacggg caggtggtta aaacacaata aaccggaaga
     1441 aggcgaagat cgagtggatg ttcaggagcg aacggcaatt agcaacagag tgagactaat
     1501 gataaacgta catccgccag aggcacgacc tttataagag catgaaagaa catcaactta
     1561 ttgaattttt aggattttat tggccggata aggcattcac gccgcatccg gcacagacaa
     1621 tcaaatatta cagaacgatt aatccacgta tttcatcgcg acccttgaag tcaggcgcgt
     1681 aataagttcg taagcgctta ctttcgtcat ttcagcgata cgttctacgg gcaaaccttc
     1741 gccccataaa atgaccggat ccccggcttt gtcctgcgcc tgtggaccta agtctacgca
     1801 gatcatatcc atcgccacgc gcccgacaat cggtacttcg cgaccgttca ccagcactgg
     1861 cgtaccggac ggcgcggcgc gcggataacc atcgccatag cccatcgcga ctacgccaag
     1921 acgggtatca cgttcgctta cccaggttcc accataacca acaggctctc cggctttatg
     1981 ctcacgcacg gcaatcaggc tggaggttag tgacatcact ggctgacagc caaaatcggc
     2041 accggtggag cgatcttcca gcggcgagac gccataaaga atgatgcccg ggcgcaccca
     2101 gtcaaaatgc gactgtggcc acagcagaat gccacccgac gcggcaatgg aacgttgacc
     2161 aggtttgcct tcgcaaaagg tattaaagat agcgagttgt ttctcggttg cgccacattt
     2221 tggttcatcc gcgcgcgcaa aatggctgac gatattcacc ggctgacgaa cgtttttgca
     2281 ctgggtcagg cgatgataaa acgcctcagc ctgttccggc cttacgccca gacggtgcat
     2341 accggtatcg agtttcatcc agacggtaac cggctcgtcc aagctagcct cttccagcgc
     2401 agccagctgt tcttcgttat gcacggcggt atgaaaatgt tgcgcagaaa tcgtcggcag
     2461 atctctggca tcaaaaaagc cttcgagtaa cagtacaggt ttggtgattc cccccgcacg
     2521 cagtcgcaga gcttcttcga gacgggctac gccaaaggcg tcagcatcgg ggagcgttcg
     2581 cgcggtctca agaagaccgt gaccataagc gttcgctttc accaccgcaa ccattttact
     2641 ggcaggtgcc agttcacgaa gacgttgcag gttgtgtcgc agagcgcggc ggttaatcac
     2701 aacagttgcc gcttgcattt gtgttccttg ataattgttt gctttaatta cctaattcat
     2761 aaaataatta ttattcgtcg tcgtactgcg gccccgcata gttgtcgaag cgcgaccatt
     2821 gaccgttaaa ggtcaggcgt accgtcccga ttgggccgtt acgttgttta ccgataataa
     2881 tttccgcgat gccttttaaa tcactgtttt cgtgatacac ctcatcacga tagataaaca
     2941 tgatcaagtc cgcatcctgc tcgatagagc cagattcacg caggtcggag ttgaccggac
     3001 gtttgtcggc acgttgttcc agagaacggt tcaactggga cagcgccacc accggcacgt
     3061 tcagttcttt cgccagtgct ttcagcgagc gggagatttc tgcaatttcc agcgtacggt
     3121 tatcggaaag cgccggtacg cgcatcagtt gcaggtagtc gatcatgata agcccgatgc
     3181 cgccgtgttc acgggcaata cggcgtgcgc gggaacgcac ttccgttggc gtcaggccgg
     3241 aagagtcatc gatatagata ttgcgttttt cgagcaaaat acccatggtg ccggaaatgc
     3301 gcgcccagtc ttcatcatcg agctgcccgg tacggatttt agtctggtca acgcgcgaca
     3361 gcgacgccag agaacgcatc atgatctgtt ctgatggcat ctccagcgag aagataagta
     3421 ctggtttatc ctgcaacatt gccgcgtttt cgacgaggtt catcgcaaat gttgttttac
     3481 ccatcgacgg acgcgcggcg acgatgatca aatccgacgg ctgcaagcca gcggtttttt
     3541 tgttgagatc gtcataaccg gtgtttaccc cggtaacgcc atcgtgtggc tgctgaaaca
     3601 actgctcaat acgcgccacg gttgcgtcga gcacatcggc gatgttcttc ggcccttcgt
     3661 ctttgtttgc acgactttcg gcaattttaa agacgcggga ttcagcaagg tccagcagat
     3721 cttcgctggt acgcccctgc ggatcaaaac cggcttcagc aatctcattc gcaaccgaga
     3781 tcatctcacg gacaacggca cgttcacgca cgatgtccgc ataagcactg atgttcgccg
     3841 cacttggcgt attttttgac agctctgcca gataagcaaa accaccgacg ctatcgagtt
     3901 gcccctggcg ttccaacgat tccgcaaggg taatcagatc gataggacta ccgctttcct
     3961 gcaaacgcgc catttcagta aagatatgac ggtgtgggcg ggtgtaaaaa tcgtctgcta
     4021 ccacacgctc ggctacatca tcccagcgtt cgttatccag cattaaaccg cccaacaccg
     4081 actgctccgc ttcgatcgag tgcggaggca ctttcagccc ggcaacttgt ggatcgcgtt
     4141 cgcggggttc agcctgctgt ttgttgaagg gtttatttcc tgccatagtg aatggagtta
     4201 ccgaaataaa gaatgggtcg aaactttacc atatgaagca gaccctgacg atacgttctg
     4261 gaggacacat ggcaacacga attgaatttc acaagcacgg tggcccggaa gtacttcaag
     4321 ccgtagagtt cactcctgcc gatccggcgg agaatgaaat ccaggtcgaa aataaagcca
     4381 tcggcatcaa ttttatcgac acgtatatcc gcagcggcct ttacccgccg ccatcgctac
     4441 ccagcggatt aggtaccgaa gcggcaggca tcgtgagtaa agtcggcagt ggtgtaaagc
     4501 atattaaggc tggcgatcat gtagtctatg cgcagtcggc gttaggcgct tacagctctg
     4561 tgcataacat taatgcggat aaagcggcga ttctgcctgc ggcaatttct tttgagcaag
     4621 ctgcggcatc cttcctgaaa ggcttaacgg tttattatct gctgcgcaaa acctatgaaa
     4681 ttaaacccga tgaacagttc ctgttccacg cagcggctgg cggcattggc ttaattgcct
     4741 gccagtgggc aaaagccctg ggcgcgaaac tcatcggcac cgtaggaacc gcgcaaaaag
     4801 cgcagagcgc gctaaaagcc ggcgcgtggc aggttattaa ctatcgtgaa gagaatctgg
     4861 tcgagcgatt gaaagagatc accggcggta agaaagtgcg cgtagtgtac gattccgtgg
     4921 gcagagacac ctgggaacgg tcgctggatt gcctgcaacg ccgcggctta atggtcagtt
     4981 ttggcaactc atcaggtgcg gttaccggtg tgaacttagg cattctcaat caaaaaggct
     5041 cgttgtatgt gacacgccct tccctgcaag gctatatcac cacgcgggag gaattaaccg
     5101 aggccagtaa tgaactgttc tctttgattg ccagcggtgt gattaaggtc gatgtcgccg
     5161 agcagcagaa atatccgctg aaggatgcgc agcgtgcgca tgagattctg gaaagccggg
     5221 cgacgcaagg ttccagcctg ctgattccat aaaagaaata gggcttccac ctgggaagcc
     5281 ctttcttttt atagttcggc tgtatgtagg gtacagcacg atgaatctgt tagaggcgca
     5341 atagtgacag atttgattat caattcctat tttgttctaa ggataaaacc ttaggttgtg
     5401 atcatccgca caatccctta gtaacgccag cggtcataac gctgatattt cggcattttt
     5461 ggtgctttaa tcgccttaat aacccacacc accgcaatcg ccagtagtaa ccacggcagc
     5521 aacttaatca tcaatgccag cataccgccg aggaacataa tggccgtcgc cacaaccagc
     5581 gcggcgataa tgcccagcaa cgaaacgccg gtgaccatca gcatgacaaa aaagccaatc
     5641 acaaaaagta gttccagcat gatgctctcc caaatatgaa atctcttgct ggcattacaa
     5701 gaatcatgcc aaaaataatc tattgattta acagcaaaac gccccgcgac ggtgcgcagg
     5761 gcgtggtgaa tttgactact ttttggtgaa aagttaacgc ttatccgcca ccagtttgag
     5821 cgcgtgttcc agcacgttaa tgtcagcacc cgctttatgg gcattttcac ttaaataacg
     5881 ccgccactgc cgcgcgccag gaataccctg gaacaaaccc agcatatgcc ggataatatg
     5941 gccgagatac gtcccctggc tgagttcatg ctcaatgtac ggatacatgg cacgcactac
     6001 tgccaccgga tcggcatcgg tatccgagga accaaagatc tcccggtcta ccgccgccag
     6061 aatacccgga ttctgatacg cctcgcgccc gaccatcacg ccatccatat gttgcaaatg
     6121 cgctttagct tcttccagcg acttgatacc accgttaatc gacattgtca gatgcgggaa
     6181 gtcacgcttc agttgataca cacgcggata atcgagcggc gggatttcac ggttttcttt
     6241 cgggcttaac cccgaaagcc aggctttacg tgcgtggatg atgaacatct cacactcgcc
     6301 tttgccggaa acggtattga tgaaatcgca gagaaattca tagctgtcct ggtcgtcgat
     6361 gccaatacgc gttttcaccg tcaccggaat cgacaccaca tcgcgcatcg ctttcacgca
     6421 gtcggcaacc agctgcgcat tacccatcag acacgcacca aacatgccgt tctgcacccg
     6481 gtcagacggg cagccgacat tcaggttgat ctcatcatat ccgcgcgctt ctgccagctt
     6541 tgcacactgc gccagcgccg ccggatcgct accgcccagt tgcaacgcta ccggatgttc
     6601 ttcttcactg tacgccagat aatcaccttt accgtgaatt atcgcccctg tggtcaccat
     6661 ttcggtataa agcaacgtat tgcgggaaag cagacgcaag aaatagcggc aatgtctgtc
     6721 cgtccagtcg agcattggtg caacactaaa ccgaccactc cagtgaacgt cagttttttc
     6781 aggcattgcg ctggtttggt tgattttttg catttcagaa ttaccgtgca ttttcaaatg
     6841 tagagattat tttatcgata tatcatgggg ttatgttatt cagcttcact gttcaggagg
     6901 ctcaatagcg gggtactata ccataacaac aggaagcgcc tgtctcattg caaaagaaaa
     6961 ttgagatcct ctcaaggcat gaagctctca cgaagtgatg gaaataatct tattagccgt
     7021 taactttgtt aaggccaatg ataaacaatc caggttcgac gataaataaa aaatcacaca
     7081 ttaaactctg gtgatatatc tccctgctaa tagcattgat agagaaaaaa gaacccaata
     7141 aatattgggt ccttttatat aatgccttcc atactatcga agaacttcac atattatttc
     7201 tccgatttaa ccccgaacaa gtcataaatt aatttagaag tgtctgtaag tattttaatc
     7261 tcttcctttg aggttgggtc aaacgacttc gcgaagctaa ttaattgtgg tgcagcatct
     7321 cgcattttgc ttaaaatatc aggatcgagc gttccttcat tcaccagatg tgacatctta
     7381 tccagatagc catcaaaatt catttctccc ccatctgcca gacgcttcat ctctcccagg
     7441 tacttcttca tatcacgttg atataatttt tcaaactgag ctttcaggta gctctcatta
     7501 ttttcattaa tatacactgt ttctgataaa tctgcgtagg cactagaata tgaaagtgaa
     7561 attaacagcg aagcaaataa ttttaagctg ttgtcatcat gttcctggca ggcattaaca
     7621 aacgttaaaa aaccagaacc tattgcttga aaatgtatat tcgctaaagg ttcattatcc
     7681 ttagattcct cataaaatag ctgaaccgtg gagggaaggc cattcttagt ttctacatca
     7741 aaagtacatt gttcaatggg cattgcatct ttattatccc caggattaca tatactatta
     7801 atcgcgaaat gaaaaatagc ccggtcaaca actgatgcaa taaagtcacg atcgtttaaa
     7861 tcctcatcag tgcaatcgtc agtatactta ttgacccaca ttttgagatc atttatgtgc
     7921 tcaattaaaa acacattttt ttacgtgcat gccataaatt atgcgtgatt cgttgaataa
     7981 acaaatctat ttttgcaata attatatccc gatgacgcag cgctgcataa tcaatagatt
     8041 taccatatat attatttaat ttacctttga ttgaacctct attatccaat ttatctttgg
     8101 ttgcatcttt attttcgaat tgatttgaat tgagtgtttt attaaagtat ccagttgcac
     8161 cgggtattat cttcaacatg attcatctac cttttttgtc gtaccgtgtc ctccttataa
     8221 ttcttttgcc atctggtcac tatcaaaaat cgctcataat ttctgaacta caggtatgcg
     8281 tttaaaccca gttaaataaa tagcgagtta acttgaagca atttatttaa aaatgaatta
     8341 ttaattatta tcgtgttgat aatggtacga taaggcaaca caacacctta cctacaaaac
     8401 agaaaatcaa cattacggca acaataaggg ttctcggtgt ttttcctgga acatggtaaa
     8461 gtaaggacat tcttaacccc cactttgagg tgcccgatgg aaaagaccac aacgcaggag
     8521 ttattagcgc aggctgaaaa aatctgcgcg cagcgtaatg tgcgcctgac cccacagcgc
     8581 ctggaagtgt tgcgcctgat gagtctgcag gatggcgcta tcagcgctta tgatctgctt
     8641 gatttactgc gcgaagctga accgcaagcc aagccgccaa cggtttatcg cgcgctggat
     8701 tttctgcttg agcaaggttt tgtgcataag gtggaatcca ccaacagtta tgtgctctgt
     8761 catctgttcg accagcccac gcatacgtca gccatgttta tttgcgatcg ctgcggcgcg
     8821 gtgaaagaag agtgtgcaga aggcgtggaa gacattatgc atacgctggc ggcaaaaatg
     8881 gggtttgccc tgcggcataa tgtgattgaa gcacatgggc tctgtgcggc atgcgtggaa
     8941 gtggaagcgt gtcgtcatcc tgaacagtgc cagcatgatc actctgtgca ggtgaaaaag
     9001 aaaccgcgtt aagagggcgt acatccttgt acacgtcggg caggagggat taattaccag
     9061 cgatattcat tgcgggtttc ccaatccacg acctcttttt ctgcctgatc tttctgataa
     9121 ccataacgtt cctggatttt accgaccagt tgatcacgtt taccttcaat gatcgtcata
     9181 tcatcatcgg tcagtttgcc ccattgctct ttcactttac ctttaaactg tttccagtta
     9241 ccgccggctt catctttatt cataatcaag acctcatcgt taggttgtga atgagagtac
     9301 gttcactttt cttctgaacg tgagattaag tgtagtcagc aatttggctt aggataattt
     9361 attcagaatt tttaaccgtc acgttgcggc aaaccaggta ccgttgcgcc agtgacgccg
     9421 ccagatagcc gccagagaaa gcccgcgcaa cgccagaaag acggttaatg ccagccacaa
     9481 accatgatta cccagccacg gcagcgtaag gagcgtcagc gcaaaacctg cggcggccac
     9541 cgccatactg ttacgcattt cggcggcacg cgttgcgcct ataaacatgc cgtccagcag
     9601 ataacaccag acgccaacca acggcaaaat cacctgccag ataagatagc ggtcagccag
     9661 ctgctgaatc tgggttaacg acgtcagcaa cgcaatgatg tgttcccctg ccagcaaata
     9721 aaccaccgaa aatagtaacg ctacaatccc cgactggcgg cacgctgccc gccatacatc
     9781 cagcaactgg ctaccgtcgc gcgcaccgta tgcctgaccg gagtgcgctt caaccgcgta
     9841 ggcaaaacca tcaagcgcat aggcggtaaa ggtgagtagc gtcatcagaa ccgcgttaac
     9901 agcgataatg tcactcccca gtcgcgcacc aagtacggtg atcgcgccga aacagagttg
     9961 caacaacagc gaacgcagca tgatatcgcg gttaagcgcc agcaagcgac ggaagtttcc
    10021 tcgccaggca gttttcagca tttcgccgga aattccgcgt agtttgagga ttttacgcac
    10081 cattagcaga ccaatcagca atgttgcata ttccgcaata accgtcgcca gcgccgcgcc
    10141 ctgcacgttc atatgcagcc ccatcaccag ccagacatcc agcacaatgt tgaggatatt
    10201 gccgaccact aacaaaatta ctggcgcacg ggcatattgc acgccgagta accaaccgag
    10261 taataccaga ttcgccagcg acgccggagc gcttaaccag cggatttcaa gaaagcgccg
    10321 cgcctgttcc aggaccgctt cactaccgcc aacaatatgc agcgccagat cgataatcgg
    10381 cgtacgcaac agcgcaatta acgccccggc ccccaacgcc aacagcaacg gttgcaccag
    10441 cgcacgggct aatgcctgag gatttttggc accataagcc tgcgcagtca gcccggtggt
    10501 gctcatgcgt aaaaacagca acagcataaa gagaaagctg gtcgccgttg caccaaccgc
    10561 cacgccgccc aaataaaccg gactatcaag atgaccaatt accgccgtat cgaccagtcc
    10621 cagcaacgga acggtgatat tggagaaaat catgggtaag gcgagatgcc agagtgcttt
    10681 atcagatgaa gtgaggaatg ccatgcagac aagcctgatg aagagagatg aaaaacaaac
    10741 cgcgacgcca agcggcatcg cggtctcaga gatatgttac agccagtcgc cgttgcgaat
    10801 aaccccaacc gccagccctt caatggtgaa gctctgctga cgaaggtcaa cgacaattgg
    10861 tttaaactcg ctattttctg gcaacagttc gactttattg ccctgttttt tcaggcgctt
    10921 aacggtaact tcgtcatcaa tacgtgcgac aacgacctga ccgttacgta catcctgagt
    10981 tttatgcact gccagcaagt cgccatccat aatgccgata tctttcatcg acatcccgct
    11041 gacgcgcagc aggaaatcag cattcggctt gaacaaggaa ggatcgacct gataatgacc
    11101 ttcaatatgc tgttgcgcca gaagcggttc accggcagcc acacgaccta ccagcggcaa
    11161 cccttcttcc tcttcctgca acagacgaat cccgcgtgat gcgccggaaa caatttcaat
    11221 aacgcctttg cgtgccagcg ccttcagatg ttcttcagcc gcgtttgggg aacggaaccc
    11281 caaacgctgc gcgatttccg cacgcgtcgg cggcatacct gtctggctga tgtgatcacg
    11341 gatgagatca aacacctctt gttgcctggc cgttaacgct ttcattccgc cccctgggtg
    11401 tatatacagt tatactgtga gtatatacag caaaaggcga ttttggaacc ataaactgca
    11461 caataaacca gagatttatc gaaatctgga agggttatcc aaaatgcgac cataacagga
    11521 tgcaccaggt aatcacggcg acgataatgg caatcagcac cgcggcggat cccatatctt
    11581 ttgcgcgtcc ggaaagctca tggtattcag agccaattcg gtcaaccact gcttcgatgg
    11641 cgctattgag gatttccaca atcatcacca gcatcacgga gctgataagc agcacgcggg
    11701 taattgcgtc cacatccagc cagcaggcga tgaccaccgc caacaatacc gctacgcctt
    11761 cctgacggaa tgccgcttcg ttgatccatg cagcgcgtaa acctttccag gaatagccag
    11821 cagctttgat aattcgggtg aatccagttg tattattggc cattgaaaga acctttttac
    11881 attatgagcg tcaatatcag tgtaccgtta aatccgatga ttcgggtttg cgtaacgccg
    11941 cgcgaaacat gagcggatac cacagaattt cccatgactt tctgctatcc ttgccgcgca
    12001 tttgcattat taaccagagg ctttacatcg tttatgtccg gctggccacg aatttactac
    12061 aaattactga atttaccatt aagcatcctg gtaaaaagca agtctattcc ggcagatcct
    12121 gccccggaac tggggctgga tacctctcgt ccaattatgt acgttttacc gtacaactcg
    12181 aaagcagatt tgctgacgtt gcgcgcccag tgtctggcac atgatttgcc tgacccgtta
    12241 gagccgctgg aaatcgacgg cacgctactg ccgcgctatg tgttcattca cggcgggccg
    12301 cgtgtattca cctattacac gccgaaagaa gagtctatta agcttttcca cgactatctc
    12361 gatttgcacc gtagcaaccc aaatctggat gtgcagatgg tgccagtatc ggtgatgttt
    12421 ggtcgcgcgc cggggcgtga aaaaggcgaa gtgaacccgc cgctgcgtat gcttaacggc
    12481 gtacagaaat ttttcgctgt actgtggctc ggtcgcgaca gttttgtgcg tttctcgccg
    12541 tcagtgtcgc tgcgccgtat ggcggatgaa cacggcacgg ataaaactat cgctcagaaa
    12601 ctggcgcgcg tggcgcgtat gcactttgcc cgtcaacgtc tggctgccgt aggcccacgt
    12661 ctgcctgctc gtcaggatct gtttaataag ctgctcgcct cccgcgccat tgccaaagcg
    12721 gtagaagatg aagcgcgcag caaaaaaatc tcccatgaaa aagcgcagca gaacgcgatt
    12781 gcgctgatgg aagagattgc ggcgaatttc tcttacgaga tgatccgcct gactgaccgc
    12841 attctgggct tcacctggaa ccgattttat cagggcatca acgtccataa cgccgagcgc
    12901 gttcgccagc tggcccacga cggtcatgag ctggtgtatg tgccttgcca ccgcagtcac
    12961 atggactacc tgctactttc ttacgtgctg taccaccagg gactggtgcc gccgcatatc
    13021 gccgccggga tcaacctgaa cttctggccg gccgggccga ttttccgccg tctgggggcg
    13081 ttctttattc gccgtacttt taaaggcaat aaactttatt ccaccgtttt ccgtgagtat
    13141 ctcggcgaac tgttcagccg tggttattcc gtcgagtact tcgtggaagg cggtcgttcc
    13201 cgcacggggc gtttgctgga tccgaaaacc ggtacgctgt cgatgaccat tcaggcgatg
    13261 ctgcgtggcg gcacgcgtcc gattacgctg attccgatct atatcggtta tgagcacgtc
    13321 atggaagtgg gtacttacgc caaagaactg cgcggcgcga cgaaagagaa agagagcctg
    13381 ccgcagatgc tgcgcggttt aagcaagctg cgtaatctcg gtcagggtta cgtcaacttc
    13441 ggtgaaccaa tgccgttgat gacctacctt aaccagcacg taccagactg gcgtgaatct
    13501 atcgatccca tcgaagcggt gcgtccggcc tggttaacgc cgacggtcaa taatattgct
    13561 gccgatctga tggtacgcat taacaacgca ggcgcggcaa acgccatgaa cctgtgctgt
    13621 actgcgctac tggcatcacg tcagcgctca ctcacccgcg agcagttaac cgagcaactc
    13681 aactgctacc tggatctgat gcgcaacgta ccttactcca cggactctac cgttccttca
    13741 gccagcgcca gcgagcttat cgatcacgcg ctgcaaatga acaagtttga agtcgagaaa
    13801 gacactatcg gcgacatcat cattctgcca cgcgagcaag cggtgctgat gacctactat
    13861 cgcaacaaca ttgcgcatat gttggtgctg ccttcgctga tggcggcaat tgtcacccag
    13921 catcgccaca tctcccgcga cgtattgatg gagcacgtca atgtgcttta cccaatgctg
    13981 aaagcggagc tgttcctgcg ctgggatcgc gacgagttgc cggacgttat tgatgcgctg
    14041 gcaaatgaga tgcaacgtca ggggctgatt accctgcaag atgatgagtt gcatatcaac
    14101 ccggcgcatt ctcgcaccct acagctgctg gccgcaggcg cgcgcgaaac gctgcaacgt
    14161 tatgccatca ccttctggtt gttgagtgcc aacccgtcga tcaaccgcgg tacgctggag
    14221 aaagagagcc gcaccgttgc gcaacgtctc tccgtgctgc acggcatcaa cgcgccggag
    14281 ttcttcgaca aggcggtgtt cagttctctg gtgctgacgc tgcgtgatga agggtatatc
    14341 agcgatagcg gcgatgccga accagcagaa acgatgaagg tttatcagtt gctggcggag
    14401 ttgattacat cagacgtgcg tttgacgatt gagagtgcga cgcagggcga agggtaatca
    14461 gagagaattg ccggatgcgg cgaaaacgcc ttatccggcc taccatgacc tgcaaattca
    14521 ataaattgcg attcaccggg taggcctgag aagcgcaacg catcaggcaa cccagaagaa
    14581 agccggatga tcatccggct tttttacatc atcagaaatg ccagtaactc attgccagtc
    14641 ctaaaaacag taccagacca acatagttat tattcataaa tgctttaaag caggcttcac
    14701 gctcgcggtt ggcaatcagt ttttgttgat aaacaaacag cgcgccagcc accacaattg
    14761 accagtaata tccccagcct aagccattta actcaccgat gatcgccatc agtgccagta
    14821 cgccaatctg caaaatacca ataatcaatt tatcgtattg gccgaacagg attgccgtgg
    14881 atttaatgcc aatcttcaca tcatcatcgc ggtcaaccat cgcatactgc gtgtcgtaag
    14941 ccaccgccca gagaatattg gcgaggaaca ttaaccagca actcaatggc accgactcac
    15001 tcacagcggc aaaagccatt ggaatcgacc agccaaacgc cgcgcccagc accacttgcg
    15061 ggagatgggt ataccgcttc ataaacgggt acacccacgc caatgccaga gcggcaatcg
    15121 acaacagaat ggtcatcgta ttcagcgtca gaaccagtaa aaacgaaatc agtaccagca
    15181 ctacaaacag cgcgcgcgcc tctttctctg ttaccgcgcc gctgggaagt ggtcggttcg
    15241 ccgtgcgctt aacatgacca tcaaacttgc gatcagcata atcattcacc acgcaaccag
    15301 cagcgcgcat cagccagaca cccgcgacaa acaccgccat gatccagagc tgaggaacgc
    15361 ccggtgtcgc cacccacaac gcccataatg ttggccagag cagcagtaac gcgccaattg
    15421 gcttatccgt acgcattaag cgatgaaacg ccagcagctt attctgcgtc agactccact
    15481 ccatattttt ttcctcttag tacaacggtg acgccggtaa aaacagttct gttagcaaca
    15541 gcggtttacc gcttaatcgc aggcgggaac gtcgccccca cagtccggca tcacggccta
    15601 tctcgataaa atcccgggtt agtgtcgatg atgtgaacag atagcgtcct aacggcgttt
    15661 tacccaattt ttgtaacgct agttccggcc cgcttaatgt tgatacagga acgacggtac
    15721 gaccggcaag ccacggttca ccatcggcac ataacaaaat ttcacgtaac cagtaacgag
    15781 actctttcgg cagcagcggc agttcttcgg ggatttcatt ctgctcgaca aacccctcgc
    15841 ggatcatcgt cacactcacc gtttttccct gctgttcaaa acgttttgtc atggaatcct
    15901 ccagcaacag ccagtcgagc agttgcggat ccagggcagg gatctcttta aaatagcgca
    15961 gcgcacgcag ttgcgttaac gcggggtgtg acatgccgaa ctctccgtta cataacgtaa
    16021 aggcattgta tcgcagaaaa gtgtttacgg gagatgtaaa gcaaattaag tgatctaatg
    16081 cgcaacaaaa acgcaacaat cgcatgaagt ggtaaaacgc tgttgaacct tacgctttgc
    16141 cgatgcccgt gctggcgagg gcaaccatag gggcatcacc gcccatgatg ctaggaacgg
    16201 tgatgttttc ctttacttaa atacctttat tctttatacg agtagctcgc caactcgcag
    16261 taaaaattgg gaaaagcgca tatttaccat gagatttgga gctgaaaagt gtttcctgag
    16321 taagattaat aggcaacatc ttatgtctgg atttatagat acacgtggaa ttccaattgg
    16381 tatcttttgt aggattcaac atatcatgta agcgctggga gcttaccacc ataacaggcc
    16441 catcatcgac agaggagaac
//
LOCUS       DAZSIO010000084        16345 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZSIO010000084 DAZSIO010000000
VERSION     DAZSIO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.084"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..1025)
                     /locus_tag="ACHX5T_002470"
     CDS             complement(<1..1025)
                     /locus_tag="ACHX5T_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8467632.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1127..2107)
                     /locus_tag="ACHX5T_002471"
     CDS             complement(1127..2107)
                     /locus_tag="ACHX5T_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFR8467633.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2115..2825)
                     /locus_tag="ACHX5T_002472"
     CDS             complement(2115..2825)
                     /locus_tag="ACHX5T_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR8467634.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3042..4865)
                     /gene="typA"
                     /locus_tag="ACHX5T_002473"
     CDS             complement(3042..4865)
                     /gene="typA"
                     /locus_tag="ACHX5T_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR8467635.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5238..6647
                     /gene="glnA"
                     /locus_tag="ACHX5T_002474"
     CDS             5238..6647
                     /gene="glnA"
                     /locus_tag="ACHX5T_002474"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR8467636.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6820..7869
                     /gene="glnL"
                     /locus_tag="ACHX5T_002475"
     CDS             6820..7869
                     /gene="glnL"
                     /locus_tag="ACHX5T_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR8467637.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7881..9290
                     /gene="glnG"
                     /locus_tag="ACHX5T_002476"
     CDS             7881..9290
                     /gene="glnG"
                     /locus_tag="ACHX5T_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR8467638.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9402..9512
                     /locus_tag="ACHX5T_002477"
     CDS             9402..9512
                     /locus_tag="ACHX5T_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR8467639.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="ACHX5T_002478"
     CDS             complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="ACHX5T_002478"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR8467640.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="ACHX5T_002479"
     CDS             complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="ACHX5T_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR8467641.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(11931..12097)
                     /locus_tag="ACHX5T_002480"
                     /pseudo
     CDS             complement(11931..12097)
                     /locus_tag="ACHX5T_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12394..13026
                     /gene="yihA"
                     /locus_tag="ACHX5T_002481"
     CDS             12394..13026
                     /gene="yihA"
                     /locus_tag="ACHX5T_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR8467642.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13408..16194)
                     /gene="polA"
                     /locus_tag="ACHX5T_002482"
     CDS             complement(13408..16194)
                     /gene="polA"
                     /locus_tag="ACHX5T_002482"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR8467643.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
BASE COUNT         3953 a         4220 c         4013 g         4159 t
ORIGIN      
        1 gcttcgccga taggtgcaaa aaatacggct cgctgtgtaa agactatcgc gccaaagccc
       61 agcgtacatg ccatccctaa atagaccggc atactttcgt gcggcagcat aatcaacagt
      121 acgagcgcag cggtactgat gataaaggtg tagcatagat atttactcgg cgatttcagg
      181 atcttatctg aaatcatgcc accaatcggt ccaccaatca ttttcagaca gtattggtta
      241 atgatgccgt aagcccccac cagcgcaacg ggcaatagat agatgttttt caggaatgga
      301 atgaagaatg tcaggccaca gtaaaccgcg taaacgaaga agacgttgaa agcgataagc
      361 cagatggttt tgtctctcag cactgaggtc atactggtgt tttgcgatgc tccgtcttct
      421 tttttcacct caacggacgg tgcctcttct ttgtcattca ggacaaagaa aataataatg
      481 cctacggcaa tcacaataag ggaatagaac cagatgcctg ctttaaaacc taataagcca
      541 ctgccaaacc aggtaaatac tgccaacgca gaaaatgcca ccacggtatc gacaatgcca
      601 cgccctgttt cgaagaagcc aaacaaccga ccttgttgtt cgctattgcc caatcgactt
      661 accgacttga gcaagaccgg ccagttcatc atgtcgcaag taacgccaaa tagcgcccag
      721 acaaagagga tgccccaata ccccggcatt gtcgtcaaat aaacacccag taatcctgtc
      781 gcaatgagtg agaatgacat ggtgtatctg cgcggtagtt tatcggcaaa ataaatagac
      841 agaaaaaagc ccactgtggt gacaaatgag tttaccgaca tagcattacc aatttgacca
      901 ttggtcaaat ggaaatattc ctgcatcggg atataaaacg catctttcag cgacggtaat
      961 ttataaattg taccgccaca cagtgttaat agactaaata acgcccattt ctttttcgtg
     1021 agcataacat cccctaagcg atttttaaaa tcactgtttt aaaggcaata agataccgac
     1081 cattcatatg gaatggtttt attaactgcg tatgagttaa aacttattag tggttcagaa
     1141 gatggtttgc ctcttgtttg atttgttgta gtacgttacg cacatgattt atctgattaa
     1201 ttgcatcttc tttttcttta cgtaaccgcg catccagtaa atgattttct ggtaggggtg
     1261 tttcactcat ctggcgataa gggatccacg gattatcatc ttcgtcttca aagcggaacg
     1321 ccgacgcata gtaatcaacc tcttcttcca ggccatatgc cgtcacctgc ggctcatcat
     1381 cgccaaggca gataaggtgc gttaataacg ctttgaatgg catatcaccc tgacctgaaa
     1441 tacaggcttt atgacccagg ccaccctgtt ctttaacgat caaggcatct ttaatatgga
     1501 cctgggtaat atgcggtgcc atcgttttta aggcgtcgat gggatgctca tttgcattga
     1561 tcatgttagc aaaatcaaac agtaaggaaa gagactccat ctcactttct ttgaccagcg
     1621 acaccagctc atgacttttt aaatcttcat gctgctcaat cgtaaaagtc aggccgctgt
     1681 cctgatacgt ttcccgtaca taggcaatgt cgttagcgat aatcgataat acgtcgcgca
     1741 aattaccttc ataacatgga taaaaacgta cggacgttgc cccagttttc aacgcgatgg
     1801 cgacggcttc gtcaatagat gccttatctg aagcgctggt ttcaatatga atatcaaggt
     1861 tcagacggcg ggctttatcc ccaaaggcag agagttcttt atcgtccata ttaccaagag
     1921 aaaaacgctc gccatccaga acatggattt tgacgccacg tagattattt tcactggcga
     1981 tatcaagtaa atcagcaggc aacactcttt ccagccgcat atttaaatga aaggcataag
     2041 catgtaaata aagaggaaga gtgtcgacac gttgtagaat ctttcttgcg ttatttattg
     2101 tcaccataaa acacctacag attaatgatg aggtctacgg cctcaaatcg ccaatattca
     2161 atatctatct ggacaatatt gccttgctgg tcggcgcgat gcttttcaat taagatcgct
     2221 ggcatacccg cagaaccgcc aagatacttg ctgatatcgc ccggcattct gacgggtttg
     2281 aaaatcaatt tttttactac cgtctctttt tggtagcact tttcccagac tgcagaaaat
     2341 gagtggtttt caagttgttc aataaaaccc ggagcaactt caggattaat atatgtttcg
     2401 tgatagaaaa ctttatgtcc ttccagcgcc ccccagccgg tgatgcgata gagttcagtt
     2461 gatggcgtca ctgcaatcag tggcgcgagc gtctcgggaa tatcgctggt acggtttttc
     2521 tcggtaaacc cccaagaagg ctctcgccct tgctcaattg cggcccgctg aaagctcgcc
     2581 gacagctccg gactgtaatt aaatcgcggc tgggtcacga accacccctt acgatcctta
     2641 cggaagattt tggattctgc ctcgaggttc aacaacgcct ggcgcagcgt catacgttta
     2701 atgcccagca gttctcccag ctctctttct gaagggagtt ttccacccgg cgtggtgatg
     2761 ccatctttca accaccgatc cagtttctct tttgcatttt ctacggtgga ttgattctcc
     2821 gccattgttt aatccttttg gtttaaacca gatgaagcat cttacgctta cgccaaaaaa
     2881 gaaagcaatt aacggtgtat gactttgcag ataattgtga caaagctctc tattgacgta
     2941 atcacagact tataagggag atactcgcat tggtgtgcgc tcacgacagg taaaaaaaaa
     3001 cctgccatcg ctggcaggtt ttttatgact aaaaaacgaa attaatcgtc tttcggtgcg
     3061 cggttggcgc ggcgacgatc gttttccgtc aggtgacgtt tacgaatacg gatagaggtc
     3121 ggagtcactt ctaccagttc gtcatcatcg atgaactcca gagcttgttc cagagtcatg
     3181 cggataggcg gaaccagaac aacagcttcg tcagtaccgg aagcacgcat gttggtcagt
     3241 ttcttaccgg tcaggcagtt tacagtcagg tcgttagagc ggctatgaat accgataatc
     3301 tgaccttcgt aaacttctgc accgtgaccg aggaacagct taccgcgatc ctgcagaccg
     3361 aacagcgcga acgcgacagc tttaccctga ccgttagaga tcagtacacc gttctgacgc
     3421 tgacccactt cgcccggacg tacgtcgtcg tagtggctga aggtggagta cagcagacca
     3481 gtaccggaag tcatggtcat gaactcagaa cggaagccaa tcagaccacg gcttgggatc
     3541 acgtagtcga gacgtacgcg gcctttaccg tctggattca tgtttttcag gtcgccttta
     3601 cgttcgccca gcgcctgcat cacagaaccc tgatgctgtt cttcaacgtc cagcgtcacg
     3661 ttttcatacg gctcttgttt acgaccgtct atttcacgga agattacttt cggacgggat
     3721 accgccagtt cgaaaccttc acgacgcatg ttttcgataa gaacagagag gtgcagttca
     3781 ccacgaccag aaacgcggaa cgcatcggcg tcttcggttt cttctacgcg cagcgcaacg
     3841 ttgtgtacca gttctttgtt cagacgatcg aggatctgac gagacgttac gaacttacct
     3901 tctttaccgc agaacggcga ggtgttaacg cagaagaaca tagaaacggt cggctcatca
     3961 acggagagtg ccggcagcgc ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc
     4021 agttcgccca ggcccgtgat cgccacgata tcgccagctt ccgccagatc ggtttcgata
     4081 cgttccagac cgaggtggcc cagcacttta ccgactttcg cgttacgggt tttgccttcg
     4141 ctatcgatga tagtaacctg ctggttcggc ttcactttac cacgcttgat gcggccaatg
     4201 ccgataacgc caacatagct gttgtaatcg agctgagaaa tctgcatctg gaacggaccg
     4261 tcaaggtcaa cgtccggcgc aggaacgtgg tcaacaatcg cctgatacag cggggtcatg
     4321 tcttccgcca tatcttcgtg gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg
     4381 atcgggaagt ccagctgctc gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga
     4441 tccacaaccc aatcaggacg cgcgccaggg cggtcaactt tgttgataac aacaatcggc
     4501 ttcaggccgt aagcaaacgc ttttttggtt acgaagcgcg tttgcggcat cgggccgtca
     4561 aatgcgtcaa ccaccagcag cactgagtct accatggaca ttacacgttc aacttcacca
     4621 ccgaagtcgg cgtgccccgg ggtatcaacg atgttgatac ggtaatcatt ccatttgata
     4681 gcggtgtttt tcgcgaggat ggtaatccca cgctctttct ccaaatcgtt ggagtccatc
     4741 acgcgctctt gggtttcggc acgagagtcg aacgtaccgg attgttggag cagcttgtct
     4801 accagggtgg ttttaccatg gtctacgtgc gcgatgatgg cgatattacg caatttttcg
     4861 atcacaactt tgcctcaggc attagaaata gcgcgttatt gtacacggat taatcgcact
     4921 acaaaacagg atcacaaaca tcctccgcaa acaagtattg cagagtccct ttgtgatcgc
     4981 tttcacggag cataaaaagg gttatccaaa ggtcattgca ccaatatggt gcttaatgtt
     5041 tccattgaag cactatattg gtgcaacatt cacatcatgg tgcagccctt ttgcacgatg
     5101 ttaagcatga taacgccttt taggggcaat ttaaaagttg gcacagattt cgctttatct
     5161 tttttacggc gacacggcca aaataattgc agatttcgtt accacgacga caatgaccaa
     5221 tccaggagag ttaaagtatg tccgctgaac acgtactgac gatgctgaac gagcacgaag
     5281 tgaagtttgt tgatttgcgc ttcaccgata ccaaaggtaa agaacaacac gtcactatcc
     5341 ctgctcatca ggtgaatgct gaattcttcg aagaaggcaa aatgtttgac ggctcctcga
     5401 ttggcggctg gaaaggcatt aacgaatccg acatggtgct gatgccagac gcatccactg
     5461 cagtgattga cccgttcttc gccgactcca ccctgattat ccgttgcgac atccttgaac
     5521 ctggcaccct gcaaggctat gaccgtgacc cgcgctctat tgcgaagcgc gccgaagatt
     5581 acctgcgttc cactggcatt gccgacaccg tactgttcgg gccagaacct gaattcttcc
     5641 tgttcgatga catccgtttc ggatcatcta tctccggttc ccacgttgct atcgacgata
     5701 tcgaaggcgc atggaactcc tccacccaat acgaaggtgg taacaaaggt caccgtccgg
     5761 cagtgaaagg cggttacttc ccggttccgc cggtagactc tgctcaggat attcgttctg
     5821 aaatgtgtct ggtgatggaa cagatgggtc tggtggttga agcccatcac cacgaagtag
     5881 cgactgctgg tcagaacgaa gtggctaccc gcttcaatac catgaccaaa aaagctgacg
     5941 aaattcagat ctacaaatat gtcgtgcata acgtagcgca ccgcttcggt aaaaccgcga
     6001 cctttatgcc aaaaccgatg ttcggtgata acggctccgg tatgcactgc cacatgtctc
     6061 tgtctaaaaa cggcgttaac ctgttcgcag gcgacaaata cgcaggtctg tctgagcagg
     6121 cgctgtacta cattggcggc gtaatcaaac acgctaaagc gattaacgcc ctggcaaacc
     6181 cgaccaccaa ctcttataag cgtctggtcc cgggctacga agcaccggta atgctggctt
     6241 actctgcgcg taaccgttct gcgtctatcc gtattccggt ggtttcttct ccgaaagcac
     6301 gtcgtatcga agtacgtttc ccagatccgg cagctaaccc gtacctgtgc tttgctgccc
     6361 tgctgatggc cggtcttgat ggtatcaaga acaagatcca tccgggcgaa gccatggaca
     6421 aaaacctgta tgacctgccg ccagaagaag cgaaagagat cccacaggtt gcaggctctc
     6481 tggaagaagc actgaacgaa ctggatctgg accgcgagtt cctgaaagcc ggtggcgtgt
     6541 tcacggatga agcgatcgat gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc
     6601 gtatgactcc gcatccggta gagtttgagc tgtactacag cgtctaatag ttgaagttgt
     6661 actacccggc gcacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca
     6721 tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata
     6781 atgcactaaa atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc
     6841 gatgctgggc agatcctcaa ctcgctgatt aacagtattt tgttaattga tgacaatctg
     6901 gcgatccatt acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg
     6961 tttggtacgc cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa
     7021 gaaagtctgg aggcggggca aggctttacc gataacgaag tgacgctggt catcgatggt
     7081 cgctcgcata tcctttctgt gacggcccag cgtatgccgg acggcatgat cctgctggag
     7141 atggcaccga tggataacca gcgccgctta agccaggaac aactacaaca tgctcaacaa
     7201 gtagctgccc gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt
     7261 ttacgtggcg cggcgcagtt actcagcaaa gcgttacctg acccatcact actcgaatat
     7321 accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatt tggtcgaccg tctgttgggg
     7381 ccgcagctgc ccggtacgcg cgttaccgaa agtattcaca aagtggctga acgcgtggta
     7441 acgctggtgt cgatggaact gccggacaac gtgcggttga ttcgtgatta cgatcccagc
     7501 ctgccggaac tggcgcacga cccggatcaa attgaacagg tcttgctgaa tattgtgcgc
     7561 aatgcgctac aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac ccgcaccgcg
     7621 tttcaactga ccttacacgg cgagcgctac cggctggcgg cgcggattga tgtggaagat
     7681 aacgggccgg gcattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc
     7741 cgcgaaggtg gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca
     7801 ggcaaaattg aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc
     7861 aggaaataaa ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt
     7921 ccatccgttg ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acgacatttg
     7981 agaacggcgc ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag
     8041 atatccgtat gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc
     8101 caatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct
     8161 atcaacaagg ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gcagtggcgc
     8221 tggttgagcg cgctatcagt cattaccagg aacagcagca gccgcgtaat attcagctta
     8281 acggcccaac gaccgatatc atcggcgaag cgcaagccat gcaggacgtg ttccgtatta
     8341 tcggtcggct ttcgcgttct tctattagcg tgctgattaa cggcgaatcc ggcaccggta
     8401 aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg caccaaagcg ccgtttatcg
     8461 cgctgaatat ggcagctatc ccaaaagatt tgatcgaatc agaactgttt ggccacgaga
     8521 aaggcgcgtt tactggcgcg aataccattc gtcaggggcg ttttgaacag gccgatggcg
     8581 gcacgttatt tctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc
     8641 gcgtgctggc agacggtcag ttttatcgcg ttggcggcta tgcgccggtg aaagtggatg
     8701 tgcggattat cgctgccact caccagaatc ttgaacagcg ggtgcaggaa ggtaagttcc
     8761 gtgaggatct gttccaccgc ctgaacgtta tccgcgttca tctgccgccg ctgcgcgaac
     8821 gtcgggaaga tattccccgt ctggcgcgcc attttttaca ggttgccgcg cgcgaactgg
     8881 gcgtagaagc gaagttgctg catccggaaa ccgaagctgc tctgacgcgt ctggcgtggc
     8941 caggcaacgt gcgccagctg gaaaacacct gccgctggct aacggtgatg gccgccgggc
     9001 aggaagtgtt gattcaggat ttgcccggcg aactgtttga atcaacggtt gcggagagta
     9061 cttcgcaaat gcaaccggac agttgggcga cacttttagc gcagtgggca gacagagcgc
     9121 tgcgttccgg tcatcaaaat ctgctttccg aagcgcagcc agagctggag cggacgttac
     9181 tgacgaccgc gttgcgacat acgcaggggc ataaacagga agcggcgcgg ctactcggct
     9241 ggggccgcaa cactctgacg cgtaagttaa aagagctggg gatggagtga ttcacggctt
     9301 gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttgcgcttta ctgttccgat
     9361 aagttcagta tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta
     9421 atctggtatc gagtggcgca gttgacagcc acacaccgca aactgccgtt gctgccgtgc
     9481 tgtgtgccgc gatgattggg ctgtttagct gaagctcgtt gtttatgccg gatgcggaga
     9541 aagtcgtagg cctgataaga cgcgaccagc gtcgcatcag gcatcatgct cagatgcctg
     9601 atgcggcgta aacgccttat ccggcctaca aaagcaataa tttcaacagg ttttgttgcc
     9661 cagcgcatca ggcaatcttg ttctcagcac attgatggcg ctttcgtttt ctactttgta
     9721 aacgaagcgc cattcactct ttaaatcacc cgtgagaact gctgcatccg cgctttctgg
     9781 cgcagatagg tatcaaagca catgcaaatg ttgcggatca gcaagcgacc tttcgccgtc
     9841 acctgtattc ccttctcatc cacatccacc agcccatctt ttgctaacgg ggcgagcagc
     9901 ttgagatctt ccgcaaagta atcagcgaag tgcaaatccc actgtttctc aagaggggcg
     9961 taatccagac ggaagttgca gatgagcgac ttaatcacat cgcggcgaat acagtcatca
    10021 cgcgttagcg caataccacg ccacagcgca ttgccttgtt catacacttg ctgatagtac
    10081 tgcttcaact ctttctggtt ctgcgcgtag cagtcgccaa tcatgctgat ggcggaaacg
    10141 cccatcccca gcagatcggt atcgccctga gtggtgtagc cctggaagtt acgatgcagc
    10201 acgccttcac gctgggccac cgccagctcg tcatcaggac gggcaaagtg atccataccg
    10261 ataaactgat agcccgattg cgtcaggaag gcgatggttt cctgcaggat atcgagtttt
    10321 tgctgcggac tcggcaggtc agcatctttg attttgcgct gagcagcaaa aatggtcggc
    10381 agatgcgcgt agttaaaaac gctcagacga tcggggttca gctccgccac acgtttcagg
    10441 gtaaaggcga aactctccgg cgtctgtttc ggcaggccgt aaatcaggtc gatgttggtg
    10501 gaggtaaaac caatctcacg cgcatggtta agcagtgcaa agatgaactc ttcatcctgc
    10561 tcgcggttaa ccagacgttg cacttctttg ttgaagtcct gcacgcccat gctcaggcga
    10621 ttaaagcctt cggcgcgtaa atgatcgagt acatccagtt cgatttcccg cggatcgact
    10681 tcgatcgaaa tctccgcatc ggtattgaaa tggaagtttt cgcgcagcag cttcatcaga
    10741 cggctgattt gcgctttatt cagatacgtc ggcgttccgc cgccccagtg caattggctg
    10801 acgtgacgcc cggcgaacag cggtgcacga tggacgattt cttgctccag cgcgtccaga
    10861 tactgatcgg ccttgtgctg ctggcgagta acaatcttat tgcaaccgca gaagtaacaa
    10921 agcttatggc agaacgggat atgtacgtag agagataatg gacgctcagg atagcgcgcc
    10981 acggcttgta aaaacgcctg ttcgccgaag tcttctgaaa actccagcgc ggtcgggtac
    11041 gaggtgtatc gtggcccgga atagttatat ttctggatca gggccagatc ccagtcgatt
    11101 tgctgtacag acatgctcac tccttccgat ggcgtctctg gcggctacgg cgtaccggcg
    11161 gatgcccgtt tcgggtcatc agccggttgc gcagccactt ttgtcgccgc gacaaccgct
    11221 gtagtttaac gaataaccac accagataac atataaccgg aagggttatc agcagaacgg
    11281 gaaggcccac ggcggcaatc cgttagttgc cccgcagcag acgcatcatg tcttcttgct
    11341 tctcgtcttc ttcctcttct tcgtcatcgt cataagagag gccgagtttc tgcatcaact
    11401 catcaatacg atccagttta gcatccaccc aggattgctc ttcggcgctc agggtttcgc
    11461 ctgcttccag acgttccagc agcgcatcca gacactcatc cgtttccagt aattccaact
    11521 ccgcctgcgg tgaaagcata ggtttctcac tcttcggttt gtgctgtttg gtgacttttt
    11581 cagtcactcc caatggaata ggggttttac tgccaatacg tggatctttt ggtgcgttct
    11641 ggcctttgct gcctgacgtg gtgtttccgc ccgctgcgcg gctgcccggc gcatgaccac
    11701 gacgtttttt ctgacgctta cggtcacgag cttcctgatc cagctcctcg cgtgtttttc
    11761 gacgcgcttt tgcatgacct ttgctgcgtg agtttgaaga tgatggtttc ataatggttc
    11821 gtcttttagc cgttttattc agtatagatt tgcggcggaa tctaacagaa agcaagcaaa
    11881 gaaaaaaggc gacagattac tctgtcgcct tttttcctga ctcataaccc ttaacgggtc
    11941 ttacaatcct tgcaggccgc caacacaccc tgtttttcca ttagccggaa caatccgtgt
    12001 tgattccatt tccgtttaat tacgtctccg gacgtttgtc ttcctgacaa tcctctgtct
    12061 tcgcctcctg gcgctcctga ccctcatcct gagtcattgt tcctgttagc gtcctcgctc
    12121 tatgggtgct actttacgcc tttgctttaa acaaacaatc aaccgccatc agtatttgca
    12181 cattttcaga gttttatata aaagcaattc actgatttat atagcttatt tgacatgcat
    12241 cgcagaattt tctctgctat ttcgcataaa gactatcggc aatgtctcac aaagcgtatg
    12301 gcttttactt acaacgctca cgccgaaaaa tccgcctttt attatggttc aggtataatc
    12361 gccacaaagc tttgactgac ggagacgacc gctttgacta atttgaatta tcaacagacg
    12421 cattttgtga tgagtgcgcc tgatattcgc cacctacctt ccgataccgg aattgaagtg
    12481 gcttttgcag gccgttccaa cgcaggtaaa tccagcgcgc tgaacacgct gactaaccag
    12541 aaaagcctgg ctcgtacctc aaaaacccca gggcgcaccc agcttatcaa cctgtttgaa
    12601 gtggctgacg gcaagcgtct ggttgacttg cctgggtacg gttatgcgga agtcccggaa
    12661 gagatgaagc gcaaatggca gcgtgcgctc ggcgaatacc tcgaaaaacg tcagagcctg
    12721 caaggtctgg tggtgctaat ggatattcgc catccgctga aagatttgga tcagcagatg
    12781 attgagtggg cggtagacag caatatcgcc gttctggtgc tgctgaccaa agcggacaaa
    12841 ctggcaagcg gcgcacgtaa agcgctattg aatatggtgc gtgaagctgt actggcgttt
    12901 aacggtgatg tgcaggttga aacgttttct tcgttgaaga aacaaggcgt agacaagctg
    12961 aggcagaaac tggatacctg gtttagcgag atgcagcctg tagaagaaac gcaggacggc
    13021 gaataatttt cttgccttaa tgcttgtgcc ggatgtggcg tatccggccc gtaaattcaa
    13081 tagattacaa ttctaccgtt ggcctgataa acatagcgca tcaggcatta cggatctttt
    13141 ctttcgccca ataaaaaacg ccccagtcat tactgactgg ggcggctaaa atattcagcc
    13201 aaatccgatt acgtgaagta aaaggtctga aagatagaac atcttacctc tgtaccctac
    13261 gcgactaact ttactctttt ttgttcagtt cagaaagcac tttttgtaat tttttttcag
    13321 ttgttgcata ggaatttcta atagccatca caaaacgtga cagcttatgt tgcttactta
    13381 cgaaaaaagg gcatgttcag gcgaatctta gtgcgcctga tcccagtttt cgccactccc
    13441 cacttccacc agcaacggca catccagacg ggtacagttt tccatcagtt gatgaatctg
    13501 cttcgcgacg gcatcaacat catctttatg aacttcaaat accagttcat cgtgtacctg
    13561 catgatcata cgtacacgcg gttgctcagc ctgtaaccac gcatcaacgg caatcatcgc
    13621 ccgtttgata atgtcggcgg cggttccctg cattggcgcg ttaatggctg cacgttcagc
    13681 cgctgcacga cgagcaccat tgctggattt gatatccggc agatacagac ggcgtccgtc
    13741 cagcgtttca acgtagccca gctctttcgc ctgagcacgg gtgcgttcca tatactccag
    13801 cacgccaggg tagcgttcga agtaaaggtc catgtacttc tgcgcttctt tacgtggaat
    13861 gttcaattgc cgcgccagac cgaaagcact catgccataa atcagaccaa agttgatcgc
    13921 tttcgcgcta cggcgttgct cgctggtgac ggtttccagt ggcaaaccaa acacttctgc
    13981 cgccgttgcc cggtggatat cttttccttc cgcgaatgcg gtcagcaagc ctttatcacg
    14041 cgagagatgc gccataatgc gcagttcaat ctgcgagtag tccgctgaga caatcacata
    14101 atcctctggc gcaataaacg cctggcggat acgacgacct tcttcgttac gcaccggaat
    14161 gttttgcagg ttaggatcgg ttgacgataa acgtcccgtt gcagttactg cctggtgata
    14221 agaggtatgc acacgcccgg ttttcgggtt gatcatcagc ggcagcttgt cggtgtaagt
    14281 cgacttcagc ttcgccagac cacgatactc cagaatcact tttggcaacg gatagtccag
    14341 cgccagttct tccagtacct cttccgacgt tgacggcgcg ccacccggcg ttttcttcag
    14401 cggtttaata ccctgttttt caaacagaat ggtttgcaac tgcttggtgg aagaaaggtt
    14461 aaattcttca cctgcaattt catgcgcttt cttttccagc tcagccagac gcaaggtgag
    14521 ctcttcagaa tgattgtgca gcactttcgg atcgatcttc acaccgttac gttcaatgcg
    14581 tgaaagcacc ggcaccagcg gcatttcgat attctcgaag acgttcaacg gccctttgtg
    14641 tttctgcaga tccggccaca ttttcagatg caactgcaag gtgacatctg catcttcggc
    14701 ggcgtaacgt ccggcctctt cgagggcaat ctggttaaag gtcagttgat ttttgccttt
    14761 acccgcaatc tcttcaaaag tgatggtttt gtgcttcaac caacgttccg cgaggctgtc
    14821 catatcgtga cgcccggcaa cgctattgag aatgtaggac tccagcatgg tatcaaacgc
    14881 aatcccacgc agttcaatgc cgtaattcgc cagaataccg cgatcgtatt tcaggttttg
    14941 cccgaccttc agcgcctttt catcttccag cagcggtttt agcaactcga gtgcacgctc
    15001 gtgagagatt tgatcgggcg catcaagata atcatgagca accggaatat atgccgctac
    15061 gcctggctcg atagcaaaag aaagcccgac caggttagca gagatgttat caaggctgtc
    15121 ggtttcggta tcaaatgcaa ataccggcgc tttttccagc ttcgcaatcc acgctttcag
    15181 tgtttcttca tcaaggatag tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc
    15241 tggcgcttcg tctgcaacac tggtttcctg aggcttcgcg gctgtttttg cccctttggc
    15301 ctgtaaccat ttgcccactt cgacatcagc agtccagcgt ttgaactcat actttttgaa
    15361 cagccccaac aactcttccg ctgccggttg ctgcacttcc agttgttcac aggtcagctc
    15421 cagttcaaca tcggttttaa tcgtcgccaa ctggtatgag agataagcaa cttctttgtt
    15481 ttgctcgagc ttcgctgcca ttgttttcgc gccacggaag ctcaacccag caattttttc
    15541 tggctcggca tacagcgtat ccagcccgcc aagaccttgc agcaatgcct gcgcggtttt
    15601 ttcaccgacg cccggtacgc caggaatgtt atcagaggag tcacccatca gcgccaggaa
    15661 gtcgatgatc aattctggcg gcacgccgta cttattcacc acctcttccg gtccgaggat
    15721 ggtattcgtc atggtattga taagcgtaat atttggcgtc accagctgcg ccatatcttt
    15781 atcgccagtg ctaatcagca ccggacgccc ggctttttcg gcttcgcgcg ccagagtacc
    15841 gataacgtcg tccgcttcta cgccagaaac cgccagcagc ggcagtccca tcgctttaac
    15901 catcgcgtgc agaggttcga tttgtgctcg cagatcgtcc ggcattggcg ggcgatgtga
    15961 tttgtaatgt tcaaacagtt catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc
    16021 tgcatgcgtc ggtttatatt gcatgatcag actgcgcagc atgttgagga caccatacat
    16081 cgcaccggtc ggctcgcctg cgctgttagt cagcggggga aacgcgtgat atgcgcgata
    16141 aagataagat gaaccatcta caaggataag tggattttgg gggatctgaa ccataatgtc
    16201 cgtgcctgtt tatcagatta tgggtaaagg atgccacaga caggatgaaa acatgagttt
    16261 ttcaccgctt ttcgtgaaaa tcttttgtag atctgctgga tcgctcgcaa atcgtcatgt
    16321 ggataacttt gtgcataaat tattt
//
LOCUS       DAZSIO010000085        16096 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZSIO010000085 DAZSIO010000000
VERSION     DAZSIO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.085"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..588
                     /locus_tag="ACHX5T_002483"
     CDS             <1..588
                     /locus_tag="ACHX5T_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFR8467644.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            771..965
                     /gene="bfd"
                     /locus_tag="ACHX5T_002484"
     CDS             771..965
                     /gene="bfd"
                     /locus_tag="ACHX5T_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFR8467645.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            1038..1514
                     /gene="bfr"
                     /locus_tag="ACHX5T_002485"
     CDS             1038..1514
                     /gene="bfr"
                     /locus_tag="ACHX5T_002485"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFR8467646.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1511..1978)
                     /locus_tag="ACHX5T_002486"
                     /pseudo
     CDS             complement(1511..1978)
                     /locus_tag="ACHX5T_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
     gene            2357..2668
                     /gene="rpsJ"
                     /locus_tag="ACHX5T_002487"
     CDS             2357..2668
                     /gene="rpsJ"
                     /locus_tag="ACHX5T_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR8467647.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2701..3330
                     /gene="rplC"
                     /locus_tag="ACHX5T_002488"
     CDS             2701..3330
                     /gene="rplC"
                     /locus_tag="ACHX5T_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR8467648.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3341..3946
                     /gene="rplD"
                     /locus_tag="ACHX5T_002489"
     CDS             3341..3946
                     /gene="rplD"
                     /locus_tag="ACHX5T_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR8467649.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3943..4245
                     /gene="rplW"
                     /locus_tag="ACHX5T_002490"
     CDS             3943..4245
                     /gene="rplW"
                     /locus_tag="ACHX5T_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR8467650.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4263..5084
                     /gene="rplB"
                     /locus_tag="ACHX5T_002491"
     CDS             4263..5084
                     /gene="rplB"
                     /locus_tag="ACHX5T_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR8467651.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            5101..5379
                     /gene="rpsS"
                     /locus_tag="ACHX5T_002492"
     CDS             5101..5379
                     /gene="rpsS"
                     /locus_tag="ACHX5T_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR8467652.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5394..5726
                     /gene="rplV"
                     /locus_tag="ACHX5T_002493"
     CDS             5394..5726
                     /gene="rplV"
                     /locus_tag="ACHX5T_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR8467653.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5744..6445
                     /gene="rpsC"
                     /locus_tag="ACHX5T_002494"
     CDS             5744..6445
                     /gene="rpsC"
                     /locus_tag="ACHX5T_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR8467654.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6458..6868
                     /gene="rplP"
                     /locus_tag="ACHX5T_002495"
     CDS             6458..6868
                     /gene="rplP"
                     /locus_tag="ACHX5T_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR8467655.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6868..7059
                     /gene="rpmC"
                     /locus_tag="ACHX5T_002496"
     CDS             6868..7059
                     /gene="rpmC"
                     /locus_tag="ACHX5T_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR8467656.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            7059..7313
                     /gene="rpsQ"
                     /locus_tag="ACHX5T_002497"
     CDS             7059..7313
                     /gene="rpsQ"
                     /locus_tag="ACHX5T_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR8467657.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7478..7849
                     /gene="rplN"
                     /locus_tag="ACHX5T_002498"
     CDS             7478..7849
                     /gene="rplN"
                     /locus_tag="ACHX5T_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR8467658.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7860..8174
                     /gene="rplX"
                     /locus_tag="ACHX5T_002499"
     CDS             7860..8174
                     /gene="rplX"
                     /locus_tag="ACHX5T_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR8467659.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8189..8728
                     /gene="rplE"
                     /locus_tag="ACHX5T_002500"
     CDS             8189..8728
                     /gene="rplE"
                     /locus_tag="ACHX5T_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR8467660.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8743..9048
                     /gene="rpsN"
                     /locus_tag="ACHX5T_002501"
     CDS             8743..9048
                     /gene="rpsN"
                     /locus_tag="ACHX5T_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR8467661.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            9082..9474
                     /gene="rpsH"
                     /locus_tag="ACHX5T_002502"
     CDS             9082..9474
                     /gene="rpsH"
                     /locus_tag="ACHX5T_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR8467662.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9487..10020
                     /gene="rplF"
                     /locus_tag="ACHX5T_002503"
     CDS             9487..10020
                     /gene="rplF"
                     /locus_tag="ACHX5T_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR8467663.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            10030..10383
                     /gene="rplR"
                     /locus_tag="ACHX5T_002504"
     CDS             10030..10383
                     /gene="rplR"
                     /locus_tag="ACHX5T_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR8467664.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10398..10901
                     /gene="rpsE"
                     /locus_tag="ACHX5T_002505"
     CDS             10398..10901
                     /gene="rpsE"
                     /locus_tag="ACHX5T_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR8467665.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10905..11084
                     /gene="rpmD"
                     /locus_tag="ACHX5T_002506"
     CDS             10905..11084
                     /gene="rpmD"
                     /locus_tag="ACHX5T_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR8467666.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            11088..11522
                     /gene="rplO"
                     /locus_tag="ACHX5T_002507"
     CDS             11088..11522
                     /gene="rplO"
                     /locus_tag="ACHX5T_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR8467667.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11530..12861
                     /gene="secY"
                     /locus_tag="ACHX5T_002508"
     CDS             11530..12861
                     /gene="secY"
                     /locus_tag="ACHX5T_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR8467668.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12893..13009
                     /gene="rpmJ"
                     /locus_tag="ACHX5T_002509"
     CDS             12893..13009
                     /gene="rpmJ"
                     /locus_tag="ACHX5T_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR8467669.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13156..13512
                     /gene="rpsM"
                     /locus_tag="ACHX5T_002510"
     CDS             13156..13512
                     /gene="rpsM"
                     /locus_tag="ACHX5T_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR8467670.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13529..13918
                     /gene="rpsK"
                     /locus_tag="ACHX5T_002511"
     CDS             13529..13918
                     /gene="rpsK"
                     /locus_tag="ACHX5T_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR8467671.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13952..14572
                     /gene="rpsD"
                     /locus_tag="ACHX5T_002512"
     CDS             13952..14572
                     /gene="rpsD"
                     /locus_tag="ACHX5T_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR8467672.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14598..15587
                     /gene="rpoA"
                     /locus_tag="ACHX5T_002513"
     CDS             14598..15587
                     /gene="rpoA"
                     /locus_tag="ACHX5T_002513"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR8467673.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15628..16011
                     /gene="rplQ"
                     /locus_tag="ACHX5T_002514"
     CDS             15628..16011
                     /gene="rplQ"
                     /locus_tag="ACHX5T_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR8467674.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4141 a         3783 c         4292 g         3880 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggccgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgtt gctaaagttc tgggctaatt acacgttaat
      601 tagttttgaa ttgaaaaggg cgcttcggcg ccctttttgc tgcccgtaat ttgagctcga
      661 aatatttagt cgtaattttg ctcgctgaac tatggcgcaa ataaaactgt gctattgtaa
      721 tcataaccat tctcatttac actttgtgca gaatttgaac gggagcaatc atgtacgttt
      781 gtttgtgtaa tggtgtaagt gacaaaaaaa ttcgccaggc agtccgtcag tttcatccgc
      841 aatctttcca acaattgcgt aagtttattc ctgtgggaaa tcaatgcggt aagtgcattc
      901 gcgccgcgcg cgaagtgatg caggacgagt tgatgcagat gccagaattt aaagagattg
      961 cctgaagccc acgcttattt tttgacatcc tgctagcccc atctacgctc taatagtgga
     1021 agcggaggga ctataaaatg aaaggtgatg ttaaaatcat aaattatctc aataaactat
     1081 tgggaaatga gcttgtcgca atcaatcagt attttctcca tgcccggatg tttaaaaact
     1141 ggggcctgat gcgtctcaat gacgtcgaat accatgaatc catcgatgag atgaaacacg
     1201 ctgataaata tatcgagcgt attttgtttt tagaaggaat ccccaactta caggatttag
     1261 gcaaactggg gattggtgaa gatgtcgagg agatgctgca atctgacctg cgattagaac
     1321 tggaaggcgc gaaggatctg cgtgaggcta tcgcatatgc cgacagcgtt catgactatg
     1381 ttagccgcga tatgatgatc gagatccttg ctgatgaaga aggtcatatc gactggctcg
     1441 aaactgaact ggatctcatc ggtaaaatcg gcctacaaaa ttacctgcaa tcacaaatta
     1501 aagttaaaga ttaacggcct atcagcaaac tatcccagcc tataacgaaa cccgcagccg
     1561 ccagaaatgg cccaaaaggc agcgggtttt ttaatgattc ttttcctctc atgagcagac
     1621 caataactac ggctccgcaa gcgaatgagg cagcaaggaa aaccagtcgt ggtaagaagg
     1681 cccatgaatg ctaggcgcca agggcggcga gaaacttcac atcaccatag cctaatcctt
     1741 ctttatgtcg cagtatgcga tacccccagt aaatgacagc gaatgtgccg taaccaacaa
     1801 ttgcccccca taacgcatcg gctaaaccat caggatgaca aacttgatag aacaaaagac
     1861 ctgaccagag taacgggcac gtaaatctgt caggtaacaa gccatgcttt gcatcccaga
     1921 aaaaaagtag ggctgataaa caagcgtaca aaattagaaa gggaagggta gtgtccataa
     1981 aacatcctct gtaattggag tatcaccatc gaataactcc ggatgctcag caacagagag
     2041 agcgctgatt tgttaacctg attccggtga aaaacgctat cttcacaacc ttacaaattt
     2101 cgtggaatgc agctcgcaat ctgcgcgttg cgattaattt ctacccgatg cttgcgtcag
     2161 gctcagattt gcgtataatg cgcgggcttg tcgtagttga cagcaggttc aatctgaacc
     2221 tcagttagcc gaatttggct acctaacaat gctcccaatc ggggagctac gtaagaacgg
     2281 ttacactctc ccatcaatcg taatgggtct gaggagtaat cattttcgtt tataaaataa
     2341 ttggagctct ggtctcatgc agaaccaaag aatccgtatc cgcctgaaag cgtttgatca
     2401 tcgtctgatc gatcaagcaa ccgcggaaat cgtcgagact gccaagcgca ctggtgcgca
     2461 ggtccgtggt ccgatcccgc tgccgacacg caaagagcgc ttcactgttc tgatctcccc
     2521 gcacgtcaac aaagacgcgc gcgatcagta cgaaatccgt actcacttgc gtctggttga
     2581 catcgttgag ccaaccgaga aaaccgttga tgctctgatg cgtctggatc tggctgccgg
     2641 tgtagacgtg cagatcagcc tgggttaatc aggtcattga gcgattgaga ggttgaaaca
     2701 atgattggtt tagtcggtaa aaaagtgggt atgacccgta tcttcacaga agacggcgtt
     2761 tctatcccag taaccgtaat cgaagttgaa gcaaaccgcg ttactcaggt taaagacctg
     2821 gctaacgatg gctaccgtgc tattcaggtg accaccggtg ctaaaaaagc taaccgtgtg
     2881 accaagcctg aagctggcca cttcgctaaa gctggcgtag aagctggccg tggtctgtgg
     2941 gaattccgcc tggctgaagg cgaagagttc actgtaggtc agagcattag cgttgaactg
     3001 tttgctgacg ttaaaaaagt tgacgtaact ggcacctcta aaggtaaagg tttcgcaggt
     3061 accgttaagc gctggaactt ccgtacccag gacgctactc acggtaactc cttgtctcac
     3121 cgcgttccgg gttctatcgg tcagaaccag actccgggca aagtgttcaa aggcaagaaa
     3181 atggcaggtc agatgggtaa cgaacgtgta accgttcaga gccttgacgt agtacgcgtt
     3241 gacgctgagc gcaacctgct gctggttaaa ggtgctgtcc cgggtgcaac cggtagcgac
     3301 ctgatcgtta aaccagctgt gaaggcgtaa ggagatagca atggaattag tattgaaaga
     3361 cgcgcagagc gcgctgactg tttccgaaac taccttcggt cgtgatttca acgaagcgct
     3421 ggttcaccag gttgttgttg cttatgcagc tggtgctcgt cagggtactc gtgctcagaa
     3481 gactcgtgct gaagtaactg gttccggtaa aaaaccgtgg cgccagaaag gcaccggccg
     3541 tgcgcgttct ggttctatca agagcccgat ctggcgttct ggtggcgtga cctttgctgc
     3601 tcgtccgcag gaccacagtc aaaaagttaa caagaagatg taccgcggcg cgctgaaaag
     3661 catcctgtcc gaactggtac gtcaggatcg tctgatcgtt gtcgagaagt tctctgtaga
     3721 agcgccgaaa actaagctgc tggcacagaa actgaaagac atggctctgg aagatgtgct
     3781 gatcatcacc ggtgagctgg acgaaaacct gttcctggct gcgcgcaacc tgcagaaggt
     3841 tgacgtacgc gatgcaactg gtatcgaccc ggttagcctg atcgccttcg acaaagtcgt
     3901 aatgactgct gatgctgtta agcaagttga ggagatgctg gcatgattcg tgaagaacgt
     3961 ctgctgaagg tgctgcgtgc accgcacgtt tctgaaaaag cgtctactgc gatggaaaaa
     4021 tccaacacca tcgtactcaa agttgctaaa gacgcgacca aagcagaaat caaagctgct
     4081 gtgcagaaac tgtttgaagt cgaagtcgaa gtcgttaaca ccctggtagt taaagggaaa
     4141 gttaaacgtc acggacagcg tatcggtcgt cgtagcgact ggaaaaaagc ttacgtcacc
     4201 ctgaaagaag gccagaatct ggacttcgtt ggcggcgctg agtaagtcgg aggagtaata
     4261 caatggcagt tgttaaatgt aaaccgacat ctccgggtcg tcgccacgta gttaaagtgg
     4321 ttaaccctga gctgcacaag ggcaaacctt ttgctccgtt gctggaaaaa aacagcaaat
     4381 ccggtggtcg taacaacaat ggccgtatca ccactcgtca tatcggtggt ggccacaagc
     4441 aggcttaccg tattgttgac ttcaaacgca acaaagacgg tatcccggca gttgttgaac
     4501 gtcttgagta cgatccgaac cgttccgcga acatcgcgct ggttctgtac aaagacggtg
     4561 aacgccgtta catcctggcc cctaaaggcc tgaaagctgg cgaccagatt cagtctggcg
     4621 ttgatgctgc aatcaaacca ggtaacaccc tgccgatgcg caacatcccg gttggttcta
     4681 ctgttcataa cgtagaaatg aaaccaggta aaggcggtca gctggcacgt tccgctggta
     4741 cttacgttca gatcgttgct cgtgatggtg cttatgtcac cctgcgtctg cgttctggtg
     4801 aaatgcgtaa agtagaagca gactgccgtg caactctggg cgaagttggc aatgctgagc
     4861 atatgctgcg cgttctgggt aaagcaggtg ctgcacgctg gcgtggtgtt cgtccgaccg
     4921 ttcgcggtac cgcgatgaac ccggtagacc acccacatgg tggtggtgaa ggtcgtaact
     4981 ttggtaagca cccggtaact ccgtggggcg ttcagaccaa aggtaagaag acccgcagca
     5041 acaagcgtac tgataaattc atcgtacgtc gccgtagcaa ataattttag aggataagcc
     5101 atgccacgtt ctctcaagaa aggtcctttt attgacctgc acttgctgaa gaaggtagag
     5161 aaagcggtgg aaagcggaga caagaagccc ctgcgcactt ggtcccgtcg ttcaacgatc
     5221 tttcctaaca tgatcggttt gaccatcgct gtccataatg gtcgtcagca cgttccggta
     5281 tttgtaaccg acgaaatggt cggtcacaaa ctgggtgaat tcgcaccgac tcgtacttat
     5341 cgcggccacg ctgctgataa aaaagcgaag aagaaataag gtaggaggaa gagatggaaa
     5401 ctattgctaa acatcgccat gctcgttctt ctgctcagaa ggttcgcctt gttgctgacc
     5461 tgattcgcgg taagaaagtg tcgcaggctc tggatatttt gacctacacc aacaagaaag
     5521 cggctgtaca ggtcaagaaa gttctggaat ctgccattgc taacgctgaa cacaacgatg
     5581 gcgctgacat tgacgatctg aaagttacga aaattttcgt agacgaaggc ccgagcatga
     5641 agcgcattat gccgcgtgca aaaggtcgtg cagatcgcat cctgaagcgc accagccaca
     5701 tcactgtggt tgtgtccgat cgctgagact ctggagacta gcaatgggtc agaaagtaca
     5761 tcctaatggt attcgcttgg gtattgtaaa accatggaac tctacttggt ttgcgaacac
     5821 caaagaattc gctgacaacc tggacagcga ttttaaagta cgtcagtacc tgactaagga
     5881 actggctaaa gcgtccgtat ctcgtatcgt tatcgagcgt ccggctaaga gcatccgtgt
     5941 aaccattcac actgctcgcc cgggtatcgt tatcggtaaa aaaggtgaag acgtagaaaa
     6001 actgcgtaag gtcgtagcgg acatcgctgg cgttcctgca cagatcaaca tcgccgaagt
     6061 tcgtaagcct gaactggacg caaaactggt tgctgacagc atcacttctc agctggaacg
     6121 tcgcgttatg ttccgtcgtg ctatgaagcg tgctgtacag aacgcaatgc gtctgggcgc
     6181 taaaggtatt aaagttgaag ttagcggccg tctgggcggc gcggaaatcg cacgtaccga
     6241 atggtaccgc gaaggtcgcg taccgctgca cactctgcgt gctgacatcg actacaacac
     6301 ctctgaagcg cacaccactt acggtgtaat cggcgttaaa gtgtggatct tcaaaggcga
     6361 gatcctgggt ggtatggctg ctgttgaaca accggaaaaa ccggctgctc agcctaaaaa
     6421 gcagcagcgt aaaggccgta aataaggagc gtcgctgatg ttacaaccaa agcgtacaaa
     6481 attccgtaaa atgcacaaag gccgtaaccg cggtctggcg cagggtacgg atgttagctt
     6541 cggcagcttc ggtctgaaag ctgttggccg tggtcgtctg actgcccgtc agatcgaagc
     6601 agcacgtcgt gctatgaccc gtgcagttaa gcgtcaaggt aagatctgga tccgtgtgtt
     6661 cccggacaaa ccgatcactg aaaagccgct ggcagtgcgt atgggtaaag gtaaaggtaa
     6721 cgtggagtat tgggttgcct tgattcagcc gggtaaagtc ctgtatgaaa tggacggtgt
     6781 tccggaagag ctggcccgtg aagcattcaa gctggcagca gcgaaactgc cgattaaaac
     6841 cacctttgta actaagacgg tgatgtaatg aaagcaaaag agctgcgtga gaagagcatt
     6901 gaagagctga acaccgagct gctgaacctg ctgcgtgagc agttcaacct gcgtatgcag
     6961 gctgcaagtg gccagctgca acagtctcac ctgttgaagc aagtgcgtcg cgatgtcgca
     7021 cgcgttaaga ctttactgaa cgagaaggcg ggtgcgtaat gaccgataaa atccgtactc
     7081 tgcaaggtcg cgttgttagc gacaaaatgg agaaatccat tgttgttgct atcgaacgtt
     7141 ttgtgaaaca cccgatctac ggaaaattca tcaagcgtac gaccaaactg cacgtacatg
     7201 acgagaacaa cgaatgcggt atcggtgacg tggttgaaat ccgcgaatgc cgtccgctgt
     7261 ccaagactaa atcctggacg ctggttcgcg ttgtagagaa agcggttctg taatacagta
     7321 cactctctcg atacgaataa acggctcaga aatgagccgt ttattttttc tacccatatc
     7381 cttgaagcgg tgttataatg ccgcgccctc gatatgggga tttttaacga cctgattttc
     7441 gggtctcagt agtagttgac attagcggag cactaaaatg atccaagaac agactatgct
     7501 gaacgtcgcc gacaactccg gtgcacgtcg cgtaatgtgt atcaaggttc tgggtggctc
     7561 gcaccgtcgc tacgcaggcg taggcgacat catcaagatc accatcaaag aagcaattcc
     7621 gcgtggtaag gtcaaaaaag gtgatgtgct gaaggcggta gtggtgcgca ccaagaaggg
     7681 tgttcgtcgc ccggacggtt ctgtcattcg cttcgatggt aatgcttgtg ttcttctgaa
     7741 caacaacagc gagcagccta tcggtacgcg tatttttggg ccggtaactc gtgagcttcg
     7801 tagtgagaag ttcatgaaaa ttatctctct ggcaccagaa gtactctaag gagcgaatca
     7861 tggcagcgaa aatccgtcgt gatgacgaag ttatcgtgtt aaccggtaaa gataaaggta
     7921 aacgcggtaa agttaagaat gtcctgtctt ccggcaaggt cattattgaa ggtatcaacc
     7981 tggttaagaa acatcagaag ccggttccgg ccctgaacca accgggtggc atcgttgaaa
     8041 aagaagccgc tattcaggtt tccaacgtag caatcttcaa tgcggcaacc ggcaaggctg
     8101 accgtgtagg ctttagattc gaagacggca aaaaagtccg tttcttcaag tctaacagcg
     8161 aaactatcaa gtaatttgga gtagtacgat ggcgaaactg catgattact acaaagacga
     8221 agtagttaaa aaactcatga ctgagtttaa ctacaattct gtcatgcaag tccctcgggt
     8281 cgagaagatc accctgaaca tgggtgttgg tgaagcgatc gctgacaaaa aactgctgga
     8341 taacgcagca gcagacctgg cagcaatctc cggtcaaaaa ccgctgatca ccaaagcacg
     8401 caaatctgtt gcaggcttca aaatccgtca gggctatccg atcggctgta aagtaactct
     8461 gcgtggcgaa cgcatgtggg agttctttga gcgcctgatc actattgctg tacctcgtat
     8521 ccgtgacttc cgtggcctgt ccgctaagtc tttcgacggt cgtggtaact acagcatggg
     8581 tgtccgtgag cagatcatct tcccagaaat cgactacgat aaagtcgacc gcgttcgtgg
     8641 tttggatatt accattacca ctactgcgaa atctgacgaa gaaggccgcg ctctgctggc
     8701 tgcctttgac ttcccgttcc gcaagtaagg tagggttact aaatggctaa gcaatcaatg
     8761 aaagcacgcg aagtaaaacg cgtagcttta gctgataaat acttcgcgaa acgcgctgaa
     8821 ctgaaagcga tcatctctga tgtgaacgct tccgacgaag atcgttggaa cgctgttctc
     8881 aagctgcaga ctctgccgcg tgattccagc ccgtctcgtc agcgtaaccg ctgccgtcaa
     8941 acaggtcgtc cgcatggttt cctgcggaag ttcgggttga gccgtattaa ggtccgtgaa
     9001 gccgctatgc gcggtgaaat cccgggtctg aaaaaggcta gctggtaatt gtcaccaatt
     9061 gaatcacggg agtaaagaca gatgagcatg caagatccga tcgcggatat gctgacccgt
     9121 atccgtaacg gtcaggccgc gaacaaagct gcggtcacca tgccttcctc caagctgaaa
     9181 gtggcaatcg ccaacgtgct gaaggaagaa ggttttattg aagattttaa agttgaaggc
     9241 gacaccaagc ctgaactgga acttactctg aagtatttcc agggcaaagc tgttgtagaa
     9301 agcattcagc gtgtcagccg cccaggtctg cgcatctata aacgtaaaga tgagctgccg
     9361 aaagttatgg cgggtctggg tatcgcagtt gtttctacct ctaaaggtgt tatgactgat
     9421 cgtgcagcgc gccaggctgg tcttggtggc gaaattatct gctacgtagc ctaatcggag
     9481 gaaaaaatgt ctcgtgttgc taaagcaccg gtcgttgttc ctgccggcgt tgacgtaaaa
     9541 atcaacggtc aggttattac gatcaaaggt aaaaacggcg agctgactcg tactctcaac
     9601 gatgctgttg aagttaaaca tgcagataat accctgacct tcggtccgcg tgatggttac
     9661 gcagacggtt gggcacaggc tggtaccgcg cgtgccctgc tgaactcaat ggttatcggt
     9721 gttaccgaag gcttcactaa gaagctgcag ctggttggtg taggttaccg tgcagcggtt
     9781 aaaggcaatg tgattaacct gtctctgggt ttctctcatc ctgttgacca tcagctgcct
     9841 gcgggtatca ctgctgaatg tccgactcag actgaaatcg tgctgaaagg cgctgataag
     9901 caggtgatcg gccaggttgc agcggatctg cgcgcctacc gtcgtcctga gccttacaaa
     9961 ggcaaggggg ttcgttacgc cgacgaagtc gtgcgtacca aagaggctaa gaagaagtaa
    10021 ggtaacacta tggataagaa atctgctcgt atccgtcgtg cgacccgcgc acgccgcaag
    10081 ctccaggagc tgggcgcaac tcgcctggtg gtacatcgta ccccgcgtca catttacgca
    10141 caggtaattg caccgaacgg ttctgaagtt ctggtagctg cttctactgt agaaaaagct
    10201 atcgctgaac aactgaagta caccggtaac aaagacgcgg ctgcagctgt gggtaaagct
    10261 gtcgctgaac gcgctctgga aaaaggcatc aaagatgtat cctttgaccg ttccgggttc
    10321 caatatcatg gtcgtgtcca ggcactggca gatgctgccc gtgaagctgg ccttcagttc
    10381 taaggtagag gtgtaagatg gctcacatcg aaaaacaagc tggcgaactg caggaaaagc
    10441 tgatcgcggt aaaccgcgta tctaaaaccg ttaaaggtgg tcgtattttc tccttcacag
    10501 ctctgactgt agttggcgat ggtaacggtc gcgttggttt tggttacggt aaagcgcgtg
    10561 aagttccagc agcgatccag aaagcgatgg aaaaagcccg tcgcaatatg attaacgtcg
    10621 cgctgaataa cggcactcta caacaccctg ttaaaggtgt tcacacgggt tctcgcgtat
    10681 tcatgcagcc ggcttccgaa ggtaccggta tcatcgccgg tggtgcaatg cgcgccgttc
    10741 tggaagtcgc tggggttcat aacgttctgg ctaaagcata tggttccacc aacccgatca
    10801 acgtggttcg tgcaactatt gatggcctgg aaaatatgaa ttctccagaa atggtcgctg
    10861 ccaagcgtgg taaatccgtt gaagaaattc tggggaaata aaccatggca aagactatta
    10921 aaattactca aacccgcagt gcaatcggtc gtctgccgaa acacaaggca acgctgcttg
    10981 gcttgggtct gcgtcgtatt ggtcacaccg tagagcgcga ggatactcct gctattcgcg
    11041 gtatgatcaa cgcggtttcc ttcatggtta aagttgagga gtaagagatg cgtttaaata
    11101 ctctgtctcc ggccgaaggc tccaaaaagg cgggtaaacg cctgggtcgt ggtatcggtt
    11161 ctggcctcgg taaaaccggt ggtcgtggtc acaaaggtca gaagtctcgt tctggcggtg
    11221 gcgtacgtcg cggtttcgag ggtggtcaga tgcctctgta ccgtcgtctg ccgaaattcg
    11281 gcttcacttc tcgtaaagca gcgattacag ccgaagttcg tctatctgac ttggctaaag
    11341 tagaaggcgg tgttgtagac ctgaacacgc tgaaagcggc taacattatc ggtatccaga
    11401 tcgagttcgc gaaagtgatc ctggctggcg aagtaacgac tccggtaact gttcgtggcc
    11461 tgcgtgttac taaaggcgct cgtgctgcta tcgaagctgc tggcggtaaa atcgaggaat
    11521 aagtagcaga tggctaaaca accgggatta gattttcaaa gtgccaaagg tggcttaggc
    11581 gagctgaaac gcagactgct gtttgttatc ggtgcgctga ttgtgttccg tattggctct
    11641 tttattccga tccctggtat tgatgccgct gtacttgcca aactgcttga gcaacagcga
    11701 ggcaccatca ttgagatgtt taacatgttc tctggtggtg ctctcagccg tgcttctatc
    11761 tttgctctgg ggatcatgcc gtatatttcg gcgtcgatca ttatccagct gctgacggtg
    11821 gttcacccaa cgttggcaga aattaagaaa gaaggggagt ctggtcgtcg taagatcagc
    11881 cagtacaccc gttacggtac tctggtgctg gcaatattcc agtcgatcgg tattgctacc
    11941 ggtctgccga atatgcctgg tatgcaaggc ctggtgatta acccgggctt tgcattctac
    12001 ttcaccgctg ttgtaagtct ggtcacagga acgatgttcc tgatgtggtt gggcgaacag
    12061 atcactgaac gaggtatcgg caacggtatt tcaatcatta tcttcgccgg tattgtcgcg
    12121 ggactcccgc cagccattgc ccatactatc gagcaagcgc gtcaaggcga cctgcacttc
    12181 ctcgtgttgc tgttggttgc agtattagta tttgcagtga cgttctttgt tgtgtttgtt
    12241 gagcgtggtc aacgccgcat tgtggtaaac tacgcgaaac gtcagcaagg tcgtcgtgtc
    12301 tatgctgcac agagcacaca tttaccgctg aaagtgaata tggcgggggt aatcccggca
    12361 atcttcgctt ccagtattat tctgttcccg gcgaccatcg cgtcatggtt cgggggcggt
    12421 actggttgga actggctgac aacaatttcg ctgtatttgc agcctgggca accgctttat
    12481 gtgttactct atgcgtctgc aatcatcttc ttctgtttct tctacacggc gttggttttc
    12541 aacccgcgtg aaacagcaga taacctgaag aagtccggtg catttgtacc aggaattcgt
    12601 ccgggagagc aaacggcgaa gtatatcgat aaagtaatga cccgcctgac cttggttggt
    12661 gcgctgtata ttacctttat ctgcctgatc ccggagttca tgcgtgatgc aatgaaagta
    12721 ccgttctact tcggtgggac ctcactgctt atcgttgttg tcgtgattat ggactttatg
    12781 gctcaagtgc aaactctgat gatgtccagt cagtatgagt ctgcattgaa gaaggcgaac
    12841 ctgaaaggct acggccgata attggtcgcc cgagaagtta cggagagtaa aaatgaaagt
    12901 tcgtgcttcc gtcaagaaat tatgccgtaa ctgcaaaatc gttaagcgtg atggtgtcat
    12961 ccgtgtgatt tgcagtgccg agccgaagca taaacagcgc caaggctgat tttttcgcat
    13021 atttttcttg caaagttggg ttgagctggc tagattagcc agccaatctt ttgtatgtct
    13081 gtacgtttcc atttgagtat cctgaaaacg ggcttttcag catggaacgt acatattaaa
    13141 tagtaggagt gcatagtggc ccgtatagca ggcattaaca ttcctgatca taagcatgcc
    13201 gtaatcgcat taacttcgat ttatggcgtc ggcaagaccc gttctaaagc catcctggct
    13261 gcagcgggta tcgctgaaga tgttaagatc agtgagctgt ctgaaggaca aatcgacacg
    13321 ctgcgtgacg aagttgccaa atttgtcgtt gaaggtgatc tgcgccgtga aatcagcatg
    13381 agcatcaagc gcctgatgga tcttggttgc tatcgcggtt tgcgtcatcg tcgtggtctc
    13441 ccggttcgcg gtcagcgtac caagaccaac gcacgtaccc gtaagggtcc gcgcaaaccg
    13501 atcaagaaat aatcggggtg attgaataat ggcaaaggca ccaattcgtg cacgtaaacg
    13561 tgtaagaaaa caagtctctg acggcgtggc tcatatccat gcttctttca acaacaccat
    13621 cgtgactatc actgatcgtc agggtaacgc gttgggttgg gcaacagccg gtggttccgg
    13681 tttccgtggt tctcgcaaat ccactccgtt tgcagctcag gttgcagcag agcgttgcgc
    13741 tgacgccgtg aaagaatacg gcatcaagaa tctggaagtt atggttaaag gtccgggtcc
    13801 aggccgcgaa tctactattc gtgctctgaa cgccgcaggt ttccgcatca ctaacattac
    13861 tgatgtgact ccgatccctc ataacggttg tcgtccgccg aaaaaacgtc gcgtataacg
    13921 cctcgttttc caggtttgtt ggagaaagaa aatggcaaga tatttgggtc ctaagctcaa
    13981 gctgagccgt cgtgagggca ccgacttatt ccttaagtct ggcgttcgcg cgatcgatac
    14041 caagtgtaaa attgaacaag ctcctggcca gcacggtgcg cgtaaaccgc gtctgtctga
    14101 ctatggtgtg cagttgcgtg aaaagcaaaa agttcgccgt atctatggtg tgctggagcg
    14161 tcagttccgt aactactaca aagaagcagc acgtctgaaa ggcaacaccg gtgaaaacct
    14221 gttggctctg ctggaaggtc gtctggacaa cgttgtatac cgtatgggct tcggtgccac
    14281 tcgtgcagaa gcacgtcagc tggttagcca taaagcaatt atggtaaacg gtcgtgttgt
    14341 taacatcgct tcttatcagg ttagtccgaa tgacgttgta agcattcgtg agaaagcgaa
    14401 gaagcagtct cgcgtgaaag ccgctctgga gctggctgag cagcgtgaaa agccaacttg
    14461 gctggaagtt gatgctggca agatggaagg tacgtttaag cgtaagccgg agcgttctga
    14521 tctgtctgcg gacattaacg aacacctgat cgtcgagctt tactccaagt aaagcttagt
    14581 accaaagaga ggacacaatg cagggttctg tgacagagtt tctaaaaccg cgcctggttg
    14641 atatcgagca agtgagttcg acgcacgcca aggtgaccct tgagccttta gagcgtggct
    14701 ttggccatac tctgggtaac gcactgcgcc gtattctgct ctcatcgatg ccgggttgcg
    14761 cggtgaccga ggttgagatt gatggtgtac tacatgagta cagcaccaaa gaaggcgttc
    14821 aggaagatat cctggaaatc ctgctcaacc tgaaagggct ggcggtgaga gttcagggca
    14881 aagatgaagt tattcttacc ttgaataaat ctggcattgg ccctgtgact gcagccgata
    14941 tcacccacga cggtgatgtt gaaatcgtca agccgcagca cgtgatctgc cacctgaccg
    15001 atgagaacgc gtctattagc atgcgtatca aagttcagcg cggtcgtggt tatgtgccgg
    15061 cttctacccg aattcattcg gaagaagatg agcgcccaat cggccgtctg ctggtcgacg
    15121 catgctacag ccctgtggag cgtattgcct acaatgttga agcagcgcgt gtagaacagc
    15181 gtaccgacct ggacaagctg gtcatcgaaa tggaaaccaa cggcacaatc gatcctgaag
    15241 aggcgattcg tcgtgcggca accattctgg ctgaacaact ggaagctttc gttgacttac
    15301 gtgatgtacg tcagcctgaa gtgaaagaag agaaaccaga gttcgatccg atcctgctgc
    15361 gccctgttga cgatctggaa ttgactgtcc gctctgctaa ctgccttaaa gcagaagcta
    15421 tccactatat cggtgatctg gtacagcgta ccgaggttga gctccttaaa acgcctaacc
    15481 ttggtaaaaa atctcttact gagattaaag acgtgctggc ttcccgtgga ctgtctctgg
    15541 gcatgcgctt ggaaaactgg ccaccggcaa gcatcgctga cgagtaaccg gatcacaggt
    15601 taaggtttta ctgagaagga taaggtcatg cgccatcgta agagtggtcg tcaactgaac
    15661 cgcaacagca gccatcgcca ggctatgttc cgcaatatgg caggttcact ggttcgtcat
    15721 gaaatcatca agacgactct gcctaaagcg aaagagctgc gccgcgtagt tgagccgctg
    15781 attactcttg ccaagactga tagcgttgct aatcgtcgtc tggcattcgc ccgtactcgt
    15841 gataacgaga tcgtggcaaa actgtttaac gaactgggcc cgcgtttcgc gagccgtgcc
    15901 ggtggttaca ctcgtattct gaagtgtggc ttccgtgcag gcgacaacgc gccgatggct
    15961 tacatcgagc tggttgatcg ttcagagaaa gcagaagctg ctgcagagta atctgaagca
    16021 atgtaaaaaa acccgccccg gcgggttttt ttatacccgt agtatcccca cttatctaca
    16081 atagctgtac tctttt
//
LOCUS       DAZSIO010000086        15840 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZSIO010000086 DAZSIO010000000
VERSION     DAZSIO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.086"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..756
                     /locus_tag="ACHX5T_002515"
     CDS             <1..756
                     /locus_tag="ACHX5T_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HFR8467675.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFY
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
     gene            complement(825..1781)
                     /gene="yjiA"
                     /locus_tag="ACHX5T_002516"
     CDS             complement(825..1781)
                     /gene="yjiA"
                     /locus_tag="ACHX5T_002516"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR8467676.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(1792..1995)
                     /locus_tag="ACHX5T_002517"
     CDS             complement(1792..1995)
                     /locus_tag="ACHX5T_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR8467677.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            complement(2045..4195)
                     /gene="btsT"
                     /locus_tag="ACHX5T_002518"
     CDS             complement(2045..4195)
                     /gene="btsT"
                     /locus_tag="ACHX5T_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR8467678.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            complement(4588..5100)
                     /locus_tag="ACHX5T_002519"
     CDS             complement(4588..5100)
                     /locus_tag="ACHX5T_002519"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR8467679.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            complement(5118..6676)
                     /gene="hpaB"
                     /locus_tag="ACHX5T_002520"
                     /pseudo
     CDS             complement(5118..6676)
                     /gene="hpaB"
                     /locus_tag="ACHX5T_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            complement(6903..7840)
                     /locus_tag="ACHX5T_002521"
                     /pseudo
     CDS             complement(6903..7840)
                     /locus_tag="ACHX5T_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            complement(7867..8757)
                     /gene="hpaA"
                     /locus_tag="ACHX5T_002522"
     CDS             complement(7867..8757)
                     /gene="hpaA"
                     /locus_tag="ACHX5T_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR8467680.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            complement(8767..10122)
                     /gene="hpaX"
                     /locus_tag="ACHX5T_002523"
     CDS             complement(8767..10122)
                     /gene="hpaX"
                     /locus_tag="ACHX5T_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR8467681.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCPTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            complement(10144..10947)
                     /gene="hpaI"
                     /locus_tag="ACHX5T_002524"
     CDS             complement(10144..10947)
                     /gene="hpaI"
                     /locus_tag="ACHX5T_002524"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR8467682.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            complement(10958..11761)
                     /gene="hpaH"
                     /locus_tag="ACHX5T_002525"
     CDS             complement(10958..11761)
                     /gene="hpaH"
                     /locus_tag="ACHX5T_002525"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR8467683.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            complement(11829..12209)
                     /locus_tag="ACHX5T_002526"
     CDS             complement(11829..12209)
                     /locus_tag="ACHX5T_002526"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR8467684.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            complement(12219..13070)
                     /gene="hpaD"
                     /locus_tag="ACHX5T_002527"
     CDS             complement(12219..13070)
                     /gene="hpaD"
                     /locus_tag="ACHX5T_002527"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR8467685.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            complement(13072..14538)
                     /gene="hpaE"
                     /locus_tag="ACHX5T_002528"
     CDS             complement(13072..14538)
                     /gene="hpaE"
                     /locus_tag="ACHX5T_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR8467686.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            complement(14535..15824)
                     /gene="hpaG"
                     /locus_tag="ACHX5T_002529"
     CDS             complement(14535..15824)
                     /gene="hpaG"
                     /locus_tag="ACHX5T_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR8467687.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
BASE COUNT         3726 a         4139 c         4154 g         3821 t
ORIGIN      
        1 tatactaaga ttttagagaa tgattttgaa cacccttcaa tacgacgaaa aatagcatcg
       61 ctaagtaatt taggggtaga tgagtggatt cctccggtta tggcattcat gaatcgaatg
      121 gctcgtacag aagattttaa tttagatgat ttttctcaat ttattaccgc ctttgagaag
      181 gtatatatgc atgggtggtt aaaaaaacaa attaaaagcc aaagagaaat ggtttgttat
      241 tctgctctgg tagcaataaa taatgatatg ccttttgatt ctgtgataaa tcagataaac
      301 caacatgcag ataattcagg ttttatagct gctctggatg aagatttata tgagcctcgt
      361 ccgaatcaag tgaacttaat aaaagcaatt ttgcttcgct tagatatgga gcaacaagat
      421 gagagcgtga ttaaaaccta tacggggaga atcacaattg agcatattct tcctcaagca
      481 ttagttaatg aatactggat taatagattc caacctcaag agcatgtcta ctggctgcat
      541 aaaataggta atttaacact cataagtggt tccaaaaatt ctgaaacaca acactatgat
      601 tttatcaaaa agaaatctat ttatgagaaa cttaatagta aaagctcttt ttatttaaca
      661 aaagatgttt gtaattcttc agaatgggga ttggctgaac tgaaaatgag acatgaaaaa
      721 atgaaaacgc aattgaagaa gctttggttg gtctgacata tctaaagagt tccactcgta
      781 ataatgttgc ggtggaactc tcaaaaagtt gatctttccc gaacttactt cctcaatccc
      841 gcaaacgccg cccgaatctc ttcctccggc aactgaatcc cgataaacac cattgtgcta
      901 tgcggttttt catcgcccca cggcctgtcc cagtcggcgc tgtagaggcg ctggacgccc
      961 tggaacagca ggcggttagg ttcgccgtca atccacagca tccctttgta acgcagcagt
     1021 ttatccgccg attccagcag caggttttcc atcacgcggg aaacttcgct gatatctacc
     1081 gggtaatcca gttccaccac aattgacgaa atatcgtttt gtttatccgc gataaagtgg
     1141 aaacgcggtt tggtgctgac gacgttctct tccagcataa aaccgttggt gttgaacagc
     1201 aggcccaggt cgatgtcgcc gtgggtgacg gtgtagaccg gtgcgcgggc gttgatgcgc
     1261 gccaggcgtt cacgcagttt ctctgcttcg cctgcgacgt cggttttagt cagcagaata
     1321 cggtcggcgt agcccacctg ggactgggcg atggtgaact ggttcatctg ctcatcggca
     1381 tgtaccgcat ccaccagcgc aatcacgccg tccagcaggt agagctggca taaaatctca
     1441 tgggagaaaa aggtctgaat aatcgggccg ggatcggcca tgccggtgca ttcaatcacc
     1501 agacgatcga actgaatatt gcccttgtcg agattatcca gcaggtcgag tagtgcgtcc
     1561 tccagctcgt tggagcgcga acagcagatg cagccgttgg tcagcgtttt gatctgcgtg
     1621 gcgcggtcgc caatcagttg gtcatcaacg gagacttcgc cgaattcgtt ttcaatcacg
     1681 gcaatcttgt agccgtgttg ttcgttaaga atatggcgca gcagggtagt tttgcccgcg
     1741 cctaaaaaac cggtgagtag ggtaactgca atcgggttca tgctctctcc attaacaaca
     1801 gcgcataccg cctttaccat cgccgccgta gcgcgcgttc tggcgttcgc ggaagaattc
     1861 ttcatagctc atgtacggct tgtcgggatg gttggtcttc atatgctcga cgtagttgtc
     1921 gtagtctgga ataccaatca acatcttcgc cgcctggccg agatattttt ttgcctgtcc
     1981 taagttacca aacatagttc actctgataa gaacaaagcc ccgccgaagc ggggctaaac
     2041 acggttagtg gtgcgaagag atcttcacgc cgccttccgg gattggaacg tacggtgttt
     2101 ctttgtcagt acgtttgtcg ctgttacgca ccgcaagcca ggttttgaaa ccgtagaaga
     2161 tgatgctgta caccacaatc aggaacagaa tactcaggcc tgcgttggtg tagttgttca
     2221 caacgatgtg gttcatgttg gcaatctgct gcgccgtcag gtcagtaccg ttagcaatct
     2281 tctctttgta ctggcttgcc atgtagaaga agccttccat ctgcgggttg gtgctgaaca
     2341 gtttcagacc cagcgcccag gtggtgcaga taagcagcca tacagccgga acaacagtta
     2401 cccagatgta ttgggtgcgc ttcatcttaa tcagcacaac ggtgcccagt accagcgcta
     2461 cggctgccag catctggttg gagataccga acagcggcca caggctctta acgccgccca
     2521 gcggatcgac cacgccctga tacagcaggt agccccacag acccacacag cccgcagtac
     2581 cgatgatacc ggcaaccaga gagtcggttt ttttcaggaa cgggatgaag ttacccagca
     2641 ggtcttgcag cataaagcgg ccagaacggg tacccgcatc cagcgcggtc aggatgaaca
     2701 gggcttcgaa cagaataccg aagtgatacc agaagcccat gtcagccatc ggcagcactt
     2761 tgtggaacac gtgagcgata cctaccgcca gcgtcggcgc accacctgca cggttcagga
     2821 cagaaggctc accaatgtct ttcgcggttt gcaggatctg ctctggcgaa atcacgaagc
     2881 cccaggagct gacggtcgct gccgcgtgtg cggtaacgtc tttcagctgc gccatgatga
     2941 tcggcgcgtt ctcgccaccc atttcatgca ggttaggcat ggtgatgcca aggccagcag
     3001 gcggggtgtt catcgcgaag taaagacccg gttcgatgat ggacgctgca accagcgcca
     3061 taatcgccac gaaggactcc atcagcattg cgccgtagcc aatgaaacgc gcgtcggttt
     3121 cgttagccag cagtttcggc gtcgtaccgg aagagatcag cgcgtggaag ccagataccg
     3181 caccacaggc gatggtgatg aacaggaacg ggaacagagc gcctttccac agcgggccag
     3241 taccgtcaat gtactgggtc atcgcaggca ttttcagttc cgggttcagc accacgatac
     3301 ccagcgccag gccgacgata acgccgattt tcaggaaggt tgccagatag tcgcgcggtg
     3361 cgaggatcag ccacaccggc agcagtgcgg aaacaaacgc atagccaatc agcgcgaagg
     3421 taatggtggt gtctttaaag gtcagtgccg gaccccagta cggatcgtga gcaatcacgc
     3481 caccgaagta gatagaggca accagcagca cgataccaat aacagagact tcacccacac
     3541 gccccggacg gataaagcgc atgtagatac ccataaacag cgcaatcggt acggttgagc
     3601 aaacggtgaa gacaccccac ggactttcgg ccagggcttt aaccacaatc agcgccagaa
     3661 cggcgaggat gatgatcatg attaagaaac agccaaacag cgcgatagtc cccggtactg
     3721 gccccatctc ttctttgatc atctcaccga gagatgcgcc gttacggcga gaggagataa
     3781 acagcaccat aaagtcctgt accgcaccgg ccagcactac ccccgccagc agccacagcg
     3841 tgccaggcag gtagcccatc tgcgcggcga gaaccggtcc caccagcgga ccagcaccgg
     3901 cgatagcggc gaagtggtga ccaaacaaca cgtaacggtt ggtcggaacg tagttcagac
     3961 cgtcgttgtt aataaccgca ggcgtcgcgc gcgtggggtc gagtttcatc accttctggg
     4021 cgatgtacag actgtagtag cgatacgcca ccagatacac cgacacagag gcgaccacga
     4081 tccacagggc gctgacgtgc tccccccgac gtaatgcaac taccgcgagg cagaatgcac
     4141 cgatgattcc gagaatcacc cagggtatgt gcttgaatag ttttttcgta tccatagtaa
     4201 aacctggcat tttactcgtc gtcttttggc ttacaggtgc ggcagctgcg ttcgttcacc
     4261 tctgacactt acttttgtaa gctcccgggg attcactcac ttgctgcctt cctgcaaacc
     4321 aaaatccata gagtaatttg ttaatcatag gccgaagccg ttctaggttt gcgttgcgtt
     4381 tgaggaggta aattgaccgc tatcctgcag tgatgttgcc agagatacgt gcgcgtaaag
     4441 taaggtaaat aactgagtgg ttattttagg gatgtaagcg gtcaggaatg gcggtgagcg
     4501 gttgggcaag aattattcat cgggtagata tgtgattgag atcacaataa aaatgcccct
     4561 tcgacgaatg cgtgaagggg ctggagctta aatcgcagct tccatttcca gcatcaccgg
     4621 atggaaacgg cgtttaaagt aaataagtcc gtgaccttct gcactgagga tgatgttttt
     4681 aatctccacc agatacacca gatgtgtgcc aatcgcctgc acatcgcgga tctctccttc
     4741 aagactggcc agcgaacctt ttagcaccgg ctgcgccagc ggaccttttt gccagcatga
     4801 gaggctaaaa cgctcttcca tcgccatgcc tgtcatgccc gcgaagtggc gcgccatcag
     4861 ttcctgctca tggttgagga cgttgacgca caacttgccg ttgccctgaa aaaccgggtt
     4921 catcgcactg ttggcgttaa tgcacaccat cagcgacggt ggtgtatccg tgaccgagca
     4981 gacagccgtt gccgtaatcc cgcattgtcc ggcgtcgccc tcggtggtga taatatttac
     5041 cgctgccgac aggctggcca tcgcgtcatg aaagcgcagg cgttgttcat ctaattgcat
     5101 cttaacctcc tgctgcgtta tttcagcagc ttatccagca tgttgatatc gtcgttgttg
     5161 tgcaggtgcg gcacagtcca gccgttctgg tcgtattccg acaggcagcg atcaaccatc
     5221 gccatcatct tgtccatatt gccggagctt tgtgcctggc gcagacattg caggcgaatc
     5281 tcatcctggc taccggagta gttgatttca tacagctcgt gacgaccacc aaattcgctg
     5341 ccaatggcgt cccacatcag tttgaggatc ttgatgcgct ggacgtgatc cataccgttc
     5401 gaaccgcgca catacttcgc cagatactgg tcgatctgcg gattgttcag gtcacgggca
     5461 ctggaaggga ggtagatcag gccactggta acgttgcgtt ctataatgtt tttgatcttc
     5521 gcgtaggcca ttggtgccag tacgcgatag gtttgcagtg cggcatgatc cggtaaataa
     5581 gccccgttga cccacggcgt cgcttctgaa cacatcgagt cactcaatgc ccagaaggtg
     5641 ttgcgccacg ccaccacttc accgagatcg gcctgcacac cacggaactc cagggtgccg
     5701 gtacattcga gtgatttttt cagcagtgcc gtaatgaagt cgagtttcac tgccaggcgc
     5761 acacaggctt gcagcggata catacgggcg aaaccgcctt ccatcgtcca gcgacggcag
     5821 cgatcaaaat cgcggtagat cagcacgttt tcccatggga tcagtacgtt atccatcacc
     5881 agaatcgcat cgttctcatc gaagcggctg gagagcgggt agtcgtatgg cgagccggta
     5941 gcacccgcga ccatctcata agaggcgcgg gagattaatt tcacgccatc ggcatccatt
     6001 ggcgcaacga acatcagtgc gaagtccggg ttttcgccca tcacttgtgc cgagccgaag
     6061 ccaatcatgt tgtagtgagt cagcgccgag ttggtggcaa ccactttcgc accgctgacg
     6121 ataatcccgg cgtcagtctc tttttccagc ttgatgtaaa cgtctttcac tttatcggtc
     6181 ggcaaatgac gatcggtggg ttaacaatcg cgtggttaaa gtagaggcca gtttcctgaa
     6241 tacgggtata ccagttacgg gcgttctgct cgaactgacc gtaaaagccc ggattcgcgc
     6301 ccagtgcgca accgaaagcg gctttgtagt ctggggtacg gcccatccag ccatagctca
     6361 ggcgtgacca ttcagcgatg gcgtcgcgtt gctggcgcag gtcgtcggca cttttcgcca
     6421 cgcggaagaa tttatgggta tagccgccgc tgccggtgtc ggtgttccaa cacagagagt
     6481 cctgcatctc cggtttgtgc agcgcgtcgt acagttgggc aacagacgct gccgcattac
     6541 gaaatgccgg atgagtggtg acgtctttca ctcgctcgcc atagatatag atctcgcgac
     6601 catcctgcag gcttttcaga tactcttccc cggtgaaagg acgttgggta ctggcgcgga
     6661 aatcttctgg tttcatgtcg acctctacag tattaatcgg aatacgattg ttaattttgt
     6721 gtgttgtttt tgttatttaa ttgaagcgtt gaacgcggtt gtcgggaaga gacgtttcag
     6781 gcgaacgttg gtacttttcg acagcaaggt ggagttaaat agtaaactgc gatcgtgatc
     6841 aacttacagt aaggcaaatc ataatattcc ccgctaacaa gggctaacgg ggacggaatt
     6901 ttttatgggg cgaatggaat ggtaatcgtc atattttcat gaccaacgac gataggacct
     6961 gaatagtttt ctgtgcttct tgcttccatg tttcatccgg aacggtgatt ggtaccaggt
     7021 ggctaaggat gagttttttc acttttgctt cattggcaat tttgcctaca tcctcaatag
     7081 tcgtatggtg agaagcaatg gcttttgcca gtgttttacc attgccaata ctgtctgcca
     7141 gcttagcgac ggagggctca tgtacggcct catgcacaag tatatcagcg ccttctgaga
     7201 aagttgccag tgggggaaag tacgaggtat ctcctgagaa gacaattcgc ttgccttgtg
     7261 tgtcgaagcg ataagcaaaa gcctcaccgt cagggaaagg agaatgaggg acctttaatg
     7321 cagtcacggt cacggtatca tctttataaa ctacgccttc accaacatct tgagctgtaa
     7381 cgagtttgcg aggatcgggt tttccttctt cttccatacg cagtgtgatg tcccgatcga
     7441 agacctgcca actggcagcg gtcatggctc gggtgcctgg cggaccgaat acatgaatag
     7501 ttttgtcctg cagaccgcta gcccatgcat tcattaagac tgatggatag tccagaatat
     7561 gatcactgtg taaatgggtg agtaaaatat tatcaatctg gcgcagtgga atattatttt
     7621 ttaccagttg taagctggcc ccgtaaccag catcaattaa ccaggtttta ttgccaataa
     7681 taagtgccgt tgccatcggt aaacggtcag cttttaataa agaagggcca cctttcgttc
     7741 caagtagtac aattttcatt tattgcggtt cggaggcgta ggtgaatgcc actggcgcaa
     7801 tcaggcacgc cacaaatagt gagcgtaaag atattttcat gtcgagtctc tcaggttgat
     7861 aaatggtcag cgaccacgga attgtgttgg tgagcaaccc atcattcggt tgaaaaaacg
     7921 ggcgaaatag gcgggatctt tgtagccaag atcaagggct atctggttga tgctttgctc
     7981 gctatagagc agaagccgtc tggcttcccg gctcactcgt tcgaaaatca atctcttcgg
     8041 cgattgattg gcaaaacgtc ggcacagctc ggtaagccgt gattcactca taccgagcat
     8101 tgttgcatat tcagggacca ttaaatgttg gcgataattt tcgtcaatca gccgattaaa
     8161 cttctggaat aaacacatct cgccgcgcac gctgcatgtt gaaatatcat ttggtggtgc
     8221 ttcacgcagt aggattgtaa aaatactttg cgccagtgat tgcaatagaa tttcccgtcc
     8281 ttgcttattt tgttgaaact ccagggatat tatcggccaa taatgattta atgccgccag
     8341 tgtatgttcc attccctcca gtgacagaca gataccttgt agcattgcgg catctccact
     8401 accaggccag agcttttcta ccaggggcca aattaactcc tgacgtacgg tcagaacatg
     8461 cccatcgcta tcaggatcag taaagaaagt atgtggcacc gatggtggtg ttagtacaaa
     8521 aagcggtgcg cgcacagaat agaagtgctc atccagttgt aaagtaatgc gtccagtgac
     8581 cagataatgg agttgaaaat atctatcgtg ccagtgaggg cgcatatcgc gtccaaagaa
     8641 attcgctaac cgggcaaatg tttcataatg cacatcttca ccagcgtagc gggcatcata
     8701 ttcccggacc aaatcaatat tttcataacc atgttcaaag atagcttcac tggacatcat
     8761 gactccttat tgcggttttg gtgcgataag ttcagatgaa agagagtgtt cccgactccc
     8821 catagggatt cgggtcatga ctaacgcgcc cagtaccagc aatcctgcta caaaccacaa
     8881 acctgagcta aaactgccgg ttgcatcttt caaaataccg ataagtaatg gactgactgc
     8941 tgaacctaca ttgccgacag cattgatgac agccatcgca acggcacgcg attgcaagct
     9001 tataacttga tccggtgttg tccaaaagat agtcatcgcg gtaaatgatc ccattgaggc
     9061 cataatgatg ctgctcagtt gaatgaggct gtgatctgtc gcagaagcga gtaaccatcc
     9121 ggcagcggca aacaggtagg gaagaatcgt atgctttttg cgttctttaa gacggtcgga
     9181 acgacgactc caccagatca tgccaaaaat tgtgcagaat tgggggatgg ccgccagcaa
     9241 accaatcatg atgttgctac tgccagtatt gaaactctgt aggatttgcg gcgtccagat
     9301 attaatcgca ctcagagtat tagtcgggca aaaataagca agtgtatata gcatgatggc
     9361 tggggtcagg acttcacgta aggttgagcg tggaacagat gcatgggcaa atgcactctc
     9421 ttgttcacgg agaatcatcg tttttaaagc ctgtttttcg ttttcatcaa gccaggtcgc
     9481 ctggtctggt gtgtcgttga gataaaacca ggtgacgaca ccaagaatga ccgatggcag
     9541 accttccagc aaaaagagcc attgccagcc ttttagattc cacagtccat ccaaggccag
     9601 aatgtagcct gaaaggatca atcccagcat catagtgact ggcatcgcta tcataaataa
     9661 ggcattggct ctggcccgat gatatgccgg gaaccaccag gtcagataaa ccaaaatacc
     9721 aggtaaaaaa cccgcctcag caataccaac cagcatacgc agtagataga gtgtttcggg
     9781 gcttgttgca aacattgtgc aggtggaagc aattccccag accaccatga tcccagcgat
     9841 ccatcgacga gcgccaattt tcgctaacat catattactg gggatcccgc acatgacgta
     9901 cgtgacataa aagagtgttg cggcgaggcc aaacattgtg gacgtcaggc caagatcctt
     9961 gcccattgtc agtccggcaa atcctatatt gatgcgatca agaaacgaaa aaacaaacag
    10021 aataaaaaga aaaatgatta ggcgacgaaa taatttatta atgacacgat gttcaatttc
    10081 aacagtttta ttatctttat attgcagggt agaggtcgtc atgatgcgct ccacatcgta
    10141 cgtttagtag acagagttgt tgttttgctt cgccatcgaa atagaagaac tattaccaaa
    10201 ccgtgctgcc agcgcttcgg cggcgcgggc gagcagggtg gtgtcaacgc cgacggcgac
    10261 aaacagcgcg cccagttcca gatagcgttt tgccagttgc tcattggcga tcaggatccc
    10321 cggcgctttg cccgcttcgc ggatctgcac gatcgcctgc tcaatggcgg cctgtacttc
    10381 cgggtgctgc ggattaccgg cataacccat atcggcgctc agatccgccg ggccgataaa
    10441 cacgccgtcg accccttcca cgtccagaat ctgcggtaag ttcttcattg cctcacgcgt
    10501 ttcgatctgc accagcacgc acatttgatc gttggctttt tgcaggtaat caggaatgcg
    10561 attccagcgc gaggcgcgag ccagcgcact gcccacaccg cgaataccgg cggggggata
    10621 acgggtggcg cgtaccgctt tacgggcttc gtcggcgttt tgtaccatcg gcaccagtaa
    10681 ggtttgtgtg cctacgtcca gcagttgttt gatttgcacc ggatcgttcc acgatgggcg
    10741 taccactggc tggctggggt agggcgcaat cgcctgtagt tgggtgagca cggtttgcac
    10801 gttattcggc gcgtgctcac cgtcgatcaa taaccagtcg aatcctggtc cggccagtaa
    10861 ctccgcgctg tagctgctgc tcagccccag ccataatcca atctgcggac ggcctgcttt
    10921 cagcgccgct ttaaaactgt tttccatcgt tctctcctta aacaaagcgg cagctaatgg
    10981 agcccatgtt gccgtaatcg acgtggaagg tgtcgccctt acgcgccgga accgggaggg
    11041 tgaacgaacc accgagaatg atttgcccgg tttccagttg tacgtcatac ggtgcgagtt
    11101 tgttcgccag ccaggccacg ccgtttgccg gatgattcag cacaccagcg gcgacgccgg
    11161 tttcttcaat cacgccattg cgatacatca gggcggagat ccaacgcaga tccagttcgt
    11221 ctggtttgat cggacgacct ccgaggatca ctccggcgtt ggcggcgtta tcggaaatgg
    11281 tgtcgaatac tttgcgcggg cgctgggttt ccggatcgat gttgtggcag cgagcgtcga
    11341 tcagctccag cgccgggatc acatagtccg tggcgttgta aacgtcgaac agcgtgcagt
    11401 ttggcccacg cagcggtttt gccagcacaa aagccagctc cacttcaatg cgcggcacga
    11461 taaagcgatc ggtcgggata tcgctgccat cgtggaagaa catgtcgtcc agcagtgcac
    11521 cgtaatccgg ttcgctgatc tgcgagcttg cctgcatcgc tttcgaggtc agaccgattt
    11581 tgtggccttt cagcgtgcga ctttcggcga ttttcaggcg aacccattcg cgctgcacgg
    11641 cgtaggcgtc ttcgatggtg atctccgggt aatccagcga gatcgcgcgg atctgttcgc
    11701 gctgtttttc tgcctgatcc agacgctggg cgatcagggt gtgggtgtgt ttatcgaaca
    11761 tagcgatatc ctgtggcatt gccggatgcg gcagcgcctt atcatccggc ctggaagctg
    11821 gtgcaaattt atttaaacaa cgcgtgtacg ttgttctgtt tgaaattcaa cgtcgggtgc
    11881 agctctgcaa tctcaaatga aagcgctagg taacggctct ccatcagtga ggcgaagtgc
    11941 gttttaatca gatcaaacag catttcgccc acttcctgac ggctttccag actgcgtccg
    12001 ctgccgattt ttagcgtcat atgcacaaag gcgtaatcgt gcttgccatc agccatttgc
    12061 caggtatcga tccagtgcgc ccggctacgt attcccccaa gcggaaaaat ccccgttgcc
    12121 gccagtgcag tgttaacact ggcaaacagc tcaggtaagc gggcttcttc acgaatattt
    12181 tcagtacatt ccacgataaa gtgcggcata aaacgtcctt acgcgggcag cgggaaaaca
    12241 gcgttaacct gaccggtgcc agagcttggg aacagctcgg taataaactc caccttgccg
    12301 tcgtatttat cccagccgag catccccagc agcatcaccg tgtcgtgcat attgccttcg
    12361 ccgtagcagt agtcggcgta ctccggcagc atattgcaaa actctttgaa ctggccttcg
    12421 cgccacagct tcaccacacg ctcgtccatc tggcggtcga actcgcgggt gtagctgttc
    12481 atcccttctt ccgcacgctg atcgtcaata aagcggtgcg ataacgaacc gctggcaagg
    12541 accgccacgg tgccgtcgta ctgttcgatc gctttcagaa tcgcttcgcc cagcttgcgg
    12601 ctgtcggcaa aatcgtgaac cgtgcagaaa gccgaaatgg agaccacttt gaagtgcttg
    12661 tcttcattca tgtagcgcat cggtaccagc gtgccgtatt ccagtttcag gctgggaata
    12721 ttgtgcgctt ttgcccgcac gccgagcttt aaggcttcct cggcaattag ctgccccaac
    12781 tccgggttgc cctcgtagtt gtaggtcatg tcgcgaataa aatgcggcag ctcgttgctg
    12841 gtgtagacgc cttcaaaatg gtctgcacag ttgatgtgat aagcactgtt gaccagccag
    12901 tgagtatcga aaacgataat ggtatcgacg cccatttccc ggcaacgctt gctgatctct
    12961 ttatgcccgt cgatcgcacc ctggcgacaa ccgtggtttt ttcctggtag ttcagagaga
    13021 tacatcgacg gtacgtgcgt gatcttggcg gctaacgcta acttacccat atcagactcc
    13081 ccatttcgga attggatggt cgcccatgga aatgcagacg ttcttcattt ccgcgaacac
    13141 ttcgaagctg tactcaccgc cttcacgccc ggtgccggag gcttttacgc cgccaaatgg
    13201 ctggcgcagg tcgcgcacgt tctgggtgtt gacgaacacc atgcctgctt caatgccacg
    13261 cgccaggcgt aacactttgc tgacatcctg tgtccagatg tacgacgcga ggccgtactc
    13321 cacgtcgttt gccaggcgta agccttcggc ttcgtcttta aacggcagca ggcaggcgac
    13381 cggcccgaaa atctcttcct gggcaacgcg catacggtta tctacgtccg ccagcaccgt
    13441 tgggcgcagg aagttgccgc ctttcaggtg cgcaggcagg tcggacggtt tatccgggcc
    13501 gcccgccaac agggttgcgc cttcttcaat gccgagacgg atatagccgg agactttttc
    13561 ccagtgttgc tgactgataa gcgccctaac ctgggtattc ggatcgttcg gatcgcccac
    13621 gcgcagacgg ttggcgcgtt cggcaaagcg tttaacgaat tctggataga tgctctgctg
    13681 aataaagatg cgcgaaccgg cggtgcagcg ttcgccgttg attgagaaga tggtgaacag
    13741 ggcggcgtcc agcgcacgct caatatcggc atcttcaaaa atcagcaccg gtgatttacc
    13801 gcccagctcc atggagtatt ttttcagccc ggcgtttttc atgatgttgc gcccggtggc
    13861 ggtaccgccg gtgaacgaca cggcacgcac gtcatgatga cggaccagcg catcgcctgc
    13921 ggttgcgccg tagccctgta ccacgtttag cacgcccgcc ggaataccag cttccagcgc
    13981 cagctcaccc aggcggtcgg cggtcagcgg ggagagttcc gacatcttca gcaccgcggt
    14041 attgcccagc gccagacacg gcgcgacctt ccaggtggcg gtcataaacg gcacgttcca
    14101 cggcgacgcc agcgcacaaa cgcctaccgg ttgcaccagc gtgtagttga gcatcttgtc
    14161 gtcaaccgga taggtcttgc cgttcatctg ctggcagact tccgcgaaaa attcaaagtt
    14221 gtgagaagcg cgtgggatca acacattttt ggtctgatgg atcggcaggc ccgtgtccgc
    14281 ggtttccatc gcggcgatct caggcacgtt ctggtcgatc agatcgccca gacggcgcat
    14341 caggcgcgca cgctctttca tcggcagatc agcccatttc gggaacgcct ctttcgctgc
    14401 cgctaccgcc tgattgatct ccgcttcacc gccagaggcc acatccgcca gcacttcacc
    14461 cgttgccgga ttggtagtct ggaagtagtc gttacctgca acatttttgc cgttgatcca
    14521 atgatttact tttttcattt cgctgtttcc tcactcacaa ttcggttcac caggcggccc
    14581 acgccttcta cttccaccac tacttcatcg ccaggcacca cgtcagataa gccttttggt
    14641 gtgccggtgg cgatcatgtc gcccggattc agggtcataa attcgcttaa gtaggcgatc
    14701 aggaagggca cgctgaagat cagatcggcg gtggtgcctt gctggcgtaa ctcgccgttg
    14761 acgaaggtgc gaagggtcag attatgcggg tccgggatcg cctctttcgg cacgatggtt
    14821 gaaagcatcg gcgtcagtcc gtcgcggctt ttgacccgca ggttagggcg gtagtagttt
    14881 tccagatagt cgcgaatggc gtagtcgtta cacacggtgt agcccgcgac ataatccatg
    14941 gcatcggctt cgctgacgtt acgcgcctgc ttgccaataa ctaccaccag ctccgcttcg
    15001 tagtgcatgt attcaatatt gttcgggcgc acggaggtct ggttgtcgcc agtgagggta
    15061 ttcggcgctt tcaggaacac cagcggctct tccggtggct taaattccag ttcgctggcg
    15121 tggtcggcgt agttcaggcc gagggcaaac agcgtaccgt gcgggtgtgg cagcgttggg
    15181 aagctcttgc gcgtggtcac ttcacgttcg tccactaccg gattttccag cggcgggaaa
    15241 ccttctgcga gaacacgcac gcgatcgctt ggctgtattt ccacgcgcgc ctgtggcgtg
    15301 ccgagcagaa tagcatcgcc tggattcagc gtggcaaatt cgctcagggc gctcagcagc
    15361 tgtgcggcgt tacgttgtaa atcggcggta ttccagtgat cggcaggacg cccgttgatc
    15421 tcggtataga tagtcagatt atcgacattg ctgagagcca cggtttcgcc aatggggcag
    15481 aatccatcac gacattttgc tttgattgcc ggacggtaaa agctctcttc cggcaggctg
    15541 acgtcgttat ccagcgcata tccggcgatg tactctgccg catcttcttc acgtactttc
    15601 gtcgccgttt tccccacaat cagcgcaaca gtcgcaccgc tcagtacttt ttcaccctgt
    15661 ggaaagggaa tcggttcacc gcaaccaatc accgtattgc gcggtttaat aaaccagacc
    15721 gcagttttag gcggggcttt gtagggagat tgctggaacg cttcctgcca ggcatcaagt
    15781 tggctgcgat ggttcaacgc tacggcgaag atagtgcctt tcattaccac tcctcggatt
//
LOCUS       DAZSIO010000087        15768 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZSIO010000087 DAZSIO010000000
VERSION     DAZSIO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15768
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.087"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACHX5T_002530"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACHX5T_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
     gene            complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACHX5T_002531"
     CDS             complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACHX5T_002531"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR8467688.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACHX5T_002532"
     CDS             complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACHX5T_002532"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR8467689.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACHX5T_002533"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACHX5T_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="ACHX5T_002534"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="ACHX5T_002534"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR8467690.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="ACHX5T_002535"
     CDS             complement(5877..6218)
                     /locus_tag="ACHX5T_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR8467691.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACHX5T_002536"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACHX5T_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR8467692.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACHX5T_002537"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACHX5T_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR8467693.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12574
                     /gene="msrA"
                     /locus_tag="ACHX5T_002538"
     CDS             11936..12574
                     /gene="msrA"
                     /locus_tag="ACHX5T_002538"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR8467694.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12897..14240
                     /locus_tag="ACHX5T_002539"
     CDS             12897..14240
                     /locus_tag="ACHX5T_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR8467695.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14302..14508)
                     /locus_tag="ACHX5T_002540"
     CDS             complement(14302..14508)
                     /locus_tag="ACHX5T_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR8467696.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14833..15387
                     /locus_tag="ACHX5T_002541"
     CDS             14833..15387
                     /locus_tag="ACHX5T_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR8467697.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15450..>15768)
                     /gene="ytfI"
                     /locus_tag="ACHX5T_002542"
     CDS             complement(15450..>15768)
                     /gene="ytfI"
                     /locus_tag="ACHX5T_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFR8467698.1"
                     /translation="DNFAIVIESLGFALKIYGGDERFRNGSSVVLEDEDYSLIYNFLV
                     NAGCQQVELAEQVDAIVSANLAADSNITKEKICEKYKSTIEAFKKEQLALPVLVRCKN
                     SET"
BASE COUNT         3738 a         4332 c         3901 g         3797 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aatcgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt
    12601 gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat
    12661 cgcccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca
    12721 gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc
    12781 tatactagcc actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc
    12841 acacaaatta atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt
    12901 taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg
    12961 agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata
    13021 taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc
    13081 aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc
    13141 cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct
    13201 ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga
    13261 aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct
    13321 tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct
    13381 tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg
    13441 cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg
    13501 aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg
    13561 tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc
    13621 cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt
    13681 ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac
    13741 aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt
    13801 ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga
    13861 tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg
    13921 aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg
    13981 acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca
    14041 tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat
    14101 tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg
    14161 tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata
    14221 aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct
    14281 tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt
    14341 aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc
    14401 cttatcaaat tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt
    14461 tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg
    14521 ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta
    14581 atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct
    14641 aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt
    14701 catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg
    14761 ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa
    14821 gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg
    14881 atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat
    14941 cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag
    15001 ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa
    15061 aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc
    15121 accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt
    15181 ctggagagta ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc
    15241 gcgcgcggtg cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac
    15301 ggtcgcgtgc aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg
    15361 gacctgctgc aaaagttgct caaataatgg caataacata aattctggaa aaataatttt
    15421 cccctatcgt tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat
    15481 actggtaacg ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa
    15541 atcttttctt tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact
    15601 tgttcggcta attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag
    15661 taatcttcat cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag
    15721 attttaagcg caaagcccaa agattctatg actatggcaa aattatca
//
LOCUS       DAZSIO010000088        15625 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZSIO010000088 DAZSIO010000000
VERSION     DAZSIO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.088"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            68..586
                     /locus_tag="ACHX5T_002543"
     CDS             68..586
                     /locus_tag="ACHX5T_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR8467699.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            576..1801
                     /gene="dgcN"
                     /locus_tag="ACHX5T_002544"
                     /pseudo
     CDS             576..1801
                     /gene="dgcN"
                     /locus_tag="ACHX5T_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            1817..2299
                     /locus_tag="ACHX5T_002545"
     CDS             1817..2299
                     /locus_tag="ACHX5T_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR8467700.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(2376..2723)
                     /gene="rplS"
                     /locus_tag="ACHX5T_002546"
     CDS             complement(2376..2723)
                     /gene="rplS"
                     /locus_tag="ACHX5T_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR8467701.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2765..3532)
                     /gene="trmD"
                     /locus_tag="ACHX5T_002547"
     CDS             complement(2765..3532)
                     /gene="trmD"
                     /locus_tag="ACHX5T_002547"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR8467702.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3563..4111)
                     /gene="rimM"
                     /locus_tag="ACHX5T_002548"
     CDS             complement(3563..4111)
                     /gene="rimM"
                     /locus_tag="ACHX5T_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR8467703.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4130..4378)
                     /gene="rpsP"
                     /locus_tag="ACHX5T_002549"
     CDS             complement(4130..4378)
                     /gene="rpsP"
                     /locus_tag="ACHX5T_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR8467704.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4515..5876)
                     /gene="ffh"
                     /locus_tag="ACHX5T_002550"
     CDS             complement(4515..5876)
                     /gene="ffh"
                     /locus_tag="ACHX5T_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR8467705.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6043..6834
                     /locus_tag="ACHX5T_002551"
     CDS             6043..6834
                     /locus_tag="ACHX5T_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR8467706.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6855..8141
                     /locus_tag="ACHX5T_002552"
     CDS             6855..8141
                     /locus_tag="ACHX5T_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR8467707.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8196..8789)
                     /gene="grpE"
                     /locus_tag="ACHX5T_002553"
     CDS             complement(8196..8789)
                     /gene="grpE"
                     /locus_tag="ACHX5T_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR8467708.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            8912..9790
                     /gene="nadK"
                     /locus_tag="ACHX5T_002554"
     CDS             8912..9790
                     /gene="nadK"
                     /locus_tag="ACHX5T_002554"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR8467709.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            9876..11537
                     /gene="recN"
                     /locus_tag="ACHX5T_002555"
     CDS             9876..11537
                     /gene="recN"
                     /locus_tag="ACHX5T_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR8467710.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            11686..12027
                     /gene="bamE"
                     /locus_tag="ACHX5T_002556"
     CDS             11686..12027
                     /gene="bamE"
                     /locus_tag="ACHX5T_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR8467711.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12089..12379)
                     /locus_tag="ACHX5T_002557"
     CDS             complement(12089..12379)
                     /locus_tag="ACHX5T_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR8467712.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12369..12806)
                     /gene="ratA"
                     /locus_tag="ACHX5T_002558"
     CDS             complement(12369..12806)
                     /gene="ratA"
                     /locus_tag="ACHX5T_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR8467713.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            12977..13459
                     /gene="smpB"
                     /locus_tag="ACHX5T_002559"
     CDS             12977..13459
                     /gene="smpB"
                     /locus_tag="ACHX5T_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR8467714.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            13674..14036
                     /gene="ssrA"
                     /locus_tag="ACHX5T_002560"
     tmRNA           13674..14036
                     /gene="ssrA"
                     /locus_tag="ACHX5T_002560"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            14241..15482
                     /locus_tag="ACHX5T_002561"
     CDS             14241..15482
                     /locus_tag="ACHX5T_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR8467715.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         3953 a         4110 c         3712 g         3850 t
ORIGIN      
        1 tgatatttta atgagcatcc tgaaaaaata tcgatgcatt tcgagcgaag atggtgggga
       61 tccctgaatg cgtttttctc accgactgtt cttacttctg attctcctgc tgacgggagc
      121 accaatactc gctcaagagc cttctgacgt tgcgaaaaac gtgcgtatga tggtttccgg
      181 tattgtcagt tacacccgct ggcctgcgct atcagggcca ccaaagttat gtatcttttc
      241 atcttcacgt tttagtaccg ccctacaaga gaatgccgcg acatctttgc cttatctgcc
      301 tgtcatcatt cacactcaac aagaggcgat gatttcaggc tgcaatggtt tttattttgg
      361 caacgagtca ccgacattcc agatggaatt aaccgaacaa tatccgtcaa aggcgttatt
      421 attaattgcc gaacaaaata ccgagtgcat tattggtagc gccttttgtc tgatcatcca
      481 caataatgac gtcagatttg ccgtaaacct ggatgcctca tcgcgtagcg gtgtaaaggt
      541 caacccggat gtcttaatgc tcgcacggaa gaaaaatgat ggataacgat aattctctta
      601 ataagcgccc cacgtttaaa agagcattac gcaacatcag tatgaccagc atatttatca
      661 ctatgatgct gatctggttg ctgctttccg tgacctcggt gctgaccctg aaacagtacg
      721 cgcaaaaaaa cctggcactg acagcagcaa caatgactta cagtctggaa gcagctgtcg
      781 tttttgccga tggccctgca gcaactgaaa cactggcagc gctgggccag caagggcatt
      841 ttcaactgca gaagtacgtg ataagcagca aaatattctg gcatcctggc attacccccg
      901 taaggatcca ggcgatactt tcagcaattt cataagccac tggctcttcc ctgcccccat
      961 cattcagccg attcgtcaca atggtgaaac cattggcgaa gtacgcttaa ccgctcgcga
     1021 cagttcaatc agccatttta tctggttttc gctcgccgta ctgaccggtt gtattctgct
     1081 ggcatcaggc atcgcaatta ccctcacccg ccatttgcac aatggcctgg tggaagcact
     1141 gaaaaatatc accgatgtcg tacatgatgt gcgttccaac cgcaattttt cccgacgagt
     1201 ttcggaagaa cgtatcgctg agtttcaccg cttcgctctc gacttcaaca gtctgctgga
     1261 tgaaatggaa gagtggcagc ttcgtttaca ggccaaaaat gcgcagcttc tgcgtaccgc
     1321 gctacatgac ccattaaccg ggctggctaa ccgcgcagcg tttcgtagcg gcatcaatac
     1381 gttgatgaac aattccgatg cccgaaaaac gtcggcgtta ctatttcttg atggcgataa
     1441 tttcaaatat atcaatgata cctggggtca tgcgacgggc gatagggtct tgattgaaat
     1501 cgcaaaacgg ttagctgaat ttggcgggct gcgacataaa gcataccgcc tgggcggcga
     1561 tgaattcgct atggtgctct atgatgtaca gtcggaatct gaagtgcagc agatatgctc
     1621 agcactgaca caaatcttta atctcccgtt tgatcttcat aatggccatc agaccaccat
     1681 gacattaagc attggttacg cgatgaccat tgagcacgcc tctgcggaaa aattacaaga
     1741 gcttgccgat cacaatatgt atcaggccaa acaccagcgt gccgaaaagc tggtgagata
     1801 acaaggatat atcgatatga taaagcacct ggtagcaccc ctgattttca cctcactaat
     1861 actgactggc tgccagtccc ctcagggaaa gtttactcct gagcaagtcg ccgctatgca
     1921 atcttatgga tttactgaat ccgccggcga ctggtcgctg ggcttatcag atgccattct
     1981 gttcgcaaaa aatgactaca aattgctccc ggaaagccag caacagatcc aaaccatggc
     2041 agccaaattg gcctcgacag ggctaacaca tgcccgtatg gatggacaca ccgataacta
     2101 tggtgaagac agttacaacg aaggcttatc attgaaacgg gcgaatgtcg tggccgatgc
     2161 atgggctatt ggtggacaaa ttccacgcag caatctcacc acacagggtt taggaaaaaa
     2221 atatcccata gccagtaaca agaccgccca gggccgcgcc gagaaccgcc gcgtcgcagt
     2281 ggtgattgca accccttaaa acacgagcaa acagacaaaa aaagggccag ccaattggcc
     2341 agcccttctt aacaggatgt cgcttaagcg aaatcttagt taagacgctc tttgatacga
     2401 gcagccttac cagtacgctc acgcaggtag tacagtttag ctttacgaac agcaccacga
     2461 cgtttgacag aaatgctgtc aactaccgga gagtgagtct ggaagacacg ctcaacgcct
     2521 tcgccgttgg aaattttacg aacagtgaat gcagagtgca gaccgcggtt acgaatagcg
     2581 ataaccacgc cctcgaatgc ctgcagacgt tttttggaac cttcaacaac ccatactttc
     2641 acttccacgg tatcacccgg acggaaggaa ggtacgtcct gcttcatctg ctcttgttca
     2701 agttgcttaa taatgttgct cataatttaa tctcttatcc tgggtaaact gatctctcgg
     2761 gggcttacgc catcccatca tgtttatgtt gctgttgtgc gtgttccgtt ttgaactccg
     2821 ccagcaacct tgcttgctct tcagtcagag ccaggttttc cagaagttca ggtcttctaa
     2881 gccaggtacg gcccagcgac tgtttcaaac gccagcgacg tatctcggca tggttgcccg
     2941 acagtaacac tggcggaact tccatccctt ctaacacctc aggccgcgta tagtgcgggc
     3001 aatccagcaa tccttcagca aaggaatctt ccgttgccga ggcttcatgt cccagtactc
     3061 ccggaataaa ccgggaaacg gagtcaatca gcgtcattgc tggtaactca ccaccactga
     3121 gaacgtaatc gccgattgac cattcttcgt caatttcggt ttggatcacg cgctcatcta
     3181 taccttcgta gcgaccgcac accagaatca atttttgatt cgttgccagt tcgctgacgc
     3241 ccgcttgatc aagcttgcgt ccctgtggtg acagataaat cacctttgcg ccttcacccg
     3301 ccgcggcttt tgctgcatga atggcgtccc gcaagggttg caccatcatt aacatccccg
     3361 gtccgccgcc gtaaggacga tcgtccacgg tacggtgccg gtcatgcgtg aagtcgcgag
     3421 gactccaact ctggatgctc agcaggccat ttttaactgc ccggccagtt accccgtaat
     3481 cggtaattgc gcggaacatt tcaggaaaca ggctaattat gccaatccac atagcgccgc
     3541 cttttaccgt ttatccggtg gtttaaaaac caggatccca atctacttcg attgaacgag
     3601 tagtgagatc gactttcttg ataacctgcc catcgaggaa cggtacgaga cgttccttga
     3661 taccaaacgc atctttcagg tttgccttaa tgacgagaac gtcattagat ccggtttcca
     3721 tcatatcaac gactttaccg agatcgtagc cttcagtggt tactacctgg cagcccatca
     3781 ggtctttcca gtagtagtcc ccctcttcaa gctgaggcag ctgcgatgaa tccacgacaa
     3841 tttcacaatt cgtcagcagg ttcgccgcat cacgatcgtc aacgcctttc agcttgatga
     3901 tcatgtcctg attgtggtgc ttccagcttt ccagctggac ttgctgccac tgacccgcct
     3961 tctggataaa ccagggctga tagtcaaaaa tgctttcggc gtcttcggtg gaagaaaaca
     4021 ctctgagcca cccacgaata ccgtaagacg aacccatttt tcccaaaacg atgggatcaa
     4081 caggtgcttg cgcggtgagt tgtttgctca tcatgaccac cgtgacagat taagctgctt
     4141 tgtttacttc tttgatcagc gcagcaacgc gatcagaaat agttgcgccc tggccaaccc
     4201 agtgagcgat gcgatccaga tccaggcgag tgccttcttc tttttcgcta gcgattgggt
     4261 tgaagaaacc aacgcgctcg atgaagcgac cgttgcgtgc attacggctg tcagcgacaa
     4321 caacctggta gaacggacgc tttttagcgc cgtgacgtgc taaacgaata gttaccataa
     4381 catcctcttg tgtgaataaa acaaccggac cccatcgagg aacggagtcc ggtgtcatat
     4441 taaaagcccg aaaattttac tgatttctgg agaaaatgca atcagcagtt aataactctg
     4501 tcgcataagg tcatttagcg accaggaaat cccggcggca tcattccctt catgcttctc
     4561 atcatcttcg ccattccgcc tttcttcatt ttcttcatca tgcgctgcat gtcgtcgaac
     4621 tgcttcagaa ggcggttaac gtcctgtacc tgcatcccgc aaccggcggc gatacgacgc
     4681 ttacgcgaac ctttgatgat ttctggctta gcgcgctctt tcatcgtcat tgagttgatg
     4741 atggcttcca tacgcaccag cactttatcg tccatctgcg acttgacgtt atccgggatc
     4801 tgccccatgc ccggcagttt gcccatcaag ctggccatgc cgcccatatt tttcatctgg
     4861 cgcagttgct caagaaagtc gttgagatcg aagccgtcgc ctttttttag cttgctggct
     4921 aatttctctg cctgcgcgcg gtcaactttg ctttcgatat cttcgatcag cgacagtacg
     4981 tcgcccatgc cgagaatacg cgacgcgatg cggtccggat ggaacggctc cagcgcctca
     5041 gttttctcgc caacaccgag gaacttgatc ggtttgccag tgatgtgacg aatagagagc
     5101 gccgcaccgc cgcgggcatc gccgtccact ttggtcaaca ctacgccggt aagcggtaac
     5161 gcttcattga atgcttttgc cgtattggcc gcatcctgac cggtcatggc gtcaaccaca
     5221 aacagggttt caaccgggtt aatcgacgca tggacttgtt tgatctcgtc catcatcgct
     5281 tcgtcaacgt gcagacgacc agcggtatcc accagcagca cgtcgtagaa tttcagtttg
     5341 gcttctttca gcgccgcgtt aacaatatca accggcttct gaccaacatc agaagggaag
     5401 aaatcaacgc ccacctgctc tgccagcgtc tcaagctgtt tgattgccgc cgggcgatag
     5461 acgtcagcag aaaccaccag cactttcttc ttgtgcttct cgcgcaggaa attaccgagt
     5521 ttaccaacgc tggttgtttt accggcacct tgcaggcctg ccatcagcac gaccgcaggc
     5581 ggttgcgcag ccagattcag ggtctggttc tcttcgccca tcgccgcaac cagttcgtta
     5641 cggacgattt tgacgaactc ctgccccggc gtcaggctct tattaacttc atgaccaacc
     5701 gctttctctt ttacgcgatt gataaactca cgcactaccg gcagagcgac gtccgcctcc
     5761 agcagcgcca tgcgcacttc gcgcagcgta tcttttacgt tgtcttcagt gaggcgtcca
     5821 cggccactga tattgcgcag cgtgcgcgac aaacgatcgg ttaaattatc aaacattgtc
     5881 tctcgcctgg ggtggaaacg gttggccgca atcgcgacac atcatcagta ttttgccgca
     5941 gtataacatg aaggcgtctt tgttgttatg caacggttgg agcagcgttc acctgacgct
     6001 atactgcttc tctttcttat tgctcaaact gtcgacatta ctatgcccgt ttttgctctg
     6061 ctcgcgcttg tcgcctactc cgtcagtctt gcgctgattg ttcccggtct gctgcaaaaa
     6121 aacggcggct ggcggcgcat ggctattatt tctgcggtca ttgcgctggt ctgccacgca
     6181 atcgctctgg aagcccgcat cctgcccgac ggtgatagcg gacaaaacct cagcctgctg
     6241 aacgttggtt cattggtcag tttggtgatc tgtacggtaa tgaccattgt ggcttctcgc
     6301 aatcgtggct ggttgctgct acccattgtc tatgcctttg cgcttatcaa cctggcgctg
     6361 gcgaccttca tgcccaatga atacatcacc catctggaag caacgcctgg gatgctgatg
     6421 cacattggct tatcgctctt ttcctatgcc acgctaatta tcgccgccct gtacgcgctg
     6481 caactggcgt ggattgatta ccaactgaag aacaagaagc tggcatttaa ccaagaaatg
     6541 ccgccattga tgagtatcga gcgtaaaatg ttccacatca cgcagattgg cgtggtgctg
     6601 ctaacgctca cgctttgcac tggcctgttc tacatgcaca acctatttag catggaaaat
     6661 atcgacaagg ctgtgctctc tatcgtggcg tggtttgtct atattgtgct gctgtgggga
     6721 cattaccatg aaggatggcg tggacgccgc gtcgtctggt ttaacgttgc gggcgcggta
     6781 attctgacac tggcctactt cggcagccga attgtccagc agttaatcag ctaaacccag
     6841 aaaaggagtt tcccctggaa cacatttcta ctactacgtt gatcattatt ctgatcatca
     6901 tggtggtcat ttcagcctat ttttccgggt ccgaaaccgg aatgatgacc ctcaaccgct
     6961 atcgtcttcg acatatggcg aaacagggta atcgctcggc caaacgcgtc gaaaaattgc
     7021 tgcgtaagcc agaccgcctg ataagcctgg tgttaatcgg caataacctg gtcaatattc
     7081 ttgcctccgc gctcggcact attgtcggga tgcgtttgta cggcgatgcg ggcgtggcaa
     7141 ttgcgactgg tgtgctgact tttgtcgtac tggtttttgc tgaggtattg ccgaaaacca
     7201 ttgccgcgct gtacccggaa aaagtcgctt atccgagtag ttttctgctg gctccgctgc
     7261 aaattttgat gatgccgctg gtctggttgc tgaatgctat cacccgtatg ctgatgcgca
     7321 tgatgggtat caaaaccgat atcgtggtta gcggctcttt gagcaaagaa gagttgcgca
     7381 ctatcgtgca tgaatcgcgc tcacaaattt cccgtcgcaa tcaggatatg ctgctgtcgg
     7441 tgctcgatct ggaaaaaatg accgttgatg acatcatggt accgcgcagt gaaattatcg
     7501 gtattgatat caacgatgac tggaaatcta ttctgcgcca actctcccac tcacctcacg
     7561 ggcgcatcgt gctttaccgt gattcgctgg acgacgccat cagtatgctg cgagtacgtg
     7621 aagcctggcg gttgatgtcg gagaaaaaag agttcaccaa agaaaccatg ctgcgcgccg
     7681 cggacgagat ctattttgtg cctgaaggta cgccgctcag cacgcagttg gtaaagtttc
     7741 agcgcaacaa aaagaaagtc ggcctggtcg tcaacgagta tggagacatt caggggctgg
     7801 tgacggttga agatattctg gaagagattg ttggcgattt cactacgtcg atgtcgccaa
     7861 cacttgccga agaggtcacg ccgcaaaacg acggttcggt gattatcgat ggcaccgcca
     7921 acgtgcggga aatcaacaaa gcctttaact ggcatctacc ggaagatgat gcccgcacgg
     7981 ttaatggcgt cattcttgag gcactggaag agatccctgt cgcaggcacc cgcgtgcgta
     8041 ttggcaagta cgatatcgat attctcgacg tacaggacaa tatgattaag caggtaaaag
     8101 tttttcctgt gaaaccgctg cgcgagagtg tggcggagta acgaaaacgg ccctgcattc
     8161 gcatgcaggg ccgtgaatta ttacgaaaac agaaattacg cttttgcttt cgctacagtt
     8221 accatcgccg cacgaatcgt acgaccattc agcgtataac ccttctgcat aatgcccagt
     8281 acgttacctg gcgcaacgtc atcagattcc accattgcga tagcctgatg cacattcggg
     8341 tccagcggca cgttagtttc ggcgatcact tcaacaccaa acttacgcac aacatccagc
     8401 atcgacttca gcgtcagctc aatgccttca accatcgcag acatatccgg gttagcttta
     8461 tcagccactt ccagcgcacg atccaggcta tcaatcaccg gcagcaattc gttgatgaat
     8521 ttctccagcg cgaatttgtg ggctttttca atatccagtt cagtacgacg acgcaggttt
     8581 tccatttcgg cttttacgcg caaaataccg tcacgttcac gggtctgggc ttcagccagc
     8641 tgagcttcga gattcgcaat tttttcatcg cgcggatcca cctgctcagc agaagcttct
     8701 ggctcaactg cctcaatctc ttcgtgctga tccatgataa tttcttccgg ggcttgcccc
     8761 tcaggcgttt tctgttcttt actactcatg aatttctccg cgtttttttc gcattcatct
     8821 cgctaacttc gcttattatg gggatcagtt tcagggtttc aagggaagca ctcacattgt
     8881 catcaatctt cgcaacaagg acctcggaaa aatgaataat catttcaagt gtattggcat
     8941 tgtgggacac ccacggcacc ctactgcact gacaacacat gaaatgctct accgctggct
     9001 gtgcacaaaa ggttacgagg tcatcgttga gcaacaaatc gctcacgaac tgcaactgaa
     9061 gaatgtgaaa actggcacgc tcgcggagat tgggcaactg gctgatctcg cggtagtcgt
     9121 tggtggcgac ggtaatatgc tgggcgcggc gcgcacgctc gcccgttacg atattaaagt
     9181 tattggaatc aaccgtggca acctgggttt cctgactgac cttgaccccg ataacgccca
     9241 gcaacaatta gccgatgtgc tggaaggtca ctacatcagc gagaaacgtt ttttgctgga
     9301 agcgcaagtc tgtcagcaag attgccaaaa acgcatcagc accgcgatta acgaagtggt
     9361 acttcatcct ggcaaagtgg cgcatatgat agagttcgaa gtgtatatcg acgagatctt
     9421 tgcgttttct cagcgatctg atgggctaat tatttcgacg ccaacaggct ccaccgccta
     9481 ttccctctct gcgggcggtc ctattctgac cccctctctg gatgcgatta ccctggtgcc
     9541 catgttcccg catacgttgt cagcacgacc actggtcata aacagcagca gcacgatccg
     9601 tctgcgtttt tcgcatcgcc gtaacgacct ggaaatcagt tgcgacagcc agatagcact
     9661 gccgattcag gaaggtgaag atgtcctgat tcgtcgctgt gattaccatc tgaatctgat
     9721 tcatccgaaa gattacagtt atttcaatac attaagcacc aagctcggct ggtcaaaaaa
     9781 attattctaa ttttacgcca gcctctttac tgtatataaa accagtttat actgtacaca
     9841 ataacagtaa tggtttttca tacaggaaaa cgactatgtt ggcacaactg accatcagca
     9901 actttgctat cgttcgtgag cttgagattg attttcatag cggcatgacc gtaataactg
     9961 gcgagaccgg tgcgggtaaa tctattgcaa tagatgccct cggtctttgt ctcggtggtc
    10021 gcgctgaagc cgacatggtg cgtaccggcg ctgctcgcgc tgacctgtgc gcccgttttt
    10081 ctctgaaaga tacgccagcg gccctgcgct ggctggaaga aaaccagctt gaagacgggc
    10141 atgaatgttt gcttcgtcgc gtgatcagca gtgatggtcg ctcccgtggt ttcatcaacg
    10201 gtacagctgt tcctctgtca caactgcgcg aactgggtca gttgctgatt cagatccatg
    10261 gtcagcacgc tcatcaatta ctcaccaaac ctgagcacca aaaattcctg cttgatggct
    10321 atgccaatga aacctctcta cttcaggaaa tgaccgcacg ttatcagttg tggcatcaaa
    10381 gctgccgtga cctcgcgcat catcaacagt taagtcagga acgcgccgcc cgtgcggaac
    10441 tgctgcaata ccaattaaaa gaacttaacg aatttaatcc gcagcccgga gagtttgagc
    10501 aaatcgacga agagtacaaa cgtctggcga acagcggtca attgctgacc accagccaga
    10561 atgcattggc attaatggcc gacggtgaag acgcaaacct gcaaagtcag ctttacacgg
    10621 ctaaacaact ggtgagcgaa ttgattggca tggacagcaa actgtccggc gtacttgata
    10681 tgctggaaga agctaccatc cagattgctg aagccagcga cgaactgcgc cactactgcg
    10741 atcgtctgga tctcgatccc aaccgactgt ttgaacttga acagcgcatc tcaaaacaga
    10801 tttcgctggc acgtaaacat cacgtcagcc ctgaggcatt accacagtat taccagtcgc
    10861 tactggaaga acagcagcaa ctggacgatc aggccgactc acaagaaacg ctcgcgctgg
    10921 cggtaacgaa acatcatcag caggcactgg aaaccgcgcg cgcattacac caacaacgcc
    10981 agcaatatgc agaagaactt gcacagctga tcaccgacag tatgcatgcg ctctcaatgc
    11041 cgcatgggca gtttacgatt gatgtcaaat atgacgagca tcacctgggc gctgacggtg
    11101 ccgatcgtat tgagtttcgg gtaaccacca acccaggtca gccaatgcag cctattgcca
    11161 aagtcgcatc cggtggtgaa ttgtcccgca tcgcactggc aatccaggtc atcacggcgc
    11221 gtaaaatgga aaccccggca ctgatttttg atgaagtgga tgtagggatt agcggtccaa
    11281 cagcggcagt tgtcggcaaa ctgctgcgtc aactcggcga atcaactcag gtgatgtgtg
    11341 ttacccacct gccacaagtc gcgggatgtg gtcatcaaca ctattttgtc agcaaagaaa
    11401 ccgatggtgc gatgacagaa acacatatgc aatctctgga taaaaaagcg cggttacaag
    11461 agctggcgcg cctgcttggt ggcagtgaag tcacacgtaa cacactggcg aatgcgaaag
    11521 aactgcttgc agcgtaaact tttttcctgc ttcacggtca gagtaaacag caaaacgccg
    11581 taagaccgga aagcaaaagg ttttaaagtg atgaaaggtc tattatcatc ggcatattac
    11641 agatgagcca cgtactgctc gggcccgaaa aggaatcaaa tcactatgcg ctgtaaaacg
    11701 ctgactgctg cagcagcagt actattgatg ttaaccgcag gctgttccac tctggagcga
    11761 gtggtttacc gtcctgacat caaccagggg aactatctga ccgctaacga cgtatccaaa
    11821 atacgtgttg gcatgacgca acaacaagtt gcgtacgcat tgggtacacc gctgatgtcc
    11881 gatccatttg gtacgaatac ctggttctat gtcttccgcc agcaaccagg tcatgaaggt
    11941 gtaacgcagc aaacgctgac gctgaccttt aacagtagcg gtgtgttgac caatattgat
    12001 aacaaacctg cgctgagtgg taactaataa taaagttgct ctcaaagacg ttaaaaaagg
    12061 tgctcaatga gcaccttttt tctgtctgtt atttattcgc tgatttttct gctcgttgcc
    12121 tgcgaagctc tttcggatcg gcaatgagag ggcgataaat ctccacccga tcgccatcat
    12181 gcacactatc gcttagtttt gccggacggc tgtaaatgcc gactttgttt ttagttaaat
    12241 cgatatcggt acgcaattcc agcaagccac tggcgcgaat agcttcttca accgtcgcgc
    12301 cctcctgcag cgtcactcgc tgcaggtatt gcttctcagg tagcgcataa gccacctcaa
    12361 cggcaatttt acctagcact gtaaacctct ttcgcacgaa ccgtaaaagc ctggaccata
    12421 ttagccgcca gctctttaaa cacgcgacca aaggcgagtt caatcaactt attggtaaac
    12481 tcaaagtcga gatgaaactc gatacgacac gcctcctggc tcagcggcgt aaacttccat
    12541 ccaccaatca atttcttgaa cggcccatcc accagattca taagaatact ttggttactg
    12601 gtcaactggt tgcgggtagt aaacgttttg ctgatcccag ccttagagac atctaccgcc
    12661 gcagtcatct gcccaggagt ggactccaga atccgacttc cggtacaacc tggcaaaaac
    12721 tgaggataag actgaacgtc attcactaac tgatacattt gctccgcgct gtagggtacc
    12781 agtgcggtcc ggctaatctg aggcataaca atttccatca acaaaaatcc aacaaataat
    12841 atcatttatc ctgctgtaaa aaaaacgcta tcccggcgct gggtaacatc gggttcatgc
    12901 taagatagag ccttgtcccc cgcaggattg atatggggta ttttcgattt cagattaccg
    12961 atgattcacg acgcttatga cgaagaaaaa agcacataaa cctggttcag cgaccatcgc
    13021 gcttaacaag cgcgcccgtc acgaatactt tatcgaagaa gagttcgaag cgggacttgc
    13081 cctgcaaggc tgggaagtta aatccctgcg cgcaggaaaa gccaatatca gcgacagcta
    13141 cgtccttctg cgtgacggag aggcatttct gtttggcgct aacatcacgc caatggccgt
    13201 ggcctccacg catgtggtgt gcgatcctac ccgtacccgc aagttacttc tcaaccagcg
    13261 cgaactggac tcattgtacg gtcgcgtcaa tcgagaaggc tataccgtag tggcgctctc
    13321 cctgtactgg aaaaatgcct ggtgcaaagt gaaaatcggc gtcgccaaag gtaaaaaaca
    13381 gcacgataaa cgttcagata tcaaagaacg cgaatggcag gtggataaag cacgcatcat
    13441 gaaaaacgcc caccgttaaa cctgcactcc aattattgac cagttcctca ccgcgcctcc
    13501 ctctccggcg gcgcgaatga acatcttatt ggctatcaca tccgacacaa atgttgccat
    13561 cccattgctt aatcgaataa aaatcaggct acatgggtgc taaatcttta acgataacgc
    13621 cattgaggct ggtcatggcg ctcataaatc tggtatactt acctttacac attggggctg
    13681 attctggatt cgacgggatt tgcgaaaccc aaggtgcatg ccgaggggcg gttggcctcg
    13741 taaaaagccg caaaaaatag tcgcaaacga cgaaaactac gctttagcag cttaataacc
    13801 tgcttagagc cctctctccc tagcctccgc tcttaggacg gggatcaaga gaggtcaaac
    13861 ccaaaagaga tcgcgtggaa gccctgcctg gggttgaagc gttaaaactt aatcaggcta
    13921 gtttgttagt ggcgtgtccg tccgcagctg gcaagcgaat gtaaagactg actaagcatg
    13981 tagtaccgag gatgtaggaa tttcggacgc gggttcaact cccgccagct ccaccaaaat
    14041 tctccatcgg tgattaccag agtcatccga tgaagtccta agagcccgca cggcgcaagc
    14101 cctgcgggct tttttgtgct ccgaatttgt cccgcgaagt ctgatgccaa ctaattaaat
    14161 ccgatccttt taggcacctt gttaggcact tcataaagct atattgtctt tgaggtgcct
    14221 aaaactatgg aaacccggca atggcaagac aaaccaaacc tctatccgtt aaggaaatcg
    14281 aatctgctaa acccaaggaa gcggactacg ttctctatga tggcgatggc cttgagctac
    14341 tcattaaatc cagcgggagt aaaatctggc agtttcgcta cattcgccct gtcaccaaga
    14401 cacgagcaaa gaaaagcata ggcccttacc cgtcagttac ccttgccgat gccagaaact
    14461 accgtgcaga gtcccgctca ctcctagcta aacagattga tccgcaggaa catcagcaag
    14521 aacaattgcg tagttcgctg gaagccaaaa ccaatacttt ccagctcgta gctgaacgtt
    14581 ggtggaatgt gaagaaagcc agtgtgacag aggactatgc ggaagatatc tggcgctctc
    14641 ttgaaagaga tgtctttcct gcgattggcg acgttagcgt tacagatatt aaagctcata
    14701 cactggttca ggccgtacaa ccggttcagg ccagaggagc actggaaacg gttcgtcgcc
    14761 tgtgtcaacg tattaatgag gtcatgatct atgcccagaa cacagggctg attgatgctg
    14821 ttcccagcgt taatatcggt aaagccttcg aaaagcctca gaaaaagaac atgcccagca
    14881 ttcgaccgga tcagctatct caactgatgc agacaatgcg aacagccagc attagccttt
    14941 ccacacgctg cctgttcatg tggcaacttc ttactattac ccgccctgcc gaagcggctg
    15001 aagctcgctg ggaagaagta gacatagaag cgcaagagtg gaagattccc gcagcacgca
    15061 tgaaaatgaa ccgcgaccat actgttccat tgtcagatga agcaatagct gtgctggaga
    15121 tgatgaaacc gctaagcggc aaccgagaat ttatctttcc tagccgtatc aagcccaacc
    15181 agcctatgaa cagtcagact gttaacgcct cactaaaacg cgcaggtttt ggtggtgtgc
    15241 ttgtttcgca tggtctgaga tcaattgcca gtacggctct taatgaacaa ggctttccgc
    15301 ctgatgccat tgaggcagca ttggcccatg tggataagaa tgaggttcgt cgtgcttata
    15361 accgcagcga ttacctggaa cagcgtcgcc cgatgatgca atggtgggca aattttgtga
    15421 tggcagcgga tcgtggaagc atgattgaag gtgggataaa aggaatgaag ctagttgggt
    15481 gactaaatag ctgcgcggca tagtgtccca ctttcagcca gcccttgcag gttaagccac
    15541 cagacagtaa acggcgggtg tcgcgttatt aggcgcagct atttagtagt aatattagga
    15601 ttggtgattc tgcactccag aagtt
//
LOCUS       DAZSIO010000089        15443 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZSIO010000089 DAZSIO010000000
VERSION     DAZSIO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.089"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..73)
                     /locus_tag="ACHX5T_002562"
     CDS             complement(<1..73)
                     /locus_tag="ACHX5T_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HFR8467716.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQL"
     gene            complement(171..1280)
                     /gene="anmK"
                     /locus_tag="ACHX5T_002563"
     CDS             complement(171..1280)
                     /gene="anmK"
                     /locus_tag="ACHX5T_002563"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR8467717.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1554..2021
                     /gene="slyB"
                     /locus_tag="ACHX5T_002564"
     CDS             1554..2021
                     /gene="slyB"
                     /locus_tag="ACHX5T_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR8467718.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="ACHX5T_002565"
     CDS             complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="ACHX5T_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR8467719.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2703..2939
                     /locus_tag="ACHX5T_002566"
     CDS             2703..2939
                     /locus_tag="ACHX5T_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR8467720.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2942..3799
                     /locus_tag="ACHX5T_002567"
     CDS             2942..3799
                     /locus_tag="ACHX5T_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR8467721.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3799..5811
                     /locus_tag="ACHX5T_002568"
                     /pseudo
     CDS             3799..5811
                     /locus_tag="ACHX5T_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="ACHX5T_002569"
     CDS             complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="ACHX5T_002569"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR8467722.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6414..7310)
                     /locus_tag="ACHX5T_002570"
     CDS             complement(6414..7310)
                     /locus_tag="ACHX5T_002570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR8467723.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7359..7598)
                     /locus_tag="ACHX5T_002571"
     CDS             complement(7359..7598)
                     /locus_tag="ACHX5T_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR8467724.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7701..8300
                     /gene="nemR"
                     /locus_tag="ACHX5T_002572"
     CDS             7701..8300
                     /gene="nemR"
                     /locus_tag="ACHX5T_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR8467725.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8337..9434
                     /gene="nemA"
                     /locus_tag="ACHX5T_002573"
     CDS             8337..9434
                     /gene="nemA"
                     /locus_tag="ACHX5T_002573"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR8467726.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9515..9922
                     /gene="gloA"
                     /locus_tag="ACHX5T_002574"
     CDS             9515..9922
                     /gene="gloA"
                     /locus_tag="ACHX5T_002574"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR8467727.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10025..10672
                     /gene="rnt"
                     /locus_tag="ACHX5T_002575"
     CDS             10025..10672
                     /gene="rnt"
                     /locus_tag="ACHX5T_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR8467728.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10765..15223
                     /locus_tag="ACHX5T_002576"
                     /pseudo
     CDS             10765..15223
                     /locus_tag="ACHX5T_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(15267..>15443)
                     /locus_tag="ACHX5T_002577"
     CDS             complement(15267..>15443)
                     /locus_tag="ACHX5T_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR8467729.1"
                     /translation="RLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGR
                     SLKQEPTEMIQATA"
BASE COUNT         3542 a         4083 c         4059 g         3759 t
ORIGIN      
        1 ccagttgatt aaaggcgcta caaccggaga gaaggaccgg caaaatgata attaacagtt
       61 ttttcatagt catatcccga agactttcct ggtccggagg gcaaataccc tccttaatct
      121 tccaagtgta acggcagaca cgataagaaa aaattcagtt aattctgata tcacgagttg
      181 gcggggaaaa tagcccccag taccgtctcc tggcttgcgc cagtgacgga aggcagattt
      241 cctggtaatc ccgccagcgt ccgccaggca agccaggcga aagccaatgc ttccatgtca
      301 tcgccactaa tgccaacggc atcggtggtg gtgacttctg tgcctagcag taataccgcc
      361 agacgcgcca tgagtagcgg attacgacta cctccaccac ataccatcaa tcgttcgcag
      421 ccaccgctca acaaaacttg ttcagaaatg gtcacggcgg tgagttctgc cagtgtggcc
      481 tgaacatcac ggggatcaac acccggaaaa tggcgtaaat ggcgctccag ccagccgtag
      541 ttaaagtatt cgcgtccggt gcttttcggt gcaggttgcg agaaatacgg gtcgctgagc
      601 atattttgca gcagtgggag aataacttta cccgcccgtg cccactcggc atctttatcg
      661 taaggtttac ctgcctgacg ccagatccag gcatccatca gcatgttacc aggaccggta
      721 tcgtagccgc caaccagctg cccaggaatg agcagtgaca gattggcgat gccgccaata
      781 ttgagcacca ttcgtcgctc ggttgggtga gccagcaagg catgatggaa cgcaggtacc
      841 agcggtgcgc cttgtcctcc caaggcaata tcgcgacggc gaaaatcacc gacaaccgta
      901 attccggtgc gtgccacaat ttgattgtta tcgccaatct gtaaagtgtg tggtgccacg
      961 cccgtcggtt catgccagac ggtttgaccg tgacagccga tcgcaactat atctctcgcc
     1021 tgcaggtttt gttccttaag caaggcattg accgcatcag caaaaagtcg cccgagttga
     1081 gtatcaagct gtccaaactg cgaaagtgta agctgctggc cctggcaaat atccagtaca
     1141 gcctgtttca gagacaccgg gatcggccag ctcaagcttg ccagctgtgc gacccggtgt
     1201 tcatcaattg tcgccaacac aacatcaaca ccatcaaggc tggtgcctga cataacgcca
     1261 ataaagcggc ccgatttcat agttcatcct ttttcaatct gacgtttgcg caccactcaa
     1321 acataaactt ttcgtgaata ccatgcggaa tgaccgattt ttaccgttgg tagtaaaaca
     1381 ttatcttcaa atcaataatc atcatgaatg ttttgtttat aattggttga tcctactttc
     1441 attatgattt gctcatattt ggtagaacat gtaaccatgg attcacatat gccatatact
     1501 ttgaccatga gggatgcttg cgtggcgttt catggtgaac aggagatttt tcaatgatta
     1561 aacgcgtatt ggttgtttca atggtaggtc tgtctcttgt cggttgtgtt aataacgaca
     1621 ccctgtcagg ggatgtttat accgcttctg aagcgaaaca agtacagaat gtcagctatg
     1681 gcaccatcgt taacgtacgt ccggtacaga ttcagggcgg tgatgattcc aacgttatcg
     1741 gtgcaattgg cggtgctgtt cttggtggtt tcctggggaa tactgttggt ggcggaaccg
     1801 ggcgttctct ggctactgca gcaggcgctg ttgcaggtgg cgtagctggt cagggcgtac
     1861 agagtgcaat gaacaaaacg cagggtgtcg agctggaaat tcgtaaagac gatggtaata
     1921 ccatcatggt ggtacagaaa caaggcaaca ctcgtttctc tccgggccaa cgtgtcgtac
     1981 tggccagcaa tggcagtcag gtgaccgttt ctccgcgcta aataagtttg cgtgtggtca
     2041 ggttactgac cacacgcccc cttcatttca ccctttggcc tgtaactcaa tgatattatg
     2101 ctcaagtttt gcgatgagcg taatcagttg ctccagttcc tctgcggaga tgccatgtaa
     2161 tatttccgcg cgggttttgt taataacagc ttccatttcg ctgatcagtg gctctgcctt
     2221 ttccgtcagt ttaatacgtt tagcccgacg atcgctggca caagtttgac gcgaaattaa
     2281 ccctttttct tccagttggt ccagagtacg gaccagtgat ggctgctcga tgccaatcgc
     2341 ttttgccagt tgaatttgcg actggtctgg aggtaactga tggatattgt gtaacgtaac
     2401 ccaatgggtt tgtgttaact ccagcggttt caggcgatgg tctatcagag cacgccatat
     2461 gcgcaccaac cgtgccagat cagaacctag tggcgattcc aatttcatct ccttataatt
     2521 agcttgctaa gatattatgc ggcttttaga atagtgtgca gcaattgtat tgctaaaaca
     2581 aatgtattgc tgcatttggt taccgtcaga catatttttc agaaattgcg cgtaaatttt
     2641 tcgcacttaa agaatattta ttaatctaac gcaatatatt cggtcgtaaa ggaatctact
     2701 ttgtgaagtt tatgctcaat gcaacaggat tgcccttaca agatctggtg ttcggtgcgt
     2761 ccgtctattt tcctccgttt ttcaaagcat tcgcgtttgg attcgtcatc tggcttgtcg
     2821 tacaccgcct gcttcgtggc tggatctacg ccggcgatat ctggcatccc ttgttaatgg
     2881 atttatcgct gtttgcgatt tgcgtttgcc ttgctctggc aatactgatt gcgtggtaac
     2941 tatgtcaatt aaaacaatta agtatttctc aacaatcatt gtagcgatag ttgcggttct
     3001 tgccggatgg tggctgtgga attattacat gcaatctccg tggacacgcg acgggaaaat
     3061 acgcgcagaa caggtttcta tcaccccaca ggtgtcggga cgtattgttg agctgaatat
     3121 aaaagacaac caactggtta acgcggggga acttctgctc acgatcgata aaacgccctt
     3181 tcagatcgcc gagctgaacg cacaggctca gctggcgaaa gcgcaatctg acctcgcaaa
     3241 agccaataac gaagcgaatc gtcgtcgtca tctctcacaa aattttattt ttgccgaaga
     3301 actcgatacc gctaacctca atgttaaagc gatgcaggcc agtgttaatg ccgcacaggc
     3361 gacgctgaaa cagacacaat ggcaactggc gcaaacggaa attcgcgccc cggtgagtgg
     3421 atgggtgact aacctcacta cccgcatcgg cgattacgct gacaccggga aaccgctgtt
     3481 tgcccttgtc gacagccact cgttttacgt cattggttat tttgaagaaa ccaagttgcg
     3541 ccatatccgc gaaggtgcac cagcacaaat taccctctat agtgataaca aaacgttaca
     3601 gggtcacgtt tccagtatcg gtcgggcgat ttatgatcag agcgttgaaa gtgactcaag
     3661 ccttattccg gatgtgaaac ctaacgtccc ctgggttcgt ctcgcccaac tcgttcccgt
     3721 ccgctttgcg ctggataaag ttccaggcga tgtcacgctg gtgtcgggca ccacctgtag
     3781 cattgccgta ggtcaataat gaacgcatcg tcatggtcct tgcgcaattt gccctggttc
     3841 agggccacgc tggcgcaatg gcgttatgcg ttacgcaata ccattgccat gtgtctggcg
     3901 ctgacggttg cctattattt gaatctggat gaaccctatt gggcgatgac ctcggctgca
     3961 gtggttagct ttcccaccgt tggcggtgtc atcagcaaaa gcctcggacg catggctggc
     4021 agtttactgg gagccattgc ggcactgctt cttgccggac atacgctcaa tgagccgtgg
     4081 ttttttctat tgagcatgtc ggcgtggctt ggcttttgta cctaggcctg tgcgcacttc
     4141 acgaataacg tcgcgtatgc atttcaactg gcgggctaca cggctgccat catcgccttt
     4201 ccgatggtta atattactga ggccagccag ctgtgggata tcgctcaggc gcgcgtttgc
     4261 gaggtgatag tcggtatttt gtgcggcggc atgatgatga tgatcctgcc tagcagttcc
     4321 gatgctactg cccttttaac tgcattgaaa aacatgcacg cccgattact ggaacatgcc
     4381 agtttactct ggcagcctga aacaaacgat gccattcgtg cagcacatga gggggtgatt
     4441 gggcagatac tgaccatgaa tttgctgcgt atccaggctt tctggagcca ctatcgtttt
     4501 cgccagcaaa acgcgcgcct taatgcgcta ctccaccagc aattacgtat gaccagtgtc
     4561 atctccagcc tgcgacgtat gttgctcaac tggccctcac cgccaggtgc cacgcgagaa
     4621 attctcgaac aattgctgac ggcgctcgcc agttcgcaaa cagatgctta caccgtcgca
     4681 cgtattatcg ccccgctacg cccgaccaac gtcgccgact accggcacgt cgccttctgg
     4741 cagcgactac gttatttttg ccgcctttat ctgcaaagta gtcaggaatt acatcgtctg
     4801 caaagcggtg tagatgatca tgccagactc gcacggacat ccgctctggc tcgtcatacc
     4861 gacaacgccg aagctatgtg gagcgggctg cgtacatttt gtacgttgat gatgattggc
     4921 gcatggagta ttgcatcgca atgggatgcc ggtgccaatg cattaacgct ggcagcaatt
     4981 agctgcgtac tctactccgc cgtcgcagca ccgtttaagt cgttgtcact gctgatgcgc
     5041 acgctggtgt tactttcgct attcagcttt gtggtcaaat ttggcctgat ggtccagatt
     5101 agcgatctgt ggcaattttt actgtttctc tttccactgc tggcgacaat gcagcttctt
     5161 aaattgcaga tgcccaaatt tgccgcactg tgggggcaac tgattgtttt tatgggttcg
     5221 tttatcgctg tcactaatcc cccggtgtat gattttgctg attttcttaa ctataatctg
     5281 gcaaaaatcg ttggcgttgc gctggcttgg ctagcgttcg ccattctgcg tccaggatcg
     5341 gatgctcgta aaagccgccg ccatattcgc gcgctgcgcc gggattttgt cgatcagcta
     5401 agccgccatc caacactgag tgaaagcgaa tttgaatcgc tcacttatca tcacgtcagt
     5461 cagttgagta acagccagga tgcgctggct cgccgttggt tattacgctg gggtgtagtg
     5521 ctgctgaact gttctcatgt tgtctggcaa ttgcgcgact gggaatcgcg ttccgatccg
     5581 ttatcgcgag tacgggataa ctgtatttca ctgttgcggg gagtgatgag tgagcgtggc
     5641 gttcagcaaa aatcactggc ggccacactt gaagaattac agcggatttg cgacagcctt
     5701 gcccgtcatc atcaacctgc cgcccgtgag ctggcggcaa ttgtctggcg gctgtactgc
     5761 tcgctttcgc aacttgagca agcaccaccg caaggtacgc tggcctcgta attacttaat
     5821 tacaccacag gcatagcgtt caccgccacc gcccagcggt ttaggttgat cggacatatt
     5881 atcgccgcca acgtggacca tcagcgcctt gtctttgatt tcatccagtg atttcagacg
     5941 aggcgcgatg acggcatcgg tagctttgcc gtcattattg acgaccagtg caggcagatc
     6001 gcctaaatgc ccggtacctt ctggcccttc atgtttaccg gtattttgtg gatcaagatg
     6061 cccgcctgcg gattccgcgg cgctggcttt gccatctttg gtggctggct ggcagcttcc
     6121 tttggcatga atatggaagc catgttcacc ggggggtaat gctttcagat cgggtgaaaa
     6181 ctccagacct ttatcggttt cagtaatggt gacgctacca attgactgcc caaccccttg
     6241 cgacgtgacg aggttcatct cgactttttc actggcagct tgtgcgccgg tcgcaacaac
     6301 cagcgccaga atggccagac taaaacgttt cataggacct ccgttcattg tgcattcctg
     6361 aataagtgta aaccagtgac acatttttga acggcggcta ttcctaaaag tgcttacggt
     6421 acgtcgtacc ccagtgcagc tttacggata cgaaaccatt gttgacgggt cattttcagt
     6481 gtttctgctt cgacagctgc ccgtacgcgc tcaattttac ctgagccgat aattggcagc
     6541 ggctgcgacg gtaaacgtaa tacccaggcg taaaccacct gttcaatcga gcccgcgttt
     6601 aactcctccg ccaccacagc cagttcatca cgcagcggct ggaaataatc atcattaaac
     6661 agacgaccac caccaaggca ggaccacgcc atcggacgaa cacgcagttg ttgtagttgg
     6721 tcgagcgtgc catccagcag taacggctga tgcaccgggg atatttccac ctgattagtg
     6781 gcaagggtaa acggcagacg tgattgcaac agggcaaatt gcgcaggcgt aaagttcgat
     6841 acgccaaaat gacgcacttt gccgctctga tgcagatgtt tgaacgcgtc cgccacttca
     6901 tcggcatcca ttaacgggtc tggtcggtgg attaacagca gatccagatg atcggtcgcg
     6961 agattaatta gcgactgttc ggcgctctta atgatgtgat tgcggtcagt gatgtaatga
     7021 ccaatgacgt tttcttcacg cgcggtcgtc gcgataccgc atttactgac gatttccatc
     7081 cgttcacgca ggtgaggtgc cagtttcagt gcctcgccaa acgccgcttc gcactgatag
     7141 ccaccataaa tatcagcatg gtccacggtg gtcacgccga gatccagatg ctcttcaata
     7201 aaactgacca gctggcgggc ggacatattc cagtccatca atcgccagta gcccatcaca
     7261 aaacgggaaa actccgggcc ttccggcgca atagtaatac gctgaaccat aatcgcttcc
     7321 tcttatcaga tataagagga gtatacgcaa gattaggttc aaaagagtga tggttgctcc
     7381 ggttcgtctg atgacgctgg cttatttgcg cgtaatttgc gcattaatcg ctgccgacaa
     7441 aggcgcagca cctcttgttt ttcgccatcg ctcattttat tccagttaaa acgctcatcc
     7501 cgactacgaa aacagccgcg acaaaacccg cgttcgtcag actggcaaat tccccggcac
     7561 gggctctgga cgggaaagaa ctctaattgc tccgccacct cgccctcctc agataagatt
     7621 attaccatta ttgaagctgt taatgtccaa agtagcaact ttgcttgcac tagaccaact
     7681 ggtctactac actccaacgc atgaacaaac acaccgaaca tgatactcgc gaacatctct
     7741 tggcgacggg cgagcaactt tgcctgcaac gtggattcac cgggatgggg ctaagcgaat
     7801 tactaaaaac cgctgaagtg ccgaaagggt ccttctatca ctactttcgc tctaaagaag
     7861 cgtttggcgt tgccatgctt gagcgtcatt acgccgcata tcaccagcga ctggctgagt
     7921 tgctgcaatc cggcgaaggt aactaccgcg accgcatact ggcttattac cagcaaacac
     7981 tgaaccagtt ttgccaacat ggaaccatca gtggttgcct gacagtaaaa ctctctgccg
     8041 aagtgtgcga tctgtcagaa gatatgcgca gcgcgatgga taaaggtgct cgcggcgtga
     8101 tcgccctgct ctcgcaggct ctggaaaatg gccgtgagaa ccattgttta accttttgtg
     8161 gcgaaccgct acaacaggca caagtgcttt acgcactatg gcttggcgcg aatctgcagg
     8221 ccaaaatttc gcgcagtttc gagccactgg aaaacgcgct ggcccatgta aaaaacatta
     8281 ttgcgacgcc tgccgtttag caggcatttt ttatcaccag acgaccggga gcctttatgt
     8341 catctgaaaa actgtattcc ccactgaaag tgggcgcgat cacggcggca aaccgtattt
     8401 ttatggcacc gctgacgcgt ctgcgcagta ttgaaccggg tgacattcct accccgttga
     8461 tggcggaata ctatcgccaa cgtgccagtg ccggtttgat tattagtgaa gccacgcaaa
     8521 tttctgccca ggcaaaagga tatgcaggtg cgcctggcat ccatagtccg gagcaaattg
     8581 ccgcatggaa aaaaatcacc gctggcgttc atgctgaaaa tggtcatatg gccttgcagc
     8641 tgtggcacac cggacgcatt tctcacgcca gcctgcaacc tggcggtcag gcaccggtag
     8701 cgccttcagc acttagcgcg ggaacacgta cttctctgcg cgatgaaaat ggtcaggcga
     8761 tccgcgttga aacatccatg ccgcgtgcgc ttgaactgga agagattccg ggtatcgtca
     8821 atgatttccg tcaggccatt gctaacgcgc gtgaagccgc ttttgatctg gtagagctcc
     8881 actctgctca cggttatttg ctgcatcagt tcctttcccc ttcttcaaac catcgtaccg
     8941 atcagtacgg cggcagcgtg gaaaatcgcg cacgtttggt actggaagtg gtcgatgccg
     9001 ggattgaaga atggggtgcc gatcgcattg gcattcgcgt ttcgccaatc ggtactttcc
     9061 agaacacaga taacggcccg aatgaagaag ccgatgcact gtatctgatt gaacaactgg
     9121 gtaaacgcgg cattgcttat ctgcatatgt cagaaccaga ttgggcgggg ggtgaaccgt
     9181 atactgatgc gttccgcgaa aaagtacgcg cccgtttcca cggtccgatt atcggcgcag
     9241 gtgcatacac agtagaaaaa gctgaaacgc tgatcggcaa agggttaatt gatgcggtgg
     9301 catttggtcg tgactggatt gcgaacccgg atctggtcgc ccgcttgcag ctcaaagctg
     9361 agcttaaccc acagcgtgcc gaaagtttct acggtggcgg cgcggaaggc tataccgatt
     9421 acccgacgtt gtaatccaac attgcgagcg gcgtaaagcc gccgctatac taaaacaaca
     9481 ttttgaatct gttagccatt ttgaggataa aaagatgcgt cttcttcata ccatgctgcg
     9541 cgttggcgat ttgcaacgct ccatcgattt ttataccaaa gtgctgggca tgaaactgct
     9601 gcgtaccagc gaaaacccgg aatacaaata ctcactggcg tttgttggct acggcccgga
     9661 aaccgaagaa gcggtgattg aactgaccta caactggggc gtggataaat acgaactcgg
     9721 cactgcttat ggtcacatcg cgcttagcgt agataacgcc gctgaagcgt gcgaaaaaat
     9781 ccgtcaaaac gggggtaacg tgacccgtga agcgggtccg gtaaaaggcg gtactacggt
     9841 tatcgcgttt gtggaagatc cggacggtta caaaattgag ttaatcgaag agaaagacgc
     9901 cggtcgcggt ctaggcaact aatctcctgc cgggcgtgaa ctcatcgcgc ccgcatcttt
     9961 actgcatcga caagtaatat ttgtcataat gcgcgctgca atttatccgt attaagagaa
    10021 tcagatgtcc gataacgctc aacttaccgg tctgtgcgac cgttttcgtg gtttttatcc
    10081 tgttgtgatc gatgttgaaa cagccggatt taacgccaaa accgatgcgc tgcttgagat
    10141 tgccgccatc accctgaaaa tggatgaaca aggctggctg atgccggaca ccacattaca
    10201 tttccacgtc gaaccatttg tcggcgcaaa tttgcaacca gaagccctcg ccttcaacgg
    10261 cattgacccg aacgatcccg atcgcggcgc ggtcagcgaa tacgaggcgc tgcacgaaat
    10321 ttttaaagtt gtacgtaaag gtattaaagc gagcggctgt aaccgcgcca ttatggtggc
    10381 gcacaacgcc aattttgatc acagctttat gatggccgcc gcagaacgcg cctcgctgaa
    10441 acgtaacccg ttccaccctt tcgccacttt tgacactgct gcactggccg ggctggcact
    10501 cggacaaacc gtattgtcaa aggcttgcca gaccgctggc atggacttcg acagcaccca
    10561 ggcgcactcc gcgctgtacg acaccgaacg cactgctgtg ctgttttgtg aaatcgtcaa
    10621 ccgctggaaa cgtctgggag gctggccgct acctgccgcc gaagaggtgt aatcgagtcg
    10681 atgcctgatg acatgcaatg attcaggcat ctatagtgag gctattccac gcatcaagca
    10741 tgatattcac ggggaatagc gttaatggca gataatccag acccttcatc gctcctgccg
    10801 gacgtgtttt caccggcgac ccgcgactgg tttcttcgcg cctttaaaca gccgaccgct
    10861 gtccagtcgc aaacctggca tgtggcggcg cgaggcgaac atgcgctggt gattgcaccg
    10921 actggctccg ggaaaacgct ggcagcattt ctctacgccc tcgatcggct cttccgcgaa
    10981 ggcggcgaag atacccgcga ggcgcataag cgtaaaacct cacgcatcct ctatatttca
    11041 ccgataaaag ccctgggcac cgacgttcag cgcaacttgc agatcccgtt gaagggtatt
    11101 gccgatgaac ggcggcggcg cggcgaaacg gtaattattg atgaagtcca cgcggtagcg
    11161 ggcagtaaac gtggtgcgca tctggcgtta agtctggagc ggctcgatgc gctgctccac
    11221 acctcagcac agcgaattgg cctttctgcc actgtgcgct cagccagcga tgtggcagca
    11281 tttcttggtg gcgatcgccc ggttacggta gtcaacccgc ccacaatgcg ccatccgcag
    11341 atacgaattg tcgtaccggt cgccaatatg gatgatgtct catcggtcgc cagcggcacc
    11401 ggcgaagaca gccatgccgg ccgggaaggc tccatctggc catatattga aacgggtatc
    11461 cttgatgaag tgttgcgcca tcgctcgacc attgtcttta ctaattcacg cgggctggcg
    11521 gaaaaactga cggcacgatt aaatgagctt tacgccgcac gcttacagcg ttccccgtct
    11581 atcgccgttg atgcggccca tttcgagtcg acctccggcg caacctctaa ccgtgtacaa
    11641 agtagcgacg tttttattgc ccgctcacac cacggctccg tctctaaaga acaacgagca
    11701 atcaccgaac aggcgctgaa atcgggtgaa ttacgctgcg tggtcgcaac ctccagtctt
    11761 gaactgggga ttgatatggg cgcggtggat ctggtgattc aggtggcaac gccgctttct
    11821 gttgccagtg ggttacaacg cattggtcgc gccggacatc aggttggcgg tgtatctaaa
    11881 gggctgtttt tcccccgtac ccggcgtgat ttagtcgatt ccgcagtcat tgtagagtgt
    11941 atgttcgcag gcaggctgga aaacctgaca ccaccgcata atcctctcga cgtccttgcg
    12001 cagcaaaccg ttgccgccgc ggcgatggat gcattacagg tagacgaatg gtactcccgc
    12061 gtacgccgta ccgcaccgtg gaaagatctg ccaagacgtg tttttgacgc cacgctggat
    12121 atgctttccg ggcgctatcc ctctggcgat ttttctgctt ttcgccccaa actggtctgg
    12181 aacagggaga ccgggatatt gaccgcccga cctggcgctc aattgttggc ggttaccagc
    12241 ggcggcacca ttccggatcg tggcatgtat agcgtgttat tacccgaagg tgaagaaaag
    12301 gccggttcgc ggcgggtggg tgaactggat gaggagatgg tatatgagtc gcgggtgaac
    12361 gacattatca ctctcggcgc tacctcatgg cggatccagc aaatcacccg cgatcaggtg
    12421 attgtgactc ctgctccggg tcgttctgcc cagctcccct tctggcgtgg tgaaggtaac
    12481 ggacgtccgg ctgaattagg cgagatgatc ggcgattttc ttcatttact ggcggatggc
    12541 gcgttctttt ccgggactat tcccccgtgg ctggcagaag aaaatacgaa cgccaatatt
    12601 caggggttga ttgacgagca acgcaacgcg acgggcatcg ttccggggag tcgccatctg
    12661 gtcctcgaac ggtgccgtga tgaaattggc gactggcgta ttattttgca ctctccctat
    12721 ggaagacggg tgcatgaacc ctgggcgctg gcgattgccg ggcgaataca tgccctatgg
    12781 ggcgctgacg cgtctgtggt cgccagtgat gacagcattg ttgcgcgcat tcctgacact
    12841 gatggtaaat tgcccgacgc cgcgattttt ttgttttaac cagaaaagtt gctgcaaatt
    12901 gtccgcgagg cggtaggcag ctcggcactt ttcgccgccc gttttcgcga atgcgccgcg
    12961 cgggcattat taatgccggg ccgcacgccg ggccatcgca ccccgctttg gcaacaacgg
    13021 ctgcgcgcca gtcagttgct ggaaatcgct cagggatatc cggattttcc ggtcattctc
    13081 gaaaccctac gcgaatgtct gcaagatgtt tatgatcttc ccgcactgga acgtttgatg
    13141 cgtcgcctga acggtggcga aattcaaata tccgatgtaa cgaccactac gccctcgcct
    13201 ttcgccacaa gtttattgtt cggctatgtc gcggaattta tgtaccagag cgacgccccg
    13261 ctggcagagc gccgggcatc cgtactgtcg ctggacagcg agttactgcg caatctactc
    13321 ggacaggtcg atccggggga attactcgac ccgcaggtca ttcgccaggt ggaagaagag
    13381 ttgcaacgac tggctcctgg tagaagagcg aaaggtgaag aaggattgtt cgacctgctg
    13441 cgcgaactgg ggccaatgac cgttgaagac ctggcgcaac ggcatacagg cagcagtgaa
    13501 gaggttgcgt cgtatctgga aaatcttctt gcagtaaaac gaatcttccc agcgatgatt
    13561 agcggacagg agcgtcttgc ctgtatggat gatgccgcca ggctgcgtga tgccctcggc
    13621 gtacggctac cagagtcatt gccagagatt tatttacata gagtcagtta cccgcttcgc
    13681 gacctctttc tgcgctatct ccgggctcat gctctggtca cggctgaaca actggctcat
    13741 gagtttagtc tcggtattgc cattgtcgaa gagcagcttc agcaactgcg tgaacagggt
    13801 ctggtgatga atctgcaaca agacatctgg gtgagcgatg aagtatttcg tcgtctgcgt
    13861 ttgcgctcgc tgcaagccgc cagagaagcg acgcgtcccg ttgcagccac gacctatgcg
    13921 cgattgctgc tggaacgtca gggcgtatta cccgccaccg atggtagccc ggcgctcttt
    13981 gcctcaacat ctccaggcgt ttatgagggc gtagatggag tgatgcgggt gatcgaacag
    14041 cttgccggag tcggtttacc cgcctcactc tgggaaagcc agatcctgcc tgcccgcgta
    14101 cgcgactatt cgccagaaat gctcgatgaa ttactggcaa ccggagcggt tatctggtcg
    14161 ggacaaaaaa aagctgggtg aagatgacgg cctggtggca ctgcatctac agaaatatgc
    14221 cgcagaatcg ttcactcccg ccgaagcgga tcaggcgaat cgttcggcgc tgcagcaagc
    14281 gataatcgct gttctggctg acggaggagc ctggtttgca caacaaatca gccaacggat
    14341 acgcgataaa atcgtcgaat cggttgattc ctcggcactg caagaggcgt tatgggcgct
    14401 ggtctggcaa ggcgtcatta ccagcgatat ttgggcaccg ttacgcgccc tcacccgcag
    14461 cagttccaac gcacgcacct caactcgccg cagtcaccgg gctcgtcgtg ggcgtcctgt
    14521 ctatgcacaa cccgtctcgc cgcgggtatc ttacaacaca ccaaatctgg ctggacgctg
    14581 gtcgttattg caggtggagc cactaaacga taccgaaagg atgctggcgc tggcggaaaa
    14641 tatgctcgac cgctacggca tcatcagtcg tcaggcggtg atagccgaaa atatccctgg
    14701 cgggtttcca tcgatgcaaa cactttgtcg aagtatggaa gactccgggc gaattatgcg
    14761 aggtcgtttt gtagaaggtc tgggtggcgc gcaattcgct gaacgtctga ctattgaccg
    14821 attgcgcgat ctggcgacac aagccacgca aacacgccac tatacaccag tggcgctctc
    14881 cgccaacgat ccggctaatg tgtggggaaa tcttctgccc tgggctgcac atccggcaac
    14941 gctggtacca acgcgtcggg cgggtgcgct ggtggtcgtt tctggcggca aattgttact
    15001 ctatctggcg caaggcggca aaaaaatgct ggtctggcag gaaaaggagg aattactcgc
    15061 cccagagatt ttccaggcgc tgaccaccgc actgcgtcgc gaaccaaggc tgcgctttac
    15121 gctaacagaa gtgaatgatc ttccggtccg gcaaacgccg atgtttacgc tgctgcgtga
    15181 ggcgggattt tcaagttcgc cacaagggct ggattgggga tagttgacat cctccacgcc
    15241 ctgaaagacg tggttccctg ctacgttcag gctgtcgcct gaatcatttc ggtgggttcc
    15301 tgcttcaacg agcggcctga ctgcaccatc tctccacagg caagaacggc gtgccccgcc
    15361 gctaaaatgt tacgagcgcc atttacatcg gcgttcgctg tatatccaca tacctggcat
    15421 ctgaatttac tttgtgacag gcg
//
LOCUS       DAZSIO010000090        15412 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZSIO010000090 DAZSIO010000000
VERSION     DAZSIO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.090"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..1177)
                     /locus_tag="ACHX5T_002578"
     CDS             complement(<1..1177)
                     /locus_tag="ACHX5T_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8467730.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     "
     gene            complement(1376..1460)
                     /locus_tag="ACHX5T_002579"
     tRNA            complement(1376..1460)
                     /locus_tag="ACHX5T_002579"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1424..1426),aa:Leu,seq:caa)
     gene            1656..2675
                     /gene="ahr"
                     /locus_tag="ACHX5T_002580"
     CDS             1656..2675
                     /gene="ahr"
                     /locus_tag="ACHX5T_002580"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR8467731.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2804..4306
                     /locus_tag="ACHX5T_002581"
     CDS             2804..4306
                     /locus_tag="ACHX5T_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR8467732.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="ACHX5T_002582"
     CDS             complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="ACHX5T_002582"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR8467733.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="ACHX5T_002583"
     CDS             complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="ACHX5T_002583"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR8467734.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6670..6720)
                     /gene="ytgA"
                     /locus_tag="ACHX5T_002584"
     CDS             complement(6670..6720)
                     /gene="ytgA"
                     /locus_tag="ACHX5T_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR8467735.1"
                     /translation="MSIIILATISTTQENK"
     gene            6874..8385
                     /gene="pepA"
                     /locus_tag="ACHX5T_002585"
     CDS             6874..8385
                     /gene="pepA"
                     /locus_tag="ACHX5T_002585"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR8467736.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8519..8962
                     /gene="holC"
                     /locus_tag="ACHX5T_002586"
     CDS             8519..8962
                     /gene="holC"
                     /locus_tag="ACHX5T_002586"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR8467737.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8962..11817
                     /gene="valS"
                     /locus_tag="ACHX5T_002587"
     CDS             8962..11817
                     /gene="valS"
                     /locus_tag="ACHX5T_002587"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR8467738.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11871..13067)
                     /locus_tag="ACHX5T_002588"
     CDS             complement(11871..13067)
                     /locus_tag="ACHX5T_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFR8467739.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13260..13763
                     /locus_tag="ACHX5T_002589"
     CDS             13260..13763
                     /locus_tag="ACHX5T_002589"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8467740.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13809..14225)
                     /gene="rraB"
                     /locus_tag="ACHX5T_002590"
     CDS             complement(13809..14225)
                     /gene="rraB"
                     /locus_tag="ACHX5T_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR8467741.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14387..15400
                     /gene="argF"
                     /locus_tag="ACHX5T_002591"
     CDS             14387..15400
                     /gene="argF"
                     /locus_tag="ACHX5T_002591"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR8467742.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3979 a         3933 c         3968 g         3532 t
ORIGIN      
        1 acatcaacct acgctcccca agatgttccg ccttatggat gtaagctgct cgcacagagc
       61 tacgctcctg gtgactcatc tgccgctcta ccgcatccct cgaccataat cccgactcaa
      121 tcaacgaact acaagccatc gtcctgaagc catgcccaca aacctcgacc ttcgtgtcat
      181 accccattac gcgcaaagcc ttgttcaccg tattctcact catcggctta cgtggatcgt
      241 gatcgcccac aaaaatcagc tctcgatttc cgctcatgct tttgatcttt tccaaaatac
      301 ttagggcctg ccgcgacaaa ggtacaaggt gtggtgtccg catcttcgag ccacgctgag
      361 aatgcttgac tccttccagt tgctcacgct cacctgggat tgtccacata gccgtttcaa
      421 aatctatttc tgaccagcgg gcaaaacgca gctcgctcga acggatgaaa accaataaag
      481 tgagttcaac cgccagtcga gttaacggtc tgccagaata gtgatctatg cgatgaagta
      541 attcaggtat acggttcagt tcaagagctg cacggtgctg tcttttcgcc gtagcaaccg
      601 cacctgcaat ctcttgcgcg gggttgtagt cgattaagcc gctctgaacg gcaaagcgca
      661 taatcgcggt agtacgctgc tgtaaacggg cggcaacttc gagccgcccg gatgactcga
      721 ctgctttaat gggtacaaga agatcccgcg tcttcagttc cgcaatgttc cgcttaccga
      781 tagcagtaaa gagattatcc tccagacttt tcaaaacacg agcgctatgc gatgccgacc
      841 acttctgatt gctggcgtgc cagtctctgg cgactacttc aaacgttatc gcctcttgtt
      901 cctgttctac cttaacagct ttcttgtttt cactgggatc gacaccatta gctaaaagct
      961 tacgagcctc atcccggcgt gccctggcat ccgccaacga cacttcgggg tatttcccca
     1021 atgccagcat cttctctttg ccgccaaagc gataacgtag ccgccagtat ttggagccgt
     1081 tagggtgaac cagcaaaacc atgccttcac cgtcggtcag cttataggct tttgcttcag
     1141 gcttagccga acgaaccttc acatcactca gagccatgat gagtatcctt tcaagggttc
     1201 tgtgtgggta caaacattat cgaaccggga aatacccgca gttgtacccg catcagtaag
     1261 ttgatgtaga ttgaatcagg ttgacttagg ttgagtgaaa aagcgaggaa agccttgcgg
     1321 atactggatt ttaggcacaa aaaaagacgt ccgttgacgt ctattgatgt tccgatggtg
     1381 ccgaaggccg gactcgaacc ggcacgtatt tctacggttg attttgaatc aactgcgtct
     1441 accgatttcg ccacttcggc actgaagagg tatgcggaaa acgttgtgga ttatacctgt
     1501 cgcacgccac catgcaagcg ccagcttgca cccacctcgc taagtgctga aattttcagc
     1561 attacactca gcgatatgta acctttgtca cactccaggc accccgccct gccatgctct
     1621 acacttccca aacaacacca gagaaggccc caaaaatgtc gatgataaaa agctatgccg
     1681 caaaagaagc gggcggcgaa ctggaagttt atgagtacga tcccggtgag ctgaagccac
     1741 aagatgttga agtgcaggtg gattactgcg ggatttgtca ttccgatctg tcgatgatcg
     1801 ataacgaatg gggattttca caatatccgc tggttgccgg gcatgaggtg attgggcgcg
     1861 tagtggcact cgggagcgcc gcgcaggata aaggtttgca ggtcggtcag cgtgtcggga
     1921 ttggctggac ggcgcgtagc tgtggtcact gcgacgcctg tattagcggt aatcagatca
     1981 actgcgagca aggtgcggtg ccgacaatta tgaatcgcgg tggctttgcc gagaagttgc
     2041 gtgcggactg gcaatgggtg attccactgc cagaaaatat tgatatcgag tccgccgggc
     2101 cgctgttgtg cggcggtatc acggtcttta aaccactgtt gatgcaccat atcactgcta
     2161 ccagccgcgt tggggtaatt ggtattggcg ggctggggca tatcgctata aaacttctgc
     2221 acgcaatggg atgcgaggtg acggccttta gttctaatcc ggcgaaagag caggaagtac
     2281 tggcgatggg tgccgataaa gtggtgaata gccgcgatcc gcaggcactg aaagccctgt
     2341 cggggcagtt tgatctcatt atcaatactg tgaacgtcag cctcgactgg cagccttatt
     2401 ttgaggcgct gacctatggc ggtaatttcc atacggtcgg tgcggttctc acgccgctgt
     2461 ctgttccggc ctttacgtta attgcgggcg accgcagcat ctctggttct gctaccggca
     2521 cgccttatga gctgcgaaag ctgatgcgct ttgccgcccg cagcaaggtt gcgccgacaa
     2581 ccgaactgtt cccgatgtcg aaaattaacg acgccattca gcatgtacgc gatggtaaag
     2641 cgcgctaccg agtagtcctg aaagccgact tctgatccct ttgccgggtg ctgtcgcccg
     2701 gcacttaaat ctctttggaa ggcgtctcgc aatctgctga atttagcggt gaagagtgca
     2761 cagccgttgc ctatactcca ggcaggaaac tggaggaaat ctcatgagcg aacccctgtt
     2821 aattgcccgc acgccggaca cagaactgtt tttactgccg ggaatggcta accgtcacgg
     2881 gctgattact ggcgcaacgg ggacgggtaa aaccgttacg ctgcaaaaac tggcagagtc
     2941 attgtcggaa atcggcgtgc cggtgtttat ggctgatgtg aaaggcgatc tgaccggtat
     3001 cgcgcaggca ggaacggcgt cggaaaaact gctcacaagg cttaaaaata tcggcgtcaa
     3061 tgactggcaa ccgcatgcca atccggtggt ggtgtgggat atctttggcg agaaaggcca
     3121 cacggtgcgg gcgacggttt cggatctggg gccgctgttg ctggcgcgac tgttgaatct
     3181 caacgatgtg caatctggcg tgctgaatat tatcttccgc attgcggacg atcagggatt
     3241 gttgctgctc gactttaaag atttgcgggc gattacccag tacatcggcg ataacgccaa
     3301 atccttccag aatcagtacg gaaatatcag tagcgcatcg gttggtgcca tccagcgcgg
     3361 attactgtcg ctggaacagc aaggcgcagc acacttcttt ggtgagccga tgctggatat
     3421 caaagactgg atgcgcaccg atgccaacgg taaaggcgtt atcaatatcc tcagcgccga
     3481 gaaactttat cagatgccga aactgtacgc cgccagcctg ctgtggatgc tttcagagtt
     3541 gtatgaacaa ttgccggaag cgggcgatct ggagaaacca aaactggtgt ttttcttcga
     3601 cgaggcacat ctgctgttta acgatgcacc gcaggtactg ctggataaga ttgagcaggt
     3661 gataaggctt attcgctcaa aaggcgtggg cgtctggttc gtttcgcaaa acccgtctga
     3721 tattccggat aatgtgttcg gccagctcgg taatcgtgtt cagcacgctt tgcgggcttt
     3781 tacgccaaaa gatcagaaag cagtgaagac agcggcgcaa accatgcggg ccaatccggc
     3841 atttgatacc gaaaaggcaa tccaggaact ggggaccggc gaggcgttaa tctcgtttct
     3901 cgatgcaaaa ggaagtcctt ctgtggtgga acgggcgatg gtgatcgcac cttgttcgcg
     3961 aatggggccg gtgacggaag atgagcgtaa tggcctgatt aatcactccc cggtgtatgg
     4021 caaatatgaa gatgaggtgg accgcgagtc cgcctatgag atgctgcaaa aaggctttca
     4081 ggccagtacc gagcagcaaa ataatccccc cgtgaaaggg aaagaggtgg cggtggatga
     4141 cggtattctt ggtggattga aggatatttt gtttggcact accggaccac gcggcgggaa
     4201 gaaagatggt gtggtgcaaa caatggcgaa aagcgccgct cgccaggtga cgaatcagat
     4261 tgtacgcggg atgttgggga gtttgctggg ggggagaaaa agataagtca gacgtgatct
     4321 gtgcccctca ccctaaccct ctccccaaag gggcgagggg accgttcgaa ctcgtatttg
     4381 actttgtcaa caataataaa gggagctttc gctcccttgt tagattacga ttttctcatt
     4441 aacagccaca ggctgattaa gaagaagctg gcacttggca acagtgcgcc gatgatcggc
     4501 gggatgccgt aaaccaacgt cagcgggccg aagatctggt ccagtacgta gaagacaaaa
     4561 ccgaagctga tacctgtgac cacacgcacg cccatcggta cgctacgcag tgggccaaag
     4621 ataaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa gattttgctc
     4681 cacatgttga gctgataacg tccggcatcc tgaccgctcg acttcagata cttcacgtag
     4741 ttgtgcaaac cactgatgga gagtgcatcc gggtccagcg ccaccacgcc cagtttgtct
     4801 ggcgtgaggt cggttttcca ggtgccgctc accgtctgcg aaccggtaat ctgtttcgga
     4861 ttggtcagat cagattcatc aacctgcgac agacgccaga ctttatgttc cgggtcaaac
     4921 ttcgcggtag cggcataacg tacggattgc agacgacgat tctcgttaaa ggcataaata
     4981 ctgatgccac ctaacacctc gtcaccttta acccgctcaa tgtagacgaa gttgttgcca
     5041 tctttcgccc ataaaccttg ctgggtagag agcaacgagc cgccgtacat cgcctgcgca
     5101 cggtagttac gcgccatctg ttcgccctgc ggcgcgaccc attcgccaat cgccatcgtc
     5161 agcaagacca gtggaatggc ggttttcatc accgacagcg ctacctgcat acgggtaaaa
     5221 ccagaagcct gcatcaccac cagttcgctg cgctgcgcca gcatcccaag accaagcaac
     5281 gccccaagaa gagccgccat cgggaagaag atctgcacat ctttcggcac gctcagcaag
     5341 gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt cagctgatcg
     5401 acaaatttga taatgcccga cagcgacacc agcatgaaca gcgtcatcat gatggtggtg
     5461 aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac accgctcctt
     5521 tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg ttgagaacaa
     5581 tcgccaaagc cagataaatc aggttaacgg tccacatcca cagtgtcggg tccagcttac
     5641 ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaaaagtaga tacagcagca
     5701 tggctggcag catcgacagt acgcgtccct gacgcgggtt aaccacgctc aacggtacaa
     5761 ccataagtgc catcataaac acggtgaata ccaacgtgat acgccagttc agttctgcgc
     5821 gagcacgatc ggtgtcagtg ttccacaatg tgcgcatgtc catctggtcg gtatcgttcg
     5881 ggtcgagcgc caccgcctgg tgaccaatga tcgcctgata atcctggaaa tccgtaatgc
     5941 ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga gtgacgacct
     6001 gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca gaaggacgtg
     6061 cattaccttt tggtcgaatt tgtgcgagga acacatcttt gaaatcgctg ccgtcaacgc
     6121 tttcgatgaa cagcaccgag ctgccattag tcgcttgctg gaattgccct tgcgccagcg
     6181 ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga cgcgatgacc
     6241 acggtcccgc ccacatcacg ttaaccgccg cgacgattgc cgtgaatacc gcaaggatca
     6301 ttgccgcttt caccagaacc gctttgctca agccgcaggc atgcattacc gtaatttcac
     6361 tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt aatggcagga
     6421 taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga ttcgccggaa
     6481 tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag aagatcaaaa
     6541 gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga tatcttatga
     6601 ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc ttgtttcatg
     6661 gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa ttatactcaa
     6721 cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt taagattaac
     6781 acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat ctgtagccac
     6841 cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa aagcggtagc
     6901 ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg tcgcctttct
     6961 ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct gctacgccgg
     7021 ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc accatgttcc gaatgtactt
     7081 tccgagcgaa ttctccttat tggttgcggc aaagaacgtg agctggatga gcgtcagtac
     7141 aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcgat ggaagcggtc
     7201 tgctttctga ctgaactgca cgttaaaggc cgtaacaact actggaaagt gcgtcaggct
     7261 gtcgagacag caaaagagac gctctacagt ttcgatcagt tgaaaacgaa taagagcgaa
     7321 ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga actgaccagc
     7381 ggtgagcgcg cgatccagca cggtcaggcg attgccgccg ggataaaagc agcgaaagat
     7441 ctcggtaata tgccgccgaa tatctgtaac gccgcttatc tcgcctctca ggcgcgccag
     7501 ctggctgaca gctacagcaa gaatgtcatc acccgcgtta tcggcgaaca gcagatgaaa
     7561 gagctgggaa tgcactctta tctggcggtc ggtcagggtt cacaaaacga atcgctgatg
     7621 tcggtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt gctggtgggt
     7681 aaaggtttaa ccttcgactc cggcggtatc tccatcaagc cttcagaagg catggatgag
     7741 atgaagtacg atatgtgcgg cgcggcggca gtttacggcg tgatgcgtat ggtcgcggag
     7801 ctgcaactgc cgattaacgt tattggcgtg ctggcaggct gtgaaaacat gcctggcggg
     7861 cgtgcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaaccgt tgaagtgctg
     7921 aacaccgatg ccgaaggccg cctggtactg tgcgacgtgt taacttacgt tgaacgtttt
     7981 gagccggaag cagtgattga cgtggcgacg ctgaccggtg cctgcgtgat cgcgctgggt
     8041 catcatatta ctggtctgat gacgaaccat aatccgctgg cccatgaact gattgccgcg
     8101 tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg gtgacgagta tcaggaacag
     8161 ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg ggcgattacc
     8221 gcaggttgct tcctgtcacg ctttacccgt aagtataact gggcgcacct ggatattgca
     8281 ggaaccgcct ggcgttctgg taaagcaaaa ggcgcaaccg gtcgtccggt agcgttgctg
     8341 gcacagttcc tgttaaaccg cgctgggttt aacggcgaag agtaagtttg cgttttaccc
     8401 tcaccctaac cctctcccta agggagaggg aaccgatcga gcgcaataga aattcattgc
     8461 tcatctttca gggtgaggaa tgcagcaata tttaaatcca ccacaagaag ccccatatat
     8521 gaaaaacgca acgttctacc ttctggacaa tgacaccacc gtcgatggct taagcgccgt
     8581 tgagcaactg gtgtgtgaaa ttgccgcaga acgttggcgc agcggtaagc gcgtactcat
     8641 cgcctgtgaa gatgaaaagc aggcttaccg gctggatgaa gccctgtggg cgcgtccggc
     8701 agaaagcttt gttccgcata atttagcggg agaaggaccg cgcggcagtg cgccggtgga
     8761 gatcgcctgg ccgcaaaaac gcagcagcag cccgcgcgat atattgatta gtctgcgaac
     8821 aagctttgca gattttgcca ccgctttcac agaagtggta gacttcgttc cttatgaaga
     8881 ttctctgaaa caactggcgc gcgaacgcta taaagcctac cgcgtggctg gtttcaacct
     8941 gaatacggca acctggaaat aatggaaaag acatacaacc cacaagatat cgaacagccg
     9001 ctttacgagc actgggaaaa gcagggctac tttaagccta atggcgatga aagccaggaa
     9061 agtttctgca tcatgatccc gccgccgaac gtcaccggca gtttgcatat gggtcacgcc
     9121 ttccagcaaa ccatcatgga taccatgatc cgctatcagc gcatgcaggg caaaaacacc
     9181 ctgtggcagg tcggtactga ccacgccggg atcgctaccc agatggtcgt tgagcgcaag
     9241 attgccgcag aagaaggtaa aacccgtcac gactacggcc gcgaagcttt catcgacaaa
     9301 atctgggaat ggaaagcgga atctggcggc accattaccc gtcagatgcg ccgtctcggc
     9361 aactccgtcg actgggagcg tgaacgcttc accatggacg aaggcctgtc caatgcggtg
     9421 aaagaagttt tcgttcgtct gtataaagaa gacctgattt accgtggcaa gcgcctggta
     9481 aactgggacc cgaaactgcg taccgctatt tctgacctgg aagtggaaaa ccgcgaatcg
     9541 aaaggttcga tgtggcacat ccgctatccg ctggctgacg gcgcgaaaac cgcagacggt
     9601 aaagattatc tggtggttgc gaccactcgc ccggaaaccc tgctgggcga taccggtgta
     9661 gctgttaacc cggaagatcc gcgttacaaa gatctgattg gcaaatatgt cattctgccg
     9721 ctggttaacc gtcgtattcc gatcgttggc gacgaacacg ccgacatgga aaaaggcacc
     9781 ggctgcgtga agatcacacc ggcgcacgac tttaacgact atgaagtcgg taaacgtcac
     9841 gccctgccga tgatcaacat cctgaccttt gacggtgata tccgtgaaag cgcccaggtg
     9901 ttcgatacca aaggtaacga atctgacgtt tattccagcg agatcccggc agagttccag
     9961 aaactcgagc gttttgctgc acgtaaagca gtcgttgctg cggttgatgc actcggcctg
    10021 ctggaagaaa ttaaaccgca cgacctgacc gttccttacg gcgaccgtgg cggcgtggtt
    10081 atcgaaccga tgctgaccga ccagtggtac gtgcgtgccg atgtactggc gaaaccggca
    10141 gttgaagcgg ttgagaacgg cgacattcag ttcgtaccga agcagtacga aaacatgtac
    10201 ttctcctgga tgcgcgatat tcaggactgg tgtatctctc gtcagctgtg gtggggtcac
    10261 cgtatcccgg catggtatga cgaagcgggt aatgtttatg ttggccgcaa cgaagaagaa
    10321 gtgcgcaagg aaaataacct cggtgccaac gttgccctgc gtcaggacga agacgttctc
    10381 gacacctggt tctcttctgc actgtggacc ttctctaccc ttggctggcc ggaaaatacc
    10441 gacgccctgc gtcagttcca cccaaccagc gtgatggtat ctggtttcga catcatcttc
    10501 ttctggattg cccgcatgat catgatgacc atgcacttca tcaaagatga aaatggcaaa
    10561 ccgcaggtgc cgttccacac tgtttacatg accggtctga ttcgtgacga cgaaggtcag
    10621 aagatgtcca aatccaaggg taacgttatt gacccgctgg atatggttga cggtatttcg
    10681 ctgccagaac tgctggaaaa acgcaccggc aatatgatgc agccgcagct ggcggacaaa
    10741 atccgtaagc gcaccgagaa acagttcccg aacggcattg agccgcacgg caccgatgcc
    10801 ctgcgcttca ccctggcggc gctggcgtct accggtcgtg acatcaactg ggatatgaag
    10861 cgtctggaag gctaccgtaa cttctgtaac aagctgtgga acgccagccg ctttgtgctg
    10921 atgaacacag aaggtcagga ttgcggcttc aacggcggcg aaatgacgct atcgctggcg
    10981 gaccgctgga ttctggcgga gttcaaccag actatcaaag cgtaccgcga agcgctggac
    11041 agcttccgct tcgatatcgc cgcaggcatt ttgtatgagt tcacctggaa ccagttctgc
    11101 gactggtatc tggagctgac caagccggta atgaacggtg gaacagaagc cgaactgcgc
    11161 ggtactcgcc atacgctggt gactgtactg gaaggtctgc tgcgcctcgc gcatccgatc
    11221 attccgttca tcaccgaaac catctggcag cgtgtgaaag tactttgcgg tatcactgcc
    11281 gacaccatca tgctgcagcc gttcccgcag tatgatgcat ctcaggttga tgaagccgca
    11341 ctggccgaca ccgagtggct gaaacaggcg atcgttgctg tacgtaacat ccgtgcagaa
    11401 atgaacatcg cgccgggcaa accgctggag ctgctgctgc gtggttgcag cgcggatgca
    11461 gaacgtcgcg taaatgaaaa ccgtggcttc ctgcaaacgc tggcgcgtct ggaaagcatc
    11521 accgtgctgc ctgccgatga caaaggtccg gtttccgtta ccaagatcat cgacggcgca
    11581 gagctgctga tcccgatggc tggcctcatc aacaaagaag atgagctggc gcgcctggcg
    11641 aaagaagtgg cgaagatcga aggtgaaatc agccgcatcg agaacaagct ggcaaatgaa
    11701 ggctttgtcg cccgcgcacc ggaagcggtt atcgcgaaag agcgtgagaa gctggaaggt
    11761 tatgcagaag cgaaagccaa gctgattgaa cagcaggctg ttatcgccgc actgtaatca
    11821 acacagtgat gaaaacgaag gccggagcat gctccggcct tttttatctc ttacagaaat
    11881 ggtaaagaag gcataactcc ccgagaaata ctggctaaga agcctttatc tggttgttct
    11941 tctttattga ttaaaggaag gtcatccaaa tcgcccagca aatacgtatt ttttgcctgc
    12001 cagtcaatca tccatatttt cagcagtggg taagccagac cacccgtaat cccggatatc
    12061 accaccaacg cacacatgcg ataaagcata ccgtggtacg ttaaagttga gcgaaaacga
    12121 atacgcccat cattcagtga caggttgctc ataaaatgat ttcgcaacga caccgttaaa
    12181 taactggtgc taacagcaat cccaaaataa taaattaact gcgcgattat cattttacgt
    12241 tgcatttcca taaattgctg taaattctca atatcatcat ttgcataccc agaactatca
    12301 tactcattta atatttgatc gaagataata taaccagcaa cagcgagaaa aggtaataaa
    12361 gctaaaaatg cgaggatggc atattttata caatacgcag tatctaattt caccttgaaa
    12421 cgatgtatac cgaaactggt attgctccag agaaaactca ttaccaggct atataaagta
    12481 ccattaaaaa taccaatgga aacaatacca actattgcca tcagaaccac agatataata
    12541 acactacttg aactattggc atgtagcatc tttgtcgaga taaagaaaac agtccccatc
    12601 ccaatggcca ttaaaatcgg caagaaaaag gttacccacc agaacccttt caaagagcaa
    12661 ttaaaactaa atcttacgcc gttgagactg gtcattaagg cctgataacg cagtcctttt
    12721 gctgccataa atataagcaa aaccaacagt aacaaagtca aaacacagcc aacaagcggc
    12781 atatctgctg acactgtcat taagattgcg aaatagaaaa aaacaaaaac aagacaacta
    12841 acaaaaacat tcccaccggt aattccataa gaaaatcgtt gtccgttaac ttccatatta
    12901 gcataaagat aacgcttaca tttcattaat gcccatggta aatagatacc tagcgttata
    12961 atcgttaaca acacattcac cacacaaata agaaaatatc tctcacctgt accatgaaaa
    13021 acaaaagaat gggacggaac atccatttca ttaataactt gagccataaa aatcccctga
    13081 attaaaaaaa ccattgatat aatttataga tacagacagt aaaatgttct gccacgttac
    13141 aatactctta tcagtaatag gctggcaaaa actttattgc tttataagac gcttgcgcac
    13201 gtaagaagag agtggtatta aacaaatgta taattacttt atttttattg ggttaaacta
    13261 tgaataacat tgcgccgcaa tcacctgtaa tgcgtcgcct gacgctgcag gataatcctg
    13321 ctatcgccag cgtcatccgt caggtttacg cagagtatgg tcttaccgcc gataaaggct
    13381 acaccgttgc cgatccgaat ctcgacgaac tgtatcaagt gtacagccag cctggccatg
    13441 cttattgggt ggttgaatac aatggcgaag tggtcggcgg cggcgggatt gcgccattaa
    13501 caggtagtga agcggacatt tgcgaactgc agaaaatgta ctttcttccc gctattcgcg
    13561 gtaaagggct agcgaaaaaa ctggccttaa tggcgatgga gcaggcacga gagatgggct
    13621 tcaaacgctg ctatctggaa acgaccgctt ttttaaagga agccattgcg ctttatgagc
    13681 atttgggctt tgagcatatc gactatgcgc ttggctgcac gggccatgtc gattgtgaag
    13741 tgcggatgct acgcgaactc taaaaaaatg cccgctagta tgtgctatcg ggcattttaa
    13801 tcaattaatt agtggcgaac cccgtcatcg tcttcatcga caaaatcttc atcgtcgcca
    13861 tcttcgccgt taggatcttc aaagtaagtg ccccaaccgt cgtactcaac gtcaaatttc
    13921 tccgccagcg tcatcagttg ttcaacctgg gcatcgatca gatcggcatt caacgcgcac
    13981 tcgctgagga tgtcgcagca aatcacgata tcaccatctt caacttccag ctcttctgga
    14041 tcggtcactt cgtaaccgag tttaaacgct tcaactgccg ctttttccag agtttccaga
    14101 tcgtctgcgg aaagatggtg ttcgatggtg tacagcgcgt ccggatcgct gccatcttcc
    14161 agtaattctt caataatcaa acgtgtttct tcacgctgtt cttccagttg ttccgggttt
    14221 gccatggctc attcctctta atgtcctgcc gatactctta ttgtcacaca ccactgacat
    14281 tgcctccacc tttgtgacaa agatttatgc tttagacttg caaatgaata ataatacaca
    14341 taaattgagt tttaattcat tgaggcgtta gccacaggag ggatctatgt ccgggtttta
    14401 tcataagcat ttcctgaaat tactcgattt cacgccagct gaactcaaca gcctgctgca
    14461 gttagccgcg aagctgaaag ctgataagaa aagcggtaaa gaagaagcca aactcactgg
    14521 taaaaacatc gcgctcatct tcgaaaaaga ctcgactcgt acccgatgct ctttcgaagt
    14581 tgccgcatat gaccaggggg ctcgcgttac ttatcttggc ccaagcggta gtcagattgg
    14641 tcataaagag tcgattaaag acactgcccg cgtgcttggt cgcatgtatg acggtattca
    14701 gtatcgcggc tatggtcagg agattgtcga aacactggcg gaatacgctg gcgtgccggt
    14761 atggaacggc ctgaccaatg agtttcaccc cacgcagttg ctggcggatc tcctcaccat
    14821 gcaggagcat ttacccggta aagcattcaa cgaaatgacg ctggtctatg caggtgacgc
    14881 acgtaacaac atgggcaatt cgatgctcga agctgcggcg cttaccggtc tggatttgcg
    14941 tctggtcgcg ccacaggcat gctggccaga agctgcgctg gttacggaat gccgcgccct
    15001 ggcacaacaa aatggcggga atattacgct gacggaagat gtagctaaag gagttgaagg
    15061 tgctgacttt atctataccg atgtatgggt gtcgatgggt gaagcaaaag aaaaatgggc
    15121 tgaacgcatt gcattactgc gtgattatca ggtgaacagc aagatgatgc agttgaccgg
    15181 taacccggag gtcaaattcc tccattgcct gcccgcgttt catgacgacc aaacgacgct
    15241 tggcaaaaaa atggcggaag agtttggcct acatggcgga atggaagtga ctgatgaggt
    15301 cttcgaatct gccgccagca ttgtatttga tcaggcggaa aaccgcatgc ataccatcaa
    15361 agcggtgatg gtcgcgacgc tcagtaaatt aaataattaa tatctctata aa
//
LOCUS       DAZSIO010000091        15127 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZSIO010000091 DAZSIO010000000
VERSION     DAZSIO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15127
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.091"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(50..2329)
                     /gene="maeB"
                     /locus_tag="ACHX5T_002592"
     CDS             complement(50..2329)
                     /gene="maeB"
                     /locus_tag="ACHX5T_002592"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR8467743.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDSLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2618..3568
                     /gene="tal"
                     /locus_tag="ACHX5T_002593"
     CDS             2618..3568
                     /gene="tal"
                     /locus_tag="ACHX5T_002593"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR8467744.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3588..5591
                     /gene="tkt"
                     /locus_tag="ACHX5T_002594"
     CDS             3588..5591
                     /gene="tkt"
                     /locus_tag="ACHX5T_002594"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR8467745.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5694..6737)
                     /locus_tag="ACHX5T_002595"
     CDS             complement(5694..6737)
                     /locus_tag="ACHX5T_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR8467746.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(6863..7438)
                     /gene="nudK"
                     /locus_tag="ACHX5T_002596"
     CDS             complement(6863..7438)
                     /gene="nudK"
                     /locus_tag="ACHX5T_002596"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFR8467747.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7506..9485)
                     /gene="aegA"
                     /locus_tag="ACHX5T_002597"
     CDS             complement(7506..9485)
                     /gene="aegA"
                     /locus_tag="ACHX5T_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR8467748.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9691..11408
                     /gene="narQ"
                     /locus_tag="ACHX5T_002598"
                     /pseudo
     CDS             9691..11408
                     /gene="narQ"
                     /locus_tag="ACHX5T_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            11610..14723
                     /gene="acrD"
                     /locus_tag="ACHX5T_002599"
     CDS             11610..14723
                     /gene="acrD"
                     /locus_tag="ACHX5T_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR8467749.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14822..14881)
                     /gene="ypfM"
                     /locus_tag="ACHX5T_002600"
     CDS             complement(14822..14881)
                     /gene="ypfM"
                     /locus_tag="ACHX5T_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR8467750.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3561 a         4009 c         3940 g         3617 t
ORIGIN      
        1 aaaccgctta ccctgaatat tcagggtaag cgtgagagtt aagaatgaat tacagcggtt
       61 gggtttgcgc ttcaaccacg gccagcgcca ccatattgac gatacgacgc accgatgcga
      121 tcggcgttaa cacgtgaacc ggtttcgcca cgcccatcag caccggaccg acagtcacac
      181 cttccgagct ggaaacgcgc agtaagttgt aactaatgcg ggcagcttcc atgttcggca
      241 tcaccagaat attggcagaa cctttcaaag ggctgtccgg catacggtcg ttgcgaatcg
      301 cttccaccag cgctgcatcg ccgtgcattt caccatcaat catcagatct ggcgcacgat
      361 ccctgaccag ttccagcgcc tgacgcattt tgctcgacga cgggcagtca gaagaaccaa
      421 agttggagtg cgacaacaga gcaacgcgcg gctcaatacc aaaacgacgg acagtttctg
      481 ccgccatcaa ggtgatctcc gccagctctt ctgcgtctgg ttcgtcattg acgtaggtat
      541 cggcaataaa ggtgttacca ctcggcagca gcagcgcatt cattgcacct gcggtgtgaa
      601 cgccatcgcg ataaccaaag acatttttca ccacactaaa atgttcatga taatcgccca
      661 ccgtaccgca gatcattgca tcggcttccc cacgctgaac catgatcgcg ccgatcactg
      721 tcgggttact gatcagcgca cgctgcgcct gttcctgagt gacaccgcga cgcttcatga
      781 tctggaagta ttcggtccag tactctttaa agcgcggatc ggattcgtta ttgacgattt
      841 caaaatcaac gcccgctttg atctgcaagc ccagtttctg aatgcgcatt tcgatcacgt
      901 tcggacgacc gataaggatc ggtttcgcca gtcccagcgt taccagttcc tgagtcgcat
      961 gcagaacgcg cgcctcttcc ccttccggca gaactacgcg cttcggcgct ttgcgagcct
     1021 gggagaaaat cggcttcata aacaggttgg ttttgtaaac gaactcagtc agcttgtcga
     1081 tgtagacgtc gaaatcagca atcggacgcg tcgccacgcc cgactccatc gccgcttttg
     1141 cgaccgcagg agcgatctta acgatcaagc gcggatcaaa cggttttgga atgatgtatt
     1201 ccggaccaaa gctcagatcc tgaacgccat acgctgaagc caccacttca ctctgttctg
     1261 catgggccag ttctgcaatc gcgcgtaccg ccgccagttt catctcttcg ttgatggcgg
     1321 ttgcgccaac gtccagcgcg ccacggaaga tgaacgggaa gcaaagaacg ttgttcacct
     1381 ggttcgggta gtcggaacgg ccggtacaga tgatggcatc cggacgcact tctttcgcca
     1441 gcggcggcag aatttccggt tccgggttcg ccagcgccag gatcattggt gcccgagcca
     1501 ttttcttcac catttcctgg gtcagcactt tcgggccgga acagcccagg aaaatatccg
     1561 caccttcaat cacatcatcg agggtacgtt tgccgtcatc caccaccgca tatgcggctt
     1621 tggtttccgc catatttggc tcacggccct gatagataac gccttttgaa tcgcaaacca
     1681 cgatgttatg tttttgcagc cccagcgcta ccagcaggtt catacaggcg attgctgcgg
     1741 cacccgcgcc ggaaaccacc atccgcacgt cggagatgtt tttctccacc acgcgcaagc
     1801 cgttgaggat ggcggcagtg ctgatgattg ccgtgccgtg ctgatcgtcg tggaataccg
     1861 gaatattcat ccgctcgcgc agtttctgtt caatatagaa acattctggc gctttaatgt
     1921 cttcgaggtt gatgccgccg aaggtcggtt cgagcgcggc gacgacttca ataaatttgt
     1981 ccgggtcgag ttcatcaact tcaatgtcaa atacatcaat cccagcgaat ttcttaaata
     2041 gaacgccctt gccttccatc accggtttgc ctgccagcgc gccaatgttg cctaacccca
     2101 gcaccgccgt accgttagag atcaccgcaa ccaggttacc gcgcgcggta tatttgtagg
     2161 cttttagcga gtctttttcg atttcaagac aaggtgcggc aacgcctggt gagtaggcca
     2221 gcgccagatc gcgctgcgtt gccagaggct tggttggaga aacctggatt ttccctggaa
     2281 ctggaaattc atggaaatca agtgcacttt gttttaactg gtcatccatt tggttgttcc
     2341 tttcacgtaa cgttcacaaa taaagtgtgt gggcaacagc ccctgcccac aacgtggcgc
     2401 acattattac cctgccggag tctacagact ttgagcaagt ccaaactctc accattaata
     2461 taatgttttg gtaataatcc tataacactg atgttacctg cttaatccag caataccatg
     2521 cctgtctgct atgctttttt gatgcgttta gcgaaatttc tcagaagtgt gaattaacgc
     2581 actcatctaa cactttactt ttcaaggagt atttcctatg aacgagttag acggcatcaa
     2641 acagttcacc actgtcgtgg cagacagctg cgatattgag tccattcgcc attatcatcc
     2701 ccaggatgcc accactaatc cttcgctgtt actcaaggct gccggattat cacaatatga
     2761 gcatttaata gacgatgcta tcgcctgggg taaaaaaaat ggcaagaccc aggaacaaca
     2821 ggtggtcgca gcgtgtgaca aactggcggt caatttcggt gctgaaattc tcaaaatcgt
     2881 acccggttgc gtgtcaacag aagttgatgc acgcctctct tttgataaag aaaagagtat
     2941 tgagaaggcg cgccatctgg tggacttgta tcagcaacaa ggcgttgaga aatcacgcat
     3001 tttgatcaaa ctggcttcga cctgggaagg aattcgcgcg gccgaagagc tggaaaaaga
     3061 aggtattaac tgcaacctga cgctgctgtt ttcttttacc caggcgcggg cctgtgcgga
     3121 agcaggcgtt tttctgattt cgccgtttgt agggcgtatt tatgactggt atcaggcacg
     3181 caagccgatg gacccgtatg tggtggaaga agatccgggc gttaaatcgg tgcgcaatat
     3241 ctacgactac tataagcaac accactatga aaccattgtg atgggcgcga gcttccgtcg
     3301 caccgaacaa atcctcgcct taaccggctg cgatcgactg actatcgcac cgaatttgct
     3361 gaaggagctg caggaaaaag tttctccagt ggtacgtaaa ttaatcccgc cttctcagac
     3421 gttcccacgc ccagcaccca tgagcgaagc ggagttccgt tgggagcaca atcaggatgc
     3481 gatggcggta gaaaaactgt ctgaaggcat tcgtctgttc gctgttgatc aacgcaaact
     3541 ggaagatctt cttgccgcca aactataaac cagccacgga gtgttatatg tcccgaaaag
     3601 accttgccaa tgcgattcgc gcactcagta tggatgctgt acaaaaagcc aattctggtc
     3661 atcccggcgc gccgatgggc atggctgata ttgccgaagt gctgtggaac gattttctta
     3721 aacataatcc taccgaccca acctggtatg atcgcgaccg ctttattctt tccaacggtc
     3781 acgcgtcgat gctgctctac agtttgctac atctgaccgg ttacgacctg ccgctggaag
     3841 aactgaagaa cttccgtcag ttgcattcga aaaccccagg ccacccggag attggctata
     3901 cgccaggcgt tgaaaccacc accggcccgc ttggacaagg tttggcgaac gccgtcgggc
     3961 tggcgatagc agagcgtaca ctggcggcgc agtttaacca gccagaccat gagatcgtcg
     4021 atcacttcac ctatgtgttt atgggcgacg gctgcctgat ggaaggtatt tcccacgaag
     4081 tctgttcgct ggcaggcacg ctgggactag gcaagctgat tggtttttac gatcacaacg
     4141 gtatttccat cgacggtgaa acagaaggct ggtttaccga cgatacggca aaacgttttg
     4201 aagcctatca ctggcatgtg atccatgaaa tcgacggtca cgatccgcag gcggtgaagg
     4261 aagcgatcct tgaagcgcaa agcgtgaaag ataagccgtc gctgattatc tgccgtacgg
     4321 tgattggctt tggttcgccg aataaagcag gtaaggaaga ggcgcacggc gcaccactgg
     4381 gggaagaaga agtggcgctg gcacggcaaa aactgggctg gcaccatccg ccatttgaga
     4441 tccctaaaga gatttatcac gcctgggatg cccgtgaaaa aggcgaaaaa gcgcagcaga
     4501 gctggaatga gaagtttgcc gcctataaaa aggctcatcc gcaactggca gaagagtttc
     4561 cccgacggat gagcggtggt ttaccgaagg actgggagaa aacgactcag aaatatatca
     4621 atgagttaca ggcaaatccg gcgaaaatcg ctacccgtaa ggcttcgcaa aatacgctta
     4681 acgcttacgg gccgatgctg cctgagttgc tcggcggttc ggcggatctg gctcccagca
     4741 acctgaccat ctggaaaggt tctgtttctc tgaaggaaga tccggcgggc aactacattc
     4801 actacggggt gcgtgaattt ggcatgaccg ctatcgccaa cggcatcgcg caccacggcg
     4861 gctttgtgcc gtataccgcg acgttcctga tgtttgttga atacgcccgt aacgccgcgc
     4921 ggatggcggc actgatgaaa gcgcggcaga ttatggttta tacccacgac tcaattggcc
     4981 tgggcgaaga tggtccgacg caccaggctg ttgagcaact ggccagcctg cgcttaacgc
     5041 caaatttcag cacctggcga ccgtgcgatc agatggaagc ggcggtgggc tggaagctgg
     5101 cggttgagcg ccacaacgga ccgacggcac tgatcctctc aaggcagaat ctggcccagg
     5161 tggaacgtac gccggatcag gttaaagaga ttgctcgtgg cggttatgtg ctgaaagaca
     5221 gcggcggtaa gccagatatt attctgattg ccaccggttc agagatggaa attaccctgc
     5281 aagcggcaga gaaattagca ggagaaggtc gcaatgtacg cgtagtttcc ctgccctcga
     5341 ccgatatttt cgacgcccag gatgaggaat atcgggagtc ggtgttgcct tctaacgttg
     5401 cggctcgcgt ggcggtggaa gcaggtattg ccgattactg gtacaagtat gttggtctga
     5461 aaggggcaat tgtcgggatg acgggttacg gggaatctgc tccggcggat aagctgttcc
     5521 cgttctttgg ctttaccgcc gagaatattg tggcaaaagc gcataaggtg ctgggagtga
     5581 aaggtgcctg atggtgatcg ccggatgctg tatgttcccc tcaccctaac cctctcccca
     5641 gaagggcgag gggacggtcc gtgcgctttt tagggccgca tctgcgattt tgtttaacgg
     5701 gtgatccaca acgtgggcct ggcgtctggc ccatgccagt tatcgcaggt aggttctgcg
     5761 gcgtaacgca ccaggcgaaa acgctgaccg tcaaagcgcc agcgcgcctg aatgccacaa
     5821 tcgcttaatc cgcgcccttt cgctaaggtc accagttcac gcgatttctc atcaaatgtt
     5881 gcgttcatca gttccagttc attcgtctcc tggccgttgt tgaacggcaa acgcaaccga
     5941 accgggcgcg aggctagcgg ctttttacgc gacacaatcc atgccaaatc aatggtgtta
     6001 taggcccctg cctcacagct aatcatcatc agtgctttat catcggtcag cgcagtgaca
     6061 ttcacctcac gacgtaacgg atcaagcgag cagcgcagac cattcatccg ccagtttcca
     6121 taatccagca aatcgttacg ttcttcgagt gagagtggcg tcggcgttgg gttaaccacc
     6181 gcgacctctt tcagcgcagg cgcgggcggt acgctgagag gcggttcgtc ccctttcttg
     6241 atccacgcgg tttcactgcc aacgcgcttt tgctgagcat caataaacaa caacgccgct
     6301 tttaagccgc tcagagaaat ggtctgattg ccgtcgcgta aggtgatcgc cttcccttcc
     6361 tgaattatct gcaaaaacgc ggtgatggtt gccgtatcat cggttactaa taaccatggt
     6421 gaaatccgcc acttgtcacc acttaatgcc aacggctcac catctaatag cagccgtggc
     6481 gctatctccc cttcagacgc atccggcgac ttcaacccac cgcgctcaat acgtaaaacg
     6541 gcatcggtat gcgccccagc gctgcggctc agggtcatca ccagtccatt atgatcgccc
     6601 gtattgcgcg ccacacaaaa attttgatta ttacaggtga cctgccagtc ggaaaacgcc
     6661 cgttgcgctg gtgccgccca caccaaagac gtcggcaaca aagcgaaaaa aaagagaagg
     6721 aaaatgcgat agcgcatgga cggtacgacc ccagaagttc aaacaaacag cacaagtcgt
     6781 atcttcgttg cgcaggtagc gcggctcaat cggatttgtc ggattgagcc aataataaac
     6841 aacgaaatat tattgctatt tttcagtcca ttaaatgtga catttgcaaa tagttaagca
     6901 ataacaccgt cttaccgtca cgtatctcgc cggttttgat catctccagc gcctggctga
     6961 acggcagctc gagcacttca atatcttcat cttcaacacc gccaccggcg ttagcgcgct
     7021 gattgtcact gtattcggcg ataaaaaagt ggattagctc agtcacaccg ccaggcgaca
     7081 tatacagttc aaataatttg cgcacttcgc ccacttcata gcccgtctct tcaatcgctt
     7141 ctttacgaat gcacacttcc ggttcgtcgt tatccagcag cccggcgcag gtttcaatca
     7201 gctgcccgct ttcattgcca ttaacccagg tagcgacacg gaactgacga atcagaacca
     7261 cgctcttttt cttcgcgttg tacaggagga tcgtcgcacc attaccgcga tcgtaaactt
     7321 cacgtttatg gcggataact tcgccatctt tgcgcgtgag atcgtaagta atgttgtgca
     7381 gggtgaaata gttatcggag agaattttgt ctttaatgag ggtgatttgt tgcgtcatac
     7441 cgactccaca gcgcgaaatg aacaattata ttacgctgtg aagtcgggtt tgtctgcgca
     7501 ggctatcagt gagatttgac tgattttacc cccagccagt caataatccc ctgtgccgca
     7561 tgacgtcctt ctgccattgc ggtaaccacc agatccgcac cacgcacggc gtcgccaccg
     7621 gcgaagattt tcggattggt ggtctggtaa cggtactggc tttccacatc cgcgatgatg
     7681 cggccccatt tgtctaccgt tacaccgtgc gactccagcc acggcatccc gtgtggattg
     7741 aagccaaacg ccataatcac cgcatcggct ggcatgacaa attcactgcc ctcaatcggt
     7801 acaggtcgcc gacgcccctg ggcatccggc tcgccaagac gcgtgcgcag gaaacgaatt
     7861 ccgcaaacgt gaccttgctc attcagctca agcgccaccg gctggacgtt aaattcgaag
     7921 ttggccccct cttcgcgggc gttcttcacc tctttcttcg agcctggcat gttagcttca
     7981 tcacgacgat aagcgcaggt gacgttactc gcgccgtggc gcagcgcagt acgcacacag
     8041 tccatcgcgg tgtcgccgcc gcccagtacc acgacgttaa gtccggcggt attgataaac
     8101 ggctcttccg gtagctcttc gagccccatc acctgtttag tgttggcaat gaggaacggc
     8161 agcgcgtcat aaacgcccgg cgcatcttca ttgggtaaac ccgctttcat ggaacggtaa
     8221 gtgcctacgc caacgaagac cgcgtcgtat tgttccaaaa gcaaatccaa agagacatct
     8281 ttacccactt cacaattgag ttcgaagtga atccccatcg cgctgaagat ttcccgacgg
     8341 cgtgccagca gggatttatc cagtttgaaa gaaggaatgc cgaaagtgag caagccaccg
     8401 atttctggat ggcgatcgta caccgtcacc gccacgccat tgcgggtcag aacatccgca
     8461 caggccagcc ctgccggacc tgccccgata atcgccaccc gcttgtccac tttggtgaca
     8521 tgacttaagt caggacgcca acctttcgcc aacgcctgat ctgaaatata gcgttcaatg
     8581 ttgccgatag ttaccgcgcc gtgctcatcg cgaatagtac aggcaccttc acacaaacgg
     8641 tcttgcggac aaacgcgtcc ggtaatttcc ggcagggtgt tggtctggtg agaaagctcg
     8701 acggcggcgt cgatgtttcc ggctttcacc agttcaatcc actgcggaat atggttatgc
     8761 agcgggcagg tccattcaca aacgctgtgc tcgccgaact taaggcagcg cgaggcttcc
     8821 cgttgtgcct ggtcggcgcg aaatggcaga taaatttcat caaaaccggt tttgcgcgct
     8881 tcaatcgcca gtttatccgg ctcgccacgc gcgggcgttg cctgcatttg ttcgacttta
     8941 ctcattaccg gcatttcttg tgcagcggta ctggcatgcc acggttgatg ctcctgacgt
     9001 gcggtgcgca agcggcggga tttcgccatg ccggagagtg cgacgtcagt gaccagttgc
     9061 agcgcgtccg ccgggcaatt ctcgacacag gcaggaccgt tttcgcgccc tgcacaaagg
     9121 tcgcatttat gcgccgtggc ttttactttt cctgccgcga cgggtgtcag gacgatttgc
     9181 atcgtaccaa acggacaggc caccacgcag gatttacagc caatacactt ttgctgattg
     9241 acctgaatgc tgtcatcaac gtggctgatt gcgccattag ggcagctacg ggcgcagggc
     9301 gcatcttcac aatggtgaca ggtcactgca ctacgttgct gctgatgttt gataaccgta
     9361 attcggggat gaaaatggtg ttggctcagg acatgttgct catcattgtg agccatgaca
     9421 caggcgattt cacaagcatg acaacccaga cattgctgac tgttggccat aataaaacga
     9481 ttcataacga ccttcttttt tggttgtaaa aaccttattc tttatatgag tgttgttatt
     9541 acccgactta caggggaacc ggcaatgttc atgtgcccag aataagtaac tatttcgcta
     9601 taaactgtgg cagatcaaat aatcccatca ctgactaaat tgcgtttcag cgaactggaa
     9661 cattaatgat tttttgtgga gaagacgcgt gtgattgtta aacgacccgt ctcggccagt
     9721 ctggcccggg ccttttttta cattgtgctg ctgtcgattc tttccacggg tatcgctctg
     9781 ctaactctgg cgagcagttt gcgcgacgct gaggctatca atattgccgg atcgctgcgt
     9841 atgcagagtt accgcctggg ctacgacttg caaagtggca gtccacaact caatgcacat
     9901 cgccagttgt ttcagcaggc actgcattca ccggtattaa ccaacctcaa cgtctggtat
     9961 gtgccagaag cagtaaaaac tcgctatgcg catctgaatg ccaactggct ggagatgaat
    10021 aatcggctca gcaagggcga tttgccgtgg tatcaggcca atattaataa ttatgttaat
    10081 cagatagacc tgttcgtgct ggctttacag cactacgctg aacgcaaaat gctgctggtg
    10141 gtggcgattt ccctggctgg cggtatcggt attttcacgc tggtcttttt tactttgcgc
    10201 cgcatacgcc atcaggtggt tgccccgctg aatcagctgg ttaccgccag tcagcgtatt
    10261 gaacacgggc agttcgactc gccgccgctg gataccagtc tgccgaatga gcttggtctg
    10321 cttgcaaaaa cctttaacca aatgtcgagc gagctgcata aattgtacct ttcgctggag
    10381 gcgtcagtag aagaaaagac ccgcgatctc cacgagacca agcgtcgtct ggaggtgttg
    10441 tatcagtgca gtgttcccag gcgctgaaca ccagccagat tgatgtgcat tgtttccgcc
    10501 atattttgca gattgttcgc gacaatgaag cagctgaata tctggagctg aacgttgggg
    10561 ataactggcg gattagcgaa gggaagccaa accctgagct gccaatgcag attttaccgg
    10621 tgacaatgca agagacggtt tacggcgaac tgcactggca aaatagtcac gtttcatcat
    10681 cagaaccgct gcttaacagt gtttcatcga tgctgggacg cggtttgtac tttaatcagg
    10741 cgcagaagca ttttcagcag ttattgttga tggaagaacg tgcgaccatc gcccgcgaat
    10801 tgcacgactc gctggctcag gtgctttctt acttacgtat ccagttgacg ttactgaagc
    10861 gttcgatacc ggaagataac gccaccgcac aaagtatcat ggccgatttt tcccaggcgt
    10921 tgaatgatgc ttatcggcag ttacgcgagc tgttgactac tttccgcctg acgctgcagc
    10981 aggcggatct cccctccgcg ttgagggaaa tgctggatac gttacaaaat caaaccagcg
    11041 ctaaactgac cctcgactgc cgtctgccaa ccctggcact ggatgcgcaa atgcaggtgc
    11101 atttgttgca aattattcgc gaagcggtgc tgaatgcgat gaagcacgcc aacgccagcg
    11161 aaatcgccgt cagttgcgtc accgcgccgg acggcaatca cacggtttat atccgtgata
    11221 acgggattgg tatcggtgaa ccgaaagaac ccgaaggtca ttatggactg aatatcatgc
    11281 gcgaacgcgc ggaacggctg ggcgggacgc tgactttttc acaaccttcc ggcggcggca
    11341 cgttagtgag tattagcttt cgctccgcag agggtgaggg gaaggggaac acgccggctg
    11401 aggtgtaaat aattaccctc acaccggccc tctcccttta aaggcgagag ggaaaaacgt
    11461 gcccatattg acctgtaagc gagtgtgtca gcaatctcat gataatttac attaactcct
    11521 ttttttctcc acgattggct cgtaccttgc cgctacagtg aagcaagtca agcctacaac
    11581 aatacgcaga aacacgaggt cctcttttaa tggcgaattt ctttattgat cgccccattt
    11641 ttgcctgggt gctgacaatc ctgttgtgtc tgacaggtac cctggcgatt ttttcattgc
    11701 ccgttgaaca ataccccgat ctcgcgccac cgaatgtgcg agtgaccgct aactatcccg
    11761 gcgcatcggc ccagacgctg gaaaacaccg tgacccaggt tatcgagcaa aatatgaccg
    11821 gcctcgataa tctgatgtat atgtcatctc agagcagtgg caccggtcag gcatctgtca
    11881 ctttaagttt taaagcaggc accgatccgg acgaagccgt gcagcaagta caaaaccagc
    11941 tgcaatcagc catgcgaaag ttaccgcagg cggtgcaaaa tcagggcgtg acggtgcgta
    12001 aaaccggcga taccaacatt ctgaccattg ccttcgtctc taccgatggc tcgatggata
    12061 aacaggatat tgctgattat gttgccagta atattcagga cccgttaagc cgcgtgaatg
    12121 gcgtcgggga tatcgatgcc tatggttcgc aatattccat gcgtatctgg ctggacccgg
    12181 cgaaactcaa cagtttccag atgacggcta aagatgtcac tgatgccatt gagtcacaga
    12241 acgcgcagat tgcggttggg caacttggtg gtacaccttc cgtcgataag caggcgctca
    12301 acgccaccat taacgcccag tcactgctgc aaacaccaga acagttccgc gatatcacct
    12361 tgcgggtgaa tcaggacggc tcagaggtaa ggctgggcga tgtcgccacc gtcgaaatgg
    12421 gggcggagaa atacgattat cttagccgct tcaatggtaa gccagcctcc gggttggggg
    12481 taaaactggc ctccagcgca aacgaaatgg cgacagcgga gctggtgctc aatcgtctcg
    12541 acgagctggc gcagtatttc ccgcacggac tggaatacaa ggtggcgtat gaaaccacct
    12601 cgtttgttaa agcctccatt gaagacgtgg tgaaaacgct gctggaagct atcgctctgg
    12661 ttttcctcgt tatgtatctg ttcctgcaaa acttccgcgc cacgctgata cccactatcg
    12721 ccgtgccggt ggtgttgatg ggaaccttct ccgtacttta cgccttcggt tacagcgtca
    12781 acaccttaac catgttcgcg atggtgctgg cgatcggtct gctggtggat gacgccatcg
    12841 tggtggtgga aaacgtcgaa cgtattatga gtgaggaagg actcactcct cgcgaagcca
    12901 cacgtaaatc gatggggcag atccaggggg cactggtcgg gattgcgatg gtgctttcgg
    12961 cggtatttgt accaatggcc ttcttcggcg gcaccaccgg tgccatctat cgccagttct
    13021 ctattaccat tgttgcggcg atggtgctgt cagtactggt agcgatgctc ctcactccgg
    13081 ctctgtgtgc cacgctactt aagccactga aaaaaggtga gcatcacggg caaaaaggtt
    13141 tttttgcctg gtttaaccag atgtttaacc gcaacgccga acgctacgaa aaaggggtgg
    13201 cgaaaattct ccaccgtagc ctgcgctgga ttgtgattta tgtcctgctg cttggcggca
    13261 tggtgttcct gttcctgcgt ttgccgacgt cgttcttacc gctggaagac cggggcatgt
    13321 ttactacctc ggtacaattg cccagcggtt caacccaaca acagaccctg aaagtcgttg
    13381 agcaaatcga gaaatattac ttcacccatg aaaaagacaa catcatgtcg gtgtttgcca
    13441 ccgttggttc tggccctggg ggtaacgggc aaaacgtggc gagaatgttt atccgcctga
    13501 aagactggag cgaacgcgac agtaagaccg gcacctcgtt tgccattatc gagcgtgcaa
    13561 cgaaggcgtt taaccaaatt aaagaagctc gcgttatcgc cagcagccca ccagcaatta
    13621 gcggtcttgg tagttctgca ggttttgata tggagttgca ggaccacgct ggagcgggtc
    13681 acgatgcgct gatggcagca cgtaatcagt tgctggcgct ggcggcggaa aacccggagc
    13741 taacccgtgt gcgccataac ggcctcgatg acagtccgca gttgcagatt gatatcgacc
    13801 agcgtaaagc tcaggcgctg ggcgttgata tcgacgatat taacgacaca ctgcaaaccg
    13861 cctggggttc gagctatgtg aatgacttta tggatcgcgg tcgcgtgaag aaagtctatg
    13921 tgcaggcagc tgcgccgtat cgcatgctgc cagatgacat caatctctgg tatgtccgaa
    13981 ataaagatgg cggcatggtg cccttctctg ctttcgcgac ctcacgctgg gaaacaggct
    14041 cgccgcgtct ggaacgctat aacggttatt ctgcggttga gattgttggg gaagccgcac
    14101 cgggggtcag taccggtacg gcgatggata ttatggaatc gttagtgaag cagctgccaa
    14161 acggctttgg tctggagtgg acggcgatgt cgtatcagga gcggctttcc ggcgcgcagg
    14221 ctccggcgct gtacgccatt tccttgctgg tggtattcct gtgtctggcg gcattgtatg
    14281 aaagctggtc ggtaccgttc tcggtaatgc tggtcgtgcc gctgggggta atcggcgcgc
    14341 tgctggcaac ctggatgcgc gggctggaaa acgacgttta cttccaggtg ggcctgttaa
    14401 cggtcattgg tttatcggcg aaaaacgcca tcctgatcgt cgagtttgct aacgagatga
    14461 accaaaaagg ccatgacctg tttgaagcga cgctccacgc ctgccgtcag cgtttacgcc
    14521 cgattctgat gacctcgctg gcatttatct tcggcgtatt gccaatggca accagcacgg
    14581 gtgccggttc cggtggtcag catgcggtgg gtactggcgt aatgggcggg atgatttcag
    14641 ctactattct ggctatttac ttcgtgccgc tgttctttgt gctggtgcgc cgccgcttcc
    14701 cgctgaagcc gcgcccggaa taagcaataa aaaaggcgac atgccaatgt gtcgcctttt
    14761 tcaactttcc gattaagaac ctgctcagcg ggttcttgct gtttatactt tgtctcagga
    14821 attacttacg aagcataact tcgataaagt ctttccagtt ccccagttca cgttcaatca
    14881 taacaacctc tcttataatt atgggtattc tacggaaaca atataccgtg gtgaagctaa
    14941 tttactcgat tgctgcgatg actacctccg ggggacaaac cttatgtaaa tactatggtc
    15001 ctgcggtgat aatttgtatg tgatacacag caacatttcg agatattcat acggcatcta
    15061 atactgattt aattctggtt aaaatacaga cagataacaa gatgaatatt cttaatgttt
    15121 acgttaa
//
LOCUS       DAZSIO010000092        15124 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZSIO010000092 DAZSIO010000000
VERSION     DAZSIO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.092"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(319..1350)
                     /gene="rsmC"
                     /locus_tag="ACHX5T_002601"
     CDS             complement(319..1350)
                     /gene="rsmC"
                     /locus_tag="ACHX5T_002601"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR8467751.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1453..1866
                     /gene="holD"
                     /locus_tag="ACHX5T_002602"
     CDS             1453..1866
                     /gene="holD"
                     /locus_tag="ACHX5T_002602"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR8467752.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1835..2281
                     /gene="rimI"
                     /locus_tag="ACHX5T_002603"
     CDS             1835..2281
                     /gene="rimI"
                     /locus_tag="ACHX5T_002603"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR8467753.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2296..2973
                     /gene="yjjG"
                     /locus_tag="ACHX5T_002604"
     CDS             2296..2973
                     /gene="yjjG"
                     /locus_tag="ACHX5T_002604"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR8467754.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3064..4653
                     /gene="prfC"
                     /locus_tag="ACHX5T_002605"
     CDS             3064..4653
                     /gene="prfC"
                     /locus_tag="ACHX5T_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR8467755.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            5046..5651
                     /gene="osmY"
                     /locus_tag="ACHX5T_002606"
     CDS             5046..5651
                     /gene="osmY"
                     /locus_tag="ACHX5T_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR8467756.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5768..5929
                     /locus_tag="ACHX5T_002607"
     CDS             5768..5929
                     /locus_tag="ACHX5T_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR8467757.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6051..7124
                     /locus_tag="ACHX5T_002608"
     CDS             6051..7124
                     /locus_tag="ACHX5T_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR8467758.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7121..7903
                     /locus_tag="ACHX5T_002609"
     CDS             7121..7903
                     /locus_tag="ACHX5T_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR8467759.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(8117..8980)
                     /locus_tag="ACHX5T_002610"
     CDS             complement(8117..8980)
                     /locus_tag="ACHX5T_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR8467760.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(8952..10493)
                     /locus_tag="ACHX5T_002611"
     CDS             complement(8952..10493)
                     /locus_tag="ACHX5T_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR8467761.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            10751..11530
                     /gene="deoC"
                     /locus_tag="ACHX5T_002612"
     CDS             10751..11530
                     /gene="deoC"
                     /locus_tag="ACHX5T_002612"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR8467762.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            11657..12979
                     /gene="deoA"
                     /locus_tag="ACHX5T_002613"
     CDS             11657..12979
                     /gene="deoA"
                     /locus_tag="ACHX5T_002613"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR8467763.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            13031..14254
                     /gene="deoB"
                     /locus_tag="ACHX5T_002614"
     CDS             13031..14254
                     /gene="deoB"
                     /locus_tag="ACHX5T_002614"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR8467764.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            14311..15030
                     /gene="deoD"
                     /locus_tag="ACHX5T_002615"
     CDS             14311..15030
                     /gene="deoD"
                     /locus_tag="ACHX5T_002615"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR8467765.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         3584 a         3927 c         4103 g         3510 t
ORIGIN      
        1 aacacctgaa gctagcgcgt ctaccaattc cgccaccttc gcataccatc aattcttaaa
       61 aagaattgct accacggagg cgcattctag tggttttcag cttttcgtca atagttaatt
      121 atcgcctgat gtgtaattgc tggaaaaatg tccatcagga aactagcgtg caggtttggt
      181 aggcatgcgg gggcagatgc cagatgcgac gctggcgcgt cttatctggc ctacgaaggg
      241 ctaacgtgca ggttttgtag gtcggataag gcgttcacgc cgcatccgac acggtatgcg
      301 gcgacagata tcagatactc aacctttctt cgcctggcgg gtcataatgg cgcgatatac
      361 cttgaagcgc ccggtttgcg cgataacttc atggaagcca aatgtctcat ccagcacgtc
      421 cgggtaaggc aggaaggcgt tcgctacaat tcgcagctcg ccgccgctat taagatgacg
      481 caccgcgcca cgaatcagcg tttgcgccgc atccaggctg gtttgcatcc catcgtggaa
      541 cggcgggttg gagatgatca tatcaaaaca acctttcacc tcggaaaaga cgttgctggc
      601 aaagacttca ccttcaacac cgttggctgc aagtgttgcg cggctggctt ctaccgccgg
      661 ggcagagaca tcgcacaagg tgagacgaat tttcggcgaa tggcgcgcaa aggcaactga
      721 aagtacaccc gcgccacagc cgacatccag cactttacct ttcgtgtgcg gagttaacgt
      781 cgagagcagc aactggctac cgacatccag accgtcgcgg ctaaacacgc caggcagcgt
      841 tttgaccgtc aggccatcga cgctgtattc gccccagaat ttatccgcat caaatactgg
      901 ctgtttttcc agacgaccaa aatagaggcc acagcgacga gcgctgtcga ctttattcaa
      961 cggcgcataa tctgccagca tctgctcagc gctgcgcacg ccgctgcggt tctcgccaac
     1021 gacaaaaata tctgtcccca ctggcagcag agaaagtaaa ttcatcaact ggaactgggc
     1081 ttccggtttg ttcttcggcc agtagtaaat cagcgtatcg caatctgcga catcattcgc
     1141 cgtggcgacc agactaaaac gggcgttatc ccccatctgg cagcttaaca cctgccaatg
     1201 gtggaattgc tgggtatgag cacggctggc cgcggtatct aaacgcgcgg gcaggtcatc
     1261 ctgtaagtct ccggcaaaca gaatacggct ttgttcgaaa tcatcactgt gacgcagcaa
     1321 gacttcactt gccggggtaa atgcagacat ggaatgctcc tcaattgata ctggcggcga
     1381 ttatagccat atgttggcgc ggtatcgacg aatttgctat atttgcgccc ctgacaacag
     1441 gagcgattcg ctatgacatc ccgacgagac tggcagttac agcaactggg cattacccag
     1501 tggtcgctgc gtcgccctgg cgcgttgcag ggggagattg ccattgcgag cccggcacac
     1561 gtccgtctgg tgatggtggc aaacgatctt cccgccctga ctgatccttt agtgagcgat
     1621 gttctgcgcg cattaaccgt cagccccgat caagtgctgc aactgacgcc agaaaaaatc
     1681 gcgatgctgc cgcaaggcag tcgctgcaac agctggcggt tgggtactga cgaaccgcta
     1741 tcattggaag gcgctcaggt ggcatcaccg gcgctcaccg aattacgggc aaacccaacg
     1801 gcacgcgccg cgttatggca acaaatttgc acatatgaac acgatttctt ccctcgaaac
     1861 gactgattta ccagcggctt accacattga acaacgcgcc cacgcctttc cgtggagtga
     1921 aaaaaccttt gccagcaacc agggcgagcg ttatctcaac tttcagttaa cgcaaaacgg
     1981 caaaatggcg gcgtttgcga ttacgcaagt ggtgctggat gaagctacat tgttcaatat
     2041 tgcggtcgat cccgactatc agcgtcaggg gctgggtagg gcgctgctgg aacatctgat
     2101 cgacgaactg gaaaaacgcg gcgtggcgac actatggctg gaagtccgtg cctcaaatgc
     2161 tgccgccatt gccctgtacg aaagtttagg ctttaacgag gcgacgattc gccgcaatta
     2221 ctaccccacc acggacggtc gcgaagacgc catcatcatg gcgttgccaa tcagtatgta
     2281 agacaaggtg gaataatgaa gtgggactgg attttctttg atgccgatga aacgctgttt
     2341 acctttgact cattcaccgg cctgcagcgg atgtttcttg attacagcgt cacctttacc
     2401 gctgaagatt ttcaggacta tcaggccgtt aacaagccac tgtgggtgga ttatcaaaac
     2461 ggcgcgatca cttcattaca gcttcagcac gggcgtttcg agagctgggc cgaacggctg
     2521 aaagttgaag caggcttgct taacgatgcc tttattaatg cgatggcgga aatctgtacg
     2581 ccgttgccgg gcgcggtttc tctgcttaac gccattcgta gcaacgctaa aatcggcatc
     2641 atcaccaacg gttttagcgc cttgcagcaa gtgcgtctgg aacggacggg cctgcgtgat
     2701 tacttcgatt tgctggtgat ttccgaagaa gttggcgttg ccaaaccgaa taagaaaatt
     2761 ttcgattatg cgctggaaca ggcgggcaat cctgaccgtt cacgcgtgct gatggttggc
     2821 gacactgccg agtccgatat tctcggtggc atcaacgccg ggcttgcgac ctgctggctg
     2881 aatgcgcaca atcgcgagca accagaaggc atcgcgccca cctggaccgt ttcttcgttg
     2941 cacgaactgg agcagctcct gtgtaaacac tgattgcctc ccccccgttg atgggtaaaa
     3001 tagccgcaat ttttcgtttt caacaagcgc ggcgcgatgc cgcttactca agaagaaaga
     3061 attatgacgt tgtctcctta tttgaaagag gtggcgaagc gccgcacttt tgccattatt
     3121 tctcacccgg acgccggtaa aactaccatc accgagaagg tgctgctgtt cggacaggcc
     3181 attcagaccg ccggtacagt aaaaggccgt ggttccaacc agcacgctaa gtcggactgg
     3241 atggagatgg aaaagcagcg tgggatctcc attactacgt ctgtgatgca gtttccgtat
     3301 cacgattgcc tggttaacct gctcgacacc ccggggcacg aagacttctc ggaagatacc
     3361 tatcgtaccc tgacggcggt ggactgctgc ctgatggtta tcgacgccgc aaaaggtgtt
     3421 gaagatcgta cccgtaagct gatggaagtt acccgtctgc gcgacacgcc gatcctcacc
     3481 tttatgaaca aacttgaccg tgatatccgc gacccgatgg aactgctcga tgaagttgag
     3541 aacgagctga aaattggctg cgcaccgatc acttggccaa ttggctgcgg caagctgttt
     3601 aaaggcgttt accaccttta taaagatgaa acctatctct atcagagcgg taaagggcac
     3661 accattcagg aagtccgcat tgttaaaggg ctgaataacc cggatctcga tgctgcggtt
     3721 ggtgaagatc tggcacagca gctgcgtgac gaactggaac tggttaaagg cgcgtctaac
     3781 gagttcgaca aagagctgtt ccttgcgggc gaaatcactc cggtattctt cggtactgcg
     3841 ctgggtaact tcggcgttga tcatatgctg aatggcctgg tggagtgggc gcctgcgccg
     3901 atgccgcgtc agaccgatac ccgtaccgta gaagcgagcg aagataaatt taccggcttc
     3961 gtatttaaaa ttcaggccaa catggatccg aaacaccgcg accgcgtggc gtttatgcgt
     4021 gtggtgtccg gtaaatatga aaaaggcatg aaactgcgcc aggtgcgcac tgcgaaagat
     4081 gtggtgatct ccgacgcgct gacctttatg gcgggtgacc gttcgcacgt tgaagaagcg
     4141 tatccgggcg atatcctcgg cctgcacaac cacggcacca ttcagatcgg cgacaccttt
     4201 acccagggtg agatgatgaa gttcaccggt attccgaact tcgcaccaga actgttccgt
     4261 cgtatccgcc tgaaagatcc gctgaagcaa aaacagctgc tcaaagggct ggtacagctt
     4321 tccgaagagg gcgcggtgca ggtgttccgt ccaatctcca acaacgatct gatcgttggt
     4381 gcggttggtg tgctgcagtt tgatgtggtg gtagcgcgcc tgaagagcga atacaacgtt
     4441 gaagcagtgt atgagtcagt caacgttgcc actgcccgct gggtagaatg tgcagacgcg
     4501 aagaaattcg aagagttcaa gcgtaagaac gaaagccaac tggcgcttga tggcggcgat
     4561 aacctcgctt acatcgctac cagcatggtc aacctgcgcc tggcacagga acgttatccg
     4621 gacgttcagt tccaccagac ccgcgagcat taattcttgc ctccagggcg cggtagccgc
     4681 tgcgccctgt caatttccct tccttattag ccgcttacgg aatgttctta aaacattcac
     4741 ttttgcttat gttttcgctg atatcccgag cggtttcaaa attgtgatct atatttaaca
     4801 aagtgatgac atttctgatg gcgttaaata ccgttcaatg cgtagatatt agtatctaaa
     4861 gccgtcgatt gtcattctac cgatattaat aactgattca gaggctgtaa tggtcgttat
     4921 tcatcaccca tcgcttttgt gatggcgacc attgacttct gtagagggtg aagtctctcc
     4981 ctattcagca atgcaacctc gtgttgctag gctcaaatta cgagcaaaca tacaggaata
     5041 aatcgatgac tatgacaaga ctgaagattt cgaaaactct gctggctgta atgttgacct
     5101 ctgccgtcgc gaccggctct gcctacgcgg aaaacaacgc gcagactacc aatgaaagcg
     5161 cagggcaaaa agtcgatagc tctatgaata aagtcggtaa tttcatggat gacagcgcca
     5221 tcaccgcgaa agtgaaggcg gcgctggtgg atcatgacaa catcaagagc accgatatct
     5281 ctgtcaaaac cgatcaaaaa gtcgtgaccc tgagcggttt cgttgaaagt caggcccagg
     5341 ccgaagaggc agtgaaagtg gcgaaaggcg ttgaaggcgt gacctctgtc agcgacaaac
     5401 tgcacgttcg cgacgctaaa gaaggctcgg tgaagggcta cgcgggtgac accgccacca
     5461 ccagtgaaat caaagccaaa ctgctggcgg acgatatcgt cccttcacgt aaagtgaaag
     5521 ttgaaaccac cgatggcgtg gttcagctct ccggtaccgt agattctcag gcacaaagtg
     5581 accgtgctga aagtatcgcc aaagcggtag atggtgtgaa aagcgttaaa aatgatctga
     5641 aaactaagta attcgtcctc ccgaactttg tcgggaggcg cgatgtgcac cacactaaaa
     5701 atatcgcatt gagtgatttt cacgctcata tcaagcggtg acattaacta tggtaaagga
     5761 gacgcttatg tttcgttggg gcatcatatt tctggttatc gcgttaatcg ccgccgcact
     5821 tgggtttggt ggtctggccg gtaccgctgc aggcgcagct aaaattgtct ttgtcgtcgg
     5881 gattattctg ttcctagtga gtttgttcat gggccgaaaa cgaccctaga tttcagacca
     5941 ttaagaaaat tttatccaaa gccagtccag cggactggct tttgcggttt tagcgaataa
     6001 taaattgcgt tactttgtct ttctttcaca acaaaaacag gaaggcagaa gtggggcagc
     6061 gaatacctgt aacgcttggt aatattgcgc cgttgtcgct aaggccgttc cagcctggac
     6121 gaatagctct ggtgtgcgaa ggcggcggac agcgtggaat tttcacggct ggcgtgctgg
     6181 atgagtttat gcgcgcgcag tttaatcctt tcgatcttta tctcggcaca tctgccgggg
     6241 cgcagaacct ctcggcgttt atctgcaatc agcccggtta cgcgcgcaaa gtcatcatgc
     6301 gctataccac aaaacgcgaa tttttcgatc cattgcgctt tgtccgtgga ggaaatctta
     6361 tcgatctcga ctggctggtg gaggccaccg caagccagat gccgttgcaa atggataccg
     6421 ccgcgcggtt gtttgacagc ggcaaatcgt tttatatgtg cgcctgtcgt caggatgact
     6481 acgcgccgaa ttacttttta ccaaccaaac aaaactggct ggatgtgatt cgcgcctcca
     6541 gtgcgatacc tggcttttat cgtagcggag tgtcgctgga aggcattaac tacctggatg
     6601 gcgggatcag tgatgcgatt ccggttaaag aggcggcaag gcagggcgct aaaacgttgg
     6661 tcgtcatccg cactgtgccg tcacaaatgt actacacgcc gcagtggttc aaacgcatgg
     6721 aacgctggct gggtgacagt agcctgcagc cgctggtcaa tctggtgcag catcatgaaa
     6781 ccagctatcg tgacattcag caatttattg agaaaccacc gggcaagctg cggatatccg
     6841 aaatttatcc gccgaagcca ttacatagta tcgcgcttgg cagtcggatt ccggcgctgc
     6901 gtgaagacta taaacttggg cgtttatgcg gtcgttattt cctcgccacg gttggcaagc
     6961 tattaactga aaaagcgccg cttacccgcc atctggtgcc agtggtgacg ccggaatcga
     7021 ttgtcattcc gcctgcgcca gtcgccaacg atacgctggt tgccgaagtg agcgacgctc
     7081 cgcaggcgaa cgacccgaca tttaacaatg aggatctggc ttgatttgcc gttttatcga
     7141 cactcactgc cattttgatt tcccgccttt tattggcgat gaagaggcca gcctgcaacg
     7201 cgcggcacaa gcgggcgtag gcaagatcat tgttccggcg acagaggcgg aaaattttgc
     7261 ccgtgtgcag gcgttagcgg aaaattatca accgctgtat gccgtattgg gcttgcatcc
     7321 tggtatgttg gaaaaacata gcgatgtgtc tcttgagcag ctacagcagg cgctggaaag
     7381 gcgtccggcg aaggtggtgg cggtggggga gatcggtctg gatctcttcg gcgacgatcc
     7441 gcaatttgag aggcagcagt ggttactcga cgaacaactg aaactggcga aacgctacga
     7501 tctgccggtg atcctgcatt cacggcgcac gcacgacaaa ctggcgatgc atcttaaacg
     7561 ccacgatttg ccgcgtgctg gtgttgtcca cggattttct ggcagccagc aacaggctga
     7621 acggtttgta cagctgggct acaaaatcgg cgtaggcggt actatcacct atccacgcgc
     7681 cagtaaaacc cgcgatgtca tcgcaaaatt accgctggca tcgttgttgc tggaaaccga
     7741 tgcgccggat atgccgctca acggttttca ggggcagcct aaccgcccgg aacaggctgc
     7801 ccgtgtgttt gccgtgcttt gcgagttacg cccggaaccc gcggatgaga ttgccgaagt
     7861 gttgcttaat aacacgtatg cggtgtttaa cgttcgtggg tagtttgccc ttcgtaggca
     7921 gcatccggca gtcggcgcat aatgcctgat gcgacgctta acgcgtctta tcatgcctac
     7981 aggtttgtgc cgaaccgtag gccggataag gcgttcacgc cgcatccggc agttggcgca
     8041 caatgcctga tgcgacgctt gacgcgtctt atcaggccta caagtctgtg ccgaaccgta
     8101 ggccgtatcc ggcatgtcac aaatagagcg ccggaaatat caaccggctc accccgcgca
     8161 cctttaatgc atcagccaac ggctcaacgt cttccggcgt ggcgctcgcc cagctttgcg
     8221 cctcgccata cacgccgtgg gcatgaaacg cgttcaggcg aaccggaaca tcaccaagtc
     8281 ccttgataaa cgccgccagt tcttcgatgt gttgcaaata atccacctgg cctgggatca
     8341 tcagcaaacg cagttccgcc agcttgccgc gctctgccag caaatagatg ctgcgcttaa
     8401 tctgttgatt atcgcgtccg gttaattgtt gatgacattc gctaccccat gctttgagat
     8461 cgagcattgc gccgtcacac accgggagta atttttccca gccggtttcg ctcaacatac
     8521 cgttactgtc caccagacag gtgagatggc gcagttgcgg atcgtttttg atagcagtaa
     8581 acagcgccac tacaaacggc agttgggtcg tggcttcgcc gccgcttacc gttatccctt
     8641 caataaacag caccgctttg cgcacatggc taagcacttc gtccacgctc atggattgcg
     8701 ccattggtgt ggcatgttgc ggacacatct tcaggcaggt atcacattgc tcgcagacca
     8761 cagcgttcca caccactttg ccgtcaacaa tctgcaacgc ctgatgcgga cactgtggca
     8821 cgcactcccc acagtcattg caacgtccca tcgtccacgg attgtgacag tttttgcagc
     8881 gcagattgca gccctgcaaa aacagagcca gacgactgcc tggcccgtca acgcaggaga
     8941 aggggataat cttactgact aaagcgcatc tgctgttcat ggcttatcac gcgcggctgg
     9001 cgttccagaa tacgagtgtt gcgtgcggct tcttcgccca gccaggtggt gttggtgcgt
     9061 gaaccttcgg cgcgatattt ttctaaatcc gacaaacgca ccatataacc ggtaacgcga
     9121 accagatcgt taccgctgac attggcggta aattcacgca ttccggcttt aaaggcaccg
     9181 aggcaaagct gtaccagtgc ctgcgggtta cgtttgatgg tttcgtcgag cgtcagaatg
     9241 tcgctgatgc cggaataata ataagcatga tgcggggcga cagtttgcag atgggtgatc
     9301 ggatctggct catcgccata cggcaaacgc gcgcccggcg tggtgccgat atcggaactg
     9361 atccccgact gtgcgtgtaa catggcgcgt ttttgccagc catatttcac gggggtattg
     9421 gcgacaaact ccgccagttg cgcgctgatg cgataaccca cttcatttgc ggcggcttct
     9481 ttaccgtagc gcgcggcaat cccttctttc tcacacagca agttaaccgc ttccgccagc
     9541 ccatacatgc caaacattgg cacaaaacgt tcagggttaa tcagcccttc tttcaccagg
     9601 aagctattct caaagaagtg tgattgttga tagaggaatt cacaccgcgc atcgatgatg
     9661 gcgatctgct gctggcagta gtgcggtaga gtgcgcgtaa agaagtcatc cagcgattcg
     9721 ctgcgctcgg caatggcttt caggttaagg cgtaccagcg tgctgccacc acccgccagc
     9781 ggcagtgagt tgtaacagct cacaatcccg tagccccctt ttgtgaaaat tttatcatgc
     9841 accggaccgt tggcgatgtg cggtttgcta cattcacaga tgttcttcgc cacttccagc
     9901 agcaggtcat caggggtgat ttcaggatcg tagataaagg tcaggttcgg tgaaacctgc
     9961 gcgtctgcac gtaagatcgc gcgggtaatg ggcgaatcag acgggccgat attggcgtgc
    10021 ataaaggcat ctggcagggt tctgtcgagg taacgccaga aacgttttat tcgaacatcg
    10081 atctcgtttt gtgttagaat tctaacatac ggttgcaaca acgcatccag ttgccccagg
    10141 tagaccggca tcgatgtgac cgacggtacg tggtggtaaa gaatggtcag cagagagagt
    10201 gcgtcatcaa gatctttcgc gccttccagc tccagccatt cggaaccgtt cgccagaaaa
    10261 cgagcgtaat cgggtaagac atagcgcggt ttgtacggcg catgaccttc aaacatatcg
    10321 cagattacac cttcatccag cgcgcggcgg gcttcggcag gaagctgtgg gtaaggcaga
    10381 ttgttttctg cttccagtgc cagaaaatgg cgcttctgct ccgggctaag cactgggctg
    10441 gtgacaattt gctggcaacg ttgttgcagt gcattttcat gagaagtggg catcttcttt
    10501 tccttttatg ccgaaggtga tgcgccattg taagaagttt cgtgatgttc actttgatcc
    10561 tgatgcgttt gccaccactg acgcattcat ttgaaagtga attatttgaa ccagatcgca
    10621 ttacagtgat gcaaacttgt aagtagattt ccttaattgt gatgtgtatc gaagtgtgtt
    10681 gcggagtaga tgttagaata ctaacaaact cgcaaggtga attttattgg cgacaagcca
    10741 ggagaatgaa atgactgatc tgaaagcaag cagcctgcgt gcactgaaat tgatggacct
    10801 gaccaccctg aatgacgacg acaccgacga gaaagtaatt gctctgtgtc atcaggccaa
    10861 aactccggtc ggcaataccg ccgctatctg tatctatcct cgttttatcc cgattgctcg
    10921 caaaactctg aaagagcagg gcaccccgga aatccgtatc gctacggtaa ccaacttccc
    10981 acacggtaac gacgacatcg aaatcgcgct ggcagaaacc cgtgcggcaa tcgcctacgg
    11041 tgctgatgaa gttgacgtgg tgttcccgta ccgcgcgctg atggcgggtg acgagcaggt
    11101 tggttttgac ctggtgaaag cctgtaaaga ggcttgcgcg gcagcgaatg tactgctgaa
    11161 agtgatcatc gaaaccggcg aactgaaaga cgaagcgctg atccgtaaag cgtctgaaat
    11221 ctccatcaaa gcgggtgcgg acttcatcaa aacctctacc ggtaaagtgg ctgtgaacgc
    11281 gacgccggaa agcgctcgca tcatgatgga agtgatccgt gatatgggcg tagaaaaaac
    11341 cgttggtttc aaaccggcgg gcggcgtgcg tactgcggaa gatgcgcaga aatatctcgc
    11401 catcgcagat gaactgttcg gtgctgactg ggctgatgcg cgtcactacc gctttggtgc
    11461 ttccagcctg ctggcaagcc tgctgaaagc gctgggccac ggcgacggta agagcgccag
    11521 cagctactaa gtaagatgct ttacgcctga tgcgctgcgc ttatcaggcc tacgagacgt
    11581 atctacccgt aggccggata aggcgtagac gcatccggca aaagccgcct catactcttt
    11641 tcctcgggag gttaccttgt ttctcgcaca agaaattatt cgtaaaaaac gtgatggtca
    11701 tgcgctgagc gatgaagaaa ttcgtttctt tatcaacggt attcgcgaca acactatttc
    11761 cgaagggcag attgccgccc tcgcgatgac cattttcttc cacgatatga caatgcctga
    11821 gcgtgtctcg ctgaccatgg cgatgcgaga ttcaggaacc gttctcgact ggaaaagcct
    11881 gcatctgaat ggcccgattg ttgataaaca ctcgaccggc ggcgtcggcg atgtcacttc
    11941 gctgatgttg gggccgatgg tcgcagcctg cggcggctat attccgatga tctccggtcg
    12001 cggcctcggt catactggcg gtacgctcga caaactggaa tccatccctg gcttcgacat
    12061 tttcccggat gacaaccgtt tccgcgaaat tattaaagac gtcggcgtgg cgattatcgg
    12121 tcagaccagt tcactggctc cggctgataa acgtttctac gcgacccgtg atattaccgc
    12181 aaccgtggac tccatcccgc tgatcactgc ctcgatcctg gcgaagaaac tggcggaagg
    12241 tctggacgcg ctggtgatgg acgtgaaagt gggtagcggc gcgtttatgc cgacctacga
    12301 actctctgaa gcccttgccg aagcgattgt tggcgtggct aacggcgctg gcgtgcgcac
    12361 caccgcgctg ctcaccgaca tgaatcaggt actggcctcc agtgcaggta acgcggttga
    12421 agttcgtgaa gcggtgcagt tcctgacggg tgagtatcgt aacccgcgtc tgtttgatgt
    12481 cacgatggcg ctgtgcgtgg agatgttgat ctccggcaaa ctggcgaaag atgacgccga
    12541 agcgcgcgcg aaattgcagg cggtgctgga caacggtaaa gcggcagaag tctttggtcg
    12601 tatggtagcg gcacaaaaag gcccgaccga cttcgttgag aactacgcga agtatctgcc
    12661 gacagcgatg ctgacgaaag cagtctatgc tgataccgaa ggttttgtca gtgaaatgga
    12721 tacccgcgcg ctggggatgg cagtagttgc aatgggcggc ggtcgtcgtc aggcatctga
    12781 caccatcgat tacagcgtcg gctttactga tatggcgcgt ctgggcgacc aggtagacgg
    12841 tcagcgtccg ctggcggtta tcctcgcgaa agacgaaaac agctggcagg aagcggcgaa
    12901 agcggtgaaa gcggcaatta aacttgccga taacgcaccg gaaagcacac caactgtcta
    12961 tcgccgtatc agcgaataac ggtatactga tctgatcatt taaatttgaa gcactgagta
    13021 cggagaacat atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac
    13081 agaagatgca gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc
    13141 ttgtgccaaa ggcgaagctg ataatggtcg taaagggccg ctcaatctgc caaatctgac
    13201 ccgtctgggg ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga
    13261 cggcaacgct gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga
    13321 taccccgtct ggtcactggg aaatcgccgg tgtcccggtt ctgtttgagt ggggatattt
    13381 ctccgatcac gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa
    13441 tctgccgggt tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg
    13501 cgaagagcac atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca
    13561 gattgcctgc catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc
    13621 ccgtgaagag ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat
    13681 cggtgacaaa gctggtaact tccagcgtac cggtaaccgt cacgacttgg ctgttgagcc
    13741 gccagcaccg accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt
    13801 cggtaaaatt gcagacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg
    13861 cttggacgcg ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat
    13921 cgtcttcacc aacttcgttg acttcgactc ttcctggggc caccgccgcg acgtcgccgg
    13981 ttatgctgcg ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg
    14041 cgatgacgac atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac
    14101 tgaccacacg cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc
    14161 actgggtcac cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac
    14221 ttctgatatg gaatatggca aagccatgtt ctgatggatt tgggcggagc gttgactccg
    14281 cctttgttat gtcacaaaaa ggataaaaca atggctaccc cacacattaa tgcagaaatg
    14341 ggcgatttcg ctgacgtagt tttgatgcca ggcgacccgc tgcgtgcgaa gtatattgct
    14401 gaaactttcc ttgaagatgc ccgtgaagtg aacaacgttc gcggtatgct gggcttcact
    14461 ggtacttaca aaggccgcaa aatttccgta atgggtcacg gtatgggtat cccgtcctgc
    14521 tccatctaca ccaaagaact gatcaccgat ttcggcgtga agaaaattat ccgcgttggt
    14581 tcctgtggcg cagttctgcc gcacgtaaaa ctgcgcgacg tcgttatcgg tatgggggcc
    14641 tgcaccgatt ccaaagttaa ccgcatccgt tttaaagacc atgactttgc cgctatcgct
    14701 gactttgaca tggtgcgtaa cgcagtagat gcagctaaag cactgggcgt tgatgctcgc
    14761 gtgggtaacc tgttctccgc tgacctgttc tactctccgg acggcgaaat gttcgacgtg
    14821 atggaaaaat acggcatcct cggcgtggaa atggaagcgg ctggtatcta cggcgtggct
    14881 gcagagtttg gcgcgaaagc cctgaccatc tgcaccgtgt ctgaccacat ccgcactcac
    14941 gagcagacca ctgccgctga gcgtcagacc accttcaacg acatgatcaa aatcgcactg
    15001 gaatccgttc tgctgggcga taaagagtaa ttgtgtttcg ctgaaaggcg attgtcgtgt
    15061 taagccggag tgggggactg ctccggcttt ttagtatcta ttcatcaata gcaactgatt
    15121 tata
//
LOCUS       DAZSIO010000093        15084 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZSIO010000093 DAZSIO010000000
VERSION     DAZSIO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.093"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..179
                     /gene="yjbD"
                     /locus_tag="ACHX5T_002616"
     CDS             <1..179
                     /gene="yjbD"
                     /locus_tag="ACHX5T_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR8467766.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(312..1184)
                     /gene="rluF"
                     /locus_tag="ACHX5T_002617"
     CDS             complement(312..1184)
                     /gene="rluF"
                     /locus_tag="ACHX5T_002617"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR8467767.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1532..2479
                     /locus_tag="ACHX5T_002618"
     CDS             1532..2479
                     /locus_tag="ACHX5T_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFR8467768.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2549..3355
                     /locus_tag="ACHX5T_002619"
     CDS             2549..3355
                     /locus_tag="ACHX5T_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR8467769.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3365..3772
                     /locus_tag="ACHX5T_002620"
     CDS             3365..3772
                     /locus_tag="ACHX5T_002620"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR8467770.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3772..4266
                     /locus_tag="ACHX5T_002621"
     CDS             3772..4266
                     /locus_tag="ACHX5T_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFR8467771.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4332..5129
                     /locus_tag="ACHX5T_002622"
     CDS             4332..5129
                     /locus_tag="ACHX5T_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR8467772.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            5140..5928
                     /locus_tag="ACHX5T_002623"
     CDS             5140..5928
                     /locus_tag="ACHX5T_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IID"
                     /protein_id="HFR8467773.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5980..7209
                     /gene="sorE"
                     /locus_tag="ACHX5T_002624"
     CDS             5980..7209
                     /gene="sorE"
                     /locus_tag="ACHX5T_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFR8467774.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7591..8280
                     /gene="pepE"
                     /locus_tag="ACHX5T_002625"
     CDS             7591..8280
                     /gene="pepE"
                     /locus_tag="ACHX5T_002625"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFR8467775.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8371..10002)
                     /locus_tag="ACHX5T_002626"
     CDS             complement(8371..10002)
                     /locus_tag="ACHX5T_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR8467776.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10222..13905)
                     /gene="metH"
                     /locus_tag="ACHX5T_002627"
     CDS             complement(10222..13905)
                     /gene="metH"
                     /locus_tag="ACHX5T_002627"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR8467777.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14105..14929
                     /gene="iclR"
                     /locus_tag="ACHX5T_002628"
     CDS             14105..14929
                     /gene="iclR"
                     /locus_tag="ACHX5T_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR8467778.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3580 a         3980 c         3940 g         3584 t
ORIGIN      
        1 gcgtactgac caactccgaa accaacagca ttaagaaaga gtacatcgat aaattaatgg
       61 tcgagcgtga ggctgaagcg aaaaaagccc gccaattgaa gaaaaagcag gcttataaac
      121 cggatcctga agcgtcattt agctggtcag caaatacgtc gacgcgcggc aggcgctaat
      181 gaacaatcaa cgcctgagat tttcactcgt ctgtgtggac acaatcttcc cctcacccta
      241 accctctccc cgaaggggcg aggggactgt ccgagctctt tttggacgtt ttcatcagtc
      301 tgaggcgtta atcagcgtcc cttcttttta cgccccggcg aggtaaaacg cttaccgttg
      361 gacgccgggc gaccgccttt ttccgccgtt ttttccatct taacgactgg acgtttgatg
      421 cccgctgttt tcggtttggc cttcgcttta ggttttgcct cggaagagga attttcgatg
      481 agcttaaaga ggtcgattaa ctcatcgtcg gttaaatcgc gccactcccc cagcggaatg
      541 ccgcttaagc tgacgttcat aatgcgcgta cgttccagct ttttcacttc atagccgaaa
      601 tgctcgcaca tgcgccggat ctgacggttc agcccctgca ctagggtaat gcggaagaca
      661 aacggcgctt cttttttgac tttgcacttt ttcgtcaccg tcccgaggat cggcaccccc
      721 gcccccatac cacgaataaa ttcatcggta atcggtttat cgaccgtcac cagatactct
      781 ttctcatgat cgttacccgc acggaggatc ttattcacca gatcgccgtg attggtgagg
      841 aaaatcagcc cctgggagtc tttatccagg cggccaatcg ggaacacgcg tttgctgtgg
      901 ttaacgaaat cgacaatgtt atcgcgctcg ccatcttcgg tggtgctgac aataccgacg
      961 ggcttgttca gggcgataag taccaaatct tcggcttccc gaggctcaat caactgaccg
     1021 tttactttca caatgtcgcc gggtttcacc tgatcgccaa tggtggctcg cttgccatta
     1081 aggaacacat tgccttgctc gatatagcga tccgcttcgc ggcgtgagca aattccgctt
     1141 tcgctgatgt atttatttaa acggactgat gagtcgggca gcataaattc tcctgtaaaa
     1201 cgcggaatat accgtacctc ttagcgggga aaaacagggt tatgcggcaa cgtcgaaatt
     1261 aaatgtgatc tgccacgcct tttatcacaa aagtgtctcc ggaagtacga tgcaaatttg
     1321 cctggcaaaa taaggataat caacatatta cgaatcgctg cacacctgtg ctgatgacta
     1381 ctacgcagag aaacaaatgt gcaagagaaa gcgctatttc tccccatgca aagagtgaaa
     1441 aatcgctttg aaaggccctc atgatggggt tatatcagat caagcggtga gaaagcgtat
     1501 ttgcacaaat gtgcagaacc aggaggcggc aatggaaaac agtgacgata tccgtttgat
     1561 tgtgaagatt gcccaactct attacgaaca ggatatgacg caggcgcaaa tcgcgcgcga
     1621 actgggtatt taccgcacca ccatcagccg cttgcttaaa cgaggccgcg atcagggaat
     1681 ggtcaccatc gccatcaact atgactacaa cgaaaatctc tggctggagc agcaactgaa
     1741 gcaaaagttt ggcctgaaag acgttgtggt ggtgtcggga aatgatgagg atgaagagac
     1801 tcaactggcg atgatggggt tacacggcgc gcaactgctg gatcgcttgc tggagcctgg
     1861 cgatattgtc ggtttttcct ggggtcgcgc ggtgagcgca ctggttgaaa acttgccgca
     1921 ggcggggcaa tcgcggcagt taatctgcgt accaattatt ggcggaccgt ccggtaaact
     1981 cgaaagccgc tatcatgtaa acacattaac ctacagcgcg gcagcgaagc tgaaagggga
     2041 atcgcatctc gcggattttc cggctctgct ggataaccca ttaattcgta atgggatcat
     2101 gcagtctcag cactttaaaa ccatctctgc ctactgggat aatctggata tcgccctggt
     2161 gggaattggc tcaccggcca ttcgcgacgg cgctaactgg catgcgtttt atggtggtga
     2221 agagagtgac gacctgaatg cccgccaggt tgctggcgat atttgctcgc gcttttttga
     2281 tattcacggc gcaatggttg aaacgaatat gagcgaaaaa acgctctcta tcgaaatgaa
     2341 taaattaaag caggcgcgat attccattgg cattgccatg agtgaagaaa aatacagcgg
     2401 aattattggt gcactgcgtg gaaaatatat taattgtctg gtaacgaata gcagcacagc
     2461 tgaactatta ctgaaataaa aatcttcccg atgtttaccg taataaaaat tacggcgggt
     2521 ccctattatc taaaatcagg agtctgttat gcaaacgtgg ttaaatttgc aggataaaat
     2581 cattattgtc accggcggcg catccggtat tggtctggcg attgtagagg aattattagc
     2641 acaaggcgcg aatgtacaga tggtcgatat tcacggtggc gatggtcaat atgaaggtca
     2701 taaaggctat cagttttggc cgaccgatat ttctagcaca aaagaggtaa atcatacggt
     2761 agcggaaatt atccagcgtt ttggtcgcat cgacggtctg gtcaataacg ccggggtcaa
     2821 tttcccgcgt ctgctggtcg atgagaaagc gcctgccggg cagtatgaac tcaacgaagc
     2881 tgcattcgaa aaaatggtca atatcaacca gaaaggtgtt tttctgatgt cgcaggcggt
     2941 ggcgcgacag atggtcaaac aacatgatgg cgtgattgtg aatgtttcct cagaaagtgg
     3001 gctggaaggc tcagaaggac aaagctgtta cgccgctacc aaagccgcgc tcaatagctt
     3061 cacgcgctcc tggtcgaaag agctgggtaa acacggtatc cgtgtggtcg gtatcgcgcc
     3121 cgggattctg gaaaaaaccg ggctacgcac gccggaatat gaagaggcgc tggcgtggac
     3181 gcgcaatatc accgtcgagc agctgcgtga aggctatacc aaaaacgcca ttcctattgg
     3241 gcgcgccgga agattagcag aagtagctga ttttgtttgt tatctgttgt ctgaacgcgc
     3301 cagctacatc accggagtaa ccactaacat tgcgggcggc aaaacgcgcg gctaaggagg
     3361 ctttatggtc aacgccattt tttgtgccca cggcaaatta gcttgcgcca tgctggaatc
     3421 ggttcagatg gtttacggca acgccaatgt tgaagccgtt gcatttgtgc ccggtgaaaa
     3481 cgccggagac attgcggtaa agctggaaaa gttagtaagc attcacaatc aagatgagtg
     3541 gctgattgcc gtggatttac agtgcggcag cccgtggaat gcggcagcaa tgcttgcgat
     3601 gcgcaatcct cgtttgcggg taatcagcgg cctttctctg ccgctggcgc tcgaactggt
     3661 tgataaccag gacagcatga atgttgatga actgtgtgaa cacctgacgc aaatcgcaaa
     3721 gcaaacctgc gttgtctgga aacaactggc aacgacagag gaggatttct gatgaacatt
     3781 acgctcgccc gcattgatga ccgtctgatc cacggccagg tcactaccgt ctggtcgaaa
     3841 gtggctaacg cccagcgcat tattatctgc aatgacgaag tttataacga tgaagtgcgc
     3901 cggacattat tgcgtcaggc tgcgccaccg ggaatgaagg ttaacgtggt caatattgaa
     3961 aaagccgtcg cggtttatca caacccgcaa tatcaggacg aaaccgtttt ttatcttttc
     4021 actcgcccac aggatgcctt agcgatggtt cgccaggggg taaaaatagg caccctgaat
     4081 attggcggca tggcctggcg gcccggtaaa aaacagttaa ccaaagcggt ttcattagat
     4141 gatgatgata ttaacgcatt tcatgagtta aataatcttg gtgtcatact ggatttacgc
     4201 gtcgtagcgt cagatccctc aatcaatatt atcgacaaaa taaacgaaca gttaatcgca
     4261 aattaaataa tagcgcctgt gtacatatgc tttaacaggc agggatgacg acacttaaaa
     4321 ggtgacaaat catggaaata agtaccctac aaatcattgc tatatttctt ttttcctgta
     4381 ttgccggaat gggcagcgtg ctggatgaat ttcagactca tcgcccgtta attgcctgta
     4441 cggtgattgg tttaattctc ggtgatttaa aaaccggaat aatactcggc ggtacgctgg
     4501 aattgatagc tctctgctgg atgaacgtcg gcgcggcgca atctccggat tctgcactcg
     4561 ccagcataat ctccgccatt ctggttatcg ttggtcagca aagcatcgcc accggaatcg
     4621 ccatcgcgtt gcctgtggct gcggcaggcc aggtgctgac ggtgtttgcc cgtaccatca
     4681 ccgtagtgtt ccagcacgcg gcggataaag cagcagaaga ggcgcggttt cgcactctcg
     4741 atattctgca tgtctccgcg cttggcgtgc aggcgctgcg cgtcgccatt ccggcactga
     4801 ttgtctcact gttcgtcagc gccgatatgg tcagcaatat gttaaacgcc attcccgaat
     4861 ttgtgacccg aggactacag attgctggcg gttttatcgt ggtggtcggt tacgccatgg
     4921 tgctacgtat gatgggcgtg aaatatttga tgcctttctt tttcctcggt ttcctcgcag
     4981 gtggctacct cgatctcagc ctgctggcgt tcggtggcgt cggcgtgatc atggccctgg
     5041 tctacatcca gctaaatcca cagtggtgta aagctgaacc tcaactccag gccaccgcct
     5101 ccaccgccct tgaccaactt gacgattaac ggagccatca tggaacagag aaaaattaca
     5161 cgcagcgatc tggtgagcat gtttctgcgc tccaacctgc aacaggcgtc ctttaacttt
     5221 gaacgtattc acgggctggg cttttgctac gacatgatcc ccgccatcaa gcgactttac
     5281 ccattaaaag aggatcaggt tgcggcgctc aggctacacc tggtgttctt caataccacg
     5341 ccagccgtat gtggcccggt catcggcgcg gaaattgatg acggtaccat caacggcatc
     5401 aaagtcggtc tgatgggacc attggcagga gttggcgatc cactggtctg gggaacgctg
     5461 cgcccgatta ccgccgcgct cggcgcatct ctggcacttt cgggcaacat tctcggcccg
     5521 ctgctgttct tctttatttt caacgcggtg cgtctggcga tgaagtggta tggcctacag
     5581 ctcggctttc gcaaaggggt gaatatcgtc agcgatatgg gcgggaatgt gctgcaaaaa
     5641 ctcaccgaag gcgcgtcgat tctcggactg tttgtgatgg gcgtgctggt gaccaaatgg
     5701 acgtcaatca acgtaccgtt ggtggtttca caaacgcatg ccgccgatgg ctccaccgtc
     5761 accatgaccg tgcagaacat tctcgaccaa ctttgccctg gtttgctggc gctcggtctg
     5821 acgctactaa tggttcgtct gctcaacaaa aaaattaacc cggtatggct gattttcgcc
     5881 ctgtttggct tagggattat cggcaatgct ctgggcttcc tgtcctgatt cttcgccccg
     5941 gcacgactgc cgaggccatc gctcaacatg aggtggttta tgaaaacaac agctctgcgt
     6001 ctttatggta aacgtgattt acgcctggaa acctttgacc ttcctgaaat gcaggaggat
     6061 gaaatcctcg caacggtggt cactgacagc ctgtgcctct cttcctggaa agaggccaat
     6121 ctgggtgaaa accataaaaa agtacccgac gatgtgacga ccaacccaat catcatcggc
     6181 cacgaatttt gcggcgatat tctggccgtg ggtaaaaagt ggcagcacaa attccagccg
     6241 ggtcagcgtt atgtgattca ggccaacctg caactccccg accgcccgga ttgccccggc
     6301 tactccttcc cgtgggtaga cggcgaagcg acgcatgtgg ttattcccaa cgaggtcatg
     6361 gaacaatatt gcctgctggc atacgacggc gaaacctatt ttgaaggctc actggttgaa
     6421 ccgctttcct gcgtgattgg cgcgttcaac gccaactatc atcttcagga aggtagttat
     6481 aaccacacga tggggattcg cccgcaaggg cacacgctga tcctcggcgg caccggacca
     6541 atgggactgt tggcgattga ttatgcgctg catggaccag ttaacccgtc gctgctcgtc
     6601 attaccgata ccgacaacga caaattgagt tatgcgcgca agcactatcc atcagaaccg
     6661 caaacactga ttcattatct caatgccacc gatgcagcat ttgatacgtt aatggcgctg
     6721 agtggcggtc acggcttcga tgatattttc gtctttgtgc ctaatgaagg actggtgact
     6781 ctcgcctctt ccttgctggc gacagatggt tgcctgaatt tcttcgccgg accgcaggat
     6841 aaacatttca gcgcgccaat taatttctac gatgtgcatt atgcatttac ccactacgtg
     6901 ggcacgtcag gcggcaatac cgacgacatg cgcgcggcgg tcaaactgat cgaagagaaa
     6961 aaagtgcagg ccgcaaaagt ggtaacacat attcttgggc tgaatgccgc gggcgaaacc
     7021 acgcttgaat tgcctgccgt cggcggcggc aaaaagctgg tgtataccgg gaaatacctg
     7081 ccgctgacgt cactcacgca gattcaggat caagcactgg cggcgattct ggcgcgtcat
     7141 caggggatct ggtccggtga ggtggagcaa tacctgctcg cccatgcaga ggcaatttcc
     7201 catgattaat cgcgctactt aactctgcat gtcgctggct ggtcgcccca gcaacttcgc
     7261 gaccggatgt gcggctgatt gctgaagcag cacaatttgc acgagaagga taaaaaatat
     7321 cagcgcctta aggggcgctc aaattggtgg caaagtacac attgtttatg ccggatgcgg
     7381 aatgaagcct tattcggtct acataatttt gaaaatccaa tatattgcag ttatctggta
     7441 ggccaggtaa gcgtagcgca tcaggcaatg ctgtgtttgt catcagtctc agcgcccggt
     7501 gcaatacgtg acatcgtgag ccatgtcagt aagtggttaa attcgcagtg gtagcatgcc
     7561 ttccgtgatt tcttactaac cggagaagta atggaactgc ttttattgag taactcgacg
     7621 ctgccgggta aagcctggct ggaacatgca ctgccgctaa ttgctgaaca gttgcagggt
     7681 cgccgctcag cggtgtttat ccctttcgct ggcgtaacgc agacctggga tgattacaca
     7741 gagaaaacgg ctgcggttct cgctccgctg ggtgtttctg tcaccggtat tcatagcgtt
     7801 gtcgatcccg ttgccgcgat tgaaaatgcg gaaattgtga ttgtcggcgg cggtaatact
     7861 ttccagttgc tgaaacagtg ccgcgagcgc gggctgctgg ctccaattac tgacgtggtt
     7921 aaacgtggcg cgctgtatat tggctggagc gcaggcgcta accttgcttg cccaaccatt
     7981 cgtaccacca acgatatgcc gattgtcgat ccgcaaggtt tcgatgcgct aaatctgttc
     8041 ccgttgcaaa tcaacccgca cttcaccaac gcgctgccgg aaggccataa aggtgaaacc
     8101 cgtgagcagc gtattcgcga actgctggtc gtcgcgccag aactgacgat tattggtctg
     8161 ccggaaggta actggatcac agtgagtaaa ggtcacgcta cgctgggtgg cccgaacacc
     8221 acttatgtgt ttaaggctgg tgaagaagcg gttccgctgg aagctggtca ccgtttttaa
     8281 gtgaattgcc ggatgcatga tccggcatct tgttcatagt gcctgatgcg acgcttgacg
     8341 cgtcttatca ggcctacagg attcacaata ttaatactcg tcccgtcctt catcttcatc
     8401 cggctgttcc agcacactgt aagccaccga acaaaacagc gagttcaggc gctgcatatc
     8461 tcccagtaag cctaaatgta gcgaactggt ttcaatgctc tgcacgtttt gctgatgcag
     8521 gcgatcgacg tgggcgtggg aatagcggcg attaagaatg cgaaaacgat gtttgctgcg
     8581 acgcaaacga cgagcgctgg tgacatcgcc agagaagaac accgacattg ccagctttaa
     8641 attactgagc aattgctcat agagcgcatc cagttccttc aacccatcaa gcgaaaatgc
     8701 ccgccgtgct gccagcgatt tatcggcaat ttcgctgccc atgcgctcga cgatatcgga
     8761 ggcctgttca aggttgagcg acatttcgat gatctccgcc cagcggcgcg actcttcctc
     8821 cgccaactct tcttttggca tccgcgccag atacagctta atggcggtgt agagaacgtt
     8881 gatatcatct gccagcttac gcagctcttt ctcctgccgt ggctcgccgt gcatcacttt
     8941 attcagccct tccatcatct gttccatggc gtcgccaatg cgcagggttt cgcgcgcggc
     9001 gttcgccaga gcaagcgtgg gcgtgtccag cgcgctgaca tccagatgtt taggccgtag
     9061 ctgggtatcc agttccggtt catcgcgaat aatcgttttg caaaaccgtg ccatcgggtc
     9121 aacaaatggc agcatgacca ggcaacgtac aaggttgtag aagacgtgga aatagatcac
     9181 cagttccgct tttggcaatg acagcttccc cattgtctct gccagcaaat ggacaaacgg
     9241 caggataatc aggctaccca ccagcttaaa cagcagacta cccagcgcga cacggcgggc
     9301 tgcggcattg gcggcactgt tgttgagcat cgccagcagg ccggaaccga ggttagctcc
     9361 aatcaccaga cagagcgcca cggggaagga gataatgcct gcagcggtca gagtcgcggt
     9421 cagcagtaca gcagcaaggc tggagtagct gataatggcg aacatcgcgc caatcagcgc
     9481 atccagcaga atatcgccgg tcagcgaggc aaagatcacc tgaacgccgt ttgcctgggt
     9541 gatcggcgtt acggcctgca caatcaactc cagcgccagc aaaatcagcc caagaccaat
     9601 accgacgcgg cccagttgcc cggcgcgtga ctgtttgcgt ccgaggaaaa aaatcacgcc
     9661 gataaaaata agtaacggtg acagccagga taagtcgaag gtgagaatac gcgccattag
     9721 cgccgtcccg acatctgccc ccagcacaat gaccagagcc ggtgcgaggg ctaccagatc
     9781 ctgagcgacg aacgaggtca ccagcatggt ggtggcatta ctgctctgta ccagtgcggt
     9841 aacgccgatc cccgcgcaaa aggcgagcgg ctttttttcg acgctccggc taaggacagt
     9901 acgcaaacgc gcgccgaaga cgcgcattac gccggttcga acaatatgag tcccccagac
     9961 cagcagggcg acggcagaaa gcaggtgaag cagcgttaac acgagttcag attctcctta
    10021 tcgttatatg ttcctgaagc aattgaatga ccaaaggacc ggaaacacgc tgactggcat
    10081 ctttactgtg ccttttgaca ttcgtttttc cggctaagat tattccagcc tgtttccagt
    10141 ataagtgttt catattaata aagagacagg gcactcattg agcgccctgt ttgttgtaag
    10201 gaaaagtgac agatttgtga atcagtccgc gtcatacccc agattcggtg ccagccagcg
    10261 ctcaacatcg gagacgctca tacctttacg gcgggcatgg tcttcaacct gatcgcgctg
    10321 aatttgcgcc accgcgtagt acttgctgtc cggatgactg aaataccaac ccgaaaccga
    10381 tgcaccaggc cacatcgcga aagattctgt gagtttcatg ccagtatgtt tttccacttc
    10441 cagcagctcc cagatggtgg ctttttccgt atgttccggg caggccggat agcccggtgc
    10501 cggacggatg ccctggtagt tttcgcggat cagttcttcg ttgctgaggt tctcgttcgg
    10561 cgcatagccc cagtagactt tacgcacacg ctcatggaga tactccgcaa aggcttcggc
    10621 taaacggtcg gcaagcgctt tcaccatgat tttgttgtaa tcatcgtgct gcgcttcaaa
    10681 ggcatcagcc agtgcgtcct cttccagccc gccagtcacg gcaaatgcgc cgatgtaatc
    10741 tgctttacca gaaagcttcg gcgcaacgaa gtcagcgaga cagtagttag cgaatcccgt
    10801 tttttcggtc tgttgacgca gatggtggct gacgttgatc acatgggtgc gcgtttcgtc
    10861 acggtagatt tcaatgtcat cgcccacacg gtttgccggg aacaggccca ccacgccacg
    10921 cggattcagc gttttctcgg cgcttaattt atccagcatg tcgttggcgt ctttaaacag
    10981 ccgctgcgcc tcaacgccca ccacttcatc ttccagaatg cgaggatact tcccggccag
    11041 cgaccaggtc ataaagaacg gtgtccagtc gatgtaatta cgcagcgttt cgatgctggc
    11101 ttccacttcc tgcacgccga gacggtgcgc caccggcggc gtgtaagcct gccagtcaaa
    11161 tgcgaaatcg ttatcgcgcg ccgcttccag cgtgaccggt ggtgtgcgtg gtttcttgcg
    11221 cccgtgctga atacgcacgg tttcgtactc cttgcaggta cgagcgacaa aatcatcacg
    11281 ctgagtatcg gaaagcagcg ccgctaccac accaacggtg cgcgaggcat tctgcacata
    11341 caccgtaggg ccgctgtagt tctgctcgat tttcaccgcc gtgtgcgctt ttgaggtggt
    11401 cgcgccgcca atcagtaacg gaatggcgaa gccctgacgc tccatctctt tcgccacgtt
    11461 aaccatctcg tccagcgacg gcgtgataag ccccgaaagg ccaatcaaat cagcattcac
    11521 ttctttggcg atacggagaa ttttttccgc aggcaccata acgccgagat cgacaatttc
    11581 gtagttgtta cattgcagca ccacaccaac gatattttta ccgatgtcgt ggacatcgcc
    11641 tttcacggtg gcgatcacca tcttgccgtt ggttttaccc tgctctttgc tggcttcaat
    11701 aaacggttcg agatacgcca ctgcctgttt catgacgcgc gccgatttga ccacctgtgg
    11761 caggaacatt ttgccttcgc caaacagatc gccgaccacg ttcatgccgt ccatcaacgg
    11821 gccttcaatc acttcaatcg ggcgcgtagc ctgctggcgg gcttcttcag tatcctgctc
    11881 gataaactcg gttatgcctt tgaccagcga gtattccaga cgtttattca cgtcccacga
    11941 gcgccactcc gcttgctggg cgttagcagt gtcgtccgct ttactgccgc gatatttctc
    12001 ggcaagctcc agtaaacgct cagtgccatc gtcacgacga ttaagaatca catcttccac
    12061 cgcgtcgcgc agttcagcgg gtaggtcgtc gtaaatcgcc agttgcccgg cgttgacgat
    12121 ccccatatcc atgccattgc gaatagcgta gtagaggaac actgcgtgaa tggcttcgcg
    12181 caccggatcg ttgccacgga atgagaaaga aacgttagat acgccgccgg aaatcagcgc
    12241 gtgcggcagt tcgcgtttga tgtcttcaca cgcgccgata aagtcctgcg cgtagttgtt
    12301 gtgctcttca ataccggtgg cgaccgcgaa gatgttcggg tcgaagatga tatcttctgg
    12361 cgggaagcca acctcttcgg tgaggatttt gtacgcccga cggcaaatct cgattttccg
    12421 tgcgcgagta tcggcctgtc cctgttcgtc aaaggccatc accaccactg ccgcaccgta
    12481 gcgacgcaac aatttcgcgt gatggataaa ggcgtcgacg ccctctttca tcgagataga
    12541 gttaacaatg cctttgccct ggatacactt cagacctttt tcaatgacat cccattttga
    12601 ggagtcgatc ataatcggca cgcgagcgat atccggttca ccggcaatca gattgagaaa
    12661 acgcaccatc gccgcttcgg catcgagcat cccttcatcc atgttgatat cgataatctg
    12721 cgcgccgttt tccacctgct gacgcgccac atccagcgcc tcgctgtatt tctcttcttt
    12781 gatcaggcgc ttgaacttag cagagccggt gacgttggtg cgttcaccca cgttcacaaa
    12841 caggctatcg tcgccaatgt tcagcggctc caggccagac aaacggcagg ctacgggaat
    12901 ttccggcagt ttgcgcggcg ctaatccttc tactgcacga ctcatcgctg caatatgttg
    12961 cggcgtggtc ccgcagcaac caccgacgat attgagaaag cccgcttgcg cccattcacg
    13021 tatctgtttt gccatcgtgt cggcgtcgag atcgtattca ccaaaggcgt taggcagccc
    13081 ggcgttcggg tgggcggtga cgtagcattc cgcaatccgt gatagttcct gcacgtactg
    13141 gcgcagttca tcgggcccca gcgcacagtt caggccaaag gtcagcgctt cggcgtggcg
    13201 caatgagttg taaaatgctt cggtggtctg cccggagagc gtgcgcccgg aggcgtcggt
    13261 gatggtgccg gagatcataa tcggcagctc aacgcccagc gcttcaaact ccgttttcac
    13321 cgcaaagacc gccgctttgg cgttaagggt gtcgaaaacg gtttcaatca ggatcagatc
    13381 cgcaccacct tccaccagcg ctttggtgga ttcgcgatag gccgccgcca gctggtcaaa
    13441 agtgatgttg cggaatgccg gatcgttgac gtccggagaa atagacgccg tgcggttggt
    13501 tgggccaaga acaccggcaa cgtagcgcgg tttctccggc gtgcgcgcgg tccactcgtc
    13561 agcacaggcg cgcgccagtt tcgccgccgc aaagttgatt tccgccgaca gggattccat
    13621 ctggtaatcc gccatcgcaa tggtcgtgga gttgaaggtg ttggtttcga tgatatccgc
    13681 gcccgcttca aagtaggcgt tgtggatagc ggcgatcact tccggtttac tgagtaccag
    13741 caggtcgttg ttgcctttga ggtcgcatgg ccagtcggca aagcgttcac cacgaaaatc
    13801 ggcttcgttc agtcgatagc tctggatcat ggtgcccata ccgccgtcca acaccagaat
    13861 acgttcattt aactgcgcac gcagttgttc cactttgctg ctcacacttg ctcccgacac
    13921 gcttaaccca gatttaataa aaattcaaca aaccatactg gaataaacgc atctgtggta
    13981 aaagcgacca ccacgcaaca tgagatttgt tcaacattaa ctcatcggat cagttcagca
    14041 actattgcat tagctaacaa taaaaatgaa aataatttcc acgatacaga aaaaggaggc
    14101 tgtcatggtc gcacccattc ccgcgaaacg cggcagaaaa cccgccgttg ccaccgcacc
    14161 agcgactgga caggttcagt ctttaacgcg tggcctgaaa ttactggagt ggattgccga
    14221 atccaatggc agcgtggcac tcacagagct ggcgcaacaa gccgggttac ccaattccac
    14281 gacccaccgc ctgctaacca cgatgcaaca gcagggtttc gtgcgtcagg tcggcgaact
    14341 gggacactgg gcaatcggcg cacatgcctt tatggtcggc agcagctttc tccagagccg
    14401 taatttgtta gcaattgttc accctatcct gcgcaatcta atggaagagt ctggcgaaac
    14461 ggtcaatatg gcggtgcttg atcaaagcga tcacgaagcg attattatcg accaggtaca
    14521 gtgtacccac ctgatgcgaa tgtccgcgcc tatcggcggt aaattgccga tgcacgcttc
    14581 cggtgcgggt aaagcctttt tagcccaact gagcgaagaa caggtgacga agctgctgca
    14641 ccgcaaaggg ttacatgcct atacccacgc aactctggtg tctcctgtgc atttaaaaga
    14701 agatctcgcc caaacgcgca aacggggtta ttcatttgac gatgaggaac atgcgctggg
    14761 gctacgttgc cttgcagcgt gtattttcga tgagcaccgc gaaccgtttg ccgcaatttc
    14821 tatttccgga ccgatttcac gtattaccga tgatcgcgtg accgagtttg gcgcgatggt
    14881 gattaaagcg gcgaaggaag tgacgctggc gtacggtgga atgcgctgac tttttctggc
    14941 gggcagaggc aatattctgc ccatcatacc tgagtggcaa tagaataagg gtgtctgtta
    15001 atcgcattga cgccaaaata acttaatgtc atacacttca cacactttta ttaactcagc
    15061 attattttta aacatcaaac cact
//
LOCUS       DAZSIO010000094        15037 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZSIO010000094 DAZSIO010000000
VERSION     DAZSIO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.094"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            163..648
                     /locus_tag="ACHX5T_002629"
     CDS             163..648
                     /locus_tag="ACHX5T_002629"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8467779.1"
                     /translation="MRAIYLSVQQAWNGKITYSVSGESEFAKKFQGKALPFDVRIISA
                     SQNEDWLVIATKVLPGADLRTYVDFKNSTVHVDSAGLEKVAKCINCNNTLQVNIPHEA
                     GHVLGYLDDDYDSSSPYVGDISGLMNVGMELWERYLKNATITLNIIMPETKFTLLNVT
                     K"
     gene            645..1031
                     /locus_tag="ACHX5T_002630"
     CDS             645..1031
                     /locus_tag="ACHX5T_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8467780.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1056..3269
                     /locus_tag="ACHX5T_002631"
     CDS             1056..3269
                     /locus_tag="ACHX5T_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFR8467781.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3292..3737
                     /locus_tag="ACHX5T_002632"
                     /pseudo
     CDS             3292..3737
                     /locus_tag="ACHX5T_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3759..7317
                     /locus_tag="ACHX5T_002633"
                     /pseudo
     CDS             3759..7317
                     /locus_tag="ACHX5T_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459289.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            7537..7740
                     /locus_tag="ACHX5T_002634"
     CDS             7537..7740
                     /locus_tag="ACHX5T_002634"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8467782.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7758..8078
                     /locus_tag="ACHX5T_002635"
     CDS             7758..8078
                     /locus_tag="ACHX5T_002635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8467783.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8144..8376)
                     /locus_tag="ACHX5T_002636"
                     /pseudo
     CDS             complement(8144..8376)
                     /locus_tag="ACHX5T_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8486..9256)
                     /gene="yafV"
                     /locus_tag="ACHX5T_002637"
     CDS             complement(8486..9256)
                     /gene="yafV"
                     /locus_tag="ACHX5T_002637"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR8467784.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9437..9883
                     /gene="ivy"
                     /locus_tag="ACHX5T_002638"
     CDS             9437..9883
                     /gene="ivy"
                     /locus_tag="ACHX5T_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR8467785.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9926..12370)
                     /gene="fadE"
                     /locus_tag="ACHX5T_002639"
     CDS             complement(9926..12370)
                     /gene="fadE"
                     /locus_tag="ACHX5T_002639"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR8467786.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12610..13188
                     /gene="lpcA"
                     /locus_tag="ACHX5T_002640"
     CDS             12610..13188
                     /gene="lpcA"
                     /locus_tag="ACHX5T_002640"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR8467787.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13394..14161
                     /locus_tag="ACHX5T_002641"
     CDS             13394..14161
                     /locus_tag="ACHX5T_002641"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR8467788.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14132..14872)
                     /gene="dpaA"
                     /locus_tag="ACHX5T_002642"
     CDS             complement(14132..14872)
                     /gene="dpaA"
                     /locus_tag="ACHX5T_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR8467789.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
BASE COUNT         3778 a         3719 c         4081 g         3459 t
ORIGIN      
        1 acaaatattg atggcaatat cattatacat aaggatggta ggaggacgta tttattaaat
       61 caaaatactg gttcgattac ggctgtgata agatggaagt atgagtagta taaggataat
      121 accgttacag atgactggac tatatgggaa aaagctattt ttatgcgagc gatatattta
      181 tctgttcagc aggcatggaa tgggaaaata acttattctg tttccggtga atctgagttc
      241 gctaaaaaat ttcagggtaa agctttgcct tttgatgtaa gaataatttc tgcatctcag
      301 aatgaagact ggttggttat tgcaacaaag gttttgcctg gtgctgattt aagaacatat
      361 gttgatttta aaaacagtac ggttcatgtt gattcagcgg gcctggaaaa agtagcgaaa
      421 tgtattaact gtaataatac gttgcaagta aatatacctc atgaagccgg acatgtactt
      481 ggttacttag atgatgatta tgattcttcg tctccgtatg ttggagatat tagtgggttg
      541 atgaatgtgg gaatggagct ttgggaacga taccttaaaa acgcaactat aacgttgaat
      601 atcataatgc ctgaaactaa atttacttta ttgaatgtaa ccaaatgaaa tttttctgta
      661 ttatagtgat tttcctttca ataaattaca tagctatagt gaatgttttc aagtcttctg
      721 cagttgctca atccaccaaa tattatgaag aaaatgatat acatgatgga agtaatgaca
      781 tcttttttat gaagaaatat gggccgcctg ctcatgagtt cgtgaggcca cgatataaat
      841 ttgttggtga gtattatatc ggcttgaatc aaacttatcg cataacagat cctgaatttc
      901 aagatgttcc tattaaggaa atgttctgga atttgcaggg ggatttgaat ctgacatgtt
      961 ggtttcatta taaagacggg caatggagag tgattactta tattttttgg ccaccagggg
     1021 ctagatttta atccggtatt aaaggagcaa atatcatgag ttttgtatct acgggtaata
     1081 aaccagttgg aaacggtgga cccgtcatca ccacaccacc aatagctggc gaaagtggcg
     1141 gtatgagcac aggttcagct gtaaccgatg tatctggtgc cgccgaggag atggcagaac
     1201 aggctgcggc ggacctgttt ggtgcgctgc cggaaccgtc aggtctggtt aaagccgcag
     1261 tagctgctgc gcaggctgcc gctgcagcag cagggatctc ggatatggct ggtgcagttc
     1321 aggatgcagc ggcgtcgctt gcagccggtg ctccgggggc gcataacgtc acagtaagtg
     1381 gtagtgcggt gccgccgcag atgcttttgt ttgcaggcat gaatggttcg gaagggcttg
     1441 ggaatctgtt ttcttacaca gtacagctta aaacgctgga cgccctgaac ctggggtacg
     1501 tttcccccgc tgctaacctg ccgcttcagc tcatggtggg caaagacctg tgcgtcagta
     1561 tcgaactgga cggcggcgga aaacggtata tcagcgggct ggtgacggcg gcgagggtgg
     1621 tcggccatga gagccgttcg gtaacgtatg aattacgcat tgagccatgg ctgaagctgc
     1681 tgacccatac cagcgactac aaagcgttcc agaacaaaac cgtggtggat attctcgatg
     1741 aagtactgga tgaatattcc tttccggtgg aaaagcggct ggtggaaaat tatccacccc
     1801 gcgcctggca ggtgcagtat ggtgaaaccg acttcgattt catccagcgc ctgatgcagg
     1861 aatgggggat ttactggtgg tttgagcaca gcgagaacag ccacacgctg gtgctggtgg
     1921 atgccatcaa tggtcataaa gcgtgccctg actcgccgct ggtggagtgg caccaggaag
     1981 ggctgaaact ggataaggaa tttatccaca ccattacggc cagtgagagg ctgcgtactg
     2041 gaaagtgggt gatggacgat tttgatttta tgaagcctcg ttcgctgctt aaaagtaccg
     2101 tggccagtcc gcgtgatacc ggtcatgctg aatacgagca ttacgaatgg cctggtgatt
     2161 acttcactac gggcgaaggc gagatgctga cgcgcattcg tatggaagcg cagcgcagcc
     2221 caggcagccg ggcacatggg gccggtcata tccgtaccct catgaccggg tacaccttta
     2281 cgttgatgaa tcacccgacg gcagaaatta atcaggaata tctgctggta cagaccactc
     2341 tgtttttacg ggataatgcg cagcacagcg gtcaggatca gcattttacc tatgtcacca
     2401 cgtttgaact gcaccccacc agcgaagttt accgcccaca acgaaccctg agcaaacccc
     2461 acaccaaagg cccgcagagc gccatcgtca ccggcccggc ggggcaggaa atctggacgg
     2521 ataaatatgg ccgggtgaag gtgcagtttg gctgggaccg ttacggaaag aatgacgaaa
     2581 acagttcctg ctggatccgc gtcagctatc cgtgggcggg taaaggattc ggcatgatcc
     2641 agatcccgcg tattggccag gaagtactgg tggatttcaa aaacggcgat ccggatttgc
     2701 ccatcatcgt ggggcgcacc tataaccagg acaccatgcc gccgtggggg ctgccgggca
     2761 tggcgtcgca gagtgggata ttcagccact cattgcaggg agggccgact aacggaaata
     2821 tgctgcgctt cgatgataaa acgggtgcgg aagaggtgaa gttccatgcg gaaaaagacc
     2881 tcaataccac ggtaaaaaac aatgaaaccc acacggtcat ggtcgatcgc ataaaaacca
     2941 ttgtcaaaaa tgaaaccatc agcgtgggag aagaccgtaa tgccaccatc acaaaaaatg
     3001 atggtctttc cgtcaaactg gctcaaacca tcaatgtcgg aacgacatat cgtctggatg
     3061 ttggcgatca atttaccctt cgttgcggca atgcagcgct tgtattacac aaggatggct
     3121 cgattgagtt ttgcggaaaa caactgttac tgcacaccag cgatgttatg caactcattg
     3181 gcagtggcat tgatatgaat ccggatggcg gcacagccat gactgcggat gatattgccc
     3241 cgttacctac ccccgacgtt tctgaataaa aaatataaaa ggaatttttc tatgaccagc
     3301 ctttaccgta ttcaggaggg ctgctttgcc ctgcctgaaa cttttcttga tcgcaccgtc
     3361 aatatcttcg tcccatcggg caatgaaagg gcaactccca gtctgaatat tttccgcgat
     3421 acgctcaggc cagatgaaaa tctgaccacc tatattgacc gccagatagc cttgatgaaa
     3481 aaaaaaacct aggccagcac cgggtacttt cgcggggaac ggcactggcg ggaactggcg
     3541 aagctgccat gacaggtgaa cagattctcg caactcatcg ttctggaaaa acagaagtgt
     3601 accagcgtca ggccgggttt atcaccgggc cagcaaaggt actaatgttg acgctgacca
     3661 ctcagcgtcc ttttgatgat cacaccgacc agctatggac tgcatggttg actagttttc
     3721 agcctgctaa aagctaaaaa aaacagggag gttttgtcat gtatgaagcc gcacgggtgg
     3781 atgaccccat ctaccacacc agcgcccttg ccgggttttt gataggggcc atcataggta
     3841 tcgccattat tgccgtcgcc agctttgctt tttttacctg cggctttttg gccgggctga
     3901 ttttgagttt tctggctgat cagatagctt ccggagtcct gcaactgggt gaggctattg
     3961 ggcgctcgat acattccaca gtgggaaaaa ttctgaccgg ttctgataat gtcagcacca
     4021 acagtcgccc ggccgcccgg gccgtactga gtacggtgat atgcgaagac cattccccgg
     4081 aaatccgcat tgcccaaggt tccggcaaca tctacatcaa cagccagctg cccgtaagga
     4141 tgaccacact gagtgtgatg cagtgattga ggacggctcc cccaatgtct ttctcggcgg
     4201 cggtacgcag acggtactgg aaatcagccc ggaaatccct gactggctgc gtcaggtggt
     4261 ggatgtgctg tatgtggtcg ccggaatgct tggcgggctg gctggcgcct ggcgtcaggc
     4321 ggcaaagatg gggtcgaaat ttggtacaaa atgtgcggca aaatttattg gtggcgaaat
     4381 ggtcgggatg gccatctctg acacagtcat gggattgttc agcaatccgg tagatgtcac
     4441 cactgggcaa aaaattttgc tgccggagac ggattttacc ctgccaggac gcctgcctgt
     4501 cacctgttcg cgcttttacg ccagccacct ggaaactgaa ggattgctgg gcagaggatg
     4561 gcggctgaac tgggagataa acctgcgtga ggatgaaaca tatatcacct tcatcggtgt
     4621 acagggtcgg gaactgagtt atccgaaaga gatgctgata ccgggccacc agatattcga
     4681 cccggaagag cagttttacc tgagccgtct gcatgacgga tgctatgtgc tgcattacac
     4741 cgactgcagc tattacgtgt ttgatgaatt tgatgaccac ggcgtcgcgc cgttactttt
     4801 tatggagacg ccgtaccggc agcgtattgc tttcgggcgt gagaacggca ggctggtgcg
     4861 ggtggcctcc agcagcggac accatctgtt attgcaccgc acgatgacgc aagcagggga
     4921 acgcctgtcg catattgaac ttttgaaggg cgggcgtccc ggtaacctgg tggaatatcg
     4981 ttacgacgat aacgggcaac tgactggcgt ggtgaaccgg gctggtgtga cggtgcgcca
     5041 gtttgcctat gaaaatggcc tgatgacaga gcatcgcaac gcgacgggct ttacctgtac
     5101 ctaccactgg gaagagattg aaggtttccc gcgcgtggtg gaacacacga ccagcgacgg
     5161 cgaggattac cggttccact atgattttgc tggcgggcag acggtggtaa ccggcaggcc
     5221 ggagcagaag tggcagtggt ggtttgatga agagacgtat gtgactgcgc accggacgcc
     5281 gggcggcgga atgtaccgct tcacgtacaa cgaaaaccat tttccggtag cggtggaact
     5341 ccccggcgaa cgcagggtaa cgctggaata cgacaccctg agccgggtgg tgaaagagac
     5401 ggacccggca ggccgggtga cacagacgca gtggaacggc agttttgctg aaatcacgcg
     5461 ccgggcgctg gatgacgacc acgtatggaa ggcggattac aacgaacacg gccaggtgat
     5521 acgggagacg gacccggaag ggcgcgtcac gcggtacggg tatgatgatc aggggctgcc
     5581 ggagacggtc tgccaccccg gtaaacagca ggaccgttac acctggaacg cgctggggtt
     5641 actgagcagc caccgtcgca tcacgggcag tgtgcagtcc tggcagtata cgcagcgcgg
     5701 gatgctggcg cgccatacgg atgaagagaa acgggagacg cgctggcagt acaccccgga
     5761 agggctggtt gccagtctga gcaacggcaa cggggctcag taccggttca gctacgacgg
     5821 cgacggacgg ctgacgggag aacagcgccc ggacggcctg ataaggatgt ttgcgctgaa
     5881 tgcggacggg tttccggtca tcatcccgac gcagggcaca gagggcgggg tgcgcaatga
     5941 acagcaggag cgtgatgcgc tgggccgcct gctgcgcagt gacacgcagc acagtacacg
     6001 gaccttcagc tacaaccgtc ttgaccagat aacggaagtg acgctcaccc ccacggaaga
     6061 aggggaacgg ctgcaccaca tgcaggcgga cacggtgcgt tttgcgtatg accgcagcgg
     6121 gtggctgacg gcagagcaca gcgtgcacgg cagtatcaaa taccggcgcg atgcgctggg
     6181 caatcccacc gatatcacgt tgccggacgg gcagcatctc agtcatcttt actacggcag
     6241 cgggcatctg ctccagacgg cactggacgg tatcacggtc agtgaatatg agcgtgacag
     6301 cctgcaccgt caggtgatac gcacgcaggg gaaactggcg acgttcagcg gctacaacgc
     6361 cgacaaccgg ttaagctggc agcgcagcct gccaggtggc agccagaatc cacagcaggc
     6421 ggtactcccc cggcgcagaa cgacagcgtg acggcgcggg actatatctg gaacgcagac
     6481 ggtgaagtcg gcggcatcaa tgacaaactg cgcggttgcc tggtgttcag ctatgaccgc
     6541 agcggctggc tgaccagccg cacggggcag atgtatgacc atgaccatta ttattacgac
     6601 aaagcgggca acctgctgac ggatgagtat cagggagcgg tgatggataa ccgcctgccg
     6661 ggttacgggc gtgaccgcta ccgctataac gaatggggcg aactgacaga gcgcagggac
     6721 cagcagctgg agtggaacgc gcagggccag ttaacgcggg tcatcagcag caacagcgag
     6781 acgcggtatc agtacgatgc gctgggcagg cgcatcagca aggcgaccag caatctccat
     6841 accgaccggg gagaacgaag ccggacaacg tttgtgtggg aagggttccg gctgttacag
     6901 gagacaacgt ggcagggaaa gcggacatac ctgtacgatg cggaacagcc gtacacgccg
     6961 gtggctgcca tcacggggcg cggagagagc cagaaaatct ggtattacca cacggacctg
     7021 acgggcacgg tacacgaggt aaccgcgccg gacgggacgc tggtgtgggc gggctaccag
     7081 gcgggatttg gtgaaaaccg gggcgacatc agcaacagcg gggcgtactt tgagcagccg
     7141 cagcggctgc cggggcagta ctttgacgaa gaaacagggc tgcattacaa tctgttcagg
     7201 tattatgcgc cggagtgcgg gaggttcgta agccaggacc cgataggatt aaacggtggg
     7261 ttgaacttat atgcttatgc gcctaatccg ttaggttgga tcgatccgct aggactgaat
     7321 tctttaggaa tagattcaaa taatatattt agaggagatg gtaactataa aggtggcgga
     7381 attggacagc caaagagtgg cacaatatca gcacaggaca ttattgatca tctctaagaa
     7441 aaaggtgatg ggcgtttaac atcttttagt actaaaaaaa catctagttc caactctcgt
     7501 ggtgcagatt ttttctcaaa aaaacatgtc ttcaaagtgg cagtttctga tctcgatgat
     7561 ttagttaaaa ataatcagat cactgtttta acacaagaaa aagtaaaagc aatatttggt
     7621 ttagaatcaa gcaagaaaat tagaaaagaa cttggtaata ttcttcagaa tatggctagg
     7681 aataatgagg tgttagttca tgggcaaatt cttggaaata aagttagccg atgtaattag
     7741 ttagtggaga ggaagttgtg gacgaaatta gaataagaat tgctggtaat acggctgcgc
     7801 cttgtttcca tgtgctaaaa aataagggat ataaagtaac aatggaaact catgcattat
     7861 ctgacgattg tttagattgt gtatatgatt ggtatgcgga aaaaaatgga caatttttta
     7921 gtgcaacgag tccagaagag ttacttggat tagttgcaat gtgggaagtt cgtggtaatg
     7981 actggagagc taatttgaat gagataaata cttatcaaaa agccatagaa gaatccccgt
     8041 tttttgataa aaacggcaat gaaatagagt ctgactaaat tttattgata tagcaacgat
     8101 aataaccgga aatttcttcc agatctttcc ggctttttat tatttaactt tcgttcagca
     8161 cactaaacat cccgtctcgc atccccttaa ctggctgttt aacctgataa agttccctgc
     8221 gcagttcctg tacttcgttg ttatcccaag caggatcaaa catccctccg agatcttcac
     8281 ggtaacgccc gtgccgatct caaatcccgc ttttctcagc catttggcgc tgattttgat
     8341 caccggcgtg tcgctgcggt tgggcagata acccacggta cagcgacgct gtgttttgga
     8401 aatggtgact tctggcgtaa tattgtgttt aagttagggg ctttcgtaat ctagcccagc
     8461 ccccggccct aaacacaacc cccactcacc acaacctaaa ctcatccgca tcccgccatg
     8521 ccggaaactt ttctctatat tcccgcaatg ccaccatcga cagctccgca tcaatacgcg
     8581 ttgccttatg cgcgtcggcg gtagcgataa tctcgccttg cggattaatc acccggctgt
     8641 caccgcgata atggcagccg ttgccatcgc tgccgacgcg attgcatccc gccacatacg
     8701 cctgattctc aatcgcgcgg gccgtcagca atgcctgcca gtgcagagag cgcggagctg
     8761 gccagttggc gacgtacagg gcgaggtcat aatcgttgag attgcgcgac cacactggaa
     8821 aacgtaagtc gtagcacacc agcggcaaaa tacgccagcc gcgccattcc acaatcactc
     8881 gcgcattgcc cgctttataa tgcagatgct catctgccat gcggaacaga tgacgcttat
     8941 cataaaaatg taccgtgccg cccggctcaa ccagcaaaaa gcggttaacc gaaccagact
     9001 ccgtttgtaa tgcaacactg cctgcaatca gcgcattgca ctgctgcgcc ttagctgtca
     9061 tccagttcac tacgtcatct tgtgcaagcg acgaagctgc cgcttccatg gcaaagccgc
     9121 tggtaaacat ctccggtaga acgatcacat cgcgcccggt aataccttcc agttgacgat
     9181 caaaatggcg caggttggca ggaccatcca cccacaccaa cggttgctgc aaaagcgtaa
     9241 tcttcaaact aggcacggtg tacaactcct ttatgcgaag ggttttataa ctttaacacc
     9301 ttatcaggca gttgccttag cgcagaataa attgataaca aatgctgata ttggaaatat
     9361 ctgatttgca aattatcgtg ttatcgctag gctttaggag gttaataaca tgggcaggat
     9421 aagctcggga ggaatgatgt ttaaggcaat aacgacagtc gccgcactgg tcatcgccac
     9481 cagtgcaatg gcgcaggatg atttaaccat tagcagcctt gcaaagggcg aaaccaccaa
     9541 agctgcgttt aatcagatgg tgcaagggca taagctacct gcatgggtga tgaaaggcgg
     9601 tacttatact cccgcacaaa ccgtaacgtt gggagatgag acgtatcagg tgatgagcgc
     9661 gtgcaaaccg catgactgtg gctcgcaacg tatcgctgtg atgtggtccg agaaatctaa
     9721 tcagatgacg gggctgttct cggctattga tgagaaaacg tcgcaagaga aactcacctg
     9781 gctgaatgtg aacgatgcgc tttcgattga tggtaaaacg gtgctgttcg cggcgttgac
     9841 cggcagcctg gaaaaccatc cggatggctt taattttaaa taattagcgg ataaagaaac
     9901 ggagcctttc ggctccgtta ttcaattacg cggcttcaac tttccgcact ttctccggca
     9961 actttaccgg cttcgtcgcc agctcttccg gatcaaagtc atcaacgtta atactgcgca
    10021 gacggctttc ttccgctttc accagaatag cggcttcatc tttatcaatc agccccttcg
    10081 tcagcgcgtt gtgcgccagt tcatccagac gggtaaatgg cagattttta cccagctctt
    10141 tacagatccg ctgatgaatc gggtcggcgg caatcacatc caccaacgcc tcttccagta
    10201 aaccaaccgg gttatgctcg cttggcgtca ggtactgacc gcgaccaata cgtgaacgag
    10261 tggcgttcgg cacttgtaaa atcttcgcca ttttatgatc cagcttgtca gacggtgcca
    10321 gataatgacg tccggtcggg aagatcacca cattcagcag cccggcaacc acgcggttcg
    10381 ggaagttttg cagtaaattg tccattgcct gttctgcctg atacagcgca tcctgtacgc
    10441 cccagtgcac cagcggcaga tcggcttcat tacggccttc gtcgtcataa cgcttcagaa
    10501 cggcagaggc gaggtaaagc tggcttaaaa tatcccccag acgtgccgag atacgctcac
    10561 gacgtttcag gctgccgccc agcagtgcca tcgagacatc agaaagcagg gcgaggttgg
    10621 cgctcaggcg gttcaggtgc tgatagtagc gtttagtggc atcgccggtt ggcgtgctgc
    10681 tggttaaacc gcgcgtcagg cccagccaga agctgcgaac tttgttgcta ccgacgtgac
    10741 cgatatgttt gaacaacagt ttatcgaacg cgttgacgtc attgttcttc gccgcttcca
    10801 tctcttccag cacgtacgga tggcaacgaa tcgctccttg tccgaagatc atcatgctgc
    10861 gggtcagaat gttagcccct tcaacggtga tggcaatcgg tgcgccctgg taagcacgcg
    10921 ccaggaagtt gctttgcccg agcataatgc ctttaccgcc ggtaatatcc atcgcatcaa
    10981 taatcgactg ctgcccgcgg tgggtacagt gatacttaac gatagccgac agcacggcag
    11041 gtttttcgcc gagcataatg ccgtaggtaa tcagcgatgc cgcagcatcc attacataag
    11101 cgttcccggc aatacgcgcc agcggctctt caatcccttc catcttaccg atagagattt
    11161 tgaactgacg gcgaatgtga gaatacgcgc ccgttgccag cgctaccgat ttcacgccgc
    11221 cggttgagtt ggaaggcagg gtgatgccgc ggcctaccga gaggcactcc accagcatcc
    11281 gccagccttg cccggccatt ttcggcccgc cgatgatgta atcgatcggc acgaagacat
    11341 ctttaccgcg cgtcggtccg ttctggaacg gtacgttcag cgggaagtgg cgacgaccaa
    11401 tttccacgcc cggcgtggtg gttgggatca gcgcacaggt aatgcctaaa tcttctgcac
    11461 cgccgagtaa tttttccggg tcggagagtt taaacgccag cccaagcacg gtcgcaatcg
    11521 gtgccagcgt aatgtagcgt ttgttccagg tcagacgcat ccccagcacc tgctggccct
    11581 gccattcgcc catgcagaca atcccggtgt ccggaatcgc gcccgcatcg gaacccgctt
    11641 ccgggctggt cagtgcaaag caggggatct cctgaccacg cgccagacgc ggcagatagt
    11701 gatctttctg ctcgtcagtg ccgtaatgtt gcaacagttc gcccgggcct aatgagtttg
    11761 gcacgccgac ggtaatcgcc aggatcccgc tcacgccaga gagtttttgc agcacacgag
    11821 actgggcata agctgagaac tccagtccgc cgtactcttt tttgataatc atcgcgaaga
    11881 agcgatgctc tttaaggtac gcccacaact ccggtggcag atccgccagc tcatgagtaa
    11941 tctggaaatc attcgccatc cggcaggctt cttctaccgg gccgtcgaga aacgcttgct
    12001 cttcagcggt caggcgcggc tgcggatagt tatgcagctt tttccagtcc ggcttgccct
    12061 ggaacaagtc gccctcccac caggtggtgc ccgcatcaat cgcttctttc tcagtgcgcg
    12121 acatcggcgg catcacctta cggaaaccgc gaaataccgg cgcggaaatc atcgacttac
    12181 gcataggcgc aaagttaaat ggcacgagga taatggtcag aggcaccagt acccacgccg
    12241 accacagacc agcaacgccg agggcggctg tccaggcgag caaaatcaga ctgctgataa
    12301 ataagctcac gcggtgatag aacaacgcgc cgagcaggac aaccgtagcg agaatactca
    12361 aaatcatcat aacgaaaagc cccttacttg taggaggtct gaccacttgt gatgatatgg
    12421 ttgtagtgga tgtaaaaaca tttagcaatg tgtttacaat ataattacaa caaagctcac
    12481 attgttgctg tttttatccg cacttcaggt caaaaagtcc tggtcatagc acctgcccgt
    12541 acttctcgct tttggcggta tccggtacac tgcattttgt ctattacatt tatgctgaag
    12601 gatatcctca tgtaccagga tcttattcgt aacgaactga acgaagcggc ggaaacgctg
    12661 gctaactttt taaaagatga cgccaatatt cacgccattc agcgcgcggc ggtcctgtta
    12721 gcagacagct ttaaagccgg tggcaaagtg ctttcctgcg gcaacggcgg ttcccattgc
    12781 gacgctatgc actttgccga agagttgacc ggtcgctacc gtgaaaaccg tccgggctac
    12841 ccggcgattg ctatttctga cgttagtcat atttcctgcg tcggtaatga tttcggtttc
    12901 aatgatattt tctcccgcta cgttgaagcg gtaggtcgcg aaggcgatgt actgctgggg
    12961 atctccacct ccggtaactc tgcaaacgtg atcaaagcga tcgcagcggc gcgtgagaag
    13021 gggatgaaag tgatcaccct gaccggtaaa gacggcggta aaatggctgg cacggcggat
    13081 atcgaaattc gcgttccgca ctttggttat gccgaccgca ttcaggagat tcacattaaa
    13141 gtgatccata tcctgatcca gttaattgaa aaagagatgg ttaagtaagt ctggcgtagg
    13201 ccggataagg cgtttacgcc gcatccggca cttgtgcgct gatgcctgat gcgatgctga
    13261 cgcgtcttat catgcctaca aatctgtacc cgaaccgtag gccgaataat tcgtttacgc
    13321 cgcatccgac ctgaaaattc ttaaatcaat cttcgccggg gtccatgcgc tcccgctgtt
    13381 gtggaggtta cccatgtgcg aattgctcgg gatgagcgcc aacgtcccta ccgatatctg
    13441 ctttagtttc accggacttg tacagcgtgg tggtggaacc gggccacata aagatggctg
    13501 gggcattacc ttttacgaag gtaaaggctg tcgcacattt aaagatccac aacccagctt
    13561 taattccccc atcgccaaac ttgtccagga ctacccgata aaatcctgtt cggtggtggc
    13621 tcatattcgc caggctaatc ggggcgaggt ggcgctggaa aatactcacc catttacccg
    13681 cgagttatgg gggcgtaact ggacttatgc ccataacgga caactgacgg gctacaaatc
    13741 actggaaacc ggcaacttcc gcccggtcgg tgaaaccgac agcgaaaaag ccttttgctg
    13801 gctgctgcat aaattaacgc agcgttaccc gcgcacgccg ggcaacatgg cggcagtgtt
    13861 taaatatatc gcctcactgg cggatgaact gcgggcgaag ggcgttttca acatgctgct
    13921 ttcggacggg cgctatgtaa tggcgtattg ctcgactaat ttacactgga tcacccgccg
    13981 cgcgccgttt ggcgtggcaa cgttgctgga tcaggatgtg gaaatcgact tcagctcgca
    14041 gaccacaccg aatgatgtgg tcacggtgat tgcgacacag ccgctgacgg gcaatgaaac
    14101 ctggcaaaaa attatgccag gcgaatggcg cttattttgc ctcggggagc gtgtagtttg
    14161 atgccagttg tggctgcacc acttcgtggc ttaacggctt gctgaccacg taacggccat
    14221 tggccacaga aacggtcggt ggcttacggg tttgctcaaa gtagtcgtag cccggcttca
    14281 gctgctccca gaagtcctta aagttggaat atttatggcg cttcatattg gcgtcggtca
    14341 tgcggaatgg gtaaatactc acctgcacgc tcggctgacc aaacaccagc gcaccagtaa
    14401 cgaactggaa gatctcatca ataccctgat tggtcattgc gtagcagccg atggatacac
    14461 aatcgccgtg gatcatcagg tatttccctt cataaccatg cgcacggtca taagcattgg
    14521 ggaatccaat attaatcgct ttgtagtaac ggctgtctgg ttttaactga ttacgctgga
    14581 cgctataaaa cccttccggg cttttgaaat cgccctgacg ctgttttggc cctaagccgc
    14641 cggaatattt acagatttta taactgtcga gcagttgata ttgctcgccc attttgacgt
    14701 aaagatcgag cgtacgttct tccttgaaga tctggatgta gacaggggat cccatcaact
    14761 gctgcttata ctctttgctc actggcgtgg tcgagctact gctgcccagc aaaccggcaa
    14821 acgaaacgca cgggatcaac aacatcgcaa gaattaatgc gattttacgc atactgctta
    14881 ttccttgata aaacggttac acacgccagg acggcaaaat ggatcccaaa tcggaatagt
    14941 ctggatttgg aaggctcaca ttatcaccaa aagagtttta cgcaagcctg tcggcgctgg
    15001 gtttacaaat ttagcacagg ggcagttttc aatagtc
//
LOCUS       DAZSIO010000095        14899 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZSIO010000095 DAZSIO010000000
VERSION     DAZSIO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.095"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            93..335
                     /gene="yjbE"
                     /locus_tag="ACHX5T_002643"
     CDS             93..335
                     /gene="yjbE"
                     /locus_tag="ACHX5T_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR8467790.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            449..1087
                     /locus_tag="ACHX5T_002644"
     CDS             449..1087
                     /locus_tag="ACHX5T_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR8467791.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1084..1821
                     /locus_tag="ACHX5T_002645"
     CDS             1084..1821
                     /locus_tag="ACHX5T_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR8467792.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1821..3917
                     /locus_tag="ACHX5T_002646"
     CDS             1821..3917
                     /locus_tag="ACHX5T_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR8467793.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3964..4242)
                     /locus_tag="ACHX5T_002647"
     CDS             complement(3964..4242)
                     /locus_tag="ACHX5T_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8467794.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4243..4386
                     /locus_tag="ACHX5T_002648"
     CDS             4243..4386
                     /locus_tag="ACHX5T_002648"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8467795.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4512..4922
                     /gene="psiE"
                     /locus_tag="ACHX5T_002649"
     CDS             4512..4922
                     /gene="psiE"
                     /locus_tag="ACHX5T_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR8467796.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4966..6441)
                     /gene="xylE"
                     /locus_tag="ACHX5T_002650"
     CDS             complement(4966..6441)
                     /gene="xylE"
                     /locus_tag="ACHX5T_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR8467797.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6812..7702)
                     /gene="malG"
                     /locus_tag="ACHX5T_002651"
     CDS             complement(6812..7702)
                     /gene="malG"
                     /locus_tag="ACHX5T_002651"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR8467798.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7717..9261)
                     /gene="malF"
                     /locus_tag="ACHX5T_002652"
     CDS             complement(7717..9261)
                     /gene="malF"
                     /locus_tag="ACHX5T_002652"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFR8467799.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9415..10605)
                     /gene="malE"
                     /locus_tag="ACHX5T_002653"
     CDS             complement(9415..10605)
                     /gene="malE"
                     /locus_tag="ACHX5T_002653"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR8467800.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10970..12085
                     /gene="malK"
                     /locus_tag="ACHX5T_002654"
     CDS             10970..12085
                     /gene="malK"
                     /locus_tag="ACHX5T_002654"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR8467801.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12157..13497
                     /gene="lamB"
                     /locus_tag="ACHX5T_002655"
     CDS             12157..13497
                     /gene="lamB"
                     /locus_tag="ACHX5T_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR8467802.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13649..14569
                     /gene="malM"
                     /locus_tag="ACHX5T_002656"
     CDS             13649..14569
                     /gene="malM"
                     /locus_tag="ACHX5T_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR8467803.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14798..>14899
                     /locus_tag="ACHX5T_002657"
     CDS             14798..>14899
                     /locus_tag="ACHX5T_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8467804.1"
                     /translation="MIGHITNSGGNNSLMKHDVVQGNNIVDLDLLRNF"
BASE COUNT         3694 a         3829 c         3908 g         3468 t
ORIGIN      
        1 cgccgtaatt cgcatgcttt agttgtgtat actcgatccc gcccgaaatg tttttgggta
       61 aatctccatt cattcaatga agggaaattg ttatgaaaaa agttctgtat ggcatttttg
      121 ccatatctgc gcttgcggcg acttctgcgt gggctgcacc tgtacaggtg ggcgaagcgg
      181 cagagtcggc agcaacgtcg gtttcggcgg ggagttcctc cgcgaccagc gtcagcaccg
      241 taagctcggc ggtgggtgtc gcgctcgcgg caaccggtgg cggtgatggt tctaataccg
      301 ggaccaccac aaccacgacc accagtaccc agtaataaag tatgtatccc caaaataatt
      361 tgagtcattg catctgtggc ttgaagtatg aagggattta ccataaccac actccggtgt
      421 ggttattctg cccctctgga gaagagtcgt gaagcgacct gcactcattc ttatctgcct
      481 gctattacag gcctgttcag ccacgactaa agagctgggc aattcactgt gggacagtct
      541 gttcggcacg ccaggcgtac agctgacgga cgatgatatt caaaatatgc cctacgccag
      601 ccagtacatg cagcttaatg gcgggccgca attgtttgtg gtgctggcct tcgctgaaga
      661 cggacaacaa aaatgggtca ctcaggatca agccactctt gtcacacaac atggtcgtct
      721 ggtgaagact ttgcttggcg gcgacaacct gattgaagtg aataaccttg ccgccgaccc
      781 gctgattaaa cccgcacaaa ttgttgatgg tgcaacatgg acgcgcacga tgggctggac
      841 cgagtaccag caggtacgct acgccaccgc acgctcagtc ttcaaatggg atggcaccga
      901 taccgtcaag gtcggcagcg atgaaaccct ggttcgcgtg ctggacgaag aagtctccac
      961 cgaccaggcg cgctggcata accgctactg gatcgacagc gaagggcaaa ttcgccagtc
     1021 ggaacagtat ctcggcgcgg attatttccc ggtgaaaacc actctcatca aggcggcaaa
     1081 acaatgatta aacaaactat tgtcgcgttg cttttgagtg tgggagcgtc atcggtcttc
     1141 gcggcaggaa ccgtcaaggt gttcagcaat ggcagcagtg aggccaaaac gctaacgggc
     1201 gcagagcatt taatcgatct ggtagggcaa cctcgactgg caaacagctg gtggcctggt
     1261 gcggtgatta gcgaagagct ggcaacggcg gcagcattgc gtcagcagca ggcgttgctg
     1321 acacggctgg cagaacaggg cgcagattcc agcaccgacg atgccgccgc cattaacgcc
     1381 ttacgccagc aaatccaggc attgaaagtg acaggcaggc aaaaaatcaa tcttgatccc
     1441 gatatcgtcc gcgttgccga acgcggtaac ccgccgttgc agggcaacta cacgctgtgg
     1501 gtcggaccac cgccgtccac ggtcacgttg ttcgggctta tcagccatcc tggcaatcag
     1561 ccattcactc caggtcgcga cgtggcgagc tatctctctg accagagcct gctcagcggt
     1621 gcggatcgca gctacgcgtg ggtcgtttac ccggacggac gcacgcaaaa agcgccggtg
     1681 gcttactgga acaagcgtca cgtagagccg atgcccggca gcattattta tgttggcctc
     1741 gcagattccg tctggagtga gacgcctgat gcccttaacg ccgacattct tcagactctg
     1801 acgcagcgga taccccaata atgaaaaaaa gacatctgct tagcttactg gcgctgggca
     1861 ttagcacagc ttgctacggc gaaacatatc ctgcgcccat tggtccgtcg cagtcggatt
     1921 tcggtggcgt aggattatta caaactccca ccgcgcgtat ggcacgggaa ggggagttga
     1981 gtctgaacta tcgcgataac gatcagtacc gttattactc agcttcagtg caactcttcc
     2041 cgtggctgga aacaacgctg cgctacaccg acgtgcgcac ccggcagtac agcagcgtcg
     2101 aagcgttctc tggcgatcaa acgtataaag ataaagcctt cgatctcaaa ctgcgtttgt
     2161 gggaagagag ttactggctg ccgcaagtgg cggttggtgc gcgggatatc ggcggtacgg
     2221 ggctgtttga tgcggaatat cttgttgcca gcaaagcctg ggggccgttc gattttacgc
     2281 tcggcctggg ctggggatat ctgggcacca gcggtaatgt aaaaaatccg ctctgttcag
     2341 ccagtgataa atattgctat cgcgataaca gctacaaaca ggcgggatct atcgacggta
     2401 gccagatgtt ccacggtcct gcctcactgt ttggcggcgt ggaataccag acgccctggc
     2461 aaccgctgcg tctgaaactg gagtatgaag gtaataatta tcagcaggat tttgccggga
     2521 agctggagca aaaaagtaag tttaacgtcg gggcgattta tcgcgttacc gattgggccg
     2581 acgttaacct tagctatgaa cgtggcaaca cctttatgtt tggcgtcacg ctgcgcacca
     2641 actttaacga tctgcgcccg tcctacatcg ataacgcccg cccgcaatat caaccgcagc
     2701 cgcaggatgc cattttgcag cattcggtgg tggcgaatca gttaacgttg ctgaaataca
     2761 acgccggact tgccgatccg cagatccagg cgaaaggcga tacgctgtat gttaccggcg
     2821 agcaggtgaa atatcgtgat tcgcgcgaag ggatcatccg tgctaatcgg atcgtgatga
     2881 acgatctgcc ggatgggatc aaaacgatcc gcattacgga aaatcgcctt aacatgccgc
     2941 aagtgacgac ggaaaccgat gtcgccagcc tgaaaaatca tctcgccgga gagccgttgg
     3001 gccacgaaac gacgctggcg cacaaacgcg tcgagccagt ggttccgaag tccaccgagc
     3061 agggctggta tatcgacaaa tcacgctttg atttccatat cgatccggtg ctgaaccagt
     3121 cggtcggtgg cccggaaaac ttttacatgt atcagctggg cgtgatggga acggcagatt
     3181 tgtggctgac ggaccatcta ctgaccaccg gcagcctgtt tgcaaatctt gccaacaact
     3241 acgacaagtt taactacacc aatccaccgc aggactcgca cttaccgcgc gttcgtaccc
     3301 atgtgcgcga gtatgtgcag aacgatgtct atgtgaataa cctgcaagcc aactacttcc
     3361 agcatctggg caacggcttc tacggtcagg tctatggagg ttatctcgaa accatgtttg
     3421 gcggtgcggg ggccgaagtg ttgtatcgtc cgctggacag taactgggcg tttggtctgg
     3481 atgccaacta cgttaaacag cgcgactggc gtagtgcaaa agatatgatg aaattcaccg
     3541 actacagcgt gaaaaccgga catctgaccg cctactggac gccatctttc gcccaggacg
     3601 tgttggtgaa agccagcgtc gggcagtatc tggcagggga taaaggcggc acgctggaga
     3661 tcgctaaacg ctttgacagc ggcgtggtgg tgggtggcta tgccacgatc accaacgttt
     3721 cgaaagagga gtacggcgaa ggggacttca ccaaaggcgt gtatgtctcg gtaccgttgg
     3781 atctcttctc gtctggcccg acacgcagcc gtgcggcgat tggctggacg ccgctgacgc
     3841 gtgacggtgg tcagcaactt gggcgtaagt tccagttgta tgacatgacc agcgaccgta
     3901 gcgtaaattt ccgctaagtc atgggaaagg tgccagtttt cgcactcact ggcaccctca
     3961 ttcttaatga cctgttgagc catcaggaac gcagttcacc cccggtttac gatctggtgt
     4021 gtaaaggcga aaacattctg tcttcctgtc cgtcgatatc ggttgcaggt ctggttgcct
     4081 gagcgactgg gccatcattg ggctggagac attgctttgc ggtaggtcgg ctttatcagc
     4141 agtaccgagc ggaccagcat aagcaggaag aacagagact gataacatca aagcagcaaa
     4201 ataaggcttc atttttacca cctttatcag gttacgtttc atttgttcca gaggaacatt
     4261 gtcgattttt cgcgcattgc tggtggctgg gaatcacctg aatgggtgat ttttgaatta
     4321 ccggctttgg tgcggtttgt cttgccggat gcgccgccag gcgcggctta tccggcctac
     4381 gggtaggtat atccggcttt ggtggaggcg cgctccaaat ccaggttgaa caaaacatac
     4441 acaaaaaata tagatctccg tcacattttt gcgttataca ggaagctcgc cactgtgaag
     4501 gaggtactgc tatgacgtca ctctctcgtc cgcgcgtgga gtttatctcc accattttgc
     4561 agaccgtact caatcttggc ctgctgtgcc tcggcctgat tttggttgtc ttcctcggca
     4621 aagaaacggt gcatctggct gatgtgctgt tcgcgccaga acaaaccagc aaatatgagc
     4681 tggtagaagg actggtggtt tactttctct atttcgaatt tatcgcgctg attgtgaagt
     4741 actttcagtc cggttttcac ttcccgctac gttactttgt ctatattggg atcaccgcca
     4801 ttgtgcgctt gatcatcgtc gatcataaat cgccactgga tgtgttgatc tactcggctg
     4861 cgatcctgtt actggtgatc accctgtggc tgtgtaattc gaaacggctg aagcgggagt
     4921 aaaaagacag cacgccgaaa tggcgcggcg tgctggacag gaagattaca gcgtagcagt
     4981 ttgttgtgtt ttcttcgttt ccggttccca gagcgcttcc agctcctcaa gggttttacc
     5041 tttggtttcc gggacaaatt tccacataaa cagtgctgcc agaacgccca tacaaccgta
     5101 aatccagtag gagaaaccgt tgtggaaatg ggccaccagc caggagtttt tgtccatcat
     5161 cgggaaggtc caggagacga agtagttcgc cagccactgg gccgccaccg cgattgccag
     5221 cgctttacca cgaatagcat tcgggaagat ttccgacagc agtacccagc ataccggacc
     5281 ccaggacatg gcaaaggcgg caacatagaa cagcatcgac agtagcgcca caatacccga
     5341 tgcctgagtg taaaacgcgg taccgaggct aaacataccg attgccattc cgagtgcgcc
     5401 gataatttgc agtggcttac gaccaaattt atccacagtc ataattgcca gtacggtaaa
     5461 ggtgaggttg ataactccga caataatggt ctgcaacagc gcgatatccg tgctggcccc
     5521 cagcgttttg aacacttccg gcgcgtagta cagcaccaca ttgatgccga caaattgctg
     5581 gaagatggag agcataacgc cgattacaat cacgcccacg ccaaacatca gcagacgacc
     5641 accggttttg cggccatgat ccagggagtg tttaatttcc tgtactgcct gagttgcaag
     5701 cgtgttgccc ataattttgc gcaggatact ttccgtctgt tcttgcttgc cgcgtgacat
     5761 cagccagcga ggactttctg gcacggtata cagcagcatt aagaacagca gtgcagggat
     5821 acattccgag gcaaacatat aacgcctgcc gtcagtattc agccagctgg catcaccgga
     5881 acgggcaata aaatagttta cgcagtaaac taaaagttgc ccgaaaataa tcgcaaactg
     5941 gttaaatgaa accagttttc cgcgaatatg agctggagcc agttccgcaa tatacattgg
     6001 tgaaagcatt gaggctaaac caacgccaat accgccaata atacgataaa taacaaattc
     6061 cgggacataa cctgccagat aaataggtac tgtgttgtcc gggtttatag aggtaaaacc
     6121 aagttctggc caggcagaac ctacaccaga aataaaaaac aggacagcag aaatcttaag
     6181 tgaatcacga cggccgaagc ggttactgca ataaccaccg agggcaccgc cgatgatgca
     6241 accaatcaga gcgctggcta cgcaaaatcc taacagggag ttggcagcgg attcacttaa
     6301 gttttgtgga gcaacaaaga cggtattgag tgactcaaca gtaccggaaa taacggcggt
     6361 gtcgtagcca aataataaac cacctaatgt agcgactaag gtaatcgaaa atatataact
     6421 ggaattatac tgggtattca ttcagacctg ccttagacca ttctgatctt ttatcagtgc
     6481 attgatgttg gaattgtcag gcagttaagg caattatcat tttttacaat gcgtttacgt
     6541 aatgtctttt ctgtgatctt aattgtgata attatcaaaa attgatgaaa caatactatt
     6601 gccgtgactc agagcacgaa agagaattat cgtaagtggg aaaacaaata acgtaaaaat
     6661 aataagttct atgatggcaa tattaaccgg cgaacgattc agattgcaga cgaaagaaaa
     6721 aaaggcgctc cgtggagcgc cgaataatag tcacaagttg ggataacgta agttgagggt
     6781 gcagcggcat aacattggca gaacaacatc tttaaccttt cacaccacct gccgtcaggc
     6841 cgttgaccag ccagcgttga gccagcaaga agacgatggt gatcggtaat gcagacatca
     6901 cggcagcggc ggcaaagtca ccccacaggt agttttgcgg gttgaggtat tgctgcatcc
     6961 ccacggccag ggtgtagctg tttacgtcac gcagtaacag cgacgcgacc ggaacttcag
     7021 taatggcagc gataaacgac aggatgaata ccaccgccag aatcggtact gacagcggca
     7081 acaggacaag gcggaaggcc tgccacggtg tcgcaccatc cagcgcagcc gcttcttcca
     7141 gcgaactgtc gatggtttcg aaatagcctt tgatggtcca gacatgcagc gcaatcccac
     7201 ccagatacgc gaaaattacg ccgccgtgag tattcaggcc aatgaatgga atgtactcac
     7261 ccagacgatc aaacaacgca tacaacgcga ccagtgaaag tactgccggg aacatctgga
     7321 aaatcagcat tcctttcagc agcgtcgctt tgcctggaaa gcgcatacgg gcgaaagcgt
     7381 aggcgcaggt ggtggagagc gccacaatgc caatcgcgga aatcccagcg acctttaccg
     7441 agttccacag ccacagcagt accgggaatg gcggtggcgt aatgcgacca tcagcctgtt
     7501 caacgctaaa acctaacgcc agtttccagt gatcccagga gatttgctcc gggatcaggc
     7561 taccggtcgc gaagtttccc tgacgcagcg agatagcgac gaccatcagc agcgggaaca
     7621 taatcgccgc gataaaaagt agcagtagca ggtgagtaat aaacaaacgt gctttttgcg
     7681 atttcggttg gaccattgcc atttttgtta tctcccttaa tcaaacttca ttcgcgtggc
     7741 tttcaggttc actatcgcca gcgcacccac cagcaggaag atcagcgtgg caattgctgc
     7801 cgccagaccg aagtcctgac ccccgccgcc ttcaaaagcg atacggtagg tgtagttaac
     7861 aagcaggtcg gtataaccgg ctggcgtggt cgtgccaaga cgatccgggc cgccgttggt
     7921 taacagctga atcagcacga agttgttaaa gttaaaggcg aagctggcga tcatcagcgg
     7981 cgtcagcggt ttaatcagca gcggcagcgt aatcttaaag aagttctgga acggacctgc
     8041 gccatccatt gctgaggctt catacaaatc gtccggaatc gctttcagca agcccatgca
     8101 gaggatcatc atgtacggat aacccagcca ggtattgacg ataatcagca tcgtgcgagc
     8161 ggtggtcgga tcgctgaacc aggcgggctt cacgccaaac agcgcgctca acatcatgtt
     8221 gatttcaccg aagctctggt taaacaatcc tttgaaaatc aagattgaaa tgaacgatgg
     8281 caccgcgtag ggcagaatca gcaggacgcg atagaccgct ttgccgcgca acgcttccca
     8341 ctgcaccaga catgccagaa ccatgccgac cgccaccgtt aaaaagacag tgatcagcga
     8401 gaacaccacg gtccagacga aaatggcgag gaacggtttc tgaatgcctt cgtcggtaaa
     8461 gacgcgggta aagtttttcc agccggtggt cacggtgtaa ccggggctta gcttttcatc
     8521 cccccagttg ccgtcggcgg taatggactg gtaaaagcca atttggttat tcggacgata
     8581 tttcacgcca ctctgattat tcgtcaacgt gccgtcaccg tcgagtgtgt agagcggctg
     8641 cgtgccagaa aactggcgca gggagctcat catcactttg ttgccatccg gcagaatggc
     8701 ggtaatgtca ctcagcgcct gacgattctg ggtaatcacg cgcagattcg cgcgttcgcc
     8761 ttcgggctgg gcggtcgttt ctttcagttg cagtttttgc tcgccgccaa atttaaacgc
     8821 gtcggagagg taatttttgc cggtttcgcc gtcgctgagc gccagttgcc actcatcgcc
     8881 cgccgggtaa agaccaaagt tataggtttt gcctgcttgc caggagcgat ctaacaacac
     8941 ttcctgcgca cgttcaaaag tcagctggtt agtgctgctg tagttggtga aggcaatggc
     9001 gatggtgcag accagaggga agaggacgaa taatcccatt ccagccattc ccgggtaaac
     9061 atagcgccag gcgtaggctt tacgattggc gaaaatatac agccccgctg aactcaatat
     9121 cagcgtggta atggcgaaca ggtattcccc ttgtgcgtac attaaaacaa caaggtaacc
     9181 caccagcagg ccgagcagac ctagcactga ccatttcagc gcgtcgcttt gccaccaatg
     9241 tttcttttta atgacatcca tggggttctt cctcattcca ggacggataa ggctttcacg
     9301 ccttatccga caacaactgc ctgatgcgac actgatgcgt cttatcaggc ctacatgcgt
     9361 ttcagttttg taggccggac aaggcgttca cgccgcatcc ggcatttcac agcattactt
     9421 ggtgatacga gtctgcgcgt ctttcagggc ttcatcgaca gtctgacgac cgctggcggc
     9481 gttgatcacc gcagtacgta cggcatacca gaaagcggac atctgcggga tgttcggcat
     9541 gatttcacct ttctgggcgt tttccatggt ggcggcaata cgtggatctt tcgccaactc
     9601 ttcctcgtaa gacttcagcg ctacggcacc cagcggtttg tctttattaa ccgcttccag
     9661 accttcatca gtcagcagat agttttcgag gaactctttc gccagctctt tgttcggact
     9721 ggcggcgtta ataccggtgc tcagcacgcc aacgaacggt ttagatggtt gacccttgaa
     9781 ggtcggcagt accgttacac cataattcac tttgctggtg tcgatgttgg accatgccca
     9841 cgggccgttg atggtcatcg ctgtttcgcc tttattaaag gcagcttctg cgatggagta
     9901 atcggtgtct gcattcatgt gtttgttttt aatcaggtca accaggaagg tcagacccgc
     9961 tttcgcgcca gcgttatcca cgcccacgtc tttaatgtcg tacttgccgt tttcatactt
    10021 gaacgcataa cccccgtcag cagcaatcag cggccaggtg aagtacggtt cttgcaggtt
    10081 gaacatcagc gcgctcttac ctttcgcttt cagttcttta tccagcgccg ggatctcttc
    10141 ccaggttttt ggcgggttcg gcagcaggtc tttgttataa atcagcgata acgcttcaac
    10201 agcgattggg taagcaatca gcttgccgtt gtaacgtacg gcatcccatg taaacggata
    10261 cagcttgtcc tggaacgctt tgtccggggt gatttcagcc aacaggccag attgagcgta
    10321 gccaccaaag cggtcgtgcg cccagaagat aatgtcaggg ccatcgccag ttgccgcaac
    10381 ctgagggaat ttctcttcca gtttatccgg atgctcaacg gtgactttaa ttccggtatc
    10441 tttctcgaat ttcttaccga cttcagcgag accgttatag cctttatcgc cgttaatcca
    10501 gattaccagt ttaccttctt cgattttggc gagagccgag gcggaaaaca tcatcgtcgt
    10561 taatgcggat aatgcgagga tgcgtgcacc tgtttttatt ttcataatct atggtccttg
    10621 ttggtgaagt gctcgtgaaa acacctaaac ggactctagt ttctttatac ggcaacctct
    10681 ttccatcctc cttgccccta cgccccaccg ttgctttgtg tgatctctgt tacagaattg
    10741 gcggtaatgt ggggatgcgc acataaaatc gccacgattt ttgcaagcaa catcacgaaa
    10801 ttccttacat gacctcggtt tagttcacag aagccgcgtt ctcatcctcc cgcctcctcc
    10861 cccataaaaa agccaggggg tggaggattt aagccatctc ctgatgacgc atagtcagcc
    10921 catcatgaat gttgctgtcg atgacaggtt gttacaaagg gagaagggca tggcgagcgt
    10981 acagctgcaa aatgtaacga aagcctgggg cgaggtcgtg gtatcgaaag atatcaatct
    11041 cgatatccat gaaggtgaat tcgtggtgtt tgtcggaccg tctggctgcg gtaaatcgac
    11101 tttactgcgc atgattgccg ggcttgagac gatcaccagc ggcgacctgt tcatcggtga
    11161 gaaacggatg aatgacactc cgccagcaga acgcggcgtt ggtatggtgt ttcagtctta
    11221 cgcgctctat ccccacctgt cagtagcaga aaacatgtca tttggcctga aactggctgg
    11281 cgcaaaaaaa gaggtgatta accaacgcgt caaccaggtg gcggaagtgc tgcaactggc
    11341 gcatttgctg gatcgcaaac cgaaagcgct ctccggtggt cagcgtcagc gcgtggcgat
    11401 tggccgtacg ctggtggccg agccaagcgt atttttgctc gatgaaccgc tctccaacct
    11461 cgatgctgca ctgcgtgtgc aaatgcgtat cgaaatctcc cgtctgcata aacgcctggg
    11521 ccgcacaatg atttacgtca cccacgatca ggtcgaagcg atgacgctgg ccgacaaaat
    11581 cgtggtgctg gacgccggtc gcgtggcaca ggttgggaaa ccgctggaac tgtaccacta
    11641 tccggcagac cgttttgtcg ccggatttat cggttcgcca aagatgaact tcctgccggt
    11701 aaaagtgacc gccaccgcaa tcgatcaagt gcaggtggag ctgccgatgc caaatcgtca
    11761 acaagtctgg ctgcctgttg aaagccgtga tgtccaggtt ggagccaata tgtcgctggg
    11821 tattcgcccg gaacatctac tgccgagtga tatcgctgac gtcatccttg agggtgaagt
    11881 tcaggtcgtc gagcaactcg gcaacgaaac ccaaatctat atccagatcc cttccattcg
    11941 tcaaaacctg gtgtaccgcc agaacgacgt ggtgttggta gaagaaggtg ccacattcgc
    12001 tatcggcctg ccgccagagc gttgccatct gttccgtgag gatggcactg catgtcgtcg
    12061 actgcataag gagccgggcg tttaagcacc ccacaaaaca cacaaagcct gtcacaggtg
    12121 atgtgaaaaa agaaaagcaa tgactcagga gatagaatga tgattactct gcgcaaactt
    12181 cctctggcgg ttgccgtcgc agcgggcgta atgtctgctc aggcaatggc tgttgatttc
    12241 cacggctatg cacgttccgg tattggctgg acaggtagcg gcggtgaaca acagtgtttc
    12301 cagactaccg gtgctcaaag taaataccgt cttggcaacg aatgtgaaac ttatgctgaa
    12361 ttaaaattgg gtcaggaagt gtggaaagag ggcgataaga gcttctattt cgacactaac
    12421 gtggcctatt ccgtcgcgca acagaatgac tgggaagcta ccgatccggc cttccgtgaa
    12481 gcaaacgtgc agggaaaaaa cctgatcgaa tggctgccag gttccaccat ctgggcaggt
    12541 aagcgcttct accaacgtca tgacgttcat atgatcgact tctactactg ggatatttct
    12601 ggtcctggtg ccggtctgga aaacatcgat gttggcttcg gtaaactctc tctggcagca
    12661 acccgctcct ctgaagctgg tggttcttcc tcttttgcca gcaacaatat ttatgactat
    12721 accaacgaaa ccgcgaacga cgttttcgat gtgcgtttag cgcagatgga aatcaacccg
    12781 ggcggcacat tagaactggg tgtcgactac ggtcgtgcca acctgcgtga taactatcgt
    12841 ctggttgatg gcgcatcgaa agacggctgg ttattcactg ctgaacatac tcagagtgtc
    12901 ctgaagggct ttaacaagtt tgttgttcag tacgctactg actcgatgac ctcgcagggt
    12961 aaaggtctgt cgcagggttc tggcgtcgcg tttgataacg aaaaatttgc ctacaatatc
    13021 aacaacaacg gtcacatgct gcgtatcctc gaccacggtg cgatctccat gggcgacaac
    13081 tgggacatga tgtacgtggg tatgtaccag gatatcaact gggataacga caacggcacc
    13141 aagtggtgga ccgttggtat tcgcccgatg tacaagtgga cgccaatcat gagcaccgtg
    13201 atggaaatcg gctacgacaa cgtcgaatcc cagcgcaccg gcgacaagaa caatcagtac
    13261 aaaattaccc tcgcacaaca atggcaggct ggcgacagca tctggtcacg cccggctatt
    13321 cgtgtcttcg caacctacgc caagtgggat gagaaatggg gttacgacta caacggcgat
    13381 agcaaggtta acccgaacta cggcaaagcc gttcctgctg atttcaacgg cggcagcttc
    13441 ggtcgtggcg acagcgacga gtggaccttc ggtgcccaga tggaaatctg gtggtaatag
    13501 caaaacctgg gccggataag gcgtttacgc cgcatccggc ataaaaacag gttgtcatta
    13561 tctgaaaggg gcgaaagccc ctctgattat cgggtttagc gcgctattgc ctggctaccg
    13621 ctgagctcca gattttgagg tgaaaacaat gaaaatgaat aaaagtctca tcgccctctg
    13681 tttatcagca gggttactgg caagcgcgcc tggaattagc cttgccgatg ttaactacgt
    13741 accgcaaaac accagcgacg cgccagccat tccatctgcc gcgctgcaac aactcacctg
    13801 gacaccggtc gatcaatcta aaacccagac cacccaactg gcgaccggcg gccaacaact
    13861 gaacgttccc ggcatcagtg gtccggttgc tgcgtacagc gtcccggcaa acattggcga
    13921 actgaccctg acgctgacca gcgaagtgaa caaacaaacc agcgtttttg cgccgaacgt
    13981 gctgattctt gatcagaaca tgaccccatc agccttcttc cccagcagtt atttcaccta
    14041 ccaggaacta ggcgtgatga gtgcagatcg gctggaaggc gttatgcgcc tgacaccggc
    14101 gttggggcag caaaaacttt atgttctggt ctttaccacg gaaaaagatc tccagcagac
    14161 gacccaactg ctcgacccgg ctaaagccta tgccaaaggc gtcggtaact cgatcccgga
    14221 tattcccgat ccggttgctc gtcataccag cgatggctta ctgaaactga aagtcaaaac
    14281 gaactccagc tccagcgtgt tggtaggacc gctatttggt tcttccgctc cagctccggt
    14341 tacggtaggc aacacggcgg caccagctgt ggctgcacct gctccggtgc cggtgaagaa
    14401 aagcgagccg atgctcaacg acacggaaag ttattttaat accgcgatca aaaacgctgt
    14461 cgcgaaaggt gatgttgata aggcgttaaa actgcttgat gaagctgaac gcctgggatc
    14521 gacatctgcc cgttccacct ttatcagcag tgtaaaaggc aaggggtaat tacgccccac
    14581 agtgctgatt ttgcaacaac tggtgcgtct cctggcgcac ctttttttat gcttccttcc
    14641 tgggatatga gcgatttttt atagtacttc acttcttctt cactaagaat gtccattatt
    14701 tcaatgcatt atcagagatt cttttccttt cgccggtagt gtctggacgc tcaggctact
    14761 tttccaggtt attttatttc tgtattacag aggttttatg ataggtcata tcacaaattc
    14821 tggcggcaat aactctttga tgaaacatga tgtggtgcaa ggaaataata tagtagatct
    14881 tgatttacta cgtaatttc
//
LOCUS       DAZSIO010000096        14713 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZSIO010000096 DAZSIO010000000
VERSION     DAZSIO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.096"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(17..1279)
                     /locus_tag="ACHX5T_002658"
     CDS             complement(17..1279)
                     /locus_tag="ACHX5T_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8467805.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(1477..1552)
                     /locus_tag="ACHX5T_002659"
     tRNA            complement(1477..1552)
                     /locus_tag="ACHX5T_002659"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1517..1519),aa:Phe,seq:gaa)
     gene            complement(1658..2365)
                     /locus_tag="ACHX5T_002660"
     CDS             complement(1658..2365)
                     /locus_tag="ACHX5T_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR8467806.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2518..2601
                     /gene="yqgH"
                     /locus_tag="ACHX5T_002661"
     CDS             2518..2601
                     /gene="yqgH"
                     /locus_tag="ACHX5T_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR8467807.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2763..4898
                     /gene="speC"
                     /locus_tag="ACHX5T_002662"
     CDS             2763..4898
                     /gene="speC"
                     /locus_tag="ACHX5T_002662"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR8467808.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4947..6203)
                     /gene="nupG"
                     /locus_tag="ACHX5T_002663"
     CDS             complement(4947..6203)
                     /gene="nupG"
                     /locus_tag="ACHX5T_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR8467809.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6405..7484)
                     /gene="mltC"
                     /locus_tag="ACHX5T_002664"
     CDS             complement(6405..7484)
                     /gene="mltC"
                     /locus_tag="ACHX5T_002664"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR8467810.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7549..7824)
                     /locus_tag="ACHX5T_002665"
     CDS             complement(7549..7824)
                     /locus_tag="ACHX5T_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR8467811.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7852..8904)
                     /gene="mutY"
                     /locus_tag="ACHX5T_002666"
     CDS             complement(7852..8904)
                     /gene="mutY"
                     /locus_tag="ACHX5T_002666"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR8467812.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9065..9784
                     /gene="trmB"
                     /locus_tag="ACHX5T_002667"
     CDS             9065..9784
                     /gene="trmB"
                     /locus_tag="ACHX5T_002667"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR8467813.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9784..10110
                     /locus_tag="ACHX5T_002668"
     CDS             9784..10110
                     /locus_tag="ACHX5T_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR8467814.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10294..11013
                     /locus_tag="ACHX5T_002669"
     CDS             10294..11013
                     /locus_tag="ACHX5T_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR8467815.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11189..12235
                     /gene="ansB"
                     /locus_tag="ACHX5T_002670"
     CDS             11189..12235
                     /gene="ansB"
                     /locus_tag="ACHX5T_002670"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR8467816.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12352..13314
                     /locus_tag="ACHX5T_002671"
     CDS             12352..13314
                     /locus_tag="ACHX5T_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR8467817.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(13415..>14713)
                     /locus_tag="ACHX5T_002672"
     CDS             complement(13415..>14713)
                     /locus_tag="ACHX5T_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFR8467818.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3836 a         3553 c         3793 g         3531 t
ORIGIN      
        1 ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca aacgggctga
       61 tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc agtcgccgtt
      121 cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc tcctgatggc
      181 tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat gcactgcacg
      241 ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc atcaccctca
      301 gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca cccgtaaaga
      361 tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca acctgtttgc
      421 tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat tttacgcctg
      481 gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca atttccgacc
      541 agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc ataactgcca
      601 gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca ggcaggcgtg
      661 aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca gccatatcca
      721 gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca gcagatattc
      781 tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca cgaacaggga
      841 taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag gggaaaacgt
      901 gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt ttattactgg
      961 catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca acttttttaa
     1021 ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc tcatcccgtt
     1081 ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga gtcttctcct
     1141 tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga accagcagga
     1201 acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca gcccggattt
     1261 ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt acaaattctg
     1321 tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg ggggaatctg
     1381 gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt tctgaaacga
     1441 aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc ggaatcgaac
     1501 caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc
     1561 gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc
     1621 acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg
     1681 cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt
     1741 taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt
     1801 ctgccatcat cgacggtgtg atcagcggta atatcagcgc ggcagcccac gccagcgtta
     1861 actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc gagcaggcga
     1921 agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc ggatcgccgg
     1981 tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa
     2041 tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag tgacgaaaca
     2101 gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa atttcgccaa
     2161 ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat ttcacaacca
     2221 gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac
     2281 gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag
     2341 cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt tgattttctt
     2401 attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc
     2461 ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg
     2521 cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct
     2581 atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca gtaaaatcag
     2641 agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt gacccatatc
     2701 tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac gggtttgcgc
     2761 ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt tcttctcatc
     2821 gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg
     2881 ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg
     2941 tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg
     3001 gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc
     3061 tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc acctttgctt
     3121 gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg
     3181 atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg
     3241 atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc
     3301 atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga
     3361 atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc
     3421 atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct tcacgtaacc
     3481 cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc
     3541 aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc ctgacgatta
     3601 ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccattg
     3661 ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttatct
     3721 cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggattt
     3781 ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg cagatccata
     3841 aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg ttgaataacg
     3901 cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca ctggatgtta
     3961 acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg
     4021 gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc
     4081 ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc agcgaccgcc
     4141 gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc
     4201 agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat gccgaaaccg
     4261 gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg cgtgagaacg
     4321 gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa
     4381 gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag catattgagg
     4441 atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc
     4501 gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg
     4561 tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaacc
     4621 cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg
     4681 ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg
     4741 tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg
     4801 tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg
     4861 atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt
     4921 ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca
     4981 cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggaataacca
     5041 gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca
     5101 actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac
     5161 atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca
     5221 gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca
     5281 gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca
     5341 atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga
     5401 atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg
     5461 aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg
     5521 ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat tgccatacgc
     5581 ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct
     5641 tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa
     5701 agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc
     5761 cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg
     5821 atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt
     5881 ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc
     5941 tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc
     6001 gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac cgcggcgata
     6061 cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata
     6121 taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt
     6181 ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga tgagtgtttg
     6241 aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt
     6301 tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg
     6361 ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag
     6421 gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg
     6481 gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga
     6541 atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg
     6601 acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt gttcagcatc
     6661 gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc
     6721 ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt ttgtaccacc
     6781 tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga agattcagtc
     6841 tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc ctggcggacc
     6901 atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt aatggtgacg
     6961 ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag cagataatcc
     7021 gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga
     7081 ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata gagatcgacc
     7141 gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa atgcgcggca
     7201 ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg
     7261 cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat caccacctct
     7321 ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg
     7381 ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag
     7441 atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt
     7501 tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt attttttatc
     7561 ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt tgaccatctc
     7621 ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt cattaatcag
     7681 catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt tatagatgcg
     7741 ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac gttgcaggaa
     7801 agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc gctaaaccgg
     7861 cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc caactgacgg
     7921 cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg tgaatgacga
     7981 cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc
     8041 ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt gccagccaat gccgcaaact
     8101 ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc tcggcggacg
     8161 ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg tgcgctccgg
     8221 cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg tggcggcaat
     8281 acatccgttt tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc aaatcatcgc
     8341 gcccaaatcc atcatcgcct gattaaaccg ttccacgccg accgcgggcg tcacctgctc
     8401 gctcaaactc catagtttat tctcgacctc ttttttccca ggccagccgc ttacagcata
     8461 gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct tacccagaga
     8521 aagcgagaga atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg cgacttcttc
     8581 aaaggtttcc gggaatttac caccgtgtaa ggtcgccact tgttgtgccg ctttatgcag
     8641 gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt cgtcgagcgg
     8701 cgcattggcg agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa aataggggat
     8761 aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt tgtagggcgt
     8821 cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt ccagaacctg
     8881 ggctgaaaat tgcgacgctt gcatggtcac cgacttcact gttgttgggg gcaagattgc
     8941 agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt atcatgcttg
     9001 catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa gcgactaaat
     9061 ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc cactgcgccg
     9121 tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac atgcgctgga
     9181 aaactactgg ccggtgatgg gcgttgagtt cagtgaagat atgctggatt tccccgcgct
     9241 ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg cgtcgctggt
     9301 ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc attcaccggg
     9361 cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc gcgtgatgtg
     9421 tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc gcatggtgca
     9481 gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta tcgttcaggt
     9541 gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggcgtattcc atatggcgac
     9601 cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg acggttataa
     9661 aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg tgacgaaatt
     9721 tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg agagggtgaa
     9781 ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag
     9841 gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga acagattgat
     9901 aaaatcgttg atgactttat taacgaggtt atcgaaccga acaaactggc ctttgacggc
     9961 agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa
    10021 gaacatcagg cgattgtgcg taagtggctg gaagagtgca aactggatga ggtacgcacc
    10081 agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac aaatgcaaaa
    10141 ctgcctgatg cgctacgctt atcaggccta cagagatgca cgatcgagta ggcgggataa
    10201 ggcgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct ggggccagca
    10261 tatgctggcc tgatttgttc ttgagggaag actatgatgc gcaaaatgct gctggcggca
    10321 gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt cacgccgcgt
    10381 gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg
    10441 atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa tgccgcccag
    10501 cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg gattgatgaa
    10561 ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg ataaaattat cgttcaggag
    10621 atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca gcttaaagag
    10681 cagatgaatc gcattatcga aacgcgcagc gatggcctga cgttccacta taaagccatt
    10741 gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg aattttacag
    10801 gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa cccattacag
    10861 aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg gaaaaagcag
    10921 gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac tctggaagat
    10981 agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg gcataatact
    11041 tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag atctccaaaa
    11101 tatattcacg ttgtaaattg tttaacgtca aatttcccat gcaaagctaa gggataatgc
    11161 gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc
    11221 actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca ttttagcaac
    11281 cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa
    11341 agttggcgta gaaaatctgg ttaatgcggt gccgcaacta aaagacattg cgaacgttaa
    11401 aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct ggctgacact
    11461 ggcgaaaaaa attaacgccg actgcgataa aaccgacggc ttcgtcatta cccacggtac
    11521 cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg acaaaccggt
    11581 ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc cattcaacct
    11641 gtataacgcg gtagtgaccg cagctgataa agcctccgct aatcgtggcg tgctggtggt
    11701 gatgaacgac accgtactgg acggtcgcga tgtcaccaaa accaacacca ccgacgtagc
    11761 gaccttcaag tctgttaact acggtcctct gggatacatt cacaacggta agattgatta
    11821 ccaacgtacc ccggcacgta agcacaccag cgatacgcca ttcgatgtct ctaagctgaa
    11881 tgagctgccg aaagtcggca tcgtttataa ctacgctaac gcatccgatc ttccggctaa
    11941 agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta atggtaacct
    12001 gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg cagtagtgcg
    12061 ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct gaagtggatg atgcgaaata
    12121 cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc tgcagctggc
    12181 tctgacgcaa accaaagatc cgcagcagat ccagcagatt ttcaatcagt actaatcacc
    12241 tcgtcccggc atcgtgccgg ggctttttca cttcagactt acgtccattg ccaattttca
    12301 ttaccctaat gataatcacc ggaataaatt attccgcgcg agggttttcg ggtgaaaaag
    12361 caatggattg ttggcacggc gctgcttatg ttgatgactg gtaatgcctg ggcagatggc
    12421 gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca cggcattctc
    12481 aagcttgatg ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc cacctggtca
    12541 gcggaaggcg atgtctcttc cagtgacgat ctctatacct gggtcggtca gttggcagat
    12601 tacgaactgc tcgaacagac ctggacgaaa gataaaccgg taaaattctc ggcgatgtta
    12661 accagtaaag gaacgcccgc gtctggctgg tcggtgaact tttactcttt tcaggcggca
    12721 gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga ataataaata tctgatcgtg
    12781 aatagcgaag atttcaatta tcgctttagt cagcttgaat ctgcgttgaa tacccagaag
    12841 aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg
    12901 cagaaagcag cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa
    12961 gaagccttta aaaaaattca ccaacagcgt gatgagttta ataaacagaa cgatagcgag
    13021 gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg cgattgcggc atgtcataaa
    13081 cagagtgaag agtgttatga agtgccgatt cagcagaaac gagatttcga tatcaacgaa
    13141 caacggcgac agacttttct gcaatcacaa aaactcagcc gtaaattgca ggatgactgg
    13201 gtaacgctcg aaaaagggca atatccgtta acgatgaaag tttcagaaat aaacagtaaa
    13261 aaagtcgcga ttttgatgaa aattgacgat atcaaccaag ctaatgaacg ctagaaaaaa
    13321 gataccgaac aactgcggcg caatggggtg attaagtaac tcatgttaag tgcctttgcc
    13381 ggataatata aaaagttatc cggcaaatta ttttttacat tagccctagt agctgtggaa
    13441 tgaacaagga tatttgcgga atgtaagtga tcatcatcaa cgctactagc aatgcagcat
    13501 aaaatagcag caatggtttg attaaatgtt gaatcttcac acccgagata gaacaaccaa
    13561 caaatagcgc actacccacc ggcggtgtta acagcccaat acaaaggttg gccaccatca
    13621 taataccgaa atgtactggg tccattccca gctcttgtgc aatcggcaag aagatcggtg
    13681 taaagattag tacagccggt gtcatgtcca taaagatgcc gacaattaat aagacgatat
    13741 taatcagcag caatatgatc agtgggttat cagaaatccc catcaatgcg tcgctgatca
    13801 tataaggaat atcagcatta gtcattgccc aggacatgcc cacggaaaaa ccgatcaaca
    13861 gtaatacaat cgaagtggta acaaccgact ccagaatcag tttgggtaaa tggcgccatt
    13921 ttacttcccg gtaaatcagc accgagagaa taaatgtgta gacaacggca attgctgacg
    13981 cttcagttgc ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac accagaagca
    14041 aacttggtaa ggcatccaga aacgctttac aggcctgagc aaaggttgct ctttctgaaa
    14101 gcgggtagcc acggcgttta gcaataatgc cgcagactat cattaccgca agacccatta
    14161 agatccctgg caaatatccc gccataaaca gcgatgctac agaaacgccg ccagctgtca
    14221 gggaaaacac aattaacacg tttgagggcg gtatcagtaa tccagtaata caagatgaga
    14281 cgtttacagc ggtagaaaat gccggatcat aaccttcttt ggtctgaatg ggattcagtg
    14341 tgccaccaac ggcagcagct gccgcaacgg ctgaaccaga aatggaacca aacatcatat
    14401 tcgccaggac gttcacatga ccaagcgaac caggaacgcg gccaaccatt acctgggcga
    14461 ggttaatcaa acgtatcgca ataccgccgc tgttcatcag tgttccggca aagatgaaaa
    14521 aggggattgc caacagggca aagttatcca gtccattagc cagtcgttgc gaaacagtga
    14581 ttgccgcgat atcccaggga aacatcaaaa gcattgaagc tacagtggca ataccgattg
    14641 agaacgaaat gggcacacca ataaagacca gcacaaaaaa acagccaaac agtgtaatcg
    14701 cgatataact atc
//
LOCUS       DAZSIO010000097        14682 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZSIO010000097 DAZSIO010000000
VERSION     DAZSIO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.097"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(224..1165)
                     /gene="fabY"
                     /locus_tag="ACHX5T_002673"
                     /gene_synonym="yiiD"
     CDS             complement(224..1165)
                     /gene="fabY"
                     /locus_tag="ACHX5T_002673"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR8467819.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1210..1647)
                     /gene="dtd"
                     /locus_tag="ACHX5T_002674"
     CDS             complement(1210..1647)
                     /gene="dtd"
                     /locus_tag="ACHX5T_002674"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR8467820.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1644..2516)
                     /locus_tag="ACHX5T_002675"
     CDS             complement(1644..2516)
                     /locus_tag="ACHX5T_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR8467821.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2510..3109)
                     /gene="yihX"
                     /locus_tag="ACHX5T_002676"
     CDS             complement(2510..3109)
                     /gene="yihX"
                     /locus_tag="ACHX5T_002676"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR8467822.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3208..3993)
                     /locus_tag="ACHX5T_002677"
     CDS             complement(3208..3993)
                     /locus_tag="ACHX5T_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR8467823.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4027..4923)
                     /gene="yihV"
                     /locus_tag="ACHX5T_002678"
     CDS             complement(4027..4923)
                     /gene="yihV"
                     /locus_tag="ACHX5T_002678"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFR8467824.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5091..5987
                     /gene="yihU"
                     /locus_tag="ACHX5T_002679"
     CDS             5091..5987
                     /gene="yihU"
                     /locus_tag="ACHX5T_002679"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR8467825.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6011..6889
                     /gene="yihT"
                     /locus_tag="ACHX5T_002680"
     CDS             6011..6889
                     /gene="yihT"
                     /locus_tag="ACHX5T_002680"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFR8467826.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            6906..8147
                     /gene="yihS"
                     /locus_tag="ACHX5T_002681"
     CDS             6906..8147
                     /gene="yihS"
                     /locus_tag="ACHX5T_002681"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFR8467827.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8285..9187
                     /locus_tag="ACHX5T_002682"
     CDS             8285..9187
                     /locus_tag="ACHX5T_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFR8467828.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9386..11422
                     /gene="yihQ"
                     /locus_tag="ACHX5T_002683"
     CDS             9386..11422
                     /gene="yihQ"
                     /locus_tag="ACHX5T_002683"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFR8467829.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11468..12842
                     /locus_tag="ACHX5T_002684"
                     /pseudo
     CDS             11468..12842
                     /locus_tag="ACHX5T_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12885..14288
                     /locus_tag="ACHX5T_002685"
     CDS             12885..14288
                     /locus_tag="ACHX5T_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8467830.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14356..>14682
                     /locus_tag="ACHX5T_002686"
     CDS             14356..>14682
                     /locus_tag="ACHX5T_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFR8467831.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3571 a         3809 c         3689 g         3613 t
ORIGIN      
        1 caaacctgaa ctaatatgat gagttaagca ttatctgcaa taattgccac tcagctctac
       61 aaataatatt taaatgctcc tgaacagtga tgtagcaaga cacgtatggt gccaacgaag
      121 aataaaaatt tataaagttc ttattcaata taaccggaat agtcagggac tgatgtaaat
      181 gaccggaatg aatgagagag gcagcaccgc ccctctccat aagctactct tcttcgttcc
      241 cgccctcttc atacgggcca aatggcttcg cgggcagaac gatatacgtg ccttcaaaca
      301 ctgcacccgg cgtctcgtcg ccaaagattt cgacctgcat ctgcacccgt gcttttcgtc
      361 cgcgcgccag acggtcgaga tcgccgctta aggcaccaag gtcggctacc gcatggggtt
      421 taccgctaat cggtttgctg tagcggatat gcgcatccgc cagaataatc gttccgccga
      481 ggtggcgttc acgcagcatc agccagataa gcccccaacc ggtgagcgtc gccagcgaga
      541 ataaactccc ggcaaacagc gtatggtgcg gattctgatt gccggtttcc ggcatggtgg
      601 tgataaattt ttgcccggta tattgttgaa tacgcacgcc cattttttca ctaagcggga
      661 tatgttcgta ccacgcctgt tgcagctgcg cgcaccagtc gccgcgatgc aaaatatcat
      721 ccagagtggc gacgggctta atcatcaaaa aatggcgaat cggcgtggtg gttggcgtgg
      781 tgatttctcc ctgattaaca aaccccagct tggcgaaaaa ctccaccgcg tcttcacggg
      841 cgctacaggt cacgcgctta acgccttcct gacgcgccac cgactccagg gtcatcgcca
      901 tcagcgttcc taaccctttg tcctgcacgt ccgggtgaac gaccataaag cgaatggacg
      961 cttcattgtc ggcattaata tacaatcggc ctaccgccac cagattaccc tgctcgtcga
     1021 cgaccatctg gtgatgcgcc atcgcatccc acgcgtcgcg ttccgaacct tttggttgat
     1081 gcaggggctt acgcaacatt tcccagcgaa agtgatagta acgctctaac tcttcttctg
     1141 tttgtggaac ctgaaggtga tacatagcga tactctctct tgtttcccgt gacaaccctg
     1201 gaagctggct catacctgca accagaatgt cacggggcca tcattgacca gcgatacctg
     1261 catatcggca gcgaagcgtc ctgtttgcgt gttcatctct tgctggcggc agcgttcgac
     1321 gaaatagtca tataacgcct ctgcgcgatc cggtgatgcg cctttggaga aacttgggcg
     1381 catcccccgt tcggtatctg cggcgagggt aaactgggaa accaccagta cactgccgcc
     1441 cgcctgttgc acgttgagat tcatcttgcc ttcggcatcg ctaaagatgc ggtagccgag
     1501 cacacgctcg cacagacggt ttgctttctg ttcgtcgtca tccttttcga cacccaataa
     1561 caccaaaagt cccgcgccaa tttcgcccgt cacttctccc tccacggtga cgctggcacg
     1621 ggttacgcgt tgaattaatg caatcatggt tcgtcgtctt cttcttgttc agctgcttgt
     1681 tttagtttgc ggtattcccc gagagtgaca gtaatttccg cgccaagcaa gacgatacac
     1741 catgtccagt agacccaaac aaagagaatg gggatcaccg ccagcacacc gtaaatgagc
     1801 tgatatgacg ggaacatggt gatataaagc gcgaaacctt tctttcctgc ttcgaacagg
     1861 agtgcggcga caaacgcgcc gacaatcgcg tcgcggttag gtacgcggat ggtaggaaca
     1921 atgctgtaca gcaaccagaa ggagatccac gacaacagca acggaaaaat acgcagcacg
     1981 ttgtcgatga cagtattgag atcgctcgcc cagcgcaggg agagcaaata ggaactgatc
     2041 gccagactgg cccctgccag cagcggcccc agcgttaaaa tcatccagta cacggcgaac
     2101 gagtaaattt ttggtcgcgc tcgtttactg cgccagatgg tattcaacgc gctatcgatg
     2161 gagtacatca acaataacgc ggtgacgatc agcccgcacg ccccaacggc ggtcatcttg
     2221 ttggaattag caacaaattg ttcgatatac cgctgaataa catcgccagt agcaggcaga
     2281 aagttggcaa aaataaagtg acgtaactga atgctgacgt cggaaaacat gggaaaagcg
     2341 gcgaaaagcg caaaaacaac ggcaaccagc ggcactaatg agagcaacga cacataggca
     2401 aggttacctg ccagggttgt catgttgtcc tcatcaatac gttgccagag tagttttagc
     2461 caggcccata gtggacgggt acggtgcctg gctttgtcct gaatggtttt tagcataaca
     2521 ccttcgcgaa atagtccggg atggtggttt tatctttcac cagaatactg gtaatgccta
     2581 gctgattagc tccttctata ttatcggcgt tatcgtcgaa aaagaccgta tcgctgggtg
     2641 aaaaaccttc cgcctgcaaa acatgctggt aaattcgtgc ttcaggtttg cgcatcccca
     2701 gatcttgcga cagatagata tggtcagcag catcacgaat ttccgggtat tcttccggcc
     2761 agaaggtggt atgcaggcgg ttggtattgg aaagcaccac cacgcgatgc ccctgctcac
     2821 gcagtttatg catgatggcg atcacttccg ggcgcagcgc aacaaacacc gcctgccagc
     2881 cgtgagagaa ctgctcgtag cttagcggta gagccatctc atgacacagc gcctctgcga
     2941 acgcttcgtc gctaatttcc ccacgctcat gctgatgaaa cgcctccccc atatgaaaac
     3001 tcttcttaag cgatgccagc ggaatacgcg ttaaatcgct ccaggctccc agcacacggt
     3061 taaagtcgat atcgacaatc acattaccta aatcaaagat atagagcatt tttgcctcct
     3121 ttcgcgccac aagaaattaa ctgtagcggg aaaggatggg tttggctatg tggcaaggta
     3181 tgaagccagg ttaacggtca caaaaatcta cgcgtcttcc tggggaagaa tgatttcaat
     3241 ttggctgtct tgtaagcgtg attttagcgc agcgggcggc agctcgtcgg taaagagcgc
     3301 agtgacctgt gccacgttac caatttcaac cgccgccgaa gcatgatact tagtgtgatc
     3361 ggcgaccagc agaatatttc tcgcgtgcgc catcatcgtt ttcaccacgt tagcttcgtt
     3421 tacatcaaac tccatcaacg cgccatcgct ctcaatcgcc ccaacgcttg ttaccagata
     3481 atcagcacga aaatcagcca caaaggacgc cgctgaaggg ccaatgatcc cgctattatg
     3541 agagcgcaac gtaccgccgg gcaccatcac ttcaaagcgc gggttgtggt aaagaatatg
     3601 cgccacacgc aggctgttgg tgattatccg caaatgatta tggttaagta acgcccgggc
     3661 aacatgctca acagtcgtac caatggtgat aaatattgtt gaaccatcag gaatatagtc
     3721 tgccaccgct tcggcaatcg cttttttttt ctcggtttgc gaaacctcac gctgctcgaa
     3781 cgccgtatta acgacgctgg aagcccgacc cgcgccaccg tgatggcgcg taatcaggcc
     3841 ttgctcgctt aatttacgaa tatcccggcg gaccgtctgc gtggagacat ccagcagatt
     3901 tgccagctca tcaatattca tatacccacg ctcggcgatc agcatgagga gttggtcgtg
     3961 ccgcgggtta ccggttagtt cggtaaggct catgaaaatt cctcgaaaaa ccatcaccct
     4021 ggcattttat acaaaaagtg acaaaaaaga tcgggtttga tcacagtcag ggatcccggc
     4081 tcgtccaccg ggacgtgtgc attttaacgc cgctacaccg ctggcgaagc ggactgactc
     4141 cgctaaatcc ccacttgttg ccagcgccac cgccaaagcg ccgtgaaaaa catctcccgc
     4201 acctgtggta tctaccacat caactttgaa ggccggttga tgctgacgcc caccattttc
     4261 cagccagtcg cagcctgcgc taccctgggt cacatagaca tgaccatttg tgagcgtttg
     4321 tgcctgtttt agcgcactgg ccatctcttt cacgcccgtt aagcgcgcca gacccggttc
     4381 tgaaaaggcc gcgtgatcgc ttaatgccac cagctcactg atatcctgcg gcgtaatgtc
     4441 cccgtccaga acggtcatca cacccgcctg acgggccagg gtgaaggctt ttttagcgcc
     4501 gtcgtgccag cgtacatctg ccagcacaac atcccactga gagaaatcaa tttcctccaa
     4561 ccactctgcg tcaggcagca gatccgggct ggggtagtta atgattatcc gctcgccttt
     4621 ggtatccacc atgatggcgg attgcgaaga tttcgcctgg ttataccgtt tggtgtaacg
     4681 ggtgttaacg ccccaggatt ccagctctgc cagcaggctg ttgcccgcgt cgtcatcacc
     4741 tacgcgacca ataaaatcga cctgcgcccc cagccttgcc gccgcaaccg ctgccgtcgc
     4801 cgctggcccg ccaccaactt ccgtataatt tctcgccacg tatttaccgc tctccgtcgg
     4861 taacccttcc acgtaataga tgcgatccat cacggttata cctacacaag caacacgaat
     4921 catggtcatt ccttaaacat tttgggatgc gttcatttta atttcaccaa ttgtttaaaa
     4981 agtgacggct gtcaattttt tgacgataaa ttacaaattc gatcaaaaac agacaagcaa
     5041 agcatgtcat aaatgacaaa aagtgacatc actgtattca ggagagggtt atggcagcaa
     5101 tcgcgtttat cggtttagga caaatgggtt cgccaatggc gagcaattta ttgcagcaag
     5161 ggcatcaact tcgcgtcttt gatgtgaacg ccgaagctgt gcggcatctg gtagacaaag
     5221 gtgcgactcc cgccgccaac ccggcgcagg ccgctaaaga tgccgaattt atcattacca
     5281 tgttgccgaa tggcgatctg gtgcgcaacg tgttgttcgg tgaaaacggc gtttgcgaag
     5341 gcttatctac cgatgcgctg gtcattgata tgtccaccat ccatccgctg caaaccgata
     5401 aattgattgc cgatatgcaa gccaaaggct tcagcatgat ggatgttccg gtaggccgta
     5461 cttctgcaaa tgccattacc ggtactctgt tactgctggc tggcggcacc gctgaacaag
     5521 ttgaacgtgc cacaccgatc ctgatggcga tgggcagtga gttgatcaac gctggcggtc
     5581 cgggcatggg gatccgcgtt aagctcatca acaactacat gagcatcgcg ctcaatgcgc
     5641 tttcggcaga agccgccgtt ttgtgcgaag ccctgaatct tcccttcgat gttgccgtca
     5701 aagtgatgag cggtaccgcc gccggtaaag gtcacttcac cacttcctgg ccaaacaaag
     5761 tcctcagcgg agatctttct cccgccttca tgatcgatct tgcccataag gatcttggca
     5821 tcgcccttga tgtcgccaac cagctgcatg tgccaatgcc gctgggggcc gcctcacggg
     5881 aggtttatag ccaggcgcgc gcagcgggtc gcggtcgcca ggactggtcc gccattctgg
     5941 aacaggtccg tgtcagtgcc gggatgactg ccaaagtaaa aatgtaacga ctggataaag
     6001 gaataaatga atgaataagt acaccatcaa cgacattacg cgcgcatcgg gcggttttgc
     6061 catgctggcg gtcgatcagc gcgaagccat gcgcatgatg tttgccgcgg ctggggcacc
     6121 cgctccggta gccgatagcg ttttaactga tttcaaagtt aacgctgcaa aggccctctc
     6181 gccttatgcc tcggcgattc tggtagatca acaattctgc taccgccagg tggttgagca
     6241 aaacgcgatt gccaaaagtt gcgccatgat tgtcgccgcc gatgagttca ttcctggcaa
     6301 cggtattccg gtcgatagcg tggttattga ccgcaaaatc aatccgctac agatcaaaca
     6361 agacggtggc aaagccttaa aactgctggt gctgtggcgt agtgatgaag atgcgcagca
     6421 acgtctggat atggtgaaag agttcaacga actgtgccac tcacacggtc tggtaagcat
     6481 cattgagcca gtcgtccgcc caccgcgtcg tggcgataaa ttcgatcgcg aacaagcgat
     6541 catcgatgcc gccaaagagc tgggcgacag tggcgctgac ctctacaaag ttgaaatgcc
     6601 gctttatggc aaaggtccgc aacaagagct tctctgtgct tcacaacgtc tgaatgacca
     6661 tatcaatatg ccatgggtga tcctctcttc cagtgtcgac gaaaaactgt tcccgcgtgc
     6721 cgtacgcgtg gcaatgacgg cgggcgcatc gggattcctg gcaggccgtg cagtctgggc
     6781 atcggtcgtc ggtttaccag acaacgagct gatgctccgt gacgtttgcg caccgaaatt
     6841 acaacaactt ggcgatatcg tcgacgaaat gatggctaaa cgccgctaag atgaggataa
     6901 aaggaatgaa atggtttaac accctaagcc acaaccgttg gctggaacag gaaaccgacc
     6961 gcatctttga ttttggtaaa aattccgtag tgccgactgg ttttggctgg ttaggcaata
     7021 aagggcaaat caaagaagag atgggcaccc atctgtggat cacagctcgt atgttgcacg
     7081 tttattccgt tgctgcggcg atgggtcgac ctggcgctta cgcgttggtt gatcacggta
     7141 ttaaagccat gaacggcgca ctgcgtgata aaaaatatgg cggctggtat gcctgcgtga
     7201 atgacgaggg cgtggtggat gcctccaaac agggctatca acacttcttt gctctgctgg
     7261 gtgccgccag cgccgtcaca acgggtcacc cggaagcgcg caagctgctc gattacacca
     7321 ttgagattat cgagaaatat ttctggagcg aagaagagca gatgtgcctg gagtcctggg
     7381 acgaagcctt cagcaaaacc gaagagtacc gcggcggcaa tgccaatatg cacgcggtgg
     7441 aagctttctt gattgtttat gacgtcactc acgacaaaaa atggctggat cgcgcgattc
     7501 gcgtggcttc cgtgattatc cacgacgtcg ccagaaataa tcattatcgc gttaacgaac
     7561 acttcgatac ccagtggaat ccgctgccgg attacaacaa agataacccg gcgcatcgct
     7621 tccgcgcgtt cggaggtaca ccaggccact ggatcgaatg gggccgttta atgctgcaca
     7681 tccatgctgc cctggaagcc cgttgcgaac aaccaccagc atggctgcta gaagatgcca
     7741 aaggtctgtt taacgccacc gtgcgcgatg cctgggcacc cgatggagcg gacgggattg
     7801 tttataccgt tgactgggaa ggaaaaccgg tggtccgcga acgtgtacgt tggcctatcg
     7861 tcgaagcaat gggtacggtc tacgcgctct acaccgtcac tggcgatcgc cagtatgaaa
     7921 cctggtatca aacatggtgg gagtactgca ttaagtacct gatggactat gaaaatggtt
     7981 cctggtggca ggagctggat gcggacaata aggtcatcac caaagtctgg gacggcaaac
     8041 aggatattta tcacctgctg cattgcctgg tgatcccgcg tatcccgtta gcgcccggca
     8101 tggctccagc ggttgcagcg ggtctgctgg atattaatgc gaaataactg atctgaaaat
     8161 agggtgcggg ttttcccctc accctaaccc tctccccaaa ggggcgaggg gaccgtccgg
     8221 tgcagttttt gggtttgaca tcagcctcac gggaggctgt atgtcgttaa ttaaggtgcc
     8281 ttgtatgcaa atcaccaata tgcattgcag tggacagacc gtttcacttg ccgcggggga
     8341 ttatcacgcg accattgtga ccgtcggtgc tggtctggct gaattaacct ttcagggttg
     8401 tcatttagtc atcccgcaca aacccgaaga gatgccgctg gcgcatctgg gtaaagtgct
     8461 tattccgtgg ccgaaccgga tcgccaacgg ctgttaccgt tatcaggggc aggaatatca
     8521 gttacccatt aatgaacata gttcaaaagc cgctattcac gggctgcttg cctggcgaga
     8581 ctggcaaatt agtgaattaa ccaccaccag cgttaccctg acagcttttc tgccgcccag
     8641 ttacggctat ccgtttatgc tggcttcaca agttgtttat tcgctcaacg cacacacggg
     8701 tctttccgtt gagatcgcaa gccagaatat cggcactgtc gcagcgcctt atggcgtggg
     8761 tattcatcct tatttaacct gcaacttaac atctgttgat gaatatctgt tccagcttcc
     8821 ggcaaaccag gtttatgccg tcgatgaaca cgtaaatccg acaacgcttc accacgttga
     8881 tgagctggat ttaaatttca cgcaggcgaa aaaaatagcc gcgacaaaaa tcgaccacac
     8941 ctttaaaacc gctaatgatt tgtgggaaat gaccattacc catccacagc aggcgctttc
     9001 ggtaagcctt tgttccgatc aaccctgggt gcaggtttat agcggtgaaa aattacagcg
     9061 tcagggactg gcagttgaac ctatgagttg cccgcctaac gcctttaatt caggtatcga
     9121 cttattatta ctggaaccag gaaaaccgca ccgcctgttt tttaatattt acggacagag
     9181 aaaataactt gtattgatta gcagtcaaaa tcattgaaga acatttcact cgctaaaggc
     9241 aacattatgt atcttcaatt tattaagtta aaaacagctc agcttctgta aagtttttat
     9301 atatcagttt tatgagaatc attttacata agcctgtaat aacaggaagg caacaatctc
     9361 gttctcaact ctatggagat tagttatgga tacgccacgt ccacagttat tagattttca
     9421 atttcatcag aataacgaca gttttaccct acattttcaa caacgtctta ttttaaccca
     9481 taacaaagat aatccttgtt tatggattgg ctcaggtata gcggatatcg atatgttccg
     9541 cggtaatttc agcattaaag ataaactaca ggagaaaatt gcgcttaccg acgccatcgt
     9601 cagccagtca ccggatggtt ggttaattca tttcagccgt ggttctgaca ttagcgccac
     9661 gctgaatatc tctgccgacg atcagggccg tttattgctg gaactacaaa acgacaacct
     9721 taaccacaac cgtatctggc tgcgccttgc cgctcaacca gaggaccata tctacggctg
     9781 cggcgaacag ttttcctact tcgatctgcg cggcaaaccg ttcccgctat ggaccagtga
     9841 acaaggcgtt ggtcgcaaca aacaaaccta tgtcacctgg caggccgact gcaaagaaaa
     9901 tgcgggcggc gactattact ggactttctt cccacagcct acgtttgtca gcacgcagaa
     9961 gtattactgc catgttgata acagttgcta catgaacttc gactttagcg ccccggaata
    10021 ccatgaactg gcgctgtggg aagacaaagc tacgctacgt tttgaatgtg ctgacacata
    10081 catctccctg ctggaaaaat taactgccct gctgggacgc cagccagaac tgcccgactg
    10141 gatttatgac ggggtaacgc tcggcattca gggcgggacg gaagtgtgcc agaagaaact
    10201 ggacaccatg cgtaacgcgg gcgtgaaggt taacggcatc tgggcgcagg actggtctgg
    10261 gatccgtata acctcttttg gcaagcgcgt gatgtggaac tggaagtgga acagcgaaaa
    10321 ctacccgcaa ctggattcac gcattaagca gtggtatcag gagggcgtgc agttcctggc
    10381 ctatatcaac ccgtatgttg ccagcgataa agatctctgc gaagaagcgg cacaacacgg
    10441 ctatctagca aaagatgcct ctggcggtga ctatctggtg gagtttggcg agttttacgg
    10501 cggcgttgtc gatctcacta atccagaagc ctacgcctgg ttcaaggaag tgatcaaaaa
    10561 gaacatgatt gaactcggct gcggcggctg gatggctgac ttcggcgagt atctgcccac
    10621 cgacacgtac ctgcataacg gcgtcagtgc cgaaattatg cataacgcct ggcctgcgct
    10681 gtgggcgaag tgtaactacg aagcccttga agaaacgggc aagctcggcg agatcctctt
    10741 ctttatgcgc gccggttcta ccggtagcca gaaatactcc accatgatgt gggcgggcga
    10801 ccagaacgtg gactggagtc tcgacgatgg cctggcgtcc gtagtcccgg cggcgctgtc
    10861 gctggcaatg accggacatg gtctgcatca cagcgacatt ggcggttaca ccaccctgtt
    10921 tgagatgaag cgcagcaaag agctgctgct gcgctggtgc gatttcagcg ccttcacgcc
    10981 gatgatgcgc acccacgaag gtaaccgtcc tggcgacaac tggcagtttg acggcgatgc
    11041 ggaaactatc gcccattttg cccgtatgac taccgttttc accaccctga aaccttatct
    11101 gaaagaggcc gtcgcgctga atgcgaagtc cggtctgccg gtcatgcgcc cgctgttcct
    11161 gcattacgaa gacgatgcac atacctattc cctgaaatat cagtacctgt taggccgcga
    11221 cattctggtc gctccggtac acgaagaagg tcgcagcgac tggacgctct atctgccgga
    11281 ggataactgg gtacatgcct ggacgggtga agcgttccgg ggcggggaag ttaccgttaa
    11341 tgcgcccatc ggcaagccgc cggtctttta tcgcgccgat agcgaatggg cggcactgtt
    11401 cgcgtcgtta aacagcatct aatcacatcc gcccgtgggg aaactcatgg gcaaagggag
    11461 aacatcgatg agtcacatca caacggaaga tccagcaact ttacgcctgc cctttaaaga
    11521 gaaactctct tacggtattg gcgacctggc ctctaacatc ctgctggata tcggtacgct
    11581 ttatcttttg aagttttata ccgacgttct ggggctgcca ggcacctatg gcggcattat
    11641 ctttttgatt tcaaaattct ttactgcgtt taccgatatg ggtaccggca ttatgttgga
    11701 ttcccgacgc aagatcggtc caaaaggtaa gttccgtcct tttattctgt atgcgtcatt
    11761 cccggtcacc ttactggcga tcgccaactt tgtcggcaca ccgtttgatg tcaccggtaa
    11821 aacggtgatg gccactattc tgtttatgct ttacggactg tttttcagca tgatgaactg
    11881 ctcctacggc gcaatggtcc cggcaatcac caaaaacccc aacgaacgcg cctcactggc
    11941 ggcatggcgt cagggcggcg ctacgctggg cctgctgctg tgcacggtgg gattcgtgcc
    12001 agttatgaat cttatcgaag gtaatcagaa acttggctat atcttcgccg ccacgctgtt
    12061 ttcactgttc ggcctgctgt ttatgtggat ctgctactca ggcgtgaaag agcgttatgt
    12121 cgaaacccaa ccaaccaatc cggcgcaaaa gcctggcctg ttgcaatctt tccgcgcaat
    12181 tgccggtaac cgcccactgt tcattctgtg tattgccaac ctctgcacct taggggcgtt
    12241 taacgtcaag ctcgccattc aggtctatta cacccagtac gtacttaacg atcccatcct
    12301 gttgtcgtat atgggatttt tcagcatggg ctgtattttc atcggcgtgt tcctgatgcc
    12361 tggcgcagtc agacgttttg gtaagaagaa ggtctatatc ggcggcctgc tgatttgggt
    12421 gctgggcgat ctgctcaact atttcttcgg cggcggctcg gtcagctttg tggcgttctc
    12481 ctgcctggcg ttcttcggct cagcgcctga actgggcgct ggtttccgac accgtcgagt
    12541 acggcgagtg gcgcaccggc gtgcgttcgg aaggcacggt ctacaccggc ttcaccttct
    12601 ttcgcaaagt gtctcaggcg ctggctggtt tcttccccgg ctggatgctg acgcaaattg
    12661 gctatgtgcc gaacgtcgca caggctgacc acaccattga agggttacgc cagttgatct
    12721 tcatctaccc aagcgcactg gcggtagtca ccattgtggc gatgggttgc ttctacagcc
    12781 tgaacgagaa gatgtatgtc cgcattgtgg aagagataga agcccgtaaa cgcacggcgt
    12841 aataataata aacgacgccc tgcggggcgt tataaggagt gattatgtct gaccataatc
    12901 cactgacatt aaaactgaat ctgcgggaaa aaatcgccta tggtatgggc gacgtcggtt
    12961 cgaatttaat gctctgcatc ggtactctgt atctcctcaa actttatacc gatgaattag
    13021 ggatgcctgc ttactatggc gggattatct ttctggtcgc gaagtttttt accgcgttta
    13081 ccgatatgct caccggattt ttactcgact cgcggaaaaa tattgggcct aaaggcaaat
    13141 ttcgtccctt tattttatat gcagcagttc cggcggcgtt aattgcaacg ctccagttta
    13201 tcgccaccac cttttgtttg ccggttaaaa cgacgattgc caccacgctg tttatgatgt
    13261 ttggactttc atacagtctg atgaactgct cgtatggtgc gatgatcccg gcaattacca
    13321 aaaacccgaa tgagcgcgcg cagctcgcgg cttaccgcca gggtggtgca accatagggc
    13381 tattgatttg taccgtggcg tttattccgt tgcagtcgct tttttctgac tcaaccgtcg
    13441 gttatgcctg tgcggcactt atgttctcca ttggcggctt tatttttatg atgctgtgct
    13501 acagaggcgt caaagagcat tatgtggaca cagcgccaac cggacataaa gccagtattc
    13561 tcaaatcttt ttgcgcgata tttcgtaatc caccattgct ggttttatgc attgctaacc
    13621 tgtgtactct ggcggcattt aatatcaaac tggcgattca ggtctattac acccagtatg
    13681 tgctgaatga tattaattta ttgtcgtgga tgggattctt cagtatggga tgcatcctcg
    13741 tcggcgtatt actggtgcca gtaactgtaa aatgttttgg taaaaaacag gtttatttag
    13801 ctggcatggt gctatgggcg gtgggtgata tactgaatta tttctgggga agtaactctt
    13861 tcactttcgt catgttctct tgtgtcgcct tttttggcac ggcctttgta aacagcctga
    13921 actgggcact ggtaccagat accgtagatt acggtgaatg gaaaaccggt attcgtgccg
    13981 aaggttctgt ttataccggt tataccttct ttcgtaaaat ttctgccgca cttgctggct
    14041 tcttgccagg cattatgctg acgcaaattg gttatgttcc caacatcgcg caaagcgatg
    14101 cgacattaca gggtttgcgt cagctcattt ttatctggcc ttgtgcactg gcaattattg
    14161 ctgcattgac gatgggattc ttttacacac tcaatgaaaa acggtttgca ttgattattg
    14221 aggaaatcaa ccaacgcaaa aataaagaga tggcgacaga aaaaaaaacg gcttccgtaa
    14281 cgttataaat gtcaatagcc gctatttcca tcctggtggg tggcggcctc cctacgttta
    14341 aaaaatggac ttattatgaa aaagattaat gcaataattt tattatcttc tctgacctct
    14401 gcctcggtat ttgctggcgc atacgtcgaa aacagggagg catataatct tgcctccgat
    14461 cagggcgagg ttatgctacg tgtgggttat aacttcgata tgggcgcggg tattatgtta
    14521 accaatacct acacctttca gcgagaagat gaactaaaac atggctataa tgaaattgaa
    14581 ggttggtatc ctttattcaa accaaccgat aaattaacca tccagcccgg tggcttaatt
    14641 aatgataaga gtatcagttc gggtggtgca gtgtatctgg ac
//
LOCUS       DAZSIO010000098        14653 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZSIO010000098 DAZSIO010000000
VERSION     DAZSIO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.098"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            81..350
                     /locus_tag="ACHX5T_002687"
     CDS             81..350
                     /locus_tag="ACHX5T_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFR8467832.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="ACHX5T_002688"
     CDS             344..709
                     /locus_tag="ACHX5T_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR8467833.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="ACHX5T_002689"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="ACHX5T_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR8467834.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="ACHX5T_002690"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="ACHX5T_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR8467835.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="ACHX5T_002691"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="ACHX5T_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR8467836.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="ACHX5T_002692"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="ACHX5T_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR8467837.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="ACHX5T_002693"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="ACHX5T_002693"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR8467838.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="ACHX5T_002694"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="ACHX5T_002694"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR8467839.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12379
                     /gene="malT"
                     /locus_tag="ACHX5T_002695"
                     /pseudo
     CDS             9674..12379
                     /gene="malT"
                     /locus_tag="ACHX5T_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="ACHX5T_002696"
     CDS             complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="ACHX5T_002696"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR8467840.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13442..>14653)
                     /gene="rtcB"
                     /locus_tag="ACHX5T_002697"
     CDS             complement(13442..>14653)
                     /gene="rtcB"
                     /locus_tag="ACHX5T_002697"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR8467841.1"
                     /translation="LTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIAVMPDV
                     HLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQAIETAV
                     PHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHLGTLGT
                     GNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRDLAYFM
                     EGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEEINCHH
                     NYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCSCSHGA
                     GRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSDLVEVI
                     YTLRQVVCVKG"
BASE COUNT         3406 a         4081 c         3653 g         3513 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgtat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtc cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cacttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggtt acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    10741 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    10801 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    10861 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    10921 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    10981 acatgaaatc aaggacatca gagaaggcac catgcatgca gaatttaacg ctctgcgcgc
    11041 ccaggtggcg attaacgatg gtaatcagga tgaagcggaa cggctggcaa aactggcact
    11101 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    11161 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctagtgcagc aaaccgaaca
    11221 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaattc agcaaagtga
    11281 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca
    11341 gctgatcaac gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcatccg
    11401 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    11461 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    11521 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagttga accgtctgga
    11581 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    11641 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    11701 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    11761 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    11821 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    11881 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    11941 actggcgaat cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca
    12001 acaactgcgc cagctgattc aacttaatac gctgccggaa ctggaacagc atcgcgcgca
    12061 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    12121 tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctaatccgca ccagcccgtt
    12181 gacccaacgt gaatggcagg tcctggggct gatctactcc ggttacagca atgaacaaat
    12241 tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    12301 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    12361 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    12421 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    12481 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccgtaa
    12541 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    12601 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    12661 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    12721 cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt
    12781 catgcaggga aaaaatcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca
    12841 caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    12901 aggtgctaaa cgatgccacg ggcgagactt ccgttgccac cacaccgcct ccggcaggat
    12961 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    13021 ccagcacccg gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg ccgccgctca
    13081 cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca
    13141 gcgtacaact tccggcgcta ccgatagcaa agcggtaatc gccgccgcgc acggtgccag
    13201 gccggaagac cagacgctgc gatcccagct ccgcgccttc caccgttgcc ctacaaattt
    13261 ccgcagccgc ttttaccgcg gtcagatgct ggcgcagcag ccccggtttc gcccgtccgg
    13321 ctcgaatgcc agtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    13381 gcaggatctg cccgccgcct tcgccctgtg cgccatccag cgcaatcatc cttttcatca
    13441 tttatccttt tacgcacacc acctgacgca gggtgtagat aacttccacc agatcgcttt
    13501 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    13561 catctttacg gcattccaca tgcgcggtgg cacgaatttg gtcttccacg ctgaacagtt
    13621 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    13681 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    13741 catattgacc agcacgcgca gacaccgcac ctttacgcgt cacgtagatc tcttcaccaa
    13801 agagctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    13861 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccaca ttttccatca
    13921 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt aggtaatcat
    13981 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagtt
    14041 ggtcctgcat ctctttttgc gccagatcga taaagtaagt cccgatggcg ttaccaattc
    14101 cgcgtgaacc ggagtgcagc ataatccaca cctgctccga ctcatcaagg cagatttcaa
    14161 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
    14221 gcggatattt ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga
    14281 cgttaaaagg cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc
    14341 cgtgtggtac ggccgtttca atcgcttgac gcagctccgc caggttttca ggcaggtctg
    14401 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    14461 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    14521 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    14581 gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
    14641 tttcagtggt cag
//
LOCUS       DAZSIO010000099        14481 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZSIO010000099 DAZSIO010000000
VERSION     DAZSIO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.099"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..118
                     /locus_tag="ACHX5T_002698"
     CDS             <1..118
                     /locus_tag="ACHX5T_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8467842.1"
                     /translation="NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(115..381)
                     /locus_tag="ACHX5T_002699"
     CDS             complement(115..381)
                     /locus_tag="ACHX5T_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR8467843.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            655..4179
                     /gene="nifJ"
                     /locus_tag="ACHX5T_002700"
     CDS             655..4179
                     /gene="nifJ"
                     /locus_tag="ACHX5T_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR8467844.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4546..5675
                     /gene="ompN"
                     /locus_tag="ACHX5T_002701"
                     /pseudo
     CDS             4546..5675
                     /gene="ompN"
                     /locus_tag="ACHX5T_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5816..6250
                     /gene="uspF"
                     /locus_tag="ACHX5T_002702"
     CDS             5816..6250
                     /gene="uspF"
                     /locus_tag="ACHX5T_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR8467845.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6426..7361
                     /gene="ttcA"
                     /locus_tag="ACHX5T_002703"
     CDS             6426..7361
                     /gene="ttcA"
                     /locus_tag="ACHX5T_002703"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR8467846.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7490..8857)
                     /gene="dbpA"
                     /locus_tag="ACHX5T_002704"
     CDS             complement(7490..8857)
                     /gene="dbpA"
                     /locus_tag="ACHX5T_002704"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR8467847.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(8887..9060)
                     /gene="ynaL"
                     /locus_tag="ACHX5T_002705"
     CDS             complement(8887..9060)
                     /gene="ynaL"
                     /locus_tag="ACHX5T_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR8467848.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(9335..10318)
                     /gene="zntB"
                     /locus_tag="ACHX5T_002706"
     CDS             complement(9335..10318)
                     /gene="zntB"
                     /locus_tag="ACHX5T_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR8467849.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(10332..10379)
                     /gene="ydaN"
                     /locus_tag="ACHX5T_002707"
     CDS             complement(10332..10379)
                     /gene="ydaN"
                     /locus_tag="ACHX5T_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR8467850.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            10573..11805
                     /gene="dgcM"
                     /locus_tag="ACHX5T_002708"
                     /pseudo
     CDS             10573..11805
                     /gene="dgcM"
                     /locus_tag="ACHX5T_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11826..12389)
                     /gene="smrA"
                     /locus_tag="ACHX5T_002709"
                     /pseudo
     CDS             complement(11826..12389)
                     /gene="smrA"
                     /locus_tag="ACHX5T_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12643..14097)
                     /locus_tag="ACHX5T_002710"
     CDS             complement(12643..14097)
                     /locus_tag="ACHX5T_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HFR8467851.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14094..>14481)
                     /locus_tag="ACHX5T_002711"
     CDS             complement(14094..>14481)
                     /locus_tag="ACHX5T_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR8467852.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3861 a         3651 c         3418 g         3551 t
ORIGIN      
        1 taacaccatt agcgaaatgc tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt
       61 aacgctggcg accgcgaaac agacattaac ttataagctg gcggatttaa tgaattaata
      121 gctgccacag ctcccggcgg caagtgactg ttcgctacag cgtttgccgt tgggtaatgc
      181 acacatccca atcgccgtac catccagttg acgggcaaca gaaagcgaac cgccgatcat
      241 tgcacaattt gcttctccac tactggacat cgacgctttt aaacttggcg ctacgtgcgc
      301 cgcagtcgcc tgctgaacag gctcactact acacgccgac aacaataaag cggcacaccc
      361 tacccaaaac gctgctcgca tctcttcttc ctctgatctt caagccaaac gacaccgcca
      421 taaataatag gcagcacaga gggcggcgtc gagagctgtc ctgcgcgttg ccccgcattt
      481 ttactttttt atggctattt ttttgccctc tgtttgatca aaacattcat tactctgatg
      541 tgggggacac aaaagcgaaa atgcagaaga aagccatttg ctaaaattga aggattacta
      601 ctgggcgcgc agcaatttcg tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt
      661 actattgacg gtaatggcgc ggttgcttcg gtgacgtttc gcatcagtga agttatcgcc
      721 atctacccta ttacccccag ttccacgatg gcagaacagg ctgatgcctg ggccggaaac
      781 ggcttgaaga acgtttgggg agacacacca cgcgtggttg aaatgcagtc ggaagcgggt
      841 gctatcgcta ccgtgcatgg cgctttgcag acgggtgccc tttcaacatc gtttacgtca
      901 tcgcagggtt tgctgctgat gatcccgacg ctgtacaaac tggcaggcga actgacgcca
      961 tttgtcctgc atgtagcggc acgtaccgtt gccacacatg cactttctat ttttggcgat
     1021 cattccgacg ttatggcggt gcgccagacg ggttgcgcga tgttgtgtgc ggctaacgtc
     1081 caggaagcgc aagactttgc tctcatttcg cacatcgcaa cgctgaagag ccgcgtgcca
     1141 tttattcatt tctttgatgg ttttcgcacg tcccacgaaa tcaataaaat tgtcccgctg
     1201 gccgatgaca ctattcttga tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg
     1261 gcgctcaacc cggaacatcc ggtgatccgc ggtacgtccg ccaatcctga cacttatttc
     1321 cagtctcgcg aagccaccaa tccctggtac aacgcggtct atgaccatgt tgaacaggcg
     1381 atgaatgatt tcgctgccgc gacaggtcgt cagtatcagc cgtttgagta ttacgggcat
     1441 ccgcaagcgg aacgggtgat tatcctgatg ggctctgcca ttggcacctg tgaagaagtg
     1501 gttgatgaat tgctaacccg tggcgaaaaa gttggcgtgc tgaaagttcg tctgtaccgt
     1561 cccttctccg ccaaacactt gctgcaagct ctgccgggat ccgtacgcag cgtggcggta
     1621 ctggacagaa ccaaagaacc cggtgcccag gcagaaccgc tctatctgga tgtgatgacc
     1681 gcactggcag aagcctttaa taatggtgag cgcgaaaccc tgccccgtgt cattggtggg
     1741 cgctatggtc tttcatccaa agaatttggc ccggactgtg tactggcggt atttgccgag
     1801 ctcaacgcgg ctaaacctaa agcacgcttt acggttggta tttacgatga tgtgaccaat
     1861 ctgtcactgc cgttgccgga aaacactctg ccaaactcgg cgaaacttga agccttgttt
     1921 tatggccttg gtagtgatgg cagcgtttcc gcaaccaaaa acaatatcaa gattatcggt
     1981 aattccacgc cgtggtacgc acagggctat tttgtttacg actccaaaaa agcgggcggc
     2041 ctgacggttt ctcaccttcg cgtgagcgaa cagccgattc gttccgctta tctcatttcc
     2101 caggctgatt ttgttggctg ccaccagttg cagtttatcg ataaatatca gatggctgag
     2161 cgtttaaaac ctagcggcat tttcctgctt aatacgccgt acagcgcaga tgaagtgtgg
     2221 tcgcgcttgc cgcaggaagt tcaggccgtg ttaaaccaga aaaaagcgcg cttctatgtg
     2281 attaacgcgg cgaaaatcgc ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg
     2341 cagatggctt ttttccatct gacgcaaatt ctgcctggcg atagcgccct cgcagaattg
     2401 cagggtgcga ttgccaaaag ttacagtagc aaaggtcagg atctggtgga gcgcaactgg
     2461 caggctctgg cgctggcgcg tgaatccgta gaagaagttc cgttgcaacc ggtaaatccg
     2521 cacagcgcca atcgaccgcc agtggtttcc gatgccgccc ctgatttcgt gaaaaccgta
     2581 accgcggcga tgctcgccgg gcttggtgat gccctccccg tttcggcgct gccgccagac
     2641 ggcacctggc cgatgggcac cactcgctgg gaaaaacgca atatcgccga agagatcccc
     2701 atctggaaag aggaactctg tacccaatgt aaccactgcg tcgccgcttg cccacactca
     2761 gctattcgcg caaaagtggt accgcctgaa gcgatggaaa acgcccctgc cagcctgcat
     2821 tcgctagatg tgaaatcgcg tgatatgcgc gggcagaaat atgtcttgca ggtggcaccg
     2881 gaagattgca ccggttgtaa cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat
     2941 ccagagatta aagccatcaa tatgatgtct cgccaggaac atgtcgaaga agagaaaatc
     3001 aattacgatt tcttcctcaa cctgccagaa atcgaccgta gcaaactgga acgtattgat
     3061 attcgtacat cgcagctgat tacaccgctg tttgaatatt caggtgcttg ccccggttgt
     3121 ggcgagacgc cgtatattaa attgctgact cagctctatg gtgaccggat gttgatcgct
     3181 aacgccaccg gctgttcttc aatttatggc ggtaacctgc cctctacacc gtataccacc
     3241 gatgccaacg gtcgtgggcc ggcatgggcg aactctctat ttgaagataa tgccgaattt
     3301 ggccttggtt tccgcctgac ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat
     3361 caatttgccg ataaaatccc ggcggaatta ctgacggcgt tgaaatcaga cgctacgcca
     3421 gaggttcgtc gagaacaggt cgcagcttta cgccagcaac tcaacgatgt tgccgaagca
     3481 catgaacttc tgcgtgacgc agatgcactg gtggaaaaat caatctggct gattggtggc
     3541 gacggctggg cttacgatat cggctttggc ggtctggatc atgtattgag tttgacggaa
     3601 aacgtcaaca ttctggtgct ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg
     3661 aaagcgacac cgctgggtgc agtaactaag ttcggcgagc acggcaaacg taaagcgcgt
     3721 aaagatcttg gcgtcagtat gatgatgtac ggtcatgttt atgtggcgca gatttctctc
     3781 ggcgcgcagc tgaaccagac ggtgaaagcg attcaggaag cggaagcgta tccgggacca
     3841 tcgctgatca ttgcttatag cccgtgtgaa gagcatggtt acgatctggc tctcagccac
     3901 gaccagatgc gccaactcac tgctaccggc ttctggccgc tatatcgctt tgatccgcgt
     3961 cgtgccgatg aaggcaaact gccgctggcg ctggattcac gcccgccgtc agaagcactg
     4021 gaagaaacgt tacttcacga gcagcgtttc cgtcgtctga attcgcagca gccagaagtg
     4081 gcagaacagt tatggaaaga tgctgcagct aatttgcaaa aacgctatga cttcctagca
     4141 caaatggccg gaaaagcgga aaaaagcaac accgattaaa tgctctggat aaggattatc
     4201 caattctaaa aaaaaagccc ggacgactgt tcgggcttgt ctttttatat gttggaaaat
     4261 cagtggcaat gcaatggccc aacagaaaat aaaatctgtg acaataaagg catataaccc
     4321 gcgcagaata acgtatagga aattaaaagt ataattttta ttttgtatgt attatcttcg
     4381 atacattcac cagacttatt atcatttcag aggaattatt tgattaaggt tttacttaag
     4441 gcgtaacaaa tgatatttat cggctaactg aacttctcct ttaggatgtt ttcactcccc
     4501 gaaatgggga taaaaggcaa ataaaataac taaaggattt attcaatgaa aagaaaagta
     4561 ctggcacttt taattcctgc cctgctcgcc gcaggtgctg cacatgcagc cgaagtttat
     4621 aataaagacg gcaacaaatt agatctgtat ggcaaagttg atggcctgca ttatttttct
     4681 gataattcag cgaaagatgg cgaccagagc tatgcgcgtc tgggttttaa aggcgaaacc
     4741 caaattaacg atcaacttac tggctacggt caatgggaat acaatattca ggcaaacaac
     4801 actgaatctt caaaaaacca gtcatggacc cgtctggcat ttgccggact gaaatttgca
     4861 gattacggtt ctttcgatta cggacgtaat tatggcgtaa tgtacgacat cgaaggctgg
     4921 accgatatgc tgcctgaatt tggcggtgac tcttatacca atgcagacaa ctttatgact
     4981 ggtcgagcca atggcgtcgc gacttatcgt aatactgatt tcttcggtct ggtaaatggt
     5041 ctgaacttcg cggtgcagta tcaaggtaac aacgaaggag ccagtaatgg tcaggaaggc
     5101 accaataacg aacgcgatgt tcgtcatgaa aacggtgacg gctggggtct ttccacaaca
     5161 tatgatttag gcatgggctt tagcgctggt gcggcataca cctcttctga ccgcaccaat
     5221 gaccaggtta accatactgc ggcgggtggt gataaagcag acgcgtggac tgctgggcta
     5281 aaatacgatg ctaacaatat ttacctagca accatgtatt cagaaacgcg taatatgacc
     5341 ccgtttggcg acagcgatta tgcagttgcg aacaaaaccc agaactttga agttactagt
     5401 accagtttga tttttgtctg cgtccggcag tctctttcct gatgtctaaa ggccgtgacc
     5461 ttcacgctgc tggtggtgca gacaacccgg caggtgttga tgataaagat ctggttaaat
     5521 acgccgatgt tggcgcgact tactatttca ataaaaacat gtccacctat gttgactata
     5581 aaatcaacct gttggatgaa gatgacagtt tctacgctgc caacggcatc tctaccgatg
     5641 atattgtcgc tttaggtctg gtttatcagt tctaaatcct cctgcccgct gttatggcgg
     5701 gctttttctg cttattcttc ctcctttgat ctaaattaaa aatgtgaact ccgtcattac
     5761 acaaaaagtg tcatctggcg ttacacttta tgcggatact aaaacaggag gttttatgaa
     5821 cagaacgatt cttgtcccta tcgatatttc agattcagaa ttaactcaac gcgtgattag
     5881 ccacgttgag gcagaggcaa agattgatga tgcagaggtt catttcctga cggtaatacc
     5941 ttcactgccc tactatgcct ctctgggttt agcgtattcc gcagaattac cggcaatgga
     6001 tgacctgaaa gcggaagcca aatcgcaact ggaagagatc attaaaaaat ttaaactgcc
     6061 aaccgacaga gtgcatgtcc atgttgagga aggctcgccc aaagaccgca ttctggaatt
     6121 ggcgaagaag atccccgctc atatgatcat cattgcttcc catcgaccgg atatcaccac
     6181 ttatctgctc ggttccaacg ccgcagctgt agtgcgtcac gcagagtgct ccgtgctggt
     6241 tgtgcgctga cactaacgcc cgcacattgc tgcgggcttt ttgattcatt tcgcaaatgt
     6301 gctgacattt tcccctctaa tccgtaccat acacgccaca gtttttatat cagatttctt
     6361 atgctgagcg ttccggcatg acgcatactc ttctgatgcc atataacgaa ttgagtcgct
     6421 tttaaatgtc gcaaaatcaa gaaattagta agaaagaaca atacaacctg aacaaattac
     6481 aaaaacgtct gcgtcgtaac gtgggcgaag ccattgctga cttcaatatg attgaagaag
     6541 gcgatcgcat catggtttgc ctctccgggg gtaaagacag ctataccatg ctggagattc
     6601 tgcgcaattt gcagcaaagc gcgccaatca atttttcgct ggtggctgtt aacctcgatc
     6661 aaaagcaacc gggcttcccg gaacacgttc tgcccgagta tcttgaaaag ctgggcgttg
     6721 agtacaagat tgttgaagag aacacttacg gtatcgtgaa agagaagatt ccagagggca
     6781 aaaccacttg ctcactgtgt tctcggctac gtcgcggtat cctttatcgc accgcaacgg
     6841 aactggggac gacgaagatc gcgttgggtc accatcgtga cgatatcctg caaacgttgt
     6901 tcttaaatat gttctacggc ggcaagatga aaggtatgcc tccgaaactg atgagcgatg
     6961 atggcaaaca tatcgttatt cgtccactgg cctactgccg cgagaaagat attcagcgat
     7021 ttgccgatgc aaaagcgttc ccgattattc cgtgcaacct gtgcggttca cagcctaacc
     7081 tgcaacgtca ggtgattgct gacatgttgc gtgactggga taaacgttat ccagggcgta
     7141 tcgagacgat gttcagcgcg atgcagaatg tggtgccgtc gcatctgtgc gataccaacc
     7201 tgttcgattt caaaggcatt acccacggtt ctgaagtggt taacgggggt gatctggcgt
     7261 ttgatcgcga agagatccca ctacaaccgg cgggctggca gccagaagaa gatgaaaatc
     7321 agttggatga gttacggctg aatgtggttg aagtgaaata accaggatag cgcccgatgc
     7381 gcaagcgtat cgggctactc ttatggaggc cggataagac gcggccagcg tcgcatctgg
     7441 caatcccgaa taagatgttt actcttgcac ccggcaattc aacatttcat tattttaata
     7501 accgcacccg gcacgttttt cctttaatct tcccgccctg taactgtttc catgctttat
     7561 gagcaacagc ctgacggacc gcgacataga catgcgccgg atgcacggcg attttgccaa
     7621 tatctgcgcc atcaagcccg atatctcctg tcagtgcacc taatacatca cccgggcgca
     7681 ttttggcttt tttcccgcca tcgatacaca acgttgccat ttctgcttcc agcgtcacaa
     7741 tggaactatt agctggcggc gtttgccagt taagttttat ctgcaacatg tcagaaatga
     7801 tattggcccg ctgtgcttct tccggagcac agaaactgat cgccagaccg ctatttcctg
     7861 cacgagctgt acgaccgatg cgatgtacat gaacttcagg gtcccacgcc agctcaaagt
     7921 tcaccaccag ctcaagcgat ttaatatcca gaccacgcgc agcaacatca gtcgcaacca
     7981 gtacacgggc gctaccgtta gcaaaacgta ccagggtctg atcgcgttgc tccagatcgc
     8041 cgtgtaacga caatgcactt tgctctactt cattcagcgc gtcgcagaca gcctggcaat
     8101 cttttttggt attgcaaaac accacacaag aggatggctg atgcaagctt aataaccgtt
     8161 gcaacagagg aattttgcct ttactggatg tctcataaaa ttgttgttca atgggtggca
     8221 aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg ttgcactcgt ccgctgattg
     8281 cggcgatggc ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa
     8341 aacggatgac atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat
     8401 ccatcaccaa agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca
     8461 aacgacccgg cgttgccacg ataatatgcg gcgcatgttg cagcgaatca cgctgcatac
     8521 cgaacggttg accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc
     8581 gacgcaattc acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct
     8641 gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt
     8701 tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg
     8761 cggcggcctg caccggcgtc atggttaaat aacccaactc attaaggttc gtgagttggg
     8821 cgggaggcaa aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac
     8881 gcaaagttag caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg
     8941 ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg
     9001 aacaggatcg ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat
     9061 atttccctcc ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa
     9121 aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa
     9181 aaaaggaagt aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca
     9241 agggagatat tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt
     9301 ttttgtatac agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc
     9361 ataaagcaac accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt
     9421 gccacccgcc gccagggatc ccaccaaggt tgacgccaaa taacccggtc agaaaggtac
     9481 tgggtaaaaa gaccattgcc atcaacgaca ttgtataggt acgacgagct aaattttcct
     9541 gcatcacctg agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt
     9601 cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc
     9661 tcatccacgg caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat
     9721 agcgacgcat cacaattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt
     9781 gctgatcaag gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt
     9841 cactggaatg atcggtcaac gcatcgcaca catccaccag ccatcccccg caatcggtcg
     9901 gacccgtgcc ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc
     9961 gggtcgaaac aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc
    10021 gttcatcggt gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgaaacggc
    10081 tgactcgggg ccgtgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg
    10141 gtgttgtcgc cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg
    10201 gatgcgcttc atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca
    10261 gcatccaggc aaatactgca tccgggacat taacgtccga tcccttaatc gcttccactg
    10321 tgcctccatc atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc
    10381 tcatcacccc attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc
    10441 aaaatgcttt tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa
    10501 ttatctgatc atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat
    10561 aacgggatct caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc
    10621 tggattgttt cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg
    10681 atgcaagacc tcacgtttag tcagctacga accggaccct attccgtctc ctcacaaaaa
    10741 gaactgccga aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact
    10801 gttcagcgta aagaagagga aacagcatta agctgtcggc ttgttttgcg agagctgaca
    10861 gaaacagaac cggtgattat tttcgaaggt atcgaagcgc cggcaacgct gggtttaaaa
    10921 gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg
    10981 actaactctg caccaatgtt gttgattgac ccgtcacgag atggacaaat cgtcgatgct
    11041 aacctcgccg cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc
    11101 taggaaataa atatgcttgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta
    11161 cccggcggtc ataacccttt gaattttgtt cataaactgg cggatggatc tactcgtcat
    11221 gtgcagacct atgccggacc gattgaaatt tatggcgaca agctcatgtt atgtattgtg
    11281 catgatatta ctgagcaaaa acggctggag gagcagctgg aacatgctgc tcaccatgac
    11341 gcgatgaccg gattactgaa tcggcgacag ttttatcaca ttacggaacc aggccaaatg
    11401 cagcatctcg ccatcgctca ggattacagc ttgttgctca tcgacaccga tcgttttaaa
    11461 cacattaacg atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc
    11521 cccctcgaaa gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt
    11581 gtcttattgc taccaagaac cccactggat accgcgcttt cgctggctga aactatccgc
    11641 gtaagcgtgg caaaagtgag tatttcgggc ttaccacgct ttaccgtcag cattggtgtg
    11701 gcgcatcacg aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg
    11761 tatcgggcga aaaatgatgg acgcaaccgc gtgctggcgg cataagccgc ggatgcgtct
    11821 cgagatcaac gactgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt
    11881 ttacgtagtg cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc ggtgcaatat
    11941 gcctgaacat catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca
    12001 tgtgatttat cttcccgccc tttaccatga ataatcagca cattacgcaa accatccgcc
    12061 agggcttgtt gaatgaaact gaacaccatt ttgcggcact cttccaccgg ctggcgcaaa
    12121 agattcaggc tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgc
    12181 tacaaccctt cctgccgaaa ctccagcggc tgacttagcg ggatgatgtc gagaaatccg
    12241 gtagtgagga aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc
    12301 gttggatgcc agtggacatc tgtagcacgt ttcagcggct ggacatcttc catggcgtca
    12361 agaaacagcg atttgtcgtc aaggttcatg taacctccta tgcaccttca cacgtcgtga
    12421 ttcagacggg ttaattgttc atacttactg catcgttccc ttcacagatt gaaacaatac
    12481 tctacttcat acctgtgagt atctgcgcag gcggggcatt ttatactagc ctgttagcag
    12541 tatcgaacag tttagcccga gtttaattca gcacaggctc cgatcaggat gatttattca
    12601 tagcaggaac caacaaaaat gcgataaata acagaccact gattagcaga aaggtaccga
    12661 aaatgccata gtttgcgcct ccctcagcat caattgactc aataatttgg ttgaagccag
    12721 tatttccgat gtctggtcac ttctactgcg attaaaaaat aaagcacttc tggaaatgaa
    12781 gtcattcacc gtatggggaa cagaaaccat tttttcaccc attctcgaaa aatgaaaatt
    12841 agttcgatta ttgagaatag agccacacag ggcaatacca atcgcgcccc caagattacg
    12901 tgttaaatta aatacaccag aggctaactt cagccgttct tttggaattc cccccagcgt
    12961 taatgttacg atgggggcca tcgcaaactg ctgcgaaatc cctctaattg cctgtggaaa
    13021 cagtaattcc tgccatcccc attcgtgggt tataggggta aaaagataca ttgaaaacac
    13081 aaatccgccc agccccgcca tcaacagcca tcgcagattt atttttttac ttaaccagaa
    13141 gtaaaaggga acagaaaata actggaaaat acctgttgtg caaacggcaa aacctatctc
    13201 ttcggcattt aatccgcgaa tctgccccaa aaacacaggt attaaatata cagtagagaa
    13261 tatcccaacc cctccagaaa aagaaaaaaa acatcccaga gtgaaatttt tatccttaaa
    13321 agcatgaaga tccattatcg gattggatat tgtaagtgta cgcgcagcaa ataaaataaa
    13381 actcaccaat gccagaactg atgtcagcaa aatagtatta tcatccagcc atccccagcg
    13441 cgctccttct tcaagggtat attccagaca tcccagagtc atggccaaca ggataatact
    13501 cggataatca gcaactttca gtaaagagag atcaggttta tcaaaattaa ccagaaaagg
    13561 aatactcaaa actaaatata tgccaggtaa tatattgata taaaatagcc atcgccagtc
    13621 gaggttatca gtgagccagc cccccagagt tggccctagc gtcggtgaca gtgaggccag
    13681 cgcactgact accgcagcag ccaaccccaa ctctttaccc tggtaataaa taaacgccat
    13741 cgtgaaaact aaagggatca tggatgcgcc agcagctccc tgtaaagcac gaaacaaaat
    13801 catgatctga atattccatg caagtccaca cgcgatactc attaaagtaa atattcccgc
    13861 ggatagagta aaaagccaac gagtggaaaa tacccgtgac agccacccag agagcggaat
    13921 aacaataatc tcagcaatta aatagctggt ctgaagccag gccatttcgt ctttacctgc
    13981 ggacagacca ccacctattt catttaggga tgacgataca atttgaatat caagctgagc
    14041 aataaataat cccatacaca tactggcgaa agcaatatgt ttacgcatag acatcatctc
    14101 tcatcaacac tgacggtgac ggacagtccc gggcgaagaa gttgctttaa ttcctttgcg
    14161 tcgtcaaatg caatccgtac cgggacacgc tgaacaattt tggtgaaatt gccagtggca
    14221 ttttctatcg gaatcaagct gaaactggct cctgtcgctg gggaaaggct ttcaatgtga
    14281 ccgtgaaaca cttccccttt gagaatatca gcacggatct cagccttcat tcctggcttc
    14341 attcccgcta tttggttttc tttataattt gcatcaaccc acaactcact gactggtact
    14401 agtgaaacta acgatgtgcc gccttccacc caactgcctg tatgggcgct gcggtttgcg
    14461 acaatgccat caatcggcgc a
//
LOCUS       DAZSIO010000100        14436 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZSIO010000100 DAZSIO010000000
VERSION     DAZSIO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.100"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..185
                     /locus_tag="ACHX5T_002712"
     CDS             <1..185
                     /locus_tag="ACHX5T_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR8467853.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
     gene            complement(210..401)
                     /locus_tag="ACHX5T_002713"
     CDS             complement(210..401)
                     /locus_tag="ACHX5T_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR8467854.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(506..742)
                     /locus_tag="ACHX5T_002714"
     CDS             complement(506..742)
                     /locus_tag="ACHX5T_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFR8467855.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="ACHX5T_002715"
                     /pseudo
     CDS             complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="ACHX5T_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="ACHX5T_002716"
                     /pseudo
     CDS             complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="ACHX5T_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="ACHX5T_002717"
     CDS             complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="ACHX5T_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR8467856.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6381..6878
                     /gene="msrC"
                     /locus_tag="ACHX5T_002718"
     CDS             6381..6878
                     /gene="msrC"
                     /locus_tag="ACHX5T_002718"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR8467857.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6975..7673
                     /gene="proQ"
                     /locus_tag="ACHX5T_002719"
     CDS             6975..7673
                     /gene="proQ"
                     /locus_tag="ACHX5T_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR8467858.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7693..9741
                     /gene="prc"
                     /locus_tag="ACHX5T_002720"
     CDS             7693..9741
                     /gene="prc"
                     /locus_tag="ACHX5T_002720"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR8467859.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9933..10814
                     /gene="htpX"
                     /locus_tag="ACHX5T_002721"
     CDS             9933..10814
                     /gene="htpX"
                     /locus_tag="ACHX5T_002721"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR8467860.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMMMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10860..12233)
                     /locus_tag="ACHX5T_002722"
     CDS             complement(10860..12233)
                     /locus_tag="ACHX5T_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8467861.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12410..13201
                     /gene="kdgR"
                     /locus_tag="ACHX5T_002723"
     CDS             12410..13201
                     /gene="kdgR"
                     /locus_tag="ACHX5T_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR8467862.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13344..13583)
                     /locus_tag="ACHX5T_002724"
     CDS             complement(13344..13583)
                     /locus_tag="ACHX5T_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR8467863.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13742..13885
                     /gene="mgrB"
                     /locus_tag="ACHX5T_002725"
     CDS             13742..13885
                     /gene="mgrB"
                     /locus_tag="ACHX5T_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR8467864.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13960..14247
                     /locus_tag="ACHX5T_002726"
     CDS             13960..14247
                     /locus_tag="ACHX5T_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR8467865.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
BASE COUNT         3792 a         3521 c         3698 g         3425 t
ORIGIN      
        1 atgaatggca aaaggacgtt gatttgcatc aggtcttgtg gagccgctcg cgattaggtg
       61 aacgcccaaa agggcaggga attacaggtg ctgatcattt ctggtttggt catacaccgt
      121 tgcgacatcg cgtggatatt ggcaacctgc attatattga taccggtgct gtctttgggg
      181 gctaactgac tcttgtgcaa ctgcaataat taaaaatcac catactcctg tgctggttgc
      241 cagaaaccat ctataaaatc ctcaatcgga aaacaactgc catggcggat ccgttgatcg
      301 ctcatagaat aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa
      361 ccgccatcca ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt
      421 gccgtaccgt taagtttagg agagatttta caacggggga ataaccagga caaataaccc
      481 gccatgccga cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt
      541 gatcgtaacg ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg
      601 tttcagcatc ccaggcatcc agttgcatac ataaatcggg gtcagattta caaggaatgg
      661 ttaacgttcg atcacccggc gattcaccgt gtaattcgcc gtcgtcgatt tcaaaagcgc
      721 ctatgcgcac actggttttc atcttcgtgc tctccattta gctgctgatt gtagtacgac
      781 cagtccgatc gcacaatttt tcagcctagt tgaggccggc gaatgtgcca gtcaaaaagt
      841 gcgctttttt tgtgcgctgt catgcgttac tggtaaaaag tttcccgtcc cgcaccagtt
      901 cacgcgggta gctgtttttc agtcgcgaac caatccgttt ggctaaacca atcggctgat
      961 gctggaaagt aaccaacacg tcatccgcca ctggcgcggc ttgcgggtaa acatcgcgcc
     1021 cgcgatacca ctcctacgct tcctgcggtg tcagttcaaa agcgtttaca ttgtcggggg
     1081 aggcatgggc aataaccgct tcatgctgcc agcgataacc tttgttatgc gtttcggcaa
     1141 gtttaatccc taagcgagaa aatcggactt taccgatcag ggcttcaatg cctaccggga
     1201 acaaccacag ttctttgtcg cgttgccaga gtcgcaggtt tccatcccag tttaagccaa
     1261 cacttgcagc cgcctgacga atttgtccgg cttcgcgatc tttcaccggg ctgaacggga
     1321 aattgcccac tttgtatttt ggggcgggta aggcggggat cgcctgagtt ttacgcagac
     1381 gagcaacgaa gaagccttcg cagtcgtaaa tttgtgggaa aacatgcaaa aaaccttctt
     1441 cggtcagcgc cttgtttgca ccagggaaga gatcgccaag tggtaaaaac tctacttcgt
     1501 cggggtaagt ctctttcagc caccggcaaa cggcttcgtt ttcttcccgg tttaaggtat
     1561 aggtcgagta aaccagcgta ccgccaggac gtaatgcatg aaaggcgctg tcaatcagtt
     1621 cccgttgggt agctgcgatt tcctgattgc tttcgggtga ccagtttttt agcgcatcgg
     1681 gatctttacg caccacgcct tcgccggagc agggagcgtc cagcaaaatg gcatcaaaca
     1741 tttctggcac tgccgcacca aacacgcggc caccaaaatg tgtgagcgca acattactga
     1801 tgccacagcg gctgatattg gcatgtaaca cttttaccca actggcggaa aactcattgg
     1861 caaggattgc cccttcgtta ttcatccgcg cggcaatttg cgtcgttttt gagcctggcg
     1921 cagcagcgac atccatcacc cgctgtggtg cattaccgtc agcaaacaag gcggcgacgg
     1981 gcaacattga actggcttcc tgaatataaa acaggccact taaatgctcg gtggtactac
     2041 ccaatggcaa tgcatcttca ttgtcgcgtt caatccagaa accttcttca caccacggaa
     2101 ttggcgtaag cgtccagcca taaggagcgg ttaattgcag gaaatcagca acggagattt
     2161 tcagcgtatt aacgcgaatg ctgcggcgca acgggcgctg acaggcggca agaaaatcat
     2221 caaatgagag cgtcgaaggc atagcttcgc gcatttgtgt cagaaacgcg tccgggaaat
     2281 aaacggtgtg ttgggccacg agcatgtacc actggcaaaa aaacaggcgc gcagtttagc
     2341 ataagcgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagttccc
     2401 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgtttgcc ttcttgggct
     2461 ttcggtgcaa gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaattgg
     2521 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     2581 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     2641 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2701 agaccacgga acagcacagg cgtaccgatg cctaacgaac cggcttccgg cgcttcaaca
     2761 ataatgctta aaccatctag gtaacgcgaa tcagtaatgg tggcctcttc taattcaaag
     2821 tcgcgacgag gattgccacg gcctggttcg acgttgatat acggctggag gatagtatct
     2881 agatgctcaa cgccagctgc cgagatttgc ggtgtgacca ctgagaagcg cgtaccaccg
     2941 cgagcaaagg tctggacata ttccggatag agcaccgcct ttgcctgtac ttcattgcgt
     3001 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     3061 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgcct gccaaccgca
     3121 cgggccgctg tttcggaagc atacagaatt cgcttgtcgc ctttacgctg actggcgctg
     3181 gcaccactga ggttatcgaa gctaatggcc ccctttaatg ctctggagag cggggatgcc
     3241 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     3301 ctgttgctgg tcaaaaggtt gcgatattcc ggtttaatgt gcagatcgat atcgaacgcg
     3361 ttagctcgcg gacgcacggt aataacttca ccaacttcaa atttacggta cagcactacc
     3421 gatcctgcct gcacatccgg cagcgtctct gcactcaaac tcatggttgt ggtgggtaaa
     3481 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     3541 atctcgcctt tatcgcccgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     3601 gcaccgagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3661 aatttgctat cgccttttac cagttctcga tgctgaggct cgatggcgac ggtaaaggtg
     3721 acgcctttgc tggtgagttt acgatcgata acctggccta cctgcacgcc gtgaagaatg
     3781 agcggctgac ccgcatcaat accgtaactt tccggtgctg tcagggtcag tgtcagaacg
     3841 tccggctctt gcagcagggc tttttcgccc ggcacaacaa cgaactcttt gcgtggctcg
     3901 ccatcgccgg gcaccaactc aaaggttttt ccggtcagca gggcgctgag attagcatcg
     3961 ctaagggata atttcgggtt gcgtaattcg atgcgggtat tttcacgaag cagggatcaa
     4021 cggtcatttc cccagtgact ttaccaccag gatttaaatc cagtttagtc agctgtccga
     4081 cttccagccc ctgatacatt aacggcgtcg agtcggcggt taatcctgct ccactcggca
     4141 gttccagttt tattattact ccacgctggc tgtgggccag atcttcatac agaccaaagg
     4201 tatcttccgc ctcggcaggt ttcgactctt caggtgaatc gaaggcaatc gcaccgttaa
     4261 ccagtgccgc cagactttcc agtttcacct tcgcgccact gatactgacg ttggcatcaa
     4321 cgccggaaac gtttcagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga
     4381 tcaggacatc aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc
     4441 ccaccgggat cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agagcggggg
     4501 cttgcaggtg gatcatcaga tcgccattgt ccagccgata tttcggttgg gtatcgagcg
     4561 cgacaaagtg atcctgctct ttacctttac ccggcatcat gccgatatag ttcccaccga
     4621 cgagggcgtc cagcccggag acacctgcca acgatgcttt tggcgtcatc agccagaact
     4681 gagtctcttc gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct
     4741 tacgaagatc gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac
     4801 gaacaggcgt acggcccgga acaataccat ccgccgacat aaagtcgatg gtgacggtat
     4861 taccccggtc ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag
     4921 gcagcagcca gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag
     4981 cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca
     5041 agaatagtca aaattacggc agcgccaaaa taaaacgcag ccggtcccat agtaaaagcg
     5101 aggatctgat cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagtatc
     5161 gaccagcgtc caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc
     5221 tggcatttaa aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt
     5281 accagaatac tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac
     5341 ataattccgg aaagaatcgt atcttcctgc cggccaccat tcagataaat gatagaaata
     5401 ggcaataggt tggctggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt
     5461 tgcaaactat gacgacgacg caggcgtagc gggatatggc aacgcgggca gagaccacgc
     5521 tgatcaggat agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc
     5581 gtagcggggc gctgcggata atatcgctcc cacagctcct caacattaag atgtgacaac
     5641 gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca agccgacacc cgcctggata
     5701 tgggcataat cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc
     5761 attacccact ctttaagtcg ctcaagcatc agcagtaccg gacgtaaatt cattcccagt
     5821 cggttaccaa accataaata tgctatggag gacaccagaa tgaggggggc accaataacg
     5881 cagaaaaaga ccattgatcc cgttatcgca tcgccctgtt tggtcatttg ccagatgcct
     5941 tgcatgacgt tggcgtcaat acgaatgcct aacagccaga tatgcaacag cggttcgccc
     6001 caggcaaacg gcataagcaa cagcatggtg aaggccattg ccgccaggcg cgttagcgac
     6061 cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac aataggcact ttgatgagaa
     6121 tttatctcgg gcaggctaaa cagcatgtca cattgcgggc aacgttggta atcaccacgc
     6181 ggcagttcct cgccgattgc cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg
     6241 ttaagagcca tgacgtgcca aatgtaataa gaattaacat taatcttaac ccatgatggg
     6301 aatgatcttg agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa
     6361 gacaaccaag tgattaagta atgaacaaaa cagaatttta cgcggattta aatcgcgact
     6421 ttaacgcgct gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt
     6481 tgttatacga gcgtctcact gacgttaact gggcaggttt ttatttgctt gaggacgata
     6541 cactggtact cggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg
     6601 gggtgtgcgg cacagcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt
     6661 ttgacgggca tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg
     6721 tgaaaaatca gattattggt gttctcgaca tcgatagtac tgtcttcgct cgctttacag
     6781 acgaggacga gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga
     6841 cggattacaa aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta
     6901 ctggtagcgt cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt
     6961 aatcaggaaa tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt
     7021 ttctggccga acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa
     7081 ttggtatttt tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc
     7141 aattacgatc cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac
     7201 ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg
     7261 tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg
     7321 aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc
     7381 gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac
     7441 ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac
     7501 agcacacccc ggtttctgac atttcagctc tgactgtcgg acaagccctg aaggtgaaag
     7561 cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc
     7621 agctgaattc gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag
     7681 gccgggccag gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata
     7741 gcaggccaga ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag
     7801 gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat
     7861 tatcgccagt tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat
     7921 ctgctcgatt acagccacaa tgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag
     7981 aaaaccgagt taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac
     8041 aatctggcgc aaaagcgccg ttttgaacgt taccagtacg ctttgtcggt actggaaaag
     8101 ccgatggatt tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg
     8161 aaaaacgagg ctgagttgaa cgcgcagtgg gacagtaaag tcaaattcga cgagttaagc
     8221 ctgaagctga caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa
     8281 tttgccattc gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg
     8341 gcgtttgcgc gtgaaatcga tccgcatacc aactatcttt ccccgcgtaa taccgaacag
     8401 ttcaacactg aaatgagttt gtcactggaa ggtattggcg cagtgctgca aatggatgat
     8461 gactacaccg ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc
     8521 agcgttggtg acaaaattgt cggtgttggt caaacaggca agccgatggt tgacgtgatt
     8581 ggctggcgtc ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt
     8641 ctggaaattt tacctgctgg taaagggacc aagacccgta ctgtaacgtt gacccgtgaa
     8701 cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa
     8761 gtcggcgtgc tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa
     8821 ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc
     8881 ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt
     8941 gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg
     9001 ttctataaag gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc
     9061 tttgccgcgg caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt
     9121 aaaggcaccg ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct
     9181 gaatggccag cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc
     9241 ggcagtacgc aacgtaaagg cgtaacacca gacatcatca tgccgacggg taatgaagaa
     9301 acggaaacgg gtgagaaatt cgaagataac gcgctgccgt gggatagcat cgatgccgcg
     9361 acttatgtga aatcaggaga tttaactgcc tttgaaccgg agctgctgaa ggaacataat
     9421 gcgcgtatcg cgaaagatcc tgagttccag aacatcatga aggatatcgc gcgcttcaac
     9481 gctatgaagg acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat
     9541 aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac gctttaaacg agaaggtaaa
     9601 ccggagttga agaagctgga tgatctatcg aaagattacc aggagccgga tccttatctg
     9661 gatgagacgg tgaatatcgc actcgatctg gcgaagcttg aaaaagccag acccgcggaa
     9721 caacccgctc ccgtcaagta atatcaatca ggcacaagaa attgtgcctg attttttaac
     9781 agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa gttgtgtctt tctggtgact
     9841 tacgcactat ccagacttga aaatagtcgc gtaacccata cgatgtgggt atcgcatatt
     9901 gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa
     9961 cgaacctggc cgtaatggtc gttttcgggc tggtactgag cctgacaggg atacagtcga
    10021 gcagcgttca ggggctgatg atgatggcct tgctgttcgg ttttggtggt tccttcgttt
    10081 cgcttctgat gtccaaatgg atggcattac gatctgttgg cggggaagtg atcgagcaac
    10141 cgcgtaacga aagggaacgt tggctggtca atactgtagc aacccaggct cgtcaggcgg
    10201 ggatcgctat gccgcaagtg gctatctacc atgcgccgga catcaacgct tttgcaaccg
    10261 gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg tttgctgcag aacatgagcc
    10321 cggatgaagc cgaggcggta attgctcacg aaatcagcca catcgccaat ggtgatatgg
    10381 tcaccatgac gctgattcag ggcgtggtga acaccttcgt tatctttatt tcccgtattc
    10441 tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg
    10501 gcaacccgct gatctacttt gcggttgcaa cggttctgga actggtgttt ggtattctgg
    10561 cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt ccatgcagat gccggttcgg
    10621 caaaactggt tggtcgcgag aaaatgattg ccgcgctgca gcgcctgaaa accagctatg
    10681 aaccgcaaga agcaaccagc atgatggctt tctgcattaa cggtaagtcg aaatcgctca
    10741 gtgagttgtt catgactcac ccgccgctgg ataaacgtat tgaagctctg cgtacgggtg
    10801 aatacctgaa gtaatatgaa gtaaatacta aaaagcgcgt cctgatcgac gcgctttttt
    10861 tatgccctgg ctcgtggctg agtgatacgt aaaccactga cacaggcagc aatcactgcc
    10921 agaatagccg cagccatcag cgagacgtgc gtaccattat caccaaactg atttagcatc
    10981 agcgccacca gcgccgcgcc actactctga cccagtagac gagccgttcc taacatgcca
    11041 ctggccccac cgctacgttc gcgcggagcg gaggtaataa tggtgtggtt attgggtgac
    11101 tggaataagc caaatccagc gccacataag atcatcggcc agataatatt gatatccgca
    11161 ggtgacgcgg gcagcagaac cagggaaaaa agccccgtag ccatgatgaa caaccctaaa
    11221 gcacccagca atcctgcatg tacgcgttca atcaaatagc ctgccagagg agccatcacc
    11281 atcgttgcta acggccacgg tgtcagaagt aaacctgttt cgacttcact acgcccgagc
    11341 acggtttgca ggtaaaaggg cagggaaacc attgccagca tttgtgcgca gaaagagcaa
    11401 acagatgtgc aaatagaaag tgaaaacagc gggatacgca gtaaatccac cggtagcagc
    11461 ggtacgggaa gagaaagctg gcggcgaata aagaaaatac caacaacaac cattaccacc
    11521 agttccgcag caattaacgt cagcgattgc ccctgagcga aaccactcaa cgcagtgata
    11581 agcaggccga aggttaacgc gttcatcacg gcgctgggca ggtcgaaacg gggtttactg
    11641 gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa gcagggcgat aatacctaac
    11701 ggtacgttga ttaaaaataa ccatttccag gatgcgatgg agaggattgc tgcagcaatt
    11761 gtcggcccgg cagcagaaga gacggcaaca ataaacgagt ttatgcccat ccctctcccc
    11821 agaaaacgtt gtggatagat caggcggata agtgcggtat taacgctcat caacgctgca
    11881 ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg
    11941 cagaacagtg aagacaacag aaaaacgacc agaccgcatt tataaatacg tcgatagcca
    12001 aacatatcgc ccagaaacga aaacgagagc agggagatga caatggcgat ttgataggcg
    12061 ttcactaccc agatggaact ggctggtgtg gcatgaaggt ccgtggcaat tgttggcagg
    12121 gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa taccaatcac aatggttaat
    12181 atcgcaccgt atcgctgggg caatggcagg ccgtcggcct gaacttttgg cataggaatt
    12241 ttatatcttt ggtgaataat caacaggggt atgattctag cactgatatt cacggtggat
    12301 gtcgcagaat tgtaactaac cggcaaaaga tggattgcga ccaccctggt gcgaaattat
    12361 aataaaaaac agttctgatt tttataaaac actcgcaatg aggtgataaa tggctaacgc
    12421 agatctggat aaacagcctg attctgtatc ttccgtgcta aaagtttttg gcattttgca
    12481 ggcgctgggt gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc
    12541 aaaaagcacc gtttatcgct ttttacagac catgaaaacc ttaggttatg tggcgcagga
    12601 aggggagtcg gagaaatatt ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt
    12661 acaaaacgtc gatttaattc gtagcgcaga tatccagatg cgtgagatct cccgcctgac
    12721 caaagaaact atccacctcg gcgcactgga cgaagacagt attgtttaca ttcacaaaat
    12781 tgactctatg tacaatttgc gcatgtattc acggattggg cgtcgtaatc cgctgtacag
    12841 caccgcgatt ggtaaggtac tgctggcatg gcgcgatcgc gatgaagtga agcaaattct
    12901 tgagggcgtg gagtataaac gcagtaccga gcggaccatc accagtacag aagcgttatt
    12961 acccgttctg gaccaggtgc gcgagcaggg gtatggagaa gataacgaag agcaggaaga
    13021 agggctgcaa tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca ttgccggttt
    13081 gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat
    13141 gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt tatcacgact atccgttctg
    13201 atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt ctttatttag catgagaaaa
    13261 ataaagttga aggtgcgtta tattaaacgc gcttgctata agaatatttt actcatgagt
    13321 gagaatctgg ttatttatta cccttaacca ttatcgacca cgatattgct tttacgtaac
    13381 agtgggcaat ctgttatctc caagaaacca cttttagtgt gcaagtattg taccgtgctg
    13441 gtgcctctgg cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt
    13501 ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata
    13561 actgtgcgaa tgatgaatcg cattacaatc tcttctcttt ttatgttcgc ttaatcgtag
    13621 cggcaatatg cgctgaagca agctactcat tccgaaaaag cacgaatatc gacatagtta
    13681 ggcgctgttt aactaacgca tgctagttta atgacataag gtaggtgaag cggagattgg
    13741 agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg
    13801 ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc
    13861 cattaaccag tttatcccgt ggtgatatca ttttttcagc actagatttt cttcctctcc
    13921 agcagtggta gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca
    13981 ggcgtgatga acattgcgtc tttggttgta tcggtggtgg ttcttcttat cgggctcatc
    14041 ttgtggtttt ttatcaatcg tgccagttca cggactaacg aacagattga actgcttgag
    14101 gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac
    14161 gaaccagaga aagcagataa aaagaccatt gagagtcaaa aatcggttga tgacgaagat
    14221 attattcgcc tggtcgccga acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga
    14281 agggttactc aattcatcct gattatacgc actgtcatgg tgcaactgct tcaaattaaa
    14341 actctggtct gacttcaaat aattatcata ttatttattt gcgtaacggg atatcagaaa
    14401 aagatatttt cctcattaat ttattaggat gtttac
//
LOCUS       DAZSIO010000101        14321 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZSIO010000101 DAZSIO010000000
VERSION     DAZSIO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.101"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            274..1332
                     /gene="citC"
                     /locus_tag="ACHX5T_002727"
     CDS             274..1332
                     /gene="citC"
                     /locus_tag="ACHX5T_002727"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR8467866.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1347..1643
                     /gene="citD"
                     /locus_tag="ACHX5T_002728"
     CDS             1347..1643
                     /gene="citD"
                     /locus_tag="ACHX5T_002728"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR8467867.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1640..2548
                     /gene="citE"
                     /locus_tag="ACHX5T_002729"
     CDS             1640..2548
                     /gene="citE"
                     /locus_tag="ACHX5T_002729"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR8467868.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2559..4091
                     /gene="citF"
                     /locus_tag="ACHX5T_002730"
     CDS             2559..4091
                     /gene="citF"
                     /locus_tag="ACHX5T_002730"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFR8467869.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4095..4645
                     /gene="citX"
                     /locus_tag="ACHX5T_002731"
                     /pseudo
     CDS             4095..4645
                     /gene="citX"
                     /locus_tag="ACHX5T_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4620..5498
                     /gene="citG"
                     /locus_tag="ACHX5T_002732"
     CDS             4620..5498
                     /gene="citG"
                     /locus_tag="ACHX5T_002732"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFR8467870.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5549..7011
                     /gene="citT"
                     /locus_tag="ACHX5T_002733"
                     /pseudo
     CDS             5549..7011
                     /gene="citT"
                     /locus_tag="ACHX5T_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7125..7931
                     /gene="rna"
                     /locus_tag="ACHX5T_002734"
     CDS             7125..7931
                     /gene="rna"
                     /locus_tag="ACHX5T_002734"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR8467871.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8160..8570
                     /gene="rnk"
                     /locus_tag="ACHX5T_002735"
     CDS             8160..8570
                     /gene="rnk"
                     /locus_tag="ACHX5T_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR8467872.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8708..8797
                     /gene="yldA"
                     /locus_tag="ACHX5T_002736"
     CDS             8708..8797
                     /gene="yldA"
                     /locus_tag="ACHX5T_002736"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR8467873.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8801..10010)
                     /locus_tag="ACHX5T_002737"
                     /pseudo
     CDS             complement(8801..10010)
                     /locus_tag="ACHX5T_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
     gene            10231..10659
                     /gene="uspG"
                     /locus_tag="ACHX5T_002738"
     CDS             10231..10659
                     /gene="uspG"
                     /locus_tag="ACHX5T_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR8467874.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10780..12345)
                     /gene="ahpF"
                     /locus_tag="ACHX5T_002739"
     CDS             complement(10780..12345)
                     /gene="ahpF"
                     /locus_tag="ACHX5T_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR8467875.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12590..13153)
                     /gene="ahpC"
                     /locus_tag="ACHX5T_002740"
     CDS             complement(12590..13153)
                     /gene="ahpC"
                     /locus_tag="ACHX5T_002740"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR8467876.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13525..14271
                     /gene="dsbG"
                     /locus_tag="ACHX5T_002741"
     CDS             13525..14271
                     /gene="dsbG"
                     /locus_tag="ACHX5T_002741"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFR8467877.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3440 a         3776 c         3577 g         3528 t
ORIGIN      
        1 tcaattaaat cacttaaacc tgcgagtcat ttcgtaatta cattaattaa aaaacttaaa
       61 accattaaat aaataaaaaa ccactaactc tatgtgaaat aaatcaaaat ttcacgccga
      121 aatactcctt aggatgtata gcaaaaggag aaaaagatat acctcgatca cccccctttc
      181 tcccaagtga aaataaaagg ttatcagttt gcaacattga acaacattcg ttgcaaatcg
      241 ataacaacat gcaccttcag gatactattt attatgttcg gcaatgatat tttcacccgc
      301 gtaaaacgtt cagaaaataa aaaaatggcg gaaatcgccc aattcctgca tgaaaatgat
      361 ttgagcgttg acaccacagt cgaagtattt attaccgtaa cccgcgatga aaagcttatc
      421 gcgtgcggtg gaattgccgg aaatattatt aaatgcgttg ctatcagtga atccgttcgc
      481 ggtgaaggac tggcgctgac attagccact gaactgataa acctcgccta tgagcggcac
      541 agcacgcatc tgtttattta taccaaaacc gaatacgagg cgctgttccg ccagtgcggt
      601 ttttccacgc tgacctgcgt acccggcgtg atggtgctga tggaaaacag cgccacgcga
      661 ctgaaacgct atgccgaatc gctgaaaaaa tttcgtcatc cagggaacaa gattggctgc
      721 attgtgatga acgccaatcc ctttacgaat ggtcaccgtt atctgattca acaagctgcg
      781 gcacagtgcg actggttgca tctgttttta gtcaaagaag attcttcacg cttcccctat
      841 gaagaccggc tggatctggt gttaaaaggc accgccgata ttccacgcct gactgtgcat
      901 cgcggctcag aatacatcat ctcccgcgct acgttccctt gctacttcat taaagaacag
      961 agcgtcatta accattgtta caccgaaatt gatctgaaga ttttccgtca gtacctcgct
     1021 cccgcgcttg gtgtaactca ccgctttgtc ggtactgaac ccttttgtcg cgttaccgcc
     1081 cagtacaacc aggatatgcg ctactggctg gaaacgccga ctatctccgc agcgcccatc
     1141 gaactggttg aaattgagcg gctgcgttac caggagatgc cgatatccgc ttcccgggta
     1201 cgtcaactgc tggcgaaaaa cgatctcacg gctatcgcgc cgctggtccc tgcagtcacg
     1261 ctgcattatt tgcagaacct gcttgagcac tcccgccagg acgcggcagc tcgtcaaaag
     1321 acccccgcat gagaaacagg tgaaaaatga aaataaacca gcccgccgtt gcaggcaccc
     1381 ttgagtctgg ggatgtgatg atacgcatcg ccccactcga tacgcaggat atcgacctgc
     1441 aaatcaatag cagcgttgag aaacagtttg gcgatgcaat tcgcaccacc attctggacg
     1501 ttctcgcccg ctacaacgtg cgcggcgtac agctgaatgt cgatgacaaa ggcgcactgg
     1561 actgcatttt acatgcacga ctggaagccc tgctggcacg cgccagcggt atcccggctc
     1621 tgccatggga ggattgccaa tgatttccgc ttcgctgcaa caacgtaaaa ctcgcacccg
     1681 ccgcagcatg ttgtttgtgc ctggtgccaa tgccgcgatg gtcagcaact ccttcatcta
     1741 cccggctgat gccctgatgt ttgacctcga agactccgtg gcattacgtg aaaaagacac
     1801 cgcccgccgc atggtttatc acgcgctgca acatccgctg tatcgcgata ttgaaaccat
     1861 tgtgcgtgtc aacgcgctgg attccgaatg gggtgttaac gacctggaag ccgtcgttcg
     1921 cggtggtgcg gacgttgtgc gtctgccgaa aaccgatacc gctcaggatg ttctggatat
     1981 tgaaaaagag atcctgcgta tagaaaaagc ctgtggtcgt gaacccggca gcaccggcct
     2041 gctggcggcg attgaatctc cgctgggcat tacccgcgca gtggaaatcg ctcacgcttc
     2101 cgagcgtttg atcggtatcg ccctcggtgc agaagactat gtgcgcaacc tgcgtacaga
     2161 acgctccccg gaaggaactg aactgctgtt cgcacgctgt tccattttgc aggccgcgcg
     2221 ctctgcgggt attcaggcgt tcgataccgt ctattccgac gctaacaacg aagccggatt
     2281 cctgcaagaa gccgcccaca tcaaacagct gggctttgac ggcaaatcgc tgatcaaccc
     2341 gcgtcagatt gatctgctgc acaacctcta cgcaccgacc cagaaagaag tggatcacgc
     2401 ccaccgcgtc gtagaagccg ctgaagccgc cgctcgcgaa ggcctcggcg tggtttccct
     2461 gaacggcaag atggtggacg gtccggttat cgatcgcgcc cgtctggtgc tctcccgtgc
     2521 agaactttcc ggcatccgcg aagaataagg caatcaaaat gacgcagaaa attgaacaat
     2581 ctcaacgaca agaacgggta gcggcctgga atcgtcgcac tgaatgcgat cttgccgctt
     2641 tccagaactc accaaagcaa acctaccagg ctgaaaaagc gcgcgatcgc aaactgtgcg
     2701 ccaacctgga agaagcgatt cgtcgctcag gtttacagga cggcatgacg gtttccttcc
     2761 atcacgcttt ccgtggcggt gacctgaccg tcaatatggt gatggacgtc atcgcgaaga
     2821 tgggctttaa aaacctgacc ctggcgtcca gctccctgag tgattgccat gcgccgctgg
     2881 tagaacacat tcgccagggc gtggttaccc gcatttatac ctctggccta cgcggtccac
     2941 tggcggaaga gatctcccgt ggtctgctgg cggaaccggt gcagatccac tctcacggcg
     3001 gtcgtgtgca tctggtacag agcggcgaac tgaatatcga cgtggctttc ctcggcgtcc
     3061 cgtcctgtga tgaattcggt aatgccaacg gctacaccgg taaagcctgc tgcggctccc
     3121 tcggctatgc aatggttgat gccgacaacg caaaacaggt cgtgatgctt accgaagaac
     3181 tgctgcctta tccgcataat ccggcaagca ttgagcaaga tcaggttgat ttgatcgtca
     3241 aagttaatcg cgttggcgat gctgcaaaaa tcggcgctgg tactacccgt atgaccacta
     3301 acccgcgcga actgcttatt gcccgtagcg ctgcggatgt gattgtcaac tctggctact
     3361 tcaaagaagg tttctccatg caaaccggca ccggcggcgc ttcgctggcg gtaacccgtt
     3421 tcctggaaga caaaatgcgt agccgcgata ttcgcgccga cttcgccctc ggcggtatta
     3481 ccgcgacgat ggttgatctg cacgaaaaag gtctgatccg caaactgctg gatgtgcaga
     3541 gctttgacag ccatgctgcg caatcgctgg cccgtaaccc caatcacatc gaaatcagcg
     3601 ccaaccagta cgctaactgg ggttcgaaag gcgcatcggt tgatcgtctc gacgtggtgg
     3661 tactgagcgc gctggaaatt gacaggcagt tcaacgttaa cgtgctgacc ggttctgacg
     3721 gcgtactgcg tggcgcttcc ggtggtcact gcgataccgc gattgcctct gcgctttcca
     3781 tcatcgtcgc gccactggta cgcggtcgta ttccgactct ggtggataac gtgctgacct
     3841 gcatcacccc gggctccagt gtcgatattc tggtcacaga ccacggtatc gcagttaacc
     3901 cggcacgtcc ggaactagca gaacgtctgc aggaagcggg cattaaagtg gtttccattg
     3961 agtggctgcg cgaacgtgcg cgtctgctga ccggtgaacc acagccgatt gaattcaccg
     4021 accgcgtcgt tgccgttgtg cgttaccgcg atggctcggt gatcgatgtt gtgcatcagg
     4081 tgaaggaata agccatgcac ctgcttcctg aactcgccag ccaccatgcg gtgtcaattc
     4141 ccgagctgct cgtcagccgg gatgaaaggc aagcacggca acacgcctgg ctcaagcacc
     4201 atcctgttcc actggtctcc tttaccgtgg ttgcgcctgg gccgattaaa gacagcgagg
     4261 tcacacgccg aatttttaat catcgcgtga cagccttgcg tgccttagcc acaaagcagg
     4321 gctggcaaat tcaggagcag gctgcactgg tttccgccag cgggccggag ggcatgttga
     4381 gcattgccgc cccggctcgc gacctcaagc tcgccaccat tgagcttgaa catagtcatc
     4441 ctctcgggcg gttatgggat atcgatgtcc tgacgcccga aggcgaaatt ctctcccgcc
     4501 gcgactattc actgcgcctc gccgctgcct gttgtgcgag caaagcgcag ccgtctgcgc
     4561 gcgtggaaaa acccatcaac tgaccgattt actcaaccgc atggaggcac tgctgaacga
     4621 tgtcgatgcc tgcaacgtca actaaaacca caaagcttgc gacgtcatta atcgatgagt
     4681 acgccctgct gggctggcgc gccatgctga ctgaagtcaa tctgtcaccg aaaccaggcc
     4741 tcgtggatcg cattaactgc ggtgcgcaca aagatatggc gctggaagat ttccaccgca
     4801 gcgcgctggc gattcagggc tggttacccc gtttcattga atttggtgcc tgtagtgcag
     4861 aaatggcacc ggaagcggta ctccacggat tacgcccaat tggtatggct tgcgaaggtg
     4921 atatgttccg cgccactgcg ggcgtaaaca cgcataaagg cagcattttt tctttagggc
     4981 tgctatgtgc ggcaattggc cgtttgcttc agctcaacca gccggttacg ccaacaaccg
     5041 tttgttctac ggcggcaagt ttctgccgtg gcctgaccga tcgcgaactg cgtaccaata
     5101 attcacgact gacggcaggt caacggttgt accagcagct tggccttacc ggcgcacgcg
     5161 gtgaagccga agcaggttat ccactggtga tcaaccacgc cttgccgcat tacctcactc
     5221 tgctggatca ggggttagat cctgaactgg cattgctcga taccttgctc ctactgatgg
     5281 ctaccaacgg cgataccaac gttgcatcgc gcggtggcga ggggggcctg cgctggctac
     5341 agcgcgaggc gcaaacatta ttgcaaaaag ggggcattcg aacccccacc gatctcgatt
     5401 atctccggca gttcgacagg gagtgtatcg aacgaaatct cagtccaggc ggcagtgccg
     5461 acctgctgat ccttacctgt tttttagcac agatttaatt atttaagcac ttgataaatt
     5521 tggaaatatt aattttcgga gaacccgtat gtctttagca aaagataata tatggaaact
     5581 attggcccca ctggtggtga tgggtgtcat gtttcttatc cctgtccccg acggtatgcc
     5641 gccgcaggca tggcattact tcgctgtgtt tgtggcaatg attgtcggca tgatcctcga
     5701 gccaattccg gcaacagcga tcagttttat tgcggttact atttgcgtta ttggcagtaa
     5761 ttacctgctc tttgatgcca aagaattagc tgacccagcg tttaatgcgc aaaaacaggc
     5821 gctgaaatgg ggcctggctg gtttttccag caccacggta tggctggtat ttggcgcatt
     5881 tatttttgca ttagggtatg aagtttccgg gttaggtcgt cgcattgccc ttttcctggt
     5941 gaaattcatg gacaaacgca cgctgacgtt gggttatgcg attgtcatta tcgacattct
     6001 gctggcaccg tttacaccgt ccaacaccgc gcgtaccggg ggtacggttt ttccggtcat
     6061 taaaaacctg ccgccgttgt ttaaatcatt cccgaacgat ccgtccgcgc gtcgtattgg
     6121 cggctatttg atgtggatga tggtcattag taccagtctg agttcgtcca tgtttgtcac
     6181 cggtgcggac caaacgtgct gggtctggag ttcgtcagca aaattgccgg tatccagatt
     6241 agctggttgc agtggttcct ctgcttcctg ccggttgggg ttatcttgct tatcattgcg
     6301 ccgtggcttt cctacgtgct gtacaaaccg gaaatcacac acagtgaaga agtggcaacc
     6361 tgggcgggtg atgaactaaa aaccatgggt gcgctgacac gcagagagtg gacgctgatt
     6421 ggccttgtat tgctcagctt aggtttgtgg gtatttggca gtgaagtcat taatgctact
     6481 gcggttggtc tgctggcagt ttcgctaatg ctggctctgc acgttgtgcc gtggaaagac
     6541 attacccgct ataacagcgc atggaacacg ctggtcaacc tggcaactct ggttgtgatg
     6601 gctaacggcc tgactcgttc tggttttatt gactggttcg ccggtaccat gagtacgcac
     6661 ttggaaggat tctcaccaaa cgcaacggtg attgtactgg ttctggtgtt ctactttgca
     6721 cactacctgt ttgccagcct gtctgcgcac accgcaacca tgctgccggt tattctggcc
     6781 gtcggtaaag gtattccggg cgtaccaatg gaacaactgt gtatcctgct ggtgctgtct
     6841 atcggtatca tgggctgtct gacgccgtat gcaaccggtc ctggggtgat tatttacggc
     6901 tgtggctatg tgaaatcaaa agattactgg cgtcttggcg caatcttcgg ggtgatttac
     6961 atctctatgt tgctgttggt tggctggccg attctcgcca tgtggaacta atacgttcgc
     7021 caaacgctga ataaatcatc acgcccgcca ggtaagccca cctggcgggc ttttttatga
     7081 tttaatagat agtattcagt gtatgtatga gttccacacc cattatgaaa gcattctgcc
     7141 gtaacgccgc gttgctcgcg gtttctctgc ttcccttatc ttctgccaac gccgtagcgt
     7201 tgcaggcaaa acagtatggc gattttgatc gctatgtcct ggccctctcc tggcaaaccg
     7261 gattttgcca gagtcaacac gatcgaaatc gtaacgaacg agatgaatgt cgcctgcaaa
     7321 ccgaaacgac caacaaaact gattttctga ccgtacatgg tctgtggcca ggattgccta
     7381 aatcggttgc tgcccgtggt gttgatgaac gccgctggat gcgcttcggt tgcgctactc
     7441 gcccaatccc gaatctacca gaagcacgcg ccagccgaat gtgttcatcg ccggaaaccg
     7501 ggttatcact ggaaacggcc gctaaactaa gtgaagtcat gccaggagct ggcggacgtt
     7561 cctgcctgga acgctacgaa tatgccaaac acggtgcctg ctttggtttt gatccggacg
     7621 catacttcgg tacgatggta cgcctgaatc aagaaattaa agagagcgaa gccggaaaat
     7681 tccttgcgga taattacggt aaaacagtga gccgccgtga ctttgacgcc gcctttgcca
     7741 aaagctgggg aaaagagaac gtgaaagcag ttaagctaac gtgccagggt aaccctgcgt
     7801 atttgactga aattcagatc tcgatcaaag ctgacgccat caacgctccg ctttctgcaa
     7861 actcattctt gccacaacct cacccaggta actgtggcaa aacctttgtg attgataaag
     7921 cgggttatta atcctctgat ttacaaaaca cgctaattcg tcttccataa ccatgccgat
     7981 taagaattat acataatgcg ctgcattgag gaattccctg aatgtgacgc aaatcacttc
     8041 aaagagtgag caacagtcag tatcatcatg tgagttaaac cctcgccgcc tgacggtgag
     8101 ggttttcttt tgggtatgtt tatctgcgac actcgcagta ccgacgacat ggagtaaaaa
     8161 tgtccagacc aactatcatc attaacgacc tggatgccga acgcatcgat attctgctgg
     8221 agcaacccgc ctatgctggt ttgccaatcg ccgacgcgtt aaacgcagag ttggatcgcg
     8281 cccaaatgtg ttcgccagaa gagatgccac acgacgtggt gacaatgaac agccgggtta
     8341 aattccgcaa tcttagcgat ggcgaagtgc gtgtgcgcac gctggtgtat ccggcaaaaa
     8401 tgaccgatag caatactcag ctttccgtta tggctccggt aggtgccgca ctgctggggc
     8461 tgcgcgttgg cgattccatt cactgggaac ttccgggcgg cgttgcaacc caccttgaag
     8521 tgctggaact cgaataccag ccagaagctg ctggcgacta cctgctttaa tcccatctgc
     8581 acaagcgata actcgctttg cccagaggat gcactcgcat cctctttacg caaaatcagc
     8641 aatttttcct ggttctcact gttcactttt gctgcaaaat tattccgttt cgtttgtggg
     8701 aataaccatg gcagaagcat tttacatcct gataggattt ctgattatgg cggcgattat
     8761 cgtcatggct gtcctttacc tcgaaaacca ctcctgacga tcatattgtt ccccccggca
     8821 tcgcattcac cagacctgaa accgccttct gcgccgcctc tgcgctttgt gcaccgggta
     8881 ccagaatcac cttacggcac tcctcttcac gtttttcgaa aatctcatat ccccgggcgg
     8941 cctcttcaaa cggcatatag tgggtaacaa tttcttctgg tttcagtaat cctttctcaa
     9001 ttaacggtaa taattctccc agccatgcgt gaacgtgggt ctgtcccatt ttaaacgtca
     9061 accctttatc aaaggcgtcg ccaaacagga aaccgtgaat aaatccagcg tagacgcccg
     9121 gtacgctaac aatgccgcaa cgctttaccg ctgctgccct ccagtttcag gttagtcagc
     9181 accgtttccg tggtgctgcc tttcgcttca aaaccgacgg cgtctattac tgcatccacg
     9241 ccccggtgac ctgccgtttg ttcaataatt gactgtgccg gatcgctatc ttcatcgaaa
     9301 ttaatcggga tcgcgccgta gcggtcagcg gcgaaacgca agcggtaggg atgatgatca
     9361 acaacaaaaa tctgttccgc accgagcaac cgtgcacagg cgattgtcaa caatcccaca
     9421 ggaccagcac catagactgc aacgcttgaa ccttgttgga tctgcacatt ttttgctgcc
     9481 tgccatgccg ttggcagaat atcagaaagg aaaagggctt tatcatctga aagcaaaggc
     9541 ggtactttaa acggccccac attccctttc gggacgcgga catattccgc ctgcccgcca
     9601 ggaacgccac catacaggtg actataacca aacaatgccg cgggagcagg tatctgtttt
     9661 ttattgagcg cagcgccttt acccgcattg gtattttcgc aggcggcata ttgctgtaat
     9721 cgacagaaaa aacagtcgcc acaagcaatg acgaacggaa ttaccacccg gtcgcctttt
     9781 tgcaaatttt ttacgtcctt cccggtttca acgacttccc ccataaattc atgaccaaaa
     9841 atatcgccat gtttaacctg gggtattttg cctcgataaa gatggaggtc agagccacag
     9901 atcgccgtta ccgtaatacg cagaataata tcatctgcct gttcaatgcc cggatcggga
     9961 acattttcta cctgaacgtg atgtgggccg tgataagtca atgctttcat gcaacctcca
    10021 gagacagttc aaaaaagagt cagtctttaa gggtagctat cacagcggga aaacgggcgg
    10081 taccgcaggt tttgggagaa ttcctagtgt tcagtgagaa tatattaatc acagaaaccg
    10141 ttcgtaagga aaaagaacca gacaaatatc agggataaat attataatca tggagaatga
    10201 tttgttcatg attaacaggg agaaagggtt atgtataagg caatcattat gccagttgat
    10261 gtatttgaaa tggaattgag cgacaaagct gttcgccacg ctgaattcct cgcccaggat
    10321 gacggagtta ttcatctact tcacgtacta cccgggtcag ccagcctgag cctgcaccgt
    10381 tttgccgctg atgtgcgtcg ttttgaagag catctgcaac atgaagcaga agaacgtctg
    10441 caaacgatgg tcagccactt caccatcgat ccttcccgca ttaaacaaca tgtccgtttt
    10501 ggtagcgtgc gggatgaagt caatgagttg gcagaagaac tgggggctga tgttgtagtt
    10561 atcggttctc gcaacccatc gatttcgacc catctgttag gttctaacgc ctcgagcgtg
    10621 atccgccacg ccaatctgcc tgtactggtc gttcgctaac agataaaagt atttatgccg
    10681 ccctgcctgg aacatcccgg cagggctttt taatgccata aaaaagccgc caggtttgac
    10741 cccggcggct aagcaattgc aggtgaatct tacttcttct tatgcagttt tggtgcgaat
    10801 caggtagtca aaagcactca gagaggcttt ggcaccttcg ccagtggcga tgatgatctg
    10861 cttgtacgga accgtcgtac agtcacccgc tgcgaacacg cctttgacgt tggtttcaca
    10921 tttcgcatca atgataatct cgcccatgcg gttacgttcg actgcgcctt ccagccagtt
    10981 ggtgttcggc agcagaccaa tctggacaaa aataccggcc agttcgatgt tgtgaatatt
    11041 gccgctgaca cgatctcgat attccagacc aacgactttg ctgccgtcgc ctttcacttc
    11101 cgtggtttgc gcattcagaa taatgtcgac gtttttcagg ctgcgcagtt tgtcctgcag
    11161 aacctggtcg gctttcattt ctggcgcaaa ttccagcagc gttacgtgct caacgatacc
    11221 cgccaggtcg attgccgctt ccacgccgga gttaccgccg ccgataaccg ctacgcgttt
    11281 gcctttaaac agcgggccgt cgcagtgcgg gcagtaggtc acgcctttag tgcgatactg
    11341 atcttcgccc ggaacgttca tgttgcgcca ttttgcaccc gtcgccacga taatgctgcg
    11401 tgctttcagt accgcgccag aagctgtttc aatctggtgt agaccacctt caaccgctgc
    11461 cgggatcagt ttgctggcgc tctggctgtc gatcacatca acgtcgtatt catcaacgtg
    11521 aactttcagt gcgcctgcca acttctgccc ttcagtcttc ggtacagaaa tgtagttttc
    11581 gatatcaacg gtatcgagga tctgaccacc aaaacgttcg cccatcagac cggtacggat
    11641 gcctttacgt gcggagtaaa ttgctgccgc tgcacccgcc gggccggaac caacgattaa
    11701 tacgtcataa gcatcacgct tgttcagctc ttctgccgca cgattttccg cgccagtatc
    11761 aattttggca acgatttcag tcaacgtcat gcggccctga ccaaactctt tcccgtttac
    11821 gaacactgcc ggaacgccca tcacgttgcg atcggtgatt tcgttctgga aggtgccgcc
    11881 gtcaattgca gtgtgcttga tgcgcgggtt caatacgctc atcaggttca gcgcctgcac
    11941 cacgtccggg cagttatggc aagagagcga gtaataggtt tcgaattcaa aatcaccgtc
    12001 aatatggcga atctgctcca gcagagactg cgcttctttc gacggatgac caccggtcca
    12061 cagcaacgcc agtaccagcg aggtgaactc gtggcccagc ggggagcctg caaaacgtgg
    12121 tccctggttg gaacctgggt tggtgatcag gaaagacggc ttacgcactg gcaagctgtt
    12181 atcttcttta aaggtgactt tgtctgacag ttctgcgatt tcagccaaca gttccttgat
    12241 ttctgccgat ttagcgctgt catccagcgt ggcaattaac tcaacaggct tggtcaattt
    12301 ctcaaggtaa gccttgagtt gagttttcat atttgtgtcg agcatgttta tctcctgggc
    12361 tttaaacatc atcatgcaag cggcctctgc ggctacctgg atgcagcttg catcatggtg
    12421 cggggaagaa aatcgggtgc cgcagcaccc gaagaattag gtgaatttcc gaagtatttc
    12481 gcgtcagagc aaggcgacta gcctgtgaat ccccgggagc ttacacaagt aagtgaccgg
    12541 ggtgagcggg cgacgccaac gccgctatgg cgtgaaagac gaaggaaatt tagattttac
    12601 caaccaggtc cagagacgga gccagagttg cttcaccttc tttccattta gccgggcaaa
    12661 cttcacctgg gtgagaagct acgtactgag ctgctttgat tttacgcagc agatcagacg
    12721 cgtcacggcc aatgccttca gcggtaactt cgattgcctg gatgataccc tgcgggtcaa
    12781 caacgaaggt cgcacggtca gccagacctt catcttcacg catgttgtcg aagttacggg
    12841 tcagggcgcc agtcgggtcg ccgatcatcg catatttgat tttagcgatg gtttcagagc
    12901 tgctgtgcca tgctttgtgg gtgaagtgag tatcggtaga tactgcgtat acgtctacgc
    12961 ccagtttctg cagttcttcg tagtggtcag caacgtcacc cagttcggtc gggcatacga
    13021 aagtaaagtc agccgggtag aagaagaaga cgctccagcg gccttcggta tctttttcgg
    13081 tgatttcgat gaattcgccg tttttgaatg cctggttttt aaaaggttta attttggtgt
    13141 taatcaagga catctatact tcctccgtgt tttcgatgag atgtaaggta accaattttt
    13201 gccgattcgg ctaatgcgtt tccattatca aatcgataag ttttacctta caaccttcgt
    13261 aagacaactt tgtgaacttt gcaccgccag cggcgataaa aagtaaaatg gccccctttc
    13321 caggtgaccg tgtaatctga actacccttt ggtatcttca gcgcctgcct gcaggctgag
    13381 gtgttgccct tacaaatgca acaacgacat ggattacaac accctcataa acaaagggca
    13441 atcacctgat ctaagctctt acctatgaca gtgataggtt atgcctttta ctcgactttt
    13501 gcactgactg aaaagaacaa attaatgtta aaaaagatac ttttactggc tctgcttcct
    13561 gcaatcgcct tcgcagagga acttccttcc ccagtaaaag cgattgaaaa acagggcatt
    13621 acaatcatca aaacgttcga tgcccccgga ggaatgaaag gttatctcgg aaagtatcag
    13681 gatatgggcg tcaccatcta cctgactccc gatggtaagc acgctatctc tggttacatg
    13741 tacaacgaga aaggtgaaaa cctgagtaac acacttatcg aaaaagaaat ttacgcacca
    13801 gccggacgcg aaatgtggca acggatggaa caatcccact ggctcctcga cggtaaaaaa
    13861 gatgcgccgg tcattgtcta cgtcttctcc gatccgttct gcccatattg taaacagttc
    13921 tggcagcagg cgcgcccgtg ggtagattct ggcaaagtgc aattaagaac attgttggtt
    13981 ggggttatca agccagaaag cccggcgaca gcagcggcaa ttcttgcctc caaagatccc
    14041 gcaaaaacct ggcaacaata tgaagcctct ggttgcaagc ttaagctaaa cgtgcctgca
    14101 aacgtaagta cagagcaaat gaaagtgtta agtgacaatg agaaactgat ggacgatctg
    14161 ggggcaaatg tcacgccggc tatctattac atgagtaagg aaaatacgct gcaacaggcc
    14221 gtggggttgc ccgatcagaa aacgcttaat atcattatgg gaaataaata aaaggtaatt
    14281 catggatggt tgaactatat ccctgctttt caaccatcct a
//
LOCUS       DAZSIO010000102        14318 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZSIO010000102 DAZSIO010000000
VERSION     DAZSIO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.102"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..3052
                     /locus_tag="ACHX5T_002742"
                     /pseudo
     CDS             1..3052
                     /locus_tag="ACHX5T_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3062..6708
                     /locus_tag="ACHX5T_002743"
                     /pseudo
     CDS             3062..6708
                     /locus_tag="ACHX5T_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6769..7176
                     /locus_tag="ACHX5T_002744"
     CDS             6769..7176
                     /locus_tag="ACHX5T_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8467878.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7718..8806
                     /locus_tag="ACHX5T_002745"
     CDS             7718..8806
                     /locus_tag="ACHX5T_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFR8467879.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8817..11096
                     /locus_tag="ACHX5T_002746"
     CDS             8817..11096
                     /locus_tag="ACHX5T_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFR8467880.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            11089..12225
                     /locus_tag="ACHX5T_002747"
     CDS             11089..12225
                     /locus_tag="ACHX5T_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR8467881.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            12222..14225
                     /locus_tag="ACHX5T_002748"
     CDS             12222..14225
                     /locus_tag="ACHX5T_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8467882.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3546 a         3755 c         3654 g         3363 t
ORIGIN      
        1 tgggattatg cgaataatgt cattattatt ctgccgtctg gcattgcacc tgactacttg
       61 gagcaatatt ccagatttga attacgccta cgtaaacatt tatcactggc ggaagagtct
      121 ctctggcaaa aatgtgcaca aaaacttatt gccgcaattc cacatattcc agaatggcgg
      181 caaccattaa ttgctttgtt attacccgaa aaaccagaaa ttgcacatga aattgcccag
      241 catctactgg ggcaaaaaaa attaccctcg cttgagtggt taaaaatagt ggcgactgat
      301 gagcacattc ttgcctcatt agaaaaatat cacgagccat atgccatttt tgatgattac
      361 tattgtggtg cgatatggtc agccaccgta ttacaggagc aaggtgttgc agccctgccc
      421 cgatttgctc cctatgccgc aagtgactac tgcgccgatg tgttgcgtca tatcaatcat
      481 ccgttcgcat taacactgct tatacgtgta gccgggcata ctaaacgctg tcacgatcga
      541 atgacgaaag cctgcgctgc gttcccacac gcagcactgg cagcactggc ggaacttcta
      601 gtgcaaaaag aagagaatag ttggcgcatt atgctaatga cgatgtttat ctcgcaacca
      661 gcactggcag aacaggtcat tccctggctt tcgacacccg cagttgcagt gctgaaatca
      721 tgccagcacc aactgacaca gccctcaaac catgccagcg ccgatctact gccagccata
      781 gtagtctccc caccatggat ttcgaaaaag aaaaaatcgc cgattccggt gctggattta
      841 gcgccattag gcatcgagcc aatctgttat ctgacagaag aaatcagtaa tcaacttttg
      901 gcgaaatata tctggtattc aaaacacatc acggttagcc atgaagaaag tactaccaac
      961 ctgttggcaa ggatgggttt tcaacgacgg atcgctggta catatattaa agctcccgaa
     1021 gcggtagttg aggcatggct aaatgaagat tattcaacct tactaagtga atttaaggtg
     1081 tttcattcac ctaccgggca ttattggcag ttggggattt tgacaacatt gccgctggag
     1141 gctggagaaa gcagtaaaag catggaatgc ccttactcta tctccacata ccgataccga
     1201 atacgccatg ttacattttg gactcaaagg tttacctggt ttagttaact caattacacg
     1261 ctatccacag gaagccttgc ccatcacgaa ttacttcgca gcgagtgagc tggctcctgc
     1321 cgtcgcccgt gccttcaaca aactcaaaac gctacgcgaa aacgcccgca cctggctgtt
     1381 gaaatacccg gaacatgccc ttaccggcct gctgcctgcg gcgctcggca aagccggtga
     1441 agcacaggat aacgcccgcg ctgccttgcg tatgcttacc gaaaacggtc atcagccatt
     1501 actgcaagaa atcgcccggc gttataacca gtcggaagta acagacgcgg tgaacgctct
     1561 gcttgcgctc gatcccttag ataatcaccc gacaaaaatc cccactcttc cgacctttta
     1621 tcagccatcg atctggacgc gcccggtatt aaaagcaaac gcccaatcac tgccagataa
     1681 cgccctcctc cacctcggcg aaatgctccg cttccctcag gaagaggctc tgtatccggg
     1741 attattgcag gtgaaagacg cctgtaccgc tgactctctg gctgaatttg cctgggatct
     1801 gtttaccgcc tggcagaccg ctggcgcgcc gtcgaaagag agttgggcgt tcactgcgtt
     1861 aggcgttctc ggtaacgatg acaccgcccg caaactgacg ccattaatac gcgcctggcc
     1921 tggtgaatcc cagcataaac gcgccaccgt tgggttggat attctcgccg ctatcggtag
     1981 tgatatcgcc ctgatgcagc ttaacggcat tgctcagaaa ctgaaattca aagcattaca
     2041 ggagcgggca aaagaaaaga ttgccgacat tgccgagagc cgcgaactca cggtagcgga
     2101 gcttgaagat cggttagcac cggatctcgg tctggatgat aacggttcgt tgctgctgga
     2161 ttttggccca cgccagttca ccgtcagctt tgatgaaacc ttaaaaccgt ttgtgcgtga
     2221 tgtttccggc agccgcctga aagacctgcc aaaaccaaac aaaagcgatt atgagtcaca
     2281 ggcgaacgat gctgttaacc gctacaaatt gctgaaaaaa gatgcgcgta ccgtcgcggc
     2341 ccagcaggta gcaaggcttg aatccgccat gtgcctgcgt cgccgctggt cgccggaaaa
     2401 cttccaactc ttcctggttg tgcatccgct ggttcgccac ttaacccgcc gtctgatttg
     2461 gggcgtttat agcgccgaca accagctact ggcctgtttc cgggtagcgg aagataacag
     2521 ctacagcacc gctgacgatg atcttttcac cctgccggaa ggcgatatct ctatcggcat
     2581 tcctcacgtt ctggaaatat caccgacgga tgctgtcgcc tttggtcagc tttttgccga
     2641 ctacgaactg ctaccaccgt tccgtcagct tgaccgtaac agttacgccc tgacagaagc
     2701 cgagcgcaac gccagcgaac tgacccgctg ggcaggcaga aaatgcccga gcggccgggt
     2761 catggggctg gcaaataaag gctggataaa gggcgaacca caggatggag gctggatgat
     2821 caaacctctc ggtcgctggt cgttaatcat ggaaatcgac gaaggttttg cggtgggtat
     2881 atcaccagcc gaacttagcg ctgagcagct cttaagcaag ctgtggctat gggaaggcaa
     2941 agcggaaagc tatggctggg ggagtaattc aacacaggaa gcgcagttct ccgtactcga
     3001 tgccatcacc gccagcgagc taattaacga tattgaagcc ctgtttgaat aaggaaagag
     3061 catggacaag gaattaccgt ggctggcgga taacgcccaa ctggaactga aatataaaaa
     3121 aggcaaaaca ccactcagtc atcgcaattg gccgggcgaa ccagtgcctg ttatcactga
     3181 aagcatcatt cagacactgg gtgatgaatt gctacaaaaa gctgagaaga aaaaaaacat
     3241 tgtctggcgt tatgaaaatt tttcaccgga gtggcagtcc gccattacgc aggccatcaa
     3301 cttgatcggc gaacacaaac cctcagtccc ggcccgcaca atggcggcac tagcctttat
     3361 cgcgcaaaat gacaaccaac agttgctcga cgaaatcgtc caacaagagg ggctggaata
     3421 tgcgactgag gtggtgattg cacgccagtt tattgcacgg tgttatgaga gtgatcctct
     3481 ggtagtcaca ttgcagtatc agaacgagga ctatggctat ggttatcgct cagaaaccta
     3541 taacgaattc gatctccgac tgcgtaaaca cctctctctg gcagaggaaa gctgctggca
     3601 gcgttgcgcc gacaaactca ttgccgcact gccaggaata accaaagttc gccgcccttt
     3661 tattgcgctg atcctccccg aaaaaccaga aatcgccaat gagttggtaa gccttgaatg
     3721 cccgcggact cattttcatt ctaaggagtg gttaaaagtt gttgctactg accccaaagc
     3781 ggtgagaaaa ctcgaacgct actggagcca ggatatattt agcgatcgag aagccagcta
     3841 catgtcgcat gaaaaccact tcggctacgc ggcctgcgct gcccttttgc gcgaacaagg
     3901 actggcagtc gttccgcgcc tcgcgatata tgcccataaa gaagattgcg gcagtctgct
     3961 ggtacaaatt aaccatccgc aagtcatccg caccttgctg ttagttgctg ataaaaacaa
     4021 acccagcctg caacgtgtgg ctaaatacag taaaaacttc ccccatgcga cactcgccgc
     4081 actggcagaa ctgctggcgt taaaagaacc accagcccgc ccaggttatc caatcatcga
     4141 agacaaaaag ctgcctgcac agcaaaaagc tcgagatgaa tactggcgta cgctgttaca
     4201 aacgctgatg gcatcgcagc cacaactggc agcagaagtg atgccgtggt taagtactca
     4261 agcccgggca gtgctgaata gttatttatc ggcaccgccc aaaccagtta ttgatagcac
     4321 cgataacagc caaatgcctg aaatcctcgt ttcaccacca tggcgtggga gaaagaaaaa
     4381 gacttccata cgtcactttt cgctgcctga tcttacgctt gcaccgcaaa cgtacaggtc
     4441 gccgaataaa cactggacgc cggaacagca aactgcaatt gaccatttta gcgccatgtc
     4501 ttttgacgaa cgtcttgcta cgagaggagc ggatatcttc ttgcgggaat taggttttga
     4561 cgttcatagc ctggaattta ataagcgtct tcttaatgga cagttggatg cctttgatgc
     4621 tcagcaactg caagaataca catcaatagg actattgcgt acttatcacg cgcatcaggc
     4681 caaatctaaa cagtaccggg aagacgccac caatgcatta ctggcacaag atagcattgc
     4741 gttgatggga gggtggcgaa cttttcagca cggattttac tatggtaatg aacagcgttg
     4801 ggggatttgg aatctgtacc ttatcgcgca gatgccgcgc gaaatggcgg tatcgtgctg
     4861 gcaacaaatt gtcgaggctg attttcatta caccggggtg gaatatttgt taagtgtgct
     4921 gggtaccgat gctttgcctg gattaaacac cgcctttgcc cgacatccaa aagagatatt
     4981 tccgctgctg attcatttcg gcgcaacaga actggccctg cccattgccc gcgtctggca
     5041 cctttttgcg ggccagcgta atctggctcg ccagtggatt ttacaatggc cggaacatac
     5101 ggctactgca cttatccctc ttgtcttcac caaatctagc gataaaagcg aagctgcatt
     5161 acttgccctg cgtttacttt acgaacatgg gcatggcgaa tggctacaaa ctgtagccaa
     5221 tcgttggcag cgtacagatc tatggcctgc cctggaacaa ttacttaaac agagtccaat
     5281 agagatttac ccggcacgca ttccaaaagc ccctgatttc tggcatccgc aaatgtggtc
     5341 caggccgcgc cttatcacta ataatcaacc tgttaccgat gacgctctgg aaattatcgg
     5401 cgaaatgctg cgctttaccc aggggggacg tttttatagc gggctggaac aactgaaaac
     5461 gttctgccag ccacaaacgc tggcagcttt tgcctgggat ctcttcactg cgtggcaaca
     5521 agctggtgcc cccgcaaaag acaactgggc atttctggcg ttaagtctct ttggtgacga
     5581 aagcacggca cgggatctaa cgacacatat cctcgcctgg ccacaagaag gcaaatctgc
     5641 ccgtgcggtc agtggcatga acatccttac cctgatgaat aatgatatgg cgctgatgca
     5701 gctgcatcat atatcgcaac gggcgaaatc ccgcccctta cgtgataacg cggcggaatt
     5761 tcttcaggta gtcgcagaaa atcgcgggct aagccaggaa gagttagcgg acagattagt
     5821 cccaacactg ggccttaatg atccgcaggc gttgagtttt gattttggtc cccggcagtt
     5881 taccgttcgc ttcgatgaaa acctcaaccc ggttatcttt gatcagcaaa acgttcgcca
     5941 gaaaagtgtt ccccggttgc gcgccgatga cgatcaactg aaagcgcccg aggcactggc
     6001 ccgactaaaa gggttaaaaa aagatgctac tcaggtgagc aaaaacctgc tcccgcgtct
     6061 tgaagctgcc ctacgtacca cccgacgctg gtcgctggca gattttcatt ctctgtttgt
     6121 taatcatccc tttacccgtc tggttaccca gcgattaata tggggggtta tccggcaaat
     6181 gaaccgcgtc gtttactcaa cgcctttcgt gtggccgcag agggggagtt ctgcaatgcg
     6241 caagatgagc caattgacct gcctgcggac gctctgattg gcattgccca cccgttagaa
     6301 atgacagcag aaatgcgcag tgaatttgca cagctttttg ccgattacga aattatgccg
     6361 ccttttcgcc agttgtcgcg ccgcacggtg ctgctcacac ctgacgagtc aaccagtaac
     6421 agcctgactc gctgggaagg taaatccgct accgttgggc aacttatggg aatgcgatac
     6481 aaaggctggg agtcaggtta tgaggacgca tttgtctatg acctgggtga gtaccggctg
     6541 gtccttaagt tttcacccgg ttttaaccac tacaatgttg atagcaaagc gctaatgagc
     6601 ttccgttctc ttcgagtgta ccgtgacaat aaatccgtca cttttgccga acttgatgtg
     6661 tttgatttga gtgaggcgtt aagcgcacct gacgtcattt tccattaaaa caaagcgggc
     6721 agtgatgcga ctgcccgtta tctacacgac ttaccagcgg ggaaagcgat gatcgtgcaa
     6781 aaagagctgg ttgctattta cgattatgag gtccctgtac ctgaagatcc gttttccttc
     6841 agacttgaga tccataaatg ctctgaatta tttacaggtt ccgtctaccg actggaacgg
     6901 ttccggctac gtccaacatt tcatcaacgt gatcgagagg atgttgaccc gctaataaat
     6961 gatgcgttga tttatataag agatgagttt attgatgagc aaaaattacg aggcgaatca
     7021 cctgaacctg taatagtaat ttttaattgt aaactacaga atatattcaa ccaacaaata
     7081 gaattatata ctctagataa ttcaagtcgc agcacatgca acttgaagta tgacgagtat
     7141 agctttatta aaggggggaa cggtattccc ccttagatgc gtttcaattt gcccgttact
     7201 tcaacaaatc aatcaaccgt aacagagcca ctaatagctc cagtacagca atgagcacgg
     7261 ttaacttttg tgtcagcgtg tccatccgct ctccttatta aggagcacaa caccagcacc
     7321 ttacccttac actgcctgtc gatactggtt tacgtttgtt gagtcgccat gttgcactcc
     7381 ggatcagagc actacctgcg gcaccacccg tccggtagcc aggacttgaa ataggttcga
     7441 tccggcgcaa cttcccaaca gtcaggatta taaatctcat tatcttaatt gcaacactgt
     7501 ccagaggtgg ttataattcg ttcgttggaa cacgatgttg cacaggcagt ggtgttaggc
     7561 ctgaaaatag taaataaaag aacctgcctc accagcagtt ttttttattt actgtgatct
     7621 gctttccaga tatttttcgc tcaaacaact aatgcgccaa acatttattg cgcgtaaaat
     7681 atcgtttatt tcattaatac atttcaggga tgaatatatg tcaccgcaga ataatcatct
     7741 gcagcgtccg cctgctgctg tgttatacgc cgatgaactg gcaaaattaa aacaaaatga
     7801 taacgcacct tgcccgcccg gttggcagtt aagtttgcct gcggcccgtg cttttatcct
     7861 tggcgatagc gcgcaaaata ttagccgtaa agtggtgatt agtccctccg ctgtcgaacg
     7921 tatgttagtc actcttgcca ccggacgcgg tttgatgttg gtgggggaac ctggcaccgc
     7981 aaaatctctc ctgtctgaat tactggcaac ctctattagc ggcgatgccg ggttaaccat
     8041 ccagggcggc gcatctacta ccgaagatca aatcaagtac ggctggaact acgccctgct
     8101 tatcaatcat ggtccatcaa cggaagcgct ggtccctgcc ccgctttacc agggaatgcg
     8161 cgacggcaaa atcgtccgtt ttgaagagat aacgcgtaca ccgctggaag tgcaagactg
     8221 tctgttaggt atgctttctg acagggtgat gacggtgccg gaactcactg gtgaagccag
     8281 tcagctctat gcgcgagaag gctttaatat tattgccact gccaataccc gcgatcgcgg
     8341 cgtcaatgaa atgagtgctg cgctaaagcg tcgcttcgat tttgaaactg tctttccgat
     8401 tatggatttc gcccaggagc tggagcttgt cgccagtgct tcggcgcgtt tgctggccca
     8461 tagcggtatt ccgcataaag tgcctgacgc ggtactggag ttactggtcc gcaccttccg
     8521 cgatctgcgt gccaacggcg aaaagaaaac gtcaatggat acactaacag cgattatgtc
     8581 caccgctgaa gccgtgaacg tggcccacgc cgtaggcgtc cgcgcctggt ttttagcgaa
     8641 tcgcgcaggt gagccagccg acctggtgga ctgcattgcc ggaaccatcg tcaaagataa
     8701 cgaagaagat cgcgctcgtc tgcgccgtta cttcgaacag cgtgtcgcca cacataaaga
     8761 agctcactgg caggcttatt atcaagcccg ccaccgcctg ccgtgaggaa agatgcatga
     8821 gcgagccgtt aattgtcggc atccggcatc atagtccggc ctgcgcccgg ctggtgaaat
     8881 cgttaatcga aagccagcgg ccacgatacg tgttgattga aggccctgct gattttaatg
     8941 accgggtaga cgaactgttt ttagcccacc agcttccggt agctatttac agttattgcc
     9001 agtatcagga cggtgcagcc cccgggcgtg gtgcctggac gccatttgct gaattttcgc
     9061 cggagtggca ggcgctacaa gccgatcgtc gtattcaggc acaaacttac ttcatcgatt
     9121 tgccttgctg ggcgcagagt gaagaagagg acgattcgcc tgatacgcaa gaggaaagcc
     9181 agaccttgct gctgcgtgcc acccgcatgg ataacagcga caccctgtgg gatcacttgt
     9241 tcgaagatga aagccagcaa actgcattac cctctgcact ggcgcactat tttgcccaac
     9301 tgcggggcga ctcccccggc gatgctctaa atcgtcagcg cgaagccttt atggcccgct
     9361 ggattggatg ggcgatgcag caaaataatg gcgacgtgtt agttgtctgc ggtggctggc
     9421 acgctccggc actggcaaaa atgtggcgcg aatgtccgca ggtaattaac acgccagaat
     9481 tgccgtcgct ggcagatgcc gttacaggtt gttatctcac cccctacagt gaaaagcgcc
     9541 ttgatgtgct ggcaggatac ctttcaggaa tgcctgcccc ggtctggcaa aactggtgct
     9601 ggcagtgggg cttacagcag gcgggagaac aactgctaaa aacggttctc acccgtttgc
     9661 gccagcacaa gttgcctgct tcgaccgcgg atatggctgc cgctcatctg catgcgatgg
     9721 cactggcaca gttgcgcggt catacactac cgttacgcac tgactggctg gatgccatag
     9781 caggatcgct gattaaagaa gccctgaatg cgccgttgcc gtggagctat cgcggcgtta
     9841 ttcatcccga taccgatccg attctgctaa cgttgataga cacattagcg ggtgacggat
     9901 tcggtaaact tgccccttct acgccacaac cgcctctgcc aaaagatgtc acttgcgaac
     9961 tggaacgtac cgcaatctct cttccggcgg agcttacctt aaatcgcttt aaccccaatg
    10021 gactagcgca aagtcaggtg ttacatcggc tggcaatact ggagatccca gggattgtac
    10081 gccagcaggg aagcacactg acacttgcag gcaacggtga agaacgctgg aaattaaccc
    10141 gcccgcttag ccagcatgcg gcattgattg aggccgcctg ctttggtgcc acactcctgg
    10201 aagccgcacg ccataaatta gaagccgata tgctggacgc gggtggaatc ggcagcatta
    10261 ccacatgtct tagccaagcg gcgtcagcgg gtctggcgtc cttcagtcaa caattactgg
    10321 agcaactcac attattaatc gcccaggaaa atcaatttgc cgaaatgggc caggcgctgg
    10381 aagtgctata tgccttatgg cggctggatg aaattagcgg tatgcaaggc gcgcagatat
    10441 tacaaacgac gttatgcgcg gctatcgatc gcacgctgtg gctgtgtgaa tccaacggca
    10501 gaccggatga aaaggagttt cacgctcact tgcatagctg gcaagcgttg tgtcatatcc
    10561 tgcgcgatct acatagcggt gtgcaactcc ccggcgtttc gctttctgcg gcggtagcct
    10621 tgctggagcg acacagtcag gcgattcatg tcctggcact ggatcgcggc gcgactcttg
    10681 gtgcgctgat gcgtctggaa catcccaacg ctagtgccga agcggcgctg acgatgctgg
    10741 cgcagttatc cccggcacaa tccggcgagg cgctgcacgg tttgctggca ttagcccgtc
    10801 atcaactggc ctgtcagccg gcatttatcg ccggtttcag cagtcattta aatcaactaa
    10861 gtgatgccga ttttaccaat gccctgcccg atttacgcgc ggcaatggcc tggctaccgc
    10921 cacgagaacg cgggacgctg gcgcatcagg tgcttgagca ttatcaactg gcgcaacttc
    10981 ccgtttcggc actgcaaatg ccgttgcatt gtccaccgca agccattgca catcatcaac
    11041 aactcgaaca gcaggcactg gcatcgctgc aacactgggg agttttccat gtctgaactg
    11101 aacgatcttc tgaccacccg tgagctacaa cgctggcgat taattcttgg cgaagcggca
    11161 gaaacgacgc tttgtgggct ggatgacaac gcccggcaga tagatcacgc gctggagtgg
    11221 ctgtatgggc gcgatcctga acggctccag cgtggtgaac gctctagtgg attaggtggc
    11281 tcaaatctca ccactcctga gtggatcaac agtattcaca cgctgtttcc gcaacaggtg
    11341 attgagcggc tggaaagcga tgccgtactg cgctacggca ttgaagatgt ggtgacaaat
    11401 ctcgacgtgc tggaacgtat gcagccttcc gaaagcctgc tacgcgccgt tttgcacacc
    11461 aaacatctga tgaaccccga agtactggct gccgcccgca ggattgtgcg ccaggttgtg
    11521 gaagaaatta tggcgcgact ggcaaaggaa gttcgtcagg ctttttctgg tgtccgcgat
    11581 cgtcgtcgcc gctcatttat tccactggcg cgaaactttg atttcaaaag tactctgcgc
    11641 gccaacctgc aacactggca cccgcaacac ggcaagttgt atatcgaatt cccccgcttt
    11701 aacagccgca ttaaacgcca aagcgagcaa tggcaactgg tcttactggt tgatcaaagc
    11761 ggatcgatgg tcgattcggt gatccactct gctgtgatgg cggcctgttt atggcagtta
    11821 cccggcattc gtacccatct ggtggcgttt gacacaagcg tcgttgatct cacggcagac
    11881 gttgccgatc cggtagagtt attaatgaaa gtgcaattag gcggcggaac caatatcgcc
    11941 agtgccatgg agtatggtcg gcaacttatt gaacaacctg caaaaagcgt cattatcctc
    12001 gtgagcgatt tttatgaagg gggttcatca tcattgctga cgcaccaagt gaaaaagtgt
    12061 gtccagagcg gcatcaaagt gctgggactg gcagcgctcg atagcacagc aacaccttgc
    12121 tatgaccgcg atacggccca ggcgctggtt aatgtcggcg cacaaatagc cgccatgaca
    12181 ccgggcgagc tggcatcatg gcttgcggag aatcttcagt catgaattca ctacgtccgg
    12241 aattattaga actgacaccg caggcactga cggcgttaag caatgccggt tttgttaagc
    12301 gcagccttaa ggaactggaa aatggcaacg tcccggaaat cagccatgag aacagcgctt
    12361 taatcgccac cttcagtgac ggtgtccgta cccagctggc gaacggccag gcactgaaag
    12421 aggctcagtg cacctatggg gccagcggca tgtgccgtca tcgcgtgatg ctggtgttaa
    12481 gttatcaacg actttgtgct accgcccagc ctacgggaaa agaagaggaa tgggatccgg
    12541 caatctggct ggaagaactg gctacccttc ccgatgccac ccgcaaacgc gcacaggcgc
    12601 tggtcgctaa aagcatcacc attgagttgt tctgtgcgcc gggtgaaatt ccctctgccc
    12661 gcttaccgat gagcgatgtg cgtttttatt cccgcagcag tattcgtttc gcccgttgtg
    12721 attgtattga aggcacactt tgcgaacatg tcgtactggc agtacaggcc tttgtccagg
    12781 caaaagcgca gcaagcggaa ttgactcatt taatctggca gatgcgcagc gaacacgtca
    12841 catcatctga cgatccgttt gccagcgaag aaggcaaaac gtgtcgtcaa tatgttcaac
    12901 aactaagcca ggcattatgg ctgagcggca tcagccagcc gcttattcac tacgaggccg
    12961 ctttcagccg cgcgcagcaa gcagcggaac gctgcagctg gcgatgggtg agtgaatcgc
    13021 tacggcaact gcgcgccagc gttgatgcct tccacgcccg cgccagccac tatcatgccg
    13081 gagaatgctt acgtcagctt gcggcattaa acagtcgatt aaattgcgca caagagatgg
    13141 cccggagcga cagtgttggt gaagttcctc ctgtgccgtg gcgcacggtc gttggctctg
    13201 gcattgccgg agaagcaaag cttgatcatc tgcggctggt gtctttaggt atgcgttgct
    13261 ggcaggatat tgagcattat ggtttacgca tctggtttac cgatcccgac accggcagta
    13321 ttttgcacct ttcgcgcagt tggccgcgaa gtgaacagga agactcaccg gcagctacgc
    13381 gtcggctgtt tagttttcag gctggcgcac tggcgggtgg gcaaattgtt tcacaagcag
    13441 caaaacgcag tgccgatggc gagctgctgt tagctacccg caaccgctta agcagcgttg
    13501 taccgctgtc gcctgatgcc tggcaaatgt tgagcgcgcc gttacgccag ccgggcattg
    13561 tggctttgcg ggaatattta cgccagcgtc cccccgcctg catacggcct cttaatcagg
    13621 tcgataactt atttattctg ccggtcgctg agtgtatttc gctcggttgg gatagcagcc
    13681 gccagacgct ggatgcgcag gtaatcagcg gcgaaggtga agataatctg ctgacgttat
    13741 cactgccagc ctcagccagc gcgccttatg ccattgaacg catggcggcg cttttgcaac
    13801 aaacagacga ccccgtgtgt ctggtttctg gctttgtcag ttttgttgat gggcaattga
    13861 cactggaacc acaggtaatg atgacaaaga ctcgtgcctg ggcgctggac gcagaaactg
    13921 cgcctgtggt ggtatcacta ccttctgcca gcgttctgcc tgtgccgtct actgctcatc
    13981 agttgctgat gcgctgccag gcgttactta ttcaactgct ccataacggc tggcgctatc
    14041 aggaacagag cgctattaat caggcagagt tgctggcgaa tgacctcacc gcggtgggtt
    14101 tttatcggct ggcacatgtg ttggcacaat ttcgtaatac agaaagcgag gcacgggtag
    14161 aagcaatgaa taacggtgtt ttgctttgcg aacaattatt ccccatgctt cagcaacaag
    14221 gatgaaatag tgctttttac taagagttct actccagttc cggactgctc acgccacggt
    14281 attaggcata tcctatatag cccctggtga gagtcacc
//
LOCUS       DAZSIO010000103        14251 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZSIO010000103 DAZSIO010000000
VERSION     DAZSIO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14251
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.103"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..104)
                     /locus_tag="ACHX5T_002749"
     CDS             complement(<1..104)
                     /locus_tag="ACHX5T_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HFR8467883.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(145..600)
                     /gene="csgB"
                     /locus_tag="ACHX5T_002750"
     CDS             complement(145..600)
                     /gene="csgB"
                     /locus_tag="ACHX5T_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFR8467884.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
     gene            1354..2004
                     /gene="csgD"
                     /locus_tag="ACHX5T_002751"
     CDS             1354..2004
                     /gene="csgD"
                     /locus_tag="ACHX5T_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFR8467885.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2009..2398
                     /gene="csgE"
                     /locus_tag="ACHX5T_002752"
     CDS             2009..2398
                     /gene="csgE"
                     /locus_tag="ACHX5T_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFR8467886.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2423..2839
                     /gene="csgF"
                     /locus_tag="ACHX5T_002753"
     CDS             2423..2839
                     /gene="csgF"
                     /locus_tag="ACHX5T_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFR8467887.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2866..3641
                     /locus_tag="ACHX5T_002754"
                     /pseudo
     CDS             2866..3641
                     /locus_tag="ACHX5T_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
     gene            complement(3705..4196)
                     /locus_tag="ACHX5T_002755"
     CDS             complement(3705..4196)
                     /locus_tag="ACHX5T_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR8467888.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4298..4852)
                     /gene="ycdY"
                     /locus_tag="ACHX5T_002756"
     CDS             complement(4298..4852)
                     /gene="ycdY"
                     /locus_tag="ACHX5T_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFR8467889.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4876..5613)
                     /gene="ycdX"
                     /locus_tag="ACHX5T_002757"
     CDS             complement(4876..5613)
                     /gene="ycdX"
                     /locus_tag="ACHX5T_002757"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFR8467890.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPCRLLNFLESRGMAPIAEFADL"
     gene            complement(5668..6606)
                     /gene="ghrA"
                     /locus_tag="ACHX5T_002758"
                     /pseudo
     CDS             complement(5668..6606)
                     /gene="ghrA"
                     /locus_tag="ACHX5T_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6841..6928
                     /locus_tag="ACHX5T_002759"
     tRNA            6841..6928
                     /locus_tag="ACHX5T_002759"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6875..6877,aa:Ser,seq:gga)
     gene            complement(7078..7359)
                     /locus_tag="ACHX5T_002760"
                     /pseudo
     CDS             complement(7078..7359)
                     /locus_tag="ACHX5T_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7641..8645)
                     /locus_tag="ACHX5T_002761"
     CDS             complement(7641..8645)
                     /locus_tag="ACHX5T_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HFR8467891.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8717..9277)
                     /locus_tag="ACHX5T_002762"
                     /pseudo
     CDS             complement(8717..9277)
                     /locus_tag="ACHX5T_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(9362..9559)
                     /locus_tag="ACHX5T_002763"
     CDS             complement(9362..9559)
                     /locus_tag="ACHX5T_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFR8467892.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(9571..10062)
                     /locus_tag="ACHX5T_002764"
     CDS             complement(9571..10062)
                     /locus_tag="ACHX5T_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFR8467893.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(10059..10433)
                     /locus_tag="ACHX5T_002765"
     CDS             complement(10059..10433)
                     /locus_tag="ACHX5T_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFR8467894.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10480..10854)
                     /locus_tag="ACHX5T_002766"
     CDS             complement(10480..10854)
                     /locus_tag="ACHX5T_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFR8467895.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(11017..11238)
                     /locus_tag="ACHX5T_002767"
     CDS             complement(11017..11238)
                     /locus_tag="ACHX5T_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFR8467896.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(11301..11777)
                     /locus_tag="ACHX5T_002768"
     CDS             complement(11301..11777)
                     /locus_tag="ACHX5T_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFR8467897.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(11793..12266)
                     /locus_tag="ACHX5T_002769"
     CDS             complement(11793..12266)
                     /locus_tag="ACHX5T_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR8467898.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(12608..13429)
                     /locus_tag="ACHX5T_002770"
     CDS             complement(12608..13429)
                     /locus_tag="ACHX5T_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFR8467899.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(13529..13702)
                     /locus_tag="ACHX5T_002771"
     CDS             complement(13529..13702)
                     /locus_tag="ACHX5T_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFR8467900.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(13822..>14251)
                     /locus_tag="ACHX5T_002772"
     CDS             complement(13822..>14251)
                     /locus_tag="ACHX5T_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8467901.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3643 a         3492 c         3410 g         3706 t
ORIGIN      
        1 ccaccaccgt ggttaccgcc gccgccgtac tgaggaacaa cacctgccag agcgctaccg
       61 gagaatacga ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg
      121 attgatttaa aagtcgaatg gtaattaacg ttgtgtcacg cgaattgcca tttgcgactg
      181 tctctgcact acaactgccg ttttttgagt accatactgt gtaatatttg ctttattacc
      241 agaacctttc tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc
      301 gttggcactg cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt
      361 tgcccgattg ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg
      421 taactgagca ctattattag tcccagcctg accaattatg gctgcctgat taaatgaaga
      481 cttactcaat tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc
      541 ggctgcaatc ccaggcgcac ccagtattgt taacatcata aataacaatt tgtttttcat
      601 gttgtcaccc tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta
      661 acgcaacctg tattttgtta acgctgcgtt acgatggaaa gtatgtctgc ggaaatattt
      721 ttaataactc acccgcgcgt tgtatttttc tttttttgtt cataccaaag tattaaaacc
      781 cgcgtaaata cgctgttatc tacgcaaaaa atattttgtt ttctcttaaa tctccgtttt
      841 ccgctatcaa aaatcaccag acagtcattc ttcttgcccg tcgctgattg ctgcgatatg
      901 tcttgcgcac aagccgtgac aaacctcgtc tttttgcagt aaaaaaattg tccacggagg
      961 tgtggagaaa aaacaaaaac gttttacatg acgaaaggac tacaccgaaa tattttttat
     1021 atgtattatt agtaagttag caccatttgt atgatttttt aaaattgtgc aataaaaacc
     1081 aaatgtacaa cttttctatc atttctaaac ttaataaaac cttaatgtta acattttaat
     1141 ataatgagtt acattttgtt acatgtttaa cacttgattt aagatttgta atggctagat
     1201 tgaaatcaga tgtaatccat tagttttata ttttacccat ttagggctga tttattacta
     1261 cacacagcag tgcaacatct gtcagtactt ctggtgcttc tattttagag gcagctgtca
     1321 ggtgtgcgat caataaaaaa gtggggtttc atcatgttta atgaagtcca tagtattcat
     1381 ggtcatacat tattgttgat cactaaacct tctttgcagg cgacagctct cttgcagcac
     1441 cttaaacaat cgctagcaat tacaggaaaa ttacataata ttcaacgttc tctggacgat
     1501 atctcttcag gctctattat tcttctggat atgatggaag cggataaaaa gcttatccat
     1561 tattggcagg atactttgag caggaaaaac aacaatatca aaatattgtt gctaaatacg
     1621 cctgaagatt acccgtaccg cgacattgaa aactggcctc atatcaacgg cgttttttat
     1681 gccatggagg atcaagaacg tgttgtcaat gggttgcaag gcgtcctgcg tggcgaatgc
     1741 tactttacgc aaaagcttgc cagttacctg attacacatt ctggtaacta tcgttataac
     1801 agcacggaat cagccctcct tactcatcgg gaaaaagaga tcctgaataa gctgcgtatc
     1861 ggcgcgtcta ataacgagat cgctcgttcg ttgttcatca gcgaaaatac ggttaaaacg
     1921 catctttata atcttttcaa gaagatagcc gtaaaaaacc ggacacaagc ggtttcctgg
     1981 gcaaacgata acctcaggcg ataaagccat gaaacgttat ttacgctgga ttgtggcggc
     2041 agaatttctg ttcgccgcag ggaatcttca cgccgttgag gtagaagtcc cgggattgtt
     2101 aactgaccat actgtttcat ctattggcca tgatttttac cgagccttta gtgataaatg
     2161 ggaaagtgac tatacgggta acttaacgat taatgaaagg cccagtgcac gatggggaag
     2221 ctggatcaca ataacggtca atcaggatgt tattttccag acttttttat tcccgttgaa
     2281 aagagactcc gagaaaactg tcgtctttgc actgattcaa actgaagaag cactaaatcg
     2341 tcgccagata aatcaggcgt tattaagtac ggacgatttg gcgcatgatg aattctaaat
     2401 aaaaaattgt tcggaggctg caatgcgtgt caaacatgca gtagttctac tcatgcttat
     2461 ttcgccatta agttgggctg gaaccatgac tttccagttc cgtaatccaa actttggtgg
     2521 taacccaaat aatggcgctt ttttattaaa tagcgctcag gcccaaaact cttataaaga
     2581 tccgagctat aacgatgact ttggtattga aacaccctca gcgttagata actttactca
     2641 ggccatccag tcacaaattt taggtgggct actgtcgaat attaataccg gtaaaccggg
     2701 ccgcatggtg accaacgatt atattgttga tattgctaac cgcgatggtc aattgcagtt
     2761 gaacgtgaca gatcgtaaaa ccggacaaac ctcgaccatc caggtttcgg gtttacaaaa
     2821 taactcaacc gatttttaag ccccagcttc ataaggaaaa taatcatgca gcgcttattt
     2881 cttttggttg ccgtcatgtt actgagcgga tgcttaaccg ccccgcctaa agaagccgcc
     2941 agaccgacat taatgcctcg tgctcagagc tacaaagatt tgacccatct gccagcgccg
     3001 acgggtaaga tctttgtttc ggtatacaac attcaggacg aaaccgggca atttaaaccc
     3061 tacccggcaa gtaacttctc cactgctgtt ccgcaaagcg ccagggctta caaaacctgc
     3121 ttaacgagcg caaggttatt cgtgcggcac aagaaaacgg cacggttgcc attaataacc
     3181 gaatcccgct gcaatcttta acggcggcaa atattatggt tgaaggttcg attatcggtt
     3241 atgaaagcaa cgttaaatcg ggcggtgttg gggcaagata ttttgggatc ggtgcagaca
     3301 cgcaatacca gctcgatcag attgccgtga acctgcgtgt cgtcaatgtg agtaccggtg
     3361 agatcctctc ctcggtgaac accagtaaga cgatactttc ctatgaagtt caggccgggg
     3421 ttttccgctt tattgactac cagcgcttgc tggaagggga agtgggttac acctcaaacg
     3481 aacccgttat gctgtgtctg atgtcggcta tcgaaacagg ggtcattttc ctgattaatg
     3541 atggtatcga ccgtggtctg tgggatttgt aaaataaagc agaacggcag aatgacattc
     3601 tggtgaaata ccgccatatg tcggttccac cggaatcctg acagataaaa atgccggaat
     3661 taataatccg gcatttactg tttaagcgaa agcgtgagtg cccctcacgc tttatttttg
     3721 atttcctgct cacggtgagc cgtctttgca ctacgcgccg ccagccacag gccgctgttt
     3781 ttcattgcgt aaccaaatac cagcccaagc gccagcgaag gtaacaccag tttccagtca
     3841 ccctgcccgg caaacgtcgc gcatgcgcct ataaacgtcc ccggaacaaa tgaaagcagc
     3901 agctgtttgg cctgaataca catcagaaac gcgacaatac cggttatgac ataaccgaga
     3961 atttccagat gtggtgccag tgcactaccg taaataatga ccatcgccca caccacgcca
     4021 ctaagcaggg ttgcagcgga gatcgccagc cctttcagcc caccttgcgg gcaagcaaag
     4081 taggccgtac agcccaggaa gcccgcccag cttagtaagc ccagggaaac agccacccat
     4141 ccccagatac cggagagaat gcctgttgtg attgcaatag agagaagtat gttcatgccg
     4201 cgcatgatag cagaaaacgc ggccatgaat gagatcgagc acacatttta tgcaacaaca
     4261 aattatctgt tgcagttatg agtgacgatg atcacattta ctcttcagaa tcttcttcca
     4321 gctcgtccca cattgcacta atggcatcac gggttagcgg tgccatggtg cgccagaaag
     4381 gcgtggttgc atgggcctcc actttgccga ggaacgcacc acaccagggt aacagatact
     4441 cactgaacag tgtttccagt gcttcgctct catcttccgt tgactgatct tccagccagg
     4501 aagccgcaag cagcaatgtg ccgatgtgat cggctggcgt atccgccagt agcatccctc
     4561 gttcggaaag aaaagcgcgc acttccgctt ccgttgcgtc ctcaacccat gcgctacgat
     4621 atggcggcac ggcacattca tcgccgataa acaacgcatt gtaatcggca gagacttgcg
     4681 tcatatcaca gcttttctgt aaacgcgtca gtaactcatc ctgctccagt ggccagttcg
     4741 cagccagttt cccctcacga atcagagtaa acagcggcac cagtaaagga tcttgcggtt
     4801 ggcggtaata gagcgaaccc agcacacgac agaggataga aaactcgttc attaatatat
     4861 ttccattact agtaattaaa ggtctgcaaa ttccgcaatc ggtgccatac cgcgagattc
     4921 aaggaagttc agtaagcggc acggagaaac attcaaaatg cgctctagcg gaaaatctac
     4981 cgcgtcgagg attttaagac actcttcaaa ttcccccatg gtaaacgcgg tgtgagaatc
     5041 cgagcctaat gccacccaac ctccagcatc acgtaccgct gcagctaccg cacgacagtt
     5101 gtcttcactg cccttacgtg agtgtaaaaa tgaggaatta ttgatttcca gcgccacctg
     5161 atgtttcgcg gctgcttcag caacggcttt aacatctatt tcatatttcg gatttccggg
     5221 atggcttatt atatgcacat tgccgctggc gatagtggag atcatcgctt gtgtattggt
     5281 cgctttgtca tgtggcgcaa aaaccggctc atgaaaaccg gcaataatca gatccagcga
     5341 gtcaaacatt ttaccgctgc agtcaatttc accatcaaca tttttaatgt tagcttcaat
     5401 gccgcgcagg atccctaccc catcaaccac tcgcggccag atacgcatgt taatgaagtg
     5461 ccagtgatgc ggcgcatctt ccatatccgg gccatgatcg gtgatcgcaa aaagtttaat
     5521 gcccttttgt ttggcctggg caatgtaatc actcaatgtg ctatatgcat gtgtgctggc
     5581 aacggtatgc atatgaaggt cgacgggata cataactctc tcctgtattc aattcccgcc
     5641 aaggatagca ggaatcctga tgctttatta gtagccacgt gcgcggtcga cttgcccgca
     5701 gaccctctcc cctttttcga gctgggcaat agtgcgagaa atgtactcca cagcttcagc
     5761 gggacgggta atcgcggcga catgtggtgt tatcgtcacg cgtggatgtt gccagagcgg
     5821 actttcaggc ggtaagggtt cacgattaaa aacatccagc attgcgcctt taactttccc
     5881 gctatccagc gccgcgagca ggtcatcttc cacaacatga acaccacgcg ccaggttgag
     5941 gagatacgcg ccatcaggta atttttcgag taattgttga ttaataatgc cgacggtttc
     6001 aggggtattc ggtaacaaat taatcaatac ccgacattgg ctcagaaatg cagacagttc
     6061 ttcccgtccg gcaaagcttt gcacgccagg ccacgattta cgggttcgac tccagcaacg
     6121 cagcggaaag cgctaggttt gcagactctg agcaacttta cagcccagta cgcctgcgcc
     6181 caaaatgccg atggtaaaat cttcccgatg atattcaggc agcggttgcc aatgcgaact
     6241 attttgctgg atgcgataat cgtcaaaacg tcgaaaccaa tgcagcacct gactgacagc
     6301 atattcctgc atttgctcgc ccataccggt atcttccagg cgaaaaagtg gaacagaagg
     6361 gttcagcatt tcagggtgtg cctgtagctt gctcaaaata gaatcaacac cggccccgag
     6421 tgcgaacacc gctttaagat cgcgccctgc cagcatttca acaggaggat gccagactaa
     6481 aacataatca gcagagtcat tatctccgct tttccatgcc ctgactcttg cctgaggaat
     6541 agctttgcgc agtgcctcaa tccaccattg ggtatcgaac gttgggtgat aaaagatgat
     6601 atccatactg actcccgaaa agcgttgtgc gaaatttatt cgcacttatc gttatgatct
     6661 acaaaggcca ccagcataac aaatccgtgg tcggtggcaa aaaaagcaga tttcgcttat
     6721 taaaaccaca cattgattga atgtttgaat aaacgcgcga ttttttcaaa aagtttgttg
     6781 acctcaggtc atgatttccc taaattagcg cccgttccag caagacagga acgacaattt
     6841 ggtgaggtgt ccgagtggct gaaggagcac gcctggaaag tgtgtatacg gcaacgtatc
     6901 gggggttcga atccccccct caccgccaga ttataagtgc atgttattgt ttaacatgcg
     6961 cttttttatt tttccttcca gacagtatgc catcgggtat gccatctgaa accggtttat
     7021 atgccccgcg atcatatctt atcggacaat ttccgatctt tgagcatact ggcaaagcta
     7081 tactgccagc atatcaggat agtgttttgc gataatatca ttcagtctgt caaccagttc
     7141 tggttttctg aacgtgatat gcgcggagcc cttcttaaag tatctgatgc tgaacatctc
     7201 atcttcatag ctctctttac cctgaacaga ctgaatgtga tcatccagac gaagggtgat
     7261 gtttccccgg ttgtcgggga ttggtttacc gctgaacagg tgcaacattc tttccagatc
     7321 ggcgagtcgg tcagcctgtt gtccggtgat aagatgaaat ctatggtcac ccccgttttt
     7381 gcaacactga tttttgatta atggttggct tgcttaaatc tatccggcgt ctctgtgggc
     7441 aaggatgccc gcgccccgat gagttccgca cctgataatc ctaaaaatcc cctcggcttc
     7501 agtgagccat ttcatctgtt aggtttttaa tcaggtcgat ttgccgttca tgtcatcaat
     7561 aatcagtctt cgcaaaacca gaaaggtaac ttgtatctta ttgtttaaaa taaactaatt
     7621 gagttatatc agcatctata tcaggcctga tattcttttt tctgagtagt gattgcccag
     7681 gcaattcgag ccagtttatt tgccagagcg caggtaacca cattcgaatg cttcttgttg
     7741 agttgctccc gaacccactc agcaagtttt ccatgcttgc gttcaagtcg cattatgaat
     7801 gagcgggcac actgaaccag taaccggcgt aaatttttat cacctcgctt actgataccc
     7861 aacagagtcg attttcctcc agtgctatac tgccggggaa caagtcctgt ggaagctgca
     7921 aaatccctgc tgcatgagaa ctgtttcccg tcaccaagtt gcgacgatag taagctggca
     7981 gtaatcggac caacaccagg aattgtcata atgcgctgag cggtatcatc agcgttgatt
     8041 gactgactta attctgattc cagctctgct atttgctcaa caaggtaaaa gtagtgggta
     8101 tgcaacctca ccaaaaggcg gctcagatat tcggggattt cgtgttcagc caacacgaga
     8161 gacaaccgtt ttattacagc atcacctatc ggcaggctga ttccaaattc cagcaagaaa
     8221 ccgtggatct ggttggttgt tttcacctta tctctgatga gcgattctct gacccggtgt
     8281 aacgccctca tggcctgctg ggtctcagtt cttggctgga caaaacgcat tgatggtcgt
     8341 gaagccgctt cgcatatggc ttctgcatca acaaaatcat tcttattact tttgacaaac
     8401 gggcgaacga actgtggaga aatcagctta gtttcatgcc caaaatcaga gatacagcga
     8461 gccataaagt gtgcacccgc acaggcttcc attacaacaa ctgagggcgg acatgtcgcc
     8521 agaaactgca tcagtttagt acgggtaaat tttttacgca gaagagcctt accagacttg
     8581 tcttgacaat gaatgtggaa ggagtgcttt ccgagatcga taccaataag tgtgatgttc
     8641 tgcatgatgg tctctccgtt aaagggattt accttaacag cgtactgctg ttaaggtggg
     8701 ggtgaccata tcattatccc caccggtccc atctcaccag attgttgacg ataattttac
     8761 tgccaaattt acaggggcaa ttggttttgt aattccagtt cagccctctg aagacgttga
     8821 tcacccctcg ctcaaacacc tcatccttat tctggtgcag ttgttcaaat gtgctcagaa
     8881 tgttcgcttc gctgatttcc ggaaaattat cgtactccag tgaccggtac catgtatcac
     8941 gggcctgcgt atccattaat gacagcatcc cggaacgttg catcaggtca cgccagattt
     9001 cgcgatcaag attccgggta atggctttca ttgctgtttc gggtttctcc atcagccagc
     9061 agccgcagcg aaaatcctgt ttcatggccc agtccggggc agttttacct ccgatgcttt
     9121 ccgtcagcac cgagatttca gccagttgtc ggataagtgt gtcgatttgc aacagtgtac
     9181 tctttcgtac agccagaatg tggcgaatgc tggttgagca gataacgtcc gtatgcccgt
     9241 tgaccacttc cggacgggag attgtgatat ctgacataat cgatattcct ttttgcagat
     9301 aaatgaaaac gccagccctg tgcaggctgg cgtcagagga atgaagtctg gtgagtgagc
     9361 ttcaccggtt ctggtgcaga agctgcagat gacgcagggt acggacatcc tcccgggcct
     9421 gttcgataca gggcaacagt gctgctgaat ccgttttatc ctcatcgttg tcaaagataa
     9481 ttcccgatcc gcagtcgata ttgtcctgca gccacgcaat cagaatatcc agcgctgttt
     9541 ccgtggttaa tgatttcatg ttgttcctgg ttaccagtcg aaagtgggta aacccggcag
     9601 gcattcagcg ctggcatcca gatgaatgag actgacgcca taacgccgga tgagtgtgac
     9661 gaccagacga cggaacgttt ttgacagccc caggcgttta agccgcagaa ccgggtatga
     9721 ccacgcatcg gtacgtaaca gatatccgct acctgtaaaa tgaatccatt ctgattcacc
     9781 gaagtcactg gtctggtgtg acagcgagta caaccaggcg ttgtccgttt ccgtgatatg
     9841 tgcggtactg cagcgtatgc cggtaagggt agcaaatggt ggcgtgcatt ctgctgaggt
     9901 ctggtcagat tcatcaacaa cacgaaggga gtagccgtta tcgcagacca tgttaatcag
     9961 ttcagcgagc tcaataccat cgacgtcaac gacaatgcgc cctatgttca gtgcctgtac
    10021 gttaacgctg tcggcttcca gtgtcagggc cagtttcatc atttcgcctc cggatactta
    10081 cccagggtaa tgttatttac cgttctgtaa ttgtcgcggg tcatcaggcc ggtcgcccgg
    10141 cgagcccgga ggatatcaat actgtttatt aactgagagc ggggacaggt gctgaatccc
    10201 ggctggtcgg tacgcaccag cgcgtatttt tccacgagaa aattaactgc atcacacagt
    10261 gaaatgcctg cctcaatatg ctgctcaatc acacgttcat cgacaaacgg tgtgtcattc
    10321 agtgtgaggc cataatgctg gtccagcagt cggatgagca gtgtctgcca gatggtgacg
    10381 ggagacgggc agcgagatac ctcccgtaca tgtgtgtcgg ataatgtttt catcttaagt
    10441 tctctgtaac tggtagtgct aaaggtgaga gcttcggggt tatgaggtgg ttgcgggtgc
    10501 tggtgtcgga taaacagcca gataaacgta accacaggag ccgagggtgt cggcttcgca
    10561 ggtcagccct tttgcataca gcgtgacggt atgctgatat cggggattca gttcaccgct
    10621 ggtgagcatg agttccagtt gtttcatcag cagcggaaag gcctggtcca gatggtatgc
    10681 atctgcgtcg ctgaagcggc ctctgatacc ggcgcggtcg gcaaggtaat acaggcggtt
    10741 accctcttgc accagacgag cgccaaaaca gggcctcact gtgcagggaa gcccccacca
    10801 ggggcggtcg tgattgtcat cgggatgagt tatcccggag agtttgtctg acacgataaa
    10861 atcctctctg aaaagtggtt aagaatggtt taacgatgac aggtcagccg gcgcgctgac
    10921 cggtttcggt gaagatgtat gcattgatga gaatggcctc atccacagcg gcgtctgagg
    10981 tcatctggtc gtactcgttt cccgggtgac ggaacatcag ttcagggtga cactcatcag
    11041 ccggacatac gggccaaaac tgtccttacg gcgttctgca aacacggcaa gaacgccggg
    11101 aatatcctgc acttcacgac cagtatacgc ttcagcactg ccgtgccagc gatacttacc
    11161 ggtacagaac ggaaaaagac gggatgcagg atgctgttgg tgaatacgca tggcttcacc
    11221 acgggtgatg attctcatga tgtgatacct ctgaagacag aagataaaag tgaaaacagg
    11281 tgtgatgtgg ttgtgacggg ttaaagcaga ccatgttcgg caaaggagaa aacctggttg
    11341 ccaccgacta tcagatggtc cggcacccgg atatccacca ggcccagtgc ctgtaccaga
    11401 cgttcggtga taagccggtc tgccttactg ggtgtgactt caccggacgg gtgattgtgc
    11461 gccagcacca cggcagcggc attgtggtac agggcgcgtt taatcacttc ccggggatga
    11521 acttccgtac ggttgatggt gccggtgaag agggtttcac cggcaatcag ctgattctgg
    11581 ttgttcagat acagcacccg gaactcttcc cgctccagtc ccgccatgtt cagaatcagc
    11641 cattcccgtg ccgcacgggt ggaggtgaag gccacgccgg gttcatgaag atggcggtcc
    11701 agggtttgca gggcccgcag aatgagactg cgctcgccgg gcgtcatgtt tccgggcaga
    11761 aaggaaagct gctgcattgt ccctgcctcc attcagtcga tgatgcgcat aatggcgctg
    11821 cattccggat gctgcagggc gtaatcccgc agccggtaat aatggaccgt catggcataa
    11881 cactccgtac gacaggcatg atgactgtac gtcatcagac aggcagcaat gccggcggct
    11941 tccgggctca tttcagcgcg gttaccgttc atggcattga acagtaccca tgtttcgtca
    12001 tcatcgtcat ccggttcggg tgccataaat gccccgccgt tgttcagggt gtacagattc
    12061 cagataccac cgcagtagtc ttcacacaga cggtccatcc agccgaagac acggggttcc
    12121 agggtgaccc actgtggaat gaggccaaaa tgctgcggcc agaagctgat gcgctgttca
    12181 tcagggacgg gagtggcaac cagttgttgc aggccagtct ccggtgcaca ggccgaagcg
    12241 gtggtgttct gagacaatgt tttcatgtat gaaatccttg taatgtgaat aaatgagagg
    12301 gagtaaaggt cgggagctgg caacatcacg ttcatcactg accgtgatga cccggtaata
    12361 tcagggatga atgatattcg gctgaaattc cggacagccg tacgcgtaac cgttccggtc
    12421 acagacggat ggccagatgc cattgcctgt atactcagtc tgcacagact gatttctccg
    12481 ggacggtaaa gcagacgggt acaccaggcg cacaggtcat catcagaaat ggaacgacac
    12541 cccggcagcg cctccggggc gtcgggtgat tctggtgcct gaatcacgcc ttcagggaca
    12601 tgctgcctca gcgcaggctc tccagcagcg tttctgccat tacccacaat gcgcggttga
    12661 gcttaatatc ggtgtcgatg ttgtgaatgg cacgggtgtg gatacgtttt ccttttgcac
    12721 tgcggccgga aatcccgcct ttcagcatat tctcctggat ggtctgatat gcgctccaca
    12781 ggtctttacc gtaatcctcc ctgcgtcgtg gtgtcagaat gtcagcggtg gtgactggct
    12841 gatgttcgtc accataacgg taagtcagtg ccgcctgtgc cagcgcctgg cgtgccggtg
    12901 gcggcaggac cagcgactgc atggcatcac gcttctcctc tatccggtca aacacgccca
    12961 ccacctcgta agccccttca ataactttct ccaccacatt tccccggtgt ggaacacgca
    13021 attctcccag agactgaccg cagaccagtc cattggtaca aatggcacga aaatatccgg
    13081 gtaacatctg gtagctggat gaaccgtcat gagagttgag cagaataatt tcagggacat
    13141 gctgaccggt tatctgtccg gcccgccgca gacgcagcat gtgttttgtg tattcccggc
    13201 gacccgggtc acgcacacgt gtctggcagg cgaagaatgg ctgaaagcct tcccgctgca
    13261 ggctttccag tacggtgatg gtgggagtgt acgcatagcg tttactgcgg gaagtatgcc
    13321 ggtcttcacc gaaaatactg ggaacatagt gcatcagttc ttcgtgtgtc agcggacggt
    13381 cacggcgtat ctggtttgca gcataaccaa aacgactggc tagtcgcata atttgctcct
    13441 tatcggtggt taagatttac tggtgtaata aatgaaaaag ccacgtctcc cggagaagac
    13501 gcggcctgat ggacgaaatt aatgaggttt attgtctgag aagaccgtca ctggcgaacc
    13561 ttccggcgtc agtctctgcg ttgcggataa cccgacggaa atgagttccc tggtcatcgt
    13621 cgataaacac attgcggtaa gctgttgtga tgacaacagc ctgctcgcgt gagaaaggga
    13681 cttcgggcaa taaacgtgac atacaaccag acctgtattt ggttccctga aaatataagt
    13741 ccctgtcatc atggcagagc cgtatttact tttgatatca ataaattaat aacgcgaaga
    13801 tgaaccgcat aacttacgcc tttaatttgt tttgtttacg tagtccctga ccaggtacgc
    13861 gaattcgtga acgccagttt caccaaacca aatcaggtaa ccagggtcga tgttgtaata
    13921 gtaaccaacg actttatgcg tttttgggtt atagagtgtt acgatatagt cccagccgca
    13981 ggcgtgggct ttacaaccgc caccttctac ccaggtcgtt ccgttaatgt ttacaggata
    14041 cgcaccacca tcaggaccat ccatatagcc gtcttcccca aacattccgt ttaacccaac
    14101 agggtttaat gctttgtcga tagcagctct gtaggtagcg tcaccttcgt ataactcgta
    14161 cgttgttgga catgcaccag gctggtcagc ccgggctttt gagtcgcatt taagtgtctg
    14221 atttacagtg gcggcgtttg tgtcaggact t
//
LOCUS       DAZSIO010000104        14177 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZSIO010000104 DAZSIO010000000
VERSION     DAZSIO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.104"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..79
                     /locus_tag="ACHX5T_002773"
     CDS             <1..79
                     /locus_tag="ACHX5T_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8467902.1"
                     /translation="RAFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACHX5T_002774"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACHX5T_002774"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR8467903.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="ACHX5T_002775"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="ACHX5T_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR8467904.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACHX5T_002776"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACHX5T_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR8467905.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACHX5T_002777"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACHX5T_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR8467906.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACHX5T_002778"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACHX5T_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR8467907.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="ACHX5T_002779"
     CDS             5377..5835
                     /locus_tag="ACHX5T_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR8467908.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="ACHX5T_002780"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="ACHX5T_002780"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR8467909.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="ACHX5T_002781"
     tRNA            6887..6971
                     /locus_tag="ACHX5T_002781"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="ACHX5T_002782"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="ACHX5T_002782"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACHX5T_002783"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACHX5T_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR8467910.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACHX5T_002784"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACHX5T_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR8467911.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACHX5T_002785"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACHX5T_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR8467912.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="ACHX5T_002786"
     CDS             complement(10252..13995)
                     /locus_tag="ACHX5T_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR8467913.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         3502 a         3749 c         3402 g         3524 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgct
//
LOCUS       DAZSIO010000105        14110 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZSIO010000105 DAZSIO010000000
VERSION     DAZSIO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.105"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..415
                     /locus_tag="ACHX5T_002787"
                     /pseudo
     CDS             1..415
                     /locus_tag="ACHX5T_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555244.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            580..4698
                     /gene="pic"
                     /locus_tag="ACHX5T_002788"
     CDS             580..4698
                     /gene="pic"
                     /locus_tag="ACHX5T_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HFR8467914.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4849..5091)
                     /locus_tag="ACHX5T_002789"
                     /pseudo
     CDS             complement(4849..5091)
                     /locus_tag="ACHX5T_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(5130..6080)
                     /locus_tag="ACHX5T_002790"
                     /pseudo
     CDS             complement(5130..6080)
                     /locus_tag="ACHX5T_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6131..6706)
                     /locus_tag="ACHX5T_002791"
                     /pseudo
     CDS             complement(6131..6706)
                     /locus_tag="ACHX5T_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6710..7110
                     /locus_tag="ACHX5T_002792"
                     /pseudo
     CDS             6710..7110
                     /locus_tag="ACHX5T_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7513..8094)
                     /locus_tag="ACHX5T_002793"
     CDS             complement(7513..8094)
                     /locus_tag="ACHX5T_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8467915.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(8141..11998)
                     /gene="sigA"
                     /locus_tag="ACHX5T_002794"
     CDS             complement(8141..11998)
                     /gene="sigA"
                     /locus_tag="ACHX5T_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR8467916.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(12359..13363)
                     /locus_tag="ACHX5T_002795"
                     /pseudo
     CDS             complement(12359..13363)
                     /locus_tag="ACHX5T_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            13348..13725
                     /locus_tag="ACHX5T_002796"
                     /pseudo
     CDS             13348..13725
                     /locus_tag="ACHX5T_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13764..>14110)
                     /locus_tag="ACHX5T_002797"
     CDS             complement(13764..>14110)
                     /locus_tag="ACHX5T_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8467917.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         3541 a         3317 c         3459 g         3793 t
ORIGIN      
        1 catcgtgtgg tgacgagtct catcgtctat ggaaacgaca ttcgtgctgg atgcactgga
       61 gcaggcgttg tgggcccgtc gtccgtctgg caccatccat cacagcgata aaggctctca
      121 gtatgtgtca ctggcctata aggcgcgact aaaagaagca aaactgctgg catcaacagg
      181 gagtactggt gactcgtatg acaacgcgat ggcagagatc atcaagggcc tttacaaagc
      241 ggaggtaata caccgtaaga gctggaaaaa ccggacagaa gtggagctgg ccacactcac
      301 gtgggtggac tggtataaca atcgacgatt gctggaaagg ctgggccata tcccaccggc
      361 agaagcagaa aaagcttatt atgcttccat cggaaatgat gatctggcag cctgagttca
      421 cagataaaac actctccagg aaacccgggg cggttcagtt cacaaaaaca cattaatgca
      481 gtaactatat tttcctttct ggtgataacg tcgggttatc attagcttct tcagctattt
      541 tacttttata tcccttgtaa acatcatgga gaatccatag tgaataaagt ttattctctt
      601 aaatattgcc ccgtcaccgg ggggcttatt gctgtctctg aacttgcccg cagggtaata
      661 aaaaagacat gccgaagatt aacgcatatt cttctggctg gcattccagc aatctgtctg
      721 tgttactctc agatatccca ggcgggtatt gtccgttccg atattgccta tcagatttat
      781 cgtgatttcg ccgaaaacaa agggcttttt gtacctggtg ccaatgatat tccggtatat
      841 gataaggacg gaaaacttgt gggaagactg ggtaaagccc caatggccga tttcagcagt
      901 gtgagctcaa atggcgttgc tacgcttgta tcgcctcagt atatcgtcag cgtaaagcat
      961 aacggaggat atcggagtgt gagctttggt aatgggaaaa atacatattc ccttgttgac
     1021 cgtaataacc acccttctat tgacttccat gctccacgtc tgaataaact ggttacagaa
     1081 gttattccct cagcggtaac atcagaagga accaaagcca atgcttataa atacactgaa
     1141 cgttacaccg ctttttatcg ggtgggtagt ggtacgcagt acactaagga caaggacgga
     1201 aatttagtta aggttgccgg tggatatgct tttaaaacag gaggaaccac aggagttcct
     1261 ctgatatctg atgcaacaat agtctctaat cccgggcaaa cttataatcc tgtaaacggc
     1321 cctttacctg actatggagc ccctggggat agtggttctc ctttgtttgc ttatgataaa
     1381 caacaaaaaa aatgggttat tgttgctgta ttaagagcat atgcaggtat taatggtgct
     1441 acgaactggt ggaatgtcat accaacagat tatctgaacc aggttatgca ggacgatttc
     1501 gatgcccccg tagactttgt ttccggactg ggccccctga actggacata cgacaaaaca
     1561 tcaggcacag gtaccctgag ccagggcagt aaaaactgga ccatgcacgg gcagaaagac
     1621 aatgacctca atgccggtaa aaatctggta ttcagcgggc agaatggtgc aattatcctg
     1681 aaagacagtg tgactcaggg tgccggttat ctcgaattta aagacagtta caccgtatct
     1741 gctgaatccg gaaaaacatg gacgggtgcc ggcattatta ctgacaaggg gacgaatgta
     1801 acctggaagg tcaacggcgt tgccggtgac aacttgcata agctggggga aggaaccctg
     1861 accataaacg gaacaggtgt aaacccggga ggactgaaaa cgggagacgg tatcgttgta
     1921 cttaaccagc aggcagacac tgcaggtaat atccaggcct tcagttcagt gaacctcgcc
     1981 agcggacgtc cgaccgtggt gctcggggat gcccgtcagg tcaatccgga taacatttca
     2041 tggggatacc ggggaggtaa gcttgacctt aatggtaatg ccgttacctt cacccgactg
     2101 caggctgctg attacggggc ggtgattaca aataatgcac agcaaaaatc ccagctttta
     2161 ctggatctta aggctcagga tacaaatgtc agtgaaccga cgattggaaa tatatccccc
     2221 tttggtggta ccggcactcc aggaaacctg tacagcatga tactcaacag ccagacccgc
     2281 ttctatattc tgaaatctgc cagctatggt aacactctgt gggggaacag cctgaatgat
     2341 ccggctcagt gggagtttgt tggcatgaac aaaaacaaag cagttcagac agtaaaagat
     2401 aggatcctgg ccgggcgggc aaaacaaccc gttatctttc atggtcagct gaccgggaat
     2461 atggatgtcg ccattccaca ggtgccgggg ggaagaaagg taatctttga tggtagcgtg
     2521 aacctgccgg aaggtaccct gagtcaggac agtggcaccc tgatattcca gggacatccg
     2581 gttatccatg cctccatcag tggcagtgca ccggtcagcc tgaaccagaa agactgggaa
     2641 aaccgtcagt ttacaatgaa aacactgtcg ctgaaagacg ctgacttcca tctttcacgt
     2701 aacgcctcgc tgaacagtga cattaagtcg gataacagcc atatcacact gggaagtgac
     2761 agggcatttg tggataaaaa tgacggaaca ggaaattatg tcattccgga ggaaggtacc
     2821 tctgtcccgg acaccgtgaa tgacaggagc cagtatgaag ggaatattac gctgaaccat
     2881 aactcagccc tggatatcgg cagcaggttc accgggggga ttgacgctta tgacagtgcc
     2941 gtcagcatca cctctccgga cgtcctgttg acagccccgg gtgcttttgc cggcagttca
     3001 ctgacagtgc atgatggcgg tcatcttaca gcactgaacg gtcttttcag cgacgggcat
     3061 attcaggccg gtaagaacgg caaaatcacc ctgagcggta caccggttaa agatacggct
     3121 aatcagtatg cccctgctgt atatctgacg gacggatatg acctgaccgg cgataacgca
     3181 gcactggaaa ttacccgtgg agcacatgct tccggtgata ttcatgcctc tgcggcatca
     3241 acagttacca tcgggtctga cacgccggca gaactggctt ctgcggaaac ggctgcatcg
     3301 gcgtttgccg gcagtcttct tgagggctat aacgcagcat tcaatggtgc cataaccggt
     3361 ggcagggctg atgtcagtat gcataatgca ctgtggactc tgggtgggga ctctgccatc
     3421 cacagtctta ccgtcagaaa cagccgtatt agttctgaag gagaccgtac attccgtacc
     3481 ctgacggtga ataaactgga tgcaacaggc agtgattttg ttttgcgtac ggacctgaaa
     3541 aatgccgata aaattaatgt gactgaaaaa gccactggtt cagataacag cctgaacgtc
     3601 agctttatga ataatcctgc tcagggacag gccctgaata ttcctctggt cacggcaccg
     3661 gcgggaactt cagcagagat gtttaaggcc ggcacccggg tgacaggttt cagtcgggtg
     3721 accccaaccc tgcatgttga taccagtggt ggcaatacga agtggatact ggatggtttt
     3781 aaagcggagg ctgataaagc cgctgccgcg aaggctgaca gtttcatgaa tgccgggtat
     3841 aaaaacttca tgacggaagt taacaatctg aacaaacgta tgggtgacct gcgtgacaca
     3901 aacggtgatg ccggtgcctg ggcgcgcatc atgagtggtg ccggttctgc agacggtggt
     3961 tacagtgata attacaccca tgttcaggtc ggctttgaca aaaaacatga actggacggt
     4021 gtggacctgt ttaccggtgt cacgatgacc tataccgaca gcagtgcaga cagccatgca
     4081 ttcagcggaa agacgaaatc ggtggggggc ggtctgtatg cttcagcatt gtttgagtcc
     4141 ggtgcctata tcgatttgat tggtaaatat attcaccatg acaatgatta cacaggtaac
     4201 tttgctagcc tgggaacgaa acactacaac acccattcct ggtatgccgg tgctgaaacg
     4261 ggttaccgct atcacctgac agaggacacg ttcattgagc cgcaggctga actggtttac
     4321 ggcgccgtgt ccgggaaaac attccgctgg aaagacggtg atatggacct gagcatgaag
     4381 aacagggact tcagtccgct ggttggaaga acaggggttg aactgggcaa gaccttcagt
     4441 ggtaaggact ggagtgtgac ggcccgtgcc ggaaccagct ggcagtttga cctgctgaat
     4501 aatggagaga ccgtactgcg tgatgcgtcc ggggagaaac ggataaaagg agagaaggac
     4561 agccggatgc tgtttaatgt tggtatgaat gcgcagataa aggacaatat gcgctttggt
     4621 ctggagtttg agaagtcagc ctttggtaaa tataacgtgg ataatgcggt aaacgcgaat
     4681 ttccggtata tgttctgatg tgggggtaaa gggctgccgg aaggcagcct ttctcagaga
     4741 ctcttaaaat ataaaactga aggtcttcgg taagggggag tgtattagac gtcaactagt
     4801 tttgccgact ggcgcgagcc tgtttaagat tctgtgtaaa tgcctttctc agaagtgacc
     4861 gtccaggcgg tcaccgaact cgataataaa gcggctcatt gccattcgcc agtccttcaa
     4921 cggcatcgtc catttctggg acgcagactg gattgccagc cacaccactt ttttcaccga
     4981 gtcgtctgtc gggaacactt tacgcttttt gatcgcatgg cggatcacgc tgtttagcga
     5041 ctcgatggca ttcgtcgtat agatcacttt gcggatgtcc gttggataag cggtaagcgt
     5101 acagcgaggg ccgtattgac ggggatgtgt tattcagctg gcagtgctat gcgccacgga
     5161 agcagttcgc tgacccggtt gaccggccag tctgctatga cgccaagcac atggcgaagg
     5221 tagctttctg gatccacgtc attcagtttg cacgtcccga tcaggctgta cagtagcgcg
     5281 ccccgctcac caccatggtc agagccgaag aacaggaagt ttttacgacc cagactgacc
     5341 gcccgcaggg cattttcagc gatgttgttg tcgatttcca cccagccatt actgcagtac
     5401 acgttcaggg ccttccactg tttcagtagg tatgcgaacg cttttgccgt atccgagtga
     5461 cgcgacagtg ttttcatctg ttgctgtatc cggtcataca gtgactgcat cagtggcgcg
     5521 gctcttgctt ttcttgccgc cagacgctgt tctgctgaac atccccggac ttctgcctcg
     5581 atggtataca gttcaccgat acgctgcagg gcttccgtgg tgatgtcggt tggcactctt
     5641 gcatgcacat cgtggatttt tctccgggca tgggccatac acgcagcttc cgttattctg
     5701 ccggattcgt ataacgcccg gtaaccaccg taagcatctg cctgaagcac accgctgtaa
     5761 ccggctaggt gattctgtgg atggatacct ttccggtccg ggctgtacgc gaaccagacc
     5821 gccgggggca tctgtgaacc ggcgttacgg tcatcacgga catagaccca cagccgggca
     5881 gttcttgttt taccactgcc cggctcctgg accggaacgg ggatatcatc cgcatgaact
     5941 ttaccgggca tcagtacata ctggcgcagg acgtcataca gcggctccag cagttcagca
     6001 acggcaccgg tccagcgacc cagtgtggca cggctcagct ccacgccctg acgacggtat
     6061 atttctgact ggcggtataa ctgtcaacga cggatgaaaa gtggctcttc cgcaacctgg
     6121 gtggagattt tcatgccagt ggactcatta ccctctgcct gagtagttca aatcctgctc
     6181 gaccatacat ctggcgtttc agcatcttct tcaggcgatt tacatgacct tcgacaacac
     6241 cattactcca gcgactactg attgcctcac atatagctgc agcatcagct tccatccccg
     6301 ccgccacgcg ccgaaattct gctgaaccgc tttcgtggac tcgagtgaac caactgctta
     6361 gctgtgattt attttgggtt ttcagaatac ggtagaactc gagtgccagt tgctgcgcta
     6421 ttttcagctc aggttctttt tcacacatca gactaataaa tcgggaagca taattttctt
     6481 catccctggt tattctccag ggcatcaacc agcggcttac ccgggagact gaaggaagtc
     6541 tgacggcagt agtaaccggt gggttccagc ctttacgcca tttggcaacc gcatccctga
     6601 cgatggtttc actgcctgta aatccccggg ccaccatttc ccgccatatc cggctggcgt
     6661 tataattacc actttctcgt tgctctttta accactccct ccatggtgtc agcgaaaaac
     6721 gaaaccgctg ttgaaatccc tggaaagctg gttgcggaaa agatgaagac ccagtcgcga
     6781 cactcagagt tggcgaaggc gttcgcgtac gcacttaacc agtggccggc actgacgtac
     6841 tatgcgaacg atggctgggt ggaaatcgac aacaacatcg ctgaaaatgc cttgcgggcg
     6901 gtcagtctgg gtcgtaaaaa cttcctgttc ttcggctctg accatggtgg tgagcgggga
     6961 gcgctactgt acagcctgat cgggacgtgc aaactgaatg acgtggatcc agaaagctac
     7021 cttcgccatg tgcttggcgt catagcagac tggccggtca accgggtcag cgaactgctt
     7081 ccgtggcgca tagcactgcc agctgaataa cacatccccg tcaatacggc cctcgctgta
     7141 cgcttactca tcacctgcta ttaatccccg agtcagtact tcgcggcctg acgcgatcac
     7201 cctactcagt acttcagagc ccgcctccgg tacctgcctg gcgatcgccc ctgtgcaaca
     7261 aaataaatca accgttatca aaccacgatc gttcgtatgc gccgccgatg acgcgcgcct
     7321 gaaaaacgcg tcaatatgag ttccccggaa gcttacgata atacgcttat gaacattaca
     7381 acacgctcat gcggaacctg gatgggggaa ggaacggtga agctgttagc gaatgaccgg
     7441 cgtttataca aacgccggtc atgatgaatg atatttcaga atatacctcg ggctaaaata
     7501 ccgggacaga gatcaggcgt gattaatctg cagcattttt aataacagaa gtactgagct
     7561 tactttacct ttgatgatgt aatagttatt tccgtcggta ctcatttttc cctgaaataa
     7621 tgtcacggat gtttcttcct gctgatcctg caggcgcagt cgtaaggtca tatttttaat
     7681 gagctgagat ccgggtggca aggggtattt gctgtcagat actgggcgac tgagtctgat
     7741 gtggcgcgta tacagcccgt tttcgtacag ggtggcctct gttatggtgt cagtgaccag
     7801 catgtgggag acaatgctgt attctttttt aaagcaccca ccagaaacgg tgattgtaat
     7861 gataatggtc ttctcgatcg agatgtaaat acccgtttcg ttatactgtc cgtatcggaa
     7921 ccatgagaac tcagttgcat tccagccatg ctccagaata tgtgcatttt tttgcctgat
     7981 agtttctttt tttattcgct catgtttaag gcataacagg cagataaata atgtcagaaa
     8041 aagaaagcta ataatgagag caagagcgat atagtctgtc acagggtaat acatattctc
     8101 tccggtagaa gaagggccgc aaacgcggcc cgggctgtta tcagaaagag taacggaagt
     8161 tggcgttgat cgcgttatcc acgttgtatt taccaaatgc cgatttctca aactcaagac
     8221 cgaagcgaag attatcgcga atttcggcgt tgagaccaac attcatgaga atacgaccgt
     8281 ctttttcacc tttgatacgt ttctcaccgg acgcatcacg cagtacggtt tcaccgttgg
     8341 caaacaggtc aaactggtag ccaaggccgg cgcgggctgt gactttccag tccttaccgg
     8401 agaaggattt acccacatca acaccggtac gcccaatcag cggattaaag tccttatcct
     8461 tcatggtcac gctcattccg cgatcctgcc agtcgaaccg tttacctgat acggccccat
     8521 aaaccagttc tgcctgcggc tcaatccagg agtcttctgt tacatgatag cggtagcctg
     8581 cttcagcacc cacatacaag gaatgagaac tgtagtcttt tgttccgagt ccagcaaagg
     8641 tcgccgagta ctcattatca tggtgaacat acttactaat caggtcgata taggcaccag
     8701 agtcaaatat agcggaagca tacagacctg ccccgacgga cttcgtcttg ccactgaatg
     8761 cattactgct ggcatgactg tcggtatacg tcatagtcag accagtgaaa aggtcaagtc
     8821 catccagctc atgtttttta tccacaccaa tctgcacatg ggtgtagttg tcactgtatc
     8881 caccagaagc tgaacctgct ccgctcatga tgcgggccca tgcaccggcc tcgccattgg
     8941 tgtcacgcag atcccccatt cgtttattca ggttgttgac ttctgtcagg aaggctttgt
     9001 aattaccgga catatacgat tttgctgcac cggaagatgc tgtattttca gccaccttat
     9061 atccggtcag ggtccacgtg gtatgttcct gatcctcttt cgtgctgatc acaggagata
     9121 tgttgctgaa acctatggcc tgagtactgg ctttaaatat ctctttattg cttccttttg
     9181 gggctgttat caggtcaacg tttaatcctg agttattatt tcccactttc tgtatatcga
     9241 ccgacagact gttattgctt ccggttaact gatttttaac aatgagttta tccgattgat
     9301 ttacagactg gctcagatca gcccgcataa tcacagcagt attagtggca tccagtgtgt
     9361 ttactgtcac agtatggaaa cccgaattct ctatgttact aaacaacaaa agactgtcgc
     9421 gggtttttaa tgattgcagg gctgactgtc cgtttactct ccataaggtg ttatacatgc
     9481 tggctgaggc tgcaggagca tcaatacctc cggtataaga agtatcaaag ccacccagca
     9541 aacctgtagc cagcggtgtg tgtgtggttt tgtccaggtt atcttcttta tactggtcaa
     9601 caccaaacag aatagatgct tttttgtctg attttatatt accattgacc caggcataat
     9661 tttgagcctt taacgtggca ccatcttctg tcatattata tccatctgac atatagattg
     9721 ccgggctgta tttattattt tctgtagggg tgcctgttaa tgtcagggtt ccggccagtt
     9781 caatagcttt atctacatac atttttccct gggcggtaac agtagctcct ttgttgataa
     9841 tcaggtctgt attaattaag gataatgcac cagtcatagt aacagttgat ttatcaccaa
     9901 cagttatttt gctgttttga gcactcagac tgccgttgaa actactttcg cctgctgaat
     9961 ccccggagcg aagctgttgt ctgaatccaa aaccttcgcc agtaatgttt cttcctgaga
    10021 acatgtcaat gtatgcagtt ttatttccca gaacaatttg agagttttta gcctctatgt
    10081 gtccttttac attagcatcc cggcctatac tcagggttgc gttttctaaa tttaatttcc
    10141 ggaaattaaa tttacgactt tcccagtcgg gctgggaaaa agaggctatc tgattgttgg
    10201 acttatattc cgtattgtaa cgtttattta cagtgctttc ctggtccttt atggcagcag
    10261 aatagtcttt ttgacataaa aaaggaattg gacagttgtt attgccttct ttaaatatgg
    10321 catgttctgt cgcatgtcct tgtaatgtta actgtgcatt ccggacagag atatcgtttt
    10381 tgatatcgac tgagccatca aagatcagat tggcattatt gttatgttgc tgggtagtat
    10441 tgataatatg atttatgctt atattacctg atacattccc atggtacata tatgcatcag
    10501 tattagtaag tgataatgtt gattgcttca cgtttgagtt cgttacggtt gttcccgcat
    10561 ctgttgctgc gattttctga aatgactggt catagccgtt tagatccagt gttccgccat
    10621 ttctggtaaa atagatttcg ccattaccgc tttcgcttaa agcgtctttg gcatttattt
    10681 ttaccgtacc tttacctccg gccatgtaaa ttttattgaa ggctttttca gcactaagga
    10741 tgacagtccc attacctatt ttaaggttat ttccctgtgc tatttttaca tccagagtac
    10801 cactacctat tttatgcaga ttatctccgg caacgccctt aacattccat tcaacggtgg
    10861 tattattatc aatatctatc ccggcacctt tataagactt atcttttccg tacactttat
    10921 atgtctggtt cttatcgaat ataagcccgc caatgccaag atcggtatcc tgcttgagcg
    10981 ttaatacccc tcctcctgag acagacaggt ccttatcttt tataatcgat gataactctt
    11041 gttttttacc atccagctgc atagtgtttg tgaaattctt ttttaaattg tttaccagaa
    11101 tattattaaa taacgagtag tttgtccagg ctatattggt actactgata aaaccaactg
    11161 tactggtaac accatggaaa acccattttt gttgttcttt atcatataga taagaaccag
    11221 aaccactatc gccaaaaatt ggtgagtttt taaaaggggt tctgttggta taaatgtaac
    11281 tcttgttttt ccagtccaga ttaaaaagac ttgcgcttaa caagtcggga ttatagtttt
    11341 gtccaaacag atattgtttg ccgtttaata tcgttgaggt tgttccggca cctgctctga
    11401 agccaattat ttgttcctta ccgttgtagt ttacgccata tcttttcatc gcatcttcgg
    11461 gagaaagtga gaagtcggca ctctccgtaa cccctgttgt ttccacgacg aatttattca
    11521 gacgatgaac cgaaaagtcg ccagatgata ctctgttact tgctttatat gctgtctgat
    11581 cccatgactg tgttgttatt gcatgatgct gtgtaccgtt atgagtcgca gtaacactgt
    11641 acgcacctcc aattgatgtt gttgcacctt tgttggaaac agcagaaaaa tcaggtacag
    11701 gtagttctgg aaaatgaaat atctttccat ctttcatcat taattgaaca tttgttgcac
    11761 ccggtctgaa ctctcctctg ttttgagcaa ggtcaagata atcgcgggtc catatattat
    11821 ttatatccag cattgctgca aatgctgttt cgccataact tccatatatt gaggataaaa
    11881 taataccgag gataactttc tttcttgatg taccgacact aactttccgg gtcagttcag
    11941 aaacagcaac taatccacct gtaatatgac tatatttcag tgaataaatt ttattcatac
    12001 gtgaactccg atatttctga gttgtggtta tgggatagca tttaattaca acggggaact
    12061 atcagtcatg gtacgtaact gataagggta taatgataat cactgtcagt tatatttgta
    12121 tgacagatgt aacaaaatga aaaaaaagat aagcacaagt gattgattgg ttagatattg
    12181 atcacttggt gtttgtattt tgaattttgc gatcaaaatt tgatctttgt tgtttccggt
    12241 caatccttta caggttttct gtattatccg ggggagttaa gtctgtcgat gtccgacaga
    12301 cttttgatga tggaaacaac atgcagcgat aatgtgcatg ctgttttgca ggtggaactc
    12361 agaaggtgaa ctggaactgt gcgaccgcac cgtaagtgtt gtccgtgccg atcaggcctg
    12421 caacatcaag gtgaaccaca tcaaacggtg agatccccac cccggccgtg aatgcactgc
    12481 cgtcgttacc ggccagattc tgacggtaac cggcgcgcag ctgtgcccat ttccaggcat
    12541 taaattctgt gccaatggcg gcaaactgac ggttgctgtc ggaggtgaaa ccgctggccg
    12601 gggtcagatc cacatcaaat gcagtggtga acatcgcgtt gtgccaggag acaccggccg
    12661 tcacctgcgg gcggatcctg aaggtttccg tgataccgtt cacttctttt gtctcaatac
    12721 tacgggagat caggttctgt gcgaccagtc ccagcgtcca gttgtcatcc aggtccgtac
    12781 tggcaccgat gtcggcgttg atgccatttt tcgtgttgtg gtagcggtcg cctttgaagg
    12841 cgctgctgtc ataatttctg accagcacgt tgtagttgaa caggtcgacg cgctggaatt
    12901 tgggggggaa gcccagagac catttctgcc cggccgtttc cagttccgta gccagagcaa
    12961 tacccacatc agtgatcagt gccgtgcggg cacaggcctg tgatgtcagg cggcttttgt
    13021 ccacctcctg cccggtgctg tttgccacct cttccaggta agtgaggtca gcatcagaga
    13081 ctttaccgtc gacactgacg gtgccgtgtg atttaaccat cagtgcggca gagaggcgat
    13141 caccgggaag ggccgccaca gcagaaacgc ccagctgtgc ggcggcatgt gtgttacgga
    13201 agtcccggag gtgttctttc aggcgtgcgg cagcctcagg aacaccgtgg ttactttcga
    13261 tggcgcggtc gaaggccttc cactcatcac tgatccggtc ggcgttgtcg gtgatattat
    13321 ccggattgga gagctgcgcg ccgacggacg gaagcaggat gctgggctca tgcgcgacgc
    13381 aaaatccacg atgtatatat cagtactaaa agcgcgacgg cggaagaagc tctgaaacta
    13441 atcggtgaac tgtacgccat agagcacgat atacgcgggt taccggtgtc tgaacgcctg
    13501 gcggtcaggc aaatgcagag tagaccgctc ctgacttccc tgtataagct gatgcaggag
    13561 aaagaacaca cgttatcgaa gaaatgtcgt ctgggagatg cgttccggta tatcaggaag
    13621 cactgggctg cgttgtgcaa cttctgtgat gatggcctgg ctgaggtaga taataatgca
    13681 gcagaaagag cgcttcgtgc agtctgtctc gggaagaaaa ctttttcgag gatcactcat
    13741 tccgtgtctg actgcttcag ggtttaattt tgcttctgct gactgaatga tgattgggca
    13801 gcaagtgcaa tatcccacat aaatccagtc aactcacgag ccaccgccgt aatggctact
    13861 tgtgattttt tcccttttgc tgtcagagtt cggtaacgag aacacaatcg ttgttgagct
    13921 ttccatgcaa tatctttgga atattgtgtt acagagtccg gtcggtaata tataagccat
    13981 gctccaactt ttgcaggtgt acgataagac caggcggctt cgtaaagcag acgtctcagt
    14041 tcgctgtttc caacttttgt tattcctctg ggacgaatac tgtttccact ggaatattca
    14101 cctggcacca
//
LOCUS       DAZSIO010000106        13964 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZSIO010000106 DAZSIO010000000
VERSION     DAZSIO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.106"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACHX5T_002798"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACHX5T_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR8467918.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACHX5T_002799"
     CDS             2219..2767
                     /locus_tag="ACHX5T_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR8467919.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACHX5T_002800"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACHX5T_002800"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR8467920.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACHX5T_002801"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACHX5T_002801"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR8467921.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACHX5T_002802"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACHX5T_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR8467922.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="ACHX5T_002803"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="ACHX5T_002803"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR8467923.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="ACHX5T_002804"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="ACHX5T_002804"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR8467924.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12380
                     /gene="pepN"
                     /locus_tag="ACHX5T_002805"
     CDS             9765..12380
                     /gene="pepN"
                     /locus_tag="ACHX5T_002805"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR8467925.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="ACHX5T_002806"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="ACHX5T_002806"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR8467926.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="ACHX5T_002807"
     CDS             complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="ACHX5T_002807"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR8467927.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3550 a         3563 c         3527 g         3324 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggtctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgctgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agttttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc ataggaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagagta cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac
     8221 aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcctggcg gcgaatacgg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag
    10261 gcgaactcga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt
    10381 ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatggt ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgccg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga
    12361 taactaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 taatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg
    13201 tacttttacc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc
    13501 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac
    13681 cagcccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaacagcgt
    13741 accgagcgcc accagaaaga ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagcct
    13861 ttctccccag cgcgacagtc cgctaatcag tcccagaatc aggcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgct
//
LOCUS       DAZSIO010000107        13880 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZSIO010000107 DAZSIO010000000
VERSION     DAZSIO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13880
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.107"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(1..386)
                     /locus_tag="ACHX5T_002808"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="ACHX5T_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="ACHX5T_002809"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="ACHX5T_002809"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR8467928.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="ACHX5T_002810"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="ACHX5T_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR8467929.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="ACHX5T_002811"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="ACHX5T_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR8467930.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="ACHX5T_002812"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="ACHX5T_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR8467931.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="ACHX5T_002813"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="ACHX5T_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR8467932.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="ACHX5T_002814"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="ACHX5T_002814"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR8467933.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="ACHX5T_002815"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="ACHX5T_002815"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR8467934.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="ACHX5T_002816"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="ACHX5T_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR8467935.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="ACHX5T_002817"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="ACHX5T_002817"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR8467936.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="ACHX5T_002818"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="ACHX5T_002818"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR8467937.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="ACHX5T_002819"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="ACHX5T_002819"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR8467938.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="ACHX5T_002820"
     CDS             10748..11212
                     /locus_tag="ACHX5T_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR8467939.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="ACHX5T_002821"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="ACHX5T_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR8467940.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /gene="selO"
                     /locus_tag="ACHX5T_002822"
     CDS             12236..13672
                     /gene="selO"
                     /locus_tag="ACHX5T_002822"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR8467941.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="ACHX5T_002823"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="ACHX5T_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR8467942.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
BASE COUNT         3498 a         3211 c         3693 g         3478 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgcctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat
//
LOCUS       DAZSIO010000108        13558 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZSIO010000108 DAZSIO010000000
VERSION     DAZSIO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.108"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            112..1167
                     /gene="phoE"
                     /locus_tag="ACHX5T_002824"
     CDS             112..1167
                     /gene="phoE"
                     /locus_tag="ACHX5T_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR8467943.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            complement(1206..1607)
                     /gene="crl"
                     /locus_tag="ACHX5T_002825"
     CDS             complement(1206..1607)
                     /gene="crl"
                     /locus_tag="ACHX5T_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR8467944.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="ACHX5T_002826"
     CDS             complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="ACHX5T_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR8467945.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="ACHX5T_002827"
     CDS             complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="ACHX5T_002827"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR8467946.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3721..5178
                     /gene="pepD"
                     /locus_tag="ACHX5T_002828"
     CDS             3721..5178
                     /gene="pepD"
                     /locus_tag="ACHX5T_002828"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR8467947.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5235..5849)
                     /gene="prfH"
                     /locus_tag="ACHX5T_002829"
                     /pseudo
     CDS             complement(5235..5849)
                     /gene="prfH"
                     /locus_tag="ACHX5T_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5911..6954)
                     /locus_tag="ACHX5T_002830"
     CDS             complement(5911..6954)
                     /locus_tag="ACHX5T_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR8467948.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            complement(7200..7652)
                     /locus_tag="ACHX5T_002831"
     CDS             complement(7200..7652)
                     /locus_tag="ACHX5T_002831"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8467949.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="ACHX5T_002832"
     CDS             complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="ACHX5T_002832"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFR8467950.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="ACHX5T_002833"
     CDS             complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="ACHX5T_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFR8467951.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="ACHX5T_002834"
     CDS             complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="ACHX5T_002834"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR8467952.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRECRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="ACHX5T_002835"
     CDS             complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="ACHX5T_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR8467953.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            10291..12003
                     /locus_tag="ACHX5T_002836"
                     /pseudo
     CDS             10291..12003
                     /locus_tag="ACHX5T_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(12220..12717)
                     /gene="rayT"
                     /locus_tag="ACHX5T_002837"
     CDS             complement(12220..12717)
                     /gene="rayT"
                     /locus_tag="ACHX5T_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFR8467954.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(12893..13414)
                     /locus_tag="ACHX5T_002838"
                     /pseudo
     CDS             complement(12893..13414)
                     /locus_tag="ACHX5T_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
BASE COUNT         3376 a         3517 c         3393 g         3272 t
ORIGIN      
        1 aatatcatta atctgtaata tatctttaac aatctcaggt taaaaacttt cctgttttca
       61 acggggctct cccgctgaat attcgcgcgt taattaaaat caggaatgaa aatgaaaaag
      121 agcactctgg cattagtggt gatgggcatt gtggcatctg catctgtaca ggctgcagaa
      181 atatataata aagacggtaa taaactggat gtctatggca aagttaaagc catgcattat
      241 atgagtgata acgccagtaa agatggcgac cagagttata tccgttttgg ttttaaaggt
      301 gaaactcaaa ttaacgatca actgactggt tatggtcggt gggaagcgga gtttgccgga
      361 aataaagcgg agagtgatac tgcacagcaa aaaacgcgtc tcgcttttgc cgggttgaaa
      421 tataaagatt tgggttcttt cgattatggt cgtaacctgg gcgcgttgta tgacgtggaa
      481 gcctggaccg atatgttccc ggaatttggt ggcgattcct cggcgcagac cgacaacttt
      541 atgaccaaac gcgccagcgg tctggcgacg tatcggaaca ccgacttctt cggcgttatc
      601 gatggcctga acttaaccct gcaatatcaa gggaaaaacg aaaaccgcga cgttaaaaag
      661 caaaacggcg atggcttcgg cacgtcattg acatatgact ttggcggcag cgatttcgcc
      721 attagtgggg cctataccaa ctcagatcgc accaacgagc agaacctgca aagccgtggc
      781 acaggcaagc gtgcagaagc atgggcaaca ggtctgaaat acgatgccaa taatatttat
      841 ctggcaactt tctattctga aacacgcaaa atgacgccaa taactggcgg ctttgccaat
      901 aagacacaga actttgaagc ggtcgctcaa taccagtttg actttggtct gcgtccatcg
      961 ctgggttatg tcttatcgaa agggaaagat attgagggca tcggtgatga agatctggtc
     1021 aattatatcg acgtcggtgc tacgtattat ttcaacaaaa atatgtcagc gtttgttgat
     1081 tataaaatca accaactgga tagcgataat aagctgaata ttaataatga tgatactgtc
     1141 gcggttggga tgacgtatca gttttaatga atattgccgg atgtgatgca tccggcacat
     1201 ttcactcacg ccgttaactt caccggttca tcacgaaaat cattcgccgg ttccagcttc
     1261 agattcagcg tcgtcagcag ttcacgcagc ttctcgtgaa actcacgcag ggtgtgctcc
     1321 agtcgttcaa ccacttcagt atcttttacc ggaacactct tccagtcgcc tgctttatcg
     1381 aacagaccaa actggtagct gtaggtaaaa cgggattcct gcgcttccag ctccatccac
     1441 cagccccaga attcacgcac ttccggtgcc ggtttcacgt tgacgcatac agccagacaa
     1501 tcgaaaaaga atcgattatc ttcgcactta ccttcacgaa tatacgggcc tagtgcggta
     1561 aattttttga tcaatctgct cttcgggtgt ccactcggta acgtcattgc tatctcctgt
     1621 tgtgatgcaa ctgttttacc aaattggcaa aatttagcaa atttttaaca caagcgtttt
     1681 tcgatccagc cggtgatttc ctgaagacct ttgtcaaaat tccgatacac cgggttaaat
     1741 gggatctcta ataatttacc gtcagcagat gatgaggtga ttaagcgtga gtcctcttcc
     1801 gggctgaacg gatcgttctt ccagtagcct gataacattg gcgttgggca gcgacgcccc
     1861 agcaatcctt gcacttttaa tgaatagcga ttcagctcca cgcgtaacgc ttcgtcggaa
     1921 gcatcatgca tccccaaacg actcgccaga acgtcaagat acatttccgg cacctgttgc
     1981 tggcacttaa aatcactcaa cagggtatga actaccggac caagacaggc aaccgctttc
     2041 agacgcggcg attcaaggta tgccagacgc acggcaacgt tagcgccgaa acggaaacca
     2101 aaggccgcga cgcgagtgtg atccacccac ggtacgttag gcagcgcctt taagacgtgc
     2161 tgatgcaaca ggctggagtc ctgggtgagc ttccattttg aagaaaagcc caccgacggc
     2221 atatcaatag tcagcatcgc aatgccgcgc ggcgcaaaat aacgttcata caggctgtaa
     2281 tagtccgtct gcatcgcatc cagaccacca cacattaata ctgtcgggaa cgggccatcg
     2341 cctttcggca tatgcaaaaa gccggtgatg ggcgcaccgc cgggtacggt aaactccatc
     2401 tgccgcatcg tgcccggcag acgctgggcg gcctcttcat aggcgcggtt tgataaagcc
     2461 tgcgcttgct cggccaggtc atctcctttc agatgaggat aggcggcaat gttgtacaac
     2521 gtagccgcat gcagccagtg tcgaccactg agttgcggat cttcttccgt acaggctttt
     2581 tgttgccaca ccatcgcctg ggtggcccac tcataaatcc agttgccacc acggtagcca
     2641 atcacggtat cgtataaatc atcgtcggta cgttcagcat cgctcatcac aatacgtgcc
     2701 tggacgtcga ggatttcacg tgggtcaata ccgcgccaga tccacatcag acggttaatc
     2761 atgcgatacc agtgagggat ggttttacca tcaagagccg actgcacagg cggttgtgcg
     2821 ccgtgattaa agcggcggac tagcgtcgag gtttcaggat gtttaaagcg gggtttgaac
     2881 agggtttcgc tcaggtttgc ctgtgtcatg gatgcagcct ccagaatact tactggaaac
     2941 tattgtaacc tgcctgaagt taaaaagaac aacgcccggc aatgccaggc gttgaaaaga
     3001 ttagcgaccg gagattggcg ggacgaatac gacgcccata tcccacggct gttcgatcca
     3061 ggtatcttgc gggatatcaa caacatagtc atcaaccagc ggacgaccag ccggttttgc
     3121 gaagatggtg acaaagtgcg cttttggata catttcacga atcgcaacag cagtaccacc
     3181 ggtatccacc aggtcatcaa taacgatgaa gccttcgcca tcgccttctg cgcgtttcag
     3241 cactttaagc tcgcgctggt tgtcgtgatc gtagctggaa atacaaacgg tatcgacatg
     3301 acgaataccc agttcacgcg ccagtaacgc ccccggtacc agaccgccac ggcttacggc
     3361 aataatgcct ttccattgtt cagaaggcat cagtcggctt gcgagtttac gtgcatggat
     3421 ctgcaacatg tcccaggtga cgatgtattt ttcgctcatg tgaagtgtcc cagcctgttt
     3481 atctacggct taaaaagtgt tcgaggggaa aataggttgc gcgagattat agagatctgg
     3541 cgcactaaaa accagtattt cacatgagtc cgcgtctttt tacgcactgc ctctccctga
     3601 cgcgggataa agtggtattc tcaatacata tctcgcaagc ctgtcttgtg ttgacaacat
     3661 tttctgctaa ccctgtgacc tgcaatactg ttttgcgggt gatcgacaag gagacttaac
     3721 gtgtctgaac tgtctcaatt atctccacag ccgctgtggg atatttttgc caaaatctgt
     3781 tctattcctc acccgtccta tcatgaagag caactcgctg aatacattgt tggttgggca
     3841 aaagagaaag gtttccatgt cgaacgcgat caggtaggta atatcctgat tcgtcaacct
     3901 gctaccgcag gtatggaaaa tcgtaaaccg gtcgtcttac aggcccacct cgatatggtg
     3961 ccgcagaaaa ataacgacac cgtgcatgac ttcacgaaag atcctatcca gccttatatt
     4021 gatggcgaat gggttaaagc gcgcggcacc acgctgggtg cggataacgg cattggtatg
     4081 gcctctgcac tggcggttct ggctgacgaa aacgtggttc acggcccgct ggaagtgctg
     4141 ctgaccatga ccgaagaagc cggtatggac ggtgcgttcg gcttacagag caactggttg
     4201 caggctgata ttctgattaa caccgactcc gaagaagaag gtgaaatcta catgggttgt
     4261 gcggggggta tcgacttcac ctccaacctg catttagatc gtgaagcggt tccagctggt
     4321 tttgaaacct tcaagttaac cttaaaaggt ctgaaaggcg gtcactccgg cggtgaaatc
     4381 cacgttggcc tgggtaatgc caacaaactg ctggtgcgct tcctggcggg tcatgcagaa
     4441 gaactggacc tgcgccttat cgatttcaac ggcggcacac tgcgtaacgc catcccgcgt
     4501 gaagcctttg cgaccattgc tgtcgcagct gataaagtcg acgcactgaa atctctggtg
     4561 aatacctatc aggagatcct gaaaaacgag ctggcagaga aagagaaaaa tctggccttg
     4621 ttgctggact ctgtagcgaa cgataaagct gccctgattg cgaaatctcg cgataccttt
     4681 attcgtctgc tgaacgccac cccgaacggt gtgatccgca attccgacgt ggcaaaaggt
     4741 gtggtcgaaa cctccctgaa cgtcggtgtg gtgaccatga ctgacaataa cgtagaaatt
     4801 cactgcctga tccgttcact aatcgacagc ggtaaagact acgtggtgag catgctggat
     4861 tcgctgggta aactggctgg cgcgaaaacc gaagcgaaag gcgcatatcc tggctggcag
     4921 ccggacgcta attctccggt gatgcatctg gtacgtgaaa cctatcagcg cctgttcaac
     4981 aagacgccga acatccagat tatccacgcg ggcctggaat gtggtctgtt caaaaaaccg
     5041 tatccggaaa tggacatggt ttctatcggg ccaactatca ccggtccaca ctctccggat
     5101 gagcaagttc acatcgaaag cgtaggtcat tactggacac tgctgactga actgctgaaa
     5161 gaaattccgg cgaagtaatt atttgatttg ctgccggatg gcgtttaatc gccttccggc
     5221 agtttcatcc ttcattatcc ttcgataaaa gccatccctg taaacgttcg tcgcgggttg
     5281 ccacgttcaa tctggtgatg gaacattcgc cgctgtgatt tcagcaccgc gctattttcc
     5341 tattgctgct gatccagctt ccaggcaatc agcaatcgcg ccagccgttt gttagcatgc
     5401 tggctgcgct ctgactgaac cttcacgcta ataccggatg ccaaatgcgt ggcgcgtacc
     5461 gccgagtcgg ttttattgac atgttgaccg cccggccccg acgaacgcag cgtctcatac
     5521 cggatcgcat cggattgctc ctgctcatca gcggtaaaac gcccaatgcc cagaaaccag
     5581 tttttacgcc cgtgatgtgg gcgatacggg ctcgggcaaa tccactgaat agtgccacac
     5641 cagctttcgc ttaacgccca tgcgttgtcg ccatccagag aaatcagcgc cgaacgcagc
     5701 gtgtcagagt agcggcctgt ttctgtttcc agcaccgtta ccgcgacgtc ttgtcgggca
     5761 gcttctttaa ttagcctgtc cagcgccttt ttcactgcga gacaacattc ttccggcccc
     5821 tgcgctgagg agagttatag caagatcatc cttttttccc tccactgttt ttgagcgtta
     5881 gcaccggacg cagccgcgcg acaggaataa ttaatccggc ctgccctgac attgcaccac
     5941 actttcagcc gatttatagg cttgtggcgc ttcttcgaag atgagttgtt tatcgcgaca
     6001 aattacccga ctgccaagtt cagtacgcga gagttgcgtc gctgtgtatt tcgctgccag
     6061 acgcccttta cactcggtgc gcccccattt acgcccagcc ccatgcgcca gcgaatgcaa
     6121 tgtttcctca ctcctgacgg gctgaaccag cctggagtaa tcaccgcgtg aaccgggaat
     6181 aaccaccaga ccacagtcat caggcgtggc acctttacga tgtaagaccc cttgttgatc
     6241 accgattcga cacgcgctaa caaagttatg cgccacatcc agaaccggac ttccggtggc
     6301 cttaacctgt tgcattatgc gcaaagcgat caggtggcga ttaaaacgcg caaacgccag
     6361 cgcgtcgtca ctgccttcag gcaaaccatg atgcgaaaac gaggcaatat gccgctgtaa
     6421 aatagactgc cctagccccc tcgagccgct atgaaccagc agttgcagat gctgcgcatc
     6481 cagacctgcc agtgcaaaca gttcagcgtt gatgatttga tcaacttgtt gcagttcagc
     6541 gaaatgattg ccaccaccga tagagccaag cgagctgcgc cacggatgct gtgcaaatgc
     6601 tgacggcagg ttttcctcca gccagctttc ttcagcaacg tcatccaaag ctgataatcg
     6661 cttttcaaac ttatcggcgt tgtatttgcg agcgagaata tctgtttgcc atagcgccat
     6721 gccacagccg atatcattgc cgaccagtgc tgggtaaaaa cggcctgcgg agaagaacgc
     6781 tgcgccaatc gggtagccgc gtccggggtg taaatctggc atccccacta cgcgctgcat
     6841 gttgggtaaa tttgccgtgg tgtgtaattg ttggatcgct aaactctcga tccacaggtc
     6901 atcagatgcg atggtaaata ccgcatcgga taagggacga atataattgc ccatatacca
     6961 ttccgtaaat agactcgtca tacttcaagt tgcatgtgct gcgacttgaa ttattgagag
     7021 taacaattaa aaaatcagga aatggcagac gaatccaggg caagaaaaaa agaattaatc
     7081 ctggaaaggt ctgcaaagag tcattgataa aggatttgtc atgatttacc tccttgcagt
     7141 ttgtggaata agaaaacgcg cacatactag gcgggcgtta ttttcattgc aagctggatt
     7201 taatgttgcg gtttataacg catataaaaa ttaataaacc acgctccccg gcattcaacg
     7261 cgctgctgct taactgtctg aaaaccataa cgttcaaaaa agggttttgc ggttatgctt
     7321 gcgtccaccg taagttcgga ttcagactta ataaaaggtt ttaacaaagc gctggcaacc
     7381 ccacggcggg tgtattcagg gtcaacaaat aacatatcga tatagtgttc aacgcaggta
     7441 ataaagccga ccagttttgc attaataact gcaacccgca cttgtgattt cgcgagtttc
     7501 tccttccagc gagattcgtc aatctgcgcc caggcggcaa tttgttgtag tgaataatgc
     7561 tgactgacgg tcatcgtaac cgctctaatg aaaatagcgc atagttgctg aaaatcgcca
     7621 ggctgataat ctcttatttg tatgttattc ataatataaa ttcaaaaacg catgcgaaac
     7681 gcttctgcca tttttccaat tttatgcatt tggttgttat ctcgagccag tttatgaggt
     7741 tcaggcttca gaatggcaat gagcaaccat gcttgctcat caaacgcacc ctgacaatat
     7801 accaaatgcg cttcgtcatt cgttctgctg aattggcgca actgtggcgg aaatggatta
     7861 ttcacatttg ccagatgaat atgagcaact cgctcaaatt tgattaatgg ccaggtaaac
     7921 gagtcgtcgt agagtgcatc gcgaccaaat atatctggca aaacaccgtc acgcttatag
     7981 gaaataaaat ccgccgttaa cgcatcaagt tcctctgctg taagttgcag gcgaataagt
     8041 tttgttttga atacccgcat ccttattcct taaagtcatt gaaatcatca tccgtcatat
     8101 cattaagata ttgtttagcg cggcgtgtaa tttcctctaa tcttgcagca cttggacgaa
     8161 agcgcgcctt tatgggcttt ttctcctctt gttcagcaag catgtccatt aacgcttcga
     8221 atgcgctggc acttaagaga tatcctgcgg ggcgattatt agaaagaacc gcaactggtt
     8281 gatcaataaa gtatttagct gggtttttac gtaactcagt gatattgacc gatttttcag
     8341 cgagaattcg atgcatacag tgataccctc ataataatgc acaccagaat atacataata
     8401 gtatacatca taatcccagc accagttgtc tttccatttg cgggtcaagc aacgtcacat
     8461 gcagccccac cagacgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     8521 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgggtg gtctgctgaa
     8581 aatcgtcgaa ctttaatttc accccctggc gagcaatcag taaatcaggt tttacctttg
     8641 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     8701 gatgaatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgtaac cgttcgctgt
     8761 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     8821 agcgtttaag cagaatcacc agatcacact tttgtacatc accgcaggtc cgcagcccca
     8881 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gattttttcc agtggtaagg
     8941 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctgaccgttg ggtttattca
     9001 tgtcggaggc gattttggcg agaaacttta ctggtgccac gcccgcagat gccgtcagtt
     9061 gcagctcgtt gaagatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     9121 aatggacgct atcggtgaca tcgagataag cctcatccag tgacaacggt tcaatgcgcg
     9181 aggtgtagcg cgagaagatt tcacggatat gatttgaggc ttctttgtag gcgtcaaagc
     9241 gccccggaag caaggtgaga tgtgggcata atttgagcgc catcccagta ggcatagcgc
     9301 tacgtacgcc aaatttacgc gcgggataat tggcggtgct gatcaccccc cgacattcgc
     9361 ggctgccgcc aatagcaata gggatatcgc gcagagcggg attgtcgcgc atctccaccg
     9421 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     9481 tggtaaagta tacagtgatt tcaggttttg agaaatgcgt aaagattcag catttagcgc
     9541 attcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     9601 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     9661 atctcaatac gccgattgcc cgcgctttgc ggattcttcg cgtccagcag catctggtcc
     9721 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     9781 cgagctgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtag
     9841 gccatcgcat cggtatgccc ggtaataata attttgttat agagcgagtc gaacactggc
     9901 gcgagctcca ccagcaaagt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     9961 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
    10021 tccagattcg cttccatatg cgcatcacgg gcaatggtgt tgatactggt tgccaactcg
    10081 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattcac atcacgcgct
    10141 tttttctccg tctcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
    10201 attttattta acggcgacag cccaccaccg ttaaaaatcg actgcccgtg cagcgcggcg
    10261 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat cgtcgtcacc
    10321 aaagaggccg agcgtatttc cgaggtttct gcccgcttta ccctggacgc gatgcccggc
    10381 aaacagatgg cgattgacgc cgatcttaac gccggattga tcaaccaggc gcaggcgcaa
    10441 acccggcgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
    10501 aagtttgtac gcggagacgc catcgccggg atgatgattc tggcgatcaa cctgagcggc
    10561 ggcgtctgta tcggcatctt caaatacaac ctgagcgccg acgccgcctt ccagcaatac
    10621 gtgctgatga ccatcggcga cggcctggtg gcgcagatcc cttccctgct gctctccacc
    10681 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgc
    10741 caccagttgc tggcaagccc gtcggtgctc tacaccgcca ccgggatcat gttcgtgctg
    10801 gcggtggtgc cggggatgcc gcatctgccg tttttgctgt tcagcgccct gcttggcttt
    10861 accggctggc ggatgagcaa acgcccgcag gcggcggagg cggaagagaa aagcctcgaa
    10921 acgctgaccc gcaccatcac tgaaaccagc gaacaacagg tcagttggga aactattccg
    10981 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcg
    11041 cagggcaacc cgctcaccca gcggattcgc ggcgttcggc aggtgatttc cgacggtaac
    11101 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gcctcaagcc cagccagtac
    11161 gctattttca tcaacggcat tgaggctgat gaagcggata ttccggcgga taaactgatg
    11221 gcgctgccct ccagcgaaac ctacggcgag attgacggcg tgctggggaa cgacccggcg
    11281 tacgggatac cggtcacctg gatccagcct gcgcagaaag cgaaggctct gaatatgggg
    11341 tatcaggtga tcgacagcgc cagtgtgatt gccacccatg tgaacaagat tgtgcgcagc
    11401 tatattcctg atttgtttaa ctatgatgac atcacgcagt tgcataaccg tttgtcgtcg
    11461 atggcaccgc gcctggcgga ggatttaagc gcggcgctca attacagcca attgctgaaa
    11521 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
    11581 gtgctggtcg ccagtagcac ggtgactaaa gatcatattc tgctggcggc cgatgtgcgc
    11641 ctggcgctgc ggcgcagcat tactcatccg ttcgttcgca agcaggagct gacggtgtat
    11701 acgctgaata atgagctgga aaatctgctg accaacgtag tgaatcaggc gcaacaggcc
    11761 ggcaaagtga tgctcgacag cgtgccggtt gacccgaata tgctcaacca gttccagagc
    11821 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagatccggt gctgctggtg
    11881 ccgccgcagc tacgcccttt gctgccgcgt tatgcaaggt tgtttgcgcc ggggctgcat
    11941 gtgctgtcgt ataacgaagt gccggatgag ctggagttga agattatggg ggcgttgatg
    12001 taagaatccg gcatcggtgc acaattgccg gatgcggcgt aaacgcctta tccggcctac
    12061 cgttcaggca caagttttta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
    12121 cataactgcc ggatgcggcg tgaacgcctt atccggccta ccgttaaagc acgatttgta
    12181 ggccggataa gcgtaagcgc atccggcacc agcgcacagt tataaaatga tacgctcccc
    12241 ggcacttaaa tccgttatgt ctcccgccca atcgatggga tataaccctc gcgcgacatc
    12301 gcgatggaac gttgagaatg gccaatcact cacctgcttt acccagccat gtttctctgg
    12361 atttatataa atataatcaa catgatggcg ataatctttt gtattgcgaa tggcatgctc
    12421 ccaaaaacgc ggttgccaga tatttttcaa tccgcaagca tgggtgaact gctttttaat
    12481 ctcccgccag cgagaggaaa aatcatcatc gctttcgggt aatgtccata tgcaatgcat
    12541 atgctccggc aaaacgaccc aggcgttaat ttcaaaaggt ctgtctcgct taacgttaat
    12601 aatggcgtta cggagcgtct ggaactgggt ggtcagaaga tggcttcgac gattgcgtaa
    12661 atttaccgtg aaaaaccatg ttcccccctt gatgtaataa cgacgatatt ccgacatagc
    12721 gactcctttt tctcaacggt aagaggaaaa ggaaaggccg acaatgacgc tgtgttatat
    12781 cgggaaagcg gctcgcaaaa acgcacagat ttgtaggcat gataagacgc gtcagcgtcg
    12841 catcaggcat ccgtgcacaa ccgccggatg cggcgtaaac gccttatccg gcctacaaaa
    12901 tcatctcttc cgtcaaaatc ctgcgcgcgc ctaaaaaatg gtcctgccag aaaggttcgg
    12961 ctaaccggct tacccgaatg gtttccccgg tgcgcagtga ctcaataaac tgaccgtcgc
    13021 ccagatacac ccccatatgg tcggcaatct cgcggctgtg gatatggaaa aatagcaaat
    13081 ctccccggcg caggtcgtta ttcgccacaa tcgttgcccg acggtagtgg tacatctcat
    13141 tggccgtgcg cgggagtttc gcctcaagga tcttgttgta ggcataaaaa actaacccac
    13201 tacaatcgaa gccttgatcg ggccgcgtgc cgccccagac gtaaggcttg ccaagctgct
    13261 gctccaggcg gtgaatcgcc acttcggtga tgttatgcag gtgatcgctg cttaaaaagt
    13321 ggttgttttc cgccagcgcc tgccagcgtc tgtcactcgc cttgagcggc gcaggaaacc
    13381 attctggatg ctgttttatc tgctcccggt tgtggtggcg ctggcgagta aagggagcaa
    13441 aacgcataac gaaaacacgc ctggatgcaa aaatctgccg agcaagaacg aagacatgaa
    13501 agacataaac cggctgaatg aaggtgagaa ttttaaaaga ccggaggagt aaacggga
//
LOCUS       DAZSIO010000109        13525 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZSIO010000109 DAZSIO010000000
VERSION     DAZSIO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.109"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            80..604
                     /locus_tag="ACHX5T_002839"
     CDS             80..604
                     /locus_tag="ACHX5T_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFR8467955.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            685..1359
                     /locus_tag="ACHX5T_002840"
     CDS             685..1359
                     /locus_tag="ACHX5T_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR8467956.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1375..3837
                     /locus_tag="ACHX5T_002841"
     CDS             1375..3837
                     /locus_tag="ACHX5T_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR8467957.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            3853..4886
                     /locus_tag="ACHX5T_002842"
                     /pseudo
     CDS             3853..4886
                     /locus_tag="ACHX5T_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(4968..5306)
                     /gene="rcnB"
                     /locus_tag="ACHX5T_002843"
     CDS             complement(4968..5306)
                     /gene="rcnB"
                     /locus_tag="ACHX5T_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR8467958.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5525..6355)
                     /gene="rcnA"
                     /locus_tag="ACHX5T_002844"
                     /pseudo
     CDS             complement(5525..6355)
                     /gene="rcnA"
                     /locus_tag="ACHX5T_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6477..6749
                     /gene="rcnR"
                     /locus_tag="ACHX5T_002845"
     CDS             6477..6749
                     /gene="rcnR"
                     /locus_tag="ACHX5T_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFR8467959.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6841..6937
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6972..7760
                     /gene="thiM"
                     /locus_tag="ACHX5T_002846"
     CDS             6972..7760
                     /gene="thiM"
                     /locus_tag="ACHX5T_002846"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR8467960.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7757..8557
                     /gene="thiD"
                     /locus_tag="ACHX5T_002847"
     CDS             7757..8557
                     /gene="thiD"
                     /locus_tag="ACHX5T_002847"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR8467961.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8622..9439
                     /locus_tag="ACHX5T_002848"
                     /pseudo
     CDS             8622..9439
                     /locus_tag="ACHX5T_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
     gene            9491..10237
                     /locus_tag="ACHX5T_002849"
     CDS             9491..10237
                     /locus_tag="ACHX5T_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR8467962.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10211..11176)
                     /locus_tag="ACHX5T_002850"
     CDS             complement(10211..11176)
                     /locus_tag="ACHX5T_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR8467963.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11173..12176)
                     /locus_tag="ACHX5T_002851"
                     /pseudo
     CDS             complement(11173..12176)
                     /locus_tag="ACHX5T_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12216..13268
                     /gene="fbaB"
                     /locus_tag="ACHX5T_002852"
     CDS             12216..13268
                     /gene="fbaB"
                     /locus_tag="ACHX5T_002852"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR8467964.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
BASE COUNT         3590 a         3122 c         3394 g         3419 t
ORIGIN      
        1 cttttttgat gtgagaaatt tgtttccaag agatacagac tcttaacaac caaaaataaa
       61 ttttattaag gaacatatta tgaaacgttc aattattgct gccgctgtct tttcttcttt
      121 ttttatgagc gctggagtat ttgctgcaga cgttgatacc ggaacattaa ccattaaagg
      181 aaatattgca gaatctccgt gtaaattcga agcgggtggt gattcagtaa gtattaatat
      241 gccgactgta ccaaccactg tctttgaagg taaagctaaa tattctacct atgatgatgc
      301 agtcggtgta accagcagca tgttaaaaat tagctgcccg aaagaagttg ctggtgtaaa
      361 actctcgttg atcacaaacg acaaaataac cggtaacgat aaggcgatag ccagtagcaa
      421 cgataccgta ggtgataaca gcgatgtcct agatgtttct gcacctttta acattgagag
      481 ttataaaaca gcggaaggtc aatatgctat tccgtttaaa gcaaaatacc tgaaactgac
      541 agataactca gtgcaatcag gtgatgtgtt atcttctctg gttatgcgtg tggcgcagga
      601 ttaataaata aaattgcaag tttctccagg taataaacat ggccgctatc cccatggcgg
      661 ctttttaatt taagaggcat taagatgaaa ggattattat ctttactcat tttttctatg
      721 gtccttcctg cacatgccgg aattgttatc tacgggacgc gcattattta cccggcagaa
      781 aataaagaag ttatggtgca gttgatgaac cagggaaatc gttcttcgct gctgcaggcg
      841 tggattgatg atggcgatac gtcgttacca ccagaaaaaa ttcaggttcc tttcatgtta
      901 acgccaccag tggcaaaaat aggggcaaat tccgggcagc aagtaaaaat caaaattatg
      961 ccgaataaac tgcccactaa taaagaaagc attttttatc tgaatgttct gtatattcca
     1021 ccaaatagtc cagagcaaga aggcaagaat gcactgaagt ttgcgatgca aaacagaatt
     1081 aagttgtttt accggccagc gggtattgct ccggtaaata aagcgacctt taaaaagtta
     1141 ctggtaaatc gcagtggcaa tggattggtg ataaaaaatg actcagctaa ttgggtgacg
     1201 atttcggatg tcaaagctaa taatgttaaa gtcaattatg aaactattat gattgccccc
     1261 ttagaaagtc agagtgttaa tatcaaaagt aataatgcaa ataactggta tctgaccatt
     1321 atcaatgacc acggcaacta tattagtgac aaaatttaat tgcaggagct gcctatgttg
     1381 agaatgaccc cacttgcatc agcaatcgta gcgttattgc tcggcattga agcttatgca
     1441 gctgaagaaa cctttgatac ccattttatg ataggtggaa tgaaagacca gcaggttgca
     1501 aatattcgtc ttgatgataa tcaaccctta ccggggcagt atgacatcga tatttatgtc
     1561 aataagcaat ggcgcgggaa atatgagatt attgttaaag acaacccgca agaaacatgt
     1621 ttatcaagag aagttatcaa gcggttaggc attaatagcg ataacttcgc cagcggtaag
     1681 caatgtttaa catttgagca acttgttcag ggtgggagct atagctggga tatcggggtt
     1741 tttcgtctcg atttcagtgt cccgcaggct tgggtggaag aactggaaag tggctatgtt
     1801 ccaccggaaa actgggagcg gggtattaat gcgttttata cttcttatta tgtgagtcag
     1861 tattacagcg actataaagc gtcgggtaac aacaagagta catatgtacg ttttaacagc
     1921 gggttaaatt tactggagtg gcaactgcat tctgatgcca gtttcagtaa aacaaataac
     1981 aatccagggg tgtggaaaag caataccctg tatctggaac gtggatttgc ccaatttctc
     2041 ggcacgcttc gcgtgggtga tatgtacaca tcaagcgata tttttgattc tgttcgcttc
     2101 agcggtgtgc ggttgtttcg tgatatgcag atgttgccta actcgaaaca aaattttacg
     2161 ccacgggtgc aggggattgc tcagagtaac gcgctggtaa ctattgaaca gaatggtttt
     2221 gtggtttatc agaaagaggt tcctcctggc ccgtttgcga ttacagattt gcagttggcc
     2281 ggtggtggag cagatcttga tgttagcgtg aaagaggcgg acggctcggt aaccacctat
     2341 ctggtgcctt atgcagcggt gccaaatatg ctgcaacccg gcgtgtcgaa atatgatttt
     2401 gcggcggggg cgagcaagca aagtgatttt gtccaggcag gttatcagta tggttttaat
     2461 aacttattga cgctgtatgg cggcacgatg gttgctaata attactatgc gtttaccctc
     2521 ggaacgggtt ggaacacgcg cattggcgca atttccgtcg atgccacgaa gtcgcatagt
     2581 aaacaagaca acggcgatgt gtttgacggg caaagttatc aaattgccta caacaaattt
     2641 gtgagccaaa cgtcgacgcg ttttggtctg gcggcctggc gttattcgtc gcgtgattac
     2701 cggacattta acgatcacgt ttgggcaaac aataaagata attatcgccg tgatgaaaac
     2761 gatatctatg acattgccga ttattaccag aacgattttg gccgtaaaaa tagcttttct
     2821 gccaatatga gccagtcatt gccagaaggc tggggttctg tgtccttaag tacgttatgg
     2881 cgagattact gggggcgtag cggcagcagt aaggattatc agttgagtta ttccaataac
     2941 tggcggcgga taagctatac cctcgcggca agccaggcgt atggcgagaa tcatcatgaa
     3001 gagaaacgtt ttaatatttt tatatcaatt ccctgtgatt ggggtgatga cgttacgacg
     3061 cctcgtcggc aaatatatat gtctaactca acgacgtttg atgatcaggg ttttgcctca
     3121 aataatacgg gattatcagg aacagtaggg agtcgggatc agttcaatta tggtgtcaac
     3181 ctgagtcatc aacatcaggg aaatgaaacg acagctgggg cgaatttgac ctggaacgcg
     3241 ccggttgcga cagtgaatgg cagttatagt cagtcgagta cttatcgaca gactggagcc
     3301 agtgtttcag ggggcattgt cgcctggtcg ggtggcgtta atctggcgaa ccgtctttcc
     3361 gaaacgtttg ctgtgatgaa tgcgccagga attaaagatg cttatgtcaa tgggcaaaaa
     3421 tatcgcacaa caaaccgtaa tggagtggtg gtatacgacg gaatgacacc ttatcgggaa
     3481 aatcacctga tgctggatgt gtcgcaaagc gatagcgaag cagaattacg tggcaaccgg
     3541 aaaattgccg ccccttatcg cggcgcggtt gtactggtta attttgatac cgatcagcgc
     3601 aagccctggt ttataaaagc gttaagagcg gatggtcaac cattaacgtt tggttatgaa
     3661 gtcaatgata tccatggtca taatattggc gttgtcggcc agggaagcca gttatttatt
     3721 cgcaccaatg aaataccgcc atcggttaat gtagcaattg ataagcaaca aggactttca
     3781 tgcacaatca ccttcggtaa agagattgat gaaagtagaa attatatttg ccagtaatag
     3841 tcaggtggtt ctatggaaat tcgcataatg ctatttatat taatgatgat ggttatgcct
     3901 gtgagctatg cggcatgtta tagtgagtta tctgttcagc acaatttggt tgttcagggg
     3961 aattttgcac ttactcaaac acaaatagcg acatatgagc ataattttaa tgattcgtca
     4021 tgcgtgagta caaatactat cacccctatg agcccatcgg atattattgt tggactttat
     4081 aacgatacca ttaaattaaa tttacatttt gaatggacca ataaaaacaa catcacgttg
     4141 tcaaataatc agagcagttt caccagtggt tattcggtaa cggtgacacc tgcggccagt
     4201 aatgcaaaag tgaatatttc tgcggggggc ggtggttcag tgatgattaa tggtgttgcg
     4261 acattatcca gtgcttcatc atcgacacgc gggagtgccg cagtacaatt tctactgtgt
     4321 ttattaggtg gcaagtcatg gggtgcatgt gtaaatagct acagaaatgc attggcacaa
     4381 aatgcaggtg tctattcctt caatctgaca ttgtcataca acccgataac cacaacctgc
     4441 aaacaggacg atttattaat tactttagac agtattcccg ttttacaatt accagccaca
     4501 ggtaacaaag caacaataaa tagtaaaaaa agggatatta ttctgcgttg taaaaattta
     4561 ttaggtcaac aaaatcaaac atcacggaaa atgcaggtgt atttatcaag ttctgacttg
     4621 ttaaccaaca gcaacacaat actgaaaggt gcggaagata atgcgtagga tttattcttg
     4681 aaagtaatgg ttcgccagtc acacttttaa atatcactaa cagcagtaaa ggatacacaa
     4741 atttaaaaga aattgcggca aagtcaaaac ttacagatac aacggtttca attccgataa
     4801 cagccagtta ctacgtctac gatacaaaca aagttaaatc tggcgcactg gaggcaaccg
     4861 cattaatcaa cgtgaaatac gactaataac aagcaaaacg agcaagtggc taataataaa
     4921 aaataacaag gtgtctgcat ttccccctca tgatgagggg gcttttttta gcgatgataa
     4981 aaaatctcac cgtcgtaggc tttaatgatt ttaccgtcgg tgtcgctgat caacacgtac
     5041 gcgccaccca tataggtcca gtgcgtcccg gcatcaggcg cgggcaggtt acgtaactgc
     5101 cactgcttaa tgttgtactc atcggtgcgg tacatctctg ggacggtatc acctggtttg
     5161 aaatgcttaa agtcagcaat gaaactactt aactcatact tagaaattcc cgaggtattg
     5221 gtcgaacccg cggtggctgg tgcggcccag gcggcactgg taaccagcaa aagcacaccc
     5281 agcaacatct tatttttaat ggtcataaac tctccacttt ggggatctgt aatatcagtc
     5341 gcatatcttt cctgtcatac aatataactg acaagcgata ttttgttagt gatcccatga
     5401 tttcgtaaaa agtgtaaatg aaaggatgcc ctaaaagcat ccttagcgag cagagagtca
     5461 gcggaaatag ctgatttaag aatcatcctg ggctgaagat aacatgcaag cctaaaggat
     5521 tgagttatcg cattatgccc atgaagccat gtacgcccat atacacaccg actaagccaa
     5581 tcaacagact ggaaaaatag ggggcgcgtt tagcgagagt gttaaatccg ctccagcgtt
     5641 ttgcgacctg ctgaacgctg attgctgcgc caacgcctac ggtgacaagc gttaacgcca
     5701 ggccaatgct gaaactgacg accagtgttg cgcccagtgt cagggctttc aactgaatgc
     5761 atatcaacag cacggtaatt gctgccgggc aggggataaa gccaccggtt aagccaaata
     5821 acaaaatttg ccagttgatg acctctctac catcaaagcg tcgtttaatg tcattggcat
     5881 gggctcgtgc atgggcatcc tgatactcgc catgttcgtg atggtgatga tgtccttggt
     5941 cgtggtgatg ttcgtgatcg tgatgatggt gatgatgctc ataatcatgc ccgtgcatat
     6001 tctccagcca gttgcgttcg ccgcgctagg tacgccagaa catccagaac gcggtgccaa
     6061 taatgatcac tgcggaaatc agctggagcc acggttctgc tgattgagca gtaaagcgct
     6121 tactgatcac catcccgcca aaggcaatca accagaccac cgcggtatgc gaaatggttg
     6181 ctgccagtcc gagcatcacc gcctgtttaa tggtgccttt gatggcgatg ataaacgccg
     6241 ccatcatcgt ttttgagtgc cctggttcca ggccatgaag cgcaccaagt aagatggcgc
     6301 tggggatgaa gaaccaggcg tttccttgct gaagaagagt tgtaaattcg gtcatgataa
     6361 taattcttag tattaattcg gcaatctgat tctactcccc cccagtacct gatactaccc
     6421 ccccagtaga ttaatagtgc tatgattttt catattcttg ttaaccaggt gttgccatgt
     6481 ctcatacaat ccgtgataaa cagaaactga aagcgcgtgc cagtaagatt cagggccagg
     6541 tcgtggcgct caagaaaatg ctcgacgagc cgcacgaatg cgctgcagtt ttacaacaga
     6601 ttgctgctat ccgtggagcg gtaaacggtc tgatgcggga agtgattaaa ggtcatctga
     6661 cggaacacat cgttcaccag ggggatgagc taaaacgtga agaagatctg gatgtcgttc
     6721 tgaaggtgct ggattcatat atcaaataat ttattaacgc gattgtaaaa ctgccgtttt
     6781 tcctcgttta caacgggggc gctggacatt accatcctcc tctgcgattt atcatcgcaa
     6841 ccaaacgact cggggtgccc ttctgcgtga aggctgagaa atacccgtat cacctgatct
     6901 ggataatgcc agcgtaggga agtcacggac caccaggtca ttgcttcttc acgttatggc
     6961 aggagcaaac tatgcaagtc gacctgctga gttcagcgca atctgcgcac gcgttacacc
     7021 tttttcacca acattcccct cttgtgcact gcatgaccaa tgatgtggtg caaaccttta
     7081 ccgccaatac cttgctggcg ctcggtgcat cgccagcgat ggttatcgaa accgaagagg
     7141 ccagtcagtt tgcggctatc gccagtgcct tgttgattaa cgttggcaca ctgacgcagc
     7201 cacgcgcaca ggcgatgcgt gctgccgttg agcaagcaaa aagctctcaa acacgctgga
     7261 cgcttgatcc agtagcggtt ggtgcgctcg attatcgccg ccatttttgt catgaacttt
     7321 tatcttttaa accggcagcg atacgtggta atgcttcgga aatcatggca ttagctggca
     7381 ttgctaatgg cggacgggga gtggatacca ctgacgccgc agctaacgcg atacccgctg
     7441 cacaaacact ggcacgggaa actggcgcaa tcgtcgtggt cactggcgag gtggattatg
     7501 ttaccgatgg acatcgtatc attggtattc acggtggcga tccgttaatg accaaagtgg
     7561 taggaactgg ctgtgcatta tcggcggttg tcgctgcctg ctgtgcgtta ccaggcgata
     7621 cgctggaaaa tgtcgcatct gcctgtcact ggatgaaaca agccggagaa cgcgcagtcg
     7681 ccagaagcga ggggccaggc agttttgttc cacatttcct tgatgcgctc tggcaattga
     7741 cgcaggaggt gcaggcatga aacgaattaa cgctctgacg attgccggta ctgatccgag
     7801 tggtggtgcg gggattcagg ccgatcttaa aaccttctcg gcacttggcg cttatggttg
     7861 ctcagttatt actgcactgg tggcgcaaaa tacccgtggc gtacagtcgg tgtatcgcat
     7921 tgagcctgat tttgtcgccg cccagctcga ttcggtgttc agcgatgtgc gaatcgatac
     7981 cactaaaatc ggtatgttgg cggaaaccga tattgttgaa gcggtggcag aacggttgca
     8041 acgttatcag atccaaaacg tggtactcga caccgttatg ctggcaaaaa gcggcgaccc
     8101 gctgctttca ccttcggcgg ttgctacgct gcgcagtcga ttattgccac aggtttcatt
     8161 aataacgcca aacttgcccg aagctgccgc cttgctcgac gcgccacacg cgcgcaccga
     8221 acaggaaatg ctggaacaag ggcgatcgct gttggcgatg ggctgtggcg cagtgctaat
     8281 gaaaggtggt catctggatg atgagcaaag cccggactgg ctgtttaccc gcgagggtga
     8341 acaacggttt accgcaccgc gcattatgac caaaaacacc cacggcactg gttgtacact
     8401 ctctgcggcg ttggctgcac tacgcccgcg ccatacaaac tgggctgaca ccgtacagga
     8461 ggcaaaaatc tggctttcat cggcgttagc ccaggccgac acgctggaag ttggtcacgg
     8521 tattggtccg gttcaccact tccacgcctg gtggtgagca ccgtactata gctgctttgg
     8581 ttattgtcag cctttggcac tgacattcag ggatgaaacc aatgcagcta agaattacca
     8641 gtcgaaaaaa actcaccgct ctattatgcg ctcttgggct aatttccatc gttgcaattt
     8701 atccgcgtca gacggtgaat tttttctatt cgacagcagt tcagattaca gactacatcc
     8761 acttctacgg ttatcgcccg gttaaatctt tcgctattcg cattcctgcc agttacacca
     8821 ttcacggatt agatgtttcg cgctggcagg agcgtatcga ctggcagcgg gtggcaaaaa
     8881 tgcgcgacaa cgatatccgc ttacagtttg cttttattaa ggctacggaa ggcgaaaagc
     8941 tggtggaccc ctatttttcg cgtaactggc aattaagccg cgaaaatggc ctgctgcgcg
     9001 gggcgtatca ttatttctcc ccgtcggtat ccgcttcagt tcaggcgcga ttatttctgc
     9061 aaacggtgga tttctcacaa ggcgatttcc ctgccgtgct ggacgtagaa gaacggggaa
     9121 aattatcggc aaaagaatta cgcaagcggg taagtcagtg gctaaaaatg gtcgaaaaac
     9181 gcacgggaaa aaagccgatt atttactcag gagccgtttt ttatcacacc aatctggcgg
     9241 gctatttcaa tgaatatccg tggtgggtgg ctcactatta tcaacgtcgt ccggacaatg
     9301 acggcatggc ctggcgcttc tggcagcatt ccgaccgtgg acaggtagat ggcatcaatg
     9361 gtccggtgga ttttaatgtg tttaatggca cggggatgag ttgcaggcat tcgttgatgg
     9421 gattaaagaa acgccttaaa taagaagcag tgatatactc ccaccagact ccctggcagg
     9481 aaaaagacaa atggaacaag cgcataccca gctgattgcc caactgaacg agcgtatttt
     9541 agcggcggat aacacgccgc tttatattaa gtttgccgaa acggtaaaaa atgccgtgcg
     9601 cagcggggtg ctggagcatg gcaatatttt gcccggtgag cgtgatttaa gtcagttaac
     9661 cggcgtttcg cgcattacgg tgcgcaaggc gatgcaggcg ctggaagaag agggcgtggt
     9721 gacgcgttcg cgcggttacg gcacgcagat caacaacatc ttcgaatact cgttgaaaga
     9781 agcgagaggt ttttctcagc aggtggtatt gcgcgggaaa aagcccgata cgctatgggt
     9841 taataagcgt gtcgtgaaat gtcccgaaga agttgcgcag cagctggcgg ccgaagcggg
     9901 aagtgatgtc tttttgctta agcgtattcg ctatgtcgat gaagaagcgg tatcgattga
     9961 ggaatcgtgg gttccggcgc atttaattca tgatgttgat gccatcggaa tttctcttta
    10021 tgactatttc cgcagccagc acatttaccc acaacgtacg cgttcccgcg ttagcgcccg
    10081 gatgccggat gccgagtttc agtcacatat tcagttagat agcaaaatac cggtgctggt
    10141 gatcaagcaa gtggcgcttg accaacagca acggcctatt gagtacagca ttagccactg
    10201 tcgcagcgat ctatacgttt ttgtgtgcga ggagtagttc ctcgcgcgtt ggcgcacaat
    10261 caccgccccg atgcccgaca acccacgacg ccactgcatt gcccagtaat acggcatccg
    10321 ccagtggcag accagaggcc agcccggcaa gtacgccacc tgcatgactg tcgcccgccc
    10381 caatggtgtc tacaacttgc gttggaaatg ccggaatgca gccagaagcg tcgttgctga
    10441 aataccatgc gccttcttta tcgagacgaa cgatcaacgg cgcggcaaat ttctcctgcc
    10501 attgcttgcc aagtgttgtt atctctgcgg ataaagcaaa acgttcggcg gcaatctcag
    10561 cctcttgacg attgagtgac actaaaggtc gacaggccat gatccgcgcc agtaatgcgt
    10621 ccgggatatc gccaatacgt gggcaaaaat cgataaacgg cgtcacgtct tgcagctttt
    10681 ccagccattc cactaacaat tcgccgcagg gcgaggccag ttgataaccg gaaaaataga
    10741 gcaggttgcc aggcgcaacg gttaatcgtg ccaaccattg gcgattccat tgattttcca
    10801 caccgctgaa tgacataaaa gtgcgttcgc catccggctc aaccagtgcc agacaccagc
    10861 cgttatcgct ttcggcgtta tcgatcaggc tgattaaccc ctctttcgcc atccggttgc
    10921 gaataatctc cgcccacacg ccctgaccga gcggcaaggc attacccgct tcgatgccga
    10981 ggcgctttaa cgccacggca atattcagtg cgcagccgcc aacgttaacg ctctgctgtt
    11041 tcagttcgat atcgcaccca cgccagggga gggcataagc gtcggcgatc acgtcaatga
    11101 ccgccgcgcc gacgaccatc accggctgac gcgtggttaa ttcaggcagc agtgtgtgta
    11161 atcgagcgcc gctcataccg cctcccgttg ttgacgatat ttcgccagcg ctgtggcata
    11221 gcggttgaaa tcaagctgat ttaccgcatc cagctccgcc tttaacgcag gatcgacagc
    11281 gttaatgcca tgcaacgcgc cgcaaatcgc cgtcgccata gcacctatgg tgtctgtgtc
    11341 gccgccaaga ttggcgcaca ggacggcgca gcggtttggg tcggtttgtg ccagttcaac
    11401 tagcgcaatg gcgcacggaa cggactcaat agtgctggtg cctgcgccaa cgacctggta
    11461 aagctgttcg ctggcggatt cggtgccgtc ggcattacgc acaattttca gcgcaatctc
    11521 cagacgtgcc gccagtgagg cgctgaacgt ggtggtgcgt ttttgctgtg catgtcgcgc
    11581 aattgaaggc aatgaatcaa caatcgctga ccagcgttct ccgtcaatgg cacgagaaat
    11641 cgcccatgcg atgactaccg cgcctgcaac cgccagatcg gatttatggg tcgggctgga
    11701 cgccagcgcc acatcatcaa taaaggaatc aacatcacgc gctggaagca aacaacctaa
    11761 tggcgagacg cgcatcgctg cgccgttggt tacgccgttg ttttccagtt cagcaacggg
    11821 tttaccgtcg cgaatggcgt taagcgcaat cttcgaggtc ggacctagta cgtttttgtt
    11881 aaaggcgtcg aaacgcaacg cccagtcgag aatattacgc ccaatcagat tcggatcgat
    11941 cttgccttca cattccagta acgcatccgc cagacacagg ccatcgaggt atcgtcggtg
    12001 aattcggcgc ggttaaaata acaggccgcg ttattctcct ttggtccagg cagaaaacgg
    12061 tcaatccagc caaagtgtgc tttaacgcgg ctgcgtggcc aaagctcgga gggcatcccc
    12121 atcgcatccc ctaacgcctg cccataaaga gcaccgagaa tacgttctgt tttcatttaa
    12181 cttccccttg aatcggaatt tacgggagca tagtaatgac agatattgcg cagttgcttg
    12241 gcaaagacgc cgacaacctt ttacagcacc gttgtatgac tattccttct gaccagcttt
    12301 atctccccgg acatgactac gtagaccgcg tgatgattga caataatcgc ccgccagcgg
    12361 tgttacgtaa tatgcagacg ttgtacaaca ccgggcgtct ggctggcaca ggatatcttt
    12421 ctattctgcc ggttgaccag ggcgttgagc actctgccgg agcttcattt gctgctaacc
    12481 cgctctactt tgacccgaaa aacattgttg aactggcgat cgaagcgggc tgtaactgtg
    12541 tggcatcaac ttacggcgtg ttggtgtcgg tatcgcggcg ctatgcgcat cgcattccat
    12601 ttctcgtcaa acttaatcac aacgagacgc taagttaccc gaatacctac gatcaaacgc
    12661 tgtatgccag cgtggagcag gccttcaaca tgggcgcggt agcggttggt gcgactatct
    12721 attttggctc ggaagagtca cgtcgccaga ttgaagaaat ttctgcggcc tttgaacgcg
    12781 cgcacgagct gggcatggtg acagtgctgt gggcctattt gcgaaactcc gcctttaaga
    12841 aagatggcgt tgattaccat gtttccgccg acctgactgg tcaggcgaac catctggcgg
    12901 caaccatagg tgcagatatc gtcaaacaaa aaatggcgga aaataacggc ggctataaag
    12961 cgattaatta cggttatacc gacgatcgcg tgtacagcaa gttaaccagc gaaaacccga
    13021 ttgatctggt gcgttatcag ttagctaact gctatatggg ccgggctggg ttgataaact
    13081 ccggcggtgc tgcaggcggt gaaactgacc tcagcgatgc agtgcgtact gcggttatca
    13141 acaaacgcgc aggcggaatg gggctgattc ttggacgtaa ggcgttcaag aaatcgatgg
    13201 ctgacggcgt gaaactgatt aatgccgtgc aggacgttta tctcgatagc aaaattacta
    13261 ttgcctgata cgcctcatac gaacagcacc accccgtagg acggataaga cgtttacact
    13321 gcatccggca acctatgcct gatgcgacgc ggaagcgtct tatcatgcct acatagcact
    13381 gccacgtatg tttacaccgc atccggcata aaaacacgcg cactttgcta cggcttccct
    13441 atcgggaggc cgtttttttg cctttcactc ctcgaacaat tttcatattg ttgtttttgc
    13501 gatcattatc tcgacattta aaagc
//
LOCUS       DAZSIO010000110        13194 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZSIO010000110 DAZSIO010000000
VERSION     DAZSIO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13194
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.110"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..224)
                     /locus_tag="ACHX5T_002853"
     CDS             complement(<1..224)
                     /locus_tag="ACHX5T_002853"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8467965.1"
                     /translation="MPEQVITKAPYIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHSKPLEIIKT"
     gene            161..1660
                     /gene="pitB"
                     /locus_tag="ACHX5T_002854"
     CDS             161..1660
                     /gene="pitB"
                     /locus_tag="ACHX5T_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR8467966.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1709..2401)
                     /locus_tag="ACHX5T_002855"
     CDS             complement(1709..2401)
                     /locus_tag="ACHX5T_002855"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR8467967.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2575..3288
                     /locus_tag="ACHX5T_002856"
     CDS             2575..3288
                     /locus_tag="ACHX5T_002856"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR8467968.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            3320..4078
                     /locus_tag="ACHX5T_002857"
     CDS             3320..4078
                     /locus_tag="ACHX5T_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8467969.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            4124..5473
                     /locus_tag="ACHX5T_002858"
     CDS             4124..5473
                     /locus_tag="ACHX5T_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR8467970.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5473..6297
                     /locus_tag="ACHX5T_002859"
     CDS             5473..6297
                     /locus_tag="ACHX5T_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8467971.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            6309..6869
                     /locus_tag="ACHX5T_002860"
     CDS             6309..6869
                     /locus_tag="ACHX5T_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR8467972.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6900..7970
                     /gene="lptF"
                     /locus_tag="ACHX5T_002861"
     CDS             6900..7970
                     /gene="lptF"
                     /locus_tag="ACHX5T_002861"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR8467973.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            7967..9046
                     /gene="lptG"
                     /locus_tag="ACHX5T_002862"
     CDS             7967..9046
                     /gene="lptG"
                     /locus_tag="ACHX5T_002862"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR8467974.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9082..10253)
                     /locus_tag="ACHX5T_002863"
                     /pseudo
     CDS             complement(9082..10253)
                     /locus_tag="ACHX5T_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(10253..10501)
                     /locus_tag="ACHX5T_002864"
     CDS             complement(10253..10501)
                     /locus_tag="ACHX5T_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR8467975.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10534..11448)
                     /locus_tag="ACHX5T_002865"
     CDS             complement(10534..11448)
                     /locus_tag="ACHX5T_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR8467976.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11445..13133)
                     /locus_tag="ACHX5T_002866"
     CDS             complement(11445..13133)
                     /locus_tag="ACHX5T_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFR8467977.1"
                     /translation="MRYADFPTLVDALDYAALSRAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPSDRCVSWQPFYHDMG
                     LVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
BASE COUNT         3161 a         3237 c         3429 g         3367 t
ORIGIN      
        1 gttttgatta tttctaaagg ttttgaatga atgcttattg tctgatacac aagaaataac
       61 actcttttta tcgttaaaaa atgatatttc actttgccca tgccgttaaa gtcactgata
      121 atgcgtccgt tcgtaaattc aaaatggcgt aatctaatat atgctaaatt tatttgttgg
      181 ccttgatata tatacggggc ttttgttatt acttgctctg gcatttgtgt tgttctacga
      241 agcaatcaat ggttttcatg acacggcgaa tgcggtggca accgttattt atactcgtgc
      301 catgcagcca caacttgctg tggtgatggc ggcatttttt aacttttttg gcgtgttatt
      361 gggcggactt agcgttgcct atgccattgt ccatatgtta ccaatcgatt tgttgctgaa
      421 tatggggtca actcacggtt tggcgatggt cttttccatg ctgctggcgg cgattatctg
      481 gaacctggga acgtggttct ttggtttacc ggcctccagt tcgcacacct tgattggtgc
      541 gattatcggc atcggtttaa ccaacgcgct gttaaccggc tcatcggtga tggatgcgtt
      601 aaacctgcgt gaagtaacca aaattttctc ctcgctgatt gtttccccta tcgtcggcct
      661 ggtcattgcg ggtggtctga ttttcctgct gcgccgtttc tggagtggga cgaaaaagcg
      721 tgaccgtatt caccgcattc cggaagatcg caaaaagaaa aagggcaaac gtaaaccgcc
      781 attctggacg cgtattgcgc tgattgtttc cgctgcgggc gtggcgtttt cgcacggcgc
      841 gaacgacggg cagaaaggga tcggcctggt aatgctggta ctggtgggga ttgcccctgc
      901 tggcttcgtc gtcaatatga atgcgtccgg ctatgaaatt acccgtaccc gcgatgccgt
      961 taccaacttc gaacactacc tgcaacagca tcctgaactg ccgcagaagt tgattgcgat
     1021 ggaacctcca ttgcctgcag catcgactga tggcacgcaa gtaacagagt ttcactgtca
     1081 tccggcaaat acctttgatg cgattgcgcg cgttaaaacg atgctgccag gcaatatgga
     1141 aagttacgag ccgttaagcg tgagtcagcg cagccagctg cgccgcatta tgctgtgcat
     1201 ctccgatacc tccgcgaagc tggcgaaact gccaggcgtc agtaaagaag accagaacct
     1261 gctgaaaaaa ctgcgcagcg atatgttaag caccattgag tacgctccgg tgtggatcat
     1321 catggcggta gcactggcgc tcggcattgg caccatgatt ggctggcgtc gtgtagcgat
     1381 gaccatcggt gagaagattg gtaagcgcgg catgacgtat gcgcaaggca tggcggcaca
     1441 aatgacggcg gcagtgtcta tcggccttgc cagttatatt gggatgcctg tctccacaac
     1501 acacgtcctc tcgtctgcgg tagcagggac gatggtggtg gacggcggtg ggttacagcg
     1561 taaaacggta accagcatcc tgatggcgtg ggtgtttact ttaccggcgg caatttttct
     1621 ttctggtggg ctgtactgga tagcattgca gttgatttaa ccttcctgaa aatgcccggt
     1681 cctggcgatc gggcatttcc atttttgact agtgataacc acgcgcggtc ataaaatccg
     1741 taatcgcttt ttctgcatca accaacacct gttccagtgg ctgattggca tcgatatcaa
     1801 ccagttgtgc accaccaaag gttaactgtg gcgttatggc aatcttcctc gccagcgatt
     1861 cccgtttatg gtcgggttta cgtgcacagg caacttcaag gtcaacattg agtttgatga
     1921 ccagatcagg cttatggctc gccatccagt gaaacgcttt acgttcctgg cttgccagcc
     1981 atgagacaaa acgaccacct tcaacgttag gtgggaacac cgtaccatcg taagcgccag
     2041 gaatttggtc ctgaggataa cggtcggtta gaacaattaa cccgcgacga cgacaggcaa
     2101 gcatatgacg aaagcgcagt aagcgacggg cgacaaacgc tgtaattacc agcgccggaa
     2161 ctggtccagg caattttttt gctgttttca cctgatttcg ttcaattgtt ttatgtaagg
     2221 attttcccat caaaggtaat tttgtcactg cacgaccgac atttccggcc tgttttccta
     2281 aatgaactct ttcggcagca ccatattttt cgacaacggt aataagatgt tcacacaccg
     2341 ttgacttgcc tgaagcatcg ctaccaataa cggcaattaa tggaggtgtt attgactgca
     2401 tgaatatatc ctttaattta aatatccatt aaaaatattt atttggttaa tatgttttta
     2461 tgaaagcgta attcaggtca atgtcacaat tagtcattgt cacaataagg ttgaacggat
     2521 attctgagca cggaccacgt aatattcaat acattattta ctgccgggtt tttcgtgaat
     2581 caacgtaata tgtcaataat taattccacg ccagtgcgtg ttattgccat tgtaggatgt
     2641 gatggttcag gtaaatcgac cctcacggca agcctggtaa atgaactggc agcaagaatg
     2701 ccaacagaac acatttatct cgggcaatcg tccgggcgaa ttggcgaatg gatttcacag
     2761 ctccctgtta ttggcgcacc ttgtgggtgt tatctgcgaa gtaaagcggc acatgtacac
     2821 gaaaagccct taactccgcc tggcaatatt actgcgctgg ttatctatct gctttcctgc
     2881 tggcgggcgt acaagtttcg caaaatgttg tgtaaaagcc agcaaggcta tctgctcatc
     2941 accgaccgct acccgcaggt tgaagtgccg ggttttcgct ttgatggccc gcaattggca
     3001 aaaaccacgg gcggtaacgg ttggataaaa atgttaaggc agcgcgagtt gaagctgtac
     3061 caatggatgg cgtcttattt acccgtattg ttgattcgtc ttggcattga tgaacaaacc
     3121 gcgtttgcgc gtaaacctga ccaccaactg gcagcgttac aggaaaaaat cgccgttacg
     3181 ccgcaactga cattcaatgg cgccaagatc cttgaactgg atggtcggca accagccgat
     3241 gaaattatgc aagcgtcact acgcgcaatt cacgccgcgc tttcctgatc accaaaatgg
     3301 ataggggatg atttaacgaa tggatgcact acaaacacaa actgttaata gtacaaccgc
     3361 gccgcagccc aactacattc cggggctgat tgcggtggtc gggtgtgatg gcaccggtaa
     3421 atccacgctg accaccgacc tggtgaaatc gctgcaacaa cactggcaaa ccgagcgccg
     3481 ctatctgggg ctgctctccg gcgaagacgg cgacaaaatc aaacgattgc cgttggttgg
     3541 cgtctggctg gaacggcgat tggcggccaa atcctcgaaa acccaaagca tgaaaaccaa
     3601 atctccggcg ctatgggcgg cggtgattat gtactgcttc tcgctgcgaa gaatggcgaa
     3661 tctacgcaag gttcagcgac tggcgcaaag tggcgttctg gtggtcagcg atcgcttccc
     3721 gcaggctgaa atttcgggct tttattatga cggaccgggg atcggcgtcg aacgtgcgac
     3781 cgggaaaatc agcatgtttc tggcgcagcg cgaacggagt ttataccaaa aaatggcgca
     3841 ataccgtcct gaattaatca ttcgtttggg gattgatatt gatactgcca tctcccgcaa
     3901 gcctgaccat gagtatgccg agctgcagga caaaatcggc gtcatgtcga cgattggcta
     3961 taacggcaca aaaattcttg agattgattc cagagcgccc tacagcgaag tgctggaaca
     4021 ggtacagaag gcggtttctc tggttgccat cgtttctgac cgccgaagtt taacttaagg
     4081 ctggcgggat attttttact atcaacgata aggtcaggct gaattggcgg gttttaacat
     4141 caaacattgg tttgcagatg gcgcgtttcg caccattatt cgcaatagcg cctggttagg
     4201 ctccagtaat gtcgagagcg ccttgctggg gctgttggcg ctctcgtgtg ccggtaaagg
     4261 gatgacgccc gccatgtttg gtgtactggt gattgtgcaa tcgtacgcca agtcgatcag
     4321 cgattttatt aagtttcaga catggcagct ggtggttcag tacggaacgc cagccttgac
     4381 caacaataat ccgcagcaat ttcgcaatgt cgtctcattt tccttctcgc tggatatcgt
     4441 cagcggcgcg gtggcgattg tcggcggcat tgccttactg ccattccttt cccattcatt
     4501 aggtctggat gaccaaagct tttggctggc ggcgctgtat tgcacgctca ttccttcaat
     4561 ggcttcctcc acgccgaccg gcattctgcg tgcggtagat cgctttgatt tgattgctgt
     4621 acagcaggcg acgaaacctt ttctgcgcgc agcggggagc gtcgcagcct ggtattttga
     4681 ctttggtttt gtgggttttg ttattgcctg gtacgtgtcg aatctggttg gcggcaccat
     4741 gtactggtgg tttgccgcgc gcgaattacg ccgtagaaat atccataacg ccttcaaatt
     4801 gaatctgttt gagtctgccc gacacattaa aggcgcgtgg agttttgtct ggtcaaccaa
     4861 cattgcccac tccatctggt cggcgcgtaa ctcgtgcagc acagtgttgg tggggatcgt
     4921 gttaggaccc gctgccgccg ggttatttaa aatcgccatg acattcttcg acgccgccgg
     4981 aacgccagcg ggtttgctgg gtaaaagttt ttacccagag gtgatgcgtt tagatccgcg
     5041 caccaccaga ccgtggttgt taggtgtgaa gtccggttta ctggcgggcg gaatcggtat
     5101 tctggtggcg ctcgcggtgt tgatcgtcgg caagccgctc atttcgctgg tgtttggcgt
     5161 taagtatctc gaagcgtatg acctgattca ggtgatgctg ggcgcaattg tgatttcgat
     5221 gctgggcttc ccgcaggagt cattgctatt gatggcggga aaacagcgcg cttttctcgt
     5281 ggcgcaaacc atcgcctcaa tcggctacat cgtattgctg ttcatgttct gtcatctgtt
     5341 tggtgtgctg ggagcagcgt ttgcttactt cggtgggcaa tgtctggacg tggcgctctc
     5401 gttaattcca accttaaagg cattttttca gcgccattcc ttgctttata acgcagccgg
     5461 agagaaatcc tgatgatgag aaaatatttc ccgcttgagg cgagcgagcg tctatttgtt
     5521 gcgattgaag aagatgatgt cgttgatgca caggttagtt tgccgccgac tattgcacta
     5581 agttgtacca ccgaaatcat tcatgataat tacgcgttat gcctgcaatt ctggctcaat
     5641 ggcgttgatc gtcaggaatt actgcgttta gttcgcaaac aagccaaagg cgatgagctg
     5701 actgctgatg agcgcaaaca atttaaatac atgcgagcgc gctacaagca cctgcgtttt
     5761 gcgcaacggc tgtatttaaa gaagcatcag gccggatttt tattcggcaa aacgaccgtt
     5821 tttctggggc gttttcagga cggctttcgt aacggtaaga aaaatattgt gtcgtattac
     5881 ggcaacctgt tacggattta tttaagctct ccggtctggt cgttggtgaa ctattcatta
     5941 cgccatagcc agttagaaag cgtaagcagt tttattgcct atcggcaaaa gcaaatgcat
     6001 accctgaaag agattattgc caaaccgcga ctaaccggca gggaatttca tgatgtgcga
     6061 aaaatcatca gccagcaggt ttcttattac gacaccttaa gatcgcttga tcctgaaaat
     6121 aaagaggcgt tacagatttc ccgtttcctc gccgccatta atggcctgat gggagataaa
     6181 cacgacgaca tggtggcgga tgatatggaa aaccgccagt cgtattatgc accgttggcg
     6241 ctggatagcg atatttgtca gcgcttagag ctgctgattt cgcgttttcc gctatagccg
     6301 gagcaatgat gtcatctcgt ttgattattg cgttgattat catgctctta gccccgggtg
     6361 tgcaggcaca caatttcgtc actggcaaaa cggttacccc tgtttatatc caggaaggcg
     6421 gcgagctgct tctgaatagc gacgacgaaa ttcactacca aaagtggaac agcacacaat
     6481 tagcgggaaa agtccgcatt attcagtata tcgccgggcg taagtcggcg aagaaaaaga
     6541 actcactgtt aattaaagcg gttgaagcgg caaactttcc gcaggatcgt tttcagccca
     6601 ccaccattgt gaacaccgat gatgccatct ttggtactgg ctattttgtt gttggcaaaa
     6661 tcgaaaaaaa taaacgccgc tatccgtggg cgcaattcgt tattgacggc aatggacagg
     6721 gacgcgttgc ctggcgcttg ccggagcaga gttcaaccat tctggtgctg aataaagccg
     6781 ggcaaattca gtgggcaaag gacggcagcc tgacgccgga agaggtagat catgtcattg
     6841 cgttggcgca gaaactgatc aatgaataaa aaactcaaag gattgagggc gttagtgaga
     6901 tgaagctggt tgaacactac atcatgcgtg gtacgcgccg tctggtgctg attatcgtcg
     6961 gttttctgat ctttattttc gccagctact ccgcacagcg ttatctgacc gaagcggcaa
     7021 atgggacatt agctcttgat gttgtgctgg atatcgtttt ctacaaagta ctgattgcac
     7081 tggagatgtt gttacctgtt ggtctgtatg tgtcagttgg cgtgacgcta gggcagatgt
     7141 ataccgactc ggaaattacc gctatctctg cggcgggggg cagtccggga cgcttgtaca
     7201 aagccgttct ttatctggcg ataccgctaa gtatttttgt cacccttctg tcgatgtatg
     7261 gtcgtccgtg ggcttatgcg cagatttatc aactggagca gcagtcgcag tcggagctgg
     7321 atgttcgcca gttgcgggca aagaaattta acactaacga taacggacga atgatccttt
     7381 cgcagacggt tgatcaggat aataatcgcc tgactgacgc gctgatttat acttctactg
     7441 ccaatcgaac ccgtattttc cgcgccggtt cggttgatgt ggttgaccca tcacccgaga
     7501 aaccgaccgt tatgttgcac aacggcactg cctatcttct cgatcatcag gggcgtgacg
     7561 acaacgaaca gatctaccgt aatctgcaat tacatctgaa tccgctggat caaagcccga
     7621 acgtcaaacg caaagcgaaa tcggtcacgg agctggcgcg ctccgccttt cctgccgatc
     7681 atgccgaact gcaatggcga caaagccgtg gcctgaccgc attgttgatg gcgctgctgg
     7741 ccatttcatt aagtcgggta aaaccgcggc aagggcgatt ttcaacgtta ttgccactga
     7801 cgttgctgtt tattgccatt ttttatggcg gcgacgtctg ccgtacactg gtggctaacg
     7861 gcgcgattcc cctcattcct ggtttgtggt tagtgcccgg actcatgcta atgggcctgc
     7921 tgatactggt cgcacgtgac ttctctttgc tgcagaaatt ttcccgatga atgttttcag
     7981 tcgctattta atccgtcatc tctttctcgg ttttgccgcc gctgcagggc tattgctgcc
     8041 gctttttacc accttcaacc tgattaacga actggatggt gtcagcccgg gcggttatcg
     8101 ctggactcag gcggtgctgg tggtgctaat gaccttaccg cgcacactgg tcgaactttc
     8161 gccgtttatc gcgttattgg gtggtattgt tggactgggg cagttatcga aaaacagtga
     8221 gcttaccgcc attcgcagca cggggttttc tatcttccgt attgcactgg tggcgctggt
     8281 tgccgggata ttgtggactg tttcgttagg cgcgatagat gagtgggtgg cgtcgccatt
     8341 gcagcaacag gcgttgcaaa tcaaatcgac cgccaccgcg ttgggggagg acgatgacat
     8401 taccggcaat atgctgtggg ccaggcgtgg taatgaattt gtgacggtga aatcgctgaa
     8461 cgagcagggc cagccggtgg gcgtggagat atttcattat cgcgacgatc tctcgcttga
     8521 atcctacatt tatgcacgca gtgccaccat tgaagacgac aaaacgtggg tcctgcatgg
     8581 tgtgaatcat aaaaaatggc tgaatggcaa agaaacgctg gaaacattag ataatcttgc
     8641 ctggcaatcg gccttcacca gtatgaatct tgaagagtta tcaatgccgg ggaatacttt
     8701 ttctgtccgt cagcttaatc attacatcca ttatttgcag gaaaccgggc aacccagcag
     8761 cgaataccac cttgcactgt gggaaaaact ggggcaaccc atcctgaccc tggcgatgat
     8821 tttgctggct gtgccgttta cctttagtgc cccgcgttcg cctgggatgg gtagccgtct
     8881 cgctgtaggc gtcatcgtgg gcttactcac ctggatcagc tatcaaatca tggttaatct
     8941 gggattgcta tttgcgttaa gcgcacctgt taccgcgctc ggtttaccgg tagcgtttgt
     9001 gttggtggcg ttgagcctgg tgtattggta tgacagacaa cattaaaccc gccgtggcgc
     9061 aaacaccacg gcgggctcac attactgctt cagagtagca aatgcctgaa tgatggcatc
     9121 gatttgctca cgactgtggg cagcatttac gctacagcgc agcagcgtga tgcctgcagg
     9181 cgcggcaggc ggcaggataa gattcacata cacgcccggc gagataagct cgcgccagat
     9241 gcgtaaacct tcctctttcg aaccgattat tactggcact accgggctaa tatgtgaact
     9301 taactcataa cccagttttt gcagaccgtc atacagatgg ttggcgttat ccatcagctt
     9361 ttgccgcagc tctggttgtg tggcaatagt tcttaacgac gagcgcacgg tcgcaatgca
     9421 ggacggagaa ggagaggcgg taaaaatata cgggcggctg ccgtagcgca gcacttccat
     9481 cgcttccgaa acgacagcaa aaccgccaat cgaggccaga cttttactga aagtaccaac
     9541 gataatatcc acatcatctt caacgccgac agcttcagcc agtccgcgcc ccgttgcgcc
     9601 taacacgcca aacgaatgcg cttcatcgac aatcaaataa ccaccgagac gacgtttgat
     9661 atcaacaatt tccgccagcg gcgcaacgtc accgagcatg ctgtagatgc cctcaacgat
     9721 gataatcgcc tctttcgccc gttcaccgag gcgaaccatg cgccgttcca gatctttagc
     9781 atcgttatgg cggaaacgaa taatttccgc gccaccgaga gaacagacgt cataaatact
     9841 ggcgtggctg tcggcatcca gtaacaccac ggcgttatgg tcggcaagtg cgctgatgac
     9901 gcctaaatta gcggtataac cggttgaaaa acgatagcgg tagggcgatt gaaaaactcg
     9961 gcaatctctt tctctaacgc caggtgcggc gcataactgc cgtttgccat acgagaaccg
    10021 gtggtgccgg tgccttgggc cgctaacgcg gcctgacctt cgctgatggc atcatgatta
    10081 aaggttaaac cgagataatt attggtcccg gccagaatca cttttttatt gccgatccgg
    10141 ccttcggtgg cggaatacac ttcatcaata caggtgccaa acggtgttaa cccgttgtca
    10201 ctaaattgca gacgctcgcc agcaaggcgc gcatatttat cgtatagccc cattatttat
    10261 tctccagcca tggaagtaga gtttccatta attgcgcagg ggttttgaca tccagcagaa
    10321 tattaatagg aatggaaata tcgaatctgt cttccagcat cattaagaga tccatcactt
    10381 tgatcgactc caggccaaga tcgttgacga gatcgctgtc gggcttaatt tccacaccgt
    10441 tttcgaccaa atcctgtaaa caggaaagga tgtaatccat tactatttca cgatttacca
    10501 tagaaacgtt aacacacctt taaaacaaga caatcaaaat agcccgttgc gtaatgcgtg
    10561 ttccaggcta atcccgggaa accaattgat atcttcagaa atacggttat tacttgccga
    10621 ccagtcggcg tgggttaatt cacgaatttt cgagcgggtc agcataggtt ctttacctgc
    10681 caggcgactc aacgcggtac tgatatccgc aaggcctgtg agtaccggca gaggaatgtc
    10741 aaccattcgc acggaaccac aacgggcttt ggcggcaagc tgtcgtacgc gttgccagtc
    10801 atagccaccg gcgacgccat cgcataattc ataggtttgc gtctgtacag tttcggcgct
    10861 taaccactga cccactgctt gcgcaaaatc ggtgacgtga agaaaagaga gttgtgtatc
    10921 tggcgcacca agtcgtggca gcaggccgcg tagcatccag tcaaacagcg gttttaactc
    10981 tttatcgccg ggaccataca cggctgtcgg gcgaaaaacg cccagcgtaa tttcgtccgc
    11041 cattgctgcc agtcgttgct cggcgacgta tttggatttt gcataccagg agagctcggg
    11101 atggcgcgcc gccagcgaag agataaacag aaaacgttgg caaaagccgc tctcttttgc
    11161 tgcttgcatc aggcgcaggc tgccgtcaac gttacagcgg gtgaaaatct cttctttgtg
    11221 cccgcgcact tgcccggcgc aatggaccac tacgctggct ccggcaacca gctcgctaag
    11281 tgaatgagta tcttccagcg aaccgcgcac ccaggtgaga ttatcattga cgtgagcgcg
    11341 ggcagtacgc gtcaatgcgc gaacgtgaaa gccgcgggcg agcaggttat cgataatata
    11401 tttaccgata aacccggtag cgcccgtcac cgcgacagtt tgatttatgc cagggattcc
    11461 tgcacattaa gactggcagc ataagccttc tgataacgtt ttttcgcttc cgcacgggca
    11521 ggcttgccgg aggacgttcg gggaatactg tggggcggca acagatcgat agccgcggtc
    11581 acgccaaatt cgctttggat ccgtgccgcc agcgcgtgga taagctgccc gcgacgttct
    11641 tcgtcgctga tccgacactg gatctgcaaa atgatttttt cctgggcggt aacaaaagca
    11701 atcgcatcgc cagaatgaat ttccggttcc tgttccgcta tatattcaat atcctgcggc
    11761 cagatattac ggccacgaat aataatcaga tctttaatgc gtccggtgac atacagataa
    11821 ccgtccagca gataaccgag gtcgccggtg tctaaccagc ccgtcgcggc aatctcgtct
    11881 tgcgaaacct ggtcgccaaa gtaaccgctc atcagactgg gaccggagat gcaaatatgg
    11941 cctaccacac gttccgcgac cggcatacct gcttcattgc ggatttcaat accatgttcc
    12001 ggcaacgctt tgccgcagtt gacgaaagtc gatacggcgc gtgtctctgc acccggcgcg
    12061 acggctttac cctgatattc gaggatgtcg cgatccactt cgttaaccac aaccccggag
    12121 gcttcatcag agaagctgac agccagcgca ttttctgcca gtccgtagca cggcatgaaa
    12181 gttttattgt caaagttaac ctgacggaaa cattcagcga attgatggag ttgttctgcg
    12241 gagatcggct cagcaccaat accagcgacg cgccagcagg aaagatccag ttcagcgaga
    12301 tctttttcat tcacgcggcg ttggcacaat tcatagccaa acggcggcgc aacggaaacg
    12361 gtgccgcgat ttttactgat caatttaagc cattgcaaag gacgcatggc aaaatcctga
    12421 gtgcgcaaat aatctactga aagttgcgtg gcgacggggg gcaggagaaa gccgaccagt
    12481 cccatatcat ggtagaaagg ctgccaggag acgcagcggt cgctagggcg taatttaatg
    12541 ccatcgtggc ttatagcacg tagattagcc atcacttcgc gatgggtgat aatgacgcca
    12601 cggggaaaac gggtgctgcc ggaggtgtac tggaggtagg cgatatcgtt cggaactgga
    12661 cgctggagcg caacatcggc ttccggtaat gccttaaacc aagcgtggct taaaacatgt
    12721 aattcggggt tgttatgcgt cgcggcattg accagtggca accactcatc accagtgata
    12781 atggctgcgg gctggcaact tgccagtaaa ccctggagtt tggcgctcca ggaatcccgc
    12841 tgaccgacgc ccattggaat cgccaacggg acggcgacta agccagcata ctgacaggca
    12901 aaaaaagcct ctacaaaccc gctactcgtt tcggcaatca gcgccacgcg ttcgcctttt
    12961 ttcaggttca gcgataacaa ccgcttcgca ccagcttcgg cacgcgtttt taatgtctga
    13021 tattccagtt gatcttcaag ttggcaacgt ctgtcataaa aattcattcc ggctctactc
    13081 agagcggcgt agtccaaagc atcaaccagc gttggaaaat cggcatagcg cataggtagg
    13141 gaatgcgtaa agattttatt agacatatac accacgaaaa aattggatat caaa
//
LOCUS       DAZSIO010000111        13098 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZSIO010000111 DAZSIO010000000
VERSION     DAZSIO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.111"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACHX5T_002867"
     CDS             complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACHX5T_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR8467978.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="ACHX5T_002868"
     CDS             1131..1931
                     /locus_tag="ACHX5T_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR8467979.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="ACHX5T_002869"
     CDS             2009..2779
                     /locus_tag="ACHX5T_002869"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR8467980.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="ACHX5T_002870"
     CDS             complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="ACHX5T_002870"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR8467981.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="ACHX5T_002871"
     CDS             complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="ACHX5T_002871"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR8467982.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITIPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5034..5756
                     /locus_tag="ACHX5T_002872"
     CDS             5034..5756
                     /locus_tag="ACHX5T_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR8467983.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="ACHX5T_002873"
     CDS             complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="ACHX5T_002873"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR8467984.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6285..7016
                     /gene="dnaQ"
                     /locus_tag="ACHX5T_002874"
     CDS             6285..7016
                     /gene="dnaQ"
                     /locus_tag="ACHX5T_002874"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR8467985.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7149..7225
                     /locus_tag="ACHX5T_002875"
     tRNA            7149..7225
                     /locus_tag="ACHX5T_002875"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7183..7185,aa:Asp,seq:gtc)
     gene            complement(7356..8402)
                     /locus_tag="ACHX5T_002876"
     CDS             complement(7356..8402)
                     /locus_tag="ACHX5T_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFR8467986.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="ACHX5T_002877"
                     /pseudo
     CDS             complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="ACHX5T_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="ACHX5T_002878"
     CDS             complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="ACHX5T_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR8467987.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="ACHX5T_002879"
     CDS             complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="ACHX5T_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR8467988.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11362..12192)
                     /locus_tag="ACHX5T_002880"
                     /pseudo
     CDS             complement(11362..12192)
                     /locus_tag="ACHX5T_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
     gene            complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="ACHX5T_002881"
                     /pseudo
     CDS             complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="ACHX5T_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_137593242.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3008 a         3393 c         3483 g         3214 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcac caatcgtcaa actttggcct ggcttcagct tagatccgcg
     3061 cagtttgttc cactgctgca aatctttggt gcttacgccg agacgtgaag cgatgcttga
     3121 aagcgtgtcg ccagagcgta cggtgtaaac acggctgtta agcggcgtat tgttggcaac
     3181 cagcgtcgac tgtacagcag caatttcgcc tgaagcgaga gattcacgca gttgatctgc
     3241 atgcttcttt ggcaccatca cgtactgcgg cccacttgcg cccagcgtgg agcctttcac
     3301 gccagcgttg aatgtcttca gcttgctgac ggaaatcccc gccatatctg caaccttcgc
     3361 catttcaacc gggctactca ggtgcacacg cgccagagca cggctttcat cggtcgttgg
     3421 cagacgtacg ccataacgct tgctgttttt gagaatatca ctcaatgcca gcattttagg
     3481 cacgtactgc ttcgtttcct gcggcaacgg taacgaccag aagtccgtgg atttcccacg
     3541 cgctttgttc gttttaattg ccttcatgac ccgaccttcg ccgctgttat aagccgctac
     3601 ggtcagaagc cagtcgtcgt caaacatttt gttcagacgc tgcatcatat tcagcgcggc
     3661 agttgttgaa gcaacaacat cgcgacgcgc gtcataattg cgggtctgtt tcaaaccata
     3721 attgcgcccc gtgctcggaa tgatctgcca gatgcctgcg gcattggcgc cagacgttgc
     3781 gtgaggatca aaagcgctct ccactatggg tagtagtacc agttccatag gcatgttacg
     3841 ttttttaact tgccctgcta tccagtacat atacggctct gcccgtaaag ttacatcgtg
     3901 gagatagctc ttattgcgta aatatttctg tttctgttcg cgaatccggt cattttccgg
     3961 aattcccatc tttagctcgt cgccaatgaa agcccacaag tcaccatctg gcgcgataga
     4021 cgtcccatcg tccatccatc gtgcctgact tgtaaacttt gctgcttccc cttgaccagc
     4081 tgcagaaagg ctctgtgcgt gctgttgaac gttgccggta ctctggcaac ccacgagcag
     4141 gacagaggcg agtaatatcg cttttgcctt catgtgtgtg tcaatagttg cttaaaagac
     4201 gaccgatcat aacggcgaac ggagccgatg acaagaaagt tttatcagaa cctatctttc
     4261 tttgacctta accatgcaaa acgctcttca ggttgttgca ataatgtttc ttcattaatt
     4321 ccattaatta aatcaatatc ttccgttctt aaaaaaacat taatttgccg ctcatttttc
     4381 agaattacgg gtagtgttat ttgatttttt gcccgtaact ccttaacttt acgataataa
     4441 tcatttatgg acaaatcgtg cggaaaaata ctcaaagcaa acttcatatt tgataacgta
     4501 tattcatgag cacaacatac caatgtatcg tcaggtaacg cacttagctt tttaagtgat
     4561 tgatacattt gtaaagctgt cccttcaaac aaccgaccac acccaccaga aaacagcgtg
     4621 tcgccgcaaa atagataagg tttactgaag taacagatat gtcctaaagt gtgacccggt
     4681 atggtaatta cactaaattc atgccctaaa acgaaggcag tttcgccatc tttgactacc
     4741 tgtgttgttc ccttatcttg tgtctcttgt ggaccataca ctacaatttg cggaaacttt
     4801 tccaccagtt cttttacgcc gccaacgtga tcgtggtgat ggtgggttag aaatatggcc
     4861 tccggttgcc agttattggc ggtaatggcg tttaatactg gctctgcgtc tccgggatcg
     4921 acaatcaggc agcgacctgc ctcatcattc aaaacccaga tgtaattgtc atcaaaggcg
     4981 ggaatactgt taagattcat agattacctc tcagtgtaaa acggaaggtt gtgatgaaac
     5041 cggcaagagt ccctcaaact gtcgtggctc ctgattgctg gggcgatttg ccctggggag
     5101 agctttatcg caaggcgctg gagcgccagc tcaacccgtg gttcactaaa atgtatggtt
     5161 ttcatctgct taagattggc aatttaagcg cagaaatcaa ttgcgaagcg tgcgcggttt
     5221 ctcatcaagt gaatgtttct gcgcaaggaa tgcccgtcca ggtgcaggcg gatccacttc
     5281 atcttccttt tgccgataaa tccgttgata tttgtctact ggcacatacc ttgccgtggt
     5341 gcaccgatcc gcatcgttta ttacgtgaag ccgatcgggt actgattgat gatggctggc
     5401 tggtgattag tggcttcaac cccatcagtt taatgggatt acgcaaactt gtgccggtat
     5461 tgcgcaaaac ctcgccctat aatagccgga tgtttactct gatgcggcaa ctggactggc
     5521 tctctttgtt gaattttgaa gtgctgcacg ccagccgttt ccacgttctc ccgtggaaca
     5581 aacacggagg aaaactattg aatgcgcata ttcctgcgct tggttgctta caacttattg
     5641 ttgcccggaa acggactatt cctttaacgc taaatccgat gaaacagagt aaaaacaagc
     5701 cacgaattcg ccaggcggtt ggagccaccc ggcaatgtcg taaaccacag gcttaaactt
     5761 cgacttggta gcccgtatct tccagtgtgg gattcatcgc cgcggcacga gccagttcat
     5821 cacagcgttc gttttccggg tgtccggcat ggcctttaac ccattcccat ttgatttgat
     5881 gctgccccaa tgcagcatca agacgttgcc agagatcgac attttttact ggttttttgt
     5941 ctgctgtttt ccagccacgt tttttccagt tatggatcca ctgggtgata ccctggcgga
     6001 catactggct gtcggtactc aaaatgactt cgcaatgttc ttttaacgcc tccagcgcga
     6061 caatagcggc catcaactcc atacggttgt tggtggtgcg ggtgtagcct gcgctaaagg
     6121 ttttctcgcg tccgcgatag cgtaaaatag cgccgtaacc cccaggtcca ggattaccca
     6181 gacacgaacc atcggtgaaa atttctacct gtttaagcat ctctggtaga cttcctgtaa
     6241 ttgaatcgaa ctgtaaaacg acaagtctga cataaatgac cgctatgagc actgcaatta
     6301 cacgccagat cgttctcgat accgaaacca ccggtatgaa ccagattggt gcacactatg
     6361 aaggccacaa gatcattgag attggtgctg ttgaagtagt gaaccgtcgc ctgacgggca
     6421 ataacttcca tgtttatctc aaacccgatc ggctggtgga tccggaagcc tttggcgtac
     6481 atggtattgc tgatgaattt ttgctcgata agcccacgtt tgccgaagta gccgatgagt
     6541 ttatggacta tatccgcggc gcggagttgg tgatccataa cgcagcgttc gatataggct
     6601 ttatggacta cgagttttcg ttgcttaagc gcgatattcc gaagaccaat accttctgta
     6661 aggtcaccga tagccttgcg gtggcgagga aaatgtttcc cggtaaacgc aacagcctcg
     6721 atgcgctatg tgcccgctac gaaatagata acagtaaacg aacgctgcac ggggcattac
     6781 tcgatgccca gatccttgcg gaagtttatc tggcgatgac cggtggtcaa acgtcgatgg
     6841 cttttgcgat ggaaggagag acacagcagc aacaaggtga agcaacaatt cagcgcattg
     6901 tacgtcaggc aagtaagtta cgcgttgttt ttgcgacaga tgaagagctg gcagcacatg
     6961 aagcccgtct cgatctggtg cagaaaaaag gcggaagctg cctctggcgg gcataaatac
     7021 atgtaaaagg cgctaaaaat agcgacttgg gcgatttttg cagcaaacga ttcaaaagat
     7081 gagaaaaatc gttgacgaag gtcgaggcaa tccgtaatat tcgcctcgtt cccaacggaa
     7141 cacaacgcgg agcggtagtt cagtcggtta gaatacctgc ctgtcacgca gggggtcgcg
     7201 ggttcgagtc ccgtccgttc cgccaatatt cagaaagccc gaatcagtaa tgattcgggc
     7261 tttcgtcgtt ttggtgaagt gagaattttt cactccgaag taaaacggat atgatttaaa
     7321 aaatggccag acattataaa ttctggccac ttctgttact ccagcgcatc ccatttctcc
     7381 agcagttcta ctggaatttc atttattacc tccgagacgc gttttccgac caggcgttct
     7441 gcctggcgca gcaaaggaat ggtcgggctg ctcggttcgc tgtgctcaaa ccagcgacgg
     7501 atgacacgca ggcgttccag cgcgtcgttt cggtcgcgga tctgccgggg ttctgcgccg
     7561 gtcatcggtg atatttcctc cgcgatatgc accggtagcg caatctgctc tgcctgcatc
     7621 tgttcagtat gaacgatggt ggcgttttcc ggctgctcag cctgtggttg tggcaggatc
     7681 tccggcgtgg tgctctgcac tgctcccggt aacagggcca gcagttgcgt cagacgggag
     7741 aagtccggtg cctggtcatt cagcgtttca cgcgcatggc gttgcagccg ctcagtggtc
     7801 tctgctgcct ggcgaaaggc atccagcggc agtgtgccgc gcgcttccag gtctgccagt
     7861 tgctggcgta ccgattctgg tgccagcgca tccgccgggc gtaatgcaga tagcgaacgc
     7921 tccacatccc gcacctgaag gcgcatggca gcattatttg acagtgtaat gccacgaata
     7981 tccgccatta caccttcgtg gtccagcaac gccgccagcg cgttactgcg tgccaccgca
     8041 gcatcttcgg cggtgatatc ttcagtcgcg agcagttgag gatggatggc gtccgggtac
     8101 gtggagcaca acgtttccag cagcgtgagc gcctccgaca gtcccctggc accggcctgc
     8161 tggatacggc agcgcagcag gacaaccaga atgcggatat ctttgctgcg catcagcaga
     8221 cgatgggcgt cccgctcaag ctccgcccag ttaatgtttt ccggcgtact gacaaaatcg
     8281 ccatattgcg cttcttcacg cggtgcggcg cgggtgaaca gcagcaggta ttccggatcg
     8341 tactccagat ccgggccgca ggggcattcc gccgaaattg ggtcagacag gaatatttcc
     8401 atttaattat cctcagtgcg ccagcgtggt ggccagaccg tccggattgc tggactcacg
     8461 caccagatgg gccgatgccg ccagacgagc gtgatctggc aacgggctgt cagcgctctc
     8521 ccgcaaatcc tgcccgctga ggctggcgac ataaaaggca aagcggtcat gttgcggttt
     8581 atccagaccg cccccagcgg actgggcgct gcgccatgcc tggtaaaact gcgtcagcca
     8641 gcggtgatga aaaagatcag aaaaacgggt cagcgtcggc tttcagtccg gttacgcgcc
     8701 agttcggtgt agtgcagcgg cagtggaccg ttcggccccc acagcacgag gctgtacaga
     8761 ctgatgtgca ggcgcccttc ctgccagccc acactggcga tttctcgtgg cgcaaaggtc
     8821 agcgcgggcg tttgtccgag gcgaaatttt tccatccgtg gttgccaggt gttgcccgcc
     8881 ggaacggtac acaactgcac atccacgcgg cgcatcaggt tcagaaaacc gtaacgccag
     8941 gggggggcct gcgcctgagt gaaatcgttg ctcatacgct tccccgctgt cccgccctga
     9001 caggccaggt cataattttg ccgcgctgca tgctgtgcag cgtcatctgc gagaaggtat
     9061 taatggaaac atggcgggcg atatagtgtt ccagcaccag gccgaacaga tacggactga
     9121 tgccggaaaa accttcttca tccaccgtca gttcacaact gacgccacgc ccatagacca
     9181 gtagtccgga accgggcaga cggcgggtga ccggggtcgt tttacaacca atcaggctgc
     9241 gcacctggcg cgattgcgga ctgtcatgag caggaataaa caggttcagc agatctcgca
     9301 acgcctggcc gccggtacgg tgatccagat cggccagcgg cagataatta aaagataact
     9361 ggcggatcaa ccgccatgcc atctcacgtt cagccatcgg gggctgcggc gagcgtggcg
     9421 ggcggataag ccctacgccc gccaccggga tggctgcatc cacggttaga tcgtcccggc
     9481 cattgcgggc aataagacag ggcagatcgc ggttagtgac catggccgtg atggtgatgt
     9541 ggcgcagatt ttccgggtac ggcgcttcat actggtcaac cagcgacagg aacacttccg
     9601 agcccgtata gggggtacgg gtgccgtaac ggcgggcgtt ttctgataag cggcggggct
     9661 cccggcgcag ggaaaaataa cgtccgtgat tgccttcatc attattgcgg gtgtggtaga
     9721 gcgggcgaaa ggccatcttc cgtgtggtgt cggtttccag cccttccact tcctggaccg
     9781 agaacacctc ataatccagc ggatgggttc tgtccaccac cagatgctgt tccgtcgtgc
     9841 tgtgcgtgac atcgatccgg gcggtgacgc ggggaaacag gttgataacc ggggtacaga
     9901 acaggctgaa ctgtgccgca tttgtctggt ggatcagcca gtccggcggc aggcggttca
     9961 gcaggataac aatctccgcc acgccaccgt caattttctg taatccggca gacaggcctg
    10021 tcggggtgaa aaaatagaag cgttccggac aggcaaaata ttcatgcagc aggttatgac
    10081 cgtgaaacac gttccacgcc agcggcagca gcccctgatc cggctccagg ccttcgtaca
    10141 taacgggctg ttgcagattc acatccagcg cgccgtcaaa atgccccgga atgccagcca
    10201 ggggcgcgat ggcgctggta tgtagaagtt ctaacaggtg tgaggccgta cgctcctcgc
    10261 cgcagagata aaacggcagc ctgtccagtc cggcaagctg actgaacgtt agttcaccaa
    10321 aggtgcgcag ggtaatacgc agcgccccgg cgacgtgaat gttggccggc aggtagcggt
    10381 gcagggcagg catatccggt ggcgcggcgg tcagtcgcgc ctcttcgatg gcgagcggcc
    10441 acagcgttac atcctggctg ctgcgaaact ggcaggcagt gctttcgcct tccggaatgg
    10501 cggagaaaaa ggctgtgtcg cgcgggacgg taaatccttt cgccagatcg ccttcttccg
    10561 tatcgggatg caactgcgcc acgctcattg agggcgtggg tgtgacgtaa ttggggctga
    10621 ccacttccag caggcgctgg gtgaaacggg ggaattcagc gtcaattttt agctgcgtgc
    10681 gggcggtcag gaagctgaag gcctcgacca ttcgctctac atacggatcg gcaatatccg
    10741 ttccctgcat gcccagccgg gcggcgactt tgggatgacg ggcggcaaat tcggctcccg
    10801 tttcccgcag atagctcagt tcgcggttgt agtattccag tagccgtgga tccatcggtc
    10861 tctctctgaa ttaaaaaaac gtaatgcgac tctgctccat atccagcgcg ctacgtaccc
    10921 ggaatgcagt cggatacggc tgcgtgagga tctcgccgcg aatttcaaac tgtaatgtgt
    10981 tatggccact ctggcggcgc tcatcctgaa gtgggatgat ttgcagagtg gacgctttca
    11041 gccggggttc aaaccgggtg atggttcgca aaatggccct gcggatatcg tcccacttgt
    11101 gctcatacag aaagctgccc gccagtggcg gcaggccgta gttaagtacg gacgctgccg
    11161 cgtacggata acgtgcagca tcaatatcat cttcatggct gacggtgttg agcagaaatg
    11221 acaaatcccg gcggatgatc tccttaatct gaaccggact gacgctgata ccgtggtcgc
    11281 gtttctggtt tggggcgtcg tcacacaggc ggtcaaacag cgtgggcagc aggtgattgc
    11341 ctggacgctg gcgggaagcg ctcacgcgtc gcttccttca gcggtgttaa aggtacaggt
    11401 ggacaaatcc agcaggctga tatcgccgtg accggtgagc cacaccctct gacccagcgc
    11461 ctgtaccagt gtctctcccg gaccgtcctg ccagaccgtt tcccggcaca gacgcaggga
    11521 gtcggcagcc ttttcagagc cgctgtagcg gggaaacagc taggcactgt gtgtatcgcc
    11581 attgttcagg gtgacgttga cgggtttcca gatcaaatcg atcagatgcg caggccgcgg
    11641 cgatttcagc gaacagattt gtgaaaacgg cagccagata tatgctccgc ccgcgatcag
    11701 ttcgagtacc ggaccggtac gggagtcgct gtcgctaatc cagtcaaatg cgccgccatt
    11761 ccactgcccg gaagtgtctg ttatggcctc aagcgcgcgg ctgcggtgag catcagcttc
    11821 ctgcgcttca ccacgggcgt tacaggcgag ggcggtcagc agatcctcca tccaggcggg
    11881 cggcgggaga ataacaccgg gccgctgttc gccctgaaag caggcgtggc ggtatatctc
    11941 acagcggatc agctcgccaa atacctgcgc ttcgcgagta taatttgcgt ccatccgggc
    12001 gcatagctgg atctgttgca gtgcccgcga ccagtcgcct gccacgcaca ataactgaaa
    12061 caggctgtgg cggcgggagg cctgcgctgg gtgcatcctg acctgttgct ctgccatctg
    12121 aatgccctcc accacggaat aatcccgcag cagcgccgac aaggtaatcg gtagctcgtt
    12181 attgtttgtc atgatatatc tctccatgtt gtgccgggtt tatccggtaa tggctgtcaa
    12241 tgccaatgat gcgatgctct ttccgtgtca gatctggcag tacggtgtta tgcgctgttt
    12301 ccggggcgta ttccggggaa agtaaatgca ggatatcagg gggagtcttt tcgacaggcc
    12361 agatagtttc atcttcgcca gtggcatcca gtacattgag aatagcgtca atgcttaacc
    12421 caccagtgac catatcctgc aatgtctcgg cttcggtgac caggtcacgc aatgaatcca
    12481 gatcctgcga ggagggctct tcgttaaaag gaaaaggatc agcatggcga ttaagatctt
    12541 gttcgggaga taacaacgcc tcgcggtatt cctgataaag tcgggtcagg gcatttcctt
    12601 tttccgcacc ttctccggtg gaggaggctc catgaacagg aatgatatcg aatggatttt
    12661 gtgatgtcag atgctgctgc ctgaaccaat ccagatccag atattcggtt attgcttccg
    12721 acgtgatggc agtctgcgat atcgttttta ctgtgctcag gttcggagat gatggattgc
    12781 catgtacaag gcactatgaa gataaccccc attctatcag gtcgccttca ttcagacgca
    12841 tacggtgatg aggttccaca atctggtcgt ttaccgtaca gcaatattcg gtagactgat
    12901 tgataatcca ccatgcttct tcgtggcggg tcagagtgag ggcaatcttt acatcatcat
    12961 tcaccttaaa aacaggaatc cagggagagg taacggtgaa gataatttgt gttccttcag
    13021 tcgcgtaaat tgtgttatcg gcgatcccgt gcgcctgtat tttttggagt ccccacgtaa
    13081 tatttggttt cattgtgt
//
LOCUS       DAZSIO010000112        13015 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZSIO010000112 DAZSIO010000000
VERSION     DAZSIO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.112"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..319)
                     /locus_tag="ACHX5T_002882"
     CDS             complement(<1..319)
                     /locus_tag="ACHX5T_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFR8467989.1"
                     /translation="MAISDTKLRTIYGKPYSGPQELADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFD"
     gene            complement(525..1244)
                     /locus_tag="ACHX5T_002883"
                     /pseudo
     CDS             complement(525..1244)
                     /locus_tag="ACHX5T_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1260..1592)
                     /gene="cusF"
                     /locus_tag="ACHX5T_002884"
     CDS             complement(1260..1592)
                     /gene="cusF"
                     /locus_tag="ACHX5T_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR8467990.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1616..2998)
                     /gene="cusC"
                     /locus_tag="ACHX5T_002885"
     CDS             complement(1616..2998)
                     /gene="cusC"
                     /locus_tag="ACHX5T_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR8467991.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            3155..3838
                     /gene="cusR"
                     /locus_tag="ACHX5T_002886"
     CDS             3155..3838
                     /gene="cusR"
                     /locus_tag="ACHX5T_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR8467992.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3828..5270
                     /gene="cusS"
                     /locus_tag="ACHX5T_002887"
     CDS             3828..5270
                     /gene="cusS"
                     /locus_tag="ACHX5T_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR8467993.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5420..7657
                     /gene="nrfB"
                     /locus_tag="ACHX5T_002888"
     CDS             5420..7657
                     /gene="nrfB"
                     /locus_tag="ACHX5T_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFR8467994.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7644..10612
                     /gene="nfrA"
                     /locus_tag="ACHX5T_002889"
                     /pseudo
     CDS             7644..10612
                     /gene="nfrA"
                     /locus_tag="ACHX5T_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10613..11503
                     /locus_tag="ACHX5T_002890"
     CDS             10613..11503
                     /locus_tag="ACHX5T_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR8467995.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11686..12447
                     /gene="envY"
                     /locus_tag="ACHX5T_002891"
     CDS             11686..12447
                     /gene="envY"
                     /locus_tag="ACHX5T_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR8467996.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12493..12569)
                     /locus_tag="ACHX5T_002892"
     tRNA            complement(12493..12569)
                     /locus_tag="ACHX5T_002892"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12533..12535),aa:Arg,seq:tct)
     gene            12812..>13015
                     /locus_tag="ACHX5T_002893"
     CDS             12812..>13015
                     /locus_tag="ACHX5T_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFR8467997.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3186 a         3376 c         3304 g         3149 t
ORIGIN      
        1 tgtcgaacca gtagtcgaga cactgggcga ccgtcatgga gttctccacc ttctcttcaa
       61 aataagtgcg tggatccgtt cctgagaaat agagctttcg caagtcagca gtgatctgtc
      121 tggcatcctt caaagacagg gatgggtatc gaccaagccc aagtcgatta ggcttgccat
      181 gccagcgata gcggtactgg aactggatga cccccttcgg tgaaattcgt acgctgaggc
      241 catcagcatc agccaattct tgtgggccag aatatggttt accataaata gtacgcagtt
      301 ttgtgtcgct tatagccata aataatattt ggtacgctca gaaataatgg tttggtacac
      361 atctggtaca caatatcaca tggacaacag cgcacagtaa acaactatat ttgacgttcg
      421 tagacatact caggtgaaaa aaaggttgtt tttcgagtta taacagacag ttaattaact
      481 acagtggaca atgctagaca atgacagaca cagataagca acctaccttc ctctttcgcg
      541 accacgttat ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca
      601 tcaatgggcg ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggtggcga
      661 atatccgcct ccggcattcc cgccagtcgc agccgctcaa gaatgccttc agtctgggtc
      721 gccgtaccgc cggtttcgcg caacagtaaa tactcactct gcgcttccac ccagtcggga
      781 atggtcaggt cgagaagcgg tgtgcccttt tgtactttat cgcccacggt aagcggatac
      841 accttgtcga taaacccggc agcgcgagcc tgcacaatgg catactgata ctcgttgtaa
      901 ctgacattcg tcgggaaact ctgggcaaaa gtcagcggcc cgcgcgtgac ggtagccgtt
      961 ttcaccccca gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct
     1021 tcatcggcat atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg
     1081 aaccgcgtat tgggatacat cgggtcgtac cagaataaga ttttacgttc tgcggtcggc
     1141 attttttctg cgtgcggttc cgcctggtca aaccaggtaa aacctgccgc tgaaataata
     1201 ccgcccgcga tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt
     1261 tactggctga ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac
     1321 gccactttgt cgccggtttt aatttcactc attttcgtct gcggggtgat ggtaaagcgc
     1381 atggtcatct ccggccagtt gacggcagca atcggatcgt gatggatggt gatttttttg
     1441 ctttccagat caacaccttt taccacaccg gtggcactaa tgacctgtgg ttgtgcttcg
     1501 ctcatggttt catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga
     1561 ctgaacattg cgacttgcag tgcttttttc atttattctc tcctggagtt aaaaattatt
     1621 gctgccaacc gccccctaag gcggtataca aataaatttc gttaacctga cgggcataat
     1681 tcaggtcaag taaagtttgt cgggttgcaa ataaagaacg ctcggcatcc agcacttcca
     1741 gataacttac cgcgccgtgc tgatataatg cccgcgcccg ttgcagggta atttgcagcg
     1801 acgccagata acgctgctgg gcgctgattt gatcgtccag gctttgacgt aatgccagcg
     1861 catctgccac ctctttaaag gcgttctgga ttttctgttc ataattcacc accgactgct
     1921 gctggcgaat ttcggcgata tccagattgg cctggttgcg tccggcatta aaaatgggga
     1981 tctcaatttt aggaataaaa ttccacatcc cgctgctagc gttaaataat gacgacagat
     2041 cgctgctggc ggttgatatt ccgctggtca ggctgataga cgggaaaaat gccgcacgcg
     2101 cagcaccaat attagcatta gccgccatta acgcgtgttc agcttccata atatccgggc
     2161 gctgcaataa gatttgcgac gacaagcccg ccggtaattt aacgctttgc aggctgccgc
     2221 tgtttactgt ctgcgcttgc ggtagcttgc cgtagcttcc caataacagt tgcaatgcat
     2281 tattcgcctg cgccagttcc ccctgacgtt tagcgatgtc actgcgggta ctttctatca
     2341 ccccgcgagc ctgttccagc gccagaacat tgctgctacc ggtcaacagt tgtttttcga
     2401 caaacgcata tgactgctga taattacgca gcgtttcttc ggctatttgc aattgcgcat
     2461 acgccagctg ctgattgaaa tagctttgcg cgacattaga aaccagcaaa atatgcaccg
     2521 cgcgctgggc ttcctcagtg gctaaataat tttggcgctc ggcttcgctc atgttcttta
     2581 agcgaccgaa aaaatcgaga tcaaagctgg cgttaaggcc agtcgagaac tcccgcgtcg
     2641 tggctgaatc gcctttaaga ttgccgctcc agctgccgct gccctcgcca ttgagctgtg
     2701 ggtagcggtc ggcatcggtc agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg
     2761 ccatgcgcaa atcccggtta ttcaccagcg cctcgctaat cagcgtcttc acctgattat
     2821 caacaaaaaa ggtgcgccag cccacgttct ggtagttatc cgctgcgtta accaggccgt
     2881 tctggctgag tgagaactgc tgcggtacgg gcattaccgg acgctgataa tccggtgcca
     2941 gtgaacaacc agttagcgca agggccacac aaaatggcag aagtttacaa ggagacatag
     3001 gctcataatt tctggtgatt ttatgtcgcc aactttactc gccaggctct gattttccgg
     3061 tgacaggaaa atgacaaaat tgtcattttg ccaataagcg attgccatct gatcccgcta
     3121 ctctagaatt gcccgggcaa catgcggagg aaatatgaaa ctgttgattg tcgaagatga
     3181 aaagaaaacc ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt
     3241 ggccgacaac gggctgaatg gctaccatct ggcgatgacc ggtgattatg acctgataat
     3301 cctcgatatt atgctgccag acgtgaacgg ctgggatatc gtgcgcatgt tgcgctccgc
     3361 caataaaggg atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa
     3421 ggggctggag ttgggggcag atgactacct ggtgaaaccg ttcgcttttg ctgaactgct
     3481 ggcgcgggtg cgcactctcc tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca
     3541 ggttgccgat ctgatggtcg atctcgtcag ccgcaaagtc acccgcagcg gcacgcgcat
     3601 cactctgacc agtaaagaat ttactctgct ggagtttttt ctccgccatc agggcgaagt
     3661 gctgccccgc tcgcttatcg cctcgcaggt atgggacatg aatttcgaca gcgacactaa
     3721 cgccattgat gtggcggtga agctgctacg cggcaaaatc gacaacgact ttgagccgaa
     3781 gctgattcaa accgtgcgcg gcgtgggtta catgcttgag gtgccggatg gtcagtaagc
     3841 catttcagcg cccgttttcg ctggcaaccc gcctgacctt ttttatcagc caggccacca
     3901 tcgcggcgtt tttcgccttt gcatggatca taatccactc agtaaaagtg cattttgccg
     3961 agcaggacat taatgattta aaagagatta gcgccaccct tgaacgggta ctaaatcacc
     4021 ctgacgaaac gcaagcccga cgcttaatga cgctggaaga tatcgtcagt ggttattcca
     4081 acgtgttgat ttccctggca gatagccacg gcaaaacggt gtatcactcc cccggtgcgc
     4141 ctgatatccg cgagtttgca cgtgatgcca tacccgataa agacgcgcgg ggtggcgagg
     4201 tgtatctcct ttccggcccg acgattatga tgccaggcca cggtcacggg catatggaac
     4261 acagcaactg gcggatgatt aacttgccgg ttggcccgtt ggtggacggc aaaccgattt
     4321 atacgctcta catcgcgctg tcgatcgatt ttcatcttca ttacataaat gatttgatga
     4381 ataaacttat tatgaccgca tcggtaatca gcatcctgat cgtctttatc gtgctgttgg
     4441 cggtacataa aggtcacgcg ccgatccgca gcgtcagccg tcaaatccag aatatcacct
     4501 cgaaagatct cgacgttcgc cttgacccgc agaccgtgcc gattgagctg gaacagctgg
     4561 tactgtcgtt caaccatatg atcgagcgta ttgaggatgt ctttactcgc cagtccaatt
     4621 tctcagcgga tatcgcccac gaaattcgca caccaattac taatctcatt acgcaaacgg
     4681 aaatcgccct cagccagtcg cgcagccaga aggagctgga agatgtgctc tactctaatc
     4741 tcgaagagct gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc gctcaggccg
     4801 ataacaacca gctaatcccc gaaaagaaaa tgctcaacct ggcggatgaa gtcggcaaag
     4861 tgttcgattt tttcgaggcg ctagcggaag atcgcggcgt ggagttgcgg tttgttggcg
     4921 acaaacgtca ggttgcgggc gatccgttga tgctgcgtcg ggcgctaagc aacctgcttt
     4981 ctaatgccct tcgttatacg ccaaccagag agacaattgt agtacgctgt cagacggtcg
     5041 atcaccaggt gcaagtttcc gtcgaaaacc ccggtacgcc cattgcgccc gagcacttac
     5101 cgcgattgtt tgaccgtttc tatcgcgttg acccctcccg ccagcgaaaa ggtgaaggta
     5161 gcggtattgg gctggcgata gtgaaatcga ttgttgtcgc gcataaaggc acggttgcgg
     5221 taacgtcaga tgcgcggggg acaaggtttg ttatcacatt acccgcttaa ttattcagca
     5281 aacggcaact tttataacca gtgtaaaaat aacgtgccgc aatagtcata catattaatg
     5341 gtaaaaagct gtcacaattc ataaaaaacc ttaatatacg ccaccctaaa cataaccagc
     5401 gttaatgtaa ggtttttgtg tggactggct tcttgatgtt tttgctacct ggctctacgg
     5461 cttaaaagta atcgcgataa cgttagcggt catcatgttc atcagcgggc tggacgattt
     5521 ttttattgat gtcgtctact gggtacgccg cattaaacgc aagttgagtg tttatcgccg
     5581 ctacccgcga atgagttacc gcgaactgta taaaccagat gaaaaaccgt tagcgattat
     5641 ggttccggcg tggaatgaaa cgggcgtcat cggcaatatg gccgagctgg cggcgaccac
     5701 gctcgactac gaaaactatc atatctttgt tggcacctac cccaacgacc ccgatactca
     5761 acgtgatgtt gacgaagtgt gcgctcgctt tccgaacgtg cataaggtag tctgcgcgcg
     5821 tcctggtccc accagcaaag ccgactgcct gaacaacgtg ctggacgcca tcacccagtt
     5881 tgaacgtagc gccaatttcg cttttgctgg ttttattctg catgacgccg aagatgtgat
     5941 ttcaccgatg gaattgcgtc tgttcaacta tctggtcgag cgtaaagatc tgattcagat
     6001 cccagtgtat ccgtttgaac gcgaatggac gcacttcacc agcatgactt acattgatga
     6061 gttttcagaa ctgcatggca aagatgttcc ggtgcgtgaa gccctcgccg gacaagtgcc
     6121 cagcgcaggc gtcggcacct gtttcagccg ccgcgccgtg accgcactgt tagctgacgg
     6181 taacggtatt gctttcgacg tgcagagtct tactgaagat tacgacattg gcttccgcct
     6241 gaaagaaaaa ggtatgacgg aaatttttgt ccgttttccg gtggtggacg aagccaaaga
     6301 acgcgagcag cgtaaatttt tacagcacgc acggacgtca aacatgatct gcgtgcgcga
     6361 atatttcccc gatacctttt cgactgcggt tcgacaaaaa tctcgctgga tcatcggcat
     6421 tgttttccag ggctttaaaa cccataaatg gacctccagc ctgacgctga actactttct
     6481 ctggcgcgac cgcaaagggg caatcagtaa ctttgtcagc ttcctcgcga tgctggtgat
     6541 gatccagctt ttgctgttgc tggcgtatga aagtttgtgg cccgatgcct ggcatttcct
     6601 ttctattttt agcggcagcg catggttaat gaccctgctg tggctaaact ttggcttgat
     6661 ggttaaccgc atcgtgcagc gagtgatttt cgtcactggc tactacggcc tcacgcaggg
     6721 cctgctttcc gtcctgcgtc ttttctgggg caacctgatt aactttatgg ctaactggcg
     6781 cgtgctgaaa caggttcttc agcacggcga tccgcgtcgc gtcgcgtggg ataaaacaac
     6841 gcatgacttc cccagcgtca ctggcgatac ccgctcgttg cgcccgttag gtcaaattct
     6901 gctggaaaat caggtcatca ctgaagaaca actcgataca gcactgcgta atcgcgtcga
     6961 aggtctacgc ctgggcggtt caatgctgat gcaggggctg attagcgccg agcagctggc
     7021 acaggcgctg gcagagcaaa acggcgtggc gtgggaatcc atcgatgcct ggcagatccc
     7081 gtcctcgctg attgccgaaa tgccagcctc cgtagcgctg cattatgcag tactgccgct
     7141 gcgtctggaa aatgatgagt taattgtcgg cagtgaagat ggtattgacc cggtttcgct
     7201 ggcggccctg acgcgtaaag tcggacgcaa agtgcgttac gtcattgttc tgcggggaca
     7261 aattgtcaca gggttacgcc actggtatgc acgccgacgc ggtcacgatc cgcgggcaat
     7321 gttgtacaat gcggttcagc atcagtggct cacggaacag cagaccggtg aaatctggcg
     7381 gcaatatgtg ccgcatcagt tcctgttcgc cgaaatactg accacgctcg gtcatattaa
     7441 tcgttcagca attaacgtgt tgttattgcg ccatgaacgc agttctctgc cgctcggcaa
     7501 gtttttggtc accgaaggcg ttatcagcca ggaaacgttg gatcgcgtcc tgacaattca
     7561 acgcgaatta caagtttcga tgcaatcact attactcaaa gcaggtttaa acacagaaca
     7621 ggttgcgcaa ctggagtccg aaaatgaagg agaataacct taatcgcgtc atcggatggt
     7681 ctggtttact gctgacgtct ttattgagta ccagcgcact cgcagacaat atcggcacca
     7741 gcgcagaaga gctggggctg agcgattatc gccattttgt tatttatccc cgtctcgata
     7801 aggcgctgaa ggcacagaaa aataacgacg aagcaaccgc catccgcgaa tttgaatata
     7861 tacaccaaca ggtgccggat aatattccgc tgactttata ccttgcggaa gcctatcgtc
     7921 attttggtca tgatgaccgg gcgcggctgt tgcttgagga tcaactgaaa cgtcacccag
     7981 gagatgcccg acttgagcgc agtctggcgg ctattccagt tgaagtgaaa agcgttacga
     8041 ctgttgaaga actgcttgcc cagcaaaaag cgggcgacgc tgcgccgacc ctgcgttgtc
     8101 gcagtgaagt cgggcagaat gccctgcggc tggcacaatt acctgtcgcc agagcgcaac
     8161 tgaacgatgc gacgtttgct gcatcgccgg aaggaaaaac gctgcgaacc gatctgctgc
     8221 aacgggcaat ctacctgaaa caatgatccc aggcagatac gctatacaat gaagcacgcc
     8281 agcagaacac attaagcgcg gcagaacacc gtcagtggtt tgacgtgctt cttgccgggc
     8341 agctggacga tcggatcctg gcactgcaat cacaggggat cttcaccgat cctcagtcat
     8401 atattactta cgcgaccgcg ctggcttatc gtggcgaaaa agcacgcctc cagcattatc
     8461 tcattgaaaa taagccgctg tttaccacgg gcgcacaaga gaaaagttgg ctctatctgt
     8521 tatctaaata cagcgccaac cccgttcagg cattggcgaa ttatacggtg cagtttgccg
     8581 ataaccgcca gtatgttgtt ggcgcgacgc taccggtgct gttaaaagaa ggtcagtacg
     8641 acgcagcgca aaaactgctc gccaccctcc ccgccaatga aatgcttgag gagcgttatg
     8701 ctgtcagcgt ggcgacccgt aacaaggctg aatctctgcg tctggcacga ttgctgtatc
     8761 agcaagaacc gacaaatctt ccccgcctgg atcaactgac ctggcaactg atgcagaacg
     8821 agcagtcacg cgaagctgcc gatttattgc tgcaacgcta tcctttccag ggcgatgcgc
     8881 gtgtcagcca gactttaatg gcgcgactgg cgtctctgct ggaaagtcat ccttacctag
     8941 caacgccggc gaaggtggcg attttatcga aacccttacc gctggcggag caacgtcagt
     9001 ggcaaagtca gttgccgggt attgcagata attgcccggc aatagttcgc ttgctgggcg
     9061 atatgtcgcc ttcctacgat gctgccgcct ggaaccgtct ggcaaagtgt tatcgggaca
     9121 cgctacccgg tgtggcgttg tatgcatggc ttcaggccga acaacgacaa ccgagcgcct
     9181 ggcaacatcg tgcggtagcc tatcaggcgt atcaggttga ggactacgcc accgcactgg
     9241 cggcctggca gaaaatcagt cttcacgaca tgagcaatga tgatctgctc gccgccgcca
     9301 acaccgctca ggcagcggga aatggcgcag ctcgcgatcg ctggcttcaa caggcagaac
     9361 aacgtgagct gggaaacaat gctcaatact ggtggctgca tgcgcgacgt tatatcccag
     9421 ctcagccaga actcgcactg aacgatctca cgcgctcaat caatattgcc ccttctgcca
     9481 actcttacgt tgcgcgggcg acaatttatc gccaacgtca taatgttccg gccgcggtga
     9541 gtgatttgcg cgccgcgctg gaactggaac caaataatag caacacccag gcagcgcttg
     9601 gttacgcctt gtgggatagc ggtgatatcg cacagtcgcg ggaaatgctc gaacaggcgc
     9661 ataaagggct tccggacgat ccggcactga tccgacaact ggcctacgtg aaccagcgtc
     9721 tggatgacat gcctgcgacg cagcactacg cccggctcgt gattgatgac attgataatc
     9781 aggcataacc ccactgaccc cagagcaaaa tcagcaacgc ttcaatttcc gccgtttgca
     9841 tgaggaggtc ggtcgccgct ggacgttcag tttcgattct tccatcggct tgcgttccgg
     9901 cgcaatgagt accgctaaca ataatgtcgg cggtgcagcg cctgggaaaa gctatcgtag
     9961 ctacggacaa ctggaagccg agtaccgcat cggacgcaat atgctgctgg aaggcgacct
    10021 gctctcagtt tatagccgcg tctttgccga taccggagaa aacggggtga tgatgccggt
    10081 gaaaaatccg atgtccggca ccggtctgcg ctggaagccg ctgcgcgatc agatcttttt
    10141 cctcgccgtc gaacagcagt tgccgctgaa cgggcaaaat ggcgcatccg ataccatgct
    10201 gcgcgccagc gcctcattct ttaatggcgg caaatacagc gacgaatggc acccgaacgg
    10261 ttcaggctgg tttgcccaaa acctgtacct cgatgcggcg caatatatcc gccaggatat
    10321 tcaggcgtgg acggcagatt atcgcgtcag ctggcatcag aaggtagcta acggacagac
    10381 tattgagcct tacgctcacg ttcaggacaa cggctatcgt gataaaggca ctcagggcgc
    10441 gcagcttggc ggtgtcgggg tccgctggaa tatttggacc ggcgagacgc actacgacgc
    10501 ctggccgcac aaagtcagtc tcggcgtcga gtatcaacat acctttaagg cgattaatca
    10561 acgtaacgga gagcgcaaca acgcgtttct caccattgga gtgcactggt aaatgcgtaa
    10621 gttcattttc gtattgctga cactgctttt ggtcagccct ttttcctttg cgatgaaagg
    10681 tattatctgg caaccacaaa accgagatag tcaggttacc gatacccagt ggcaggggct
    10741 gatgagtcag ttacgttttc aaggcttcga taccctggtt ttgcaatgga cccgttacgg
    10801 cgatgcattt acccagccag aacagcgcac gttattgttt aagcgtgccg cagctgcgca
    10861 acagactggc ctgaagctga ttgtcgggct gaacgccgat ccggaatttt ttatgcacca
    10921 gaaacagtcg tctgcagcgc tggaaagcta tcttaatcgc ctgctggctg ccgatctcca
    10981 gcaagccaga ttatggagcg ccgcgcctgg cataacgccg gatggctggt acatcagcgc
    11041 ggaaattgac gacctgaact ggcgcagcga agccgcccgt caacctttgc taacatggtt
    11101 aaacaacgcg cagcggctga ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt
    11161 tttcgccgga aacatgtcgc ccgatggcta tcgccaactg ctggaacaag ttaaagcaac
    11221 cggagttaat gtctgggtac aggatggcag cggcgtggat aaacttaccg ctgaacagcg
    11281 tgaacgttat ctacaggcca gcgccgattg ccaaagctcc gccccagcaa gcggcattgt
    11341 ttatgaactg tttgtcgccg gcaaaggcaa aacctttaca gcgaaaccga aaccggacgc
    11401 agaaattgcc tcgctgttag cgaaacgttc ctcttgcggt aaagacatgc tctatttctc
    11461 tctgcgctat ttgcccgtcg cgcacggcat tctcgagtat taaatcccct ccaggtaagt
    11521 cgggtacgac ctggcttacc tctttcgctc tcgtcagaat tccatcaaga ttaattgcta
    11581 aaaagcatgc taaagaaaga agatcgttat cggcgaacgg gccgatcact catgcaggaa
    11641 gcatagatcc tttgagcgga gtttctgata tctgaggagt gcgaaatgca attgagcagc
    11701 agtgaacctt gcgtggtgat cctgaccgaa aaagaggtag aggtaagcgt caataaccat
    11761 gctacgttta cccttccgaa aaactacctg gccgccttcg cgtgcaacaa taacgtcatt
    11821 gaactctcaa cgttaaatca cgtattaatc acccacatca accgtaacat catcaacgat
    11881 tatctgttgt ttttaaataa gaaattaacc tgtgtaaagc cctggtcgcg gctagcaacc
    11941 ccggttatcg cttgtcatag ccgtacaccg gaagtgttcc ggctagccac caaccacagc
    12001 aagcagcaat ccagcaaacc ctgcgaagcg gagttgacgc gcgcattact ttttaccgta
    12061 ttgtctaact ttcttgagca atcgcggttt attgccctac tgatgtatat cttacgcagc
    12121 agcgtccgcg acagcgtctg ccgcattatt caaagcgata ttcagcatta ctggaatctg
    12181 cgaattgtcg ccagttcgct atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac
    12241 gaaaatacca gctatagcca gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg
    12301 ttattgatgg ataacaaaaa tatcactcag gtggcgcaat tatgtggcta tagcagcacg
    12361 tcgtacttta tctctgtttt taaggcgttt tacagcctga caccgttgaa ttatctcgcc
    12421 aaacagcgac aaaaagtgat gtggtgaagg gcaaagcgga aacggataag acgggcataa
    12481 atgaagaaga aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc
    12541 gttgctctat ccaactgagc taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga
    12601 attatacggt caacccttgc tgagtcaatg gcttttgatc tggttgctga acaagtgaac
    12661 gaccgcgtct gattttctga tttatttcgc tatagcggca aacaaacgca caccgctgcg
    12721 cgtctgaatc aagaaaaccc gtattttcat gtatcaaagt acaatttacc gccctaacgg
    12781 aaaattgtcc gctcctatga gactggtaac tatgaaacca acgtcggtga tcattatgga
    12841 tactcatcct atcatcagaa tgtctattga agttctgttg caaaaaaaca gtgaattgca
    12901 gattgtcctg aaaacggatg attaccgcat aaccatcgat tatctccgaa cccgtcctgt
    12961 tgatttaatc attatggata tagacttgcc cggaacagac ggttttacct tcctg
//
LOCUS       DAZSIO010000113        12908 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZSIO010000113 DAZSIO010000000
VERSION     DAZSIO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.113"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="ACHX5T_002894"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="ACHX5T_002894"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFR8467998.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="ACHX5T_002895"
     CDS             complement(1806..3224)
                     /locus_tag="ACHX5T_002895"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR8467999.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="ACHX5T_002896"
     CDS             complement(3378..3722)
                     /locus_tag="ACHX5T_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFR8468000.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="ACHX5T_002897"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="ACHX5T_002897"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR8468001.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5653)
                     /locus_tag="ACHX5T_002898"
                     /pseudo
     CDS             complement(4712..5653)
                     /locus_tag="ACHX5T_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="ACHX5T_002899"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="ACHX5T_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR8468002.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="ACHX5T_002900"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="ACHX5T_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR8468003.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="ACHX5T_002901"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="ACHX5T_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR8468004.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="ACHX5T_002902"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="ACHX5T_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR8468005.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="ACHX5T_002903"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="ACHX5T_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR8468006.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="ACHX5T_002904"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="ACHX5T_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR8468007.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="ACHX5T_002905"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="ACHX5T_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFR8468008.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11368..12483
                     /gene="ydeE"
                     /locus_tag="ACHX5T_002906"
                     /pseudo
     CDS             11368..12483
                     /gene="ydeE"
                     /locus_tag="ACHX5T_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="ACHX5T_002907"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="ACHX5T_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFR8468009.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            12705..12808
                     /gene="mgtT"
                     /locus_tag="ACHX5T_002908"
                     /pseudo
     CDS             12705..12808
                     /gene="mgtT"
                     /locus_tag="ACHX5T_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
BASE COUNT         3401 a         3299 c         2950 g         3258 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc caaggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagagcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgagaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    12901 ctcaatga
//
LOCUS       DAZSIO010000114        12506 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZSIO010000114 DAZSIO010000000
VERSION     DAZSIO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.114"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="ACHX5T_002909"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="ACHX5T_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR8468010.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     misc_feature    547..670
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            717..1616
                     /gene="hisG"
                     /locus_tag="ACHX5T_002910"
     CDS             717..1616
                     /gene="hisG"
                     /locus_tag="ACHX5T_002910"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR8468011.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1622..2926
                     /gene="hisD"
                     /locus_tag="ACHX5T_002911"
     CDS             1622..2926
                     /gene="hisD"
                     /locus_tag="ACHX5T_002911"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR8468012.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2923..3993
                     /gene="hisC"
                     /locus_tag="ACHX5T_002912"
     CDS             2923..3993
                     /gene="hisC"
                     /locus_tag="ACHX5T_002912"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR8468013.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3993..5060
                     /gene="hisB"
                     /locus_tag="ACHX5T_002913"
     CDS             3993..5060
                     /gene="hisB"
                     /locus_tag="ACHX5T_002913"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR8468014.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5060..5650
                     /gene="hisH"
                     /locus_tag="ACHX5T_002914"
     CDS             5060..5650
                     /gene="hisH"
                     /locus_tag="ACHX5T_002914"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR8468015.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5650..6387
                     /gene="hisA"
                     /locus_tag="ACHX5T_002915"
     CDS             5650..6387
                     /gene="hisA"
                     /locus_tag="ACHX5T_002915"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR8468016.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6369..7145
                     /gene="hisF"
                     /locus_tag="ACHX5T_002916"
     CDS             6369..7145
                     /gene="hisF"
                     /locus_tag="ACHX5T_002916"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR8468017.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7139..7750
                     /gene="hisIE"
                     /locus_tag="ACHX5T_002917"
     CDS             7139..7750
                     /gene="hisIE"
                     /locus_tag="ACHX5T_002917"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR8468018.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="ACHX5T_002918"
     CDS             complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="ACHX5T_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR8468019.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="ACHX5T_002919"
                     /pseudo
     CDS             complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="ACHX5T_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="ACHX5T_002920"
     CDS             complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="ACHX5T_002920"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR8468020.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11944..12258)
                     /locus_tag="ACHX5T_002921"
     CDS             complement(11944..12258)
                     /locus_tag="ACHX5T_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468021.1"
                     /translation="MKKASEYSELYGDNLIQLGLEDGIYFYKGMAIGDVFGLARYSDW
                     TISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYPKFKVLMDTPNGILIA
                     IK"
BASE COUNT         3184 a         3159 c         3107 g         3056 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgaa ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
      601 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
      661 gcttttttat tgcgcggttg ttaacggttc agacaggttt aaagaggaat aagaaaatga
      721 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
      781 gcgaattact ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
      841 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgatgacatt ccctgtctgg
      901 taatggacgg cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa gaagagctgc
      961 ttaaccgccg cgcccagggt gaagatccgc gctactttac cctgcgtcgt ctggatttcg
     1021 gcggctgccg cctttcgctg gcaacgccgg ttgatgaagc ctgggacggt ccgctctcct
     1081 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     1141 aaggtatctc ttttaaatcc tgcttactga acggttctgt cgaagttgcg ccgcgtgccg
     1201 ggctggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     1261 tgcgcgaagt cgaagttatc taccgctcga aagcctgcct gatccagcgc gatggcgaaa
     1321 tggaagaatc taaacagcaa ctgatcgaca agctactgac ccgtattcag ggtgtgatcc
     1381 aggcgcgcga atcaaaatac atcatgatgc acgcaccgac cgaacgcctg gatgaagtca
     1441 tcgccctgct gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt gatcaacagc
     1501 gcgtagcgat gcacatggtg agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     1561 aagcactggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     1621 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     1681 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcaccgtta acgatattct
     1741 cgataacgtg aaaacgcgtg gcgatgaggc cctgcgtgaa tacagcgcga agtttgataa
     1801 aaccacggtt accgcgctga aggtgtctgc tgatgagatc gccgccgcca gcgaacgcct
     1861 gagcgacgag ctaaaacagg cgatggcggt ggcagtaaag agtattgaaa ccttccacac
     1921 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcta ggcgtgcgtt gccagcaggt
     1981 cacgcgcccg gtggcttcag tgggtttgta tattccgggc ggctccgccc cgctcttctc
     2041 aacggtatta atgctggcaa ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     2101 ctcaccgccg ccgattgccg atgaaattct ttatgcggcg cagctgtgcg atgtgcagga
     2161 cgtgtttaac gtcggcggcg cacaggccat tgccgcgctg gcgtttggta cggaatctgt
     2221 gccgaaagtg gacaaaatct tcgggccggg taacgccttt gtcaccgaag caaaacgcca
     2281 ggtaagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgct
     2341 ggtgattgct gacagcggcg ctacgccgga ttttgtggct tctgatttgc tttctcaggc
     2401 tgaacacggc cctgactcac aggtaatttt actgaccccc gatgctgata tggcgcgtcg
     2461 cgttgccgag gccgtcgaac gccaactggc agaactgccg cgtgccgaaa ccacccgcca
     2521 ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tggagatctc
     2581 caaccagtac ggcccggagc acctgatcat tcagacccgc aacgcccgcg aactggtcga
     2641 tggcatcacc agcgccggtt cggtatttct tggtgactgg tcaccggaat cggcaggcga
     2701 ctacgcctcc ggcaccaacc acgtgctgcc gacttacggt tacaccgcca cctgttccag
     2761 cctcgggctg gcagatttcc agaaacgcat gaccgtacag gaactgtcga aagaaggttt
     2821 ctccgcgctg gcttcgacca ttgaaacact ggccgccgcc gagcgcctga ccgcccacaa
     2881 aaatgccgtt actttgcgtg ttaacgtcct taaggagcaa gcatgagcac cgtgactatt
     2941 actgatttag cgcgtgaaaa cgtccgcaac ctgacaccgt atcagtcggc gcgtcgtctg
     3001 ggcggtaacg gcgacgtctg gctgaacgcc aacgaatatc ccacagccgt ggagtttaag
     3061 cttactcagc aaacgctcaa ccgctacccg gaatgccagc caaaagcggt gattgaaaat
     3121 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     3181 ggtattgaac tgctgatccg cgctttttgc gaaccgggta aagacgccat cctctactgc
     3241 ccgccaacgt acggcatgta cagcgtcagc gctgaaacca ttggtgtcga atgccgcaca
     3301 gtgccgacgc tggaaaactg gcaactggac ttacagggca tttccgacaa gctggacggc
     3361 gtaaaagtgg tctatgtttg cagccccaac aaccccaccg ggcaactgat caatccgcag
     3421 gattttcgca ccctgctgga gctaacccgc ggtaaggcga ttgtggttgc cgatgaagct
     3481 tatatcgagt tttgcccgca ggcatcgtta gctggctggc tggcggaata tccgcacctg
     3541 gcgattttgc gtaccctttc gaaagccttc gcgttggcgg ggcttcgttg cggatttacg
     3601 ctggcaaacg aagaagtcat caacctgctg atgaaagtga tcccccccta cccgctctcg
     3661 acgccggttg ccgacattgc cgcacaggcg ttaagcccgc agggaatcgt cgccatgagc
     3721 gaacgggtgg cgcaaattat tacagaacgc gaatacctga ttgccgcact gaaagagatc
     3781 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     3841 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     3901 caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     3961 gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt tttatcgatc
     4021 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgacaaac
     4081 tcgcctttga accgggcgtg atcccggaat tgctgaagct gcaaaaagcg ggctacaagc
     4141 tggtgatgat cactaatcag gatggtctgg gaacacaaag tttcccgcaa gcggattttg
     4201 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     4261 tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac
     4321 tggtggaggg ttatctggct gagcaagcga tggatcgtgc caacagttat gtaattggcg
     4381 atcgcgcgac cgacattcaa ctggcggaaa acatgggcat taatggttta cgctacgacc
     4441 gcgaaaccct gaactggcca atgattggcg agcaactcac taaacgagac cgttacgccc
     4501 atgtagtgcg caacaccaaa gagacgcaga ttgacgtcca ggtgtggctg gatcgcgaag
     4561 gtggcagcaa gattaacacc ggcgttggct tctttgatca catgctggat cagatcgcca
     4621 cccacggcgg tttccgcatg gaaatcaatg tcaaaggcga cctctatatc gacgatcacc
     4681 acaccgtcga agataccggc ctggcgctgg gcgaagcgtt aaaaattgcc ctcggcgaca
     4741 aacgcggtat ttgccgcttt ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg
     4801 cactggatat ctctggtcgc ccgcacctgg aatataaagc cgagtttacc taccagcgtg
     4861 tgggcgatct cagcactgaa atgatcgaac acttcttccg ttcgctctct tacaccatgg
     4921 gcgtgacact acacctgaaa accaaaggta aaaacgatca tcaccgtgta gaaagcctgt
     4981 tcaaagcctt tggtcgtacc ctgcgccagg ccatccgcgt cgaaggcgac accctgccct
     5041 cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat actggctgcg ccaacctgaa
     5101 ctctgtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     5161 cattgtgttg ctggccgata aactgttttt acccggcgtc ggcaccgcac aagcagcgat
     5221 ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtgct
     5281 gggcatctgc ttagggatgc aactcctggg acgacgcagc gaagagagca atggcgtcga
     5341 cttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc tgccactgcc
     5401 acatatgggc tggaaccgcg tttatccgca ggcaggcaac cgcctgtttc aggggattga
     5461 agacggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
     5521 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
     5581 cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
     5641 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
     5701 cagggcgatt acggcaaaca acgcgattac ggtaacgacc cgctgccgcg cttacaggat
     5761 tacgctgcgc aaggtgccga agtgttgcac ctggtggatc tgaccggggc aaaagatccg
     5821 gctaagcgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt cccggtgcag
     5881 gttggcggcg gtgttcgtag tgaagaagac gtggcggcgt tactggaagc tggcgttgcg
     5941 cgcgtggtgg tcggttccac cgcagtgaaa tcaccggacg tagtgaaagg ctggtttgaa
     6001 cgtttcggtg ccgatgcgct ggtgctggca ctggatgtcc gtattgacga gcaaggcaac
     6061 aagcaagtgg cagtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
     6121 gaaacctatc tgcccgtcgg cctgaaacat gtgttgtgta ccgatatctc gcgcgacggc
     6181 acgctggcgg gatctaacgt ctctttatat gaagaagtgt gtgccagata tccgcaagtg
     6241 gcgtttcagt cctccggcgg cattggcgac attaacgatg ttgctgccct acgtggcact
     6301 ggcgtgcgcg gcgtaatagt tggtcgtgca ttactggaag gtaaattcac cgtgaaggag
     6361 gccatcgcat gctggcaaaa cgcataatcc cgtgtctcga cgttcgtgat ggtcaggtgg
     6421 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgcca ctggcaaaac
     6481 gctacgccga agaaggtgca gatgaactgg tgttctacga tatcaccgct tccagcgatg
     6541 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgtttt
     6601 gtgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
     6661 ataaaatttc catcaactcc cctgcgctgg cagacccaac attaattact cgcctggccg
     6721 atcgctttgg cgtgcagtgt attgtggttg gtattgatac ctggtacgac gccgaaaccg
     6781 gtaaatatca tgtaaatcaa tataccggcg atgaaagccg cacccgcgtc actcaatggg
     6841 aaacgctcga ctgggtagag gaggtgcaaa aacgcggcgc cggagaaatc gtcctcaaca
     6901 tgatgaatca ggacggcgtg tgtaacggtt acgacctcaa gcagctgaaa aaagtgcgtg
     6961 aagtttgcca cgtcccgctg attgcctccg gtggcgcggg caccatggaa cacttcctgg
     7021 aagccttccg cgatgccgac gttgacggtg cgctggcagc ttccgtattc cacaaacaaa
     7081 taatcaatat tggcgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
     7141 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggtc tgatgccggt
     7201 gattgtgcaa cacgcggtat ctggcgaagt gttaatgctg ggctacatga acccggaagc
     7261 cttagacaaa accatcgaaa gcggtaaagt caccttcttc tcgcgcacta aacagcgact
     7321 gtggatcaaa ggcgaaacct cgggcaattt tctcaatgtg gtaagtattg ccccggactg
     7381 cgacaacgac acattactgg tgctggcgaa tcccatcggg ccaacctgcc acaaaggcac
     7441 cagtagctgc ttcggcaaca ccgctcacca gtggctgttc ctgtatcaac tggaacaact
     7501 gctcgccgaa cgtaaatatg ccgacccgga aacctcctac accgccaaac tgtatgccag
     7561 cggcactaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggcagc
     7621 aacggtacat gatcgctttg agctgaccaa cgaggcatct gacttgatgt atcacctgct
     7681 ggtgctgttg caggatcagg atctggattt aacgacagta attgagaacc tgcataaacg
     7741 gcatcagtga gttacgggct gagcaggtgt gatgttgttg tcacacccgg agccattgcc
     7801 cgatgcgcaa acttatcgtg cctacacatt atctcaacac acgtaggccg gataagatgc
     7861 gccagcatcg catccggcaa aaaaaacggg catggtgtca ccaccctgcc cgtttctctt
     7921 aaatgcacaa taatattact tcgcgttgta attacgcaga gcgttacgcc ccaacacaat
     7981 ccccgcgcca accatgccgc ccagaagcac tgccagaatc aaggtgattg cctttttcgg
     8041 actatcgcga cgaataggta acgtcggttt catcacatag cggtaagcat gaatatcaag
     8101 atcatcaaac tttaattttt caatatccaa caggttttga cgtgtctgat agtagtttgg
     8161 tgagaacacc aacggacgag tcgcttcatg cttaatcatc gactccagcg cttcgctccc
     8221 tagaaggaac aacgtatctt gcgtcacatc ttcagtctgc tgaacctgtg gctttgtcac
     8281 ctgcgcctga ttcgcatact gcaacgcttc ctgaatctga cggatacgca gatctttctg
     8341 ctcctgcgcg accacttcct gggttcttaa agagtcctgc aagtttttcc gtcccagagc
     8401 aatgttgtcc ttgagatcct tttctagctc ttgattcact ttatcatcaa cttgctgaat
     8461 gtattgggcc aacttcattt gtgcgccctc tgcagtttgc ccaacataag aaacagtcaa
     8521 tggtaattgc tggttcttaa cagaaggttc gatggtaagt ttttctggct cttcctgatt
     8581 atccagcgtt tctgctaatg cagagaaggc agaactgaag cgaccaatta acgtctcctg
     8641 caaatccgat actttcggtg cagcctgacc atagataaca ttcatggcat tgttatagcc
     8701 agcaatttgc cccacgtcgg gctgagtgat aattgctgtt gacgtccatt tctccttcgc
     8761 tactgccaaa taaccaatag ccagggcaat agccacaatg acggaaatga taattgtcat
     8821 cttgccacgc cacaactgca ctagtaaatc aatcaaatca atctgttccg ggtcatggtt
     8881 ttgcccagaa acattattat tttctactct catcattacc ctaactgacg gaaatttcca
     8941 agtccgcata gtttagcgat aatccccgtg aatgctatag gataagtgat aaaaataatg
     9001 aattattgcg gaggtaacaa aagacaatca gggcgtaaat agccctgata acaagatgtt
     9061 agtcgctgcc aaagagatcg cgggtgtaga ccttatctgc cacatcctta agctcttctg
     9121 ccatacggtt ggagataatg acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca
     9181 ggcgagagtt gaagaatgag tcttctttca tcaccggctc gtagatgatc acttcaacgc
     9241 ctttcgcctt gatacgcttc ataatcccct gaatagaaga cgcacggaag ttatctgacc
     9301 cgctcttcat aatcagacga taaataccca ccacttgcgg tttgcatcga caattgccga
     9361 gatcaggtta ttcggcacag actggtagtt cgccagtaac tgcttggtat ctttcggcag
     9421 acaataacca ccataaccaa acgacggatt gttgtaatgg ttgccaatac gcggatcgag
     9481 acaaacgcct tcgataatct ggcgagtatt cagacctaaa ctttctgcat aactatccag
     9541 ttcattaaag tatgctacgc gcatcgccag ataggtatta gcgaaaagtt taatcgcttc
     9601 tgcttcagtg gagtcggtaa acagggtcgg gatattttgc ttaatcgcgc cttcctgtaa
     9661 caacgcagca aaacgttctg cgcgttctga acgctcaccg atgacaatac gtgacggatg
     9721 gagattatcg taaagggctt taccttcgcg gaggaattcc ggagagaaaa taatattttc
     9781 agtacgatat ttcttatgca tcgctgcggt aaaaccaacg ggaaccgttg atttgatgac
     9841 cataaccgca taaggattta tctcaactac gtctttaatt actgattcta cactggatgt
     9901 attgaaataa ttagttttag gatcatagtc ggttggagtg gcgatgatga cataatcagc
     9961 atcccggtag gcttcatttt tatctaatgt ggcattaaag tgtattttat ctgattgcaa
    10021 aaactgctga atttccttat caacaatagg agatatccga tcattcagca tagcaacgcg
    10081 tgacggtaaa atatctaatg ccacaacctc atgattttgt gcgattagaa gcccgtttga
    10141 caagcctaca tagccagtac cggaaatggt gattttcatt tcatgctctc agaattaact
    10201 taactgtgaa tcatgatgtt tttagcatcg tgataagatg ctaaaagttt taacgctacg
    10261 ttgtcagatg gtcaacgcaa cgaaaaacaa ttcagatttt ctttataaga atagctgatg
    10321 tttattaagt taatattaag cagtgaaatt tagtctataa gatatttggc aaaaaaaagc
    10381 ccggtgcaat atacgccggg cctcaatttt attgttggtt aaatcagatt aatccagcca
    10441 ttcagtatgg aacacacctt ctttatcaat gcgcttataa gtatgcgccc cgaaatagtc
    10501 acgctgtgcc tggatcaggt tcgcaggcag aaccgcggca cggtagctat cgtaataggc
    10561 aaccgcagcg gcgaaggtcg gaaccgggat accgttctgt actgcataag cgacgacatc
    10621 gcgcagcgcc tgctggtagt catcggcaat ttgcttgaag tacggggcca gcagcaggtt
    10681 agcgatctgc ggattttcgg cataagcatc ggtgattttc tgcaggaact gcgcacggat
    10741 gatgcagcca gcacggaaaa tcttcgcgat ttcgccgtag ttcagatccc aattgtattc
    10801 ttcagacgca gcacgcagct gagagaagcc ctgagcgtaa gaaacgattt tgcccagata
    10861 cagcgcactg cgaacttttt cgatgaactc agccttgtcg cctgctgact gagcttgcgg
    10921 accagagaga actttagatg cggcaacgcg ctgatctttc agagaagaga tataacgtgc
    10981 aaacacagac tcggtaatca gcgacagcgg ttcgccgaga tccagcgcgc tctggctggt
    11041 ccatttaccg gtgcctttgt ttgctgcttc atccagaatc acatcaacca ggtagttacc
    11101 gtcttcatct tttttggtga agatatcttt agtgatgtcg atcaggtagc tgctaagttc
    11161 accgttattc cactcggtga aagtctgggc cagttcttca ttagatagat tcaggccacc
    11221 tttgagcaga gaatacgctt cagcaatgag ctgcatatct ccgtattcaa taccattgtg
    11281 aaccatcttc acataatggc ctgcgccgtc ggcaccaata taagtaacgc atggctcgcc
    11341 gtcttctgcc acggcagcga ttttagtcag gataggcgca accagctcat aagcctcttt
    11401 ctgcccgcca ggcataatag aaggaccttt cagcgcgccc tcttcaccgc cggaaacacc
    11461 ggtaccgatg aagttaaagc cctctgctga aagctcacga ttacgacgaa tggtgtcctg
    11521 gaagaaggta ttaccaccat caatgatgat gtcaccttta tcgagatatg gtttcaggga
    11581 atcaatcgca gcatccgtgc ctgcacctgc tttcaccatt aacaggatgc gacgaggcgt
    11641 ttccagagat tcaacaaact ctttcaccgt atagtaagga gccagtttct tgcctggatt
    11701 ttcagcaatc acttcttccg tcttttcgcg ggagcggttg aaaatagaga cggtataacc
    11761 acgactttcg atgttgagcg caaggttgcg ccccatcact gccataccga ctacgccgat
    11821 ctgttgcttt gacattgttt actcctgtct tctatattat taaccgtcta ttaaccgtta
    11881 tattattaac cgtcgtgatt ttcatcttct aatcaaggaa tacatatggt tacagcatgc
    11941 ttcttacttt atagcgataa gtatgccatt gggtgtatcc ataagaactt tgaattttgg
    12001 atacttttct ggattaaaat tagcatagct tcgtttagat ataacaataa acgaactatt
    12061 aaaagatttc atcttctcta ttaaatcatc ctgtgggatt acctcacact cgggattact
    12121 aatagtccaa tcactatatc ttgctaatcc aaagacgtca ccaattgcca tccctttata
    12181 gaagtaaata ccatcctcca agcctaattg gataagatta tctccatata actcactata
    12241 ttcacttgct ttcttcatca gttcatagct atagcgactt tctaagcgtg aattaaaatt
    12301 caagtattca gaataaaaat atttgaattc aaaatacaac gatcgccccg aaaaaaaata
    12361 atacacacaa ggataaaata tcgtcccaac ttgtaatttg taatgttgat atcttttatt
    12421 aaacttgcaa ttgagatcgc aacctgcaat actgacaatg ggtaaatggg tgataaatac
    12481 ctgtcacctt gcgtaacaga aaacca
//
LOCUS       DAZSIO010000115        12343 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZSIO010000115 DAZSIO010000000
VERSION     DAZSIO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.115"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            758..841
                     /gene="dinQ"
                     /locus_tag="ACHX5T_002922"
     CDS             758..841
                     /gene="dinQ"
                     /locus_tag="ACHX5T_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR8468022.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(895..2247)
                     /gene="gorA"
                     /locus_tag="ACHX5T_002923"
     CDS             complement(895..2247)
                     /gene="gorA"
                     /locus_tag="ACHX5T_002923"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR8468023.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="ACHX5T_002924"
     CDS             complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="ACHX5T_002924"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR8468024.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3364..5406
                     /gene="prlC"
                     /locus_tag="ACHX5T_002925"
     CDS             3364..5406
                     /gene="prlC"
                     /locus_tag="ACHX5T_002925"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR8468025.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5414..6166
                     /gene="rsmJ"
                     /locus_tag="ACHX5T_002926"
     CDS             5414..6166
                     /gene="rsmJ"
                     /locus_tag="ACHX5T_002926"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR8468026.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6215..7683)
                     /gene="dtpB"
                     /locus_tag="ACHX5T_002927"
                     /pseudo
     CDS             complement(6215..7683)
                     /gene="dtpB"
                     /locus_tag="ACHX5T_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            7849..7902
                     /locus_tag="ACHX5T_002928"
     CDS             7849..7902
                     /locus_tag="ACHX5T_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468027.1"
                     /translation="MVLNFVLAGDFDCVRRS"
     gene            complement(8001..8435)
                     /gene="uspA"
                     /locus_tag="ACHX5T_002929"
     CDS             complement(8001..8435)
                     /gene="uspA"
                     /locus_tag="ACHX5T_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR8468028.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8829..9164
                     /gene="uspB"
                     /locus_tag="ACHX5T_002930"
     CDS             8829..9164
                     /gene="uspB"
                     /locus_tag="ACHX5T_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR8468029.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9235..10734)
                     /gene="pitA"
                     /locus_tag="ACHX5T_002931"
     CDS             complement(9235..10734)
                     /gene="pitA"
                     /locus_tag="ACHX5T_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR8468030.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10966..12165
                     /locus_tag="ACHX5T_002932"
     CDS             10966..12165
                     /locus_tag="ACHX5T_002932"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR8468031.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3024 a         3127 c         3307 g         2885 t
ORIGIN      
        1 taacagaaat gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga
       61 taagtcaaaa acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg
      121 aattttgagt tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact
      181 ttgcgtcggt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
      241 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
      301 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
      361 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
      421 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
      481 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
      541 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
      601 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
      661 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
      721 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
      781 tcttggggcg ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
      841 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
      901 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
      961 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     1021 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     1081 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     1141 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     1201 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     1261 cttgttatta aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     1321 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     1381 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     1441 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     1501 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     1561 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     1621 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     1681 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     1741 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     1801 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     1861 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     1921 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     1981 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     2041 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     2101 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     2161 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     2221 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     2281 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     2341 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     2401 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     2461 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     2521 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     2581 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     2641 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     2701 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     2761 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     2821 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     2881 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     2941 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     3001 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     3061 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     3121 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     3181 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     3241 aggccgccgc cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc
     3301 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     3361 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     3421 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     3481 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     3541 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     3601 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     3661 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     3721 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     3781 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     3841 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     3901 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     3961 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     4021 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     4081 agcacccgcg cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg
     4141 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     4201 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     4261 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     4321 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     4381 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     4441 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     4501 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     4561 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc
     4621 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     4681 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     4741 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     4801 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     4861 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     4921 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     4981 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     5041 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     5101 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     5161 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     5221 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     5281 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     5341 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     5401 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     5461 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     5521 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     5581 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     5641 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     5701 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     5761 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     5821 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     5881 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     5941 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     6001 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     6061 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     6121 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     6181 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     6241 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     6301 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     6361 ccggcaccgc ggtaaatgtt gccacatagc cgccagcaag aacgcggcag cctgcgtcag
     6421 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
     6481 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
     6541 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagccgt
     6601 caaaaagccc agtgagcaca taaacatgct gagagtaaat ttcatcggca tcgagaggtc
     6661 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
     6721 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
     6781 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
     6841 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
     6901 atccagcttg aatgcctgac gaaagaagat gatagtgacg acgatggaga gaacaatcag
     6961 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
     7021 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
     7081 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
     7141 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
     7201 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
     7261 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
     7321 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
     7381 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
     7441 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
     7501 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
     7561 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
     7621 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
     7681 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
     7741 gaaaaagttt acatctgtca gagattctta atattgatgc ggtgaataga ttaatacact
     7801 attacaacag aaaataacca gctgatcaca ctaatgatgg aaaatatgat ggtgttaaat
     7861 tttgtattgg caggagattt tgattgcgta cgcagatcgt aggccagata aggggtttac
     7921 gctgcatcag gtaacaaggc gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg
     7981 aacacgtcgt ggagggatga ttattcttct tcgtcgcgta gcggaacaat cagcatatca
     8041 acgtgaacgg tgttgatcag ctgacgtgcg gaagacatca gtttgctcca gaagtcctgg
     8101 tggtgaccac aaaccaccag atccatatcg tatttcttga ttgcatcgac cagaacctgg
     8161 cctaggtcgc cgctgccgct cagggtttca gtgattggat agcctgcatt agtggaaagc
     8221 tcggtcagcg catgatgtgt ctcttcagag atgcgtttct gcatatcacc cagattcaca
     8281 tcaataagcc cggtgtatag gtcagagtag tttacatcta cgtggatcag agaaactttc
     8341 gcattgtagg ggcgagccat agagactgct ttctctacca gaactttgct ttccggggag
     8401 agatcgactg cgatgagaat gtgtttataa gccatagtgt tactccttcc ataaagttgt
     8461 cgatgactgg ccagctagcg tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg
     8521 tgcgcggggc taacccagcc gtaaagattc agtgttaaga ccatccttac cttatagcga
     8581 cccggatgat ccgtcaatcc gccttgctta cccgttagat aaacaaattt tcgacatgaa
     8641 tattatattg atagtggtta accttctgga aaaaaaacaa cctgatctcc tacactatct
     8701 atagagccgc tcggatgttg gtcaccacaa aagcggtttc tccaggacct ggtgttaact
     8761 gattggcagc gtatcgggag cggtagcccc ggggtggaac tccgcgggca ggtcgccggg
     8821 gaggaggtat gataagcacc gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta
     8881 acatggcgcg ctatttctca tcactacgcg cgttgttagt ggtactgcgt aactgcgatc
     8941 cattgctcta tcaatatgtt gatggagggg gcttttttac ctcacatggc caacccaaca
     9001 aacaggtgcg tctcgtttgg tatatctatg cccaacgtta tcgcgatcat cacgatgatg
     9061 agtttattcg ccgctgtgag cgggtgcgtc ggcagtttat tctgactagc gcattgtgtg
     9121 gtctggtggt ggtcagcctg attgcattga tgatttggca ttaagcactt cagcaataat
     9181 aacgtataaa aaaagcgggc cagtaaactg acccgctcgt tttggtgcgt acgattacag
     9241 gaactgcaag gagaaccagt acagcccgcc ggaaagtagt accgcagccg gaagggtaaa
     9301 cacccaggcc atcagaatac tggttacggt tttacgctgt aagccgccgc catctaccac
     9361 catcgtcccc gcgacagaag aggagagtac gtgagtggtg gaaaccggca tcccggtata
     9421 actcgccagg ccgattgaca ctgccgccgt catctgggca gacatcccct gagcgtaggt
     9481 catgcctttc ttaccgattt tctcaccgat agtcgttgcc acacgacgcc agccaatcat
     9541 cgtaccgata cctaacgcca gcgcgaccgc catgatgatc cacaccggcg catactcgat
     9601 ggtgctaagc atgtcggact tcagtttctt caacaggcgc tgatcgtcag cactcacacc
     9661 aggcatcttc accactttgt cgatagtgtc agaaacgcac agcataatgc ggcgcatctg
     9721 gctacgttga tcaagcgaca gcttgtcgta gctttccaca tcggtggtca acataccttt
     9781 caggcggttg agcgcgttaa tggtattcga cggatggcag tggaactccg caggttgcgt
     9841 tgcgccagct tccggagccg gtactaactg atcagcaccg gtagcctgtt tgagcagcgc
     9901 aggatgctgc tcaaagtaag cttcgacgtt gttgatggca tcacgggtac gggtgatttc
     9961 gtagccagtg gcattcatgt tcaccacgaa gcctgctggc gcgacgccaa tcaataccaa
    10021 cataaccaga ccaatgcctt tctgaccatc gttcgcgccg tgcgaaaacg ccacgccgat
    10081 agcggaaagg atcagcgcga tacgcgtcca gaacggcggc tttttcttgc cgtctttctt
    10141 ttcacgctcc gctggggtca ggtggatacg ggcgcgtttc ttggtgccgc tccagtagcg
    10201 acgcagcaag aaaatcagac cgccagcaaa caccaggccg acaatagggg aaacgatcag
    10261 agaaccgaaa atacttaata ctttcgggat attgagtgca tccaccactg acgtcccggt
    10321 catcaacgca ttggttaaac caatcccgat gatcgcgcca atcagcgtat gagagctgga
    10381 tgcaggtaaa ccaaagtacc aggtacccag gttccagata atcgccgcca gcaacataga
    10441 gaacaccatg gcaaggccat gagacgatcc catattaagc agcagatccg tcggcagcat
    10501 atgcacaatg gcataggcaa cactcagacc acccagcaaa acacccaaaa agttgaacac
    10561 cgccgccata accacggcga gctgagaacg catcgcgcgg gtatagataa cggttgccac
    10621 ggcgttggct gtgtcatgga aaccattgat ggcttcgtag aacagcacaa aagccagtgc
    10681 aagcaataat aacagcccgg tatgcaaatc caggccagca aacaaatgta gcataggacg
    10741 ttacgccatt ttgaggacat gaacgcggcg cattatcagt gactttcgcg gcgcgggcaa
    10801 agtgaaatat agactttttt tgatttgcct cctgtatgga tttcactcaa aaaataatta
    10861 tcttatataa ttcaggcaaa tacttccttt tagtaatatt gatgctggtg cgaccactga
    10921 ggaatcttta caattcacgc ccgttttttc taagaggagc gcaacgtgga aaggtttgat
    10981 gccattatta taggcgctgg tgcggcgggt atgttctgtt ctgcgctggc aggtcaggta
    11041 ggacgccggg ttctgctgat cgataatggt aaaatactag ggcgcaaaat ccttatgtct
    11101 ggcggtgggc gctgcaactt taccaacctt tatgtcgaac caggcgctta tctgagccag
    11161 aatccgcatt tttgtaagtc tgcactcgca cgttttaccc agtgggattt cattgatctg
    11221 gtcaataaac acggtatcgc ctggcacgag aaaacgttag ggcaactctt ctgcgatgac
    11281 tccgcgcagc agattgtcga catgctggtg gatgagtgcg agaagggcaa tgtaaccttc
    11341 agattgcgta gcgaagtgct gagtgtggcg aaggatgaaa caggcttcac gcttgatctg
    11401 aacggcatga ctgtcggttg cgaaaagctg gtcatcgcga ccggtgggct gtcaatgccg
    11461 gggctgggcg catcgccgtt tggttataag attgccgaac aatttggcct caacgtgctg
    11521 ccgacccgcg cgggtctggt gccattcact ctgcataaac cgttgctcga agagttacag
    11581 gtgctggcgg gcgtggcggt gccttccgtg attaccgctg aaaacggcac cgttttccgt
    11641 gagaacttac tcttcaccca ccgcggctta tctggaccgg cggtgttgca gatttcaagc
    11701 tactggcaac cgggtgaatt tgtcagtatc aatctgctac cggatgtgga cctcgaaacc
    11761 ttcctgaatg agcagcgtaa cgcacatccg aatcaaagcc tgaaaaacac actggcggtt
    11821 catctaccga agcggttggt tgaacgctta cagcaactcg ggcaaatccc ggatgtttcg
    11881 ctaaaacagc tcaacgtgtg tgaccaacag gcactgatta gcacattgac cgactggcgc
    11941 gtacaaccca acggcactga aggctatcgc actgccgaag tgacgctcgg cggcgtggac
    12001 accaacgaac tttcttcacg gacgatggaa gcgcgcaaag tgcctgggct gtacttcatc
    12061 ggtgaagtga tggacgttac cggctggctg gggggctata acttccagtg ggcgtggtcg
    12121 agtgcatggg cttgtgcgca ggatttggcg tcaattgtga aataaccatg acgttttccc
    12181 gtcagccgtt tgtatatgtc gttattaagt cattttggta cggttctgca tccgacattc
    12241 tggtgttaac tcgctgtttt gttagcgaat taacgctatt gccgttgtgg ttattttata
    12301 gtgtatttat tctcagagaa tatatattac tgaaataaaa aac
//
LOCUS       DAZSIO010000116        12211 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZSIO010000116 DAZSIO010000000
VERSION     DAZSIO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.116"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            271..1041
                     /locus_tag="ACHX5T_002933"
     CDS             271..1041
                     /locus_tag="ACHX5T_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR8468032.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1032..1505
                     /locus_tag="ACHX5T_002934"
     CDS             1032..1505
                     /locus_tag="ACHX5T_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR8468033.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1612..2151
                     /gene="dnaT"
                     /locus_tag="ACHX5T_002935"
     CDS             1612..2151
                     /gene="dnaT"
                     /locus_tag="ACHX5T_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR8468034.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2154..2891
                     /gene="dnaC"
                     /locus_tag="ACHX5T_002936"
     CDS             2154..2891
                     /gene="dnaC"
                     /locus_tag="ACHX5T_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR8468035.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2940..3434
                     /gene="yjjA"
                     /locus_tag="ACHX5T_002937"
     CDS             2940..3434
                     /gene="yjjA"
                     /locus_tag="ACHX5T_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR8468036.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3674..5965
                     /gene="opgB"
                     /locus_tag="ACHX5T_002938"
     CDS             3674..5965
                     /gene="opgB"
                     /locus_tag="ACHX5T_002938"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR8468037.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6103..7125)
                     /gene="lgoD"
                     /locus_tag="ACHX5T_002939"
                     /pseudo
     CDS             complement(6103..7125)
                     /gene="lgoD"
                     /locus_tag="ACHX5T_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7264..8178
                     /locus_tag="ACHX5T_002940"
     CDS             7264..8178
                     /locus_tag="ACHX5T_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR8468038.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8393..9754
                     /locus_tag="ACHX5T_002941"
     CDS             8393..9754
                     /locus_tag="ACHX5T_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8468039.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9803..11467)
                     /gene="tsr"
                     /locus_tag="ACHX5T_002942"
     CDS             complement(9803..11467)
                     /gene="tsr"
                     /locus_tag="ACHX5T_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFR8468040.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11586..12032)
                     /gene="hpaR"
                     /locus_tag="ACHX5T_002943"
     CDS             complement(11586..12032)
                     /gene="hpaR"
                     /locus_tag="ACHX5T_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR8468041.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
BASE COUNT         2993 a         3208 c         2940 g         3070 t
ORIGIN      
        1 ttaacttgtt aacactaaaa aacgcgccca gctatctatt atcctgaccc gtaaaggcta
       61 aacaataaat tcattatagt gataatattc atgtgatagt ttgtttgcgg cgagagataa
      121 ttcgcttttt atcaccgagc ttgtcccaat aaccaggatt aatcagcgca attttgatct
      181 taacctaccg caaaacaacc gtgcttcatg atacaaatgg attctcatta tctgaacaat
      241 acgcagcacg tctatgacaa agggcgagtt atgcaaactg agcaacagcg agccgtaacg
      301 cggctttgta tccagtgtgg attatttctt ttgcaacatg gtgcggaaag cgcgttggtt
      361 gatgagcttt cctcacgact gggtcgggcg ctgggaatgg acagcgtcga aagttctatc
      421 tcttcgaacg ccatagtgct gacaactatt aaagatgggc aatgcctgac atcgacacgt
      481 aaaaatcacg atcgcggcat taatatgcat gtggtgactg aagtccagca cattgtgatt
      541 cttgcggagc atcatctgct ggattacaaa ggcgtagaga aacgatttag ccaaattcaa
      601 ccattacgtt acccaagatg gctggtagcc ttaatggttg gcctttcttg cgcctgtttc
      661 tgtaaactca ataacggtgg ctgggatggt gccgtcatca ccttctttgc cagtacgacc
      721 gcgatgtata tccgccagct gctggcacaa cgtcatcttc atccacagat caacttttgc
      781 cttaccgctt tcgccgccac caccatttcc ggattgcttt tgcaactccc cactttcagc
      841 aataccccca ccattgcgat ggccgccagc gttctgctgc tagtgccggg ctttccgttg
      901 attaatgccg tcgccgatat gtttaaaggc cacatcaata ccggactggc acgctgggcg
      961 atcgccagtc tgctgacact ggctacctgc gtcggcgtag tgatggcact gacgatttgg
     1021 gggctacgcg gatgggtgtg atcgaatttc tgttagcgtt ggcgcaggat atgatcctcg
     1081 ccgccattcc tgcggtcggc tttgcgatgg tgttcaacgt tcccgtgcgg gcgttacgct
     1141 ggtgtgcgct gcttggctcg ataggtcatg gttcccgaat gatcttgatg accagcgggt
     1201 tgaatattga gtggtcaacc tttatggctt ctatgctggt cggtaccatt ggtattcaat
     1261 ggtcgcgctg gtatctggcg catccgaaag tgtttaccgt ggcggccgtt atccctatgt
     1321 tcccgggcat atcggcttat accgcaatga tttcgtcggt aaaaatcagc cagttaggtt
     1381 acagcgaacc gttgatgatt accctgttaa ccaactttct tacagcttca tcgattgttg
     1441 gtgcgttatc catcggtctt tccattcctg gattatggtt gtaccgcaag cgccctcgcg
     1501 tataaaattg cctcgcttaa gcacacggat gagagacagc ctcctctcct ccgtgtgtta
     1561 ctataaaagt tatctccctt ctcgttcatc gttccatatt tgagaaacag tatgtcttcc
     1621 agagttttga ccccggacgt cgttggtatt gacgccctgg tacacgatca ccaaaccgtt
     1681 ctggcaaaag ctgaaggcgg tgtggttgcc gtatttgcta acaatgcccc ggcgttttat
     1741 gccgtcacgc ctgcacgcct ggctgaactg ctggcgctgg aagaaaagct agcgcgtccg
     1801 ggaagcgatg tcgctctgga cgatcaactc tatcaggaac cgcaagccgc tcccgttgct
     1861 gtacccatgg ggaaattcgc catgtatccg gactggcaac ccgatgccga ttttatccgc
     1921 ctggcggcgc tatggggcgt ggcgctaaga gagccggtga ccaccgaaga actggcctca
     1981 ttcattgcct actggcaggc ggaaggtaaa gtctttcacc atgtgcagtg gcaacaaaaa
     2041 ctggcgcgca gcctgcaaat cggtcgtgcc agcaacggcg gactgccgaa acgagatgtg
     2101 aatacggtca gtgaacctga cagccaaatt ccaccaggat tcagagggta acgatgaaaa
     2161 acgttggcga cctgatgcaa cgcctgcaaa aaatgatgcc tgcccatatc aaacctgcat
     2221 tcaaaacggg tgaagagcta ctggcctggc agaaagaaca aggggcgatc cgctccgccg
     2281 ctctcgaacg tgaaaatcgg gcgatgaaaa tgcagcgcac ctttaaccgc tccggtattc
     2341 gtccactgca tcagaactgc tcctttgaga actatcgcgt tgagtgtgaa gggcagatga
     2401 atgcgttaag caaagcgcgc cagtatgtcg aagagtttga cggcaacatc gccagcttca
     2461 tcttttctgg taagccagga accggcaaaa accatctggc ggcggcaatc tgcaacgagc
     2521 tgctgctacg cggtaaatcc gtattgatca tcaccgtggc cgatattatg tcggcgatga
     2581 aagatacctt caggaatagc ggtaccagcg aagaacaact gcttaacgat ctgagcaacg
     2641 ttgatctgct ggtgatcgat gagatcggcg tgcagaccga atcgaaatac gaaaaagtga
     2701 tcatcaacca gatcgtcgat cgccgctctt cttccaaacg cccaaccggg atgctgacca
     2761 acagtaatat ggaagagatg accaagttgc tgggcgaacg cgtgatggac cgtatgcgcc
     2821 tgggtaacag tttatgggtg atcttcaact gggatagcta ccgtagccgg gtaacgggta
     2881 aagagtatta atccccgttt ctggcggtat actgcgacac aattccacct tgagtaatga
     2941 tgatgaaaac tattaaacat cttctgtgct gtgccattgc cgccagcgca ttaatttcca
     3001 ccggggtgca tgctgcgtcc tggaaagatg cgctctccag cgccgccagc gaacttggca
     3061 accaaaacag cacgacacag gaaggcggtt ggtcgctcgc gtcattaact aacttgctta
     3121 gcagcggaaa ccaagcctta agcgcagata acatgaacaa tgccgcaggc attctgcaat
     3181 actgcgcgaa gcaaaagctg gcttcggtaa ccgatgccga aaacatcaag aaccaggtgc
     3241 tggaaaagct gggcctgaac agtgaagagc aaaaagaaga caccaactat ctggacggta
     3301 ttcagggttt gctgaaaaca aaagatggtc agcaactcaa tctggataac atcggaacga
     3361 ctccgctggc agaaaaggtg aaaaccaaag cctgcgatct ggtgttaaag caggggctga
     3421 acttcatttc ctgaaccgcg aaatgttacc cggctgacgc ggctctctcc tgcccttttc
     3481 cctgctcctg gacggttttc cccctgttac acgggaagcg accgcgatca aaaaagtctg
     3541 tttataaact aattcctaac aacatcgcat ttttttgaca ctagaattgc agcaatatgg
     3601 cgacgccatt acattgtgct tctcaaaaag cttaaacgtt ggctaaacgg cccggtttag
     3661 tgaggatcgc ccattgtcag aactactctc tttcgccctt tttctcgcct ctgtgctgat
     3721 ttacgcatgg aaagcgggac gtaacacctg gtggtttgca gccacgttaa cggtgctggg
     3781 gctatttgtc gttttaaata tcaccctgtt tgccagcgac tattttactg gcgatggtat
     3841 taacgacgcg gttctctata ccttaaccaa cagcctgacc ggtgctggcg tcagcaaata
     3901 catcctgccg ggtatcggca ttgtgctggg gctgacggcg gtgttcggtg cgctgggctg
     3961 gatcctgcgc cgtcgtcgtc atcatccgca ccattttggt tacagcctgc tggcgctctt
     4021 actggcgctg ggttcggtgg acgccagccc ggcatttcgt cagataacgg aactggtgaa
     4081 atcccagtca cgcgatggcg acccggactt tgcggcttat tataaagagc cgtcgaaaac
     4141 tatccctgac ccgaaactca acctggttta tatctacggc gaaagtctcg agcggaccta
     4201 ttttgataac gaagctttcc cggatctcac gcctgaactg ggcgcgttga aaaatgaagg
     4261 cctggatttc agccacacgc agcagctgcc aggaacggat tacacgattg cgggcatggt
     4321 ggcttctcag tgcggcatac cgctgttcgc cccctttgaa ggcaacgcct ccgcctctgt
     4381 ctccagcttc ttcccgcaga acatctgtct gggcgatatc ctgaaaaact cgggttatca
     4441 gaactatttc gtgcagggcg caaatctgcg ttttgccggt aaagatgtgt tcctgaaatc
     4501 gcacggcttc gaccacttat acggctcaga agagctgaaa agcgtggtag ccgacccgca
     4561 ctatcgcaac gactggggat tctacgacga taccgttctc gatgaagcgt ggaaaaagtt
     4621 tgaagagctt tcccgctcag gtcagcgatt ctcactgttt accctgacag tcgataccca
     4681 tcacccggat ggttttatct cgcgcacctg taaccgcaaa aaatatgatt ttgacggtaa
     4741 gccgaatcag tcattcagcg cggtaagttg cagccaggag aatatcgcga cgtttatcaa
     4801 caaaatcaaa gcgtcaccgt ggtttaaaga tactgtcatc gtcgtctctt ctgaccattt
     4861 agcgatgaac aacacggcgt ggaaatacct caataagcag gatcgcaata acctgttttt
     4921 tgtcattcgt ggcgacaagc cgcagcaaaa gacgctggca gtgaagcgta acacgatgga
     4981 taacggcgcg acggtgctgg acattctcgg tggcgataac tatctcggac ttggtcgtag
     5041 cagtttatcc gggcagtcga tgtcggaaat cttcctcaat atcaaagaga aaacattggc
     5101 gtggaagcca gatatcatcc gcctgtggaa attccctaaa gagatgaaag agttcaccat
     5161 cgaccagcag aaaaacatga ttgccttctc gggtagccat ttccgtttgc cgctgctgtt
     5221 gcgggtttca gacaaacgcg tggaaccgct gccggaaagc gaatactcag caccgctgcg
     5281 tttccagctg gccgatttcg ctccacgcga caatttcgtc tgggttgaca gttgctacaa
     5341 gatggcacaa ctctgggctc cggaactggc actttccacc gactggtgtg tctcgcaggg
     5401 gcagcttggc ggtcagcaaa ttgttcagca tgttgacaaa acaacatgga agagcaagac
     5461 ggcatttaaa gatacggtca tcgacatggc gcgttacaaa ggcaatgtcg atacgctgaa
     5521 gattgttgat aacgatattc gctacaaagc cgacagtttc atctttaacg tcgccggtgc
     5581 gccagaagag gtgaaacagt ttagcgggat ttcccgtccg gagtcgtggg gccgctggtc
     5641 caacgcgcag ctgggcgatg aagtaaaaat cgagtacaag catccgctgc cgaagaaatt
     5701 tgacctggtg attaccgcca aagcatacgg caataacgcc agccgtccta ttccggtacg
     5761 cgtaggcaat gaagaacaaa ctcttgtgct gggcaatgaa gtgaccacca ccacactgca
     5821 tttcgataac ccaaccgatg ccgacacact ggtaattgtg ccgccggaac ctgtctcaac
     5881 caacgaaggg aatatcctcg gacactcgcc gcgtaagctc gggatcggca tggtggaaat
     5941 taaagtggta gaacgtgaag ggtaattcct gactgatgtg taggtcgcat agggtgcgac
     6001 ctacacgcct tctccggctt acaacacatt attaagaaac aaaaaaaaca gcgcccaaca
     6061 cttccccgga cgctgcatgg caactttaat aatctcgcct tatcagaaag taattacacc
     6121 cttaatcaac tcacgattgt taatcacatc gcgctcgtag gtttctgcca gcgtggcgaa
     6181 cggatagcga tgggttaaca tcatgtcggc agtgattttc ccttccgcca tcagtcgacc
     6241 gactttagcg aaatcttccg gcgtggcgtt gcggctgccc atcatcgtcg tttctttttt
     6301 atggaattcc ggatcggaga actgcaactc acctttaaac agaccgacaa ataccaccgt
     6361 gccgccgtga cgaatcagat tcacggtatt attcatcgca tgttgattac ctgtcgcgtc
     6421 gatcactttc tgcgccagcg aaccaccaaa ctgcgcccgc agctgcgcgt caaaatcttc
     6481 ggctgacggg tccagcacgg gcaactccag acgcgttgcc acatgttcac ggcgcgccgg
     6541 actggtatcc gccaccacca cctgtgcgcc atcggcttta gcgattgccg ccgcgcccag
     6601 accgattggc cccgccccga ccaccagcac ctgctcgccg ggagcaatgg ctgcgcgacg
     6661 caccgcatga gcgctaatag cgaaaggttc aatcaatgcg gccgcctgcg ggtcaatacc
     6721 gtctgcgggc aaaatgttcg ccaccggcac gctcaaatac tcactaaaac cgccatcctg
     6781 atgcacgcca atgactgaaa ttttttcaca gcagttggta cgcccgcttt tacacgccgg
     6841 gcattgctga caggcaacat aagggatcac agcaacttgc tgaccatttt taagatcagc
     6901 aatattttta cccagcccaa caatctcccc acatatttca tggcctaaaa cccgtggata
     6961 actaaaaaat ggttgatttc caccctaggc atgaatatcg gtaccgcaaa tcccgacaga
     7021 cttaattttt attaatgctt cattgtcacc cggaataggt atctcgcgtt gtttccagac
     7081 taattctttc ggctgctggc aaattaaaac attcatcgta gacatgattc ggctatccat
     7141 tttttgttgc cttagatatc aatgaaaatg aaaaatggca gcggaacgga aaatctgttt
     7201 tgtgaaaaca cacgcataaa aatgttttaa atcgggtttt aatggactca caaggaggaa
     7261 aatatgagtc gttcacaaaa tttacgccac aatgtgatta accaggtcat tgatgatatg
     7321 gcgcggggtc atatcccttc cccacttcca tcgcaaagtg cgctggcgga aatgtacaac
     7381 atcagccgca ctacagtgcg tcatattctc agccaattac gcgaatgcgg cgtcttaacg
     7441 caggttggaa acgactatgt tattgcgcgt aaacctgacc atgatgacgg tttcgcctgt
     7501 actaccgctt caatgagtga acaaaacaaa gtgtttgagc aggctttttt cacgatgata
     7561 aaccagcgtc agttacgccc tagggaaact ttttctgaac tgcaactggc tcgggccgcg
     7621 ggtgtcagcc cggtggttgt aagagaatat cttttaaaat tcggccgtta taatcttatt
     7681 caaagcgaaa agcgcggcca gtggagcatg aagcaattcg atcaatccta cgccgagcag
     7741 ctgtttgagc tacgcgaaat gctcgaaaca cattcacttc agcactttct caacctgcct
     7801 gatcatgatc cccgctggct gcaggcgaaa accatgctag aacgtcatcg gctactgcgt
     7861 gacaacatcg gcaatagctt tcgcatgttc tcgcaacttg acagagattt tcactcctta
     7921 ctgctttcag ctgccgacaa tatctttttt gatcaatcgc ttgagatcat ctcagtcatc
     7981 tttcattttc actatcaatg ggacgaaagc gatcttaaac agcgcaatat cattgccgtc
     8041 gatgaacata tgaccattct cagcgctctg atttgccgca gtgaccttga tgccaccctt
     8101 gcgctccgta accatttgaa ttcagctaaa caatcaatga ttcgatccat taatgagaac
     8161 acgcgttatg ctcattaagt acggattaaa aacagcaaac tattagcggg tgcacggttt
     8221 tatcaaaaga taaataccgg gaaattcccg cttacctatg ctcacaatca gtcgactaaa
     8281 aatgtttttg tgagcggtag taaagtccta aaactttaac ctgccgttaa tgtcaacctt
     8341 gcgtaatgca caattacgta aacaattaaa attataaata caggagtccg gcgtggaaaa
     8401 agaaaatatc accctcgatc cgcgttcttc atttactcca tcttcgtcgg cagatattcc
     8461 cgtgccacca gatggattag ttcaacgcag tacccgaatt aaacgcattc aaaccaccgc
     8521 catgttgtta ttattttttg cggcggtaat caattatctc gaccgcagtt cgctgtcggt
     8581 agcaaattta acgattcgtg aagaattggg attaagtgcc accgaaatcg gcgctttgct
     8641 ctccgtgttt tcactcgctt acggcattgc gcaacttcct tgcggcccac tattggatcg
     8701 taaaggccca cgcctgatgc tgggactggg gatgttcttc tggtcactgt tccaggccat
     8761 gtctggcatg gtgcacagct ttacgcagtt cgtgttggtg cgtatcggta tggggattgg
     8821 tgaagcgccg atgaacccat gcggtgtaaa agtcattaac gactggttca acatcaaaga
     8881 gcgcggacgc ccgatgggct tcttcaacgc agcttctacc attggcgttg ctgtaagccc
     8941 accgattctg gcggcaatga tgctggtgat gggctggcgc gggatgttta ttaccattgg
     9001 tgtactgggg atttttctcg ccatcggctg gtatatgctc tatcgcaacc gcgagcacgt
     9061 agaactgact gccgttgaac aagcttatct caatgcaggt agcgtcaatg cccgccgaga
     9121 tccgctcaat tttgccgaat ggcgcagcct gttccgtaac cgcacaatgt ggggaatgat
     9181 gctcggattc agtggcatca actacactgc gtggctgtat ctggcctggc ttcctggtta
     9241 cctgcaaaca gcctataacc tggatttaaa aagcacaggg ttgatggcgg ctatcccttt
     9301 cctgtttggg gctgccggga tgctggtcaa cggttacgtt actgactggc tggtcaaagg
     9361 gggaatggct ccgattaaaa gccgtaagat ctgcattatt accgggatgt tctgttctgc
     9421 cgcctttacg ctgatagtac cacaagcgac aacatccatg atggcggttc tgctgattgg
     9481 catggcactg ttctgtattc actttgccgg aacatcctgc tggggcttga tccacgtcgc
     9541 agttgcttct cgcatgactg cgtcggtggg cagtatccag aactttgcca gcttcatctg
     9601 cgcctctttc gcgccgatca ttactggttt tattgttgat acaacccact cattccgtct
     9661 ggcactaatc atttgcggtt gcgtcaccgc agcgggagca ctggcgtaca tcttcctggt
     9721 tcgccagccg atcaacgacc cgcgtaaaga ttaaactccc tatgcccgat aacattttgc
     9781 ttatcgggca tttttatggc gattaaaacg tttcccagtt ctccccgcta tctgccactg
     9841 ccattttacg cggcgtagct ggcgtcacgg tttttaccac agccgatgtt tcacgctgct
     9901 gttgctgctg ttgctgaatc cggaacactg ccacagcttc ggtcaggcga ctggcctgct
     9961 cttccagcgc ggcggcggca gcggcagatt cttccaccag cgcggcgttc tgttgagtta
    10021 cccggtccat ctcagcaacc gctaagccaa cctgatcgat accacggctc tgctcatcag
    10081 aagcagaagc tatttcgccc ataatgtccg tcacgcgggt cacagcactg acaatctccg
    10141 ccattgtttc cccggcgctt tcgaccagcg tagagccaac atccactttc cccaccgagt
    10201 cttcaatcag gcttttaatt tcacgaaccg cctgggcgct acgctgggcc agattacgca
    10261 cttctcccgc aaccaccgca aaaccacgtc cttgctcacc cgcacgcgct gcttcaaccg
    10321 ccgcgttcaa agccagaata ttggtctgga aggtaatgcc gtcaattacg ctgataatat
    10381 cggcgatttt ctgcgaactg gtggagatat cgcgcatagt ctgcaccacg ttatctacca
    10441 ctttgccgcc gcgttgcgcc gtttcagaag cacttaatgc cagatggctg gcctggcgcg
    10501 cattctcggc gttctgtttc accgttgcgg tcagttgctc catgctggct gccgtctctt
    10561 ccagcgaagc ggcctgttgc tcggtgcgcg aagagagatc attattgccg gtagcgattt
    10621 cgctggcacc gctatagatg gcattggccc cgttgcgcac atcaccgacg gtacgcatca
    10681 gctctccctg catatggcgc aaactctctg ccagttgccc catctcatta gagccatcca
    10741 cctcaatcgg tttcaccaga tcgccgcctg caatatgacg aatgctgtca atcaggcgat
    10801 tcattggcgc taccagcgag gctttaatac cgaaccagac ggcgaagatg accgccagta
    10861 cgacgatcat cacgcccacc agaatccaca tcgcctggct gtaggaggca ttgttatcgc
    10921 tgacggcgat atcatagagc cgatcgtttt gctccatgta agccacatac tgcttctcga
    10981 aaccgtcctg atatccctgg gtcggctgat caaagaactc gttgattttg cctgcaccta
    11041 acagttggat cagctccgcc agcgcattgt gataaatatc gtaattacgt ttgatctctg
    11101 ccgctgcggc ggtgctctga cgcgggtcac gcggcaacgc ttcgtaatcc gcccagtttt
    11161 tttccgcctg tttcagcgaa atactggcac tctgcatcag ctcagcaacg gttgaaccgc
    11221 taccaatatt attctgatcc atcatgtagc ggatacccgc gcggttgagg gtgttacgcg
    11281 tctgcaacaa cgcgacccag ctgccattca gcgtgggttg ctgctggcga atggtttgta
    11341 aaacagtgaa attttctttg tcattcttta aggcattaaa aaacagaccg cctgatgtca
    11401 gttgtaaaag gccaaaaacg gccaaaacca gcagtaagct ggtcacgatc ttgatacgtt
    11461 ttaacatggt ttctctttcc tgtggaccga tgcaagattt tcggcctgga aagcaaaaac
    11521 tttatgaaaa ggtgtaaccc gccatccatg gaaaaaccag gcgctggtta tcagatacta
    11581 aaaagttatt cattatcgcc cgggatgtct tcttgtcggg aattgcccag agcaataaat
    11641 tcctctaaca gatgggttaa ctgttgcatt ttctcggcag taaactgagc ttcaatctgc
    11701 cgataggctt cttcaatctg tgtctgagcg cggttataca gcgcctgccc ctctttggtc
    11761 agcgagatat agagcttccg ctgatcattg atcggcttta atcgcaacac taaaccgtcg
    11821 cgttccatcc gcgtgaggat cccggtcagg cttgggcgca aaatgcaggc gcgatacgcc
    11881 agatcgtgaa aatccattga tgggctttcc gccagaatac gcacgatgcg ccactgttgc
    11941 tcagtcaaat tatgccgttt aacgattggg cgaaaataac tcatcgccgc ttctcgcgcc
    12001 tggagcaacg caatggttag tgagtcgtgc attatctttc ccctgaaaaa ttcaaaatca
    12061 ttaatataga aacaattaat catcacgtta ccgggactga gctgcccccg caattcttca
    12121 ttttatcaaa attgatgata ttttattaac ttactgatat ttaatatttt attatcttta
    12181 aatgtaaata gtttgttaat tagatcacat t
//
LOCUS       DAZSIO010000117        12118 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZSIO010000117 DAZSIO010000000
VERSION     DAZSIO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12118
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.117"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            373..2013
                     /gene="trg"
                     /locus_tag="ACHX5T_002944"
     CDS             373..2013
                     /gene="trg"
                     /locus_tag="ACHX5T_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR8468042.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2051..2974)
                     /locus_tag="ACHX5T_002945"
     CDS             complement(2051..2974)
                     /locus_tag="ACHX5T_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR8468043.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3191..4534
                     /locus_tag="ACHX5T_002946"
     CDS             3191..4534
                     /locus_tag="ACHX5T_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR8468044.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4759..6414
                     /gene="opgD"
                     /locus_tag="ACHX5T_002947"
                     /gene_synonym="mdoD"
     CDS             4759..6414
                     /gene="opgD"
                     /locus_tag="ACHX5T_002947"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR8468045.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6554..6778
                     /locus_tag="ACHX5T_002948"
     CDS             6554..6778
                     /locus_tag="ACHX5T_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR8468046.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6841..7377
                     /gene="rimL"
                     /locus_tag="ACHX5T_002949"
     CDS             6841..7377
                     /gene="rimL"
                     /locus_tag="ACHX5T_002949"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR8468047.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7372..8352)
                     /gene="ydcK"
                     /locus_tag="ACHX5T_002950"
     CDS             complement(7372..8352)
                     /gene="ydcK"
                     /locus_tag="ACHX5T_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR8468048.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8476..9468
                     /gene="tehA"
                     /locus_tag="ACHX5T_002951"
     CDS             8476..9468
                     /gene="tehA"
                     /locus_tag="ACHX5T_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR8468049.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9465..10058
                     /gene="tehB"
                     /locus_tag="ACHX5T_002952"
     CDS             9465..10058
                     /gene="tehB"
                     /locus_tag="ACHX5T_002952"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR8468050.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLTRKK"
     gene            10361..11017
                     /locus_tag="ACHX5T_002953"
     CDS             10361..11017
                     /locus_tag="ACHX5T_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFR8468051.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(11035..11541)
                     /gene="tnpA"
                     /locus_tag="ACHX5T_002954"
                     /pseudo
     CDS             complement(11035..11541)
                     /gene="tnpA"
                     /locus_tag="ACHX5T_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11549..12118
                     /locus_tag="ACHX5T_002955"
                     /pseudo
     CDS             11549..12118
                     /locus_tag="ACHX5T_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
BASE COUNT         3139 a         2835 c         3042 g         3102 t
ORIGIN      
        1 aggggctatt ccatttcatc gtccaacaaa atgggtgcag tacagttgat agaaatatcg
       61 gtcggggcct tcgtctttct gattcccggt tagcctgaaa acagaaagtc tcaggcaccc
      121 gcaggcatcc tatgaggttt ccttagggac gaaaataatc acttcacgaa attgcgtgct
      181 gttttccaga atttttcgtc attcgggtta gccagtttag ccattcgtta ctctcttcat
      241 tccaatagca ttaattttct ctgcaataat tgttgtaaaa atgtgacgca aagaggtttt
      301 tggtcataag taattaccgt caagtgccga tgactttcta tcaggagtaa acctggacga
      361 gagacaacgg taatgaatac aactccctca cagcgattag gttttttgca tcacatcagg
      421 ctggttccgt tatttgcctg cattcttggc ggtatcttag ttctattcgc attaagttct
      481 gctctggctg gctatttcct ctggcaggcc gatcgcgatc agcgtgatgt tactgcggag
      541 attgagattc ggaccgggtt agcgaacagt tcagattttt tgcgttcagc ccggatcaat
      601 atgattcagg ccggggctgc gagtcgtatt gcggaaatgg aagcaatgaa gcgaaatatt
      661 gcgcaagccg aatcggagat taaacagtcg cagcaaggtt atcgtgctta tcagaatcga
      721 ccggtgaaaa cacctgctga tgaagccttc gacactgaat taaatcaacg ctttcaggct
      781 tatatcacgg gtatgcaacc tatgttgaaa tatgccaaaa atggcatgtt tgaagcgatt
      841 atcaatcatg aaagtgagca gatccgaccg ctggataatg cttataccga tattttgaac
      901 aaagccgtta agatacgtag caccagagcc aaccaactgg cggaactggc ccatcagcgc
      961 acccgcctgg gtgggatgtt catgattggc gcgtttgtgc ttgccctggt catgacgctg
     1021 ataacattta tggtgctacg tcggatcgtc attcgtccac tgcaacatgc cgcacaacgg
     1081 attgaaaaaa tcgccagtgg cgatctgacg atgaatgatg aaccggcggg tcgtaatgaa
     1141 atcggtcgct taagtcgtca tttacagcaa atgcagcatt cactggggat gacagtaggg
     1201 actgttcgac agggcgcgga agagatttat cgtggcacca gcgaaatttc agctggcaat
     1261 gcggacctgt catctcgcac cgaagaacaa gcggcggcta tcgaacaaac tgccgccagc
     1321 atggagcaac tcactgcgac ggtgaaacag aatgcggata acgcgcatca tgccagcaaa
     1381 ctggcgcaag aggcttctat taaagccagc gatggcgggc agacggtttc cggtgtagta
     1441 aaaacgatgg gcgctatctc cacgagttcg aagaaaattt ctgagatcac cgccgtcatc
     1501 aacagtattg ctttccagac gaatattctg gcactgaatg ctgccgttga agccgcgcga
     1561 gcgggtgagc aagggcgtgg atttgccgtt gtcgccagcg aagtacggac actcgcaagc
     1621 cgcagcgctc aggcagcgaa agagattgaa ggcttgatca gtgaatcagt caggttaatt
     1681 gacctggggt cggatgaggt ggcaacggca gggaaaacca tgagcactat tgttgatgcc
     1741 gtcgcgagtg tcacacatat catgcaggaa atcgccgccg cctcggatga acaaagtaga
     1801 ggcataactc aggttagcca ggcgatttct gaaatggata aggtgacgca acagaatgct
     1861 tctctggtag aagaggcctc agcagcggcg gtgtcccttg aagaacaggc tgcacgatta
     1921 actgaggcgg tgaacgtatt ccgtctgaac aaacattctg tgtcggcaga acctcgcgga
     1981 gcgggtgaac cagttagttt cgctacggtg tgaaaatgtt caaggaggga tcgacagatc
     2041 ccttcacctt tcagaacggc attgattttc gaatagcgtt aatcatcaac tggcaaccag
     2101 aagagaacgt cgcatctacg cgggtcagta ttccaatcgg ttcgcctgca ccatgtcccg
     2161 gaacaggcag ggccaccagc gtggcatgac gcaggtcgtc ttttacagcg ccagaaggga
     2221 caaaccacac gtaatcgtat tcaacggtaa gttgacgaga tagcgaagta gacagcgttt
     2281 cgatacaacc cgaaggaatt ttacagccct ggctctgcac taatgcatct gaatgctggc
     2341 gtggcgcagt gccttctggt gatacaacga ccggccattc cagcacccgg cttagcgtta
     2401 cgttctcctg aagtagcggg tgattagggc ggacaaccag cttcaacgat tcaagaaaca
     2461 gcagttcgta attaagcccg gtcatcagtt caggatctga catccgacca atgccgatat
     2521 cgatttcccc ggtttttaaa cccgccagaa tcatagggtt actcattgtc gcaacttgca
     2581 aggtcgtctc tttttgttgc tgatgaaact gacctataac cgaaggtaat atccccagtg
     2641 ccgcagtagg tagtgcacca accctgacga catcattatt aagaccttct ttacgatgaa
     2701 gcgactgtcc ggcagtgttg atggcgtcaa gaactctgac tgcatgcgtt aaaaattgtt
     2761 cgccgggtaa ggtaagttgc gccccctgac gaccacgctc aaacaagcga gcgcccgtca
     2821 gctgctccag ttcattcaat gtattagaga gcgcaggttg actcaaatta agggtttcag
     2881 ctgcgcgccc caaagttcct tgttgtgcga cagctacgaa tgtatgaagg tggcgcaaac
     2941 ggatgcgctg actaaacaga ctattttttt ccataagcga tgttaaaaac gaagcggtgt
     3001 cgctgacaag tgaagttgtt tgattatgat aacttgattg caaaatatta ttaacaatta
     3061 aagcaattat gttacagcaa aatggataat attgatgttt tcgcggcgag atcacagttt
     3121 gtaaattctt cccgccagag tgaatgcggt tacctacact ccagattact gaccactgga
     3181 ggcagacact atggcgaaca gcatcacggc ggatgagatt cgggaacagt tttcgcaggc
     3241 aatgtcagcc atgtaccagc aagaagttcc gcaatatggc acgctgctgg aactggtagc
     3301 tgatgtgaat ctggctgtgc tggaaaacaa tcctcaactg cacgaaaaaa tggtaaatgc
     3361 agacgagctg gcgcgactga atgttgaacg tcatggggcg attcgcgttg ggactgcaca
     3421 agagcttgct actcttcggc ggatgtttgc cattatgggg atgtacccgg tgagctatta
     3481 cgatctctcg caggcagggg tgccggtaca ttcgacagca tttcggccca ttgatgatgc
     3541 ttctctggcg cgtaatccct tccgcgtttt tacatcctta ctccgccttg agcttatcga
     3601 gaacgaaatt ttgcgccaga aagcggcgga gattctgcgt cagcgcgata tcttcacccc
     3661 acgttgtcga caactgttag aggaatatga gcagcagggc ggttttaacg aaacacaggc
     3721 acaggagttt gtgcaggaag ccctggaaac gtttcgctgg caccagttag caacggtaga
     3781 tgaagaaacc tatcgcgcct tgcacaacga acatcggttg attgctgatg tggtctgttt
     3841 tcctggatgc catatcaacc acctgacgcc acgtacgctg gatattgacc gggtgcagtc
     3901 gatgatgcct gaatgcggaa ttgaacccaa aattctcatc gaggggccgc cgcgccgcga
     3961 ggtaccgatt ttactacgcc agaccagctt taaagcactg gaagagacgg tgttgtttgc
     4021 ggggcagaaa cagggcacgc ataccgcgcg ctttggtgaa attgagcagc gtggcgtggc
     4081 attaacgccg aaagggcgac aactgtatga tgatcttctg cgtaacgctg gaaccgggca
     4141 ggataatctc actcaccaaa tgcatttaca ggaaaccttc cgcacttttc ctgacagtga
     4201 gtttttaatg cgtcagcaag ggttggcatg gttccggtac cgtctgacgc cttcgggtga
     4261 ggcgcatcgt caggcgattc atcctggaga cgatccacag cccttaattg aacgtggttg
     4321 ggtagtggcg caacccatca cctatgaaga tttcttgccc gttagcgcgg cggggatctt
     4381 ccagtcaaat ctgggtaatg aaacgcagac acgcagtcac ggtaatgcca gtcgcgaagc
     4441 atttgagcag gcgttgggtt gtccggtttt ggatgagttc cagctttacc aggaagcgga
     4501 agaacgcagt aaacgtcgct gtggtttgct ttaaattctg accatccgcc tttgcaaaaa
     4561 tttgcctgat tttacaaacg aatcaggctc atcccatcga cataaaaaaa atgccgattt
     4621 atgcatattc tctcagttca acaattggat tattaataaa tattgtctag agtgagcggt
     4681 cataaataag cactttcttg ccgctgaaaa cgaccagcgc gggaccattc acaacaccag
     4741 aaggactcac tttcaggtat ggatcgtaga cgatttatta aaggttcaat ggctatggcc
     4801 gccgtgtgcg gtaccagcgg cattgcttct cttttttctc aggcagcatt cgcggcagat
     4861 tctgatattg ccgacgggca aacccagcgt tttgacttct ccattctaca gtcaatggcg
     4921 cacgacttag cgcaaacagc gtggcgtggt gcgcctcgtc cgttacctga cacgctggcg
     4981 acaatgacgc cgcaggctta taacagtatt caatacgacg ccgaaaaatc gctctggcat
     5041 aacgttgaga accgtcaact ggacgctcag ttcttccata tgggaatggg attccgtcgc
     5101 cgcgttcgta tgttttctgt agatccagca acacatctgg cgcgtgaaat tcactttcgc
     5161 ccggagttgt tcaaatacaa cgatgcaggt gttgatacca aacaattaga agggcaaagc
     5221 gatctcggct ttgccggttt tcgcgtgttt aaagcccccg aactggcgcg ccgtgatgta
     5281 gtatcatttc tcggcgcgag ttatttccgc gccgttgatg atacatatca atacggtttg
     5341 tcggcccgcg gcctggcgat cgacacttac accgacagta aagaagagtt ccccgacttt
     5401 accgccttct ggtttgatac ggtaaaaccg ggggcaacta cctttaccgt ttatgcgttg
     5461 ctcgatagcg ccagcattac tggtgcctat aagttcacta tccattgtga gaaaagtcag
     5521 gtgattatgg atgtggaaaa tcacctgtat gcgcgcaaag acattaaaca gctgggcatt
     5581 tcgccgatga ccagtatgtt cagctgcggt actaatgaac gtcggatgtg cgatacaatt
     5641 catccgcaaa ttcatgactc tgatcgtctg tccatgtggc ggggcaacgg cgagtggatt
     5701 tgccgtccgc tgaataatcc gcaaaaattg cagttcaatg cttacaccga caacaacccg
     5761 aaagggtttg gtttattgca actggatcgt gacttctccc attatcagga cattatgggc
     5821 tggtataaca aacgcccaag tctgtgggtg gaaccgcgta acaagtgggg taagggcacc
     5881 atcggcctga tggaaatccc aacaacgggc gaaacgctgg ataacattgt ctgcttctgg
     5941 cagccagaaa aagctgtaaa agcaggtgat gagtttgcat tccagtatcg tctgtactgg
     6001 agtgcgcaac cgcctgttca ttgcccatta gcgcgcgtta tggcgacgcg taccggcatg
     6061 ggcggtttcc cggaaggttg ggcgccaggt gaacactatc ccgaaaaatg ggcgcgtcgt
     6121 tttgccgtcg atttcgttgg tggtgatctg aaagctgccg cgccaaaagg cattgagccg
     6181 gtgattacgc tttccagtgg ggaagcgaag caaatcgaaa ttctctatat tgaacccatc
     6241 gatggttatc gtattcagtt tgactggtat ccgacttcgg actccactga tccggtcgat
     6301 atgcggatgt atctacgttg tcagggggac gctatcagtg aaacatggct gtatcagtat
     6361 ttcccgccag cgccggataa acgtcagtat gttgacgacc gcgtgatgag ttaatcgttt
     6421 tttcttcggc accttcttcg ggaggtgccg tctggttaaa cacgatcccg ctcgcatttt
     6481 tccctaagtt aaatgagtaa tctgatggtg tgtatttcag atacaccgtg tcagccacta
     6541 acagggagtg cgtatgtttc cagaataccg agatttaata tcccgtctga aaaacgaaaa
     6601 tcctcgcttt atgtccttgt tcgataaaca caataaactt gatcatgaaa ttgccagaaa
     6661 ggaaggttcc gacggtcgag ggtacaatgc ggaagtggtc cgcatgaaaa aacaaaagct
     6721 acagttaaaa gatgagatgc tcaaaatcct gcagcaggag agcgtcaaag aggtgtaaac
     6781 tttcctaagc cgccagccag gcggcttttt taacaacagc atggattgac tggagataag
     6841 atgactgaaa cgataaaagt aagcgaatca cttgaattac atgctgttgc ggaaagtcac
     6901 gtcacacctc tttatcagtt aatctgtaaa aataaaacct ggttacagca gtcgctaaac
     6961 tggccgcagt tcgttcagag tgaagaggac acgcgaaaaa cggtgcaggg taatgtgatg
     7021 ttgcatcaac gcggctatgc caaaatgttc atgattttca aagaagatga acttatcggc
     7081 gttatctcgt ttaatcgtat tgaaccactg aataaaactg ctgaaatcgg ctactggctg
     7141 gacgaatctc atcaggggca ggggatcatt tctcaggcgc tgcagacatt gattcatcat
     7201 tacgctcagt ctggtgaact tagacgcttc gtgatcaaat gtcgggtgga caatccgcaa
     7261 agcaaccagg tcgctttgcg caatggtttt atccttgaag gttgcctgaa acaggctgag
     7321 ttcctgaatg atgcctatga tgatgtgaac ttatacgcgc gtattatcga ttcataataa
     7381 ccctgccagc ggcgttcgcg taatgcgctc ctcgccgtta atgacttttg gaccacggac
     7441 atgaaccgtg tcgccatcaa aggcttcaac gacggcgtgg tcggttaact ccacatggtt
     7501 ttcaattatc accgcacctg taatacggct ctcaccttgt attacaacgt gctcgtcgag
     7561 cagaatcggt ccaccacgta ccacggcatt tccgccaatc aagacatgat gttttaacac
     7621 acaattacct tccacaatgg catattccgc cacctgcgaa ctgtaatgaa tcgtcggaat
     7681 ggcatcttct tctatgccag ctttcacctg cgcgtgaccg tagactttag cgcaatcgca
     7741 tagccagacg ttgttctctt cattaccttc aacactggca aaatcaaaaa cttcggcgcg
     7801 atgttcaata aaagcatacc ggacgacggc atcgccataa atttgtgcct ggtggacaat
     7861 acgcgaggcg ctaacccttg cgcgatcata aatttggagc aggagttgat ggtcgggcgt
     7921 taagccttgt gcggcgacga tcatagaatg ttgatcaatt aaggcgtgac caaatattct
     7981 acattgcacg taaactaatg agtcatgaat ggtgacactg tcacttatat atgcaccctg
     8041 gctgatttca ctattatcga tccagacgtt atccgttgcg taaacctctc cccacagcac
     8101 gctggtgccg gtgatacgcg tattaccgga aatcaccgtt ccgccgaacg caatagcatt
     8161 ctggtcgtaa atccagcaat ttccttcctg tgctaacacc gtttcgcgat cgatccagcc
     8221 gccagcggtt cctgctatca catcgttaaa atcgctaatg gcgataatct gccgtaataa
     8281 cacacttttt ttagtgccat cttcctgata actaaaggct cgctgttctt cacttaggcg
     8341 atatttgcgc atagcgtttt cccacaggtg acttactata accgtagcaa attctgcggc
     8401 tctggctatg ctcgagaaaa ttccataaaa tgcatttcaa atatacttta taaattaaac
     8461 aaaatgagta agaagatgca gagcgataaa gtgctcaatt tgccggcagg ctactttggt
     8521 attgtgttgg ggacgatagg gatgggattt gcctggcgct atgccagcca ggtttggcag
     8581 gtcagccact ggttagggga tgggctggtg attctggcga tgatcatctg gggattattg
     8641 actagcgcat ttattacccg actcatacgc tttccgcata gcgtgctggc ggaagttcgc
     8701 catccagtgc tgagcagttt tgtgagtttg tttccggcaa cgacgatgct ggtggcgatt
     8761 ggttttgttc cgtggtttcg cccactggcg gtgtgcatgt tcagttttgg tgtcgtggtt
     8821 cagttggctt atgccgcctg gcaaactgcg ggattatggc gcggatctca ccctgaagaa
     8881 gctaccacgc ctggactgta tctgccgaca gttgccaaca actttatcag cgcaatggcc
     8941 tgtggtgcgt tgggctacac cgacgccggt ctggtgtttt taggcgcagg cgttttctca
     9001 tggctaagcc ttgaaccggt gatcttgcag cgtctgcgca gttcgggaga attacccacg
     9061 gcactgcgga catcactcgg cattcagctc gctcctgcgc tggtggcctg tagtgcctgg
     9121 ctgagcgtca acggcggcga gggtgacacg ctggcgaaaa tgcttttcgg ttatggactg
     9181 ctgcaactgc tgtttatgct acgtctgatg ccatggtatc tctcccagcc atttaatgct
     9241 tcattctgga gtttctcgtt cggcgtatct gcactggcaa ccaccggttt gcatctgggg
     9301 agtggcagcg ataatggatt tttccatacg ctggcggtgc cgctgtttat ctttaccaat
     9361 tttattattg caatactgct catccgtact tttgcgcttc tgatgcaggg aaaattgtta
     9421 gtcagaaccg agcgcgccgt tttaatgaaa gcagaggaca aagaatgatc attcgtgacg
     9481 aaaactattt tactgataaa tatgaattaa cccgcacaca ctctgaagta ctggaagcgg
     9541 tgaaagtggt taaaccgggt aaaacgctgg atctgggctg tggcaatggt cgtaacagtc
     9601 tttacctggc agccaatggt tatgatgttg acgcatggga taaaaatgcc atgagtatcg
     9661 ccaacgtcga gcgcattaaa tccattgaaa atctggataa tttacacacc cgggtcgttg
     9721 atctgaataa cctcacattt gatggacagt acgattttat tctttcgact gtggtgctga
     9781 tgttccttga ggctaaaacc atcccagggt tgattgccaa tatgcaacgt tgcactaaac
     9841 ctggtggtta caacctgatt gtggcggcga tggataccgc tgattatcca tgtaccgtcg
     9901 gcttcacgtt tgccttcaaa gagggagaat tacgtcgata ttacgaaggc tgggagaggg
     9961 tgaaatacaa tgaagacgtc ggcgagctgc accgcaccga cgccaacggt aatcgtatta
    10021 aactgcgttt cgccacgatg ctgacacgta aaaaatgacc cggtaagcac aaaacgcgtg
    10081 aaaattcccc acgctgagat gatttactgt tcttcttttc ggtaagcata ttttttatcg
    10141 aagggatgtg aaattaatca cagtagtcga agttttttag cagcttaact tactgaaatt
    10201 taactactga tgattgactt agcccctttt tcggcattga ctatgtcgtc tgaaaagggg
    10261 ctgaaaaatt tattttcacc aacacttttt ttgccacaac acgaagcggc gctttttgct
    10321 ataacttaga aagtaatata atcatctcag gaaactattc atgcgtacca catcatttgc
    10381 gaaagttgca gctttatgcg gcttgttggc tctgtctggt tgtgcctcta aaatcaccca
    10441 gccagataaa tataacaatt actctgattt aaaagaaaca acctcagcaa caggtaaacc
    10501 tgttttacgt tgggtagacc cgagttttga tcaaagcaaa tatgacagca tcgtctggaa
    10561 cccaatcact tattatccgg taccaaaacc ttctacccag gtagggcaga aagttctgga
    10621 taaaattttg aactatacca acaccgaaat gaaagaagcg atagcgcagc gtaaaccagt
    10681 ggttaccacc gctgggccgc gtagtctgat tttccgtggg gccattaccg gtgtagatac
    10741 cagcaaagaa gggctgcaat tctatgaagt ggttcctgtt gcattagtgg ttgcggggac
    10801 gcaaatggct acaggccacc gtaccatgga tactcgcctc tattttgaag gtgagctgat
    10861 tgatgcagcg actaataaac cggttatcaa agtcgttcgt cagggcgaag gtaaagacct
    10921 gaataacgaa agtacgccaa tggctttcga aaatattaaa caagttattg atgacatggc
    10981 gaccgatgcc accatgtttg acgttaacaa aaagtagttc cagacgcgcc atcgttagat
    11041 ggcgcttttt atccggtgcg ccgtaaaacc ccatccttca gggcggggat ataaggcgcg
    11101 gttttccacc tgaccaggtg tttgctgttg ttcaatgtat tggcggatga cggatattgg
    11161 cgcacctccg cagctactgg caaaatagcc aggactccac aaaacgcctt tgtagtaata
    11221 cctgactgca atatctggtc gatctcgtcg cagtaaccta cccgatacgc ctttgaggct
    11281 gtttaccaga ctggatatcg ccagtttggg aggttagtta attaacaaat ggacgtgatc
    11341 tggttcgcca tccatttcaa ccagttcagc ttcaaaataa gcacatacat ttgaaaagta
    11401 agtgcgtagt ttttctgtcg cgtcatggtc aaaaatctgg cgtcggtatc tggtgacaaa
    11461 gactaggtga acatgcatca ggaaaacaca atgccttcca cgccggatat cggtttcttt
    11521 tttcacagac caaagtatca tttgacctgt gaaacgatta caggcattta aattccagtt
    11581 aagaccaggt ggtcaacagg agtgtgaaat gaggcgcttc gcaggcgcat gtcgtttcgt
    11641 tttcaatcgt gctctggcac gtcagaatga gaatcataag gccggtaata aatacatccc
    11701 ttacgggaaa atggcttcct ggctggttga gtggaaaaat gccactgaaa cgcaatggct
    11761 taaagattct ccctcacagc cattgcaaca gtcactgaaa gaccttgagc gggcttacaa
    11821 aaacttcttc cggaagcggg ctgcttttcc ccgattcaaa aagcggtgac agaatgatgc
    11881 attccgctac ccgcagggcg ttaagctcga tcaggaaaac agccgtattt ttctgccgaa
    11941 actgggctgg atgcgctacc ggaatagccg tcaggtcacg ggtgttgtga aaaatgtcac
    12001 tgtcagccag tcctgcggta cgtggtacat cagtattcag acagaaagtg aagtatccac
    12061 tccggctcac ccttcagcat caatggtcgg gctggatgct ggcgtggcta aactcgcc
//
LOCUS       DAZSIO010000118        12113 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZSIO010000118 DAZSIO010000000
VERSION     DAZSIO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.118"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..370
                     /locus_tag="ACHX5T_002956"
     CDS             <1..370
                     /locus_tag="ACHX5T_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468052.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="ACHX5T_002957"
     CDS             complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="ACHX5T_002957"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR8468053.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="ACHX5T_002958"
     CDS             complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="ACHX5T_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR8468054.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="ACHX5T_002959"
     CDS             complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="ACHX5T_002959"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR8468055.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="ACHX5T_002960"
     CDS             complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="ACHX5T_002960"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR8468056.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3448..5049)
                     /gene="tap"
                     /locus_tag="ACHX5T_002961"
     CDS             complement(3448..5049)
                     /gene="tap"
                     /locus_tag="ACHX5T_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR8468057.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5095..6756)
                     /gene="tar"
                     /locus_tag="ACHX5T_002962"
     CDS             complement(5095..6756)
                     /gene="tar"
                     /locus_tag="ACHX5T_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFR8468058.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6901..7404)
                     /gene="cheW"
                     /locus_tag="ACHX5T_002963"
     CDS             complement(6901..7404)
                     /gene="cheW"
                     /locus_tag="ACHX5T_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR8468059.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7425..9389)
                     /gene="cheA"
                     /locus_tag="ACHX5T_002964"
     CDS             complement(7425..9389)
                     /gene="cheA"
                     /locus_tag="ACHX5T_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR8468060.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9394..10320)
                     /gene="motB"
                     /locus_tag="ACHX5T_002965"
     CDS             complement(9394..10320)
                     /gene="motB"
                     /locus_tag="ACHX5T_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR8468061.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10317..11204)
                     /gene="motA"
                     /locus_tag="ACHX5T_002966"
     CDS             complement(10317..11204)
                     /gene="motA"
                     /locus_tag="ACHX5T_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR8468062.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11331..11909)
                     /gene="flhC"
                     /locus_tag="ACHX5T_002967"
     CDS             complement(11331..11909)
                     /gene="flhC"
                     /locus_tag="ACHX5T_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR8468063.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11912..>12113)
                     /locus_tag="ACHX5T_002968"
     CDS             complement(11912..>12113)
                     /locus_tag="ACHX5T_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR8468064.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2784 a         3388 c         2933 g         3008 t
ORIGIN      
        1 cactacgcta atgagttggg atgccgtgct ctatgatcta cgtagtgaaa aaattatcag
       61 taaagataat tacttgaccg acttaaaccg cagacatctt gaacttgttc ttcatactaa
      121 ccccaatcca agcggttcgg taacgaagat tttacgcact atttttacta agcaggtaaa
      181 gagtttaggg ccggaactct gtcatttctt gcaccatgaa ctgacgaaaa aacgttatga
      241 agtatggcat cactacgatg tactgcacta ccagtcatcg tcattttctc gttcacaatt
      301 taatgcattg ataaatttac tggcgcaaca atcacgcttc gctgatctaa aattatcaaa
      361 ctacaaataa ccatcctcca cgtactccct gaacgatacc cgccaccctg ctatcggctt
      421 ggcatcatcg cgcaggccga agttcaggca atacaaatca aaatccaaga ctatccaaca
      481 aatcgtccac ctgatcctga ctggctacca cgccggcttt gctggtatcg acctgaggtc
      541 cattaagcaa actctggttt tcacgttttg gacgcgactc ctgttccggg atgttttcca
      601 acagcaccat cagcaactgg cgttcgatct cctgaatgac atccatcatc cgcttaataa
      661 cctgcccggt gagatcctga aaatcctgcg ccatcatgat ttccagcagt tgcgcgttag
      721 taaagctggt atgcgcgggt acatctgcca gaaattgtcg tgtatctgtt accagttcac
      781 gggcgtcggc aaggtcaatc ggatcggcaa accagtcatc ccaacgttgg gttaacgctt
      841 ttgctgattt ctccatttga tcctgatgcg gttgtgacgc ctcaacactg ttcagcgccc
      901 gctccgcagc ctgggcggtc atctgcacaa catagtacaa acgatcgcgc gcatcgggga
      961 tggcttccgc cgcttcggca atggcctgaa ccagccccag ttcccgcaaa ctgtcgcgca
     1021 gcatatgcgt caggctgccg atgcgcgcaa tgatatcgcc agctgaatgc tcgtcagcag
     1081 gtttgattga tggttgcatc atagtcgcat cctcacatgc ccagtttctc aaagattttg
     1141 ttgagttttt cctccagcgt cgcggcggta aatggcttca ccacatagcc actggccccc
     1201 gcttgcgccg cagcaatgat gttctctttc ttcgcttctg cagtcaccat taacactggc
     1261 aatgccgaca tcgcgccatc cgcacgaatt gttttcagca attccaggcc atccatattg
     1321 ggcatgttcc agtcggagat aacaaatcca taaccgcctg cctgcaactt attgagagcg
     1381 tcgacaccat cttccgcttc ctcaacatta ttgaatccca gctctttcag caggttacgc
     1441 actatgcgtc gcatggtgga aaagtcatcc acaaccaaaa atttaagttc tttatccgcc
     1501 atttcacact cctgatttaa atacgtatcg cctgtccggc actaattgtt gccaacattt
     1561 gctggcttac ctggctaaga tcgaccactt cgcagacacc acccatattg atggcctcgc
     1621 gcggcatgcc gaacaccacg caacttgctt cgttttgcgc aagggtccat gcccccgcct
     1681 gacgcatcgc caacattccc gccgcgccgt cgttgcccat accggtcagg atcaccccaa
     1741 ccgcattacg ccccgcctgt ttggcgacag aatggaacaa cacatctacc gaaggccgat
     1801 gacggttaac cgccgggccg tcgtgaattt tgatttgata atttgcgcca ctacgcgcca
     1861 gttccatatg ccgatcgccc ggcgcaatat aggcatgccc cggcaagaca cgttctccgt
     1921 cttcggcttc tttaaccccg atctggcaaa gcttattaag tctgtcggca aaagagcggg
     1981 tgaaaccggg cggcatatgt tgggtaatta acaatgccgg gctggaaagc ggcaacggtt
     2041 gcagtacgtg acgaattgcc tcagttccac ccgttgaagc accaatcgca atcagttttt
     2101 cagaactcaa caacggccct gccttcagcg ttgtcggtgc tgacaatggc ttatgtgctg
     2161 caaggctcgc ctttgctgcc gtacgcacct tttcagcaat catttcgcta tacgccagca
     2221 ttccttcgcg aatacccagt tgcggtttgg tgacaaaatc tattgccccc agctccagcg
     2281 cgcgcagcgt gacttctgat cctttgccgg tcaaggaaga aaccatcaca acgggcattg
     2341 gacgcaaacg cattaatttt tcgaggaaat ccagtccgtc catccgcggc atttcaacat
     2401 ccagcgtcag cacatcggga ttgaatttct taatcaagtc acgcgcaacc agcggatcag
     2461 gcgcggtcgc caccatttcc atgtcgctat ggctgttgat gatttctgtc atgatctggc
     2521 gcatcagtgc cgaatcatcg acagataaca ccctgatttt gctcatcgtt aatccttact
     2581 tagcgcatac accgtctgac cacgcagcgt gaagcggcgc tcgaggtggc taaagttttc
     2641 agagtgaccc gcaaacagca atccgtcggg tttaaggagc ggaacaaagc ggcgcaaaat
     2701 ctcctgctgg gtagtttgat cgaagtagat catgacgtta cgacagaaga tcgcatcaaa
     2761 cggccccggc acggtgtact gtttcgccag taaattcagc ggggcaaaat caacatagtt
     2821 cgccagctcc tgacgcacgc gtaccagccc ttcatgcggt cccgtccctc gcatgaaata
     2881 ccgttgcagt tgctgcggcg tcaggttttt caactcttca tggcgataga taccgcttct
     2941 ggctttttcc agcacttcgg tgtcgatatc actggcaaac actttccagc gtccgggcac
     3001 ggtgcccaat gtgtcagcca gagtcatcgc aatgctgtac ggctcttcgc cggtcgaagc
     3061 cgccgcgctc catacgcgat cctcgccaga acggcgacgt gcgtgatccg cgagcagagg
     3121 gaaatgatga gcctcacgga aaaatgccgt cagattcgtg gtcagcgaat tgataaacgc
     3181 ctgccactca ccgctgtgct gattagattc cagcaagttc agataatgac cgaaatccgt
     3241 cagtcccaga gaacgcaaac gacgaaccag tcggttgtaa accatgtcgc gtttatggtc
     3301 agccagaacg atcccggctc gttgatagat caattgactt atccgccgaa aatgcgcgtc
     3361 ggaaagcgcc aggcgctcgg tcatctgtaa caataaagac gtttgcccac agggcagaga
     3421 tgaagtcata gcgccttctc aatcacttca ggataccact ggcgcaattt gtaactgcgc
     3481 cgactcatgt cgtgctactt catgttcttc aagggtaaat accgccacac gcgacgaaag
     3541 atggtcggcc tgattcgcca gttgttccgt tgccaccgcc gcttcttcta ccagcgagga
     3601 gttctgctga gtcacctgat ccatctggct gacagcctgt gcaacctgct caatcccccg
     3661 ccgttgttct tccgacgccg aagcaatttc tcccataatg tcgttcacgc gggtcaccga
     3721 actgacaata tcggtcatgg tcgcggcggc gttattcacc agtttcgaac cctgctggac
     3781 acgattgact gactcttcga tcagcccttt gatctctttt gctgcctgcg cgctacggct
     3841 ggcaagattg cgcacttcac ctgccactac cgcaaaacca cgcccctgct ctccggcgcg
     3901 agccgcttcc actgccgcat tcagggccag aatattggtc tggaaagcaa ttccgtcgat
     3961 aacgctgata atgtcgccaa ttttttgcga gctggtggcg atctcctgca tggtgtgagt
     4021 catggtactg acctgaacac cgcccgcctg cgccgttgtc gcggcatttt ttgccagttc
     4081 cgacgcctgt cgtgcgttat cggcgttttg ccctaccgtg gcggttaatt gctccatact
     4141 ggcggccgtt tgtgccagcg atgccgcctg ctgttcggta cgacttgaga gatcgttatt
     4201 gcctgcgaca atctccgcga tcccaatgtg catctcatgg cttcccttac gcacatcact
     4261 caccgtccca cgcaaagcct gctgcatggt cttcaggctg gcaaaaatgg cggtgatctc
     4321 attacgacca tataccgcaa tcggacgcgc cagattaccc gcagcaatgc tgtcaaaatg
     4381 gctaccgata atggccagtg gttgaacaat catcttgcgc gtccaccaca gcgcactgct
     4441 gatgtagatc gccgcaacaa taatcatgct gataaacacc agcgccgaaa tctgatagtt
     4501 acgctggctt tgcgcactgg cggcttccag cacatggttg atctccagct gccaggcctc
     4561 aaagttaacg tcaaacgcat tctgtgatcc ctgcaccggc gcagtgagga aatccgaaag
     4621 ttgattactt tcaagccagg tcgcctgatg ttcgagatcg ttgtgccagc gggcaaaact
     4681 tttctccgtc tctttttgca atgccctgac gtgctcgttg cccgcagtca tcgccataaa
     4741 acttttaaac agcgtggtgg attgcgtcag actggcgcgc gccgtcgtca tcaacgtttt
     4801 aatgtcatcc gccggatagc taagcgctgt cagagtgcct gctttgttca gcgcggtact
     4861 ggcctgtaac attaccgccc gagtttgcgc taatgccgca cgttgctgat tgctctgctc
     4921 gccgtgattc agtcgttgca aatcgtcgcg aaatgcccaa aaagacatgc cgttactgcc
     4981 aatctgcaag atcccgcaga gaatcaaaat taaaaacagc gtggtcgaaa ttcgaatacg
     5041 attaaacatc aacgctcccc atcaggcggc aatgaccgcg ttagtaaaaa ctcgtcaaaa
     5101 tgtttcccag tttggatctt gttcagtaat tcgcagtcgt ggctgtgccg gtggttgctc
     5161 actggcagga cgggatggtg tttgcggttt attggtgagt gggctggctg ccagacggaa
     5221 cgcggaaacc gcttgcgtta aacgactcgc ctgttcttcc agcgcagctg cggcggcagc
     5281 tgattcctgc accagcgatg cgttctgttg cgtgacgcga tccatttccg aaaccgccaa
     5341 tgcgacttga tcgatgccac ggctctgttc atccgatgcc gatgcaatct cgcccataat
     5401 gtcagtcacg cgagtgacag cattgacgat attgttcatt gtttccccgg cgctttcgac
     5461 cagcaccgaa ccggtatcaa tgcgcgagac ggagtcttca atgagggctt tgatctcttt
     5521 tgccgccagg gcgctgcgac tggcaagatt acgcacttca cccgccacca cggcaaaacc
     5581 acggccctgt tcacccgcac gcgcggcttc aaccgcggca ttcagcgcga ggatattagt
     5641 ctggaaggca ataccgtcga taacgctgat aatgtcggca attttcttcg aactatcggc
     5701 gatctcatgc atcgttttca ctacgccatc caccactttg ccgccgtgct gggcggtgtc
     5761 ggaggcactt tgcgccagtt gcgaggcctg gcgggcgtta tcggcgtttt gcttcactgt
     5821 cgcggtgagc tgctccatgc tggcggcagt ttcttccagc gcggatgcct gctgttcagt
     5881 acgggaggaa agatcggtgt tgcccgccgc aatttcacgg gtaccggcat agatggcatc
     5941 tgaaccttcg cggacatgag tgacggtgtc agtcaaagag cgttgcatat gtgaaacgct
     6001 ctgcgccagg tcacccattt cactgcgccc gtcaatggtc agggtattcg ccaggttacc
     6061 accggcgatt tcgcaaatgt gagcaataat ttttgccagc ggagtaagca acatacggcg
     6121 aatgccgtac cacgccacca gcagaatcaa taccaccacc agcgcgataa ccgccagttg
     6181 ccactgggca aatcggtaat catctgcgtt gtcagtgacg atatcgcgat acagtttttc
     6241 actgctgagg gcgtactgag caaacgcttc gcccattgca ttttgcattc cctgggttgg
     6301 ctgagcgaaa taagctccag tattgccata atcaagataa tcaatcagtt cagttaacgc
     6361 tgtgtaatag tttttatatt tttcatcaaa attacggctg gtagcgacca tttcaggtaa
     6421 cggtgccatg cttttgaatt ttttataatg cgtcgcggcc tgggccaatg ttttcctggc
     6481 gctatcgagc aattcaactt tggcgttact ttgttgattg gaggaatcca tcatcatccg
     6541 caccgctgaa cgactcaggt taatgcgcgt ttgcagcatt aaatcccagg ttgacgtcag
     6601 ctcgccctgc tgttcccgta attgattgga aaccacaaag ctcttctggc tatggtgaag
     6661 ggaagaaaaa aacaaactgc cggaaataag ctgtaacagt gcgaataccc ccagcaccat
     6721 taccaacagc gtgactacgc ggatacggtt aatcataagg caccttcctg aaaacaagtt
     6781 gatctcgtta tcggcaagaa ggggggaaac tttattgctg atgccacccg ccgcgaaatt
     6841 gaaataaaaa acccgatgcg cagatcatcg ggttcatttc aattgaggaa atcgggagaa
     6901 ttacgccact tctgacgccg cgctatctaa cagcgccatc tcttcgctgt tcagcagttt
     6961 ttcgatgttc accagaatca acatacggtc gcccagtgcg cccagtccag tgagatattc
     7021 tgttgaaagc gtcacggcaa actctggtgc cggacgaatt tgctccgccg tcaatgaaag
     7081 cacgtctgaa acgccgtcaa ccacgatgcc gaccacccgc tgtccgagat tcaggacgat
     7141 aactaccgtg ttgtcgttat agtccacatc cacctggctg aacttaattc gtaagtcaac
     7201 aatcggcaca ataacgccgc gcagattcgt gacgcctttg ataaacgctg gcgtgttcgc
     7261 aatccgtgtt acctgatcgt agccacggat ctcctgcact ttcaggatat caataccgta
     7321 ctcttcatca ccaagggtaa ataccagaaa ttcctggcct gacggctcgc tggccagctt
     7381 tgttacattc gtcataccgg tcatattgtt acctttttac tcattcaggc ggcggtgttc
     7441 gccatacgtt gttcgcggtt tatcgcctgc aaggcggaga catcaacaat cagtgccacg
     7501 ctgccgtcgc caagaatggt cgcagcagaa atgccgggga ctttgcgata gttactttca
     7561 aggtttttaa ccacaacctg gtgttgacca attaattgat ccaccagcaa ggcgtagcgg
     7621 cgaccgccac tttgtaagat caccacaatt ccctgggtgg cttcggtttt cgcgcccgcg
     7681 acgttgaaca ctttccacag ttcgacgatg ggcagatatt caccccgcac ttccagcacc
     7741 cgctcgccgc cggccagtgg atggagatcg gcttcacggg gttgcagtga ttccataaca
     7801 gcattcagcg gcagaatgaa aacttcatcc gcaacgcgta cggacatgcc gtcgaggatg
     7861 gccagcgtca gcggcagtaa aatgcggatc gtagtgccag taccctgctt cgactggatt
     7921 tcgacatgac cgcccatctc ctggatatta cgtttaacga cgtccatgcc gacgccgcgc
     7981 ccggagacgt cggtgacctg ctctgccgtg gagaagcctg gtgcaaatat cagcattgca
     8041 acttcgtcgt cgctcatgtt ttcgctgaca gtcaaacctt gcgaggccgc ttttgccaga
     8101 attcgctcac ggtttagccc cgccccatcg tcggtcactt caatgcaaat gttgccgccc
     8161 tgatgttcgg cagacagaat taaatttccg acgctgtttt tacctgcggc gagccgtttt
     8221 tctggcagtt caataccgtg atcgaggcta ttgcgtacca ggtgggtcag cgggtcgata
     8281 atgcgttcta tcaggctctt gtcgagctcg gtggaactgc ccaccagcgt cagttctacc
     8341 tgcttgccga gttttcccgc cagatcacgc accagccggg gatagcgact aaagacatat
     8401 tccatcggca tcatgcgaat cgacatcact gattcctgca aatcacgggc gttacgttgt
     8461 aactgcccca tgctggtaat caaatcacca tgattaaccg ggtccagttc gctggaacgc
     8521 tgggcaagca tggactgggt gataaccagc tcgccgacga ggttaattaa ttgatcaacc
     8581 ttttctaccg ctacacggat gctggtggat tcactgctgc gcgtcgtttt ttcccgctcc
     8641 acgcggccgg ttggcgcttg ttcggctgcc agtttaagca caggtggggt agctattttt
     8701 ggcgaaactt ctactgtttc aaaggtaatc tgatcggctt caatcacaaa acagagtacc
     8761 gctgtgatgt catcttcggc gatatcgccc ggtaatattg ccgagagaga atccgcccct
     8821 ttcaccacgt catttaacgt tgtcagatgc cccagctctt cttccagcag gtcgacttcg
     8881 ccggccttca ggcgcgaaag gataattcgt cgcggcaact gactgcgact ttgctcatct
     8941 tgcggttcac ttttggcaac cacacttaat cgggtcactg cggatggcgt ttcgcctttc
     9001 gcttctaatg ccagttgacg caaggcctgg cagatataat cgtagctggc ggcatccggc
     9061 tcttgcgact gtttataagc gtcgagctgt tcttgcatga tatccttcgt ttccaaaaac
     9121 agattgataa tgtcggtgtt gagttgcatc tcacctcgtc tggcttcatc gagcaggttt
     9181 tccatcagat gcgtggtttc ctgcaaaacg ctgaagccaa aagttcctgc ccctcctttg
     9241 atcgagtggg cagcccgaaa gatggcattc aattgttcgg catctggcgc ttccggctgc
     9301 aaaaccagca gatgctgctc catatcagcc aacagttcgt ccgcttcatc aaaaaatgtc
     9361 tgataaaaat cgcttatatc gatgctcacg ctgtcacctc ggttcggctg atggcattgt
     9421 gggaacactg acctgtggtg caacctcagg tttttctagg gcgcttactg gctcattctg
     9481 gctttcggcg ttttcatgca aaatggcctg ttcggcttgt ttgttcagta ccaacaggct
     9541 gatgcgacgg ttgacggcat catcaggtcc gcgatcgctt aagcgcatcg ttgccgccat
     9601 gccgacgaca cgtaacactt tgccgctatc caaccctccg accatcagtt cgcggcggga
     9661 tgcattggcc cgatcggcag aaagctccca gttgctgtaa cctttctcac cgctggcgta
     9721 gggaaaatca tcggtatgac ctgaaaggct aatacggttg ggaataccgt tcagcacagg
     9781 cgcgatggcg cgcagaatgt cgcgcatata gggttcgaca tcggcactgc cggttctaaa
     9841 catcgggcga ttctggctat cgatgatctg aatacgtaga ccttcctgga ccagatcgat
     9901 tttgagatgg agacgtaacg cccgcagttt cggatcggac tctatcaact ggtcgagatc
     9961 accccgcaat ttccgcaatc gactttgctc catgcgtttt ttcagctctt cgatgttcgg
    10021 ctgcttattc acttccccct ggctttgggt gtaatcatca ccaccgccgg gaattgggct
    10081 ttcactattg gaaatgcgat cgccgcccgt aaccgctgtc gccagtggag tccggaagta
    10141 ctccgcaatc tgaatcaact cttttgggct ggagatggag atcagccaca tcaccagaaa
    10201 aaaggccatc atcgcagtca taaagtcggc ataagcaatc ttccacgatc catgtgctgc
    10261 cccgtggctt ttggctttgc gtcgtttgac gacaataatc ggatgcgctt gattcttcat
    10321 gcttcctcgg ttgtcgtctg ttgttgcgga tttttcaccg cacgcacatg ctcttccagt
    10381 tcaataaacg acggacgttc gctggaatag agcgttttgc gaccaaactc aacggcgata
    10441 ggcggtgcgt aaccgttcag attagaaagc agagtgactt tgacgcactg catcattttg
    10501 ctggtttcgg cgcttttctg acgtaaaaca ctcgctagtg gggaaataaa tccgtaagcc
    10561 aataaaatac cgaggaaagt ccccaccatc gcatgtgcga taagcgcccc cagctcggcg
    10621 gcaggacgat cggctgaacc taacgcgtga acgcccccca ttacagccgc gacaatacca
    10681 aacgccggaa gtgagtcccc gaccagcgcc agactgttcg ccgggacttc tgcctcgctt
    10741 tcgtgcgtct caatctcttc atccatcagg gcttcgattt cgaaggtgtt catgtgaccg
    10801 ctgataatca ggcgcagata atcgacgata aaatcaagca tgacgctatc cgcgaggatg
    10861 cgtgggtagc tggcgaagat ctcgctctca cggggatttt caatatcacg ttccagcgaa
    10921 aacatcccca tctgccgcga tttcgccatc aaccgataaa gcagagccag cagatccata
    10981 tacattgctt tggtgtattt ggagcgacga aacagcaacg gcagcgcttt cagcgtgcct
    11041 ttaatcgctt tgccattatt gccgacgata aacgacccaa tccctgcacc ggcaataatc
    11101 accagttcag cgggttgata gagtgctcca aggcttccac cggtcatcaa ataaccgccg
    11161 aaaactgtac cgagaacaac caggtaacct aataagataa gcacgacatc atccttccac
    11221 tgttgaccat gacaggatgt tcagtcgtca ggcgttaacg cgcgattggg gaaaaaaaaa
    11281 gcagcggtac gtcgttaccg ctgctggaat gttgcgccac accgtatcag ttaaacagcc
    11341 tgtactctct gttcatccag cagttgtggg ataatatcgg caggattctg ggaaagttta
    11401 cgtcttttta ctgcccggga tggcggttga cataagctgc aggcaaagct gccaacaggc
    11461 tggtgagcgt gggtaataaa attgccgccg cagcagttgc agctggaaag ttgcagtaat
    11521 ccactttcaa caaaccgcac caatgtccag gcacgggtta atgccagcag tggtccttct
    11581 tctgcttgtg ggcactgttc aaggtataaa cggtaggctt tgatcaccgc atcgacgcca
    11641 ttacacaaac cggttttcag taaaaactgc catgcattac agaacatcga agcatgaacg
    11701 ttttgttccc aggtcataaa ccagtcggtt gagaatggca gcatgccttt cggcggtggg
    11761 cttccgcgca gttctttata aagttttatc aggcgtccgc gacttaactg tgtttcgctt
    11821 tccagcatct gcaaacgagc gcccagggtg atcaattcca ttgccagctg aatatcccgc
    11881 gcttcctgaa caatgctttt ttcactcatg atcaggccct tttcttgcgc agcgcttctt
    11941 caggctgatt aacatcattc agcaagcgtg ttgagagcat gatgccggta tgaatttgct
    12001 ggagatcgtc aacgcgggaa tcttgcgtca actgagtaat cgtctggtgg ctgtcaaaac
    12061 ggaagtgaca aaccagctga ttggtttctg ccagcttaac catttgcgga aga
//
LOCUS       DAZSIO010000119        12024 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZSIO010000119 DAZSIO010000000
VERSION     DAZSIO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.119"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            3..866
                     /locus_tag="ACHX5T_002969"
     CDS             3..866
                     /locus_tag="ACHX5T_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR8468065.1"
                     /translation="MSIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELRDL
                     LIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNTLE
                     LTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSLLD
                     FLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKTRE
                     ATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHLRV
                     QSTSKTAPQAK"
     gene            complement(1069..1422)
                     /locus_tag="ACHX5T_002970"
     CDS             complement(1069..1422)
                     /locus_tag="ACHX5T_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR8468066.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1560..3206)
                     /gene="groL"
                     /locus_tag="ACHX5T_002971"
     CDS             complement(1560..3206)
                     /gene="groL"
                     /locus_tag="ACHX5T_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR8468067.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3250..3543)
                     /locus_tag="ACHX5T_002972"
     CDS             complement(3250..3543)
                     /locus_tag="ACHX5T_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR8468068.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3819..5075
                     /gene="yjeH"
                     /locus_tag="ACHX5T_002973"
     CDS             3819..5075
                     /gene="yjeH"
                     /locus_tag="ACHX5T_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR8468069.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5091..5567)
                     /locus_tag="ACHX5T_002974"
     CDS             complement(5091..5567)
                     /locus_tag="ACHX5T_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR8468070.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            5904..7340
                     /gene="aspA"
                     /locus_tag="ACHX5T_002975"
     CDS             5904..7340
                     /gene="aspA"
                     /locus_tag="ACHX5T_002975"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR8468071.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7458..8759
                     /gene="dcuA"
                     /locus_tag="ACHX5T_002976"
     CDS             7458..8759
                     /gene="dcuA"
                     /locus_tag="ACHX5T_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR8468072.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8875..9213
                     /gene="cutA"
                     /locus_tag="ACHX5T_002977"
     CDS             8875..9213
                     /gene="cutA"
                     /locus_tag="ACHX5T_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR8468073.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9189..10886
                     /gene="dsbD"
                     /locus_tag="ACHX5T_002978"
     CDS             9189..10886
                     /gene="dsbD"
                     /locus_tag="ACHX5T_002978"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR8468074.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10923..11498
                     /locus_tag="ACHX5T_002979"
     CDS             10923..11498
                     /locus_tag="ACHX5T_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8468075.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11605..11680
                     /locus_tag="ACHX5T_002980"
     tRNA            11605..11680
                     /locus_tag="ACHX5T_002980"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11638..11640,aa:Phe,seq:gaa)
BASE COUNT         2889 a         3123 c         2959 g         3053 t
ORIGIN      
        1 ccataagcat caagggtgtt aataccggtg ttattcgcaa gagtaataat ttcattgcac
       61 tggcgctgaa aattaaagaa cctcgcaata aagaatcgtt gttctttatg tctgtcatgg
      121 aactgcgcga cctgctgatt gcgctggaaa gtcgtctgca tcaaaagcat aaactggatg
      181 ctgctgcacg ccttcagtat gagcaagctc gcgataaagt cattaagaaa atggcagaaa
      241 acatcccaga aattctggtt gatgagctta aaaatgccga tattaatcgt cgggttaaca
      301 cgctggaact taccgataat caaggcgaaa atcttacctt cgtattaacg ctacatgacg
      361 gtagcaaatg tgagttagtc gtcaatgagt tgcagattga aatgctggcg cgagcaatca
      421 ttcatgcaat taataatgcg gaaatgcgtg aactggcatt acgtattacc tcactgttag
      481 atttcctgcc gttatacgac gttgactgcc aggacaatgg caatctggaa tatgacacct
      541 attcccaacc ggaatggaaa cataacttgt tcgaccatta tctggcggtg ctttaccgct
      601 ttaaagatga aagtggcaaa gaacaattta gcggcgcagt ggtgaaaacg cgtgaagcaa
      661 cgccgggtaa agaaatagag gcgatcactc gccgaatgct tgatttcagc ccacgactga
      721 aaaaactcgc gggtgtgcca tgtcaggtct atgttcgcac cgtagcggcg aataacgccc
      781 aaccgctaac ccaggatcaa tgcttacgcg cgctgcatca cttacgtgta caatccacca
      841 gcaaaactgc accacaggcg aaataatctc atctttgcta atgcgaggct ggagccatcc
      901 agcctgaaat gactgataac gtgatcgttg gtcatgccgg atgcggcgta aacgccttat
      961 ccggcctaca aattctcaag cattcaatca gttgcaatgc ctgctgtagg cctgataagc
     1021 gaagcgcatc aggcaataat acgtttgtca cccagcctct ggatcacatc agttcgggca
     1081 cttataaacc tgaccgataa tctggctatc cgtcgggaca aaactggaca acattccctg
     1141 cgacgggctg ctgatgccat aaatcacgtt accgcccatt gcagccgcct ggttgcgcag
     1201 atcgtttgct gcgccgcgca tagaaccgcc ctcttctccg tgttgcccgg aaagccagtt
     1261 gctttgctta cctgtcgcag taccaatcag ctggcactct gcgcctggct gctcgtccac
     1321 aatgcgtaca ctctgaccgg cagcactcaa ttcgttgctg gagctacaac ccgccatcag
     1381 tagcgcggca ccgacaatcc ctgctaagta ttttacgtgc atgttattcc ccataatcaa
     1441 tgagctggac gccactcgcg tcatccgtgt ctgaatctta tactagaaag atgaccaaaa
     1501 gaaaaacccc cagacatttc tgcccggggg tttgtttatt tctgcgaagt gcagggcaat
     1561 tacatcatgc cgcccatgcc acccatgccg cccataccgc cagcagcgcc taagtcagct
     1621 gcatcgtttt tcggcaggtc ggtaaccatg cattcggtgg tgatcatcag gccagccaca
     1681 gaagctgcgt actgcagagc agaacgggtt actttggttg ggtccaggat acccatgtcg
     1741 atcatgttgc cgtattcttc ggttgctgcg ttgtaaccgt agttgccgtc gccgccttta
     1801 acggtgttag caacaacaga cggttcttcg ccgcagttca ggacgatctg acgcagcgga
     1861 gcttccattg cacgcagtgc aactttgata cccacgttct ggtcttcgtt ctgaccacgc
     1921 aggtcagcca gtttagacgc tacgcggatc agcgcaacac caccaccagc aaccacgcct
     1981 tcttctaccg cagcacgggt cgcgtgcagg gcatcttcaa cgcgtgcttt tttctctttc
     2041 atttcaactt cggtagcagc acctactttg ataactgcaa cgccgcctgc cagtttcgct
     2101 acgcgctcct gcagtttttc acggtcgtag tcagaagttg cttcttcaat ctgctgacgg
     2161 atctgagcaa cacggccctg gattgcagct tcttcaccca cgccatcgat gatagtggtg
     2221 gtgtctttgt tgatcacaac acgtttagcc tgacccaggt cttccagggt tgctttttcc
     2281 agctccatac cgatctcttc agagattacg gtaccgccag tcagggttgc gatatcctgc
     2341 agcatagctt tacgacgatc gccgaagccc ggagctttaa ccgcagcaac tttcacgatg
     2401 ccacgcatgg tgttaacaac cagagttgct agcgcttcgc cttcaacatc ttcagcgatg
     2461 atcagcagcg gtttgcctgc tttggcaaca gcttccagaa ccggcagcat ttcgcggatg
     2521 ttggagattt tcttgtcagc cagcaggatg aacgggcttt ccagttctac tgcgccagtt
     2581 tccggcttgt tgatgaagta aggagacagg tagccacggt cgaactgcat accttcaacc
     2641 acgtccagtt cgtcctgcag accggtaccg tcttcaacgg tgataacgcc ttctttaccg
     2701 actttgtcca tcgcttcagc gatcagttta cctacggttt cgtcggagtt agcggagatg
     2761 gtaccaacct gagcaatcgc tttagaatca gagcacggta cggacagcgc tttcagttct
     2821 tcaactgcag cggtaaccgc tttgtcgata ccacgtttca ggtccatcgg gttcatgccc
     2881 gcagcaacag ctttcagacc ttcagtgatg atagcctgag ccagtacggt tgcagtggtg
     2941 gtaccgtcgc ctgcagcgtc gttcgctttg gaggcaactt ctttcaccat ctgcgcaccc
     3001 atattttcga acttgtcttc cagttcgatt tcacgagcaa cggaaacacc atctttggtg
     3061 atggtcggtg caccgaaaga tttatccaga actacgttac ggccttttgg accgagggta
     3121 actttcactg catctgccag tacgtttacg ccgcgcagca ttttcacacg agcgtcgtta
     3181 ccgaatttta cgtctttagc tgccattatc tttattcctt aaattcgtat gttcagtgtc
     3241 gtgcgcggat tacgcttcaa caattgccag aatgtcgctt tcggacatga tcaacacttc
     3301 ttcattgtcg atcttctcgg atttcacacc gtagccatcg ttgaaaataa cgatgtcgcc
     3361 aactttcacg tccagcggct tcacttcgcc attttcaagg atacggccat tgccgacagc
     3421 cagcacttcg ccgcgggtgg atttagccgc tgcagagccg gtcagaacga tgccgccagc
     3481 agatttagtt tcaacttctt tacgcttgac gatcacgcga tcatgcaatg gacgaatatt
     3541 cattgataac tctcctttga gaaagtccgt atctgttatg ggtgacgccg gagcttacgt
     3601 ggtttcccgg ctggtgacca gagaaatggg gatgaggctt cgccccttca agggggaaaa
     3661 agaaaaaaaa ttctgatcag cacaaaatcg ggtgaaaacc ctgattcacc tcacatttca
     3721 tcgcaatttc ttcatcgccg tcataagcga atctgattgt gctaccatcg aaaatctacg
     3781 cagttgccca aaatttgggc gcaatcggat atcaacccat gagtggactc aaacaagaac
     3841 tggggctggc ccagggcatt ggcctgctat cgacgtcatt attaggcact ggcgtgtttg
     3901 ccgttcctgc gttagctgca ctggtagcag gcaataacag cctgtgggcg tggcccgttt
     3961 tgattatctt agtgttcccg attgcgattg tgtttgcgat tctgggtcgc cactatccca
     4021 gcgcaggcgg cgtcgcgcac ttcgtcggta tggcgtttgg ttcgcggctt gagcgagtca
     4081 ccggctggct gtttttatcg gtcattcccg tgggtttgcc tgccgcacta caaattgccg
     4141 ccggattcgg ccaagcaatg tttggctggc atagctggca actgttgttg gcagaactcg
     4201 gtacgctggc gttggtgtgg tatatcggta ctctcggtgc cagttccagt gctaatctac
     4261 aaaccgttat tgccggactt atcgtcgcac tgattgtcgc tatctggtgg gcgggcgata
     4321 tcaaacctgc gagtatcccc ttccccgcgc caggaaatat cgaacttacc gggttatttg
     4381 ctgcgttatc agtgatgttc tggtgttttg tcggtctgga agcatttgcc catcttgcct
     4441 cggaatttaa aaatccagag cgtgattttc ctcgtgcttt gatgattggt ctgctgctgg
     4501 caggattagt ctactggggc tgtacggtag tcgtcttaca cttcgacgcc tatggtgaac
     4561 aaatggcggc ggcagcatcg cttccaaaaa ttgtagtaca gttgttcggt gtaggagcgt
     4621 tatggattgc ctgcgtaatt ggctatctgg cctgctttgc cagtcttaac atttatatac
     4681 agagcttcgc ccgcctggtc tggtcgcagg cgcaacataa tcctgaccac tacctggcac
     4741 gcctctcttc tcgccatatc ccgaataatg ccctcaatgc ggtgcttggc tgctgcgtgg
     4801 tgagcacgtt ggtaattcat gccttagaga tcaatctgga cgctcttatt atttatgcca
     4861 atggcatctt tattatgatt tatctgttat gcatgctggc aggctgtaaa ttattgcaag
     4921 gtcgttatcg actactggcg gtggttggcg ggctgttatg cgttctgtta ctggcaatgg
     4981 tcggctggaa aagtctctat gcgctgatca tgctggtggg gttatggctg tttctgccaa
     5041 aacgaaaaac gccggaaaat ggcataacca cataatccgg cgtttcgaca ttaatcctgg
     5101 cgatcgtctt tatgatcaag gcggtcgcgg tcatcatcct ttcgctgata ctcaccatca
     5161 aaagtattac cgccaccggt cccggcgcta aaaccgccgc ctggcatgcg agaaaagcgc
     5221 aaatgtggca tcaacttcac tgtcagatgc ttttgcaccg gcggcaataa aagcagaaga
     5281 ccgaggaagt cggtgaaaaa gcccggtaat aaaagcagca aaccggcaat aatcagcgaa
     5341 acacttttaa tcatctccgc cgcagggttt tcacccgccg ccattttttg ctgcatcagc
     5401 acgaaattct taaagccctg gttacgtacc agtgacatac cgataactga cgtgaatata
     5461 accagcacga gggtcagcaa tacccccaat acatgggcaa cctgaataaa gattgaaatc
     5521 tcaatataga cataaaggaa aatggcaata aaaggtaacc agcgcaaagg tttctcctgt
     5581 aatagcagcc ggttaaaccc ggctacctga atgggttgcg aatcgccttc agcttatatt
     5641 gtggtcatta gcaaaatttc aagatgtttg cgcaactatt tttggtagta atcccaaagc
     5701 ggtgatctat ttcacaaatt aataattaag gggtaaaaac cgacacttaa agtgatccag
     5761 attacggtag aaatcctcaa gcagcatatg atctcgggta ttcggtcgat gcaggggata
     5821 atcgtcggtc gaaaaacatt cgaaaccaca tatattctgt gtgtttaaag caaatcattg
     5881 gcagcttgaa aaagaaggtt cacatgtcaa acaacattcg tatcgaagaa gatctgttgg
     5941 gtaccaggga agttccagct gatgcctact atggtgttca cactctgaga gcgattgaaa
     6001 acttctatat cagcaacaac aaaatcagtg atattcctga atttgttcgc ggtatggtaa
     6061 tggttaaaaa agccgcagct atggcaaaca aagagctgca aaccattcct aaaagtgtag
     6121 cgaatgccat cattgccgca tgtgatgaag tcctgaacaa cggaaaatgc atggatcagt
     6181 tcccggtaga cgtctaccag ggcggcgcag gtacttccgt aaacatgaac accaacgaag
     6241 tgctggccaa tatcggtctg gaactgatgg gtcaccagaa aggtgaatat cagtacctga
     6301 acccgaacga ccatgttaac aaatgtcagt ccactaacga cgcctacccg accggtttcc
     6361 gtatcgcagt ttactcttcc ctgattaagc tggtagatgc gattaaccaa ctgcgtgaag
     6421 gctttgaacg taaagctgtc gaattccagg acatcctgaa aatgggtcgt acccagctgc
     6481 aggacgcagt accgatgacc ctcggtcagg aattccgcgc tttcagcatc ctgctgaaag
     6541 aagaagtgaa aaacatccaa cgtaccgctg aactgctgct ggaagttaac cttggcgcaa
     6601 cagcaatcgg tactggtctg aacacgccga aagagtactc tccgctggca gtgaaaaaac
     6661 tggctgaagt cactggcttc ccatgcgtac cggctgaaga cctgatcgaa gcgacctctg
     6721 actgcggcgc ttatgttatg gttcacggcg cgctgaaacg cctggctgtg aagatgtcca
     6781 aaatctgtaa cgacctgcgc ttgctctctt ctggtccacg tgccggcctg aacgagatca
     6841 acctgccgga actgcaggcg ggctcttcca tcatgccagc taaagtaaac ccggttgttc
     6901 cggaagtggt taaccaggta tgcttcaaag tcatcggtaa cgacaccact gttaccatgg
     6961 cagcagaagc aggtcagctg cagttgaacg ttatggaacc ggtcattggc caggccatgt
     7021 tcgaatccgt tcacattctg accaacgctt gctacaacct gctggaaaaa tgcattaacg
     7081 gcatcactgc taacaaagaa gtgtgcgaag gttacgttta caactctatc ggtatcgtta
     7141 cttacctgaa cccgttcatc ggtcaccaca acggtgacat cgtgggtaaa atctgtgccg
     7201 aaaccggtaa gagtgtacgt gaagtcgttc tggaacgcgg tctgttgact gaagcggaac
     7261 ttgacgatat tttctccgta cagaatctga tgcacccggc ttacaaagca aaacgctata
     7321 ctgatgaaag cgaacagtaa tcgtacaggg tagtacaaat aaaaaaggca cgtcagatga
     7381 cgtgcctttt ttcttgtgag cagtaactta aaaataacaa tctaatatca acttgttaaa
     7441 aaacaaggaa ggctaatatg ctagttgtag agctcatcat agttttgctg gcgatcttct
     7501 tgggcgccag attgggggga ataggtattg gttttgcagg cggattgggg gtgctggttc
     7561 ttgccgctat tggcgttaaa cccggtaaca tcccgttcga tgtcatttcc attatcatgg
     7621 cggttatcgc cgctatttct gccatgcagg ttgctggcgg tctggactat ctggttcatc
     7681 agacagaaaa gctgctgcgc cgtaacccga aatacatcac gatcctcgca ccgatcgtga
     7741 cctatttcct gactatcttt gctggtactg gcaacatctc tctggcgaca ctgccagtta
     7801 tcgctgaagt tgcgaaggaa caaggcgtta aaccttgccg tccgctctct actgcagtgg
     7861 tatccgcgca gattgcgatc accgcatcgc caatctcagc ggcagtggtt tacatgtctt
     7921 ccgtgatgga aggtcatggc atcagctacc tccatctgct ctccgtggtc atcccgtcca
     7981 ccctgctggc ggttctggtg atgtccttcc tggtcactat gctgttcaac tccaaactct
     8041 ctgacgatcc gatttatcgc aagcgtctgg aagagggctt ggttgaactg cgcggtgaaa
     8101 agcagattga aatcaaatcc ggtgcaaaaa cgtccgtctg gctgttcctg ctgggcgtag
     8161 ttggcgtggt tatctatgca atcatcaaca gcccaagcat gggtctggtt gaaaaaccgc
     8221 tgatgaacac caccaacgca atcctgatca tcatgctcag cgttgcaact ctgaccaccg
     8281 ttatctgtaa agtcgatacc gacaacatcc tcaactccag caccttcaaa gcaggtatga
     8341 gcgcctgtat ttgtatcctg ggtgttgcgt ggctgggcga tactttcgtt tccaacaaca
     8401 tcgactggat caaagatacc gctggtgaag tgattcaggg tcatccgtgg ctgctggccg
     8461 tcatcttctt ctttgcttct gctctgctgt actctcaggc tgcaaccgca aaagcactga
     8521 tgccgatggc tctggcactg aacgtttcac cgctgaccgc tgttgcttct ttcgctgcgg
     8581 tgtctggtct gttcattctg ccgacctacc cgacgctggt tgctgcggta cagatggatg
     8641 acacgggtac tacccgtatc ggtaaattcg tcttcaacca tccgttcttc atcccgggta
     8701 ctctgggtgt tgccctggcc gtttgcttcg gcttcgtgct gggtagcttc atgctgtaat
     8761 gatcaatcgc ggggcgttca cgccccgctt tctttcccgc cgactaacat ccttcccccg
     8821 tccgttgtat agtgacctct ctcttgcggt tccatctgtt cttgcgaggt gtttatgctt
     8881 gatgaaaaaa gttcgaatac cacgtctgtc gtggtgctat gtaccgcacc ggatgaagcg
     8941 acagcccagg atttagccgc caaagtgctg gcggaaaaac tggcggcctg cgcgaccttg
     9001 atccccggcg ctacctctct ctattactgg gaaggtaagc tggagcaaga atacgaagtg
     9061 cagatgattt taaaaactac cgtatctcac cagcaggcac tgctggaatg cctgaagtct
     9121 catcatccat atcaaacccc ggaacttctg gttttacctg ttacacacgg agacacagat
     9181 tacctctcat ggctcaacgc atctttacgc tgattctgct actttgcagc acttccgttt
     9241 ttgccggatt attcgacgcg ccgggacgtt cacaatttgt ccccgcggat caagcctttg
     9301 cttttgattt tcagcaaaac caacatgacc ttaatctgac ctggcagatc aaagacggtt
     9361 actacctcta ccgtaaacag atccgcatta cgccggaaca cgcgaaaatt gccgacgagc
     9421 agctgccgca aggcgtctgg catgaagatg agttttacgg caaaagcgag atttaccgcg
     9481 atcggctgac gcttcccgta accatcaacc aggcgagtgc gggagcgacg ttaactgtca
     9541 cctaccaggg ctgtgctgat gccggtttct gttatccgcc agaaaccaaa accgttccgt
     9601 taagcgaagt ggtcgccaac aacgcagcgt cacagcctgt gtctgtttcg cagcaagagc
     9661 agcacaccgc gcaattgccc ttttccgcgc tctgggcgtt gttgatcggt attggtatcg
     9721 cctttacgcc atgcgtgctg ccaatgtacc cactgatttc tggcatcgtg ctgggtggta
     9781 aacagcggct ctccactgcc agagcattgt tgctgacctt tatttatgtg caggggatgg
     9841 cactgactta cacggcgctg ggtctggtag ttgccgccgc agggttacag ttccaggcgg
     9901 cgctacagca cccatacgtg ctcattggcc tcgccatcgt ctttaccttg ctggcgatgt
     9961 caatgtttgg cttgtttact ctacaactcc cctcttcgct gcaaacacgt ctcacgttga
    10021 tgagcaatcg ccaacagggc ggctcaccta gcggtgtgtt tgttatgggg gcgattgccg
    10081 gactgatctg ttcaccatgc accaccgcac cgcttagcgc gattctgctg tatatcgccc
    10141 aaagcgggaa catgtggccg ggcggcggca cgctttatct ctatgcgttg ggcatgggcc
    10201 tgccgctgat gctaattacc gtctttggta accgcttgct gccgaaaagc ggcccgtgga
    10261 tggaacaagt caaaaccgcg tttggttttg tgatcctcgc actgccggtc ttcctgctgg
    10321 agcgagtgat tggtgatgta tggggattac gcttgtggtc ggcgctgggt gtcgcattct
    10381 ttggctgggc ctttatcacc agcctacagg ctaaacgcgg ctggatgcgc gtggtgcaaa
    10441 taatcctgct ggcagcggca ttggttagcg tgcgcccact tcaggattgg gcatttggtg
    10501 cgacgcatac cgcgcaaact caaacgcatc tcaactttac acaaatcaaa acggtagatg
    10561 agttaaatca ggcgctcgtt gaagccaaag gcaaaccggt gatgttagat ctttatgccg
    10621 actggtgcgt cgcctgtaaa gagtttgaga aatacacctt cagcgacccg caggtgcaaa
    10681 aagcgttagc agacacggtc ttacttcagg ccaacgtcac ggccaacgac gcacaagatg
    10741 tggcgctgtt aaagcatctt aatgtccttg gcctaccgac aattctcttt tttgacggac
    10801 aaggccagga gcatccacaa gcacgcgtca cgggctttat ggatgctgaa accttcagcg
    10861 cacatttgcg cgatcgccaa ccgtgaacga cacttgcagt gggaaagacg gaggaaaata
    10921 gcgtgcaacg tgaagatgta ctgggagaag ccctgaaatt attagaatta caagggattg
    10981 ccaacaccac gctggagatg gttgctgaac gtgtggatta tccactggac gagctacgcc
    11041 gcttctggcc agacaaagag gcaatcctct acgatgcgct gcgttatctt agccaacaga
    11101 tagatgtctg gcgtcgtcag ctgatgctgg acgaaacgca aaccgccgaa caaaagctgc
    11161 tggcacgtta tcaggcgtta tcggagtgcg ttaaaaacaa ccgctatccg ggctgtctat
    11221 ttatcgctgc ctgtacgttt tatcccgatc ctggccaccc tattcatcaa ctggccgatc
    11281 agcaaaaaag cgctgcctac gatttcaccc acgaactgtt aaccacgctg gaagttgacg
    11341 atccggcgat ggtagcaaag cagatggaac tggtgctgga aggctgttta agccgaatgc
    11401 tggtgaatcg tagccaggcg gatgtcgaca ctgcacatcg gctggcggaa gatatcctgc
    11461 gcttcgcccg ctgccgccag ggtggtgcat tgacctgaca gaaacacaga aaagaagcga
    11521 tttgccgcaa tcttaagcag ttgaatcgct tttactgaaa ttaggttgac gagatgtgca
    11581 gattacggtt taatgcgccc cgttgcccgg atagctcagt cggtagagca ggggattgaa
    11641 aatccccgtg tccttggttc gattccgagt ccgggcacca aattcatatc aacggacctc
    11701 cacggaggtc cgtttttcgt ttcaggacgc cacgatttaa gcgttctgcc tccaaatcaa
    11761 ttctaccgaa ctcaaccaga ttctccccac atcaccagca atttgcgagc atatcccaat
    11821 ttgggaaaat ttgttcctga gctatagcac taactgacgt gaaatgtcgt gcggccccgt
    11881 gatgctgttg aacgtcaaat gacgtcatca ggagcgtaac gtatccataa agcacaacac
    11941 cggtctgaac gccgactgat gagattttct gaatgagaac aaagagaaat gtgtcagtcc
    12001 gcttgctcat gcaaagacta acaa
//
LOCUS       DAZSIO010000120        12014 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZSIO010000120 DAZSIO010000000
VERSION     DAZSIO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.120"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(5..508)
                     /locus_tag="ACHX5T_002981"
     CDS             complement(5..508)
                     /locus_tag="ACHX5T_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR8468076.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
     gene            complement(553..1516)
                     /locus_tag="ACHX5T_002982"
                     /pseudo
     CDS             complement(553..1516)
                     /locus_tag="ACHX5T_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(1830..2966)
                     /gene="hemW"
                     /locus_tag="ACHX5T_002983"
     CDS             complement(1830..2966)
                     /gene="hemW"
                     /locus_tag="ACHX5T_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR8468077.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(2959..3552)
                     /locus_tag="ACHX5T_002984"
     CDS             complement(2959..3552)
                     /locus_tag="ACHX5T_002984"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR8468078.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(3560..3850)
                     /gene="yggU"
                     /locus_tag="ACHX5T_002985"
     CDS             complement(3560..3850)
                     /gene="yggU"
                     /locus_tag="ACHX5T_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR8468079.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(3847..4413)
                     /gene="yggT"
                     /locus_tag="ACHX5T_002986"
     CDS             complement(3847..4413)
                     /gene="yggT"
                     /locus_tag="ACHX5T_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR8468080.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(4431..5135)
                     /gene="yggS"
                     /locus_tag="ACHX5T_002987"
     CDS             complement(4431..5135)
                     /gene="yggS"
                     /locus_tag="ACHX5T_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR8468081.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            5153..6133
                     /locus_tag="ACHX5T_002988"
     CDS             5153..6133
                     /locus_tag="ACHX5T_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR8468082.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            complement(6309..6725)
                     /gene="ruvX"
                     /locus_tag="ACHX5T_002989"
     CDS             complement(6309..6725)
                     /gene="ruvX"
                     /locus_tag="ACHX5T_002989"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR8468083.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(6725..7288)
                     /locus_tag="ACHX5T_002990"
     CDS             complement(6725..7288)
                     /locus_tag="ACHX5T_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR8468084.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(7397..8347)
                     /gene="gshB"
                     /locus_tag="ACHX5T_002991"
     CDS             complement(7397..8347)
                     /gene="gshB"
                     /locus_tag="ACHX5T_002991"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR8468085.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(8360..9091)
                     /gene="rsmE"
                     /locus_tag="ACHX5T_002992"
     CDS             complement(8360..9091)
                     /gene="rsmE"
                     /locus_tag="ACHX5T_002992"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR8468086.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(9171..9878)
                     /gene="endA"
                     /locus_tag="ACHX5T_002993"
     CDS             complement(9171..9878)
                     /gene="endA"
                     /locus_tag="ACHX5T_002993"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR8468087.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(9973..10470)
                     /locus_tag="ACHX5T_002994"
     CDS             complement(9973..10470)
                     /locus_tag="ACHX5T_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR8468088.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            complement(10547..11941)
                     /gene="galP"
                     /locus_tag="ACHX5T_002995"
     CDS             complement(10547..11941)
                     /gene="galP"
                     /locus_tag="ACHX5T_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR8468089.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         2942 a         3081 c         3059 g         2932 t
ORIGIN      
        1 tccattaact ggcctcctgc gcaggttgtt tcagttgttg aatgctttga ttaataaaac
       61 acaaggcata aaacatcatt accactccac ttaatggcag aatgatatat acgtatccca
      121 tcggtatttg catagcggca gatacctgtg atgttgccag tgttttatca attaatttca
      181 ggccaccagt cacaatgacc gaaccggcaa aacctaaaat gagaacgtta atgactatac
      241 taagaagtaa ttgtttgcgc ttattcaggg cgagagcaaa taaatcaata gagagatgtc
      301 gctgcgcgcc gacggtatat gcagcaccta ataatcctac ccagatcatc aaaaagcgtg
      361 ccagctcatc ggttaatgta cttggctggt taagtacgta gcggctaaat acttgccaga
      421 ctacgcatac aaccagagca agcattacgg cgacagaaaa cgtggcaatg acgcggtcta
      481 ccgcaagttt caggcggtta aatatcattg taagcatcct ggaaaatcag ggccagaggc
      541 tggcccggaa tattattgag cagcgttgtc gaatttctct aacaaagcag cttgttttgg
      601 atcttttttg aagtcgtcaa acagaggttg cacggcttta cggaatggcg ctttatccac
      661 ttttacaatt tcacccccca tagctttcgc ctgagcacgt gtgtcagcgt caattttttc
      721 ccacaatttt tgttgataag cttcggattt cttcgccgct gtttcgagga tttgttgctg
      781 ttctggggtt aatttattcc atgttttggt ggagataacc aaaaagtcag gaattgaggc
      841 gtgttcatct tcagaaaaaa ctttggcaat ttcaatatgt cgagtctgca tccaggaagg
      901 cacgttattt tctgcgccat ctactacacc ttgctgcatc gcagtataaa cttcgccaaa
      961 tgaaattgga gtgggtgaac cacccatcag ttcaatcatt ttgatggtgg tcggacttgg
     1021 ctgaacacga attttcagtc ctttaagatc ttctggcttt gtaatcggtt ttttcgcata
     1081 gaaactgcga gtacccgcta cataagctga taaagcaaaa aagcctttgt cttttgttga
     1141 gtccataatt tctttaccaa cttcaccaaa aacaacttaa taagaacggc aaattataga
     1201 tagcataaat attatcaaaa gactccagat cactggcgga acctttggtc atatccagtg
     1261 cgccattttg caacaattcc atagtttctc gcgcattacc catctggctg ctgggataaa
     1321 tacggatcac catattacct tttgacagct gttttacctc tttcgccagc tcttcaaaac
     1381 tctgatgtac aacatgactt ctttccaggt tgtgtgctaa ctttaacgcc actttttctg
     1441 cggcgtttac gccagtgcta accagagcca gcaaggatgc gcagactaat gagcgggtaa
     1501 atgacctcat tttcataaaa aaactccctg ttgaaggatg gaaattggtt gatatctaat
     1561 tgtgcttatc agtgatttgt gcgacaaatt ggttaacctg tctgacaact tgtctatgtt
     1621 tatcacaact tgaaataaag tcagaaatta aatattaaat ttgtgcgcca ggttgtacat
     1681 atttataatc aatgaatgat tagtaaatat tcactaaata tatataataa atgtcgtcga
     1741 tatttattca gtaagaaata tatttctgcc gaggttaatt aacgggggga taaaagaagt
     1801 gattaatgcg cccgcaggcg cattaatgat tactcagcga ggaataattc aagaagtgag
     1861 tttaagaata atttaccatg ttccgttatc tgccagtaat ccgcacattc ggtgagataa
     1921 ccctgggcaa tcgcctcgtc taactgtggg cgaatcacat cttcgcaaag cccggtatac
     1981 gcaataaact ccacgcgcgg cgcggcctcc agcagacgga agcgattcat aaagaactca
     2041 aacggcttat ctgcggcttc gacatcacgc tggctttcca gataccttcc ttgcataaaa
     2101 ccacgaggat gacgcgtttt agtggtacgc agaatgcgcc catccgggaa ggtcactttg
     2161 ccgtgcgcgc cgcagccaat accgatgtag tcaccaaagc gccagtagtt gagattgtgc
     2221 tggcactgat aaccgggttt ggcgtaagcg gaagtttcat attgctgata acccgctgcg
     2281 gttaataact gatgcccctg ttcgaaaata tcccacagcg cgtcatcgtc cggcagcacc
     2341 ggcggtcgcg agccaaacag cgtattaggt tcgatagtca gttgatacca ggaaagatgc
     2401 ggcggattca gttcaatggc ctggcgtaga tcgccaagcg cctcttccag tgattgatcc
     2461 ggtagtccat gcatcaaatc aaggttaaag ctacgtaacc ctaaaccgct cgccagcttt
     2521 gccgctcgtt tcgcttcttg cgggccatga atacgcccaa gtcgtttcag cttttcttcg
     2581 ctaaaactct gcacaccaat agagatgcgg tttacaccag cacgctgata atcgacaaag
     2641 cgatccgctt ctaccgtgcc tgggttcgct tccatagtaa tttctgcatc cgctgccagc
     2701 ggcaaacgcg cacgcacgcc gtctagcagc gtttgcatcg ccgggccgga aagcaggctc
     2761 ggcgtaccac cgccaataaa gattgtcttt acttcacggc cctgagcgta agccacatca
     2821 ttgtccagat cgcaaagcag atgctgaaca taatcgtcgt gcggcacttc tcctttcaac
     2881 gcgtgagagt tgaaatcgca gtacgggcat ttctgcacgc accacgggat gtgaatgtag
     2941 agactcagcg gcggtaattt aaccattacg taaagcgtcc agcagcagtt tcaacgcctg
     3001 accacggtgg gaaatggcgc tcttttcttc gcgggtcagt tcggcggcgg ttttcccttc
     3061 ggaaggtacg aagaagattg gatcataacc aaagccacca gtgcccgctg gttcgcgagt
     3121 aatcacgccc ggccagctgc cgtggcatac cagcggagtc ggatcttccg cgtgacgcag
     3181 atataccagc acgcagtgga aacgcgcctg acgttggtcg tccggtacgt ctttcagtgt
     3241 ttccaacagt ttttgcagat tcttttgatc ggtcgcgtct tcaccggaat aacgcgcgga
     3301 gtaaataccc ggcgcgccac ccagcgcatc taccgccaga ccagagtcgt cggcaattgc
     3361 cggtaaaccg gtcacttttg ccgcatggcg cgctttcaga atcgcgtttt cgataaaggt
     3421 taggccggtt tcttcagcgg aatcaacgcc gaggtctgtt tgggccacga tatcaagacc
     3481 aaagtcgcta agcagcgacg ccagctcacg cactttaccg gcattgccgg ttgcgaggac
     3541 aactttttgc ataggatacc taattaatta acgccgcgat ttctggcggg atttgttgcg
     3601 gattaatgat tttaatttgt ttgtggcggc caagttcgcc tttttcaatc accacctggc
     3661 ttttggcaac ccggaattgc ttaccgagaa acttcaccag atgactgttg gcctggccgt
     3721 caaccggcgg cgcggtaatg gcgactttaa cttcgtcgcc atgtaaaccg acaatagaat
     3781 cacggctggc tttcggctga atatagagcc gtaaaaccag accgtcatca ttaactgtta
     3841 cggcactcat aacgccatcc acagccccgg cagcagcatg tttccggttg cctgtaatac
     3901 ttctgcgaca cccatattga tggcatacag cagcagaacg aggatcatcg gcgagaaatc
     3961 aatcccaccc attgccggta gcaagcggcg aatcgggcgc agcagcggat cggccagctg
     4021 aatcagcacg tattcaatcg ggctacgacc ctggcttacc cagctcataa tcgccatcac
     4081 cagcaggacc cagaaaatca gcaagccgat ggttttcagc agaatcagta aaccggcaat
     4141 ccagatgatt ggcaggaagg tcaccacttc aaacagcaca atggctttga taaaactgag
     4201 aatataggca accagcagcg aggcgctgtc aatgggaccc attgccggaa taacgcggcg
     4261 cagtggcccg ataattggct gcgctacctt cactacaaac tgtgagaagg gggtgtaaaa
     4321 atcacaatga gcacactgca tccagatgcg taataacagc accattgtat acagctcaat
     4381 gaccgttgaa agcaggaaag tcaacgtatt catggcgttc ctttaattcc ttatttttta
     4441 gagtaatcac gcgcaccaaa aattgcagtg ccgatacgaa ccatcgtgct acctgccgca
     4501 atggcggctt ccatatcgtc cgacattccc agagagagcg tgtcgatatg cgggtagcgc
     4561 gttttcagtc cggcaaatgc tacagccatt tggcgtgcaa cttcaaactg ccttacatat
     4621 tctgactcaa gcgcagggat tgccatcagc ccgcgcagac gtaaacgcgg tagttcagcg
     4681 accgccgctg ccagctcgtc cagttctgcc agttgaatcc cggacttact gttttcatca
     4741 ctaatattaa tttgaatcag tacgttaaga gggggaagtt ctgccgggcg ctgctcgttg
     4801 aggcgggtag caatgcgcaa acggtcgatg gtatgacacc agtcgaaatg ctctgccact
     4861 aggcggcttt tattagactg caacgggcca ataaaatgcc attctaatcc tgttacgccc
     4921 agttcctgaa agtggcgaat tttatctacc ccttcctgaa cgtagttttc accaaattga
     4981 cgctgcccgg catcaatggc ttctgcgatg gcgctcgcag gttttgtttt actgactgca
     5041 agcagcgtaa tttcttctgg agaacggccg caacgcgttg cagccgctga gattttgtcc
     5101 cggacctgtg ccaggttatg cgcaatatcg ttcattttcc gaggatctta gtatgaatat
     5161 ggaagaaatt gtggccctta gtgtaaagca taacgtctcg gatctacacc tgtgcagcgc
     5221 ctggcccgca cgatggcgca ttcgtggcag aatggaagct gcgccgtttg atgcgccgga
     5281 cgtcgaagag ctactgcggg agtggctgga tgacgatcag cgggcaatat tgctggaaaa
     5341 tggccagctg gattttgccg tgtcgctggc ggaaaaccag cggttgcgtg gcagtgcgtt
     5401 cgcgcaacgg caaggtattt ctctggcatt acggttgtta ccttcgcact gtccacagct
     5461 cgaacagctt ggtgcaccac cggtattgcc ggaattactc aagagcgaga atggcctgat
     5521 tctggtgacg ggggctacgg ggagtggcaa atctaccacg ctggcggcga tggttggcta
     5581 tcttaatcaa catgccgatg cgcatattct gacgctggaa gatcctgtgg aatatctcta
     5641 tgccagccag cgatgtttga tccagcagcg ggaaattggt ttgcactgta tgacgttcgc
     5701 atcggggttg cgggctgcat tgcgggaaga tcctgatgtg attttgctcg gagagctacg
     5761 tgacagtgag acaatccgtc tggcgctgac ggcggcagaa accgggcatc tggtgctggc
     5821 aaccttacat acacgtggtg ccgcgcaggc agttgagcga ctggtggatt catttccggc
     5881 gcaggaaaaa gatcccgtac gtaatcaact tgcgggtagt ttacgggcag tgctgtcaca
     5941 aaagctggaa gtggataaac aggaaggacg cgtggcgctg tttgaattac tgattaacac
     6001 tcccgcggtg gggaatttga ttcgcgaagg gaaaacccac cagttaccgc atgttattca
     6061 aaccgggcag aaggtgggga tgttaacgtt tcagcagagt tatcagcagc gggtggggga
     6121 agggcgtttg tgacgttgcc atgcccgatg cgcaagttta tagggcctat aaaggcaata
     6181 aatgtctgat gcgacgctaa cgcgtcttat cagacctaca gccgctgcca gatgtgtgct
     6241 ggataaggcg ttcacgccgc atccggcaag atggcggtaa cgactatccg ccaggctttt
     6301 agatcgcctt aatatccctg ctcgaaatag ctttcgagaa taataaccgc agaggcagag
     6361 tcaactttgc ctttgttgag cgcccgatag ccgccctgtt caaaaagacc ggaacgggct
     6421 tccacagtgc taagacgctc gtcatggagc tttacttcaa caccgaaacg accatgaata
     6481 cggttggcaa atttgcgcgc tctggcggtt aacggctgtt cggtgccgtc catattcagc
     6541 ggcaaaccga cgatgatttc gtcaggctgc cactctttca gtaaacgttc gatgaggttc
     6601 cagtccggcg taccgtcctg cgctttaatt gcaggcaaag ggcgagcggt gccggtaatg
     6661 cgttggccga ccgctacgcc aatgcttttg gtgccgaagt cgaaggcgag taaggttcca
     6721 ctcatcaggc gtgtcctgcc acaccaggca tggtgagaat atccacacca atcagttttg
     6781 ccgcctcgcg ccagcggtcg gcaatcggcg ttttgaacag aatattcaga tccgccgggg
     6841 ccgttagcca cgcgttatcg agaatttctt gttccagttg acctttttcc caggaggcat
     6901 agcccagagc caccaatacg tcagacggtt gtttatcggt gccgagcgtt tccagcacat
     6961 cgcgggaagt ggtcatcacc gtgttgtctg aaatgcgaat gctggaagca aaattggagg
     7021 gcggagtatg caaaataaac ccgcgatctt cagccagtgg gccgccgagc ataaccggtt
     7081 tatccagacg gattgattca tcacgcggct ccggcgtgat cttcagcttt tccagaatcc
     7141 cttcaatttt gagattttcc agcggcttgt tgacgatgat ccccattgca ccattggtat
     7201 tatgttcgca aatgtagacc acggaacgac ggaaaatcgg atcctggaga gcaggcatgg
     7261 caataagaaa gtgatgctgt aaattcattg tcagaggttc tgtttcctgg ttcaaaaaag
     7321 caacagcgcc cagtatgcgt ggaaagaagg gcgctgtcac tcagagtctc aacgagatcc
     7381 ttctcgctaa ggtgggttac tgctgctgta aacgtgcttc gatggcatcc attaacattc
     7441 cggtgatcga caccggaaac tctgcttcaa tctcacgaat acaggttggg ctggtgacgt
     7501 taatttcagt cagacggtcg ccgatgatat ccagaccaac aaaaatcagc cctttttctt
     7561 tcagcgtcgg cccgatctga cgggcgattt tccagtcact ttccgtcagc ggacgaggtt
     7621 caccgcgacc accggcagcc agattgccac gggtttcgcc cccctgcgga atacgcgcca
     7681 ggcagtacgg taccggctcg ccatccacca ccagcacgcg tttgtcgcca tctttaatgg
     7741 ctggcaggta attttgcgcc atgcagtagc aagtgccatg ctcagtcagg gtttcggcaa
     7801 tcacgccgag gtttggatcg ccttctttca cgcggaaaat cgacgcgccg cccataccgt
     7861 ccagcggctt aagaatgatg tcgctgtgtt tctcccagaa cgcttttagc tgcgctttat
     7921 tgcgcgtaac cagcgtttct ggcgttaagt cagagaacca ggcggtaaac agtttctcgt
     7981 tacagtcgcg caggctctgc ggcttgttaa cgatcagcgt ccctttctct tcggcacgtt
     8041 ccagaatata ggtcgcgtag ataaactcgg tatcaaacgg cgggtcttta cgcatcagga
     8101 tcacatcgag atcggccagc ggcagatcct gttcaccgac gaacgaaaac cactcttcgt
     8161 agttctgctt cacgttcagc gtgcgggtat gggcgcgggc ttcgccattg atcagataca
     8221 gatcgcccat ctccatatag tgaagttcgt aaccacgacg ctgtgcttcc agcaacatag
     8281 cgaaactgga atctttcttg atgttgatgt ttgcgatggg gtccatcacg atgccgagct
     8341 tgatcattat tcttctccgt tagcccaaat cgccaaatcg tacttgtagc gcggtaatgg
     8401 cggtgagcgc agttgtctct gtacgcaaaa cgcgaggtcc caacaggata tcagtaaatt
     8461 gatagcgggc agtcatggca atttcatctg ccgataaacc gccttccggg ccaatcagca
     8521 ggcggacgcg ttcaaccggt aaaggcaacg tattgatgct gttactggcg cgcgggtgaa
     8581 gattcagttt cagtccttca tcctgctctg cacaccaggc ttccagatcc atcgctggac
     8641 ggatttccgg cacccggtta cgaccacact gctcacaggc agcaattgca atcttctgcc
     8701 actgctgaag cttcttgttc agacgttcac tatccagttt aacgccgcag cgctcagaaa
     8761 aaagtggcgt aatgaggctt acaccgagtt cgatcgattt ctggatagta aattccattt
     8821 tttcaccacg cgacatcacc tgaccgaggt gaatatgcag cggagattcg cgatcgtcga
     8881 tctggccttc cagcaccttc acttccacgc tttttttgct ggcgctggta atttcggcgt
     8941 caaagacctg gttgctaccg tcaaacaatt gcaacgcctg ccccggcccc atgcgcagta
     9001 cgcgcccgat atggttggcg gcatcttcgc aaagcgcgat gtgagaatgg ctggtcagtg
     9061 gttcaggatg ataaatgcgg gggatacgca tagttaaaaa tccgcgtcgt ctccccacgc
     9121 ggttgtacgc gtggggtagg ggttaacaaa aagaatcccg ctagtgtagg ttagctcttt
     9181 cgcgcctggc aagcgcgttg cacatacggg ttatgattgc cctgcacctt cgcgatgcgt
     9241 tcatcgcgct cgcactccca gtcggtaacc ggatacatct tgttccatgc gttgaacagc
     9301 tgcgtttgct ggcgagagag tgtcaggttg tattggtcgc gcatatagaa gtaggtgcgc
     9361 gcaatggcac cgcgtgcacg cgctggtggt tcggcagctt tttctttgaa atcgaccttc
     9421 atggcgcatt gaccgtactg gccttcgccg ccattccact ggctgtacat aaaattgccg
     9481 cgatcgccat tcacctcgcc gactgacggc tgcaggttat gcatatcgct ttccatcttg
     9541 cgatagaccg gatctttagc gcagttttta cgtccaccgt cctgccagca ctggcgctgg
     9601 tgaccgaatt gccaggcggg aacgacgtgt tcccactcta cgcggctggc gcggttttca
     9661 tttttgcgca cctgatagcc gcacgattgc agatcaacaa cgcctttttt gccctgccag
     9721 ttaattttac atccgcaata aaacgtaccg ggcgcatcag cgtggacttt taccgccgcg
     9781 gctttcgcct gagaaaaact attgatacct tcggccaacg tcgggccgga aaatgctgcg
     9841 ctcagtacca ccgcagcaat agacaaataa cggtacatca cttactccgt gttaaaacga
     9901 gccagcaacg tagcgaaagt tgttttggta tgcaatcagc cagatcagga aagttcctga
     9961 tagttcaggt ggttatttcg caaccagctg ttcaccgcag tgaacacagc gatagatggc
    10021 ctcgccacga actacgcgat tatggcggcg tacggtaagc tgatgttcct ggcacttaca
    10081 gcggtagggg aaggtgttgc gacgcacgga ttgcagttcg aactgatgcg tacgacgggc
    10141 gggaacgccc agcacgcttt ccatcatcca cttccactct ttgccatgtg gcgctacgcg
    10201 gccgaaatgt ttccatacca gcaaatgtgc cagttcgtgc ggtaccactt cttcaataaa
    10261 agcttcactg ttttccaaca gcaaaacggg attgaggcgg atttcatagc tttccagcca
    10321 ggctgttccg gcggaggttc cgcgctgggt gtaagagagt tttggctccg ggtagttacg
    10381 ccctagcttc aggttggcct gggcgagttt ttcccgcagg cggcgcataa cggcctgttg
    10441 gatggcgata gggagacggg atgttttcat agcggcagag gatagagcga agaaaagatg
    10501 actgcaagag gcggcttcct ccgcgacggg aggaagcctg aggagattaa tcgtgagcgc
    10561 cgatttcgcg cagtttacga cctttcatca gattacgttc aatatgttcc agcgaaacgt
    10621 gtttggtttc cggtaccagc cacaatgtca gcaggataaa cagtacgttc agagccgcat
    10681 acacccagaa ggtgttggcg ttacccagcg tgttgagcat ggtcaggaac gttgcgccaa
    10741 cgatcatgtt ggcaatccag ttggtggcgg tggagcaggt gatgccaaaa tcgcggcctt
    10801 tcagcggctg aatttcggag cacagtaccc aaatcagcgg accggcactc atggcaaaac
    10861 cgacaataaa catcagcagc atggcgatgg cgaaatactg cgccgacgga gagtggatac
    10921 cgatatgcat cattgtaccg agtacgccca tgccagcagc catcaccagg aagcccagcg
    10981 ttagcgttgg tttacgtccc cagcggtcaa caaggccgat tgcgataaag gtggcaagta
    11041 cgttggtcag gccgacaatc actgtccccc acatttgctc ggtggtgttg gtataacccg
    11101 ccagttcgaa gattttcggc gcgtaataca tgatgacgtt catcccggtg aattgctgca
    11161 ttacctgtaa cagtacgcca aggaacaccg cgcggcggaa gttgctattc tctttaaaca
    11221 gcgcccagcc actctgttta acctgcaaac tttcacggat ttcatccagt tcgcgtttcg
    11281 cttccgcgct ggtgtcacgc aggcgtagca gcacgcgttc ggcatcaaca aaacggcgtt
    11341 tggcggcaaa ccaacgtggg ctgtctggca ggaagaagac accaatcagc agcaaaattg
    11401 ccgggatgat aatcacaccc agcatccagc gccatgcacc ggtgtagctg aaggcggtat
    11461 cagaaagata agcaccgagg atcccgatag tgatcatcaa ctgatacatc gagatcatac
    11521 tgccgcgaat tttttccggc gcaatttctg acaggtacag tggtgcggta taagaggcca
    11581 cacccaccgc cagccccagc agaacgcgcg aaagaatcag tacttcaacg tttggcgcag
    11641 ccgcagagaa cagtgaaccg gcaacaaaca ggattgcgcc gatcatcagg ctctttttgc
    11701 gcccgagttt aaaggagagc cagccgatgc ccaccgcacc gactgccgca ccgaacatca
    11761 tggagcttac gacccattct tgcgtgtgcg aagttatctg gaattcatct gcaataaacg
    11821 gcagtgcgcc agcaattaca ccgatatcca ggccaaagag taatcccgcc agagcggcaa
    11881 ggaagcagac gaaaaacgtc attgccttgt ttgaccgccc ctgtttttta gcgtcaggca
    11941 tgatgccctc caatatagtt attttttatt gtgaattaag ataggtgagt acgacgtaaa
    12001 aagatgtgaa gcaa
//
LOCUS       DAZSIO010000121        11957 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZSIO010000121 DAZSIO010000000
VERSION     DAZSIO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.121"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(193..933)
                     /gene="cysQ"
                     /locus_tag="ACHX5T_002996"
     CDS             complement(193..933)
                     /gene="cysQ"
                     /locus_tag="ACHX5T_002996"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR8468090.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            1123..3066
                     /gene="cpdB"
                     /locus_tag="ACHX5T_002997"
     CDS             1123..3066
                     /gene="cpdB"
                     /locus_tag="ACHX5T_002997"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR8468091.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(3192..3572)
                     /locus_tag="ACHX5T_002998"
     CDS             complement(3192..3572)
                     /locus_tag="ACHX5T_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR8468092.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            3661..4521
                     /gene="qorB"
                     /locus_tag="ACHX5T_002999"
     CDS             3661..4521
                     /gene="qorB"
                     /locus_tag="ACHX5T_002999"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR8468093.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            4630..5595
                     /locus_tag="ACHX5T_003000"
     CDS             4630..5595
                     /locus_tag="ACHX5T_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR8468094.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            5703..6365
                     /gene="ytfE"
                     /locus_tag="ACHX5T_003001"
     CDS             5703..6365
                     /gene="ytfE"
                     /locus_tag="ACHX5T_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR8468095.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6410..7822)
                     /gene="cycA"
                     /locus_tag="ACHX5T_003002"
                     /gene_synonym="dagA"
     CDS             complement(6410..7822)
                     /gene="cycA"
                     /locus_tag="ACHX5T_003002"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR8468096.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(8130..8750)
                     /gene="fklB"
                     /locus_tag="ACHX5T_003003"
     CDS             complement(8130..8750)
                     /gene="fklB"
                     /locus_tag="ACHX5T_003003"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR8468097.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            8969..9514
                     /locus_tag="ACHX5T_003004"
     CDS             8969..9514
                     /locus_tag="ACHX5T_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HFR8468098.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(9498..10145)
                     /locus_tag="ACHX5T_003005"
     CDS             complement(9498..10145)
                     /locus_tag="ACHX5T_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR8468099.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            10357..11148
                     /locus_tag="ACHX5T_003006"
     CDS             10357..11148
                     /locus_tag="ACHX5T_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFR8468100.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            11158..>11957
                     /locus_tag="ACHX5T_003007"
     CDS             11158..>11957
                     /locus_tag="ACHX5T_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR8468101.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         3032 a         3092 c         2999 g         2834 t
ORIGIN      
        1 tggttatttt atgtcgccat taaatatctc attatagaag taaactatca aaaaacgctc
       61 attattaaaa tgtaacttac tagcaattga atttttgcac tttaaataca atctcttttt
      121 atttataact ttttaggttg ggataaatca ggatggcaga atcaggaaat acactgtttc
      181 tgccatctga atttagtaaa tggacactct gaaccccgga ttcaggaacg actcgcgcgg
      241 agtgtaatcc agcggtttac cctgccagtc gtgaacgtgc gctccggcag ctgcagccac
      301 agcatgtccg gcggcggtgt cccaaatatt cgttggtccg aagcgcgggt acagctgcgc
      361 ctgtccttcc gccaccaggc agaatttcag cgaagagccg atggacgtgg tctgatgttc
      421 gccaagctgt tgcagatact ctttcagctc cgcatctgca tgggaacggc tgataaccac
      481 cagcggcggg cgcgcatcgc ggacctgaat cagcttgcgc acaccgcact cttctttcca
      541 ggccttgcct tctgccgcgc tgtacattac gttcattacc ggcgcataca ccacacctaa
      601 aatcggtttg ccatggtcaa tgagcgcaat gttaacggtg aattcgccat tacgtttaat
      661 aaactcttta gtaccatcca gcgggtctac cagccagtaa cgctgccagt gctgacggac
      721 ttcccaaccg ggaggatctt cttcagaaag gaccggaata tccggtgcca gcgtacgtaa
      781 accgtccatg ataacggtgt gagcggcaat atccgctgcc gttaccggag aattgtccgc
      841 tttgctgacg acgtccatcg gtttcgtccc gtcgtagacc tgcataatgg catcgcctgc
      901 attccgtgca agctggcata cttgatctaa catttctcca cctcgtctct gtgagcggtg
      961 ttaacttatt gttttactta taccctatcg ttaatgaatg cgccaactgt gatagtgtca
     1021 tcattttcaa agcgtaaaat tgtggcattc ttcactgttc tataagtaag gcgtttattc
     1081 tcccttttct ttcgtattcc cgatgataaa aggatgtccc tgatgattaa gtttagcgca
     1141 acgctcctgg ccacgctgat tgccgccagt gtgaatgcag cgacggtcga tctgcgtatc
     1201 atggaaacca ctgatctgca tagcaacatg atggatttcg attattacaa agacaccgcc
     1261 acggaaaaat tcggactggt acgtacggca agcctgatta acgatgcccg caatgaagtg
     1321 aaaaacagcg tactggtcga taacggcgat ttgattcagg ggagtccgct ggccgattac
     1381 atgtcggcga aaggattaaa agcaggtgat gttcatccgg tttataaggc gctgaatacg
     1441 ctggattata cggtcggtac actcggcaat catgaattta actacggtct ggattacctg
     1501 aaaaatgcgc tggcgggagc gaaattccct tatgtaaatg ccaacgtcat tgacgccaga
     1561 accaaacagc caatgtttac accgtattta attaaagata ccgaagtggt cgataaagac
     1621 ggaaaaaaac agacgctgaa gattggctat attggcgtcg tgccgccgca aatcatgggc
     1681 tgggataaag ctaatttatc cggaaaagtg acggtgaatg atattaccga aaccgtgcgc
     1741 aaatacgtgc ctgaaatgcg cgagaaaggt gccgatgtca ttgtcgttct ggcgcattcc
     1801 gggctgtctg ccgatccgta taaagtaatg gcggaaaact cagtttatta cctcagtgaa
     1861 attccgggcg ttaacgccat tatgtttggc catgctcacg ccgttttccc gggtaaagat
     1921 tttgctgata tcgaaggggc tgatatcacc aaaggcacgc tgaatggtgt tccggcggta
     1981 atgccgggca tgtggggcga tcatcttggg gtggtcgact tacaactcag taatgacagc
     2041 ggtaaatggc aggtgacgca ggcgaaagcg gaagcacggc cgatttacga catcgccaat
     2101 aaaaaatccc tcgcggcgga agacagcaag ctggtagaaa cactcaaagc cgatcacgat
     2161 gccacacgcc agttcgtcag caagccaatc ggtaaatctg ccgacaatat gtatagctat
     2221 ctggcgctgg tgcaggacga tccgaccgtg caagtcgtga acaacgcgca aaaagcgtat
     2281 gtcgaacatt acattcaggg cgatccggat ctggcaaaac tgccggtgct ttcagctgcc
     2341 gcaccgttta aagttggtgg tcgcaaaaat gacccggcaa gctatgtgga ggtggaaaaa
     2401 ggccagttga ccttccgtaa tgccgccgat ctttatctct accccaatac gctgattgta
     2461 gtgaaagcca gcggtaaaga ggtgaaagag tggctggagt gctccgccgg acagtttaac
     2521 cagattgctc ctaacagcac gaaaccacag tcactcatca actgggatgg tttccgcact
     2581 tataactttg atgttattga tggtgtgaat tatcagattg atgttaccca acccgcccgc
     2641 tatgacggcg agtgccagat gattaatgcc aatgcagagc ggattaagaa cctgaccttt
     2701 aatggcaagc cgattgatcc gaacgctatg ttcctggttg ccaccaataa ctatcgcgct
     2761 tacggcggca aatttgccgg tacgggcgac agccatatcg cttttgcttc accggatgag
     2821 aaccgctcgg tgctggcagc gtggattgct gatgagtcga aacgtgcggg ggaaattcat
     2881 ccggcggcag ataacaactg gcgtttagca ccgatagctg ccgataagaa actggatatc
     2941 cgtttcgaaa cttccccgtc agataaagcc gcagcgttta ttaaagagaa agggcagtat
     3001 ccgatgaata aagtcgcgac cgatgatatc gggtttgcga tttatcaggt ggatttgagt
     3061 aagtaaaaca cttctttttc ggcctataaa tcaaccgcat ccggcattta ttggcggatg
     3121 cgatgctgcc gtatcttatc caccctacaa gccatgaacc gtagaccgga taagcgcagc
     3181 gcatccggca gttatgccgc acgttcatcc cgcaccgcca acacctcggg caaattcaac
     3241 tcaatccagt ccgccagtgc agcaaccttt tcgctcacct gctcgcccag cggcgtgagg
     3301 ctatattcca catgcggcgg caccaccgga tacgcgatac ggttaagaaa accatcctgt
     3361 tctaacgcct gtaacgactg cgcaagcatc ttttcactca ccccaccgat tttgcgccgc
     3421 aggtcgctaa agcgatgagt accttcgcgt agcgccacca gaatcaacac cccccaacgg
     3481 ctggtgacgt gtttcaacac ctcgcgcgac gggcactgtt ccgcaaagag attaccctct
     3541 ttcagttgtt gtgacaggct aacctgactc atttcatact tacctttttg cacgtactta
     3601 ctaaaagtaa gtttaggtgt tagcgtattt aaacacaaga caaaacgatg gagacttccc
     3661 atgatcgcta ttactggtgc cactggccaa cttggtcact atattattga atccttgatg
     3721 aaaacggttc ctgccagcca aatagtggct atcgttcgta atccggcaaa agcccaggcc
     3781 ctggcagcac aaggcattac cgtgcgtcag gctgactaca gcgatgaagc cgcactgaca
     3841 tctgcacttc agggagtgga aaaactactg ctgatctctt ccagcgaagt gggtcaacgt
     3901 gccccgcagc atcgtaatgt tattaatgcc gcaaaggcgg ctggcgtgaa atttatcgct
     3961 tataccagcc tgctacatgc agatacctcc ccgctcggcc tcgccgatga gcacatcgag
     4021 acggagaaaa tgctggctga ttctggcatc gtttacaccc tgctgcgcaa cggctggtac
     4081 agcgaaaact acctcgccag cgccccggca gcactggaac acggcgtatt tatcggtgcg
     4141 gctggcgatg gcaaaatcgc ctcagcaacg cgggcagatt atgcgacagc tgcggcacgc
     4201 gtgattagcg aagccggtca cgaaggcaag gtttacgaac tggcgggcga tagtgcctgg
     4261 acgttgacac agttagcggc agagctgacc aaacagagcg gcaaacaggt tacctatcaa
     4321 aatctgagcg aagccgattt cgccgcggca ctgaaaagcg tcggactgcc cgacggactg
     4381 gcggatatgc tggcggattc tgacgttggc gcatcgaaag gtggtctgtt tgataacagc
     4441 aaaacgctta gcaaattgat tggctgccca acgacaacgt tagccgaaag cgtaagccat
     4501 ctttttaatg ttaataacta gttaattaaa gtggcatcct cccgcatcct ctctgataat
     4561 gacgggatgc cgggagcaat catgtctgct tcctgaacgt ttcttctgac agaccaatgg
     4621 atgccagtaa tgattagcgg cgtgctgtac gccctgttag cagggttgat gtgggggctt
     4681 atttttgtcg ggccgttgat cgtgccggaa tacccggcga tgttgcagtc gatggggcgt
     4741 tatctggcgt tagggttaat tgcgctaccc attgcctggc tgggacgcgt gcgtctgcgt
     4801 cagttggcgc gtcgggactg gcttaccgcc ttgatgctca ctatgatggg caacctcatt
     4861 tattacttct gccttgccag tgccattcaa cgtactggcg cgcctgtttc cacgatgatt
     4921 atcggcaccc tgccggtggt gattcctgtc tttgccaatc tgctttatag ccagcgcgac
     4981 ggcaaactcg cgtggggaaa actcgccccg gcactgattt gtattggcat cggcctggcg
     5041 agtgtgaata ttgctgagtt aaaccacgga ctccccgatt ttgactgggc acgttatacc
     5101 tctggcatcg tgctagcgtt agtttccgtg gtctgctggg catggtatgc cctgcgcaac
     5161 gcccgctggc tgcgggaaaa tcccgacaaa catccgatga tgtgggcgac ggcgcaggcg
     5221 ctggtcacgc tgccggtttc tctcatcggc tatctcgtcg cctgttactg gctgaatata
     5281 caaacgccgg acttctcctt accttttggc ccccgtccgc tggtgtttat tagtctgatg
     5341 gttgcgatag ccgtgctttg ctcatgggtt ggcgcactct gctggaacgt cgccagccag
     5401 cgattaccga cagtgattct cgggccgctg attgttttcg aaacgctggc aggtttgctg
     5461 tacacctttt tactccgcca gcaaatgccg ccgctaatga cgctgagcgg tatcgcgctg
     5521 ttagtgattg gcgtggtcat cgcggtcaga gcaaaaccag aaaaaccttt aactgaatct
     5581 gtctcagaaa gttgacatgc tggcagtgag ttaaataagc ctctgctacg taagggttat
     5641 agcttttgcc ttaaaggtgt atttaaaata catcttatct tattaagaat gaggtatcag
     5701 ctatggctta tcgcgaccaa cctttaggtg aactggcgct ctctatccct cgcgcttcgg
     5761 ctctgtttcg taaatatgat atggattact gctgtggcgg taagcagacg ctggcgcgcg
     5821 cggcggcacg taaagaactg gatgttgagg tcattgaagc tgaactggca aagcttgctg
     5881 aacaaccgat tgagaaagac tggcgtagcg ccccgctggc agaaatcatt gaccatatca
     5941 tcgtgcgcta ccacgatcgt caccgcgagc agctgccgga gctgatcctg caagcgacta
     6001 aagtcgagcg cgttcacgcc gacaaaccga gcgtgccaaa agggctgaca aaatacctga
     6061 ccatgctgca tgaagagctt tccagccaca tgatgaaaga agagcagatc ctcttcccga
     6121 tgatcaaaca aggcatgggc agccaggcaa tggggccaat cagcgtaatg gaaagcgagc
     6181 acgatgaagc gggcgaactg ctggaagtga tcaaacacac taccaataac gtcacaccgc
     6241 cgccagaagc ctgcaccacc tggaaagcga tgtataacgg cattaatgaa ctgattgatg
     6301 acctgatgga tcacatcagt ctggaaaaca atgtactgtt cccacgcgcc ctagcgggtg
     6361 agtgatctaa aagctggatg gcattgcgcc atccagcatg ataatgcggt tatttccgca
     6421 gttcagcagc ccgcttctta ccaataaaca gccagcccaa ccccagcgcg ataaaccaca
     6481 gcggggtaac cagcagcgcc tggcgagtgt catcttccag tgtcagcaac acgaccacga
     6541 acacaaagaa cgccatacat acccagcaca tcagcttgcc gagcggcatc ttgtagatcg
     6601 acttttcatg cagatgagga cgctgtttgc ggtacaccag gtaagagcaa aggataatcg
     6661 tccagacgaa cataaacaga atcgcggaaa cggttgtaat catcgtgaac gcgccaatca
     6721 cgctaggatt cacatacaac atcaccacgc cgccgagcag acagatacac gagaaggtca
     6781 accctttcgc gggtactgcg cgcttagaaa gtttagcgaa cgctttcggc gcaacacctt
     6841 cctgcgccag accaaacagc atacggctgg tagagaagac accgctgtta gcggaagacg
     6901 ccgcagaggt cagcaccaca aagttgatca cgctggcagc cgcaggcaaa cctaccaaca
     6961 caaacagctc aacgaacggg cttttctccg ggactaccga actccacggt gtcacggaca
     7021 taatcacaat cagcgcgaag acgtagaaca taatgatacg gatcggaatg gagttaatcg
     7081 cgcgtggcag tgatttctcc ggatctttgg tttccgcagc tgttgtacct accagctcaa
     7141 tccccacgaa agcgaaaacc gctatctgga atccggcaaa gaagccactt aaacctttcg
     7201 ggaaccagcc gccgtcattc cacaaatgcg cgaatgacgc ttccacaccg gtcggtgact
     7261 gaaagtgcat cgccaccatg accaggccga cgacaatcag cgacacgatg gcgacgattt
     7321 tgatcatcgc aaaccagaac tccatctcac cgaacatttt cacggtggca aggttgagcg
     7381 tcagcagcag cactatcacc gccagcgagg cgacccagtc ggagagatcg gggaaccaga
     7441 actgagcata agccgtgatc gccaccacgt ctgccatacc ggttacaacc cagcagaacc
     7501 agtaagtcca gccggtgaaa tatcccgccc acggccccag taaatcggaa gcgaagtcac
     7561 taaaagattt gtattccaga ttcgaaagca gcaattcccc cattgcccgc atcacgaaaa
     7621 agagcataaa accaatgatc atataaacga aaatgatcga cggcccggca aggctaatcg
     7681 ttttgccgga ccccataaac aacccagtac caatggcacc tccaatggca ataagctgaa
     7741 catgtcggtt tgtgagattg cgccgtagcg actgttcagc cggagcctga tcatcggcaa
     7801 cgacttttac ctgatctacc atgttttttc ttcctgtacc tgtctgtgtt gttcaggctc
     7861 tacggccttt agtcagtcta tgatacgacg aatctgtatc gggactcatc gatattaggt
     7921 aacaatacgc gggatgaata ctaagattta gatctaatgt taattttatg tttaaagtga
     7981 gtgtcatatc acggtgataa cgcgaaacag ctcacaaaaa tacacgctta cggggtcttt
     8041 gcaacacaaa atagcgccgt ttggcaaact gaagggttta ttgctgaatg cctgctcccc
     8101 tctggttcga ggggaacaga atgcgctgct tagaggattt ccagcagttc gacttcaaac
     8161 accagggtgc tgaacggagg gatggatgca cctgcgccgc gctcgccata tgccagttcc
     8221 tgcgggatag tcagttccca tttagaacct accggcatca gagtcagtgc ttcaatccag
     8281 ccagggatca cgccattaac cgggaattca gcgggttcac cacgagcaac ggagctgtca
     8341 aacacggtgc cgtcgatcag tttaccggtg taatgaacac gaacgcggtc ggtacgtgcc
     8401 ggaattgcgc cttcaccctg gttgatcacg cggaattgca ggccagattc ggtgctattc
     8461 acaccttctt ttttggcgtt ttcttccagg tatttcacgc cttcagcagc catcgcctgg
     8521 aaacgctgac gacgaacggc atcggcgcgt tcgtggattt cacgcagcgc gcgatgcacc
     8581 acatcaaccg gcacagccgg atgtttgcct tccagcgcat cggcaatacc tgcaaccagt
     8641 gcttctggca gcagcccttc caggccagat tcactcagtt gttgccctac ctgcaaacca
     8701 atgccgtagc ttgcttgcgc ttcgatggtg tcaaaagttg gggtggtcat cgtatttcct
     8761 ttcttagggt ataaaagtag cgggcagcat atcagccaca cttctttggg taaaactttg
     8821 tcgcaggaat gatgacaaat cccggggaaa aagaaacaat agcaatatcc cgtatgaata
     8881 taataaaggc gggtttacca cggacatcat agcgttagct tattcatagg ctatgattga
     8941 ggaacaagac gtggagcagg aggaaaccat gcccgggcgc tttgaactaa aaccaaccct
     9001 ggagaaagtc tggcacgcgc cggacaattt tcgcttcatg gacccgctgc cgccgatgca
     9061 tcgtcggggg atcattattg ccgccattgt attggtggtc ggatttctgc ttccatctga
     9121 tgatacgccc aacgcgtcgg tggtgacgcg tgaagcgcag ttagatattc agtcgcaatc
     9181 acaaccgccg acagaagaac agccgcaaac tacccaaacg caaccattcc agccagatag
     9241 cggaattgat aatcaatggc gctcttaccg tgtcgaaccc ggtaaaacga tggcgcagct
     9301 gttccgcgac cacggactgc ccgctaccga tgtctatgca atggcgcagg tagaaggcgc
     9361 gggtaagccg ctgagtaatt tgcaaaatgg gcagatggtg aaaatccgcc agaacgccag
     9421 cggcgtggtg acgggcttaa ccattgatac ggggaataat caacaggtcc tgtttacccg
     9481 ccagccggat ggcagtttta ttcgtgcgcg gtagatgaag ggtcaaccaa aagccagcta
     9541 aggattgtct ctttgatgac cttacgtctg gcttccagtg cctgggggga aaccatactg
     9601 atatgataaa ctaggccaag cgtatgctgg ttgatcaaat aatatccccc aagcgcagca
     9661 atattaatat tcacctgcaa tgggtcgata cctggcttaa aaatattgta tttcttccct
     9721 tcatccaata aggattccat taactgcaag tgagcatggt taatttctaa gagacgctgt
     9781 gattttgcat aatgcacacc tttactttga ttttcactat gcacaatttt taaaaaccaa
     9841 ggattagcaa tataataatc ccatgtaaaa tcaataattt tctcaatggc tttttgtggc
     9901 ggaagatctg agaaatcaag ggccttttct ttttgtcgaa tatcgttcca ggtatattcc
     9961 agcacttcga taaataaatt ttctttatta ccaaagtggt gataaaccgt ctgtttacta
    10021 caccctgctg cctttacaat attatctact ctggcccctt cataaccata ttcggcaaat
    10081 tcattaaccg cacatagcag caatttttcc ttcaggctct gtacgttgct ggtatcaata
    10141 tacataacgc ttccccacga cctcttcgtg gtgactatca taacctgatt tgatttcagc
    10201 ttcagaaaaa agggcaaaaa cttgtacttt catcaggtta tcaaatgtcc tgtgatcgcc
    10261 atcgcatatc aaccaactag atggtttatc tttttatcct ccagtcgcaa gctaatcatc
    10321 accaaggaaa caaaattcaa aaaagagagc tattgtatga aatccactcg tcctgttgcc
    10381 gtgattaccg gagccactcg cggtattgga aagggctgtg cgcaggagct tgctcgtggc
    10441 gggtttaacc tgctgattaa cgatcgtccc gatgccgaca gcgtagaaaa actgcatatc
    10501 acacaacaag aatgtttggc ggaaggcgtg gaagtgattt gcttccctgc tgatgtcggt
    10561 gatctatctc tgcatgagga gatgctcgac gcggcgcaaa accagtgggg gcggctggac
    10621 tgtctgctca ataacgcggg gatttcagtg aaaaaacgcg gtgacctcct cgacctcgaa
    10681 ccagacagct tcgatcaaaa cattgccatt aacacccgcg cgccgttctt ccttgctcag
    10741 gcattcagta agcgcttact ggcacagccg aaaccggaag cggagctgcc gcaccgctcg
    10801 attattttcg tcagttccat caacgccatc atgctggcga tgaatcgcgg cgaatacacc
    10861 attgccaaaa ccgccgtttc tgccgctgct cgtctgtttg ctgctcgcct ttgtaatgaa
    10921 caaattggcg tttatgaagt gcgccctggt ctcatcaaaa ctgatatgac tattccggca
    10981 acggcttatt acgacgaact gattgccaaa ggtctggtgc cgtggggacg ttggggttat
    11041 ccggctgata ttgcttccac cgtccgcgcg atggcggaag gtaaactgat ttacacctgt
    11101 ggtcaggcgg tcgcaatcga cggcggctta agtatgccgc gcttctgagg ctgaatcatg
    11161 agagatctgt tacaacgccc ggatttattc agtatcaata ccgcgacact gggctataaa
    11221 acgccgctac ccgccattat tgatgcctgc gccgcacgcg gtattggcgc aattgccccc
    11281 tggcgcaggg agttgcaggg tgaagatctc cagcaaatca cccgccagct tgccgccagt
    11341 aacatgagcg tttccggttt gtgccgtagc acttactaca ccgcgccgac actggcggaa
    11401 cgcaaactgg cgattgatga taaccgccgg gcgctggacg acgcagcggt gcttaacgcc
    11461 gcctgttata tgcaggtggt tggcggtctg ccaacgggca ctaaagatct atatgaagcg
    11521 cgagaacaag tgaaacaagg gattcgccag ttgctgccac attcgaaaga tgtcggcgtg
    11581 ccaattgcgc tggagccact gcacccgatg acagctgccg atcgctcttg cctgtgcacg
    11641 ttacgtcagg cgctggactg gtgtgatgaa ctcgatcccg atggtgagtt tggcctcggc
    11701 gtggcggtgg atgtttatca cgtctggtgg gatccggatc tcgccagtca gatcctgcgt
    11761 gccggaaaac gcatcctcgc gtttcatgtt tccgactggt taatgccgac cactgatctg
    11821 gtcaatgacc gtggtatgcc gggagatggc gtcatcaata ttccgtcaat tcgtcggctg
    11881 gttgaaaacg ccggatttaa tggtgctatt gaactggaaa tattctcgcc ttactggtgg
    11941 cagaaagata ttaacag
//
LOCUS       DAZSIO010000122        11912 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZSIO010000122 DAZSIO010000000
VERSION     DAZSIO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11912
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.122"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..2251)
                     /locus_tag="ACHX5T_003008"
     CDS             complement(<1..2251)
                     /locus_tag="ACHX5T_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HFR8468102.1"
                     /translation="MGTGLAKADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTEGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFRDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTLYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVE"
     gene            complement(2275..2898)
                     /locus_tag="ACHX5T_003009"
     CDS             complement(2275..2898)
                     /locus_tag="ACHX5T_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFR8468103.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2895..4583)
                     /locus_tag="ACHX5T_003010"
                     /pseudo
     CDS             complement(2895..4583)
                     /locus_tag="ACHX5T_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="ACHX5T_003011"
     CDS             complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="ACHX5T_003011"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR8468104.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7506..8228
                     /gene="ubiG"
                     /locus_tag="ACHX5T_003012"
     CDS             7506..8228
                     /gene="ubiG"
                     /locus_tag="ACHX5T_003012"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR8468105.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8356..11912)
                     /gene="yfaL"
                     /locus_tag="ACHX5T_003013"
                     /pseudo
     CDS             complement(8356..11912)
                     /gene="yfaL"
                     /locus_tag="ACHX5T_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2754 a         3302 c         2943 g         2913 t
ORIGIN      
        1 attcgacatt gaccagttca ccaggctggt aatgggtttt gtccgttttc acccggatat
       61 caagctgagg aacggcaact ttgatcccgg cgttctgaaa actgtactga ccattacggg
      121 tatacagcac cgaaaaagtg atgttaggcg caaaggaatt gctcactggt acccgggctt
      181 catacagggt atcgtttaaa cgttgtagcg ttagccagtt tgccggatgc gaaagcagcg
      241 actgctgttc cacgcgatcg cgttccagcg tcaataacgc ttcatcaatt ggctccggga
      301 aagtaatcag catcttcgcg gtttcgcctg gctggtacag cgttttatcc gccacgatat
      361 ctaccgtacc agtatgcgcc gtgctgccct taccgctgac ggcatgactt aacccagcga
      421 gaattaagcc gtctttatcg cgtaatgtca gattgtagtt gccaggttta gcgaaattga
      481 cggtaaagga tttgccgcct gacggtagct ctccgctatg gctcgtgcgg tcttcgagac
      541 gcaaccattc atacgtaaca ggaacctgtt ttgaagattc cagcgcggca taacggaaca
      601 caaccgactc gccgctatta ctgtattgtg cggcggtact taaagagtaa tgcgccaggc
      661 cgcgttcaat gaggatctct ttggtggtgg tgacgcgata cgccgcgccg tcactggcgg
      721 agacggttaa caaatagcgg ctcggtttat cggcggcggg gagatttaac gccacatgac
      781 cgctggcgtc ggacaccgtt tcgctgcctt ccagcgacac ggggaaacgt ccggcataac
      841 gcaaatcgtt accgaccatt gataattgct gagcgcgcaa acttaactgc acgcgggcat
      901 tttttaccgg ctcgccatcc gggtagagca gttgcagttt gccgctgacc gcttcgccag
      961 ttttgaactc ttttttgtcg agagctaaac caatctcgaa atgtggcttg atgtagtttg
     1021 ccacgcgaaa actgctgcta tagacctgat tgcggtaagc aagacgtaac tcataacctc
     1081 cggcaacggc attttctggc aggcggaaac ttccctgccc gccattgcgc gcatccagcg
     1141 tgacatcgac ggtttgcaac agactgccgt tagcgtccag caccgaaagc ttcgccgggg
     1201 cgctgacgat gggggatgaa tgcaacggat cgcggaactc gcggcccatc actttaacat
     1261 cgacacgatc gcctgcgcga tatagcgggc gatcggtaaa aatatacaag cgggtgttgt
     1321 agatttcgct ttcgtagaag aagttctcgg agacaaaaac gccgccttca gcatccttac
     1381 ccagaatgta tgaacgttct ggcgatatat gttgtaactg caaagtaccg ctgtcatcgg
     1441 tcaccccgcg ggtcatcacg ccaagacctt cagtccacaa gatctctgag ccgggcttcg
     1501 cttcaccctg ttttttaccc gcggtccata ccagaagctc gttacctgac actttgctaa
     1561 gcgccacggt atcggaaaca aacaccaccg tcgtcgcccg atacccacct accatcgcct
     1621 cgacgaggta cagccccggc tcttgttggc cgagaggaat ataaatatta cccggctgcg
     1681 gcgagatgaa attgctggat gcgccttcca gttttacccc ttgctgcggc tcgaccggtt
     1741 tagcctgcca tagtggataa cggaactgtt ccaccaaggg atattttttc agcggggaaa
     1801 actggttgtt ctgtacataa cgggaaggtt taataatggc attgccgaga tgtaattcgg
     1861 gtaatgcctg agtcacattc tgccgtgact gagaagagaa agtacgctgc atcacgcggc
     1921 gagatttgcc gtaccagtta tcccacagcc aggtcagcgt attgttcagc ccgtcgccca
     1981 gatattgcgg ttgcaccaca atgcgatgca ggtttttctg ctggcgcaaa aatgccatcg
     2041 ggtcaggaat acgatatagg cgaacgtcca cgccgccgta ctcttccatc tgatagcgcc
     2101 gataatcacg ccccggcgct tccagtcgca ctttcgcctc ttcactgctg ctaaaactgc
     2161 tgtcagcaag caaaaagaat gttcccccgg cgggcggcgc atagttactg gaaggaagcg
     2221 aatcatcagc tttagcaagc cctgttccca caagagacag ggacaagcac gctgctatcg
     2281 cgccagaaaa tttaaacgat aaatgccaat gaaattggga ttggaatcgt tgggtatcca
     2341 tcgggtgtcc ttccatgtca taagttgttg cagactgact gcgcgcattc cgttgtcagt
     2401 tttcgtggcg cttccggtgt ggtagatgac gtaacgcccc atccagacca ttaagtgctg
     2461 ggcatcgccc tgatcgaaaa aaatcatatc gccaggcagc gcctggttta tgtcttggcc
     2521 aataaactgg ctgttgtact gaatcaaatt aatcgcggtc acgtaggggc cgtttttccc
     2581 gctcccctga ttccagtttt gcgccagttg acgctgttca ggtgttagcg tcatctctgg
     2641 cggcaaatac tggctagata aaccgttact ttttagccat ttactgtcgt gaactttcag
     2701 cgtctcgttc gccgcaaatc gcaccaggcc cgcacaatcc tgctgatacc agcgtggact
     2761 tggcccctgg cggagctgtt cttgtgcaat gcgtacaaac caggcgcgaa acagcctgga
     2821 ttgttcgaca ttcagcattt cgctatgggc aacaacacaa cacagccagc aaatcagcgc
     2881 cagcagcccg tgtctcatag cggctgccag gttatgggta gccactgcca ggcgacaccg
     2941 ggttccatct gggccagctt catgacataa cgcggttgtt gagataaagc gtccagcttc
     3001 ggcattaata aagtttgtgc ggcgttataa aaaaccggtt cgagattctt cggcagactg
     3061 gtcaacgttt cgttacgcag cagtttcgcc acgccttgtg gattgatata gagcggaacg
     3121 atgccatcag tgggtattac atccaccatt gccgggcggg ttttattcag tgtttgcagg
     3181 gcgttattaa ccagcgtgtc atcgagggag aaaagcagcg ttttgttttg catcgccagc
     3241 gacacacgga aaaaataatc cgacattaat tgatcgggtt gcgccgcctg cgctttcgga
     3301 tactgaccgt atcgggaact cacttcgcga caccagattt gcgcttcgcc ctgctgagtc
     3361 tggcttaccg gcaaaacacc ttctggcgct ttgctttcgt gcgcaccaat attttgcgta
     3421 aacagtttcc ctggcaattg cgcctgttcg gcagtgccat caaactgacc gacaaacagc
     3481 ggggtttgca attttgagtc ttcataccag cacagccccg cggcaccgtc taacgcccca
     3541 ttcagcttgt cgttttcctg gctgatgtgc gaaagcatct cttcggcaat accgtgtgaa
     3601 tacggcaccg ccacacagaa gctggctccg gcaggcatac tgttccagac cggcgtaaaa
     3661 tcgaaactgg catctacgct ggcggattca tcatttaacg ccacaaaact gtgccagccg
     3721 tcgttaccca tttcgaagcg tacgccagca aaagaaggca ttaaccgctg gtagccaaaa
     3781 cccagcaacc tggcgctgac cacgatgcgc aggcgtactg gcgttttttc ggctgtacgc
     3841 tcttccaggc caaagctggc ttgccagcgt tttttaccgc tcaacaaatc acttgcgatc
     3901 gccgtggctt cggtatcctg ctgatcatct ttaaacaaca tatccgtact ggaaaacacc
     3961 agcatcttgt cctgatatgt cgcgaacatc agggcgttat tgccgttata gcgcaactga
     4021 taaacaggaa tagtttcact atttatcttg atgctactga tttccgtttt gcttaactag
     4081 ctgtcgctgg tagcggcaaa caacaatggc tccagcagtt tgcttaaacc actgcgctgg
     4141 atcagcacca tgtaatgtga aagatggcct tgtttatcgt gccatagcgc cgcctgcgcg
     4201 ggctgatcta agagtgacga aaagagctta tctttcagcg tgagatcgtg ttcatagaca
     4261 atgcggcgaa tgctgccttc aatgcccaga cgatcggcat gattctgata atagaaaacg
     4321 aaatcttcgc tcagaacatc gtggagaaac ggaatggtga ggagatcttt gggaagctgg
     4381 ctcagagagt cgctgtctag aaagaggtcc ggctcattga gatcgatttg cagattgttg
     4441 tgcaccacca gcggcgacaa cgttttttct ggcccactgc cagcatattg caacgcccag
     4501 acgccagcgg aaagcagtgc tattgcgcca aaacctacaa gaccatagaa ccgccagcct
     4561 ttcgcctttt tttcaccact cattgccaca ttccttgtgt atagccagcc attttttacg
     4621 ggcacagcca aactttaccg cgccctaata cgacaaaagc ccagactttg cagcctggac
     4681 ttttcaattc aaacaaggga gatagctccc ttttggcatg aagaagtaaa attattcttc
     4741 ttctggctcg tcgtcaacgt ccacttccgg agcaatttca tcatcccctt ccgcggcact
     4801 gccgtcgatg gtatccagat cttcctcgtc aaccggttca gcaacacgct gcagacccac
     4861 tacgttttca tcttccgcag tacggatgag gatcacgccc tgggtgttac ggcccacgat
     4921 gctgatttct gaaacgcgag tacgtaccag cgtaccggca tcggtgatca tcatgatctg
     4981 gtcgcagtca tctacctgca ccgcgccaac cactaaaccg ttacgttcgg taaccttgat
     5041 ggagataacc cctttcgtcg cacgcgactt ggttgggtat tccgccactg cagtacgttt
     5101 accgtaaccg ttttgcgttg cggtgaggat tgcgccatcg ccacgaggca cgatcagaga
     5161 gacgacttta tcgccttcac ctaagcgaat accgcgcaca ccagtggtat tgcagcccat
     5221 cgcacggaca gaagactctt taaagcgcac cactttacct tcggcggaga acagcattac
     5281 ttcatcttcg ccactggtca gatcaacgcc gatcagctca tcgccgtcaa ccagtttgat
     5341 cgccacttta ccggcggtac gcagacggtt gaattcggtg aggacggttt tcttcacagt
     5401 accgttagcg gtcgccatga agactttcac gccttcttca aactcggtca ccggcaggat
     5461 cgcggtgata cgttcgtcct gttccagcgg tagcaggttg acgatcggac gaccgcgcgc
     5521 gccacgagtg gcttccggca actggtaaac tttcatcgaa tagacgcgac cacggctgga
     5581 gaagcacaga atatggtcgt gcgtgttcgc caccagcagt cggtcgataa agtcttcttc
     5641 tttaatacgt gcggcagatt tacctttccc gccacgacgc tgcgcttcgt attcagaaag
     5701 cggctgatat ttaacgtagc cctggtgaga gagcgtcacg accacatctt cctgggtgat
     5761 cagatcttcc aggttgatgt ctgcgctgtt ggcggtgatt tcagtacgac gtttgtcacc
     5821 gaactgttca cgaaccagct ccagctcttc gcggatcact tccatcagac gatcggcgct
     5881 accaagaata cgcaacagtt ccgcgatctg atccagcagc tctttgtatt cgtcgagcag
     5941 tttttcgtgc tcaaggccgg tcagtttctg caaacgcaga tccagaatcg cctgagcttg
     6001 ctgttcggtc aggtagtaca gaccatcacg cacgccgaac tctggctcca gccattccgg
     6061 acgcgcagca tcgtcgccag cacgttccaa catggcggca acgttgccca gctgccacgg
     6121 attagcaacc agcgcagttt tcgcttctgc aggcgtcggc gcatgacgga tcagttcgat
     6181 gatcgggtca atgttcgcca gcgccacggc taatgcttca aggatatgag cacgatcgcg
     6241 agctttacgc agttcgaaaa tagtacgacg ggtcaccact tcacggcggt gacgaacaaa
     6301 cgccgcgatg atgtctttca ggttcatgat cttcggctga ccatggtgca atgccaccat
     6361 gttgataccg aaagaaacct gcaactgggt ctgggagtag aggttgttga gcacaacttc
     6421 accgaccgca tcgcgtttca cttcaatcac gatgcgcata ccgtctttgt cagactcgtc
     6481 acgcagcgcg ctgatgcctt ccacgcgttt ttcttttacc agttccgcaa tcttctcgat
     6541 caggcgcgct ttgtttacct gatacggaat ttcgtggacg ataatggttt cacgtccggt
     6601 tttggcgtca acttccactt cagcacgggc gcggatatat accttgccgc gaccggtacg
     6661 gtaagcttct tcaataccgc gacgaccgtt aatgattgcc gccgtcggga agtccggccc
     6721 cgggatgtgt tccatcagcc cttcaatgct gatgtcttca tcatcgatat acgccagaca
     6781 accgttgatg acttccgtca ggttgtgcgg cgggatgttg gttgccatac ctacggcgat
     6841 accggaagaa ccgttcacca gcaggttagg aattttggtt ggcatgacgt cgggaatttt
     6901 ttccgtgccg tcatagttat caacgaaatc gaccgtctct ttttcgagat cggccatcag
     6961 ttcatgggca attttcgcca gacggatttc cgtataacgc attgccgccg cagagtcgcc
     7021 gtcgatggaa ccgaagttac cctgaccgtc taccagcatg taacgcagcg agaatggctg
     7081 cgccatacgg acgatcgtgt cataaaccgc cgagtcacca tggggatggt atttaccgat
     7141 tacgtcacca acgacacggg cagatttttt ataggctttg ttccagtcat tgcctagtac
     7201 gttcatggcg taaagtacgc gacggtgtac cggcttcagg ccatctcgga catctggcag
     7261 cgcacggcca acaatgaccg acatcgcata atccagatag gagctcttta gctcttcctc
     7321 aatgttgacc ggtgtaattt ctctcgcaag gtcgctcatc taaccgctat ccctctactg
     7381 tatcccggat tcaaaggtcg caaattataa cacagccgcg cagtttgagg taaacctata
     7441 cgctttattc acatccaatg cctgatatac tcgtttgtct tgccaattac ggagtagaag
     7501 tgccaatgaa tgccgaaaaa tcgccggaaa accataacgt agaccacgaa gagatcgcta
     7561 aatttgaagc cgtcgcctcc cgctggtggg atcttgaagg tgagttcaaa ccgttgcacc
     7621 gcattaaccc gctgcgtctg ggctatattg ccgagcgtgc tggcggttta tttggcaaaa
     7681 aggtgctcga tgtcggttgt ggcggcggca ttctggccga gagtatggcg cgcgaaggcg
     7741 cgacggtgac cggtctggat atgggctttg agccattgca ggtggcaaag ctgcacgcgc
     7801 tggaaagcgg cattcaggtg gattacgtgc aggaaaccgt ggaagagcac gcggcaaaac
     7861 atgccgggca gtatgatgtg gtgacctgca tggagatgct ggagcacgta cccgatccgc
     7921 agtcagtggt cagagcctgt gcgcaactgg tgaaaccagg cggcgatgtc tttttctcga
     7981 cgcttaaccg caacggcaag tcatggctga tggcggtggt tggcgcggaa tatattttgc
     8041 gcatggtgcc caaaggtacg catgatgtga agaagtttat taaaccggca gagctgcttg
     8101 gctgggtgga tcagaccagt ttaaaagagc gacatataac cgggctacat tacaacccga
     8161 tcactaattc ctttaagctc ggccccggcg tggatgtgaa ctatatgctg cacacgcaga
     8221 ataagtgagg ttgatgtttg gccgcgccaa tgcctgatgc gacgcttgca gcgtcttatc
     8281 aggcctacaa atgctctccg taggccagat aaggcgttta cgccgcatcc ggcaattgtg
     8341 atggtcagac agcgattacc atttcaccgt catcgacaaa aaccctgccg tctgggcaaa
     8401 atcatcactc cctttctgcc acgccacgct gccgcgcagg gacactcgct gactgatatt
     8461 gcccgtgact cccactttta tttcaccccg tagcttcacc gcatcgtcac tgatagtgct
     8521 gccatcttct tcaatttccg tcgcatgggg atcgtgatag taattcagat ctaatgttgg
     8581 tgtgacacca accgcggtac gccattcgct gtgtaaaccc agccgcgtct gaatatcatc
     8641 cccctgcgat tgtgacacgc gcgcatggtt agcggcggta aaatcatcct gctgcacgcc
     8701 ctgataaatc acctgcgcct gcggttcaat caccacacca cgccccggta accactgata
     8761 cccagcttcc agcgaggcga taatccccga cgaatggtaa cgatccacgc catcttcatg
     8821 ttcggaaaca tcattgttaa accacgcgta ttgcagccag gccccctgct tctgattacc
     8881 gtgctgatac cagcttgagg tcagcccaac ggcataaccg tggttctggt tatcggcgcg
     8941 agttccggtc atattcgagc ggctgtcgcc ctgattatca ctgtagccac caaccgcccc
     9001 aagcatccac tcgccatccg tgccccagcg cccgctaaac aagccgccgc taagctgcac
     9061 cgtagaagtg tcttcctgct gagccagttg ccctgccgcc gtgtaatgat aacgtccgcc
     9121 gataacacgt aaattcagcg tctgaccatc gccaccagcg tgatcgcgtc gctccatcat
     9181 aaacgcctga tttgccgccc gcaggttatt gagataaccg ccaactttgg cattcagcac
     9241 cggctggtaa gcaggcgtag gggccgggtc gggggttggg tcaggatccg gcgtgggatc
     9301 aggatcggga gtcgggtctg gctcaggtgg cgatggcggc gttacttctt gcgatcgcag
     9361 ataccagtcg ttgttatctt ccactagcgt gtagtcatac gctcccatgt tgacgtagcc
     9421 actgccagcc agactgaact gtgcattgtt ttgaaactgc gtgggatcag ctgcgaaatc
     9481 aaccacttta atgcctgtcg atgtcggctc accaatccct gtaatggagt taaccaccac
     9541 agttgtgttg ccagcagtat taccgttcat caccaattga tcgcttaccg agtcatcgcc
     9601 gtttaattcg ctgtcgaata gtagcgtacc gccgccggta taatcaccgt ttaccgtcag
     9661 cgtatcgcca gcgacgccat tttgcaggct aaccgcaccg gacgtattgg tcagatcacc
     9721 attaaccgtc acgtcagcaa gataggtcag gctgtcagtt tcaaggttgg tattaagctc
     9781 gcctgtcaac gtcacgccat cggtagcatt taccagcgtc ccatcgccgt taaatagtga
     9841 ggcattaagg gcgacggaag tatcctgccc ggtcagacgc aaaaccgtac catcgctgat
     9901 gtcgatagtg ccgctggaag tagcatcaat tgactgaata tcctgatccg cgccagtaac
     9961 gaacgttgcc ccatcaccaa cccacagtga cgaagcataa ggaaagatat ccgcaacatc
    10021 gcctttcagg gtgccttctt cgacacgtac cgccgactgc gttgtaccgc tggcggtcag
    10081 ctccagcgta cccgcccccg ttttagtaaa tgtgctaccc tcatcctgat gctgcccgct
    10141 gctgtcagcc atcagtgcgc cccactgcgt gtctaccccc gcatcaactg ccacttcacc
    10201 gtcggcgcgc atttcaatat cacgcccatg ctcagtcgtc gtagagcgag tatcgacatt
    10261 ggcagtaatc tccatcacgc tttcttgtta cttatcggta aagataacct ggcggttata
    10321 gtgggtatct ccaagctgcg agttgtcgct caccatcagc gtaccggagg cgatttgtgt
    10381 tgtgcccaaa taactattat tgtttgccag acttacctgc ccgtcgctcg catcgaccac
    10441 cacgtagacg ccatctttcc cgtcgccgcc gatatgcaaa ttgtcgccac ctgcaagatc
    10501 taccacatcc tgactgccga taaccgttcc gctgctgcca ctgacttcaa ggccatcatt
    10561 gtatgatgtg ccgctctgcc aagtggagaa atcagagaga tcgagcacac cgccgtttaa
    10621 cacgatcgac tgcggatcgt cctggagagc ggtaagatct gccgcgtcgc cttccagcga
    10681 aagcaccgca ccatcatcaa cgactatatc gccggttagc gccatcgact gcgcccctgc
    10741 cagcacgtag ctgccgtttt gcgcaatggt gagctgacca gcaccttcga tgatgccagc
    10801 aaaactgccc tgattaacag taacgttgcc accagcatcg atatttaaag tgccattctg
    10861 aaagcccgtc aatgcgtgca caaaagtttg ttgggtcgag ccaacgttta gctcagcctg
    10921 attctgatac tgatcaatac tccctatcgt cagaccgtag cagtcttgcg gatcgtcctg
    10981 gcaatgcgta tcgccgacat tcatcaggga gttgctgcga cccagggtaa cttcaccgtt
    11041 ttcaatctgc atctcaccag taaagtcatt gttatctgca ttaagtacca gatcgccgga
    11101 acctgttttg gtgattaacc cggtaccagc aatagagtca acagctccgt cattctctgt
    11161 attgccgata accagcgttt ttccgtcggc aatatcaaag gtaacttcac ttaagccgag
    11221 atacataaag ccacccgccg cagtggaggg accatctcca tagcctgctg cgctattgtt
    11281 ctcatcgact aacacgccgc cgttctggct gtagctgtca tcaacagaaa tatcaataag
    11341 atagggagcc gtcgcgctat tggtatatat cgccccgcca taaccttcgg ctgtgttatt
    11401 tgtaaaggcg gtgttattta ttatcgtata acctgaagga tgcttgctgt cgctattatt
    11461 atcggtaaca tcgattgccc cgccatcgcc atcactgtaa cttgttgatg tatatgcctg
    11521 gttattgtta aaaacatcat cacttaaata gacgtcatta ttaatggtat aaattgcgcc
    11581 acctttgcca tcattagcga tattattgat ggcgttagtg acacgtaaat cgacggcacc
    11641 agtatcatta gtaccagaag aatagattgc gccaccatag ccgcctgcga cgttaccgga
    11701 aaaaataaca tcagtaagat tcagcgttga gttttcttta gcaaatatgg ccccgccgtt
    11761 attatattct cctgtaacgg tgttattggc aaacagagtc attccagttt cattttctgg
    11821 taatagtgaa aattccgctc cttgttgcaa aaatacggcc ccaccgctgg cattattggt
    11881 catatccgaa aaaaccagcc attgcccatc gg
//
LOCUS       DAZSIO010000123        11793 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZSIO010000123 DAZSIO010000000
VERSION     DAZSIO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.123"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            38..778
                     /locus_tag="ACHX5T_003014"
     CDS             38..778
                     /locus_tag="ACHX5T_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR8468106.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            837..1223
                     /locus_tag="ACHX5T_003015"
     CDS             837..1223
                     /locus_tag="ACHX5T_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR8468107.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1232..1561
                     /locus_tag="ACHX5T_003016"
     CDS             1232..1561
                     /locus_tag="ACHX5T_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR8468108.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1533..4574
                     /locus_tag="ACHX5T_003017"
     CDS             1533..4574
                     /locus_tag="ACHX5T_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR8468109.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4574..4903
                     /locus_tag="ACHX5T_003018"
     CDS             4574..4903
                     /locus_tag="ACHX5T_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR8468110.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            4903..5601
                     /locus_tag="ACHX5T_003019"
     CDS             4903..5601
                     /locus_tag="ACHX5T_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR8468111.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5606..6349
                     /locus_tag="ACHX5T_003020"
     CDS             5606..6349
                     /locus_tag="ACHX5T_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR8468112.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6313..6888
                     /locus_tag="ACHX5T_003021"
     CDS             6313..6888
                     /locus_tag="ACHX5T_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR8468113.1"
                     /translation="MQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSDGWYQVRIAG
                     RDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVAIWMPGVGIM
                     ASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMVAQGNVLPVL
                     YGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            6949..10428
                     /locus_tag="ACHX5T_003022"
     CDS             6949..10428
                     /locus_tag="ACHX5T_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR8468114.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10496..11095
                     /locus_tag="ACHX5T_003023"
     CDS             10496..11095
                     /locus_tag="ACHX5T_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR8468115.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11159..>11793
                     /locus_tag="ACHX5T_003024"
     CDS             11159..>11793
                     /locus_tag="ACHX5T_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR8468116.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLS"
BASE COUNT         2774 a         2795 c         3790 g         2434 t
ORIGIN      
        1 acgtaccaga tcacgtacac gaattaagga ggtggtaatg accacaccaa atccactggc
       61 aaagacgaaa ggtgcgggga cgacgttctg gatgtatacc ggcaacggcg atgcgtttgc
      121 gaaccctttg tcggacactg actggctgcg tcttgcgatg gtgaaggatc tgcaacctgg
      181 cgaaatgacc gctgatgcag aagatgacac ttatctcgat gatgaagatg cagactggaa
      241 aacgacaacc caggggcaga aatccgttgg tgatacttcg gcgacgctgg cctggcgtcc
      301 gggtgacagc gggcagaaaa aactggttca gttgttcgac tccggtgaag tctgcgcgtt
      361 tcgtatcaaa tatcccaacg gcactgttga tgttttccgc ggctggctga gctcactggg
      421 taaaaccatt gcctcaaaag acgtgatgac ccgcactgtg aaaatcagcg gtgtggggcg
      481 tccgtatctg gcagaggaag gcactgaaac cgtgggtgtt accggactga cggtggcacc
      541 ggcatctgtc agtgtcaaag cgggagcaac caccacactg acctttacag taaaacctga
      601 cggggccagt gacaaagcga tcagtgtgca ttcgtcagat cctcagactg cttcggtgac
      661 cctgagcggg cttgtggcca cggtgaaagg cgtgaagcag ggcagtgtca gcatcgtggg
      721 catgacctct gacggagagt ttgtggcagt ggctgcggtg accgtcagcg caccataaca
      781 ggacgatact catcattgcc ccggttatcc ggggcttttt gcatccggag aacatgatgt
      841 ttctgaaaca ggatacgttt aattatgaaa aacagtccgt ggtgctcagt gagctgtccg
      901 ggctgcagag aattgaatat ctgacgtttg ttcagcagcg aacggcaaag tttgatgcac
      961 aggagggaga actgccggag gctgaacgac agattgcttt tctgcgtatg ggaatggata
     1021 tcaatgcctg gctggtttcc cgctcactgt ggaatgctga gcagtctcag gatgttgaga
     1081 cgctttgcgc atccattatg acaacatggt cgtatgatgc gctgggcgcg ggggcggaga
     1141 gggttctgtc gctgagcggt atggggacca ttgagaatgc cggggatgat gatcatgagg
     1201 cgctgacgcc ggaaaagtcc tgacgcggga aatgcagttt gtcatgcggc ttgcccggga
     1261 gttccggcgg gcagactggc ggcggatgct gtcggatatg tcggccactg agcttggtga
     1321 gtggggcgat tatttccgga tgcagagctt cagtgatgtg tggatggatg cgcagtttgc
     1381 ctcgctgaag gcattgatcg tgagaatggt gtccggcagc agcgatgctg cggtggctga
     1441 tttcagcctt ttaccggaag agaacgggat accggagcga acggacgaag aactgatgca
     1501 tcttggggaa ggtatttccg gaggtgtgcg ttatggacca gatagccaac ctggtcattg
     1561 atttggggat tgatgcggca gagtttaaaa atgaaattcc ccgtatcaaa aaccttctga
     1621 atggtgcagc cagcgatgca gaacggtctt ctgcccgtat gcagcgtttt atggagcgtc
     1681 agactcaggc ggcccggcag acaatgcagg cggcttcttc ggctgcaaca gcagcatcag
     1741 cccatgcgca gacggtggag aagaatgcac gggctcatga acgcatggcc cgtgaggtgg
     1801 aacaaacccg tctgcgcgtg gatgccctga atcagaaaat gcgcgaggaa caggcgcagg
     1861 ccagggcact ggcggaggcg caggataaag cggctgccgc cttttatcgc cagattgaca
     1921 gtgtgaaaca ggccggtgcg gggcttcagg aattacagcg tattcagcag cagatccgac
     1981 aggccagaaa cagtggcggg gttggtcagc aggattatct ggcgctgatt tcggagatca
     2041 cggcgaaaac ccgtgccctg acgcaggcag aggaacaggc cacccggcag aaagcggcgt
     2101 ttatccgcca gcttaaagag caggcaaccc gccagaatct gtcgtcttct gagttgcttc
     2161 gcgccagggc ggctcagctg ggggtaagca gtgctgcaga agtgtatatc cgcaaaatgg
     2221 agcgggcagg aaaagccaca cattcgctgg ggctgaaaag tgcggcagcc cggcgggagc
     2281 tgggggtgtt aatcagtcag atggcgcgag gcaattttgg tgcgctgagg ggatccggga
     2341 taacgctggc taaccgtgcc ggatggacag gcgcactgat gtcgccgaaa ggcatgatga
     2401 ctggcggcgt tattggcgga cttgtcgcgg cggtcctggg tctgggtaaa gcctggcatg
     2461 acggccggaa ggagggcgag aaatttaacc gtcagctggc gctgacggga cattatgccg
     2521 gtgtcactgt cgggcagttg tggaaactca gccgggccat atccgggaat ggtatcacgc
     2581 aacatgcggc agccggtgcg ctggcacagg tggtgggcag tggggcattt catggaaacg
     2641 atattggtat ggtggcgaaa gctgccgcac agatggagcg atcggttggc cagtcggtca
     2701 gcgataccat aaatcagttt aagcggctga aggatgatcc tgtaaatgcc gcgaaggcgc
     2761 tggacaatgc gctgcatttt ttgaatgcca ctcagcttga gcagatacgc gtccttgggg
     2821 agcaggggcg gtccagtgat gcggcccgga tcgccatgtc tgcgctggca gaggaaaccg
     2881 gtaaacgcac atctgatatt gataataatc tcaatgcgct gggtagtacg ctgcaaacct
     2941 tgtctgactg gtggaagcag ttctgggatg cggccatgaa tattggtcgt gaagactcgc
     3001 tggatgcgca gattgatgcg ttacaggaaa aaattcagcg cgcgaaaaaa tatccgtgga
     3061 caaacgcctc cacacaggtg gagtacgatc agcagcgtct taacgatctt caggagaaaa
     3121 aacgccggaa ggatttgcag gatgcaaaag cgcaggcaga acggaattac caggagcaac
     3181 agaaacgccg gaatgctgaa aatgccgcgc tgaaccggat gaatgaaacg gaagctgcac
     3241 gacatcagcg ggaaattgcg cgtattaatg ccatgcagta cgccgaccag gctgtcaggg
     3301 atgcggcgat acagcgtgaa aatgaacgtt acgaaaaagc cattaagaaa aatacacggg
     3361 caacccgtaa tgatgaggcc acccggttat tgctgcagta cagtcagcag caggcacagg
     3421 tggaagggca gattgctgct gccagacagt cagcaggcat tgccactgaa aggatgactg
     3481 aagcgcataa acagcttctg gccctgcagc agcgcatcag cgacctggac gggaaaaagc
     3541 tgacggcaga tgaaaagagt gtgctggccc gtaagaatga gctgattcag gcgctgacgc
     3601 tgctggatgt gaaacagcag gagctgcaga aacagacagc gcttaacgac ctgagaaaaa
     3661 aaacggttca gctgaccagc cagctggcag acaaagaacg tgcactgcgt gagcagcaca
     3721 atctggatat tgccactgca ggtatggggg ataagcagcg gcagcgctac caggcacagt
     3781 tgcgcatccg gcaggaatac cggcaacagt tgcaacagct tgagaatgac agtcgccaga
     3841 aaggcactta cgggacggag gactaccgga gggctgagga ggtgctgaag gggagcctga
     3901 agcgacaact gaatgaaaac aaacgctact ggcaggaact ggaagtggcg cagggcgact
     3961 ggaaaaacgg tgccatgcgg gcgtttcaga attttacggc ggatgcggat aatgcggcgg
     4021 gaacggcaga acagatgttt acagtggcat tcagcagtgc cggtaatgca ctggcgacat
     4081 tctgtaccac cggtaagctg aattttaaat ccttcacctc ttccctgttg tcagatatgg
     4141 ccagaattat ggcacagatg gccatgatgc aggcggtaaa gggcgtcggt tctttattcg
     4201 gcttcacgac taatgctgat ggcggtgttt accagtctgc tgatttgagt cgctacagtg
     4261 gcacggtggt taaccgtccg acgttttttg cttttgcaaa aggcgcgggt gtgatggggg
     4321 aagcgggacc tgaagccatt ctgccactgc gtcgcggtgc tgatggtaag ctgggggttg
     4381 tggcggatac tggtggttca ggcatggtga tgtttgcccc gcagtacaac attgagatca
     4441 ataacgacgg cacgaacggg cagataggtc cggctgccct gaaggtggtt tatgacctcg
     4501 ggaaaaaagc ggccgcggac tttatgcaac agcaggcccg tgatggtggc cggttaagtg
     4561 gagcataccg gtaatggaga cgtttcactg gaaagtgcgc ccggatatga atgtggtatc
     4621 agagccgaaa gtggtgacag tgaagctggg cgatggttat gaacagcgtc gtgcggcggg
     4681 actgaataac cagttgtcga cttacagcgt gacgatacgt gttcgtaaag gtgaacaccc
     4741 atctttaaaa gcctttctgg aacggcacgg tggcgtccgc gcatttcagt ggacgccacc
     4801 ttatgactgg aaactgatca gggtggtttg tcgtaaatgg tcggcaagcg tgggggcgtt
     4861 atgggtgact gtaacggccg attttgaaca ggtggtgaac tgatgcagga tatccggcag
     4921 gaaacactga atgaatgcac ccgtgcggag cagtcagccc gggtggagct ctgggaaatc
     4981 gatctgacag aggtcggtgg tgagcgttat tttttctgta atgagcagaa cgaaaaaggt
     5041 gagccggtca cctggcaggg gcggcagtat caggcatacc ccattcaggg gtcggggttt
     5101 gaactgaacg gcaggggctg tgctgcccgt ccgacactga cggtctctaa cctgcacggc
     5161 atggtcaccg ggatggcgga agatctgcag agtctggtcg gcggaacggt ggtccggcgt
     5221 aaggtttacg cccgttttct ggatgcggtg aacttcgtca acggaaacag cgacgccgat
     5281 ccggaacagg aggtgatcag ccgctggcgc atcgagcagt gcagcgaact gagcgcggtc
     5341 agtgcctctt ttgtgctggc cacaccaacg gagacggatg gcgcggtttt cccggggcgt
     5401 atcatgctgg cgaatacctg tatgtggact taccgttctg atgagtgtgg ttacacgggc
     5461 agggcagtgg ctgacgagtt cgacaaacca acaacggata tccggaagga caaatgcagc
     5521 aagtgtatgc gcgggtgtga gttgcgcaac aataccggta atttcggcgg tttcctttcc
     5581 atcaataaac tttctcagta aatccatgac acagacagaa tcagcgattc tggcgcacgc
     5641 ccggcgatgt gcgccagcgg agtcgtgtgg cttcgtggtg agaacgccgg agggagacag
     5701 gtatcttccc agcgagaata tctccggtga gccggaggaa cggttccgga tggctccgga
     5761 ggactggctg cgggcacaaa tgcagggtga gattgtggca ctggtccaca gtcatcccgg
     5821 tggtctgccc tggctgagtg aggctgaccg gcggctgcag gtgcagagtg atttgccgtg
     5881 gtggctggtc tgccggggga cgattcacaa gttccgctgt gtgccgcatc tcaccgggcg
     5941 gcgctttgag cacggggtga cggactgtta cacgctgttc cgggatgctt atcatctggc
     6001 ggggattgag atgccggatt ttcatcgcgg ggatgactgg tggcgtcacg gtcagaatct
     6061 ctatctggat aatctggagg ccacagggct gtatcaggtg ccgttgtcat cagcacaacc
     6121 gggcgatgtg ctgctgtgct gttttggttc atcggtgccg aatcatgccg ccatttactg
     6181 cggcgatggt gagctgctgc accatattcc tgaacaactg agtaaacgag agaggtatac
     6241 cgacaaatgg cagcgacgca cacactccct ctggcgtcac cgggaatggc acgcatctgc
     6301 ctttacgggg atttgcaacg atttggccgc cgcatcgacc ttcgtgtgaa aacgggggct
     6361 gaagccatcc gcgcactggc cacacagctc ccggcgtttc gtcagaaact gagtgacggc
     6421 tggtatcagg tgcgcattgc cgggcgtgat gcaggtgaaa ccgaattgtc tgcccgtctt
     6481 aatgagccgc tggcaaatgg tgcagtgatc cacatagtac cgcgtctggt gggagctaaa
     6541 agtggcggtg tgtttcaggc ggtgctgggg gcagctgtta tggcggttgc tatatggatg
     6601 ccgggggtag gaattatggc gagtaatctg ctgttttctc tcggtgccag tatgacgctt
     6661 ggcggtgttg cacagatgct ggcaccgaaa gccagaactc cccgtacaca gacaacggat
     6721 aacggcaaac agaacacctg tttctcctca ctggataaca tggttgccca gggcaatgtt
     6781 ctgcctgttc tgtacggtga aatgcgcgtg gggtcgcggg tggtatctca ggagatcagc
     6841 acggcagacg aaggggatgg tggtcaggtt gtggtgattg gtcgctgatg caaaatgttt
     6901 catgtgaaac cgcctgcggg cggttttgtc gtttatggag cgtgaggaat gggtaaaggc
     6961 agcagtaagg ggcatacccc gcgcgaagcg aaggacaacc tgaaatccac gcagttgctg
     7021 agtgtgattg atgccatcag cgaagggccg gttgacggtc cggtggatgg attaaaaagc
     7081 gtgctgctga acggtacgcc ggtcctggat agcgagggga agacaaactt ttccggtgtt
     7141 acggtggtgt tccgcgccgg cgagcaggag cagacaccgc cggaggggtt tgaatcttcc
     7201 ggctcagaga ctgtgctggg tacggaagtg aaatacgaca cgccgatcac ccggaccatc
     7261 acgtcggcaa acattgatcg tctgcgcctg accttcggtg tgcaggcact ggtggaaacc
     7321 acctcaaagg gggaccggaa tccgtcggaa gtccgcctgc tggttcagat acagcgtaac
     7381 ggtggctggg tgacggaaaa agacatcacc attaagggca aaaccacttc gcagtatctg
     7441 gcctcggtgg tggtgggtaa cctgccgccg cgcccgttta atatccggat gcgcaggatg
     7501 acaccggaca gcaccacaga ccagctgcag aacaaaacgc tctggtcgtc gtacaccgaa
     7561 atcatcgatg tgaaacagtg ctacccgaac acggcgctgg tcggcgtgca ggtggattca
     7621 gagcagttcg gtaaccagca ggtgagtcgc aattatcatc ttcgcgggcg cattctgcag
     7681 gtgccgtcga actataaccc gcagacgcgg caatacagcg gtatctggga cggaacgctt
     7741 aagccagcat acagcaacaa catggcctgg tgtctgtggg acatgctcac tcatccgcgc
     7801 tacggcatgg ggaaacgtct tggtgcggca gatgtggaca aatgggcgct gtatgtcatc
     7861 ggccagtact gcgaccagtc agtgccggac ggatttggcg gcacggagcc gcgcatcacc
     7921 tgtaacgctt acctgaccac acagcgtaag gcgtgggatg ttctcagtga tttctgctcg
     7981 gcgatgcgct gtatgccggt atggaacggg cagacgctga cgttcgtgca ggaccgaccg
     8041 tcggataaag tgtggaccta taaccgcagt aatgtggtga tgccggatga tggcgcgccg
     8101 ttccgctaca gcttcagcgc cctgaaggac cgccataatg tcgttgaggt gaactggatt
     8161 gatccggaca acggtcatga gacggcgaca gagcttgtgg aggacacgca ggccattgtc
     8221 cgttacggtc gtaacgtcac gaagatggat gcctttggct gtaccagccg gggacaggcg
     8281 caccgcgccg ggctgtggct gattaaaacg gaactgctgg aaacgcagac cgtggacttc
     8341 agcgtcggcg cagaagggct tcgccatgta ccgggtgatg tcattgaaat ctgcgatgat
     8401 gattatgccg gtatcagcac cggtggtcgc gtactggcgg tgaacagcca gacccggacg
     8461 ctgacgctcg accgtgaaat cacgctgccg tcctccggta ccacgctgat aagcctggtt
     8521 gacggaaatg gcaatccggt cagcgtggag gttcagtccg tcaccgacgg cgtgaaggtg
     8581 aaagtgagcc gggttcctga cggcgttgca gaatacagcg tgtgggggct gaagctgccg
     8641 acgctgcgcc agcgcctgtt ccgctgcgtg agtatccgtg agaacgacga cggcacgtat
     8701 gccatcaccg ccgtgcagca tgtgccggaa aaagaggcca tcgtggataa cggggcacac
     8761 tttgacggtg accggcgcgg cacggtgaat ggtgtcacgc cgccagcggt gcagcatctg
     8821 accgccgaag tcaccgcaga cagcggggaa tatcaggtgc tggcgcgatg ggacacgccg
     8881 aaggtggtga agggtgtgag cttcctgctt cgcctgaccg tggcagcgga cgacggcagt
     8941 gagcggctgg tcagcacagc caggacgacg gaaaccacat accgcttcag gcagctggcg
     9001 ctggggaatt acagtctgac agtccgggcg gtaaatgccc gggggcagca gggcgatccg
     9061 gcgtcggtat cgttccggat tgcggcaccg gcagcgcctg tcactattga actgataccg
     9121 gggtattttc agataacggt ggtcccgaaa ctggctgtat atgacccgac ggtgcagttt
     9181 gagttctggt tttcggaaaa gcggattgcg gatatcaggc aggttgaaac cagcgcccgc
     9241 tatcttggca cggcgctgta ctggatagcc gccagtatca atatcaggcc gggccatgat
     9301 tattattttt acgttcgcag tgtgaacacc gttggcaaat cggcatttgt ggaggccgtc
     9361 ggtcgggcga gcgatgatgc ggaaggttat ctgagttttt ataaagggtt gatcaataaa
     9421 acgcatctcg gcaaggagtt gtggacgcag attgataacg gtcagcttgc gccggacctg
     9481 actgaaatca ggacgtccat tacgaatgtc agcaatgaaa tcacgcaaac cgtcaataaa
     9541 aaactggaaa atcagagtgc tgcaatccag cagatacaga aagttcaggt tgatacaaat
     9601 aataacctga acagcatgtg ggctgtgaag ctgcaacaga tgaaggacgg acgcctttat
     9661 attgcgggta tcggtgccgg tattgagaat acgccagcag gaatgcagag tcaggtgctg
     9721 ctggcggcag acaggattgc gatgattaat cctgcgaatg gcaacacaaa gccgatgttt
     9781 gttggtcagg gcgatcagat attcatgaac gacgtgttcc tgaaacgcct gacggctccg
     9841 accattacca gcggcggtaa tcctccggca ttttccctga cacctggcgg acggctgacg
     9901 gcgaaaaatg ccgatatcag cggtaacgtg aacgcgaact ccgggacgct caacaacgtc
     9961 acgattaata agaactgtcg ggttctggga aaattgtccg cgaaccagat tgaaggcgat
    10021 ctcgttaaaa cagtgggcaa acctttccca cgggactccc gggcaccgga gaggtggcca
    10081 tcagggacca ttaccgtcag ggtttatgac gatcagccgt ttgatcggca aattgttatt
    10141 cccgcggtgg cgtttcgcgg tgctaaacat gagcggaaga ataacaatat ttattcgtca
    10201 tgccgcctga tagtgaagaa aaacggtgct gaaatttata accgaacgac cctggataat
    10261 acgctgatat atacgggtgt tattgatatg cctgccggtc acggtcacat gacgctggag
    10321 ttttctgtat cggcatggct ggtaaatggc tggtatccca cagcaagtat cagcgatttg
    10381 ctggttgttg tgatgaagaa atccacagca ggtatcagta tcagctgaat tttataaccc
    10441 agatacgggc accagaaatg gtgccttttt tattgcagaa aagcgagagg taattatgcg
    10501 taaactttat gctgccattt tgtccgcagc catctgtctg gctgtatccg gtgcgcctgc
    10561 atgggcgtct gaacatcagt ccacgctgag cgcggggtat cttcatgcct cgacgaacgc
    10621 tcccggcagt gatgatctga acgggattaa cgtgaaatac cgttatgagt ttacggacac
    10681 gctggggctg gtgacgtcat tcagctatgc caacgctgaa gatgagcaaa aaacgcatta
    10741 cagtgatacc cgctggcatg aggattccgt gcgtaaccgc tggttcagcg tgatggcggg
    10801 gctgtctgtg cgcgtgaatg aatggttcag cgcgtatgcg atggcgggcg tggcttacag
    10861 ccgtgtgtcg accttctccg gggattatct ccgcgtaact gacaacaagg ggaaaacgca
    10921 cgatgtgctg accgggagtg atgacaaccg ccacagcaac acgtctctgg cgtggggggc
    10981 tggcgtgcag tttaacccga ccgaatccgt ggccattgac cttgcttatg aaggttccgg
    11041 cagtggcgac tggcgcactg acggtttcat cgtgggtgtc ggttataagt tctgattagc
    11101 caggtaacac agtgttatga cagcccgctg gttcaggcgg gctttttgtg gggtgaatat
    11161 ggcagtaaag atttcaggtg tactgaaaga tggggcgggt aaacctgtcg taaattgtgc
    11221 gattgaactg cgggccagaa gaaccagtcc gaccgttgtg gcacacgttg ttgccacttg
    11281 cgtgacggac aataacggtg cttatgtgat tgaggctgag ccggggtatt acgaggttgc
    11341 gcttcactgt aacggctggc agccaacccg tgtcggggat attgatgtgg caccgactga
    11401 tgcaccgggg acactgaacg cgtttctgaa tgcaccaaag gatggtgatt tacgtccgga
    11461 ggtgatgaag cgctttgagg aaatggtggc gcaggcgcag cagagtgccg gggctgcagc
    11521 cggaaacgca cagcagacgg cgcaggatgt ggcggcagcc gcaacggccc gtgatgatgc
    11581 acaacgtttt gcggagaaag cccgacagga tgcaaccgtc acagctgagg acagaaaggc
    11641 cactgcggaa gatgtgacaa gcacaggagc aaatgcagcc gcagccggac agagcgcaca
    11701 ggatgccgca ggttatgccc gcgcagcaga acaggccaaa aatgacattg atgctgcgct
    11761 gaccggcact ctgaaaatgg ctaaccatct gtc
//
LOCUS       DAZSIO010000124        11725 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZSIO010000124 DAZSIO010000000
VERSION     DAZSIO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11725
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.124"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..2264
                     /locus_tag="ACHX5T_003025"
                     /pseudo
     CDS             1..2264
                     /locus_tag="ACHX5T_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            2265..3914
                     /locus_tag="ACHX5T_003026"
     CDS             2265..3914
                     /locus_tag="ACHX5T_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR8468117.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3919..4695
                     /locus_tag="ACHX5T_003027"
     CDS             3919..4695
                     /locus_tag="ACHX5T_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR8468118.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            4970..7819
                     /gene="rcsC"
                     /locus_tag="ACHX5T_003028"
     CDS             4970..7819
                     /gene="rcsC"
                     /locus_tag="ACHX5T_003028"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR8468119.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(8019..8669)
                     /gene="rcsB"
                     /locus_tag="ACHX5T_003029"
     CDS             complement(8019..8669)
                     /gene="rcsB"
                     /locus_tag="ACHX5T_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR8468120.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(8686..11358)
                     /gene="rcsD"
                     /locus_tag="ACHX5T_003030"
     CDS             complement(8686..11358)
                     /gene="rcsD"
                     /locus_tag="ACHX5T_003030"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR8468121.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         2841 a         2837 c         3156 g         2891 t
ORIGIN      
        1 attaacctcg tcgctgaaag gtaaacctgt ttctgcgcag ctaacggtag gcgtggtcga
       61 tgaaatgatc tacgcgctgc aaccagaaat cgcgccgaat atcggcaaat ttttctatcc
      121 gctggggcgt aacaatgtgc gtaccagctc cagtctgtcg tttatcagct atgaccaggc
      181 gctctccagc gagccggttg cgcctggcgc gactaaccgc agcgagcggc gagtaaaaat
      241 gcttgaacgt ccacggcgtg aagaggtgga taccgcggca tggatgccgt cactcacaac
      301 cgataaacaa ggcaaagcgt atttcacgtt cctgatgcct gattcgttaa cccgctggcg
      361 tatcaccgcg cgtgggatga acggcgacgg gctggtcggg caggggcgtg cttatctgcg
      421 ttcggaaaaa aatctctaca tgaagtggag tatgccaacg gtgtatcgcg tgggcgacaa
      481 accggcggca ggactgttta tcttcagtca gcaggataac gaaccggtgg cgctggtgac
      541 taaatttgca ggcgctgaga tgcgccagac gctgacgctg cacaaagggg cgaattatat
      601 ttcgctgacg cagaatattc agcaatctgg cttgttaagt gcagaactgc aacaaaatgg
      661 gcaagtgcag gacagcatta gcacaaaact gtcttttgtg gacaacagct ggcccgttga
      721 acagcagaaa aatgtcatgc tcggcggtgg cgagaacgcg ctgatgttgc ccgagcaggc
      781 gagtaatatc cggctacaaa gtagtgaaac gccgcaggag attttccgca acaatcttga
      841 tgcgttagtc gatgaaccgt ggggtggggt gatcaacacc ggtagccgtc tgatcccgct
      901 cagtctcgcc tggcgttcgc ttgccgatca tcaaagtgcc gccgctaacg acattcgtca
      961 gatgattcag gataaccgtc tgcggctgat gcaactggcg gggcccggag cgcgctttac
     1021 ctggtggggt gaagatggca atggtgacgc cttccttacg gcatgggcat ggtacgccga
     1081 ctggcaggcc agccaggcgc tcggcgtaac gcaacaaccg gaatactggc agcatatgct
     1141 cgacagttat gccgagcagg cggataatat gccgttattg catcgggcgc tggtgctggc
     1201 gtgggcacag gagatgaatc tgccgtgcaa aacgttgttg aaaggggggg atgaagctat
     1261 cgcccggcgc ggaactaaag atgaagattt ctctgaggaa gacatccgcg atatcaatga
     1321 cagcctgatc ctcgatacac cggaatctcc actggcagat gcggtggcaa acgtcttaac
     1381 catgacgttg ctgaaaaaag cgcagttgaa gtccacggtg atgccacagg ttcagcaata
     1441 tgcgtgggat aaagcggcaa acagcaatca gccgctggcg cacacggttg tgctgctcaa
     1501 tagcggtggt gacgctaccc agacggccgc tattttaatg gtttgaccgc tgagcaatcc
     1561 actattgagc gcgcgctggc catgaactgg ctggcgaaat atatggcgac aatgcctcca
     1621 gttgttttgc ctgcgcctgc gggcgcatgg gctaaacata agttaactgg agggggcgaa
     1681 gactggcgtt gggttggtta gggtgtgccg gacattctct cttttggtga cgaattatcg
     1741 ccgcaaatgt gcaggtccgc tggcgtgaac cggcaaaaac ggctcaacaa agtaacattc
     1801 cggtgaccgt tgaacgccag ttgtatcggc ttatccccgg tgaagaagag atgagcttta
     1861 ctctgcagcc ggtgaccagc aatgagattg acagcgatgc gctgtatctc gatgaaatta
     1921 cgcttaccag cgagcaggat gcagttctgc gctatggtca ggtggaagta ccgctcccgc
     1981 cgggagctga cgttgagcgc acaacatggg gcatttcagt caataaaccc aacgccgcga
     2041 aacagcaggg gcaattgctg gaaaaagcgc gtaatgaaat gggcgaactg gcctatatgg
     2101 tgccggtgaa agaactgacg ggaacggtca ctttccgcca tttgctgcgc ttctcgcaaa
     2161 aagggcaatt cgttctgcct cctgctcgtt atgtgcgttc ctatgcacct gcgcagcaaa
     2221 gtgtcgcggc agggagtgaa tggaccggga tgcaggtgaa ataagtgaac tggcgcagaa
     2281 tcgtctggct attggcgttg gttactctgc caacgcttgc ggaggagacg ccgttacaac
     2341 tggtgctgcg cggtgcgcag cacgatcaac tgtatcagct gtcgtcatca ggcgtgacca
     2401 aagtctcagc attacctgac tcgctcacca cgccgttagg gagtttgtgg aaactgtatg
     2461 tttacgcctg gctggaggat acccatcaac cggagcagcc ctaccagtgt cgcggaaact
     2521 ccccggaaga ggtttattgt tgtcaggctg gggagagcat aacgcgcgat accgcgctgg
     2581 tacgttcctg tgggctctat tttgcgccgc agcggttgca tattggcgca gatgtgtggg
     2641 ggcaatactg gcaacagcgt caggcacctg cgtggttagc gtctcttaca acgctgaagc
     2701 cagaaacctc ggtaacggtt aaatcgctgc ttgattcatt agcgacgtta cctgcgcaaa
     2761 acaaagctca ggaagtctta ctggatgtgg tgctggacga ggcgaaaatc ggtgtcgcat
     2821 ccatgctcgg tagtcgggta cgggtgaaaa cctggagctg gtttgccgat gataaacagg
     2881 aaatccgtca gggtggtttt gctggctggt taaccgatgg tacaccgctg tgggttactg
     2941 gcagtggcac cagcaaaacg gtactaaccc gttatgcgac ggtgcttaat cgggttttgc
     3001 cagtgccgac gcaggttgcc agtgggcaat gtgtagaggt ggagctgttt gcccgctatc
     3061 cgctgaagaa aatcacagcg gaaaagagta cgacggcagt aaaacccagc gtacttaatg
     3121 ggcgctatcg tgtgacgttt actaatggta accacattac gtttgtcagc cacggtgaga
     3181 cgaccttact gagcgaaaag ggcaaactga aattgcagtc gcatctcgat cgtgaagagt
     3241 acgttgccag agttctggat cgcgaggcga aaagtacgcc acctgaggca gcgaaagcca
     3301 tgactgtggc gatacgtact tttttacagc aaaacgccaa tcgggaaggt gattgcctga
     3361 ccatccccga cagcagcgcc acgcagcgtg tttctgcttc gccagcgacc acgggggcgc
     3421 gaacgatgac cgcctggacg caggagctca tctatgcagg cgatccggtt cattatcatg
     3481 gcagtcgcgc taccgaaggg acactttcct ggcggcaagc cacggcccag gcggggcagg
     3541 gggagcgtta cgaccagata ctcgcgtttg cttatcccga caacagcctt agccgctggg
     3601 gagctccacg ctcaacctgc cagttattac ccaaagcgaa agcctggctg gcgaaaaaaa
     3661 tgccgcagtg gcggcgtata ttacaaggcg agacggggta caacgaacca gacgtgtttg
     3721 cggtctgtcg tttagtctca ggtttcccct ataccgatcg gcagcagaaa cggctgttta
     3781 ttcgtaactt ctttacgctt caggatcggc tggatttaac ccatgagtat ctgcacttgg
     3841 cctttgatgg ttatcccacc gggcttgatg agaactatat cgaaacgctg acccgacaat
     3901 tattaatgga ctaatgctat gcgaaagatt tttcttccgt tgttactggt ggcactttcg
     3961 cctgtcgctc acagtgaagg tgtgcaggaa gtcgagattg acgcaccgct ctctggctgg
     4021 catccggcag agggcgaaga tgccagtttt tcacaatcga tcaattaccc ggcgtcgtcc
     4081 gtcaacatgg cagatgatca aaatatctca gcgcagatcc gcggcaaaat aaagaattat
     4141 gctgcggcgg gtaaagttca gcagggccgg ctggtggtca acggtgccag tatgccgcag
     4201 agaattgaat ccgatggttc atttgcacgc ccttatattt tcactgaagg cagcaacagc
     4261 gtgcaggtca tcagcccgga tgggcaaagc cggcaaaaaa tgcagtttta ctcaacgccg
     4321 ggtacaggaa ctattcgtgc acgtttacgg ctggttctct cgtgggatac ggacaatacc
     4381 gatctcgatc tccatgttgt cacgcccgat ggcgaacacg cctggtacgg taacaccgtg
     4441 ctgaaaaaca gtggtgcact ggatatggat gtcacgacgg ggtacggacc cgagattttc
     4501 gccatgccag caccagttca cgggcgctat caggtgtata tcaactacta tggtgggcgc
     4561 agcgaaacgg aattgacgac cgcccaactg acgctgatca ccgatgaagg atcggtcaat
     4621 gagaaacagg aaacatttat tgtaccgatg cgtaatgctg gcgagctgac gctggtgaaa
     4681 agttttgact ggtgatccat cccttcacac aacctggctc tctctttttt cagataagac
     4741 tgctgggcga tctgccgacg ccaggatata accattaaca agcgtcggca gattggtcat
     4801 ttcttatttt tcgtactgag aactgtccat ctactccggc tttcctggtt aaggcaatgg
     4861 tttctaagcg atacgcatta ttgcaatggt gacagaacat tgcggtaaca cgcttttacc
     4921 gctaccttaa ccacactcca tcggtcacct gaggcggagc ttctcccctt tgaaatacct
     4981 tgcttctttt cgtacaaccc tgaaagcctc gcgctacatg ttcagagcat tggcgttagt
     5041 gctctggctg ttgattgctt tttcatccgt tttttacatc gttaatgcgt tacatcagcg
     5101 agaatcggaa attcgtcagg aatttaatct gagttccgat caggctcagc gctttattca
     5161 acgcacctct gatgtgatga aagagcttaa gtacatcgcc gaaaatcgct tatcggcaga
     5221 aaacggtgtg ctttccccgc gtggacgaga aacgcaggcg gatgtgcctg cgtttgaacc
     5281 gctgtttgcc gactccgatt gttccgcaat gagtaacacc tggcgaggtt ctctggagtc
     5341 attggcgtgg tttatgcgct actggcgcga taatttttct gcggcttacg atctcaaccg
     5401 ggtattttta atcggcagtg ataacctctg catggccaat ttcggtctgc gtgatatgcc
     5461 agtggaacgc gataccgcgt tgaaagcgtt gcatgaacgc atcaataaat atcgaaatgc
     5521 accacaagat gatagcggca gtaacctcta ctggatcagc gaaggtccgc gcccttgcgt
     5581 cgggtatttt tacgcgttga cgccagttta tctggcgaac cggttgcagg cgcttttggg
     5641 tgtcgagcag accatccgga tggagaactt tttcttaccg ggtacgttgc cgatgggggt
     5701 taccattctt gatgaaaatg gtcataccct gatttcgctt accggaccag aaagtaaaat
     5761 taagggcgat cctcgctgga tgcaggaacg ctcctggttt ggctatacgg aagggttccg
     5821 ggagctggtg ctgaagaaaa atctgccacc ctcatcgcta agcatcgtgt attcggtgcc
     5881 ggttgataag gtgctggaac gcattcgcat gttgatcctt aacgcaattt tgctgaatgt
     5941 gcttgccgga gctgcattgt ttactctcgc gcggatgtac gagcgacgta ttttcattcc
     6001 ggcggaaagc gacgccctgc gactggaaga acatgagcag ttcaatcgca agattgtcgc
     6061 ctccgcgcca gtgggtatct gcattttgcg taccgctgat ggcgtcaata ttttaagtaa
     6121 cgaactggcg catacctatc tcaatatgct tacgcatgag gaccgccaac gactgacgca
     6181 aattatctgt gggcagcagg tcaattttgt tgatgtcctg accagcaaca ataccaatct
     6241 gcaaatcagc ttcgtccatt cgcgctatcg taatgaaaac gtggccattt gtgtgctggt
     6301 ggatgtttct tcgcgcgtga agatggaaga gtcgttgcag gagatggcac aagcagcgga
     6361 acaggcgagc cagtcaaaat cgatgttcct tgccaccgtc agtcatgagc tgcgaacgcc
     6421 gctgtatggc attatcggta acctggatct gttgcaaacc aaagagttac cgaaaggcgt
     6481 cgatcgtctg gtgacggcaa tgaacaactc ttccagcctg ttgttgaaaa ttatcagcga
     6541 tattctcgat ttctcgaaga ttgaatcgga acagttgaag atcgaaccgc gtgagttttc
     6601 accgcgtgaa gtgatgaacc acatcaccgc caactattta ccgctggtgg tacgcaagca
     6661 gttaggcttg tactgcttta ttgaaccgga tgtgccagtg gccttaaatg gcgacccgat
     6721 gcgtttacag caggtcatct ccaacctgtt gagtaacgcc ataaaattca ccgataccgg
     6781 ctgtatagtt ttgcatgttc gcgctgatgg cgattatctc tctatccgtg ttcgcgatac
     6841 cggcgtgggg attccggcga aagaagtggt gcgcttgttt gatcccttct tccaggttgg
     6901 aacgggcgta cagcgaaact tccaggggac cggtctgggt ctggcgattt gtgaaaaact
     6961 gatcagcatg atggacggcg atatctcggt agattcagaa ccgggaatgg gcagccagtt
     7021 taccgtgcgt attccgttgt acggcgctca gtacccgcag aaaaaaggcg tggaagggtt
     7081 gagtggtaaa cgctgctggc tggcggtccg caatgcgtcg ctctgtcagt tcctggaaac
     7141 cagtttgcag cgcagcggca tcgtcgttac aacatacgaa gggcaggaac cgactcccga
     7201 agatgtgtta atcactgacg aggtagtgag taaaaaatgg cagggcagag cggtagtgac
     7261 cttctgtcgt cgccatattg gtattccgct ggagaaagcg ccaggggagt gggtacacag
     7321 tgtggctgct ccgcatgagc taccggcatt gttggcgcgt atttatttga tcgagatgga
     7381 gagcgacgat cctgctaacg ctctgccgtc gacggacaaa gcggtcagcg ataacgacga
     7441 tatgatgatt ctggtcgtgg atgatcatcc gattaaccga cgtctgctgg cagatcagtt
     7501 gggatcgttg ggctatcaat gtaaaaccgc gaatgatggc gtcgatgcgc ttaatgtact
     7561 tagtaagaat catattgata tcgtgcttag cgacgtcaac atgccaaata tggatggtta
     7621 ccgcttgacg caacgcattc gtcagttggg actgacgttg ccggtaatcg gagtaactgc
     7681 taatgcgttg gctgaagaga agcagcggtg tctggagtcc ggtatggaca gctgcctctc
     7741 gaagccggta acgctggatg tgataaaaca gacgctgacg gtatatgccg agagggtcag
     7801 gaaatcgcgg gattcgtagg acggataagg cgtttacgcc gcatccggca ttcagtgcaa
     7861 atgccagata agacactgac gcgtcttatc tggcctactt taatgtaatc tgttgaaata
     7921 atgggaatcg taggccggat aaggcgttta cgccgcatcc ggcaatcggt gcaaatgcca
     7981 gataagacac taacgcgtct tatctggcct acagatgatt agtctttatc tgccggactt
     8041 aaggtcacag aagagagata attcagcagg gcgatatcgt tctcgacgcc cagcttcatc
     8101 atcgcagatt tcttctggct actgatggtt ttaatactgc ggttcagctt tttagcgatc
     8161 tcggtcacta ggaagccttc cgcaaacagg cgcagaactt cactctcttt tggcgagaga
     8221 cgcttgtcac cgtaaccacc agcactgatt ttttccaaca ggcgagaaac gctttccggg
     8281 gtaaatttct tccctttctg cagcgcggcg agagctttcg gcagatcggt cggtgcacct
     8341 tgtttcagca cgatcccttc gatatccaga tccaataccg cactaagaat cgccgggttg
     8401 ttgttcatag tcagaacaat gatcgacagg cttgggaaat ggcgcttgat gtacttgatt
     8461 aaggtaatgc catcgccgta cttatcgcca ggcatggaga gatcggtaat caacacatgc
     8521 gcatccagtt tcggcaggtt gttgatcagt gctgtagagt cttcaaattc gccgacaaca
     8581 ttcacccact caatttgctc aagtgatttg cgaataccga acaagactat cggatggtca
     8641 tcggcaataa ttacgttcat attgttcatg taataggcta ccttgctaca gcaagctctt
     8701 gacataactg tcaatgtcgc tgatgtattt ttctattcct ggaacatcct tctcacgaat
     8761 cagatgttcc agcgtttcac ataactgctt gccgggtacc agatttagca tggcaaatac
     8821 gcctttaaga cgatgagccg tttgggctaa cgcagcaaag tcactggttg ctgcttcagt
     8881 atacagcctc ttaacatcat ccggtactgt gtctacaaag agcgcataat agccgctggc
     8941 atggagttgc gcattttcat ctccgcccag aggcgattct gtcacctctt cctgtgccag
     9001 ttgcacttca attaattgta agaccgcttc ctgcatagcg ttgctcatat tgaagttgac
     9061 gcacaattga ccaggcccaa tttctcgtac gccagactca tcatcgctta aaagcaagcc
     9121 agaggcagta agattagacg gattatccgt taaaaagata tcataatctt gactaattaa
     9181 tctttcatcg ggtgtgatac aggttgcacc ccaattttct aacaggcgag tgacaatatt
     9241 ccgaatttct gccgaagtaa catccaccat tacgcagaca tcatccagta aacgctcttc
     9301 ttcctcttca acttccgggt cagcggcgag cattttgata tgcacagagt agcgtgtacc
     9361 aagcccatcc cgcgttttga tgtttaaatg accgcccagt ttacgtgcca gttgatcgct
     9421 cagccagaat gccagcgggt ccgcctttcc ataacgatcg ttttgggtct ggttgataaa
     9481 cgggaagtgc aaattatcca tttcatgaat acttacgcct tctcccgtgt ccagaatgcg
     9541 gaatgtcagg cggtcttcag aggactcatc ctgatcaacc tcaagggtga tttttcccaa
     9601 ttgcgttgag gtcacggcat attgcatcag tagcagcaaa atacgtcgta aggcatcgcg
     9661 atcgccgcgg cgcatatcgt gtgctttcag atgattgtta atcagcagtt gcagaccttt
     9721 acgcttgatg gcaggcaaca ctgaaggcac aacttcatca attaaatcct gcacggagaa
     9781 cagcaccgtc tcacttttcc agctatcatc cgcaagcatg ttcgctaact gtatttcttc
     9841 gaccagccgc accagcacat ctgcctgatt cgccagttgt ttgctttccg gggcgttgag
     9901 tttagccgca ctctccgcca gggactgtgc gggttctttc agcgcatcgc caatgttttt
     9961 cataaaggtc atccgcccct gctggttttt ctcatacaga cgctgcgcct gcttgagttt
    10021 cttgtttacc agcacttcgc gatcctgatc gcgaataatg aaaatttgtg tgcgcggcgc
    10081 gacctggctg cggaacatgc ggatctcata cagctcgtta ttgatcgtcg cctgaataat
    10141 cccctgatgc tgttccgcca tggtggtgat gttttgcaga ttcagatgcg gcagcaaatg
    10201 atcggcaatt ttgttactta tgacagtgcg gttcgattcc tgatcgtgaa ccagcaggcc
    10261 gagcggcaac agtgagacta tctcttcatt gattgcccgt aaaatgcgca attcgttatt
    10321 gaccgccgtg ctggggacgc tttctgtact gcggctggag aaatggcgga atgtggtata
    10381 gccaaataac gccagcgcca gcaaaccgat gttcagcagc agtggcagca gaatgttttg
    10441 caacgtatcc agcaataagg tgccataagg aacctgccag accaggcgca tatcggtaga
    10501 gttgagtgcc gaggagattt caatcttcgt actgttaaag tggatactga cgctatccgt
    10561 cccttctttc tcattatcat tgtttcccgt tgccgtcgcg tctggctcaa ggcggaaact
    10621 gtccagcggc atacccggtg ggatcaaatc attaatcggc agatcaaaag ccacgaccgt
    10681 tgccagatgt cccggctggt tgaacgtggt acgcaaggta aagtaatgac cgttctgcca
    10741 ggctaggcgg cgcatgttag aaaaactttc gcgttcatcg agggcgttgg cctgttgtaa
    10801 catttctgca cgacgtgaat caacaatgtc actgacggtc gattctttaa atccggaggt
    10861 gagatcttta aggggtaggg ttgagatcag caccagactg ttatcctgac cattcaggta
    10921 atacatcgac cacggtacat tttctgcacc ccacaatgta tccagataag tggacatccg
    10981 ctgagtcatc tcaagcgttg agttgtcgtg agagccaaag attaacgctt ccgttttgcg
    11041 gcgtggtttc tccagatagt agacatcctg tttcaggcgc gtctcttgta aaccttcgcc
    11101 ggaggagggg gaggtcgtcg cggcaatgtt gtcgtagatc tgcctggtca cgtaacgcca
    11161 ggtatcgacg cgcttttgga ttgcgtgggt aatgtcgaca atctggtaac ttttatcttt
    11221 cagccaagcg ttaacggcac tttgtaccat tacccccatc gtcaccagta acacaatgat
    11281 caacagtaaa aagaagcggg taatgctccc cggtaggagt gaaaagcggg tcgtggccgt
    11341 tgtctctttc tgacgcattc gtgtttatga cctgttaaaa cttcgcgaag tgatagggca
    11401 gtataaaggg tacagtgtga aattccagac tcttacctga ttgatgattc tctattctcc
    11461 cgtgatattt atcatcctgt atggggggaa atagatgtac aagcgccatt ttgctgtaca
    11521 aataacctac aaaaagccca acgaaattaa ttgcaaataa acaaagattg ctggaaatta
    11581 tgctgatgtt aattatttgt gaaatagtta acaagcgtta tagtttttct gtggtagcac
    11641 agaataatga aaagtgtgta aagaagggta aaaaaaccga atgcgaggca tccggttgaa
    11701 ataggggtaa acagacattc agaaa
//
LOCUS       DAZSIO010000125        11682 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZSIO010000125 DAZSIO010000000
VERSION     DAZSIO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.125"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            47..610
                     /locus_tag="ACHX5T_003031"
                     /pseudo
     CDS             47..610
                     /locus_tag="ACHX5T_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
     gene            complement(681..1130)
                     /gene="rplI"
                     /locus_tag="ACHX5T_003032"
     CDS             complement(681..1130)
                     /gene="rplI"
                     /locus_tag="ACHX5T_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR8468122.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1172..1399)
                     /gene="rpsR"
                     /locus_tag="ACHX5T_003033"
     CDS             complement(1172..1399)
                     /gene="rpsR"
                     /locus_tag="ACHX5T_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR8468123.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1404..1718)
                     /gene="priB"
                     /locus_tag="ACHX5T_003034"
     CDS             complement(1404..1718)
                     /gene="priB"
                     /locus_tag="ACHX5T_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR8468124.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1725..2120)
                     /gene="rpsF"
                     /locus_tag="ACHX5T_003035"
     CDS             complement(1725..2120)
                     /gene="rpsF"
                     /locus_tag="ACHX5T_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR8468125.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2446..2721
                     /gene="yjfY"
                     /locus_tag="ACHX5T_003036"
     CDS             2446..2721
                     /gene="yjfY"
                     /locus_tag="ACHX5T_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFR8468126.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(2850..3536)
                     /gene="ulaF"
                     /locus_tag="ACHX5T_003037"
     CDS             complement(2850..3536)
                     /gene="ulaF"
                     /locus_tag="ACHX5T_003037"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR8468127.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(3536..4390)
                     /gene="ulaE"
                     /locus_tag="ACHX5T_003038"
     CDS             complement(3536..4390)
                     /gene="ulaE"
                     /locus_tag="ACHX5T_003038"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR8468128.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            complement(4400..5050)
                     /gene="ulaD"
                     /locus_tag="ACHX5T_003039"
     CDS             complement(4400..5050)
                     /gene="ulaD"
                     /locus_tag="ACHX5T_003039"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFR8468129.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5064..5528)
                     /gene="ulaC"
                     /locus_tag="ACHX5T_003040"
     CDS             complement(5064..5528)
                     /gene="ulaC"
                     /locus_tag="ACHX5T_003040"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR8468130.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(5538..5843)
                     /gene="ulaB"
                     /locus_tag="ACHX5T_003041"
     CDS             complement(5538..5843)
                     /gene="ulaB"
                     /locus_tag="ACHX5T_003041"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR8468131.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(5859..7256)
                     /gene="ulaA"
                     /locus_tag="ACHX5T_003042"
     CDS             complement(5859..7256)
                     /gene="ulaA"
                     /locus_tag="ACHX5T_003042"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFR8468132.1"
                     /translation="MEILYNIFTVFFNQVMTNALLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            7611..8675
                     /gene="ulaG"
                     /locus_tag="ACHX5T_003043"
     CDS             7611..8675
                     /gene="ulaG"
                     /locus_tag="ACHX5T_003043"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR8468133.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            8783..9538
                     /gene="ulaR"
                     /locus_tag="ACHX5T_003044"
     CDS             8783..9538
                     /gene="ulaR"
                     /locus_tag="ACHX5T_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR8468134.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(9535..10284)
                     /gene="yjfP"
                     /locus_tag="ACHX5T_003045"
     CDS             complement(9535..10284)
                     /gene="yjfP"
                     /locus_tag="ACHX5T_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR8468135.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            10466..10795
                     /gene="bsmA"
                     /locus_tag="ACHX5T_003046"
     CDS             10466..10795
                     /gene="bsmA"
                     /locus_tag="ACHX5T_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR8468136.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            10944..11219
                     /gene="yjfN"
                     /locus_tag="ACHX5T_003047"
     CDS             10944..11219
                     /gene="yjfN"
                     /locus_tag="ACHX5T_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR8468137.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(11336..>11682)
                     /locus_tag="ACHX5T_003048"
     CDS             complement(11336..>11682)
                     /locus_tag="ACHX5T_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR8468138.1"
                     /translation="GNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQ
                     QQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQN
                     DLLLRATGGVCV"
BASE COUNT         2959 a         3087 c         2870 g         2766 t
ORIGIN      
        1 ccatcttaaa tgactctgag gtgtctgtta aacccgtggc gattcagtaa caatgaatgg
       61 tgataattct tttggatgga accatgaagc tacattagat aatattcacg cccagaaata
      121 caataaagct tacgcgggtg cgaggttaac cgcagaactg aaatatttat tgcaagatgt
      181 cgaatccttc gaacccaaca gtaaatacac aatacacgag gttgttttgg gacctggtta
      241 tagtactcca gactataccg gccaaacgat aggatacgtt gtaacccttc ctgcccaaat
      301 gcctaattgc tgctcatctg aactaccaac aatcgatctt tatatcgacc aacttcacac
      361 tgttacaggc gtttctaatg ccctcggatt catcattgct gcattgctaa acgcgtattc
      421 tgatttaccc catgatttga aaattggtct acgttcctta tcaaattcag ccgcaatcta
      481 ctcgggttta ggtttcgagc gagtgccgca ggaccgagac ataaggagtg atcgtatgca
      541 tctaacgcca gcaaaccatc ctgacttatg gacacaagag aatggtgaat ggatctactt
      601 gcgaaattaa tgcctgacga aaaatagaaa gcaaaacgcc gaccaatggt cggcgttttt
      661 acgtctcgtt gaataacgaa ttattcagct actacgttta cgatcacttt cgcgaatact
      721 tcgctgtgaa cctggaagct cacttcgtgt tcgccagtgg tacgcagaac gccgttcggc
      781 agacgaactt cgctcttagc cacttcaacg ccagctgcag ttacagcgtc agcgatgtcg
      841 cgagtaccga tggaaccgaa cagtttacct tcgtcgccag ctttagacgc gatggtaaca
      901 gtttccagtg cattgatttt ctcagcgcga gcattagcag ctgccagaac ttcagccagt
      961 ttagcttcca gttcagcgcg acgtgcttcg aagaattcaa tgtttttctt ggtagctgga
     1021 acagctttac cctgcggtac caggaagtta cgagcatagc ccgctttaac gtttacctga
     1081 tcacccaggc tacccaggtt tgctacttta tcaagcagaa taacttgcat taccttatcc
     1141 tctcaaagtc gtattaatgg accgtgaccg attactgatg gcgatcagtg tacggcagca
     1201 gggacaggta gcgagcgcgt ttgatagcgc gagccagctg acgctggtat tttgcacggg
     1261 taccggtgat acggcttggg acaatcttac cgctttcggt gatgtagttt ttcagcgtag
     1321 cgatatcttt atagtcgatc tcttgaacgc cttccgcggt gaaacggcag aacttgcgac
     1381 gacggaaata acgtgccata tggctagtct ccagaatcta tcaattcaat ctgctcggca
     1441 tgcaaaacca ttttgctcag tccgttcttt gccttgtggc atgaaatgaa cccctgaacg
     1501 gttatgcgac tgccgaccgt tatactgtga gtaatggcct ggttttcgtg tccgctaaca
     1561 ataacgggca tttgacacca tgcctgccgg tgaaagccgg cttcctcctg cacagaacga
     1621 tgctcaagca cgaactggca gtgaggaatt cctgatggac tgacctttcg aaggggagtc
     1681 ctgcacacgg tgccggacaa caccagacgg ttggtcatca gaaattactc ttcagaatcc
     1741 ccagcttcag catcatcagc ggtttcgttt gcgaaatcat cgcgacgctc acggcgctcg
     1801 tctttcgctt taaccatcgg agatgcttcg gtaacagcgt gcttggtacg cataaccatg
     1861 ctgcggataa cggcatcgtt gaagcggaag gtagtttcca gctcatcgat cacttcctgc
     1921 ggagcttcaa cgttcatcag aacgtagtgt gctttgtgca gtttgttgat cgggtaagcc
     1981 agctgacggc ggccccagtc ttccagacgg tggatcttgc cttctgcacc agtgatggca
     2041 gcagtgtagc gctcgatcat gcccggaacc tgttcgctct ggtcaggatg gaccataaaa
     2101 acgatttcgt aatgacgcat cgaattgctc cttacggatt attcagcctc ctgtctgggt
     2161 cagccgaatc ccggggaggc aaggaacgtg ttaaaggtcg gctgaaaaat gacgcgttat
     2221 agtactggca taccccgcta aactcaaggg cggagcagaa ataaatctcc cctttcgcaa
     2281 ataacctcta aaccgctgat ttgaagttca tcacattccg tcacagtgtt ttttgaacaa
     2341 ctgcatcaga aatggtcacc atcaacttct ttatcattga ttaaaattaa atcagaagat
     2401 catcacagag gagagcacac aaagtcagcc gtcgaggagg tatctatgtt cagtcgagtt
     2461 ttagcccttc tggctgtgct tttgctaagt gcaaatacat gggcagccat tgaaattaat
     2521 aaccaccagg ccagaaatat ggacgatgtg caaagcttag gcgtgattta tatcaatcat
     2581 aatttcgcca ctgaaagcga agcacgtcag gcattaaatg aagaaacaga tgcgcaaggc
     2641 gcaacgtact accacgtaat tctgatgcgg gaaccgggga gtaacggcaa tatgcacgcc
     2701 agcgcggata tttatcgcta gcaccaggta taaccaacga aacattgcca tagtttgctt
     2761 tgcccccttc gcagggggct ttttttgaaa aacggctgcg gaattagacc agttatctcc
     2821 cgaggaagga aatttccgca gcgcgtgttt tacttctgcc cgtaataagc gttaggaccg
     2881 tgcttacgca tgaagtgttt attcatcagg tagctgtcga tatgattgag ttgtggatta
     2941 atactgcgcg caatccacgc cattttcgcc acttcttcca tcactaccgc gttatgcacc
     3001 gcgtcgtggg catctttccc ccaggcgaac ggtccgtgct gatacaccac aattcccggc
     3061 gtatgcagcg gctcggcgtt gcccagcgtt tcgataatca ctttgccagt gttcagttca
     3121 tactcaccct gcacctcttc ttcgcttaat ccacgcgtac acgggatatc accaaagaag
     3181 tagtcagcgt gcgtggtgcc taatgccggg atcgccagcc ccgcctgcgc ccatgcggtg
     3241 gcatgagtgg agtgggtatg gacaatgccg ccaagcgacg ggtaacgacg gtagagttcg
     3301 agatgcgtcg cagtgtcgga agatgggcga tactcccctt ccaccacctt gccgctcata
     3361 tcgaccacca ccatatcgtc tgctttcatg gtttcgtagg caacgccgct gggcttgatc
     3421 accaccagcc cgcgttcgcg gtcgatagcg ctgacgttgc cccaagtaaa ggtcaccagc
     3481 ccatagcgcg gcagatccat gttggcttca aacacctgct gttttagctt ttgcattatg
     3541 ccgcctccac catgcctgct ttggccatgc gcgctttcac ccaatcacac gctttcacca
     3601 cttctgccgc cgggtcttcc gccgtttcgc tccacatctc aatcaggtac ggcccgcaat
     3661 agccactctg tttgagcgtt tcgaagcaac gttcgaaatc caccacacct tcgccaaatg
     3721 gcacgttttt gaagacgccc ggtttggtgt ctttcacatg caccgcgacg atatgcccga
     3781 ttccggcctg caactccatc tgcacatcgt tgtcccacgc cgacaggttg ccgatatccg
     3841 ggtagagctg gaaccacggg ttgttgagat agtgcgcgta acccagcgcc ttgctgatgg
     3901 agttcatcaa cggataatcc atgatctcca tcgccagcgt cacctgcgcg cggctcgcca
     3961 tctcaacgct ctctttcagg ccgtcacgga aacgacgacg cgtttcgtta ttggcttcct
     4021 gatagtaaac gtcatagccc gccagctgga tcacgcgaat gccaacgtcc tgggcgaact
     4081 ggatggcttt acgcataatc tccagcccct gcgcccgcac cgcgtcatct tcgctgccca
     4141 acgggaaacg acgatgggca gaaaggcaca tggacggcac acgcacgccg gtttcaacaa
     4201 tagcattcac cagcgccaga cgctgttcgc ggctccagtc aaggcgcgac aggcgctcgt
     4261 tagtttcatc taccgacatc tcgacaaaat cgaagcccaa cgtttttgcc agtcgcaggc
     4321 gttccagcca gcactccccg gcggggagcg ctttttcata gatgccaagc gggatttgtt
     4381 tggacaacat atccgctcct tagccccaca gttcggcgat ggaacgtttg aactgacgcg
     4441 cggcttcaac cggagaagcg gcatcacgga tgctgcgacc ggcgataaac acatgaattg
     4501 gaatgccttt gaacagcggc agatcttcca gcgccaggcc accggtgacg gtgactttga
     4561 agcccatatc ggaaagacgt ttgatcgcag tgatatcggc ttcaccccac gccacgccag
     4621 cggcctgcgc gtcacggctg cggtgataaa ccacctgctg aatacctgcg tcgcgccact
     4681 gttgcgcctg ttcccaggtc cagtaaccgg tcagttcgat ctgcacgtcg ccgttaaact
     4741 cttttgctac gtccagcgca cctttggcgg tgttgatatc cgcacaacaa atcacggtca
     4801 cccagtcagc gttggcttcg aagcacatac gagaaagaat tttgcccgca tcggcaattt
     4861 tggcgtctgc cagcacgatt ttgtgcgggt agagcgcttt cagatcgcga accgcacgca
     4921 cgccttcgcc cacgcacaga atggtaccca cttcgataat gtcgacttct tcggcaatca
     4981 ggcgagtggt ttcgtaggcg ctatccatag tctggttgtc cagcgcgact tgcaacatcg
     5041 gtaatgacat cttcaattcc ttcttaagct gccgcgttgg tgcggtcaat gagatccagt
     5101 acttcctgct cggtacggca ggcacgtaaa cggtcgaaat tttcttcatc ttcaaacagg
     5161 ttgacgatct gcatgatgcc cacttcctgg tgcgtgttgg catcgaccgc cgccatggtg
     5221 atgaggatat ccaccgggtc gttatcttcg tggttgaact ccagcggctt tttcagcgtc
     5281 accagcgcaa aaccggtttt cttaacgccc tcttccgggc gaccgtgcgg cattgccagg
     5341 cctggtgcga gcacaaagta cggaccgtgc tgttcaaccg catccagaat cgcctggtag
     5401 taacgcggct cgacaacatc cgccgccacc agcaggtcaa caccgatttt tactgcgtcc
     5461 tgccatgtct cagcttcagc ctgcaggcgg atggatttat tctccgccag cgaatcatgt
     5521 aatttcatgg cgcgtcctta cttcacatcc tgcgggaaat gcgctttgat cacttccagc
     5581 agtttcgggc caaagtcggc aggagagagc atgttgcgca cgccaaccac gtatttgttg
     5641 ccggtcacgg tgatttcgcc cgcaatgtgc gtagaagcga tgatgatatc cgcgccgctc
     5701 aactcgcttt tgtattcgcc aaccgcgcag ctatttaccg tgtggtcaat gtttgattgg
     5761 gttaaaaact ggtccacttt catcttcatg atcatggaac tgccttgtcc gttgccacac
     5821 acagccagaa tacgtacggt cataatcaaa actccttatt aagcagactg ttctgccaat
     5881 tgtttttctg catcttcttc tgcgcgcagt gcgcggccag cgaagaacat ataagccagt
     5941 gcaattacaa tgatgacggc cataaaggcg ataccgatgg agaagaagcc ctgcatcatc
     6001 ggcggtgcca gaatcgacca gtccgccatc cccatccagg cactcatacc ggtaagtttc
     6061 accacccaga cgcaaccaaa gatttcgatc atccccatca ccagacaaat cttcagcgcc
     6121 gcacgccagc cgccgaagtg gttagcgaac acgccgatgg tggcgttgga gaagaacatc
     6181 gggataaagc ctgggataat caggatcgag gaaccgcagg cgaccaggat gccaaccgca
     6241 atcagctgac cgatggtgcc ccacataaag ccccagacca cggcgttcgg agcgaagcta
     6301 taaatagccg cacagtcaat cgccagaacc gcaccaggga tcaggcgctg ggagataccg
     6361 ttaaacgctt cagagagttc cgccacaaac atgcgcacac cctgagtgat gatgaagatc
     6421 gccaccgcga aggaaaaacc ggtttgcagg atatagaccg tccagtgcac tttgcctgcc
     6481 atcgcctgca cggtgtcgat accgaaggag agcagaatgg caccaaagaa gatggtcatg
     6541 acaatcgccg tggagacgat gttgtcgtgg aaaatattca gccagcctgg cagtttgagg
     6601 tcttcaacgc tttcctcttt tttgccgagg aacggcgcaa ctttataggc aattaatgat
     6661 gcaaactgtt gctggtgacc gatggagaaa ccgcagccgt ccgtcacttc ctgagtcggc
     6721 ttgtacatca tgttggaggt aatgccccag tagagcgaaa ccagaatcgc cgtgcagata
     6781 atggttgtcc acatggagta gccgaaaata aacaacgtaa cggcaatcag ccccgcctgc
     6841 tggaacatga tatgaccagt cagcatgatg gtacgaatac cggtaatgcg acgcagcagc
     6901 acgtaacaga tgttcagcgc cagcgctaac aacaccgcgt aacctaccca gctataggca
     6961 tcgcccatgc ggtcgatggt tgccatcatc gacgcgtaag tatcggaaat tgcgccgtta
     7021 atgccgtaga cttcggacat tttcgccacc accggtttga aggtgctggt gaggatgccg
     7081 gaccctgcct gcaacaacat gaaaccaatt atggttttaa tcgtgccttt aataataacg
     7141 ctgacacttt tgcgcagtag gatgtagccc agacaggtca caatacccag caacaacagg
     7201 gcattggtca tgacctggtt aaaaaacacg gtaaagatgt tgtagaggat ctccataacg
     7261 atctcctgaa gaagaggtaa ccgggtattc cgcacacccg gaatgttgtg cactcactct
     7321 aattttcaaa agtaatcaca acaagattat ttatgattaa atgtgacgcg acccgcaaat
     7381 aattccacat ggatagagtg ggattcacac aacgaaacaa ttatttattt aaattcattt
     7441 agttaattta actacacatt ccattaaatc tttccccact caaaatcaac aacaacttcc
     7501 ctggcaaaaa gtcaaaaaat ggtcattacc ttcctgaagg aatagtgtca ggattaatca
     7561 aaaccaatca catgttgatt cgaattgaac tatcatgaag gtaaatggcg atgagtaaag
     7621 tgaaaagtat cacccgagaa tcctggatcc tgagcacttt cccggagtgg ggtagctggc
     7681 tgaatgaaga aattgaacaa gagcaggtcg cccctggcac atttgcgatg tggtggctag
     7741 gctgcaccgg gatctggttg aaatcagaag gtggcaccaa cgtttgcgtt gatttctggt
     7801 gcggcactgg caaacaaagt cacggtaacc cgttaatgaa acagggtcac cagatgcagc
     7861 gcatggctgg cgtgaaaaaa ctgcagccaa acctacgcac caccccgttt gttcttgatc
     7921 cgtttgcgat tcgccagatc gacgcggtac tggcgactca cgatcacaac gatcatatcg
     7981 acgttaacgt cgctgctgcc gtgatgcaga actgtgctga tgacgtacca ttcattggac
     8041 cgaaaacctg cgttgatttg tggattggct ggggcgtacc gaaagagcgt tgcatcgtgg
     8101 tcaaaccggg cgatgttgta aaagtgaaag acattgaaat tcatgcgctt gacgctttcg
     8161 accgtactgc actgatcacc ctgcctgccg atcaaaaagc ggctggcgta ctgccagatg
     8221 gcatggacga tcgcgcagta aactacctgt tcaaaacccc tggcggttcc ctatatcaca
     8281 gtggcgattc ccactactct aactattatg cgaagcacgg taacgaacat cagatcgatg
     8341 tggcgttagg atcgtatggt gagaacccgc gcggtattac cgacaaaatg accagcgccg
     8401 atatgctgcg tatgggtgaa gccctgaatg cgaaagtggt tatcccgttc caccacgaca
     8461 tctggtcaaa cttccaggcc gatccgcaag agatccgcgt gctgtgggag atcaaaaaag
     8521 atcgtctgaa gtatggcttc aagccgttta tctggcaggt tggcggcaaa tttacctggc
     8581 cgctggataa agacaacttc gagtaccact atccgcgcgg tttcgatgat tgcttcacta
     8641 ttgaaccgga tctgccgttc aagtcattcc tgtaatctgt cgtttatacc ggatagcgtg
     8701 gagaagttcg cgctatccgg cgtagtattt cctggctatt tcaaatatca tctaaaaaaa
     8761 tcaaatttta tcggaatagc tcatgactga agcacaaaga catcaaatcc tcctggaaat
     8821 gctcgcacaa ttgggctttg tgaccgttga gaaagtcgtt gagcgtctgg gaatttcgcc
     8881 tgccactgcg cgacgcgata tcaataaact tggcgaaagc ggcaaactga aaaaggtgcg
     8941 caatggcgca gaagctatta cccaacagcg cccgcgctgg acgccgatga atctgcatca
     9001 ggcgcagaat cacgatgaaa aagtacgtat tgctaaagcg gcctcgcagc tggttaatcc
     9061 gggcgaaagc gtagtcatca actgcggctc caccgcgttt ctgcttgggc gggaaatgtg
     9121 tggcaagcca gtgcaaatca tcactaatta tctaccgctg gcaaattacc tgatcgatca
     9181 agaacatgac agcgtgatca ttatgggtgg acagtacaac aaaagtcagt ccatcacttt
     9241 aagcccgcag ggcagcgaaa acagtctcta tgccgggcac tggatgttta ccagcggaaa
     9301 agggctgacc gcagaagggt tgtataaaac cgatatgctg acagcaatgg cagagcagaa
     9361 gatgctgagc gtggtaggga aactggtggt actggttgat agcagtaaga ttggcgaacg
     9421 cgcgggaatg ctttttagcc gtgccgatca aatcgatatg ctgatcaccg gcaaaaatgc
     9481 taacccggaa atcctgcaac aactggaagc gcaaggggtc agcattctgc gtgtttaaag
     9541 atgctggcgg aaaaatgtca cggcagcatc taacgcctca ggcgtaatgc ggtgacgcac
     9601 gcctggctgc catgaacagg ttagcagttt atcccgtccc gtctcgctta aggcctgctg
     9661 caaacgtagt gattcgtcgg caggcacaac gtcatcgtcg aggccatgcc acagcagtag
     9721 aggtctgtcc ccaagttgtt ccaggtggtt tgtcgcttcc cactctgcca gcggcgcgac
     9781 aattttattg aattcattct gctgtgctgc cgtttcaggt atcagcggtg gaaacagtga
     9841 acgggcgagc gatgtaaaat agcccgatcc catcatgctg gcggtacatt tcaccgtggg
     9901 gtggcgagcg gtaattccca gtgccgtcat cgcgcccatc gacgcgccac cgactgccag
     9961 acggtcatca agcagccagt tttcttcggc tattgccgca cgtaaagtag tgaattcctg
    10021 catactttgt agcaagattt gccagaattg atttaaccgc cgcgcttcgt cgccactaaa
    10081 acggctaccg tgatcgggcg catccggcat gatcacccgc aaaccagcct gcgccagcgc
    10141 aacggcaaaa tagctataca ccaggctgga tgaagtaaag ccgtgataaa aaattacgca
    10201 cggtaacggg gtatcttttt gcccgacagg ataagcatga agaacgggaa tatctgccag
    10261 ctcgcgtgat tctatttcaa tcatctgtct ctcctttttg tctggtatga catgaagaaa
    10321 tccttagcac aagtttctgc tggcggtatt agttcataat gttgagatgt gggttacgct
    10381 ttcgtcacgt tttcattcga aaatcgcgta ccaggtaaca attcgggaac attcccccaa
    10441 agttaaatta ataaggcact acactatggt tagcaggaaa cgtaatagcg ttatttaccg
    10501 gtttgccagt ttattattgg tgttgatgtt aagtgcctgt agcgcactgc aaggtacgcc
    10561 acagccagca ccgccagtaa cggaccatcc gcaagagatt cgccgcgacc agacgcaagg
    10621 gttacagcga ataggtagcg taagcacgat ggttcggggt tctccggatg acgcattagc
    10681 agaaatcaga gcaaaagctg tcgctgcaaa agctgattat tacgttgtcg tcatggtgga
    10741 cgaaaccatc gtgacaggac agtggtattc acaagccatt ttgtaccgta aataaatcag
    10801 acaacttaca tagtcagctt tacattgctt tgcgtcccta tgcgtttacc tgtgcacaat
    10861 aaattaccgg ctggaagctt gggcgtttat taccgcgatg gaatgccctg caacatgtgg
    10921 ggaaacgaaa aatggagctg acgatgaaac aattacttgc ctcaccctcg ctgcaattag
    10981 tgacttatcc tgcgagcgcc acggcacagt ctgcagaatt cgccagtgct gactgcgtga
    11041 cgggcctgaa tgaaataggt cagatctcag ttagcaatat ttcaggggat ccgcaggacg
    11101 tggaacgtat cgtggcgtta aaggccgatg aacaaggtgc gtcatggtac cgcattatta
    11161 ccatgtacga agaccagcag cctgataact ggcgcgtaca ggcgatactg tatgcatgag
    11221 tgtcatctac agcaaatgaa caatgccgga tgcggcgcga gcgccttgtc cggcctacaa
    11281 ttttcacata tttcatttag ttaatcgaaa ccagcgtcgc atcagtcgta tacgcttaca
    11341 cacacactcc ccccgtcgcc cgcagcaata aatcattctg gatctgctct gacaaccgta
    11401 cgccgccgcg cgtatctaac atcacctgac accacgcctg cgccattggc ggagaagcat
    11461 atttcaacat ttgcgcaccg cagcccagca ggaatagctg atgagtaatc tctcgcccca
    11521 gttcttcagc tggcttacgc agctgctgct gtaaacgacg aaccgcgcga tcaaaatagc
    11581 gatcctgccc tttcacttcc acaaatgctt ccgacaataa gtcgtatacg cccgcttgct
    11641 tattgagaac gcgcaacaca tccaggcaca taatattgcc gg
//
LOCUS       DAZSIO010000126        11328 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZSIO010000126 DAZSIO010000000
VERSION     DAZSIO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.126"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            376..815
                     /gene="ycgY"
                     /locus_tag="ACHX5T_003049"
                     /pseudo
     CDS             376..815
                     /gene="ycgY"
                     /locus_tag="ACHX5T_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
     gene            complement(894..2591)
                     /gene="treA"
                     /locus_tag="ACHX5T_003050"
     CDS             complement(894..2591)
                     /gene="treA"
                     /locus_tag="ACHX5T_003050"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR8468139.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(2911..4329)
                     /gene="dhaM"
                     /locus_tag="ACHX5T_003051"
     CDS             complement(2911..4329)
                     /gene="dhaM"
                     /locus_tag="ACHX5T_003051"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFR8468140.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(4340..4972)
                     /gene="dhaL"
                     /locus_tag="ACHX5T_003052"
     CDS             complement(4340..4972)
                     /gene="dhaL"
                     /locus_tag="ACHX5T_003052"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFR8468141.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(4983..6053)
                     /gene="dhaK"
                     /locus_tag="ACHX5T_003053"
     CDS             complement(4983..6053)
                     /gene="dhaK"
                     /locus_tag="ACHX5T_003053"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFR8468142.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6281..8200
                     /gene="dhaR"
                     /locus_tag="ACHX5T_003054"
     CDS             6281..8200
                     /gene="dhaR"
                     /locus_tag="ACHX5T_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFR8468143.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(8300..11167)
                     /locus_tag="ACHX5T_003055"
     CDS             complement(8300..11167)
                     /locus_tag="ACHX5T_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR8468144.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
BASE COUNT         2722 a         2794 c         2837 g         2975 t
ORIGIN      
        1 cattttatta atgcatttct cattcgggtg aaatttccgc tacctggacg cataaaaccg
       61 gaactctcgc caaaagtcgg gcactccgaa ggaattgacg cgttgcgctg ctctgtaacg
      121 gcatttaccg caagccattt atttcgccag cgacaatccg cagagaacca aacgcatcag
      181 gaaacgactg aacacaataa atactgtatt gatcaacata ttatactggc acctccagca
      241 cgcagtgagg atacactgta tcatcagcga atcttatcac tgcggagctg gttgaacgac
      301 tttctgagcg aaataagtcg gttcccggta acaacaacag aatccttttt taattattgt
      361 ttcgttgttt ttggtgtgat tcagaaaata aatattttag acagtagcca gtcaggcgtt
      421 aaaaatgggg cggatagtga ggcggttttg gctgcgctta ttgaacattt agaattgatc
      481 aatccttctg gcagattaag ccaaaatacc cgttcggcaa tgcttcagtt acgcgaagag
      541 gaatggtctg aattcttttt ttggttgcta aattctctgg agtgtctgga ttatgtgatt
      601 attaatttaa ctccagaaag caaaaaaacg ttgatgagtg aacaccgtaa caatattcgg
      661 tagcaattga tgctttatat agccaacgtc ggcgtaaatc gccgggtgat gaaagtgaaa
      721 cacttacccg tagaaatgac gctatcttcg gcaaccacgt atggcaaaca tttgcgcaat
      781 acttcccgcc aggacttgaa aaaccgtctg tgtagaaaaa accatagata tggtgttatc
      841 tgccgacaac gccatccgga gaagccgaat gacgttgtcg gagtaagcgc tggttaaggt
      901 gtgggttgtg cctctttggt tgagggttgc gtcgttgctg acttaacggt cggatgcgtc
      961 gccggaacat tgtcacacgg ttgctctttc gggcagatca aatccagcat tttcagcgtc
     1021 acgccattgg tccagccaaa gccatcctgt aatggatatt cgccaccgcc gccccccgtt
     1081 ccggtggtgc tgacatcata tttttccacc agctttttct cccggtcata ggtgtgctga
     1141 acattggtca ggaagtgcca gctaatgtcc atcgccacct ctttttgccc gtagttttgt
     1201 aatccttctg tcgcgaccca ctgtaacggt gcccagccat ttggcgcatc ccattgttgc
     1261 ccacttttca ccgacgtggt gttcaggccg ccgggttgca gcagatgtgt tttcgtcgcc
     1321 gtcgccattt tgttggcgcg atctttcgct gccgcattga cgtacagcgg gaacagggcg
     1381 gccgcggtta actgattgcg cactttatga cttttcaggt cgtaatcggc ataccagcct
     1441 tgttgatcgt tccacaggta tttttcgatc cctttttgac gggcattcgc cagcgtttcg
     1501 tactggtttg ccatcgcgtt atctcctgca gctttgctgg cgcgggcgag gattttttcc
     1561 attttaaaca tcaggctgtt cagatcgacc ggtacgatgc tggtggtgcg taaggtattt
     1621 aactgctgcg ggttgtccat ccagcgcgag ctgaaatccc agccagacgc agcggcagag
     1681 cgcaggtcgc ggtaaatttc agtggcaggt cgattcggat tgcttttggc ggtggcaata
     1741 tcttccaccc atgactctgg tcgtggcgta tcgcgatcgt cccagtagcg gttgagaagg
     1801 gtactatcct gaagttttac aacgcgtttt tcctgttgtc cggcttgcag gttttcaaca
     1861 ccgtccatcc agtaagcata ttctttttgc atttgcggca ggtattgctt caacgcggca
     1921 tcgccttcat gctgcgccag taactctacc atcagggcaa agaagggcgg ttgcgagcgg
     1981 cttaaatagt aactgcggtt gccgttggga atatgaccgt aagtgtttat ttcatgagca
     2041 aaattggcca ccatatccgc gattttatcc cagtgaccac tttcggcaag tcctaacatg
     2101 gtgaagtaat tgtcccagta atatacctcg cgaaaacgtc cgcccggcac gacataaggt
     2161 tccggcagcg gtaacagaga atcccatttt tcggtgtttt cggtagaacg cgttaatacc
     2221 ggccaaagtc cgtcaatatg ttcgcgcagt gactgcccct ctggcggaac atatttctcg
     2281 ccttctttcg gcagggtgaa attgacgtta acgaaatggc gcagatcaaa tccgctctgg
     2341 ttttgctgca tccgataatc agcaaggatc atcagcggat cgctgttagg cacggcatcg
     2401 gcaaaggttt tttggtccgg aaaaagtttg acgttttgca catcattaaa cagcggccct
     2461 aataaaatat caggcggctg tggtgttacc ggtgtttctt ctgcctgcac cgatagcgca
     2521 gcgaaacaca aaaagataca ggctggaatt aacgccattt tttgcgggcg agaaggtgcg
     2581 ggggatttca tcaatcattc tcctttggcg aaaccgaata aaacggtatt caagtatcag
     2641 aaaaccttag ttcaggatcg cactagctgc atgatcgaaa tctcattctg cgaacggtca
     2701 ggcattttag aaaagggaat taaagggata aataacggcg aaatagagtg atcaaataac
     2761 gtctgtttat tagccagcca gcgacatttg gttcgcgatt agcgaagaaa aatcatcata
     2821 aattggtaaa aaatataaca ggtagttaaa aaacatcagt gctgggtaaa ttgccggatg
     2881 acatcagaac gatgccatcc gaacggtggc ttaaccctga cggttgaaac gttgcgtttt
     2941 aacgtccagc gttagcgttt cttctggttg tatcgcatac agtttctcac cctgctggca
     3001 aatccagcca atccccagtt cacgggcgat tagggcgctg tgggataccg gacttccggc
     3061 gctaaggcag atacctttta caaccgccgg atccagttgc agtactgtgg aaggataaat
     3121 gttctccgcc agtagaatag ttggcgagtt aaactgcggg agttcttctt tcgtttgggt
     3181 caggtggacc agggtgcgat gcagaagatc gtccacatca atatagcgag cttgtagata
     3241 ttcatcatcc agttgctggt attgctggct aagttcttta agaacttgct gccaggcata
     3301 ttctgccgtg caatgttcat gctgaaggag ttcgcttgcc gccgccagca gttccggatc
     3361 acctaacagt gtatggtgac cagaaaagat tgcggcaata tcgtcaagcc cgctggcttc
     3421 tgctttcgct gttaacgtca tcagatctaa taacgtgaag tcaatagcct ggcgtaatcg
     3481 atcttgttct tcttccgcgg tcagggttga ttttgcctgt accgtacata aaactggttg
     3541 ataataaaag gctttacccg aaacaggcag aacgggacgc agagtaggtg gagcgacttc
     3601 ctccgtttca ccaaagttat cttcagccag gtgacggaaa gcgatcagtg cctcttcagc
     3661 ttctggccct ttcgcaatca ggcgcagcgt atcgttatag cgaacttgta gtaacgcaat
     3721 ctggttaata ctctctggtg tgacgcattt gccgtttttt tccagcaaca tatcggcatt
     3781 aaatgtcgat aaggtataaa ccagccggga ggccggacgt acatgcaggc cgttacggtt
     3841 ttttatgacc accgccagag aacgggcttc ttcatcgtac ggaggacatg tgtcagagat
     3901 ttcagtgtcg gaggacggta aacccagttg ttcacgtttg gcttccagcg catgcatagc
     3961 gtcaaagata actttgtcga tatccgcccc cgaggccgcg ctgaccgttg ctgccagtgt
     4021 accttcgacc aacggcgcag cacacaaacg tacttttgcg gcgatctcgg gagccagcaa
     4081 ttccagcgca gtttcagcac tcaataatgc gctacccata tccatcatga ccagcacatg
     4141 gtcggcatca gcaacagatt cgatggcctc catcactttg acggcatcgg taccaatggg
     4201 attttgtgga tcgtcaattc ccgcggcaat ggcgatttta caactatcac tcattaacat
     4261 ctgacgggct aattcaccga caccttctcc cagtcggctg ctatgtgaaa ctatgaccag
     4321 gtttaccatc accaattcct tactcttttg cggctaacgc caacatttgc atcataaaca
     4381 tcaccgaggt cgcgccggga tcctggtgac caatactgcg ttcaccgaga taactggcgc
     4441 ggcctttgcg ggcttgcatc gtaatcgtac tttgtgcagc ggattcggcg atgctactgg
     4501 cagcttcgag cgccaccgga acagagagat tttgctcgct ggactgacgt aacgattcca
     4561 ccaccggcac ccacacatca cacatggttt tatcgccagg ttcggctttc ccgcgactga
     4621 ttacgccgtc cgcgccatcg cggaacatct gataaagctc ttccagtgtc aggctttgcc
     4681 gtgcctgggt cgcctgtgcg gcgcggataa agaaggtacc gaacagcgga ccactggcac
     4741 caccgacgct ggaaagcagc gtcataccgg tatttttgag aatgaaaccg atatctttat
     4801 ctgcgatagc agggagtttt tccaccactt tgctaaagcc tcggttcata tttagcccgt
     4861 ggtcagcgtc gccaatctcg cgatccagcc cggtaagata ctcgctctcg gtgttgaaaa
     4921 tatcgccaca acgagtgagc cagttaacaa tttgagttct gctcagtgac attgctttct
     4981 ccttatttac cccagttaag ggccggggtg tggaccgggg cgtcccagag agccagcgtt
     5041 tcgtcatcaa ctttcagtaa ggtgattgag aaaccggtca tatccagtga ggtgcagtac
     5101 gcgccaatta aattacgttc gatagtcaat cccgcttgct ggcaacgtgt ggtcaggcgg
     5161 ttatagacgc cgtacagctc agaaagcgga gttgcgccaa gattgttaac cagtgcaatc
     5221 acccgatcgc cagactggag cggttgtttg gtttgtggtt cttcctgcca gctgccttgt
     5281 tgataatccc agaaacgcaa agtgcgatgg tatgagccat gttccagcag ggtgtcgaac
     5341 atttcatcga cggtttgatc aagggaagag aaagggcggc ggtcaatacc tggctcacca
     5401 tgaatgccta cgccaaactc catctcatta tccgcaaggg taaaagaagg tttgcccgcg
     5461 gcaggaacgg tacaggcacc gagagcgata cctattgagt ggccttgatt attcaactta
     5521 cgccccagtt ccgcacaggc gtccagtgag tcgccacgct ccgccgctgc gcctacgagt
     5581 ttttcaatta atacggtgtt ggcaacgccg cgtcgcccgg cggtataaag actgtctttt
     5641 accgcaacgt cgtcatcaat gaccacagtg gtcactttta cgccgctatc gtgcagtaac
     5701 tcggtcgctg tttcaaagtt aagaatatcg ccggtgtaat ttttgataat caacagtaca
     5761 ccttcgccgc catcaatttg catggagcat tcaaagattt tatcgggcgt cggtgaggtg
     5821 aaaatttcgc ccggacaggc ccccgaaagc atcccctgac cgatataacc acagtgcatc
     5881 ggctcgtgtc cgctgccgcc acccgacagc agggcgactt ttcctgcaac aggggcatca
     5941 gctcgggtga catacaccgg atcctgatgc agtgtcagcg atggatgcgc tttcgccagt
     6001 cctgccagtt gttcgtccag tacgtcttgc acatcattga tcaatttttt cattattttg
     6061 ctccagcaat tacggtaggg catggatgat gttcaacgac acggcgacca ctgactgccg
     6121 atgaatccat tgtgcatcag gcaagggaaa agaaaattag cccaaattat gtttcatagt
     6181 gaaacatatg ctttaatgaa tgttccatat tgaaactttt acgtgtatta atacttaaaa
     6241 ttgcgagccg gaacaccttt tgtcataagg gatgcgggat atgagtggcg cttttaacaa
     6301 cgatggtcgg ggcatatctc ccttaattgc aacctcctgg gagcgatgca ataagctgat
     6361 gaaacgggag acatggaacg taccacatca ggcccagggc gtgacatttg cttctattta
     6421 tcggcgtaag aaagcgatgc tgacgctcgg gcaggctgcg ctggaagatg cctgggaata
     6481 tatggcaccg cgagagtgtg cgctgtttat cctcgatgaa accgcctgca ttctcagccg
     6541 taatggcgat ccgcaaacct tgcagcagct aagtgcactg ggattcaatg acggcacgta
     6601 ttgcgccgag ggaattattg gtacttgtgc gctatcgtta gcggctatct ctggtcaggc
     6661 cgtgaaaacg atggccgatc aacatttcaa acaggcactc tggaactggg ccttttgtgc
     6721 aacgccgttg tttgacagca agggccgatt gacgggaaca atagcgctgg cgtgtccggt
     6781 tgagcaaact accgcagctg atttgccgtt gacgttggca atcgcccgcg aggtcggtaa
     6841 tttactgctg acggacagtt tgctcgctga aactaaccgt catttaaatc aacttaatgc
     6901 cctgttagaa agtatggatg atggcgtgat tagctgggac gagcagggta atttgcaatt
     6961 tattaatgcc caggcggcgc gggtcttgcg ccttgacgcg acggcaagtc agggacgggc
     7021 aatcactgaa ctcttaacgt tacccgccgt attgcaacaa gcaataaaac tggcacatcc
     7081 gctcaaacac gtagaagcaa cctttgaaag ccagcaccag tttattgatg cggtgataac
     7141 ccttaaaccg ataatagaaa cgcagggaac cagctttatt ttgttgctcc atcctgtgga
     7201 acagatgcgg cagttaatga ccagtcaatt aggaaaagtc agccatacct tcgctcatat
     7261 gccacaggac gatccgcaaa cccgccgctt gattcatttt ggtcgccagg cggcgcgcag
     7321 tagctttcct gtcctgcttt gtggagaaga gggcgtgggc aaggcactgc taagtcaggc
     7381 aattcataat gaaagcgagc gtgctgcagg tccttatatc gccgtcaatt gtgagttata
     7441 tggtgatgct gcgctggcgg aagaatttat tggtggcgat cgcacggaca atgaaaatgg
     7501 ccgtctgagt cggctggaac tggcacacgg cggcacgctg tttcttgaaa agattgaata
     7561 tctggcggtg gagttacagt ctgctttgct gcaggttatc aagcaggggg ttatcacgcg
     7621 actggatgcg cggcgtttaa taccaattga tgtcaaagtg attgcaacaa cgaccgcgga
     7681 cctcgcaatg ctggtggaac aaaatcgttt tagtcgccag ctgtattacg cgctgcatgc
     7741 atttgaaatt accatcccgc ctctgcgtat gcggcgtggc agcattccgg cgctggtgaa
     7801 taacaaatta cgcagtcttg aaaaacgctt ctctacgcgg ctgaaaattg atgacgatgc
     7861 cctcgctcgc ctagtttctt gtgcatggcc aggcaacgat tttgaacttt acagcgtcat
     7921 cgagaatctt gctctgagta gtgataacgg gcgcattcgc gtcagtgatt tacctgaaca
     7981 tctgtttacc gagcaggcga cagatgatgt cagcgccacc cgcctttcca ccagtctgtc
     8041 atttgcggaa gttgaaaaag aggcaattat taacgcagcc caggtcacag gcggtcgcat
     8101 tcaggaaatg tcggctttac ttgggatcgg ccgcactacg ctgtggcgga aaatgaagca
     8161 acatggcatt gatgcagggc agtttaagcg ccgggtatga aagacagaaa cgatttctga
     8221 tacatcagag tgatctgtat ttcattccgg cgcacgctaa caattttcag catcgtttaa
     8281 gggcttgtct atcccgcact taaaagctga agcgatatcc tccgttgact tgcttctgat
     8341 caaagaggtt accctgcgtg taatccgctt cgagatagaa tgtgtgctgt ttgttatact
     8401 gtgcactgac tcccacgccg ttattccagc cattaccttt gaaactgtac ttctcccggg
     8461 agtcgttcaa cagatattcg gtatcccctg aaaactcgcg gatagcgcca gtcttcacat
     8521 agacattcag ctggctgttg cctgcggtga tgtcataccc cagtatcatg ctggcacgcc
     8581 ccagcagcga ttcgtagtga ttcagatgta tattgaggcc attactcgcc ttcatagcca
     8641 tctcattctg gtggctgtat gtaagctggg tttgcggctc tatatagaac ccataaccag
     8701 taggggacag gttgaacctc tgcccggctt ccagggagat gctcattcca ttcgcagtgc
     8761 cgttggcgtt aacgccgttg ttctgactgt ccagtacgtg gaaactattt ttctggcgcg
     8821 atgcttttat aacgagatcg ctgtaaaaac cgttttgtgc catgtaactg gcgtacattc
     8881 ccatgtagtc tgaacgtgcg gtaccgtcgc ctccgctata gtccggcgat gcatgtgttg
     8941 agtcaatata cagaccgaca tacaacggca ttacatcaga gagacgttta tccccaccaa
     9001 actggatacc gctgtaaccc atgtcaaagc cgctcagttt gccactggca aaggagtcca
     9061 ggcttccccc ataactgcgc aaccagatat taccgtcttt actctgattt cgcaagtcac
     9121 ccatccgttg catcagcgta cggttttcaa catagttcaa taagtaaccg acattgagat
     9181 aattgccgcc agcatctgcg gtcgtggtgg gtttaggcgt gggagcgggt tcaggcgtag
     9241 gatcggggtt gactatggga ggctgagcgg gagctggtgt gggttcagga ttaggagtcg
     9301 gttccggaac tgtcccggaa gcgtaaagct cccagttagt gccattttta cgcacatcgt
     9361 acagatatcc ccccaactca acctgagaag acgcgctgaa cgaggccgcg ccgtcagtgg
     9421 tttttaccac tgtcagaact tcatttcccg ttgtggcctc gctgccctgg ttgcggatag
     9481 ccaatacgtg attaccagca ctactcccgc tgatattcaa taaatcccct ctattattaa
     9541 cgccattacc ctcgccaaca acatcagcac gcataataaa ggtactgtta ccgctcaggt
     9601 tctctacgtt taatgtggta aatgtgccgg cagttgaccc gtggctggca aaatcgacag
     9661 ttgaatggct cagcgccagc gtgtcgagat tagaattact tgttacgttc cagacgctgt
     9721 tattcattgc aacgtccagt ttcccgccat tgacattatc gctgagggag gaacctgtcc
     9781 aaaccgaccc agggtgcata tccagattga ttagcccacc tttggaaaga acgctaccgt
     9841 tgataagcat acgacccgag gcgttgatgc ggctggcggc ataaccatcg tcatgttgcg
     9901 ttgcgatggc catctggtcg ggtgtactca tatcaatgac cagatcgctc gtgaggtcga
     9961 tctggctgtt ggtcatggca taaatccctc tggctcctgc cgcgccggtg atagccaaac
    10021 tgtctccggt tattctaccg ccgctgagcg cccacaaacc cagcgccaga ctgccattac
    10081 gatcaaccgt aatatcggta ttttgcatgt tgataacagc cgttgccgtc tgggccgagg
    10141 caccataaga accgccggaa aagatgctgt ttcgttgcgc tgccgtgcca gaaaaattga
    10201 ttgtcgcgtc tgaactactg gtgacgaggc caccgccctg cgcggaagaa atatggctat
    10261 ctgcaccgat agtggttgta ccaccacgaa cttcgacgcc attggccgcg gctccagtta
    10321 catcaacagt gagtgcattc gcgctaacct ggccaaagct ccagaggccg tgagcattat
    10381 cgccattggt tttaatggta ctgtttgttc cgagatcgac aacagagttt ttctgtacgt
    10441 ttatccccat ggcgctgtag ccctgaacat cgattgtcag gtctgtcgcc gtaaaacgcg
    10501 cagcaccatt ggcgttattg ccgttgagac caccgatata aacacctgta cttccatcgg
    10561 tcttgatttt acttccgctt ccaagatcga cactggtgcc atagtcattg atggttaggc
    10621 ctataccgtt cgagttttca atggtgaatt gagtggctgt tagtgttgag ctatgcccaa
    10681 taatgatgcc gtcatcgtta ctcttaatgg tgctgcctgt gcctaagtca gcatgggtat
    10741 agtcaccaat taagttaatg ccgatggcag aggtttgacc aacaacatct actgtgagtc
    10801 ggttagctgt cagggagctg tttttgccct gaataatgat tccttttgca tatccagagg
    10861 cgtcgttgac attgacggtg acatcattac ccaggttgat attgccaggc gtgtttcctg
    10921 ctggggtcat tacaccatag aggtttcctg atggatcggc ggtatctcct gtaattctgt
    10981 caccatcatt aatagtaata ttggtgccat attgacttcc tgtatcagca gctatcagca
    11041 ggggggaaaa gataattgca ttcaaaccaa tagcgccaaa ttttatcagt tgaattgagg
    11101 gcgaacggat ttttaattca gcgagacgat cggctttggt attcccattg tgttgtttga
    11161 tgcccatacc actcctatat agtacccagg ctatgggtct atggattttg tcattttgtt
    11221 taagtcaatg aataatatcc tcttatcatc atgatatctg tatggtgcaa tcatgatgag
    11281 cttctctgag aataattctc tattaccggg caaaatcagg aacacagg
//
LOCUS       DAZSIO010000127        11317 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZSIO010000127 DAZSIO010000000
VERSION     DAZSIO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.127"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="ACHX5T_003056"
     CDS             complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="ACHX5T_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR8468145.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            321..1952
                     /locus_tag="ACHX5T_003057"
     CDS             321..1952
                     /locus_tag="ACHX5T_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR8468146.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1949..2668
                     /gene="dcuR"
                     /locus_tag="ACHX5T_003058"
     CDS             1949..2668
                     /gene="dcuR"
                     /locus_tag="ACHX5T_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR8468147.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3239..4579
                     /gene="dcuB"
                     /locus_tag="ACHX5T_003059"
     CDS             3239..4579
                     /gene="dcuB"
                     /locus_tag="ACHX5T_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR8468148.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4657..6303
                     /gene="fumB"
                     /locus_tag="ACHX5T_003060"
     CDS             4657..6303
                     /gene="fumB"
                     /locus_tag="ACHX5T_003060"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR8468149.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6426..7055
                     /locus_tag="ACHX5T_003061"
     CDS             6426..7055
                     /locus_tag="ACHX5T_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR8468150.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7194..8615)
                     /gene="melB"
                     /locus_tag="ACHX5T_003062"
     CDS             complement(7194..8615)
                     /gene="melB"
                     /locus_tag="ACHX5T_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFR8468151.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8718..10073)
                     /gene="melA"
                     /locus_tag="ACHX5T_003063"
     CDS             complement(8718..10073)
                     /gene="melA"
                     /locus_tag="ACHX5T_003063"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR8468152.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10356..11264
                     /gene="melR"
                     /locus_tag="ACHX5T_003064"
     CDS             10356..11264
                     /gene="melR"
                     /locus_tag="ACHX5T_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR8468153.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
BASE COUNT         2958 a         2911 c         2748 g         2700 t
ORIGIN      
        1 gactttcgtt tgagattaaa taacttgccg ttgccacgta cgcaattgcc agaatgctga
       61 catatcgcag tgttgaaata gacatcgatc ttttcgccgg tataacagcg ataacccccg
      121 tccagtagcg cctgatccat gaccttccct ctctcatcag tatgattatc gctataagca
      181 tagccccgga atcttcatac agcactggca gtttccgttg ccagagtctc ttcccctata
      241 tagccaatca tttttactcc ctttgaatta cccgcctcat cagagataat gcttaagaaa
      301 tttgtcacac aaggaagctg atgagacatt cattgcccta ccacatatta cgcaaacgtc
      361 cgatgaaatt gagtaccact gtgatcttaa tggtcagcgc ggtactgttc tcggtgctat
      421 tggtggtgca tctgatttac ttctcgcaaa tcagtgatat gacgcgagac gggctggcca
      481 acaaggcact ggcagtggcg cgtaccctcg ccgactcgcc ggaaatccgt cagggcttgc
      541 agaaaaaacc gcaggagagt ggcatccagg ccatcgcgga agccgtgcgc aaacgcaacg
      601 atctgctgtt tattgtcgtt accgatatgc aaagtcttcg ctactcacat cctgaagccc
      661 agcgtattgg tcagccattt aaaggtgatg acatccttaa tgcgctgaat ggcgaagaaa
      721 atgtcgctat caatcgcggt tttctggcgc aggctttacg cgtatttacc cccatctacg
      781 atgaaaatca taaacaaatt ggcgtggtgg cgatcggcct tgagttaagc cgcgtgaccc
      841 aacagatcaa tgacagtcgc tggagcatta tctggtcggt attatttggc atgctggtcg
      901 gactgattgg cacctgcatt ctggttaagg tactgaaaaa aatccttttc ggcctggaac
      961 cctacgaaat ctccacgctg tttgagcaac gccaggccat gttgcagtct atcaaagaag
     1021 gcgtcgttgc cgtggacgat cgcggcgagg tcacgctgat caacgatgcc gcacaagaat
     1081 tgctgaatta ccgtaagtcg caggacgatg agaaactgtc gacgctcagc cactcatggt
     1141 cacaggtggt agatgtctcg gaagtgttac gcgacggtac gccgcgccgc gacgaagaga
     1201 ttacgattaa agaccggcta ttactgatca acaccgttcc ggtgcgcagt aatggcgtta
     1261 tcatcggtgc catttcaacc ttcagggaca aaactgaagt tcgtaagctg atgcagcgac
     1321 tcgacggtct ggtcaactat gctgacgcac ttcgtgaacg atcccacgaa tttatgaata
     1381 aattgcatgt gattctcgga ttattgcatc tgaagagtta taagcagttg gaagattaca
     1441 ttctcaaaac agccaataac tatcaggaag agattggctc tctgctgggt aagatcaaat
     1501 ctccggttat cgctggtttt ttaatcagca agattaaccg cgcgaccgat ttaggccata
     1561 cgctgatttt aaacagtgaa agccagctgc cagacagcgg cagtgaggac caggtcgcga
     1621 cgctgattac cacattggga aatctgatag aaaacgcgct ggaagcatta gggccggaac
     1681 ccggtggcga aattagcgta acattgcact accgtcacgg ctggctgcac tgcgaagtta
     1741 acgatgatgg accggggatc gcacccgaca aaatcgatca catttttgac aaaggtgtct
     1801 cgacaaaagg aagcgagcga ggcgtcggtt tagcacttgt caaacaacag gtagaaaatc
     1861 tcggcggcag catcgccgtg gaatcggaac cggggatttt cacacaattt tttgtccaga
     1921 taccctggga cggggagagg tcgaacagat gatcaatgta ttaattatcg atgacgacgc
     1981 aatggtcgcg gagctgaatc gccgatacgt agcacaaatc ccaggctttc aatgctgtgg
     2041 aacagcctcg acgctggaga aagccaaaga gattatcttc aatagcgatg cgcctatcga
     2101 cctgatattg ctcgatatct atatgcaaaa agagaacggg ctcgatttac tgcctgtcct
     2161 gcataacgcg cgttgcaaaa gtgatgtgat tgtcatctcc tccgcagccg atgcggcaac
     2221 cattaaagat tcgctgcatt acggtgtcgt ggattacctg atcaaaccct tccaggcttc
     2281 ccgctttgaa gaggcgctca ccggctggcg gcaaaagaaa atggcgctgg aaaaacatca
     2341 gtattatgac caggccgagc ttgatcagct aatccacggc agcagctcca acgaacagga
     2401 tcctcgtcgc ttgccaaaag gcttaacgcc gcagacgtta cgcacgctgt gccagtggat
     2461 tgacgcgcat caggactatg agttttcaac cgacgagctg gctaacgagg ttaacatctc
     2521 gcgtgtttcc tgccgaaaat acctcatctg gctggtcaac tgccacatct tgttcaccag
     2581 tatccattat ggcgtcacgg ggcgtccggt ttaccgttac cgtattcagg cagaacacta
     2641 ctcactgctg aaacaatatt gccaataacg gatcgctggt tatctgtaag taataattat
     2701 taccgtaatg caaaataccc ctggcacaag ttacatcgtt aatcagtaaa acttgtgcca
     2761 gatcaaatat aattatccct ccatcatcgt taaaaattaa tatctcttca gatgaacggt
     2821 gtttttaatt tcaaaacgat aacaaaagtt aattaactat tatgtcaccc gcattatgtg
     2881 tatttttacc cacaaatggg tagatcagat taatctataa acctaatgat atctgccctg
     2941 agaacaaaaa atagaccgat aaatatcaat aagataacag caaacaaaac attaacatct
     3001 gcgcagtaca aactataaac ccatcgccag agagtttttc tctctgaaaa agccgcttat
     3061 cacagtgcat aaatttgccg ctgctttaat cagccaatat tcactgtgag gtatttgcta
     3121 aagccggtaa cgaccaaacg gatatttagt caggctctga aaacagttca tacaaaacag
     3181 aacgtgactg tgatctattc agcaaaaatt taaataggat tatcgcgagg gttcacacat
     3241 gttatttact atccaactta tcataatact aatatgtctg ttttatggtg ccagaaaggg
     3301 tggtatcgcg ctgggtttat taggcggtat cggtctggtc attctggtct tcgtcttcca
     3361 ccttcagcca ggtaaaccac cagttgatgt catgctggtt atcattgcgg tggtggcggc
     3421 atcggcgacc ttgcaagctt cgggcggtct tgatgtcatg ctgcaaattg ccgagaagct
     3481 gctgcgccgc aacccgaaat atgtctcaat tgtcgcgccg tttgtgacct gtacactgac
     3541 cattctttgc ggtacgggtc atgtggttta caccattctg ccgatcatct acgacgtcgc
     3601 cattaagaac aacatccgtc cggaacgtcc gatggcggca agttctatcg gtgcacagat
     3661 ggggattatc gccagtccgg tgtcggttgc ggtcgtgtct ctggttgcga tgctgggtaa
     3721 tgtcaccttt gatggtcgcc atcttgagtt cctcgatctg ctggcaatca ccattccatc
     3781 gacgttaatc ggtatcctgg cgatcggtat cttcagctgg ttccgcggta aagatctgga
     3841 taaagacgaa gagttccaga aattcatctc cgtaccggaa aaccgtgagt atgtttacgg
     3901 tgataccgcg acgctgctgg ataaaaaact gccgaaaagc aactggctgg caatgtggat
     3961 tttcctcggg gcaatcgctg tagtcgccct tcttggtgct gattcggacc tgcgtccatc
     4021 cttcggcggc aaaccgctgt cgatggtact ggttattcag atgtttatgc tgctgaccgg
     4081 ggcgctgatt attatcctga ccaaaaccaa tcccgcgtct atctcaaaaa acgaagtctt
     4141 ccgttccggt atgatcgcca tcgtggcggt gtacggtatc gcatggatgg cagaaaccat
     4201 gttcggtgcg catatgtctg aaattcaggg cgtactgggt gaaatggtga aagagtatcc
     4261 gtgggcgtat gccattgttc tgctgctggt ttccaagttt gtaaactctc aggctgcggc
     4321 gctggcggcg attgttccgg tcgcgctggc gatcggcgtt gatccggcat acatcgtggc
     4381 ttcagcaccg gcttgctacg gttattacat cctgccgact tatccgagcg atctggcagc
     4441 gattcagttt gaccgttccg gcaccaccca catcggtcgc ttcgtcatca accacagttt
     4501 tattctgccg gggttgattg gtgtgagcgt atcgtgcgtc ttcggctgga tcttcgccgc
     4561 gatgtacggg ttcttataaa tgcactttgc gtgccgcccg tgactacgcg gcacgccatt
     4621 ttcgaataac aaatacagag ttacaggctg gaagctatgt caaacaaacc ctttatctac
     4681 caggcacctt tcccgatggg gaaagacaat accgaatact atctactcac ttccgattac
     4741 gttagcgttg ccgacttcga cggcgaaacc atcctgaaag tggaaccaga agccctgacc
     4801 ctgctggcgc agcaagcctt tcacgacgct tctttcatgc tacgtccggc acaccagaaa
     4861 caggttgcgg ctattcttca cgatccagaa gccagcgaaa acgacaagta cgtggcactg
     4921 caattcttaa gaaactccga aatcgccgcc aaaggcgtgc tgccgacctg ccaggatacc
     4981 ggcaccgcga tcatcgtcgg taaaaaaggc cagcgcgtgt ggaccggtgg cggtgatgaa
     5041 gaagcgctgt cgaaaggcgt ctataacacc tatatcgaag ataacctgcg ctactcgcaa
     5101 aacgcggcgc tggacatgta caaagaggtc aacaccggca ctaacctgcc tgcgcaaatc
     5161 gacctgtacg cggtagatgg cgatgagtac aaattccttt gcgttgcgaa aggcggcggc
     5221 tctgccaaca aaacgtatct ctaccaggaa accaaagccc tgctgactcc cggcaaactg
     5281 aaaaacttcc tcgtcgagaa aatgcgtacc ctcggtactg cagcctgccc gccgtaccat
     5341 atcgcgtttg tgattggcgg tacgtctgcg gaaaacaacc tgaaaaccgt caaattagca
     5401 agcgctcact attacgatga actgccgacg gaagggaacg aacatggtca ggcgttccgc
     5461 gatgtccagc tggaacagga actgctggaa gaggcccaga aactcggtct tggcgcgcag
     5521 tttggcggta aatacttcgc gcacgacatt cgcgttatcc gtctgccacg tcacggcgca
     5581 tcctgcccgg taggcatggg cgtctcctgc tccgctgacc gtaacattaa agcgaaaatc
     5641 aaccgcgaag gtatctggat cgaaaaactg gaacacaatc taggtcagta cattccacaa
     5701 gaactgcgtc aggccggtga aggcgaagcg gtgaaggtcg accttaaccg cccgatgaaa
     5761 gagatcctcg cccagctttc gcaatatccg gtttccactc gtttgtcgct cactggcacc
     5821 attattgtgg gtcgagatat tgcacacgcc aagctgaaag agctgattga cgccggtaaa
     5881 gaacttccgc agtacatcaa agatcacccg atctactacg cgggtccggc gaaaacccct
     5941 gccggttatc catcaggttc acttggccca accaccgcag gtcgtatgga ctcctatgtg
     6001 gatctgctgc aatcccacgg cggcagcatg atcatgctgg cgaaaggcaa ccgcagccag
     6061 caggttaccg acgcgtgtca taaacacggc ggcttctacc tcggtagcat cggcggtccg
     6121 gcggcggtac tggcgcagca gagcatcaag catctggaat gcgtcgctta tccggagctg
     6181 ggtatggaag ctatctggaa aatcgaagta gaagatttcc cggcgtttat cctggtcgat
     6241 gacaaaggta acgacttctt ccagcaaatc gtcaacaaac agtgcgcgaa ctgcactaag
     6301 taacctcttc ggcccagcgc ctggcagcat gctgccaggt gatccccctg ggccacctct
     6361 tttgcgattg taatttcatg cttgctggtg aatagtcagt attttccctg acttgcgaac
     6421 tcaccatgac ccgcacactc aagccgttaa ttcttaacac tggcgcactg gcgctaacgt
     6481 taatcctgat ttataccggc atttcggccc atgacaaact cacctggctg ctggaagtga
     6541 caccggtgat tatcgtcgtg ccgctactac ttgccaccgc cagacgttac ccgttaacgc
     6601 tgctgctcta tacgctcatc ttctttcacg ccatcatcct gatggtaggc gggcagtaca
     6661 cctacgcgaa agtccccgtt ggttttgagg tgcaggaatg gctggggttg agtcgtaatc
     6721 cgtatgacaa gctggggcac tttttccagg ggctggtgcc tgcactggtg gcacgagaaa
     6781 ttctcgttcg cgggatgtac gtgcgcggac gtaaaatggt ggcgtttctg gtctgttgcg
     6841 tggcgctggc gataagcgcg atgtatgaat taatcgagtg gtgggcggca ctggcgatgg
     6901 gccagggagc ggatgatttt ctcggtaccc agggcgacca gtgggacacg caatccgata
     6961 tgttctgcgc gctgcttggc gcattaacga cagtgatatt gctcgctcgc tttcattgcc
     7021 gccagctacg gcgctatggc ttgataactg gataacaacg tgcactttgt tgatgccgga
     7081 tgcggcgtga attccttatc cggtctacaa aatttgcaaa tccaaagcat tacattaaga
     7141 cgtggcctga taaacatatc gcatcaggcc attttcgtta gctattgtcc acgtcaggct
     7201 ttcacatcac tcactgcacc gaccggaata tcagcatgaa caggctcggg cggtactttg
     7261 cgatatttat ccagcagatg aatctggatc ctgcgcagcg tgtcgccatt gaggcgatag
     7321 aaacggaagt agagaatcag cgttaccatg aaaaacagag tcggtagagc aatcataata
     7381 aactgcatac ctaatagggc ttgcgtagac tgttcaacat tcggtacata gccaatcatc
     7441 cctaacacaa ccgcaatgaa aaaagccgca aaggctgaac cgcccttcac caccatagtc
     7501 tgcacggagt aagcgatgct ttcacagcgt acgtgcagtt tatattcacc gtaatcaacg
     7561 gtatctgcca ccatgatgac ctgtaatacc cagaaaagcg ccgttcccac attcagcaaa
     7621 atacccgcaa tcacaatgag gacgacgttg tgatagctca ttaatgccat taacaggaga
     7681 acaccacagc ttaacaccgg aagaatagat gctccggccc ataaaatgcg tcgggataat
     7741 gatttaacta agcgtgggaa gaatactaac gtcaccaggt tagcagctcc cgcatacgac
     7801 agataatagg ggaacaaatc cgcatcaccg ataacatatg agaaataata gatagcaaat
     7861 ccggtaataa tgttgctggc tacattataa gcaagagcca tacccaagag gcatgaaagc
     7921 tgatcgtttt tataaataag cgcaacgatg gctttaagtg tcagatggct tccttcagca
     7981 gacgattgat tgtctgacga aaagacttca tgcacattgc gcagagtgat gatggttgaa
     8041 acaataaaaa aggcgatcaa taccagagtg aacatctgaa agccaaatcc ccgatcgcca
     8101 ccgccgacat aattaacaaa tggtagcgtc acacctgccg taacaaagcc tgccagactg
     8161 gcaaaaaaac gcggataagg aaccagttgt tcgcgttcac gtttatcgag cgtgatggtt
     8221 ggaaccagcg accagaaggg aatatccata atggtgtaag tcatgcccca gaggatgtag
     8281 gtcacgcaaa caaagacaat ctgagtagta ccttcaaaca gatgcgcact aaagaggaga
     8341 aataagatta cagagtttgc caacgtaccg atcaggatcc agggtttgaa cttaccccat
     8401 cgcgatcgcg tagcatttac aatccatccc ataatcggat cgttaatagc atcccagatc
     8461 ctcgccacca gaaacaaagt accgaccaaa cccacagaca gcccgacgac atcggtgtag
     8521 taatacatga ggtacatata cacaatgccg atcgcaaaat ccttcccgaa cgctccaaat
     8581 ccataactga gttttgtagt cattgaaatg ctcatagggt atcgggtcgc tgttaccaac
     8641 gcccgccttc tgttattggt atcgtcagag gtgtgcgccg gaataaatcc cccggcgcag
     8701 tagcgtttag tcgcgtttta acggtgcaac cagcttggca gccagtcgcc gtgggcggca
     8761 atcaggtcgt caacaagagc atatatttcg tcaatgccca gcacggcggc agtatgcggg
     8821 tccatcatcg cggcgtggta aacacggtcg cgattttccg taagaatagc ttcggtcagc
     8881 agcgtctgta cgttgatgtt ggtttgcatc agggcggcca gatgcgaagg tagcgtaccg
     8941 actttggtcg gctgaatgcc attagcatca accagacagg ctacttccac gcaacatcct
     9001 tgtggcaggt tatcaatcaa accatcgtta cggacgttgc cgtaaatcac actcggctcg
     9061 ccagtccaga tagcgttcat gattgtgctg gcatattccc gtgacggttt aatatcaatc
     9121 cgggaggcgt ttttatactc ctccagctct ttatgccagt tcgccagctg ctcgacgcag
     9181 cgtttcgggt actcatccag cggtacttta taacgctcaa tcaaatcctc acgacctagc
     9241 ttaataaacc acggcgtata ttcggcaaaa tgttctgatg attccgtgac gaagtagccc
     9301 agctttttga acatttcgta gcgcacaata ttctggcagc gagtattacc atgaatattt
     9361 ggcttcggtg cctgccctgc ttcataagcc gccagcagtt ccgggtagag attcacgtaa
     9421 ctgccgtcgg cggttttgcg ctccagctcc aggtaaaacg ccatatggtt gatacctgcg
     9481 caacggtaac gcagcgtagc cgggtcgata ttgaggtcac gcgccagctc ttccgccgtt
     9541 ccctgcaccg aatggcacag cccgacctgt ttgatatacg gatagcgggc atacatcgcc
     9601 caggtattca tcgccattgg gttaacatag ttgagcatgg tggcatcggg gcagacttcc
     9661 gtcatgttct cgcaaatttg ccacagatgc ggaatggtac gtagcgcgcg cataataccg
     9721 cccggcccca acgtatcggc aatggtttgt tccagaccat gccgcttaca gacctcgaaa
     9781 tcagtcaccg tgcaaggttc ataaccgcca atctgaaatg ccaccacgac aaaatcggca
     9841 tcctctaagg cttctttctg ttgggtgtgg caggtgattt tgccgctggc ccctgctgaa
     9901 tccatcagct tacgcaccac aatatgcgac tcttccaggc gggtggggtc aatgtccatc
     9961 agggcaatat gcgccgtttt cagcgcctcg cgatggaaca catcaccaag aatattttta
    10021 acgaaaatcg tcgaaccagc gccgataaat gtaattttgg gtgcagacat catgcagatc
    10081 tcctggcttg cttgaataac ttcatcatgg caggcgaatc tgcagaaaac ctccggcttc
    10141 caggttgagc attcccataa actcagatcc tgcgtgaagc agcagtaaat ctgagtttat
    10201 gggaataaat tacctgaaaa gtagagtttt cggcctcatg atgctcggac agtcccctcg
    10261 cccctctggg aagagggtga aaaccgtgct cccactcgca gtcatcctcc ctcactcctg
    10321 ccataattct gatattctag gaaagagagc catccatgaa tacagatacg tttatgtgca
    10381 gcagcgacga aaaacagacc cgcagcccgc tgtcgctgta ttcggaatac cagcggatgg
    10441 agattgagtt tcgcgcaccg catatcatgc ccaccagcca ctggcatggt caggtcgaag
    10501 tgaatgtgcc tttcgatggc gatgtggaat acctgatcaa caatgaaaaa gtgaatatca
    10561 atcagggtca tatcacgctg ttctgggcct gtacaccgca ccaactaaca gataccggaa
    10621 cctgtcagag catggcgatt tttaatctgc cgatgcatct gtttctctcc tggccgctgg
    10681 ataaagacct gattaaccac gtcactcacg gcatggtgat caaatcactt gccacacagc
    10741 aacttagccc gtttgaagta cgccgctggc agcaggaatt aaacagtccg aatgagcaaa
    10801 ttcgccagct cgccattgat gaaattggcc tgatgctcaa gcgatttagc ctctctagct
    10861 gggaaccgat tctggtcaat aaaacctcgc gcacacacaa aaacagcgtc tcgcgccatg
    10921 cgcaatttta tgttagccag atgctgggct ttattgccga aaactatgat caggcgctga
    10981 ccatcaacga tgtggctgag cacgtcaaac ttaacgccaa ctatgcaatg gggatattcc
    11041 agcgggtcat gcaattgacg atgaaacagt acattaccgc gatgcgcatc aaccacgttc
    11101 gcgcgttact gagcgatacc gataaaagta ttctcgatat tgccctgacg gcaggctttc
    11161 gttcaagtag tcgtttttac agcacgttcg gcaaatatgt cggcatgtcg ccgcaacaat
    11221 accgcaaact tagccaacag cgccgccaga cgtttcccgg ctaaagacga tatcagtatc
    11281 agccaaaaaa atagtttagc cgtcgataat aagcagt
//
LOCUS       DAZSIO010000128        11181 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZSIO010000128 DAZSIO010000000
VERSION     DAZSIO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11181
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.128"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            21..1162
                     /gene="yghO"
                     /locus_tag="ACHX5T_003065"
                     /pseudo
     CDS             21..1162
                     /gene="yghO"
                     /locus_tag="ACHX5T_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
     gene            complement(1286..2056)
                     /gene="glcC"
                     /locus_tag="ACHX5T_003066"
     CDS             complement(1286..2056)
                     /gene="glcC"
                     /locus_tag="ACHX5T_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFR8468154.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            2307..3806
                     /gene="glcD"
                     /locus_tag="ACHX5T_003067"
                     /pseudo
     CDS             2307..3806
                     /gene="glcD"
                     /locus_tag="ACHX5T_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            3806..4858
                     /gene="glcE"
                     /locus_tag="ACHX5T_003068"
     CDS             3806..4858
                     /gene="glcE"
                     /locus_tag="ACHX5T_003068"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFR8468155.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4869..6088
                     /gene="glcF"
                     /locus_tag="ACHX5T_003069"
                     /pseudo
     CDS             4869..6088
                     /gene="glcF"
                     /locus_tag="ACHX5T_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            6093..6497
                     /locus_tag="ACHX5T_003070"
     CDS             6093..6497
                     /locus_tag="ACHX5T_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFR8468156.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            6519..8690
                     /gene="glcB"
                     /locus_tag="ACHX5T_003071"
     CDS             6519..8690
                     /gene="glcB"
                     /locus_tag="ACHX5T_003071"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFR8468157.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFFRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9045..10631
                     /locus_tag="ACHX5T_003072"
     CDS             9045..10631
                     /locus_tag="ACHX5T_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HFR8468158.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
BASE COUNT         2636 a         2763 c         3108 g         2674 t
ORIGIN      
        1 tatttgttat ccacagagat atggaatgcg atctgctgat gattaaaatt gaaaaagtaa
       61 ttaataaaaa tgaccttaag gcatttatcg cttttccgtc atcactttat cccgacgatc
      121 caaactggat acccccttta ttcattgaac gcaacgagca tttgtctgcg aaaaatccgg
      181 ggacggacca tatcatctgg caggcgtggg tggcaaaaaa agcggggcag atagttgggc
      241 gtattaccgc gcaaatagat accttgcacc gtgagcgtta cggtgaagat accggtcatt
      301 tcggcatgat tgacgccatt gatgatccgc aggattttgc cgcgttgttt ggcgcagcgg
      361 aagcgtggtt gaagtcacaa ggtgcaagta agatcagcgg gcctttcagc ctgaatatca
      421 atcaggaaag cggattactg attgaaggtt ttgacacacc accctgtgcg atgatgccac
      481 acggcaaacc gtggtatgcc gcgcatattg aacaactggg ttatcacaaa ggcattgatt
      541 tactggcgtg gtggatgcag cgaaccgatc tcactttctc tccggcgcta aaaaaactga
      601 tggatcaggt gcgcaaaaag gtgaccattc gctgcatcaa tcgtcagcgg tttgccgaag
      661 agatgcagat cctgcgtgag attttcaact ctggctggca gcacaactgg ggatttgtgc
      721 cgtttaccga acatgaattc gcgaccatgg gcgaccaact taaatatctg gtgccggacg
      781 atatgatcta catcgctgag attgattctg caccctgcgc gtttattgtg gcttgcctaa
      841 catcaacgag gcgattgccg atctgaacgg atcgctcttt cccttcggct gggcaaaatt
      901 gctgtggcgt ttgaaagtca gcggtgtgcg aactgcgcga gtaccgctga tgggcgtgcg
      961 cgacgagtat cagttcagcc gcatcggccc ggtgattgcg ctgttattga ttgaagcctt
     1021 acgcgatccg tttgcccgcc ggaagatcga tgcgctggag atgtcatgga tcctcgaaac
     1081 caatacaggc atgaataaca tgctggaacg cattggggcg gagccgtaca agcgttaccg
     1141 attgtatgaa aagcaaattt aataccagat cctgtttagc gcaggggcgg tcattcggga
     1201 gagggtatag atgaacagat ttaacaccag atcctgttaa gtgcaggggc ggtcccctcg
     1261 ccttggggat agggtaaaac tctgctcaca ttcccacctt gttcatctcc agcggcaccg
     1321 ccgagcgaat caaatggtgg ccttccagct cgatatcgtg gagattcttt ttcacggtcc
     1381 gtacatgatc gcgcgctgcg cgctgagcga cgtgcggcag ccgctgcaac accgcgttgt
     1441 agatccgcgc atgctggcga tcgatctgct ttttttgctg tggtcgatgg tagagattat
     1501 ttacgctggc aaacactgaa ttaaacatca gatcggtcaa tgattgcagc gtaaacacca
     1561 gcacctgatt gtgagaagcc tgacaaatgg cgagatggaa agcgtgatcc aactgcgcat
     1621 gttcgatcag cgaaatctct ttgtggttct cactggcggc gagcattttt tcataacagc
     1681 gggttatcac aacaaaatca gcctgcgttc ccagcgttgc cgccagcctt gccgattcgc
     1741 cctccagtaa tgcgcgaacg tcgagcagat cgtacagcgt tcgcggctgc gtactgaaca
     1801 gatggatcag cgggctggtg tcctgctccc gattaagccg tgcgacatga gaatcgcgac
     1861 cctgcgccgt ttcaataatc ccgcgcccgc gcagcacggt cagcccttca cgcagtgcgg
     1921 agcgtgagaa gccgagcttt tcacacagtc gacgttccga gggaagcggc tgaccgacct
     1981 tcagtacgcc gtcgataatt aaccgttcga tactctctgc aaccacttcg caaatagggc
     2041 gacgttcatc tttcattccc ggacctcgtg cacaggtagg accaattttt ctgttcttaa
     2101 ctcgcaaaac acgcacatca cgcaagtgat tatgttacat caatttaaca ttaagttaac
     2161 caagacaagg tcacagagct ggaaaaaaaa tggtctgacc ggtagctaaa gagatagacg
     2221 aaaacgaaaa gcccgcttaa taactgttca cagaagcagc gcgcaaaaat cagctgccac
     2281 acaacacaac aaagcgaagc ctactcatga gcatcttgta cgaagagcgt cttgatggcg
     2341 ctttacccga tgtcgaccgc acatcggtac tgatggcact gcgtgagcat gtccctggac
     2401 ttgagatcct gcataccgat gaggagatca ttccttacga gtgtgacggg ttgagcgcgt
     2461 atcgcacgcg tccattactg gttgttctgc ctaagcaaat ggaacaggtg acagcgattc
     2521 tggctgtctg ccatcgcctg cgtgtaccgg tggtgacccg tggtgcaggc accgggcttt
     2581 ctggtggcgc gctgccgctg gaaaaaggtg tgttgttggt gatggcgcgc tttaaagaga
     2641 gcctcgacat taaccccgtt ggtcgccgcg cgcgcgtgca gccgggcgta tgtaacctgg
     2701 cgatctccta ggccgttgcg ccgcataatc tctattacgc gccggatcct tcctcacaaa
     2761 tcgcctgttc aattggcggc aatgtggcgg aaaatgccgg tggcgtccac tgcctgaaat
     2821 atggtctgac cgtacataac ctgctgaaaa ttgaagtgca aacgctggac ggcgaggcgc
     2881 tgacgctggg atcggacgcg ctggattcac ctggttttga cctgctggcg ctgttcaccg
     2941 gatcggaagg tatgctcggc gtgaccaccg aagtgacggt aaaactgctg ccgaagccgc
     3001 ccgtggcgcg ggttctgtta gccagctttg actcggtaga aaaagccgga cttgcggttg
     3061 gtgacatcat cgccaatggc attatccccg gcgggctgga gatgatggat aacctgtcga
     3121 tccgcgcggc ggaagatttt attcatgccg gttatcccgt cgacgccgaa gcgattttgt
     3181 tatgcgaact ggacggcgtg gagtctgacg tacaggaaga ctgcgagcgg gttaactaca
     3241 tcttgttgaa agcgggcgcg actgacgtcc gtttggcaca ggacgaagca gagcgcgtac
     3301 gtttctgggc cggtcgcaaa aatgcgttcc cggcggtagg acgtatctcc ccggattact
     3361 actgcatgga tggcaccatc ccgcgtcgcg ccctgcctgg cgtactggaa ggcattgccc
     3421 gtttatcgca gcaatatgat ttacgtgttg ccaacgtctt tcatgccgga gatggcaaca
     3481 tgcacccgtt aatccttttc gatgccaacg aacctggtga atttgcccgc gcggaagagc
     3541 tgggcgggaa gatcctcgaa ctctgcgttg aagttggcgg cagcatcagt ggggaacatg
     3601 gcatcgggcg agaaaaaatc aatcaaatgt gcgcccagtt caacagcgat gaaatcacga
     3661 ccttccatgc ggtcaaggcg gcgtttgacc ccgatggttt gctgaaccct gggaaaaaca
     3721 ttcccacgct acaccgctgt gctgaatttg gtgccatgca tgtgcatcac ggtcatttac
     3781 cttttcctga actggagcgc ttttgatgct acgcgagtgt gattacagtc aggcgctgct
     3841 ggagcaggtg aatcaggcaa ttagcgataa aacgccgctg gtgattcagg gcagcaatag
     3901 caaagccttt ttaggtcgcc cagtcaccgg gcaaacgctg gatgtgcgtt gtcatcgcgg
     3961 cattgttaat tacgacccga ccgagctggt gataaccgct cgtgccggaa cgccgctggt
     4021 ggcgattgaa gcggcgctgg agagcgccgg gcaaatgctt ccctgcgagc cgccgcatta
     4081 tggtgaagaa gccacctggg gcgggatggt cgcctgcggg ctggcggggc cgcgtcgccc
     4141 gtggagcggt tcggtccgcg attttgtcct cggcacgcgc atcattaccg gcactggaaa
     4201 acatctgcgt tttggtggcg aagtgatgaa aaacgttgcc ggatacgatc tctcacggtt
     4261 aatggccgga agctacggtt gtcttggcgt gctcactgaa atctcaatga aagtgttacc
     4321 gcgaccgcgt gcctctctga gcctgcgtcg ggaaatcagc ctgcaagaag ccatgagtga
     4381 aatcgccgag tggcaactcc agccattacc cattagtggc ttatgttact tcgacaatgc
     4441 gttgtggatc cgccttgagg gcggcgaagg atcggtaaaa gcagcgcgtg aactgctggg
     4501 tggcgaagag gttgtgggtc agttctggca gcaattgcgt gaacaacaac tgccgttctt
     4561 ctcgttacca ggtaccttat ggcgcatttc attacccagc gatgcgccga tgatggattt
     4621 acccggcgag caactgatcg actggggcgg ggcgttacgc tggctgaaat cgacagccga
     4681 tgacaatcaa atccatcgca tcgcccgcaa cgctggcggt catgcgaccc gctttagtgc
     4741 cggagatggt ggctttgccc cgctatcggc tcctttattc cgctatcacc agcagcttaa
     4801 acagcagctc gacccttgcg gcgtgtttaa ccccggtcgc atgtacgcgg aactttgagg
     4861 agcaggctat gcaaacccga ttaaccgaag agatgcggca gaacgcgcgc tggaagccga
     4921 cagcatcctg tgcgcctgtg ttcactgcgg attttgtacc gcaacctgcc caacctatca
     4981 gcttctgggc gatgaactgg acgggccgcg cgggcgcatc tatctgatta aacaggtgct
     5041 ggaaggcaac gaagtcacgc ttaaaacaca ggagcatctc gatcgctgcc tcacttgccg
     5101 caattgtgaa accacctgtc cttctggtgt gcgctatcac aatttgctgg atatcgggcg
     5161 tgatattgtc gagcagaaag tgaaacgccc actgccggag cgaatgctgc gcgaaggatt
     5221 gcgccaggta gtgccgcgtc cggcggtctt ccgtgcgctg acgcaggtag ggctggtgct
     5281 gcgaccgttt ttaccggaac aggtcagagc aaaactgcct gctgaaacgg tgaaagctaa
     5341 accgcgtccg ccgctgcgcc ataagcgtcg ggttttaatg ctggaaggct gcgcccagcc
     5401 tacgctttcg cccaacacca acgcggcaac tgcgcgagtg ctggatcgtc tggggatcag
     5461 cgtcatgtca gctaacgaag caggctgttg tggcgcggtg gactatcatc ttaatgcgca
     5521 ggagaaaggg ttggcacggg cgcgcaataa tattgatgcc tggtggcccg cgattgaagc
     5581 aggtgccgag gcaattttgc aaaccgccag cggttgcggc gcgttcgtca aagagtatgg
     5641 gcagatgctg aaaaacgatg cgttatatgc cgataaagcg cgtcaggtca gtgaactggc
     5701 ggtcgattta gtcgaacttc tgcgcgagga accgctggaa aaactggcaa ttcgcggcga
     5761 taaaaaactg gccttccact gcccgtgtac cctacaacat gcgcaaaagc tgaacggcga
     5821 agtggaaaaa gtgttacttc gtcttggatt taccttaacg gacgttcccg acagccatct
     5881 gtgctgcggt tcagcgggaa catatgcgtt aacgcatccc gatctggcac gccagctgcg
     5941 ggataacaaa atgaatgcgc tggaaagcgg caaaccggaa atgatcgtca ccgccaacat
     6001 tggttgccag acgcatctgg cgagcgccgg tcgtacctct gtgcgtcact ggattgaaat
     6061 tgtagaacaa gcccttgaaa aggaataaaa aaatgaaaac taaagtcatt cttagccagc
     6121 aaatggcgag tgcaattatt gccgcaggtc aggaagaggc gcagaaaaat aactggtctg
     6181 tttccattgc tgttgccgat gacggcggtc atctgctggc gttaagtcgc atggacgatt
     6241 gcgcgccgat tgcggcttac atctcccagg agaaagcgcg taccgccgcg ctggggcgtc
     6301 gtgaaactaa gggctatgaa gagatggtga acaacggacg taccgcgttc gtgactgcgc
     6361 cgttactgac gtcgctggaa ggcggcgtac cggttgttgt tgatgggcaa attattggtg
     6421 ccgtgggcgt ttctggttta accggagcac aggatgcgca ggtcgcgaaa gtggcagcag
     6481 cggtgttggc gaaataagcg aaaacgagga gagaaacaat gagtcaaacc ataacccagg
     6541 gccgtttacg cattgacgcc aattttaaac gttttgtgga tggagaagtt ttgccgggcg
     6601 tggaactgga cgctgctgca ttctggcaca atgttgatga gatcgttcac gatctggcac
     6661 cagaaaatcg tcagttgctg gcagaacgcg atcgcattca ggcggcgctt gatgagtggc
     6721 atcgcagcaa tccggggccg gtaaaagata aagcggccta taaatctttc tttcgtgaac
     6781 tgggctacct ggtgccgcaa ccggagcgcg tgacggtgga aaccacgggc attgacagcg
     6841 aaatcaccag tcaggcgggg ccacagctgg tggttccggc aatgaacgcc cgctacgcgc
     6901 tgaacgcggc gaacgctcgc tggggctcac tgtacgatgc gttatacggc agcgacatca
     6961 tcccgcagga aggggcgatg gtcagtggct acgatccgca gcgcggtgag caggttatcg
     7021 cctgggttcg acgtttcctc gatgaatcac taccgctgga aaacggcagc tatcaggatg
     7081 tggtggcgtt taaggtggtc gataaacaat tacgcatcca gttgaaaaat ggtaaagaaa
     7141 ccacgttacg tactccagca cagtttgtcg gttaccgtgg cgatgccgct gcgccgacct
     7201 gcattttgct gaaaaataac ggcctgcata ttgaactgca aattgatgcc aacgggcgga
     7261 ttggcaaaga cgatccggcg cacatcaacg atgttatcgt cgaagctgct atcagtacca
     7321 ttctcgactg cgaagattcg gtcgcggcgg ttgatgcgga agataaaatc ctgctgtacc
     7381 gcaatctgct gggcctgatg caggggactc tgcaagagaa aatggagaaa aacggtcggc
     7441 aaatcgtacg taaactgaat gacgatcgtc agtacaccgc cgccgatggc tctgaaattt
     7501 ctctgcacgg acgctcgttg ctgtttatcc gcaacgtggg tcatttgatg accattcctg
     7561 tgatttggga cagcgaaggc aatgaaatcc cggaaggtat tcttgatggc gtcatgactg
     7621 gcgcgattgc cctctatgat ttaaaagtgc agaaaaactc gcgcactggc agcgtctata
     7681 ttgtgaaacc gaaaatgcac ggtccgcagg aagtggcgtt cgccaacaaa ctgtttaccc
     7741 gcattgagac aatgctcggt atggcaccga ataccctgaa aatgggcatt atggatgaag
     7801 aacgccggac ctcgctgaac ttgcgtagct gtatcgctca ggcgcgcaac cgcgtggcgt
     7861 tcatcaatac cggtttcctc gaccgtaccg gcgatgaaat gcattcggtg atggaagctg
     7921 gcccgatgct gcgtaaaaac cagatgaaat cgacgccgtg gatcaaagcc tacgaacgta
     7981 ataacgtgct ttccggtctg ttctgtgggc tgcgcggtaa agcgcaaatc ggtaaaggca
     8041 tgtgggcaat gccggacctg atggcagaca tgtacagcca gaagggcgac caactgcgtg
     8101 ccggggcaaa cacagcctgg gttccgtcac caaccgctgc tacgctccat gcgctgcact
     8161 accaccatac caacgtacag agcgtgcaag ccaacattgc ccagaccgag ttcagtgctg
     8221 aatttgaacc gctgctggac gatctgctga ctattccggt ggctgaaaac gctaactggt
     8281 cggcgcaaga gatccaacaa gagctggata acaacgtgca ggggattctg gggtacgtgg
     8341 tgcgttgggt ggagcagggg attggttgtt caaaagtgcc ggatattcac aacgtggcgc
     8401 tgatggaaga ccgcgcaacg ctgcgtatct ccagccagca tatcgccaac tggttacgtc
     8461 acggtattct gaccaaagaa caggtgcagg cgtcgctgga gaatatggcg aaagtggttg
     8521 atcagcaaaa tgctggcgat ccggcttatc gtccgatggc ggggaatttc gctaactcgt
     8581 gtgcttttaa agctgccagc gatttaatct tcctcggcgt gaaacagcca aacggttata
     8641 ccgaaccgtt attacacgcc tggcgtttac gcgaaaaaga aagtcattaa tagcgtcaaa
     8701 aaagccccgg caagttttat tgtcggggct atgatagtaa ctgcggtagt acatttatcc
     8761 cggaaataac cggagcgatt atcagggttt aacaatatta aatacccgta gcgcattact
     8821 agcgggccta aaacaacaat atggacatct taacgatact ctgcgcctgc gtgtgcggaa
     8881 cagaaaccct tacccgatat acttgttatg ccagaggtag caattgcgtt agcggcgtac
     8941 tgttgctgta aagtataacg cctatatttt acagttcagt cctcttttgc ttattacaac
     9001 cttaagtata tcaagcatat aaagataata agagactgaa caatatggtt acctggaccc
     9061 aaatgtatat gccgatggga ggactggggc tatccgctct ggtcgccctg atcccgataa
     9121 tattcttctt cgttgcactc gcggtattac gtctgaaagg acatgttgct ggagcaataa
     9181 cccttatatt atctatcctg attgcgatat tcgcctttaa aatgccgatt gatatggcat
     9241 ttgctgctgc gggctatggc tttatttatg gattatggcc aattgcgtgg attattgtcg
     9301 cggcggtgtt cctgtataaa ttaaccgttg ccagcgggca gttcgatatt atccgcagct
     9361 cggttatctc catcaccgac gatcagcgtt tgcaggtgtt actgattggt ttctcctttg
     9421 gtgcgttgct ggaaggagct gctggctttg gtgcgacggt ggcgtttggt gcgttgggcg
     9481 tgccgattct ggtcgccggt caggtaacgg gaatcgatcc gttccacatt ggcgcaatgg
     9541 cgggacgtca gttaccgttc ctgtcggttc ttgtgccgtt ctggctggta gcaatgatgg
     9601 acggctggaa aggggtgaaa gagacgtggc cagcggcgct ggttgctggg ggaagcttcg
     9661 ctgtcactca gttctttacc tctaactata ttggtccgga actgccggat attacttcgg
     9721 cgctggtgag tatcgtctca ctcgctttat tccttaaagt ctggcggccg aaaaataccg
     9781 aaacggcaat cagcatgggg caatccgcag gtgcgatggt ggtaaataag ccatcttctg
     9841 gcggtcccgt gccttcagaa tatagtctgg ggcaaatcat tcgtgcgtgg tcaccgtttt
     9901 taatcttaac ggtgctggtc accatctgga ccatgaagcc gtttaaagcg ttatttgctc
     9961 cgggcggcgc gttttattca ctggtgatta atttccagat ccctcatttg catcaacaag
    10021 tgttgaaagc ggcacccatt gtcgcccaac caacgccaat ggatgcggtg tttaaattcg
    10081 accctctctc ggctggcggc accgctattt ttattgcggc gattatctct atcttcatcc
    10141 tcggcgtggg catcaagaaa gggattggcg tctttgccga aatgctaatt agtctgaagt
    10201 ggccgatatt gtcgattggc atggtgctgg cattcgcctt cgtcaccaac tattccggca
    10261 tgtccaccac gctggcgctg gtactggcag gtacaggcgt gatgttcccg tttttctcac
    10321 cgtttctcgg ctggctgggc gtattcctta ccgggtcgga cacttcttca aacgccctgt
    10381 ttggttcact gcaatcgacc acggcgcagc aaatcaacgt ctctgacact ctgctggtgg
    10441 cagcaaatac cagcggcggc gtaactggca agatgatctc cccgcaatct atcgccgtgg
    10501 cctgcgccgc gacgggcatg gtgggccgag aatctgaact gttccgctac accgtgaagc
    10561 acagtctgat ttttgccagc gttatcaccc tgctgcaggc gtatgtgttt accgggatgt
    10621 tagtctcgta aatcaataaa gccggatgaa ggtaaatatc acccggcttt ccatttgtct
    10681 gtcgtttatc tcgccaggga accgttttta cgattggctt ctttatacgt agtctccgtt
    10741 ttacgatgcg aattgtttaa tatttataca acttggcttc tgtgctggtt ataaagtacc
    10801 cactttcatg agatcaattt tagtatgtta ttacattgct tcatctgttg ctttcatgac
    10861 gaatggcgtt ttttctctta tttatatttt tcactagaac aatgagtaga tcaaaattcc
    10921 caaaactccc aaaaacataa gattattcgc aaagctgatt ttgcagaata atgagaactt
    10981 ttaagatttg tcgaggatca attttaagca atttatctcc acttgttaca taaaagatgc
    11041 cgttaggtta ataagttaat cttgctataa cagtgttcgt tactgcctat taataaagat
    11101 atttgggata gggtaacgtg gatgaggatt cgaaatcatc cgtcttgcgg aaagtgaaat
    11161 gttggcagat gttaatatta t
//
LOCUS       DAZSIO010000129        10993 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZSIO010000129 DAZSIO010000000
VERSION     DAZSIO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.129"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            92..853
                     /gene="adiY"
                     /locus_tag="ACHX5T_003073"
     CDS             92..853
                     /gene="adiY"
                     /locus_tag="ACHX5T_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR8468159.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            990..2327
                     /gene="adiC"
                     /locus_tag="ACHX5T_003074"
     CDS             990..2327
                     /gene="adiC"
                     /locus_tag="ACHX5T_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR8468160.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2431..4074
                     /gene="eptA"
                     /locus_tag="ACHX5T_003075"
     CDS             2431..4074
                     /gene="eptA"
                     /locus_tag="ACHX5T_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR8468161.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            4071..4739
                     /gene="basR"
                     /locus_tag="ACHX5T_003076"
     CDS             4071..4739
                     /gene="basR"
                     /locus_tag="ACHX5T_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR8468162.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4740..5840
                     /gene="pmrB"
                     /locus_tag="ACHX5T_003077"
     CDS             4740..5840
                     /gene="pmrB"
                     /locus_tag="ACHX5T_003077"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR8468163.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5816..5905)
                     /gene="pmrR"
                     /locus_tag="ACHX5T_003078"
     CDS             complement(5816..5905)
                     /gene="pmrR"
                     /locus_tag="ACHX5T_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR8468164.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6017..7519)
                     /gene="proP"
                     /locus_tag="ACHX5T_003079"
     CDS             complement(6017..7519)
                     /gene="proP"
                     /locus_tag="ACHX5T_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR8468165.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7783..8661)
                     /locus_tag="ACHX5T_003080"
     CDS             complement(7783..8661)
                     /locus_tag="ACHX5T_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFR8468166.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(8658..10886)
                     /gene="crfC"
                     /locus_tag="ACHX5T_003081"
     CDS             complement(8658..10886)
                     /gene="crfC"
                     /locus_tag="ACHX5T_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR8468167.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2751 a         2868 c         2692 g         2682 t
ORIGIN      
        1 tttatattca tcatgcaatt atataaccat ttcccggtat cgctttgctt tagcgagaac
       61 tggtctttta ttcgcactca ggagtacatg tatgaggatt tgcagcgacc aaccttgtat
      121 tgttttattg actgaaaaag atgtctggat aagggtgaat gggaaagaac ctattagcct
      181 taaagctaac catatggcgt tattaaattg tgaaaataat attatcgacg tctcctctct
      241 taacaacact ttggttgctc atattagtca cgacatcatc aaagattacc tccggtttct
      301 gaataaagat ctctcgcaaa taccagtatg gcaacgtagc gctacgccca tactcaccct
      361 gccatgcctg acgccagacg tctttcgcgt tgccgcgcaa cacagcatga tgcccgcaga
      421 aactgagtca gaaaaggaac gaacacgcgc attattattc acggtgctat cccgttttct
      481 cgacagtaaa aaattccttt cattaatgat gtatatgtta cgtaattgtg taagtgacag
      541 cgtttatcaa attattgaaa gcgatattca caaagactgg aatcttagta tggtagccag
      601 ttgtttatgt cttagcccaa gtctgttaaa gaaaaagttg aaaagcgaaa acaccagtta
      661 tagccaaata atcaccacct gccgcatgcg ttatgccgta aatgaattaa tgatggacgg
      721 taaaaatatc tctcaggtat cacagtcctg cggctacaac agtacgtcgt actttatttc
      781 tgtctttaaa gacttctacg gtatgacgcc gctgcattat gttagtcagc acagagaacg
      841 cactgtcgcc tgatttttaa ccttaacgaa gagctatatt aataacggca tcagcgataa
      901 cccggtcgat aataattcaa ctatcgaatg caggcgtatg atatgacgta atttattgtc
      961 acgaagctcg ccttcgcagg agtttaatta tgtcttcgga tgctgatgct cacaaagtgg
     1021 gcttaatccc cgtcaccctg atggtgtcgg ggaatattat ggggtcaggt gtttttctgt
     1081 tacctgcaaa cctggcctcc actggcggga ttgccattta tggatggttg gtgacgatta
     1141 tcggtgcgct ggggctctcg atggtatacg ccaaaatgtc gttcctcgac ccaagtcctg
     1201 gtggttctta cgcttacgcc cgccgctgct ttggcccgtt tctcggttat caaaccaacg
     1261 tcctctactg gctggcctgc tggatcggca atatcgccat ggtggtcatt ggcgtaggat
     1321 atttaagtta cttcttcccg attctgaaag atccattggt attaaccatc acctgcgtcg
     1381 tggtgctgtg gatcttcgtc ctgctgaaca ttgtcggtcc gaaaatgatc acccgcgtgc
     1441 aggccgttgc cactgtgctg gcgctgatcc ccatcgtcgg gattgccgta tttggctggt
     1501 tctggttccg tggtgaaacc tatatggcgg catggaacgt cagcggcctg ggcaccttcg
     1561 gtgcgattca aagtaccctt aacgttacgc tgtggtcgtt catcggtgtg gaaagtgcct
     1621 ccgttgccgc aggtgtggtg aaaaacccga aacgcaatgt ccctatcgcc accattggtg
     1681 gggtattgat tgccgccgtt tgctatgtgc tctccaccac cgcgattatg gggatgatcc
     1741 ctaatgccgc actgcgcgtt tctgcctcgc cattcggtga tgccgcacgg atggcgttgg
     1801 gtgacaccgc tggggccatt gtttccttct gcgcagctgc gggttgctta ggttcactgg
     1861 gcggctggac gttgctggcg ggtcaaacgg cgaaagccgc tgccgatgac ggactgttcc
     1921 caccgatttt tgcccgtgta aataaagcgg gtacgccagt ggcggggctg attatcgtcg
     1981 gtattttgat gaccatcttc cagctcagca gcatttcacc aaacgcgacc aaagagttcg
     2041 gtctggtttc ttccgtctcg gtcatcttta cactggtgcc atatctttac acctgtgcgg
     2101 cgttactgct gctcggccac ggtcactttg gtaaagcacg cccggcatat ctggctgtta
     2161 ctaccattgc cttcctctac tgcatttggg ccgtggtggg gtccggggcg aaagaggtta
     2221 tgtggtcatt tgtcaccctg atggtcatca ccgccatgta tgcgctgaat tacaaccggc
     2281 tacataaaaa cccgtatccc ttagatgcac caataagcaa agattaattc cccttaatcc
     2341 agcaaacata aaagccaacc ttaagaactt aaggttggct taattttgct ttgcgagcat
     2401 atgcgcactt tgttcgatgg aaacaccgtg atgttgaagc gcctactaaa aagaccctct
     2461 ttgaatttac tcgcctggct attgttggcc gctttttata tctctatctg cctgaatatt
     2521 gcctttttta aacaggtgtt gcaggcgctg ccgctggatt cgctgcataa cgtactggtt
     2581 ttcttgtcga tgccggttgt cgctttcagc gtgattaata ttgtcctgac actaagctct
     2641 ttcttatggc ttaatcgacc actggcctgc ctgtttattc tgattggcgc ggctgcacaa
     2701 tatttcataa tgacttacgg catcgtcatc gaccgctcga tgattgccaa tattattgat
     2761 accactccag cagaaagtta tgcgctgatg acaccgcaaa tgttattaac gctgggattc
     2821 agcggcgtgc ttgctgagct gattgcctgc tggctaaaaa tcaaacctgc cacctcgcgt
     2881 ctgcgcagtg ttcttttccg tggagccaat attctggttt ctgtactgct gattttgctg
     2941 gtcgccgcac tgttttataa agactacgcc tcgttgttcc gcaataacaa agagctggtg
     3001 aaatccttaa gcccctctaa cagcattgtt gccagctggt catggtactc ccatcagcga
     3061 ctggcaaatc tgccgctggt gcgaattggt gaagacgcgc accgcaaccc gttaatgcag
     3121 aacgaaaaac gtaaaaattt gaccatcctg attgtcggcg aaacctcgcg ggcggagaac
     3181 ttctccctca acggctaccc gcgtgaaact aacccgcggc tggcgaaaga taacgtggtc
     3241 tatttcccta ataccgcatc ttgcggcacg gcaacggcag tttcagtacc gtgcatgttc
     3301 tcggatatgc cgcgtgagca ctacaaagaa gagctggcac agcaccagga aggcgtgctg
     3361 gatatcattc agcgagcggg catcaacgtg ctgtggaatg acaacgatgg cggctgtaaa
     3421 ggtgcctgcg accgcgtgcc tcaccagaac gtcaccgcgc tgaatctgcc tgatcagtgc
     3481 atcaacggcg aatgctatga cgaagtgctg ttccacgggc tggaagagta catcaataac
     3541 ctgcagggtg atggcgtgat tgtcttacac accatcggca gccacggtcc gacctattac
     3601 aaccgctatc cgccgcagtt caggaaattt accccaacct gcgacaccaa tgagatccag
     3661 acctgtacca aagagcaact ggtgaacact tacgacaaca cgctggttta cgtcgactat
     3721 attgttgata aagcgattaa tctgcttaaa gaacatcagg ataaatttac caccagcctg
     3781 gtttatcttt ctgaccacgg tgaatcgtta ggtgaaaatg gcatctatct gcacggtctg
     3841 ccttatgcca tcgccccgga tagccaaaaa caggtgccaa tgctgctgtg gctgtcggag
     3901 gattatcaaa agcggtatca ggttgaccag aactgcctgc aaaaacaggc gcaaacgcaa
     3961 cactattcac aagacaattt attctccacg ctattgggat taactggcgt tgagacgaag
     4021 tattaccagg ccgcggatga tattctgcaa acttgcagga gagtgagtaa atgaaaattc
     4081 tgattgttga agacgatacg ctgttattgc agggactgat tctggcggcg caaaccgaag
     4141 gctacgcgtg cgatggcgtg acaaccgcgc ggatggcgga acaaagcctt gaggacggtc
     4201 attacagcct ggtggtactg gatttagggt tacccgacga agatggactg cattttctcg
     4261 cccgtatccg gcagaaaaaa tacaccctgc cggtactgat cctcaccgct cgcgatacgc
     4321 tgaccgacaa aatcgccggg ctggatgtcg gtgccgatga ctatctggtg aagccttttg
     4381 cgctggaaga gttacacgcc cgtatccgcg ccctgctacg acgccataat aatcagggcg
     4441 aaagtgagct gattgttggc aatctgacgc tgaatatggg tcgccgtcag gtatggatgg
     4501 gcggtgaaga gttgattctg acgcccaaag aatatgctct gctgtcacgg ttaatgctca
     4561 aagcaggcag tccggtacat cgggaaattc tctacaacga catctataac tgggacaatg
     4621 aaccctcgac caacaccctg gaagtgcata tccacaatct gcgcgacaaa gtgggcaaag
     4681 cccgtatccg caccgtgcgc ggctttggct atatgctggt cgcgaatgag gaaaactaat
     4741 tgaatctgat gcgttttctg cgccgaccta tatcgctgcg ccaacggctg atattgacca
     4801 tcggggccat tttgttggtg tttgagctga tcagcgtctt ctggctatgg catgaaagta
     4861 ccgagcagat tcagctgttt gagcaggcgc tacgcgacaa tcgcaacaac gatcgccaca
     4921 tcatgcgtga gatccgcgag gcggtcgcca gcctgattgt ccccggcgtc tttatggtca
     4981 gcctgacgct atttatctgc tatcaggcgg tacgccgcat cacccgcccg ctggcggagc
     5041 tgcaaaaaga gctggaagcg cgcaccgccg acaacttaac gcccattgcc attcacagcg
     5101 ccaccctcga aatcgaagcg gtggtttcgg cgttgaacga tctggtcagt cgcctgacca
     5161 gcacgctgga taacgaaagg ttgtttaccg ctgacgtcgc gcacgaactg cgaacgccac
     5221 tggcgggggt gcgtttgcat ctggaactgc tggcgaaaac gcatcacatt gatgtagcac
     5281 cgttagtggc acggcttgat cagatgatgg agagcgtctc ccagctgctg caactggcgc
     5341 gtgccggaca gtcattttct tccggtaatt atcaacatgt aaaactgctg gaagatgtca
     5401 ttctcccctc gtatgacgaa ctcagtacca tgctcgacca gcgacagcaa accctgctat
     5461 tgccagagag cgccgccgac atcaccgttc agggtgatgc aaccctgctg cggatgttat
     5521 tgcgaaacct ggtagaaaac gcccatcgtt acagcccgca aggcagcaac attatgatta
     5581 agctgcaaga agatggcgga gcggtcatgg cagttgaaga tgaaggacca ggtattgatg
     5641 agagtaaatg cggggagttg agtaaagcgt ttgtacgtat ggacagccgt tatggcggga
     5701 ttggtctggg gttaagtatt gtcagccgca ttacacagtt gcatcacggg cagtttttcc
     5761 tgcaaaaccg gcaagagact tccggcacgc gggcctgggt acggctgaag aaagatcaga
     5821 acgtggcaaa ccagatataa agaaagctgc tgaccaccag cacgctgaac acggtagtta
     5881 aactttcata aacacggttt ttcattgcac tctcctccgt taacctggag gagagtatgc
     5941 gcgtcagaga ttaagtgaac cttaagagtt caattggcct gataagacag cgtcacatca
     6001 ggccatccgt ttcagcttat tcatcaattc gcggatgttg ctgcaccagg cgggtacgtt
     6061 tcgcctgcaa atcggcaatc tcgtggtcaa tattatcgat tttctgctcg atattatcgt
     6121 aatgctcgac gagaatttcc ttcgcttcct gtatatctga cgccgccggt gtcgcacctt
     6181 tcaacggacg atttgccgtc tctttcatgg ttacgccggt gattaaacca atcaccgcca
     6241 ctaccatcag gtaataggca ggcatcatca gattctgcga gctttcgacc agccaggccg
     6301 ccagtgttgg cgtcagaccg gcaaccagca ccgaaatatt aaatgccgcc gccagcgcgc
     6361 tgtaacggat atgcgtcggg aacatcgctg gcaaggtaga agccataacg cccataaagc
     6421 agttaaggat caccgccagc atcagtaacc cggcaaaaat caggccgatg acgttactgt
     6481 taatcagaat aaacgccggg atcgccaaca caaacagtgc aacactacca agtagcacaa
     6541 acggacgacg gccaaaacgg tcactcagca agcccatcac cggctggaca aacagcatac
     6601 cgatcataat ggcgataata atcagcaccc cgtggtcttc ggagtaatgc aggttatgcg
     6661 acaaataact cggcatatag gtcagcaaca tgtagtaagt cacgttggtg gcaattacca
     6721 gaccaataca tgtcaacagg ctgcgccagt atttagtggc aatctcttta aacgagactt
     6781 tcgggccatc ctgcaaacct tcgcggtcgc cctgttccag tttatcaaca tgctgctgga
     6841 acgccggagt ttcttccagc gcatggcgca ggtaaagccc gataatccct aacggcagag
     6901 caataaagaa cggaatacgc cagccccagt cgaggaagtt cgcttcgccg acaatggtcg
     6961 aaattaacac caccacgccc gcacccagca caaacccggc aatagaaccg aagtccagcc
     7021 agctgcccat aaagccacgt ttacggtcag gggagtattc cgcaacaaat atcgacgccc
     7081 cggtatattc accaccgacc gagaaacctt gtgccatctt acagatcaac agcagaatcg
     7141 gtgcccaaat accaatcgtg tcgtaggacg gtattaagcc aatacagaac gtactgatcg
     7201 acataatcac aatagtgata gcgaggatct tctggcgacc atatttatcg cccaacatac
     7261 caaagaagag tccgccaagc ggtcgaatca gaaagggaac ggagaaagtg gcaagtgcag
     7321 caaccatctg cacgctgggg tcagcccccg ggaaaaaaac tttacctaat gcgtaagcaa
     7381 caaaaccata aacaccaaaa tcaaaccatt ccattgcatt acccagtgat gctgcggtaa
     7441 tggctttacg cagtttaccg tcatcaataa tggtgacatc acgaagggta atcggtttta
     7501 cttttttcct tttcagcata gctttcctcg cagagttggc ccatttccgc aaacgccaga
     7561 aaaatcagcg cgacggccgc aaaaggggag cgggaattgc aggatgcaat ctctgtaaca
     7621 agcgtagcgg gtttgcttac accctccggt taaggaatgt acaatcaaac cagttgacgc
     7681 ctgaattggg cagttaatga cctttttacc ctaccagtgt ttaaatttgt gatcaacttc
     7741 acacatggat ttcgtttagg actcattgat gtaactgatt atttacgcca aatttacaga
     7801 gaagatttcc tcgaccattg cccccaccaa acgctgcgct gaacagagtc gtggcatccc
     7861 cagcgcgaca gtttgccagc gttgggtcac tgaccagcaa acgggttgtt tatctgccgt
     7921 ggtccacttt cccagccaac ttaaaaaatc agcatgatcc ccgcccgtcg gaacggtcga
     7981 agaggcaaac cactgatggt aatagtgacg cgtagtcggt gccgcattga tactctctgc
     8041 cagatgttgt gtcgctttct cgcgcaaatc acccagcaac aacaccaccg cttgcgggct
     8101 tcccagacgg caggcatcgc caaatgcacc ccagcgcaac tcctccagaa cgataaaaca
     8161 gcgcccaggc aacgaccagg cgttatacgc cccagcacgc cagcgggtaa aaatttgctc
     8221 gctctgatca cctgcctgca ccagtaggcc tcgttgcgtt aacgcctttt ccttccagcg
     8281 acaacgggct gaaaaccact catacaaggc ggcaatttgc tgggggagct ccgggaaagc
     8341 agagtgctgg cggtgatcca ttttttgcag cagcgccaga tgctgcgcga tctgcgtcag
     8401 cgccagatga ccgggatcca gcatcgacgc cagtttttcc accattgtca gtcgcaggct
     8461 aagcggcgca tttaacatgc cgccgttcgt ccatgtctga agctgcacac gcgaaagaag
     8521 ttcctgcatc agtcgttcac gaaactgctg ctgatgggct tgaggatggc gggcatcatc
     8581 gccctgcgcc agatccacta aaaagcgcgg atgaaccgac tccagcacgc gaccggggcc
     8641 gtccagtaac gtcttggtca atatcgttct gccgtgaaaa gtgtttgaat atcatcgcgt
     8701 aacagctgag cgtcggtgta aatccagctg tgagtcatca cagtctgctg caattgctgc
     8761 tgcatcagcc tgaccaccga ttcattttgt tgacgcagag ccagactttc gcgtaaacgc
     8821 gtctgtaatt ctgtcaaaca tgaagcgaac tcagcgaaaa aagtattcat gcctgccgta
     8881 acagattcat cgacctgctc tgccagaact ttagccattt gttggcaata gagatcgact
     8941 tctgcgctta atgctcgttg caacacactg taatcaaccg tttctgtcgg ggatttctca
     9001 tttccccgtc cccagtcggg ctgattcaac cagcgcgaaa aagtctcacg cacaacgcct
     9061 aaacgcgtgc tctgctcgtc cgttgcttcc tggcgcgaaa tgacttcact gaacagctgg
     9121 cgagtgttga agtggggaac tacgccgtga aaaacaggaa aatgaaaccc aggacgaaac
     9181 cctgactcgc tcaattccat tttgacttgt tgctcaatgg ggcgaataac atcggttaac
     9241 acccggcaaa gggtggattc catctcggca aaacgcagcg taaagtcgcg actgatggtg
     9301 ttctgcgccg tctgtaacag tgtctcacag cgggtacgaa tctcgcttaa cggctccgaa
     9361 tcatcctgaa acaaggcggc taactgcgca ttcaccgcat cttgctgttg acgcagaaag
     9421 tggttggcgg aggtcagggc cagctcgatt tcatgtttaa tctcgccgct cacctgcgcc
     9481 tgattgagtt gcaatagctg caaactttct tcgacctgat ggatattttg ccgcaattgt
     9541 tcacaagcga cgtttaaccc gtgcgcacga aaatccaggt attcccgcgc ctgctgcgcg
     9601 taattcaaca gtttatgcgc agcagatcgc agagcataca atgaggcgtt agcgtaagcg
     9661 gcatgaagca acgcctgaat tggctgggcg aacagcgaat cttcccacaa ctgatcggca
     9721 gcatgacgaa tatgttcgag gtccgccaga tcggcatgac gccagcgcct gccgagcgcg
     9781 gcatgggcaa aatcttccac ccagcgttgt tgctctggcg ctggtaactt accgttgttg
     9841 gctaactcat agcgcgcccg attcgccagg tagccccaca tcgacgacac cgggaatatc
     9901 tgctgtggcg taatacagcc tttcatcagc gtcccggaaa tcagtgcccg cacctggtcg
     9961 gcgtcgtcac tgttacgatc ctgttgatcg aacttattga ccagcacata cagcggcacc
    10021 gattgcccca ccgccaaaat cgcctcacgg acctcttcat cggagatcga tttcagttgc
    10081 gtataatcca gcaccgccag taccgccgag gcgcgtgcca gctgctggtt aagcattttt
    10141 tgcagatgcg gttgcccggc ttcatttggc ccgggggtat ccagtaacgt caactgaccg
    10201 ggataacttt ccagccccgc cagatggaca aactccactt caatcacggg aatatgctca
    10261 atggcggcgt aagcagaaaa aggaaaatcg acgtccagcg ccttcgccag tcgcactaaa
    10321 tcattcaaac ttttcagaca atgaaaaata ggctgggcac ccagataata tttttcgaaa
    10381 gcgacgccat tttcgatccg ctgcataagc gcacgcatat ctttatctat ttccagcaca
    10441 tcggtcagat gcttaatatc gcaatcacgc aggcgctgtt gtaattgttg aattaaacaa
    10501 tcgattggcg cgacatgtga aaaatgcagt accggttcct tttgcccggg cgtatggcga
    10561 ataagcgtcg gcagcgcagt cattgggcga ttacgattag gcagaacctc cgtaccaaca
    10621 atggcattaa tggtggttga tttccctgct ttcatggtac cgacaattgc cagcaccatt
    10681 tccagtcggg aaattttaca caactcatta ttcagcatcg cgtgacgttc ggcgatatta
    10741 ggctgactcc agggtaaagc cagttgtggc gcgtcgtctc cgggtacaga gagaggcatt
    10801 ttttccagta actgcaactg ttggcgagaa agctgtaaca ggcgttcagc ctcctgactt
    10861 aactcataca gggtctgtgt gtacatagaa aattcttcct taaagcaaat tttgttactt
    10921 ttatttagcc agattgtttt tgagttctgt tttcggcttt tataattact gcaagaaata
    10981 attttatatt tag
//
LOCUS       DAZSIO010000130        10931 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZSIO010000130 DAZSIO010000000
VERSION     DAZSIO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.130"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            110..1009
                     /gene="lysO"
                     /locus_tag="ACHX5T_003082"
     CDS             110..1009
                     /gene="lysO"
                     /locus_tag="ACHX5T_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR8468168.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1153..2805
                     /gene="hcp"
                     /locus_tag="ACHX5T_003083"
                     /gene_synonym="priS"
     CDS             1153..2805
                     /gene="hcp"
                     /locus_tag="ACHX5T_003083"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR8468169.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            2817..3785
                     /gene="hcr"
                     /locus_tag="ACHX5T_003084"
     CDS             2817..3785
                     /gene="hcr"
                     /locus_tag="ACHX5T_003084"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR8468170.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            3918..5636
                     /gene="poxB"
                     /locus_tag="ACHX5T_003085"
     CDS             3918..5636
                     /gene="poxB"
                     /locus_tag="ACHX5T_003085"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR8468171.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            5673..6674
                     /gene="ltaE"
                     /locus_tag="ACHX5T_003086"
     CDS             5673..6674
                     /gene="ltaE"
                     /locus_tag="ACHX5T_003086"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR8468172.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            6685..8115
                     /locus_tag="ACHX5T_003087"
     CDS             6685..8115
                     /locus_tag="ACHX5T_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR8468173.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8214..9227
                     /locus_tag="ACHX5T_003088"
     CDS             8214..9227
                     /locus_tag="ACHX5T_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR8468174.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9224..10054)
                     /gene="amiD"
                     /locus_tag="ACHX5T_003089"
     CDS             complement(9224..10054)
                     /gene="amiD"
                     /locus_tag="ACHX5T_003089"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR8468175.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10051..10374)
                     /locus_tag="ACHX5T_003090"
     CDS             complement(10051..10374)
                     /locus_tag="ACHX5T_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR8468176.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(10585..>10931)
                     /locus_tag="ACHX5T_003091"
     CDS             complement(10585..>10931)
                     /locus_tag="ACHX5T_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR8468177.1"
                     /translation="LYGCLLSLYSICARDIYNMNAGNNVKVAADAFLEIIFSLKNKYC
                     IKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRYRPQSQDGEDFR
                     SHLQYKLPLSAH"
BASE COUNT         2501 a         2897 c         2883 g         2650 t
ORIGIN      
        1 cccctctgat gcataacatt tccttttaat tgcaacttta ctcgcccttc cgctttcagt
       61 agtgtgtcat cgcgcatttc gacttcttct ttgcaaggac tggattttca tgttttctgg
      121 gctgttaatc attctggttc ccctgattgt gggttacctc attccgcttc gccaacaagc
      181 tgcgttaaaa gctattaatc agctattaag ctggatggtt taccttattc tcttttttat
      241 gggtatcagt ctggcgtttc tcgataacct cgccagtaac ctgttggcga ttctgcatta
      301 ttctgccgtc agtattaccg ttattttact gtgtaatatt gccgccctga tgtggctgga
      361 gcgaggcctg ccgtggcgca accaccatca gcaagaaaaa ctcccgtcgc gtattgcgat
      421 ggcgctggag tcgctaaaac tgtgcggcgt agtagtgatt ggttttgcca ttggtctgag
      481 tggactggct ttcttacaac acgcgaccga agccagtgaa tacacgttaa ttttgctact
      541 tttcctcgtt ggtattcagt tgcgcaataa tggcatgacc ttaaagcaga ttgtccttaa
      601 tcgccgggga atgattgtcg ccgtggtggt ggttgtcagt tcattaattg gtggtttaat
      661 taacgccttt attcttgatc tccccatcaa taccgcgctg gcaatggcct ccggtttcgg
      721 ctggtattct ctttccggta ttttattgac cgaatctttt ggtccggtaa tcgggagcgc
      781 ggcgtttttt aatgatctgg cccgtgaact gattgctatt atgttgatcc ctgggctgat
      841 tcgccgcagc cgctctactg cactgggctt atgcggtgcc acatcaatgg atttcaccct
      901 gcccgttctt caacgtactg gcgggctgga tatggtcccg gcggcaattg ttcacggttt
      961 tattcttagc ctgttagtgc cgatcctcat cgcctttttc tctgcgtaat acctctctgg
     1021 cggtagttcc ctgccgccaa aattgcgcta aatcaatctc ccttaaagtt gcatgaaaaa
     1081 tcccttttat ccccgcgtta agcgtcttac ccttaaacat gtatattaaa tataacttta
     1141 aaaggtgtga tcatgttttg tgtgcaatgt gaacaaacta tccgtactcc ggcagaaaac
     1201 ggctgctcat acgcgcaggg gatgtgtggt aaaacggcgg aaacttctga ccttcaggat
     1261 ttactcatcg cggcgctgca agggctttct gcctgggcgg taaaagcgcg tgaatacggc
     1321 atcatcaacc acgatgttga cagcttcgcg ccacgtgcct ttttctcaac cctgaccaac
     1381 gttaacttcg attctccgcg tattgtcggc tacgctcgtg aagcgattgc cttgcgcgag
     1441 gcgctgaaag cacaatgcct ggctgtagat gccaacgcgc gcgtcgataa cccgatggct
     1501 gacctgcaac tggtgagcga tgatctcggc gagctgcaac gtcaggcagc agaatttacc
     1561 cctaacaaag ataaagcggc gattggcgaa aacattctcg gcctgcgtct gctgtgcctg
     1621 tatggcctga aaggtgcggc ggcctatatg gaacacgcgc atgttctcgg tcaatacgac
     1681 aacgatattt atgcccagta ccataaaatc atggcgtggc tggggacctg gcctgccgat
     1741 atgaacgcgc ttcttgagtg ttcaatggaa atcggccaga tgaacttcaa agtgatgagc
     1801 attctggatg caggcgaaac cggtaaatac ggtcacccga cgccaaccca ggtcaacgtc
     1861 aaagcgacgg cgggtaaatg cattctgatt tccggtcacg atctcaaaga tctctacaac
     1921 ctgctggaac agaccgaagg cacgggcgtt aatgtctaca cccacggcga aatgctgcct
     1981 gcgcatggct acccggagct gcgtaaattc aagcatctgg tcggtaacta cggcagcggc
     2041 tggcagaatc agcaagtgga gttcgctcgt ttccctggcc ccatcgtgat gacctcgaac
     2101 tgcatcatcg acccaaccgt aggcgcttat gacgatcgta tctggacccg cagcatcgtt
     2161 ggctggcctg gcgtgcgtca tctggatggc gaagatttct ctgcggtcat cgcccaggcg
     2221 caacaaatgg cgggcttccc gtacagcgaa attcctcacc tgatcaccgt gggctttggt
     2281 cgccagacgc tgcttggtgc agccgatacg ctgattgatc tggttagccg tgaaaaactg
     2341 cgtcatatct tcctgcttgg tggctgtgac ggcgcacgcg gcgagcgcca ctacttcacc
     2401 gatttcgcca ccagcgtgcc ggatgactgc ctgatcctga ccctcgcctg tggtaaatat
     2461 cgctttaaca aacttgagtt tggcgatatc gaaggtctgc cgcgtctggt agatgcaggt
     2521 caatgtaacg atgcttactc agcaattatt ctggctgtca ctctggcaga gaaactgggc
     2581 tgtggtgtga acgatctgcc gctgtcgctg gtgctctcct ggtttgaaca gaaagcgatt
     2641 gtcattctgc tgacgctgct ttctctgggc gtgaaaaata tcgtcaccgg cccgactgca
     2701 cctggtttcc tgacaccaga cctgctggcg gtgctgaacg agaaattcgg cctgcgttct
     2761 atcaccactg ttgaagaaga catgaagcaa ctgttgaacg cgtaaggagt ttgtcgatga
     2821 cgatgccaac gaatcaatgc ccgtggcgga tacaggttca tcacattacg caagaaacgc
     2881 cggatgtgtg gacgatttcc ctgatttgcc acgatcacta cccatatcgc gccgggcaat
     2941 atgcactggt cagcgtgcga aactcagcgg aaacgctgcg tgcttacacc atttcctcca
     3001 cgccaggcgt gagtgaatat atcaccctga ccgtgcggcg gattgatgac ggtgtcggct
     3061 cccagtggct gacgcgcgat gtaaaacgcg gtgattatct ctggctttcg gacgcgatgg
     3121 gggaatttac ctgcgacgat aaagcagaag ataaattcct gttgctggcg gcaggctgcg
     3181 gcgtcacgcc gattatgtcg atgcgtcgct ggcttgcgaa gaaccgtcca caggccgatg
     3241 tgcgggtgat ctacaacgtg cgtacgccgc aggatgttat tttcgccgat gagtggcgta
     3301 actatccggt aacgctggtg gcagaaaata acgttaccga aggctttatc gctggtcgcc
     3361 tcactcgcga actgctggca gatgtacctg acttagcttc acgtaccgtg atgacctgcg
     3421 gcccggctcc gtatatggat tgggtagagc aggaagtgaa agcgctcggc gtgacgcgtt
     3481 tctttaaaga gaaattcttc accccagtag cagaagcggc gaccagcggt ctgaaattca
     3541 ccaaactgca accggcacga gaattttacg ccccggttgg caccacgcta ctggaggcgc
     3601 tggaaagcaa taacgttccg gttgtcgctg cctgccgtgc gggtgtttgc ggctgctgta
     3661 agacaaaagt ggtttccggt gaatatacgg tgagcagtac aatgacgctg accgacgccg
     3721 aaatcgctga aggttacgta ctggcctgct cctgccatcc gcagggggat ttggttctcg
     3781 cataatcgcc ttatgcccga tgatattcct ttcatcgggc tatttaaccg ttagtgcctc
     3841 ctttctctcc catcccttcc ccctccgtca gatgaactaa acttgttacc gttatcacat
     3901 tcaggagatg gagaaccatg aaacaaacgg ttgcagctta tatcgccaaa acactcgaat
     3961 cggcaggcgt gaaacgcatc tggggagtca cgggcgactc tctgaacggt cttagcgaca
     4021 gtcttaatcg catgggcacc atcgagtgga tgtccacccg ccatgaagaa gtggcggcgt
     4081 ttgccgctgg cgctgaagca caactttcag gagaactggc ggtctgtgcc ggatcgtgcg
     4141 gcccaggcaa cctgcacttg atcaacggcc tattcgattg ccaccgcaat aacgttccgg
     4201 tactggcgat tgccgctcat attccctcca gcgaaattgg cagcggctat ttccaggaaa
     4261 cccacccaca agagctattc cgcgaatgta gtcactattg cgagctggtt tccagcccgg
     4321 agcagatccc acaagtgctg gcaattgcta tgcgcaaagc ggtgcttaac cgtggcgttt
     4381 ccgttgttgt attaccgggc gacgtggcgt taaaacctgc gccagaaggg gcaaccacgc
     4441 actggtatca tgcgccacag ccagtcgtga tgccggaaga agaagagtta cgcaaactgg
     4501 cgcaactgct gcgttattcc agcaatatcg ccctgatgtg tggcagcggc tgcgcggggg
     4561 cgcataaaga gttagttgag tttgccggga aaattaaagc acctattgtt catgccctac
     4621 gcggtaaaga gcatgtcgaa tacgataatc cgtatgatgt tggaatgacc gggttaatcg
     4681 gattctcgtc aggtttccat accatgatga acgccgacac gttagtgcta ctcggtactc
     4741 agttccccta ccgtgcgttc tacccgaccg atgccaaaat tattcagatt gatattaatc
     4801 cagccagcat cggcgcgcat agcaaggtag atatggcgct ggtcggcgat atcaaatcaa
     4861 ccctgcgcgc attgctgcca ctggtggaag aaaaaaccga tcgcaagttt ctggataaag
     4921 cgctggaaga ttaccgcgat gcccgcaaag ggctggacga tttagctaaa ccgagcgaga
     4981 aagccattca cccgcaatat ctggcgcagc aaattagtca ttttgccgcc gatgacgcca
     5041 tctttacctg tgacgtcggc acgccaacgg tatgggcggc acgttatctg aaaatgaacg
     5101 gcaagcgtcg cctgttaggt tcgtttaacc acggttcgat ggctaacgcc atgccgcagg
     5161 cgttgggtgc gcaggcaacc gagccggaac gtcaggtggt cgccatgtgc ggcgatggcg
     5221 gttttagcat gttgatgggc gatttcctct cagtagtgca gatgaaactg ccggtgaaaa
     5281 ttatcgtctt taataacagc gtgctgggct ttgtggcgat ggagatgaag gccggaggtt
     5341 acctgacaga cggtactgag ctgcacgaca ccaactttgc ccgcattgcc gaagcgtgcg
     5401 gcattacggg tatccgtgta gaaaaagcct ctgaagttga tgaagccctg caacgcgcct
     5461 tctctatcga cggtccggtg ttggtggatg tggtggtcgc caaagaagaa ttagccattc
     5521 caccgcagat caaactcgaa caggccaaag gttttagcct gtatatgttg cgcgcaatta
     5581 tcagcggacg cggcgatgaa gtgatcgaac tggcgaaaac gaactggcta aggtaaaaag
     5641 ggtggcattt cccgtcataa taaggacatg ccatgattga tttacgcagt gataccgtta
     5701 cccgaccgag ccgcgccatg ctcgaagcga tgatggccgc cccggttggg gacgacgttt
     5761 acggagacga ccctaccgtt aatgctctgc aggactatgc agcagagctt tccggtaaag
     5821 aagccgccat ttttctgcct accggcactc aggccaacct ggtcgctctg ctcagtcact
     5881 gcgaacgtgg cgaagagtat attgtcggtc aggccgcgca taactatctg tttgaagccg
     5941 gtggcgcagc ggtgctgggc agtattcagc cgcaacccat cgacgcggct gccgacggca
     6001 cgctaccgct ggataaagtg gcgatgaaaa tcaaacccga cgatatccat ttcgcccgca
     6061 ccaaattact cagtctggaa aacacccaca acggcaaagt gctgccgcgt gaatacctga
     6121 aagaagcatg ggaatttacc cgcgagcgca atctggcgct gcatgttgac ggtgcgcgca
     6181 tctttaatgc cgtggtggct tacggctgcg aactgaaaga gatcacgcaa tattgtgatt
     6241 cgttcaccat ttgcctgtcg aaaggtcttg ggacgccagt cggttcatta ctcgtcggta
     6301 atcgtgatta cattaaacgt gccatccgct ggcggaaaat ggccggtggc gggatgcgtc
     6361 agtccgggat tctggctgcc gccgggatgt atgccctgga aaataacgtc gcacggttgc
     6421 aggaagatca cgacaacgcc gcctggatgg cggagcaact gcgtgaagca ggcgcggatg
     6481 tgatgcgtca ggacactaat atgctgtttg ttcgcgtcgg ggaagaaaat gctgccgcgt
     6541 taggcgaata catgaaagcg agaaacgtgc tgattaacgc ctcgccgatt gtccgcctgg
     6601 tgacgcatct tgacgtctcg cgcgaacaac tggcggaagt cgccgcccac tggcgcgcat
     6661 tcctggcgcg ttaaggagag agacgtgccg caacgcattt tagttctcgg tgccagtggc
     6721 tacattggtc agcatctggt gcgcacactc agccagcaag ggcatcagat cctagcggcg
     6781 gcacgtcatg tcgacaggct tgcaaagctg caactggcaa acgtcagttg ccataaagtc
     6841 gatctcagct ggccggataa ccttccggcc ctgttgcagg atatcgatac cgtctatttt
     6901 ctggtgcaca gcatgggcga aggcggcgat tttatcgctc aggagcgcca gctggctctc
     6961 aacgtccgcg atgcgctacg tgaagtacca gttaagcaat taatctttct cagttcgttg
     7021 caggccccgc cacatgagca gtcggatcat ctgcgagctc gtcaggctac ggcggacatt
     7081 cttcgtgaag cgaatgtacc agtgaccgaa cttcgggccg gaattatcgt tggcgcaggt
     7141 tcagcggcgt tcgaagtcat gcgcgatatg gtctacaacc tgccagtgtt aacgccgcca
     7201 cgctgggtac gttcacgcac cacgcccatc gcgctggaaa acttgctgca ctatctggtg
     7261 gcgctgttag accatccagc caacgaacac cgcatcttcg aagccgccgg accagaggtg
     7321 ctcagttatc agcaacagtt tgaacatttt atggcggtga gcggtaagcg ccgctggttg
     7381 atccccatcc ccctccccac ccgctggatt tcggtgtggt ttctcaatgt gattacttcc
     7441 gtaccgccca ccaccgccag ggcgttgatt caggggctga aacacgatct gctggcagat
     7501 gataccgcgc tacgtgcact catcccacaa cggctgatcg ctttcgatga cgcggtacgt
     7561 agcacgttga aagaggaaga aaaactggtc aactccagcg actggggcta cgacgctcag
     7621 gcctttgccc gctggcgacc ggagtacggt tatttcgcca aacaggcggg gtttaccgtt
     7681 aaaacgtccg ccagccttgc ggctttatgg ctggtggtga accaaatcgg cggtaaagag
     7741 cgttatttct ttggcaatat tttgtggcag acacgggcgt tgatggaccg cgcgatcggt
     7801 cataatctgg cgaaaggccg cccggagcgc gaatatttac agaccggcga tgcggtggat
     7861 agctggaaag tgattgtcgt tgaaccgaaa aagcaactta cgttgttatt tggcatgaaa
     7921 gcgccggggc tgggacgact gtgttttacc ctggaagata aaggtgacta tcgtactatc
     7981 gatgtccgcg ctttctggca tccgcacggt atgccggggc tgttttactg gttattgatg
     8041 atccccgcgc atctgtttat ttttcgcgga atggcaaaac aaatcgccag actggcagaa
     8101 caaagcacag attaatcgac aaaacctgcg ttattctttc atgtttccca ttgcatcatg
     8161 ggggaaatca cggaagaatg cgcacgtcat tttctcaaca acagtggatt gatatgaagg
     8221 tactggttac cggcgccacc agcggcttag gtcgaaacgc ggtagagttt ttatgccaga
     8281 aaggcatcag cgtgcgtgcg agcggtcgca acgaagcaat gggtaaattg ctggaaaaaa
     8341 tgggcgcaga gtttgttccg acggatctga ccgagctggt ttcatcacaa gctaaagtga
     8401 tgctcgcggg cattgatacg ctgtggcact gctccagctt tacctcaccc tgggggacac
     8461 aacaggcttt cgatctggct aacgttcgcg ccactcgccg cctgggtgaa tgggctgtcg
     8521 cctggggtgt gcgtaacttt attcatatct cttccccctc cctgtacttc gattatcacc
     8581 accatcgcaa tattaaagaa gattttcgcc ctcaccgctt cgccaacgag tttgcccgca
     8641 gcaaagcagc cagcgaagaa gtgatcaata tgctttcgca ggcgaatcca caaacgcgct
     8701 ttactattct gcgcccacaa agtctgttcg gaccgcacga taaagtcttt attccccgtc
     8761 tggcgcatat gatgcaccac tacggcagta ttctgttacc gcatggcggc agtgcgctgg
     8821 tcgatatgac ctactatgaa aatgccgtgc acgcaatgtg gctggcaagc caggaagcct
     8881 gcgataagct accttccggg cgtgtgtaca acatcaccaa cggcgagcat cgcacactgc
     8941 gcagcatcgt gcagaagctg atcgacgagt tgaatattga ctgtcgtatt cgttccgtcc
     9001 cttacccgat gctggatatg atcgcccgca gcatggagcg tttaggccgc aagtcagcaa
     9061 aagagccgcc gctgacccac tacggcgcct ccaagttgaa ttttgacttt acgctggata
     9121 ttacgcgtgc gcaggaagag ttaggttatc agccggtcat caccctggat gaaggcatcg
     9181 agaaaactgc cgcctggcta cgcgaccacg gaaaactgcc gcgctaatcc tgcccgtatt
     9241 tctccagcaa tgcttcggca atcgcctgag tttctgcatc cgcttcgccg ttatataacg
     9301 tcgggcggaa atgcatctgg aatgccataa tcacgcgccg ctgctcgcgc ggtgtcatat
     9361 caggttttac gtcataaccg tagcgcgcca aaagctccag caatgacgcg gtatctaccg
     9421 gtgtgtgcgg cgcgcgcccg gcaaggtaaa agttcacccg ctgcgcatcc ggccaggcac
     9481 caatcccctg ctgcgccagt tgctgccagg gaaataatgg ccccggatcg tctttgcgct
     9541 gcggtgcgat atccgcatgt gccactacgt tttccggctt gatgtgataa cgggcaataa
     9601 tatctttcgc cagcggaata agcgcctgaa tctgtgcctg ttcaaacggg gcaaaatatt
     9661 tcactccgga tgatttttgc cagccacggt tttccagctc aatgccaata gaggtgtcgt
     9721 taaggcgcgt tgccccgcgc caggcgctaa tccccgcatg ccaggccagt tcttgttccg
     9781 gcaccagttg ccagatgcgc ggtttaccgt tgtatcgcgg tggcaccgca gggatcagat
     9841 aatgcgagct gacctgctta tcagtcagtg tcgccagcga gctatcaaaa tcatctgcgg
     9901 tgtagtggat caccagcact ttaatgcgcg gatacgccgc ctgcgcctgg tgtcgggtat
     9961 caagctgata tccctctttc tcgacaatgc ctttttcgcc tgcacaccct gccagtaaca
    10021 gagcggcagc gaccagccag aagactcttc tcatcgacgc gttttcaccg ccgtaccgct
    10081 gacgctaacc atcagcatac tgccgttttg cccgaccgtt tcgtagtcga tatcaatacc
    10141 aactacggca tcggccccca gcgcccgcgc ctgggagcct aattcctcaa aggcgatctc
    10201 ccgtgcttta cgcagttctt tttcatacgc accggaacgt ccgccaacga tatcgcggat
    10261 accggcaaag aaatcacgga aaatattggc acctaaaatc gcttcgccgg tcaccacacc
    10321 gcaatattca acgatggtca ggccttccag agttggggtt gtagaaaatt gcatgatcga
    10381 tctccttgtt tgttaagcct tcgatctcta aagcattatc ggactgatat gccatgttgg
    10441 gaagaaggtt ttgatcagtt taggaaatca tctcattgtg tcaaaaacac ccacttaagt
    10501 ctttacgatt atccgttgca taacccgata ttggtttcat tatagggtac cgcttacctg
    10561 tcttattatg ttgcaccaac aacattaatg agcggaaaga ggcaacttat attgtaaatg
    10621 actcctaaag tcttccccat cttgtgactg aggtctatac cgctggattg ctttttggtc
    10681 agccgccgca aaacaagaaa gtaataagat ctcaatgctt ttgtcattaa tataagcgaa
    10741 gatcattctt gcaaactcgt atataaacgc ccgatcctga gcgcttaata gtttaataca
    10801 atatttattt ttaagagaga aaataatttc aaggaaagca tccgcagcaa ccttcacgtt
    10861 attcccggcg ttcatattat atatatccct tgcacagata gagtacagag atagtaaaca
    10921 gccatacaaa t
//
LOCUS       DAZSIO010000131        10927 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZSIO010000131 DAZSIO010000000
VERSION     DAZSIO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10927
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.131"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            157..504
                     /locus_tag="ACHX5T_003092"
                     /pseudo
     CDS             157..504
                     /locus_tag="ACHX5T_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            557..952
                     /locus_tag="ACHX5T_003093"
     CDS             557..952
                     /locus_tag="ACHX5T_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR8468178.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="ACHX5T_003094"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="ACHX5T_003094"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR8468179.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="ACHX5T_003095"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="ACHX5T_003095"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR8468180.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="ACHX5T_003096"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="ACHX5T_003096"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR8468181.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="ACHX5T_003097"
     CDS             complement(5323..6423)
                     /locus_tag="ACHX5T_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR8468182.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="ACHX5T_003098"
     CDS             complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="ACHX5T_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR8468183.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7571..8137)
                     /locus_tag="ACHX5T_003099"
     CDS             complement(7571..8137)
                     /locus_tag="ACHX5T_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR8468184.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8353..10086
                     /gene="argS"
                     /locus_tag="ACHX5T_003100"
     CDS             8353..10086
                     /gene="argS"
                     /locus_tag="ACHX5T_003100"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR8468185.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(10128..10172)
                     /locus_tag="ACHX5T_003101"
     CDS             complement(10128..10172)
                     /locus_tag="ACHX5T_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468186.1"
                     /translation="MSVFRIHLDGNKKA"
     gene            10263..10751
                     /locus_tag="ACHX5T_003102"
     CDS             10263..10751
                     /locus_tag="ACHX5T_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFR8468187.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2669 a         2789 c         2605 g         2864 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctatttg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     6541 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     6601 tttatcagtt ttagcaattt aaatcccata atcaatgtga atataaaaaa tatttttagc
     6661 acaacgctat gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg ttttttatac
     6721 tgctcaggga taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc
     6781 atattgggcg acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc
     6841 tttcatttca gcaaccgccg ccccgtctac gatataacgc gaatactcgt ccgcgtgttc
     6901 atctgatgac atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc
     6961 cgcggagcta tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg
     7021 gactcctgct ccagccataa tgattggagc atcacgatgg gcaataagtt ccataatttt
     7081 tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa
     7141 ttccgcaaga ttattgagtg tatataaagg gttagcgcac atatcgaagg cgcgatgaaa
     7201 agtcactgcc agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt
     7261 cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg
     7321 cacatcctca agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg
     7381 gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa
     7441 cgacggcgtt aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt
     7501 ttgctgcgcc gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt
     7561 tgctccttaa ttacgctgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa
     7621 acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct
     7681 caccttttgg tgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa
     7741 gcaatgccag cgcacgggca ttcagcgttt ctggatcacc cggcagaacg atttcaatat
     7801 gttcccaacc ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acattggaaa
     7861 actgccagtg cgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat
     7921 tgatcatttt ttctgacaaa agctcgccac actgttcaaa accgcgacgc cagcgctcag
     7981 cggtgacgtt ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca
     8041 accccagacg gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa
     8101 tatcctgcag ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa
     8161 aagcgttaat ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga
     8221 tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca
     8281 gtatactccc gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg
     8341 taaggtattc cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt
     8401 gcggcaggcg cgcctgcgga ttgcgaaccg cagattcgtc agtcagcaaa agttcagttc
     8461 ggcgactatc aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga
     8521 caattagcag agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag
     8581 atcgccggtc caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt
     8641 cagcaggcgc tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg
     8701 gttgactact ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc
     8761 attattggtg acacagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca
     8821 aaccacgtcg gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag
     8881 cagcaggaaa acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg
     8941 aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa
     9001 ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg
     9061 acgcagaacc agatcactta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg
     9121 ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt
     9181 ctggcagtag aaagcgaagg ggcgaccgtc gtattccttg atgtgtttaa aaacaaggaa
     9241 ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact
     9301 gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac
     9361 atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc
     9421 tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac
     9481 ggcaaaccgt tcaaaacccg cgcgggtggt accgtgaaac tggcagacct gctggatgaa
     9541 gccctggagc gtgcacgtcg tctggtggca gaaaagaacc cggatatgcc agctgacgag
     9601 ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa
     9661 aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc
     9721 gcgccataca tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa agcagaaatt
     9781 aacgaagagc aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg
     9841 gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg
     9901 catgtaatgt gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac
     9961 tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa
    10021 ctgacagcga agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt
    10081 atgtaatcga tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta
    10141 ttgccatcta aatgtattct gaaaacggac atgccattgt tttctcactg ttgagtaaga
    10201 ggccagaagc gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg
    10261 aagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag
    10321 atacggttat agtaaacgac catgacactt cagccatcaa agattgttgg caaaaaaatt
    10381 cagatgatga tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg
    10441 tcgatgcgca attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac
    10501 gcacaggtgc aaaaaaactt gatactgaac aacttaactt gcttaaaaaa tcacagcgag
    10561 cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag gatgttcagg
    10621 atttaagtga cccctattct gaatcagaat ggctatcatg tatgatcata cagaccaata
    10681 cgcgaactcg ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga
    10741 caagaggata attcacatct ttttggcatg ttttgttgca agctattcct gataaatgat
    10801 tgcaacaaaa catccagcct tttgactgag ttattaaaca tactcgcgag cgcgtaattt
    10861 tttgtctttc agcgataatt cacaatcact tcattccgtt gtacctttag cggtggaatt
    10921 aaccgcc
//
LOCUS       DAZSIO010000132        10917 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZSIO010000132 DAZSIO010000000
VERSION     DAZSIO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10917
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.132"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            108..722
                     /gene="evgA"
                     /locus_tag="ACHX5T_003103"
     CDS             108..722
                     /gene="evgA"
                     /locus_tag="ACHX5T_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR8468188.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            727..4320
                     /gene="evgS"
                     /locus_tag="ACHX5T_003104"
     CDS             727..4320
                     /gene="evgS"
                     /locus_tag="ACHX5T_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR8468189.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(4376..5521)
                     /gene="yfdE"
                     /locus_tag="ACHX5T_003105"
     CDS             complement(4376..5521)
                     /gene="yfdE"
                     /locus_tag="ACHX5T_003105"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR8468190.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5595..6539)
                     /gene="yfdV"
                     /locus_tag="ACHX5T_003106"
     CDS             complement(5595..6539)
                     /gene="yfdV"
                     /locus_tag="ACHX5T_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR8468191.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6609..8303)
                     /gene="oxc"
                     /locus_tag="ACHX5T_003107"
     CDS             complement(6609..8303)
                     /gene="oxc"
                     /locus_tag="ACHX5T_003107"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR8468192.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8357..9607)
                     /gene="frc"
                     /locus_tag="ACHX5T_003108"
     CDS             complement(8357..9607)
                     /gene="frc"
                     /locus_tag="ACHX5T_003108"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR8468193.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(10120..10743)
                     /locus_tag="ACHX5T_003109"
     CDS             complement(10120..10743)
                     /locus_tag="ACHX5T_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR8468194.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         3203 a         2457 c         2249 g         3008 t
ORIGIN      
        1 taaaggaaac tcagattttc ttatttttat tgagaaaatg agatgacgcc ttatgtctgt
       61 attactacag ggagaaggga gatgcttcat tgcaaaggga ataatctatg aacgcaataa
      121 ttattgatga ccatcctctt gctatcgcag caattcgtaa tttattgatc aaaaacgata
      181 ttgaaatctt agcggagtta actgaaggcg gaagcgccgt tcagcgggtg gaaacactta
      241 agcctgatat cgtcatcatt gatgtcgata tccccggagt taacggtatc caggtgttag
      301 aaacgctgag gaagcgccaa tatagcggaa ttattattat cgtctccgct aaaaatgacc
      361 atttttacgg gaaacattgt gctgatgctg gcgctaatgg tttcgtgagt aaaaaagaag
      421 gcatgaacaa tatcattgcg gctattgaag ctgcaaaaaa tggctactgc tatttcccct
      481 tctctctcaa ccggtttgtt ggaagtttaa cgtccgacca gcaaaaactc gactccttat
      541 cgaaacaaga aattagtgtc atgcggtata ttcttgatgg caaggataat aatgacattg
      601 cggaaaaaat gttcatcagc aacaaaactg tcagcacgta taaaagtcgc ctgatggaaa
      661 aattagaatg taaatcactg atggatcttt acacattcgc gcaacgtaac aaaatcggct
      721 aatcacatga agtttttacc ctattttttt cttctctgtt gtggtctttg gtcgaccata
      781 agtttcgcag acgaagatta catcgaatat cgtggcatca gtagtaacaa ccgtgtcaca
      841 cttgatccag tacgtctgag caacaaggaa ttacgttggt tagcgagcaa aaaaaatctt
      901 gtgattgcag tacacaagtc ccaaacggct accttgttgc ataccgattc gcagcaacag
      961 gttcgtggta ttaatgctga ttatttaaat cttttaaaaa gagcgttaaa tatcaaatta
     1021 acactccggg aatacgcaga tcatcaaaaa gcaatggacg cgcttgcaga aggtgaagtc
     1081 gatatagtgt tatcacattt agttacttcg ccgcctctta ataatgacat tgctgcaacc
     1141 aaaccattga taattacctt tccggcgctg gtaaccaccc ttcacgactc aatgcgaccg
     1201 cttacctcac caaaaccagt aaatattgct cgggtagcaa attacccccc agacgaggta
     1261 attcatcaat catttccaaa agcaacaatt atctctttta caaatttata tcaggcatta
     1321 gcatccgtct cagctgggca caatgattac tttattggta gtaacatcat taccagcagt
     1381 atgatttccc gctatttcac tcactcctta aatgtagtga aatattataa ctcgccgcgt
     1441 caatataatt ttttcttgac cagaaaagaa tctgtcattc ttaatgaagt actcaataga
     1501 tttgttgatg ctttaacaaa tgaagttcgc tatgaagtat cacaaaattg gcttgataca
     1561 ggaaacctgg cctttctgaa caaaccatta gaactcactg aacatgaaaa acagtggatt
     1621 aagcagcatc ccaatttaaa ggtgctggaa aatccttact cgccccccta ttctatgacg
     1681 gatgaaaatg gctcggttcg gggcgttatg ggggacattc ttaatattat taccttgcaa
     1741 acaggtttaa atttttctcc gatcaccgtt tcacacaata tccatgctgg aacacaactt
     1801 agccccggag gatgggatat aatacctggc gctatttata gtgaagatcg agaaaataat
     1861 gttttatttg ctgaagcctt cataacaacg ccttacgttt ttgtcatgca aaaagcgcct
     1921 gacagtgaac aaacattaaa aaaaggaatg aaagttgcca ttccatatta ttatgagctg
     1981 cattcgcaat taaaagagat gtatccggag gttgaatgga tacaggtcga taatgccagc
     2041 gctgcatttc acaagattaa ggaaggtgaa cttgatgctc tggtcgcgac acagctaaat
     2101 tcgcgttaca tgatcgatca ttactatcct aatgaacttt atcattttct tattcctggc
     2161 gttccgaatg catcgctttc gttcgctttt cctcgcggag aaccggaact taaggatatt
     2221 attaataaag cactgaatgc aattccccca agcgaagttc tgcgcctgac ggaaaaatgg
     2281 attaaaatgc ccaatgtgac cattgacaca tgggacctat atagcgagca attttatatt
     2341 gttacgacat tatccgtttt attagttggc agtagccttt tatggggatt ctacctgtta
     2401 cgctcagttc gtcgtcgtaa agtcattcag ggtgatttag aaaaccaaat atcattccga
     2461 aaagcactct cggattcctt accgaatcca acttatgttg taaactggca aggtaatgtc
     2521 attagtcata atagtgcttt tgaacattat ttcactgcgg attactacaa aaatgcaatg
     2581 ttaccattag aaaacagtga ttcacccttt aaagatgttt tttctaatgc gcatgaagtc
     2641 acagcagaaa cgaaagaaaa tcgaacaata tacacacagg tatttgaaat tgataatggc
     2701 atcgagaaaa gatgcattaa tcactggcat acattatgca atcttcctgc aagtgacaat
     2761 gcagtatata tttgtggttg gcaagatatt actgaaacgc gtgatctaat taatgcactc
     2821 gaggtagaaa aaaataaagc gataaaggct accgtagcaa aaagtcagtt tctggcaacg
     2881 atgagtcacg aaataagaac accaataagc tctattatgg gcttcctgga acttctgtcg
     2941 ggttctggtc ttagcaagga gcaacgggtg gaggcgattt cacttgccta cgccaccgga
     3001 caatcactcc tcggcttaat tggtgaaatc cttgatgtcg acaaaattga atcgggtaac
     3061 tatcaacttc aaccacaatg ggtcgatatc cctactttag tccagaacac ttgtcactct
     3121 ttcggtgcga ttgctgcaag caaatcgatc gcattaagtt gcagcagtac atttcctgat
     3181 cattacctgg ttaagatcga ccctcaggcg tttaagcagg tcttatcaaa tttactgagt
     3241 aatgctctca aatttaccac cgagggggca gtaaaaatta cgacctccct gggtcacatt
     3301 gatgacaacc acgctgttat caaaatgacg attatggatt ctggaagtgg attatcgcag
     3361 gaagaacaac aacaactgtt taagcgctac agccaaacaa gtgcaggtcg tcagcaaaca
     3421 ggttctggtt taggcttaat gatctgcaaa gaattaatta aaaatatgca gggcgattta
     3481 tcattagaaa gtcatccagg cataggaaca acatttacga tcacaatccc ggtagaaatt
     3541 agccagcaag tggcgactgt cgaggcaaaa gcagaacaac ccatcacact acctgaaaag
     3601 ttgagcatat taatcgcgga tgatcatccg accaacaggc tattactcaa acgccagcta
     3661 aatctattag gatatgatgt tgatgaagcc actgatggtg tgcaagcgct acacaaagtc
     3721 agtatgcaac attatgatct gcttattact gacgttaata tgccgaatat ggatggtttt
     3781 gagttgactc gcaaactccg tgagcaaaat tcttccttac ccatctgggg gcttacagcc
     3841 aacgcacagg ctaacgaacg tgaaaaaggg ttaagttgcg gcatgaactt atgtttgttc
     3901 aaaccgttga ccctggatgt actgaaaaca catttaagtc agttacacca ggttgcgcat
     3961 attgcacctc agtatcgcca ccttgatatc gaagccctga aaaataatac ggcgaatgat
     4021 ctacaactga tgcaggagat tctcatgact ttccagcatg aaacgcataa agatctaccc
     4081 gctgcgtttc atgcactaga agctggcgat aacagaactt tccatcagtg tattcatcgc
     4141 atccacggtg cggctaacat cctgaatttg caaaagttga ttaatattag ccatcagtta
     4201 gaaataacac ctgcttcaga tgacagtaag cctgaaattc ttcagctgct gaactctgtt
     4261 aaagaacaca ttgcagagct ggaccaggag attgctgttt tctgtcagaa aaatgactaa
     4321 attgcggctc ccacaatgtt ctaatgtggg agctatttac cagcacatct tacatttatg
     4381 atgagaactc ctggcgaatt tgttccccat gctggtcgag cgttgccgct cccggcataa
     4441 catgcgggtc cgcgcagccg ctgattttta tcggattacc cggcatcatt attcctccgg
     4501 cttcaatcaa catatttctc gcctgagttt gtggcaaatt aatggcctca gccacactta
     4561 ataacggcgc gacgggcaca ccaacttcat gtattttggc taaccaaact tcagctgcct
     4621 gcgtttttaa cgttcgctca atatattgtt taagaatagc ctggttttgt acgcgtaaaa
     4681 tattgctgct aaatcgggga tcattaacca gttccgtaag ctccagtgcc tggcataacg
     4741 cagaaaaaag cttgtcatta ccacaacaaa tcgtaatcgg cttatcctga gtatcgaaaa
     4801 catcaaaagg tgccatgtag ggatggcgat ttcccaggcg ttgtggtgac ttccctgtcg
     4861 caatatatgc catcagacca tgctccagaa aactcagcgt ggcatcaaac atcgctatat
     4921 cgacatgcgc ccctctctgg ctcttttcgc ggccataaag tgcactcact attccgctga
     4981 ataaatagac accgccgcat agatccgcaa gagaggtacc aacacgcact ggcggagcat
     5041 cagggtaccc tgtttccatc attatcccac tcattgcctg aatgatggta tcgtaggcag
     5101 gagcatcttt tagcggaccg gtatgtccga aacctgacga tgaagcatat atgaggcgcg
     5161 ggttgatttc ttgtaacctt tcccatgaaa accccagttt ttccattgta cctaggcgaa
     5221 aattctcagc taatacatca gcttgtttga gcatatttat aaatatactt ttatcgtgat
     5281 cattctttaa atcaagaacc acactctctt tgccatgatt aataaaactg taatagagtg
     5341 actgtccatc cacataggga ccaaatgtgc gggtatcatc accatgacct ggtggctcaa
     5401 ctttaattac ccttgcgccc atattataaa gaagttgagt tccgaaaggt ccattaagga
     5461 catgtgtcat atcgataact aataagcctt caaacggccc tttgctttca ttatttgtca
     5521 tttgcttatg ccttgaaaaa agccgaaggt gaaaagacac ttcggcattt atgtaaaatc
     5581 aataacatga ttaattatga aaccagtcga ctgacataaa tccacaaggg agccgtgacg
     5641 acaaagccca gtacgctcac agccaatgac gctgtaccag tacgggtgta gacattaaac
     5701 cggctggcaa taatgattcc ggagaatgcc ggcggtaatg cgcctgccag taccatcatc
     5761 tgcaaatgtt cgctgttcaa atgacatgcc ataccaacga gaagcagcgc cagtggcatc
     5821 agaatcaact tcaggaaggt gttataagca atttcggcac tgaactcgaa tttatgtgca
     5881 gccagagtca accccgcagc gaataccgct acccctgagt tagccttcgc aatcagatta
     5941 aaggttgggt cccatgctgc cggaattttt accccaacca gcaccaggat cgttgccaga
     6001 acaggtgccc atactactgg ctcctttgcc gcagaaatta atgcgctcag attactattc
     6061 ttcttaccat ccgctcctga ggaaggattc agcaaataca gaccaatagg aatagtaatt
     6121 gcgttaacaa taatagaaat aattgctacc actaaacctg ttgatacgga atcaccataa
     6181 ataggatcga gaactgcaaa ccccaggaat ccaatggtag gtgaacccgc aattaatgca
     6241 catacagctg cttctgcatg ggtacgttta aaaaatttgt agcaaccaaa ccaggagaaa
     6301 aagaaacatc cgacaataac cacaagtgat acaagggtca gacgagtgtc cgcaaaaatc
     6361 atttccctgt ttgcccgggt aatagaaaca aatagagccg caggaagagc gtagtttaat
     6421 accagtttat taaatgcgcg ggcttgatct tctgaaaaag tttctcgtct gccgctaaaa
     6481 taacccaaca gcatgattac gataataggc aataaatcgc caataaaaaa tgttagcata
     6541 atagtatttc cgataagggt cccctcacca tcgcataatg agttggcaag aggagacgat
     6601 gtcagtattt aattaccagc gacttgtttt gggttaagtt tggtaatatg gccactttca
     6661 gttcctgctg cagggtcgat aaccacatta ataatggtcg gtttgcgcga ctgaataccg
     6721 gtggttaaag catgacgaag ttcatctgtc gtggtgacgt tatagccaac gccacgaaac
     6781 gcatccatta atttgtcata ccttgcatgg tgcaacagat ccgttggtga tggtgcacca
     6841 gcgccactga gatcaacacc gtctcctctg tagatgccgc cattattaaa aataacgatc
     6901 gtcaccggca ggttatatcg acaaatcgtt tcaatttcca tcccactgaa accaaaagca
     6961 ctatcacctt caatggcgac aaccggagaa ccagaagtca cgctagcacc gatggcatag
     7021 cccataccga tgcccatgac accccaggta ccacaatcca gacgacgacg tggtttatac
     7081 atatcaataa tatttcgtgc attatccagg gtatttgcac cttcattaac taaataaata
     7141 tcctggttct cgcgcaatac gtcgcgcaca gcacttaatg cattaaagta atttaatggt
     7201 tgagtatctg tacttaattt ttcatgcatt ttttgtgcat tttgctgctt gtggatattt
     7261 aaaatatcgc gccataccag tggagtcgta aatgtgtttt gtttcagttc tgccagcata
     7321 ccttgcatac tggatgcaat atcaccaacg actggcacag caatggggcg gttgctgtca
     7381 atttcctgcg gttcaatatc cagttgaata aactgtgtat ctgccgccca tcctttttta
     7441 ccgtgtgcca ataaccaatt cagtcgtgca ccaacaagca tgacaacgtc agcatttgcc
     7501 agggcaaacg aacgcgcagc tgccgcagaa agtggatgcg tatcttcaag gatccctttc
     7561 gccatagaca ttggcaggaa tggaatctga gcactttcaa taaattcacg aagctgttca
     7621 tcagcttgtg aatacgccgc gcctttgcca aggataatta atggccgttc agcttttgct
     7681 aaaagcgaaa ttgcgctagt gactgacttc gggcatggca ataatgctgg cgacggattt
     7741 tcaactttaa caatcgtggt taacgcttcg tctttttcca tcgtcgcggc caggacattt
     7801 gctggcaaat caagataaac tccgccaggg cgacccgata cagagacccg gatagcgcgt
     7861 gccaatgcaa tgccaagatc ctgcggctga ttaacgcgaa atgctgcttt ggcatacggt
     7921 tttgccgcat tcatttggtc cagctcttca taatcacctt gctgtaggtc gacgatcgcg
     7981 cggtcgctgg agccgctaat catgataatc ggaaaaccat ttaccgttgc gttggccaat
     8041 gcggtcaaac cattgaggaa tcctggcgca gaaactgtca ggcagatccc cggtttttgg
     8101 gtaagaaaac cgcttgccgc agcggcatag cctgccgact gctcatgacg aaaaccaata
     8161 taacgaatgc cttccgcctg ggcatggcgt gccatatccg tcacaggaat acctacaaca
     8221 ccataaatag tgtcaatatt attctgtttt aatgcttcaa cgatgatatg cataccatct
     8281 gtcatttgaa gttgatctga catgattaat aacctcgaaa tttttataaa ttgggagcca
     8341 gaagcccccg ttaatatcag atggcgtggt tttgcttcat tgcagcaatt tcatcgtcgc
     8401 tataacccag ctcctgcaat acagcagcgg tatgttcacc taatagcggc gcagctttaa
     8461 tatccggcgt aaaggcagag aatttcattg gacaaccaac ggtcagatat tttccacgca
     8521 acggttgttc cacttcaaca acactgccac tttggcgcaa agagggatca agtgaaattt
     8581 ctttcatact taaaaccggt gcacaaggaa tatcaaactg agtcaaatag gccaccgctt
     8641 catgtttatc aatagtgaca gtgtattttt cgatttcagc aaaaatatcg aaaatatgtg
     8701 gctgtcgggc atgggctgta ctgtatgccg gatcggtaat ccattctggt ttgccgatgg
     8761 ctttacaggt gttttcccag ttttgctcct gaatagtgaa ataaatatag gcgttaggat
     8821 cggtttccca gcctttacat ttcaggatcc agccaggctg accgccacca cctgcattac
     8881 caccgcgggg aactgcatca ccaaatgtac cattcggata ctgcgggtat tcttccagat
     8941 aacccaattt atcgagacgc tgctggtcac gtaatttcac gcggcaaagg ttcaatacgg
     9001 catcctgcat tgacatggtg actcgttgcc cacgccccgt tttttcgcga tgcagcaaag
     9061 cagcaagtaa accgatcagc aaatgcattc cggtgttgct gtcacccaac gctgcagcgc
     9121 ttaccagcgg cggaccatcc caaaaacccg tagtggatgc cgcgccaccc gctgcctgag
     9181 caacgttttc ataggctttt acattcacat aaggcgaaca ctcatcaaac cctttgatcg
     9241 aaccaaaaat cagacgtgga ttgatttctt gaatatgctc ccaggtgaag cccatgtgat
     9301 caatggcccc tggatgaaag ttctcgacta agatatcagc ttcgcggatc agcttttcca
     9361 ttacctcttt gccttccgct gttttggtat ttaactcaat agaacgtttg ttactgttaa
     9421 gcatggtgaa gtaaagcgca tcgatatcag gaatatctcg cagttggtga cgcgttacgt
     9481 caccaacgcc gggacgttca attttaataa cgtcagcgcc aaaccaggcc agcatttgag
     9541 tacaagatgg gccagattgc acaccggtga aatcgagaac tttaattcct tgaagtggag
     9601 ttgacatagc aatacctatt agttaattaa atatgtgtta atttagcgtt tatttaaggc
     9661 ataacgccct ctataaataa tctgcaaaat atcttcgtag cggcactcga tatttttaaa
     9721 ttcctgtatc aagtactgaa aattatttta tcgccacttc caattccttt ctgcatggta
     9781 agtagtgagg acgaaaagaa gaatatgata aatattaatt cgtggtaatc agagagagtc
     9841 gcgacatttg tgatgttatt cttacaactg taagaagcgc cggtgattta caagtaagag
     9901 tatgattacg ctttttaaag cgtaccataa agcataacat aataaatgat ttgtgatgac
     9961 acgggattat tgcggtttac tttgaggaaa ataaaatgcg tgtttataga accgcgagcg
    10021 ccaaataatt ttatgaccac aacctgtcgt attgtggtca taaaaaattg tcggctggag
    10081 ggagttaaat cctttacctc aatatactgc ctgtcagcat tagttcacaa acagtgcttc
    10141 gctatcgacg atgattccat cttccgcacc tttaagcgcc aggtttgcct cgtagtattg
    10201 ctttttatca agcagtttct gcgcatcagc aagtgcatta cgagtctgtt taagcggcat
    10261 cagatattgg ttttccatta cgccaacacc ggccaggcga agctcttcca ttgcgccctt
    10321 tttatcccct ttcgccattt tttcattggc aatttttatc gcggcttctt tttcgggtgt
    10381 tgctacataa ctctcagaaa ttccgacaga tgcattaatg acaatatatt ggtcatcatt
    10441 gagattggtc tttttacctg gcttagcgaa ctttgcccat tcggtactgt catctgacag
    10501 caaagcggaa gcttcattgg tcagttcttt tgccttttcc ggatcgccat gaaacagagc
    10561 caggcgcgct acctgcacgt cacgcatcgc atataaacct tgttcagaga tacgctctgc
    10621 tgctgccgtt ttctgggttt gctgagtttg ctgttgtgcc gctaccggtg cattatcagc
    10681 agcccataca gttgaagatg ccagaattgc tgtgaccatc gtagccataa ttaaacgttt
    10741 catacttacc tcattcttta acagttttag aaaacgccgc catcatattg ggtgagcatg
    10801 aaatgagaaa tcagagagca acggtcattt tgaaagggtt atcttacaat tgtagttatt
    10861 atcgcaaagc ggcatttagt cttacagttg taggaatatg cctcctgtcg cagagaa
//
LOCUS       DAZSIO010000133        10820 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZSIO010000133 DAZSIO010000000
VERSION     DAZSIO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10820
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.133"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            181..1011
                     /locus_tag="ACHX5T_003110"
     CDS             181..1011
                     /locus_tag="ACHX5T_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFR8468195.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1152..1925)
                     /gene="uxuR"
                     /locus_tag="ACHX5T_003111"
     CDS             complement(1152..1925)
                     /gene="uxuR"
                     /locus_tag="ACHX5T_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR8468196.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2140..3600)
                     /locus_tag="ACHX5T_003112"
     CDS             complement(2140..3600)
                     /locus_tag="ACHX5T_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR8468197.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3681..4865)
                     /gene="uxuA"
                     /locus_tag="ACHX5T_003113"
     CDS             complement(3681..4865)
                     /gene="uxuA"
                     /locus_tag="ACHX5T_003113"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR8468198.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            5205..6548
                     /gene="gntP"
                     /locus_tag="ACHX5T_003114"
     CDS             5205..6548
                     /gene="gntP"
                     /locus_tag="ACHX5T_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR8468199.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6799..7701)
                     /gene="fimH"
                     /locus_tag="ACHX5T_003115"
     CDS             complement(6799..7701)
                     /gene="fimH"
                     /locus_tag="ACHX5T_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR8468200.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7721..8164)
                     /locus_tag="ACHX5T_003116"
     CDS             complement(7721..8164)
                     /locus_tag="ACHX5T_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8468201.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(8237..8767)
                     /gene="fimF"
                     /locus_tag="ACHX5T_003117"
     CDS             complement(8237..8767)
                     /gene="fimF"
                     /locus_tag="ACHX5T_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFR8468202.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8777..>10820)
                     /gene="fimD"
                     /locus_tag="ACHX5T_003118"
     CDS             complement(8777..>10820)
                     /gene="fimD"
                     /locus_tag="ACHX5T_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR8468203.1"
                     /translation="RIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNK
                     WQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV
                     IHGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIF
                     TVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQL
                     ADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNI
                     QLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLT
                     VTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGR
                     DQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLS
                     YSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHAN
                     GVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNT
                     LADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQS
                     SGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2596 a         2792 c         2656 g         2776 t
ORIGIN      
        1 ataattttaa atgagcgttt tttgatactt tagagtgttt cctacccttt aatatcactg
       61 tgtagagttg atatcactac aacagaaaca tgcatgtcat ttgtttactt ctctttttac
      121 tacagaaaaa tttatctatt tttctaaaca tcagttaaat actcataaaa ggtaactatt
      181 atgtctatgc cattaagcaa tgccgctaat aaccaattca atacaaataa tcacttcctt
      241 catcatccaa aagtcgacag tgagttaaca cgtcgatatg aatatgcacg cctggatacg
      301 gaaaatatct atcttctgcc tcttgctcgt ggcaataacc ataattacga tggaaaaagc
      361 gttgtcgaaa ttagaaaact taatattggc aaacagtcct ggccatttaa ttatgtgaca
      421 gatacatgca gagaattcaa tggtattaca acaacaggac gtatgctcta tcgaaacttg
      481 aaaatcacct cagctttaga cgaaatatat ggtggtatat gtaaaaaggc gcatgctgct
      541 acagagttag cagaaggttt acggctaaat ttatttatga agagcccttt cgatcctgtt
      601 gaggactata cagttcatga aataacatta ggccctggtt gcaatgttcc tggttatgcc
      661 ggaaccacga taggatatat ttcaaccctt ccagcttctc aggcaaaaag atggaccaat
      721 gagcagccaa gaattgatat ctatattgat cagataataa ctgtctcagg ggtagcaaat
      781 tcttccggat tcgcccttgc cgcattactt aatgctaata ttgagatggg aaatgaccca
      841 atcattggta tagaagccta tcctggtacc gcggagatcc attcaaaaat gggatataac
      901 gttatccccg gtgatgaaga tgcgccactc aaacgcatga ctctgcaacc gtcatctctt
      961 ccagaactgt ttgaattaaa aaatggtgaa tggaattata ttggtaaata gccactgaga
     1021 agaatgagta ctaatattca ggttatacag atatttaaac aaaaccctgc aatttacaaa
     1081 gtaatactca ttgcgacttc ttaatttaag aaagcgcaag gaacgtttac ccttgcgctt
     1141 attataataa gtcaggcgtc gactttatcc agcggccatg aatcaaagag atagccgtca
     1201 aaataaatat cgtcaacgtt cgagaattcc agcagacgtt gcttaacgtt ttccagatgc
     1261 tgccacattg ccagcttcgc cgctcgggca tcttttttga ttaacgcggc gaggatctgt
     1321 ttgtgatcgc ccaaccactc tttgcgatac aggctgtcat ccagatggct gtgcaactgg
     1381 atccacattg gattgttttc ccgccactgc caggactgac ggaataactc caccagcatg
     1441 ctgttatgcg ttgcttcagc aatggcgaga tggaactgca tgtcaccgct ttcgctgcta
     1501 cccggcgcgc tggaagccag ttcacgctct tccagttgca atgcctgacg cattttgacg
     1561 atatcttcgc gggtagcctg cagtgcggca aactcggcga tgttactttc cagtaactga
     1621 cgcgcctgta acagctcaaa aggacccgca tcattgcaga cgttggcatc cggactgtct
     1681 gtgttgtggc tgcctgagct atcaagcaca tagataccgg caccccggcg cacttccacc
     1741 agccctttga tctccagcat gatcagcgct tcacgtacca ccgtccgcgt gacatcaagc
     1801 atttctgcaa tttcacgctc cggcggcagc cgttcgccag gattgtacgg cgtctttatg
     1861 atcagatcgt ggatcatcgc cccgacttcc tggtaaggtc tttgcgcaga ggtgacagat
     1921 ttcatggtgt tgtgtccgtt aggtgcggtt aatcaaattt ttgccggatg cggcgtgaac
     1981 gccttatccg gcctacaacg ggcaatttta cggcccccgt taatctcaaa ctataccatt
     2041 agcactggaa gttggcacgt acaggtgggc aaaaaaacga aaactggtca accaaatatc
     2101 agtttgattg accagtaagt ctgtagggta atcagttagt tacagcgcag ccacacactc
     2161 gcgcgcaccg cgttcgcaca gctgctgata tgccgacgtc accgcgccaa caaagtcggc
     2221 attctgcggc agatcatcgg caaaaatacc gctcaggccc agcagcgctt tcacgcggtc
     2281 tgcgccctga tactgcgcgt taatcttctg gaactcggcc agcatcgggt cgaccacgtc
     2341 aatggcatta ccctgctcat ccacgccctg ggtgtaaccc atccagccag ccacgcccag
     2401 cgccaggtga cgccagctgc cgccgttttg caggtgcaga cgcaccgggt ccagtagacg
     2461 ctgcggtaac ttctggctgc cgtccatcgc aatctgccag gtacggtgac gcagagacgg
     2521 gttgctgaaa cgctcgatca gcagcgtcgc ataggcgttc aggtccgtac cttccggcat
     2581 cgacagcgtt ggcgcttgtt cctgcatcat cagggcaaag gccgctttgc gataatccgg
     2641 gttagtcatg gtgtcggcaa tggtttcata gccgccgagg taaccgaggt acgccaggaa
     2701 agagtggctg ccgttcagca tacgcagctt catcatttcg aacggcacaa cgtctgcaac
     2761 gaactgtgtg cccactttat cccagtccgg gcgaccatta acgaagttat cttcaatcac
     2821 ccactgacgg aacggttcgc aggcaatggc gcacgggtcg taaacaccca gctggtcagc
     2881 aatttcctgt aaggtttctg gcgtcgccgc cggaacgatg cggtcaacca tggtgcacgg
     2941 gaaggtgacg ttctcttcaa tccatgccgc tagctgcggg tcacgcgcct gagccagccc
     3001 cagtaccgcg accttcgcca catggccgtt ttcacgcacg ttatcgcagg acatcaccgt
     3061 aaatgctttc agcccttttt cacgacgcag acgcaaggct tcgacgatgt aaccaatcgc
     3121 ggacttcggc gcagtcgggt tttccagatc gtgcttgatc agcgggttat tgagatccag
     3181 ttgaccgctt gccgcatcag cgcagtagcc tttttccgtg accgttagag agacaatcgc
     3241 cgtttgcgga cgcgccatcg cgttgagaat accttcgcag ccgtcgattt ccggatgcag
     3301 cgcttctttc atcgaaccga taattttcag ctcggtgctt tctgcgcctt tttccgctac
     3361 ggtgtacagc agttgctgtt ttttcaggtt ttcgatcagc atgcggtcgt tgcctggcat
     3421 caggttaact tcgcagatgc cccagtcgct gtcggtgctt tccagcagat gatgggtata
     3481 cagcgcctgg tgcgcgcggt gaaacgcccc gcaaccgaga tgcacaatgc gtgattccag
     3541 acgagaatga tcccatgacg ggcgggcaac cggcagattg ctgtcaacaa tagtagtcat
     3601 cgtaaactcc agaatccgtc ttcagagagt gacggcaagg aagaaggaac cggcacggcg
     3661 cgcagccatg ccggtgggta ttaacggata aagaaagcgc gctggatcgc cagttcgaca
     3721 ccgcgaactt cggccaggcc tttcagacga ccaattgcgg agtaaccagg gttggttttc
     3781 ttcttcaggt cgtccagcat ctgatgaccg tggtccggac gcatcgggat caggtcttct
     3841 ttgccttccg ctttacgacg gtgttcttct tcaacaatcg ctttcaccac tttgtacata
     3901 tcaacgtcac cgttcaggtg cgccgcttcg tggaaggttt tcgggttatc ttcacgcatg
     3961 gtggagcgca gatgggtgaa gtaaatacgc ggaccgaact gcttgatcat atcaaccaga
     4021 tcgttgtcag cacgcacgcc gtaggaaccg gtgcacatgg tgaaaccgtt cgccatgctg
     4081 tttacggtat caaccatcca ctgcatatct tcaatggtgg aaacaatgcg cggcaagccg
     4141 aggatcgggc gcggcggatc gtccgggtga accgccatac gcacgccaac ttcttcagca
     4201 actggaataa tcgctttcag gaagacggca aagttttcgc gcagtttggc tttgtcgata
     4261 tctttgtaca gctccaggtg cttacggaac tggtcgaggg tatacccttc ttccgcgccc
     4321 ggcagaccgg caatgatgtt acgcgtcaga cgcgctttgt cttcgtcgct catagtggcg
     4381 aagcgttcag ctgcctgagc aatttcttct tcggtgtaat ccgcttccgc gcctggacgt
     4441 ttcaggatat gcatttcgaa tgcagcgaat tcgatctggt cgaagcgcag agctttggag
     4501 ccgtctggca gcacgtattc gaggtcagta cgggtccagt cgagcaccgg catgaagttg
     4561 tagcacacgg tgcgaatgcc acactgcgcc aggttgcgca gagtctgctg atagttagcg
     4621 atccactgct catagttgcc agtgtgggtt ttgatatctt cgtgaattgg tacgctttct
     4681 acgacagacc acaccaggcc tgcgtcttca acgatcgcct tgcgtttgag gatctcttct
     4741 acggaccata cttcgccgtt cgggatatgg tgcagcgcgg taaccacgcc agttgcgccc
     4801 gcctgacgga catcagctaa agaaaccgga tcgtttgggc cgtaccagcg ccaggtctgt
     4861 tccatgattt ttcctctttc acatagtggg ttccacagca aagagacgcg gaaccttgga
     4921 gttgtttagg tatccggatg aacaaccaga ccgcagccgt ggcgttgcat cggatgagat
     4981 ggcgtataag ttctaccttt tggtaagaca tgtcaaccag aattctgaaa ttggttaacc
     5041 gcgtcgcaag aatttcactc tcatggttgt tgctgcaaat tgcagcaaag ggtaatcgat
     5101 ggttattgat cagaggatgt gacattcatc gcaacaatgg ttgaccaatt tacataacat
     5161 gtcgcccaaa ataacaacgg ttcaaccaag taaggtaaaa cgttatgcat gtgcttaaca
     5221 ttctctgggt ggtattcggc attggcctga tgctgatact gaatttgaag ttcaaaatca
     5281 attcaatggt ggctttgttg gtggcggcgc tgtccgtcgg gatgctggca ggcatggatt
     5341 tgatgtcgct gctgcacacc atgaaagcgg gcttcggcaa cacgctggga gaactggcta
     5401 tcatcgtggt gttcggtgcg gtcatcggta aattgatggt cgactccggc gcggctcacc
     5461 agatagcaca tacgctgctg gcgcgtctcg gtctgcgcta tgtacagctg tcggtgatta
     5521 tcatcggcct tattttcggt ctggcgatgt tctatgaagt ggcctttatc atgttagcgc
     5581 cgctggttat tgttattgcc gccgaagcta aaattccgtt cctgaaactg gcgatcccgg
     5641 cagtagcagc tgccactacc gcacattcac tgttcccacc gcagccgggt ccggtggcgc
     5701 tggtgaatgc ttatggcgcg gatatgggga tggtttatat ctatggcgta ctggtgacga
     5761 tcccaagtgt aatctgcgca ggtctgatcc tgccgaagtt cctcggcaat cttgagcgcc
     5821 caacgccatc attcctgaaa gcagatcaac cggtagatat gaataatctg ccctctttcg
     5881 gcgtttcgat tctggtgccg ctgatcccgg cgatcattat gatctctacc accatcgcca
     5941 atatctggct ggtaaaagat acccctgcct gggaagtggt taactttatt ggttcctcgc
     6001 cgattgcgat gtttattgcg atggtggttg cattcgtact ctttggcacc gcgcgtggtc
     6061 atgacatgca gtgggtgatg aacgcttttg aaagcgcggt gaagagtatt gcaatggtga
     6121 ttctgatcat cggtgcgggt ggcgtgctga agcagaccat catcgacacc ggcattggcg
     6181 acaccatcgg catgttgatg tcccacggca atatctcgcc ctacattatg gcatggctga
     6241 tcactgtgct aattcgtctg gcgacgggtc agggtgtcgt ttcggcgatg accgccgccg
     6301 ggattatcag tgctgcaatc cttgatccag caaccggtca gctggttggc gtgaatccgg
     6361 cgctgctggt actggcgacg gctgcgggtt ccaacaccct cacccacatt aatgatgcat
     6421 ctttctggtt gttcaaaggt tactttgacc tgtcagtaaa agacacgttg aaaacctggg
     6481 gtctgctgga gctggtcaac tccgtggttg ggctgattat tgtgctgatt attagcatgg
     6541 tagcgtaaaa gaacaccaga gcctgccaat ggcaggctca gactgatgac taaagtctaa
     6601 aaatgtgccc ggacagtccc ctcgcccctc cggggagagg gttagggtga ggggaacaga
     6661 ccagtaaagt gcgctttgtt tatgccagat gcgacgctga cgcgtcttat ctggcctaca
     6721 aagggctaac gtgcaggttt tgtaggtctg atagcgtagc gcctcaggta ccagcattag
     6781 caatgcgctg taattttttt attgataaac aaaagtcacg ccaataatcg attgcacatt
     6841 ccctgcagtc acctgccctc cggtacgtgc gtaatttgcc gttaatccca gacttaccgc
     6901 cgaagttcct actgctccta acgataccgt gttattcgct ggaataatcg taccgttgcg
     6961 cgtcaactgt acgccgacgc cctgcgctgg tgaaaacgac gcggtattgg tgaaaatcga
     7021 gttgcccgca tctgcggttg tgccggagag gtaatacccc aggttttggc ttttcgcaca
     7081 ataaacggta agaggaattg gcactgaacc agggtagtcc ggcagagtaa cggtgacatc
     7141 acgagcagaa acatcacaac cgccagtggg caccaccaca tcattattgg cgtaaatatt
     7201 ccacacaaac tgaaaatcat cgctgttata gttgttggtc tgtcgcaaaa taagcacggc
     7261 aattaatgaa ccagctttaa tagccacccc gcccgcactg ctcacaggcg tcaaataaag
     7321 cgccaccggc cacggcttat ccgttctcga attataaaca acccgcggcg tttcgctggt
     7381 ggtcggaaat ggatagctac tgccactata ttttacggtc ccggaaaaat tagataacac
     7441 gccgccgtaa gccgagcctc gttgcagtgt gacatagtct gtaatggttt ccggataatc
     7501 gttatggcaa aagatttgcg tcgaaagatc tacgaccagg ttttgcccca cattcacgac
     7561 aggcgcaagg tttacataaa cattagcgct gccaccgcca atagggatag cggtaccatt
     7621 ggcggtttta caggcgaatg accaggcatt taccgaccag cccatcagca gtacagcaaa
     7681 cagggtaata gctcgtttca ttacaattat ctcttcgggt tcagctgtag gtataggtga
     7741 tgctaatcac tgcctgaatg gttccctgag tggctccgcc atttaccgtc aatgctctga
     7801 cctgtaacgg gaagtgcgct gattgtgagg aatcatccac ctgaactgtt ttggttgcgc
     7861 cagtattcaa tgtgttgcca ctgtcatcct gtagctctaa ctggatgttt tgcgcggtcc
     7921 cctggttttt ataatatccg gtgctgtcgg ctgccccgct gaagctggca gtgaccctcg
     7981 acgttcccac cggacaatta gtcaactcaa gcgcaacatc atgccaggcc gatgccgccc
     8041 cggcagacat aagactgaaa gaataaagat cgccgagatc taccgtggca ttggtggtgg
     8101 aaaccgtaca cggtttggcg acgaccttac cgttcaccgt gatggtgaca tcggctgcct
     8161 gtatcgtcgc acttgcgagc gccaacattg ccgccaatac atacccacgt ttgcaccatt
     8221 tcatgagcat ctccagttac tgatattcaa gagtgaaggt agccgtagca ttgatatgcc
     8281 ccgcagtgac aggcacctgt gtcgccatta gccgggcgta aaaattcagc gtatttggtt
     8341 tacccggcgt cagggtcgtc cacgaaatcg cagacgatgg agcattaagg ggtatttgat
     8401 tttgctgctc attcagaagc tgtattccca gtcccgcagc cgctgacacc gtattttcaa
     8461 gtgcaagcag gttggcattg tggctatctg caacgccggt aaacccaact tttacggcag
     8521 aaacggcatt accacagggt gacagcaaaa tacgaaatgg aacgacagga gtcgtcgcgc
     8581 caatgttgtt aaattgcttc gccgcgtttt ccatcagatc aacagtaaaa ttggttgatt
     8641 cagcggccac actacagccg ttatctctga catagccgcg gatagtaatc gtgctatccg
     8701 cagccaaaac gcgacttacc gccagccaca aaaaagcgca cagaagataa aaaggtttgt
     8761 ttctcatcac gcccccttaa cgacattcag ctgatagctg ggttaataac tgctgctgac
     8821 tctctggtgg cagttgataa ttggcgacac agtgagcatt ttcctcttct ccccatttca
     8881 cctgaacttt tcccgctaaa ggcattccgc tgaggtaaac ctgaccatta tccgcaacaa
     8941 tgccgctact ctggctactc tctgatgtca ccatcgcccc aaacggcagc ggcttattat
     9001 tgtgagttag cgtcatgagc agttttatcc caacgcgcgc tttaaactct gctcgcacga
     9061 tcgccccacg agtgggaaca acgttagcga ccgcgttatc taaatcgacg ttatcagcca
     9121 aggtattggt atccagcgcc actctatttt cccgatattc agtggcataa ggcagcacgg
     9181 cataaccacg ccagtcggta cgcacccccg tctggttttc gacttttgca tcttttgcgc
     9241 caggcgcttt aacaagcacc accgtatcgt ttaacggctg ccccagcgtt acgccattgg
     9301 catgagccaa taccccaccg ctgactccgt aatagagctg cttaatatca tcgctatggc
     9361 tgtaaccgat attggcattg ccgtaaccac cgcgataatt cagcgtggcg tagcctgtac
     9421 ttccgctatt accatcgcct cccccggcat agccggtttg cacgctataa ctgaggttgt
     9481 tgtcttccag caacgtaccg tatacaccag ccagattggt catccgaccg ttgagatcgt
     9541 gtgacatgct gtagctggca ctggcatgtc gccactgaga tttactgtca gaacgcagcc
     9601 agtggctgaa aggaatattg acgttaagcg ctaacatctg atcccgtcct ttttgccagg
     9661 cgtttttcgt caggctatag ctgagcgtcc agttgatatc ttcgaacgca gtatttaatc
     9721 cagcctggaa ttgctcatcg acattactcg ttccccaata agtttgatgg ctaccactca
     9781 aatacagtgt tgatgtgcgc ccgagttgct gagtaacggt gagctgtaat ttcccgcgtt
     9841 tgttataagc gaggttgtaa tagtcggtga atttcggctt aacctgaata actccatcct
     9901 gtgtttcgat gttgtagcca ttcattcgac tgtatgttgt atcagcgaaa ttaaaatatc
     9961 cgctggtcga ataacggtaa cccactaact gaatattcgt gcctgattca ttgagcgatt
    10021 tgttatagag aaaacgcacc gattgtccgt catgctgact gtcatcggga agtgtggaat
    10081 tagcctgcgt catatccaca gacagagcgc ccagtgcctc catgtttttc ccgataccga
    10141 aattaaaagc acgataacga tccgccagtt gcgttccacc atatattgtc cagccagccg
    10201 gaaggccgtg gagtaatgta ctctggaaaa agcggggttt ttcctgctgc gcatttccac
    10261 tacggtattc tcctgccgta atggaataac gagtatgccc ttcacgttgc aaaagcggga
    10321 ctgacgaata gggtacggta aaaatctgcg tgctgccgtc agcctctttg atcgttacct
    10381 gcaagtcacc actattacct gcggcataga tatcgttgat ggtaaaaggc cccggtggca
    10441 ccgtactatt ataaatgcca tatccatttt gtttaatagt gacctgtgca gtaccacgag
    10501 caataccgtg gatcaccggg gcaaatcctc tttggctatc gggtaacata ttgtcatctg
    10561 aggccaattg tgcgccgcga aagttaatac catcgaaaat atcgccctgg gtataaccat
    10621 cacccaacgt cagccgggaa cgtaaaggaa ttatgtctcg ctcaatccag gtaatgatat
    10681 gctgccattt atttttgcta cctgatgaac tgtcgctact gttataactc caggtggtat
    10741 tgtcgcgtaa acgccacgca ccaatattta acccactctg taggtttaaa tatgcataat
    10801 ggctgttacc cccaatccga
//
LOCUS       DAZSIO010000134        10720 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZSIO010000134 DAZSIO010000000
VERSION     DAZSIO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10720
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.134"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(68..1114)
                     /gene="aroH"
                     /locus_tag="ACHX5T_003119"
     CDS             complement(68..1114)
                     /gene="aroH"
                     /locus_tag="ACHX5T_003119"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR8468204.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(1271..2104)
                     /gene="ppsR"
                     /locus_tag="ACHX5T_003120"
     CDS             complement(1271..2104)
                     /gene="ppsR"
                     /locus_tag="ACHX5T_003120"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR8468205.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            2436..4814
                     /gene="ppsA"
                     /locus_tag="ACHX5T_003121"
     CDS             2436..4814
                     /gene="ppsA"
                     /locus_tag="ACHX5T_003121"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR8468206.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(4871..6517)
                     /gene="fadK"
                     /locus_tag="ACHX5T_003122"
     CDS             complement(4871..6517)
                     /gene="fadK"
                     /locus_tag="ACHX5T_003122"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFR8468207.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(6574..6867)
                     /locus_tag="ACHX5T_003123"
     CDS             complement(6574..6867)
                     /locus_tag="ACHX5T_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR8468208.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(6864..8153)
                     /locus_tag="ACHX5T_003124"
     CDS             complement(6864..8153)
                     /locus_tag="ACHX5T_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR8468209.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(8209..9147)
                     /locus_tag="ACHX5T_003125"
     CDS             complement(8209..9147)
                     /locus_tag="ACHX5T_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFR8468210.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(9167..9931)
                     /locus_tag="ACHX5T_003126"
     CDS             complement(9167..9931)
                     /locus_tag="ACHX5T_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFR8468211.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            10248..>10720
                     /locus_tag="ACHX5T_003127"
     CDS             10248..>10720
                     /locus_tag="ACHX5T_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFR8468212.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALAYTLA"
BASE COUNT         2584 a         2731 c         2782 g         2623 t
ORIGIN      
        1 gcagtaatgt gttgagaaga caacaagttg cgcagaaatg cccatcccat caggaatggg
       61 cacgcattca gaagcgggta tctaccgcaa aggcgagttt ttcgaccagg cgttcggtat
      121 cttcccagcc cagacacggg tcggtaatgg attgaccgta agtgagcggc tgaccgccga
      181 cgattttttg cgttccttcg cgcaggaaac tttccgccat aattccagca atctccgtag
      241 agccattgcg gatttgctga caaatatcct cacaaacttc taactggcga cggtgctgct
      301 tctggcagtt accgtggctg aaatccacca ccagatgttc aggtaaatca aactcgtgca
      361 gcgtatcgca ggctgcggcg atatcatcgg catgataatt cggttttttg ccgccacgca
      421 taataatgtg gccatacggg ttgccgctgg tctgatagat ggtcatctga ccatttttgt
      481 ctggcgagag gaacatatgg ctggcgcggg ctgcgcggat agcatccacg gcaatccgcg
      541 tgttgccatc ggtaccattt ttaaaaccta ccggacagga gagtgctgaa gccatttcgc
      601 ggtggatctg actttcggta gtacgagcgc caatcgcgcc ccaactgatt aaatcagcaa
      661 taaactgacc ggttaccata tcgaggaact cagtcgcggt agggacaccc agctcattta
      721 cctgtaaaag taatttgcgc gccagctcca gaccgtgatt tacccgatag ctgccgttta
      781 aatccggatc ggagattagc cctttccagc cgacaacagt tcgtggtttt tcaaaatagg
      841 tgcgcattac gatttccagc cgtgactggt actggttgcg cagcgactgc agacgggtgg
      901 cgtactccat tgcagcggtg agatcgtgga tcgagcaggg gccaataatg accaacagtc
      961 gcttatcttc accattcagt attttttcaa ttctgcggcg ggagtcggtg acatgggtgg
     1021 cgacgccagg cgttacggga taccgtagcg cgagttcggc gggcgttacc aggctctcaa
     1081 tacgcgcagt acggagttcg tcagttctgt tcattacaag tctcagaaat ttgttgcttc
     1141 tacggcagga ctaccggaaa gtgatgcgca tcacgataaa ccaatcgcag ctgatttcaa
     1201 gtgcgaggtg tgtcacacct cgccgggtgg ttagcatgat aacaaaaaaa caaaccaatg
     1261 cactagttct ctagtacatt cggcgactaa ggcccatgat atcgaggatc ttggtggcaa
     1321 tctcttctac cgaataattg gtactgttaa tccacgggat ctgattttta cggtacaagg
     1381 cttccacttc cgcgacttcc atcctgcact gacgaagcga ggcatagcga ctgttctccc
     1441 gacgttcctc gcgaatcgcc gccagacgtt ccgggtcgat agtcaggccg aacaatttat
     1501 gctgaagcgg tttgagcgac gcgggtagca ccagattatc catatcgtcg gcaataaagg
     1561 ggtagtttgc cgcgcggata ccaaattgca ttgccagata cagactggtg ggggttttac
     1621 cacagcgaga aacaccgagc aggatcacct gagcctgatc cagattgcgc aacgaaatgc
     1681 cgtcatcgtg ggcgagggtg taatcaatcg ccgcaatgcg cgcatcatat ttattgagat
     1741 tattagggtt aaggccatgg gtacgatgag caatcggcgt tggatccagt ttcatctctt
     1801 gttgtagcgg ggcaaccagc gcctgaacga tatcctggca aaagccttca ctttgcaaga
     1861 tgatggcgcg aatctccggc aacacgatgg agtagaagac cagcgggcgc acgcctgtct
     1921 ggtgataaat tgcgtcaatc tgatccttca ctgcccgtgc acggctctca ttttcgacaa
     1981 acggcagcgt gatgctgctg atagtgacgg gaaattgtga cattactgcg tgtcctaata
     2041 cctccgcagt tattgccgta ccatcagaaa tataaaaaac gtggcgatca acagcattat
     2101 ccattttgtt cttcccgtga agcagacata attctatgaa agcataaatt aaaagcgcac
     2161 agaagcgtag gacgtaatgt ctggtttata aaatgaacct tcattttatt ttttataaaa
     2221 acagcatttc atttttatgg tttcgtttat accgatggtt tatgtggaaa ttgtcgaata
     2281 gagcaggttt gcgcaacgct gggatcagtc ttaaaaagta aaaaaatata tttgcttgaa
     2341 cgattcaccg tttttttcat ccggttaaat atgcaaagat aaatgcgcag aaatgtgttt
     2401 ctcaaaccgt tcatttatca caaaaggatt gttcgatgtc caacaatggc tcgtcaccgc
     2461 tggtgctttg gtataaccaa ctcggcatga atgatgtaga cagggttggg ggcaaaaatg
     2521 cctccctggg tgaaatgatt actaatcttt ccggaatggg tgtttccgtt ccgaatggtt
     2581 tcgccacaac cgccgacgcg tttaaccagt ttctggacca aagcggcgta aaccagcgca
     2641 tttatgaact gctggataaa acggatattg acgatgttac tcagcttgcg aaagcgggcg
     2701 cgcaaatccg ccagtggatt atcgacactc ccttccagcc tgagctggaa aacgccatac
     2761 gcgaagccta tgcacagctt tccgccgatg acgaaaacgc ctcttttgcg gtacgctcct
     2821 ccgccaccgc agaagacatg ccggacgctt cctttgccgg tcagcaggaa accttcctca
     2881 acgttcaggg ttttgacgcc gttctcgtgg cagtgaaaca tgtatttgct tctctgttta
     2941 acgatcgcgc catctcttat cgtgtgcacc agggttacga tcaccgtggc gtggcgctct
     3001 ccgccggtgt tcagcggatg gtgcgctccg acctcgcatc ttctggcgtg atgttctcca
     3061 ttgataccga atccggcttt gaccaggtgg tgtttatcac ttccgcatgg ggccttgggg
     3121 aaatggtcgt gcagggtgcg gttaacccgg atgagtttta cgtgcataaa ccgacactgg
     3181 cggcgaatcg cccggctatc gtgcgccgca ccatggggtc gaaaaaaatc cgcatggttt
     3241 acgcgccgac ccaggagcac ggcaagcagg ttaaaatcga agacgtaccg caggaacagc
     3301 gtgacatctt ctcgctgacc aacgaagaag tgcaggaact ggcaaaacag gccgtacaaa
     3361 ttgagaaaca ctacggtcgc ccaatggata ttgagtgggc gaaagatggc cacaccggca
     3421 aactgttcat tgtgcaggcg cgtccggaaa ccgtgcgctc acgcggtcag gtcatggagc
     3481 gttatacgct gcattcacag ggtaagatta tcgccgaagg ccgtgctatc ggtcatcgca
     3541 tcggcgcggg tccggtgaaa gtcatccatg acatcagcga aatgaaccgc atcgaacctg
     3601 gcgacgtgct ggttactgac atgaccgacc cggactggga accgatcatg aagaaagcat
     3661 ctgccatcgt caccaaccgt ggcggtcgta cctgtcacgc ggcgatcatc gctcgtgaac
     3721 tgggcattcc ggcggtagtg ggctgtggag atgcaacaga acggatgaaa gacggtgaga
     3781 acgtcactgt ttcttgtgcc gaaggtgata ccggttacgt ctatgcggag ttgctggaat
     3841 ttagcgtgaa aagctccagc gtagaaacga tgccggatct gccgttgaaa gtgatgatga
     3901 acgtcggtaa cccggaccgt gctttcgact tcgcctgcct gccgagcgaa ggcgtgggcc
     3961 ttgcgcgtct ggaatttatc atcaaccgta tgattggcgt ccacccacgc gcactgcttg
     4021 agtttgacga tcaggaaccg cagttgcaaa acgaaatccg cgagatgatg aaaggttttg
     4081 attctccgcg tgaattttac gttggtcgtc tgactgaagg gatcgcgacg ctgggtgccg
     4141 cgttttatcc gaagcgcgtc attgtccgtc tctctgattt taaatcgaac gaatatgcca
     4201 acctggtcgg tggtgagcgt tacgagccag atgaagagaa cccgatgctc ggcttccgtg
     4261 gtgcgggccg ctatgtttcc gacagcttcc gcgactgctt cgcactggag tgtgaagcag
     4321 tgaaacgtgt gcgcaacgac atggggctga ccaacgttga gatcatgatc ccgttcgtgc
     4381 gtaccgtaga tcaggcgaaa gcggtggttg aagaactggc gcgtcagggg ctgaaacgtg
     4441 gcgagaacgg gctgaaaatc atcatgatgt gtgaaatccc gtccaacgcc ttgctggccg
     4501 agcagttcct cgaatatttc gacggcttct caattggctc aaacgatatg acgcagctgg
     4561 cgctcggtct ggaccgtgac tccggcgtgg tgtctgaatt gttcgatgag cgcaacgatg
     4621 cggtgaaagc actgctgtcg atggctatcc gtgccgcgaa gaaacagggc aaatatgtcg
     4681 ggatttgcgg tcagggtccg tccgaccacg aagactttgc cgcatggttg atggaagagg
     4741 ggatcgatag cctgtctctg aacccggaca ccgtggtgca aacctggtta agcctggctg
     4801 aactgaagaa ataaaataaa tccccggcgg cgttcagtcg ccggggttat gtgatccccg
     4861 aagatgaaac ttattcaatc tcttcacaga catcctgcgt taatcgccgt ataatatctt
     4921 ttcttaacaa aaacttttgt attttatctg aggcagtgcg cggtagtttt tcgattacca
     4981 cgatatgttc aggatattta tattttgcga cccgtttacg gctaaaaaaa gccgccatct
     5041 cttccagcga taatgaatga tgcggcgctt tcagcacgac ataagcgcat gatcgttcac
     5101 ctaaacgttc atcgggcatt gcaaccacac aggcatcgtg aattttagga tgctgcaata
     5161 aaatatcttc cacttcacgg ctgctaatat tttcgccgcc gcggacaata atatcttttt
     5221 tgcgtccggt aatttttata tagccagcct catccatacg gcagagatcg ccgctgtaat
     5281 accagccttc ttcatccagc gcacgggcgg ttaattcagg ttcatcaaaa taccccataa
     5341 acacattggg gccacgcgat gcttcttcac cttcgcaacc tggcggtaag gtcttgcgtg
     5401 cgtcatcgac cactttaatc tctacacctg cggcagcgta accatcggta tgcataaagc
     5461 gcgacaaaga attatcgaga ttcaccaccg catgcggcga actttctgtg gaaccataaa
     5521 cacttaataa tttaatgctg cgctgctggc attcgcgcgc cacttttttg gggattgtgg
     5581 taccgccgca aagaaagaag cgcagcgctg aaaggtctgc gggttgtttc tctagtaaat
     5641 tcaaaagatc atagacaaac ggcgttgcgc ctagcataca ggtgcaacgc tgctgctcaa
     5701 gcagcgcgag acacgcatca ggagtgaaaa tatctaacaa cacgctgcga gcgccaatta
     5761 agaatggtgc cgttacgcca tgcagaaagc ccgttgcgtg accaagtggc gcaggcatca
     5821 taaagacatc cagccaggtc agattcagcc gcgcgcaata agcccgctca ctggcgagaa
     5881 tattgttatg cgttagcatc acgccctttg gcagaccctc ggttccggag gtaaacagca
     5941 ccgcagctaa ttcatcgccg tgggtcgtta tcgccgtggt cagtgggata ttgtcggcga
     6001 taatctgact taatgagagg gaagaggtgg cgggagccag tttgtccacg ccgacaattt
     6061 gttgtagttg tggaagctga ttttgcagcg gcaggattaa atctaccgga cgcgtttgtt
     6121 taaacaacgt cggtgcaaag aacatttttg cctgacactt attgagcacc cacaccagtt
     6181 ctgcttcccg ccaggaaggc aacagcggca cggaaaccgc accgattttc aggcaggcaa
     6241 gatagataac ggtaaattca caccagccag gcagttgaaa tgcgatgcga tcgcctgatt
     6301 caataccctt cgctaacatc cagtttgcca gacagctcgc ggcgtgatcg agcgcgctat
     6361 aggtgtacga tgcaccatga ttatcgacca cggcaatttt gtctggcatc gcacgagcgg
     6421 tctgctgcca gtaatcggcc agtgaagcat cgccccatag cccttgctga cgatacgccg
     6481 cacgacgttg ttcgttaaac gttaatgtca ctttcatttt ggactctcga aacagaacat
     6541 caggcccgag ttgcgggcct gtgggatgca tgattagccg tagcgaaaat caataccgaa
     6601 ggtgcctgcg ggatattgcc actgttcgag aatagtgtta ccgcacagca cccgacaggt
     6661 gccgcactcc agacaaccgg cgggatcaaa atgaatgttt cctacgtcat cctgcttata
     6721 aagtccggca gggcaggctt tcattaattt acggaattca ttgatatcgg gattttccgt
     6781 cagaatgata tgcgggtggc cctcatcaac atggaattta ttgacgccta atttgatgtc
     6841 gacgttaacc gtagcgttct ggctcatagc gcggttgctc ccttaatgcc atctttcagc
     6901 aagttgatca gcccaatttt cttcgcgtgt cccatgatca ttttgcgtac cggctggttt
     6961 ggtttgccgt caatggtgaa catctcgttc atgatgtcgg ctaccattcg tgggtattgg
     7021 ctaaacaggc gcgggttttc catcagcgcc gggatcttgc gaaaatgctg catatcacgc
     7081 atgacgcagc tttgttccag ctcacgtttg tattgcgcca gactgttggc ggagaaatcc
     7141 gcgcgttctt tggcggcgat caccgttgtg gcggcagcct gagccgatgc aatggctaaa
     7201 tccatgccgc ggaccgtaaa acccaaattc aggcagaagc ctgcggcgtc accaacgatc
     7261 atcacgccct cgttaaccag ttgcggcacc attgccagac cgccttccgg caccatatgc
     7321 gcggaatatt caagcagttt gccgccgcta atcagcgggc gaatggcggg gtgttgttta
     7381 aaatcttcca gcatttgcgg cacgcttttt tgcgcatggg cgatatcacc caatccacaa
     7441 accagcccca aggatatgga atccttgttg gtatagagaa atcccccgcc catcaggccg
     7501 tcagaagggg aaccggcaaa cagccaggcg gcaccttcct cgcccgtaat attaaagcga
     7561 tcgttgatct gttctggtgt gaggccaata acctctttaa caccaacagc gtaatgatgc
     7621 ggatcggaag cgggaaccat tcccagcgag cggccaagca tcgagttaac gccatcagcc
     7681 agaatcacca cattcgcttc gagaatatca tccccagcct gcacgccagt gaccttgttt
     7741 ccttcacgaa ccagcgcgtc gacgcgaacg cctgggataa actgcgcgcc cgcctgctcg
     7801 gcttgttcca tcaaccacgg gtccagacga ttacgcaata cggtataaga tgcgtgttga
     7861 ggaacatctg gttgctcgcg gtgaaaatcg agggtaacgg cgctttcttc ggttaagaag
     7921 gagattttct cgcgtgtgac cttgcgttct accggcgctg atactgcaaa gcctggaatg
     7981 attgcttcaa gtgtgtgggc ataaagacgc ccgccggtca tgtttttaca tccggcactg
     8041 tcgccgcgtt ctatcaccag gacatccagc ccggctcgtg ccatgaccag tgcggcaacg
     8101 ctaccagcaa cgcccgcacc gaccacaatg gcatcaaatt tgtcatccga catgcgttat
     8161 gctcccttcc tgctgcgttt acagcgggag atgtggttga aaggggaatt agcggctcaa
     8221 ctggctaatc agggcaggga cgactttgta gatatcgccc accagaccgt agtcggcata
     8281 gttgaagatt ggcgcatttt tatctttatt gatggcgaca atcactttta cgccgttgcc
     8341 gccaaccata tgttggatct gcccggagat ccccagcgtc aggtagagat cggatttcag
     8401 caacacgccg gagacaccga tataacgttc acgctccatc cagttctcgc cttcggcaat
     8461 tggacgtgaa cagccgactt cagcattcag caccgccgcc agttcgtgga ccattttcag
     8521 gtcatcctgc gccgccagac cacgaccgac gccaaccaca cgttttgctt tgctcaggtc
     8581 cacgctgctt gcggctttcg cacggcgttc gcgacagaga atttcatgac gcggagcaac
     8641 ataaggtacc gtttctgtcg ggcactgatg agaggtatca ctggtgcacg gttcctgaac
     8701 accgggtgca agggtaatga tcgccagcgg gctgttgatc ttttcctgag cgaacgctaa
     8761 cccgccatac atccggtgtt cggcgcaaat gtgaccatcg acaatatcca ccgccgtggc
     8821 atcgttcacc agcgccgcat tcagttgcac acttaaccgt gctgccagag ctttaccacg
     8881 tttcgtcgcg gccaacagca acatagcggg gtgtttatct ttcagcaggg cagcaatgct
     8941 ttcggcgtaa ttttcagtgc gttgcagcgc gtcgttttgc tcaagaacat aaatacattt
     9001 tggaccataa ggcataactg cctgcgcctg gtcggtattt tgtacaatgg catacacctg
     9061 ttggccccat tgctgagcgc cgccaaacag ttcagcataa cgttcaggat tatcgctaaa
     9121 gacccagacg ctgtttaatt gactcatcgt ttcttctccg taggcttcag ttcagggctt
     9181 tcttcagatg ttccgccagc tcggcaatgg cctccggcga atcgttatcg agaatgatgt
     9241 gcttacgttc tgtttgcggc ggtacgcgaa tgccgaccag ttcagcaagt ggcgcgctct
     9301 ggctccagtc aatatcactt gcctgccact gatttaccgg ttttttaccc gcgccgagaa
     9361 tggctttcat cgaaggaatg cgtggcacgt taatatcgga ggtgacgcag agcacggctg
     9421 gaacagagag ttcaataact tcaacatcat cttcaagcgt gcgttcaatc accagtgtat
     9481 tgccctgacg ctgaatagca ctcactgcat taatcaccgg aagttgcaga atttctccga
     9541 ccagcaagcc aacctgctgg gcataaaggt cgccggaacc ttcaccaaag atcagtaaat
     9601 cgaagccgat cttttcaatt gctgccgcca gcgcttttgc ggtatcgaga ggcagtgcat
     9661 gttcaagttg cgcatcctgc accaaataca ggctgtgcgg cccgcgggat agcacgtctt
     9721 tgcgcacttt cgagttctgc aacaatgagc cgccaacggt cagcgcggct atctcatcgt
     9781 catctgttgc gagctggctt gcagcttcaa tggcattgag atcgaactgg ctgattttgg
     9841 cgtcggcatt gtcgaaattc agggtgtatt ctggagtgac aacaatgtcc tgttcttcag
     9901 gcaccagctt aaagcaggtt attattttca tgatttctcc tgtgagtcgc atacggaacc
     9961 atctccgatg gaaggtaaaa atggcctgaa aatagtgtgc gatagcggtg cggggggggg
    10021 cgacggagca agctgttttc cttgccaaca atcttgcaaa aaaaatgcga ttgctaaaac
    10081 aaaagtgcaa tttgcggcaa tatagttatt ccatgaccgt acaatcttgc attcagtgta
    10141 aataatcttg tttagctctc gctaaacgcg gttgtataac cgcaatacac gccgtatttt
    10201 atccggcata tattgttttt gcttgctcat ggaataggga ggttgtcatg tatcaacgct
    10261 gttttgataa tgccagtgaa acgctgtttg tcgccggtaa aacgccacgg ctttcacgtt
    10321 ttgcatttag cgatgatcca aaatgggagt ctggacatca cgttcatgac aatgaaaccg
    10381 agctgattta cgtcaagaaa ggggttgcaa ggtttaccat cgattcttcg ttatatgtcg
    10441 cacatgcgga tgacattgtg gtgatagaac gcggcaggct gcatgcggtg gcctctgacg
    10501 ttaacgatcc ggcaacgacg tgtacctgtg cgctgtacgg ctttcagttt cagggggctg
    10561 aggaaaatca gttactgcaa ccgcattctt gtccggtaat tgccgcgggg cagggaaaag
    10621 aagtcataaa aaccttattt aatgagctaa gtgtgatttt gccgcaaagt aaaaatagcc
    10681 aaacatcttc gttatgggat gcattagcct atacattagc
//
LOCUS       DAZSIO010000135        10688 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZSIO010000135 DAZSIO010000000
VERSION     DAZSIO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10688
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.135"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="ACHX5T_003128"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="ACHX5T_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR8468213.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="ACHX5T_003129"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="ACHX5T_003129"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFR8468214.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="ACHX5T_003130"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="ACHX5T_003130"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR8468215.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="ACHX5T_003131"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="ACHX5T_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR8468216.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="ACHX5T_003132"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="ACHX5T_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR8468217.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="ACHX5T_003133"
     CDS             4025..4615
                     /locus_tag="ACHX5T_003133"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR8468218.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="ACHX5T_003134"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="ACHX5T_003134"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR8468219.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="ACHX5T_003135"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="ACHX5T_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR8468220.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="ACHX5T_003136"
     CDS             complement(6551..6658)
                     /locus_tag="ACHX5T_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HFR8468221.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="ACHX5T_003137"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="ACHX5T_003137"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR8468222.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="ACHX5T_003138"
     CDS             7648..7875
                     /locus_tag="ACHX5T_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR8468223.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="ACHX5T_003139"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="ACHX5T_003139"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR8468224.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..>10688)
                     /locus_tag="ACHX5T_003140"
     CDS             complement(9447..>10688)
                     /locus_tag="ACHX5T_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HFR8468225.1"
                     /translation="HGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVCFMRLY
                     NELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSGGITYT
                     IQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLDNENMS
                     IQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAILVLKEH
                     EYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQSDNKIQ
                     GVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRIKAVKN
                     FLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPPELFSQ
                     AYKLKNKEMNAQLDYLNRYAT"
BASE COUNT         2522 a         2732 c         2690 g         2744 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataatatt cactttttaa aaactggcat ccgctttgcg aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct catctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 ggcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgact agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa tatccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc ccggcatatt tatctgttct
    10201 ggctggatac tcatattttc attatctaaa caatactcag aaaaaacatc atcacacgca
    10261 ttaatgtttt caatatattc cataaagcca taccaataat aacctttaaa atctgtgata
    10321 gactctgaac ctctgcccgg caaggacact ctaacaatat caacatcttg aacatattgt
    10381 atagtatatg taatacctcc tgaaacccct gaataaattt gtgtatcatt agtacgcaat
    10441 gttctcataa ataatgatag atcattatca ttctgcattt cctcaaattc tttttctagt
    10501 gattcacatt ccagacatgg agtatcctct tcatttactt tacgtctgag ttcattgtac
    10561 aaccgcataa aacacaccag tgtgttttca aaaagcgtga ttgaatatgt ttgatttttt
    10621 tcgtactcat tttttatatc gttccaggcc tgtttcaaat aattatttgc agtgcaacta
    10681 attccatg
//
LOCUS       DAZSIO010000136        10681 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZSIO010000136 DAZSIO010000000
VERSION     DAZSIO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.136"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..1819)
                     /gene="selB"
                     /locus_tag="ACHX5T_003141"
     CDS             complement(<1..1819)
                     /gene="selB"
                     /locus_tag="ACHX5T_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR8468226.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLR"
     gene            complement(1816..3207)
                     /gene="selA"
                     /locus_tag="ACHX5T_003142"
     CDS             complement(1816..3207)
                     /gene="selA"
                     /locus_tag="ACHX5T_003142"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR8468227.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3305..3913)
                     /locus_tag="ACHX5T_003143"
     CDS             complement(3305..3913)
                     /locus_tag="ACHX5T_003143"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR8468228.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(3984..5069)
                     /locus_tag="ACHX5T_003144"
     CDS             complement(3984..5069)
                     /locus_tag="ACHX5T_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR8468229.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5072..5434)
                     /locus_tag="ACHX5T_003145"
     CDS             complement(5072..5434)
                     /locus_tag="ACHX5T_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR8468230.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            5971..7884
                     /gene="mtlA"
                     /locus_tag="ACHX5T_003146"
     CDS             5971..7884
                     /gene="mtlA"
                     /locus_tag="ACHX5T_003146"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR8468231.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            7979..9127
                     /gene="mtlD"
                     /locus_tag="ACHX5T_003147"
     CDS             7979..9127
                     /gene="mtlD"
                     /locus_tag="ACHX5T_003147"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR8468232.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9127..9714
                     /gene="mtlR"
                     /locus_tag="ACHX5T_003148"
     CDS             9127..9714
                     /gene="mtlR"
                     /locus_tag="ACHX5T_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR8468233.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(9726..9935)
                     /gene="yibT"
                     /locus_tag="ACHX5T_003149"
     CDS             complement(9726..9935)
                     /gene="yibT"
                     /locus_tag="ACHX5T_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR8468234.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            10219..10581
                     /locus_tag="ACHX5T_003150"
     CDS             10219..10581
                     /locus_tag="ACHX5T_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR8468235.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         2569 a         2886 c         2753 g         2473 t
ORIGIN      
        1 cgcgcaataa atgatcattt ccacgacgac gcgtaaagcc aatgcggtca aaatattcca
       61 gaatctgaat tgccagcttt cggcctacgc ctaagcgatc gcggaaatcc gccgcgcagg
      121 ttgaaccaca ctcctgatcg agatcgcgga tcatattggc aaattcgaca atccgatcgt
      181 tacggtaata acgatctttg acgatcgcgg taattattcc ttgctgcgcc gcctggcgta
      241 gcgtcaggcg cattgcctgc tcgtcggtac ccgtctcttt tgccagatca cgcacccacc
      301 acggctcgtc accaaacagc ggctctgctt tttgccagat ggcctgctgc tcttcgctga
      361 agcccgcttt gtgatccggc agatgcagcc aaccgtgatg actgaggatg tcgccgcttt
      421 cgcgcatctt ttcaatcagc aacagtacta gcgcttcatc ttccattggc aacgccatac
      481 gtcgcagacg ttcgcgccca ggcccaggtt catcgcgatg ttgctcatga taagtcgcta
      541 atgtgtcgag aattttccgt tgccagcgag cggcaaccgg cgcattcaac aagctataac
      601 cagcctgaat atagccaggc tgttgcagca gttcgcgcat cccttcgccg ttgagctggc
      661 gcgcccaagc gaaatcagga agattaaccg cgccgcgttg caggtgaacg gctaacgctt
      721 cgctatcatt ctgtgccctg gcgagggaag ccagccattg cagatactct ggtttacgtt
      781 taccgcgacg cggcgggtta agcatcacga cgcgcgctcc ggccagcgtg ttgcgggcag
      841 agatatcgcg caataccagg cggtcgttat ctgccagcca taacggggtg tcgaagacca
      901 gttcagcaag gttatcttcc agcagtgaaa cgcgtcccgt gacgtggctg gcggcgtggt
      961 gaatatgcag cggctgccac tgggtcagcg gtgcatgggt ttgaagctcg acaatcaccc
     1021 gtgtgaacgg ctctggcggc acatcggcaa gcagccagtc gccacggtta atctgctctt
     1081 tttccgcatc acccgcgatg tcaagcgcga tacgctgccc ggcattggcg gtttctgttg
     1141 gctggttttg cgcatgcagc gcacgtacac gcatcggttt atttacacca gtcagccaga
     1201 gtgaatcgcc taccttcact tccccgctta acgccgtacc ggtgacgacc agcccggcac
     1261 cttttacggt aaatgcgcgg tcaatcgcga ggcggaaact atgttggctg gcgtgctcgc
     1321 gttccggcaa ctgaagcaga tgctcgcgca gggcatcaat tccccgacct tcggttgctg
     1381 cggtgataaa cagttttgcc tcagcaaaac cgtattcccg cagaacctcc tttacctggc
     1441 gttcaacctc atcaacacgc gcttcgtcca cgcgatcggc tttggtcagc gccactgtca
     1501 gcatcgggtt accggtcagc tgcaaaatcg ccagatgctc acgggtctgt gccatcacgc
     1561 cgtcatcgca cgccaccacc aacagcgcgt gatcgatacc accaacgccc gccagcatgt
     1621 tggaaagaaa cttttcatga ccgggaacgt cgataaaacc aggcacgcga ccatccggct
     1681 gcggccagta ggcatagccg agatcgatgg tcatgccgcg ctttttttct tccggcagac
     1741 ggtcagcatt tacgccagta atcgcctgca ataaggttgt tttgccgtgg tcaacgtgtc
     1801 cggcagtcgc aataatcatt tcaacaacat ctccaaaaac cgttgctcat cttcaaggca
     1861 gcgtaaatcc agccacaatc gtccgtcata aatacgacca atcaccggca ctggcaattc
     1921 acgccagcgg gcggctaatg actcaaggtg gctaccgcgt ccatcatggg gtgtaaacgt
     1981 taatgccgcg ctcggcaggc gatcaaccgg cagcgaacca ctgccaatct gcgaaagaca
     2041 tggcataacc tgtaccgcaa actccgcgcc gtaatgtgcg acaaggggag cctgtaaacg
     2101 ttgtgcctgg atttgaatga cctctgcgct gcgggtaagc aggcgcaggg tcggtaattt
     2161 ttcactcaga gcttcagggt gtaaataaag acgcaacgtg gcttccagcg ccgcgagggt
     2221 cattttatcc gcgcgtaatg cacgcttcag cgggtggctt tgcaggcggg cgatcatctc
     2281 ttttttacca acgataatcc cggcctgcgg cccgcctaac aacttgtcgc cggagaagct
     2341 caccagactg acgcccgccg caatcaactc ctgcggcatt ggctctttcg gcaaaccgta
     2401 ctggctaaga tcgaccagcg agccactgcc taaatcagtc accactggaa catccagctc
     2461 tttgccgagc gccaccagtt cagcttcgtc tatcgctttg gtgaacccct gaatgctgta
     2521 gttactggta tgtactttca tcaacagtgc ggtattttca ttcaccgcct gacgataatc
     2581 attcgcgtgc gtgcggttgg tggtccctac ttcgtgtagg gtgcagcctg cctgacgcat
     2641 aacatcggga atacgaaacg cgccgccaat ctccaccagt tcgccgcgag ataccaccac
     2701 ctctttcccg ctggcagtgg ccgccaacat caataacacc gccgccgcat tgttattgac
     2761 gatacaggca tcttccgccc ccgtaatacg gcacagcagc tgcgccagcg cccgatcgcg
     2821 atgtccgcgt ccggcgtcgt ccagatcgta ctcgagggtc actggcgaac gcatagcctg
     2881 cgcaacggct tccaccgcgg cttccgcctg taaagctcgc ccaaggttgg tatgcagcac
     2941 ggttcccgtc aggttgatca ccggacgcag cgcgctctgc gcctctttcg tcaaccgggc
     3001 atcgacttct tgcgcccagt tttcacacca cgcaggcagc gtctggctgc cacgaatcac
     3061 ttctcgcgct tcgtcgagca tctgacgcaa caattccacc acgcgggtgt gaccataagt
     3121 atcacgcaaa gaaaggaagg agctatcgcg caataagcga tcaatagccg gaagttgact
     3181 atagagggaa cgcgtttcgg ttgtcatagg aaacctggct gatcaaggcc ctctcacacg
     3241 gagaagggcg tttaacataa ccacggattg taacgtgaga tgggtcagga ggacatatcg
     3301 cgcatcaagc ctttggcggt tcggtgcgag caaaactttc gcggctaaac aggttttcga
     3361 ccagtttgac taagtgtggg cggtcgacac accaacctgg cgcaacgcgg cggaaattga
     3421 gatatccgac agcacaggca atggcgatag tcgccagatt gaccgtatcg gttttgagtg
     3481 tgccatcgac cagatatcct tccagcacat ccagactgcg gttgattttc tcccgctggc
     3541 gtaacaattc atcttcagac tgctgcgccg ccggacgcgc ctgttcacgc accgatacca
     3601 gcccggcatc cataatgcca tccgccagcg cctcaatttt gcgcacccgc aacgactcca
     3661 gcggatcgcg cggcaacatc gccggagcga cattcattaa ctcaatatat tcagcgatga
     3721 tcggcgaatc aaaccagcat tcgccctctt cggtcaccag caccggcact ttccctaacg
     3781 ggttaaattg cgccacgccg ttatccgcgt tatagggcag ttcattaata aattcgaaag
     3841 ttatgccctt ttctaacaac agaataaaaa gtttgcgtac aaacgggctg gtgtagctac
     3901 cgacgagttt catgccgagt cctttgtgcg aggaaaaata tcagtatggc tcaggctcac
     3961 gcaaaacaca ggtgatataa gtctcaatga tccaaataaa gataatgcat ccaactggtc
     4021 attcttagca gcactttccg catcaccgaa acatggctga aatggtcgga gtaaaccgcc
     4081 acctgggcgt agatgccgtc gggtaaggca tcgatatcat cgttagggtc cagttcaatg
     4141 gttcccagca caccgtccgt gccgggaacg accgttaatg attgcaatac cccctgcgcc
     4201 tgataagaac cgcctggcac gacaggtaaa atactagtca atttgccgtg gaacacctgc
     4261 ccaggtagcg cgttaaacac cacttccgca tcatcgccag gtttcagacg taacagcgag
     4321 ttttgccgaa attgggcgac aatttgccgt ttttgctcgg ggatgaagac catcaccgga
     4381 cgcagcggca aggcagctgc gtatgtacct gggcggatca gtacctgagt gacgtagcca
     4441 ttgcttggtg cgcgaatgac agtctgctca agattatatt ttgcttcagt aagttgcgct
     4501 cttaagctca caatctgcga ttgctcgccg ttgaccatac tgtcgagctg actctggatc
     4561 tgcgcctgct ccgccaccga gcctttcact aacgcatcct gcgcgaggaa attttgccgc
     4621 gcatcgtcga tgtcacgttc cgagaacgga ttcaccgccg cctggctgcc tttcaggtaa
     4681 cgttgataat ttttaaacag acggtcgcgc tccgctgaaa cctgggtggt gttggcctgc
     4741 gcttcagtga gctgcgcacg cagcgtcttt atattatgcg tcgccgtcat caggtcagcc
     4801 tgaagtctgt caactcgcgc ctggtaacga accgggtcga gcttaaaaag cacctcgccc
     4861 ttttgaataa gctgattatt cttgtcagtg acttcagtaa caattcccgt cacctgtggc
     4921 gtgataggga tcgctatcac tgccttttgc gcggtaaaag tgtaagggtg gttgtagttc
     4981 atcaacaaaa tcaaaccact caccagaaac acgcctccca gcgccgccgt cgccagcgcc
     5041 acgtaagtta aaacaatcaa tagatccata attagatctc cgggaacgtg gaatagtcag
     5101 tctttttctc ggcggcgagt tgatgctcca gccgggaaat tcggtcagaa agtgcggcga
     5161 tttccggttc agtcgctttc tcctgcgacg caacatgtga ctgcataccc cagccacgct
     5221 ccggttgata gagcgtcgcc cagatccaca gaaacggcca gataacatgc agagtaaaca
     5281 ggctcaccca gcccgccgta tgaatagcgt cggcatgggg gtgattgcgc tttttggcaa
     5341 tcagataagg gatgtcatgt atcgcgatga ttccataaaa aatcaccaga aagacgaaga
     5401 tcagcactcc caacgcgaaa tagtttagaa acatatctgc ctcggattca cgtttatcag
     5461 tgttgttttt tggctggcag ccagaaggga gtcaggctga tattttgaca ataatccggg
     5521 ttcgcgattc tcgccataac accaaagaat aatttttaga ggtgatgagt tgcttagtta
     5581 cataacgatt gtatgacgaa ggcataacat gctgtagatc acatcaggtg aacgccgtaa
     5641 gaaaatatct tgtgattcag atcacaaaga ttcaacaaac catcaaaaca aaaatgtgac
     5701 attactcaca tttaaatgcc atttttagcg aaaatcaccg ccttgtttct tttttataca
     5761 ggcgttttgt gatgagcgtc acgtcaatta cctctctacc ccctatattt atgtgactga
     5821 tatcacacaa aagcccgtcg actggacagt taaccgattc agtgccagat ttcgcagtat
     5881 ctacaaggtc cggctacctc tgccgccaca ttaacaaaaa acctcgggct tccagcctgc
     5941 gcgacagcaa acataagaag gggtgttttt atgtcatccg atattaagat caaagtgcaa
     6001 agctttggtc gtttcctcag caacatggtg atgccaaata tcggcgcgtt tatcgcgtgg
     6061 ggtatcatca ccgcgttatt tattccaaca gggtggttac cgaacgagac gctggcgaag
     6121 ctggtcgggc cgatgatcac ttatctccta ccgctgctga tcggttatac cggtggtaag
     6181 ctggtaggcg gcaaacgtgg tggcgtagtc ggtgccatta ccaccatggg cgttatcgtc
     6241 ggcgcagaca tgccgatgtt cctcggttct atgattgcag gtccgctggg cggctggtgc
     6301 attaagcact tcgaccgctg ggtagacggt aagatcaaat ccggttttga gatgctggtg
     6361 aataacttct ccgcaggcat catcgggatg atcctcgcta ttctggcatt cctcggcatt
     6421 ggcccgattg ttgaagccct gtccaaaatg ctggctgcgg gcgttaactt catggttgtc
     6481 catgacatgc tgccgctggc gtctatcttt gttgaaccgg cgaaaatcct gttcctcaac
     6541 aacgccatta accacggtat cttctcgccg ctgggtattc agcagtccca tgaactgggt
     6601 aaatcaatct tcttcctgat tgaagctaac ccaggtccag gtatgggcgt gctgctggcg
     6661 tacatgttct ttggtcgtgg tagcgctaaa cagtctgcgg gcggtgcggc aatcatccac
     6721 ttcctgggtg gtatccacga aatctacttc ccgtatgtgc tgatgaatcc gcgtctgatc
     6781 ctcgcagtca tcctcggcgg tatgactggc gtgttcacgc tgactatcct gggcggtggt
     6841 ctggtttctc cggcatctcc gggttctatc cttgctgtac tggcgatgac accaaaaggt
     6901 gcttatttcg ctaacatcgc gggtgtgtgt gcggcgatgg ctgtctcctt cgttgtctct
     6961 gctattttgc tgaaaaccag caaagtgaaa gaagaagatg atattgaagc agcaactcgt
     7021 cgtatgcagg acatgaaagc tgagtctaaa ggcacatctc cgctatctgc gggcgatgtg
     7081 actaacgacc tgagccacgt acgtaaaatc atcgttgcct gtgacgccgg tatgggttca
     7141 agtgcgatgg gcgcaggcgt gctgcgtaag aaaattcagg atgcaggtct gtcgcagatt
     7201 tcggtcacta acagcgcgat caacaacctg ccgccagatg tggacctcgt catcactcac
     7261 cgtgacctga ccgaacgcgc tatgcgccag gttccgcagg cacagcatat ttcgctgacc
     7321 aacttcctcg acagcggcct gtacaccagc ctaaccgaac gtctcgttgc tgcccagcgc
     7381 catactgaaa acgaagtgaa agtgaaagac agtctgaaag acagctttga cgattccagt
     7441 gctaacctgt tcaaactggg cgcggagaac atcttcctcg gtcgcaaagc agcaaccaaa
     7501 gaagaagcga ttcgttttgc tggcgagcag ctggtgaaag gcggttacgt tgagccggaa
     7561 tacgttcagg cgatgctgga tcgtgaaaaa ctgaccccga cttatctggg tgagtctatc
     7621 gcggtgccac acggtacggt tgaagcgaaa gatcgcgtac tgaaaacggg cgtggtgttc
     7681 tgccagtacc cggaaggcgt gcgcttcggt gaagaagaag atgacattgc ccgtctggtg
     7741 attggtattg cagcccgtaa caacgagcac attcaggtta tcaccagcct gaccaatgca
     7801 ctggatgatg agtccgtcat cgagcgtctg gcacacacca ccagcgtgga tgaagtgctg
     7861 gaactgctgg caggtcgtaa gtaatccaat cctaccctct cctcacggag agggtttggg
     7921 tgagggaaaa gcctcacccc agccctctcg ggtaaaaaca ttgatgaagg ttaatactat
     7981 gaaagcatta cattttggcg caggtaatat cggtcgtggc tttatcggta aactgctggc
     8041 agacgcgggt atccaactga cgtttgccga tgtcaatcag gtggtacttg atgccctgaa
     8101 tgcccgtcat agctatcagg ttcatgtggt cggtgaaacc gagcaggtag ataccgtttc
     8161 cggcgtcaat gctgtcagca gcattggtga tgatgtcgtt gatctgattg ctcaggtcga
     8221 tttagtcact accgccgttg gcccggttgt gctggaacgt attgctccgg ctatcgccaa
     8281 agggctggtg aaacgcaaag aacaaggtaa tgaatccccg ctgaacatca tcgcctgtga
     8341 aaacatggta cgcggcacca cgcagctgaa aggtcatgtg atgaacgccc tgccggaaga
     8401 cgccaaagcg tgggtagaag agcacgttgg ctttgtcgat tccgccgttg accgcatcgt
     8461 accgccttcg gcttcggcaa ctaacgatcc gctggaagtg acggtagaaa ctttcagcga
     8521 atggattgtc gataaaacgc agttcaaagg cgcactgccg aacatcccag gcatggagtt
     8581 aaccgacaac ctgatggcat ttgtcgaacg taaactcttc accctgaaca cgggtcatgc
     8641 tataaccgcg tacctcggaa aactggccgg tcatcagacc attcgtgacg ctattctcga
     8701 cgagaaaatc cgcgcggtgg taaaaggtgc gatggaagaa agtggtgcgg tactgatcaa
     8761 gcgctacggc tttgacgcag acaaacatgc ggcgtacatc cagaaaatcc tcggtcgttt
     8821 tgagaacccg tatctgaaag atgatgtaga gcgcgtaggc cgtcagccgc tgcgtaaact
     8881 gagtgctggc gaccgtctga tcaagccact gctcggtacg ctggaataca gccttccgca
     8941 caagaatctg attcagggga ttgctggtgc aatgcacttc cgcagtgaag atgatccaca
     9001 ggctcaggaa ctggcagcac tgatcgctga caaaggtccg caggcggcgc tggcacagat
     9061 ttccggtctt gatgccaaca gcgaggttgt atccgaggcg gtaaccgctt ataaagcaat
     9121 gcaataatgg tggaccaggc gcaggacacc ctgcgcccga ataacagatt gtcagatatg
     9181 caggcaacaa tggaacaaac ccaggccgtt gaaaaccgtg tgcttgagcg tctgaatgct
     9241 ggcaaaaccg tgcgaagctt tctgatcacc gccgtcgagc ttttgaccga ggcggtaaat
     9301 cttctggtgc ttcaggtatt ccgcaaagac gattacgcgg tgaagtatgc tgtagaaccg
     9361 ttactcgacg gcgacggtcc gctgggcgat ctttctgtgc gtttaaaact catttacggg
     9421 ttgggcgtca ttaaccgcca ggaatacgaa gatgcggaac tgctgatggc attgcgtgaa
     9481 gagctaaatc acgacggcaa cgagtacgcc tttaccgacg acgaaatcct tggacccttt
     9541 ggtgaactgc actgcgtggc ggcgttacca ccgccgccac agtttgaacc agcagactcc
     9601 agtttgtatg caatgcaaat tcagcgctat caacaggctg tgcgatcaac aatggtcctt
     9661 tcactgactg agctgatttc caaaatcagc ttaaaaaaag cctttcaaaa gtaagcaacg
     9721 tctgcttact gcccctctac ctgcttcggc cgataaagcc gacgataata ctccagacgt
     9781 tgtagatata acggcacgct ttcatcgggt attccggacg gaatcgcgtt acggggagga
     9841 agttttttca gatactcccg gaacgcctgg cttgatgcca tgaaatctac ggctttatcg
     9901 ataagaagcg gaacgttttc acctaatttg cccatgatga tatctccgta ttaccccgcg
     9961 ccgggaatgc gcggccgcca attttagttt aggttcccgg taaagatgac attaaggaaa
    10021 acgtgctgaa tcctcaataa tcatccgctg ctgtttatta tctttttcta tagatttctt
    10081 aattaatcaa cgaattgcgt agtttttgag catgttaatg attacgttgc agaactatta
    10141 caatgtcatc agttgtgaac aaagcacctg gtcgcgcata ctaggggcta taaatttatc
    10201 tttatcagaa gccatcacat gaaagaagtc gaaaaaaacg aaatcaagcg tctcagcgat
    10261 cgcctggacg ccatccgcca ccagcaggcc gatctgtcgc tggttgaagc cgcagacaaa
    10321 tatgccgagc tggaaaaaga gaaagccacg ctggaagcag aaattgctcg cctgcgtgag
    10381 gttcatagcc aaaaactgag taaagaagca cagaagctga tgaagatgcc gttccagcgc
    10441 gcaattacca aaaaagagca ggctgatatg ggcaagctga agaaaagtgt tcgcggactg
    10501 gtggttgtgc acccaatgac cgcgctgggg cgcgaaatgg gcctggaaga gatgactgga
    10561 ttttctaaga ctacgtttta agcccctcag acgactggtt cgatcgtatc aacccgcatc
    10621 tggcttgcct tagctggtca atgcggggac tctcctgtct actcgcctaa ttaccaaaca
    10681 a
//
LOCUS       DAZSIO010000137        10509 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZSIO010000137 DAZSIO010000000
VERSION     DAZSIO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.137"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..2091
                     /locus_tag="ACHX5T_003151"
     CDS             <1..2091
                     /locus_tag="ACHX5T_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR8468236.1"
                     /translation="LKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSS
                     ITAPDRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAIN
                     IPDYELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKK
                     PGEAILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAY
                     QPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADI
                     RAKISKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKD
                     EWKNLHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQI
                     ERSITPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLS
                     AIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDF
                     LFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCS
                     TYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGN
                     EEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSAL
                     LLGKTGEPPNYLYL"
     gene            2427..3359
                     /gene="glaH"
                     /locus_tag="ACHX5T_003152"
     CDS             2427..3359
                     /gene="glaH"
                     /locus_tag="ACHX5T_003152"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR8468237.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3379..4647
                     /gene="lhgO"
                     /locus_tag="ACHX5T_003153"
     CDS             3379..4647
                     /gene="lhgO"
                     /locus_tag="ACHX5T_003153"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR8468238.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4670..6118
                     /gene="gabD"
                     /locus_tag="ACHX5T_003154"
                     /pseudo
     CDS             4670..6118
                     /gene="gabD"
                     /locus_tag="ACHX5T_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            6126..7412
                     /gene="gabT"
                     /locus_tag="ACHX5T_003155"
                     /pseudo
     CDS             6126..7412
                     /gene="gabT"
                     /locus_tag="ACHX5T_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            7650..9050
                     /gene="gabP"
                     /locus_tag="ACHX5T_003156"
     CDS             7650..9050
                     /gene="gabP"
                     /locus_tag="ACHX5T_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR8468239.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9071..9733
                     /gene="csiR"
                     /locus_tag="ACHX5T_003157"
     CDS             9071..9733
                     /gene="csiR"
                     /locus_tag="ACHX5T_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR8468240.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9734..10183)
                     /gene="kbp"
                     /locus_tag="ACHX5T_003158"
     CDS             complement(9734..10183)
                     /gene="kbp"
                     /locus_tag="ACHX5T_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR8468241.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10267..10425)
                     /locus_tag="ACHX5T_003159"
     CDS             complement(10267..10425)
                     /locus_tag="ACHX5T_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR8468242.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2641 a         2617 c         2723 g         2528 t
ORIGIN      
        1 ttaaaagaca gagaaattat atcaactttt cacgcagaaa aagtcattaa tgatctgtta
       61 aaaaacgatt ataaaatttc cctttctcca gatacaactt accgagagct gaataaagct
      121 gcacagagta gcattacagc gccagacagg ataggagaag ggaaaacatg ggtttatcaa
      181 cgagatacaa tggttgaaag aggtgataac agtggtgttc atcagtatgg tccagctgaa
      241 catttcactc acattatatc tgacaaaccg tccccaaaag ataagtatgt tgcatatgct
      301 attaacattc ctgactatga gctagcagcc gatgtatata atattaacgt gacgtcacct
      361 tccggacagc aagaaacatt taaaatacta atcaatccag aacatctacg gcaaacactt
      421 gagcgtaaat ctcttactgc tgttcagaaa tcacaatgtg aaatcatcac ccccaaaaaa
      481 cctggcgaag cgattcttca tgcttttaat gccacctacc agcaaatcag ggaaaatatg
      541 tctgagtttg cacgttccca ttatgggtat atacaaatcc ctccagtgac aactttccgc
      601 gccgacggac cagaaactcc cgaagaagaa aaaggttact ggtttcacgc ttatcaaccc
      661 gaagatcttt gtaccatcca taatccaatg ggggatttgc aggattttat cgcattggtt
      721 aaagatgcta aaaaatttgg tatcgatatc attcctgatt atacctttaa ctttatggga
      781 atcgggggaa gtggtaaaaa tgacctggat tatccctctg ctgatatacg agcgaagatc
      841 agtaaagata tagaaagtgg tatccctggc tattggcaag gtcaggtttt gattccattc
      901 attaaagatc cagtaacaaa agaacgtaaa caaatccatc cagaagatat acatctcact
      961 gcaaaagact tcgaagcaag taaagataac atctctaagg atgaatggaa aaacctccat
     1021 gcattaaaag aaaagagttt aaatggaatg cctaaaacaa cccccaaaag tgaccaggtt
     1081 attatgttgc aaaatcaata cgttcgtgaa atgcgaaaat atggcgtacg aggtttacgt
     1141 tatgatgcag caaaacactc aaaacatgaa caaatagaaa gatcaataac cccaccgctt
     1201 aaaaattata atgagcgatt acacaatact aacttattta acccaaaata tcatgaaaaa
     1261 accgttatga attacatgga atatctggta acttgtcagt tgaatgaaga acaaatgtca
     1321 tcgctgcttt atgaaagaga tgatttaagc gccattgatt tttcattgct catgaagacg
     1381 ataaaagcct tttcattcgg gggagatctc ctaacccttg catcaaaacc gggttccaca
     1441 atctcaagca tcccatcaga aagacggata ttgattaaca ttaaccacga ttttcctaac
     1501 aatggcaatc ttttcaatga ctttctattt aaccatcaac aagatgaaca attagcaatg
     1561 gcatatatgg ccgctctccc gttcagcagg cctttagttt actgggatgg ccaggtatta
     1621 aaatcaacga ctgaaattaa aaattatgat gggcccacgc gtgtcggtgg tgaggcgtgg
     1681 cttaataaag gttgctctac ctatcagcag ctctacaatg aattccacgc attatatata
     1741 gataaagcag gaatatggag cgcatttgag ggtgtatttg caactaaaaa cgttctggcc
     1801 tttagtcgtg gggattctgt gaacattaat cactctcctc atgatggact agttataata
     1861 aataaaggaa acgaagaagt tgaaggtacc tggcctaaca aattgcaacc tggaatatat
     1921 aaaaacatgg ggagtaatag cgttaacatt attattaata atacccgaaa aattatcccc
     1981 ccttgtaaag tatttacgct tagaggcgga tctctaaata tcaatattcc taggcgtagc
     2041 gctcttcttt tagggaaaac aggagaaccg ccgaactatc tctatttata atttaatgtt
     2101 atatctgccc cgataaaacg gggcagataa tatgtttagt ttactaacgg tcattttgta
     2161 gtgaagccat ttactgtttt ttatcgacca gataatctgt tctctaatgt taactccccc
     2221 taacctgttg ctttagttat tcatttcctg tctcactttg ccttaatacc ctacgttaaa
     2281 tgttactaat ttgttgcttt tgatcacaat aagaaagcaa tatgtcgctt ttgtgcgcat
     2341 ttttcagaaa tgtagatatt tttagattat ggctacgaaa tgagcatcgc catgtcaccc
     2401 tacatctcat aagaggatcg cttctgatga atgcactgac cgccgtacaa aataacgctg
     2461 tcgattcagg ccaggactat agcggattca ctctcatccc gtcggcgcaa tccccgcgtc
     2521 tgctggaact caccttcacc gaacagacga ccaaacagtt tctcgaacag gttgccgaat
     2581 ggcccgtgca ggcgctggag tacaaatcgt ttctgcgttt tcgggtaggc aaaattcttg
     2641 acgatctgtg tgcgaatcag ctgcaaccat tgctgttgaa gaccctgcta aaccgtgctg
     2701 aaggtgcgct gttgatcaat gcggtaggta tcgatgatgt ggcgcaggca gatgagatgg
     2761 tgaagctggc aacggcggta gcgcatctga ttggtcgctc aaatttcgat gcgatgagcg
     2821 gtcagtatta cgcgcgtttc gtggtgaaaa atgtcgataa ctcagacagc tatctgcgtc
     2881 agccgcaccg cgtgatggaa ctgcacaacg acggcacgta tgtcgaagag atcaccgatt
     2941 acgtgctgat gatgaaaatc gatgactggg aacatctgga ccactatttc cgccatccac
     3001 tggcgcgtcg cccgatgcgc tttgccgcgc cgccgagcaa aaacgtcagc aaagatgttt
     3061 tccatccagt gttcgacgtt gatcaacagg gtcgcccggt gatgcgctat atcgaccagt
     3121 tcgtccagcc aaaagatttt gaagaaggcg tgtggttgag cgagctttcc gatgccattg
     3181 aaaccagcaa aggcattctt tctgtacccg ttcccgttgg caaattcctg ttgataaaca
     3241 acctgttctg gctgcacggt cgcgaccgct ttactccgca cccggatctg cgccgtgaac
     3301 tgatgcgtca gcgtggctat ttcgcttacg ccactcacca ctaccagacg catcagtaag
     3361 cgcaaaggaa ttgagcggat gtatgatttt gtgattattg gcggcggcat catcggcatg
     3421 tcgaccgcca tgcaactgat tgatgtttat ccggatgccc gcattgcgtt gctggaaaaa
     3481 gagtccggcc cggcctgtca ccagacgggc cacaacagcg gcgtgatcca tgccggggtc
     3541 tattacacgc ccggcagcct gaaggcgcag ttttgcctgg cggggaaccg tgccaccaaa
     3601 gccttttgcg atcaaaacgg cattcgctat gacaactgcg gcaagatgct ggtcgccacg
     3661 tctcagctcg aaatggaacg gatgcgcgcc ttatgggaac gcacagcggc gaacggtatc
     3721 gagcgcgagt ggataaacgc cgtggaactg cgcgagcgcg aaccgaatat caccgggctt
     3781 ggtggcattt ttgtgccgtc cagcggcatt gtcagctacc gcgaagtgac ggcggcaatg
     3841 gcaaaaatct tccaggccag aggcggcgag attatttata acgccgaagt cagcgccctc
     3901 agtgagcata aaaacggcgt ggtgatacgc acccgtcagg gcggtgaata tgaagcatcg
     3961 acgctgatta gctgttccgg gctgatggct gaccggctgg tgaaaatgct cggcctcgaa
     4021 ccgggcttta ttatctgccc gttccgtggc gagtatttcc gccttgcgcc ggagcataac
     4081 cagattgtta accacctgat ttaccccatt cccgaccccg cgatgccatt tttgggcgtg
     4141 catctcaccc gcatgatcga cggcagcgtg accgtcgggc caaacgcggt gctggctttc
     4201 aaacgcgaag gctatcgcaa gcgcgacttc tcatttagtg acacgctgga gattttgagc
     4261 tcgtcgggga ttcgccgggt gctgcaaaac catctacgct caggactggg cgagatgaaa
     4321 aactcgctgt gcaaaagcgg ctatctgcgg ctggtgcaaa agtattgtcc ccggctttcg
     4381 ttaagcgatc tccagccctg gcccgccggt gtgcgggcgc aggcagtatc gccggacggc
     4441 aagctgattg acgattttct gtttgtcacc accccacgca cgatccacac ctgcaatgcg
     4501 ccctccccgg cagcgacatc agcaattcct attggtgcgc atattgtcag caaggtacaa
     4561 acgctgttgg caagccagag taaccccgga cgcacgctgc gagcggcacg tagtgtggat
     4621 gccttacacg ccgcatttaa tcaataacct ttgaaaacag gacgtaacaa tgaaacttaa
     4681 cgacagtaac ttattccgcc agcaggcgtt gattaacggg gaatggctgg acgccaacaa
     4741 tggtgaagtc atcgacgtca ccaatccggc gaacggcgac aagctgggta gcgtgccgaa
     4801 aatgggcgca gatgaaaccc gcgccgctat cgacgccgcc aaccgcgccc tgcccgcctg
     4861 gcgtgcgctc accgccaaag aacgcgccac cattctgcgc aactggttca atttgatgat
     4921 gcagcatcag gacgatttag cgcgcctgat gaccctcgaa cagggtaaac cgctggctga
     4981 agcgaaaggc gaaatcagct acgccgcctc cttcattgag tggtttgctg aagaaggcaa
     5041 acgtatttat ggcgacacca ttcctggtca tcaggccgat aagcgcctga ttgttatcaa
     5101 gcagccgatt ggcgtcaccg ccgccatcac gccgtggaac tttccggcgg cgatgattac
     5161 ccgtaaagcc ggtccggcgc tggcggcagg ctgcaccatg gtgctgaagc ccgccagtca
     5221 gacgccgttc tctgcgctgg cgctggcgga actggcgatt cgcgcgggca ttccggctgg
     5281 cgtgtttaac gtggtcaccg gttcggcagg cgtggtcggt aatgaactga ccagcaaccc
     5341 gctggtacgc aaactgtcgt ttaccggtta gaccgagatt ggccgccagt taatggaaca
     5401 gtgcgcgaaa gacatcaaaa aagtgtcgct ggagctgggc ggcaacgcgc cgtttatcgt
     5461 ctttgacgat gccgatctcg ataaagccgt ggaaggcgcg ctggcctcga aattccgcaa
     5521 cgccgggcaa acctgcgtct gcgccaaccg tttatacgtg caggacggcg tgtatgaccg
     5581 ctttgccgaa aaattacagc aggcggggag caaactgcac atcggcgacg ggctggagaa
     5641 aggcgtcacc atcgggccgc tgatcgatga aaaagcggta gcaaaagtgg aagagcatat
     5701 tgccgatgcg ctggagaaag gtgcgcgtgt ggtttgcggc ggtaaagcgc acgaacgcgg
     5761 cggcaacttc ttccagccaa ccattctggt ggacgttccg gccaacgcca aagtgtcgaa
     5821 agaagagacg ttcggccccc tcgccccact gttccgtttt aaagatgaag ccgatgtgat
     5881 tgcacaagcc aatgacaccg agtttggtct tgccgcctat ttctacgccc gtgatttaag
     5941 ccgcgtcttc cgcgtcggcg aggcgctgga gtacggcatc gtcggcatca ataccggcat
     6001 tatttccaat gaagtggccc cattcggcgg catcaaagcc tcgggtctgg gtcgtgaagg
     6061 ttcgaagtat ggcatcgaag attacttaga aatcaaatat atgtgcatcg gtctttaact
     6121 ggagaatgcg aaggaacagc aataaagagt taatgcagcg ccgcagtcag gcgattcccc
     6181 gtggcgttgg gcaaattcac ccgattttcg ctgaccgcgc ggaaaactgc cgggtgtggg
     6241 acgtttaagg ccgtgagtat cttgatttcg cgggcgggat tgcggtgctc aataccgggc
     6301 acctgcatcc gaaggtggtg gccgcggtgg aagcgcagtt gaaaaaactg tcgcacacct
     6361 gcttccaggt gctggcttac gagccgtatc tggagctgtg cgagattatg aatcagaagg
     6421 tgccgggcga tttcgccaag aaaacgctgc tggttacgac cggttccgaa gcggtggaaa
     6481 acgcggtaaa aatcgcccgc gccgccacca aacgtagcgg caccatcgct tttagcggcg
     6541 agtatcacgg gcgcacgcat tacacgctgg cgctgaccgg caaggtgaat ccgtactctg
     6601 cgggcatggg gctgatgccg ggtcatgttt atcgcgcgct ttatccttgc ccgctgcacg
     6661 gcataagcga ggatgacgct atcgccagca tccaccggat cttcaaaaat gatgccgcgc
     6721 cggaagatat cgccgccatc atgattgagc cggttcaggg cgaaggcggt ttctacgccg
     6781 cgtcgccagc ctttatgcag cgtttacgcg ctctgtgtga cgagcacggg atcatgctga
     6841 ttgccgatga agtgcagagc ggcgcggggc gtaccggcac gctgtttgcg atggagcaga
     6901 tgggcgtggc gccggatctt accacctttg cgaaatcgat cgcaggcggt ttcccgctgg
     6961 cgggcgtcac cgggcgcgcg gaagtgatgg acgctgttgc accgggcggt ctgggcggca
     7021 cctatgccgg taacccgatt gcctgcgtgg ctgcgctgga agtgttgaag gtgttcgagc
     7081 aggaaaatct gctgcaaaaa gccaacgatc tggggcagaa gctaaaagac ggattgttgg
     7141 cgatagccga aaaacacccg gagatcggcg acgtacgcgg gctgggatcg atgattgcca
     7201 tcgagctgtt tgaagatggc gatcacagca aaccggacgc aaaactcacc gccgagatcg
     7261 tggcacgcgc ccgcgataag ggcctgattc ttctctcctg cggcccgtat tacaacgtgc
     7321 tgcgcatcct tgtaccactc accattgaag acgctcagat ccgtcagggt ctggagatta
     7381 tcggtcagtg ttttgatgag gcgaagcagt agcaccgtcc ccatgcagga ggcgacgctg
     7441 cgcgtcttgt ccggcctacg agaatccaag tcggataagg cgtttacgcc gcatccgaca
     7501 atctgtacgt gaacaggaat aaatctatgt cggccggata aggcggagcc gctctccggc
     7561 aaaaagaatc aataacaatt aaacgcgtga cccgacgcgg gaaatgtcgg ggcgctctcc
     7621 caagtgacac actttcgaga ggattcagga tggggcaatc atcgcaacca cacgagttag
     7681 gcggcgggct gaaatcacgc cacgtcacca tgttgtctat tgccggtgtt atcggcgcaa
     7741 gtctgtttgt cggttccagc gtcgccatcg ccgaagcgga cccggcggta ttactggcct
     7801 atctgttcgc cggattactg gtggttatga ttatgcggat gctggcggaa atggcagttg
     7861 ccacgcccga taccggttcg ttttccacct atgccgataa agccattggc cgctgggcgg
     7921 gctataccat cggctggttg tactggtggt tttgggtact ggttatccca ctggaagcca
     7981 acatcgccgc catgatcctg cactcgtggg ttccaggcat tcccatctgg ttattttccc
     8041 tcgtcattac cctcgcctta actggcagta atttattaag cgttaaaaac tacggcgaat
     8101 ttgagttctg gctggcgctg tgcaaagtca tcgctatcct ggcctttatt ttccttggtg
     8161 cagtcgcaat tagcggtttt tacccttatg ccgaagtgag cgggatctca agattgtggg
     8221 atagcggcgg ctttatgccc aacggtttcg gtgcggtatt aagcgcgatg ttgatcacca
     8281 tgttctcgtt tatgggcgca gaaattgtca ccattgccgc cgcggaatcc gacatgccgg
     8341 aaaaacatat tgtccgcgcc accaactcgg ttatctggcg tatttctatc ttctatttgt
     8401 gctctatttt tgtcgtcgtg gcattaatac cgtggaatat gccggggctg aaagccgttg
     8461 gatcttatcg ctcggttctg gaattgctca atattcccca tgcgaaatta atcatggact
     8521 gtgtgatatt actttccgta accagttgcc tgaactcggc cctgtatacc gcctcaagga
     8581 tgctctactc cttaagccgt cgcggtgatg ctcccgcggt aatgggtaaa atcaaccgca
     8641 gtaaaacccc gtacgtggcg gtgttactct ccaccggcgc agcattctta acggtggtgg
     8701 tgaactatta cgcgcctgcg aaggtattta aatttctgat cgacagctcc ggcgctatcg
     8761 ccctgctggt ttatttagtc atcgccgttt cacagttgcg gatgcgcaaa attctgcgag
     8821 cagaaggaag cgaaattcgc ttgcgaatgt ggcttgatcc ctggctcacc tggctggtca
     8881 tcggctttat tacctttgtg ttggtagtga tgctattccg tccggcgcaa cagttagaag
     8941 tgatctccac cggcttatta gcgataggga ttatctgtac cgtgccaatt atggctcgct
     9001 ggaaaaagct ggtattgtgg caaaaaacac ccgttcataa tacgcgctga taatgatcag
     9061 gagtcacacc atgaccatta cgtctctgga tggctatcgc tggctgaaga acgatattat
     9121 tcgcggtaat tttcaaccgg atgaaaaatt acggatgagt ttactgacat cgcgttatgc
     9181 acttggcgtt gggccgttgc aggaagcact ttctcaactg gtggcggaac ggctggtcac
     9241 ggtggtgaat caaaaagggt atcgggtggc gtctatgtca gagcaggagc tgctcgatat
     9301 tttcgacgcc cgcgccaata tggaagcgat gctggtcagt ctggcgattg cccgcggtgg
     9361 cgatgaatgg gaggcagacg ttctcgcaaa agcgcatctg ctcagtaagc tggaagcctg
     9421 tgacgccagc gagaaaatgc ttgatgagtg ggatctgcgt catcaggcgt ttcatacagc
     9481 aattgtggcg ggctgtggtt ctcactattt gctgcaaatg cgtgaacggt tgtttgatct
     9541 ggcggcgcgt tatcgattta tctggctgcg gcgaacggtg ctttcggtgg aaatgctgga
     9601 ggataaacac gatcagcacc agaccctgac tgcggcggta ctgccgcgag atacagcgcg
     9661 cgccagtgag ttaatgcgcc agcatttact gacgccaatt cccattatcc agcaggcgat
     9721 ggctggcaat taactactct tccggaatac gcaacacttg ccccggataa attttatccg
     9781 ggctttttag catcggttta ttcgcttcga agattttatt gtacagatta gcgttaccgt
     9841 agacctgttt ggaaatggca ctcagagtgt cgccagactt aacggtataa aactggctgg
     9901 cagtggctgg tgtcgccgtt ttcacctgat catcaacact ggcaataccg gaaatattcc
     9961 ccaccgcaac aaggattttc tccttcgcct cctgactcag gccgtcacca gtgaccgtcg
    10021 ctttgccgtc ggcaatttga atgttcactt tatcggcatc aggtataccg gttttgctca
    10081 gatgctcctg caccttcttc gcctgatcgt ctttatcgtg ctgacctgta accgcatccc
    10141 agagtttttc tccggcatct ttaacaaaat tgaacagacc catagctacc tcattcgtta
    10201 acggaaacac tcagaaagtg tagcagagga acgcggcgcg ttaacgccgc gggggtgagc
    10261 acaggtttaa tcgcgggttt gcacccagaa cgcgtgaatc aaaccgggga tatacccaag
    10321 cagtgtcagc agaatattaa taatgaacgc ccaaccgaac cctttaccga gcagcacgcc
    10381 gatcggcggc agaatgatgg tgatgacgat tctccagaaa cccatatgta ctccctataa
    10441 gaaaattact cattgtttaa aaagagattt tatctcttaa gcgtagtaat tttagcggag
    10501 cctgccagt
//
LOCUS       DAZSIO010000138        10464 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZSIO010000138 DAZSIO010000000
VERSION     DAZSIO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.138"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            2..202
                     /gene="cspG"
                     /locus_tag="ACHX5T_003160"
     CDS             2..202
                     /gene="cspG"
                     /locus_tag="ACHX5T_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR8468243.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            213..401
                     /locus_tag="ACHX5T_003161"
     CDS             213..401
                     /locus_tag="ACHX5T_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR8468244.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            376..606
                     /gene="ymcE"
                     /locus_tag="ACHX5T_003162"
     CDS             376..606
                     /gene="ymcE"
                     /locus_tag="ACHX5T_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR8468245.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            596..769
                     /locus_tag="ACHX5T_003163"
     CDS             596..769
                     /locus_tag="ACHX5T_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR8468246.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(818..1891)
                     /locus_tag="ACHX5T_003164"
     CDS             complement(818..1891)
                     /locus_tag="ACHX5T_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFR8468247.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1993..4704)
                     /gene="torS"
                     /locus_tag="ACHX5T_003165"
                     /pseudo
     CDS             complement(1993..4704)
                     /gene="torS"
                     /locus_tag="ACHX5T_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4787..5815
                     /gene="torT"
                     /locus_tag="ACHX5T_003166"
     CDS             4787..5815
                     /gene="torT"
                     /locus_tag="ACHX5T_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR8468248.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5788..6480)
                     /gene="torR"
                     /locus_tag="ACHX5T_003167"
     CDS             complement(5788..6480)
                     /gene="torR"
                     /locus_tag="ACHX5T_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR8468249.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHELSADLLVTQ
                     HGEGYFLAADVC"
     gene            6567..7739
                     /gene="torC"
                     /locus_tag="ACHX5T_003168"
     CDS             6567..7739
                     /gene="torC"
                     /locus_tag="ACHX5T_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR8468250.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7739..10279
                     /gene="torA"
                     /locus_tag="ACHX5T_003169"
                     /pseudo
     CDS             7739..10279
                     /gene="torA"
                     /locus_tag="ACHX5T_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10276..>10464
                     /gene="torD"
                     /locus_tag="ACHX5T_003170"
     CDS             10276..>10464
                     /gene="torD"
                     /locus_tag="ACHX5T_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR8468251.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2523 a         2686 c         2789 g         2466 t
ORIGIN      
        1 aatgactggt ttagtaaaat ggtttaacgc agataaaggt tttggcttta tcactcctga
       61 tgatggcagc aaagacgttt tcgtccattt caccgccatc cagagcaatg aattccgcac
      121 gctgaacgaa aatcagaaag ttgaattttc tattgagcag gggcaacgtg gccccgcggc
      181 agcgaacgtt gttacgctct aaggttgccg ttattactca acatctccat ttccgctgtc
      241 catgttgtca tggttcacag taccgtacat cggcattcga tgtgacggag cgaaaccctt
      301 tgggcgctaa gtgtattttt tgtaaatcga ctatgatcac ctttgataac gtcgcgctgc
      361 aaatacgcac tgaccatgcg ccgctggatt tcacaaaata atatcaggct ccctcgtgga
      421 gcctttttta tatctgcctt atttttcttc aacgctgtat gtatagtaag cgataacctg
      481 ttgattattg aatctttcgg gaagatggct tataacattt cttacctgac cagggtaccg
      541 ggaaccaaca ccttactggc gtgttgctgt ctttcaagac cagacgaggt taacagtgaa
      601 tattgaagag ttaaaaaaac aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat
      661 cgccgagctg aacaagaata cagggaaaga agtctctgaa attcgcttca tcgcacgaga
      721 aaaaatgacc gggcttgaaa gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca
      781 tcaccccgtg cgaggtgatg ttccgcttgt tgctaattta gtgaccaatc attggcgctt
      841 gtggaattaa gcgtcggtac aattcctccg gcaccgggct ttgccatact cccgcataca
      901 ttgcgtaacc aatcaccgca aacataatcc ccagaaccag tagcgtcatt aaccagccag
      961 acaacgcaaa ggctttttta tttgctgcag gtttttgcag tgaaaaggtc aatgttgagg
     1021 ctaccggaca tgactctacg caagtcatac agccggtaca ttccactgtt cgtacctgaa
     1081 ttaatttatc taccgggatc cgtgatgggc acttttttgc gcatttgcca caatcgatac
     1141 aactttcggc attgcgacga atcttaaacg gcgacaatag cgaaaccacg cccatcagcg
     1201 cgccatatgg gcaaagataa cgacaccagg catggcgaat aaacaggctg gcaatcagca
     1261 aaacggtcac gctgattaat gtcgcggtcc ccatatgacg aaagaaatcg agcattttaa
     1321 cgtccatcac cacgctatag ggcgacaaca taaaatagtg aatcgcctga gcgggcatca
     1381 ataacgcgat atagagaaat aaactcaaca gcaaatactt cacgccgcgc agaggaatat
     1441 ccagccagcg gggaaggaca cattgccgac caaacagttt gttaccgaga tcgccgatta
     1501 attcagaaag cgtaccaacc gggcataacc atgagcaaaa ggcctttttg agtaatagac
     1561 tgatgacgat aaaagcgacc aataacagca tcgcggcggc gtggacggac ggtaactgac
     1621 ctgttgcaag gctatatttc agattcatca gaccggcaat cggtagccag ccttcgatac
     1681 ctcccggtct ggcgacaaat gtcgtactac ctgccgtttc gtaatagcgc acccaatacc
     1741 agaacgtgat ggcaatataa atattcattg ccaatagtaa gaattgcgtc gctttacgcc
     1801 aggtcgtggc attgcgccag tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc
     1861 gctgccagcg ggttcttttt ttctctgcca tgattttgcc agtccgttaa gttgcatacc
     1921 aaatgccact attctagtta ttcttaactg gctgatattg attcaaatcg cgttcaggtc
     1981 tttcttattc aatcatgctt ccagagcggc aacactgcgt gtaatttctt cgtgtggaag
     2041 gggggcagat aatggctgct gctccagttg tgcgcatagc tgactggcgc tacgcattcc
     2101 cagactcgag caactgcttt ttagctgatg cgcggcacgc tttattttct cgctgtcctg
     2161 gctggcgcgg gcaatgtcga tttcatcgag aagcggcagg gcatgttgtt taaataatgc
     2221 cagccattcg tggatcttct ccgtccccat taactgagcg tcttcattga gttgcgagac
     2281 atccagtggt agatcgttat tggcttgcag ttggagatag tgcgccagta actgaccgag
     2341 cacttcacgc ggcaccggtt tagggataat cccgcgaaat agtgaactgg tacgctggcg
     2401 cagcgtttcg tcaatgacat gggcgctaaa gccaatcaaa accagcgacg gatattgctg
     2461 tgccagttgc cgggcaaggg taatgccgtc gatatccggc agatcaaaat ccaccagtgc
     2521 ggcagcaaac ggttcgctat tttgcagtgt ctctaaagcc tgcgcggcat tgccaatagc
     2581 aacaacctgc gcaccactgg tgttcagcat ctcaacggta attcgctggg ttagcgggtt
     2641 atcttcaatt aacagcaaac gtaaaccgtc aagacgcacc gcctgattgg ctgtttttgg
     2701 cacgggtgca gtggcaacac gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg
     2761 cgtgctggtg gcgctcagtt cgccgcccat cgcctgggcc agacggctac tgatagtcag
     2821 ccccagcccg gtgccgccgc gtttgccgct tacctgcaca aatggctgga agatttctgc
     2881 cagtttcgcg ggatcaatac cgcagccgct gtcttccact tcgaccagcc attgctcgcc
     2941 atcagtgcga ctacgcagga taatgtgccc ttcgtcagta aaacgcaggg cattgctcaa
     3001 taggttggta atgacctgac gaatacgtcg tggatcgccc attaacgcgc tcggtacatc
     3061 atcggcaatt gccgttgcca ggcgaatcgg gcgacctttc acccgtccgc tcattaattg
     3121 cagggtactt tccagcagcg ggcgcggttc aaagggctca tcgctgactg aaacattttt
     3181 gccacctgct tcgatagccg aataatcgag aatatcgttg aggatggtca gcaacgattc
     3241 gccagagtcg gtaatcgcct gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc
     3301 cagcagttga gcagtgccga gaataccgta cagcggtgtg cggatctcat ggctcatcgc
     3361 cgtcagaaac gccgattttg cctggctggc tttttctgct tccgcccgtg cctgtcggtg
     3421 ttctatcacc agttcctgca attcagctgt acgcgctttg acctgcgccg ccagctgttc
     3481 gcggtggcga ttcagtgcat gaacactgct gcgaaacgca tccatcagcc gcccgatggt
     3541 atccagctcc cgtacgccag cggtttccgg gaaaggggag tcaatatcac cgtccagcag
     3601 ccgttgcagc gcctgcgttt gttcggcaag tggacgcgtg aatgagcgat aaaccacgcg
     3661 ccagaggatc ataatcagtg cgcaaagtga aaccatcccc agcaataaca ggctgtattg
     3721 cccgcgtgca ctggcttttt ccagatgtgc cagtccatgc tgattgtgca gctcaatggt
     3781 gtcgaccagc tgactgactt cgctactaaa ctgcgcgaac tgggcgatat tattttgggc
     3841 gagagtttgc aggtgattgc tgatttcact gtcctgctgg aacagcgcca acagatcgct
     3901 atattggcta acggtagtta acgttgttgc gacctgcgca cgaactcccg gatcttcaat
     3961 gcgtatttgc cgacgttgca gaattttcac cgcattattg agctgctttt ccagcgttgg
     4021 tgcatttttc tggatctgct ccagccccag attcatcacc atttgctgca cccgcagagc
     4081 gctaaggcgc agttcattca tctggttaac atactcaaga tcgatatcaa tcagccgatc
     4141 gagtgcactt tcagcagcct gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg
     4201 ggtcgctcca gcggaagttg tcgcattatt cgcctgacct tgctccaggc gtgcgatctc
     4261 atcggcggca gctatctgct gactgagttg ccgttgttgc tggcgtagtt gcagacgccg
     4321 ccccaccagt tccccttgct gacgtaatga acgggagatc tcctgctcct gttgttcaat
     4381 agcggtggta tcaaaacctt gttcccgtaa cgcttgcagc aacgcattaa tcttcaggct
     4441 ttatgcggtg agcattcgcc cctgcgcctg ccacatcttt tcgttatcgg cactggtcag
     4501 gttctgcgcg gcgaaaagtt cccaggcgct ggcttcgctc aactggcgcg ccatattcat
     4561 ggtaggaatc aatgcctgag tattgtcttt ttccacctgg ctgataaagc gcaggttgta
     4621 ccatcccacc agggtactgg tcagggttaa cagcgccatc agggcaaagc ccatccagag
     4681 tcttcgggtc agggttaaat tcacggtcgg tgcacttaag gtgaaaaagg ttgagtcgca
     4741 aagtggaatg catctagcat aaagccttat tattgatgag gctatcatgc gcgtactgct
     4801 atttttactt ctttcccttt tcatgttgtc ggcattttcg gctgataacc tgttgcgctg
     4861 gcatgatgcg cagcatttca cggtgcaagc ctctatgccg cttaaagcca aacgcgtatg
     4921 gaaactgtgc gcgctttatc ccagcctgaa agattcatac tggttatcgt tgaactatgg
     4981 tatgcaggag gctgctcgcc gctacggtgt ggatttaaaa gtgctggagg caggcggcta
     5041 cagccagttg gctacccagc aagcacaaat cgaccagtgt aaacagtggg gcgcagaggc
     5101 cattttgctc ggtagtagca cgacgtcatt tcccgacctg caaaagcagg tagcaaatct
     5161 gccggtgatc gaactggtaa atgctcttga tgatccccag gtgaaaagcc gcgttggtgt
     5221 accctggttt cagatgggct atcaacctgg gcgatatttg gtgcaatggg cgcacggtaa
     5281 accactgaac gtgctgctga tgcccggacc cgataacgcc gggggcagta aggagatggt
     5341 cgagggtttt cgcgcagcca ttgccggaag cccggtgcgt attgttgata ttgcgcttgg
     5401 tgataacgat attgaaatcc agcgcaacct gttgcaggag atgctggaac gccacccaga
     5461 aatcgacgtc gttgccggaa cggccattgc ggcagaggcg gcaatgggtg aagggcgtaa
     5521 cctgaaaacg ccgcttaccg tggtgtcgtt ttatctttca catcaggtgt atcgcgggct
     5581 gaagcgggga agagtgatta tggctgccag cgatcaaatg gtctggcagg gggaactggc
     5641 agttgagcag gccatcaggc aattacaggg gcaatcggtg tctgataatg tcagcccacc
     5701 gattttagtt ctgacgccga aaaatgccga ccgagaacat atccgccgct cgctgtcacc
     5761 ggggggattt cgtccggtct atttttatca gcacacatca gcggctaaga aataaccttc
     5821 accatgttgc gtcaccagta aatccgcgct gagttcatga cgtaaacgac gaattaacac
     5881 atcgacggtg cgcaggtcag ggttttccac ccgacgcgca gaaagcatac gcagcagacg
     5941 ctcacggctg agaatttcgc ccggattagt cacaaatgcc accaacattt catactctgc
     6001 gcgggtcagt ttaatcggct cgccatcccg ctccagcgta tggcgcgaca cattcaggca
     6061 ataacccgca aagcgatagc agtagtcctg agtgtgcggt tgagcttgtc gcgcgaggtc
     6121 gattcgccag agcagatttt tcacccgtac taccagttcg cgcagttcca gcggtttggt
     6181 gacgtaatcg tctgcgccca tttccagccc aacaatacgg tcaatccgat cgctgcgtcc
     6241 ggtaaccaga ataatcccca ccgttgagcg ttctcgcagg gcgcgggtta acatcaggcc
     6301 attttcatcg ggtaagttga tatccagcag aattaaatct accggctgat tctgcataat
     6361 ttcccgtagc ccggcaccgc tcgccgtaac ggaaacggta tacccctcct gagtgaagta
     6421 ggattgtaat cgcgcctggg taaccggctt atcttcaaca ataacaatgt gatgtggcat
     6481 cagagggttt tactcattct gttcatatct gttcatattt gctcattaag atcgcttcac
     6541 taaaccataa ttctacaggg gttattatgc ggaaactctg gaacgcgcta cgccgaccca
     6601 gtgctcgttg gtcggtactg gcgctggtcg caattgggat tgtgattggc attgcgctga
     6661 ttgtattgcc acacgttggg atcaaagtca ccagcacaac cgaattttgt gtcagttgcc
     6721 acagtatgca accggtgtat gaagaatata aacagtcggt gcatttccag aacgcctccg
     6781 gcgtgcgagc tgaatgccat gactgtcata tcccgccgga tattccaggc atggtgaagc
     6841 gcaaactgga agcgagcaac gacatttacc agacctttat tgcccactcc attgatacac
     6901 ctgaaaaatt cgaagccaaa cgcgcggaac ttgccgagcg tgaatgggcg cgaatgaaag
     6961 aaaacaactc ggcaacctgt cgctcctgcc ataactacga tgcgatggat catgccaggc
     7021 agcatcctga agcggcacgt cagatgaagg tggcagcgaa agataatcaa tcctgcatcg
     7081 actgtcataa aggtattgcc caccagttac cggatatgag tagcggcttc cgtaagcagt
     7141 tcgatgagct gcgcgccagt gctaatgaca gtggtgacac gctgtactct attgatatta
     7201 agccaattta tgcggcgaaa ggcgataaag aagcctctgg ttctctgctg cctgcttcgg
     7261 aagtgaaagt ccttaaacgt gacggcgact ggctgcaaat tgaaattacc ggctggacgg
     7321 aaagcgccgg acgtcagcgt gtactcaccc aattcccagg taaacgcatc tttgttgcct
     7381 cgattcgtgg tgatgtgcag cagcaggtga aaacgctgga gaaaaccacc gttgccgaca
     7441 ccaataccga gtggagcaag ttgcaggcca ccgcgtggat gaagaaaggc gacatggtga
     7501 acgatatcaa accgatctgg gcctatgcgg attcgttgta caacggcacc tgtaacgagt
     7561 gccacggcgc accggaaatc gcccactttg acgctaacgg ttggatcggc acgctcaacg
     7621 gcatgattgg ctttaccagt ctcgataaac gtgaagaacg cactttgttg aaatatctgc
     7681 aaatgaatgc gtctgacacc gcaggtaagg ctcacggcga taagaaggaa gaaaaataat
     7741 gaacaataac gatctctttc aggcatcacg tcggcgtttt ctggcacaac tcggcggctt
     7801 aaccgtcgcc gggatgctgg ggccgtcatt gttaacgccg cgccgtgcaa ctgcggcgca
     7861 agcggcgact gaggctgtca tctcgaaaga gggcattctt accgggtcgc actggggggc
     7921 tatccgcgcg atggtgaagg atggtcgctt tgtggcggca aaaccgttcg aactggataa
     7981 atatccgtcg aaaatgatcg ccggattgcc ggatcacgta cacaacgcgg cacgtattcg
     8041 ttatccgatg gtacgcgtgg actggctgcg taagcgccat ctgagcgaca cctcccagcg
     8101 cggtgataac cgttttgtgc gcgtgagctg ggatgaagcc ctcgacatgt tctatgaaga
     8161 actggaacgc gtacagaaaa ctcacgggcc gagtgccttg ctgaccgcca gtggttggca
     8221 atcgacgggg atgttccata acgcttcggg gatgctggcg aaagctattg ccttgcatgg
     8281 taatagcgtt ggtacgggcg gagattactc taccggtgct gcgcaggtga tcctgccgcg
     8341 cgtagtcggt tcaatggaag tgtatgaaca gcaaacctcc tggccgctgg tattgcagaa
     8401 cagcaaaacc attgtgctgt ggggctccga tttgctgaaa aaccagcaag cgaactggtg
     8461 gtgcccggat cacgatgttt atgaatatta cgagcagcta aaagcgaaag tcgccgccgg
     8521 tgaagttgag gtcatcagca tcgatccggt tgtcacatcc acccatgagt atctggggcg
     8581 cgagcatgtg aagcacattg cggttaaccc gcaaactgac gtgccgctgc aactggcgct
     8641 ggcacatacg ctgtacagtg aaaacctgta cgacaaaaac ttcctcgcta actactgtgt
     8701 gggttttgag cagttcctgc cgtatctgct gggtgagaaa gacggtcagc cgaaagatgc
     8761 cgcatgggct gaaaaactga ccggcattga tgccgaaacc attcgtgggc tggcgcggca
     8821 gatggcggcg aacagaacgc aaattattgc tggctggtgc gtgcagcgta tgcagcacgg
     8881 tgaacagtgg gcgtggatga ttgtggttct ggcggcgatg ctggggcaaa ttggcctgcc
     8941 aggtggtggt tttggctggc actacaacgg cgcaggcacg ccggggcgta aaggcgttat
     9001 tctgagtggt ttctccggct ctacgtcgat tccgcctgtt cacgacaaca gtgactacaa
     9061 aggctacagc agcactattc cgattgcccg ttttatcgat gcgatcctcg aaccggggaa
     9121 agtgatcaac tggaacggta aatcggtaaa actgccgccg ctgaaaatgt gtatttttgc
     9181 cggaactaac ccattccatc gccatcagca gatcaaccgc attattgaag gctggcgcaa
     9241 gctggaaacg gttatcgcca tagataacca gtggacctca acctgccgct ttgccgatat
     9301 cgtgctgcct gcgaccacgc agtttgagcg taacgatctc gaccagtacg gcaaccactc
     9361 caaccgtggc attatcgcca tgaaacaggt ggtgccgccg tagttcgagg cgcgcaacga
     9421 ctttgatatt ttccgcgagc tgtgccgtcg ctttaatcgt gaagaagcct ttaccgaagg
     9481 gctggacgaa atgggctggc tgaaacgcat ctggcaggaa ggtgtacaac aaggcaaagg
     9541 acgcggcgtt catctgccag cgtttgatga cttctggaat aacaaagagt acgtcgagtt
     9601 tgaccatccg cagatgtttg ttcgccacca ggcattccgc gaagatccgg atctcgaacc
     9661 gctgggcacg ccgagtggcc tgattgagat ctactcgaaa accatcgccg atatgaacta
     9721 cgacgattgt caggggcatc cgatgtggtt tgagaaaatc gaacgctccc acggtgggcc
     9781 cggctcgcag aagtatccgt tgcatctgca atctgtgcat ccggatttcc gacttcactc
     9841 gcagctatgt gagtcggaaa cgctgcgtca gcaatatacg gtagcgggta aagagccagt
     9901 gttcattaac ccgcaggatg ccagcgcgcg cggtattcgt aacggtgatg tggtacgcgt
     9961 ctttaacgct cgcggtcagg tgttggcagg agcagtagtt tctgaccgct atgcacccgg
    10021 cgtggcgcga attcacgaag gggcatggta cgatccagat aaaggcggcg agcctggtgc
    10081 gctgtgcaaa tacggtaacc ccaacgtgct gaccatcgac atcgggactt cgcagctggc
    10141 gcaggcgacc agtgcgcaca ctacgctggt ggaaattgag aagtacaatg gaacagtgga
    10201 gcaggtgacg gcgtttaacg gccccgtgga gatggtggcg cagtgcgaat atgttcccgc
    10261 gtcgcaggtg aaatcatgac cacgctgaca gcacaacaga ttgcctgtgt ttacgcctgg
    10321 ctggcgcagt tgttctcccg tgaactggac gatgaacaac tgacgcaaat cgccagtgcg
    10381 cagatggctg aatggttttc gttgctgaaa agcgaaccgc cgctcactgc ggcggtgaat
    10441 gatctggaaa acagtattgc cacg
//
LOCUS       DAZSIO010000139        10417 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZSIO010000139 DAZSIO010000000
VERSION     DAZSIO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.139"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            2..1369
                     /gene="dcuC"
                     /locus_tag="ACHX5T_003171"
     CDS             2..1369
                     /gene="dcuC"
                     /locus_tag="ACHX5T_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR8468252.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1412..1909)
                     /gene="sspB"
                     /locus_tag="ACHX5T_003172"
     CDS             complement(1412..1909)
                     /gene="sspB"
                     /locus_tag="ACHX5T_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR8468253.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(1915..2553)
                     /gene="sspA"
                     /locus_tag="ACHX5T_003173"
     CDS             complement(1915..2553)
                     /gene="sspA"
                     /locus_tag="ACHX5T_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR8468254.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(2948..3340)
                     /gene="rpsI"
                     /locus_tag="ACHX5T_003174"
     CDS             complement(2948..3340)
                     /gene="rpsI"
                     /locus_tag="ACHX5T_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR8468255.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3356..3784)
                     /gene="rplM"
                     /locus_tag="ACHX5T_003175"
     CDS             complement(3356..3784)
                     /gene="rplM"
                     /locus_tag="ACHX5T_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR8468256.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4003..5130)
                     /gene="zapE"
                     /locus_tag="ACHX5T_003176"
     CDS             complement(4003..5130)
                     /gene="zapE"
                     /locus_tag="ACHX5T_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR8468257.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5324..5722
                     /gene="zapG"
                     /locus_tag="ACHX5T_003177"
     CDS             5324..5722
                     /gene="zapG"
                     /locus_tag="ACHX5T_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR8468258.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            5876..7223
                     /gene="degQ"
                     /locus_tag="ACHX5T_003178"
                     /pseudo
     CDS             5876..7223
                     /gene="degQ"
                     /locus_tag="ACHX5T_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            7313..8380
                     /gene="degS"
                     /locus_tag="ACHX5T_003179"
     CDS             7313..8380
                     /gene="degS"
                     /locus_tag="ACHX5T_003179"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR8468259.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8442..9380)
                     /gene="mdh"
                     /locus_tag="ACHX5T_003180"
     CDS             complement(8442..9380)
                     /gene="mdh"
                     /locus_tag="ACHX5T_003180"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR8468260.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            9815..10285
                     /gene="argR"
                     /locus_tag="ACHX5T_003181"
     CDS             9815..10285
                     /gene="argR"
                     /locus_tag="ACHX5T_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR8468261.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2546 a         2654 c         2611 g         2606 t
ORIGIN      
        1 aatgttcggc ataattatat ctgtcatcgt attaattacg atgggctatt tgatcctgaa
       61 aaactacaaa cctcaggtgg tgctggctgc cgcaggtgtc ttcctgatga tgtgcggagt
      121 ctggttaggg ttcggtggtg tactcgatcc cgccaaaagc agcggctact tgatcgtcga
      181 catttataat gaaatcctgc gcatgctgtc caaccgcatt gccggattgg ggctgtcgat
      241 tatggcggtg ggcggttatg cccgctacat ggagcgcata ggagccagtt gcgcgatggt
      301 gagcttgcta agccgcccgt taaaactcat tcgctcgccg tatattattc tgtcggcaac
      361 ttacgtcatc ggccaaatca tggcgcagtt tattaccagc gcctccggtc tgggtatgtt
      421 gctgatggtc accttatttc cgacgctggt gagtctggga gtaagtcgcc tctctgcggt
      481 ggcggttatc gcaaccacga tgtccattga gtgggggatt ctggaaacga actccatttt
      541 tgcagcccag gtcgcgggaa tgaaaattgc cacatacttc ttccactacc agcttccggt
      601 cgcctcttgc gtcattatct cggtggcgat ctcccacttt ttcgtgcaac gcgcttttga
      661 caaaaaagat aaaaatatca atcacgaaca ggcagagcaa aaaactctcg ataatgtccc
      721 gccgctctat tacgccattt tacctgtgat gccgttaatc ctgatgctcg gctcgctgtt
      781 cctcgcccac gtcgggctga tgcagtcaga actgcatctg gtggtggtga tgttactaag
      841 tttgacggtg acgatgtttg ttgagttctt ccgcaagcat aacttgcgcg aaacaatgga
      901 cgatgtgcag gcgttttttg acggcatggg tacgcagttt gtcaacgtgg taacgctggt
      961 ggtcgcgggt gaaatatttg cgaaaggctt aacgacgatt ggcactgttg atgcggttat
     1021 caggggtgcg gagcattctg gtctgggcgg tattggcgtg atgattatta tggcgctagt
     1081 cattgccatt tgtgccattg tgatgggctc tggcaatgcg ccgtttatgt catttgccag
     1141 tcttattccg aatatcgcag ccggactaca tgtaccagcg gttgtaatga ttatgccgat
     1201 gcattttgcc acgacgctag cgcgcgcggt ttcgccgatt actgcggtgg tggtcgttac
     1261 gtcaggaatt gcaggcgttt cgccttttgc ggtggtgaag cggacagcga tccccatggc
     1321 agtcggtttc gtggtgaata tgattgccac aatcacgcta ttttattaag tcattaaaaa
     1381 gacaaaacag gccgcctggg cctgttttgt attacttcac aacgcgtaat gccggtcgac
     1441 caccgcgtgg tggctgcgga ggttcatcgt caggatgagt gtcatcatcg tgatctggct
     1501 tgtcgccatc aataaccgac ataacggttt cgttgtctgc cgatgcctct tcatcattca
     1561 tgatgctggt atcttcatcg taggcagctt caggctcaaa catcgtgcct gcgccatttt
     1621 cacgggcgta gatagccagc acggcagcca gcggcacaga aacctgacgc ggaatgccac
     1681 caaagcgcgc gttaaagcgc acctcatcat tcgccagttc cagattgccg acagcacgcg
     1741 gcgcaatgtt gagtacgatt tgcccgtcac gcgcatattc cataggaacc tgcacgccag
     1801 ggagcgtcac atccaccacc aggtgcggcg tgagctggtt atccagcaac cactcataga
     1861 atgcacgcag cagataggga cgacgtggtg ttagctgtga caaatccata cagattaact
     1921 ccggcccaga cgcatttcac gttctgcttc agttaaagaa gcaaggaaag agtcacgctc
     1981 aaagacgcgg gtcatatagc ctttcagctc tttcgcaccc gggccgctga actcgatgcc
     2041 cagttgcggc agacgccaca gcagcggagc aaggtagcaa tcgaccaggc tgaactcatc
     2101 gctcaggaag tacggcttct gaccgaagac cggcgcaatc gccagcagtt cttcgcgcag
     2161 ttgcttacgt gcggcatctg cttcagaagc tgaaccgttg atgatagtgt tcatcagcgt
     2221 gtaccagtct ttttcgatgc gatgcatgta cagacggctt tcaccgcgag ctaccgggta
     2281 aacaggcatc agtggcggat gcgggaaacg ctcatccaga tattccataa tgatgcgaga
     2341 ttcccacagg gtcagctcac gatccaccag ggtcggaacg ctctgattcg ggttgaggtc
     2401 aatcagatcc tgaggcggat tgtccttttc cacgtgttcg atctcgaaac ttacaccttt
     2461 ctcagccagc acaatgcgga cctgatggct atagatgtca gtaggaccgg aaaacagcgt
     2521 cattaccgaa cgtttgttgg cagcgacagc catgaaaacc tccaggtata gtcagaattt
     2581 ttactgctac cagccaccag gtggccagtc agaagttgtg ttacccaata aggaacgact
     2641 ctctttgttc gaaaatcaaa caaaaaatga gcaatacccg acatttgggc agaaaattgg
     2701 atgatagttt accagatttt gcgacctttg tggtgagtgg atgccggaaa tggggaaaaa
     2761 gagatgcgct ttagtctgaa atagttgact tagaccctta ttggcgatgt ggtttttgtt
     2821 ttacctgtct ttcagacggc agcaaaaagc aacttcccat tttttacgct gattcagaat
     2881 ttagccataa aaaaacccgc cgaagcgggt tttttcgcaa attgttttct gccgtagcag
     2941 aagccaatta acgtttggag aactgcggac gacgacgtgc tttacgcaga ccgactttct
     3001 tacgttcaac ctgacgagcg tcacgagtaa cgaagccagc tttacgcagt tcagaacgca
     3061 gggactcgtc gtattccatc agagcgcggg tgataccgtg acggatcgca ccagcctgac
     3121 cagagatacc accaccttta acggtgatgt acaggtccag tttctcaacc atgtcgacca
     3181 gttccagcgg ctgacgaact accatgcggg cagtttcacg accgaagtac tgttccagag
     3241 aacgttggtt gattacgatt ttaccgttgc ccggtttgat gaaaacgcga gctgcggaac
     3301 ttttgcggcg accagtgccg tagtattgat tttcagccat tgcctataat cccgattaga
     3361 tgtcaagaac ttgcggttgc tgtgccgcgt ggttgtgctc gttacccgcg taaactttca
     3421 gtttacggaa catagcacga cccagcgggc cttttggcaa catgccttta accgcgattt
     3481 caatcacacg ctcaggacgg cgagcaatca tctcttcaaa ggtcgcttgt ttgataccac
     3541 cgatgtggcc ggtgtggtga tagtacactt tgtcagtacg cttgttgccg gttacagcaa
     3601 ctttgtcagc gttcagaacg atgatgtaat caccggtatc tacgtgcgga gtatattccg
     3661 ctttgtgctt accgcgcagg cgacgagcca gttcagtagc cagacggccc agagttttac
     3721 cggtcgcgtc aacaacatac cagtcgcgtt ttacggtttc tggtttagct gtaaaagttt
     3781 tcattaaaag cttacccaat aaatagttac acgttggtga acacccaaac gtcttcaatt
     3841 gttgaggttc acacgacaaa gtccggcaaa cctacccctt cgaatagcct atgccagcac
     3901 acaaaaagtt ttgggaaaaa aactttcttg taacgtgggg tcgcaggatt atagagaagt
     3961 cggggtcaaa gatcgacccc tttttgtgat ttgtggcagg ttttaacccg ccaaatgctc
     4021 gcgcttcaga tactcttcgc tttgcatctc ttgcagacgt gacaggcaac gctggaactc
     4081 aaacttcagc cgctcaccct gataaatttc atacagcggc acttctgcac tcaccactaa
     4141 tttgacatgg cgctcgtaaa actcatccac cagcgcaata aagcgccgcg cttcgctctc
     4201 catcaaccgc gtcatgactg gcacatcaaa caacattact gtgtgaaaca ggcgtgagag
     4261 cgcaatatag tcatgctgac tgcgggcgtc gacgcacagc gtagtaaaag aaaccgccag
     4321 cgtctggttc tcgacgccca ttgtcgccaa tggccgatgg ttgatttcta acgtcggtga
     4381 attttctcgt ttcacccccg ccagcgccaa ccatagttta tccatctgcg cccgggtttc
     4441 atcgttgagt ggcgaaagcc acagatgcgc ctgagtgagt gtacgcagtc gataatcaac
     4501 accagcgtcc acgttcatta catcacaatg ctgtttaatg gcatcgattg caggcagaaa
     4561 acgcgcacgt tgcaggccat ttcgataaag ttcgtccggc ggaatatttg acgtcgctac
     4621 cagggtaata ccgcgggtga acagggcttt catcagaccg ccaagtagca tggcgtcggt
     4681 aatatcagaa acaaaaaatt cgtcaaaaca gagcacgtca gtttcggctt taaagcgatc
     4741 ggcaataatt tccagcggat cggtctgccc ctgtaaggca gttagctctt cgtgcacccg
     4801 cagcataaaa cggtgaaagt gcaggcgctg tttccgctct cccggcaggc tttgatagaa
     4861 aaggtccatc agccaggttt tcccgcgtcc tacaccgccc cacatatata agccacgcac
     4921 tggcgtatgc tttgtgtctt cgcgtttacc ccacagctta ccgacccgcg ccattagccc
     4981 actcgtcctg ggggctggtg gcctgctatt gatgagttct tgataaataa tttccaggcg
     5041 gctgacggcc tctttttgaa cgtcgtcggg ttgatggctg ccttcattga gcgccttcag
     5101 gtattgcgat gttggggtaa cgctttgcat gatcttattg ttattccttg aataatcggt
     5161 gcgccgttgt tcacggttga cgaaaaaaag gccgttctac actacgcgat atgcagtcgg
     5221 gattccactt ctgtggaatt aacggttata gtggcataat cagccgcagg catggagcct
     5281 gaagccaaca ccctacggaa acaaaagaca acgggagatg ttcatgacct gggaatatgc
     5341 gctaattggg ttagtcgtcg gcatcattat tggtgctgtg gccatgcgtt ttggtaatcg
     5401 taaactacgc cagcaacagg cgttgcagta cgaactggaa aagaataaag ctgaactgga
     5461 cgagtatcgc gaagagctgg ttagccactt tgcccgcagc gcggaattac tggataccat
     5521 ggcgcacgac tatcgccagc tgtatcagca catggcaaaa agctccagca gcctgctgcc
     5581 ggaactgtct gctgaagcaa acccgttccg taatcgtctg gcagagtctg aagccagcaa
     5641 cgatcaggca ccggtgcagg tgcctcgcga ctattctgaa ggcgcatccg gcctgctgcg
     5701 tactggcgcg aagcgcgact aatttatttt tcgggcgcag ccattgcgcc ctcctcttct
     5761 ctccctcccc gactatcatt taatctggtg tctcattgtt agccgtctga aaattcaata
     5821 acatcaaact gttttgaatc tcttttctta tcattcaggt acgagagcag gaataatgaa
     5881 aaaacaaacc cagctgttga gtgcattagc gttaagtgcc gggttaactc tctcggcgtc
     5941 atttcaggcc gtcgcgtcga ttccaggcca ggttgccgat caggcccctc tccccagtct
     6001 ggctccaatg ctggaaaaag tgcttccggc agtggtgagc gtacgggtgg aaggaacggc
     6061 cagtcaggga cagaaaatcc cggaagaatt caaaaagttt tttggtgatg atttaccgga
     6121 tcaacctgca caacccttcg aaggtttagg ttccggtgtc atcatcaacg ccagtaaagg
     6181 ctatgtgctg accaacaacc atgtgattaa tcaggcacag aaaatcagta ttcagctcaa
     6241 tgatgggcgc gagtttgatg caaaactgat tggtagcgat gaccagagcg atatcgccct
     6301 gttacaaatt caaaacccga gcaaattaac gcaaatcgct attgccgact ccgataaatt
     6361 gcgcgtcggt gattttgccg tagcggtcgg taacccattt ggccttgggc aaaccgccac
     6421 ctctggcatt gtttccgcat taggccgcag cgggttgaat cttgaaggtc tggaaaactt
     6481 tatccagaca gatgcttcca ttaaccgcgg taactccggc ggtgcactat taaaccttaa
     6541 cggtgagtta attggcatca acactgcaat ccttgcgcct ggcggcggga gcgtcgggat
     6601 tggatttgcc atccccagta atatggcgcg aacactggcg cagcagctta tcgactttgg
     6661 tgaaatcaaa cgcggtttgt taggcatcaa aggcaccgag atgagtgccg atatcgccaa
     6721 agccttcaac cttgacgtgc agcgtggcgc gtttgtcagc gaagtgttgc caagttctgg
     6781 ctcagcgaaa gcgggcgtca aagcgggcga tattattacc agcctcaacg gcaaaccgct
     6841 gaatagcttt gctgagttgc gctctcgtat cgcgactaca gagccgggca cgaaagtgaa
     6901 gcttggcctg ctgcgtaacg gcaaaccact ggaagtagaa gtgacgcttg ataccagcac
     6961 ctcttcgtcg gccagcgctg gaaggtgcaa cgttgagcga tggtcagcta aaagatggcg
     7021 gcaaaggtat taagatagat gaagttgtca aaggaagccc agctgctcag gctggcttgc
     7081 aaaaagacga tgtgatcatt ggcgtcaacc gcgatcgggt gaactcgatt gctgaaatgc
     7141 gtaaagtgct ggcggcaaaa ccggccatca tcgccctgca aattgtacgc ggcaatgaaa
     7201 gcatctatct gctgatgcgt taatgtcgta aaccgggcat caggctgacg tgtgatgtcc
     7261 ggttaactcg tggtatgctg ctgccgttcc cttttttaat gacgcctcca tcatgtttgt
     7321 gaagctctta cgttccgttg cgattggatt aattgtcggc gctattctgc tggttgccat
     7381 gccttcgctg cgcagcctta acccgctttc cactccgcaa tttgacagta ccgatgagac
     7441 gcctgccagc tataatctgg cggttcgccg cgccgcgcca gcggtggtta acgtttacaa
     7501 ccgtggtttg aacaccaact ctcacaacca gcttgagatc cgcaccctgg gatccggtgt
     7561 aatcatggat caacgcggtt atatcatcac caataaacac gtcatcaacg acgccgatca
     7621 gatcatcgtc gccttacagg atggacgtgt atttgaagca ttgctggtgg gatctgactc
     7681 tctaaccgat ctggcggtac ttaaaattaa tgccactggc ggtttaccta ccattccaat
     7741 taatgcacgt cgcgtaccgc acattggcga cgtagtactg gcgatcggta acccgtacaa
     7801 cctcgggcag accattaccc aagggattat tagtgccacg ggtcgaatcg gtctgaaccc
     7861 gaccgggcgg caaaacttcc tacaaaccga tgcttccatt aaccacggta actctggcgg
     7921 cgcgctggtg aactcgctgg gagaactgat gggcattaac acgctgtcgt ttgataagag
     7981 taacgatggc gaaacgccgg aaggtatcgg ctttgcgatt cctttccagt tagcaaccaa
     8041 aattatggat aagctgatcc gcgatggtcg cgtgatccgc ggctacattg gtatcggcgg
     8101 acgcgagatc gcaccactgc acgcgcagag cggtggtata gatcaactgc aagggatcgt
     8161 ggttaatgaa gtgtcacctg acggcccagc ggcgaatgcg ggtattcagg tcaacgatct
     8221 gattatttcg gtggataaca aaccggccat ctctgctctg gagacgatgg atcaggtggc
     8281 ggaaattcgc cctggttcgg tgatcccggt tgtagtgatg cgtgatgata agcagttaac
     8341 gctgcaggtc accattcagg aatatccggc aaccaattaa gtcgagtgct caaaacaaaa
     8401 accggagtct gtgctccggt tttttattat tcgctaatca attacttatt aacgaactct
     8461 ttgcccaggg cgatatcttt cttgagcgta tccagcatac cttccagcgc gttctgttca
     8521 aatgcgctca gggtaccgat agatttacgc tcttccacgc cgtttttacc cagcagcagc
     8581 ggttgagaga agaaacgggc gtactgaccg tcgccttcaa cgtaggcaca ttcgacaacg
     8641 ccttgttcgc cctgcagtgc acgaaccaga gacagaccaa aacgtgcagc tgcctggccc
     8701 atagacaggg ttgcagaccc gccaccggct ttcgcttcaa ccacctcagt acccgcgttc
     8761 tggatacgtt tggtcagatc agccacttcc tgctcggtaa aactaacgcc aggaacctgt
     8821 gacagcagcg gcagaatggt aacaccagag tgaccgccaa taaccggcac ttcaacttcg
     8881 cctggctgtt tgcctttcag ttccgcaaca aaggtgttgg aacgaatgat atccagcgtg
     8941 gtaacgccga acagtttgtt tttgtcataa acaccggctt ttttcagcac ttcagcagca
     9001 attgcaactg tggtgttaac cgggttagtg ataataccaa tgcacgcttt cgggcagttt
     9061 ttcgcaactt gctgtaccag gtttttcacg atgccggcgt taacgttaaa caggtcggaa
     9121 cgatccatac ccggtttacg cgctacgcct gcagagataa gaacgacatc tgcgccttcc
     9181 agcgccggag tcgcatcttc accagaaaaa cctttgattt tcacagcagt agggatatgg
     9241 ctcagatcga cggccacacc gggagtcact ggagcgatat catacagaga gagttctgaa
     9301 cctgaaggca gttgggtttt taacagtagt gcaagcgcct ggccaatacc gccagcagcg
     9361 ccgaggactg cgactttcat cctaaactcc ttattatatt gataaactaa gatatgttgc
     9421 tccgctgccg cgaccttaat ccacaaaatt gccgtttaca atgaccacat ctcaagaatg
     9481 tgtagtcacg caagtttagc gtttatgcat ttaattgccg taatcaggaa cctaacttac
     9541 gtaattaaca gtcgcttatg cattagcgca acattccaac aggtggtgac aatataccct
     9601 accgttcagc caaaacaaca tcaatttgat aacaattaat ttacttttaa acagaatttg
     9661 catgccgtga cgcaggcatg tttctcaata acgaaatttg ataaaatccc gctctttcat
     9721 aacattattt cagccttctt cagggctgac tgtttgcata aaaattcatc tgtatgcaca
     9781 ataatgttgt atcaaccacc atatcgggtg acttatgcga agctcggcta agcaagaaga
     9841 actagttaaa gcatttaaag cattacttaa agaagagaaa tttagctccc agggcgaaat
     9901 cgtcgccgcg ttgcaggagc aaggctttga caatattaat cagtctaaag tctcgcggat
     9961 gttgaccaag tttggtgctg tacgtacacg caatgccaaa atggaaatgg tttactgcct
    10021 gccagctgaa ctgggtgtac caaccacctc cagtccattg aagaatctgg tgctggatat
    10081 cgactacaac gatgcagttg tcgtgattca taccagccct ggcgcggcgc agttaattgc
    10141 tcgcctgctg gactcactgg gcaaagcaga aggtattctg ggcaccatcg ctggcgatga
    10201 caccatcttt accacccctg ctaacggttt caccgtcaaa gacctgtacg aagcgatttt
    10261 agagctgttc gaccaggagc tttaatctct gccccgtcgc ttctggcggc ggggaaaatg
    10321 ttgcttatcc ctctcattcc ccttctttct cctgcgatac atttaacgaa tagtgcgttt
    10381 taccgcgaca catcattcac aaaatgaata catacgg
//
LOCUS       DAZSIO010000140        10174 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZSIO010000140 DAZSIO010000000
VERSION     DAZSIO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10174
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.140"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            413..1279
                     /gene="folD"
                     /locus_tag="ACHX5T_003182"
     CDS             413..1279
                     /gene="folD"
                     /locus_tag="ACHX5T_003182"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR8468262.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1281..1493
                     /gene="ybcJ"
                     /locus_tag="ACHX5T_003183"
     CDS             1281..1493
                     /gene="ybcJ"
                     /locus_tag="ACHX5T_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR8468263.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1601..2110
                     /locus_tag="ACHX5T_003184"
     CDS             1601..2110
                     /locus_tag="ACHX5T_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR8468264.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVLAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(2146..3531)
                     /gene="cysS"
                     /locus_tag="ACHX5T_003185"
     CDS             complement(2146..3531)
                     /gene="cysS"
                     /locus_tag="ACHX5T_003185"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR8468265.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3705..4199
                     /gene="ppiB"
                     /locus_tag="ACHX5T_003186"
     CDS             3705..4199
                     /gene="ppiB"
                     /locus_tag="ACHX5T_003186"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR8468266.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            4202..4924
                     /gene="lpxH"
                     /locus_tag="ACHX5T_003187"
     CDS             4202..4924
                     /gene="lpxH"
                     /locus_tag="ACHX5T_003187"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR8468267.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            5042..5551
                     /gene="purE"
                     /locus_tag="ACHX5T_003188"
     CDS             5042..5551
                     /gene="purE"
                     /locus_tag="ACHX5T_003188"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR8468268.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            5548..6615
                     /gene="purK"
                     /locus_tag="ACHX5T_003189"
     CDS             5548..6615
                     /gene="purK"
                     /locus_tag="ACHX5T_003189"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR8468269.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6752..7645)
                     /locus_tag="ACHX5T_003190"
     CDS             complement(6752..7645)
                     /locus_tag="ACHX5T_003190"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR8468270.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7642..8289)
                     /locus_tag="ACHX5T_003191"
                     /pseudo
     CDS             complement(7642..8289)
                     /locus_tag="ACHX5T_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            8292..9524
                     /gene="allC"
                     /locus_tag="ACHX5T_003192"
     CDS             8292..9524
                     /gene="allC"
                     /locus_tag="ACHX5T_003192"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFR8468271.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            9535..>10174
                     /gene="allE"
                     /locus_tag="ACHX5T_003193"
     CDS             9535..>10174
                     /gene="allE"
                     /locus_tag="ACHX5T_003193"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFR8468272.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2368 a         2726 c         2688 g         2392 t
ORIGIN      
        1 gatagtagta aaattccttt ttatcctcta agaatgtctt aattgaaaat atgcactcta
       61 ttctaaaaaa tagagagccc cgttagatga atacttccgc gcaaaatata ttcaccacaa
      121 atatagacct gaagcggtaa attaccaggc tgaaaattct ttttatattg tcaggtattt
      181 cttaaattat cttaatcctt aaacaaggaa ataaatcagt tccagattta caacgccatc
      241 atgtacgaaa aatgaagctt tcaatctcag cgacctcagc gacggtgcgc ctaaccttcg
      301 caagaggtcg cttcacgcga taaatctgaa acgaaacctg acagcgcgcc ccgcttctga
      361 caaaatagac gcatcccctt cgacctacgt aacagatgga atcctctctc tgatggcagc
      421 aaagattatt gacggtaaaa cgattgcgca gcaggtgcgc tctgaagttg ctcaaaaagt
      481 tcaggctcgt attgcagccg gactgcgggc accaggactg gccgttgtgc tggtgggtag
      541 taaccctgca tcgcaaattt atgtcgcaag caaacgcaag gcttgtgaag aagtcgggtt
      601 cgtctcccgc tcttatgacc tcccggaaac caccagcgaa gcggagctgc tggagcttat
      661 cgatgcgctg aatgccgaca acaccatcga tggcattctg gttcaactgc cgttaccggc
      721 gggtattgat aacgtcaaag tgctggaacg tattcatccg gacaaagacg tggacggttt
      781 ccatccttac aacgtcggtc gtctgtgcca gcgcgcgccg cgtctgcgtc cctgcacccc
      841 gcgcggtatc gtcacgctgc ttgagcgtta caacattgat accttcggcc tcaacgccgt
      901 ggtgattggc gcatcgaata tcgtcggtcg cccaatgagc atggaactgt tgctcgcagg
      961 ttgcaccacc accgtgactc accgcttcac taaaaatctg cgtcatcacg tagaaaatgc
     1021 cgatctgttg atcgttgccg ttggcaagcc aggctttatt cccggtgact ggatcaaaga
     1081 aggcgcaatt gtgattgatg tcggcatcaa ccgtctggaa aatggcaaag ttgtgggcga
     1141 cgtcgtgttt gaagacgcgg ctaaacgcgc ctcatacatt acgcctgttc ccggcggcgt
     1201 tggcccgatg acggttgcca cgctgattga aaacacgcta caggcgtgcg ttgaatatca
     1261 tgatccacag ggtgagtaac atggcgacat tttctttagg taaacatccg cacgttgagc
     1321 tgtgcgactt gctgaaactg gaaggctgga gcgaaagcgg cgcgcaggcg aaaatcgcga
     1381 ttgccgaagg ccaggtgaaa gtcgacggtg cggttgaaac gcgcaaacgc tgcaaaatcg
     1441 tcgccggtca gacagtgagt tttgcaggac acagcgtaca ggttgttgcc tgatcccttt
     1501 cccggcctgt atcaacaggc cggttctctt ttcagacttt tgccctctac gccccgttca
     1561 tccatgctga tagcatcgac attttgccga agcgaccatc atgccaaccg ttattacgca
     1621 cgccgccgtt cccctttgca ttggtttagg gttgggttcg aaaggtctgt tatttgccgg
     1681 aatcatcctg gcgatgctgc ccgatgccga cgtattgtcg tttaaatttg gcgttgctta
     1741 cggcaatgtt tttggtcatc gcggttttac ccattcgctg gtgcttgcgt ttgttgtccc
     1801 gctcttatgt gtgctgattg gacgtcgatg gttcagggct gggctgattc gctgctggct
     1861 gtttttaacc gtctcgttgc tatcgcacag cttactggat tcggtaacca ctggcggtaa
     1921 gggcgttggc tggctgtggc cgtggtcaga tgaacgcttt ttcgctccct ggcaggtgat
     1981 taaagtcgcg ccgtttgcat tgtctcgtta caccacagcg tacgggcatc aggtgattat
     2041 ttccgaattg atgtgggtgt ggttgccggg gatgctactg atgggaatgt tgtggtggcg
     2101 cagacgataa ccggatgcga aaactcgcat ccggcaattg cgcaattact tacgacgcca
     2161 ggtggtccct tgcgggccat cttccagcac gatccccatc tcgttaagac ggtcacgtgc
     2221 cgcatctgcc gccgcccagt ctttcgcttt acgggcatcc agacgctgtt gaattaacgc
     2281 ttcaatctca gccacttcgc tgtcgtctgc ctgcgcgccg ctttgcagga acgcttccgg
     2341 ttcttgctcc agcaggccca atacagcgga aagtttacgc aggtgagatg ccattgcatt
     2401 cgccgctgcc atatcttctg ctttcagacg gttcacttca cgcgccatat caaacagcac
     2461 ggaataggct tccggggtgt tgaaatcgtc gtccatcgct tcaataaagc gcgcttcaaa
     2521 cgcttcgcca ccggcaggcg caacggtttt atctgtgccg cgcagcgcag tgtagaggcg
     2581 ctccagcgcc gcacgcgcct gcttcaggtt ctcttcgctg tagttcagct ggctgcgata
     2641 gtggcccgac atcaggaagt aacgcacggt ttccgcgtcg tagtatttca gcacatcgcg
     2701 cacggtaaag aagttaccca gcgatttgga catcttctcg cggtcaacca tcaccattcc
     2761 ggagtgcatc cagtagttca catactgacc atcatgggca caggtggact gcgcgatttc
     2821 gttctcgtgg tgcgggaaca tcaggtctga accgccgccg tggatatcaa agtggttacc
     2881 cagctgcttg cagttcatgg ccgaacattc aatgtgccag ccaggacgac ccgcgcccca
     2941 cggagacggc cagctcggtt cgccctcttt cgacatcttc cacagaacga agtccatccg
     3001 gttgcgtttg tcatcaacca cgtcaacgcg cgcgccagcc tgcagctggt cgagatcctg
     3061 acgcgacagc acgccataag ttggatcggt cgggacgtcg aacatcacgt cgctgttgtc
     3121 cgccacataa gcgtgacctt tggcgatcag ttgttcagtg agttcaataa tttctgcgat
     3181 atggtgcgtc gcgcgcggct ccatatccgg gcgcagaatg ttcaaagcat caaaatcttt
     3241 gtgcatttcg gcgatcatgc gatccaccag cgccacaaag ctttcgccat tttcattggc
     3301 gcgtttgatg attttgtcgt cgatatcggt aatgttgcgc acatacttca gtttatagcc
     3361 gaggaaacgc agatagcgcg caaccacgtc aaaagcaaca aaggtacgcc cgtgaccgat
     3421 atgacagaga tcgtaaacgg tgattccaca cacgtacatg ccgacttccc cggcgtgaat
     3481 aggcttaaat tcctcttttt ggcgtgtcag agtattgaag atttttagca tcgaagattc
     3541 cgtttagaca tgtgtgggta attgagttgc gtataatacc catatttccc gcgcgaatca
     3601 gcatacattg cgtgatgatc cgatcgtgcg gttatgctat aacaccaccc tatatatgac
     3661 ccgaactggg ttgaagcacc aatcaaacgg aacaggatgc aaaaatggtt actttccaca
     3721 ccaatcacgg cgatattgtc atcaaaactt ttgacgataa agcacctgaa acagttaaaa
     3781 acttcctgga ctactgccgc gaaggttttt acaacaacac cattttccac cgtgttatca
     3841 acggctttat gattcagggc ggcggttttg aaccgggcat gaaacaaaaa gccaccaaag
     3901 aaccgatcaa aaacgaagcc aacaacggcc tgaaaaatac ccgtggtacg ctggcaatgg
     3961 cacgtactca ggctccgcac tctgcaactg cacagttctt catcaacgtg gttgataacg
     4021 acttcctgaa cttctctggc gaaagcctgc aaggttgggg ctactgcgtg tttgctgaag
     4081 tggttgacgg catggacgtg gtagacaaaa tcaaaggtgt tgcaaccggt cgtaacggta
     4141 tgcaccagga cgtgccaaaa gaagacgtta tcattgaaag cgtgaccgtt agcgagtaat
     4201 cgtggcgaca ctctttattg cagatcttca tctctgcgtg gaagaaccgg cgatcaccgc
     4261 cggttttctg cgttttttag cgggggaagc ccgcaaggcc gacgcgctgt atattcttgg
     4321 cgatctgttt gaagcatgga ttggcgacga cgatcccaac ccactccatc gccagatggc
     4381 ggcagcgatc aaagcggtgt ccgattccgg cgttccctgt tatttcattc atggcaaccg
     4441 tgattttctg ctcggcaaac gctttgcccg tgaaagcggc atgacattat tgccggaaga
     4501 aaaggtgctc gaactttatg gtcgccgggt gttgattatg catggcgaca cgctgtgcac
     4561 cgatgacgcg ggttatcagg cttttcgcgc caaagtccac aaaccctggc tgcaaatgct
     4621 attcctcgcc ctgccgttgt ttgtgcgcaa acgcattgcc gcgcgaatgc gcgcgaacag
     4681 caaagaagcc aacagcagta aatcgctggc gatcatggac gttaaccaaa acgctgtggt
     4741 cagtgcgatg gaaaaacatc aggtgcaatg gctaatccac gggcataccc atcgcccggc
     4801 ggtgcatgaa cttatcgcca accagcaacc tgcttttcgc gtggtactgg gtgcctggca
     4861 tacggaaggt tcgatggtga aagtcacggc tgatgacgtt gagctgattc attttccgtt
     4921 ttaaaaaacc cacaactttg ctgatttcac agccacgcaa ccgttttcct tgctctcttt
     4981 ccgtgctatt ctctgtgccc tctaaagccg agagttgtgc accacaggag ttttaagacg
     5041 catgtcttcc cgcaataatc cggcgcgtgt cgccatcgtg atggggtcca aaagcgactg
     5101 ggctaccatg cagttcgccg tcgaaatctt cgaaatcctg aatgtcccgc accacgttga
     5161 agtggtttct gctcaccgca cccccgataa actgttcagc ttcgccgaaa gcgccgaaga
     5221 gaacggttat caggtgatta ttgcgggcgc aggcggcgca gcgcacctgc caggcatgat
     5281 tgccgccaaa acgctggtgc cggtgctggg cgtgccagta cagagcgccg cactgagcgg
     5341 tgtcgatagc ctctactcca tcgtacaaat gccgcgcggc attccggtgg gtacgctggc
     5401 gattggtaaa gctggcgcgg caaacgcggc attactggca gcacaaattc tcgcgactca
     5461 cgataaagag ctacaccagc gtctgaatgg ctggcgcaaa gcccagaccg acgaagtgct
     5521 ggaaaacccg gacccgcgag gtgcggcatg aaacaggttt gcgtcctcgg taacgggcag
     5581 ttaggccgta tgctgcgtca ggcaggcgaa ccgttaggca ttgctgtctg gccggtcggg
     5641 ctggacgctg aaccggcggc ggtgcctttt caacaaagcg tgattaccgc tgagatcgaa
     5701 cgctggccgg aaaccgcatt aacccgcgag ctggcgcgtc atccggcctt tgtgaaccgc
     5761 gatgtgttcc cgattattgc tgaccgtctg actcagaaac agcttttcga taagctccac
     5821 ctgccgaccg cgccgtggca gttacttgcc gatcgcagcg agtggcctgc ggtgtttgat
     5881 cgtttaggtg aactggcgat tgttaagcgt cgcactggtg gctatgacgg tcgcggtcaa
     5941 tggagtttac gcgccaatga aaccgaacag ttaccggcag agtgttacgg tgaatgtatt
     6001 gtcgagcagg gcattaactt ctctggtgaa gtatcgctgg ttggcgcgcg cggctttgat
     6061 ggcagcaccg tgttttatcc gctgacgcat aacctgcatc aggacggtat tttgcgcacc
     6121 agcgtcgctt ttccgcaggc caacgcacag cagcaggcgc aagccgaaga gatgctgtcg
     6181 gcgattatgc aggagctggg ctatgtgggc gtgatggcga tggagtgttt tgtcaccccg
     6241 caaggtctgc tgatcaacga actggctccg cgtgtgcata acagcggtca ctggacacaa
     6301 aacggtgcca gcatcagcca gtttgagctg catctgcggg cgattaccga tctgccgtta
     6361 ccgcaaccag tagtgaataa tccgtcggtg atgatcaatc tgattggtag cgatgtgaat
     6421 tatgactggc tgaaattgcc gctggtgcat ctgcactggt acgacaaaga agtccgtccg
     6481 gggcgtaaag tggggcatct gaatttgacc gacagcgaca catcgcgtct gactgcgacg
     6541 ctggaagcct taatcccgct gctgccgcca gagtatgcca gcggcgtgat ttgggcgcag
     6601 agtaagttca gttaagcagg tgctcatttg attgccggat gcggcgtaaa cgccttatcc
     6661 ggcttaccgc tccggtaccc attgtaggcc tgataagacg cgtcaagcgt cgcatcaggc
     6721 atggtgcacc gattgccgga tgctctcatc actacagcga aatacaggtc cccgcttcgc
     6781 ccgccagcgt ctcttcaatt cgcgataacg ccccaatcca cgcgggttta ctacggcttc
     6841 tgacataacc actcaccgcc gttaccttcg gccccatcga accatcggct ttggcaaatg
     6901 gcgctaactc atccggtgtg gcatggcgaa tggcacgttg ctgcggcgtt ccccagtttt
     6961 catataccgc atcagcatcg gtgaggatca ccagtccatc ggcattaatc tgctcggcga
     7021 gcaacgcagc ggcgagatct ttatcaatca ccgcttcact ccctgcccca tcttccgtca
     7081 caggcacacc gccgccgcca ctgcaaatca ccacatgccc ctctttgagc aacaactcga
     7141 tggcttcgct gtcgagaatt ttacgcggtt gcggagacgc caccacccgg cgcaaatatt
     7201 taccatcacg tttcatctgc cagccgtaag ccgcttccag tgcctcttgt tcttctggct
     7261 gataaaccgg accaataaat ttctctggct gcaaaaacgc cggatcatca ggcgaaacct
     7321 caatgcgcgt cagcaccgtc gtaacaggcg gcatctgcgg ctgtgcgctc aagctctgcg
     7381 ccagcatata gccaatcatc ccctggcttt ccgccaccag tacatccagc ggatacggtt
     7441 ctacctcttt ccacgccaga ttctgtaacg ccagcagccc cacctgcggc ccgttgccgt
     7501 gaacaatcgc cagccgataa gaacgggcca ggcgtgccag cgcgggtaca gcactggcga
     7561 tattgcgata ttgattttcc gccgtcagcg cctcaccgcg ctggagtaag gcgttgcccc
     7621 caagagctac aaccagtgtt ttcatggttt tccttgtaat aattgttggc caagccagaa
     7681 ccccagcagc gtatctgccc ctgacgtatg ccccagcgcc agcagcgaat caatcgcaac
     7741 agcggtacgt ttcgggcaac tcagagcatg gacaaagtgc aggagtggcg aggcgaaata
     7801 tccttgcgcg gcataacgta aataactgac gctcaccgct gtggtaacga gttgaagatt
     7861 gtcggaacag gcaaaaaacg ggcgaccggc gcgcgcatct aaagcgccat aataccaggc
     7921 cgccagcagc attccgctca acgtgtcatc atgacttggc gttaatccgg ggcctttacc
     7981 cagccagtgc cgccagtcgg tcttaacgcc attgagcgcg gcctgaaaac agtgacgaaa
     8041 ctggcgtaat tcagcgggca gcggatcgct tgccgccatc gccagtggcc cgaaaagtcc
     8101 ggtttcctcc gcgcgttgca tccatgcagc tgcaagcggt tgaggatgcg aaggcggcgt
     8161 aatacgcagc aaacaataac gctgtggctg tttaaccgtg aaacgcccga ggcgaatccc
     8221 ttgagccaca acctgtggtc gttcattgcc gcataatcca ccgcataacg catcgaattg
     8281 cgtgcggcga aattacacat tcccgtcaag ctatagaaga aacgctgccc tggctttcct
     8341 cttttggcgc tgacccaacg ggtgggatga cccgtttact ttattcgccg gaatggctgg
     8401 aaacccagca gcaatttaaa aaaagaatgg cagcaagtgg gctggaaaca cgtttcgatg
     8461 aagtggggaa tttatacggt cgcctgagtg gcaccgaata tccacaggaa gtggttctga
     8521 gcggttcgca tatcgatacc gtggttaacg gcggtaacct cgacgggcaa ttcggcgcgc
     8581 tggcggcgtg gctggcaatt gactggttga aaacgcaata cggcgcaccg ttacgtacgg
     8641 tcgaagtggt ggcgatggca gaagaagaag gcagccgctt cccgtatgtc ttctggggca
     8701 gtaaaaatat ctttgggctg gcgaatcctg acgacgtgcg gaatatctgt gatgccaaag
     8761 gaaatagttt tgccgatgcg atgaaggctt gcggatttac tctgccgaac gccccactaa
     8821 ctccgcgtca ggctataaaa gcctttgttg aactgcatat tgaacagggc tgtgtgctgg
     8881 aaagtaatgg gcaatcaatt ggcgtggtga atgcaattgt cgggcagcgt cgctatacgg
     8941 tgacgctgaa cggcgaatca aaccatgcag gcaccacgcc gatgggttat cgtcgtgata
     9001 cggtttacgc tttcagtcgc atttgccatc agtcgatcga aaaagcgaaa aagatgggcg
     9061 atccgctggt tctgaccttt ggcaaagtag agccgcgccc gaatacggta aatgtggtgc
     9121 cgggtaaaac cacgttcacc attgattgtc gtcataccga cgctgccgtg ctgcgcgatt
     9181 tcacccaaca gttagaaaac gacatgcggg cgatttgcga tgaaatggac attggtattg
     9241 atatcgattt atggatggac gaagaacccg tgccgatgaa taaggagctg gtcgccaccc
     9301 tgacagaatt gtgtgaaaga gaaaaactga attaccgggt gatgcacagt ggtgccgggc
     9361 acgacgcgca aattttcgcg cctcgcgtac caacctgcat gatttttatc cccagcatca
     9421 acgggatcag ccataacccg gcggaacgca ccaatattac tgaccttgcc gaaggggtca
     9481 aaacgttggc actcatgctt tatcaacttg cctggcagga ataaggagtc ataaatggga
     9541 tatttaaata acgtcaccgg ttaccgcgaa gatttactgg ctaaccgtgc gattgttaaa
     9601 cacggtaatt tcgcactgtt aaccccggat ggtctgataa aaaatattat tccggacttt
     9661 gaaaattgtg acgcgacaat cctctccacg ccaaagctgg gtgcctcttt tgttgattat
     9721 ctggtcacac tgcatcaaaa cggtggcaac caacagggct tcggtggcga aggcattgaa
     9781 acgttcctgt atgtgatctc tggaaatatc actgccaaag ccgaaggcaa aacatttgcc
     9841 ttaagcgaag gtggctatct ttattgccca ccaggctcct taatgacgtt tgttaacgcc
     9901 caggccgaag acagccaaat ctttttatat aagcgccgct atgttccggt agaaggccat
     9961 gcaccgtggc tgttttctgg caatgccagc gaactggaac gcattcacta tgaaggtatg
    10021 gatgatgtga tcctgctgga ttttctgccc aaagagttag gttttgatat gaacatgcat
    10081 atcctctctt ttgcaccagg tgccagccac ggttatatcg aaacacacgt tcaggaacat
    10141 ggtgcctata ttctttccgg tcagggggtt tata
//
LOCUS       DAZSIO010000141        10066 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZSIO010000141 DAZSIO010000000
VERSION     DAZSIO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.141"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            82..171
                     /locus_tag="ACHX5T_003194"
                     /pseudo
     CDS             82..171
                     /locus_tag="ACHX5T_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
     gene            473..1468
                     /gene="pgl"
                     /locus_tag="ACHX5T_003195"
     CDS             473..1468
                     /gene="pgl"
                     /locus_tag="ACHX5T_003195"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR8468273.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1509..2462)
                     /locus_tag="ACHX5T_003196"
                     /pseudo
     CDS             complement(1509..2462)
                     /locus_tag="ACHX5T_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2646..3698
                     /locus_tag="ACHX5T_003197"
     CDS             2646..3698
                     /locus_tag="ACHX5T_003197"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFR8468274.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3774..5207
                     /locus_tag="ACHX5T_003198"
     CDS             3774..5207
                     /locus_tag="ACHX5T_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFR8468275.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5390..7570
                     /locus_tag="ACHX5T_003199"
     CDS             5390..7570
                     /locus_tag="ACHX5T_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR8468276.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVEPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7711..8994)
                     /locus_tag="ACHX5T_003200"
     CDS             complement(7711..8994)
                     /locus_tag="ACHX5T_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR8468277.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(9129..>10066)
                     /locus_tag="ACHX5T_003201"
     CDS             complement(9129..>10066)
                     /locus_tag="ACHX5T_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8468278.1"
                     /translation="TEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILAS
                     RGIKQKTLINYMSKIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTL
                     SDAFREAIAEGHITTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMEL
                     AVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCK
                     EILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQ
                     ISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         2439 a         2354 c         2550 g         2723 t
ORIGIN      
        1 ctgtgtattc ccatggaagg agatcaccca attctccccc ccgacttttg acgcagtgtc
       61 ttgttaacag ggggattgac catgcttatt ggctatgtac gcgtatcaac aaatgaccag
      121 aataccgatc tacaacgtaa tgcgtttatt tgtgccggaa gtgagttgat tgttgcgcga
      181 atgggaagtt ggccagaagt tgttatggca tagtgtttac ggtcgttgat acaggtaaac
      241 aaaagcgggg agagtcatac tccctgaata gtcgcgaaaa tatctgtggc attgtcaaat
      301 tcgtgtcggt gactaatatt ttagtaatat gccattcgaa atggagtgct aacttattga
      361 tttaattgat gtgcgatatt gtattttatc gtgttttgtt gtctgttttt tatccagtaa
      421 gtgttgttat atgtatgaaa gataagggat ttatcatttt tgaggtgtgt tcatgaagca
      481 aacagtttat atcgccagcc ctgagagcca gcaaattcac gtctggaacc tgaatcatga
      541 aggcgcactg acgctgacac aggttgtcga tgtgccgggg caggtgcagc cgatggtggt
      601 cagcccggac aaacgttatc tctatgttgg tgttcgccct gagtttcgcg tcctggcgta
      661 tcgtattgcc ccggacgatg gcgcactgac ctttgccgca gagtctgtgc tgccgggtag
      721 tccgacgcat atttccaccg atcaccaggg gcagtttgtc tttgtaggtt cttacaatgc
      781 gggtaacgtg agcgtaacgc gtctggaaga tggcctgcca gtgggcgtcg tcgatgtagt
      841 cgaggggctg gacggttgcc attccgccaa tatctcaccg gacaaccgta cgctgtgggt
      901 tccggcatta aagcaggatc gcatttgcct gtttacggtc agcgatgatg gtcatctggt
      961 ggcgcaggac cctgcggaag tgactaccgt tgaaggggcc ggcccgcgtc atatggtatt
     1021 ccatccaaac gaacaatatg cgtattgcgt caatgagtta aacagctcag tggatgtctg
     1081 ggaactgaaa gatccgcacg gcaatatcga atgtgtccag acgctggata tgatgccgga
     1141 aaacttctcc gacacccgtt gggcggctga tattcatatc accccggatg gtcgccattt
     1201 atacgcctgc gaccgtaccg ccagtctgat taccgttttc agcgtttcgg aagatggcag
     1261 cgtgttgagt aaagaaggct tccagccaac ggaaacccag ccgcgcggct tcaatgttga
     1321 ttacagcggc aagtatctga ttgccgctgg gcaaaaatct caccacatct cggtatacga
     1381 aattgttggc gagcaggggc tactgcatga aaaaggccgc tatgcggtcg ggcagggacc
     1441 aatgtgggtg gtggttaacg cacactaacc gctgatttaa ccggcgcagt ctctcctgcg
     1501 ccggtgtatt aacttatctc ctgtaacgca tgtctctggc gttcgacgat attggtccac
     1561 aaattgtctt tatcgtcagt ccacaaatta atcagcaagg aaaaaaagcg ttcagctgcc
     1621 ggagaaagca cggcatcttt gcggcgaata atccccaatg tccgacgtat aacgggttcc
     1681 accagtggga taccaataat cgaagaatag ggcgcatggg gcattgccag accaggaagc
     1741 gccgaaatcc ccagtcctgc ctccactagt cctaatgacg tcgaaagatg acgcacttcg
     1801 taaaaccagt ccagcttcca gggcttgtcg gctagctgtt gttctatcaa caatcggttg
     1861 ccgctggagg aacgtacgcc aatcattttg taacccacta actcctgcca ttctacaagc
     1921 tactttttgg ccaatggatg gtcacgccga caggccagca cgaatggctc gttgaccagt
     1981 ggggtaaaat caatggatga atttgtgacg ttgttcatgt ttatgccaaa gtctgattcg
     2041 ttacacagca ccgattccat gcaattattc gtgccttgtt ccagaatccg cactttaata
     2101 ttgggataca gctcattaaa tttaccgatt gccagcggta aaaaataaaa tactgcggtc
     2161 ggaatacacg ctaatgtcac cataccacga tgataagcat tcatatcacg aatattaaaa
     2221 agcgtttcat cgaatttctt tattaattcc cgcgcctccg ggagcaacct ttttcctgct
     2281 tttgttaacg taaccttgcg agttgtacgc tcaaatagtt gaatatgtaa atcttcctcc
     2341 atctttttta ttcttcgcgt taatgcaggt tgcgtaatat tgagtaattt agcggcattg
     2401 ttaaatgaac tggactctgc cagtatgaca aatgccttca tacttgataa ttcatgcttc
     2461 attattactc cggaaaatgg aagcgacgat tttggggggc tggtcgttaa aaattttaac
     2521 tgcatttagc caacttaaat taatgaaaaa atgttattaa tcgttgagct aaagtcatta
     2581 gagatgcttt gtccttaatg taaccatatc gcaataagtt atgtttttaa attgagggca
     2641 ttattatgaa aaaaataccc tgcgtgatga tgcgaggtgg aacctcgagg ggcgcgttcc
     2701 tgttagcgga acatttaccc gaagatcaaa cgcagcgcga taaaatattg atggcaatta
     2761 tgggttccgg taacgatctg gaaattgacg gtattggcgg cggtaatcca ctgaccagta
     2821 aagtcgccat tattagccgt tccagcgatc cgcgtgctga tgtcgattat ctgtttgctc
     2881 aagttatcgt ccatgagcaa cgtgtcgata ccacgcctaa ctgcggcaat atgctgtctg
     2941 gcgtaggggc attcgccatt gaaaatggtt tgattgcagc gacttcgcca gttacccgtg
     3001 tacgtatccg caacgtcaat acgggtacgt tcatcgaagc tgatgtgcaa acgccaaatg
     3061 gtgttgtcga gtacgagggt agcgccagaa ttgacggcgt accgggtact gccgcaccgg
     3121 ttgcgctcac tttcctgaat gccgctggaa cgaaaactgg aaaagttttc ccgactgata
     3181 atcagattga ttattttgac gatgtcccgg tgacctgtat cgatatggcg atgccagtcg
     3241 tcattattcc ggctgaatat ctgggaaaaa caggttatga attaccggcg gaactggatg
     3301 ccgacaaagc attattagcc cgcattgaat ctatccggct acaagcgggt aaagcaatgg
     3361 gcttaggtga tgtcagtaat atggttatcc ctaaacctgt gcttatttct ccagcgcaga
     3421 aaggcggggc aattaatgtg cgttatttta tgccgcattc ttgccatcgc gcgctggcga
     3481 taaccggtgc tattgctatt tccagtagtt gtgcattgga aggcaccgtc acccgacaaa
     3541 tcgtcccttc tgtaggatat ggcaatatca atattgaaca ccccagtggt gcgctcgacg
     3601 ttcatttaag taatgaaggt caggatgcca cgacgttacg cgcatctgtt attcggacaa
     3661 ccagaaaaat attttccggt gaagtttatc ttccctgaaa aaattcgttg tcaggataag
     3721 gacaatcaat aaaggacttc tgtatgagtc atacagaaag aacaggattt taaatgaata
     3781 agaaatcgtt atggaagcta attctgatat tagcgatccc atgtattatt ggttttatgc
     3841 cagctccggc aggattaagc gaactggcgt gggtgctttt tggtatttac ctggcggcca
     3901 ttgtgggtct ggttatcaag cctttcccgg aacctgtcgt actgttaatt gccgttgctg
     3961 cctcaatggt ggtggtcggt aacttatccg acggtgcgtt taagaccacc gccgtattaa
     4021 gcggttactc ttcaggtact acctggctgg tgttctcggc gtttacctta agcgccgcat
     4081 ttgtgaccac cggtttaggt aaacttattg cctatctgct gattggtaaa atcggtaaca
     4141 ccatgctggg tctgggttac gttacggtat tcctcgatct ggtactggct ccggcaacac
     4201 cgtctaacac cgcgcgtgcg ggcggcattg tgttaccgat catcaacagc gtggcggtgg
     4261 ctttggggtc cgaaccggaa aaaagtccgc gtcgtgtcgg acattacctg atgatgtcca
     4321 tttacatggt caccaaaacc accagctata tgttctttac cgcaatggcg gggaacattc
     4381 tggcgctgaa aatgatcaac gacattctgc acctgcaaat tagctggggt ggatgggcgc
     4441 tggccgccgg attgccgggc atcattatgc tgctggtcac cccgctggta atttacacca
     4501 tgtatccacc agaaattaaa aaggtggata acaaaaccat cgctaaagcg ggccttgccg
     4561 aactgggacc gatgaaaatc cgcgaaaaaa tgctgctcgg tgtctttgtg ctggcgctac
     4621 tgggctggat tttcagtaag tctctggggg ttgatgaatc cactgtggca atcgttgtta
     4681 tggcgaccat gctgctgctg ggtatcgtta cctgggaaga cgtggttaaa aataaaggcg
     4741 gctggaatac cttaatctgg tacggcggta ttatcggctt aagctcctta ttatcgaaag
     4801 ttaaattttt cgaatggtta gctgaagtct ttaaaaataa cctggcattt gatggtcacg
     4861 gtaacgttgc tttcttcgtt attattttcc tcagcatcat cgtgcgttat ttcttcgctt
     4921 ccggtagtgc ctatatcgtt gctatgttac ctgtatttgc catgctggcg aacgtctccg
     4981 gcgcaccgtt aatgttaacc gcgctggcac tgttgttctc caactcctat ggcggcatgg
     5041 ttactcacta tggcggcgcg gcaggtccgg tcatctttgg cgtgggttac aacgatatta
     5101 aatcctggtg gttggtcggt gcggtactga cgatattaac cttcttggtg catatcaccc
     5161 tcggcgtgtg gtggtggaat atgctgatcg gctggaacat gctgtaaata tactcgtcat
     5221 acttcaagtt gcatgtgctg cgtctgcgtt cgctcacccc agtcacttac ttatgtaagc
     5281 tcctggggat tcactcactt gtcgccttcc tgcaactcga attatttaga gtatatccat
     5341 ttattatctt tctgcgcact tcacggtgcg cagatatctg gagcatttga tgatcaagtt
     5401 atctgaaaaa ggcgtgtttc tcgccagtaa taacgaaata attgccgaag aacatttcac
     5461 cggcgaaatt aaaaaagaag aagcaaaaaa aggcactatt gcctggtcta ttctctcttc
     5521 tcataatacg tccggaaata tggataaact taaaattaag tttgattcat tagcctctca
     5581 cgatattacc tttgttggta ttgtacagac cgctaaagcg tccggaatgg aacgtttccc
     5641 gctgccgtat gtgctgacca actgccataa ctcactctgc gccgtcggcg gcaccattaa
     5701 cggtgatgac catgtttttg gtttatcggc agctcagcgt tatggcggta tttttgtgcc
     5761 tccgcatatt gcggtcatcc atcaatatat gcgtgagatg atggctggcg ggggcaaaat
     5821 gatcctcggg tcagacagcc acacccgtta cggtgcatta gggacaatgg cagtcggtga
     5881 gggtggcggt gagttggtaa aacagctgct taatgacacc tgggatatcg actatccggg
     5941 cgtggttgcg gtgcatctga ccggaaaacc tgcgccgtat gtggagccac aggatgtggc
     6001 gctggctatc attggcgcgg tgttcaaaaa cggttacgtc aaaaacaaag tcatggagtt
     6061 cgttggaccg ggcgttagcg cgctctctac cgatttccgt aacagcgttg acgtgatgac
     6121 cacggaaacc acctgtttaa gctccgtctg gcaaactgat gaagaagtgc ataactggct
     6181 ggcgctgcac ggtcgcggtc aggattactg ccagcttaac cctcaaccga tggcgtacta
     6241 cgatggctgc atcagcgttg atttaagcgc catcaaacca atgattgcgc tgccgttcca
     6301 cccgagcaac gtgtatgaaa tcgacacact gaaccagaac ctgaccgaca ttctgcgtga
     6361 gattgaaatt gagtccgaac gcgtggcgca cggtaaagcc aaactctcgc tgctggataa
     6421 agtggaaaat ggtcgcctga aagtgcagca ggggattatc gcgggctgtt ctggcggtaa
     6481 ctacgaaaac gtcatcgcgg cggcgaatgc actgcgcggt caatcctgtg gcaatgacac
     6541 cttctcgctg gcggtttacc cgtcatcaca gccggtgttt atggatctcg ccaaaaaagg
     6601 tgtggtagca gatttgattg gcgcaggcgc aatcatcaga accgcattct gtggcccatg
     6661 ctttggcgcg ggcgatacgc caatcaacaa cggtttgagt attcgccaca ccacgcgtaa
     6721 cttcccgaac cgcgaaggct ctaagccagc caatgggcag atgtcagcgg tggcgttgat
     6781 ggacgctcgt tctatcgctg cgactgcggc aaacggtggc tatttaacct ctgccagcga
     6841 acttgattgc tgggacaacg tgccggagta cgccttcgat gtaacgccgt ataaaaaccg
     6901 tgtttatcag ggctttgtga aaggggcaac tcagcaaccg ctgatttacg gaccgaacat
     6961 taaagactgg ccggaattgg gtgcgctgac tgacaatatc gtcctgaaag tgtgctcgaa
     7021 gatcctcgac gaagtcacca ccaccgacga actgattcct tccggtgaaa cctcttctta
     7081 tcgttcaaat ccgattggtc tggcggggaa tgtcagcgag ctgacagagg tgtttgcgcg
     7141 cattaagcag attgctggtc aggagcatat tgatccgctg caaactgaaa ttggtagcat
     7201 ggtctatgcg gtgaaaccag gcgatggttc tgcgcgtgaa caggcggcga gctgccagcg
     7261 tgtgattggc ggtctggcga atattgccga ggagtacgcg actaaacgct accgttctaa
     7321 cgtcatcaac tgggggatgt taccgctgca gatggcggaa gtgccaacct ttgaagtggg
     7381 ggattacatt tacatccctg gcattaaagc ggcgctggat aatccgggta cgacgtttaa
     7441 aggttatgtg atccatgaag atgcgccggt aacggaaatt acgctttaca tggaaagcct
     7501 gacggctgaa gagcgcgaga ttatcaaggc gggtagtttg attaacttca ataaaaaccg
     7561 tcagatgtaa aaagcgccat gtgaatgtag gtcgcattcg gcacttattg tcggatgcga
     7621 tgctggcgca tcttatccga cctacaaatc gcatcgaacc gtaggccgga taaggcgttt
     7681 acgccgcatc cggcaaatag ttaatagctc ttacttcttc tcctctgcaa ccactttgct
     7741 gcccacgccg cggttattgt attcccacat gcggttgtag ttagtgtcat tcagattgcg
     7801 ctgtatttcg tcgttatcat ctacgctgcc ggtattaccc gcaaacggac gattagagat
     7861 caccgcatcg gcccacggtt tagccgtgtt aaaaccttcg ttgatggcgc tatcacggat
     7921 caccacctga ccgttggtat tagcatcaac atccagcgag cggcccagtt gcgccacgcc
     7981 atcaccggaa gcattgaaac ggctgtttac ggcgaggaaa ccgtaataga tgttagacag
     8041 cgtagccggt gcaaacacat atgcttcttg ctgggtacgt gagttcacca cgcggaattc
     8101 ggtgttatcg aacaccactg cgccgcgacc agaaacgata tccacatccc cttcaatata
     8161 gctgttggtc accagcgtac gcggctgacg gttggtttcc agacggttct gcacaccgct
     8221 gttggtgaca aagaaggtgt tctgacgacc gagaatgtta acgttgttaa tctgtacttg
     8281 gtcaccgtca gtacgcagtg ctaccgccgg atggttacct gcatctacgc tatcgcccag
     8341 cgtgttttcg atggtcagat tttgcagttg cagtccattg ttttgtgacc agaagaccgc
     8401 agaacacaga acaccgatac tgtcgctgcg tttgctctgg cagctatcgt acatatacca
     8461 cgctggttta cctggcatat atttgccgcg cgggttgacg tcgtgacgcc agtcggcagg
     8521 gctcatgccc ccatcaaggg aaagcccaat cttcacatca atcggttttt cacctgtacc
     8581 gtacagagtg attccacctg gagcagcagg gacatacacc gttccctgat actcaccagg
     8641 catcacggca atatactggc gcttgttggt gcgcttgata attgccgcat ctaccgccgc
     8701 ctgaatcgtg gtatgcgtta caccttgagt acccgccggg ccgacaacaa agtcaggttg
     8761 cgcaggcagg gttatcgggg acggattcca cgctgcagca cctggtgtca gggatgcaaa
     8821 atagtgttgc gcatcgaaat tcagcgcttc ttttgccgac agaatcgggc gagaagaggt
     8881 accaggcgca gtttgatcag aaggacgttg atcgggtggt gttgagctac aggcggtcag
     8941 cgtcacgcca aaagccaatg ccagcgccag acgggaaact gaaaatgtgt tcacaggttg
     9001 ctccgggcta tgaaatagaa aaatgaatcc gttgaagcct gcttttttat actaagttgg
     9061 cattataaaa aagcattgct tatcaatttg ttgcaacgaa caggtcatta tcagtcaaaa
     9121 taaaatcatt atttgatttc aattttgtcc cactccctgc ctctgtcatc acgatactgt
     9181 gatgccatgg tgtccgactt gtgcccgaga agatgttgag caaacttatc gcttatctgc
     9241 ttctcataga gtcttgcaga caaactgcgc aactcgtgaa aggtaggtgg atccccttcg
     9301 aaggaaagac ctgatgcttt tcgtgcgcgc ataaaatacc ttgatactgt gccggatgaa
     9361 agcggttcac gacgagtaga tgcaattatg gtttctccgc caagaatctc tttgcattta
     9421 tcaagtgttt ccttcattga tatcccgaga gcatcaacat gcaatgttgt agggatggca
     9481 atttttacgc ctgttttgct ttgctcgaca taaagatatc catctacgat atcagaccac
     9541 ttcatttcgc ataaatcacc aactcgttgc ccggtaacaa cagccagttc cattgcaagt
     9601 ctgagccaac atggtgatga ttctgctgct tgataaattt tcaggtattc gtcagccgta
     9661 agtcttgatc tccttacctc tgattttgct gcgcgagtgg cagcgaccgg gtttgttgtt
     9721 atatggcctt cagctattgc ctctcggaat gcatcgctca gtgttgatct gattaacttg
     9781 gctgatgccg ccttgccctc gtctatgtat ccattgagca ttgccgcaat ttcttttgtg
     9841 gtgatgtctt caagtggagc atcaggcagc cccctcctta ttgctttaat tttgctcatg
     9901 taatttatga gtgtcttctg cttgattcct ctgctggcga ggattttttc gtagcgatca
     9961 agccatgaat gtaacgtaac agaattatca ctgttgattc tcgctgtcag aggcttgtgt
    10021 ttgtgtcctg aaaataactc aatgttggcc tgtatagctt cagtga
//
LOCUS       DAZSIO010000142         9937 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZSIO010000142 DAZSIO010000000
VERSION     DAZSIO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.142"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="ACHX5T_003202"
     CDS             complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="ACHX5T_003202"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR8468279.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="ACHX5T_003203"
     CDS             complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="ACHX5T_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR8468280.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="ACHX5T_003204"
     CDS             complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="ACHX5T_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR8468281.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(4161..4886)
                     /gene="phoU"
                     /locus_tag="ACHX5T_003205"
     CDS             complement(4161..4886)
                     /gene="phoU"
                     /locus_tag="ACHX5T_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR8468282.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4901..5674)
                     /gene="pstB"
                     /locus_tag="ACHX5T_003206"
     CDS             complement(4901..5674)
                     /gene="pstB"
                     /locus_tag="ACHX5T_003206"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR8468283.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5766..6656)
                     /gene="pstA"
                     /locus_tag="ACHX5T_003207"
     CDS             complement(5766..6656)
                     /gene="pstA"
                     /locus_tag="ACHX5T_003207"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR8468284.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6656..7615)
                     /gene="pstC"
                     /locus_tag="ACHX5T_003208"
     CDS             complement(6656..7615)
                     /gene="pstC"
                     /locus_tag="ACHX5T_003208"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR8468285.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7702..8742)
                     /gene="pstS"
                     /locus_tag="ACHX5T_003209"
     CDS             complement(7702..8742)
                     /gene="pstS"
                     /locus_tag="ACHX5T_003209"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR8468286.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8990..>9937)
                     /gene="lpfD-O113"
                     /locus_tag="ACHX5T_003210"
     CDS             complement(8990..>9937)
                     /gene="lpfD-O113"
                     /locus_tag="ACHX5T_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR8468287.1"
                     /translation="SGYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIY
                     YTSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKY
                     NCNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMAD
                     VYIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLG
                     ILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTV
                     YGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         2563 a         2587 c         2295 g         2492 t
ORIGIN      
        1 tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat tggggatcat
       61 attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa cacaaccagt
      121 catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg cgtcactttc
      181 agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc cgggatattg
      241 gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca tggcctgcaa
      301 catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca tattgccgat
      361 tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg caaccagttg
      421 atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc ccacgcccgt
      481 gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg ctactttatg
      541 ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga ccgcccgatt
      601 agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg ataacgttac
      661 cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa acgctaaccc
      721 cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg caatggaaat
      781 acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt aacggtaata
      841 aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt ccatttttcc
      901 cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt ccagccaggc
      961 accttccacc tgattggcag ctgttgcgcc accccaaaga aatgtttctg gaaatgcttt
     1021 cataattaac tccttttatc gttagcgaat gatggataac agcggttcac ctgcgcttat
     1081 ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga ttaataccgg
     1141 cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa tcagccgatc
     1201 gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc cgtccagttt
     1261 tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact caatgccaat
     1321 ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac gcacttcacc
     1381 aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg tggtatcagc
     1441 gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc cctgctcagg
     1501 tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct gacgtttact
     1561 ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa tgacaccgcc
     1621 aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct gcatgaaggt
     1681 gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa tgatggtggc
     1741 ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca ggttgacgcc
     1801 atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc ccgccaccac
     1861 ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga cctgcgccat
     1921 aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga agttattgat
     1981 acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc agaagccgcc
     2041 cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac cggcggcaat
     2101 catttcgctt atccaggttg atagctgccc caccagcaga aaggtgacgg gtgtgataac
     2161 catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc acgcattaag
     2221 tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa ccgatgacga
     2281 gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat ccgctttttg
     2341 cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa tcaccatggc
     2401 agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta tcgggaagaa
     2461 cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt gttcggtcgt
     2521 ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg ccgccatcag
     2581 accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta gcagattacc
     2641 tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca cgctgttaac
     2701 cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac caccgctttc
     2761 caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt tgctttcatc
     2821 ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt tatctgcgcc
     2881 cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc taccgctttg
     2941 cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa gcaatttttt
     3001 tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata tttcagtgtt
     3061 ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt gaaattttac
     3121 gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat gcttgcgggt
     3181 aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa gcatgttcaa
     3241 gaatacgcca ggataaaaac ttcagatgtg taaccagtcg ctgataactc aagctttcct
     3301 cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc attaactgcg
     3361 tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga tgcatggcaa
     3421 taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata atggttaacg
     3481 cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac agcaacacgt
     3541 tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata taaatactgt
     3601 cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag gttgccatca
     3661 cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca tgactgctca
     3721 aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt ttttgaaagc
     3781 caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc accacaacat
     3841 tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt ataaacaaaa
     3901 aaaaaccgac ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga atgcagtaac
     3961 aatccagtca tttattaatg gtttttataa cgaacatcca ggttcggaaa ttaatttaat
     4021 tgcgtgcaga gaaaatagca atgcgctatt gataaaaata tgaccatgct cgcagttatt
     4081 aactttgtgt aattttagga atttataaag ttatatataa caaatcccaa taattaagtt
     4141 attgggattt gtctggtgaa ttatttgtcg ctatcttttc ccgccagcag tttatccagc
     4201 tcatcaccgc ctacatgacg gaaatcctga cccttcacgt agtagaagat aaactcacaa
     4261 atattctggc agcggtcgcc gatacgttcg attgaacgcg cacagaacag cgcggtaagt
     4321 acgctcggaa tagtgcgcga atcttccatc atgtaggtca tcagttgacg aacaatacct
     4381 tcgtattcct gatcgacttt tttatcttca cgataaatac gtaccgcttc gtcaatgtcc
     4441 atccgcgcga acgcgtccag cacgtcgtgc agcatctgga tggtatgacg gcccagcgac
     4501 tccagactta ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc agtacggcag
     4561 attttgtccg ccacgtcgcc aatacgctcc agctcggcaa tggttttact gatcaccata
     4621 accagtcgca ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac gcaggcttca
     4681 tcgatcgcca cttccatcat gttgacgttc ttgtcgcctt cgatcacgcg cttcgccaga
     4741 tcgctgtcct ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc caccatgccg
     4801 cccatggtca tcacctgcgt gcggatactt tccagttcgg cgttgaactg gccggaaata
     4861 tgtttattaa gattgagact gtccataacg cactcctgaa tcaaccgtaa cgaccggtga
     4921 tgtagtcttc tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg ttgctgaact
     4981 caatcaattc gcccaggtac ataaatgccg tgtggtcgga acaacgcgca gcctgctgca
     5041 tgttgtgggt gacgatcacc acggtgtaat cctgcttcag ttcggtgatc agctcttcaa
     5101 tacgcccggt agagatgggg tcgagcgccg aacacggttc gtcgagcagc agcacttccg
     5161 ggcgaatggc gataccacgc gcaatacaca gacgctgttg ctgaccacca gagagagagt
     5221 aaccgctctg gtgcaattta tctttggttt cgttccacaa tgcggctttg gtcaatgccc
     5281 actgcacgcg ctcgtccatg tcggcacggg agagcttctc aaacagacga acgccaaagg
     5341 cgatgttgtc gtagatggac atcgggaacg gcgtcggttt ctggaacacc atgcccactt
     5401 tcgcacgcag cagggcgata tcctgagagt tggtcaggat gttgtcgcca tcaagcagaa
     5461 tttcaccttc cgcacgctgc tccgggtaca gttcaaacat tttgttgaag gtacgcagca
     5521 gcgtcgattt accgcagccg gacggcccga taaacgccgt aacctggttt ttggcgatat
     5581 ccaggttgat gtttttcagg gcatggaatt tgccgtagta gaagttcaaa ttacgaacct
     5641 gaattttact cggggcagtt tcaaccatac tcattgcaat cttttcctca tcgtacctga
     5701 tgcttttcgc taatcaggct taccgtaggg ccgggtaagg cgttcacgcc gcatccggaa
     5761 aagtgttaac cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt cagcagcagg
     5821 acgcacaggg taatgatcaa taccccggcc caggccagtt gttgccattc cgcaaacggg
     5881 ctcatcgcaa acttaaagat cgtcaccggc aggttggcga tcggctgcat catgtccgtg
     5941 ctccagaact ggttggagag cgcggtaaac agcagcggcg cggtttcacc ggcaatacgg
     6001 gcaatcgcca gcaggatacc ggtcataatc ccggacaccg acgctttcag cgtaatcgcg
     6061 gagatcatct tccacttcgg tgtacccagc gcataagccg cttcacgcag gctgtccggc
     6121 accagtttca gcatgttctc agtggtgcgg ataacgatcg gcacctgcaa cagcgccagc
     6181 gcaattacgc ccgcccagcc ggagaagtgc tccatctgtg ccaccacgat ggtgtagaca
     6241 aacagaccaa ccacaatcga cggcgcggag agcagaatgt cgttaatgaa gcgaatcact
     6301 tctgccagcc aggatttacg accatattcc gccagataaa tccccgccat aatgcccagc
     6361 ggcgtaccga ataccgtggc ccacaaaatt aacagcccgc tacccgccag ggcgttcgcc
     6421 agaccaccac cttccgtatt gggcggcggc gtcatttcag tgaacagcgc cagcgacata
     6481 ccgtcgatac cgcgagtgat ggtggacatt aaaatccaga tcagccagaa caggccgaag
     6541 gccatcgtcg ccatcgagag cgtcagcgca atacggtttt tgaggcggcg acgcgcctgc
     6601 attttgcggc gagattcagc cagcgccgca gtggtttgca tttcaaccat agccattagc
     6661 gtgccccctc attcttagcc aggcgcataa tcataaactt cgatgcggcg aggacgatga
     6721 aggtaatcac aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc agaccggatt
     6781 ccgcttccgc aaattcgttc gccagcgcag aggtgatact attgcccggc atgtacagcg
     6841 aggcgctgtc gagctggtag gtgttaccga tgataaaggt caccgccatg gtttcaccga
     6901 gcgcgcggcc cagccccagc atgatgccgc cgataacacc atttttggtg aacggaagaa
     6961 cgatacgcca gataacttcc caggtggtgc agccaatacc gtaggccgac tctttcatca
     7021 tcaccggggt ttgttcgaac acatcacgca ttaccgccgc aatgtacgga ataatcatga
     7081 tggcgaggat cacgcctgcc gcgaggatac cgataccaaa tgcggggcca gagaacagcg
     7141 cgccaacaat cgggatattc gacatgatat tgccgaccgg ctcctgaaag taaacggcga
     7201 acagcggcgc aaagataaac aggccccaca tgccgtaaac gatacttgga atggctgcca
     7261 gcagctcaat ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc tcagtcagga
     7321 acagggcgat accgaaactc accgggacgg cgatcagcag cgcgataaac gaagtcacca
     7381 acgtaccgta gatcggcacc agcgccccgt agatatcgtt cggtgcatcc cactctttgg
     7441 tccataggaa agccagacca aatttctgaa tgctcggcca ggaggagatg atcagagaga
     7501 caataatgcc acccaacatc aatagcacaa tcagcgccgc cagttttacc agcacgctga
     7561 aaattatgtc gcccttttta cccggtgggt taaaagcggg cttggttgca gccataagtt
     7621 actcttcagt taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg cgtaaaacac
     7681 cgtacccggc ctggagtttt attagtacag cggcttaccg ctactgtctt taatattggt
     7741 cttccacgca gcgcgaacct gttcaactac actatccggc aggctggcgt aatccaggtc
     7801 gttcgcctgt ttagccccgg ttttgtacgc ccagtcgaag aatttcagca cctctgtgcc
     7861 ttgttctggt ttcttctgat ctttgtggat cagaatgaac gtggtagagg taataggcca
     7921 tgcatcttcg cctttctggt tggtcagatc ctgagcgaag gttttgctcc agtctgcacc
     7981 ttttgctgca ttagcgaagt tttcttcggt cggactaacc ggtttaccat cagcggagat
     8041 cagtttggtg tacgccaggt tgttctgctt cgcgtaagca tattcaacat aaccaattgc
     8101 acccggcaga cgctgaacga acgcggcgat accgtcgtta cctttaccgc ccagaccgat
     8161 cggccatttt acggtagagc cagtaccaac gttgtttttc cactcttcgt tcactttcgc
     8221 caggtagctg gtgaagacga aggaagtccc ggagccatct gcgcggcgta ctacagcaat
     8281 gttttgtgaa ggcagtttca gacccggatt cagtttggcg atggcttcat catcccactt
     8341 cttgattttg cccaggtaga tgtcgccgag ggttttacca tccagcacca gttcgccaga
     8401 cttcagccct ggaatgttaa ccgccagcac cacgccgcca atcacggtcg ggaactggaa
     8461 cagaccttcc tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac caaaatcaac
     8521 ggtattagcg ataatctgtt ttacgccacc ggaagaaccg ataccctggt agttaacttt
     8581 attaccggtt tctttctggt aagtgtcagc ccatttggca tacaccggcg cagggaaggt
     8641 tgcacctgca cctgtcaggc ttgcttctgc aaacacagag aaagcactca tcgataaggt
     8701 cgcggcgaca acagttgcga cggtggtacg cataactttc ataatgtctc ctgggaggat
     8761 tcataaagca ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt gacagttata
     8821 tataaggaat atgacatttt tatgacagaa aggaattacc aaataactta ctgattttaa
     8881 atgtgaagat gaaaattcca catgccatct ttttgtcatg atgcatttaa aagtgataaa
     8941 gaaatgggtg agatgacaac gatgtgatga acaatcgttg tcagacctat tagcgcacaa
     9001 tctcaacagt tatcgtcgct gttgcagtaa attgccccgg ggctgggcga gctccagtaa
     9061 ggctcgcagg agcagctgaa aaagttacac tacctgattt ttcaccatac actgttgatt
     9121 tatccatgtc tgttggcaac tcaccaccgt tcactttgat ttccttatta tttttgtcgt
     9181 aaatttttat ccccacgtta gagtttgaag ttacgaccat tgaattgtcc gtattgggtt
     9241 cggtagcata aaaagacgta ctgatatcat cggtattcag tatccccagg ttttcacact
     9301 caatagtgac tgttttagta attttatgtg ttgttaccgc acttccaaca acagatgaaa
     9361 aatctgcagc tggtatttct ttaaaatcaa aacaaactgg ctttaaatta tttatttcgc
     9421 aactttgcgg agctgtaata tcgccccgaa tataaacatc agccatcggc tgtgagtaat
     9481 ccaccggggt gtcacggcta attgttccat acaatgtcac tatctttgtt tcaggaataa
     9541 tcaattttcc tgcaaatgtc ttcttgatat aaaaatatac tatcgcttta gccccactgg
     9601 atatacttaa aggttcaatg cggttgcaat tgtactttgt attaccagac gggttgttag
     9661 catgatattc aaaaggtgca ttcacatacc caaccccaag aatatctata gcaatcccaa
     9721 catcaaggtt cggattaaga tcataataat taatattaga gtgaacccca gcataagtta
     9781 atgcctggtt tatttttgac gtataataga tataggggaa ttctcctacg ttaccagtat
     9841 cacaatgaca gcgtccaaga tatgaggatc cattagttac agtggtgtta aatgtcgttc
     9901 caaccttatt ttcgttcgcg gtaataagat atcccga
//
LOCUS       DAZSIO010000143         9774 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZSIO010000143 DAZSIO010000000
VERSION     DAZSIO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.143"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            392..2023
                     /gene="oppA"
                     /locus_tag="ACHX5T_003211"
     CDS             392..2023
                     /gene="oppA"
                     /locus_tag="ACHX5T_003211"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR8468288.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2109..3029
                     /gene="oppB"
                     /locus_tag="ACHX5T_003212"
     CDS             2109..3029
                     /gene="oppB"
                     /locus_tag="ACHX5T_003212"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR8468289.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3044..3952
                     /gene="oppC"
                     /locus_tag="ACHX5T_003213"
     CDS             3044..3952
                     /gene="oppC"
                     /locus_tag="ACHX5T_003213"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR8468290.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3964..4977
                     /gene="oppD"
                     /locus_tag="ACHX5T_003214"
     CDS             3964..4977
                     /gene="oppD"
                     /locus_tag="ACHX5T_003214"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR8468291.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4974..5978
                     /gene="oppF"
                     /locus_tag="ACHX5T_003215"
     CDS             4974..5978
                     /gene="oppF"
                     /locus_tag="ACHX5T_003215"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR8468292.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6031..6360)
                     /locus_tag="ACHX5T_003216"
     CDS             complement(6031..6360)
                     /locus_tag="ACHX5T_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR8468293.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6395..7855)
                     /gene="cls"
                     /locus_tag="ACHX5T_003217"
     CDS             complement(6395..7855)
                     /gene="cls"
                     /locus_tag="ACHX5T_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR8468294.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7998..8171
                     /locus_tag="ACHX5T_003218"
     CDS             7998..8171
                     /locus_tag="ACHX5T_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR8468295.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8226..9479)
                     /gene="kch"
                     /locus_tag="ACHX5T_003219"
     CDS             complement(8226..9479)
                     /gene="kch"
                     /locus_tag="ACHX5T_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR8468296.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2563 a         2286 c         2433 g         2492 t
ORIGIN      
        1 tgaaagatgt ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga
       61 gaatcgctta acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc
      121 gggcaaccgc cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg
      181 cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc
      241 tgaatcacca gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca
      301 gcagaaagtc tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac
      361 cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt
      421 agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc
      481 cgcaggcgtc acactggcgg aaaaacaaac actggtacgt aacaatggtt cagaagttca
      541 gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt
      601 tgaaggctta ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg
      661 ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga
      721 tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa
      781 cactgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat
      841 tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt
      901 agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac
      961 ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa
     1021 tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct
     1081 tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt
     1141 gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac
     1201 gtataacaac atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt
     1261 tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga aaccgccatt
     1321 caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata tcattgttaa
     1381 taaagtgaaa gcgcagggcg acatgcccgc ctatggttat actccaccgt atactgatgg
     1441 cgcaaaattg actcagccag aatggtttgg ctggagccag gaaaaacgta acgaagaagc
     1501 gaaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgcg
     1561 gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa
     1621 aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg
     1681 tcaccagggc acttttgatg tggcccgtgc aggctggtgt gcagactaca acgaaccaac
     1741 ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc
     1801 ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc
     1861 tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta
     1921 ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca aagatccgct
     1981 ggataatacc tatacccgga atatgtacat tgtgaagcac taatggcaat acgtggggca
     2041 ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggcac aggccagaag
     2101 gtagggcaat gttaaaattt attctacgtc gttgtctgga agcgattccg acgctattta
     2161 ttcttattac tatttcgttc tttatgatgc gcctcgcacc gggaagccct tttaccggcg
     2221 aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat cttaatgatc
     2281 cgatcatgac acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat
     2341 cgtttaaata taaagattat tcggtcaatg acctggtggc atccagtttt cccgtttctg
     2401 ccaaactggg agccgccgca tttttccttg cggtaatact gggtgttagt gctggcgtta
     2461 ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg
     2521 gggttgttat ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt
     2581 tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga
     2641 tggtggcgtt gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga
     2701 ttgaagtatt acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc
     2761 ggatcatttt acgccatgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc
     2821 ccgcatttgt cggcattatt accggttcta tggtcatcga aaccatttat ggtttgccgg
     2881 ggattgggca actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc
     2941 tgaccatcct ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg
     3001 cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa
     3061 aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg
     3121 gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct
     3181 ggtgctgatc gcgttatttg taatcctggc accgatgctt tcccagtttg cctatgacga
     3241 tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac
     3301 tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat
     3361 ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc
     3421 cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa tcctcaactc
     3481 cttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggccaaa acatcctgct
     3541 gattttcgtg gcgattggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca
     3601 aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac
     3661 gccgggcatt gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc
     3721 atcgctactg gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg
     3781 tacgcaagag ccgttaagca gctggggggc attgctgagt gatggcgcga actcgatgga
     3841 agtctctcca tggttactgt tgttcccagc gggattcctt gtggtgacac tgttttgttt
     3901 caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca
     3961 gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg
     4021 ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc
     4081 aatgatttaa atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt
     4141 tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt
     4201 ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat
     4261 aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc
     4321 tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa
     4381 gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt
     4441 aaacgcatga aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agtcatgatt
     4501 gcgatggcat tgctatgtcg acctaagttg ctgattgcgg atgaacccac tacagcgctg
     4561 gacgtcaccg tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat
     4621 actgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg
     4681 ctggtaatgt acgccgggcg cacgatggaa tatggcaatg cgcgcgatgt cttttatcaa
     4741 cccgttcatc cttattctat cggtttgctc aacgcggtgc cgcgtcttga tgcggaaggt
     4801 gaaacaatgt tgaccatccc gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc
     4861 ccgttccagc cacgttgtcc acatgcgatg gaaatttgta gtagcgcacc gccgctggaa
     4921 gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg
     4981 ctgtaactga aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg cactttgaaa
     5041 tcaaagatgg caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg
     5101 tcactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta
     5161 agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt catgttgcct
     5221 ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata
     5281 ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga
     5341 tcatcgctga accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc
     5401 gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc
     5461 atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt attcttgaac
     5521 cgaagctgat tatctgcgat gagccggttt cggcgctgga cgtgtcaatt caggcgcagg
     5581 tggttaacct gctccagcag ctgcaacgtg agatgggatt gtcattaatt tttattgctc
     5641 atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg
     5701 cagtagaact ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg
     5761 cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt
     5821 tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc
     5881 gttgcccgat tgccggtccg gagtgcgcca aaacacgtcc tgttctggag gggagtttca
     5941 gacacgccgt ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg ctgacagttg
     6001 tcagcccttt ttcacgctaa aagcgattat ttattcccgc cagatgatat ggcaaagttt
     6061 gtgatctttt tcgcggcata acagaatgcg ggcgaaaaca tcgctgattt caccatcttc
     6121 actgtccgcc agaccaatca ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac
     6181 atgttcctgc cagtcttccg ccggatcgaa taactccgcg ccgccgcgct cttcaaactg
     6241 aagattgaac agcagaacat cagctggatc gagattgtcg gcagccagtt cgagaaaaat
     6301 atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga ttgtttagat ccatatccat
     6361 agtcactacc tgtttaacct ctgttggcga cgttttacag caacgggctg aagaagtaaa
     6421 acagtcgctc ggcgacacgt tgccataatg gacgttttag ccataaacgg gcatcgagca
     6481 gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca aaacctttat
     6541 cgtcgattgc caaggtaatc tcgaaattca gccacagact acgcatatca aggttaacgg
     6601 tgccaaccag acttagttcg ccatcgacca gcacgctctt ggtatgcagt aacccgcctt
     6661 caaactgata aattttaacc ccagcagcaa gcagttccgt aaagaatgcg cgactggccc
     6721 agccgaccag catcgagtca ttttttcgcg gaaggataat actgacatcc accccgcgct
     6781 gcgccgccgt gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca
     6841 tgatcaaata ttcatgcgcc gaataagccg cagtcaataa tgcctggtga atgagatctt
     6901 ccggaaagcc ggggccagaa gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg
     6961 gcataatatt gacatctggt ggtggcggca gaatacgttt tccggtttca atctcccagt
     7021 cgcaggaata aataatcccc atcgcggtgg cgatggggcc ttccatacgc gccatcagat
     7081 caatccattg ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc atattcatgc
     7141 tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca
     7201 tacggcgtaa aaacacacgc atcagattga cctttaaggc ttcgaccact tcaataccgg
     7261 cattacgcat tagctcgggc cacgggctgc ggaaaaaagc cacactcccg gcggagtcga
     7321 gcatcaatcg gcaatgaata ccacgccgtg cagccgccat taatgattca gccacctggt
     7381 ccgccatgcc gccgggctgc cagatataaa acaccatctc aatattatgg cgcgcgagct
     7441 ggatgtcgcg gattaacgcc tgcatcacat catctgactc ggtcatcagc tgtagctgat
     7501 tccctttgac cccagcgatc ccctgacgac gctcgcaaag cttgaataat ggcgcagcga
     7561 cactgctatt ttcttcggcg aagatatgct tacaggcttt aaggtcgtta agccattttg
     7621 cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc
     7681 caacggcaag ataggcaata attccgacta acggcagaat gtaaataatc aacagccagg
     7741 ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg taaagttacg cctgcaatga
     7801 gcaaccagta tcccagaatg gccaaccaac tcaccaacgt ataaacggtt gtcatagatt
     7861 agaaatcctt ttgaaagcgc attgttatga gtttacgcat atggattcat ctggcaaata
     7921 aaaacgcggg aaaagcactg gtcagcgtcg ggggcggggg tataatgacc attctgttat
     7981 tgcatagagt agttaacatg aagcggagta gaacggaagt ggggcgctgg cgcatgcagc
     8041 gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga aatatgcgta
     8101 tccacagcat caggaagtgc attctaaaca aacagcgtaa ctcgttattg tttgcgatct
     8161 acaatatcta aatgtactgg gcaccattgc ggtgcccaat aagtttactc ttccgtcttt
     8221 aaactctatt tttgcgccga ttctttactg tcagctttcg tttcttgttc atcgttatcg
     8281 ctgaaaatac catgacctga gttcaacaac attgaaacga gcatatcatt attaatctct
     8341 tcaccattta aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga gagaatgata
     8401 tccggatgta ccatcttaat cttattcagg tttttgctat cactgacggc gagaactgtt
     8461 ttgacatcac tgctcatatc ttttgccgag agtacaacaa acgcgttatc tgcatcgtta
     8521 tcactcagcg ccagaatggc ccggcaacga tcgattcccg ctttctttaa tactgaactg
     8581 tcattactgt caccggggat aacatcagcg ttatcgccta agcgttgctc aagttgcttg
     8641 atatcatctt caggcaagtt gctgataacc gttacgtttt gtccgcgttg attcagttgc
     8701 aggatcgtat tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc
     8761 attgtatgat tgtttccttt taaaagtttg ttgaatcccc cgcggataag cggaccaaaa
     8821 attgaggtca tagatgtggc aaaaacggta atgccggaaa taatgaccga aatagtgaac
     8881 aatcgtgctg attcagaaac agggacaata tctccatagc cgacggttga catggtttct
     8941 atcgaaaaat agaacgcggt catcaaactt tctattcgcg gattaaaacc ttcgcttaaa
     9001 taaagcgcac cgtaggttga gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt
     9061 gtcccggctg cggcgctact gtgggagaag tctttgcgca gtatcagcaa aaacaccagc
     9121 gtaaaaatgc aaaatccaat actaaatttc agccagggat aaaaatgcag ggtgtaaatt
     9181 agcgctatca acaacaaaat gatactgatt gcccatgcga gctttgcccg gaacagtaag
     9241 ccaatggagt tcaggacaag aaagacgccg agcataaata gtggggcgtt tgccagcaag
     9301 gagagatcca actctgaaaa tgctttgata tggaaaatat caagcaaatt gaccgacata
     9361 ccatagattg ttttaaaaat aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg
     9421 tgccgtaggg tcacccataa atttgttgct gtttgtttga atgtagccca gtgactcact
     9481 gatacctctg taaccacaat tttgtttaat tatcttagcg tgcacatttt ctgaacatac
     9541 atgcagcgcg tgaatgtgtt aaacaatagt agatgacttt atccgcagta atcaaaggtt
     9601 ttgagtgagc aactctgcgt caggaaaggg cttttttcct gaacatgctc acatcatata
     9661 aagaaataca gataaagtta ttatctgctt gtggtagtga atgcactgac cggctataag
     9721 gaaaggccaa acaagaacac ggttgcaaaa accgtgccct taaatattgg atct
//
LOCUS       DAZSIO010000144         9763 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZSIO010000144 DAZSIO010000000
VERSION     DAZSIO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.144"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            383..1501
                     /locus_tag="ACHX5T_003220"
     CDS             383..1501
                     /locus_tag="ACHX5T_003220"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR8468297.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1567..1815
                     /locus_tag="ACHX5T_003221"
     CDS             1567..1815
                     /locus_tag="ACHX5T_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFR8468298.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1880..2248
                     /locus_tag="ACHX5T_003222"
     CDS             1880..2248
                     /locus_tag="ACHX5T_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR8468299.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFCPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2342..2995
                     /gene="nfsB"
                     /locus_tag="ACHX5T_003223"
     CDS             2342..2995
                     /gene="nfsB"
                     /locus_tag="ACHX5T_003223"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR8468300.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3103..4350
                     /gene="ybdG"
                     /locus_tag="ACHX5T_003224"
     CDS             3103..4350
                     /gene="ybdG"
                     /locus_tag="ACHX5T_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR8468301.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="ACHX5T_003225"
     CDS             complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="ACHX5T_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR8468302.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="ACHX5T_003226"
     CDS             complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="ACHX5T_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR8468303.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKQLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="ACHX5T_003227"
                     /pseudo
     CDS             complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="ACHX5T_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2388 a         2705 c         2368 g         2302 t
ORIGIN      
        1 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
       61 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      121 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      181 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg
      241 ccacattgaa aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa
      301 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      361 aacgtcaact taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac
      421 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      481 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
      541 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
      601 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
      661 aatttgcggc ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa
      721 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
      781 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg
      841 cttgtcacgg tttgtgccgc actttattgc cctttccgcc gcgtcgccat atatgcaggg
      901 aacggatacg cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg
      961 cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtctgagtta
     1021 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
     1081 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat
     1141 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1201 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga
     1261 aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg
     1321 attgctggaa aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc
     1381 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1441 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
     1501 agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac
     1561 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     1621 ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg
     1681 gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg
     1741 tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
     1801 tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc
     1861 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc
     1921 tcttgagtta ccctttgtcg agctttgctg gcctttttgc ccggagttcg atgtttttaa
     1981 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2041 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg
     2101 gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc
     2161 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2221 cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta
     2281 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt
     2341 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
     2401 aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag
     2461 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     2521 tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca
     2581 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     2641 ccaggaagat gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg
     2701 tcgcaagttc ttcgcctata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     2761 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     2821 cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga
     2881 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     2941 cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc
     3001 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
     3061 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3121 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
     3181 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3241 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3301 aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta
     3361 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
     3421 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3481 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     3541 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     3601 attctaatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     3661 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     3721 tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     3781 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     3841 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     3901 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     3961 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4021 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4081 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc
     4141 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     4201 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4261 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4321 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4381 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4441 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4501 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     4561 acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa
     4621 aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg
     4681 ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     4741 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     4801 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     4861 ccccgctgtt atacaccgac agcgatgcca ctagaatgac gaagttcagc gcagaagcta
     4921 ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     4981 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc
     5041 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5101 ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5161 tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5221 ctttctcgcc gccgtaacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5281 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5341 ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5401 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5461 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     5521 aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc
     5581 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     5641 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg
     5701 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     5761 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     5821 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     5881 cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     5941 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggtgcg
     6001 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6061 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac
     6121 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc
     6181 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca
     6241 agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac
     6301 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6361 cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt
     6421 ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6481 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6541 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6601 agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc
     6661 ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc
     6721 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     6781 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     6841 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcagcaagc
     6901 gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg
     6961 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7021 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc
     7081 acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc
     7141 tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7201 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7261 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7321 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7381 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7441 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg
     7501 ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat cactacgatc
     7561 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7621 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7681 ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7741 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagctg tttatgcgca
     7801 ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc
     7861 agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac
     7921 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacggcgcg
     7981 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc
     8041 cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta
     8101 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8161 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc
     8221 cggcgcatct tcgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca
     8281 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgtag atagccgctg
     8341 gcgcgcacca tatattctgc ttccgccagt tcgattgacg aaccgcccgc ttcctggttt
     8401 gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctga
     8461 ggatcgataa ccacctgata ctctttcacc acaccgccta ccgacgccac ttccgcaacg
     8521 tcaggaatgg ttttcagctc atatttgaga aaccagtcct gcaatgagcg taaatcggca
     8581 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
     8641 gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg
     8701 tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac
     8761 acataagagt cgccaaactg cgagaaacca cgcacagtct tcgcgccagg caccgacaac
     8821 atggtggtgg ttagcggata agtcacctga ttttcaacga tttgcggtgc ctggccggga
     8881 tagctggttt taataatcac ctgcacatcg gagagatccg gcaacgcatc cactggcgta
     8941 ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa
     9001 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
     9061 gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
     9121 tcaggaacag gccgccggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
     9181 tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat
     9241 caacggtaat cacccgctgt tcgttgccgg tatcaatcag cgcctgtgac ggaatgagca
     9301 gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct
     9361 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
     9421 gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga acggtgaggg
     9481 taaactgcga ggcatctttc accagccagg cgatggactc cgggatcgca gcagtgaccc
     9541 acaccgggtc cataccctga attcgcgatt acgcgcaatc cccctgagaa ccgccatgaa
     9601 caagggtttc atgatttgtg attttagttt ggtacgcaat ttggtacaca acactatttt
     9661 cacttcagcg ggtagtcatc aaactcacca taacgagcat cgttgatgat gtatgtgatc
     9721 accacaaata tcacatctgg actagttccg tcttcatgcg cag
//
LOCUS       DAZSIO010000145         9682 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZSIO010000145 DAZSIO010000000
VERSION     DAZSIO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.145"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(44..214)
                     /locus_tag="ACHX5T_003228"
                     /pseudo
     CDS             complement(44..214)
                     /locus_tag="ACHX5T_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACHX5T_003229"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACHX5T_003229"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR8468304.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="ACHX5T_003230"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="ACHX5T_003230"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR8468305.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /gene="frdB"
                     /locus_tag="ACHX5T_003231"
     CDS             3536..4270
                     /gene="frdB"
                     /locus_tag="ACHX5T_003231"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR8468306.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="ACHX5T_003232"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="ACHX5T_003232"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR8468307.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="ACHX5T_003233"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="ACHX5T_003233"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR8468308.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5109..6241
                     /locus_tag="ACHX5T_003234"
                     /pseudo
     CDS             5109..6241
                     /locus_tag="ACHX5T_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            6330..6863
                     /gene="blc"
                     /locus_tag="ACHX5T_003235"
     CDS             6330..6863
                     /gene="blc"
                     /locus_tag="ACHX5T_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR8468309.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6860..7177)
                     /gene="sugE"
                     /locus_tag="ACHX5T_003236"
     CDS             complement(6860..7177)
                     /gene="sugE"
                     /locus_tag="ACHX5T_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR8468310.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7353..7499)
                     /gene="ecnB"
                     /locus_tag="ACHX5T_003237"
     CDS             complement(7353..7499)
                     /gene="ecnB"
                     /locus_tag="ACHX5T_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR8468311.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7610..7735)
                     /gene="ecnA"
                     /locus_tag="ACHX5T_003238"
     CDS             complement(7610..7735)
                     /gene="ecnA"
                     /locus_tag="ACHX5T_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR8468312.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7787..8353)
                     /gene="efp"
                     /locus_tag="ACHX5T_003239"
     CDS             complement(7787..8353)
                     /gene="efp"
                     /locus_tag="ACHX5T_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR8468313.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8395..9423
                     /gene="epmB"
                     /locus_tag="ACHX5T_003240"
     CDS             8395..9423
                     /gene="epmB"
                     /locus_tag="ACHX5T_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR8468314.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2465 a         2480 c         2466 g         2271 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac
     5101 cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat
     5161 ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
     5221 aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta
     5281 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
     5341 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
     5401 aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga
     5461 atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc
     5521 cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat
     5581 gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg
     5641 ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac
     5701 tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg
     5761 gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg
     5821 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta ccccccttga
     5881 tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct actggcaaac
     5941 cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca atcctgacag
     6001 catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa aagcgattaa
     6061 gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga ccggcggatt
     6121 tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc tggctaacaa
     6181 aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg ctctacagta
     6241 aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct gtcatctaca
     6301 cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt gccgcagcga
     6361 cagctgcatt tctggtcgtt gcctgtagtt ttcctacgcc gccgcgtggc gtgaccgtag
     6421 tgaataattt cgacgccaaa cgttatctcg gtacctggta tgagattgcc cgttttgatc
     6481 accgttttga acgtggactg gaaaaagtca ccgcaacata cagcctgcgt gatgacggcg
     6541 gcctgaatgt cattaataaa ggctataacc ctgacagagg aatgtggcag cagagtgaag
     6601 ggaaagcgta ctttaccggc gcaccaactc gcgctgcgct gaaagtgtca ttctttggtc
     6661 ctttctatgg cggttataac gttattgcac tcgatcggga ataccgccat gcgctggttt
     6721 gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt tctgacgaag
     6781 tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt aagtttattt
     6841 gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc cccaatacga
     6901 ttaacgccag actcgccaga cgcatcgggt tagcggactc accgagcagc acaatgccgg
     6961 tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc cctaccggta
     7021 acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc acggtaataa
     7081 cactcggcgt caaacggcta aagccgtggg tatatttcag gccaacggcc cataccactt
     7141 ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga aatttggggc
     7201 cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt tgaacggata
     7261 ttttgccagg tggcagggga atttcaacta ttacctttat gacgatcgca gaaaaaacaa
     7321 acggcacgac actgctgtcg taccgttatt gcttattgct gcgctttcgt tgctgcgcca
     7381 gaaatcgcgt taccgccatc agaaatgtct tcaccaacgc cacgcgtggt gttgcaggca
     7441 gttaatactg ttgaaagcac cagaacagaa aagatcgctg caattgtctt cttcaccata
     7501 acgtcttcct tttaggtaat gttatttatg tttgcctata gcaaattaat aatagacaac
     7561 atcgccaaaa atgatggaaa tctgatgatt tttcggaaga gaagaaaaat tagctggcag
     7621 cgcgagagat ggagttaccg agatgtttga tgtcttcgcc gaaaccgcaa gcggtgttac
     7681 agcccgtgag cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc atcatctctg
     7741 acctgaataa gtgatggtgc agcctgcagg ccgcaccaca accgcattac ttcacgcgag
     7801 agacgtattc accagagcgg gtatccactt tgatgacttc gccgatttgt acgaacagcg
     7861 gaactttaac cacagcgcca gtagacaggg tagccggttt gccgccagta cctgcggtat
     7921 cacctttcag gcccggatcg gtatcaacga tttccagttc aacgaagttc ggcggagtaa
     7981 cggagatcgg ctgaccattc cacagagtta cgatacactc tgcctgatcc agcagccatt
     8041 tagcattgtc gccaattgct tttgcatcag cagacagctg ctcgaaagtt tcgttgttca
     8101 tgaagtgcca gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg acaacatcag
     8161 cgccttcagc ggaatcagta gatttgaagg ttttttctac gcgagtaccg gtcagcagac
     8221 gacgcagttt aacgcgagca aatgcctggc ctttacccgg ttttacgaat tcactcgctt
     8281 caaccgcgta aggttcgccg tctaacatga ttttaagacc agcacgaaaa tcgttgctat
     8341 agtacgttgc cataaggccc tctgaaattt gttaattggt agctaagcca caaaatggcg
     8401 catattgtaa ccctaaatac cccatccaga gaagattggt taacgcaact tgccgatgtt
     8461 gtgaccgatc ctgatgaact tctgcgtctt ttgaatatag acgcggacga aaaactgttg
     8521 gccggacgca gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt tatcgatcgc
     8581 atggagaaag gcaatccgga cgatcctctt ttgcgtcagg tacttacctc gcaagatgag
     8641 tttgtcgtcg cgtccggatt ctccaccgac ccactggaag aacagcacag cgtagtgcct
     8701 ggtttgttgc ataaatacca caaccgggcg cttttgctgg tcaaaggcgg ctgcgcggta
     8761 aattgccgct attgcttccg tcgccacttc ccctatgccg aaaatcaggg caacaagcgt
     8821 aactggcaga ctgcacttga gtatgttgct gcgcatccgg aactggacga gatgattttc
     8881 tccggcggcg atccgctgat ggcgaaagag cacgagctgg actggttgct cacacaactg
     8941 gaagccatcc cgcatatcaa acgtctgcgg attcacagcc gtctgccgat tgtgatcccg
     9001 gcacgtatca ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca aatcttgctg
     9061 gtgaatcaca tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc gatggctaaa
     9121 ttgcgccgtg tcggtgtcac cctgcttaac cagagcgttc tgttacgtgg tgtgaacgat
     9181 aacgcacaaa cgttggcaaa cctgagtaat gcgttgttcg atgccggcgt gatgccgtat
     9241 tacctgcatg tgctagataa agtacagggc gcggcgcatt ttatggtgag tgatgacgaa
     9301 gcacggcaga ttatgcgtga gttgctgaca ttggtgtcgg gatatctggt gccgaaactg
     9361 gcgcgagaaa ttggcggcga acccagcaaa acgccgctgg atctccagct acgccagcag
     9421 taactgatta aacagggaag ttaaacatta aaattgcctg atgcgctacg cttatcaggc
     9481 ctacatcgtg cattgaaatt gaatggattt gcaatgaact gtaggccgga taaggcgttt
     9541 acgccgcatc cggcaagagc aacaaacgca agtacagcaa ccactgagaa atttaatctt
     9601 tgcacgttgc ataaccttta attattcata tgcattgctg ttgttacttt ttatatagct
     9661 aacaccaata attaaccttt ct
//
LOCUS       DAZSIO010000146         9572 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZSIO010000146 DAZSIO010000000
VERSION     DAZSIO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.146"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..133
                     /locus_tag="ACHX5T_003241"
     CDS             <1..133
                     /locus_tag="ACHX5T_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR8468315.1"
                     /translation="SNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV"
     gene            230..382
                     /gene="hokE"
                     /locus_tag="ACHX5T_003242"
     CDS             230..382
                     /gene="hokE"
                     /locus_tag="ACHX5T_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR8468316.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            733..885
                     /gene="hokE"
                     /locus_tag="ACHX5T_003243"
     CDS             733..885
                     /gene="hokE"
                     /locus_tag="ACHX5T_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR8468317.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(1007..1627)
                     /gene="entD"
                     /locus_tag="ACHX5T_003244"
     CDS             complement(1007..1627)
                     /gene="entD"
                     /locus_tag="ACHX5T_003244"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR8468318.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1802..4042)
                     /gene="fepA"
                     /locus_tag="ACHX5T_003245"
     CDS             complement(1802..4042)
                     /gene="fepA"
                     /locus_tag="ACHX5T_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR8468319.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4285..5487
                     /gene="fes"
                     /locus_tag="ACHX5T_003246"
     CDS             4285..5487
                     /gene="fes"
                     /locus_tag="ACHX5T_003246"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR8468320.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5490..5708
                     /gene="ybdZ"
                     /locus_tag="ACHX5T_003247"
     CDS             5490..5708
                     /gene="ybdZ"
                     /locus_tag="ACHX5T_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR8468321.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5705..9550
                     /gene="entF"
                     /locus_tag="ACHX5T_003248"
     CDS             5705..9550
                     /gene="entF"
                     /locus_tag="ACHX5T_003248"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR8468322.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         2105 a         2505 c         2630 g         2332 t
ORIGIN      
        1 cagtaatata agcgaactga aggatgctgt tacggaatat attgaatact ataacagcag
       61 aagaattagc ctgaaattaa aaggtctgac tccaattgaa tatcggaatc agacctatat
      121 gcctcgtgta taactgtcca actttttggg gtcagtacac cctggggctg ccactccaaa
      181 ccagacaatt tggatggtag tcccttcttc gcatggaggt aatataaaca tgctgacgaa
      241 atatgccctt gtggcagtca tagtgctgtg tttgacggta ctgggattta cgcttctggc
      301 cggagactcg ctgtgtgagt ttacggtgaa ggaacgtaat attgagttca gggctgttct
      361 cgcttacgaa ccgaagaagt agccgttgtg cggggagtaa tccccgcata tccggttgtc
      421 aggtcaggat ggtaaggcac ctgctttaca ctttcgcccg cggtcagtga tggctgcggg
      481 cgatttcgtt ttaagagcaa tgatgagttt tgccgacaga agtccgaaac aggactcact
      541 gtataaataa acagctattt tgctaaggaa gggtaagata acggcgggtg cctgaagctt
      601 tccggtttca ggttaactct gaggtttgga aagatgaagc cccaagagat atttctatca
      661 atcctggggc tgccactccg aacctcaaca actcggatgg tagtcccttc ttcgcatgga
      721 ggcaatataa acatgctgac gaaatatgcc cttgtggcag tcatagtgct gtgtttgacg
      781 gtgctgggat ttacgcttct ggtcggagac tcgctgtgtg agttcaccgt aaaggaacgt
      841 aatattgagt ttaaggctgt tcttgcttac gaaccgaaga agtagccgtt gtgcggggag
      901 taatccccgc atatccggtt gtcaggtcag agtggtaagg cacctgcttt acactttcgc
      961 ccgcagtcag tgatggctgc gggcgaatcg taccagatgt tgtcaattaa tcgtgttggc
     1021 acagagttat gactattttt tcttttatct gccagtgcac agcaaacatc tcattctcac
     1081 gatgaatgat gacctgctgt ttattccagc taattatctg atagtccaga aaacctgcat
     1141 cagtttggat ctcacttgcc ttaaatgcgc tctctttggc ggaaaatgca agtgtcagcg
     1201 ccagagaaaa ggctaaaccg cagtctgcga gtcgctcgtg ttctgctggt gtaataatgt
     1261 tgtctgtcaa ttcggtcgct gtttgcgcag aaaaaatttc ttcgatatca acgccaatcg
     1321 gttgacggga aaccacggcc aatgccgtag ccccgcagtg gctaatactg ccgtatacct
     1381 ccgcaggcca caccggttgg cgtagctcgc cgattgcggg cacacattta tagccatatt
     1441 cccgcaaggc ataaacagca gcgatccgtc cggctaaatg ctctgtttta cgtttacgtc
     1501 cagcgtgttg cagttgtgcg tagtgcggca gccagagtaa atcctgctca caaaaattcg
     1561 ccggatcgaa ctcaacaaaa tgcagcgtat gtccggcaaa ggggagggag gtatgcgtag
     1621 ttttcatatc gaccatattc gagactgatg acaaacgcaa aactgcctga tgcgctacgc
     1681 ttatcaggcc tacgtgatcc tgcaatatat tgaattggca agatctttgt aggccggata
     1741 aggcgttcac gccgcatccg gcgtgaacga cgcgcacttt gtcaacaatc tgacgttagc
     1801 atcagaagtg agtattaatg ctcatatacc acgtacgtcc cggctcgtta taggtatacg
     1861 cgccggcacc ggcgatatag ttggctcctg ccagatcgcc cgtggtctgg gcattacctg
     1921 cacgccacaa acgtttgtcg aacaggttgt ccacgccgcc ggtcagactg acattcttcg
     1981 tcacatccca ggtcgcgctc agacccacaa tgctgtaagg actgatttct ttggtttccg
     2041 ggccaaccgc tggctgacct ttatagttgt acttcttcgg ctgctgcttg ccgtaccagg
     2101 tgaaggtcgt ttgcatcgac aaatcttccc gtgcctgcca gctcagcgtt gagttcaacg
     2161 tgtactccgg gatgatcgac aaacggtcgc ccgtggtttt gttttcactc ttcagcatat
     2221 aagtgatgtt attggtccac atcaccgttt cgctaactgg tacgtttaac gatccttcca
     2281 gaccttcaac cactgctttc ggcacgttat cccactgata gagatcagtg ccgactgcgt
     2341 tttgccctac agccacatag cccgcttcaa tcttattgcg ataatcgtta cggaaccagg
     2401 tgacgcccgc cagccagccg tcgcgtttga actccagacc aatctctttg ttgatgctgg
     2461 tttctacttt caggtcatcg ttaccttgca gatagcagcc gcccgcgctg gcatagcagc
     2521 cctgaccttt actgtagaga atgtagttcg ggttagtctg gtataggctc ggcgctttat
     2581 aggcgcgggc aatgcctatt ttcagcgtga agtcatcgcc taaaccttgc gatatgttca
     2641 gcgccgggct ccagttattg ccgacaatac tgtgatgatc gaaacgcagc cccggcgtta
     2701 cgatggtgct gtcagtcagc tccatgttgt tttcggcaaa cagcgagaaa atttctgctt
     2761 ttgaatacgg gctacggtcg gtggcactca cgccatcaat agcgccaccg gtattcgttc
     2821 cggtcagtgc ttgggtgttg gaacttaagt ccttcatccg ttgctgattc cactccgtac
     2881 ccagcgtcag cgtctggtta acgaggaaat caatcggcag gttaacttcg ctgtgcagca
     2941 tcacgtcatc gagatcgata tcgacgaaat cctgtgtcgc tttttcgtta aatttccctt
     3001 cggtaccgcc cgccagacct tccggaatac gcgagttacg ggtgtgttca tactgcaccc
     3061 agttgctggt ggtcacgccg ttatcccagc caccgttcca ggtcagcgcg tagttctggc
     3121 gatacaggcg gttggtttca tcgccatatt tcgagcgagt gtaagcgtca gagttggtgt
     3181 tctgggtatc gcccgcatac aggttaccct ggcggctgta acccgcttcc agttccagcg
     3241 attgcaatgg tgcgaagtcc cagcgcacca cgccgttaat atctttattg atcacccctt
     3301 cgcgcccggc tggtaacgtc gtggcatacg ttccggcacg cgcggactga tggccctggt
     3361 tgatatccca cgcgtcagcc tgagttttgt cgaggttgcc atacaagcgg aagctgaatt
     3421 cgtcgcccag cggaccggtc aggctaaagt tagtgcgttt ggtggcacct tcctctttat
     3481 gttctggcgc attgaaatat gcgtcccagg agccgtgcca ctcgccgctg ccttttttgg
     3541 taatgatgtt aaccacgccg cccgccgcgc cgttgccata acgcgcagct gccggaccac
     3601 gcagaacttc aatacgttca atcatttcag gtggcaccca ggaggtatca ccacgggtat
     3661 cgcgctcgcc acgccagccc tgacgcaccg agttacggct gcttaccggc ttgccgtcaa
     3721 tcaaaatcag cgtgttttcc ggacccatac cgcgaatatc aatctgtcgg ttattcccac
     3781 gctgaccact ggtggagtta ccggtcaggt taacgcctgg catggtacgg atgatctccg
     3841 acacatcgcg ggcaaccggg tttttgcgga tttcatctgc ggtgatggtc gaaacgccag
     3901 gagcctgcaa gttctgctcg gcggcggtaa cgacaatggt atcgtcatgt gaaacaggtg
     3961 tatcggtcgg ctcttgtgcc tgcgctaccc cataaatccc cagattgacc aacaaggcca
     4021 gggaatgaat cttcttgttc attgttttat tcctgcattt ttgccacgaa ttgcaactgt
     4081 cgggcatggt cgtcatcaac acgacgcatc ccgttaccgc gaaaaccttt gatcctgaaa
     4141 gacacgcagt gcagttggtt aattaatgtc cgcgcttccc acggcgcgcc attacgctat
     4201 tgcaaatgca aatagttatt aataacatta tcaatatatt tctgcaatca atgaaaaatt
     4261 gcacagtaaa catggggtta tggtgtgacg gcgttaaaag taggaagtga gagctggtgg
     4321 cagtcgaaac atggcccgga atggcagcgt ctgaatgacg aaatgtttga ggtcactttc
     4381 tggtggcgtg atccccaagg ttctgaagaa tactcgacga taaagcgcgt atgggtctac
     4441 atcactggtg tgaccgatca tcatcagaac agccagcccc agtcgatgca gcgaattgca
     4501 ggtactgatg tctggcagtg gacgacacaa ctcaatgcca actggcgcgg cagctactgc
     4561 tttattccca ccgaacgcga tgacattttt tctgcaccat cccccgatcg cctcgaatta
     4621 cgtgaaggct ggcgaaaact attaccccag gcaatagccg atccgctgaa tccacaaagc
     4681 tggaaaggcg ggctagggca cgctgtttct gcactcgaaa tgccgcaagc gcctctgcaa
     4741 ccgggatggg attgtccgca agcgccagaa ataccagcca aagaaattat ctggaaaagt
     4801 gaacggttga aaaattcacg tcgtgtatgg atttttacca ccggcgatgt aacagcagaa
     4861 gaacgcccgc tggcagtttt gctcgatggc gaattttggg cgcaaagtat gcccgtctgg
     4921 cctgtgctga cttcgctgac ccatcgtcag caacttcctc ccgccgtgta tgtgttgatc
     4981 gacgctatcg acaccacgca ccgcgcccac gaactgccgt gtaatgcgga tttctggctg
     5041 gcagtacagc aagagttatt acccctggtg aaagttattg ccccttttag cgatcgcgcc
     5101 gatcgcaccg tggtcgccgg gcagagtttt ggtgggctgt ccgcgctgta tgccggactg
     5161 cactggcctg aacgctttgg ctgtgtatta agccagtcag gatcgtactg gtggccgcat
     5221 cggggcgggc agcaagaggg cgtgttactt gaaaagctaa aagctggtga agttagcgcc
     5281 gaaggtctgc gcattgtgct ggaagcgggt attcgcgagc cgatgatcat gcgggccaat
     5341 caggcgctgt atgcgcaatt acaccctata aaagaatcca ttttctggcg tcaggttgac
     5401 ggcggacatg atgcgctttg ttggcgcggt ggcttgatgc aggggttaat cgacctctgg
     5461 caaccacttt tccatgacag gagttgaata tggcattcag taatcccttc gatgatccac
     5521 agggagcgtt ttacattttg cgcaatgcgc aggggcaatt cagtctgtgg ccgcaacaat
     5581 gcgccttacc ggcaggctgg gacattgtgt gtcagccgca gtcacaagag tcctgccagc
     5641 agtggcttga agcccactgg cgtactctga caccggcgaa tttcacccag ttgcaggagg
     5701 cacaatgagc cagcatttac ctttggtcgc cgcacagccc ggcatctgga tggcagaaaa
     5761 actgtcggat ttaccctccg cctggagcgt ggcgcattac gttgagttaa ccggagaggt
     5821 tgatgcgcca ttactggccc gcgcggtggt tgccggatta gcgcaagcag atactttgcg
     5881 gatgcgtttt acggaagata acggcgaagt ctggcaatgg gtcgatgatg cgctgatatt
     5941 cgaactgcca gaaattatcg acctgcgaac caatattgat ccgcacggta ctgcgcaggc
     6001 attaatgctg gcggatttgc aacaagattt gcgcgtcgat agcggtaaac cactggtctt
     6061 tcatcaactg atacaggtgg cagataaccg ctggtactgg tatcagcgtt atcaccattt
     6121 actggtcgat ggcttcagtt ttccggccat tacccgccag atcgccaaca tttactgcgc
     6181 attgctgcgt ggcgaaccaa cgcctgcttc gccgtttacg cctttcgctg atgtagtgga
     6241 agagtaccag caataccgcg aaagcgaagc ctggcagcgt gatgcggcat tctgggtgga
     6301 acagcgtcgc caactgccac cgcccgcgtc actttctccg gcacctttag cggggcgcag
     6361 cgcctcggca gatattttgc gtctgaaaat ggaatttacc gacggggaat tccgccagct
     6421 ggctacgcaa ctttcaggtg tgcagcgtac cgatttagcc cttgcgctga cggctttgtg
     6481 gctggggcga ttgtgcaatc gcatggacta cgccgccgga tttatcttta tgcgtcgact
     6541 gggctcggcg gcgctgacgg ctaccggacc cgtgctcaac gttttgccgc tgggtattca
     6601 tattgcggcg caagaaacgc tgccggaact ggcaacccga ctggcaaccc gactggcagc
     6661 acaactgaaa aaaatgcgtc gtcatcaacg ttacgatgcc gaacaaattg tccgtgacag
     6721 cgggcgagcg gcaggtgatg aaccgctgtt tggtccggta ctcaatatta aggtatttga
     6781 ttaccaactg gatattcctg gtgttcaggc gcaaacccat accctggcaa ccggtccggt
     6841 taatgacctt gaactggccc tgttcccgga tgaacacggt gatttgagta ttgagatcct
     6901 cgccaataaa cagcgttacg atgagccaac gttaatccag catgctgaac gcctgaaaat
     6961 gctgattgcc cagttcgccg cggatccggc gctgttgtgc ggcgatgtcg atattatgct
     7021 gccaggtgag tatgcgcagc tggcgcagat caacgccact caggttgaga ttccagaaac
     7081 cacgctgagc gcgctggtgg cagaacaagc ggcaaaaaca ccggatgctc cggcgctggc
     7141 agatgcgcgt tacctgttca gctatcggga aatgcacgag caggtggtgg cgctggcgaa
     7201 tctgctgcgt gagcgcggcg ttaaaccagg ggacagcgtg gcggtggcac taccgcgctc
     7261 ggtctttttg accctggcac tccatgcaat agttgaagct ggcgcggcct ggctaccgct
     7321 ggataccggc tatccggacg atcgcctgaa aatgatgctg gaagatgcgc gtccgtcgct
     7381 gttaattacc accgacgatc aactgccgcg ctttagcgat gttcccaatt taaccaacct
     7441 ttgctataac gccccgctta ccccccaggg cagtgcgccg ctgcaacttt cacaaccgca
     7501 ccacacggct tatatcatct ttacctctgg ctccaccggc aggccgaaag gggtaatggt
     7561 cgggcagacg gctatcgtca accgcctgtt gtggattcaa aatcattatc cgctcacagg
     7621 cgaagatgtc gttgcccaaa aaacgccgtg cagttttgat gtctcggtgt gggagttttt
     7681 ctggccgttt attgccgggg ctaaactggt gatggctgaa ccggaagcgc accgcgaccc
     7741 gctcgctatg cagcaattct ttgccgaata tggcgtaacg accacgcact ttgtgccgtc
     7801 gatgctggca gcatttgttg cctcgctgac tccgcaaacc gctcgccaga gttgcgtgac
     7861 gttgaaacag gttttctgta gtggtgaggc cttaccggct gatttatgcc gcgaatggca
     7921 acagttaacc ggcgcgccgt tgcataacct gtatggcccg acggaagcgg cggtagatgt
     7981 cagctggtat ccggcttttg gcgaggaact ggcacaggtg cgcggcagca gtgtgccgat
     8041 tggttatccg gtatggaata cgggtctgcg tattcttgat gcgatgatgc atccggtgcc
     8101 gccgggtgtg gcgggtgatc tctatctcac cggtattcaa ctggcgcagg ggtatcttgg
     8161 acgacccgat ctgaccgcca gccgctttat tgccgatcct tttgctcctg gtgaacggat
     8221 gtatcgtacc ggagacgttg cccgctggct ggataacggc gcggtggagt acctcgggcg
     8281 cagtgacgat cagctaaaaa ttcgcgggca gcgtatcgaa ctgggcgaaa tcgatcgcgt
     8341 gatgcaggcg ctgccggatg tcaaacaagc cgttacccac gcctgtgtga ttaaccaggc
     8401 ggcagccact ggtggtgatg cgcgtcagtt ggtgggctat ctggtatcgc aatcgggcct
     8461 gccgttggat accagcgcat tgcaggcgca gcttcgcgaa acattgccgc cgcatatggt
     8521 gccagtcgtt ctgctgcaac tgccgcagtt gccacttagc gccaacggca agctggatcg
     8581 caaagcctta ccgttgcctg aactgaaggc acaagcgcca gggcgtgcgc cgaaagcggg
     8641 cagtgaaacg attatcgccg cggcattcgc gtcgttgctg ggttgtgacg tgcaggatgc
     8701 cgatgctgat ttctttgcgc tgggcggtca ttcgctactg gcaatgaaac tggcagcgca
     8761 gttaagtcga cagtttgctc gtcaggtgac gccggggcag gtgatggtcg cgtcaaccgt
     8821 cgccaaactg gcaacgatta ttgatggtga agaggacagc tcccggcgca tgggattcga
     8881 aaccattctg ccgttgcgtg aaggtaatgg cccgacgctg ttttgtttcc atccggcgtc
     8941 cggttttgcc tggcagttta gcgtgctctc gcgttatctc gatccacaat ggtcgattat
     9001 tggcattcag tcgccgcgcc ctcatggacc catgcagaca gcgacaaacc tggatgaagt
     9061 ctgcgaagcg catctggcaa cgctacttga gcaacaaccg cacggcattg cggcgcggct
     9121 gcgtgcccgt ggcgaacagg tggcatttct tggtttgctg gatacctggc cgccagagac
     9181 gcaaaactgg caggaaaaag aggctaatgg tctggacccg gaagtgctgg cggagattaa
     9241 ccgcgaacgc gaggccttcc tggcggcaca gcagggaagt acttcaacgg agttgtttac
     9301 caccattgaa ggcaactacg ctgatgctgt gcgcctgctg acgactgctc atagcgtacc
     9361 gtttgatggt aaagcgacgc tgtttgttgc tgaacgcacg cttcaggaag gtatgagccc
     9421 cgaacgcgcc tggtcgccgt ggattgcgga gctggatatc tatcgtcagg attgtgcgca
     9481 tgtggatatt atctctccag gggcgtttgt aaaaattggg ccgattattc gcgcaacgct
     9541 aaacaggtaa attaatatta tctataaacc ca
//
LOCUS       DAZSIO010000147         9537 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZSIO010000147 DAZSIO010000000
VERSION     DAZSIO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.147"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(54..533)
                     /gene="xdhC"
                     /locus_tag="ACHX5T_003249"
     CDS             complement(54..533)
                     /gene="xdhC"
                     /locus_tag="ACHX5T_003249"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR8468323.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="ACHX5T_003250"
     CDS             complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="ACHX5T_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR8468324.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="ACHX5T_003251"
                     /pseudo
     CDS             complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="ACHX5T_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            4131..4886
                     /gene="actS"
                     /locus_tag="ACHX5T_003252"
     CDS             4131..4886
                     /gene="actS"
                     /locus_tag="ACHX5T_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR8468325.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4965..5038
                     /locus_tag="ACHX5T_003253"
     tRNA            4965..5038
                     /locus_tag="ACHX5T_003253"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4997..4999,aa:Gly,seq:ccc)
     gene            5173..6783
                     /locus_tag="ACHX5T_003254"
     CDS             5173..6783
                     /locus_tag="ACHX5T_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8468326.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6815..7234
                     /locus_tag="ACHX5T_003255"
     CDS             6815..7234
                     /locus_tag="ACHX5T_003255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468327.1"
                     /translation="MVKEFLVSKENKPSYECMNTAETCLGLAGMASEDWQREHHCRTG
                     IIFTAFAIEAMFIFYRKQVDPGYDKTQKECRKTMHKNTLKLCGINNYMGTKPYQIIKE
                     CLEVRDAIAHGDSYTSSFNFSADHLDNQDDIAKVSWR"
     gene            8353..9099
                     /locus_tag="ACHX5T_003256"
     CDS             8353..9099
                     /locus_tag="ACHX5T_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR8468328.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
     gene            9397..>9537
                     /locus_tag="ACHX5T_003257"
     CDS             9397..>9537
                     /locus_tag="ACHX5T_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468329.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLP"
BASE COUNT         2432 a         2362 c         2422 g         2321 t
ORIGIN      
        1 tgttaaacac ataacctcag tgaatccggc ctgaattcag gccggatatc cttttacttc
       61 gttttctcgc aatccagaac tgtatttaca atcatctgat accccgtgca gcgacaaaga
      121 tttcccgcca gtccgcgacg aatttccgta atggttaatg gcttctcgcg tggtttcgcc
      181 agcattgccg tggtagccat aatcaggcca ggcgtacaaa acccgcactg cactgtgccg
      241 gatttcgcat aagcctgctg aacatgagaa agttttccgc ctttcgcttc accttccagc
      301 gtgcggatct cttttccttc agcccaggcg gcaaggtata agcaactgtc tattgctgtg
      361 ccgtcgacca acaccgtaca ggcaccacat tcacccacgc agcacccttg tttgacactt
      421 agcagtcctt gttcgcggag taattccgag agcggcgtgc ctggtgcggc gtgaagctga
      481 aaaggcatcc cgttaatggt gcattcggtg gtaattgttt cgctgtgatt cattgcaatt
      541 ttccccccgc cgcggcgacg gcttcgctaa tcactttttt ggtcatcgtc tggatgagat
      601 gcagacgaaa ctctttactg gcccgccatg aagaacgcgg ggcgacatct tgcaggacag
      661 attcgctgat agcttccagc gtttgcaggt ttaatggcgc attttgtgca gtctgttcgg
      721 catgttggca gcgaatcggc gttggcgcgg caacaccaaa tgccaggcgt aattcgctga
      781 aattgccgtt atccagtcgg caatgtgcgg cgcagccaat cgttgaaata tccattgcgt
      841 cgcgcatggc atatttaaaa tgcgcgctgc ccgcgtgttc tttcggctgt ggcggaaaat
      901 gaaaggcgac gaggatttcg tcatgctcaa gagacacttt gcccggcccg gtgtgaaagc
      961 cattaatcgg gacgaaacga acaccgcgcg gggagtggat ctccagtttc gcgtcataaa
     1021 ttagcgttgg cgtggcagaa tctgcgctgg tggcaccgtt gcaaatattt ccaccgtagg
     1081 tagcgacgtt acggatctgc ggtccagcaa tgaacgtggc cgcagcacat aacgccggga
     1141 gatgacgttg agttatagga tcttctatta gctgggtaaa tgtcgttgca gagccgatac
     1201 gtagcgagcc atcttccgcc agcgtaattc cccgcagctc cgccagatta tgaatatcaa
     1261 caatatggcg ataacggtca ttgtgatggt ggagctgaat cagtacgtca gtgccaccgg
     1321 cgagcagttt ggcctgcggg ttgtcagcca gcaggttgat ggcatcggca agggttgctg
     1381 cgcgatggta agaagcaaaa tcaaacatga tgttatcctc aaatcaatcc tgccagatgg
     1441 aactcttcat ataaccgttt tggcgtcagc ggcagtgtat tgattgcaac accggtagcc
     1501 atcttcaccg cgttacgaat agcagcggca acaggaatta tgggtggctc acccagtgac
     1561 ttatgtccgt atgcggattg cggctcattg atttcgacga acgcgctttc cagttgtggc
     1621 agatccggca tggtcggcat tttgtaatcc agcagattgg ggttacggac cacgccgctt
     1681 ttagcatcga tgatcatctc ttcaaatagc gcccagccaa tgcccattcc cattccgccg
     1741 tgtacctgac cttctgccag cagtggatta agaatatgcc ctgaatcatg aacgttgagg
     1801 atgcggttga tggtgacttt gcacagcgca atatcgaccg tcagatcaac aaaggtacag
     1861 ccaaacgccg gtgggttagt ggtggttttg atggagcttt cagcagagag ctgcccgccg
     1921 cgttcagggt ggtagaaagc gtccatcgcc aaatctttta acgacattaa cggctcttcc
     1981 ggtcgttcaa ccagcacgat atggcctttt atcagggtca aattcatcgc tgactgatgt
     2041 agcatgactg cggcgtgagc gatgattttc tcttttaata atagtgccgc actgcgcagc
     2101 gcaggcgcgg caacatagct ctggcgtgag gcaaatgcgc cgggatcgaa cggcgtaacg
     2161 tcggtatctt gtgttgaaat aacgcgaacg tcgctgaccg gaacccccac ggtttctgcc
     2221 accatttgcg agaagacggt gtcggcaccc tgaccgattt ccgtcgcgcc gctttgcacg
     2281 ttgatggttc catcctgatt catcagaagg cgcgcgcctg ctatttctac gccgacaggc
     2341 caggtgttag aggtgtagct aaaacaggcg acgccaacgc cgcggcgcaa attgccttgc
     2401 tggttctggc attctgcacg gcgtttttcc cattcaaaga ttttccggcc tttttcaaga
     2461 cactccggca accctgcgct gcaaatacgt ttgcccgtga gcggattagc atctccttcg
     2521 cgggcggcgt tgcgtaaacg aatttcaaca ggatcaatac ctaacgctgt cgcggcgtca
     2581 tcaagcatag actcaacggc aaatacgact tgtggcgcgc cataaccacg catcgcacca
     2641 gccgagggga ggttggtata gcaggtcttt gaactgtaag cgtaggcaca acgaggataa
     2701 aggtaagcga ctttattccc ccccgcagaa gcgatggagt gcccgtgaga tgcataagcg
     2761 ccggtgttag acagaacatc cagactataa cctttcaagg ttccgtcgcg gttcacgccc
     2821 atttgcccgt caatagtaaa agcgtggcgg gtacgggttg cgaggaaaca ctcttcacgg
     2881 ctaagggaaa ctttcaccgg aatgccgcca agcttgctgg tcaggaatgc cgccattggc
     2941 tcttccagta catcctgttt attaccaaaa ccgccaccga caaacggttt gatgactcgt
     3001 acgcatgacc agggaatatc cagcgcctga ccaaccacgc ggcgaacaat gtgcgggatc
     3061 tgggtgctgg aaacgatggt aattcgcgag tcatcctcca tccacgccag cgatgttacg
     3121 ctttccatgt gacaatgttg aataacggga gtctgatagt gcccctgtac ctggtagtcg
     3181 gcggcatcga ttgtttgttg gacattgccc gtcgacatcg tgctttgttt cagtaaattg
     3241 ccgccgttat ggattggtgc agcgtcttct gtcagcgccg cttctggcgt ggtgataacg
     3301 ggtaattctt gccactcaat gctgaccaat tgcgccgctt tttctgccgt gagttcatcg
     3361 cgggccacga cgatggcaac ggcgtcacca tgatgacgaa catggcgagt tagcagtgcg
     3421 cgatcggcgg tatcgcgctt gttttcgtca agtgtctagg catgcccagc tgtagcgaat
     3481 ggaatatcag gcacatcttc ccaagtaaaa atcgccagta cgcccggtaa acttctggct
     3541 tgttcatcat taatacttac ggcataacca tgtgcgatag ggctacgtac atatttcgcg
     3601 taacacatgc ccgccataac ataatcgtca gtatatcgtg cccgcccggt gaccttagca
     3661 atggcatcga cgcgcatgca tgattcaccc gtagcggttg cttcccgcgc ttccatagaa
     3721 atcccctcga tttgaacatt tggttcttat ggcaaagatt tagtctaagc aggcaagatt
     3781 tacgccagaa acgacgataa gcgttagggg aggaaggaag tgtgatgacg ttaaaaaaac
     3841 acagatatga tacgaaacag gactctgaat agggacagtg atacgtatca atatgagtgg
     3901 cgataaaatc aatgtgataa atcaggatta ttacgcatca ggatgggaaa tagggtaaaa
     3961 agtcaggaac ttcgatacct ctacagcgaa tattttgctg atgatacttc cctttgccaa
     4021 atgaaattaa cctctttctc tattaccacg tttttccaga agcaagagat tgggtatcaa
     4081 gaggctggct gctatgatat ggcgtcttgt tttttaagcg aggaaagatt ttgagtgcgg
     4141 gacgcctgaa taaaaaatct ctgggtatcg tgatgttttt atcggttgga ctgctattgg
     4201 cgggctgttc gggtagcaaa tcatccgata caggaacgta ttccggctcc gtttacaccg
     4261 tgaaacgggg ggatacgcta tatcgtattt cgcgcaccac gggaaccagc gtaaaagagc
     4321 tggcgcgact gaacggcatt tccccccctt acaccattga agttggtcag aaactaaaac
     4381 tgggtggggc gaaaagtagc agtagtacac gtaaatcaac cgccaaatca acgactaaaa
     4441 ccgcatcggt tacaccgtca tcagcggtac cgaaatcttc ctggccgcca gtagggcaac
     4501 gttgttggtt atggccaacg acagggaaag ttatcatgcc gtattcgaca gcagatggcg
     4561 gcaataaagg gattgatatc tcagctccac ggggtacacc tatttacgcc gcgggtgcag
     4621 gaaaggtggt gtatgtgggc aaccagctgc gtggctacgg taatctcatc atgattaaac
     4681 acagtgaaga ttacattacg gcttacgccc ataatgacac gatgctggta aataatgggc
     4741 aaagcgtgaa ggctgggcaa aaaatcgcca ctatggggag cacggatgcg gcatctgttc
     4801 gcctgcattt ccagattcgt taccgtgcaa cggcaattga tccgctacgt tacttgccgc
     4861 cgcagggcag caagccaaaa tgctgatggc gaattaatca gcagtcagca gcgcggcact
     4921 tgctaaggag agcgtaaggt ttataatgcc ttacgcatct cgaagcgggc gtagttcaat
     4981 ggtagaacga gagcttccca agctctatac gagggttcga ttcccttcgc ccgctccatg
     5041 acacttctta aagtctacta acttactgaa tcctaaggga tacggcttaa cgccttccga
     5101 tagttttact cactgtaacc aacactgtga ctaactttgt tgaagtagtg agcagaccta
     5161 aggtctcagt acatgctcaa gtcaagaacc tacctttatc agcgtaatgg tgtcttttac
     5221 atccgtctta ggatgaagac cactagtcgc ctgaccgctt cgcttccttc tcacaaccgt
     5281 tataaactag cgtcagtatc tttacggacc aaggatagac gtaccgctat ggctcactca
     5341 cggcacatca agtcagcact taaagcaatc catgcagaca accctaacgc ctcttatgag
     5401 gagctacggg agcacctgaa gaccattgtc gagtgggaac ttagtgtcag ccgtgatgac
     5461 ctgaacgacc cggagtccta ccagctttac gttgaccagt acgatgacat taagtccaac
     5521 cttcgggaag ctgtagcgac cgagcgtctg actgtagacc agcatcgcta tattaatgac
     5581 gttatcggtg tgcttaaggc ctgtcaggat agactcaatg gcgatagctc tggcctattg
     5641 tcttaccttg agcctgagac tggcagtcta agaccgtccg tctccctatc tgtattggcg
     5701 gaacctgaag tccctgaacc taaagcactg accctcgcgt ctctcattga gcagtacgag
     5761 caggagaacg cccagaactg gaagccagcg accctaagcg aaaaccgagc gtcacactct
     5821 acgctaatcg agatattcga ctatctggat attcaggatg tcggcaaggc gacccgtgca
     5881 gatatgctca gagttcgtga agtcctccag cagctaccga agaaccgcaa gcaacgcttt
     5941 aagtctatgc cgctgtctga cttgctgaac cgggagtcta agactgactg tctggacgtc
     6001 gttaccatta acaataaata tcttatcaag atggctgctg tgtttaagtg ggcggtacgc
     6061 aacgacttga tagctaagaa cctgaccgaa ggtcttgagc tgaaagtccc tcagcgtaaa
     6121 gcctccgacg ctcgtgatgc gttcagtcct gagcaggtag ggcaactact ggtcgcagct
     6181 aaagcgtact cgcagaagac ctcaggtaag ccataccatt attacgtcac cgctctggct
     6241 gcgattacag gtgcgagact taacgaggtg gcccaacttc aggttaaaga tgtcaggacc
     6301 actgaggctg ggaccgtttt tatccatatc aacgaagacg atagcagcct gccgggtaag
     6361 agtatcaaga acgcccacag tgaccgctgt gtcccgttgg tagatggtgc ttatggtttt
     6421 gtattggctg actttatgtc actcgtggaa gaccgtagaa agactgaggg tgataatgct
     6481 atggtgttca atggcctcaa gctaatgaag aacggctacg gtgagcaggt tagcaagtgg
     6541 tttaaccgaa cgttgctgcc caaagtcctc gctgaccgta gcggcttagc ttttcactcg
     6601 tttagacata cagttgcgac ccagttaaaa caacatgggg tcgagttagc gtatgctcag
     6661 gcgattatgg gtcatagctc agggtctata acttacgacc gttacgcgaa agaggtcgag
     6721 gtggaaacgc tgaaagaaaa attagcggag tcattgtcag taaaaaaaat agacggaaag
     6781 taatcgcata gggatagcgg aatgtgagga cgtaatggtc aaagaatttt tagttagcaa
     6841 agaaaataag ccttcatatg aatgtatgaa tactgctgaa acttgtttgg ggctggctgg
     6901 aatggcttcg gaagattggc aacgtgagca tcattgccgc acaggtataa tatttactgc
     6961 ttttgctatt gaggcaatgt tcatcttcta tagaaaacag gttgaccccg gctacgataa
     7021 gacgcagaaa gagtgcagaa agacaatgca caagaatacg ttaaaattgt gcggtataaa
     7081 caattatatg gggacaaaac cttaccaaat tatcaaagag tgtcttgagg taagagacgc
     7141 tattgctcac ggcgattcgt atacttcgag ctttaatttc tcggcggatc atttggataa
     7201 ccaagacgat attgcaaaag tgtcctggcg gtaaactctg aacaatttcg gggattaact
     7261 gttgagcgcc ttgcaaaata cattgaaatg gcaaagcata ttgaccatga gatttctgat
     7321 aatggttaca ggccttctga aggttacctt ccagcagccg agcgcagaag tctgatgtta
     7381 gcttttggcg tcagtggtgt gtcaatgtgg cccagtgctt gagcgataat acacctcact
     7441 atagaggaat ggacccttaa ctatcactat aggggagaga ccctaagtcc ccaccttaat
     7501 agcagtcacg acccatacac acattcatta agtctcaggc ttccggtagc ggaccgattg
     7561 gaaaccatcc gcagacgcct agtgttaaat caccttggcc tgagaccatt ccttttaacg
     7621 taagctatct tcccttttga caggcgggga gcagtagacc acatccgact ttaagttggc
     7681 tctgtgataa tcgctgcgcg gataactgaa gaaccaagac accattactg aggtctgcca
     7741 tccatttgtg gacaggcgga aactcaaaga ggttagatca gtggtgtctg aggttggagc
     7801 tttgagtctt gaccagcagg ctggaggaga ccatgagttt atcttgttat ggattgtgtt
     7861 atcacaattc caataagaag aactttaagt ccagcctcca ctcccgccaa cccgaagtaa
     7921 aattatggtc gctatgatgt ggtctccatg cttcgggtct tactggaagt actgaacact
     7981 ttcgcgtcaa gttctccgaa gttcattcct gtttgactaa actatcacta tagggaccat
     8041 atctcgcctc tgacctcctg agagacggca agcgagaaac cgtggtccac gctagcaagc
     8101 tagccaccag cctaaggcaa gacagcctct ggtcagcctg tgtgttcttt ggtctcgtga
     8161 cttaagtcta tcaccacaca cgctcggaca tgctgtcgaa ccttcatctc tatggtcatc
     8221 gcagaccact aaccccaatt acctcaagtc attctgtttg cgtcctgttg cctgctggtc
     8281 tccctttacg tgggggcttg agtaggtcag tggggtgcga cttcagggtg caaacaaaag
     8341 gagtacacgt aaatgtccca acaagaaatc agcatcatta acctcgacca gcttgtgtcc
     8401 atgacctcag ttgaaattgc tgagctgaca ggaaaagaac ataaacacgt tttacgagat
     8461 attcgtaata tggtcgaaga gcttaacggg gccaaaactg agcactgctc aactttgagc
     8521 agcgagctta acgggtccaa atttgggctc gttggcgagg aagtatacaa ggacgcgaaa
     8581 ggtgagagcc gaacaatgta tcgtctcgac cgtaagcata ctttcattct ggtcgctggc
     8641 tattccgttc acctccgcgc caagtgttac gaccatattc agacgttaga gcgtcgagtc
     8701 cttcaactgg aagaccagaa gaagcgagca gccattcagt ccgctaaccg ccgaggcgtg
     8761 acttggggcg actactgcaa aacctatggt ctgcccgctc agaagctaat gaccgcactt
     8821 ctccagcatc gtggcctctt tcgtaagaac ccaatcagta acgagtggtc tgtaaatccg
     8881 aagtattccg actgctttcg tatcattaag ccgtcagacc agaagttctc agcaggtggg
     8941 tataacttcc ggttcaacgc taaggggctg gaggtcttcg gtaagcctga gatggttgac
     9001 aagatgcgag gcatccttat tgcattcact ggcaccgacc agcagaagca agagcacctc
     9061 ctgaagctgg cacaatccgg taaggtggag ggaatctaag atgatgattg acccgcgcac
     9121 acctgagggg cgcatgactc tccgttaccg tggctaccgg actgaagtcc tcctcagaga
     9181 gcttggtcta gacccggaag atgagacccg ccagcaccaa tcccgagacg agctaatagc
     9241 ccaactggtc gctatgaaac tgccgcttaa ccgttagtag ctcacctact caatacctaa
     9301 tttaaacacc ttttgagatg accgatgcgg ggagacccgt aggccttact gtgcctgcga
     9361 taccgatagt ctcaactctc aagaggagaa ttacctatga tgttcaacaa caacaactgg
     9421 aagctgtcag tcaccgacat caacctttat gaaaacactg tgagtctgga cggtcagtcc
     9481 tatccgttgt cgctcgctat caagaccctc attccgggct acctgtcagg cttaccg
//
LOCUS       DAZSIO010000148         9513 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZSIO010000148 DAZSIO010000000
VERSION     DAZSIO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.148"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..276
                     /locus_tag="ACHX5T_003258"
     CDS             <1..276
                     /locus_tag="ACHX5T_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR8468330.1"
                     /translation="PAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIV
                     VMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            432..1967
                     /gene="gadC"
                     /locus_tag="ACHX5T_003259"
     CDS             432..1967
                     /gene="gadC"
                     /locus_tag="ACHX5T_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR8468331.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2098..3417
                     /locus_tag="ACHX5T_003260"
     CDS             2098..3417
                     /locus_tag="ACHX5T_003260"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR8468332.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3794..4864
                     /gene="dosC"
                     /locus_tag="ACHX5T_003261"
                     /pseudo
     CDS             3794..4864
                     /gene="dosC"
                     /locus_tag="ACHX5T_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            4864..5481
                     /locus_tag="ACHX5T_003262"
                     /pseudo
     CDS             4864..5481
                     /locus_tag="ACHX5T_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5739..6071
                     /gene="ddpX"
                     /locus_tag="ACHX5T_003263"
                     /pseudo
     CDS             5739..6071
                     /gene="ddpX"
                     /locus_tag="ACHX5T_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            6085..6981
                     /gene="ddpC"
                     /locus_tag="ACHX5T_003264"
     CDS             6085..6981
                     /gene="ddpC"
                     /locus_tag="ACHX5T_003264"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFR8468333.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6978..7952
                     /locus_tag="ACHX5T_003265"
     CDS             6978..7952
                     /locus_tag="ACHX5T_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8468334.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7945..8871
                     /locus_tag="ACHX5T_003266"
                     /pseudo
     CDS             7945..8871
                     /locus_tag="ACHX5T_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
     gene            complement(8927..9358)
                     /gene="osmC"
                     /locus_tag="ACHX5T_003267"
     CDS             complement(8927..9358)
                     /gene="osmC"
                     /locus_tag="ACHX5T_003267"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR8468335.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2321 a         2331 c         2389 g         2472 t
ORIGIN      
        1 ccggcggttt gcttcaaact gaaagatggt gaagatccgg gatacaccct gtacgacctc
       61 tctgaacgtc tgcgtctgcg cggctggcag gttccggcct tcactctcgg cggtgaagcc
      121 accgacatcg tggtgatgcg cattatgtgt cgtcgcggct tcgaaatgga ctttgctgaa
      181 ctgttgctgg aagactacaa agcctccctg aaatatctca gcgatcaccc gaaactgcag
      241 ggtattgccc agcagaacag ctttaaacat acctgataac gtttaacggt aacggtgtcc
      301 cgaaacgaac ccgtttcggg ataatttcca aagtctgttc actggcatta gcaacggaaa
      361 atattgttct gaatacgctt cagaacaaaa caggtgcggt tccgacagga ataccgtttt
      421 agggggataa tatggctaca tcagtacaga caggtaaagc taagcagctc acattactcg
      481 gattctttgc cataacggca tcgatggtaa tggctgttta tgaataccct accttcgcaa
      541 catcgggctt ttcattagtc ttcttcctgc tattaggcgg gattttatgg tttattcccg
      601 tgggactttg tgctgcggaa atggccaccg tcgacggctg ggaagaaggt ggtgtcttcg
      661 cctgggtatc aaatactctg ggtccgagat ggggatttgc agcgatctca tttggctatc
      721 tgcaaatcgc cattggtttt attccgatgc tctatttcgt gttaggggca ctctcctaca
      781 tcctgaaatg gccagcgctg aatgaagacc ccattaccaa aactattgca gcactcatca
      841 ttctttgggc gctggcatta acgcagtttg gtggcacgaa atacacggcg cgaattgcta
      901 aagttggctt cttcgccggt atcctgttac ctgcatttat tttgatcgca ttagcggcta
      961 tttacctgca ctccggtgcc cccgttgcta tcgaaatgga ttcgaagacc ttcttccctg
     1021 acttctctaa agtgggcaca ctggttgtat ttgttgcctt cattttgagt tatatgggcg
     1081 tagaagcttc cgcaactcac gtcaatgaaa tgagcaaccc cgggcgcgac tatccactgg
     1141 ctatgttact gctgatggtg gcggcaatct gcttaagctc tgttggcggt ttgtctattg
     1201 cgatggtcat tccgggtaat gaaatcaacc tctccgcagg ggtaatgcaa acctttaccg
     1261 ttctgatgtc ccatgtggca ccggaaattg agtggacggt tcgcgtgatc tccgcactgc
     1321 tgttgctggg tgttctggcg gaaatcgcct cctggattgt tggtccttct cgcgggatgt
     1381 atgtcacagc gcagaaaaac ctgctgccag cggcattcgc taaaatgaac aaaaatggcg
     1441 taccggtaac gctggtcatt tcgcagctgg tgattacttc tatcgcgttg atcatcctca
     1501 ccaataccgg tggcggtaac aacatgtcct tcctgatcgc actggcgctg acggtggtga
     1561 tttatctgtg tgcttatttc atgctgttta ttggctacat tgtgttggtt cttaaacatc
     1621 ctgacttaaa acgcacattt aatatccctg gtggtaaagg ggtgaaactg gtcgtggcaa
     1681 ttgtcggtct gctgacttca attatggcgt ttattgtttc cttcctgccg ccggataaca
     1741 tccagggtga ttctaccgat atgtatgttg aattactggt tgttagtttc ctggtggtac
     1801 ttgccctgcc ctttattctc tatgctgttc atgatcgtaa aggcaaagcg aataccggcg
     1861 tcactctgga gccaatcaac agtcagaacg caccaaaagg tcacttcttc ctgcacccgc
     1921 gtgcacgttc accacactat attgtgatga atgacaagaa acactaacca tgtctgaatg
     1981 gttataagac aagggagcga taattcatcg ctcccttttt cgtgcttgct gtcgtattga
     2041 cttaaccggg ataagtacga gaatgagcgc acatctgttt accggaaacc agcacatatg
     2101 gatatctgct cccgaaacaa gaaattaacg attagaagac cagcgatact ggttgcattg
     2161 gcacttttac tgtgtagttg taaaagcacg cctccagagt ccatggtgac accaccagca
     2221 ggttcaaagc caccagccac gacgcaacaa tcgtcacaac cgatgcgtgg catctggctg
     2281 gccacggttt ctcgcctcga ctggccaccg gtttcctcgg ttaacattag taatcccacc
     2341 agccgagccc gtgtacaaca acaggcgatg atcgacaaac tggatcatct ccaacgtctc
     2401 ggcataaaca cggtcttttt ccaggtcaag ccggacggta ccgccctgtg gccatcgaaa
     2461 attttgccgt ggtccgatct tatgaccggt aagattggtg aaaatccggg ttacgatccg
     2521 ctgcaattca tgctcgacga agcccacaag cgtgggatga aagtacacgc ctggtttaac
     2581 ccctatcgcg tatcggttaa tacgaagccc ggtactatca gggaactgaa tagcactctg
     2641 tctcaacaac cggcgagcgt ctatgtgcaa caccgtgact ggatcagaac gtctggcgat
     2701 cgctttgtcc tcgacccggg catccctgag gttcaggact ggatcacatc aatagtcgct
     2761 gaagtggttt cccgctatcc ggtagatggc gtgcagtttg acgactattt ctatactgaa
     2821 tcaccgggtt cacggctaaa tgataacgaa acgtaccgta aatacggagg cgcatttgcg
     2881 tcaaaagcag actggcggcg caacaatact cagcagttaa ttgcaaaggt atcacacacc
     2941 attaaaagca ttaagccggg agtcgaattt ggcgttagcc cggcaggcgt gtggcgtaac
     3001 cgatcacacg atccgctcgg ttccgatacc cgaggcgcgg cagcctatga cgaatcctac
     3061 gcagacaccc gtcgatgggt ggaacaagga ttgctggatt acattgctcc ccaaatttac
     3121 tggccgttct cacggagtgc cgcgcgttat gacgtgttgg caaaatggtg ggcggatgtc
     3181 gttaaaccaa ccaggacccg cctgtatatc ggtatcgcct tctataaagt gggtgaacct
     3241 tcaaagatag agccagactg gatgattaac ggcggcgtac cggaactgaa aaagcagctc
     3301 gatcttaacg atgcggtacc agaaattagc ggcaccatct tgttccgtga ggactatctg
     3361 aataaaccgc agactcaaca agcggtcagc tatctgcaaa gtcgttgggg cagttaaact
     3421 accatgctta ctttgtaaca agccggtgct ttacccaccg gcttgttaca ccttgtgaaa
     3481 tatcccacta gtaatcatgc ttacataagt caaattaacc acgcaattaa ccacacttag
     3541 ttaaacagct ataaatccct aataaaaatg tatgaatatg aaaaaccata cgatattcgc
     3601 tgtcaccgaa aaataattac gatgggctat actcttctga agaacagtag cgtagattga
     3661 aactgtctta agtcgcttat ttcttttcag tatatcttca tatttcagga gaatatggtg
     3721 cagttagtcg atctcgcacg ttgcgtttcg ttttgattcc gtctgatttc ccagccttat
     3781 aagggtgtga attatggaga tgtattttaa aagaatgaaa gatgagtgga ccggacttgt
     3841 cgaacaggca gatcctctca ttcgtgctaa agccgcagaa attgcccttg cgcatgctca
     3901 ttatctgagt atcgagtttt atcgaattgt ccgcatcgac ccgcatgccg aagaattctt
     3961 gagtaatgaa caagttgagc ggcagttgaa aagtgcgatg gaacgctgga ttattaacgt
     4021 gctttctgcc caggttgacg atgtcgaaag gctaatacaa atccagcata ccgtcgcgga
     4081 agtgcatgcc cgcataggaa ttccggtaga aattgtcgag atggggtttc gggtgctgaa
     4141 aaaaatcctc tatccggtaa tcttctcttc ggattattcc gccgcagaaa aacttcaggt
     4201 ctaccatttc tcgattaaca gtattgatat cgcgatggaa gtgatgaccc gcgcgtttac
     4261 ctttagtgac agtagtgcct caaaggaaga tgaaaactat cgtatcttct cgttactgga
     4321 aaacgccgaa gaagaaaaag aacggcaaat agcctcaata ctttcatggg aaatagatat
     4381 tatctataaa gtcctgctgg attctgattt aggcagcagt ttgcctttaa gccaggctga
     4441 ttttggcctg tggtttaacc ataaaggtcg acattatttt agcggcattg ccgaagtggg
     4501 ccatatctcc cgtctgattc aggatttcga cggtattttc aatcaaacca tgcgtaacac
     4561 caggattttg aataacagaa gtttgcgggt gaaatttcta ttacagataa gaaataccgt
     4621 atcgcagatt attaccttgc tgcgtgaatt gtttgaagaa gtatcgcgcc acgaagtcgg
     4681 tatggatgta ctgacgaaat tacttaaccg ccgtttcctg ccgactatct tcaaacgcga
     4741 aattgcccat gccaaccgga ccggtacacc gctgtcagtg ctgattattg acgttgataa
     4801 attcaaagag atcaacgata cgtggggcca taacactggc gatgaaattc tgcgtaaagt
     4861 ctcattcctc tcgcagaaga gattggtgaa atcgaaaata ttgggcgctg ggtcatcgcg
     4921 gaagcttgcc gtcagttagc agaatggcgt agccagaata ttcatatccc ggcgttatcc
     4981 gtgaacttgt cggcgctgca ctttcgcagt aatcagctgc ctaatcaggt gtctgatgca
     5041 atgcaagcct ggggtattga cggccaccag ctgacggtgg aaatcacgga aagcatgatg
     5101 atggaacacg ataccgaaat ctttaagcgc attcagatcc tgcgtgatat gggcgtgggt
     5161 ttatcggtag atgattttgg cacgggcttt tccggattat cccgcttagt cagtcttccg
     5221 gtaacggaaa tcaaaattga caaaagcttt gtcgatcgtt gtctgacgga aaaacgcatc
     5281 ctggccttac ttgaagccat taccagcatt gggcaaagcc tcaatttaac cgtcgtggcg
     5341 gaaggggtcg aaaccaaaga gcaatttgag atgctacgta agatccactg tcgcgttatt
     5401 cagggatatt tcttttcccg cccccttccc gccgaagaaa ttccaggctg gatgagcagc
     5461 gtgttaccgc tgaaaatctg acaaattcct ctcgcccgca ctcgcgggtt ttcctttaag
     5521 gtgacactgt cacttaaata ctgtgatttc agctacgatt ccgggagatt ccttccttaa
     5581 ctttccctct tgcaccaaaa ctgcactacg ctgcacaaat gttgatcaaa agttaaaaaa
     5641 tataacttta agccagcatt ctggcgctta tcccggcatg gcatgagatc tgcataagcg
     5701 gaaagcgcag caatttttgt cttatacagg aaccgtttat gtcggatacc accgaactgg
     5761 ttgatttagc cgtgatcttc cctgatctgg agatcgaatt gaaatatgcc tgcgttgata
     5821 acatcacagg taaagctatt tatcagcaag cgcgttgtct gctacataaa gatgcgatta
     5881 ccgcgctggc gaaaagtatc agtatcgccc agctgtcagg cttacaactg gtgatttacg
     5941 atgcgtatcg cccacaacaa gcacaggcga tgttgtggca agcctgccca gacccgcaat
     6001 atgttgttga tgtgacggtc ggttctaatc acagccgtgg cacggcgctc gacctgacgc
     6061 ttcgtgatga cgtggaggtg gtgaatgatg ctaagcgagg aaacgtccgc cgtacgccca
     6121 caaaaacaaa cgcgatttaa cggtgcaaaa ctggtatgga tgctgaaagg cagtccgctc
     6181 accgtgaccg gcgcagtcat cattgtatta atgcgactga tgatgatttt ttcaccgtgg
     6241 ctggcgacgc atgatcccaa cgccattgat ttaaccgccc gccttttgcc gccttctgcg
     6301 gcgcactggt ttggcaccga tgaagtggga cgcgatctgt ttagccgcgt actggtcggc
     6361 agtcagcaat caattctcgc cggattagtg gtggtcgcca ttgcgggtat gattggttcg
     6421 ctactcggat gtctatccgg tgtgcttggc ggacgcgcag acgccatcat catgcgcatc
     6481 atggacatta tgctgtcgat tccttcgttg gtactgacaa tggcactggc tgccgctctc
     6541 gggccgagtt tatttaacgc catgctggcg attgctattg tgcgaattcc cttttatgtg
     6601 cgcttggcgc gggggcaagc attagttgta cgccagtata cctatgttca ggcggcgaaa
     6661 acctttggcg cgtctcgttg gcatctgatc aactggcata ttttacgtaa ctccctaccg
     6721 ccgctgatcg tgcaggcatc gctggatatc ggtagcgcga ttttaatggc cgccacgttg
     6781 ggatttattg gcctgggtgc tcaacaaccg agtgctgaat ggggcgcgat ggtagcgaat
     6841 ggtcgcaact atgtactcga tcagtggtgg tattgcgcat ttccgggggc agcgattttg
     6901 cttacctccg tcgggtttaa tctctttggt gatggtattc gcgatctgct tgacccgaaa
     6961 gcaggaggaa agcagtcatg acccaacccg ttctggacat tcaacagctg catttgagtt
     7021 tccccggttt taacggtgat gttcacgcgc tcaacaatgt gtccttgcag attaaccgcg
     7081 gcgaaattgt cggtctggtg ggagaatccg gctcaggtaa atcagtcacc gcaatgctga
     7141 ttatgcgtct gctaccgacg ggcagttatt gcgtacatcg ggggcagatt tcactgctgg
     7201 gagaagatgt tcttaacgct cgggaaaaac agcttcgtca gtggcgcggc gcacgagtgg
     7261 cgatgatctt tcaggaaccg atgaccgccc ttaatccgac acgtcgaata ggtcttcaga
     7321 tgatggacgt aatccgccat catcaaccaa taagtcgtcg ggaagccaga gctaaagcga
     7381 ttgcactgct ggaagagatg caaatcccgg atgccgtgga agttatgtcg cgctatccgt
     7441 ttgagctttc aggtggtatg cgccagcggg taatgattgc gccggcattc tcctgcgagc
     7501 cgcaattgat tattgccggc gaaccgacga cggcgctgga cgtcacagta cagttgcagg
     7561 tactgcgttt gcttaaacat aaagcacgcg ccagtggaac tgcggtactg ttcatcagcc
     7621 atgatatggc cgtggtgtcg caactgtgcg atagcgttta cgtgatgtat gccggaagcg
     7681 taattgaaag cggcgggacg gcagacgtta tccatcatcc ccggcatccg tataccattg
     7741 gtttgctgca atgcgcacca gaacatggag taccacgcca gccattaccc gccattccag
     7801 ggacggtacc aaacctcacc catttgcctg acggctgcgc ttttcgcgat cgttgctatg
     7861 cggcaggtgc acagtgtgcg ctgacagcgt gtggtgacaa caaccagcac tgcacctgtt
     7921 ggtatccgca gcaggaggtc ataagtgtct gacacgttat taacgttacg cgacgtccat
     7981 atcaattttc cggcccgtaa aaactggctt ggcaaatcta cggaacatgt tcatgccatt
     8041 aatggtattg atttacagat ccgccgtggt gaaaccttag ggatcgtcgg cgagtcaggc
     8101 tgcggcaaaa gcaccctcgc acagctttta atgggcatgc tgcaaccgag ccacgggcag
     8161 tacatccgtt caggctcaca acgcattatg cagatggtgt ttcaggaccc gctctcttcg
     8221 cttaatccgc gcttaccggt gtggcgcatc atcacagaat cgctctggat agctaagcgt
     8281 agtagtgaac aacagcggcg agcgttggca gaggagttgg ctgtgcaggt gggtattcgt
     8341 ccggagtatc tcgaccgcct gcctcatgcg ttctccggcg ggcagcggca acgcatcgcc
     8401 attgccagag cactctcttc gcagcctgac gtgattgtgc ttgatgagcc aacctcggcg
     8461 ctggatatct ccgtgcaggc gcagatcctc aatttgctgg taacgctaca ggaaaatcac
     8521 gggctgactt atgtgctgat ttcacacaat gtctcggtga tacgtcatat gagcgatcgg
     8581 gtggcggtga tgtatctcgg gcagattgta gaactgggcg acgcgcaaca ggtgttgact
     8641 gcttcggcac atccatacac ccgattattg ctggattccc tccccgccat tgataaaccg
     8701 ctggaggaag aatgagcatt acgtaaaacg gatctgccgg gaaaccgcac gttgccgcaa
     8761 ggctgttttt tctacgaacg ttgccctctg gcaacccacg gatgtgaagt ccggcaatca
     8821 ttagcaataa gggaggacgg acgtgacctc cggtgctggc gggcgctgta gagatgctaa
     8881 caacgcatca ggcattcacg cgcctgacgc gtcatccggc aatgctttac gatttcaact
     8941 ggtaatccag cgtaatttcc gctttcagta cctgagagac cgggcatcct gcttttgctt
     9001 tctggatgat gccgtcaaag gtagaggcat caatacccgg caccgcaact tcactcttca
     9061 gtgcgatttt cgtaatcgca aaaccggcat ccactttatc cagcgacaca tcggcggtgg
     9121 tatcaattga tgttggcgtg aatcccgctt cccccagcat taatgaaagc gccattgaga
     9181 aacatgcggc atgcgctgcg ccaatcagtt cttcagggtt ggttcctttt tcgccttcaa
     9241 aacgcgtgtt aaatccatac ggctgttggt tcagcacgcc gctctcggtg gatactgttc
     9301 ccttcccgcg tttgatatcg ccttcccagt gtgcctgacc tttcttatgg attgtcattg
     9361 ttgctctcct gtgggcttaa aaaggaaatt atagactacg tgagaaacag cacgaggata
     9421 agcaggatat tccgaataaa atacggcgaa aatattactt ctgcacttaa attaaattcc
     9481 cacaatagat aaccgggaat atatatctca aga
//
LOCUS       DAZSIO010000149         9438 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZSIO010000149 DAZSIO010000000
VERSION     DAZSIO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.149"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(16..998)
                     /gene="kdgT"
                     /locus_tag="ACHX5T_003268"
                     /pseudo
     CDS             complement(16..998)
                     /gene="kdgT"
                     /locus_tag="ACHX5T_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="ACHX5T_003269"
     CDS             complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="ACHX5T_003269"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR8468336.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2163..3197
                     /gene="rhaT"
                     /locus_tag="ACHX5T_003270"
                     /pseudo
     CDS             2163..3197
                     /gene="rhaT"
                     /locus_tag="ACHX5T_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3194..4042)
                     /gene="rhaR"
                     /locus_tag="ACHX5T_003271"
     CDS             complement(3194..4042)
                     /gene="rhaR"
                     /locus_tag="ACHX5T_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFR8468337.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="ACHX5T_003272"
     CDS             complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="ACHX5T_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR8468338.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5240..6709
                     /gene="rhaB"
                     /locus_tag="ACHX5T_003273"
     CDS             5240..6709
                     /gene="rhaB"
                     /locus_tag="ACHX5T_003273"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR8468339.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6706..7965
                     /gene="rhaA"
                     /locus_tag="ACHX5T_003274"
     CDS             6706..7965
                     /gene="rhaA"
                     /locus_tag="ACHX5T_003274"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR8468340.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8270..9031
                     /gene="rhaD"
                     /locus_tag="ACHX5T_003275"
     CDS             8270..9031
                     /gene="rhaD"
                     /locus_tag="ACHX5T_003275"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR8468341.1"
                     /translation="MKGWDERNGGNLTLRLDDADIALYHDNFHPQPRYIPLSQPMPLL
                     ANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGYHILWGLTNEAVPTSELPAHFL
                     SHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAVFTRQLWEGSTECLVVFPDGVG
                     ILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSGPTLDEAFGLIDTAEKSAQVLV
                     KVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            9041..9355
                     /gene="rhaM"
                     /locus_tag="ACHX5T_003276"
     CDS             9041..9355
                     /gene="rhaM"
                     /locus_tag="ACHX5T_003276"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR8468342.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         2293 a         2473 c         2471 g         2201 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc cagcttcttc tttcgtaccg
      601 tactgctgca tgatggaagc gtaaagtccg ccgttggtca tatccatcgc cgccaccagc
      661 gccagcgttg aaagtccggc aaagaatcca acttcaacac catgttccgg aataatgcgc
      721 gaggcaattg ccgcaaccac ccacgcgacg gcaattttag ttaccaccag cgtaccggat
      781 ttacgcagta ccgttcccgt cgcgcttaat tttattgacg cccccatgca aaaaaaccac
      841 accgccagaa tgggcaccgt accggtaatc atcccgttgg tgaatgatcc aaaatatttc
      901 cccgcccccg gcgagaaggt gtggcacagt gcgccaagga atagcgggac gagcatcatc
      961 ccccccggga ttttctcaat cgagcgtttt atctgcattt ccatcacctg tcggcatatt
     1021 tttaagggct gatgcgtaat tcctcaatgg agcatcagtg ttgatggaaa cgaagataga
     1081 cattcattta atcaataaaa gtgatcaaaa tcacatcaat tgaaacgctg ttttattttt
     1141 ataggaccat tgtttttatt ttggaattga tcacaaaaaa accaccgccg ttggcgatgg
     1201 ttcatttttt aagctgatat gcggcctata cgcctcattg cagcaggcgg caaataatta
     1261 ttttttcgcc gcaaaacgtg ccgctgcttc gtcccagttc accacgttcc agaactcttt
     1321 gatgtagtcc ggacggcggt tctggaattt caggtagtaa gcatgttccc acacatccag
     1381 gcccagaatc gggaagccgg aagcgccaga aatagcttca cccatcagcg gagaatcctg
     1441 gttagcagta gaaaccaccg ccagtttatc gcctttcagc accagccatg cccagccgga
     1501 accaaagcgg gaagctgccg ctttttcaaa ttctgctttg aagttatcaa cggagccgaa
     1561 gtcacgttcg atagccgctt tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt
     1621 ccagaacagg ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc
     1681 tgctggcagc tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg caaattctgg
     1741 caggctttcc agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg
     1801 gatttccatg gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat acggcaggga
     1861 tggcagggta tagctcatat tcatctccag tattgtcggg cggccgattg ttaatgccgc
     1921 gtaagcagtt ggttcattat agttaattaa atgatattga aaatgattat caatgccgta
     1981 cttttcgtaa gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact
     2041 taatgccgtg attgccagta aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag
     2101 cacgattttt acactcatct cgtcggagat gtgaagcgac gaaaaatgat gaggataaga
     2161 agatgagtaa cgcgattacg atggggatat tttggcattt gatcggcgcg gccagtgcag
     2221 cctgttttta cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag
     2281 tcggtgggat tgtttcgtgg attattctgc cgtgggccat cagcgccctg ttactaccgg
     2341 atttctgggc gtattacagc tcgtttagtc tctctacgct gctgcctgtt tttctgttcg
     2401 gcgctatgtg ggggatcggt aatatcaact atggcctgac catgcgttat ctcggcatgt
     2461 cgatgggaat tggcatcgcc attggcatta cgttgattgt cggtacgctg atgacgccaa
     2521 ttatcaacgg caatttcgat gtgctgatta acaccgaagg cggacgcatg acgttactcg
     2581 gcgttctggt ggcgctgatt ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc
     2641 gcaagatggg cattaaagcc gaagagttca atctgaaaaa agggctggtg ctggcggtga
     2701 tgtgcggcat tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg
     2761 aagccgctgc cgcacttggc gtcgatccac tgtatgttgc tctgccaagc tatgttgtca
     2821 tcatgggcgg cggcgcgatc attaacctcg gtttctgttt tattcgtctg gcaaaagtga
     2881 aggatttgtc gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt
     2941 tactctcggc actgggcggg ttgatgtggt atctgcaatt ctttttctat gcctagggcc
     3001 acgcccgcat tccggcgcag tatgactaca tcagttggat gctgcatatg agtttctatg
     3061 tattgtgcgg cggtatcgtc gggctggtgc tgaaagagtg gaacaataca ggacgccgtc
     3121 cggtaacggt gttgagcctc ggttgtgtgg tgattattgt cgccgctaac atcgtcggca
     3181 tcggcatggc gaattaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc
     3241 cggtttcccg ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg
     3301 aaatatcact gattaacagg cggctatgct ggagaagata ttgcgcatga cacactctga
     3361 cctgccgcag atattgattg atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa
     3421 cgcgctcact gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc
     3481 tagccgccag ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg
     3541 aatcactggt gtaacgatgg cgattcagca acatcaccaa ctgcccgaac agcaactcag
     3601 ccatttcgtt agcaaacggc acatgctgac tactttcatg ctcaagctga ccgataacct
     3661 gccgcgcctg cgccatcccc acgctaccta agcgccagtg tggttgccct gcgctggcgc
     3721 taaatcccgg aatcgctccc tgccagtcaa gattcagctt cagccgctcc gggcaataaa
     3781 taatattctg caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa
     3841 tataaaagag atcgccacgg gtaatgcgat aaggacgatc gttgagtaca tgcagaccat
     3901 taccgcgcca gacaatcacc agctcacaaa aatcatgtgt atgttcggca aagacatctt
     3961 gcggataacg gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga
     4021 gaagttttaa ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg
     4081 caatatggcg tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc
     4141 tggcgaatat cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta
     4201 ctgtcgctga atccacagcg ataggcgatg tcagtgacgc tggcctcgct gtggcgtagt
     4261 agatgtcggg ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt
     4321 tgctgcttaa gctgccgatg tagcgtacgc agtgaaagag aaaattgatc cgccacggca
     4381 tcccaattca cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt
     4441 gatgcgctgt tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc
     4501 ataaacaaga tctcgcgact ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc
     4561 atctgtgcaa ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg ccagtgagac
     4621 ggatactgcc catccagctc ttgtggcagc aactgattca gcccggcgag aaactgaaat
     4681 cgatccggcg agcgatacag cacattggtc agacacagat tatcggtatg ttcatacaga
     4741 tgccgatcat gatcgcgtac gaaacagacc gtgccaccgg tgatggtata gggctgccca
     4801 ttaaacacat gaatacccgt gccatgttcg acaatcacaa tttcatgaaa atcatgatga
     4861 tgttcaggaa aatccgcctg cgggagccgg ggttctatcg ccacggacgc gttacaagac
     4921 ggaaaaaaat ccacactatg taatacggtc atactggcct cctgatgtcg tcaacacggc
     4981 gaaatagtaa tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa
     5041 aacgtcgatt tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac
     5101 cacaattcag caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca
     5161 ttttcctgtc agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga
     5221 aattcagcag gatcacatta tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc
     5281 cagtgggcgc gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga
     5341 aatccatcgt tttaacaatg ggctgcatag ccaaaacggt tatgtcacct gggatgtgga
     5401 tagcctggaa agtgccattc gccttggatt aaacaaggtg tgcgaggaag ggattcgtat
     5461 cgatagcatt gggattgata cctggggcgt ggactttgtg ctgctcgacc aacagggtca
     5521 gcgtgtgggc ctgcccgttg cttatcgcga tagccgcacc aatggcctga tgccgcaggc
     5581 acaacagcaa ctcggcaaac gcgatattta tcagcgtagc ggcatccagt ttctaccctt
     5641 caatacgctt tatcagttgc gtgcgctgac ggagcaacaa cctgaactta ttccacacat
     5701 tgctcacgct ctgctgatgc cggattactt cagttatcgc ctgaccggca agatgaactg
     5761 ggaatatacc aatgccacca ccacacaact ggtcaatatc aatagcgacg actgggacga
     5821 gtcgctactg gcgtggagcg gggccaacaa agcctggttt ggtcgcccga cgcatccggg
     5881 taatgtcatt gggcactgga tttgcccgca gggtaatgag attccggtgg tcgccgttgc
     5941 cagccatgat accgccagcg cggttatcgc ctcgccgtta aacggttcac gcgccgctta
     6001 tctctcttct ggcacctggt cattgatggg cttcgaaagc cagacgccat ttaccaatga
     6061 cacggcgctg gcagccaaca tcaccaatga aggcggggcg gaaggtcgct atcgggtgct
     6121 gaaaaatatt atgggcttat ggctgcttca gcgagtgctt caggaacggc aaatcaacga
     6181 tctcccggcg cttatcgccg cgacacaggc acttccggcc tgccgcttca tcatcaatcc
     6241 caatgacgat cgctttatta atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg
     6301 ggaaacggcg caaccgatcc cgggaagtga tgctgaactg gcgcgctgta ttttcgacag
     6361 tctggcgctg ctgtatgccg atgtgttgca tgagctggcg cagctgcgcg gtgaagattt
     6421 ctcgcaactg catattgtcg gcggcggctg ccagaacacg ctgctcaacc agctatgcgc
     6481 cgatgcctgc ggtattcggg tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat
     6541 cggcatcgag ttaatgacgc tggatgaact caacaatgtg gatgatttcc gtcaggtcgt
     6601 cagcaccacc gcgaatctga ccacctttac ccctaatcct gacagtgaaa ttgcccacta
     6661 tgtggcgcag attcactcta cacgacagac aaaggagctt tgcgcatgac cactcaactg
     6721 gaacaggcct gggagctggc gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag
     6781 gcgctgcgcc aacttgatcg tttacccgtt tcaatgcact gctggcaggg cgatgatgtt
     6841 tccggttttg aaaacccgga aggttcgctg accgggggga ttcaggccac aggcaattat
     6901 ccgggcaaag cgcgtaatgc cagtgagcta cgtgccgatc tggaacaggc tatgcggctg
     6961 attccggggc cgaaacggct taatttacat gccatctatc tggaatcaga tacgccagtc
     7021 tcgcgcgacc agatcaaacc agagcacttc aaaaactggg ttgaatgggc gaaagccaat
     7081 cagctcggtc tggattttaa cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt
     7141 acgctctcgc atcccgacga cagcattcgc cagttctgga ttgatcactg caaagccagc
     7201 cgtcgcgttt cggcctattt tggcgagcaa ctcggcacac catcggtgat gaacatctgg
     7261 atcccggatg gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg
     7321 gcagcactgg atgaggtgat cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt
     7381 gagagcaaat tgtttggcat tggcgcagag agctacacgg ttggctccaa tgagttttac
     7441 atggggtatg ccaccagccg ccagactgcg ctgtgcctgg acgccgggca cttccacccg
     7501 actgaagtga tttccgacaa gatttccgcc gccatgctgt atgtaccgca gttgctgctg
     7561 cacgtcagcc gtccggttcg ctgggacagc gatcacgtag tgctgctgga tgatgaaacc
     7621 caggcaattg ccagtgagat tgtgcgtcac gatctgtttg accgggtgca tatcggcctt
     7681 gacttcttcg atgcctctat caaccgcatt gccgcgtggg tcattggtac acgcaatatg
     7741 aaaaaagccc tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa gctggaagcg
     7801 gcgggcgatt acactgcgcg tctggcactg ctggaagagc agaaatcgtt gccgcggcag
     7861 gcggtctggg aaatgtattg ccaacatcac gatacgccag caggtagcga atggctggag
     7921 agcgtgcggg cttatgagaa agcgattttg agccagcgtg ggtaaacact gccggatgcg
     7981 gcacgagcgc cttatccggc ctacgggtcg aatgtccatg cgggctgtca ccctcacccc
     8041 ggccctctcc ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa cggagctgaa
     8101 caccgctccg gacagtcccc tcgcccctac ggggagaggg ttagggtgag gggaacaagc
     8161 cgcaccatgt ctaaacaagg caacctaatt tacaggaaca cagaacatgc aaaacattac
     8221 ccagtcctgg tttgtccagg gaatgatcaa agccaccacc gatgcctagc tgaaaggctg
     8281 ggacgagcgc aacggcggca acctgacgct gcgcctggat gacgccgata tcgcgcttta
     8341 tcacgacaac ttccacccac aaccgcgcta tattccgctc agccaaccca tgcctttact
     8401 ggcaaatacg ccgtttattg tcaccggctc cggcaaattc ttccgtaacg tccagctcga
     8461 tcctgcagct aacttaggcg tcgtaaaagt cgacagcgat ggcgcgggct accatattct
     8521 ctgggggtta accaacgaag ccgttccaac ttctgaactt ccggctcact ttctctccca
     8581 ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg atcatgcact gccacgccac
     8641 caacctgatc gccctcacct atgtactgga aaacgacacc gcggtcttta ctcgccaact
     8701 gtgggaaggc agcaccgaat gtctggtagt attcccggat ggcgttggca ttctgccgtg
     8761 gatggtgccc ggcacggacg aaatcggcca ggcgaccgca caagagatgc aaaaacattc
     8821 gctggtgttg tggcccttcc acggtgtgtt cggttccgga ccgacgctgg atgaagcctt
     8881 tggtttaatc gacaccgcag aaaaatcagc acaagtatta gtgaaggttt attcgatggg
     8941 cggcatgaaa cagaccatca gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt
     9001 tacaccacta gccagtgcgc tggcgctgta aggagcaaac atgatccgca aagcctttgt
     9061 catgcaggta aaccccgacg cccacgaaga gtatcagcgt cggcataatc ccatctggcc
     9121 ggaactggaa gcagtgttga aatctcacgg tgtgcataac tacgccattt acctcgacaa
     9181 agcgcgtaat ctgctgtttg ccatggtaga gattgaatct gaagaacggt ggaatgcggt
     9241 agccagtact gatgtttgcc aacgttggtg gaaatatatg accgatgtta tgcccgctaa
     9301 cccagataac agcccggtga gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc
     9361 caatgtgccg gagatgcctc cggcacaata ttttaaattt cgatttgaaa aattaaacat
     9421 attacatctc ccggtaag
//
LOCUS       DAZSIO010000150         9319 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZSIO010000150 DAZSIO010000000
VERSION     DAZSIO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.150"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="ACHX5T_003277"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="ACHX5T_003277"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR8468343.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="ACHX5T_003278"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="ACHX5T_003278"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFR8468344.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="ACHX5T_003279"
     CDS             3115..3399
                     /locus_tag="ACHX5T_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR8468345.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="ACHX5T_003280"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="ACHX5T_003280"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR8468346.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="ACHX5T_003281"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="ACHX5T_003281"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR8468347.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="ACHX5T_003282"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="ACHX5T_003282"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR8468348.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQSLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQSHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8449..>9319
                     /gene="yddG"
                     /locus_tag="ACHX5T_003283"
     CDS             8449..>9319
                     /gene="yddG"
                     /locus_tag="ACHX5T_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR8468349.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLAT"
BASE COUNT         2218 a         2467 c         2374 g         2260 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg aggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ctataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggctctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcga ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8461 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8521 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8581 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8641 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct atctggcgct ttccttaggg
     8701 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8761 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8821 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8881 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8941 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9001 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9061 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9121 accttaggat ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9181 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9241 agcgccccgc tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg
     9301 ctctgctggc tggcgacac
//
LOCUS       DAZSIO010000151         9153 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZSIO010000151 DAZSIO010000000
VERSION     DAZSIO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.151"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..923
                     /locus_tag="ACHX5T_003284"
     CDS             <1..923
                     /locus_tag="ACHX5T_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8468350.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="ACHX5T_003285"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="ACHX5T_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR8468351.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACHX5T_003286"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACHX5T_003286"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR8468352.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSNSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="ACHX5T_003287"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="ACHX5T_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR8468353.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="ACHX5T_003288"
     CDS             4446..4805
                     /locus_tag="ACHX5T_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR8468354.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="ACHX5T_003289"
     CDS             complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="ACHX5T_003289"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR8468355.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6059..6249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6314..8158
                     /gene="btuB"
                     /locus_tag="ACHX5T_003290"
     CDS             6314..8158
                     /gene="btuB"
                     /locus_tag="ACHX5T_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR8468356.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8103..8960
                     /gene="murI"
                     /locus_tag="ACHX5T_003291"
     CDS             8103..8960
                     /gene="murI"
                     /locus_tag="ACHX5T_003291"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR8468357.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2091 a         2210 c         2473 g         2379 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcgcc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccatgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatttgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcatcgcgca gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc
     5581 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
     5641 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt
     5701 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
     5761 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga
     5821 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
     5881 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
     5941 gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg
     6001 tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct
     6061 gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga
     6121 cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga
     6181 agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt
     6241 cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg
     6301 tggatgcttt acaatgatta aaaaagcttc gctgctgacg gcgtgttccg tcacggcatt
     6361 ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga
     6421 acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg
     6481 ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca
     6541 aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt
     6601 gctggtgtta attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct
     6661 tagccagttc cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc
     6721 tgtttatggt tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc
     6781 cggaacggaa atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac
     6841 gcagcaacaa ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca
     6901 tggttatgat gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg
     6961 ttttttaagt aaaacgcttt atggggcgct ggagcataac tttactgatg cctggagcgg
     7021 ctttgttcgc ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg
     7081 ttcaccgttg ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta
     7141 taacggcgaa ctgattaaat cacaactcat taccagctat agccatagca aagattacaa
     7201 ctacgatccc cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata
     7261 caccgtccag tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga
     7321 ctggcagaaa cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg
     7381 taataccggc atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc
     7441 agcacgcagc gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg
     7501 ttgggaattc atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcact
     7561 aaatctgggg caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa
     7621 acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata
     7681 tcgtaacgat gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga
     7741 agggaaagcg cggattaagg gtgtcgaggc gacagccaat tttgataccg gaccactgac
     7801 gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt
     7861 acgccgtgct aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg
     7921 gggtattact tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta
     7981 tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac
     8041 ctctcacctg acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt
     8101 ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac
     8161 cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga
     8221 tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt
     8281 atggcgaaaa aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc
     8341 aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac
     8401 ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac
     8461 ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta
     8521 aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc
     8581 tgggctcggc agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc
     8641 tggatgcact aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg
     8701 ttgtattggg ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag
     8761 agggaacccg gctggtggat tctggcgcag cgattgctcg ccgaacggcc tggttgttag
     8821 aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga
     8881 cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa
     8941 aactggcagt tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt attttaaatt
     9001 tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc
     9061 aaacacgccg acgggtcagc ggggttctcc tgagaattcc ggcagagaaa gcaaaaataa
     9121 atgcttgact ctgtagcggg aaagcgtatt atg
//
LOCUS       DAZSIO010000152         9029 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZSIO010000152 DAZSIO010000000
VERSION     DAZSIO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.152"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACHX5T_003292"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACHX5T_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR8468358.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACHX5T_003293"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACHX5T_003293"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR8468359.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACHX5T_003294"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACHX5T_003294"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR8468360.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACHX5T_003295"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACHX5T_003295"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR8468361.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVQ
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4466..5590
                     /gene="dprA"
                     /locus_tag="ACHX5T_003296"
     CDS             4466..5590
                     /gene="dprA"
                     /locus_tag="ACHX5T_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR8468362.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5562..6035
                     /gene="smg"
                     /locus_tag="ACHX5T_003297"
     CDS             5562..6035
                     /gene="smg"
                     /locus_tag="ACHX5T_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR8468363.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6064..6606
                     /locus_tag="ACHX5T_003298"
     CDS             6064..6606
                     /locus_tag="ACHX5T_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR8468364.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6611..7183
                     /gene="tsaC"
                     /locus_tag="ACHX5T_003299"
     CDS             6611..7183
                     /gene="tsaC"
                     /locus_tag="ACHX5T_003299"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR8468365.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7188..8006
                     /gene="aroE"
                     /locus_tag="ACHX5T_003300"
     CDS             7188..8006
                     /gene="aroE"
                     /locus_tag="ACHX5T_003300"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR8468366.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8003..8260
                     /locus_tag="ACHX5T_003301"
     CDS             8003..8260
                     /locus_tag="ACHX5T_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR8468367.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8236..8790)
                     /locus_tag="ACHX5T_003302"
     CDS             complement(8236..8790)
                     /locus_tag="ACHX5T_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR8468368.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2259 a         2191 c         2251 g         2328 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gtgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacaacgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgctgca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag
     4441 gcttgcttat ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag
     4501 catcagcagc ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc
     4561 gcatattgat gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt
     4621 tctttcattt ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca
     4681 tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta
     4741 ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt
     4801 agtggggagt cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac
     4861 tctggcgacg tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt
     4921 gcataaagcg gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa
     4981 taccattcat ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc
     5041 tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa
     5101 tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg
     5161 ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg
     5221 tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct
     5281 tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga
     5341 cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga
     5401 gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg
     5461 ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc
     5521 agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt
     5581 atttgtttga aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg
     5641 aacaggatct taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc
     5701 tggaaaaact tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc
     5761 ctctctccat gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg
     5821 ggtttctgct tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga
     5881 tagagcgagt gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga
     5941 tcctgatggt gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat
     6001 tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt
     6061 gttatggcaa aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg
     6121 gctgaactgg ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg
     6181 gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg
     6241 gaggggcagg tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt
     6301 atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg
     6361 gacgaaacag caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc
     6421 cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac
     6481 ttcaaaccaa tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa
     6541 accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg
     6601 gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct
     6661 caatgaaaaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc
     6721 tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg
     6781 gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt
     6841 gactgacgtg cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt
     6901 tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg
     6961 agtcaccgac catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc
     7021 taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca
     7081 atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga
     7141 aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg
     7201 ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc
     7261 agcaactgaa tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca
     7321 acacactgaa cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta
     7381 aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag
     7441 ttaataccct caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag
     7501 gcttgttaag cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta
     7561 tcggcgctgg tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg
     7621 tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca
     7681 ctggcagtat tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta
     7741 ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc
     7801 atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat
     7861 ggtgtgagca gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg
     7921 cggctcatgc ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc
     7981 aattgcagga ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg
     8041 ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg
     8101 cgcgatctct ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt
     8161 agcgagtttt cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga
     8221 acaaagtgaa gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca
     8281 tttcacgtaa ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg
     8341 gatctgtttg acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg
     8401 gaccagacta cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat
     8461 tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg
     8521 accaacagtg acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt
     8581 atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata
     8641 atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac
     8701 gtcaccaatc acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag
     8761 atcgcggtat gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat
     8821 gactttgggg gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt
     8881 gtgtgcgatt gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat
     8941 acttgtgcaa aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca
     9001 cttcacagga tggtcggaac aacgaagag
//
LOCUS       DAZSIO010000153         8872 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZSIO010000153 DAZSIO010000000
VERSION     DAZSIO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8872
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.153"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..439
                     /locus_tag="ACHX5T_003303"
     CDS             <1..439
                     /locus_tag="ACHX5T_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR8468369.1"
                     /translation="QKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQ
                     FAVQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYA
                     KAKLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="ACHX5T_003304"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="ACHX5T_003304"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR8468370.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="ACHX5T_003305"
     CDS             complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="ACHX5T_003305"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR8468371.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="ACHX5T_003306"
     CDS             complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="ACHX5T_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR8468372.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4441..5148)
                     /locus_tag="ACHX5T_003307"
     CDS             complement(4441..5148)
                     /locus_tag="ACHX5T_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFR8468373.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPGFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="ACHX5T_003308"
     CDS             complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="ACHX5T_003308"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR8468374.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6006..6209)
                     /locus_tag="ACHX5T_003309"
     CDS             complement(6006..6209)
                     /locus_tag="ACHX5T_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR8468375.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6368..7876
                     /locus_tag="ACHX5T_003310"
                     /pseudo
     CDS             6368..7876
                     /locus_tag="ACHX5T_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7873..8769
                     /locus_tag="ACHX5T_003311"
                     /pseudo
     CDS             7873..8769
                     /locus_tag="ACHX5T_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            complement(8785..>8872)
                     /locus_tag="ACHX5T_003312"
     CDS             complement(8785..>8872)
                     /locus_tag="ACHX5T_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR8468376.1"
                     /translation="VRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         2062 a         2416 c         2273 g         2121 t
ORIGIN      
        1 gcagaaggtg gcgttaaatc tcgcttcagg tgaagggaat ttctttatcc gtcctggcgg
       61 cgtgttttat gtcgcgggag ataaagtcgg catcgttcgt ctggatgctt ttaaagccag
      121 taaagatatt cagtttgcgg tgcagtcagg accaatgttg atggaaaacg gtgtaattaa
      181 tccgcgtatt catcccaacg tcgcctcacg taaaattcgt aacggtgttg ggattaataa
      241 acatgggaat gccgtgtttt tgttgagcca gcaggcgaca aatttttatg attttgcctg
      301 ttatgccaaa gcgaaactga acgttgagca gctgctttat cttggtggca cgatttcaca
      361 tatgtatatg aaaggcgggg caatcccgtg gcaacgctat ccttttgtca ccatgatttc
      421 cgtcgagcgg aaaggttaga atgatagctt tgtgtcagat actaaatatg ctgctgatgc
      481 cggatgcggc gtaaacgcct tatccggcct acatgacgtt gcaatttatt gaatctgaat
      541 gattttttag gccaaataag gtgcgcagca ccgcatccgg caacgttaca ccgactccag
      601 ccgggcgtaa gctgccacca gccatttgat tccctggccc tggaatgcca cctgcaaacg
      661 gctatgttca ccgctgcctt ccatattgac gatggtgcct tcaccaaact tagcgtggcg
      721 tacacgctgg ccgagcttgt agccgctgtc gttctcgacc atcggcgtac ccatccgctg
      781 atggctgacc gggcggctta ccgtggcgcg caggcgcacc tcttccacac actcttccgg
      841 caactcgcca ataaagcgcg acgggcgatg gtaaacctct ttgccataca gacggcgagt
      901 ttccgcgtag gtcagcgtca gtttctgcat cgcgcgggtt acgccaacgt aggccagacg
      961 gcgttcttct tccagacgcc cgccttcatc cagcgacatc tggcttggga acatgccctc
     1021 ttccataccg acgataaaca cctgcgggaa ctccagacct ttcgccgagt gcatggtcat
     1081 cagttgtacc gcatcctgcc aggtatccgc ctgcccttcg cctgcttcca gcgccgcatg
     1141 ggataggaac gcctgcagcg gcattaaatc ttcgtcttct tcgttgtagc tgaactggcg
     1201 cgttgccgtc accagttcct ctaagttttc gatacgcgtc tgaccttttt cgcccttctc
     1261 ctgctcgtac atggtacgca ggccggagtc tttaattacc cggtcagtct gtacatgcag
     1321 cggcatatcg gcagtttcct gcgctaaggc gtcgatcagt tccataaacc gctgtaaggc
     1381 gctggcagca cgtccggcga gggctttttc ctgcaacagt tcacgacatg cctgccagag
     1441 tgttaactgg cgatcgcgcg atgtctgacg taccacgtcc agcgtccggt caccaatacc
     1501 ccgcgttggc gtattcacca cacgctcaaa ggccgcgtcg tcgttacggt tggcaatcag
     1561 gcgcagatac gagagcgcat ctttgatttc ctggcgttcg aagaagcgca tcccgccgta
     1621 aatacggtac ggcatactcg cctgcaacag tgcttcttcc agcacacgcg actgggcgtt
     1681 actgcggtag agaatggcgc actcggcaag cgccccgccg ttgtcctgcc aggttttgat
     1741 gcggttaacc acaaaacgcg cttcatcgag ttcgttaaaa gcgcaataga gggaaatagg
     1801 ctcaccgtcc gcgccatcgg tccacagttt tttacccaga cgcccgttat tgttttcaat
     1861 cagggcgtta gcggcgctca gaatattgct ggtagagcgg tagttttgct ccagacgaat
     1921 aatttcggca ccggggaaat cattaaggaa acgctgaata ttctccacct gcgccccgcg
     1981 ccagccgtag attgactggt cgtcatcacc gacgatcatc actttgccgg tgtcgcccgc
     2041 cagcaggcgg atccacgcgt actgaatgtt gttggtatcc tggaattcgt ccaccaggat
     2101 attggtaaaa cgttcgcggt agtgttgtag gatatgcggc ttgttaagcc acagttcgtg
     2161 agcgcgcagc agcagctcgg cgaagtccac caggcccgcg cgatcgcacg cttcctgata
     2221 cgcctgatac accttctgcc aggtctgctc caccggatta ccgtagcttt gaatatgatg
     2281 cggacgcagg ccttcatctt tctggctgtt gatgtaccac attgcctggc gcggcggcca
     2341 ctgcttctcg tcgaggttca tggctttgat cagacgctta agcaggcgta gctggtcttc
     2401 gctatcgagg atctggaaat cctgcggcag attggcgtcc atatggtgcg cacgcagcag
     2461 gcggtgcgcc agcccgtgga aggtgcctac ccacatgccg ccctggctgg tgcccatcag
     2521 ttgtccgata cgatgacgca tctccgccgc cgctttgttg gtaaacgtca cagccataat
     2581 cgagtatggc gagcagtttt ccacgctcat cagccaggcg atacgatgca ccagtacgcg
     2641 cgtcttacca ctgcccgcgc ccgccagcac cagaaggttg ctgcgtggcg cggccaccgc
     2701 ttcgcgttgt ttgtcattaa ggctgtcgag caggtaagaa acgtccattg gcaccgccgc
     2761 gtaaaaatag gttgggcgga gaagcgcaac gccctccgcc aaaaagctgg gtatatatac
     2821 agatttgctg attatatcag cgaggtcaga gatgccaacc gggaaatttc cagatgtggc
     2881 agtaaacggc tgtcccaggt ttgcatcaga tcgccatttt ccggtctgat ccaacaagcc
     2941 tgcattccgc tgcgaattgc cccacccacg tcagtggtga gatcgtcccc aacatgtaag
     3001 atctcgccga tcggcacgtt gagtttttcc gcagccaaaa agtacatatc gctgaacggt
     3061 tttgagcgcc cgtgcgggcc agcgcgcagc acaaactcaa aataatcccc caaaccaaac
     3121 agttccggct gggcgttacc gttggtgatc gccaccagcg gccatttctt cgccagctgt
     3181 tttaaggtgt cgtgagtttg ctgcgggacg tcgattcggc tgcgccattt ggcaaagtta
     3241 atcattgctg cgtgtgcgcc tgcactggct tcttcggcac tcagcccggc gtcgagcatc
     3301 gcttgttcaa tcgaacgaaa acgccaacgt gtcacgtcgt gataaatctc gggttccgct
     3361 tcccgtaccg cctggcgcag gcgttgcaga tcttcattct ggaagctgcg cagcgccgga
     3421 tgataatttt gcacaaaggt aagcgcctct cgctcggtgc gcaaaatcac cggacggtta
     3481 tcgtaaaggg tatcatccag gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa
     3541 aaacgcatta tttcccccgt ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt
     3601 gttgaaaatc aagatgagta tagatttgcg tggtggagag gttggcatga cccagtagct
     3661 cctgcacacc acgaagatcg ccgctcgact ccagcatatg cgtagcgaac gagtgacgta
     3721 atttatgtgg gtgaacgtga ttattcagcc cttgttttat gccccattcg gcaaagcgtt
     3781 tctgcacatt acgcgcggag atacgcttgc ccagtttcga cagaaaaagc gcgtcgtctt
     3841 cgctaccaaa caggtcgcgc aaatcaagcc agtgctcaat ccacgccaca gcgttgcgac
     3901 caatcggcag gcggcgctct ttgctgcctt tccccatcac ccacacttcg ccggactcca
     3961 ggtcgaggtg tttgatatcc agccccacca gctcggaaag acgcagaccc gcgccgtaca
     4021 tcacttccag cattgcacgg tcgcgtacag cgaggggatc gttgatatca atatccagca
     4081 gccgattcat atcgtcgacg tcgatatttt tcggcagatg acgcggcgct ttcggtgccg
     4141 aaactccttt cgccgggtta gctttgagtt cgttctggct gaccagccag tcaaaaaagc
     4201 tacgtagcgc agaaagccgt aacgccagac ttgctgctcc cagcccttta cggcgactgc
     4261 gtacagcaaa attgcgtacc atcgctgcat cgcattgttg ccagctttgc aggccgtttt
     4321 cgctggcaaa attgatgatc gcctcaagct gacgctggta gttaagtagg gttatcgggc
     4381 taagctgacg ctccacgctc agataacgta ggtagcgttc tacatcggtg tgtaaatcgg
     4441 tcatacgcgt tcaatccaac gctccagaag ctccggcaac atcagcgcaa tttcatgaag
     4501 taactgcgtt ccctgccctt gttgatagtg actggcatcg cgactggtaa acagcacaac
     4561 acccaaatca gcatcgcttc ccagcatcga catcgccacc gatcccaccg ctttcgcttc
     4621 cggtagcacc accagcagct ccggtccgtt aagcggccca agatagtgct gttcctgccc
     4681 caaacgctga atacgcagcg gttcgaaaga ctgacggctt aatgccagat gagtgtggtt
     4741 cgacggcgca cctaagcgcc agcgatctgg aaacaggcgc agactcgcac cagctaggcc
     4801 gagatcgcgc gcccagcggt gaaagcgcat cagcatatcg tcgagactgc tggcggcggt
     4861 gagactgcgc tgcaggtaga gtaggcgata aaacagccct tcgttggcga tagcctgctc
     4921 catcaacaac gccatgttct cttccagaac atgaatgtga ttacgtgcgc gggccatgtg
     4981 ccactcgacc aacgaaacgg tgccgcgtac cggatgcggc acacgtatcg cttctactgc
     5041 gcgcgcattg cggataaaaa acccaggatt tttaatcaga taatcgacaa ccgcccggtc
     5101 atcaagctcc gtgagtgttt cctgcagttc ttcccctggt tgcttcatag atgaataaat
     5161 ccatcgtaga catgtaccgc cgggccagtc atatataacg ggtgacccgg acctttccag
     5221 gcgatatcaa gacgaccgcc ggggagttcc acgcgtactt cttcgtccag caaaccttgc
     5281 tgaatcccta ccgccaccgc cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc
     5341 ccacgctcat aaacgcgtaa acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata
     5401 ttggcgcgct ccggaaaacg ctcgtgactt tccagaacag gaccaagcgt ttctaccgcc
     5461 gcggtatcga catcatcgac ctgaatcacg caatgcggat ttcccatcga caccacgccg
     5521 cataagattg tctgctcggc ggcgcgcata atataggtct tttccgcttt gttagcgcga
     5581 aacggcacgg cggaaggttc gaagttgggt tcgcccatat ttacgcggac cagatcatca
     5641 tcggtgacgg tcagaaccat ccgcccgttg gcggtgctga cgcggatatc acgcttattg
     5701 gtcagtcctt tcagacgcac aaaacgggca aagcagcgcg caccgttacc gcactgcgcc
     5761 acttcactgc catcagcatt gaaaatgcga tagtgaaaat ccagttcagg atcatacggc
     5821 ggctcaacca ccagcagctg gtcaaaccct acccccaggt gccgatccgc caggcgacga
     5881 atcagctccg gtgaaaaaaa gacattctgc gttaccgcgt cgacgaccat aaaatcgttg
     5941 ccaaggccat gcattttcga gaactgcatc atttactcca atcacgcggg tacagaaact
     6001 gacttttagt aattcacctg ggatggacca tcgccagtgg cgcgatcgtt tttatccggc
     6061 accgtggatt gcgtttgcgt ctctaccggt ttggtcggcg gcggtgcgtt tttatctgca
     6121 ggcgggaaat agagcggacc tttcagaccg cagcccgtca ggctgaagag agtaagtaat
     6181 acagtgagtg ccttaaacac gtttttcatt atggattgcc tgtaaagttc atcgctttct
     6241 gctttctatc atcgcaggag aaggcgtaaa agcaagcgtt tagcgactct ttatgaagtc
     6301 tgaaaagtga ggaaatgctg cgagtataac gcttactcgt gaggatttat gttctgctgg
     6361 cagtttcgtg atcaacacct cgcgccagga tggctttatg cactatcgca atgtggtttg
     6421 tttgttgtct tgttcattgt ttttatcgtc ggcatgggga tgtcggcttt ccgcacctga
     6481 acataatatt taccagaaac agggaaaagg cgtggtgtac ctgcgccctt ataaagaaac
     6541 aaatctttct ctacctgacg tgaattacaa gagtttgcgt cggttaccca atttattaat
     6601 agacccgaca acactagacg aatgggataa agaaccgcct ctgactgatt tgacaaccga
     6661 ctacctctat gagggcgcac aggcctggta tcctcattat tcatggcata gcgatggtcg
     6721 caatatcctc tacgcgggtg aagttgtgca aaaccctccc ggcacacctc ccgtcgatgt
     6781 cgcctcgttc aaggcatggg gtgattttgc cgcagataaa cacagtctct attttgaagg
     6841 caagcgcacc gatgacaatg gtggtggaaa tagcttggat atcaaaacgc tgcatcaggt
     6901 ggaattccgg cctccctggg atcctgactt actggggctg atattacgtg acgctaattt
     6961 cttgtatatc aatgggcatc gcctcgctga ccccgagagt ttccgtgtgc tggcgcagaa
     7021 gtcctgggat cagcgaggaa agttttccac cacgtttaac ccctgcattg ccgtaccatt
     7081 tggcccctgg gataccctgg ctcgtacacg gaccaaaatc ctgctcaacg gcgaacagct
     7141 tgatgccgac ccggacacct tttccgtcgt gcgctggatg cccggctcac tcttgacctg
     7201 gcgtgataaa aacgggctgc agcgtaaagt cctcgacaag gaaaatctgg cgtgggatga
     7261 agatttaaca aagcactgtc tggatttttc tctgctggaa aagaaagtgt tctggcgtaa
     7321 aggacctgct tgtaaacagg aagaattacc cggactcgat ccggagcagt ttcaacccat
     7381 cagtgatgct gtcgcccagc atcaggactc gctttatacc atcatcgaaa cagagtctgg
     7441 taaccgtaag ctggagatcg tgaaacttga tgatcccaat cttattatca acaaacgttt
     7501 caacgccggg aaacgccacg gctatttact tacgcgtgcc gaagggtagc cataccattc
     7561 cggtttacac gtgtttgaat ctgacggacc gctgatctta ctggataacc gttctccgga
     7621 tgaacgcgaa gcccatctta atgaccatcc ctttttgcgc agatggtatg cccgcgataa
     7681 ccgctacatt tacagctttg atggcgcgca gctctggcga taccgcaccg ctgatccgaa
     7741 acaagttcgc ttaatctgga aggaacaaca ttcgggatat gtctatggcg taaattacaa
     7801 aacgggatat ctggacggaa aaattactga tgacggcgaa tttattcctg ccccgcgcaa
     7861 tgaggcgaca aaatgaaggg tcaaataatc acagttgcac tcatcctgtt gggacacaca
     7921 tttctgacgc ctgctgtaca ggccataggg ttcgactatt acaatgatca cggtgttatg
     7981 tcttacggta aaggttattg gggggataag aaaatcattg cccagtttcc ggagatgagc
     8041 agggccggtt tgcgccttgt gaaaaatatc tctgggaaaa cgggttatat accttccgat
     8101 gaaataagca tagaaaatga ggaataccac tgggtgacag acggccgcgt tattctctgg
     8161 cgtggcaaaa tcgttagcaa tccaccggga acactcactg tcgatattgc cagctttcag
     8221 gctatgggcc gcttcgcggt cgataaatat agcctctatt ttgacggaca gcgcaccgaa
     8281 agtaatagcg gcgcgtcacg tgttgagctg gcgacactaa aagctatcga aggtaactct
     8341 accacgctgg tggatagtaa aaatctctac ttgtccggtc gacgtcaggg gagcagcagt
     8401 gatgttactg tattagaaaa aagatggtgg ggtattaatc cacgtcttat gagtgtaaac
     8461 agaaattcat attccaatga tctgcttatc cgcagtgggc agaatattta tttaaatggc
     8521 gttcacctta cggcgaatgc agactcattt gagataattc gctggatacc tcattcactg
     8581 ctggtttttc gcgacaataa gggtctgcat cgttatccct ttggtcaatt atcaggcaaa
     8641 gcgatacccg tagatgatga cgtctctttt gaagtagggg aaagtcgcgt tcgctggcgt
     8701 aaacagctca cgcccgaccg tcagtggagc aagtggatag acctaccagg tattgaacct
     8761 gaacaattcg gtaatgactc caacttactg atagtgtttt atgttcagat aatgcccgat
     8821 gaccttgtca tgcagctcca ccgattttga gaacgacagt gacttccgta cc
//
LOCUS       DAZSIO010000154         8816 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZSIO010000154 DAZSIO010000000
VERSION     DAZSIO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.154"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..1723
                     /gene="iucA"
                     /locus_tag="ACHX5T_003313"
     CDS             <1..1723
                     /gene="iucA"
                     /locus_tag="ACHX5T_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR8468377.1"
                     /translation="TLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDEQ
                     KSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLCE
                     TFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFNR
                     REAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDNQ
                     WLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIKF
                     SLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDLN
                     GNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITVQ
                     QAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSAF
                     MPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLMA
                     RVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFAN
                     PLQAQEV"
     gene            1724..2671
                     /gene="iucB"
                     /locus_tag="ACHX5T_003314"
     CDS             1724..2671
                     /gene="iucB"
                     /locus_tag="ACHX5T_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR8468378.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            2671..4413
                     /gene="iucC"
                     /locus_tag="ACHX5T_003315"
     CDS             2671..4413
                     /gene="iucC"
                     /locus_tag="ACHX5T_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR8468379.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            4410..5747
                     /gene="iucD"
                     /locus_tag="ACHX5T_003316"
     CDS             4410..5747
                     /gene="iucD"
                     /locus_tag="ACHX5T_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR8468380.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            5753..7948
                     /gene="iutA"
                     /locus_tag="ACHX5T_003317"
     CDS             5753..7948
                     /gene="iutA"
                     /locus_tag="ACHX5T_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR8468381.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(8499..8642)
                     /locus_tag="ACHX5T_003318"
     CDS             complement(8499..8642)
                     /locus_tag="ACHX5T_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468382.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
BASE COUNT         2114 a         2243 c         2341 g         2118 t
ORIGIN      
        1 gaccctgccc tctgaaaaac cagccacaga tgtggctgcg cagtgcttcc tgaatgcact
       61 gattcgtgaa accacagact ggaaactgac agaatacccg ccagacgaat tgctcatccc
      121 gctggatgag cagaaatcgc tccatttcag agtggcttat ttctccccaa cccagcatca
      181 ccgctttgca tttcctgcac gtctggtcac ggcatcaggc agttatcctg tcgactttac
      241 cactctctcc cggcttatta ttgataagct acgccatcaa ctgtttctgc ccgttcccct
      301 ctgcgaaact ttccaccagc gcgtgctgga aagccatgtc catacgcaac aggcaattga
      361 tgcccgtcat gactgggccg ctctgcgtga aaaagcgttg aattttggcg aggctgagca
      421 ggcgctgctg acaggacacg ctttccaccc tgcgcctaag tctcatgaac cgtttaaccg
      481 gcgggaggct gaacgctacc tgcctgatat ggcaccccac ttcccactgc ggtggttttc
      541 ggtggataaa acgcaaatcg ccggtgaaag tttgcatctc aaccttcaac agcggctgac
      601 gcgatttgcc gcagagaatg cgcctcagtt actcaacgaa ttaagtgaca accaatggct
      661 gttcccgttg cacccgtggc agggggaata tcttttgcag caggggtggt gccaggcact
      721 tgttgctaaa gggctgatta aagacttagg tgaggccggc acgtcgtggc tgccgaccac
      781 ctcttcccgt tccctctact gtgccaccag ccgcgatatg atcaagttct ccctgagcgt
      841 acggctgacc aactccatcc gtactctgtc cgtgaaagaa gtgaagcgtg ggatgcgcct
      901 ggcacgcctg gctcaaaccg acggctggca gatgctacag gtccgcttcc cgactttccg
      961 ggtaatgcag gaggatggct gggccgggct gctcgatctt aacggcaaca tcatgcagga
     1021 aagtctgttt gccctgcgtg aaaatctgct ggtggatcag ccgaaaagcc agactaacgt
     1081 actggtctcc ctgactcagg ccgcacctga tggcggtgat tcgctgctgg tttcggcggt
     1141 aaaacgcctg agcgatcgcc tcggtatcac tgtgcaacag gccgcccatg catgggttga
     1201 tgcatactgt cagcaagtgc taaagccgct gtttacggct gaagcggatt acggcctggt
     1261 gctgctggcg catcagcaaa atattcttgt ccagatgctt ggggatctgc cggtcggatt
     1321 tatttaccgt gactgtcagg gcagcgcttt tatgcctcac gcgacagact ggctcgattc
     1381 tattggcgag gcgcaggcgg aaaatatttt cacccatgag cagttgctgc gctatttccc
     1441 ttattacctg ctggttaact ccacttttgc tgtgaccgct gcgctggggg ctgccgggct
     1501 ggacagcgaa tcgaatctga tggctcgtgt acgagcatcg ctggctgaag tgcgtgatca
     1561 ggtgactcat aaaacctgtc tcaactacgt gctggaaagt ccgtactgga acgtaaaagg
     1621 taactttttc tgttatctga acgatcataa cgagaacacc attgttgacc cttcggtgat
     1681 ctatttcgat ttcgctaacc cgctgcaggc tcaggaggtc tgaatgtctg aggcaaacat
     1741 tattcacagc agatatggac tgcgctgtga aaaactcgac aagcccctga atcttggctg
     1801 gggactggac aatagcgcgg tgttgcactg tcccggggag ctgccgacag ggtggctgtg
     1861 cgatgcgctg gatcagatat ttatcgccgc accacaactg tcagcagtgg ctctcccctg
     1921 ggccgaatgg cgtgaggagc cacaggcgct gacgcttttt gggcaggtaa aaagcgacat
     1981 tatccatcgt actgccttct ggcagttacc gttatggttg agttcaccgg caaaccgggc
     2041 ctccggtgaa atggtttttg atgcagagcg tgagatttat ttcccgcagc gcccaccccg
     2101 tccacagggt gaggtttacc gccgttacga tccacgtatt cgcaggatgc tgagtttccg
     2161 cattgccgat cccgtttctg atgcagaacg tttcacccgc tggatgaatg atccgcgcgt
     2221 tgagtatttc tgggagcaaa gtggctcact ggaggtacag accgcctatc tggagcgcca
     2281 gctcaccggt aaacatgcat tcccgctgat tgggtgcttc gacgatcggc cattcagcta
     2341 tttcgaaatc tactgggcgg cggaagaccg cattggtcgt cactattcgt ggcagccctt
     2401 tgaccgtggc ctgcatctgc tggttggtga acagcaatgg cgcggggccc actatgtgca
     2461 aagctggctg cgcgggctga cacattacct gctgctggat gagccccgta cgcagcgcac
     2521 agtactggag ccacgcaccg ataaccagcg cctgttccgc catcttgagc ctgcgggata
     2581 ccggacaatt aaagagttcg acttcccaca taagcgctcg cgcatggtga tggcggatcg
     2641 tcatcacttc ttcacggagg tcggtctgta atgaatcaca aggactggga ttttgttaac
     2701 cgtcggctgg tggcaaaaat gttgtctgag atggagtatg agcaggtttt ccacgcggaa
     2761 tctcagggcg atgaccacta ctgcattaac ctgccgggag cacaatggcg cttcatcgct
     2821 gaacgtggta tctggggctg gctgtggatt gatgcccaaa cgttgcgctg cacggacgaa
     2881 ccagtactgg cgcagacgct gctgatgcag ctaaagccgg tgctgtcaat gagtgatgcc
     2941 actgttgcag agcatatgca ggatttgtat gccacgctac tgggcgacct gcaactgctg
     3001 aaggcccgtc gcggactgag tgccagtgac ctgattaatc ttgatgccga tcgtctgcaa
     3061 tgcctgctga gcggtcatcc taaattcgtt tttaataaag gtcgccgtgg ctggggcaaa
     3121 gaggcgctgg aacgatatgc gccagagtat accaacacct tcagactgca ctggctggcg
     3181 gtaaaacgtg aacatatgat ctggcgctgc gataacgatc tggatattca gcagttgttg
     3241 actgccgcaa tggatccgca ggagtttaca cgcttcagtc aggtctggca ggaaaacggg
     3301 ctggatcaca actggctgcc gctgccggta catccgtggc agtggcagca aaaaatcgcc
     3361 acagacttca tcgctgattt tgccgaaggc aggatggtgt ctctcggcga gtttggcgac
     3421 cagtggctcg cccagcagtc gctgcgtacc ctgaccaacg ccagccgccg gggagggctg
     3481 gatatcaagc tgccgctgac catctacaat acctcatgct accgggggat ccctggcaga
     3541 tatatcgctg ccggaccact ggcttcacgc tggctgcaac aggtttttgc gaccgacgcc
     3601 accctggtgc aaagcggcgc agtgatcctt ggtgaaccgg ctgcaggcta tgtgtcccat
     3661 gaaggctatg ccgcgcttgc ccgggctccc tatcgctacc aggaaatgct tggtgttatc
     3721 tggcgggaga atccgtgccg ctggctgaaa ccggatgaaa gtcctgttct gatggcaaca
     3781 ctgatggagt gcgacgaaaa caatcagccg ctggcaggcg catatatcga ccgctccgga
     3841 ctggacgctg aaacctggct tacgcaattg ttccgggtgg ttgtggttcc gctgtatcac
     3901 ctgctttgcc gctacggtgt cgcgcttatt gcacacggac aaaatataac gctcgccatg
     3961 aaagaggggg taccacagcg tgttctgctg aaagacttcc agggcgatat gcggctggtg
     4021 aaagaagcgt ttcccgaaat ggactctttg cctcaggagg tgcgtgatgt cacatcccgt
     4081 ctgagtgcgg actacttaat ccatgatttg cagacgggtc acttcgtgac agtactgcgt
     4141 tttatttcgc cactgatggt tcgtcttggc gtacctgaaa ggcgatttta tcaactgctg
     4201 gcagcagtgt tgagtgatta catgaacaaa catccacaaa tggcagagcg ttttgcgctt
     4261 ttctcactct tcaggccaca aatcattcgc gtggtgctga acccggtaaa actgacctgg
     4321 ccggacctgg atggcggcag tcgcatgctg ccgaattacc ttgagaatct gcaaaatccg
     4381 ctgtggctgg taactcagga atatgaatca tgaaaaaaag tgtcgatttt attggtgtcg
     4441 ggacagggcc atttaatctc agcattgctg cattgtcaca tcagatcgaa gaactgaact
     4501 gtctcttctt tgacgaacat cctcattttt cctggcatcc gggtatgctg gtaccggatt
     4561 gtcatatgca gaccgtcttt ctgaaagatc tggtcagtgc agttgcacct acaaatccct
     4621 acagctttgt taactatctg gtgaagcaca aaaagttcta tcgcttcctt acaagcaggc
     4681 tacgtacagt atcccgtgaa gagttttctg actatctccg ctgggctgct gaagatatga
     4741 ataacctgta tttcagtcat accgttgaaa atattgattt cgacaaaaaa agtcgattgt
     4801 ttctggttca gaccagtcgg ggagaatatt ttgcccacaa tatctgcctt ggtacaggaa
     4861 aacaacctta tttaccaccc tgtgtgaagc atgtgacaca atcctgtttc catgccagtg
     4921 aaatgaatct tcgtcggccg gacctgagtg gaaaacggat aaccgtggtt ggtggaggac
     4981 agagtggtgc agacctgttc cttaatgcat tacgcgggga atggggagaa gcggcggaaa
     5041 taaactgggt ctcacggcgt aataatttta acgcactgga tgaggctgct tttgctgatg
     5101 agtattttac acctgaatat atttcaggct tctccggact gaaggaagat attcgccatc
     5161 agttactgga tgagcagaaa atgacatcgg atggcatcac tgccgattct ttactgacca
     5221 tttatcgtga gttgtaccac cgttttgaag ttctgagaaa accaagaaat atccgtctgc
     5281 tacccagtcg ctcggtaaca actctggaaa gtagtggtcc gggctggagg ttattgatgg
     5341 aacatcatct ggatcagggc agagagagcc tggaaagtga tgtggtgatt ttcgccacag
     5401 gttaccgttc tgcattgcca caaatacttc cctcactgat gcccttgatc accatgcacg
     5461 ataagaacac ctttaaagtg cgtgatgact tcactctgga atggagtggc ccgaaagaaa
     5521 ataacatctt tgcggtcaac gccagcatgc aaactcatgg catcgccgaa ccccagctca
     5581 gcctgatggc ctggcgatct gcacgtattc ttaatcgcgt actggggcgt gatttattcg
     5641 atctcagtat gccgcccgca ctgattcagt ggcgcagcgg cagccggaaa aaaccacagc
     5701 cggaggctgc ttctttaact cactatacag caaatattca ggaataacga tgatgcgcaa
     5761 aaagtatatg ccccgggctc ttggtccgct gcttcttgtc gtgctgtcac cagctgtcgc
     5821 ccagcaaaac gatgataatg agatcatagt gtctgccagc cgcagcaatc gaactgtagc
     5881 ggagatggcg caaaccacct gggttatcga aaatgccgaa ctggagcagc agattcaggg
     5941 cggtaaagag ctgaaagacg cactggctca gttaatcccc ggccttgatg tcagcagcca
     6001 gagtcgaacc aactacggta tgaacatgcg tggccgcccg ctggttgtcc tgattgacgg
     6061 tgtgcgcctc aactcttcac gttccgacag ccgacaactg gactctgtcg atccttttaa
     6121 tatcgaccat attgaagtga tctccggcgc gacggccctg tacggtggcg ggagtaccgg
     6181 agggttgatc aacatcgtga ccaaaaaagg tcagccggaa accatgatgg agtttgaggc
     6241 tggcacaaaa agtggcttta acagcagtaa agatcacgat gagcgcattg ccggtgctgt
     6301 ctccggcgga aatgaccata tctccggacg tctttccgtg gcatatcaga aatttggcgg
     6361 ctggtttgac ggtaacggcg atgccaccct gcttgataac acccagaccg gcctgcagca
     6421 ctccaatcgg ctggacatca tgggaaccgg tacgctgaac atcgatgaat cccggcagct
     6481 tcaactgata acgcagtact ataaaagtca gggagacgac aattacgggc ttaatctcgg
     6541 gaaaggcttt tccgccatca gcgggagcag cacaccatac gtcagtaagg ggctgaattc
     6601 tgaccgcatt cccggcactg agcggcattt gatcagcctg cagtactctg acagtgattt
     6661 cctgggacag gaactggtcg gtcaggttta ctaccgcgat gagtcgttgc ggtactaccc
     6721 gttcccgacg gtaaatgcga ataaacaggc gacggctttc tcctcgtcac agcaggatac
     6781 cgaccagtac ggcatgaaac tgactctgaa cagccaactg atggacggct ggcaaatcac
     6841 ctgggggctg gatgctgagc atgagcgctt tacctccaac cagatgttct tcgatctggc
     6901 tcaggcaagt gcttccggag ggctgaacaa ccataagatt tacaccaccg ggcgctatcc
     6961 gtcatatgac atcaccaatc tggcggcctt cctgcaatcc agctatgaca ttaatgatat
     7021 ttttaccgtt agcggtggcg tacgctatca gtatactgag aacagggtag atgatttcat
     7081 cgactacacg cagcaacaga agattgctac cgggaaggcg atatctgccg acgccattcc
     7141 tggtggttcg gtagattacg ataactttct gttcaatgct ggtctgctga tgcacatcac
     7201 cgaacgtcag caggcatggt tcaatttttc ccagggggtg gcattgccgg atccggggaa
     7261 atattatggt cgcggcatct atggtgcagc agtgaacggc catcttcccc tgacaaagag
     7321 cgtgaacgtc agcgacagta agctggaagg cgtgaaagtc gattcttatg aactgggctg
     7381 gcgctttatc ggtgacaacc tgcggactca aatcgcggca tattactcgc tttccaataa
     7441 gagcgtggaa aggaataaag atctgaccat cagtgtgaag gacgacaggc gccgtattta
     7501 cggcgtggaa ggtgcggtgg actacctgat cccggatact gactggagta ccggtgtgaa
     7561 cttcaatgtg ctgaaaaccg agtcgaaagt gaacggtcaa tggcaaaaat atgacgtgaa
     7621 ggaatcaagt ccatcgaaag cgacagctta cattaactgg gcgccggaac cgtggagtct
     7681 gcgtgtacag agcaccactt ctttcgacgt aagcgatgca gagggtaacg atattaatgg
     7741 ttacactacc gtcgatttta tcagtagttg gcagcttccg gtgggaacac tcagcttcag
     7801 cgttgagaac ctcttcgacc gtgactatac cactgtctgg ggacagcgtg cacctctgta
     7861 ctacagcccg ggttacggcc ctgcttcact gtacgactac aaaggccggg gccgaacctt
     7921 tggtctgaac tactcagtgc tgttctgacg gggatgcttt tgcaacaagg gtatgctgta
     7981 aacaacatgg tccttggtgg ccatgttgtt tacagccagt ataatataca gccttaacaa
     8041 gatattccgt ctccggaatg ggctgttcac cactgaataa cattatgtct gttttatgga
     8101 tctttgactg aaatttatcg catatcctca tttcattgtc cgtctgaaaa ctaaccttac
     8161 aagtaagcgt cgtctcagca ccgtctggca gatcctgaaa ttcctgagag gatgatctgc
     8221 gcccaatatc ctgagccgga actttgctgc aatgaaggcc aacgaaaaat gggggagtat
     8281 gttatagagt acttgctaca gggtaaaaag aggtacctgt cgctggttct ccgttcagtt
     8341 taaccgggag attttctcct gcgcctgtca taaagtccgg taatggagat tgttaatacg
     8401 atggtgcacg atgcatcgtc agtgcccggc ccggaggatg ccccgtagct acatacggat
     8461 cagggttggc aatatcgaat ggcaggctat aggcaaagtt aaagccccat ggagtagcac
     8521 aaaatatgcc gcacaaagga aacggtctga ataacgcagt gatggagaac ttcttcggca
     8581 cactgcaaaa tcgcatcgtg gtcgatcgct aattcgtcgt gaatcactga acgatgttcc
     8641 atgcgaacag atattcgatc atatcgaaat attaaacatt acccctcccc acattgggca
     8701 aaaaaaaaca aaaaacgatc aattagtatt gattcacata atctctcatc attaatgaga
     8761 acattgtcac atatttacaa tatagaactg ttacttttag caagctgtta gtaacc
//
LOCUS       DAZSIO010000155         8753 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZSIO010000155 DAZSIO010000000
VERSION     DAZSIO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.157"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(330..593)
                     /gene="rpsT"
                     /locus_tag="ACHX5T_003319"
     CDS             complement(330..593)
                     /gene="rpsT"
                     /locus_tag="ACHX5T_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR8468383.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            696..914
                     /locus_tag="ACHX5T_003320"
     CDS             696..914
                     /locus_tag="ACHX5T_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR8468384.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            922..1863
                     /gene="ribF"
                     /locus_tag="ACHX5T_003321"
     CDS             922..1863
                     /gene="ribF"
                     /locus_tag="ACHX5T_003321"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR8468385.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1906..4722
                     /gene="ileS"
                     /locus_tag="ACHX5T_003322"
     CDS             1906..4722
                     /gene="ileS"
                     /locus_tag="ACHX5T_003322"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR8468386.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4722..5216
                     /gene="lspA"
                     /locus_tag="ACHX5T_003323"
     CDS             4722..5216
                     /gene="lspA"
                     /locus_tag="ACHX5T_003323"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR8468387.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5303..5752
                     /gene="fkpB"
                     /locus_tag="ACHX5T_003324"
     CDS             5303..5752
                     /gene="fkpB"
                     /locus_tag="ACHX5T_003324"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR8468388.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5754..6704
                     /gene="ispH"
                     /locus_tag="ACHX5T_003325"
                     /gene_synonym="lytB"
     CDS             5754..6704
                     /gene="ispH"
                     /locus_tag="ACHX5T_003325"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR8468389.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6770..7684
                     /gene="rihC"
                     /locus_tag="ACHX5T_003326"
     CDS             6770..7684
                     /gene="rihC"
                     /locus_tag="ACHX5T_003326"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR8468390.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7851..8672
                     /gene="dapB"
                     /locus_tag="ACHX5T_003327"
     CDS             7851..8672
                     /gene="dapB"
                     /locus_tag="ACHX5T_003327"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR8468391.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
BASE COUNT         2012 a         2167 c         2469 g         2105 t
ORIGIN      
        1 aggtctaatg aggagatatt tatgaatggt taaagtatat aggaatttaa ttcaaaaata
       61 gagtggtctt aagtttaata cctcaatgtg tatcacaata tccatattct ttgtggggga
      121 gtctggagat tgagtagata ttcttgttca gaatgtatca gccgatggtt ctacgattct
      181 taagccacga agagttcaga tagtacaacg gcatgtctct tttgactatc tggcaaccgg
      241 cagtgtgttc tctcacgcat cacaaaagca gcaggcataa aaaaacccgc ttgcgcgggc
      301 tttttcacaa agcttcagca aattggcgat taagccagtt tgttgatctg tgcagtcagg
      361 ttagccttat gacgtgcagc tttgtttttg tggatcagac ctttagcagc ctgacggtcc
      421 acgatcggtt gcatttcgtt aaatgctttc tgtgcagcag ctttgtcgcc agcttcgata
      481 gctgcgtata ctttcttgat gaaagtacgc atcatagagc gacggcttgc gttgtgctta
      541 cgagcctttt cagactgaat ggcgcgcttc ttagctgatt tgatattagc caaggtccaa
      601 ctcccaaatg tgttctatat ggacaattca aaggccgagg aatatgccct tttagccttc
      661 ttttgtcaat ggatttgtgc aaataagcgc cgttaatgtg ccggcactcg ttacgtagtg
      721 atggcgcagg attctaccag cttgcggggt gtgaatacag cttttccgcg ataaaaattg
      781 cagcaggcgg tcagtttctt cccgtgattt gctccatggc aatgaaaagc cacttctttc
      841 tgatttcggt actcaatcgc cggttaacct tgaccgctgt acaaggtata ctcggacgat
      901 tttcactgtt ttgagccaga catgaagctg atacgcggca tacataatct cagccaggcc
      961 ccgcaagaag ggtgtgtgct gactattggt aatttcgacg gcgtgcatcg cggtcatcgc
     1021 gcgctgttac agggcttgca ggaagaaggg cgcaagcgca acttaccggt gatggtgatg
     1081 ctttttgaac ctcaaccact ggaactgttt gctaccgata aagccccggc aagactgacc
     1141 cggctgcggg aaaaactgcg ttaccttgca gagtgtggcg ttgattacgt gctgtgcgtg
     1201 cgtttcgaca ggcgttttgc ggcgttaacc gcgcaaaatt tcatcagcga tcttttggtg
     1261 aagcatttgc gcgtaaaatt tcttgccgta ggtgatgatt tccgctttgg cgctggtcgt
     1321 gaaggcgatt tcttgttatt acagaaagct ggcatggaat acggcttcga tatcaccagt
     1381 acgcaaactt tttgcgaagg tggtgtgcgt atcagcagca ccgccgtgcg tcaggcgctg
     1441 gctgatgaca atctggctct ggcagaaagt ttactggggc acccgtttgc catctccggg
     1501 cgtgtagtcc acggtgatga attagggcgc actataggtt tcccgacggc gaatgtaccg
     1561 ctacgccgtc aggtttcccc ggtgaaaggg gtttatgcgg tagaagtgct gggcctcggt
     1621 gaaaagccgt tacccggcgt ggcaaacatc ggaacacgcc caacggttgc cggtattcgc
     1681 cagcagctgg aagtgcattt gttagatgtt gcaatggacc tttacggtcg ccatatacaa
     1741 gtagtgctgc gtaaaaaaat acgcaatgag cagcgatttg cgtcgctgga cgaactgaaa
     1801 gcgcagattg cgcgtgatga attaaccgcc cgcgaatttt ttgggctaac aaaaccggct
     1861 taagcctgtt atgtaatcaa accgaaacac ggaaccgaga atctgatgag tgactataaa
     1921 tcaaccctga atttgccgga aacagggttc ccgatgcgtg gcgatctcgc caagcgcgaa
     1981 ccgggaatgc tggcgcgttg gactgatgat gatctgtacg gcatcatccg tgcggctaaa
     2041 aaaggcaaaa aaaccttcat tctgcatgat ggccctcctt atgcgaatgg cagcattcat
     2101 attggtcact cggttaacaa gattctgaaa gacattatcg tgaagtccaa agggctttcc
     2161 ggttatgact cgccgtatgt gcctggctgg gactgccacg gtctgccgat cgagctgaaa
     2221 gtagagcaag aatacggtaa gccgggtgag aaattcaccg ccgccgagtt ccgcgccaag
     2281 tgccgcgaat acgcggcgac ccaggttgac ggtcaacgca aagactttat ccgtctgggc
     2341 gtgctgggcg actggtcgca cccgtatctg actatggact tcaaaactga agccaacatc
     2401 atccgcgcgc tgggcaaaat catcggcaat ggtcacctgc acaaaggcgc gaagccggtg
     2461 cactggtgcg tagactgccg ttctgcactg gcagaagcgg aagttgagta ttacgacaaa
     2521 acttctccgt ccattgacgt cgctttccag gcggtcgatc aggatgcgct gaaagcgaaa
     2581 tttggcgtaa gcaacgttaa cggcccaatc tcgctggtga tctggaccac tacgccgtgg
     2641 actctgcctg cgaaccgcgc aatctctatt gcacctgatt tcgactatgc gctggtgcag
     2701 atcgacggtc aggccgtgat tctggcgaaa gatctggttg aaagcgtaat gcagcgtatc
     2761 ggcgtgaccg attacaccat tctcggcacg gtaaaaggtg cagagcttga gctgctgcgc
     2821 tttacccatc cgtttatggg cttcgacgtt ccggcaatcc tcggcgatca cgttaccctg
     2881 gatgccggta ccggtgccgt tcacaccgcg cctggccacg gcccggacga ctatgtgatc
     2941 ggtcagaaat acggcctgga aaccgctaac ccggttggcc cggacggcac ttatctgccg
     3001 ggcacttatc cgacgctgga tggcgtgaac gtcttcaaag cgaacgacat cgtcgttgcg
     3061 ctgctgcagg aaaaaggcgc gctgctgcac gttgagaaaa tgcagcacag ctatccgtgc
     3121 tgctggcgtc acaaaacgcc gatcatcttc cgcgcgacac cgcagtggtt cgtcagcatg
     3181 gatcagaaag gtctgcgtgc gcagtcactg aaagagatca aaggcgtgca gtggctcccg
     3241 gactggggcc aggcgcgtat cgagtcgatg gttgctaacc gtcctgactg gtgtatctcc
     3301 cgtcagcgca cctggggcgt accgatgtca ctgttcgtgc acaaagacac ggaagagctg
     3361 catccgcgta ccctcgaact gatggaagaa gtggctaaac gcgttgaagt tgacggcatc
     3421 caggcgtggt gggatctcga tgcgaaagag atcctcggcg acgaagctga ccagtatgtg
     3481 aaagtgccgg atactctcga cgtatggttt gactccggat ctacccactc ttctgttgtt
     3541 gacgtgcgtc cggaatttgc cggtcacgca gcggacatgt atctggaagg ttctgaccag
     3601 caccgtggtt ggttcatgtc ttccctaatg atctccaccg cgatgaaagg caaagcgccg
     3661 tatcgtcagg tactgaccca cggctttacc gtggatggtc agggtcgcaa gatgtctaaa
     3721 tccatcggca acaccgtttc gccgcaggat gtgatgaaca aactgggcgc ggatattctg
     3781 cgtctgtggg tggcatcaac cgactacacc ggtgaaatgg ccgtttctga cgagatcctg
     3841 aaacgtgctg ccgatagcta tcgtcgtatc cgtaacaccg cgcgcttcct gctggcaaac
     3901 ctgaatggtt ttgatccagc aaaagatatg gtgaaaccgg aagagatggt ggtactggat
     3961 cgctgggccg taggttgtgc gaaagcggca caggaagaca tcctcaaggc gtacgaagca
     4021 tacgatttcc acgaagtggt acagcgtctg atgcgcttct gctccgttga gatgggttcc
     4081 ttctacctcg acatcatcaa agaccgtcag tacaccgcca aagcggatag cgtggcgcgt
     4141 cgtagctgcc agactgcgct gtatcacatc gctgaagcgc tggtgcgctg gatggcacca
     4201 atcctctcct tcaccgctga tgaagtgtgg ggctatctgc cgggcgaacg tgaaaaatac
     4261 gtcttcaccg gtgagtggta cgaaggcctg tttggtctgg cagacagtga agcaatgaac
     4321 gatgcgttct gggacgagct gttgaaagtg cgtggcgaag tgaacaaagt cattgagcaa
     4381 gcgcgtgccg acaagaaagt gggtggctcg ctggaagcgg cagtaacctt gtatgcagaa
     4441 ccggaactgg cggcgaaact gaccgcgctg ggcgatgaat tacgatttgt cctgttgacc
     4501 tccggcgcta ccgttgcaga ctataacgac gcacctgctg atgctcagca gagcgaagta
     4561 ctcaaagggc tgaaagtcgc gttgagtaaa gccgaaggtg agaagtgccc acgctgctgg
     4621 cactacaccc aggatgtcgg caaggtggcg gaacacgcag aaatctgcgg ccgctgtgtc
     4681 agcaacgtcg ccggtgacgg tgaaaaacgt aagtttgcct gatgagtcaa tcgatctgtt
     4741 caacagggct acgctggctg tggctggtgg tagtcgtgct gattatcgat ctgggcagca
     4801 aatacctgat cctccagaac tttgctctgg gggatacggt cccgctgttc ccgtcgctta
     4861 atctgcatta tgcgcgtaac tatggcgcgg cgtttagttt ccttgccgat agcggcggct
     4921 ggcagcgttg gttctttgcc ggtattgcga ttggtattag cgtgctcctg gcagtgatga
     4981 tgtatcgctc gaaggccacg cagaagctaa acaatatcgc ttacgcgctg attattggcg
     5041 gcgcgctggg caacctgttc gaccgcctgt ggcacggctt cgttgtcgat atgatcgact
     5101 tctacgtcgg cgactggcac ttcgccacct tcaaccttgc cgatactgcc atttgtgtcg
     5161 gtgcggcact gatcgtgctg gaagggtttt taccttccaa agcgaaaaaa caataataaa
     5221 tcctgccgga tgcggcctac agattgctac gaaatcgtag gccgcatccg gcaaaaatcc
     5281 tcaaaaataa gagcaaacct gcatgtctga atctgtacag agcaatagcg ccgtcctggt
     5341 gcacttcacg ctaaaactcg acgatggcac caccgccgag tctactcgca acaacggtaa
     5401 accggcgctg ttccgcctgg gcgatgcttc tctttctgaa gggctggagc aacacctgct
     5461 ggggctgaaa gtgggcgaca aaaccacctt ctcgctggag ccagatgcgg cgtttggcgt
     5521 gccgtcaccg gacctgattc agtacttctc ccgccgtgaa tttatggatg caggcgagcc
     5581 agaaattggc gcaatcatgc tttttaccgc aatggatggc agtgagatgc ctggtgtgat
     5641 ccgcgaaatt aacggcgact ccattaccgt tgatttcaac catccgctgg ccgggcagac
     5701 cgttcatttt gatattgaag tgctggaaat cgatccggca ctggaggcgt aacatgcaga
     5761 tcctgttggc caacccgcgt ggtttttgtg ccggggtaga ccgcgctatc agcattgttg
     5821 aaaacgcgct ggccatttac ggcgcaccga tatatgtccg tcacgaagtg gtgcataacc
     5881 gctacgtggt cgatagcttg cgcgagcgtg gggctatctt tattgagcag attagcgaag
     5941 tgccggacgg cgcgatcctg attttctccg cacacggtgt ttctcaggcg gttcgtaacg
     6001 aagcgaaaag ccgcgatttg acggtgtttg atgccacctg tccgctggtg accaaagtgc
     6061 atatggaagt tgcccgcgcc agtcgccgtg gcgaagaatc tattctcatc ggccacgccg
     6121 ggcacccgga agtggaaggg acgatggggc agtacagcaa ccccgaaggg ggaatgtatc
     6181 tagtcgaatc gccagacgat gtgtggaaac tgacggtcaa aaacgaagag aagctctcct
     6241 ttatgaccca aaccacgctg tcggtggatg acacgtctga tgtgatcgac gcgctgcgta
     6301 aacgcttccc gaaaattgtc ggtccgcgca aagatgacat ctgctacgcc acgactaacc
     6361 gtcaggaagc ggtacgcgcc ctggcagaac aggcggaagt tgtgttggtg gtcggttcga
     6421 aaaactcctc caactccaac cgtctggcgg agctggccca gcgtatgggc aaacacgcgt
     6481 ttttgattga cgatgcgaaa gatatccagg aagagtgggt gaaagaggtt aaatgcgtcg
     6541 gcgtgactgc gggcgcatcg gctccggata ttctggtgca gaatgtggtg gcacgtttgc
     6601 agcagttggg tggtggtgaa gccattccgc tggaaggccg tgaagaaaat attgttttcg
     6661 aagtgccgaa agagctgcgt gtcgatattc gtgaagtcga ttaagtcatt agcagcctaa
     6721 gttatgcgaa aatgccggtc ttgttaccgg cattttttat gaagaaaaca tgcgtttacc
     6781 tatcttcctc gatactgacc ccggcattga cgatgccgtc gccattgccg ccgcgatttt
     6841 tgcccccgaa ctcgacctgc aactgatgac caccgtcgcg ggtaatgtct cggttgagaa
     6901 aactacccgc aatgccctgc aactgctgca tttctggaat gcggagattc cgctcgccca
     6961 gggagccgct gtgccgctgg tacgcgcacc gcgtgatgca gcatctgtgc acggcgaatc
     7021 gggaatggct ggctacgact ttgttgagca caaccgaaag ccactcggga taccggcgtt
     7081 tctggcgatt cgggatgccc tgatgcgtgc accagagccc gttaccctgg tggccatcgg
     7141 cccgttaacc aatattgcgc tgttactttt acaatgcccg gaatgtaaac cgtatattcg
     7201 tcgtctggtg atcatgggcg gttctgctgg tcgcggcaac tgtacaccaa atgccgagtt
     7261 taatatcgct gccgatccag aagcggctgc ctgtgtcttc cgtagcggca tcgaaattgt
     7321 catgtgcggt ctggatgtca ccaatcaggc aatattaacc cctgactatc tcgctacttt
     7381 accggagttg aatcgcaccg gaaaaatgct ccacgccctg tttagccact accgtagcgg
     7441 cagtatgcaa agcggtctgc gaatgcacga tctctgcgcc atcgcctggc tggtgcgccc
     7501 ggacttattc accctcaagc cgtgttttgt cgcggtagaa actcaaggcg aatttacctc
     7561 aggcacgacg gtggttgata tcgacggttg cctgggcaaa ccagccaatg tacaggtggc
     7621 attggatctg aatgtaaaag gcttccaaca gtgggtggct gaggtgctgg ctctggtgcc
     7681 gtaacctgtc acatgttatc ggcatgcagt cattcatcga ctcatgcctt tcactgatat
     7741 ccctccctgt ttatcattaa tttctaatta tcagcgtttt tggctggcgg cgtagcgatg
     7801 cgctggttac tctgaaaacg gtctatgcaa attaacaaaa gagaatagct atgcatgatg
     7861 caaacatccg cgttgccatc gcgggagccg gggggcgtat gggccgccag ttgattcagg
     7921 cggcactggc attagaaggc gtgcagctgg gcgcggcgct ggagcgcgaa ggatcttctt
     7981 tactgggcag cgacgccggt gagctggctg gtgcagggaa aacaggcgtt accgtgcaaa
     8041 gcagtcttga tgcgataaaa gatgattttg atgtgtttat cgattttacc cgtccggaag
     8101 gtacgctgaa ccatctcgct ttttgtcggc agcatggcaa agggatggtg atcggcacta
     8161 cggggtttga cgaagccggt aaacaggcaa ttcgtgacgc cgccgccgat attgcgattg
     8221 tctttgccgc caattttagc gttggcgtta acgtcatgct taagctgctg gagaaagcag
     8281 ccaaagtgat gggtgactac accgatatcg aaattattga agcacatcat agacataaag
     8341 ttgatgcgcc gtcaggcacc gcactggcaa tgggagaggc gatcgcccac gcccttgata
     8401 aagatctgaa agattgcgcg gtctacagtc gtgaaggcca taccggtgaa cgtgtgccag
     8461 gtaccattgg ttttgctacc gtgcgtgcag gtgacatcgt tggcgaacat accgcgatgt
     8521 ttgccgatat tggcgagcgt ctggagatca cccataaggc gtccagccgc atgacatttg
     8581 ctaacggtgc ggtaagatcg gctttgtggt tgagtggtaa ggaaagtggt ctttttgata
     8641 tgcgagatgt gcttgatctc aataatttgt aaccacaaaa tgtttattat gatgcaaaag
     8701 taacacattt aatttattaa ttataaaggg ctttaatttt tagccctttt att
//
LOCUS       DAZSIO010000156         8739 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZSIO010000156 DAZSIO010000000
VERSION     DAZSIO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8739
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.158"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            135..671
                     /gene="hpt"
                     /locus_tag="ACHX5T_003328"
     CDS             135..671
                     /gene="hpt"
                     /locus_tag="ACHX5T_003328"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR8468392.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            complement(712..1374)
                     /gene="can"
                     /locus_tag="ACHX5T_003329"
     CDS             complement(712..1374)
                     /gene="can"
                     /locus_tag="ACHX5T_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR8468393.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            1483..2409
                     /locus_tag="ACHX5T_003330"
     CDS             1483..2409
                     /locus_tag="ACHX5T_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8468394.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            2406..3176
                     /locus_tag="ACHX5T_003331"
     CDS             2406..3176
                     /locus_tag="ACHX5T_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8468395.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            3281..3721
                     /locus_tag="ACHX5T_003332"
     CDS             3281..3721
                     /locus_tag="ACHX5T_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR8468396.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            3785..5014
                     /locus_tag="ACHX5T_003333"
     CDS             3785..5014
                     /locus_tag="ACHX5T_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR8468397.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5018..5398)
                     /gene="panD"
                     /locus_tag="ACHX5T_003334"
     CDS             complement(5018..5398)
                     /gene="panD"
                     /locus_tag="ACHX5T_003334"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR8468398.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            5672..6574
                     /gene="rpnC"
                     /locus_tag="ACHX5T_003335"
     CDS             5672..6574
                     /gene="rpnC"
                     /locus_tag="ACHX5T_003335"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR8468399.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            complement(6648..7499)
                     /gene="panC"
                     /locus_tag="ACHX5T_003336"
     CDS             complement(6648..7499)
                     /gene="panC"
                     /locus_tag="ACHX5T_003336"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR8468400.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(7511..8305)
                     /gene="panB"
                     /locus_tag="ACHX5T_003337"
     CDS             complement(7511..8305)
                     /gene="panB"
                     /locus_tag="ACHX5T_003337"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR8468401.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            complement(8418..>8739)
                     /locus_tag="ACHX5T_003338"
     CDS             complement(8418..>8739)
                     /locus_tag="ACHX5T_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8468402.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
BASE COUNT         2104 a         2048 c         2296 g         2291 t
ORIGIN      
        1 atcacaattc gagctttatt aacagattcc gcgaatgaat agttttactg gtatactgcg
       61 cgtcttgcgc tttgttgcgg tgccaaaacc tgcccgtgcg aagtgatttg tttttaaatc
      121 atatggttag agatatgaaa catactgtag aagtaatgat ccccgaagcg gagattaaag
      181 cgcgtatcgc cgaactgggt cgtcagatta ctgagcgtta caaagacagc ggcagcgata
      241 tggtgctggt gggtctgctg cgtggctcat ttatgtttat ggcggacctg tgccgtgaag
      301 ttcaggtatc tcatgaagtc gactttatga ccgcctccag ctacggtagc ggcatgtcca
      361 ccacccgtga tgtgaaaatc ctcaaagatc tggatgaaga tatccgtggc aaggacgtgc
      421 tgattgttga agatatcatc gactcgggga atacactgtc gaaagtgcgt gagatcttaa
      481 gcctgcgcga accgaagtcg ttggcgattt gtacgctgct ggataaaccg tcccgtcgtg
      541 aagtgaacgt cccggttgaa tttatcggtt tctcgatccc ggatgagttt gtggtgggtt
      601 acggcattga ttacgcacag cgttaccgtc atctgccgta tattggcaaa gtgattctgc
      661 tggacgagta agtgtgaagt tgccggatgt gttgcatccg gcatggcatt tttatttgtg
      721 gttggcgtgt ttcagcttga ggttggaaat cccgtgacgg taacgttgct caagggtttc
      781 gcggttggtg gcggtaacat ccagatcacg cagcaagccg tcgtgaatgc cgtaggccca
      841 gccgtgaatg gtaactttct gcccgcgttt ccacgctgat tgcataatgg tggagtggcc
      901 caggttatac acctgttcca tgacgttcag ttcacacaag gtatccagac ggcgctcttg
      961 cggcatttcg ccgagcaatg agctatgttt gaaccagata tcgcggatat gcagcagcca
     1021 gttgttgata agccccagtt ccgggttttc aacggcggct tgtacgccgc cgcaaccgta
     1081 gtggccacag ataataatgt gttcaacttc gagtacatcc actgcatact gaaccacgga
     1141 aaggcagttc aggtcggtgt gaatgactag gttagcaaca ttacggtgaa caaagagttc
     1201 gcccggctca agaccggtta aacgttctgc aggaacgcga ctgtcggaac atccaatcca
     1261 tagaaagcgc ggtttttgcg cttgtgccag tttctcaaaa aacccgggat cctcttccac
     1321 cagcattttt gaccatagtg cattgttgct gatgagtgta tctatgtctt tcatggaggt
     1381 taacgacctg taaccaaata attacgtttg gctaatatag ggcaactccg ggacgattta
     1441 aaccacagat aaagtgtaag aacgtaaggt aagtaaaaat ttatgaccat tgcactggaa
     1501 cttcaacagc ttaaaaaaaa ctatccaggc ggcgttcagg cgcttcgtgg aatagatttg
     1561 caggtcgaag cgggtgattt ttatgcgctt ctcgggccga acggggccgg gaaatcgacc
     1621 actatcggta ttatcagctc tctggtaaat aaaacctccg ggcgggtcag cgtatttggt
     1681 tacgatctcg agaaagatgt cgtgaacgct aaacgtcagt tgggactggt gccgcaggaa
     1741 tttaacttca acccgtttga aaccgtgcag caaattgtgg tgaatcaggc agggtactac
     1801 ggtgtggagc gcaaagaggc gtacatccgc agcgaaaaat atcttaagca gctcgattta
     1861 tggggaaaac gcaacgaacg tgcgcgtatg ttatctggcg ggatgaagcg ccgtttaatg
     1921 attgcccgtg cgttaatgca tgaacctaaa ctactgattc tcgacgaacc gaccgcaggc
     1981 gtggatattg aacttcgccg ctcaatgtgg ggctttttga aggatttaaa cgacaaaggc
     2041 accaccatca ttcttaccac acactacctg gaagaagcag aaatgctgtg ccgcaatatc
     2101 ggcattattc aacatggtga gctggtggaa aatacctcga tgaaggcgct gctggcgaag
     2161 ctgaaatcgg aaacctttat tctcgatctc gcaccgaaaa gtccgttacc gaagctcgat
     2221 ggctatcagt atcgactggt cgataccgcg acgctggaag ttgaagtgct acgtgagcag
     2281 gggatcaaca gcgtatttac gcagttaagt gagcagggca ttcaggtatt aagtatgcgt
     2341 aacaaagcta accgtctgga agagctgttt gtttcactgg ttaatgaaaa acaaggagat
     2401 cgcgcatgat gcatctttac tgggtggcgc taaaaagcat ctgggcgaaa gagatccatc
     2461 gctttatgcg tatctgggtg cagacgctgg tgccgccagt catcaccatg accctttact
     2521 ttattatctt cggtaacctg ataggttcgc gtattggcga tatgcatggc ttcagctata
     2581 tgcagttcat cgtaccgggg ctgatcatga tgtcggtgat caccaacgcc tacgctaacg
     2641 tcgcgtcatc attttttggt gccaagttcc agcgtaatat tgaagagctg ctggtagcgc
     2701 cggttccgac tcacgtcatt attgccggat atgtcggcgg tggcgtggcg cgtggtctgt
     2761 ttgttggcat tcttgtgacg gcaatttcac tgttttttgt gccgtttcag gtgcattcgt
     2821 gggtattcgt tgccttaacc ctggtgctca cggcggtgct attctccctt gcgggtttgc
     2881 tgaacggcgt gtttgccaaa acgttcgatg acatcagcct ggtgccaacc tttgtgttaa
     2941 cgccactcac gtatttgggc ggggtctttt actcactgac tttgttgccg ccgttctggc
     3001 aagggctgtc gcacctgaac ccaatcgttt atatgatcag tggtttccgc tacggcttcc
     3061 tcggtataaa tgatgtgccg ctggtcacta cctttggcgt actggtggtc tttattgtgg
     3121 cgttttattt gatctgttgg tcgctgatcc aacgtggacg tggtttgcgt agctaaggct
     3181 atttcctctc ctctggattc gggggagagg agttttgacg gctatcaccc tttatcaaca
     3241 atggtcaggg taggctgatt ttcggctaag gaggaaggcg atgttaggtt gggtaattac
     3301 ctgtcacgat gaccgggcgc aagagatact ggatgcgctg gagaaaaaac atggggcact
     3361 tcttcagtgc cgggccgtga atttctggcg cggattaagc tctaatatgc tcagccgcat
     3421 gatgtgcgat gctctgcatg aagcggactc tggtgagggt gtcatcttct taaccgatat
     3481 agccggagcg ccaccgtatc gcgtggcttc attattaagc cacaaacact cccgttgcga
     3541 agtgatttct ggtgtcacgt taccgttaat tgaacagatg atggcttgcc gtgaaaccat
     3601 gaccagttca gagtttcgtg agtgtattgt cgaactgggt gggccggagg tgagtagtct
     3661 ttggcaccaa caacaaaaaa atccgccttt cgtcctcaaa cataatttgt atgagtatta
     3721 acccgcgatt ctgatggcgc ttttgctaca ataaaagcgt tgtttcaccc tcggttattt
     3781 tttcatgtat aaacaagctg ttattctcct gctgatgctg tttaccgcaa gtgtcagtgc
     3841 cgcgttacct gcccgttata tgcaaaccat cgaaaatgct gcggtctggg cgcaaattag
     3901 tgacaagatg gtgaccgtgg ggaatattcg ggccggacaa atcattgccg tggagcccac
     3961 tgccgcaagt tattacgcat ttaattttgg ctttggtaaa gggtttatcg ataaagggca
     4021 tctcgagcca gttcagggac gacaaaaagt tgaagacggt ttgggtgacc tcaacaagcc
     4081 gctgagtaat cagaacttaa ttacctggaa agatacgccg gtctacaacg cgccgagtgt
     4141 gggcagtgcg ccatttgggg tactggcgga caatttgcgc tacccgattt tgcataaact
     4201 gaaagacagg ttaaatcaaa cctggtatca gatccgtatt ggcgatcgac tggcctatat
     4261 cagcgcgctg gatgcccaac ccgataatgg cctgccggtg ctaacttatc accatattct
     4321 gcgcgacgaa gaaaacaccc gttttcgcca tacttcgacg accacctcgg tacgcgcttt
     4381 caataaccaa atggcctggc tgcgcgacag gggatacgcg acactgagca tggctcagct
     4441 ggaaggttac gtgaagaata cgatcaatct ccctgcgcga gcggtggtga ttacctttga
     4501 tgatggcctc aagtcggtga gccgctatgc gtatcctgtg ttgaaacaat atggcatgaa
     4561 ggcgacggtg tttattgtta cctcacgcat caaacgtcac ccgcagaagt ggaacccaaa
     4621 atcgctgcaa tttatgagcg tttctgagct taacgaaatt cgcgatgttt ttgatttcca
     4681 gtcacatacc cattttttgc atcgggtaga tggttatcgc cgacccatat tactgagccg
     4741 tagtgagcac aatattctgt ttgattttgc acgttcacgc cgcgctctgg cgcaatttaa
     4801 tccacatgtc tggtatcttt cgtatccgtt tggcggcttt aatgacaaag ccgtgaaggc
     4861 agcaaacgat gccggatttc acctggcggt gacaaccatg aaaggcaaag taaaaccggg
     4921 ggataatccg ttgttactaa aacgacttta tatcttaaga acggattcgc tggagaagat
     4981 gtcgcggctg gtgagtaacc agccgcaggg ataacaatca agcaacctgt accggaatcg
     5041 ctttcgcggt acgtttcatt tcattgtcgc cttcaaaata ggcgacattg ggtcgccagg
     5101 tgcgagcttc ttcatctggc atggtaacga agctggcgat gatgacaata tcgccgacgc
     5161 tggcgcagtg ggccgccgca ccgttaacag aaataattct cgaaccgcgt tctgccgcga
     5221 tggcataagt ggagaaacgc ttgccgttgg tgacattcca gatatcgatg gcttcgtttt
     5281 cgagaatacc ggctgcgtca agaaaatcct ggtcaatggc gcaagaacct tcatagtgca
     5341 ggtccgcatg agtcactttc acgcggtgga gtttgccctg cagcatcgtg cgaatcataa
     5401 cttctacctt tctaccctgt cgttaacgaa gcaggcggtg cctgctttga ggaaattctc
     5461 acgcagtatt gcccgatttt tgatgagtgt ctactcatct gatggcattt gcgtcagcag
     5521 tttgcgtacc gcgccgaagc gtgccatgta acgcctgtaa cgaattgata tttttatgaa
     5581 cgctgctcgg taaacaataa agaggtggct gacgatagca cgccttgact ggatactgcc
     5641 ttcacgcaag gccacacgga agacagggaa gatggatgca ccgagtacca caccgcatga
     5701 cgcggtattt aaacaatttt taatgcatgc ggagacggct cgcgactttc tggagataca
     5761 tttgccagtg gaattacgcg aactttgtga tctcaacacg cttcatttag agtcggggag
     5821 tttcattgaa gagtgcctga aaggtcacag cacggacgtg ctctattcca tgcaaatgca
     5881 gggcaatccc ggttatctgc atgttgtgat tgaacaccaa agcaagccgg ataagaaaat
     5941 ggcctttcgc atgatgcgtt attctattgc tgccatgcac cggcatctgg aggctggcca
     6001 cgataagctg ccgctggtgg tgccgatact gttttatcag ggcgaggcca caccttatcc
     6061 gctctcaatg tgctggtttg atatgtttta ctcgccggag ctggcgcgac gcgtctataa
     6121 cagtcctttc ccgctggtgg atatcaccat cacaccggat gacgaaatca tgcaacatcg
     6181 gcggattgcg attctcgaac tactgcaaaa acatattcgc cagcgcgact taatgttatt
     6241 gctggagcaa ctggtcacgc tgattgacga agggtacact agcggaagtc agttagttgc
     6301 catgcaaaac tatatgctgc aacgcggtca tactgaacaa gcggatttgt tttatggtgt
     6361 gctgagagac agggaaacgg gagggaagtc tatgatgacg ctggcgcagt ggtttgaaga
     6421 gaaagggatt gagaagggga ttcagcaggg aagacaggag gaaaggcaag aattcgccct
     6481 gcgtcttcta agtaaaggaa tgtctcggga agacgttgca gagatggcaa atttacctct
     6541 tgctgagatt gataaggtaa ttaaccttat ttaagttacc tgtgttatga cagatgacgt
     6601 ggggtaaatt aataactggc gccatcagcc gtagcgccag ttaagtatta cgccagctcg
     6661 accattttgt tgtcgatcag gcgagcatcg ccaagccagg cggctaccag aattactgcc
     6721 cgtttgctgg tttcagaaac ttccagcaat gtgtcggcat cgcgaatctg aatatcatcg
     6781 gcgcggaagc ctttttcatt cagttcttgc cccgcaatgg taataatttc atcgagatcc
     6841 cgttccccag cctgcaattt gtcagcaatc gaacttaaaa ctttgtacag accaggcgca
     6901 attttgcgtt gttccgccgt cagataaccg ttacgggaac ttagcgccag accgtctttg
     6961 gcgcgcataa ttggcacacc gacaatctca atatcgaagc ccatatcggc aaccattttg
     7021 cggatcaacg ccagttgctg aaaatctttt tcaccgaagc aggcgatgtc cggctggacc
     7081 aggttgaaca gcttgctgac aatagtcgaa acgccgcgaa aatgtcccgg acggctggca
     7141 ccttccagca tggtcgaaag gccaggaacg tcaacgtaag tgtgggtttc agtaccgttt
     7201 gggtagatct cttttaccga aggggcgaaa actaaatcca ctttacgttt gtttagcttc
     7261 tcgcagtcct cctgcaaggt ccgtggataa cgtgccagat cttccgggcg gtcgaactgc
     7321 atcgggttaa cgaaaatact gacgacgacc acatcggcgc gggctttggc ttcgtcgacc
     7381 agcttcatat ggccattgtg cagattaccc atagtaggca ccagcgccac gcgcttgcct
     7441 tccatacgca ggcggcgaat ttgctgacgc agcagcggca gggtttcgat aattaacaca
     7501 acgtgactcc ttaatggaaa ctgtgttctt cgcccggata aacgccggac tccacttcag
     7561 ccatatactg ccgcacagcc gcgcggatgt cgcccgtttc ggcgaggaaa tttttagcga
     7621 atttaggaat gtgaccgccg gtaataccaa aggcgtcgtg catcacgagg atttgcccgt
     7681 cagtgacgtt gcctgcacca ataccaataa ccgggatcgc cagtgcttcg gtaatacgtt
     7741 ttgccagctc aaccggcacg cattccagca ccagcagctg tgcccccgcg gcttctaagg
     7801 ctaatgcatc gctgagtagt cgatcgctcg cttcattgcc gcgcccctgt actttgtagc
     7861 caccgaaaat attcactgac tgtggggtta aacctaagtg accacataca ggaacggcac
     7921 gttcggtcag catttttacg gtttcgacca gccactcacc gccctcaatt ttgaccatat
     7981 tggcaccggc acgcataacc gttgcggcgt tttcaaaggc ttgttccggc gtggcatacg
     8041 ccataaacgg caggtcagcc aatagcaggc agtttggtgc gccgcgacgt acggcggcag
     8101 tgtggtaggc gatatcagca acggttactg gcagagtgga atcgtggccc tgaacggtca
     8161 tgcccagcga gtcgcctacc agcatgacgt taagcccttc atcagcaaat agtttcgcga
     8221 agctgtagtc gtaagcggtg atggtcgcga aacgcttttt atcctgctta tatttctgaa
     8281 gtgaagcaat ggtggtcggt ttcataacgt atcctgataa atgatgtcgt gttgcctggc
     8341 attttatcag tcacattggt gggggcaatg atttatcaat taccgcaccg ccagatgatg
     8401 gcggtgcggt ggacttatta agggtaggtt acctggaaag tactggtggc tttaaactca
     8461 ccagcttcta ttggtatagt tccatcctgt tgtagcgtgg cctggaaatg gagagggtat
     8521 gttatacctg tatcttgatc cgggtaaact cctcctgttg tatcgtcttt gcctctcggg
     8581 tcgtaatctt tataaacaga atttgaatca ttaggtttca atgtcattag aggattttta
     8641 ctagttgcta aaccttctat gagtacaccg actccttttg ctgcatcatt acctgtgaga
     8701 gtattaccaa ggagttgccc gttttcagta ccaaccttt
//
LOCUS       DAZSIO010000157         8679 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZSIO010000157 DAZSIO010000000
VERSION     DAZSIO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.159"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(42..134)
                     /locus_tag="ACHX5T_003339"
                     /pseudo
     CDS             complement(42..134)
                     /locus_tag="ACHX5T_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(813..2009)
                     /gene="hemY"
                     /locus_tag="ACHX5T_003340"
     CDS             complement(813..2009)
                     /gene="hemY"
                     /locus_tag="ACHX5T_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR8468403.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(2012..3217)
                     /gene="hemX"
                     /locus_tag="ACHX5T_003341"
     CDS             complement(2012..3217)
                     /gene="hemX"
                     /locus_tag="ACHX5T_003341"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR8468404.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(3239..3979)
                     /gene="hemD"
                     /locus_tag="ACHX5T_003342"
     CDS             complement(3239..3979)
                     /gene="hemD"
                     /locus_tag="ACHX5T_003342"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR8468405.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3976..4917)
                     /gene="hemC"
                     /locus_tag="ACHX5T_003343"
     CDS             complement(3976..4917)
                     /gene="hemC"
                     /locus_tag="ACHX5T_003343"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR8468406.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5304..7850
                     /gene="cyaA"
                     /locus_tag="ACHX5T_003344"
     CDS             5304..7850
                     /gene="cyaA"
                     /locus_tag="ACHX5T_003344"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR8468407.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7890..8210)
                     /gene="cyaY"
                     /locus_tag="ACHX5T_003345"
     CDS             complement(7890..8210)
                     /gene="cyaY"
                     /locus_tag="ACHX5T_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR8468408.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8258..>8679)
                     /locus_tag="ACHX5T_003346"
     CDS             complement(8258..>8679)
                     /locus_tag="ACHX5T_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFR8468409.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREGNVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1975 a         2241 c         2235 g         2228 t
ORIGIN      
        1 ttaagtgata ctggttgtct ggagattcag ggggccagtc tagcagttga cggggtatct
       61 gctgcgctgg ccatgagagg aagagcggcg gcgacagcaa caacaagacg tttgggtgaa
      121 aacgaaaatt ccatgcaaaa tgctccggtt tcatgtcatc aaaatgatga cgtaattaag
      181 cattgataat tgagatccct ctccctgaca ggatgattac ataaataata gtgacaaaaa
      241 taaattattt atttatccag aaaatgaatt ggaaaatcag gagagcgttt tcaatcctac
      301 ctctggcgca gttgatatgt aaggcaggtt tattatatcg cgttgattat tgatgctgtt
      361 tttagtttta acggcaatta atatatgtgt tattaattga atgaatttta tcattcataa
      421 taagtatgtg taggatcaag ctcaggttaa atattcactc aggaagttat tactcaggaa
      481 gcaaagagga ttacagaatt atctcataac aagtgttaag ggatgttatt tcccgattct
      541 ctgtggcata ataaacgagt agatgctcat tccatctctt atgttcgcct tagtgcctca
      601 taaactccgg aatgacgcag agccgtttac ggtgcttatc gtccactgac agatgtcgct
      661 tatgcctcat cagacaccat ggacacaacg ttgagtgaag cacccacttg ttgtcataca
      721 gacctgtttt aacgcctgct ccgtaataag agcaggcgtt tttttatgca tcagaaaggt
      781 cccggaggtg ctttcctccg ggtgagaagg aactactgtg gcgggttatt ctgcaacgtt
      841 aacatcaaac cgtcgcgacg catcgctgca gcttcttccg gcttgtgcag tctgtccagc
      901 gcgtcggcaa gccatgcgta atcgtaggcg tccggacgtt gtttcagcgc cgcgcggaag
      961 gcgagcgatg cttcctgcca ttcaccgtgc ttcatcagtg actgacccaa tgtgctccac
     1021 aacagcgggc gatcaccgac gtttttgatt tgctggcgta gcactttttc cagctgttcc
     1081 gggttattgg ttttcagacg cgggatcggc agcagtaggc gatcgtcata ctggcgtttc
     1141 aggccgtcga tgataatttg ctgggcagta tcatgatcgt cacattcaat aagatgttcc
     1201 gccattgcca cctgcaacgc tacctgatga cgcgttttcc ggctttggtt tttccaccag
     1261 ttacgcaaac cttcactacc gttatcggca cgcgcctgat ccatcaggcc aatccatgcc
     1321 tgttgttcca gcattgcacg atgttcttca tcaccaacat gggctttcgc cattgatggg
     1381 atgatatcca gcagagaact ccatgcccct gtgcggatat acgcctgttc cgccagacgt
     1441 aatacttcag gatggcgtgg cgtaacttcc agcagcttat ccacgccgtg gcgtgcagca
     1501 tggttttcat tacgggccag ttgcagacgt acacgggtga tttctaccgg aatggtgtca
     1561 ttgccggcca gctccgctgc gcgttccaga tgttggttgg cgcgtgcttc atcaccacgt
     1621 tgttgcgcgg cttcggcagc cagtagatag ttcaccaccg gttgttcagc gtgatcggca
     1681 tttttcgcca tcagcttttc aacttgctga taatcacctt ccgccagttt cagcagcgcc
     1741 tgttcggtct gcttacgcgc acggcgacgc ttacgtccga caaaccatcc acgggtgtgc
     1801 gcgccagtgc ggaagatccg ccgcagtagc cactcaatgg caaacagcac taccatcgcc
     1861 agaatcaata tgatcgccag gcccgtgacg ctggtttcga tattgtagtt gtcggtctgg
     1921 atcagcacat aaccctgatg accggcaatc atcgggccaa ccactatccc cgcaatcagc
     1981 aaaacaaaga gtaataacac ttttagcatg gttattctcc ttgcggcgcg gctgccggag
     2041 tatcaccttg cggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt ttagcttccg
     2101 ttgtccccgc tgccggttgt gccagcaggt tacgcacgcg agtctgcatc agtttttcca
     2161 gcatcgcctg gctttgcagg gtttccggaa gatccatcga gatattttgc tggcttaact
     2221 ggtccacctc gtcgaggaac gctttggtgg tggcatcatc agtatcgtag taagcacgta
     2281 cccaggtgga gacgttctcc agcgcctggc gataagtctc ttcctgatga cgcggtacag
     2341 cttgtgctgc gaccagcagg cgagagcgaa tattttcgcg cagatagata tcctgatttg
     2401 gcgctaacag cggtacggcg gtgtcatcac gacggcgaat cgtaatgaag ttgtccataa
     2461 agttctgcca gcttttttgc agattgatac gccattcgct gatggaactg gaaagctctt
     2521 caccgtctga atccatcggc gaaccatcgc tgtcattatc ggccagacgc aggttatcta
     2581 cctgatttga aagctgatta agcttaagga tgatgccgtc ataatccacc tgcgatactg
     2641 cagaaaggct ggcgatatca tcggtaattg cccgacgaac ggtaatcaga ctcgggtcat
     2701 tcatatccgc caggctggcg tctgcacttt tcagcaacgc tgcagaggtc gtgacgtcct
     2761 gatcgctcca cagcttccgt ccggcgagtt tcaccagaaa atcggcctga gccagcagcc
     2821 aggttttagc atcgctgccg gaaatggtgg cgatcttttg ttggacttca tccaactgtt
     2881 ttgccagcgt ttcttgctga cgattcgcct gcttaagctg cgcagcttgt tgcttaataa
     2941 tgccttccag ctcggctttt tggctctcct gggctttttg caatgccgtc agttggttag
     3001 ccagggcatc gctggtggcg gtctgattga cggcctgttg tttaccccag ccatacaaac
     3061 cgacacccgc cgccagagca atagcgatag ccaccgcgct gagaatcaat gcagtattgt
     3121 tcttactctt tttttctgtt gcgacaggtt gtgacgtggt gtccacggcc tccctggtct
     3181 cttcaaccac ggcggaggtt ttttcttgtt ccgtcattat ggcttcctgt tatgagagtt
     3241 attgtaatgc ccgtaaaagc gcatcgttgt cagcgttatc ggcgacctta atgtcttgcc
     3301 agcccagttc ccgggcgagt ttcgccaaac gctcactgac gaccaatagt cgacagtgta
     3361 gtaaccagtg ctcacgatac cattgtggga tcagcgacca gagttgctgc aacatttcac
     3421 cgctggtaac aacgaccatc gtcacctcgc gggattgcca gcgcatcgct tcttctgcac
     3481 catcgtaatg gattgcgcat cgttgataac attcacaaaa agtgacctca gcaccgcgca
     3541 ccgtcagggt atccccaatt agctcacgac cgccattgcc acgtaatatc agcgcacgtt
     3601 tgcccgcaat attttgtaat tcaggtaatt gtagcaagac ttcgctgatt tcccgatcct
     3661 gtgggtagag aatcttctgt ccgcttacgg tatgtagcgc cagtgcggtg gtgcgtccaa
     3721 tggcgaaata agtaggtagt tggggccatt tacgatcttg ctgatgcagc tgtgattggg
     3781 caaaagcaac cgcgtgttgc gagagggcaa acaacagatc gctctccccc agcgctgcca
     3841 gttgatcagc aagttgcggt aattgtcgac ccggagaaaa ctcaatcagc ggaaaatgcc
     3901 aggccacctg ccccagtgtg cgcagacggc tcactaactc ttctccagcg ggagacgggc
     3961 gggtgaccag gatactcatg ccggggcctc tccgttatag acttcagcga ggatctcgcg
     4021 cgcgccgtta ttcagtagct cttctgccag cgaaatcccc atttgttcgg catcttgcgg
     4081 cgcaccgcgg cgttcaccgc gaataatctg cgaaccgtcc ggcgcgccga ccagcgcaca
     4141 tagccagatt tcgccatcaa taagctcggc gtagctacca attggcacct gacatccgcc
     4201 ttcgagacgg gtattcatgg cgcgttctgc ggtaacgcgc agtgcagttt cgtggtgatt
     4261 cagcgcggca agcagctcgc gagtgcgtgt atcatcaagg cggcattcaa taccgactgc
     4321 accttgtcct accgccggaa gagaaatctc gggtggtaac gcggcgcgaa tgcgggactc
     4381 cagacctaaa cgttttagtc cggctacggc aagaatgatg gcatcgtatt cgccgttatc
     4441 tagtttgctc aggcgagtgc cgacgttgcc gcgcagggag cggataatca gatccgggcg
     4501 gcgttcagcc agttggcact ggcgacgtaa actggacgtc ccgacgatac tgcctgctgg
     4561 taacgcatcc agactgtcat agttattgga cacaaaggca tcgcgaggat cttcacgctc
     4621 gcaaatagtg accagcccca gaccttgcgg gaattcaacc ggcacatctt tcattgaatg
     4681 tacggcgata tcagcgcgat tctccaggag cgcgacttcc agctctttta caaataagcc
     4741 ttttccgcct actttcgcca gcggcgtatc aagaatcaca tcgccgcgcg tcaccatcgg
     4801 taccagttca acgaccaggc ccggatggct cgccatcaac ttgtctttga catagtgtgc
     4861 ctgccagagt gcaagtgggc tttggcgtgt ggcaattctt aaaacattgt ctaacatgct
     4921 tgttaccgtc attatcatcc gtggtccatc ctaacatcct tgccagagtg atgtcagtgt
     4981 tgtggtgaaa cgtagacgcc tgcgcaaacc gtaaaatgag gtctggcagt ggatcctgac
     5041 aggcgtttca cgccgtcgta ataaggaatt tacagagaat aaacggtgct acacttgtat
     5101 gtagcgcatc tttctttacg gtcaatcagc aaggtgttaa attgatcacg ttttagacca
     5161 ttttttcgtc gtgaaactaa aaaaaccagg cgcgaaaagt ggtaacggtt acctttgaca
     5221 tacgaaatat cccgaatgcc gcgtgttacc gttgatgttg gcggaatcac agtcatgacg
     5281 ggtagcaaat caggcgatac gtcttgtacc tctatattga gactctgaaa cagagactgg
     5341 atgccataaa tcaattgcgt gtggatcgcg cgcttgctgc tatggggcct gcattccaac
     5401 aggtctacag tctactgccg acattgttgc actatcacca tccgcgaatg ccgggttacc
     5461 ttgatggtaa cgttcccaaa ggcatttgcc tttacacgcc tgatgaaact caacgccact
     5521 acctgaacga gcttgaactg tatcgtggaa tgtcagtaca ggatccaccg aaaggtgagc
     5581 ttccaattac tggtgtatac accatgggca gcacctcgtc cgtagggcaa agttgttcct
     5641 ctgacctgga tatctgggtc tgtcatcaat cctggctcga tagcgaagag cgccagttac
     5701 tacaacgtaa atgcagcctg ctggaaagct gggccgcctc gctgggtgtg gaagtcagct
     5761 tcttcctgat tgatgaaaac cgcttccgtc ataatgaaag cggcagcctg gggggcgaag
     5821 attgtggctc cacccagcat atactgctgc ttgacgaatt ttatcgtact gccgtgcgtc
     5881 tcgccggtaa gcgtattctg tggaatatgg tgccgtgcga cgaagaagag cattacgacg
     5941 actatgtgat gacgctttac gcgcagggcg tgctgacgcc aaatgaatgg ctggatctcg
     6001 gtggcttaag ctcgctttct gctgaagagt actttggtgc cagcctttgg cagctctaca
     6061 agagtatcga ttccccatac aaagcggtgc tgaaaacact gctgctggaa gcctattcct
     6121 gggaataccc gaacccacgt ctgctggcga aagatatcaa acagcgtttg cacgacggcg
     6181 agattgtatc gtttggcctc gatccatact gcatgatgct ggagcgtgtt actgaatacc
     6241 tgacggcgat tgaagatttc acccgtctgg atttagtacg tcgctgcttc tatttaaaag
     6301 tgtgcgaaaa actcagccgt gaacgcgcct gtgtaggctg gcgtcgcgca gtgttgagcc
     6361 agttagtgag cgagtggggt tgggacgaag ctcgtctggc aatgctcgat aaccgtgcta
     6421 actggaagat tgatcaggtg cgtgaggcgc acaacgagct gctcgacgcg atgatgcaga
     6481 gctaccgtaa tctgatccgc tttgcgcgtc gcaataacct tagcgtctcc gccagtccgc
     6541 aggatatcgg cgtactgacg cgtaaactgt atgccgcgtt tgaagcattg ccaggtaaag
     6601 tgacgctggt aaacccgcag atttcacccg atctctcgga accgaatctg acctttattt
     6661 atgtgccgcc gggccgggct aaccgttcag gttggtatct gtataaccgc gcgccaaata
     6721 ttgagtcgat catcagccat cagccgctgg aatataaccg ttacctgaat aaactggtgg
     6781 cgtgggcatg gtttaacggc ctgctgacct cgcgcacccg tttgtatatt aaaggtaacg
     6841 gcattgtcga tttgcctaag ttgcaggaga tggtcgccga cgtgtcgcac catttcccgc
     6901 tgcgcttacc tgcaccgaca ccgaaggcgc tctacagccc gtgtgagatt cgccatctgg
     6961 cgattatcgt taacctggaa tatgacccga cagctgcgtt ccgcaatcag gtggtgcatt
     7021 ttgatttccg taagctggat gtcttcagct ttggcgagaa tcaaaattgc ctggtaggta
     7081 gcgttgacct gctgtaccgc aactcgtgga acgaagtgcg tacgctgcac ttcaacggcg
     7141 agcaatcgat gatcgaagcc ctgaaaacta ttctcggcaa aatgcatcag gacgccgcac
     7201 cgccagatag cgtggaagtc ttctgttata gccagcatct gcgcggctta atccgtactc
     7261 gcgtgcagca actggtttct gagtgtattg aactgcgcct ttccagcacc cgccaggaaa
     7321 ccgggcgttt caaggcgctg cgcgtttctg gtcaaacctg gggcttgttc ttcgaacgcc
     7381 tgaatgtatc ggtacagaag ctggagaacg ccatcgagtt ttatggcgct atttcgcata
     7441 acaaactgca cggcttgtca gtgcaggttg aaaccaatca cgtcaaatta ccggcggtgg
     7501 tggacggctt tgccagcgaa gggatcatcc agttcttttt cgaagaaacg caagacgaga
     7561 atggctttaa tatctacatt ctcgacgaaa gcaaccgggt tgaggtgtat caccactgcg
     7621 aaggcagcaa agaggagctg gtacgtgacg tcagtcgctt ctactcgtca tcgcacgacc
     7681 gcttcaccta cggctcaagc ttcatcaact tcaacctgcc gcagttctat cagattgtga
     7741 aggttgatgg tcgcgaacag gtgattccgt tccgaacaaa atcgattggc aacctgccgc
     7801 ccgccaatca ggatcacgat acgccgctat tacagcagta tttttcgtga tgagcgtgcc
     7861 ggaaagcgag gcttatccgg catgcaatct tagcggaaac tgactgtttc acccgcctgt
     7921 tgcgtcgccg cctgttccag caaatcccag aaggtttcgc cgctgcgatc gcaaatccat
     7981 tcatcgcctt tcaggtcaaa atggtagccg ccctgtttgg ttgccagcca cacctggtgc
     8041 agcggctcct ggcggttaat aatgattttg ctgccgtttt caaaggtaat ggtcagtacg
     8101 ccgccgttga tttcgcagtc gatatcgctg tcgccatccc tgtcgtccag acgctcttca
     8161 atggtcagcc acagttgatc agccaggcga tgaaattcac tgtcgttcat tgttgtatcc
     8221 tgtttttaag tgatggcggc agtatagcgg catggggtca gggcttcaaa gtttgcacct
     8281 ctgctgctgc gttccggcat gattcatccg tcaccggaat aatgatgtct ctgttttgaa
     8341 cttcacgtac gttcccctct ctgacacgag ttttcgccgg gcagggggag ggcgtaatcc
     8401 ttacctctgt atcggtccat gtataaacat tgcgatcgtc ataagcgaag cgatcgtttt
     8461 tagttagcga accgttaact ggaataattt gcaagctctt tgaccgtaat tggcggataa
     8521 agtaagcatg ctgtttgccg ctattaaatg agcgatcaat gaccaggtct ggcgtatcca
     8581 gcgggattat ctcaagctgg tcttcaccaa atgtcgataa ttttgttaca tacagccgat
     8641 ctttatattg cgcaatattg ccagtaatca gatggaatt
//
LOCUS       DAZSIO010000158         8625 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZSIO010000158 DAZSIO010000000
VERSION     DAZSIO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.160"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(341..547)
                     /locus_tag="ACHX5T_003347"
     CDS             complement(341..547)
                     /locus_tag="ACHX5T_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR8468410.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            860..949
                     /gene="kdpF"
                     /locus_tag="ACHX5T_003348"
     CDS             860..949
                     /gene="kdpF"
                     /locus_tag="ACHX5T_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR8468411.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            949..2622
                     /gene="kdpA"
                     /locus_tag="ACHX5T_003349"
     CDS             949..2622
                     /gene="kdpA"
                     /locus_tag="ACHX5T_003349"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR8468412.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2645..4395
                     /gene="kdpB"
                     /locus_tag="ACHX5T_003350"
                     /pseudo
     CDS             2645..4395
                     /gene="kdpB"
                     /locus_tag="ACHX5T_003350"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4701..5273
                     /gene="kdpC"
                     /locus_tag="ACHX5T_003351"
     CDS             4701..5273
                     /gene="kdpC"
                     /locus_tag="ACHX5T_003351"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR8468413.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5266..7946
                     /gene="kdpD"
                     /locus_tag="ACHX5T_003352"
                     /pseudo
     CDS             5266..7946
                     /gene="kdpD"
                     /locus_tag="ACHX5T_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            7943..8625
                     /gene="kdpE"
                     /locus_tag="ACHX5T_003353"
                     /pseudo
     CDS             7943..8625
                     /gene="kdpE"
                     /locus_tag="ACHX5T_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
BASE COUNT         1837 a         2232 c         2494 g         2062 t
ORIGIN      
        1 cggtagcgat actaataaca aaattcggaa tttatttaga tgctggaatt agaaaaaggg
       61 atgacggctt tctcacctta ccctgaagtt tcacgaaagt gtgtaaggaa gtatgcaatc
      121 taactggaga tttctgatga tctagtcgct tagagcgaca atgaaggtga ccgcatcctg
      181 ggtagcccta tgaaaggata ctattgaaac gtgatgctaa aacagtgatg aaatgaataa
      241 tacgatggaa acgtcttcca tgttaattat catggaagac gttgatgttg aacacctaaa
      301 ccagccccct gatacgaggc tggcttaagc gcgcaggcaa tcagtaaaaa tcaccggttg
      361 ccttttcggc ctgatccatc cacaccggtt tatcgctggt tttcgaccag acgcggtgca
      421 ggtagctgta aaagcgggcg cggtctttcc agaaaagcat gaaaggcagc gccagaacgc
      481 ccgctgcaac cgcatatgta cggcgtaaaa agataagcca tgcaggatat tctctgtaga
      541 gttccatact tttctccccc ttttgtatct acccggtgaa tagcaccgga aaaatgaatt
      601 tgtttatctg atgaaaatag taccgccttt tgtgtaattt tactactcat ccgaccactt
      661 atttttgctt attgatggtt tatttacatt cacactgtaa ttaaattaca caaaagttaa
      721 attaatacta accatcagtt aaatcatggc ctttgccatt tttatacttt ttttacaccc
      781 cgtccgcaga tttttgcgaa atctttgcag ccagaattct actcttccgg tatcactttt
      841 aggccactgg aggtgcactg tgagtgcagg cgtgatagcc ggcgtgttgc tggtgttttt
      901 attactgggc tatttggttt atgccctgat caatgcggag gcgttctgat ggctgcgcaa
      961 gggttcttac tgattgccac gtttttactg gtattaatgg tgctggcgcg tcctttaggc
     1021 agcgggctgg cgcggctgat taatgacatt cctcttccag gtacagcggg cgttgaacgt
     1081 atactctttc gtttacctgg cgtctctgac catgagatga actggaagca atatctttgt
     1141 gccattctcg gcctgaacat gctggggctg gcggtgctgt tttttatgtt gctcggtcag
     1201 cactatctgc cgcttaatcc acagcagttg ccggggctgt cgtgggatct ggcgctgaat
     1261 accgccgtca gctttgtcac gaataccaac tggcaatctt atagcggtga aaccacgttg
     1321 agctatttca gccagatggc aggcttaacg gtacaaaact ttctttctgc cgccagcggg
     1381 attgcggtga tttttgcctt catccgtgcg tttactcgcc agagcatgag cacactcggg
     1441 aatgcctggg tcgatctgct acgcatcacg ttatgggtgc tagtccctgt ggcgttgttg
     1501 atcgcactgt tttttattca acaaggtgcg cagcaaaact ttctgcccta tcaggcagtg
     1561 aataccgttg aaggagcgca acagctgtta cccatgggac cagtagcttc tcaggaagcg
     1621 atcaagatgc tcggtactaa cggcggtggc ttctttaatg ccaactcgtc gcatccgttt
     1681 gaaaacccaa ccgcactgac caacttcgtg cagatgctgg cgatcttctt gatcccaaca
     1741 gcgctgtgct ttgcctttgg tgaagtgacg ggcgatcgcc gccaggggcg catgttgctg
     1801 tgggcgatgt cagtgatttt tgtcatctgc gtaggcgtgg ttatgtgggc agaagttcag
     1861 ggtaatcctc atctgctggc gctcggcgca gacagcagca tcaatatgga aggtaaagag
     1921 agccgtttcg gcgtgctggt cagtagcctg tttgcggtcg tgactacggc ggcgtcctgt
     1981 ggcgcggtga ttgcgatgca tgattcgttt accgctctcg gcggcatggt gccgatgtgg
     2041 ctaatgcaaa ttggtgaagt ggtgttcggc ggtgtcggtt ctggtcttta cggcatgatg
     2101 ctgtttgtcc tgctggcggt gtttattgcc gggctgatga ttggtcgtac accggaatat
     2161 ctgggtaaaa aaatagacgt acgcgagatg aaactgactg cactggcgat tctggttact
     2221 ccaacactgg tactgatggg cgcggcgttg gcgatgatga ccgacgccgg acgtagcgcc
     2281 atgcttaacc ctagcccgca tggttttagc gaagtgctgt acgccgtgtc gtccgccgct
     2341 aacaacaacg gcagcgcctt tgccggatta agcgccaact ctccgttctg gaactgttta
     2401 ctggcgttct gcatgtttgt cggtcgcttc ggggtgatta tcccggtgat ggcaattgcc
     2461 ggttcgctgg tgagtaaaaa gagccaaccc gccagctccg gcacgctgcc aacgcacggc
     2521 ccgctgtttg ttggcctgtt aatcggcacc gtgttgctgg ttggcgcact gacctttatc
     2581 cctgccctgg cgcttggtcc ggtggcggaa tatctctcct gatgatattg agtgagcact
     2641 gaatatgagt cgtaaacaac tggcgctatt cgaaccaaca cttgtcgttc aggcgctgaa
     2701 agaagcggtg aaaaaattaa acccgcaggc gcaatggcgc aatccggtga tgtttatcgt
     2761 ctggatcggc agtctgctga ccacctgtat tagcatcgcg atggcaagcg gtgcgatgcc
     2821 tggcaatgcg ctgtttagcg cggcaattag cggctggctg tgggtcaccg tactgttcgc
     2881 taatttcgcc gaggcgctgg cagaaggccg cagtaaagcg caggccaaca gtcttaaagg
     2941 ggtgaaaaaa actgcctttg cccgcaagct gcgtgagccg aaatatggcg ctgcggcgga
     3001 caaagttcct gccgatcaac tgcgtaaagg tgacatcgtg ctggtagaag ctggcgatat
     3061 tatcccctgc gatggtgaag tcattgaagg cggcgcatcg gtcgatgaaa gcgccatcac
     3121 cggggaatcg gcaccggtga tccgtgaatc cggcggcgat tttgcctccg ttaccggcgg
     3181 cacgcgtatt ctttctgact ggctggtgat tgagtgtagc gttaaccccg gtgagacatt
     3241 tctggatcgg atgatcgcga tggtggaagg cgcacagcga cgcaaaacac cgaacgagat
     3301 tgccctgact atcctgctga ttgctctgac catcgtcttt ttactggcaa ccgccacgct
     3361 gtggccgttt tccgcgtggg gcggtaatgc cgtgagtgta acggtactgg tggcgctgct
     3421 ggtctgtctg atcccaacca ctattggtgg cctgttgtca gcgatcggcg tcgccgggat
     3481 gagtcggatg ctgggcgcga atgtgattgc caccagcgga cgtgcagttg aagcggcagg
     3541 tgacgttgac gttctgctac tggataaaac cggcaccatc acactcggta accgtcaggc
     3601 gtcggagttt atccccgcgc agggcgtgga tgaaaaaacg ctggctgacg ccgcacaact
     3661 ggcttcgctg gctgatgaaa cgccggaagg tcgcagtatt gtgatcctcg ccaagcaacg
     3721 ttttaacctg cgcgagcgcg atgtgcagtc gctccatgcc acctttgtac cgtttactgc
     3781 gcaaagccgg atgagcggga tcaacatcga caaccgcatg atccgtaaag gttctgtcga
     3841 tgccattcgc cgccatattg aggctaacgg cggtcacttc cctgccgatg ttgatcaaaa
     3901 agtcgatcag gttgcgcgtc aaggagccac gctgctggtg gtggtggaag gttctcgtgt
     3961 gctgggcgtt attgcgctga aagatatcgt caaaggcggt attaaagagc gcttcgccca
     4021 gctgcgcaaa tgggcattaa aacggtgatg attaccggcg ataaccgtct gactgccgcc
     4081 gcgatagctg cggaagcggg tgtcgatgat tttctcgccg aagcgacacc ggaggccaag
     4141 ctggcattga tccgtcagta tcaggcggaa ggtcgtttgg tagcgatgac cggcgacagc
     4201 accaacgatg ctacggcgct ggcgcaggca gatgtcgcgg tggcgatgaa ctccggcacc
     4261 caggcggcta aagaggcggg caatatggtg gatctcgact ctaacccgac caagttgatc
     4321 gaggtggtgc acattggcaa acagatgctg atgacccgtg gctcgctgac caccttcagc
     4381 attgccaacg atgtggcgaa atacttcgcc attattccgg cggcgttcgc ggcaacatat
     4441 ccgcagttaa atgcgctgaa catcatgcgc ctgcattcgc ccgactccgc cattctcagt
     4501 gcggtcattt tcaacgcctt gattatcgtc tttttgattc ctctggcgtt aaaaggcgtg
     4561 agttataaac cgcttaccgc ttctgccatg ttgcgccgta acttatggat ttacggtctg
     4621 ggtgggctgc tggtgccgtt tattggtatc aaagtcattg atttactgct aaccgtttgt
     4681 agtctggtgt gaggtttacc atgagtggat tacgtccggc attatcgaca tttctctttc
     4741 tgttattgat tactggcggc gtttacccgc tgctgaccac cgcactgggg caatggtggt
     4801 atccctggca ggccaacggt tcgttgattc gtgaaggtga tacggtgcgc ggttcggcat
     4861 taatcgggca aaattttacc ggcaacggct attttcatgg tcgcccgtcg gcaacagcag
     4921 aaatgcccta taatccacag gcttctggcg ggagcaatct ggcagtcagt aacccggagc
     4981 tggataaaca gatagccgca cgcgttgccg cattacgggc cgctaacccg gacgccagca
     5041 cgaacgttcc ggttgaactg gtgacggcgt cggcaagcgg actggacaat aacatcaccc
     5101 cgcaagcggc ggcctggcag atcccacgca tcgcaaaagc gcgtaatctc agcgttgaac
     5161 agctcacgca gctgatcgca aaatacagcc aacaaccgct ggtgaaatat atcggccagc
     5221 cggtcgtcaa cattgtcgaa ctcaatctgg cactggataa gcttgatgaa taacgaaccc
     5281 ttacgtcccg accccgaccg tctgctggaa caaactgccg cgccgcatcg ggggaagctg
     5341 aaagttttct tcagtgcctg tgcaggcgtc gggaagacct gggcgatgct ggcagaagcc
     5401 cagcgactgc gggcgcaagg gctggatatt gtggttggtg tggtagaaac ccatgggcgg
     5461 aaagataccg ccgccatgct tgaagggctg gctgttctgc ctctgaaacg ccaggcgtac
     5521 cgtgggcggc atatcagcga gtttgatctc gatgccgccc tcgcccgccg tccggcgctg
     5581 atcttaatgg acgaactggc gcacagtaat gcgccaggtt ctcgtcatcc taaacgctgg
     5641 caggatatcg aagaactgct ggaagctggc attgatgttt tcacaaccgt caacgttcag
     5701 catctggaaa gtctgaatga tgtggtcagc ggcgtcaccg gaattcaggt acgggaaacc
     5761 gtgcccgatc cttttttcga tgccgccgac gacgtggtgc tggtggactt gcccccggac
     5821 gatctgcgcc agcggctgaa agaaggcaaa gtctatattg ccgggcaggc ggagcgcgcc
     5881 attgaacatt ttttccgcaa aggcaatctg atcgccctgc gcgaactggc actgcgccgt
     5941 actgccgatc gcgttgatga gcaaatgcgc gcctggcggg ggcatcctgg cgaagagaaa
     6001 gtctggcaca cgcgcgacgc gatcctttta tgcatcggcc ataacaccgg cagcgaaaac
     6061 tggtccgcgc ggcggcgcgg ctgacgtcac ggctggggag cgtctggcac gcggtgtatg
     6121 ttgaaacccc tgccctgcat cgcttaccgg agaaaaaacg tcgggcaatt ctcagcgcct
     6181 tacgtctggc gcaggagctg ggcgcggaga cggcaacact ttctgatcca gcggaagaga
     6241 aagcggtagt gcgttatgcc cgtgaacata atctcggcaa gattattctc ggtcgcccag
     6301 cctcgcgccg ctggtggcgt cgggaaacat ttgctgaccg actggcgcgc atcgcccccg
     6361 atctcgatca ggtgctggtc gcgcttgatg aaccacccgc ccgcacgatt aacaacgcgc
     6421 cggatagccg ctcatttaaa gacaagtggc gggtacagat tcagggatgc gtggttgccg
     6481 ccgcgttatg cgccgttatc accttaattg ccatgcagtg gctgatggcg tttgatgccg
     6541 ccaacctggt gatgctgtat ctgcttggcg tggtggtggc gctattttat ggacgctggc
     6601 cttcggtggt tgccaccgtc attaatgtgg tgagtttcga tctctttttt atcgccccac
     6661 gcggcacgct cgccgtctct gatgtgcaat atctgctgac cttcgcggtg atgttaaccg
     6721 tcgggctggt gatcgggaac cttactgctg gtgtgcgtta tcaggcgcgg gtagcccgtt
     6781 accgcgagca acgcacacgg cacttatatg aaatgtcgaa agctctggcg gtgggccgca
     6841 gtccgcagga catcgccgcc accagcgaac aatttattgc ctccacgttt catgcccgca
     6901 gtcaggtgtt gttgcccgat gacaacggta aattgcagcc gttaacacat ccgcaaggaa
     6961 ttacgccatg ggacgatgcc atcgcgcagt ggagttttga taaaggcctg cctgcgggcg
     7021 cgggcaccga cacgttaccc ggtgtaccgt accagatttt gccgctaaaa agcggcgaga
     7081 aaacctacgg gctggtggtg gtggagccgg ggaatctgcg ccagttgatg atcccggaac
     7141 agcagcgcct gctggagact tttacgctgt tagtcgccaa tgcccttgag cggctgacgc
     7201 taaccgccag cgaagaacag gcgcggatgg caagcgaacg tgaacagatc cgcaacgccc
     7261 tgctggcagc gctttcgcat gatttacgca cgccgcttac ggtgctgttt ggtcaggcag
     7321 aaatcttaac gctcgatctg gcaagcgaag gatcacccca cgcccgccag gccagcgaga
     7381 ttcgtcagca tgtgctgaac actacccgac tggtgaataa tctactggat atggcgcgaa
     7441 ttcagtccgg cggctttaat ttgaagaaag agtggttaac gctggaagaa gtagtcggca
     7501 gcgcgctgca aatgccggaa ccgggtttat cgtcgcccat caatctttct ctgccagaac
     7561 cgctgacctt aatccacgtt gacgggccac tctttgaacg ggtgctgatt aatctgctgg
     7621 agaacgcggt gaaatatgcg ggtgcgcagg ccgaaattgg tatcgatgcc cacgttgagg
     7681 gcgaaaatct acaactggat gtctgggata acggccccgg tcttccgcca ggccaggagc
     7741 agacgatatt tgataagttt gctcgcggga ataaagagtc ggcggtgccg ggtgtcgggc
     7801 ttgggttggc aatttgtcgg gcgattgtgg atgtacacgg cggcaccatt accgcgttca
     7861 accgaccgga aggtggtgcc tgttttcgtg ttacacttcc ccagcaaacc gctcctgaac
     7921 ttgaagactt tcatgaggat atgtgacaaa cgttctgatt gttgaagatg aacaggctat
     7981 tcgtcgcttt ctgcgcacgg cgctggaggg cgacgggatg cgcgtctttg aggccgaaac
     8041 tctgcaacgc ggcttgctgg aagcggcaac ccgtaagcca gatttgatta ttctcgatct
     8101 cggcctgccc gatggtgatg ggattgagtt tatccgcgac ctgcgccagt ggagcccggt
     8161 gccggtgatt gtgctttccg cacgcagcga agagagcgac aagatcgccg cgctggatgc
     8221 cggagcggat gattatctga gtaagccgtt tggtattggc gaattgcagg cccgtctgcg
     8281 cgtcgcttta cgccgccact ccgccactcc gccaccgccg cgcccgatcc gctggtgaaa
     8341 ttttccggtg ttaccgtcga tttagccgcc cgcgtgattc accggggtga ggaagaggtg
     8401 catctcacac caattgagtt ccgcctgctg gcggtgctgc tcaacaatgc cggaaaagta
     8461 ctcacccagc gccagctcct taaccaggtg tgggggccaa acgcggtcga acacagtcac
     8521 tatttgcgta tttatatggg acatctgcga caaaaactgg aacaggatcc ggcccggcct
     8581 cgccacttca ttactgaaac cggtattggg tatcggttta tgctt
//
LOCUS       DAZSIO010000159         8528 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZSIO010000159 DAZSIO010000000
VERSION     DAZSIO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.161"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..189
                     /gene="csgC"
                     /locus_tag="ACHX5T_003354"
     CDS             <1..189
                     /gene="csgC"
                     /locus_tag="ACHX5T_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR8468414.1"
                     /translation="GSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVS
                     DGQALHLSQQWTPSSEKS"
     gene            310..621
                     /gene="ymdA"
                     /locus_tag="ACHX5T_003355"
     CDS             310..621
                     /gene="ymdA"
                     /locus_tag="ACHX5T_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR8468415.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            716..1249
                     /gene="ymdB"
                     /locus_tag="ACHX5T_003356"
     CDS             716..1249
                     /gene="ymdB"
                     /locus_tag="ACHX5T_003356"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR8468416.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1191..2672
                     /gene="clsC"
                     /locus_tag="ACHX5T_003357"
     CDS             1191..2672
                     /gene="clsC"
                     /locus_tag="ACHX5T_003357"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR8468417.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2680..3837)
                     /gene="mdoC"
                     /locus_tag="ACHX5T_003358"
     CDS             complement(2680..3837)
                     /gene="mdoC"
                     /locus_tag="ACHX5T_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR8468418.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4231..5766
                     /gene="mdoG"
                     /locus_tag="ACHX5T_003359"
     CDS             4231..5766
                     /gene="mdoG"
                     /locus_tag="ACHX5T_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR8468419.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5759..8302
                     /gene="mdoH"
                     /locus_tag="ACHX5T_003360"
     CDS             5759..8302
                     /gene="mdoH"
                     /locus_tag="ACHX5T_003360"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR8468420.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2150 a         2025 c         2222 g         2131 t
ORIGIN      
        1 ggcagttcag ggcaaagtca gacgaagcaa gaaaagaccc tctcattgcc tgctaatcaa
       61 cccattgctt tgacgaagtt gagtttaaat atttccccgg aagatcgggt gaaaatagtt
      121 gttactgttt ctgatggaca ggcacttcat ttatcacaac aatggacgcc ctcttcagaa
      181 aagtcttaat ttgttgaaat atcgagcata agatgaatct ggagagaatg gtctgctgcg
      241 aatcagccaa cctgaaagta tggataacac aaccttcaag gatgactaat cattgaggaa
      301 atagaataaa tgttcagacc ttttttagac tctcttatgc tcggcagttt gttttttcct
      361 tttattgcca ttgcaggaag caccgcgcaa gggggcgtga tccattttta tggccaaatt
      421 gtggaaccgg catgtgacgt cagcacccag tcatcacccg tagaaatgaa ctgcccacag
      481 aatggttcta ttccgggcaa aacctactcc agcaaagcgt taatgagcgg caatgtcaaa
      541 aacgcgcaaa tagcctcagt aaaagtgcag tatcttgata aacagaaaaa gctggctgta
      601 atgaacatcg aatataacta agtttctgaa caactcacgt tgctgagcag aaaaatgcga
      661 tttaaccaaa aagcctgctg tacacttaag aaacaagaag gtgtaaggag gcgttatgaa
      721 aacgcgtatt catgttgtgc agggtgatat taccaaactg gccgttgatg tgattgtgaa
      781 tgcggctaat ccgtcattaa tgggaggcgg cggcgtcgat ggggccattc atcgcgcagc
      841 gggtccggcc ctgctggatg cctgtttaaa agtcaggcaa cagcagggcg attgccctac
      901 ggggcatgcc gttattacgc ttgcaggcga tcttcccgct aaagccgtag tacacaccgt
      961 cgggccagtc tggcgtggtg gggaacaaaa cgaagaccag cttttgcagg atgcctatct
     1021 gaatagccta cgactggtgg cggcaaacag ctatacgtca gtggcttttc ctgcaatcag
     1081 tactggggtt tatagttacc ctcgtgcggc agcggctgaa atcgcagtta aaaccgtttc
     1141 agaatttatt acccgtcacg ctttacccga acaggtatac tttgtctgtt atgatgaaga
     1201 aaacgcccac ctctacgaaa gactccttac ccaacaagga gatgaatgat ttgccccggc
     1261 tggcgagcgc ggtgctgcca ctgtgttcgc aacatcccgg tcagtgtggt cttttccctc
     1321 tggagaaaag tctggatgcg tttgccgccc ggtatcgtct ggccgaaatg gcagagcata
     1381 cgctggatgt tcagtattac atctggcagg acgatatgtc ggggcggtta ctgttttccg
     1441 ccctgttagc cgcagcaaag cgtggcgttc gcgtccgttt gttgctggac gacaacaata
     1501 cgcccggact tgacgacatt ttgcgcttgc ttgacagtca tccacgcatt gaagtccggc
     1561 tttttaatcc tttctcgttt cgcttgctac gtccgcttgg atatatcacc gacttttccc
     1621 gtctcaatcg ccggatgcac aataaaagtt tcactgtcga tggcgtggtt accctagtgg
     1681 gaggacgaaa tattggtgat gcctattttg gtgcagggga agagccactt ttttcggatt
     1741 tagatgtcat ggcaataggc cccgtggtag aggatgttgc cgatgatttc gcccgctact
     1801 ggtattgcaa atcggtttca cccttacagc aggtgctgga tgtcccggaa ggtgaaatgg
     1861 cggatcgcat cgagttaccc gcctcctggc ataacgatgc catgacgcat cgttatttac
     1921 gcaaaatgga atccagtcca tttataaatc atctggttga tggaacgttg ccgcttatct
     1981 gggcgaagac acgtttatta agtgatgatc cggcgaaagg ggagggcaag gcaaaacggc
     2041 attcactgtt accgcagcgc ctgttcgata tcatgggctc acccagtgaa cgcatcgata
     2101 ttatctcttc ctattttgta ccgacacgcg caggtgtggc gcaactctta cggatggtga
     2161 gaaaaggggt aaagattgcg atcctaacca attctcttgc cgctaacgat gttgctgtcg
     2221 tccatgccgg atacgcgcgc tggcgcaaaa aattgctccg ctatggcgtg gaattatatg
     2281 aactcaagcc gacgcgtgaa caaagcagta cgttacacga tcgcggcata accggtaatt
     2341 ccggtgccag cctgcatgct aaaactttta gcatcgatgg taaaacggtg tttatcggct
     2401 ctttcaattt cgatccgcgt tcaacattgc tcaatactga aatgggcttc gtgatagaga
     2461 gcgaaacgct ggcacagtta attgataaac gctttattca gagccagtac gatgcggcct
     2521 ggcagctccg tctggacagg tggggacgga tcaactgggt tgatcgtcat gcaaagaaag
     2581 aggttattct caaaaaagaa cccgccacca gtttctggaa gcgggttatg gtcagactgg
     2641 cgtcgatatt gcccgtggaa tggttattgt aaatgtggct taacgggctg gtgctttatc
     2701 gttctcacgc ttaacaaccg gtttaccaga gaacaaaaac ttcagtaacg ggatgcgtaa
     2761 atgaatttca tacagaatta tcgcaatccc tactacgaat atcaggccac agagaaaacc
     2821 aagccagttg gaggtgatgt gcggtgtaat gtatgcgccg aaaaacagcg ttaacgggtg
     2881 gtgaaccaga tagataaaca gcgatgcgtt aacaaaataa gtcacccgcg ctgactggaa
     2941 gttaagcaaa cggtggccaa aggagaagac cacattcacc atccacaggc cgaggaccat
     3001 ggtgatcacc gactcggttt cgtacatcca ggcatcgcca ctgccatagc gctggttgag
     3061 taaataagcg acaaacgcca atgctgcagc gagggtacag ccacgagacg gcgtggtaaa
     3121 caaggcttta agatgaggga aaatgaaagc cagtgcgccg aggataaaga acggtaaata
     3181 aaatagcgtt tgcatgacaa taaaattgaa catgccatta ctcagaatgg gtggatacac
     3241 aataaaaatc gttcttctta ttaccgcata gccgatgccg aggcataaaa aaatcaccga
     3301 tagttttacc atcgagaatt ttttattcgt tttatcagaa ttttctaaat tatttctgat
     3361 gcgcttaaat atccatacac acagcgtcgt catgactact aacaccagta aaaaccacag
     3421 gtgtgatatt aattcccagg ccaacgtatt atatttgtca tacaatgata gcccaggcca
     3481 actttccgct ttccctttga catattgcag cataataaat tgcggtaatg tcagtagggg
     3541 gatggctgtt aacatcggga tacctacacg ttcgacacgt actttccacc attttttcaa
     3601 gggatagcgt aaaaaaagca tgtaggaaaa gtatccggat ataacgaaaa atacctgcat
     3661 gcggaacgag tggatgaagt cattaaaaag ggttagccac aatgacggtt cggcgctatt
     3721 cacatgccat gtatggctcg aatagattaa agaaatatga aaagggatcc ctaacaacat
     3781 cagccaggcg cggatggagt cgaggaaata ttcacgttgc gcgggtactg gattcatata
     3841 tggttaacta atctcggatt tttcgtctta tccctgtcgg gttatgcctt taggcttgtt
     3901 gccatagcga caccgacctg accgcgccag gcgcaggctt caaggttttt atgcatagca
     3961 tcatcgctac cactaaccag aatggaagcg tctgtaagac ggttgataaa taaatttgct
     4021 ggcaaaccct acacgaagtc gatgcttctg tctttaggag aagcacggaa agtgaaaacg
     4081 gttgcaatca ggtgcttaat ccatgagcca gcgtgctgaa cgataccggg attcagttgt
     4141 cggaatggct ggttatccat taaaatagat cggatcgata taagcacaca aagggggaag
     4201 tgcttactaa ttatgaaaca taaactacaa atgatgaaaa tgcgttggtt gagtgctgca
     4261 gtaatgttaa ccctgtatac atcttcaagc tgggctttca gtattgatga tgtcgcaaag
     4321 caagctcaat ccttagccgg gaaaggctat gaggcgccta aaagcaactt gccctccgtt
     4381 ttccgcgaca tgaaatacgc ggactatcag cagatccagt ttaatcatga caaagcgtac
     4441 tggaacaatc tgaagacccc attcaaactc gagttctacc atcagggtat gtacttcgat
     4501 accccggtca aaataaatga agtgactgcc accgcagtca aacgaatcaa atacagcccg
     4561 gattatttca ctttcggcga tgttcagcat gacaaagaca cggtaaaaga ccttggtttt
     4621 gccggtttta aagtgcttta cccgatcaac agcaaagata aaaacgatga aatcgtcagc
     4681 atgctcgggg ccagctattt ccgcgtgatt ggtgcaggtc aggtttatgg cctttctgcc
     4741 cgcggcctag caattgatac cgctttgcca tcgggtgaag aatttccacg cttcaaagag
     4801 ttctggatcg agcgtccaaa accgactgat aaacgtttaa ccatttatgc attgcttgac
     4861 tcgccgcgtg cgacaggtgc ttacaaattc gtggttatgc cagggcgtga cacggttgtg
     4921 gatgtgcagt cgaaaatcta tctgcgcgat aaagtcggca aactgggggt tgcaccgtta
     4981 accagtatgt tcctgtttgg gccgaaccaa ccgtcgcctg caaataacta tcgtccggag
     5041 ttgcacgact ctaacggtct gtctatccat gctggtaatg gcgaatggat ctggcgtccg
     5101 ttgaataacc cgaaacattt agcggtcagc agcttctcca tggaaaaccc gcgaggcttt
     5161 ggtctgttgc aacgaggtcg tgatttctcc cgctttgaag atctcgatga tcgttacgat
     5221 ctccgtccaa gcgcatgggt gactccgaaa ggggagtggg gcaaaggcag cgttgagctg
     5281 gtggaaattc caaccaacga tgaaaccaac gataacatcg tcgcttactg gacgccggat
     5341 cagctgccgg agccgggtaa agagatgaac tttaaataca ccatcacctt cagccgtgat
     5401 gaagacaaac tgcatgcgcc agataacgca tgggtgcaac aaacgcgtcg ttcaacgggg
     5461 gatgtgaagc agtcgaacct gattcgccag cctgacggta ctatcgcctt tgtggtcgat
     5521 tttaccggcg cagagatgaa aaaactgcca gaggataccc cggtcacagc gcaaaccagc
     5581 attggtgata atggtgagat agttgaaagc acggtgcgct ataacccggt taccaaaggc
     5641 tggcgtctgg tgatgcgtgt gaaagtgaaa gatgccaaga aaatcactga aatgcgtgct
     5701 gcgctggtga atgccgatca gacgttgagt gaaacctgga gctaccagtt acctgccaat
     5761 gaataagaca actgagtaca ttgacgcaat gcccatcgcc gcaagcgaga aagcagcatt
     5821 gccgaagact gatatccgcg ccgttcatca ggcgctggat gccgaacacc gcacctgggc
     5881 gcgggaggat gattccccgc aaggctcggt aaaggcgcgt ctggaacaag cctggccaga
     5941 ttcacttgct gatggacagt taattaaaga cgacgaaggg cgcgatcagc ttaaggcgat
     6001 gccagaagca aaacgctcct cgatgtttcc cgacccgtgg cgtaccaacc cggtaggccg
     6061 tttctgggat cgcctgcgtg gacgcgatgt cacgccgcgc tatctggctc gtttgaccaa
     6121 agaagagcag gagagcgagc aaaagtggcg taccgttggt accatccgcc gttacattct
     6181 gttgatcctg acgctcgcgc aaactgttgt cgcgacctgg tatatgaaga ccattcttcc
     6241 ttatcagggt tgggcgctga ttaatcctat ggatatggtt ggtcaggatt tgtgggtttc
     6301 ctttatgcag cttctgcctt atatgctgca aaccggtatc ctgatccttt ttgcggtact
     6361 gttctgttgg gtgtccgccg gattctggac ggcgttgatg ggcttcctgc aactgcttat
     6421 tggtcgcgat aaatacagta tatctgcgtc aacagttggc gatgaaccat taaacccgga
     6481 gcatcgcacg gcgttgatca tgcctatctg taacgaagac gtgaaccgtg tttttgctgg
     6541 cttgcgtgca acgtgggaat cagtaaaagc caccgggaat gccaaacact ttgatgtcta
     6601 cattctgagt gacagttata acccggatat ctgcgtcgca gagcaaaaag cctggatgga
     6661 gcttatcgct gaagtcggtg gcgaaggtca gattttctat cgccgccgcc gtcgccgcgt
     6721 gaagcgtaaa agcggtaata tcgatgactt ctgccgtcgc tggggcagcc agtacagcta
     6781 catggtggtg ctggatgctg actcggtaat gaccggtgat tgtttatgcg gcctggtgcg
     6841 cttgatggaa gccaacccga acgccgggat cattcagtcg tcgccgaaag cgtccggtat
     6901 ggatacgctg tatgcgcgct gtcagcagtt cgcgacccgc gtgtatgggc cactgtttac
     6961 agccggtttg cacttctggc aacttggcga gtcgcactac tggggacata acgcgattat
     7021 ccgcgtgaaa ccatttatcg agcactgcgc actggctccg ctgccgggcg aaggttcctt
     7081 tgccggttca atcctgtcac atgacttcgt agaagcggcg ttgatgcgcc gtgcaggttg
     7141 gggggtctgg attgcttacg atctcccggg ttcttatgaa gaattgccgc ctaacttgct
     7201 tgatgagcta aaacgtgacc gccgctggtg ccacggtaac ctgatgaact tccgtctgtt
     7261 cctggttaag ggtatgcacc cggttcaccg tgcggtgttc ctgacgggcg tgatgtctta
     7321 tctctccgct ccgctgtggt ttatgttcct cgcgctctct actgcattgc aggtagtgca
     7381 tgcgttgacc gaaccgcaat acttcctgca accacggcag ttgttcccgg tgtggccgca
     7441 gtggcgtcct gagctggcga ttgcactttt tgcttcgacc atggtgctgt tgttcctgcc
     7501 gaagttattg agcattttgc ttatctggtg caaaggaacg aaagaatacg gcggcttctg
     7561 gcgcgttaca ttatcgttgc tgctggaagt gctgttttcc gtgctgctgg ctccggtacg
     7621 catgctgttc catacggtct tcgttgtcag cgcgttcctt ggctgggaag tggtgtggaa
     7681 ttcaccgcag cgtgatgatg actccacttc atggggcgaa gcgttcaaac gccacggctc
     7741 acagctgctg ttggggttag tgtgggctgt tgggatggcg tggctggatc tgcgtttcct
     7801 gttctggctg gcaccgattg tcttctcgtt gatcctgtca ccgtttgttt cggtgatttc
     7861 cagccgtgcc accgttggtc tgcgcaccaa acgctggaaa ctgttcctga tcccggaaga
     7921 gtattcaccg ccgcaggtgc tggttgatac cgatcggttc cttgagatga atcgccaacg
     7981 ctcccttgac gatggcttta tgcacgcagt gtttaacccg tcatttaacg ctctggcaac
     8041 cgcaatggcg acagcgcgtc accgcgccag taaggtgctg gaaatcgccc gtgaccgcca
     8101 cgttgaacag gcgcttaacg agacgccaga gaagctgaat cgcgatcgtc gcctggtgct
     8161 gctaagcgat ccggtgacga tggcccgtct gcatttccgc gtctggaatt cccctgaaag
     8221 atattcttca tgggtgagtt attacgaagg gataaagctc aatccactgg cattgcgtaa
     8281 accggatgcg gcttcgcaat aaaaacgtag ttgcctgatg cgctacgctt atcaggccta
     8341 catcgttcct gcaatttatt gattttgcaa gattttgtag gtcggataag gcgttcacgc
     8401 cacatccggc aaaaacaacg agccaataaa aataccggcg ttatgccgta atgccagtca
     8461 gttaagcaac tgactggctc tttttcgggg ctgtggggta tttccagggc ctctccttta
     8521 ccactctc
//
LOCUS       DAZSIO010000160         8492 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZSIO010000160 DAZSIO010000000
VERSION     DAZSIO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.162"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            45..398
                     /locus_tag="ACHX5T_003361"
     CDS             45..398
                     /locus_tag="ACHX5T_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR8468421.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(435..2513)
                     /gene="flhA"
                     /locus_tag="ACHX5T_003362"
     CDS             complement(435..2513)
                     /gene="flhA"
                     /locus_tag="ACHX5T_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFR8468422.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2587..3597)
                     /gene="hypE"
                     /locus_tag="ACHX5T_003363"
     CDS             complement(2587..3597)
                     /gene="hypE"
                     /locus_tag="ACHX5T_003363"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR8468423.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3594..4715)
                     /gene="hypD"
                     /locus_tag="ACHX5T_003364"
     CDS             complement(3594..4715)
                     /gene="hypD"
                     /locus_tag="ACHX5T_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR8468424.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4715..4987)
                     /gene="hypC"
                     /locus_tag="ACHX5T_003365"
     CDS             complement(4715..4987)
                     /gene="hypC"
                     /locus_tag="ACHX5T_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR8468425.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4978..5850)
                     /gene="hypB"
                     /locus_tag="ACHX5T_003366"
     CDS             complement(4978..5850)
                     /gene="hypB"
                     /locus_tag="ACHX5T_003366"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR8468426.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(5854..6204)
                     /gene="hypA"
                     /locus_tag="ACHX5T_003367"
     CDS             complement(5854..6204)
                     /gene="hypA"
                     /locus_tag="ACHX5T_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR8468427.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            6416..6877
                     /gene="hycA"
                     /locus_tag="ACHX5T_003368"
     CDS             6416..6877
                     /gene="hycA"
                     /locus_tag="ACHX5T_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR8468428.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            7002..7613
                     /gene="hycB"
                     /locus_tag="ACHX5T_003369"
     CDS             7002..7613
                     /gene="hycB"
                     /locus_tag="ACHX5T_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR8468429.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            7610..8492
                     /locus_tag="ACHX5T_003370"
                     /pseudo
     CDS             7610..8492
                     /locus_tag="ACHX5T_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
BASE COUNT         1903 a         2306 c         2286 g         1997 t
ORIGIN      
        1 gattaagatg taatataaat gcatcttaat gaggtggagg cagcatgtcc ggcaagcgta
       61 tctctcgtga aaaactgacg attaaaaaaa tgatcgatct ttatcaagcg aaatgcccgc
      121 aggcgtcagc ggagccggag cattacgagg cgttgtttgt ttacgcgcaa aagcggctgg
      181 ataaatgtgt gttcggcgaa gagaaaccag cctgtaagca gtgtccggtg cactgttacc
      241 agcctgcaaa gcgtgaagag atgaaacaga ttatgcgttg ggccgggccg agaatgctgt
      301 ggcgtcatcc gatcttaacc gtgcgtcatc tcatcgatga caagcgtccg gtgccagaac
      361 tgccggaaaa atatcgtccg aaaaaacctc gtgaatagta tgccggacag gcagatctgt
      421 ccggcaattt gcagttaaat caatgccgat ttatcaattc ccagccgctt catccgtgac
      481 agcagggtcg tgcgtttcag ccccagacgt tgcgcagcgc ctttaggccc ggcaaccacg
      541 ctgttggttt ctttcagcac gcgcacaatc aactgatatt catcttcgcc ctcctgggcg
      601 acaaccgttg cggcaggcgg cgtttcaggc tccggtaaag caatatctgg caatgacagc
      661 tgcagcacgt tgccgcgtgt tagcaatact gcgcgctcaa tgacgttttc cagttcgcgt
      721 acgttacccg gccactccat gttgctcaag gtgcgcagcg tctcggcagg aatgctgtcg
      781 atattgcgcc ccagacgacg ggcaattttg aaggtaaagg ctttcgccag cagcggaata
      841 tcttccggac gctcgcgtag tggcggcagg tgaatcggga atacgttcag gcggtaatag
      901 agatcgctac ggaactctcg gtcggcgacc atttttttca gatcgcggtt agtcgcggca
      961 attaaacgta cgtccgtctg aatgattttg ttgctgccga gacgttcaaa ctcctgttcc
     1021 tgcaatacac gcagcaactt cggctgtaac tccagtggca tatcgcccac ttcgtcgagg
     1081 aacagagagc ttttatccgc cagttcaaaa cgaccaatgc gctgggcgtt ggcaccggta
     1141 aaagccccac gctcatgacc aaacagatcg ctttccagca atccggcagg catcgccgcg
     1201 cagttcattt tgaccatgcg gcgattatta cgcccactga gattatggat cgcacgggca
     1261 atcagctctt tacccgagcc agtttcaccg aggatcagca cggtactgtc actttgcgcc
     1321 accatttcaa cttgtttaag cacgctgtac atggcttcgc tgcggccaat aatctcgcca
     1381 aattcactat caacattgtt gagctgctcg gtcagggcga ggttttcatc aaccagccgt
     1441 tctttcagac gatggatttc ctgataggcg agggcgttat cgacagcgat tgccacacgt
     1501 tcggcaatct ggcgcagcaa attcagattg gtagtggtaa acactttctc ttcgcattgc
     1561 gccagtttca gcacgcccag catggtgtcg ccagacatca gcggtaacag gcacaaggtt
     1621 tgaatctggt tgccccaggt gtcgaacaac atgcgttcat agggggctaa atcgtcccgc
     1681 tcgtagagat tgatcagcag catctcttta cttttgaaca cgcgttcggt gagggttccg
     1741 gcttcatcga cttcgctctg ttcgtgggcg ggatgttgtt tatcaagata gtgagtggag
     1801 tagatgttga gtttgttttt acggtggctg cgtaagacaa tgctgatatc gtcaatgtcg
     1861 aaatagtaat ggatttcttt ggcgacttcg ctgaccagtt cgtccatatc caggcgggaa
     1921 agcaccgcgt tggtaatggc aaccaggatg cggaagttat cgcgctcccg gcataacaac
     1981 tcatagtcga cattgttgac gacgcggctc tggatttgtt cggtgacgac agaaacgatc
     2041 tgcgtaaatg tttgcagacg attgaactct ttttcgctcc agggacgctc gtcataacga
     2101 ataaattcac agccaccaaa aatatgccct cccgccgcca gtggcatcag gcaatagtga
     2161 ccaaatttgg ggtatagccc acctgcggcc agctgcggcc aggtttcaca aaattcttcg
     2221 taactgcaat gcagcgtatc agggcgcgac aaaatgctgc gtaccggacc gtgtgccaga
     2281 acagtttcgt cttcatattt aatgggggtg tctttttcac gcgacgcgta ataagacgca
     2341 cgttgagtct gcgcttgcca caacacaata gccgcgttgt cggcgagcgc agaacgcttt
     2401 accagttgcg aaagagcctc acacagcgag gccagatcgg gctgctgcaa tagtgtccga
     2461 gtgatgtcga acaacccttg ctgtccgaga tcactcatcg gtgtatatga cattttgttc
     2521 ttccaggaaa gcaccgcaaa gcggtgcaaa aggtcattga ctaactatag gagatttaga
     2581 attttattag catatacgcg gaagcggttc ggcgtgtggt aaatcgaggg ttcgtttcac
     2641 gccatacaga ccggcaagac gaacaccttt acgttccacc acttcaccaa tcagcgccgc
     2701 gtctttcccc agtggatggg aatgtaacgc tgccagcact tgctctgccg cgttgcgttc
     2761 aacggcgatc accagtttgc cttcgttggc aaagttcagg gcgtccagtc ccagcaattc
     2821 gcaaacgccg cgcacggcag gtttaaccgg cagcgctgat tcagaaattt caataccaca
     2881 accgcaggct gccgcgaact catgaaccac cgcgtttaca ccaccacggg tggcatcacg
     2941 cagcgctttc acgccgggaa tgtcacgcag cgtctgaata agcggcgtca gcaccgcgca
     3001 gtcgctgacc agttcgccat ccagccccag ctgctcacgc aggttaagga tagtcgcccc
     3061 gtggtcgccg agtgtaccgc taaccagcaa tacatcgcct gcggttagcg tctgtgcgcc
     3121 ccagtgaata ttcgccggaa ttgcgcccat gccagcggtg ttgataaaca gtttatctgc
     3181 cgcgccgcgc tgcaccactt tagtatcgcc agtaacgatg gcaatgcctg ccgtgcgggc
     3241 ggtttctgcc atgctggtca ctacggcttt cagtgtctcc atcggcaatc cttcttcgag
     3301 gataaagcca caggagagat agcgcggaat agcgccactg cccgcaacgt cattcgcggt
     3361 gccgcaaatc gccagcttgc cgatattacc gccaggaaag aacagcgggt caatgacgta
     3421 actgtcggtg gagaacgcca gacggtcgcc ttccgctacc agctgcgcca gatcaagacg
     3481 tgcctgatct tcctgctctg ccagccacgg gttggcaaag gcttccataa acaggctgtt
     3541 gattaattgc tgcatcgcct ggccgccgct gccgtgggcg agttggatat tattcacgct
     3601 tcactctcct gctgacgata ctgataccac gcggcgcacg ctccttcgga ggagaccatc
     3661 agtgcaccaa atgcggtttg aggattacag gtgttaccaa acagcgggca ttgatgcggc
     3721 ttacatttgc ccgttaatac ctcaccacaa cgcgcgcgcg ggtcatcgca gacctgctgc
     3781 ggtgccgggc ggaaatgtgc ttcggcatcg aatcgttgat aatccggcgt caggtgcacg
     3841 ccagaagatt caatcacgcc taagccgcgc cattcgctgt cgccgttgac acagaacaca
     3901 tcggcaatcg cctgttgcgc cagtaggtta ccggcatccg gtaccacccg acgatactga
     3961 ttctctacct tgctgtgggc cgctattttc tgctccacca gcatgaccac gccttgcagt
     4021 agatcaaggg gttcgaaacc agcgaccacc agcggacgat gaaaatcgct ggcgataaaa
     4081 ttataggcat cagtgccgat aaccatacta acgtggcccg gcgcgaggaa cgcgtcgata
     4141 ccgttatccg gctgttccag caaactgcgc agcgtcggga taagcgtaat gtgctggcag
     4201 aagaagtaaa aattctgcac atcacgcgct ttcgcctgtt gcagagtgat ggcggtggtc
     4261 ggcatggtgg tttcaaaacc taagccgaag aacaccactt tgcgggttgg attctcctgc
     4321 gccagtttca acgcatccat cggcgagtaa acgatgcgca catcggcacc gcgtgctttt
     4381 gcctgcaaca gcgatccctg tttccctggt acgcgcatgg cgtcgccaaa ggtacagaag
     4441 atgacttccg gatggctggc aatctccacg caggtgtcga ttctacccat cggcagtacg
     4501 cacaccgggc aacccggacc gtggataaac tcaacgtttt caggcagtaa ctggtcgagg
     4561 ccgaatttaa aaatggcgtg ggtatgaccg ccacacactt ccataatccg cagagggcgt
     4621 tcggcggtgt aagagagatg tgaagcacgt tcgcgcagat gctcaattaa ctgcatcacc
     4681 tgttccggcg cgcgatattc atcaacaaaa cgcattattt ttcctcgcca tacaacagcg
     4741 cgccgacatc cggctcaacg tcaaacatgt tttgtaaggc gtcgagagtg tcgcgtgctt
     4801 cggcttcatt aattacgctc atggcaaagc caacgtgaac cagtacccac tggcccacgc
     4861 gcggctgacc gttttcatcg cagctgccga ctaacgttaa atcgacatcg cgctgaatgc
     4921 cgcagacgtc gactttcgcc tggttgccgt caatggtgcg gatctggccg ggaacgccta
     4981 tgcacatcgc tgtgtctcca gccagttcag ccactggtcc atcccttcgc cgctggtggc
     5041 ggaaataagg atgatttcaa tttctggatt gacttcgcgg gcgcaggcga tgcacttctc
     5101 aacgtcaaag ttgagatacg gcaacaggtc aactttgttg agcagcatca gcgaggcggc
     5161 ggcaaacata tgcggatatt tcagcggttt gtcttcacct tcggtaacgg aaagcaccgc
     5221 tactttgtgt ttttcaccga gatcgaagct ggccgggcat acgaggttgc caacgttttc
     5281 gataaacaga ataccgttat cgtccagtgg cagacgcggt gcggcgtcgg caatcatctg
     5341 tgcgtcaaga tggcagcctt taccggtgtt cacctgaatc gctggtgtac cggtagcgcg
     5401 aatgcgtgcg gcatcgttca cggtttgctg gtcgccttca ataaccgcgc acggaacgct
     5461 gtctttcagg cgcattaagg tttccgtcag cagggtggtt ttaccggaac cagggctgga
     5521 aaccaggttg agcaccagtt gcttgcgggc agcaaagcgc gcgcggttgc gttcagccag
     5581 acggttgttt ttgtccagca cgtcaatttc gacttccagc atccgacgct ggctcatgcc
     5641 cggtgtgtga gtgcccgctt cgccatgacc gtaatgcagg tcgccttctt cagtctggct
     5701 gggggtaaat tcaggcgctt taatgccggt gattttcatc tgcgggcgtg ccgccggggc
     5761 aaatggcgcg ctacgaaacg cggaatgagg gttatgttca tcaccctcga tatacaggtt
     5821 gccttcaccg caaccgcatg ttgtacacat cgctcactcc tggtctattt ctatccgccg
     5881 aatctgtaaa ccgtcgtctg ccacaatctg tagcatgtca ccatgacact gtggacagcg
     5941 gcggacgcgc tgggtcagta gcgtcacata ctgttggcat gtttcacacc agcattcggc
     6001 ctcttgttct tcgaggtgca gtttacaacc ttccgccacg ctgccgcggc aaaccagatc
     6061 aaaacaaaag gcaagagagc tggtttcgac acaagaaaat gcgccaattt tgagccagac
     6121 cccagttacg cgttttgcgc cgtgttttgc ggcctgctgt tcgatcaatt ccagtgcccg
     6181 ttggcagagg gttatttcgt gcatatcgcc tcccattaac tattgccagc tacaagcaat
     6241 aattgcgcca gtgttgatta tccctgcggt gaataatgtc gatgatgtcg aaatgacacg
     6301 tcgacacggc gacgaaattc atctttagct taaaaatctc tttaataaca ataaattaaa
     6361 agttggcaca aaaaatgctt aaagctggca tctctgttaa acgagtaacc tgacaatgac
     6421 tatttgggaa ataagcgaga aagccgatta catcgcacag cggcatcgtc gcctacagga
     6481 ccagtggcac atctactgca attcgctggt tcaggggatc acgttatcga aagcgcgcct
     6541 gcatcacgcc atgagctgcg cgccggacaa agaactctgt ttcgtccttt ttgaacattt
     6601 tcgtatttac gtcaccctgg cggatggctt taacagccac accatcgagt attacgtcga
     6661 aacaaaagat ggcgaagaca aacagcggat tgcgcaggcg caactgagca ttgacggcat
     6721 gattgatggc aaggtcaaca tccgcgatcg cgaacaggtt ctggaacact atctcgaaaa
     6781 aatcgctggc gtttacgaca gcttatacac cgccattgaa aacaatgtgc cggtgaattt
     6841 aagccaactg gtaaagggac aaagcccggc agcatgagct gaggctttgc ccgttttgca
     6901 ggcgttacgc ctgtttgggg atgggcgtgt cgatgagtgt cgaaaatgac atttcatcgg
     6961 catgttttcg tcaaaaatga caatcacctg aggaatgcct ggtgaatcgt tttgtaattg
     7021 ccgactccac gctctgtatc ggctgccaca cctgtgaggc cgcctgttca gagacgcatc
     7081 gccagcacgg cctgcaatca atgccgcgcc tgagagtgat gctcaatgaa aaagaatctg
     7141 cgccgcagct ctgtcaccac tgtgaagatg caccctgcgc ggtggtctgc ccggttaacg
     7201 ccatcacccg cgttgatggg gccgtgcagt tgaatgaaag tctgtgcgta agctgcaaac
     7261 tgtgcggcat cgcctgcccg tttggcgcaa ttgaattttc cggcagccgt ccgctggata
     7321 ttccggcaaa cgccaatacc ccgaaagcgc caccggcacc gcctgctccg gcgcgtgtca
     7381 gcacattgct tgactgggtg ccaggtattc gcgcgatcgc cgtcaaatgt gacctttgta
     7441 gctttgatga acaaggtccg gcctgtgtgc ggatgtgccc gactaaagcc ctgcatctgg
     7501 tggataacac cgatatcgcc cgcgtcagca aacgtaagcg tgagctgacc tttaacacgg
     7561 actttggcga tctcaccttg tttcagcagg ctcaaagtgg agaggctaaa tgagcgcaat
     7621 ttccctgatc aatagcggcg tggcatggtt tgtcgccgcc gctgttctgg catttctctt
     7681 ttcaaaaagc gctaagtggc tggatagccg gaattggcgg cgcggttggt agtctgtata
     7741 cggcagccgc gggcttcact gtactgactg gcgcggttgg cgtgagcggt gcgctgtcgc
     7801 tggtaagcta cgatgtgcaa atctccccgc ttaacgcgat ttggctgatt accctcggcc
     7861 tttgcggtct gtttgtcagc ctctataaca ttgactggca tcgccacgcg caggtgaagt
     7921 gcaacggctt gcagatcaat atgttgatgg ctgccgccgt ctgcgccgtc atcgccagca
     7981 acctcggcat gttcgtggta atggccgaag tcatggcctt gtgtgcggtg ttcctcacca
     8041 gcaacagcaa agagggcaaa ctgtggtttg cgctgaggcg tcttggcact ctgctgctgg
     8101 tgattgcttg ctggctgctg tggcagcgtt atggcacgct ggatctgcgc ctgctggata
     8161 tgcgtatgca acagctgccg ctcggttccg atatctggct gctcggtgtg attggctttg
     8221 gcctgctggc tgggattatt ccgctgcacg gctgggtgcc gcaggcacat gctaacgcct
     8281 ctgcgccagc tgccgcgttg ttttctacgg tagtcatgaa aattggcctg ctgggcatgt
     8341 taaccctgtc actgctgggc ggtaatgcac cgctgtggtg ggggatcgcg ctgctggtgc
     8401 tcggcatgat caccgcgttc gtcggtggtc tgtatgcgtt gatggaacac aacatccagc
     8461 gcctgctggc ttaccacacc ctggaaaata tc
//
LOCUS       DAZSIO010000161         8394 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZSIO010000161 DAZSIO010000000
VERSION     DAZSIO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.163"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..1213
                     /locus_tag="ACHX5T_003371"
     CDS             <1..1213
                     /locus_tag="ACHX5T_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR8468430.1"
                     /translation="TKKTKSDNYSKHHKNTLIDNKALSLFKIDDHEKVIGLIQKMKRI
                     YDSLPSGKITKETDRKIHKYFIDIALYANNKCDDRITRRVYLSKEKDVSIKVVYFINN
                     VAVHNNTIEIPQIVNGGYDFSHLSLKGIVIKDEDLSNSNFAGCRLQNAIFQDCNMYKT
                     NFYYAIMEKILFDNCILDDSNFAQIKMADGTLNACSAMHVQFYNAAMNRANIKNTFLD
                     YSNFYMAYMAEVNLYKVIAPYVNLFKADLSFSKLDLINFEHADLSRVNLNKAILQNIN
                     LIDSKLFCTWLTNTFLEMVICTGSNMANVNFNNANLSNCHFNCSILTKACMFNTRLYR
                     VNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDNINAVAM
                     KITADIMQHAD"
     gene            complement(1367..3016)
                     /locus_tag="ACHX5T_003372"
     CDS             complement(1367..3016)
                     /locus_tag="ACHX5T_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR8468431.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(3167..4516)
                     /gene="ghxP"
                     /locus_tag="ACHX5T_003373"
                     /gene_synonym="yjcD"
     CDS             complement(3167..4516)
                     /gene="ghxP"
                     /locus_tag="ACHX5T_003373"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR8468432.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(5222..5686)
                     /gene="soxR"
                     /locus_tag="ACHX5T_003374"
     CDS             complement(5222..5686)
                     /gene="soxR"
                     /locus_tag="ACHX5T_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR8468433.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5772..6095
                     /gene="soxS"
                     /locus_tag="ACHX5T_003375"
     CDS             5772..6095
                     /gene="soxS"
                     /locus_tag="ACHX5T_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR8468434.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(6098..7675)
                     /gene="pdeC"
                     /locus_tag="ACHX5T_003376"
                     /pseudo
     CDS             complement(6098..7675)
                     /gene="pdeC"
                     /locus_tag="ACHX5T_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            8104..8385
                     /locus_tag="ACHX5T_003377"
     CDS             8104..8385
                     /locus_tag="ACHX5T_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFR8468435.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         2381 a         2119 c         1898 g         1996 t
ORIGIN      
        1 tacaaagaaa acaaaatcag ataattacag caaacatcat aaaaacacgt taattgacaa
       61 taaagccctc tctcttttca aaatagatga tcatgaaaaa gtgataggct tgattcagaa
      121 aatgaaaaga atttatgata gtttaccatc aggaaaaatc acgaaagaaa cggacaggaa
      181 aatacataaa tattttatag atatagcgtt atatgcaaat aataaatgtg acgatagaat
      241 tacgagaaga gtttacctta gtaaagaaaa ggatgtatcc attaaggtgg tatattttat
      301 aaataatgtc gccgtccata ataatactat cgaaattcca cagatagtaa atggtggtta
      361 cgatttttca caccttagcc tgaagggtat cgtgattaaa gatgaagatt tatccaattc
      421 gaattttgca ggttgcagac tacaaaacgc tattttccag gactgtaata tgtataaaac
      481 gaatttttat tacgccataa tggaaaaaat actttttgat aattgtattc tcgatgactc
      541 aaatttcgct cagataaaaa tggccgacgg aactctaaat gcatgctccg ctatgcatgt
      601 tcaattctac aatgcagcaa tgaatagagc caatattaaa aataccttcc ttgactattc
      661 aaatttttat atggcgtaca tggctgaggt aaatctttat aaagtaatag cgccatatgt
      721 taatttattt aaagccgacc ttagtttctc taaactcgat ttaattaact ttgaacatgc
      781 tgatctgtct cgcgtcaatc tgaacaaagc aatcctccag aatataaact taattgatag
      841 caaactcttt tgtacgtggc tgacaaatac attcctcgaa atggttatat gtaccggctc
      901 taatatggct aatgttaatt ttaataatgc caatttaagc aactgccatt tcaactgttc
      961 tattttaaca aaagcctgta tgtttaatac ccgtctctat cgggttaatt tcgatgaggc
     1021 tagcgtccag ggaatgggca tttccattct ccgtggggag gaaaatatcc ccattgatag
     1081 tgataccctg gtaacactac agaaattctt tgaagaagat tgtacctctc atactggcat
     1141 gtcacaaact gaggataata ttaatgcagt cgctatgaag attactgcag atattatgca
     1201 acacgcagat tgaggttaag tcgaagaaca gccaatgaat tgactttaca agagagataa
     1261 cttatttgtt ttagaatcat gaatgttcgc ccgagctaac ctgttaaagg tgcaaaagat
     1321 tgcacctttt tcagattacc tcgggcctgg ttacgtcgct ttcggcttac tgattttcct
     1381 caattagcaa cgcttcaagc aaatcgagat cgtgcagtaa tttttgcagc gtttcgttgc
     1441 tgatctcccg cgtggcgcgc aggtggtaca gttcagcacg ttcagaacgc aatgccgcca
     1501 gacggaagcg acgctcaagg ttctcttcct gcacggaact ttcaacgtcg ttacgtccat
     1561 cggcgcgacg acgcaggtta ccaatgacgc gagaactgac ctccgtaagc aactggttat
     1621 cgatattctc ttcggtatcc gccgccagac gctcctccat tttctggatc gccacaatcg
     1681 ccacttctgc cgttgccgct cgcgcaatac gttcttcttt caattgttgc gaatggtccg
     1741 cgacttcaat gtgttgtagc agaataggca acatcaccac gccgacaaac agcgagaaga
     1801 gaatgacgcc agccgccagg aacaccagct catagcgcgc cgggaagacg ttaccatccg
     1861 gcaagagcag cgggattgag agcacaccgg ccagagtgat cgccccacgc accccagcga
     1921 aagacgcgat caggatttct cgtgtggtcc acgaaccaaa ctccatcggc ttcttcttca
     1981 ggaagcggtt gctgaacttt ttcatcgtcc acagccagcc gaaacggacc agcatcagcg
     2041 ccgcatatat cagaataata tcggtaaaca gcatccagat ttcgacgtta gggtcgattt
     2101 ctgccgccat cagcgacgtc tccagaatac ccggcagttg cagacctaac agcaggaaca
     2161 ccatgccgtt aaagacaaat tccagcatcg cccaggtgct gtttgcgcgc aggcgcattg
     2221 ccagtggcgc acggcgcatc acaccggagc gggtgatggt catcccggcg gcaaccgcag
     2281 cgaggatgcc cgaaacgcca atatgttcgg caatcagata agaagcgaat ggcagcaaga
     2341 acagcagaac gatctgcgtc gcgggttcat caccgcccca gcggctgagg aatcgcagcg
     2401 agcgaccata cagccagctc accacaaaac cggcaagaat accgccaatg gctactttca
     2461 taaattcgac cgtcgcaccg ccgacagtga agatcatcgt ccccatcgcc actgccacgg
     2521 caaacttcaa cgacaccagg ccggaagcgt cattcatcaa cgcttcgccc tgcaaaatgc
     2581 ccatgatttt tttcgggatg cgcccttcgc caacaatccc ggagagcgcc acagcatcgg
     2641 tcggagaaag taccgccgcc agcgcaaagg cggggatcag cggaatgccc ggcaccaccc
     2701 agtaaataag gaagccaatg ccgaccacgg tgaccaccac cagcgccagc gcgaggccga
     2761 aaatctctcg accatgttcg agaaattcac gggtcggcgt tttccagcca tcagcgaaca
     2821 gcaacggcgg gataaacaag actaaaaaga gttcaggatc aaactccaca tgcaaaccaa
     2881 acgtcggcca cgccagtagc gcaccgatgg cgatttgcat aagcggaagc gggatctgaa
     2941 agggcatgac acgagtgacc accccggaca gcgagaccac gagggtcatt atcagtatgg
     3001 tgaagaagat ttccatgcgt tccctgtttg cgatgttttt tatgatctga gaaagacgtt
     3061 atgcctgaat tctcattttt tcagaatatc gcaaaagctc acacaatgtg cggagaaaat
     3121 aaaaacgggc agccattgcc acccgttttc agaatccgat gaaagattag atagcccagc
     3181 cacccgcata gaaggtcacc agcgccacgg cgataacgac cgtaccgatg ttcaacttgc
     3241 gccattcacc ggaaaccaga cgaccaatca ccagagtcgc gaagccgatc atgatgcctg
     3301 ttacgatgtt acaggtcagc acgatgaata ccgccgtaac cagacccgcc atcgcatcaa
     3361 caaaatcagc aaagtcgatt ttcgccacgt tgctcagcat cagcaggcca acgtacatca
     3421 gcgccggagc cgttgcatac cccggaacga ggtaagaaag cggagagagg aacagaatca
     3481 gcaggaacag cacgccaacg gtgatagccg tcaacccggt tttaccgccc gccgccgtac
     3541 ccgccgcaga ctcgatgtat accgctgccg gagctgcacc caccaggcca gagaaaacgc
     3601 tgctcatgga gtcagtggtc agtgctttcc cgccgtcgat gatctgccca tctttatcca
     3661 gcaggttcgc ctggccggcg acagcgcgga tagttccggt ggcatcaaat actgccgtca
     3721 tcaccagcgc cagaacgctt ggcaggacta caggattcag cgcgcccata atgtccaggc
     3781 tgccaatcag tgaattgccg ttttcatcgc tcaatgaagg catggcgaaa acgccggaga
     3841 aatggacgtt aggatcgaag atcaaaccga caattgagat accgataatg gtcagcagaa
     3901 tgccaccagg gactttcagt ttttccaagc cgatgatcac cgccagacct gccagtgaca
     3961 taatcaccgg gaaggtcgcg aaatcaccca gcgcaacggg cagaccatca agcgggtttt
     4021 taatcaccag accgacacca ttagcggcaa tgagcagcag gaacagaccg ataccgatcc
     4081 ccgtgccgtg cgccacaccg tgcggcaagt tgcgcaaaat ccagctacgg atacccgtgg
     4141 cagaaattac cgtaaacagc acacccatca ggaacacggc acccagcgcg acaggtacgc
     4201 taatatgttg ccccagcacc aggctgaatg cggtaaacgc cgtcagggag atggcgcaac
     4261 caatcgccaa cggcagatta gcccacagac ccatcacgat agaaccgagt ccggcaacca
     4321 gacaggttgc aacgaaaact gccgcaggcg ggaagcctgc tttacccaac atacctggaa
     4381 cgacgatgac cgagtagacc atcgccagaa acgttgttaa cccggcaacc acttcctgac
     4441 ggacagtgct tccacgttgt gaaattttaa accaggcgtc gagtgaaccg ccggtacgcg
     4501 ctgatggcgt agacataaga aaacatcccc tgagagttta attttcgtca agatgcgtgg
     4561 cggacaatcc cctgccagcg aaacgttatc ttcctgctcc aggtttgcgg caatttggtg
     4621 cgtcgcaaaa aagcaaacgt ttagctccgc gcatacaaaa cgggtggtaa aaaaaagcaa
     4681 acgattatcc ggcgatcata gcggcttttt caaccgaagt ttgctgcttt tttctctttt
     4741 attgtcttga cgatgaaaaa ccaggcaatt gatgcgcaaa cgttatcgtc gatgtgcaag
     4801 attgcagcgt tatcgagccg tataccgttc tggattgcga taaaaaatcg tctgagatga
     4861 caaaaatatt gcggctaaaa agtatacttt tacactggcg aagaaacgtc ggtgacatac
     4921 aattaaagca tcaacaccaa ccggaacctc caccacgtgc tcgaatgagg tgtgttgacg
     4981 tcgggggaaa ccctcctgtg ttccagcggg atagagagaa agacaaagac cggaaaacaa
     5041 actaaagcgc ccttgtggcg ctttagtttt gtgtttgcgg ataattccaa aagagctcga
     5101 tcagtcccct cgccccttcg gggagagggt tagggtgagg ggaacaggca gcaccagtgc
     5161 aaatatttac cctcaccccg gccccatagt taacgatgag tcagcttgtc cttgtggcgc
     5221 tttagttttg ttcatcttcc agcaagcgtg cgccggtacc ttcttctcct aagcggtcgc
     5281 ccgggttacg caacgggcaa tcactgcgcg aaaggcagcc acaaccaata catccgtcca
     5341 gttcgtcacg cagcgccact aaggtatgaa tgcgccgatc caactcttct cgccattggg
     5401 acgaaagctg tttccactct ttcgcactta acgtatgccc ttctggcaac acgccaaacg
     5461 cttcaccaat ggtcgccagc ggaatgccaa tacgctgagc gattttgata attgcgacat
     5521 atcgcaacac atcacgttta tatcgccgct gattgccgct gttacggata ctagtaatca
     5581 accctttgct ttcatagaaa tgcagcgccg ataccgccac accgctgcgt ttcgccactt
     5641 cgccaggggt tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct
     5701 caagttaact tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa
     5761 gaggcagatt tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgtgc
     5821 atattgacca gccgcttaac attgatgtcg tcgcaaaaaa atcaggctat tcaaagtggt
     5881 acttgcaacg gatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac
     5941 gccgcctgtt actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg
     6001 caatggacct gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg
     6061 atcgcactcc cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc
     6121 gcgcgggtaa ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa
     6181 accatccctg gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt
     6241 cgacgccttc agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg
     6301 caatcaaatg actggttttg tgggtggtca gcgtttcaac aaacgattta tcgattttca
     6361 aaatatcgac attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat
     6421 caattgccac ttcgtaacca gcctggcgga aagccagaat taatcggcgt cattttttcg
     6481 acatcaagaa atgcatgctc agtcacttca aatttaattt gctgcggacg caccgcgtat
     6541 tgctctgttt tctgattgat tcgcgctatc aaccgtgacg tatgaaaatc ggaggccgac
     6601 aggttaatag aaacatagcg atcggcatgt gttgccaggt acgcgcccag atcgcggaag
     6661 acattatcaa taacataatc agttatctgt tctatcatcc cctccttttc tgccagcgga
     6721 ataaactctg ccggattcat tatttgcccc tgctcaccag gccaacgtaa caacgcttca
     6781 gcgccgatac atttttctgt tttgatatcg attattggct ggtaataaag acaaagttga
     6841 tgtttttcga gggcgcgttg caatttacgt ttgggagata aatagttttg tcgaatacgt
     6901 agccagagta atagcagaac aagactcccc aaaataccgg cgggcaacgc aaaaataaga
     6961 tgattataaa aatgggttat aagacgttga tatgaagtcg caacaatggc tgcaattggg
     7021 cgttttgtcg aataaattgt cgcatataaa tagccatttc tttgtacggt taaatccttc
     7081 aaatgaatca gcggagaaaa cgttgctgcc gaggcctctt tgcttaacga gaaaaaggtt
     7141 ttcatcaccg tatcatacac accccattgc aatgtcgggt catcagacat cacttcactc
     7201 cagaagagag ggttgataac cgccacataa tttccccgct gcatataggt cattttatag
     7261 ccagagaaaa aaggcgtatc gcggtaataa tagatagaaa cgttaggttc acgcttataa
     7321 tcggccggtg caatcgtata gccgtttaca ggcgctatca gcgatgagca taaaaaatgg
     7381 ttatcacgaa ttcattaata taaagatagc cacgaataat attcaacatt cgcttttgat
     7441 gggctggagt gcaaacttgc ccctgatagc gttcagctgc atcgctcact aaatctgcct
     7501 ggcgaatgac cagctcggat ttatccagtg ccagttgagc aaaggttcgg agctgattat
     7561 tcacttctga ttttgcccaa aggaaggcaa tccatagcga aagaatgatg ggaaagagaa
     7621 ctaaaaagat aatgcccggc aacgccagta attggtgtcg tgcacgatga ctcattgcta
     7681 tcaatttcct gcctgtttat acccggccat tgcaaggtga aaccctggct accgacgtct
     7741 ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga taccttttta
     7801 atcgtcacct ggatgaaaat aattcattga tattcaaaat taattttcta caggaataca
     7861 gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataattt gtacagcaat
     7921 aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagttc acaaaattaa
     7981 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac
     8041 cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca gaggaattgt
     8101 ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcaacttct tagtgccgta
     8161 atgatgtttc tggccagcac actcaacatc cgttttcgtc ggtctgatta tgtcgggctt
     8221 gcggtgatca gcagcggtct gggcgttgtt tccgcctgtt ggttcgcaat ggggttgctt
     8281 ggcatcacaa tggcggacat caccgctatc tggcacaaca tcgagtcggt gatgatagaa
     8341 gagatgaatc agacaccgcc acaatggcca atgattttga cctgatacgt agaa
//
LOCUS       DAZSIO010000162         8332 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZSIO010000162 DAZSIO010000000
VERSION     DAZSIO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.164"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(168..566)
                     /locus_tag="ACHX5T_003378"
     CDS             complement(168..566)
                     /locus_tag="ACHX5T_003378"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFR8468436.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(566..793)
                     /gene="vapB"
                     /locus_tag="ACHX5T_003379"
     CDS             complement(566..793)
                     /gene="vapB"
                     /locus_tag="ACHX5T_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFR8468437.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            875..6128
                     /gene="traI"
                     /locus_tag="ACHX5T_003380"
                     /pseudo
     CDS             875..6128
                     /gene="traI"
                     /locus_tag="ACHX5T_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6148..6894
                     /gene="traX"
                     /locus_tag="ACHX5T_003381"
     CDS             6148..6894
                     /gene="traX"
                     /locus_tag="ACHX5T_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFR8468438.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6949..7509
                     /locus_tag="ACHX5T_003382"
     CDS             6949..7509
                     /locus_tag="ACHX5T_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR8468439.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7640..7852
                     /locus_tag="ACHX5T_003383"
     CDS             7640..7852
                     /locus_tag="ACHX5T_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR8468440.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8096..>8332
                     /locus_tag="ACHX5T_003384"
     CDS             8096..>8332
                     /locus_tag="ACHX5T_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR8468441.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1913 a         2086 c         2670 g         1663 t
ORIGIN      
        1 atcagtgaat ccggtgaagt ggtggatatg gccgcttatg aggcatggca gcaggaaaat
       61 catccggaca cctggcagca gatgcagcgt cgtgaagagg tgaacatcaa tgtgcaccgg
      121 gaacgcgggg aggatgttga gccgggagat gatttctgat gaacaggtca gctccagtct
      181 tcagttctca ggccgcccac acgttcaaat tcccgggtgt tattagtcac aataatcagc
      241 ccccgactgc gggcatgacc tgcgatcatt tgatcaaatg gcccgacagg gcgtccctga
      301 cgggcaagtt ctgctcttat ctggccggtg tgtgtggctg cagcagcgtc gtaatccaga
      361 acatcaatgc gggaaacaaa tccctcgatc acagcgagat tacgttcagg catctggctt
      421 ttttctgcac catatatcag ctccatcagg gtgaccgaac tgatacacat tttcccctgg
      481 ttcaggttaa aacgctccct gacgctggcg ggtttgttct ttatcgtaaa aatgcagatg
      541 ttggtatcga gcataaactt cagcatcaga atgactccct ttcttgcatg ccgggctgtt
      601 ccctgttatc cataaaatcg gtgctgacgc tgtgtccgtc gaaccattcg tcccatgtct
      661 ctccggctgg cgtaatgatt ctggttcgtc cgacagcaat cacttcaacg cgttttacgt
      721 tttccggcaa tgcaaccgct tttggcagtc tgaccgcctg gctgcggttg ctgagaaata
      781 cggtggtttc cattatttgc ctccttatgt ggatatgtct tgtgtatatc tattgtggga
      841 ttgataccgg gatatgtcaa agggatatac gtttatgatg agtatcgcgc aggtcagatc
      901 ggccggaagt gccggtaatt attataccga caaggataat tactatgtgc tgggcagcat
      961 gggagaacgc tgggccggcc agggggctga acaactgggg ctgcagggca gtgtcgataa
     1021 ggatgtcttt acccgccttc tggagggcag gctgccggac ggggcggatt taagccgcat
     1081 gcaggatggc agtaacaggc atcgtcccgg ctacgacctg accttctccg ctcccaaaag
     1141 tatctccatg atggccatgc tcggggggga caaacgtctt attgaagcac ataaccaggc
     1201 cgtggatttt gctgtccgtc aggtggaggc cagtgcctcc acctgacgga cggacagtca
     1261 gaaacggtgc tgaccggtaa tctggtgatg gcactgttta accacgacac cagtcgcgat
     1321 caggaaccac agttacacac gcatgcggtg gtgactaatg ttacgcagta taacggcgaa
     1381 tggaagacac tgagcagtga caaagtgggg aaaacggggt tcattgagaa tgtgtacgct
     1441 aatcagattg cctttggcag gctctaccgg gaaaaactga aagagcaggt tgaggcgctg
     1501 ggctatgaaa ctgaagtggt cggtaagcac ggtatgtggg aaatgccggg tgtaccggtg
     1561 gaggcctttt ccggacgctc acagactatc cgggaggccg tcggggaaga cgcctcgctg
     1621 aaatcccggg atgtggcggc gctggatacg cgtaaatcca aacagcacgt cgatcctgaa
     1681 gtcagaatgg ccgagtggat gcagacgctg aaggaaaccg ggttcgacat ccgggcatat
     1741 cgtgacgcag cggaacaacg ggcatatacc cgcacgcaga cacccggacc cgcttcacag
     1801 gacgggccgg atgtgcagca ggcggtgaca caggccattg ccggattaag tgaacgcaaa
     1861 gtgcagttta tgtatacgga cctgctggcc aggacggtcg gcatactgcc gccggaaaat
     1921 ggtgtgattg aacgggcacg cgccggtatc gatgaggcca tcagccgtga gcagcttatc
     1981 cccctcgatc gtgagaaggg gctgttcacg ttcgggattc atatgctcga tgagctgtca
     2041 gtccgggcac tcagtcgtga catcatgaaa cagaaccggg tgaccgtaca tccggagaaa
     2101 agtgtccccc ggacggccgg ttacagcgat gccgtcagtg ttctggcaca ggaccgtccg
     2161 tcgctggcca ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg ggtggctgaa
     2221 ctggtcatga tggcccggga gcaggggcgg gaggtgcaga ttatcgctgc tgaccgtcgc
     2281 tcgcagatga acctgaagca ggatgaacgg ctgtccggtg aactgataac cggacgtcgt
     2341 cagttgctgg aaggaatggc cttcacgccg ggcagtaccg ttatcgtcga ccagggcgaa
     2401 aaactctccc tgaaagagac gttaaccctg ctggatggtg ccgcacgtca taacgtacag
     2461 gtcctgataa ccgacagcgg gcagcgaacc ggtacaggca gtgcactgat ggccatgaag
     2521 gatgccgggg tgaacacata tcgctggcag gggggagaac agcgaccggc caccatcatc
     2581 agtgaaccgg accgtaatgt cggctggccg gagattttgc ggccagcgtg aaagccggag
     2641 aagagagcgt ggcacaggtc agcggagtac gggaacaggc catactgaca caggccattc
     2701 gcagtgagct gaaaacacag ggcgtgctcg gacacccgga ggtaaccatg accgccctgt
     2761 caccggtctg gctggacagc cggagccgtt atctgcggga tatgtaccgc ccgggaatgg
     2821 tgatggagca gtggaacccg gagacacgca gtcatgaccg ctatgtgaca gaacgtgtga
     2881 cggcgcagag tcacagcctg accctgcgga atgcgcaggg cgaaacgcag gtggtgcgta
     2941 tttcttccct ggacagcagc tggtcgctgt tccggccgga aaaaatgccg gtggcagacg
     3001 gtgagcgtct gagggtgaca gggaaaattc ccggactccg cgtctccggc ggtgaccgcc
     3061 tgcaggtggc atccgtcagt gaagatgcga tgacggttgt tgtgccggga cgggctgaac
     3121 cggccaccct gcctgtgagc gattcaccgt tcactgcact gaagctggag aacggctggg
     3181 tggaaacgcc cgggcattcc gtcagcgaca gtgcgacggt ttttgcctcc gtcacacaga
     3241 tggcaatgga caatgccacc ctgaatggtc tggcccgcag tggtcgtgat gtccggctgt
     3301 attcctcact ggatgaaacc cgtactgcgg aaaaacttgc ccgccatccc tcctttacgg
     3361 tggtttctga gcagataaag gcgcgtgccg gtgagacatt gctggaaacc gctatcagtc
     3421 tgcagaaagc cgggcttcac acgccggcac agcaggccat tcatctggcc cttcctgtgc
     3481 tggaaagtaa aaacctggcc ttcagcatgg tggacctgct gacagaggcg aagtcgtttg
     3541 ctgcagaagg aaccggtttt gctgacctgg gaggggaaat caatgcgcag ataaaacggg
     3601 gtgatttact gtatgtggat gtggcaaaag gctatggcac aggcctgctg gtttcccgtg
     3661 cgtcgtatga ggcagaaaag agcattcttc gccatattct cgaaggtaag gaggcggtca
     3721 cgccgctgat ggagagagta cctggcgaac tcatggagaa actgacatca ggacagcgcg
     3781 ccgccacccg tatgatactg gaaacgtctg accgtttcac ggtggtgcag ggctatgccg
     3841 gtgtgggtaa gaccacacag ttccgggcgg tgatgtcagc cgtgaacatg ctgccggaga
     3901 gtgaacgtcc ccgtgtcgtg gggctggtgc ccacacaccg tgcggtcggg gagatgcgca
     3961 gcgccggcgt ggatgcacag acactggcgt cctttctgca tgacacgcag ctgcagcagc
     4021 gcagcggaga aactccggat ttcagcaaca cgctgttcct tctcgatgag agctctatgg
     4081 tgggcaatac cgacatggca cgggcatacg ccctgattgc ggccggtggc ggtcgggctg
     4141 tggccagcgg tgacacggac cagctgcagg ccatcgcgcc cggtcagcct ttccgtctcc
     4201 agcagacgcg cagtgctgcc gatgtggtca tcatgaagga gattgtgcgt cagacgccgg
     4261 aactgcggga ggcggtatac agcctgatta accgggatgt ggaaagggcg ctgtccgggc
     4321 ttgagcgtgt gaaaccgtct caggtgccac gtctggaggg cgcatgggca ccggagcact
     4381 ccgtgacgga gttcagtcac agccaggaag cgaaactggc agaagcgcag cagaaggcga
     4441 tgctgaaagg cgaggctttt ccggatgtcc ccatgacact gtatgaagcc attgtccgcg
     4501 actataccgg caggacaccg gaagcacggg agcagacgct gattgtcacg cacctgaatg
     4561 aggaccggcg cgtactgaac agcatgattc atgatgcacg ggaaaaggcc ggagaactgg
     4621 ggaaggtgca ggtcatggtg cctgtcctga acacagcgaa tatacgcgac ggggagctgc
     4681 gtcgtctctc cacctgggag aataacccgg atgcccttgc cctggtggat aatgtgtatc
     4741 accggattgc cggtatcagt aaggatgacg ggctgataac cctgcaggat gcggaaggta
     4801 acacgcggct gatttcgccc cgggaggcgg tggctgaagg cgtcacgctg tacaccccgg
     4861 acaccatccg ggtggggacc ggtgacagga tacgcttcac gaagagtgac cgggagcgcg
     4921 gttatgtggc caacagcgtc tggacggtga cagcggtttc cggtgacagt gtcacacttt
     4981 ctgacggtca gcagacccgt gtgattcgcc ccggtcagga gcgggcagag caacatattg
     5041 acctggccta tgccatcacc gcccacggtg cacagggggc gagtgaaacc tttgccatcg
     5101 cgcttgaagg cacggaaggt aaccggaaac tgatggccgg ctttgagtca gcctacgtgg
     5161 ccctgtcgcg tatgaagcag catgtgcagg tgtacaccga taaccgtcag ggctggacgg
     5221 atgccatcaa caatgccgta cagaaaggaa ctgcccacga tgtgtttgag ccgaaaccgg
     5281 accgggaggt catgaatgca gagcggctgt tcagtacggc gcgggagctg cgggacgtgg
     5341 cggcaggccg tgccgttctc cgtcaggcgg ggctggccgg gggagacagt cctgcacggt
     5401 ttattgctcc gggacgtaaa tatccgcaac cgtatgtggc actgccggcg tttgaccgta
     5461 acggcaagtc cgccggtatc tggctgaacc cgctgaccac ggatgacgga aacgggctgc
     5521 ggggattcag cggtgaaggt cgggtaaaag gcagcgggga tgcgcagttc gtggccctgc
     5581 agggcagccg taacggagaa agcctgctgg ctgataatat gcaggatggc gtccggattg
     5641 cccgtgataa tcctgacagt ggtgtggtgg taagaatcgc cggtgaaggc cgtccgtgga
     5701 atcccggtgc catcaccggt ggccgcgtgt ggggggatat cccggacaac agcgtccagc
     5761 cgggagccgg aaatggtgaa ccggtcacgg cagaggtact ggcacagcgg caggctgaag
     5821 aggccatccg ccgtgaaacg gaacgtcgcg cagatgaaat tgtccgtaaa atggcagaga
     5881 acaaacctga cctgccggac ggcaaaacag agcaggctgt cagggaaatt gccgggcagg
     5941 agcgtgaccg ggctgccata actgaacggg aagccgcgct gccggagagt gtactgcgtg
     6001 aaccacaacg ggtgcgggaa gcggtccggg aagttgcccg ggaaaatttg ctgcaggagc
     6061 gtctgcagca gatggagcgg gatatggttc gtgacctgca gaaagagaaa accccgggcg
     6121 gagactgata caggaagaca aacactgatg acaacggata acacgaacac gacacgtaac
     6181 gattcactgg ctgcacggac cgatacctgg ttgcagtcat ttctggtctg gtcacccgga
     6241 cagcgggata tcatcaaaac ggtggcactg gtgctgatgg tactggacca catcaatctg
     6301 atattccagc tgaagcagga atggatgttt ctggccgggc gaggagcctt tccgcttttt
     6361 gccctggtgt gggggctgaa tctgtcccgt catgcgcata tccggcaacc ggccattaac
     6421 cggctgtggg gatggggaat tattgcccag ttcgcatatt atctggcagg ctttccttgg
     6481 tatgagggga atatcctgtt tgcctttgca gtggcggcac aggtgctgac gtggtgtgaa
     6541 acgcgcagtg ggtggcgtac agcagcggcc atactcctga tggccctgtg ggggcctttg
     6601 tccggcacca gttacggtat tgccgggttg ctgatgctgg cagtcagcaa ccggctgtac
     6661 cgggcggaag acagagcgga acgtctggcg ctggtcgcct gcctgctggc tgttattccg
     6721 gcgcttaacc ttgccaccag tgatgcagcg gcggtggccg gtcttgtgat gacggtgctg
     6781 accgttggtc tggtgttgtg tgccggaaaa tcattacccc gtttctggcc cggagacttt
     6841 ttcccgacgt tctatgcctg tcatctggct gtgctgggcg ttctggcgct gtgacgggtg
     6901 tggtatcttt ggccgcaaga ggatgattcg tcagaggcag aacacagcat gacagagcag
     6961 aagcgaccgg tactgacact gaagcggaag acagagggaa cagcgcctgt ccgcagccgg
     7021 aaaaccatca tcaatgtcac cacgccacca aagtggaagg tgaaaaagca gaagctggcc
     7081 gagaaagcgg cccgtgaagc agagctggcg gcaaaaaaag cgcaggccag acaggcgctg
     7141 tccatttatc tgaacctgcc cacgctggat gaggctgtga ataccctgaa gccctggtgg
     7201 ccgggattat ttgacggtga cacgccccgg cttctggcct gcggtatccg ggacgtgtta
     7261 ctggaagacg tggcgcagcg gaatatcccg ctctcgcata aaaaactgcg cagggcgctg
     7321 aaggccatca cccgttcaga aagctatctg tgtgccatga aagccggtgc ctgccggtat
     7381 gacacggaag ggtatgtgac ggagcatatt tctcaggagg aggaagcgta tgcgggagcg
     7441 cgtctggcga aaattcgtcg ccagaaccgg ataaaggcag aacttcaggc cgtgcttgat
     7501 gagaaataaa aaaagcctcc cccagagaga ggaggcaggg aaataaggct taaaaggaaa
     7561 taacttccgc ttccgaaagg aagccagtcc actgaattat ttatcacaat aatactgaca
     7621 actaaagggg gacaagaaaa tgaatggatt cagaaacagt tcacggaacg gccaggtctg
     7681 gcgttaccag cgtgccggcg ggcgggccgt tattctggaa gtcagtggac gctggatgga
     7741 agcggcagaa gcatggcgac gggctgcctg tgttgcgcct aggacagact ggcagcagtt
     7801 tgcccggaaa agggctgaac actgccaccg gcgctgccgg ggaagggtgt aacgaaaaaa
     7861 gcagccttca ttactctagg agtcagaagg ctgaaaggag tttatgagat ttgcttctta
     7921 atatccggtc gggcagtatg accggacagt tctgagtagc acagagtctt ttccgcatta
     7981 agtcgatttt ttatattttg tgattagttt cacaaaataa ggtgttattc aatgtgtgct
     8041 gcactattca ggggggctga aatacaggtg gcatttatct gatatggaga ataacatgag
     8101 gaaatatatt ccactggcat tatttatctt ttcatggccg gtattgtctg cagatattca
     8161 tggacgggtt gttcgtgtac tggacggtga taccatcgag gtcatggact cactgaaggc
     8221 ggtgcggatc aggctggtaa atattgatgc accggaaaag aaacaggatt atggccggtg
     8281 gtcaacagac atgatgaaat cactggtcgc aggaaaaacg gttaccgtca ca
//
LOCUS       DAZSIO010000163         7937 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZSIO010000163 DAZSIO010000000
VERSION     DAZSIO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.165"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..956)
                     /locus_tag="ACHX5T_003385"
     CDS             complement(<1..956)
                     /locus_tag="ACHX5T_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFR8468442.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="ACHX5T_003386"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="ACHX5T_003386"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFR8468443.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="ACHX5T_003387"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="ACHX5T_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="ACHX5T_003388"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="ACHX5T_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR8468444.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="ACHX5T_003389"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="ACHX5T_003389"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR8468445.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="ACHX5T_003390"
     CDS             complement(5018..6346)
                     /locus_tag="ACHX5T_003390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR8468446.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..7937)
                     /locus_tag="ACHX5T_003391"
                     /pseudo
     CDS             complement(6354..7937)
                     /locus_tag="ACHX5T_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616724.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
BASE COUNT         2104 a         2010 c         1789 g         2034 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctgatgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaagca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttaacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaat
//
LOCUS       DAZSIO010000164         7909 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZSIO010000164 DAZSIO010000000
VERSION     DAZSIO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.166"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(123..953)
                     /locus_tag="ACHX5T_003392"
     CDS             complement(123..953)
                     /locus_tag="ACHX5T_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HFR8468447.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1163..2176
                     /gene="intI1"
                     /locus_tag="ACHX5T_003393"
     CDS             1163..2176
                     /gene="intI1"
                     /locus_tag="ACHX5T_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR8468448.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2379..2729
                     /locus_tag="ACHX5T_003394"
                     /pseudo
     CDS             2379..2729
                     /locus_tag="ACHX5T_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            2855..3415
                     /locus_tag="ACHX5T_003395"
     CDS             2855..3415
                     /locus_tag="ACHX5T_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR8468449.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3418..6384
                     /locus_tag="ACHX5T_003396"
     CDS             3418..6384
                     /locus_tag="ACHX5T_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFR8468450.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6451..6828
                     /locus_tag="ACHX5T_003397"
     CDS             6451..6828
                     /locus_tag="ACHX5T_003397"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8468451.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7029..7688)
                     /locus_tag="ACHX5T_003398"
     CDS             complement(7029..7688)
                     /locus_tag="ACHX5T_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFR8468452.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1753 a         2241 c         2133 g         1782 t
ORIGIN      
        1 cttccctcat gatgtttaac gtttgaaata aggggcgccg agcgccagcg aggggagcca
       61 aaagcttgct tttggccgtc ccgacttgat tgaagggttg ggcgattttg ccattagatt
      121 ttttataaat ttagtgtgtt tagaatggtg atcgcatttt tcttggcttt tatgcttgat
      181 gttaaattcg accccaagtt tcctgtaagt gcggacacaa aaacatattt atgtcctgat
      241 ttgcttataa taaacccttc aaaccatccg ttttgtaagg ttctatttgc tgtgaatcct
      301 gcaccagttt tcccatacag ttttgtacta ttatccagat cttgtagata catgttctct
      361 atggtgtttt ctatggctga gtttttaact gggagattgt gattaataat tttacgcagg
      421 aattgaattt gttcttctgg tgaaattttt aagctacttt cgagccatgc ttctgttaat
      481 ccgttgtttc tttctttatc tccagagaag tcttgatttc cataatcaaa atctttgaga
      541 taattcttga ttttatttaa tccaattttt tgggttattt cttgcgaaac ccaaacaaca
      601 gaaaattgca tccacgtctt tggtgtatga ttgctgttcc agatctccat tcctttgggg
      661 gttttatccc atttgaatat ggttttctga tctattattt ccgcatcaaa tgccataagt
      721 gataatgcga tcttgaaagt tgaatctggt gccatttgcg ttgcacactt tgctttattg
      781 aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta aaaaacaacc ttcagttcct
      841 tcaaataatg gagatgcaac agtagagata tctgttgatg cactggcgct gctgtagata
      901 atatttgcaa ttattaaaaa aatagcgaag ttgatatgta ttgtgttttt cataataagt
      961 attggtttgg taaagggctt aattttaacg gctaacaatt aatgaggctc cgggttcgcc
     1021 caactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1081 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     1141 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     1201 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
     1261 cgaacaggct tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
     1321 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
     1381 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt
     1441 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
     1501 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
     1561 tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg atcagtgagg gtttgcaact
     1621 gcgggtcaag gatctggatt tcgatcacgg cacgatcatc gtgcgggagg gcaagggctc
     1681 caaggatcgg gccttgatgt tacccgagag cttggcaccc agcctgcgcg agcagctgtc
     1741 gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc cgcagcggcg ttgcgcttcc
     1801 cgacgccctt gagcggaagt atccgcgcgc cgggcattcc tggccgtggt tctgggtttt
     1861 tgcgcagcac acgcattcga ccgatccacg gagcggtgtc gtgcgtcgcc atcacatgta
     1921 tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa caagcaggca tcacgaagcc
     1981 cgccacaccg cacaccctcc gccactcgtt cgcgacggcc ttgctccgca gcggttacga
     2041 cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc tctacgacga tgatttacac
     2101 gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg cttgatgcgc tgccgcccct
     2161 cactagtgag aggtagggca gcgcaagtca atcctggcgg attcactacc cctgcgcgaa
     2221 ggccatcggt gccgcatcga acggccggtt gcggaaagtc ctccctgcgt ccgctgatgg
     2281 ccggcagcag cccgtcgttg cctgatggat ccaacccctc cgctgctata gtgcagtcgg
     2341 cttctgacgt tcagtgcagc cgtcttctga aaacgacaat ggaggtggta gccgagggtg
     2401 tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg ttgcgacacg gtgcagggtt
     2461 tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg cttcgtcaac caatggagga
     2521 acaccaccat gaacgccaat gaaccgagca ccagttgctg cgtgtgctgc aaggaaatcc
     2581 cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt ggagcatttc tgcgggctgg
     2641 agtgctatca gcgcttccag gcgcgggcca gcactgcgac cgaaaccagc gtcaaaccgg
     2701 acgcttgtga ttcgccgccg tcaggttgag gcatacccta acctgatgtc agatgccatg
     2761 tgtaaattgc gtcaggatag gattgaattt tgaatttatt gacatatctc gttgaaggtc
     2821 atagagtctt ccctgacatt ttgcagggaa ttccatgact ggacagcgca ttgggtatat
     2881 cagggtcagc accttcgacc agaacccgga acggcaactg gaaggcgtca aggttgatcg
     2941 cgcttttagc gacaaggcat ccggcaagga tgtcaagcgt ccgcaactgg aagcgctgat
     3001 aagcttcgcc cgcaccggcg acaccgtggt ggtgcatagc atggatcgcc tggcgcgcaa
     3061 tctcgatgat ttgcgccgga tcgtgcaaac gctgacacaa cgcggcgtgc atatcgaatt
     3121 cgtcaaggaa cacctcagtt ttactggcga agactctccg atggcgaacc tgatgctctc
     3181 ggtgatgggc gcgttcgccg agttcgagcg cgccctgatc cgcgagcgtc agcgcgaggg
     3241 tattgcgctc gccaagcaac gcggggctta ccgtggcagg aagaaatccc tgtcgtctga
     3301 gcgtattgcc gaactgcgcc aacgtgtcga ggctggcgag caaaagacca agcttgctcg
     3361 tgaattcgga atcagtcgcg aaaccctgta tcaatacttg agaacggatc agtaaatatg
     3421 ccacgtcgtt ccatcctgtc cgccgccgag cgggaaagcc tgctggcgtt gccggactcc
     3481 aaggacgacc tgatccgaca ttacacattc aacgataccg acctctcgat catccgacag
     3541 cggcgcgggc cagccaatcg gctgggcttc gcggtgcagc tctgttacct gcgctttccc
     3601 ggcgtcatcc tgggcgtcga tgaactaccg ttcccgccct tgttgaagct ggtcgccgac
     3661 cagctcaagg tcggcgtcga aagctggaac gagtacggcc agcgggagca gacccggcgc
     3721 gagcacctga gcgagctgca aaccgtgttc ggtttccggc ccttcaccat gagccattac
     3781 cggcaggccg tccagatgct gaccgagctg gcgatgcaaa ccgacaaagg catcgtgctg
     3841 gccagcgcct tgatcgggca cctgcggcgg cagtcggtca ttctgcccgc cctcaacgcc
     3901 gtcgagcggg cgagtgccga ggcgatcacc cgtgctaacc ggcgcatcta cgacgccttg
     3961 gccgaaccac tggcggacgc gcatcgccgc cgcctcgacg atctgctcaa gcgccgggac
     4021 aacggcaaga cgacctggtt ggcttggttg cgccagtctc cggccaagcc aaattcgcgg
     4081 catatgctgg aacacatcga acgcctcaag gcatggcagg cactcgatct gcctaccggc
     4141 atcgagcggc tggttcacca gaaccgcctg ctcaagattg cccgcgaggg cggccagatg
     4201 acacccgccg acctggccaa attcgagccg caacggcgct acgccactct cgtggcgctg
     4261 gccaccgagg gcatggccac cgtcaccgac gaaatcatcg acctgcacga ccgcatcctg
     4321 ggtaagctgt ttaacgctgc caagaataag catcagcagc agttccaggc gtcaggcaag
     4381 gccatcaacg ccaaggtacg tctgtacggg cgcatcggtc aggcgctgat cgacgccaag
     4441 caatcaggcc gcgatgcgtt tgccgccatc gaggccgtca tgtcctggga ttcctttgcc
     4501 gagagcgtca ccgaggcgca gaagctcgcg caacccgatg acttcgattt cctgcatcgc
     4561 atcggcgaga gctacgccac cctgcgccgc tatgcaccgg aattccttgc cgtgctcaag
     4621 ctgcgggccg cgcccgccgc caaaaacgtg cttgatgcca ttgaggtgct gcgcggcatg
     4681 aacaccgaca acgcccgcaa gctgccagcc gatgcaccga ccggcttcat caagccgcgc
     4741 tggcagaaac tggtgatgac cgacgccggc atcgaccggc gctactacga actgtgcgcg
     4801 ctgtccgagt tgaagaactc cctgcgctcg ggcgacatct gggtgcaggg ttcacgccag
     4861 ttcaaggact tcgaggacta cctggtaccg cccgagaagt tcaccagcct caagcagtcc
     4921 agcgaattgc cgctggccgt ggccaccgac tgcgaacaat atctgcatga gcggctgacg
     4981 ctgctggaag cacaacttgc caccgtcaac cgcatggcgg cagccaacga cctgccggat
     5041 gccatcatca ccgagtcggg cttgaagatc acgccgctgg atgcggcggt gcccgacacc
     5101 gcgcaggcgc tgatagacca gacagccatg gtcctgccgc acgtcaagat caccgaactg
     5161 ctgctcgaag tcgatgagtg gacgggcttc acccggcact tcacgcactt gaaatcgggc
     5221 gatctggcca aggacaagaa cctgttgttg accacgatcc tggccgacgc gatcaacctg
     5281 ggcctgacca agatggccga gtcctgcccc ggcacgacct acgcgaagct cgcttggctg
     5341 caagcctggc atacccgcga cgaaacgtac tcgacagcgt tggctgaact ggtcaacgct
     5401 cagtttcggc atccctttgc cgggcactgg ggcgatggca ccacatcatc atcggacgga
     5461 cagaatttcc gaaccgctag caaggcaaag agcacggggc acatcaaccc aaaatatggc
     5521 agcagcccag gacggacttt ctacacccac atctccgacc aatacgcgcc attccacacc
     5581 aaggtggtca atgtcggcct gcgcgactca acctacgtgc tcgacggcct gctgtaccac
     5641 gaatccgacc tgcggatcga ggagcactac accgacacgg cgggcttcac cgatcacgtc
     5701 ttcgccctga tgcacctctt gggcttccgc ttcgcgccgc gcatccgcga cctgggcgac
     5761 accaagctct acatcccgaa gggcgatgcc gcctatgacg cgctcaagcc gatgatcggc
     5821 ggcacgctca acatcaagca cgtccgcgcc cattgggacg aaatcctgcg gctggccacc
     5881 tcgatcaagc agggcacggt gacggcctcg ctgatgctca ggaaactcgg cagctacccg
     5941 cgccagaacg gcttggccgt cgcgctgcgc gagttgggcc gcatcgagcg cacgctgttc
     6001 atcctcgact ggctgcaaag cgtcgagcta cgccgccgcg tgcatgccgg gctgaacaag
     6061 ggcgaggcgc gcaatgcgct ggcccgtgcc gtgttcttca accgccttgg tgaaatccgt
     6121 gaccgcagtt tcgagcagca gcgctaccgg gccagcggcc tcaacctggt gacggcggcc
     6181 atcgtgctgt ggaacacggt ctacctggag cgtgcggcgc atgcgttgcg cggcaatggt
     6241 catgccgtcg atgactcgct attgcagtac ctgtcgccac tcggctggga gcacatcaac
     6301 ctgaccggtg attacctatg gcgcagcagc gccaagatcg gcgcggggaa gttcaggccg
     6361 ctacggcctc tgcaaccggc ttagcgtgct ttattttccg ttttctgaga cgacccctat
     6421 tatggcgaag gtatcattca ggaagcgttg atgaaggatt atctctgtaa aaagttattc
     6481 aaccggcttt ccggtaccct ggtgatcaga gcgcgctgcg gcaataacat tactggcctg
     6541 gcatgttgca atattcttta tccctcgccc cgatacagcg gtcagctgca tattaaagag
     6601 ctgtatgttt ctcagtgcga tcgaaataag ggcacgggta aagcgataat gcgctttata
     6661 gcccggcttg cgcttgaaca ggaatgcctt agccttagct ggaacgctga aaaatccaac
     6721 cccggcgcta accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat
     6781 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
     6841 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     6901 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     6961 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
     7021 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     7081 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     7141 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     7201 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     7261 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     7321 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     7381 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     7441 accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca
     7501 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     7561 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     7621 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     7681 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     7741 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     7801 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat
     7861 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgt
//
LOCUS       DAZSIO010000165         7886 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZSIO010000165 DAZSIO010000000
VERSION     DAZSIO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7886
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.167"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            207..710
                     /gene="dps"
                     /locus_tag="ACHX5T_003399"
                     /gene_synonym="pexB"
     CDS             207..710
                     /gene="dps"
                     /locus_tag="ACHX5T_003399"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFR8468453.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1114..1860
                     /gene="glnH"
                     /locus_tag="ACHX5T_003400"
     CDS             1114..1860
                     /gene="glnH"
                     /locus_tag="ACHX5T_003400"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR8468454.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            1999..2658
                     /gene="glnP"
                     /locus_tag="ACHX5T_003401"
     CDS             1999..2658
                     /gene="glnP"
                     /locus_tag="ACHX5T_003401"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR8468455.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2655..3377
                     /gene="glnQ"
                     /locus_tag="ACHX5T_003402"
     CDS             2655..3377
                     /gene="glnQ"
                     /locus_tag="ACHX5T_003402"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR8468456.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            3638..5862
                     /gene="ybiO"
                     /locus_tag="ACHX5T_003403"
                     /pseudo
     CDS             3638..5862
                     /gene="ybiO"
                     /locus_tag="ACHX5T_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(5859..6785)
                     /gene="rlmF"
                     /locus_tag="ACHX5T_003404"
     CDS             complement(5859..6785)
                     /gene="rlmF"
                     /locus_tag="ACHX5T_003404"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR8468457.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            7061..7321
                     /gene="mcbA"
                     /locus_tag="ACHX5T_003405"
     CDS             7061..7321
                     /gene="mcbA"
                     /locus_tag="ACHX5T_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR8468458.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            7586..>7886
                     /locus_tag="ACHX5T_003406"
     CDS             7586..>7886
                     /locus_tag="ACHX5T_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR8468459.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT         1920 a         2019 c         1975 g         1972 t
ORIGIN      
        1 tatttattcc gaaaattcct ggcgagcaga taaataagaa ttgttcttat caatatatct
       61 aactcattga atctttatta gttttgtttt tcatgcttgt taccactatt agtgtgatag
      121 gaacagccag aatagcggaa cacatagccg gtgctatact tagtctcgtt aattactggg
      181 acataacatc aagaggatat gaaattatga gtaccgctaa attagttaaa tcaaaagcga
      241 ccaatctgct ttatacccgc aacgatgtct ccgacagcga gaaaaaagca acagtagagt
      301 tgctgaatcg ccaggttatc cagtttattg atctttcttt gattaccaaa caagcgcact
      361 ggaacatgcg cggcgctaac ttcattgccg tacatgaaat gctggatggc ttccgcaccg
      421 cactgatcga tcatctggat accatggcag aacgtgccgt gcagctgggc ggtgtagctc
      481 tggggaccac tcaagttatc aacagcaaaa ccccgctgaa aagttacccg ctggacatcc
      541 acaacgttca ggatcacctg aaagaactgg ctgaccgtta cgcaatcgtc gctaatgacg
      601 tacgcaaagc gattggcgaa gcgaaagatg acgacaccgc agatatcctg accgccgcgt
      661 ctcgcgacct ggataaattc ctgtggttta tcgagtctaa catcgaataa atccatcgct
      721 gatggtgcag aactttagta cccgataaaa gcggcttcct gacaggaggc cgttttgttt
      781 tgcagcccac ctcaacgcac ttatttagtg catccatctg ctatccccag ctgattaagt
      841 aaattttttg tatccacatc atcacacaat cgttacataa agattgtttt ttcatcaggt
      901 tttacgctaa ataatcactg tgttgagtgc acaattttag cgcaccagat tggtgcccca
      961 gaatggtgca tcttcagggt attgccctat aaatcgtgca tcacgttttt gccgcatctc
     1021 gaaaaatcaa tgagttgcaa aattggcacg attttttcat atatgtgaat gtcacgcagg
     1081 ggatcgtccc gtggatagaa aaaaggaaat gctatgaagt ctgtattaaa agtttcactg
     1141 gctgcactga ccctggcttt tgcggtttct tctcatgccg cggataaaaa attagttgtc
     1201 gcgacggata ccgccttcgt tccgtttgaa tttaaacagg gcgataaata tgtgggcttt
     1261 gacgttgatc tgtgggctgc catcgctaaa gagctgaagc tggattacga actgaagccg
     1321 atggatttca gtgggatcat tccggcactg caaaccaaaa acgtcgatct ggcgctggcg
     1381 ggcattacca tcaccgacga gcgtaaaaaa gcgatcgatt tctctgacgg ctactacaaa
     1441 agcggcctgt tagtgatggt gaaagctaac aataacgatg tgaaaagcgt gaaagatctc
     1501 gacgggaaag tggttgctgt gaagagcggt actggctccg ttgattacgc gaaagcaaac
     1561 atcaaaacta aagatctgcg tcagttcccg aacatcgata acgcctatat ggaactgggc
     1621 accaaccgcg cagacgccgt tctgcacgat acgccaaaca ttctgtactt catcaaaacc
     1681 gccggtaacg gtcagttcaa agcggtaggt gactctctgg aagcgcagca atacggtatt
     1741 gcgttcccga aaggtagcga cgagctgcgt gacaaagtca acggcgcgtt gaaaaccctg
     1801 cgcgagaacg gaacttacaa cgaaatctac aaaaaatggt tcagtactga accgaaataa
     1861 taacgctaca cctgtaaagc gcactggcag ttccctctcc cccctgggga gaggattagg
     1921 gtgaggggcg caaacccgct ccggggccat taattaccct gaatttgatt atttacacca
     1981 cggtaacagg aacaacatat gcagtttgac tggagtgcca tctggcctgc cattccgctt
     2041 ctgattgaag gtgccaaaat gaccctgtgg atttcggtcc tcggtctggc tggcggtctg
     2101 gtaatcggat tgctggcagg ttttgcacgc accttcggtg gttggatagc caaccacgtc
     2161 gcgctggtct ttattgaagt gatccgcggc acacctatcg tcgtccaggt gatgtttatc
     2221 tatttcgccc tgccgatggc gtttaacgac ttacgcatcg acccatttac tgcggcggtg
     2281 gtcaccatca tgatcaactc cggcgcgtac attgcggaaa tcacgcgtgg tgcggtgctg
     2341 tctatccaca aaggttttcg tgaagcagga ctggcgctcg gtctttcacg ttgggaaacc
     2401 attcgctacg tcattttacc gctggcactg cgtcgtatgc tgccgccgct gggtaaccag
     2461 tggatcatca gcattaaaga cacctcgctg tttattgtta tcggcgtggc ggaactgacc
     2521 cgtcaggggc aagaaattat tgccggtaac ttccgcgccc ttgagatctg gagcgccgtg
     2581 gcggtgttct atctgattat taccctggtg ctgagcttta ttctgcgtcg tctggaaaga
     2641 aggatgaaaa tcctgtgatt gaatttaaaa acgtctccaa gcactttggc ccaacccagg
     2701 tgctgcacaa tatcgatttg aacattgccc agggcgaagt cgtggtgatt atcgggccgt
     2761 ccggttccgg taaatcgacc ctgctgcgct gcatcaacaa actggaagaa atcacctccg
     2821 gcgatctgat tgtcgatggc ctgaaggtca acgatccgaa agttgacgag cgcctgattc
     2881 gccaggaagc aggtatggtg ttccagcagt tttacctctt cccgcatctg acggcgctgg
     2941 gaaacgtcat gtttggtccg ctacgcgtgc gtggcgcgaa caaagaagag gcggaaaaac
     3001 tggcgcgtga gctgctggcg aaagtcggtc tggcagaacg tgcacatcac tacccttccg
     3061 aactttctgg tggtcaacag cagcgtgtgg cgattgcccg cgcgctggcg gtgaagccga
     3121 aaatgatgct gtttgatgaa ccgacttccg ctcttgaccc ggaactgcgc catgaagtgc
     3181 tgaaggttat gcaggatctg gctgaagaag ggatgacgat ggtgatcgtg acccacgaaa
     3241 tcggttttgc cgagaaagta gcttcgcggc tgatctttat cgacaaaggc cggattgcgg
     3301 aagatggcaa tccgcaggtg ttgatcaaga acccgccgag ccagcgcttg caggaatttt
     3361 tgcagcacgt ctcttaataa gacacattgc ctgatgcgct acgcttatca ggcctacagg
     3421 atatctggca acttattaaa attgcatgaa cttgtaggac ggataaggcg ttcacgccgc
     3481 atccggcaaa aaagcccgca cgttgtcagc aacctgctta atatcccttc ctccctttca
     3541 cccgaaaggg aggcacacca gattcctctc atttaaaatc gcccctcccc cagcatctat
     3601 acttatcttt ttgctctgtt ttctcactgg aggagtcatg cggtggatcc tgttcatcct
     3661 cttctgcctg ctgggcgcac ctgcccacgc ggtatccata cccggcgtta caaccacaac
     3721 gacaacggac tcaacgactg aaccggcccc ggaaccggat atcgaacaaa aaaagcggcc
     3781 tatggcgcac tggcggatgt gctggataac gacacctcgc gtaaagagtt gatcgaccag
     3841 ttgcgtactg tcgccgcgac gccccctgcg gaaccggtcc cgaagatcgt gccgccgacg
     3901 ctggttgaag agcaaaccgt gctgcaaaag gtcaccgaag tcagccgcca ttatggtgaa
     3961 gccctttccg cccgcttcgg gcaactttat cgcaatatca ccggctcccc gcataagcca
     4021 tttaatccac aaaccttcag caatgcgctg agccattttt caatgttagc ggtattagtg
     4081 tttggttttt actggctgat tcgcctgtgc gcactgccgc tatatcgcaa aatgggtcag
     4141 tgggcgaggc aaaaaaatcg tgagcgcagc aactggttgc agcttccggc gatgattatt
     4201 ggggcgttta ttatcgacct gctgttactg gccctgacat tatttgtcgg ccaggtatta
     4261 agcgacaacc tgaatgcagg cagtcgcacc atcgctttcc aacaaagttt gtttctcaac
     4321 gcctttgccc tgattgaatt tttcaaagcc gtactacgcc tgattttttg cccaaacgtg
     4381 gcggagctgc gcccgttcac gattcaggac gagagcgccc gttactgggg tcgtcgcctg
     4441 agctggttaa gcagcctgat tggctatggc ctgattgtgg ccgtgccgat tatctctaat
     4501 caggtgaatg tacagatagg tgcgctggcg aacgtcatca ttatgctgtg catgaccgtc
     4561 tgggcgttgt acctgatctt ccgaaataaa aaagagatta cccagcatat gctcaacttt
     4621 gcggagcatt cgctggcttt tttcagcctg tttatccgcg cctttgcgct ggtgtggcac
     4681 tggctggcaa gcgcctattt tatcatgctg tttttctttt cgttgttcga tccgggcaac
     4741 agtctgaaat ttatgatggg tgcaacggtg cgcagcctgg cgattattgg tatcgcagcg
     4801 tttgtttccg gtatgttttc ccgctggctg gcgaaaacca tcaccctctc gccacatact
     4861 cagcgtaact atccggagct acaaaaacgg ctgaatggct ggctttcggc ggcgctgaaa
     4921 acggcgcgta ttctgaccgt ctgcgtggca gtaatgttgc tgttgagcgc atggggattg
     4981 ttcgatttct ggaactggct gcaaaacggc gcggggcaga aaaccgtaga tatcctgatc
     5041 cgcatcgcac tcattctttt cttctcggcg gttggctgga cggtgctcgc cagtttgatc
     5101 gaaaaccggc tggcttcgga tattcatggc cgcccgctac ccagcgcccg tacgcgtacc
     5161 ctgctgacgc tgttccgtaa cgcgctggcg gtgattatca gtaccatcac catcatgatt
     5221 gtgttgtcgg aaatcggcgt caatatcgcg ccattgctgg caggtgccgg ggcattaggt
     5281 ctggctatct cgtttggttc gcaaacgctg gtaaaagata ttatcaccgg ggtatttatt
     5341 cagtttgaaa acggcatgaa cactggagat ttggtgacta tcgggccgtt gaccggcaca
     5401 gtggaacgga tgtcgattcg ctccgtgggc gtgcgccagg ataccggggc gtatcacatc
     5461 attccgtggt cttcgataac cacctttgct aacttcgtcc gcggtattgg ttcggtagtg
     5521 gcaaactacg atgttgatcg ccatgaagat gctgataaag ccaatcaggc actgaaagat
     5581 gcggtaacgg aattgatgga aaacgaagaa attcgcgggc tgattattgg tgaaccgaac
     5641 tttgccggga ttgtcggctt aagcaatacc gcgtttacac tgcgtgtttc gttcaccacg
     5701 ctgccactca aacagtggac ggtacgcttt gccctcgaca gccaggtgaa aaaacatttc
     5761 gacctggcgg gcgttcgcgc gccagtgcag acttatcagg tgctgcctgc tccgggcgcg
     5821 accccggctg aaccgctgcc gccgggggag ccaacgcttt aacgttggcg attaacaaaa
     5881 cggcggcgct gctcgtcgtt cataaaggtc caggcaataa agcgactctg cttttgcccc
     5941 tgggccatct ctttttttac caccttcacc gcgcccacgt cggtcagagc acgatacaac
     6001 ggcggtaagt tttcaccacg agataccagc gaggtaaacc acatcacctg cttcgcgaag
     6061 cctttgctct cttcaatcat ctttttgata aagttgactt caccgccttc acaccacaac
     6121 tcctgttgct gaccaccaaa gttcagtgca tcgtctttgt tcagccccag gttacggcgt
     6181 ttacgctcac tacctgcccg cgccgcagcg gcggaatcgt ggaatggcgg gttacacaag
     6241 gtcgcgtcgt attgctcgtt tttgtggatg atgccgttaa aaatcgcccc actctctttt
     6301 tgccgacgca gacgaatggc gcggttaagc cccggattag cactgataat cgcctgcgca
     6361 ctgcttaacg cctggctgct ggtttcacta ccggtaaaac gccagccata ttcatgtacg
     6421 ccaatcagcg gataaataca gttcgcacca acgccgatat ccagaatgct ggcattcgct
     6481 ggaattgttc cgcttgcttc agccagtaaa tcggcaaggt gatgaatata atccgcccgg
     6541 cccggtactg gtgggcagag aaaaccgtcg gggatatccc agttcgctac ggcgtaaaaa
     6601 tgggccagca acgccttatt gagcgccttc accgccagcg gattggcaaa gtctacgctt
     6661 tgctcccctg cgggtgtaag cgtgaggaat tgcctgagtt caggattgac ctgacaaagc
     6721 gtggcgagat cgtagcggct gtgatgacgg ttgcgcggat gcaaccccgg tttctgggcg
     6781 gacatagcat tctcctttaa gcagcggcgt aagatacccg cataacgcga cagggtaaat
     6841 atctggctgc gtttatcatt ccccaaaacc ttaacatggc tatggtttat ggcaattcat
     6901 cataaaaatc agcattatgc atgtcattga tttgtcttat tttgtaaaaa tatatcgttc
     6961 aaaaaataag catttctctg tcaatttttt gcgtcatttg catcgcgcag gcgacctaca
     7021 cttatcaggc actacccgtt gtatttatga ggatgtcatt atgaaaaagt gcctcacact
     7081 actgattgcc accgtcctga gcggaatctc tctcacggct tatgccgcgc aaccgatgag
     7141 taacctggac agcggtcagc tacggcccgc cggtaccgtt tcggcaactg gcgcatcaaa
     7201 cctaagcgat ctggaggata aactggcaga aaaagcgcgc gaacaaggcg cgaaaggtta
     7261 tgtcattaat tccgcaggcg gaaatgacca gatgttaggt actgcaacca tctacaaata
     7321 accccaccaa cgtaactgca ttcgcctctg gatgcagttt tcgcctgttt tgtaatcaaa
     7381 aaacatcaaa tatgaacgca atgtaaataa atgtatttct ttttcgcgca atgggtgata
     7441 gaaaatcgca tcaagtgata atgcttatca aaattattat cacttgcacg aacactatca
     7501 cgggattaac agtggcatcg catccgcaga gatgctttct cgtggcagtg aaaatttcaa
     7561 catataagaa aaagtcacct gcaaaatgga aaacaatcgc aatttccctg ccagacaatt
     7621 tcattcgctc acgttctttg ccggtctttg tattggtatt acgcctgtgg cacaggcact
     7681 cgccgccgaa gggcaagcta acgcggatga cacgctggtt gtcgaagcat caacgccttc
     7741 gctttatgcg ccacaacaat ctgccgatcc gaaattctcg cgtccggtag cggatactac
     7801 ccgcacgatg acggtgattt ctgaacaagt gattaaagat cagggcgcaa ccaaccttac
     7861 cgacgcgctc aaaaacgtcc ccggcg
//
LOCUS       DAZSIO010000166         7746 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZSIO010000166 DAZSIO010000000
VERSION     DAZSIO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.168"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..1016
                     /gene="ydfJ"
                     /locus_tag="ACHX5T_003407"
     CDS             <1..1016
                     /gene="ydfJ"
                     /locus_tag="ACHX5T_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFR8468460.1"
                     /translation="ILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMG
                     TNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEK
                     VNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAI
                     PTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPS
                     TIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAG
                     IFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPS
                     RSL"
     gene            1105..2565
                     /locus_tag="ACHX5T_003408"
     CDS             1105..2565
                     /locus_tag="ACHX5T_003408"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR8468461.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2601..2804)
                     /gene="ydfZ"
                     /locus_tag="ACHX5T_003409"
     CDS             complement(2601..2804)
                     /gene="ydfZ"
                     /locus_tag="ACHX5T_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR8468462.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2982..3668)
                     /gene="rspR"
                     /locus_tag="ACHX5T_003410"
                     /gene_synonym="ydfH"
     CDS             complement(2982..3668)
                     /gene="rspR"
                     /locus_tag="ACHX5T_003410"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR8468463.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3757..4503)
                     /gene="ydfG"
                     /locus_tag="ACHX5T_003411"
     CDS             complement(3757..4503)
                     /gene="ydfG"
                     /locus_tag="ACHX5T_003411"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR8468464.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4640..6685
                     /gene="dcp"
                     /locus_tag="ACHX5T_003412"
     CDS             4640..6685
                     /gene="dcp"
                     /locus_tag="ACHX5T_003412"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR8468465.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6730..7248)
                     /locus_tag="ACHX5T_003413"
     CDS             complement(6730..7248)
                     /locus_tag="ACHX5T_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR8468466.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7524..>7746
                     /gene="ydeI"
                     /locus_tag="ACHX5T_003414"
     CDS             7524..>7746
                     /gene="ydeI"
                     /locus_tag="ACHX5T_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFR8468467.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHK"
BASE COUNT         1941 a         1814 c         1956 g         2035 t
ORIGIN      
        1 ccatcttgct ggtgacgctg cgtattattc agggattggg tgcaggtgcg gaaatttccg
       61 gtgccggtac gatgctggcg gaatatgcgc caaaaggtaa gcgcggaatt atctcctcat
      121 ttgtggctat gggaactaac tgcggcacct taagcgcaac ggcaatctgg gcctttatgt
      181 tcttcattct cagtaaagag gaactgctgg cgtggggatg gcgtataccg ttcctggcga
      241 gcgttgtcgt gatggtcttt gctatctggt tgcgtatgaa tctgaaagaa agcccggtct
      301 ttgagaaggt taacgacagc aaccaaccga cagcaaaacc tgcacctgct ggtagcatgt
      361 tccagagcaa atccttctgg ctggcaacag ggctgcgttt tggtcaggcg ggtaactccg
      421 gtttaattca gactttcctt gcaggctatt tagtacagac gttattgttt aacaaagcaa
      481 ttccaacaga tgcattgatg atcagttcga ttctcggctt tatgaccatt ccgttccttg
      541 gttggttatc cgataaaatt ggtcgccgga tcccgtatat tattatgaat acctctgcga
      601 ttgtgctggc gtggccaatg ctttctatca tcgtagataa aagctatgcc ccgagcacca
      661 ttatggttgc actgattgtg attcataact gtgcggtgct gggattattt gctctggaaa
      721 acattaccat ggcagaaatg ttcggctgta aaaaccgctt cacccggatg gctatttcaa
      781 aagaaattgg tggtcttatc gcttccggtt ttggtcctat cctggcgggt attttctgca
      841 ccatgacgga atcctggtat ccgatcgcca ttatgatcat ggcatattca gtgattggtt
      901 taatctctgc gctgaaaatg ccagaggtga aagaccgtga tttaagtgcg ctggaagacg
      961 ctgcggaaga tcaaccgcgt gttgtaagag ctgcgcaacc ttccagaagt ctgtaaaccc
     1021 tcaatccctt ctcttaccgg agaggggatt tttattcata taacaaaaca tatagcttgc
     1081 catatttaaa tttaaggaat tgttatggga aataatttgt tatcagcaaa agcgacactc
     1141 cctgtttatg atcgtaataa cctggcccca agaattgttc atttaggctt tggtgcattt
     1201 caccgtgcgc atcagggcgt gtatgccgat attcttgcta cagaacattt cagtgactgg
     1261 ggatattatg aggtcaactt aatcggcggc gaacagcaaa ttgccgattt acaacagcaa
     1321 gataatcttt ataccgttgc ggaaatgtcg gccgatgcgt ggacggctcg cgtcgttggc
     1381 gtcgttaaaa aagccttgca cgtacagatg gatggcttag aaaccgtgtt ggctgcgatg
     1441 tgtgaaccgc aaatcgcgat tgtctctctg acaatcaccg aaaaagggta tttccactct
     1501 ccggcgaccg gacagttaat gctcgagcac ccgatggtcg ctgccgacgt gcaaaatccc
     1561 caccagccga aaacagcaac aggggtgatt gttgaggcgc tggctcgccg taaagcggca
     1621 ggacttcccg catttaccgt catgtcatgt gacaacatgc cagaaaacgg tcatgttatg
     1681 cgtgacgttg tcacttccta cgcgcaagcc gttgatgtaa aactggcaca atggatcgaa
     1741 gataacgtga ctttcccatc aacaatggtg gaccgtattg tgcccgcagt gacagaggat
     1801 acgctggcga aaatcgaaca acttaccggt gtgcgcgatc ctgctggcgt tgcctgtgaa
     1861 cctttccgcc agtgggttat cgaagataac tttgtagccg gacgtccgga atgggaaaaa
     1921 gcgggagccg aactggttag tgatgtgctg ccttatgaag agatgaagtt gcgcatgctc
     1981 aacggcagtc attcattcct ggcatatctg ggttatcttg ctggatatca gcacattaat
     2041 gactgtatgg aagatgaaca ttatcgtcat gcggcgtatg gcatgatgtt gcaggaacaa
     2101 gcgccgacgc tgaaagtgca gggcgttgat ttgcaagatt acgctaaccg attaattgca
     2161 cgctatagca acccggcgtt acgtcatcga acctggcaga ttgcgatgga tggcagccag
     2221 aaattgccac agcggatgtt ggattctgtt cgctggcatc tggcgcatga cagcaagttc
     2281 gatctgctgg cgctgggcgt cgcgggttgg atgcgttatg tcggtggtgt tgatgaacag
     2341 ggaaatccga tagaaatcag tgacccactg ttacctgtta ttcagaaggc tgtacaaagt
     2401 agtgccgaag ggaaagcgcg cgtccagtca ttgctggcga ttaaggcaat ctttggtgat
     2461 gatttgccag acaatagctt gtttactgca aaagtgacgg aagcgtactt gtctttatta
     2521 gcgcatggcg cgaaagcgac cgtggcgaaa tattccgtga agtaaaaaaa gcaatgccgc
     2581 catcggcggc attcacacac ttagttcatg ccgagacgaa tcaggtccag aggtgccagt
     2641 ttctcttcac aaccttcaac aactacggtt tttccgcggc ggatttgctc agcggtcaga
     2701 ccttcagtat caatcgacag gatcttgcca gtgtgaccgg tgccgctaac cataacacgg
     2761 ctgccagtgg tgattgcgtt acggttacga tcgtaggtcg tcataggtgt tttctccttt
     2821 ctgatttaca gtcgaagccc acgtaacggg cgcaatataa atactcctgt cgagtgaaat
     2881 attttttgtt tttgatcaag ctcacatctt ttttgatgca tgcatgagcc gtaaggcggg
     2941 gggaaaagcc tctcatcccg gatgggatga gagtaaatga attactcttc gctaaaccag
     3001 tcgctgtttt cctggcggat ctggcgcacg gattcgctga tttcctgcaa atgttgcgtc
     3061 attgcacgtt ctaccgcatc gccatcacgt ttttgcaggg cagagaaaat atcaagatgc
     3121 tggcgtaaca gcatttctgg tggagaaaca tggtcgaaac tcatatagcg cacgcgatca
     3181 acggtcgctt tcaggttctc aatggtatcc cacgccagtt gacagtcggc aatctgcgtc
     3241 aggagttgat ggaagttgtc atcaagttca aaaaaatcat ccagttgctt gcgctcaatg
     3301 gcaatgcgtt gctggtgaag attttgttcc agttggtagc actggctttc ggtaatcatg
     3361 ctcgccgccc gacgcgccac cgcgcactca atggcctgac ggataaaact gccgttgcgc
     3421 acctgggcca tggaaatttt gttgacgtag ctgccacgct gcggacgaat ttgaatcagg
     3481 ccgttttccg ccagtttaat aaaggcttca cgaaccggct ggcgtgacac attgaaacga
     3541 acagaaactt ctttttccga caacggtgtg cctggtgcaa tcaggcaatg gacaatgtcg
     3601 cgacgaagaa tacgataaat ctgctgattg acaggctgtg tgggattaag ttgcgtttcg
     3661 acggtcattc gttcttgctt gtgagtgagt taactgcatg agtctaccac ttttttgtga
     3721 caagcaataa cccggcagtt acgccgggta taaaaattac tgacggtgga cattcagtcc
     3781 ggcatagctt tgggtaaccg gcatcatttc cagggtattg atattgacgt gagcaggcag
     3841 cgttgacacc caccagacgg cttcgctgac atcttctggc gtcaatgcaa cggtattttg
     3901 ataggttttt tccgctttac cgtcatcgcc tttaaagcgg acattggaaa actcggtgcc
     3961 acccaccaga cccggttcga tgtcggtgac gcgcaccgcc gtaccatgca gatccgtacg
     4021 cagattcagg ctaaactgac gaacaaacgc tttcgtcgca ccgtaaacgt taccaccggc
     4081 atacggccag ctgcctgccg ttgagccaat gttaataata tgaccatgat tacgttcaac
     4141 catacccggt aagacggcgc gcgtcatata tactaggcct ttgttgttgg tatcaatcat
     4201 cgtttcccag tcttcaacgc tggctttatg cgcaggctcc atgcccaacg ccaggccggc
     4261 attatttacc aggatatcaa tattgctcca ctcggcaggg agcgatgcca gcatctcttc
     4321 aatagcggcg cggttgcgaa cgtccagttg ggcgatatac agattatctc ccagttcgtc
     4381 tgttaactcc tgcaaccgct cctggcgacg gccagtggcg ataactttat gcccttgttg
     4441 aataaaacga cgagtaatgc attcaccaaa acctgccgtt gctccagtta ctaaaacgat
     4501 catctcactg ttcctcaacg cttttgtgtg tcaataacat agcacgtgat gcttaagagc
     4561 gggtaatact ggtttgcaag attgcactcc gttatcagta agcctacgct atggaacacc
     4621 tcgcgttcag gagaagaaaa tgacaacaat gaatcctttc cttgtgcaaa gcacactgcc
     4681 gtatctggct cctcattttg atcaaattgc caatcatcac tatcgcccgg cattcgatga
     4741 gggaatgcaa caaaagcggg cagaaattgc tgccatcgcg cttaacccgc aaacgcctga
     4801 tttcaacaat actattctgg cactggaaca aagcggagaa ttaattaccc gcgttaccag
     4861 cgtctttttt gcgatgactg cggcgcatac caatgatgaa ttacagcgtc ttgatgaaca
     4921 gttttccgct gaactggcgg aactggctaa tgatatctat ctgaacggtg aattattcgc
     4981 gcgggtagat gctgtctggc agcgccgtga atccctgggg cttgatagtg aatccatccg
     5041 cctggtggag gttattcatc aacgttttgt ccttgccgga gccaaacttg cacaagctga
     5101 taaagcaaaa ttaaaagtac tgaatacaga agctgcgacc ctgaccagcc agtttaacca
     5161 gcggttactt gcagcaaata aatccggcgg tctggttgtg aacgatatcg cgcagctggc
     5221 aggaatgagt gagcaagaga ttgcgctggc ggcagaggcg gctcgcgaga aaggtctgga
     5281 taacaaatgg ctgattccgc tgctgaatac cacccaacaa ccggcgcttg ccgaaatgcg
     5341 cgatcgtgcg acgcgtgaaa aactgtttat tgcgggctgg acgcgagcgg aaaaaaatga
     5401 tgccaatgat acccgcgcta tcattcaacg tctggtagag attcgcgcac agcaggcgaa
     5461 actgcttggt tttcctcatt atgccgcatg gaaaatcgcc gatcagatgg caaaaacgcc
     5521 agaagcagca ctcaacttta tgcgggaaat tgttccagcg gcgcgtcaac gtgcgagcga
     5581 tgaattagcc tccatacagg cggttatcga taagcagcag ggcgggttta gcgcgcagcc
     5641 gtgggactgg gcattttatg cggaacaggt acggcgggag aaatttgatc ttgatgaggc
     5701 gcagctcaag ccatattttg aattaaacac ggtgttaaat gaaggtgtat tctggaccgc
     5761 gaatcagctc ttcggtatta agtttgtcga acgttttgat attccggtct accatcctga
     5821 cgttcgtgtg tgggaaattt ttgatcataa tggcgtggga ctggcgttat tttacggtga
     5881 tttcttcgcc cgtgattcaa aaagcggcgg tgcatggatg ggcaattttg ttgagcaatc
     5941 aacgcttaat gaaacgcatc cggtaattta taacgtctgc aattatcaga aacccgctgc
     6001 cggtgagcct gcgttgttac tctgggatga tgtaataacc ttattccatg aatttggtca
     6061 tacgctgcac ggcctttttg cccgccagcg ttatgccacg ctttccggca ccaacacgcc
     6121 gcgtgatttt gtcgaatttc cgtcgcaaat caacgaacac tgggcaacgc atccgcaggt
     6181 attcgctcgc tacgcccggc attatcagag cggggcagca atgcctgacg aactgcaaca
     6241 gaaaatgcgt aatgccagcc tgttcaacaa agggtatgag atgagcgaac tgcttagcgc
     6301 cgcacttctc gatatgcgct ggcattgcct ggaagaaaac gaagcaatgc aggatgtcga
     6361 tgattttgaa ttgcgggcgc tggtggcgga aaatatggat cttcctgcta taccgccacg
     6421 ctatcgcagc agttatttcg cccatatttt tggtggcgga tatgctgcag gttattacgc
     6481 ttatctgtgg acgcaaatgt tggccgatga tggttaccag tggtttgttg agcagggcgg
     6541 attaacgcgt gaaaatgggc agcgttttcg cgaggcgatc ctttccagag gtaacagcga
     6601 agatctggaa cgcctgcatc gacaatggcg tggtaaggct cctcagatta tgccgatgct
     6661 gcaacatcgt ggcttgaaca tataaacacc accaatggct ggcctggaga tatcgccagg
     6721 ccagcctggt tatagcagta attgcagcag ctgggcgagg gcaaacctca ccgctaaagc
     6781 taataccgtt tcgcagtcgc cagcaaaatg cataaccgca gtgtagttct ggcctttaat
     6841 atgccacgcg aaccagacgg tacccgctgg cgtaccatct tcaccgccct ccggtccgcc
     6901 gtagccggta atggcaatac tgacatcagc atccgcacgc tctatggcac ctgttgccat
     6961 ttctgctgcc actttctcac tcaccgcaga atatcgttca agagactgct ggcttacgct
     7021 gaggattttc atctttgcct gatcggtgaa agtaacaaag cctgcaccgt aaaatttggg
     7081 tgtatcttca gctgcacaca gggcgctagc caacttaccg ccggtgcatg attcggcggt
     7141 agttaagcgt aattgatccg cgataagtct ttgactcaac gcggatgtca ggttttcaat
     7201 agtgtcggta tctgcgagtt gtacaatttt atctttatta atgttcactg ttttttgtcc
     7261 acgctattat atgtaaaggt gatttgcttt ttctgtatgc tcgtccaata taataatttt
     7321 caaacatata aatccgatta ttctgaaaac atgtttaaga ctcttcaatc ttcttaactt
     7381 tagtgagtca aattctctga atatttgaca gctagtgggc tgattttaaa tgccttataa
     7441 caattggcag tattcttatt tggcaattat aaaaatagca accagactta ataagtcaaa
     7501 ccaaacgcaa ttaaggagtt agtatgaaat ttcagtccat tgtattagca agttttcttg
     7561 tcatgcctta tgcgttggca gatgaccagg gcggtttaaa acaggatgca gcgccaccgc
     7621 cgcctcatgc aatagaagat ggctatcgcg gaaccgatga tgcaaaaaaa atgaccgttg
     7681 atttcgcaaa aaacatgcac gatggcgctt cggtttcact acgcggtaat ttgatttctc
     7741 acaaag
//
LOCUS       DAZSIO010000167         7713 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZSIO010000167 DAZSIO010000000
VERSION     DAZSIO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.169"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            106..324
                     /locus_tag="ACHX5T_003415"
     CDS             106..324
                     /locus_tag="ACHX5T_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFR8468468.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            542..784
                     /gene="yiiF"
                     /locus_tag="ACHX5T_003416"
     CDS             542..784
                     /gene="yiiF"
                     /locus_tag="ACHX5T_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR8468469.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1114..2043)
                     /gene="fdhE"
                     /locus_tag="ACHX5T_003417"
     CDS             complement(1114..2043)
                     /gene="fdhE"
                     /locus_tag="ACHX5T_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR8468470.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2040..2675)
                     /gene="fdoI"
                     /locus_tag="ACHX5T_003418"
     CDS             complement(2040..2675)
                     /gene="fdoI"
                     /locus_tag="ACHX5T_003418"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR8468471.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2672..3574)
                     /gene="fdoH"
                     /locus_tag="ACHX5T_003419"
     CDS             complement(2672..3574)
                     /gene="fdoH"
                     /locus_tag="ACHX5T_003419"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR8468472.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3587..6637)
                     /gene="fdnG"
                     /locus_tag="ACHX5T_003420"
     CDS             complement(3587..6637)
                     /gene="fdnG"
                     /locus_tag="ACHX5T_003420"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(6050..6052),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR8468473.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6831..7664
                     /gene="fdhD"
                     /locus_tag="ACHX5T_003421"
     CDS             6831..7664
                     /gene="fdhD"
                     /locus_tag="ACHX5T_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR8468474.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHVAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1773 a         1992 c         2063 g         1885 t
ORIGIN      
        1 cctcgcaaaa acaaaatgac atgcaaaaat ccgcattttt ttaaatgctt ttccggcgta
       61 attttcgcac atacattaat gagtaatata tgtaaggtgg tggtaatggc gatgaatacg
      121 gtttttcttc atttatcaga agaggcaatt aaacggctga acaagcttcg gggctggcgt
      181 aaggtttcac gatctgcaat tttacgcgaa gcggttgagc aatatctgga gcggcagcaa
      241 tttccggtgc gtaaagcaaa gggaggcagg caaaaggggg aggtagttgg tgttgatgat
      301 cagtgtaagg agcataagga atgaaaaaaa tttttcattc ttgaatataa aaaacagatg
      361 cccttattct ggtattaata caaggctgtt ttacttgaac ttataataac tgcaactgtt
      421 acatcgtatc tggaaaacgc ctcgcaaaaa taaaaaatga tgcgtaaatg agctttcttt
      481 aaagattcgg caagggtact tttctctcca tagattcaaa aatgatattg gcgagatatt
      541 tatgaactct ctggcaggta tagatatggg cagaatttta ctcgatttat cgaatgaggt
      601 gattaagcaa cttgatgatc tcgaggtgca gcgtaatctt cctcgtgcgg acctattaag
      661 ggaagcggta gatcaatacc tgataaatca atcgcaaaca gcaagaacca gtgttcctgg
      721 catctggcaa gggtgtgagg aagatggtgt cgaatatcag cgtaagctgc gcgaggaatg
      781 gtaatgggaa aaaatgatgt caatcagatt gctgacaacg tgcgcgttgt tcatgccgga
      841 tgcggcgtga acgccttatc cggcctacaa agtcatgcaa attcaatata ttgaagttta
      901 ttggtaggcc tgataagcgc agcgcatcag gcaatcatac gcttgtcatc agtgacatac
      961 ccataacctt atcgatgtgt tggagggaag aacgaggcaa acagacaatt gcggcatgat
     1021 cttgaatggg taacgcggaa tacgagagat ggtgtcgtca caccctatga gctgtaatcc
     1081 gggcgacctg cgccacccgg aaaacgtcag agattactct ccttcacccg gaaacaggaa
     1141 cgggttaatg gaactacggg cgtagccttc ttgctccatt cgcgcgtcca gtaccagaga
     1201 ggcgaggtca tctgccacgg cttcaacttt cggttctttt tcctgataaa gaatcttcag
     1261 gtaagtgccg cagtcatcgc agctttcggc tttaatcgcg gcctgttcgt catccagcga
     1321 ccagtaatgc agtttgccgc tctgctcgca gttgctgcat tttacgcgca ctacgtgcca
     1381 ttcggtttca cacaggttgc agtgcaggta acgcagaccc tgagtggtgc caatttgcac
     1441 catgctggac accggcatag agccacatac cgggcaatat tgacgttgtt cgccgtattc
     1501 agcgcgggct ttgccgggga tcagattggc catctgcgcc cagtagagcg acagcgcagc
     1561 ccagataaac ggcgctttat cgctgctgac ggacgagaaa tcagaggcaa acagcgcgct
     1621 ggccatatct tccagctcct gagtcgatgc cttctccaga ttctcaatca ctgccagcgc
     1681 cgggccgctc atttcaggtt tcagctcagc aattagcgcc atcagcagct tttgccagtg
     1741 cttatcacgc ggcagaacgt gaatatccag cgggggcttg ccctgtgcgc tggcttcttt
     1801 aatgcgcgcg gtcagatcca tctccagcgg atggtcgtac agcaccactt tctgggcgtg
     1861 ggcgataagc gcagcaaagc gcaggtaatc acccagcgga ttattttctg ccagctcgcg
     1921 cagacgctcg gcgcggcggt tgtataaatt cttgagccga gggaacaata acggcggaat
     1981 catatccgcc gtacgtttct cgctcgaacc cagctcatct tgcgggatta tgcgaatact
     2041 cattcagctt tcttttccgt tgtcttgcgg acctcacggt accagcgcgg gtgatgtttc
     2101 ttcgcccagg cgctggtaac ccatccttcc accatggcgg taatcgtgcc tttcacccaa
     2161 agggcggcgt agatatgcac catgataacc acaattaacg ccactgcggc aaatgaatgc
     2221 agcattaacg cgaatcggat caccgggatt gagaaagcag gcgcaaaata aggacgccag
     2281 ataatcacgc cgctcaccag cagcagaacc aggaaaataa tcgccgccca gaaaacgcat
     2341 ttctgaccga agttataacg cccggtgtca cctacttcct cgttgacgac gatcttacga
     2401 atattcttcg cccaaaagat atcatcccga ttgattaggt tttgatgcca gtaacggaaa
     2461 aacatgatga tgaacgaggc aaacataacc acgccgacaa acgggtgcag aattcgcgcc
     2521 agctgcggtg tgcccatgat ttgcatcaac cagttgaagg acgggaacaa aaagcccagc
     2581 ccgctcaccg ccgccaggat gaagcagaag gcggtgatcc agtggttgat acgttccggc
     2641 gcggtgtagc gcacgatggt gtcacgtcgt ttcatttgcg ctcctcgtct ttctcttcgt
     2701 gcagattatt ctcttcctca tccgcacggt tcggaccgac acccacgtag tggaagatac
     2761 tggccgcaaa ggtagccgca aagccaacag ccgcgagcgg tttccagatg cctttccaga
     2821 atttcacggt ttcgctgatt tccgggttct ccggcaagcc atgatacaga tttggcttgt
     2881 cagcatggtg cagcacgtac atgacgtgtg taccaccgac gcctgccgga tcgtacagac
     2941 ccgcattgtc gtaaccgcgg gttttcagct cagcaacgcg ctcgctcgcc agcgttttca
     3001 tcgactcttt cgtgccgaag tgaatcgcgc ccgttgggca ggtcttcacg caggctggtt
     3061 cttgcccaac caccacgcgg tcaacgcaca gcgtacattt gtagacgcgg ttgtcttccg
     3121 ggttgaggcg cggaatgtcg aacggacagc ccgcaatgca ataaccgcag ccaatgcact
     3181 gctcggactg gaagtcgacg ataccgttgg catactgaat aattgcacct tccgccgggc
     3241 acgctttcag gcagcctgga tcggaacagt gcatacagcc gtctttgcgg atcagccatt
     3301 ccagtttgtc gttctgctcc acttccgaga agcgcattac cgtccacgat ttggcgctta
     3361 aatcattggg gttgtcgtac accccaatgt tattgccgac ggtatcgcga atgtcgttcc
     3421 actctgaaca cgccacctga caggctttac agccgataca ggtggtaacg tcgatgagtt
     3481 tcgccacttc ttcctggaag tcccgcgcct gaggcgcggg ggtcagaccg ttagtcgcgg
     3541 aacgacgaat gatatcttga gattgataag ccatatgtcg tctccgttac accttttcca
     3601 cattcacaag gaaggactta aactccggcg tctgcgtgtt cgcatcacca acgaatggcg
     3661 ttaaagtatt ggcaataaag ccttttttag ctacgccttc atagccccag tgaatcggaa
     3721 taccgatggt atcgatatct ttgccgttcg ctttcagcgt gcgaatacgt ttggtcacca
     3781 ccgctttggc tttgatatag ccacggttgg aggagacttt cacggtatcg ccctgggcaa
     3841 tgccaagttt attcgccagc gactccccga tttccacaaa ctgctctggc tgcaaaatcg
     3901 cgttcaacag cgcgtgtttg gtccagtagt ggaagtgctc ggtcagacga taggtggttc
     3961 cgacatacgg gaacttatcg gctttaccca atgcttcggc gtcgtcttta aagatacgtg
     4021 cagctgggtt cgagataacg tttggatgca gcgggttagt tcccagcggc gtttcaaacg
     4081 gctcgtagtg ttccgggaac ggaccttccg ccatcttatc gagggcaaac agacgcccca
     4141 tgccttcctg ctgcatgata aacggcccga cgccgctacc cggaggcgct gcgctgtagt
     4201 ccggaatatc ccagccggtc cacttagtgc cgtcccattt cagcaactga cgcttcggat
     4261 cccacgggtt accctgcgga tctgcggagg cgcggttata cagaatgcgg cggttaagcg
     4321 gccatgccca tgcccagccc agcgtgttac cgaggccaga cggatcggcg ttatcacgac
     4381 gcgccatctg gttgccttcc ggcgtccagc taccggcgaa aatccagcag ccacaggagg
     4441 ttgtaccgtc atcgcgcaat tgggcgaacg agctaagttg ttggcctttc ttgacgataa
     4501 ccgcaccggt tgccggatcg gtaatatcgg ccagcgcctt accgttgctc tccatcgcta
     4561 cttcttccga cttcggctca tgcggaatgg cgtagttcca ggtcatgttc agcacctgat
     4621 ccgggttcgc gccaccctgt tcggcataca tcttgcgcaa gcgcaggaag ataccggaga
     4681 ggatctcacc gtcagtcagc gcaatccccg gggcgtccgc acctttccag tgccactgca
     4741 accagcgacc tgagttaacg attgaaccgt tctcttctgc gaagcaggtc gatggcagac
     4801 ggaacacttc ggtctggatc ttcgacgagt caacttcgtt cagctcaccg tggttctgcc
     4861 agaagttaga ggtttcagtg ttcagtgggt cgatggtgac gaggaacttc agtttcgaca
     4921 gacaaccgat cactttgttt ttgttcggga atgaggcaac agggttaaag ccctggcaga
     4981 tatagccatt gaccttgccc tctttcatca tctcgaagta ttgcaggacg tcgtagcctt
     5041 tatcccactt cggcaaccag tcaaagcccc agctattttc cgccgtcgct ttatcaccaa
     5101 agaaggcctt catcatagag acgaagaatt tcgggtagtt gccccagtag tttacctggc
     5161 cttccagcag cggttttggc gtgttggcgg taaggtaggt ttgcagatcg gtctgcttct
     5221 cgcttggcag cgtcatgtaa cctggcagac tctgcgacag tagccccagg tccgtcagcc
     5281 cctgaatatt ggagtgaccg cgcagggcgt taacgccgcc gccggccatc cccatattgc
     5341 cgagcagcag ctggatcatc gccatcgtac gaatgttttg cgcaccaacg gagtgttgcg
     5401 tccagccgag ggcatacagg aacgaggcag ttttatcgtg agcactggtt tctgcgatgt
     5461 attcgcagac tttcaggaac gcgtcttttg gcgtaccaca gatgttttca accacatctg
     5521 gcgtgtagcg ggaaacgtgc tgtttcagca agttccacac gcagcgcgga tgttgcagcg
     5581 tggtatcgcg tttggcgaag ccgttttcgt ccagttcata agtccaggag gatttatcgt
     5641 acttgcgttt ttccgcgtcg tagccggtga acaggccatc ttcaaagccg taatcctcac
     5701 gcacgatcag gctggcgttg gtataggctt cggtgtattc gtggttgaat ttttcattgt
     5761 tcagcaggta cagcaatacg cctgacagga aagcaatgtc agtaccggaa cgaatagggg
     5821 catagtagtc agctaccgcc gccgtacgcg taaagcgagg atcgatcaca atcagcttcg
     5881 cgccgttgtg aattttggct tccatcgccc agcggaaccc gaccgggtga gcttcagcgg
     5941 cgttaccgcc catcaccacg acgaggttgg cgttcttgat gtcgacccag tggttggtca
     6001 tcgcaccgcg accaaatgtt ggagcaagac ttgctaccgt tggtccgtgt cagacacgcg
     6061 cctggttgtc gaccgcgagc atacccagcg cgcgggagaa tttttgcgtt aaatagccgg
     6121 tttcgttact cgacgcggaa gcacacagca tcccggtgga gagccagcgg ttaacagtca
     6181 cgccttcggc gttttgcgca atgtagttag catcgcggtc ttctttcatc agtttggcga
     6241 tgcgatcaaa cgcctcttcc caactgattt gctgccattt atcagaacct ggcgcacggt
     6301 attccggaaa cttcagacgg ctttcggagt ggatgaaatc caccaggcca gcgcctttcg
     6361 gacaaagtgc accgcggttg accgggtgat ccggatcgcc ttcgatatgg aagatagatg
     6421 ctttggcgtt ttttgctccg tcaccgaggc tgtacatcaa cagcccacag cctacggaac
     6481 aataggtgca ggtattacgg gtttcgcggg tgcgcagcag tttatactgc cgggtttccg
     6541 cgagtgctac gctgggtgca aaacccagtg ccgctgccgt ggtgcctgcc ataccgccag
     6601 cgcagatctt aaagaactgc cttctgctga cctgcatgga ttgctccttg tttcgacatt
     6661 gtcacgtccc atttacattc gcttgctgcg tgtgcaggga gtgggagtta tttttctttg
     6721 cggaaggggc cgcaaaggtc cagaattggc tcaatttccc tccatctagg aagggtttgt
     6781 aacagaatac cataatgttg gtgtgtatgt tcttatctgg ttaagagaaa gtgaaaaaaa
     6841 cacagcgaaa agaaatcgaa aatgtgacaa atatcacagg tgttcgtcaa attgagttat
     6901 ggcggcgtga tgatttacaa cacccacggt tagatgaggt cgcggaagaa gttcccgttg
     6961 cgctggtcta caacggcatt tcgcatgtgg tgatgatggc gtcgcccaaa gaccttgagt
     7021 actttgcgct cggtttttcg ctttccgagg ggataataga aagtccgcgc gatatcttcg
     7081 gcatggatgt cgttccttcc tgtaatggtc ttgaagtaca aattgagctt tccagccgcc
     7141 gctttatggg gttgaaggag cgccgccggg cgctggcggg acgtacgggc tgtggcgtat
     7201 gcggcgtgga gcaacttaat gacatcggaa aaccggtgca gccgctaccg ttcacccaga
     7261 cgtttgatct caacaaactg gatgatgcat tacgtcatct caacgatttt cagccagtgg
     7321 ggcgactgac tggttgtact cacgtcgctg cctggatgtt gccatctggc gaactggtcg
     7381 gcgggcatga agacgtgggt cgccatgtgg cgctggacaa actgttaggc cgccggtcac
     7441 aagaagggga aagctggcag caaggtgcgg tactggtttc cagtcgtgcc agttatgaaa
     7501 tggtgcaaaa gtcggcgatg tgcggcgtag agattttgtt tgcggtgtct gccgcgacca
     7561 cgcttgctgt agaagtggct gagcgctgta atctgacact ggtaggtttt tgtaaaccgg
     7621 gtcgggcaac ggtttatacc catccgcagc gtttgagcaa ttaattaaaa taagtcctgc
     7681 gatatatatt aaattgcagg acttattcat ttc
//
LOCUS       DAZSIO010000168         7684 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZSIO010000168 DAZSIO010000000
VERSION     DAZSIO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.170"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(31..106)
                     /locus_tag="ACHX5T_003422"
     tRNA            complement(31..106)
                     /locus_tag="ACHX5T_003422"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71..73),aa:Asn,seq:gtt)
     gene            complement(207..1678)
                     /gene="yeeO"
                     /locus_tag="ACHX5T_003423"
                     /pseudo
     CDS             complement(207..1678)
                     /gene="yeeO"
                     /locus_tag="ACHX5T_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            1839..1914
                     /locus_tag="ACHX5T_003424"
     tRNA            1839..1914
                     /locus_tag="ACHX5T_003424"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1872..1874,aa:Asn,seq:gtt)
     gene            complement(1952..2902)
                     /gene="cbl"
                     /locus_tag="ACHX5T_003425"
     CDS             complement(1952..2902)
                     /gene="cbl"
                     /locus_tag="ACHX5T_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR8468475.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(3004..3921)
                     /gene="nac"
                     /locus_tag="ACHX5T_003426"
     CDS             complement(3004..3921)
                     /gene="nac"
                     /locus_tag="ACHX5T_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR8468476.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4249..4324
                     /locus_tag="ACHX5T_003427"
     tRNA            4249..4324
                     /locus_tag="ACHX5T_003427"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4282..4284,aa:Asn,seq:gtt)
     gene            complement(4380..5315)
                     /gene="ldtA"
                     /locus_tag="ACHX5T_003428"
     CDS             complement(4380..5315)
                     /gene="ldtA"
                     /locus_tag="ACHX5T_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR8468477.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5377..6456)
                     /gene="cobT"
                     /locus_tag="ACHX5T_003429"
     CDS             complement(5377..6456)
                     /gene="cobT"
                     /locus_tag="ACHX5T_003429"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR8468478.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6468..7211)
                     /gene="cobS"
                     /locus_tag="ACHX5T_003430"
     CDS             complement(6468..7211)
                     /gene="cobS"
                     /locus_tag="ACHX5T_003430"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR8468479.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7208..>7684)
                     /gene="cobU"
                     /locus_tag="ACHX5T_003431"
     CDS             complement(7208..>7684)
                     /gene="cobU"
                     /locus_tag="ACHX5T_003431"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFR8468480.1"
                     /translation="SSQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDEL
                     IHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQR
                     CPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2123 a         2077 c         1665 g         1819 t
ORIGIN      
        1 gtctcataga gatgtctcaa aactaaaatt tggctcctct gactggactc gaaccagtga
       61 catacggatt aacagtccgc cgttctaccg actgaactac agaggaatcg tgagaacgag
      121 gcgaatatta gcgatgccca cccacaatgt caaagcctgt tttttaaatt tgaaatcgtt
      181 tgctgaaata atctgcattt tgtcgtttat tccgacacaa ctggcttttt ttcacacttt
      241 tgcggctcgg atcgagggta tttccacagc caacgtccag taaccattcg ccagtaaaac
      301 agcacggccc gcacagccca gtcggcaaac attcccatcc agacaccaac cacaccccag
      361 ccaagcatga ttccctgcac ataaccgact acaacccggc aaccccacat gctcaacata
      421 gaaacccaca tggcgtaacg ggcatcacga gcacccttaa atccagcggg tagcacccat
      481 gaggcggacc aaataggcat aaataaagca tttagctaaa tcagaatcac aacgacgtgt
      541 ttaacctgtg gatcctgggt gtaaaacgat gccataaccc cggcaaaggg agccgttagc
      601 caggcgatgg ccgttaatcc aagagtggac agccagaaca catgccgcaa ctgaatctct
      661 gcttgcgcta tctgccctac ccccaacctt cggcctgtaa tgatcgtaga agcagaaccg
      721 agcgcacttc cgggtaagtt gataagagcc gcaattgaga acgcgataaa atttccggca
      781 ataacgctgg tccccatccc ggcaacgaac atttgggtta ataaccgacc actggtaaat
      841 aacactgatt cgacactcgc tggaatacca atccccatga cttcccagat aatgctaaaa
      901 ttcagcggtt taaaatagct ctttaacgaa atccttagcg caggattaaa accaatcgcc
      961 agcacccaca aaattgcaac tgcgccaata taacgagaaa tggttaaacc caacccagcc
     1021 ccgaccagga gaaaaggccg taaatcaata tgccgctaat aataatatta agaatattca
     1081 ggctaccgtt aatcagtagc ggtattttcg tattccctgc accacgaagt gccccgctac
     1141 caataagagt gatggcagcg gctggataac tgagtacggt cagctccaga taagtcaacg
     1201 ccagtgcttt aacttctgtc gtggcatcac ccgcgacgaa atcaataatt tgttcgccaa
     1261 aatgatgaat tagcgttgcc aacagtacgg caaacaacgt catgatcacc aatgactgcc
     1321 gcgtcgccac cctcgctcgt cgtcgatccc gcttaccgag actaaatgct acaacgacag
     1381 tagtaccaag atcgatagct gcaaaaaaag ccataatgac catattgaag ctgtccgcca
     1441 accccacgcc ggccatcgca tcttttccca gccaactgac cagaaaagtg ctcaggaccc
     1501 ccatcaacag gacacaggcg ttctccatga agataggaac agcaagcggg gttatctcgc
     1561 gctagaacaa cactttgtag ctcttgcgtt tagagtgcca gcgagtgccg tgaacaacct
     1621 ggcgtaaagc agaggagata ttcaaagccg accttaattg cagaaagtga aaccacattt
     1681 caaatgatga gggagaatca gcaaagctgc aaagattttc gccaacaaat tgtctgcaaa
     1741 tgcaacaaac tgttgataga aacggcaaac agttgggaaa tttaaaaatc gggtttgaca
     1801 aaatattttt cgccgttaag atgtgcctca acagcgattc ctctgtagtt cagtcggtag
     1861 aacggcggac tgttaatccg tatgtcactg gttcgagtcc agtcagagga gccaaatttc
     1921 tgaaaagccc gcttttatag cgggcttttt gctatatctg ataatcaatt tcctcttcac
     1981 tgttttccat cacctggcgc ttgatatcct caactgacag tcctgcatta caaagttcca
     2041 gaaagcgcca aacatagtta cgctgaagtt gtcctcgctt cagtcccaac cagacagtat
     2101 tagcatcgaa aagatgccgc gtatccaggc ggattaaatt ctcttcctct tgttcgccgc
     2161 tggattgttc ggcaactaat ccgatcccaa gcccaagagc aacataggtt ttaatgacat
     2221 cagaatcctg tgcacttaat acaatatctg ccggcaaacc tttgcgggta aatgcgtcat
     2281 cagtgcgtga gcgccccgta attccctgtc ggtaagtaat taacggccac ttcgctattg
     2341 attccagcgt caatggtgta atttgcgtta agggatgatc gagtggaaca agcaaactat
     2401 ggtgccaaca aaaccatggg aaggcgacga gctgcgggtc attactcaaa cgctcgctgg
     2461 cgataccaat atcagcttcg ccattttgca acaacgtcgc aatttcctgt ggcgaaccct
     2521 ggattagctc gagccgaacc tccgggaaaa gttcgcgaaa agctttaatg acctctggca
     2581 agctataacg tgcctgagta tgcgtcgttg caatagtgag aacaccagac gtatcgttgg
     2641 taaacaggtc tgcaagccga cgaacattac tggcttcatt cagaatacgt tctgcaatga
     2701 ccagtaatgc tttgcccggt tcagtcatcc ccagcagtcg cttacctcgt cgaacaaata
     2761 tttcgatgcc aaattcatcc tccagttccc gaatatgacg gctaacgcct gactgtgagg
     2821 taaaaagcat attcgcaacc tctgtcaggt tgtaatcctg acgtgcagcc tcgcggatta
     2881 tctttagttg ttggaaattc acggtaaact ccgggcagtt cagatatccc gttattgtta
     2941 aagtctaatg cccggcataa caaataataa aaacccgcat cttattccat cccgatataa
     3001 cgcttagctc accaattgcc actgcctttt ttccatcact ggagaactaa tcactgacat
     3061 taacaactct ttcactgcct gtgcctgtgg tgacaagttt gctctggcgg gtaaatttaa
     3121 ggacaaagag agactcattg aaggagtggt aatgcgtgac atccacccat ttactgcgcc
     3181 acataacgaa cgcgcagccg attcaggtaa tactgcaacg cccatgccgc tggcaatcgc
     3241 tgcggtaagc gtggcaatag actcaatttc accaatgact tttgccgtga gccgccgtag
     3301 ggaaaaagcc tcatcaacac gaagtctaac agcgctgtaa tcactgggga gaaaaaggtt
     3361 catttgcgca atagcattaa catcaacgtt ttgccccggg caatcttgag ttcctaccag
     3421 gaaaagatct tctttcagca aagcctgact ggatacccca gccacagggg aatgctcata
     3481 aatcaccgcc atatcgagtt ggtgatttat cagtttttcg ttaagcactg caccactatt
     3541 ttcatgaaga tagataacga cctccgggaa ttcagcgcga accgcctgta ataagggcat
     3601 ggtgatggat gacgcagcgg ttcctggcgc aaagccaatc gagacttgcc ccgataatga
     3661 ctgaccaacg ttatgcaccg ccagttgggc ctgctcacac tgacgtaaaa tggcccgtgc
     3721 atgggtatag agaatttttc cggcgtctgt tggcgtaacg ccccgttttg tgcgaatcaa
     3781 aagctgttga tttaactcac cttccagtat ggcaacctgt tggctgagcg ctggttgtgc
     3841 gatatgcaat acttcagcag cctgggtcag gctaccaata tctacaattt ttacgaagta
     3901 tttcaggcgt ctgaagttca tgttgcctcc ggtttttaag aatcggccca agtgccgcca
     3961 ttacttacaa ccagtatgca agatgcctgc cagttctatt ttggcgttga tgcgcaagct
     4021 aaccaactgt caaataagag attatgatag attcgtcatt ggcccttttt atcagcagtc
     4081 gcattcacct gccctataaa aggagggtat gcaccacgat ggttcattac ccaataagat
     4141 tgaaagctca ccactttgtt gaaattggca gcaaacaaac aaaaaaaatg catttcaccc
     4201 tttgacatca ccatgcactg ccattaatat gcgccccgtt cacacgattc ctctgtagtt
     4261 cagtcggtag aacggcggac tgttaatccg tatgtcactg gttcgagtcc agtcagagga
     4321 gccaaattca aaaaagcctg ctttcgagca ggctttttac tttctaatta ccaacgctct
     4381 taaaacatct gtcttgaacc agaactaatt tgcacaggca ttcccgatcg atgttgcaac
     4441 gcagcatttg cgcgatttac atcaacttct tgcccgttga taaacgcccg caaagatggg
     4501 gttaccggca atggcacttt tcggtcagac tcatattctg cacgattgcg cgacagcggc
     4561 tcatgaactt ccagccactt tgaaccatcc ggttccgttg tgtatttcac tggctgatca
     4621 ataatttgta cacgagtgcc tacaggaaca ttatcaaaca gatatttgat atcgtcatta
     4681 cgcagacgaa tacagccctg acttacacgg agcccaatac caaaattggc attggtaccg
     4741 tggatggcat acaacctgcc aatataaatc gcgtacagcc ccatggggtt atcgggcccc
     4801 gcaggaacaa atgcgggcaa actctcccct cgtttcgcat attcgcgccg gatgttcggc
     4861 gttggcgtcc aggttggtgc ttcttgttta cgttcaacgg tagtcaccca gttacgcggg
     4921 gtttctcgcc cagcctggcc gataccaata ggaaagactt ccactgtatt actgtctggc
     4981 ggatagtaat aaagacgcat ttcagcgacg ttaaccacaa tccctttacg aacagtggcg
     5041 ggcaaaatca gttgctgcgg aatggtgagt tgtgagccag gcttcggcaa aaaaacatca
     5101 gcgcccggat tcgcttccag catattactt aacccttgcc cgtattgtgc ggcaaaagtc
     5161 tccagcggct gagtattgtg atcaggaaca gttacagtaa gcgactgccc cactaaacgg
     5221 ctaccctctg gaggtaatgg ataagttacc gccaggctat tttggctggc aaaaagcaga
     5281 gcaaatgagc aaagaatatt tacacgacgc atcatgtccc tttcctaagt cgcgaatact
     5341 atccgataag tatagctttt atcagacttt tcgtttttag ctgttcaaat cagaagtcgt
     5401 attccccggt agaacaatat tactggcagc aagttcgccc atgttgttgt atatcgcaca
     5461 agcagcttcg atgatggaca tcgccagagc tgcaccactc ccctcaccta aacgcatatc
     5521 catattgaga taaggctcca gccccaaatg cgagagcgct atacgcgcgc ctttttctgc
     5581 cgacaagtga gaaggaatga gatacggttt gattgcagga gacatctggc aggctgcgag
     5641 cgcagcagca taagaaagaa atccatccag caacacgggt aaaccgcagg aagcagcacc
     5701 taacatcact ccagctattc cgaccaaatc aaatccaccc acttttgcca ggacatcaac
     5761 accatcctga ggatttggtt gattcaacgt aatcgcccga cgcacaacat caattttatt
     5821 agccagttta tctgtcggca ggtttgcgcc aatcccaacc acttcttcag gatcccggcc
     5881 agtgattgtg ctgactattg ccgctgccgg tgtcgtgttt gccatcccca gttcacctac
     5941 accaaacagc gtgacaccgt ttttcgccag ctcccgcgta taacatatga cgtccaaaag
     6001 caacttttca gcctgacggc gactcattgc cggagctgaa gcaatattgc cgctacctcg
     6061 tgcgacacgc atgttgataa gcccggggat aggctcagca gtatcaatac caacatcaat
     6121 tacgtggacg ttagcgcccg cttgtgctgc cagcacacac acgccggttg ttccacgggt
     6181 catattttca gcctgtatgg ctgtcacttc ttttggagaa atagcgaccc cttcctccca
     6241 gacgccgtga tcggcacaca taaccagtac cgcttttttg cccacatgcg gtatgccatt
     6301 cacccccggc attcctgcca gttgtatggc aagcgcctcc agctttccta ggctaccaac
     6361 aggtttgaat aacccgtcaa catgccgttg tgcacgcgac atagcggcag aattgatggc
     6421 tgggatcgta ttcagtaaat cggcaattat ttgcatgtca cgtttcttta tagcagtgcc
     6481 agcaggaaga ccagttcacc aagctctatg gctgctccca gcgtatcgcc cgtttgcccg
     6541 cccagcgtac gtttgagaag ctgaccgagg atgaaaatcg ccaccatcgt caccaccata
     6601 gcagccacac catgcatacc cagcaataat acggcagcaa aaatagcggc taagccgagt
     6661 gtgacgcagg tttgtcgccc atcaattttg ccaatgaaca cattgccaag cccttcttcc
     6721 cgcgcataac gatgacgata catcaataat gcggcagtcc cgcggctgac cgcacatgct
     6781 gccgccagcg atgcaaggat cggttcgcca cgcagagcca actcgcttaa caccagaatc
     6841 tttgccagta ccacaaaaat caatgccaga ccgccgtggg tgcctaaacg gctatcacgc
     6901 atgatttcca acatgcgatc gcgactacgt gcagaaaata cgccatcaca ggtatcagcg
     6961 aggccgtcca gatggaattc cccggtcatc agcgccagca caagtacgct aaacagtgcc
     7021 gccagtggtg cgccacacca tgcctgcagc accatgaaga ccagcccgct aatcgcgcca
     7081 agcaataatc caatcaaagg aaaagtaata ataccgcgag aataatgttc gaaatccagt
     7141 ccctgggacc agcgacgcgg tacaggcaga cgcgtaataa atgagagcat cgcccaaaat
     7201 aatttgctca tttgattttt actccaatac ccgaaaccac cagccatact tcatttgccg
     7261 cagcggccaa ctgctgattt acccgcccgg caatatcacg aaaatgtcgt gccagacgac
     7321 tctccggcac aatccccatt cccacttcgt tagtcactaa tacaaccttt gcggggcaac
     7381 gttggcaggc agcaatcaac gactgaatct cagcattaat cgcctgttcc atcgcctgat
     7441 aatcccattc atcagggtct ttatcgccgc cataatcaaa caacagatta gtcaccattg
     7501 tggtaacgca ttccagcaac acagcctcat tcgggttaat gtctgcatga attaattcat
     7561 caagatgttg ccagcgctcc actgtgcgcc agtgctccgg gcggctttgc cgatgatgtt
     7621 ctatccgtgc agccatctca tcatcaagga tttgcgaggt agcgatatac agaacctgtg
     7681 aaga
//
LOCUS       DAZSIO010000169         7577 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZSIO010000169 DAZSIO010000000
VERSION     DAZSIO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.171"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="ACHX5T_003432"
     CDS             complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="ACHX5T_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HFR8468481.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="ACHX5T_003433"
     CDS             complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="ACHX5T_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR8468482.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="ACHX5T_003434"
     CDS             complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="ACHX5T_003434"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR8468483.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="ACHX5T_003435"
                     /pseudo
     CDS             complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="ACHX5T_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="ACHX5T_003436"
     CDS             complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="ACHX5T_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR8468484.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNCIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="ACHX5T_003437"
     CDS             complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="ACHX5T_003437"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR8468485.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6042..7181)
                     /gene="wza"
                     /locus_tag="ACHX5T_003438"
     CDS             complement(6042..7181)
                     /gene="wza"
                     /locus_tag="ACHX5T_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR8468486.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
BASE COUNT         1762 a         2000 c         1914 g         1901 t
ORIGIN      
        1 acctaaatcg cccagacggg tggaaatata aggaaacgcg attaccacac caacccggaa
       61 tacagccaga gaaatcaatg ctaacggtaa ttttttctta atcgcctctt tattcagata
      121 ttgaaatgcc cactccagca aataaaacag gataaaggtc ataacccctg aaaacgatcc
      181 ggataatatt atccctgcga gaatcagggc atctgtttta ggcgttttga taccaaactg
      241 tttgatgctg agccaaattg agattaatgc cagagcgaaa aatgccggtt cgaaataaag
      301 tgctgtcgtg cgcttgccgc cgaatttaat gaaattcagc acatagctgt tgctgtaaat
      361 cagatatttc gaaatcgact ccataatact gctgccaccg gtgaggataa tttgcgccat
      421 ctccaccgcc gccagcgcca ccaccagtcc gaccaccaga taaaagaaac gtaatatctt
      481 acgatggttg tgtggcgaaa ttgttttaaa acgaatactc cacaccatgc caataatgat
      541 cacaatatag acaaacagca tagttgaagt gacgtattta ccggcatcca acgactgacc
      601 aaacaggtag ttaaacgccg tgagtcccgc gccaatccct aaggcaatca tcaatttttt
      661 aatgctgatg cgttctaaaa acagcagtaa caagacaggt aaaaaagtga cgatggtgat
      721 ggggaaactt tcgccaagct gggcgatttt gacgttaacc agcaggtaga tcagtggcag
      781 cagcaggtag ctacagattc tgatagaagt tgacatactc ctccagcatc tgttgtccac
      841 tgtaggcggc gcgactgcgt tggctgaact cagccagcgt ggtaccaaat atcgcctgcg
      901 cgatttccgg tttgcttaac tgtaccagtt gcagcacctc ttcttcactg acggttttac
      961 caccggattt ttgcagcact tcccgcgccg catcgctatg ggtggcaatc accggcacgc
     1021 caatcgatag cgcctcacac aaaatcagcg ggtagttatc gacacgagaa ctgaacacca
     1081 gcgcatccat ctgattgagc gcgctcatca acttgcgctt gtcagtttca aagccgtgat
     1141 taaccacgtt gccagcggtg aacggcgaga acttaccaaa ggtatgcagt tcgattttgt
     1201 cgcccagcgc catcatctca cgcaccagtt gctggttagt tttgccgtcg taacgcaggt
     1261 catgcgccac cactgcgatt ttcggcttgc cctgagtttc gcgcaccgga ggcagatctg
     1321 ccagaatcgc ttcggttgcc atatcaatgc cattattgat aatccggcaa cgccctggcc
     1381 cgtacagact attgaaagcg tcagccacat gctggctggg ggaaataaac tgacagccca
     1441 gcgccagcat ctcgcggaat aactggcgtt tgcccgccac cagctggtgc gcgcgatcaa
     1501 tcttcaccgg cggatagtta tttaaggtcg ggcatttctg gcagcccgtt ttccagcctt
     1561 cgcaaccgtc ggtaaaggcg cagcgtccgg ttacactcca gtggtcgtgc agcgtccaga
     1621 ccagggtgac atccggttta tggtttttca ctttttcgca aaagcgcacc acgctgttaa
     1681 gattcaacca gtagctgtgc agcacatgaa aatgcaggac caccggaccc ggtgtgcgag
     1741 taatggtgcg atataactca ttgaaattgc cgaacagatc gcgattaaac agacgaaaca
     1801 acgcaatgtt cgccatcgcg gtcatccgcg gcgtatgttt gatgacctgc ggatagttct
     1861 gatggctgac gctctctttg ccgcctttgc cgtagccgta gacaaaatgt gacgccagcc
     1921 cctgttgcag cgcacgctgg tggagatcta acgccacacc tgctgccccg ccttccgcca
     1981 gtcgcacatt aaattgcaga atattcattt aatcaccttc actcgcgctt tttctcccac
     2041 caccagcgcg ttgtccggga cagagtcgag caccacgctg cccgcaccca cggtgacgtt
     2101 gttaccaagc gtgatatcac caagaataat gacgttggca ccgagttcga cgccgttgcc
     2161 aatgtgtgga catgccatgt tatcggcacc ccgattgccg atagtgacgc cgtggcgaat
     2221 ggtgaaatca tcccccgcca ccacgttttt attgatcact acggcgtaac cgtgatggat
     2281 agtaaagcgg cggccaatgg tcgcggcggc ctggatttca taaccgaaaa agcattcggt
     2341 gataatgcga tacagcacca gcagcggggc cgcccacaga ttgttgagga cgttcttttt
     2401 gcgccacacc gaacaaaaat gagcgacgcg ataggcaagt accatgcagc acgggcgtaa
     2461 actccagctg ttggcgcgca gatcttccag catctttagc gcccccgaat accatcagcc
     2521 agacgtttgc cgttacgcac cgacagcagc gtcaacaagg tgcgccaggt catacgttta
     2581 ttgcggatct ggtagagggt aaacagctga tattttttac tggcgcggtc gaatttgtct
     2641 ttgtgcttgc ggtaaaagtg gaagtatccg gagaattttt tcggtgacga ggtgatctgc
     2701 atttcgccgt gattgatatg caggatctgc gtcgcctctt ccactttcca cggttcgccg
     2761 tactccacca ccatccgcag gaatatgtcg taatcctgtg ctgctttcag ttcggtatcg
     2821 aacaaacact ctttgaaacg ccatgcccag gtaaagacct gattgccaat gatattacgt
     2881 ttatagaaca ggcggcgtga gtacggcgat ttgggataca gtggtaagct cgccggttgg
     2941 gaataaactt ctccctagca aacgtagtcg ttagcgtaca aaaaggcgtg tgtgaccagt
     3001 tgctgtttat gggcgaggaa gacgctcagg cggttgggcg tccattcatc atcatcatcg
     3061 atcccggtga tgtattcccc ttgcgccagc ataatcgcct ggttacgcac cgcgcacgcc
     3121 ccgctgttaa tgtcgttgtg gatgtaagtg atacgcggat cgttgagggc agtgacgtac
     3181 tgttgcagtt gttcccagga ggtggagcaa tcatccacga tgatcatctc ccagttgctg
     3241 tagtcctggc gcaggaccga ttttatcgcc cgaatcgcca gttgttggcg gttccaggtt
     3301 ggcatataga ttgagatcag cggattgtct ttcataggtt gttctcccgg cttatgagct
     3361 ggtttgcttg ccggatgcgg cgtgagcgcc ttatccggcc tacggagcgg tgcgaatgca
     3421 ggcgtcgtta tttcgcatcc gacttatatt cgtattcgta atagccataa tcctgatacg
     3481 cgctggcgcg gcggaagatg gagttcagaa tcaccccttt caccggaata cagttttgct
     3541 caaagcggct cagactggtt tccacttctt tcaatgtgtt gaccgcataa cgcgccacca
     3601 ttaacgtggt tccgacatga cgaccaacaa ttgccgcatc ggtcactgcc agaatcggcg
     3661 gcgtatcaat caacaccagg tcatagtttt tactcgccca gttcaccagt tcggcaaagc
     3721 gttcgctcat taacagttca gaaggatttg gcggcacctg accgcgcggg atcaggtcaa
     3781 atttggcaat agaggtcggt ttagcagcgg tagtaatatc gccctgacca atcagaatct
     3841 ccgacagccc attgacatta ttggtgccca acagctcgtg ggtgtagcct ttgcgcatat
     3901 cgcagtcgat caacaacacg cgtttattgg tctggctgat caccgccgcc aggttggcgc
     3961 agacaaaggt tttaccgatt gacgggctaa ccccggtcat catcaacaca ttgttctgcg
     4021 cctgcatcat cgcgaagtgc agactggtac gcagactacg gatggcttca atcgccagat
     4081 cggttggatt ccccactgcc agtagctggc tctgtttata gcgtttaacc cctttgatgg
     4141 ttttgacgct atcgcgcgct ttctgccact ccgacagcgg gatgctggca tagacgctga
     4201 ttccgtgttc ttccagcact tgcgggcttt cgatgccgcg attaaacaac gagcgcagca
     4261 gcacacccac gatagagagc atcaggccaa ggataatcgc gccgaggata atcagccctt
     4321 tcttcggttt tagcacgcca ggctgggtga ttgccgggtc aataatgcgc acatcgccga
     4381 cggtgctggc ttcggtgatt ttcagctcct gctctttatt cagcagttgc atatagacct
     4441 gctgaccaga ctcgacatcg cgggtcagac ggacaatttc ctgctggttt ttcggcatcg
     4501 ccgttacgcg accgttaagt ttggctttct cttcttccag cgcctgacgt ttctccagca
     4561 gcgtgcggta cgccgggtga actttggtgt acagcttgga gatttccgcc tctttaaagg
     4621 tcagttcgtt caactgcgcg tcgatgttca ccatcgaatc gagcaccgct ttcgcttcaa
     4681 gcggcagatc aacagaatct ttatcctgac ggaaggcatt cagtttgttt tcggcaacat
     4741 cgaggcggct acgtacttcc ggtaactgtt gcgcgaggaa agcgaggctt ttcgacgctt
     4801 ccgccgattt gcgctcaata ttttgttcct gatagttacg agcgatgctg ttaagaatgt
     4861 cgcggatctg ttcgcgatct tcaccggtat aagtcaggct cagtacgcct gcgtctttgc
     4921 cgttctccgt taccgtcagg ctgttttgca gttggttgat catccccaac gtggagtatt
     4981 tggtgacggt aaactcactg cccgggcggg cgtgaatggc ttcaaccatc agcgtgacgc
     5041 cttctttttt cagcatctgg cccgcttgcc cacgggcgct aaagccgcca tcactgctca
     5101 gggtgtagtt tttgtcatcc agcacattaa gcgtaaacac ctgatccgcc atctctttcg
     5161 ggcggttaaa ggtagtcact ttcaccgtct cgttctgacg ccccatcagg cgatcccagc
     5221 ccgccccgaa aatcgggaac gtgtttttgc tcaccgcaat atcgaggtcg agatcgtcca
     5281 ccgttttacc aagcaccaga cgcgaacgaa tcaactggat ctcggcgtcc gatgcaggcg
     5341 gtttgttggc taatgccgag ccgatatcct gcaccagcga attgccgctg ttttgctcga
     5401 tttgtaccag tgcgtcggca ctataaatcg gcgtggcgaa gaaagtgtaa accacggcac
     5461 agagggcaaa tacgacggtg atgccaatca cccaccagcg cgcttcaatg acggtgccga
     5521 ccaggcgacc aatatcgatt tcatcactgc ccgttaccgg agcggcatgt tgttttactt
     5581 tttctgtcat tcttatacct gctctgcgtt caatgcctgc gcccactggc gggcagaccg
     5641 ttcaagtaat gtgtacaccg ctgcaaacgt ttcccggctt ttgtgatacg gatcggggat
     5701 ttcacattcg ttatcccagt gaccaaacag catcactttg ccgcgcatct ccggtgccat
     5761 ctcgcataag cgttcgatat ggcgcttatc catggtcaaa atcaggtcat agttgcgaca
     5821 caggcggcgg ctgatttgtc gtgcacagtg gccttccaga gacagttgat gttctgcggc
     5881 gacgctgata gcggtaggat cagcgccctt accgaccagc gcgccgagtc cagcggactc
     5941 cactttcagt tccgggtgat aacgttgcag taagcgttcc gccatcgggg aacggcaaat
     6001 attgccgaca cagacaacta agatgttgtt aaacatgacg attaccagtt atgaatgtcg
     6061 ctggctgtat ccgtcatgta acggacaccg ctaatagttg gcagcaactg attgatcaga
     6121 cggttccagc gggaaaccgg ggcggtggtg acatacacca cgtcataagg ttgcaggcgg
     6181 aattctgtcg ccatcaccag cgacgtcgca tcggacatat ccagctggta gatattggca
     6241 atcttgccgt tacgcccgcc ctcgcctttc agcggacgaa tgacaaagat gccgctggcg
     6301 ttggaggtgg tcatgtcgat accttcggca ttggccagag cttcagtcag ggtcatgccg
     6361 ctaaagtcca ttttcagggt gctctgtttc ttcacttcac ccatcacaaa tactttcaga
     6421 tcatcattac gcggcacgta gagaatatcg ccgggataaa gcagtcggtt ctgattgagg
     6481 tcgccgtttt gcatcagcgc ctgtaaagaa atgcgttctt cacgaccatt gtgtgttagc
     6541 accacgttgc gccagtcagc ggtgtcggtc aggccacctg cggcgttgat ggcgtcgaga
     6601 atggtcagtg gtacgttggt gatcgcctgt tgaccggatt tattcacctg acctgagata
     6661 taggcctttt gtgagcggaa ggcggcgata ttaacgtcca cctgcgggtc agcgatgtac
     6721 gtcgctaagc gcccggtaat atcactgcgg atttcagcga gcgttttccc gactacgtga
     6781 accttgccga tatacgggta aaacatagtg ccgtcaggct gtacccagtt gccggtgtcg
     6841 ctggagctgc ggtactgacc agctggcgtg gtgagttccg ggtgatccca gacggtgaca
     6901 ttaagaacgt cccccggccc gacgcgatac tggtaattcg cgatctcgct ttccagcgtc
     6961 atattggggc gcgctacatt cgggcgtggg cgtaattggt caatcaggcg cggggtcagc
     7021 ggataaacat tcaccatttt gtcgagatcg aaatcagcgt cctgctgttt gatgacgtct
     7081 ttgcccatcg tcgacatatt gctgcccgga agtactgtgc aaccgcttat caaggttact
     7141 gacaccaata atggcatcaa tttcattttg gatttcatca ttgtttattt atcactttgg
     7201 cagagtaatt atcctgtgca ctattaatag caatgtcgcc atgcacattt accttgcagt
     7261 taattgaata aaaatttaac tggcatcagt cctaaaaaaa ttgatttcat ccgcaggcta
     7321 ttgacagaat aattcagact ggtctttcag gcatccagac acgctaccgc ccctggcttt
     7381 ttagctacca atacactgat tgagcttaat ttttcaaacc ctctctgcat acaatcgttg
     7441 atgagaaagg gttattgcgg aaattaactt ccgaatataa ggtgacatta tggtaattga
     7501 atattggctc gccaataatg caggaggaag tgttacagct aacggaatag caggcaaggt
     7561 aacaattcgg caattgg
//
LOCUS       DAZSIO010000170         7538 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZSIO010000170 DAZSIO010000000
VERSION     DAZSIO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.172"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..1160
                     /gene="rbsA"
                     /locus_tag="ACHX5T_003439"
                     /pseudo
     CDS             1..1160
                     /gene="rbsA"
                     /locus_tag="ACHX5T_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1165..2130
                     /gene="rbsC"
                     /locus_tag="ACHX5T_003440"
     CDS             1165..2130
                     /gene="rbsC"
                     /locus_tag="ACHX5T_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR8468487.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2155..3045
                     /gene="rbsB"
                     /locus_tag="ACHX5T_003441"
                     /pseudo
     CDS             2155..3045
                     /gene="rbsB"
                     /locus_tag="ACHX5T_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3171..4100
                     /gene="rbsK"
                     /locus_tag="ACHX5T_003442"
     CDS             3171..4100
                     /gene="rbsK"
                     /locus_tag="ACHX5T_003442"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR8468488.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4104..5096
                     /gene="rbsR"
                     /locus_tag="ACHX5T_003443"
     CDS             4104..5096
                     /gene="rbsR"
                     /locus_tag="ACHX5T_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR8468489.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5062..6489)
                     /gene="mdtD"
                     /locus_tag="ACHX5T_003444"
     CDS             complement(5062..6489)
                     /gene="mdtD"
                     /locus_tag="ACHX5T_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR8468490.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6512..7204)
                     /locus_tag="ACHX5T_003445"
     CDS             complement(6512..7204)
                     /locus_tag="ACHX5T_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR8468491.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1918 a         1827 c         1986 g         1807 t
ORIGIN      
        1 aaaaccatgt atgtcgaagc ggataaattg ctggctaaac ttaacctgcg ctttaaaagc
       61 gacaagttag tgggcgatct ttccatcggt gaccagcaaa tggttgaaat cgccaaagtg
      121 ctgagctttg agtcgaaagt catcattatg gatgaaccga ccgatgcgct gaccgatacc
      181 gaaaccgaat ccctgttccg cgtcatccgc gagctgaaat cgcaaggcca ctgtattgtc
      241 tatatctccc accgcatgaa agaaatcttc gagatttgcg atgacgttac cgtttttcgt
      301 gatgggcaat ttattgctga gcgcgaagtg gcatcactga ccgaagattc gctgattgag
      361 atgatggtgg gccgcaagct ggaagatcaa tatccacacc tggacaaagc gccaggagat
      421 atccgcctga aagtcgataa tctctgcgga cctggcgtta acgatgtctc ttttacttta
      481 cgcaaaggcg aaattcttgg cgtctctggt ttgatgggcg caggtcgtac cgaactgatg
      541 aaagtgctct acggcgcact gccgcgcacc agcggttacg tcaccctgga tgggcatgaa
      601 gtcgttaccc gttcaccgca ggatggcctg gcaaacggca ttgtgtatat ctccgaagac
      661 cgtaaacgtg acggtttagt gctgggcatg tcagtaaaag agaacatgtc gctgacagcg
      721 ctgcgctact tcagccgcgc tggcggcagt ttgaagcatg ccgatgaaca gcaggctgtg
      781 agtgatttca ttcgtctgtt taatgtgaaa acgccgtcga tggaacaggc aattggtctg
      841 ctttccggtg gcaatcagca aaaagtggcg attgcccgcg gtctgatgac acccccaaag
      901 tattgatcct tgatgagcct acccgtggcg tagatgtcgg cgcgaaaaaa gagatctatc
      961 aactgattaa ccagttcaaa gccgatggct tgagtatcat tctggtgtca tcggagatgc
     1021 cagaagtatt aggcatgagc gatcgcatca tcgtcatgca tgaagggcat ctcagcgggg
     1081 aatttactcg tgagcaggcc acccaggaag tgttaatggc tgtcgctgtg ggcaagctta
     1141 atcgcgtgaa tcaggagtaa aaaaatgata acccagactg tctctggtcg ccgttatttc
     1201 acgaaagcgt ggctgatgga gcagaaatcg cttatcgctc tgctggtgct gatcgcgatt
     1261 gtctcgacgt taagcccgaa ctttttcacc atcaataact tattcaatat tctccagcaa
     1321 acttcggtga acgccattat ggcggtcggg atgacgctgg tgatcctgac gtcgggcata
     1381 gacttgtcgg taggttctct gttggcgcta accggcgcag ttgctgcatc tatcgtcggc
     1441 attgaagtca atgcgctggt ggctgtcgct gctgctctca cgttaggtgc cgcaattggt
     1501 gcggtaaccg gggtgattgt agcgaaaggt cgcgtccagg cgtttatcgc tacgctggtt
     1561 atgatgcttt tactgcgcgg cgtgaccatg gtttatacca acggtagccc agtgaatacc
     1621 ggctttactg agaacgccga tctgtttggc tggtttggta ttggtcgtcc gctgggcgta
     1681 ccgacgccag tctggatcat ggggattgtc ttcctcgcgg cctggtacat gctgcatcac
     1741 acgcgtctgg ggcgttacat ctacgcgctg ggcggcaacg aagcggcaac gcgtctttct
     1801 ggtatcaacg tcaataaaat caaaatcatc gtctattctc tttgtggtct gctggcatcg
     1861 ctggccggga tcattgaagt ggcgcgtctc tcctctgcac aacccacggc ggggactggc
     1921 tatgagctgg atgctattgc tgcggtggtt ctgggcggta cgagtctggc gggcggaaaa
     1981 ggtcgcattg ttgggacgtt gatcggcgca ttaattcttg gcttccttaa taatggattg
     2041 aatttgttag gtgtttcctc ctattaccag atgatcgtca aagcggtggt gattttgctg
     2101 gcggtgctgg tagacaacaa aaagcagtaa taacgactac aggacatctt gaatatgaac
     2161 atgaaaaaac tggctaccct ggtttccgct gttgcgctaa gcgccaccgt cagtgcgaat
     2221 gcgatggcaa aagacaccat cgcgctggtg gtctccacgc ttaacaaccc gttctttgta
     2281 tcgctgaaag atggcgcgca gaaagaggcg gataaacttg gctataacct ggtggtgctg
     2341 gactcccaga acaacccggc gaaagagctg gcgaacgtgc aggacttaac cgttcgcggc
     2401 accaaaattc tgctgattaa cccgaccgac tccgacgcag tgggtaatgc tgtgaagatg
     2461 gctaactagg cgaacattcc ggttatcact cttgaccgcc aggcaacgaa aggtgaagtg
     2521 gtgagccaca ttgcatctga taacgtactg ggcggcaaaa tcgccggtga ttacatcgcg
     2581 aagaaagcgg gtgaaggtgc caaagttatc gagctgcaag gcattgccgg tacatccgca
     2641 gcccgtgaac gtggcgaagg cttccagctg gccgttgctg ctcacaagtt taatgttctt
     2701 gccagccagc cagcagattt tgatcgcacc aaaggtttga acgtaatgca gaacctgttg
     2761 accgctcatc cggatgttca ggctgtattc gcgcagaatg atgaaatggc gctgggcgcg
     2821 ctgcgcgcac tgcaaactgc cggtaaatcg gatgtgatgg tcgtcggatt tgacggtaca
     2881 ccggatggcg aaaaagcggt gaatgatggc aaactagcag cgactatcgc tcagctaccc
     2941 gatcagattg gcgcgaaagg cgttgaaacc gcagataaag tgctgaaagg cgagaaagtt
     3001 caggctaagt atccggttga tctgaaactg gttgttaagc agtagtttta atcaggttgt
     3061 atgacctgat ggtgacataa atacgtcatc gacagatgaa cgtgtaatat aaagaaaagc
     3121 agggcacgcg ccaccctaac acggtggcgc attttatgga catcccgaat atgcaaaacg
     3181 caggcagcct cgttgttctt ggcagcatta atgctgacca cattcttaat cttcaatctt
     3241 ttcctactcc aggcgaaacc gtaaccggta accactatca ggttgcattt ggcggcaaag
     3301 gcgcgaatca ggctgtggct gctgggcgta gcggtgcgaa tatcgcgttt attgcctgta
     3361 ctggcgatga cagcattggc gagagcgttc gccagcagct cgccactgat aacatcgata
     3421 ttactccggt cagcgtgatc aaaggcgaat caacaggtgt ggcgctgatt tttgttaatg
     3481 gcgaaggtga gaatgtcatc ggtattcatg ccggcgctaa tgctgctctt tccccatcgc
     3541 tggtggaagc gcaacgtgag cgtattgcca acgcgtcagc gttattaatg cagctggaat
     3601 caccactcga aagtgtgatg gcagcggcga aaatcgccca tcaaaataag actatcgttg
     3661 cgcttaaccc ggctccggct cgcgaacttc ctgacgaact gctggcgctg gtggacatta
     3721 ttacgccaaa cgaaacggaa gcagaaaagc tcaccggtat tcgtgttgaa aatgatgaag
     3781 atgcagcgaa ggcggcgcag gtactgcatg aaaagggtat ccgtactgta ctgattactt
     3841 taggaagtcg tggtgtatgg gctagcgtga atggtgaagg tcagcgcgtt cctggattcc
     3901 gggtgcaggc tgtcgatacc attgctgccg gagatacctt taacggcgcg ttaatcacgg
     3961 cgttgctgga agaaaaacca ttgccagagg cgattcggtt tgcccatgct gccgctgcga
     4021 ttgccgtaac acgtaaaggc gcacaacctt ccgtaccgtg gcgtgaagag atcgacgcat
     4081 ttttagacag gcagaggtga cgcttggcta caatgaaaga tgttgcccgt ttggcgggcg
     4141 tttctacctc aacagtttct cacgttatca ataaagatcg cttcgtcagt gaagcgatta
     4201 ccgccaaagt tgaagcggcg attaaagaac tcaattacgc gccatcagct ctggcgcgta
     4261 gcctcaaact caatcaaaca cataccattg gcatgttgat cactgccagt accaatcctt
     4321 tctattcaga actggtgcgt ggcgttgaac gcagctgctt cgaacgcggt tatagtctcg
     4381 tcctttgcaa taccgaaggc gatgaacagc ggatgaatcg caatctggaa acgctgatgc
     4441 aaaaacgcgt tgatggctta ctgttactgt gtaccgaaac gcatcagcct tcgcgtgaaa
     4501 tcatgcaacg ttatccgaca gtgcctaccg tgatgatgga ctgggctccg ttcgatggcg
     4561 acagcgatct tattcaggat aactcgttgc tgggcggaga cttagcaacg caatatctga
     4621 tcgataaagg tcatacccgt atcgcctgta ttaccggtcc gctggataaa actccggcgc
     4681 gcctgcggtt ggaaggttat cgggcggcga tgaaacgtgc gggtctcaac attcctgatg
     4741 gctatgaagt cactggtgat tttgaattta acggcgggtt tgacgccatg cgccaactgt
     4801 tatcacatcc gctgcgtcct caggccgtct ttaccggaaa tgacgctatg gctgttggcg
     4861 tttaccaggc gttatatcag gcagagttac aggttccgca ggatatcgcg gtgattggct
     4921 atgacgatat cgaactggca agctttatga cgccaccatt aaccactatc caccaaccga
     4981 aagatgaact gggggagctg gcgattgatg tactcatcca tcggataacc cagccgaccc
     5041 ttcagcaaca acgattacaa cttactccga ttctgatgga acgcggttcg gcatagattt
     5101 acgctgtctt ttgatcaaat tattaccatc ggttgttttc agaagcatga acattgctgc
     5161 tgaagcaaca gtaataatgc ccatcgtgat aaacgtatag tggaattgtt cgacagtcgt
     5221 tgtgccttcc atcccttcat aaatgcgaag gacggccgca cttacagcaa cgcctaaact
     5281 aatcgacagt tgctgcgtga ccgccagaac actgttaccg ctgctggcgt tgtcatcggt
     5341 cagatcggca agtgtgatgg tattcatcgc ggtaaactgc gtcgacatag ccatccctaa
     5401 tataaacaac ggcaagatca gcatccatat cgccattgcc ggtgattgca aagagaactg
     5461 agcgatcatt agcccaataa tcaccgtgat ccccactaat gtatggcgat agcccagacg
     5521 acgtaagact tgggtaacca tcgattttgc aataatggaa cctaacgctg tcggtgccat
     5581 catacagccg gcaataaacg cctgataacc aaatcctacc tgtaacatca atggcataag
     5641 gaacggtaca cagccggtcc ccagacgggt tgcaatattg cctacgatac cgatcgagaa
     5701 agtgcgggtt ttaaataaat ctaatgaaat taatgggttt ggcgtacgtc gcgcatggag
     5761 aatatagaga agcagtaacc cgatgctggt gacaattacc gtcaaggcaa tccagctggc
     5821 gacaatcttt tccccgaata gctctattcc gcttgaaaag agaacaaggc tgaggccaaa
     5881 cagcaaaaag ccagtgatat cgaatctgcg tcgtgcggtg gtgaaattgg gcatatgttt
     5941 gcgcgcgtaa agaaggcccg caatacctat ggggatatta attaaaaata tccagtgcca
     6001 ggttgcccag gtgaccagca cgccgccaag aacggggcct aaaattggcc ccaccagacc
     6061 cggcatggcg acaaaattca ataccggaag aagttcatta cgaggataag cacgcagtaa
     6121 ggccggccga gcaacaggca tcatcattgc gtcgcctatc ccttgaataa cccggaagac
     6181 aaccagctgt ggtagcgaat tagaaagtgc gcaggccaga gaacccaacg tgaacagact
     6241 cacggcaagg gtaaaaatgc gacgcgtacc gaagcgatcg gctagccatc cgcttaccgg
     6301 aataagcatc gccaccgtca gcgtataact gatgatggct gattgcatcg cgagaggaga
     6361 acgattaagg ctatgagcga ttgcgggtaa ggcggtattc agaatagtgg catcaagtgc
     6421 ctgcatgaag aaggccatcg ccgcgatcca cggcaaaccc gacatactac gcttcttttt
     6481 atcgctcatt caatgtcctg ttatcgggtt atcacttatc aggtgagcgt agcagcgcct
     6541 gacaagcttt aaatgccgcg tcgccatcgc tttggataat cgcatcgaca atcgcctggt
     6601 gcagatccag ctttatcact gtgtcgctgg taattgacgt gaagtaagtg tgataaaccg
     6661 aatggaatag cgaggcgaat gaggtcaaaa acggattggc gctcatttca tagatatgct
     6721 catgccaggc catatcgact tcgatccagc gttcacggcg aaagttctct tttaatgccg
     6781 ccatttcggc cattaacgta ttgagatgcg ccttctgttc cgcggtgcca accgttgctg
     6841 ccagtaggca ggcttgcggc tccagacaga tacgcataac cagaaagtga tcgatgacct
     6901 gatgaaagtt ctcttctgtt atccaccaag taagcaattc ctgatcaaga aaattccagt
     6961 tcgattgtgg catgacccga gtaccaattc gcggtcgcgg taaaaccatc ccttttgccg
     7021 ttaacgtttt gaccgcttcg cgtaccgctg tacgactcac tccaaattgc tcgcccagct
     7081 caatctcacc gggcaaaatg gtgccgggtt catattcacc ttttaagatc cgttgcgcca
     7141 gcttctcagc cagaacatac gaaaggtttt tctgtgcagc taactgttgt gcgcttaaag
     7201 gcattactta tcttcctttt tctttttatt cctccttagt atgccaccag gaagtgtgat
     7261 tacggttgca aaaacggcaa attgcttgtt ttatggcaca ttaacggggt gttttgctga
     7321 aaaaatgcgc ggtcagaaaa ttattttaaa tttcctcttg tcaggccggg ataactccct
     7381 ataatacgcc accactgaca cggaacaacg gcaaatacgc cgccgggtca gcggggttct
     7441 cctgagaatt ccgacagaaa aagcaaaaat aaatgattga ctctgtagcg ggaaagcgta
     7501 ttatgcacac ccccccgcgc cgctgagaaa aagcgaag
//
LOCUS       DAZSIO010000171         7406 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZSIO010000171 DAZSIO010000000
VERSION     DAZSIO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.173"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(1..441)
                     /locus_tag="ACHX5T_003446"
                     /pseudo
     CDS             complement(1..441)
                     /locus_tag="ACHX5T_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(416..1216)
                     /gene="otsB"
                     /locus_tag="ACHX5T_003447"
     CDS             complement(416..1216)
                     /gene="otsB"
                     /locus_tag="ACHX5T_003447"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR8468492.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1383..2369)
                     /gene="araH"
                     /locus_tag="ACHX5T_003448"
     CDS             complement(1383..2369)
                     /gene="araH"
                     /locus_tag="ACHX5T_003448"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR8468493.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2384..3898)
                     /gene="araG"
                     /locus_tag="ACHX5T_003449"
     CDS             complement(2384..3898)
                     /gene="araG"
                     /locus_tag="ACHX5T_003449"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR8468494.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3968..4957)
                     /gene="araF"
                     /locus_tag="ACHX5T_003450"
                     /pseudo
     CDS             complement(3968..4957)
                     /gene="araF"
                     /locus_tag="ACHX5T_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5754..6257
                     /locus_tag="ACHX5T_003451"
     CDS             5754..6257
                     /locus_tag="ACHX5T_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR8468495.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6337..6588)
                     /gene="yecJ"
                     /locus_tag="ACHX5T_003452"
     CDS             complement(6337..6588)
                     /gene="yecJ"
                     /locus_tag="ACHX5T_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR8468496.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="ACHX5T_003453"
     CDS             complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="ACHX5T_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR8468497.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7052..7375
                     /locus_tag="ACHX5T_003454"
     CDS             7052..7375
                     /locus_tag="ACHX5T_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR8468498.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT         1911 a         1917 c         1678 g         1900 t
ORIGIN      
        1 caatgcgatt attcacccca cgtttgcgta attcatgcgc aaagggcaac aggtgataat
       61 cgtggatcca gataatgtca tcgtcttgca acagcggcag taatttatct gccagcaacg
      121 catttacgcg tagatagccg tcccaggcag gacgctgaaa ttgcaccaga tcgagccgat
      181 aatgaaaagc gggccagaga acggcattgg agaattggtt gtagtattcg tcaaggtcct
      241 gttcgctgag gttaaaagag gcccacgtaa tgttaccttt tttagcggct gatcctcatt
      301 ccctgtttca ccactccagc caaaccacag tccgcctgcg gctttcagtg cccccagtat
      361 gccaacggca aggccaccgg cactggcggc gtgctcgtct ggtggtgcaa tccggttaga
      421 tactacgact aaacgactca tagtcatcac tcctgttatt ttctcttttt tgttgtaatg
      481 cggcggttat catttcaagc cagctccaga catccggcac acccgccagt cgccatgatg
      541 cctgagttgc acctgtgcca atttttactg acattccgcc cagtcggtta acgactgcga
      601 agccagattc atcggttaaa tcatcgccca gaaatacggg cgttcgcccg ataaaaggag
      661 cttcctgcat aaaggctgca atcgcttcac ctttactggt acctctcggt ttgatctcga
      721 caacacactt tccctgctgt aacgccattt gtggccagat ctgagtaata cgttgcgcta
      781 atgtcattaa tgcgtcttca tgctgcggag cctgacgata atgcagcgca aaagccatcc
      841 ctttcgcctc cagctccgcg ccgggatact gagcgatgac cgtatgcagt tgcacgctaa
      901 tatcacgcgc aatcgcatcc ggcagatgaa cgatatgtgt tttaccattg atgtcacggc
      961 gctccgcccc atgcacgccc gctaacggga agcgataagg ttttgccagt gcgtcaagct
     1021 ccaccattga gcgccctgat atcaatgcca atgcaccatc acttgcggtt gccagtagct
     1081 gtagtccttg cagaatattg tcaggcacga cgacctgatc gggatgcggt ttgatttccg
     1141 ccagcgttcc atcaagatca aaaaaccagg catatttcgc ggatagttca ggggtttcgg
     1201 ttaacggttc tgtcacccgg ctctcctcct tctcttttct tattgtgttg cgcagaccgc
     1261 gctttacaga catgtaagta tagacagtgt gacggaggtc gccatttgca acgaagaaca
     1321 gccaaccgtt gtggcggctg gctgtggtgg gaaaaaacgt taagttgttg tggaaaaaag
     1381 catcagacag tgcgcttcgc tttttgcttg taacggtcga agatcaccgc tgccagcagg
     1441 attaagccgc gaaccacgta ctgcgcgaaa ggagaaatat taagcaggtt catggcgttt
     1501 tccacggtgc ctaaaattaa gatacccgcc accacatatg agatttttcc gatgccacct
     1561 ttcagagaaa cgccacctaa aacgcaggcg gagataacaa taagctcata accaatcgac
     1621 gtcattggct gcccactagt catacgtgaa gccagaataa ttccggctat cgcagatacc
     1681 aggccagaga gaacaaagat aataattttg gtgcgaacaa ccggtacacc cgccagacgc
     1741 gcagcctctt cgttcccgcc aatcgccagg gtattacgac caaaggtggt tttattcagc
     1801 agcaaaccaa agataatcag acacgcgacg gtgagccaga ttggcgcagg cagaccgaac
     1861 cagttagcgt aaccaagggc aaagaagctt tcatcttcga taccgaccgc tttaccgtct
     1921 gaaatgatgt acgccagacc tcgaacaatc tgcatcgttg ccagtgtcgt gatcagagca
     1981 tttattttca gtttggcgat aacaaagcca ttgaccaggc cacagagaac gcccagcaac
     2041 aaacccgctg ccacgccaat ccacaggctt tcagtcaggt tgataaccac cgccgtggtg
     2101 acacccgcac aggcaattac ggaggcgaca gaaaggtcaa agtcaccgga agcaaggcag
     2161 aacaacatgc cacacgccac catccccgac atggaaattg ccaggcccaa ccctttcata
     2221 ttaatgaagg tggcaaaatt tgggacaaaa atggcacagg cgataaagag caccgcaaac
     2281 accaccagca tgccgtactg atcccagata cgcccgaagc tgaatgacga cttaggtgcg
     2341 ccagaccccg atgtagaaac agaagacatc atactctcct tactcaggca actgcctggc
     2401 tgactttagg catcgcaagg ctcagtgcct gacgctcatc tgcctgctcg tgtaacaatt
     2461 caccggcgat ttcgccttcc cgcatcacca caatccggtc ggcaacgccg aggacttcag
     2521 gtaagtcgct ggaggcaaac agcaccgcca cgccctgcgc cgccagcgca taaattacgt
     2581 tatatatttc gtgcttagcg ccaacatcaa tgccgcgcgt aggttcatcc agcaaaatga
     2641 ccttcatctc ttccgataac cagcggccca gaatggcttt ttgctgattt ccgcctgaga
     2701 gattcatgat cagttgctcc gcgcccggcg ttttgatgtt gagcgaacga atgtgctgat
     2761 cggcattgtt ttcttcccaa ctgttgttga ttacacaacc gccgagcaca tgtttgcgtc
     2821 tggcactgat gttgatattg tcgcgaacgg agtgcacggg aataatgcct tccgctttgc
     2881 gagcttccgg gcagagcatc atgcctgcgg caatggcgtg gctcggttta cgaatatcga
     2941 tcggctgttg gtcgatataa acctgaccgg cggtgatttg cgtcccgcca aacaagcctt
     3001 tcattaattc gctacgcccc gcccctacca gaccaaacag cccaacaatt tcaccactgc
     3061 gaaccgccag acttattggc gtacgcacgc ctagtgcttt cacagcatca agacgcaggc
     3121 gctcctcgcc ataactacga ggttgccagc cgtagatatc gccaatgtcg cgcccgacca
     3181 tcgcctgcac cagcgcgtcg tggtcaacct gctgcatatc ggtaaaggtt ttgacataac
     3241 gtccatcttt aaagacagtg atggcatcgc tgagggcaaa tatttcttcc atacggtgag
     3301 aaacgtataa gatcacccgc ccctcttttc gcagttcacg aataacgcgg aaaagattgc
     3361 cgatttcgcg ggcagaaagg gagctggttg gctcatcaaa ggcgataatt ttagcgttac
     3421 gcgccagcgc tttggcgatt tcaaccatct gccactgacc aatggcgaga tatttcagcg
     3481 gcgtgtccgg gtcaatatcc ataccaagat gtttaagttg taaacccgcc tcataattca
     3541 gcaatgagcg attcacaatg ccgcctttat gcggcagctg gccgagatag atgttttccg
     3601 cgacggtcat ttccggcacg agatgcagtt cctggtaaat aatcgccaca cccgcgttaa
     3661 gtgctgcggt cgtgtcggaa aaggacattt cctgcccatt aatcactaca gaacccgtgg
     3721 ttggcgcata gttgccgctg aggattttta agagagttga ttttcctgcg ccattttcac
     3781 ccatcaacgc atgaacctga ccggcatagc agtcaaaact aatatccgtc agcgccttaa
     3841 cgccgggaaa cgttttaccg atgccgcgaa atgagagata cggggtagac tgttgcataa
     3901 cgtctccgtg aatcactggt cgttcacacc ccctctgcat catgcagagg ggaatttttc
     3961 cggcaaatta cttaccgcct aaaccttttt tctccagttc ttctttaaag ttgtcacgcg
     4021 tgatcagtac cacgtctgta acttcggtaa attttggcgt ttcaacgtct tttgctaccc
     4081 agttgtaaag catttcgctg gatttatagc catgtacgtc cgggcttggc agcagggaac
     4141 cgtagaagcc ggttgcctgt gctttagaca gttcgcttac tgcatccaca ccgttaatac
     4201 caataccgat gatatcggct gctttaaagc cctgaccttc ggttgcacgt acgccgccaa
     4261 gtacggtgct gtcgttcata ccgacgatca gccaatgttt aacttccgga tgttgaacca
     4321 gcattgagtt ggcagcgtca aatgcccccg ggatgtcgtt agatttggta ggtacctgat
     4381 aaatttgttt ttccgggaat cctgcagctt tcagtgcgtc catagaaccc gttgtacggc
     4441 ggcgggcggt atccagttcg ttggcggtaa tcgccatcac tgcgctttct ttgacatccc
     4501 agccacgttt ctgcatctct ttatacagtt cctagccctg acgttcgcca attttagtcg
     4561 ccgccatcat caccagagga acggtatcca ttggcttacc tttggcgtta acaaactggt
     4621 catccactgc aatgactttc atatcgtagc cacgcgcttt cgcgacgatg gcagagccga
     4681 gtttcgggtc cggagtacaa ataacgaaac cttttgcgcc actggctgcc aggctgtcga
     4741 tcgcgttcaa tgttttttcg ccatccggca cggcaatctt aataacctca aaccctaaat
     4801 ccttcccggc tttatcggca aacttccatt cggtctggaa ccacggctct tccggttgct
     4861 tcaccagaaa accgagcttc aggttctccg ccatagcgga ttgtgacata acggctgcca
     4921 gaccaatggc tgccagggct ttagtaaatt tgtgcatggt tctctccagc tttagtgtcg
     4981 ttttgtgtag ggcaaaaacg aatgacattc gttaaattaa tcggaaaaca aagcattacc
     5041 ttttatctaa aagataagtg accgtgttga catagtttta gcgagaaatt aattctccat
     5101 aggagagcaa tatcacatcg cagaattaca gtgagaacgt gcataaattt agcgggaaaa
     5161 gacataagga aaagccaatt tgtcagacaa attgttgaat gcacagcaga ttaatccata
     5221 agattagcct ggaaatcctt gttgtcttcg gtacccatgc gggatgtctt ctttttaacc
     5281 agtcaatagg ccgcattacc tggcgttgag tttttgaaat ggtgtaataa ccacaactca
     5341 aagatgtgga aaatgcgcgt cattcatttc gccattaatt atcactgtgc tcattaatta
     5401 acagaacacg tataatgaga gccatctcgc aaaaatgaaa aaacgtttta taaaatcatc
     5461 acttcatcat gaattcaaat tcatcgatta atatcaacaa gatacaaaaa gcactatcat
     5521 taaaattcat tgcatttaca ttgatttcac caatgaaatg taaaaatata taaacttgat
     5581 gatttaagca ttttcttata accgttcaga cgttattctt atttcagatc atcgtcagaa
     5641 ttgactccac gatcacattt cggaccggca gaaaggaatt attctgcaaa cagtaattat
     5701 ggtgttttga tttatcttgc acctctccac ttctggatat aaggatatta ggtatggcaa
     5761 ccgctggaat gcttctcaaa ctcaactctc aaatgaaccg cgagttttac gcatccaatc
     5821 tctaccttca cctgagtaac tggtgttctg aacagagtct gaacggcacc gccactttcc
     5881 ttcgcgccca ggcacagagt aatgtgaccc aaatgatgcg catgtttaac tttatgaaga
     5941 gtgtcggcgc tacccccatc gttaaagcca ttgatgttcc aggtgaaaaa ctgaactctc
     6001 tggaagaact gttccaaaaa acgatggaag aatacgagca acgttcaagc actctggcgc
     6061 agttagccga tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc ctgcgcgatc
     6121 tggaaaaaga acagcagcat gatggcctgt tgctgcaaac cattcttgat gaagtgcgca
     6181 gtgcgaaact tgcgggtatg tgccctgtgc agaccgacca acatgttctg aatgtcgtgt
     6241 cacaccagct gcattgatca tacatcggcg ctaatacatt gcgccgatga agtttttgag
     6301 aaaccgctgc ctcatctgtt tgaagcagcg gtttttttaa tgggattcac cctgtggggt
     6361 aaacttgagt tcaatcagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc
     6421 ggcagcggga tgatgagtaa agtcgatatt cttcagctga ctggacattt tttcacgaac
     6481 ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc
     6541 taccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg
     6601 atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac
     6661 cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc
     6721 tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg
     6781 cagtttcatc gcggcactcc gaccatttgt tatacaggtg ttattgtctt tgcgcttata
     6841 atatgacaac catcacaaaa atcaatcttt atgtgataca aatcacataa ataccccttt
     6901 aatgttataa aaatgataat caaaaaacag cccccctatt tctgacacct acagatggca
     6961 agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcgctgta acgcgtaaaa
     7021 tagtgctttc tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt
     7081 aataacgctg gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc
     7141 aacaggtatt gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg
     7201 ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcgat
     7261 cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac
     7321 ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt
     7381 ttaatcagcg aggggatctt cgctga
//
LOCUS       DAZSIO010000172         7375 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZSIO010000172 DAZSIO010000000
VERSION     DAZSIO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.174"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..1541
                     /locus_tag="ACHX5T_003455"
     CDS             <1..1541
                     /locus_tag="ACHX5T_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR8468499.1"
                     /translation="PTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVD
                     TYKIGIDKAIEAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGV
                     YSNTDAQDGIPLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFT
                     TFSAEEWQPQNRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVV
                     DRAKTLDQPFMLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKR
                     ILEQLKKNGQYDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKG
                     KLQPGNYDKLISAMDFYPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWI
                     TSYSHWFDEENIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVEN
                     NQLGLYKLTDLQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKAL
                     SEAK"
     gene            1593..2750
                     /locus_tag="ACHX5T_003456"
     CDS             1593..2750
                     /locus_tag="ACHX5T_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR8468500.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2716..2838)
                     /locus_tag="ACHX5T_003457"
     CDS             complement(2716..2838)
                     /locus_tag="ACHX5T_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468501.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3041..4726
                     /locus_tag="ACHX5T_003458"
                     /pseudo
     CDS             3041..4726
                     /locus_tag="ACHX5T_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4764..7124
                     /locus_tag="ACHX5T_003459"
     CDS             4764..7124
                     /locus_tag="ACHX5T_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR8468502.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7181..>7375
                     /locus_tag="ACHX5T_003460"
     CDS             7181..>7375
                     /locus_tag="ACHX5T_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468503.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSL"
BASE COUNT         2163 a         1615 c         1568 g         2029 t
ORIGIN      
        1 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
       61 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      121 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      181 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      241 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      301 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      361 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      421 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      481 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      541 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      601 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      661 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      721 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      781 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      841 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
      901 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
      961 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1021 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1081 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1141 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1201 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1261 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1321 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1381 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1441 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1501 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1561 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1621 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1681 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1741 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1801 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaata
     1861 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     1921 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     1981 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2041 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2101 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2161 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2221 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2281 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2341 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2401 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2461 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2521 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2581 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2641 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2701 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2761 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2821 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     2881 tcagggcatt agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa
     2941 aatttctata tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt
     3001 gccatagata atcattggat ggcacaatca attttcgcta atgataacca ttcccattac
     3061 gttttgtatg ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa
     3121 taataaaact tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa
     3181 aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga
     3241 aaccgataag ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt
     3301 aatatccgca aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt
     3361 aacagattac taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta
     3421 cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact
     3481 gatcaacaaa acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac
     3541 ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga
     3601 atggaatatc caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt
     3661 atttacccat aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga
     3721 agcgacattt cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag
     3781 caatgctgag tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa
     3841 ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg
     3901 ctcgcttagc gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa
     3961 tctgggcgga ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg
     4021 gtttatttat aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga
     4081 gttccatcaa ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt
     4141 acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa
     4201 ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac
     4261 cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc
     4321 tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat
     4381 tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca
     4441 ggttggtctg ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct
     4501 ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg
     4561 gatatttctt gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg
     4621 tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt
     4681 ctggaacctg gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc
     4741 ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg
     4801 gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga
     4861 tgacggtcta tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa
     4921 gcatcgagcg tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg
     4981 cggtacgcat ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga
     5041 aaatctctat tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg
     5101 caactaataa cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg
     5161 ggatgtcaca ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca
     5221 gttttgtgcc ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac
     5281 gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt
     5341 taacatcgca tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta
     5401 cctatttctc tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg
     5461 ctgataactt tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg
     5521 attatgtttc gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata
     5581 ctgcattgag caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa
     5641 aatataccgg ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa
     5701 tgggtaataa atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac
     5761 tacgtaccac cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct
     5821 ggtacaccga actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat
     5881 taggacacat ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa
     5941 aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg
     6001 atgcatggac tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga
     6061 aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc
     6121 tgtatatggc ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg
     6181 actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata
     6241 tttttgctga tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata
     6301 ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata
     6361 aaaccctgac gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat
     6421 tgcagcaaaa aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca
     6481 gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg
     6541 gcaaaaccaa aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc
     6601 gccaggtaga tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt
     6661 cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta
     6721 atctggtctc ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct
     6781 taaacatgga tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc
     6841 aagaagcgcg taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat
     6901 attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc
     6961 gcttgtcctg gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc
     7021 tcaatgtact ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg
     7081 ccagtggccg tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggattttact
     7141 taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc tctgtttctt
     7201 actgacgtta gtggcaactc tgttgctccc cgggcggctg attgccgccg ccttaccgca
     7261 ggatgaaaag ttaattaccg ggcaactgga caatgacttg cgatatatga tttatccgca
     7321 tgctcatcca aaggatcagg taaatttatg gctgcaaatt cataccggtt cattg
//
LOCUS       DAZSIO010000173         7364 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZSIO010000173 DAZSIO010000000
VERSION     DAZSIO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.175"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            118..225
                     /gene="yniD"
                     /locus_tag="ACHX5T_003461"
     CDS             118..225
                     /gene="yniD"
                     /locus_tag="ACHX5T_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR8468504.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(278..1036)
                     /locus_tag="ACHX5T_003462"
     CDS             complement(278..1036)
                     /locus_tag="ACHX5T_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR8468505.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            1323..2252
                     /gene="pfkB"
                     /locus_tag="ACHX5T_003463"
     CDS             1323..2252
                     /gene="pfkB"
                     /locus_tag="ACHX5T_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR8468506.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            2353..2643
                     /gene="ghoS"
                     /locus_tag="ACHX5T_003464"
     CDS             2353..2643
                     /gene="ghoS"
                     /locus_tag="ACHX5T_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR8468507.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            2749..3609
                     /locus_tag="ACHX5T_003465"
     CDS             2749..3609
                     /locus_tag="ACHX5T_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR8468508.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(3650..4186)
                     /locus_tag="ACHX5T_003466"
     CDS             complement(3650..4186)
                     /locus_tag="ACHX5T_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR8468509.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            4333..5001
                     /gene="hxpB"
                     /locus_tag="ACHX5T_003467"
     CDS             4333..5001
                     /gene="hxpB"
                     /locus_tag="ACHX5T_003467"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR8468510.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            5164..5754
                     /locus_tag="ACHX5T_003468"
     CDS             5164..5754
                     /locus_tag="ACHX5T_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR8468511.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            5887..7277
                     /gene="tcyP"
                     /locus_tag="ACHX5T_003469"
                     /pseudo
     CDS             5887..7277
                     /gene="tcyP"
                     /locus_tag="ACHX5T_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(7281..>7364)
                     /locus_tag="ACHX5T_003470"
     CDS             complement(7281..>7364)
                     /locus_tag="ACHX5T_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468512.1"
                     /translation="YESVCIAAMEHLKSKATAPIQNGPLPA"
BASE COUNT         1841 a         1807 c         1829 g         1887 t
ORIGIN      
        1 gaaacagtga ccatgtgcac aacgccaccc gacggctgta ataacaaaaa atcccttgat
       61 gcctgtccct tttgttacac tccgttatca cgcacaagag atatgcagga cactggtatg
      121 ccgactaaac gctttgataa aaaacactgg aagatggtgg tggtgctact ggcaatctgt
      181 ggcgctatgt tgttgctacg ttgggcagca atgatttggg gctgagcagt gacaaacgaa
      241 gacagccaga ccgcataaca cggtccggca gataatttta catgctgtag cccagcgata
      301 aggttgtccg acggtcggta tgttccggtg cagactctgg aggttcagag ttccatgtga
      361 cgttgtaagc caccttcagt ccaaaatgtt cattaatagc aacatttaat gcgctttcgg
      421 agttcagcgt tgtgtcttcc gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt
      481 cagtcaactg ccatgcataa gccccggagg catagcccag cggctgggtt tcactggcat
      541 tgtcggtata tttgtcgtaa cgcacacctg gaccgaattc aaagcggaaa ctgtgtaccg
      601 ggccattcaa aaactgacga ccataacccg cggtcaacac atcgcgctct cgatagccgt
      661 tataacggtc tgtcagccag cttgcctgac caaataaata gtcataatca gttaaattaa
      721 agcggctacg cccgcccgcc gcatattttt ctgaagaacg ctcatcatta gaagaagtat
      781 tactggcgtt cccccacagc gaccaggcag tggtttgtcc ataccaggtc atggtggtgt
      841 cagccgtaag tgaggagctt ttcgtattgc ctgattgtgc aagatatcct gcgttcagat
      901 taccttcgaa aggtttttta gcgctggcag gatcgtccat gacagtaaaa acggaatcat
      961 cggcagctgc attcagtgac gcaaacatgc cccccgccaa cataacgatg gcaggaactg
     1021 tcttcaaaag cttcatttat caagagtccg tacaacaaaa aaagagacca tcgcggtccc
     1081 ggaaactttc ttaaggatca aagattagcg tccctggaaa ggtaacgaat tataaaaagg
     1141 cgcgaataac ttagcaatgt attcttattt cattttttga ataagcatgt ggcgaaaaca
     1201 gatttttatt tatatatatt tatctgcaaa attttaaata aagctctaat aaatcatatt
     1261 gttaatttct tcactttccg ctgattcggt gccagactga aatcagccta taggaggaaa
     1321 tgatggtacg tatctatacg ttgacacttg cgccctctct cgatagcgca acaattaccc
     1381 cgcaaattta tcccgaagga aaactgcgct gtaccgcacc ggtgttcgaa cccgggggcg
     1441 gcggcatcaa cgtcgcccgc gccattgccc atcttggagg cagtgccaca gcgatcttcc
     1501 cggcgggtgg cgcgaccggc gaacacctgg tttcactgtt ggcggatgaa aatgtccccg
     1561 tcgctactgt agaagccaaa gactggaccc ggcagaattt acacgtacat gtggaagcaa
     1621 gcggtgaaca gtatcgtttt gttatgccag gcgcggcatt aaatgaagat gagtttcgcc
     1681 agcttgaaga gcaagttctg gaaattgaat ccggggccat cctggtcata agcggaagcc
     1741 tgccgccagg tgtgaagctg gaaaaattaa cccaactgat ttccgctgcg caaaaccaag
     1801 ggatccgctg catcatcgac agttctggcg aagcgttaag tgcagcactg gcgattggta
     1861 acatcgagtt ggttaagcct aaccaaaaag aactcagtgc gctggtgaat cgcgaactca
     1921 cccagccaga cgatgtccgc aaagccgcgc aggaaatcgt taatagcggc aaggccaaac
     1981 gggttgtcgt ttcccttggt ccacaaggag cgctgggtgt cgatagtgaa aactgcattc
     2041 aggtggtgcc accaccggtg aaaagccaga gtaccgttgg cgctggtgac agcatggtcg
     2101 gcgcgatgac actgaaactg gcagaaaatg cctctcttga agagatggtc cgttttggcg
     2161 tagctgcggg gagtgcagcc acactcaatc agggaacacg tctgtgctcc catgacgata
     2221 cgcaaaaaat ttacgcttac ctttcccgct aacaaaaaca ttcccccagc attgggggaa
     2281 tcaccaccaa cctgtcggca acgcgtttct ccgactatgc tcaaaagtca tgtgataaca
     2341 aaggggtgaa ctatggccag tggcgatctt gtccattatg tcataaccgt catgttgcat
     2401 gaggatacat tgactgaaat taacgagttg aataattacc tgactcgcga cggttttttg
     2461 ctcaccatga cggatgatga gggaaatatc catgagctgg ggactaacac ttttggactt
     2521 atcagtaccc aaagtgaaga agaaattaga gaactggttt cggggcttac ccaaagtgca
     2581 accggcaaag atcctgaaat caccatcacg acctgggagg aatggaatag caacagaaaa
     2641 taaatggttt ttgggcaata atcagtctgt ggtgtgcgtt agctcgtgtt tttacaccgc
     2701 attcttgcgc taaccttatg atctggcaga caacatggga gagacatcat gtggcaggca
     2761 atcagtcgtc ttttgagcga gcagttaggt gaaggcgaaa tcgaactgcg taatgaactg
     2821 cctggcggag aagtccatgc cgcatggcat ttgcgctatg caggacatga ctttttcgtc
     2881 aaatgtgatg aaagggaact gcttcccggt tttaccgccg aagccgacca actggagtta
     2941 ctgtcgcgta gtaaaaccgt caccgtgcct aaggtttggg cagtaggcgc tgaccgtgac
     3001 tacagttttc tggtgatgga ttatctccca cctcgtccgc tggatgcgca tagcgcattt
     3061 attcttggtc agcaaattgc gcgtttacat caatggagtg accaaccaca atttggcctc
     3121 gattttgata actcgctctc cacaactcca cagcccaaca cctggcaacg tcgctggtca
     3181 acgttttttg ctgaacaacg gattggctgg cagttggaac tggcagcaga gaaagggatc
     3241 gctttcggca atatcgacgc catcgtcgag catattcagc agcgtctggc ctcgcatcaa
     3301 ccgcagcctt ctctgttgca cggcgattta tggtccggca actgtgcgct gggtccggat
     3361 ggcccgtaca ttttcgaccc tgcctgcgac tggggtgacc gagagtgcga cctagcgatg
     3421 ttaccgctgc atactgaaca gccgccacaa atctatgacg gctatcagtc agtatccccg
     3481 ctacctgccg atttccttga acgtcaaccg gtttaccaac tctacacgct gttaaatcgt
     3541 gcaagattat ttggcggtca gcatttggtt attgctcagc agtcattgga tagattatta
     3601 gcagcatgat atgggttgag gataatggcc gctccgtgcg gccttttgat taaataaacc
     3661 caagcagaga aaagaacaca taacctgcag caatacaaat aaccggcagg atatatagtg
     3721 agaaaaactg caggaaaata gtatggtgcg gaacaacaat acgggactca atttgttcac
     3781 gcgttagccc ctcttcccct ttggcttttt ccagaatgag ttgatcttca acaccttccc
     3841 gcaagaagcg cgcctggcga ctcatccggg caccggaatc ctgcaacgcc agtccgacaa
     3901 aaatcaggat gaaaatcacc cagaacataa cgttcacacc accattaaaa ttaggggtcg
     3961 gggagttata ccagaacaga ttcaaaaaag gcgtattcgc ctgcatcata tcgatcatga
     4021 catgagcaaa gtcgagcatg accgcattaa tgccttcctg gttttcctgc cgggtattca
     4081 taaatttcag caaagaaatc aatgtagaga tcaatgcagg tataaaaatc acccaaccca
     4141 aaatcctttt caaaacagca atgcgtccag cttgttgata cgtcatgagt tctccttgat
     4201 taagacgcgt cgtttcactt agtttacctg tagatatctg ttttcgccca ttctttaaag
     4261 gcgatatgat aggcgcttaa tcataagcac ggcttaatac cttacacata atgctctaaa
     4321 ggagaggttg taatgtcaac cccgcgtcag attcttgctg caatttttga tatggatgga
     4381 ttacttatcg actcagaacc tttatgggat cgagccgaac tggatgtgat ggcaagcctg
     4441 ggggtagata tctcccgtcg taacgagctg ccggacacct taggtttacg catcgatatg
     4501 gtggtcgatc tttggtacgc ccggcaaccg tggaatgggc caagccgtca ggaagtagta
     4561 gaacgggtta ttgcccgtgc catttcactg gttgaagaga cacgtccatt attaccaggc
     4621 gtgcgcgaag ctgttgcgtt atgcaaagaa caaggtttat tggtgggact ggcctccgcg
     4681 tcaccactac atatgctgga aaaagtgttg accatgtttg acttacgcga cagtttcgat
     4741 gccctcgcct cggctgaaaa actgccttac agcaagccgc atccgcaagt atatctcgac
     4801 tgcgcagcaa aactgggcgt tgacccactg acctgcgtgg cgctggaaga ctcggtaaat
     4861 ggcatgatcg cttctaaagc agcccgcatg cgttccatcg tcgttcctgc gccagaagcg
     4921 caaaatgatc cacgttttgt attagcaaac gtcaaactct catcgctgac agaactcacc
     4981 gcaaaagacc ttctcggtta atgaccaggg gcagtgatca cgctgcccct ggttctttat
     5041 ctgaatttgc attcaactga cggattaatc gtcaatttaa gagaaagagt tacaccgtca
     5101 ccacttccgt gcactgtata aaaatcctat actgtacgta tcgacagttt agtgagtttt
     5161 atcatgacgg cggaaggtca ccttctcttt tctattgctt gtgcggtatt tgccaaaaat
     5221 gccgagctga cgcccgtgct ggcacagggt gactggtggc atattgtccc ttccgcaatc
     5281 ctgacgtgtt tgttaccgga catcgatcac ccaaagtcgt ttcttgggca gcgattaaaa
     5341 tggatatcaa aaccgatcgc ccgcgctttt gggcatcgtg gttttaccca cagtctgctg
     5401 gcggtatttg cgctgctggc aaccttttac cttaaggttc cggaaggctg gttcattccg
     5461 gctgatgcgc tacaaggaat ggtgctgggt tatttgagcc acatacttgc cgatatgctg
     5521 acacccgccg gtgttcccct gctctggcca tgccgctggc gtttccgctt gcctatcctg
     5581 gttccccaaa agggcaacca actggaacgt tttatctgca tggcattatt tgtctggtcg
     5641 gtatggatgc cccattcatt acccgagaac agcgctgttc gttggtcatc gcaaatgatc
     5701 aataccttgc agatccagtt tcatcggctt attaagcatc aggttgaata ctaaaaaggc
     5761 aaaaatcacc tttctggaat aagcaattcc atttgaatat aagagccagc tcacagttct
     5821 gttaatcttg cgccaacact atgactgcta cgcagtgata gaaataataa gatcaggaga
     5881 acggggatga actttccatt aattgcgaac atcgtggtgt tcgttgtact gctgtttgcg
     5941 ctggctcaga cccgccataa acagtggagt ctggcgaaaa aagtgctggt gggtctggtg
     6001 atgggtgtgg tttttggcct tgccctgcat accatttatg gttctgacag ccaggtactt
     6061 aaagattctg tacagtggtt taacatcgtt ggtaacggct atgttcaact gctgcaaatg
     6121 atcgttatgc cgttagtctt cgcctctatt ctgagcgcgg ttgcccgtct gcataacgca
     6181 tctcagttag gcaaaatcag ttttctgacc atcggtacgc ttttgtttac cacgctgatt
     6241 gcggcgctgg tcggtgtgct ggtcaccaac ctgtttggtt tgacggctga aggtctggtt
     6301 cagggtggtg cagaaactgc acgtctgaac gccattgaaa gtaactatgt tggtaaagtc
     6361 tctgatcaga gcgttccgca gctggtcttg tcctttatcc cgaaaaaccc gtttgccgat
     6421 cttaccggag ccaatccgac gtcaattatc agcgtggtaa tttttgccgc attcctcggc
     6481 gtagctgcat tgaaactgct gaaggatgat gcgccgaaag gggaacgcgt cttaaccgct
     6541 atcgataccc tacaaagctg ggtgatgaaa ctggttcgcc ttgtcatgca gttgacccct
     6601 tacggcgttc tggctctaat gaccaaagtg gttgcaggtt ctaacttgca agacatcatc
     6661 aaactgggaa gtttcgttgt cgcgtcctac ctcggtctgc tgattatgtt tgctgtgcat
     6721 ggcattctgc tgggcattaa tggcgtgagt ccgctgaagt acttccgtaa ggtatggcct
     6781 gtgctgacgt ttgcctttac cagccgttcc agtgctgcgt ctatcccact gaatgtggaa
     6841 gcacaaacgc gtcgtctggg cgttcctgaa tccatcgcca gtttcgccgc ctctttcggt
     6901 gcaaccattg gtcagaacgg ctgcgccggt ttgtatccgg caatgctgcg gtgatggttg
     6961 cgcctacggt tggcattaac ccgctggacc cgatgtggat tgcgacgctg gtcggtattg
     7021 ttaccgttag ttccgcaggc gttgccggtg tcggtggtgg tgcaactttc gccgcactga
     7081 ttgtgctgcc tgcgatgggc ctgccagtaa ccctggtggc gctgttaatc tccgttgaac
     7141 cgcttatcga catgggccgt acggcgctaa acgttagtgg ctcgatgaca gctggcacgc
     7201 tgaccagcca gtggctgaag caaaccgata aagccattct ggatagcgaa gacgacgccg
     7261 aactggcaca ccgttaattc ttatgctggc aacggtccgt tttgtatagg ggccgttgct
     7321 ttacttttta aatgttccat cgcggcgatg catactgact cata
//
LOCUS       DAZSIO010000174         7363 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZSIO010000174 DAZSIO010000000
VERSION     DAZSIO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.176"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACHX5T_003471"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACHX5T_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFR8468513.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="ACHX5T_003472"
     CDS             complement(1433..1759)
                     /locus_tag="ACHX5T_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFR8468514.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="ACHX5T_003473"
     CDS             1894..2235
                     /locus_tag="ACHX5T_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFR8468515.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="ACHX5T_003474"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="ACHX5T_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="ACHX5T_003475"
     CDS             complement(2833..3543)
                     /locus_tag="ACHX5T_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFR8468516.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="ACHX5T_003476"
     CDS             3651..3956
                     /locus_tag="ACHX5T_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFR8468517.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..5568
                     /locus_tag="ACHX5T_003477"
                     /pseudo
     CDS             4155..5568
                     /locus_tag="ACHX5T_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(6298..7287)
                     /locus_tag="ACHX5T_003478"
     CDS             complement(6298..7287)
                     /locus_tag="ACHX5T_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468518.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
BASE COUNT         1945 a         1873 c         1762 g         1783 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagtgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgacaggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc
     4441 tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca
     4501 atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc
     4561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta
     4621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     4681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     4741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     4801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     4861 cgcatgagcg gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     4921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg
     4981 ctccctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     5041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     5101 accttacccg cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt
     5161 gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac
     5221 cgtatcatca aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc
     5281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct
     5341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat
     5401 accattacga tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc
     5461 ttcagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaactacaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc aggaccctcc ggtggcctcg
     5701 gtctggactt gaggcgggct atgggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagta caaacctaag cccccagaag
     5821 cccacccaca gccttagaca gagtgtcgaa tacattcaca ccgagtcgac cggagaggac
     5881 catatagagg cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag
     5941 gtcttcggaa gtcatggaca taaagccccc cctatcttct attgcactac cgcactagac
     6001 aactagtaca aaacaaaggc ccagaccatt ggtccagacc gtagttagtt aatcacagtg
     6061 acaagccacc acctgaagtc ctgctgatat tcagtccaga cttacctctg ctcatggtct
     6121 tacctccctc ggtctcctga ccttgggtat cgactacagc ttgctgagcg tcatctggag
     6181 gagctacagg aggagctaca ggaggcggga ccgaagcccc accttgagtc ccaccaccac
     6241 ctgtcaggcc cttggcgatg gacctgaagc ctttactaat gctcttaccc attggtctta
     6301 cgcttcggtc ttggtgaact tcaggcgttt gactacaggc aggacggcag tagctgagcc
     6361 agtgcccatc ttgatggtcg tagcagcttc agcgacaacg aacactttac cgaggatttc
     6421 caagtcgtgg gactgagagt cgccatcacg tgaaaggacg accgtaatat tctcaccgtc
     6481 gtgacggaag cagccgaagc tcagggccgt gaagcgttta gccagcatga atacaccctt
     6541 gtcttcaccg ttgtaatagg cgatgtctgt ccccagcata tcgacgatag cgtcttcacc
     6601 ggagaagcct tgcttagcag ccagacggga cagtgctttc agttggctct catgagcgat
     6661 aataccgtag tcagacacct cagaaccgaa agaagactct gtgttctcat acagagcgtc
     6721 catgatagcg tctgccttag cggtcaggct accagtcagg acaggaagct ctacggtctt
     6781 cagcagctta gaggattcca gaacgtcgac catgcgagcg ttgatggtac ggaccacatt
     6841 ctcacagatg ttcatagcgt cctgctgaag tgtgccctga gtgaatccgg tggatgactt
     6901 ggcgtcccag agcttggtct gctcagagat gagaatagcc tgctcgattg gttcgaccac
     6961 gatttcttgt ccagacggaa cggtcacgac ttcaggtcgc ttgccaccac ggacaggaac
     7021 gaccacagag cggtcagcca gagtgtgagc aatgattggg gtcggtaagc tgttacgggt
     7081 cggcaggact tctacaggga acgggatgtt ctcttgagcg tctttaagta caccgttcag
     7141 catagcctta accgtcttag ctaactggag ggcatagacc tgcgggtcag cgttagtcgg
     7201 cagaccttca ttgacctgaa tgttgtcggt atcggtgatg cgtactggct caatgccaaa
     7261 ctggaaagcg tctttaatcg tagtcataat aatcaatctc ctaaattagt ttaaatactg
     7321 tgagtcctca aaactgagga gtccctaaca tcgggtcagg gac
//
LOCUS       DAZSIO010000175         7336 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZSIO010000175 DAZSIO010000000
VERSION     DAZSIO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.177"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            344..1915
                     /gene="garD"
                     /locus_tag="ACHX5T_003479"
     CDS             344..1915
                     /gene="garD"
                     /locus_tag="ACHX5T_003479"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR8468519.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(1967..2791)
                     /gene="agaR"
                     /locus_tag="ACHX5T_003480"
     CDS             complement(1967..2791)
                     /gene="agaR"
                     /locus_tag="ACHX5T_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFR8468520.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            3040..4320
                     /gene="kbaZ"
                     /locus_tag="ACHX5T_003481"
     CDS             3040..4320
                     /gene="kbaZ"
                     /locus_tag="ACHX5T_003481"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFR8468521.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            4343..4816
                     /gene="agaV"
                     /locus_tag="ACHX5T_003482"
     CDS             4343..4816
                     /gene="agaV"
                     /locus_tag="ACHX5T_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFR8468522.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            4827..5606
                     /gene="agaW"
                     /locus_tag="ACHX5T_003483"
     CDS             4827..5606
                     /gene="agaW"
                     /locus_tag="ACHX5T_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFR8468523.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            5596..6405
                     /gene="agaE"
                     /locus_tag="ACHX5T_003484"
     CDS             5596..6405
                     /gene="agaE"
                     /locus_tag="ACHX5T_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFR8468524.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            6492..6926
                     /gene="agaF"
                     /locus_tag="ACHX5T_003485"
     CDS             6492..6926
                     /gene="agaF"
                     /locus_tag="ACHX5T_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFR8468525.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            6923..>7336
                     /locus_tag="ACHX5T_003486"
     CDS             6923..>7336
                     /locus_tag="ACHX5T_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR8468526.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         1754 a         1884 c         1939 g         1759 t
ORIGIN      
        1 tattattatg gggaaggtgt tatttataag tttcatttat gccgtaacga caatgaactc
       61 gggaattagt ataagcagcg cgagaataat aatcattgtg caaatgctaa tttaattaat
      121 actttttaaa tattattttg agcatatgca cataaggttg cgcgctaaag cacagatttg
      181 cgctttacct tgccgggcgg cactgcaatc cctaaaatga ttgacattga tcacatttct
      241 gcgtttaaac tcctgacatt cttatttcac ccaatgaaat catttatttt taaatgagac
      301 caggtcctca ttttaataac ccctggctgg agaatattgc acaatggcca acatcgaaat
      361 cagacaagaa acgccaactg cgttttatat aaaagttcac gacacagata atgtggcaat
      421 tattgttaat gataatggcc tgaaagcagg aacgcgtttt ccggatgggc tggaattaat
      481 tgaacatatt ccccaggggc ataaagtcgc attgctggac attccggcta atggtgaaat
      541 tattcgttat ggcgaagtga ttggttacgc cgtgcgtgca atcccacgcg gaagctggat
      601 cgacgaatca atggttgtac taccggaagc gccgccgtta cacacgctgc cactggcaac
      661 caaagtcccg gaacccttac cgccgctgga aggatacacc tttgagggct atcgcaatgc
      721 cgatggcagc gtgggcacca aaaacctgct cggcatcacc accagcgtcc actgtgtggc
      781 aggcgtggtg gactatgtag taaaaatcat tgaacgcgat ctgctaccga aatacccgaa
      841 cgtcgatggc gtggtggggc tgaatcattt gtacggttgt ggcgtggcga ttaacgcacc
      901 ggcagcagtt gtacctattc gtaccattca caatatttcg ctgaacccta actttggcgg
      961 cgaagtaatg gtgattggcc tgggttgtga aaagttgcag cctgagcgcc tgctgactgg
     1021 aacggatgat gtgcaagcta ttccagtaga aagcgccagc attgtcagtt tgcaggatga
     1081 aaagcatgtc ggttttcagt ccatggtcga ggatattttg caggtcgccg aacgccatct
     1141 acaaaaactg aatcaacgtc agcgagaaac ctgtccggct tcagaactgg ttgtcggcat
     1201 gcattgcggt ggcagcgatg cgttttctgg cgtaacggca aacccggcgg ttggctatgc
     1261 gtctgatcta ctggtgcgct gcggcgcaac ggtgatgttt tcagaagtca cggaagtgcg
     1321 tgacgcgatc catctgctga caccacgcgc agtgaacgaa gaggtcggca aacggctgct
     1381 ggaggagatg gagtggtacg ataactatct caatatggga aaaaccgacc gcagcgccaa
     1441 cccttcgccg ggcaacaaga aaggcggtct agcaaacgtg gtagagaaag cgctcggctc
     1501 cattgctaaa tcgggcaaaa gtgcaattgt tgaagtgctg tcgcccggtc aacgcccgac
     1561 taaacgcgga ttaatttacg ccgcgacgcc agccagcgat tttgtctgtg gcacgcaaca
     1621 ggtggcttcg ggtatcaccg tgcaagtgtt tacgaccggt cgtggtacgc cgtacggcct
     1681 gatggcggta cccgtcatta aaatggcacc ccgcaccgag ctggcgaacc gctggtttga
     1741 tttaatggat attaatgcgg gcaccatcgc taccggcgaa gaaactattg aagaggaggg
     1801 ctggaagttg ttccacttta ttctcgacgt cgccagcggg aagaagaaaa ccttctcgga
     1861 tcaatgggga ctgcataacc agctggcggt gtttaaccca gcaccggtga cctgataatt
     1921 caattcgtat tatgcccgat agcacacgtt tatcgggcat attgagtcac agcgaagacg
     1981 cgtgatcccc gaccagaatc acttcaaccc cagcctttcg cagtccttcc agactatccg
     2041 caggaatgcc ttcatcaaca atgatcatgt cgatacgttg agtatcaatg atcttatgta
     2101 aactggaacg attgaattta ctggaatcgg tgaccacgat gatccgttcc gcaacttcgc
     2161 acatccgacg gcttaaacgg gcttcatctt cattatgtgt gctgacgccg cgctccagat
     2221 cgatcgcatc tacaccaaga aacagcatat cgaagtggta attttgcagc gattgctcag
     2281 cctgatcgcc gtaaaaagat tgcgactgac ggcgcaaatg cccgccggtc atcagcagct
     2341 caacgccttc cgcttccagc aacgcattag ccacgttcat accgttggtc atcgcaatta
     2401 cgtcagtgta cttgcgcatc agacgagcaa tctcaaaagt ggtggtcccg gaatcgagga
     2461 tcacccgatg acctggctga atcaactcaa cggcagcttt cgcaacgctg cgtttcatcg
     2521 cggtgttcag tgcgctttta tcttccactg atggctcgac tgacggcgtc gtgctatcgc
     2581 agatcaacgc gccaccatag gcacgcacag cgatcccctg cttttccaga aacgccagat
     2641 cattgcggat cgtcacagta gatacgccat acaatgccga cagatcgtta acctgcacac
     2701 tcccttgctg tcgcagacgc tgaatgatct gttctcgtcg ctcgctggtg cctgtcactc
     2761 gcttctcacc tgaagcgtcg gtattactca tagtaagtcc tttcgtaaaa ctttcgtttc
     2821 atttcgtttt gcctattaac gcctttctat taagcaaatg caagcccacc ttgcccattg
     2881 gcgcaagcta ctctcgtttc agtgactttc attacgtttc ttttgtgaat cagatcagaa
     2941 aactattatc tttcgtttta tttttatctc accatgacgc agtatcaatt gaaacaaaac
     3001 gaaagatcaa tatcgcagta atctgaactg gagaggaaag tgaaacatct gacagaaatg
     3061 gtgagacagc acaaagcggg caaaacaaat gcaatttatg ccgtttgttc cgcacatccg
     3121 ctggtgctgg aggctgcaat ccgctacgcc agtgcaaacc aaacgccgtt actgattgaa
     3181 gcaacctcca atcaggtaga ccagttcggc ggttataccg gaatgacgcc cgccgatttt
     3241 cgcggctttg tttgtcagct cgccgactcg ttgaatttcc cgcaggatgc gctgattctg
     3301 ggtggtgacc atctggggcc aaaccgctgg caaaacctgc cagccgctca ggcaatggcc
     3361 aatgccgatg atttgattaa aagctacgtt gcggcaggat tcaaaaaaat ccaccttgat
     3421 tgcagcatgt cctgtcagga cgatccaatt cccttaactg atgacatcgt ggctgaacgc
     3481 gccgcccgtc tggcgaaagt ggcggaagaa acctgtcgcg aacactttgg cgaagccgat
     3541 ctggagtatg tcattggtac cgaagtgccg gtacctggcg gcgcgcatga aaccttaagc
     3601 gagctggcgg tcaccacgcc ggatgccgcc cgcgccacgc tggaagccca tcgtcacgcc
     3661 tttgaaaagc aaggtttgaa tgccatctgg ccacgcatta ttgccctggt ggttcaaccc
     3721 ggcgtcgaat tcgatcacac caacgttatt gattatcagc ccgccaaagc gagcgcctta
     3781 agccagatgg tcgaaaacta cgaaacgctg attttcgaag cgcactctac cgattatcaa
     3841 acgccgcaat ctctgcgcca gctggtgatt gaccactttg ccattctgaa agttggcccg
     3901 gcgctgacct tcgccctgcg tgaagccctt ttttctctgg cggcgattga agaagaactg
     3961 gtaccagcga aagcctgttc cggtctgcgt caggtgctgg aaaacgtgat gctcgaccgc
     4021 ccggaatact ggcaaagcca ctaccacggt gacggcaacg cgcgtcgtct ggcgcgtggt
     4081 tacagctact cagatcgcgt gcgctattac tggccggaca gccagattga tgacgctttc
     4141 gctcatctgg ttcgtaatct ggcggattca ccaattccgc taccgctcat cagccagtat
     4201 ctgccgctgc agtacgtgaa agttcgctcc ggcgagctgc aaccgacgcc acgggaactc
     4261 attataaacc atattcagga catcctggcg cagtaccaca cagcctgtga aggccaataa
     4321 gcaaaacaaa gaggaacatg ctatgccaaa tattgtttta agccggattg atgaacgctt
     4381 gattcacggt caggtcggcg ttcagtgggt cggatttgcg ggggcaaatc tggtgctggt
     4441 agccaacgat gaggttgccg aagatccggt acaacaaaac ctgatggaaa tggtactggc
     4501 agaagggatc gccgtacgtt tctggacgct gcaaaaagtt atcgacaaca ttcatcgcgc
     4561 cgccgatcga cagaaaatcc tgctggtttg taaaacaccc gccgatttcc tgacgctggt
     4621 gaaaggtggc gttccggtga atcgcattaa cgttggcaat atgcactacg ccaatggcaa
     4681 acaacaaatc gccaaaacgg tttctgtgga tgcgggcgat atcgcagcat ttaacgacct
     4741 gaaagccgct ggggtggaat gcttcgttca gggcgtcccg acagagcctg ctgtggacct
     4801 ctttaaatta ctttgaggga ttcatcatgg aaatcagcct gttgcaggca tttgcgttgg
     4861 gcattatcgc ctttatcgct ggcctagata tgtttaacgg cttaacccat atgcaccgcc
     4921 cggtggtcct cggcccgttg gtcgggctgg tacttggcga tctgcatacc ggaattttaa
     4981 ccggcgatac gctggaacta gtgtggatgg ggctggcccc gctggcgggc gcacagccgc
     5041 ctaacgtgat tatcggtact atcgtcggca cggcgtttgc cattactact ggcgtgaaac
     5101 ccgatgtcgc ggtcggtgtc gccgtacctt tcgctgtcgc agtacagatg gggattacct
     5161 tcctgttctc ggtgatgtct ggcgtgatgt ctcgctgcga ccggatggcg gagaacgccg
     5221 atactcgcgg cattgaacgc gtgaactatc tggcactgct ggcactcggc actttctatt
     5281 ttctctgcgc tttcctgcct atctacttcg gcgcggaaca tgcaaaaacc atcattgatg
     5341 tcctgccgca acgattaatc gacggcctcg gcgtcgcggg cggcatcatg ccagcaatcg
     5401 gttttgccgt gctgctgaaa atcatgatga aaaacgtcta cataccctac ttcatcctgg
     5461 gctttgttgc cgcaacctgg ctcaagttac cggtgctggc tattgctgcc gccgcgctgg
     5521 cgatggcgct gatcgatctg ctgcgtaaat ctcctgaacc gactcaacct gcggcacaga
     5581 aagaggaatt tgaagatggc atctaatcaa accaccctgc cgaacgtcag cgaaaatgaa
     5641 gaaacactgc tgactggcgt caatgaaaac gtgtatgaag atcggagcat tgccgcggag
     5701 ctgacgaaaa aagatatcaa ccgcgtcgcc tggcgttcca tgctgttaca agcgtctttt
     5761 aactacgaac gtatgcaggc ttctggctgg ttgtacggtc tgctgcctgc tctgaaaaag
     5821 atccacacta ataaacgcga cctggcgcgc gccatgaagg ggcatatggg tttcttcaat
     5881 acccatccgt ttctggtgac gtttgttatc ggcattatcc ttgcgatgga gcgttctaag
     5941 caggacgtta acagtattca gagcaccaaa attgccgtcg gtgcgccgct tggcgggatt
     6001 ggcgatgcga tgttctggct aacgctactg ccgatttgtg gcgggatagg cgcaagcctc
     6061 gcgctacaag gctctattct tggcgcagtc gtctttattg tgctgttcaa cgtggtacac
     6121 ttggggctgc gttttggtct ggcgcattat gcttaccgca tgggcgtggc ggcgattccc
     6181 cttattaaag cgaataccaa aaaagtcggc catgctgcgt caatcgttgg aatgacggta
     6241 atcggcgcac tggtggcaac ctatgttcgt ttaagcacca cgctggaaat cacggcgggc
     6301 gacgcagtag ttaagttaca ggctgatgtt atcgacaaac tgatgcctgc cttcttaccg
     6361 ctggtctaca ccctgaccat gttctggctg gtacgccgcg gctagagtcc gctgcgcctg
     6421 attgcggtta ccgtggttct agggatcgtc ggtaaattct gccatttcct ttaaatacaa
     6481 tgaggtatta gatgttaagt attattttga cagggcatgg cgggtttgcc agtggcatgg
     6541 aaaaagcaat gaagcaaatc ctcggcgatc aatcgcagtt tattgccatc gattttcccg
     6601 aaacctcaag cgccgcgctg cttacatcgc agcttgaaga ggccatcgcc caactggatt
     6661 gtgaagacgg cattgttttt ctgacggatt tgctgggcgg cacgccgttt cgcgtagctt
     6721 caacgctggc gatgcaaaaa ccgggctgtg aagtgattac cggcaccaat ttgcaactgc
     6781 tactggagat ggtgctggag cgcgaagggt taagcggtga agaattccgt gtacaggcgc
     6841 tggagtgtgg acaccgaggg ctgaccagtc tggtggacga gttaggccgc tgccatgaag
     6901 aatgtccggt cgaggaagga atatgacaca cgttctgcgc gccagaaggc tgctgactga
     6961 agagggatgg ttcgatgacc atcagttgcg tgttgctgac ggtgtcatcg cagcaatcga
     7021 accgattcca gtgggcgtga ctgaacgcga tgcggaacgg ctctgccccg cttacatcga
     7081 cacccatgta cacggtggtg cgggcgttga tgtaatggat gacgcgccgg atgtactcga
     7141 caagctggca atgcacaagg cacgcgaagg tgtcggcagt tggttaccga ccaccgtaac
     7201 cgcgccgctt aataccattc atgcggcgct gaaacgtatt gctcaacgtt gccaacgcgg
     7261 cggacctggt gcgcaagtgc tggggagtta tctcgaagga ccgtacttca cgccgcagaa
     7321 taaaggcgcg catccg
//
LOCUS       DAZSIO010000176         7310 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZSIO010000176 DAZSIO010000000
VERSION     DAZSIO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.178"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..190)
                     /locus_tag="ACHX5T_003487"
     CDS             complement(<1..190)
                     /locus_tag="ACHX5T_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR8468527.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="ACHX5T_003488"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="ACHX5T_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR8468528.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="ACHX5T_003489"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="ACHX5T_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR8468529.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="ACHX5T_003490"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="ACHX5T_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR8468530.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="ACHX5T_003491"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="ACHX5T_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="ACHX5T_003492"
     CDS             complement(3262..3381)
                     /locus_tag="ACHX5T_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR8468531.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /gene="ymgJ"
                     /locus_tag="ACHX5T_003493"
     CDS             complement(3342..3527)
                     /gene="ymgJ"
                     /locus_tag="ACHX5T_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR8468532.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /gene="ymgI"
                     /locus_tag="ACHX5T_003494"
     CDS             3628..3801
                     /gene="ymgI"
                     /locus_tag="ACHX5T_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR8468533.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="ACHX5T_003495"
     CDS             3803..4147
                     /locus_tag="ACHX5T_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR8468534.1"
                     /translation="MKKKILAFGLISALFCSTPAIADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="ACHX5T_003496"
     CDS             4151..4486
                     /locus_tag="ACHX5T_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR8468535.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="ACHX5T_003497"
     CDS             complement(4543..6864)
                     /locus_tag="ACHX5T_003497"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR8468536.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="ACHX5T_003498"
     CDS             complement(6981..7190)
                     /locus_tag="ACHX5T_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468537.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
BASE COUNT         2003 a         1731 c         1623 g         1953 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ctggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatagcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttagtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattatcaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatc
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa
     7261 acggagggta aagacaaaaa caaaagcaca aaaaatttaa atagataaac
//
LOCUS       DAZSIO010000177         7232 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZSIO010000177 DAZSIO010000000
VERSION     DAZSIO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7232
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.179"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="ACHX5T_003499"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="ACHX5T_003499"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR8468538.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2667)
                     /locus_tag="ACHX5T_003500"
     CDS             complement(1672..2667)
                     /locus_tag="ACHX5T_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR8468539.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="ACHX5T_003501"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="ACHX5T_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHX5T_003502"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHX5T_003502"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR8468540.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHX5T_003503"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHX5T_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR8468541.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLILCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..>7232)
                     /gene="emrK"
                     /locus_tag="ACHX5T_003504"
     CDS             complement(6108..>7232)
                     /gene="emrK"
                     /locus_tag="ACHX5T_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR8468542.1"
                     /translation="KKYFSLLVIVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPI
                     SAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQD
                     KQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQA
                     YKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGYIAQRSVQVGETVS
                     SGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGT
                     GNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKDEDIAE
                     MPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         2001 a         1714 c         1665 g         1852 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gctaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatat caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tt
//
LOCUS       DAZSIO010000178         7184 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZSIO010000178 DAZSIO010000000
VERSION     DAZSIO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.180"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(371..1198)
                     /locus_tag="ACHX5T_003505"
                     /pseudo
     CDS             complement(371..1198)
                     /locus_tag="ACHX5T_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACHX5T_003506"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACHX5T_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFR8468543.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="ACHX5T_003507"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="ACHX5T_003507"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFR8468544.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="ACHX5T_003508"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="ACHX5T_003508"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFR8468545.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="ACHX5T_003509"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="ACHX5T_003509"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFR8468546.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="ACHX5T_003510"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="ACHX5T_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7184
                     /locus_tag="ACHX5T_003511"
     CDS             7090..>7184
                     /locus_tag="ACHX5T_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HFR8468547.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGAL"
BASE COUNT         1676 a         1812 c         1876 g         1820 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gtattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgct
//
LOCUS       DAZSIO010000179         7153 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZSIO010000179 DAZSIO010000000
VERSION     DAZSIO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.181"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..164)
                     /locus_tag="ACHX5T_003512"
     CDS             complement(<1..164)
                     /locus_tag="ACHX5T_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468548.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLT"
     gene            242..1336
                     /gene="ddlA"
                     /locus_tag="ACHX5T_003513"
     CDS             242..1336
                     /gene="ddlA"
                     /locus_tag="ACHX5T_003513"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR8468549.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1360..1572)
                     /locus_tag="ACHX5T_003514"
     CDS             complement(1360..1572)
                     /locus_tag="ACHX5T_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR8468550.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1832..2140
                     /locus_tag="ACHX5T_003515"
     CDS             1832..2140
                     /locus_tag="ACHX5T_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR8468551.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2199..3293)
                     /gene="yaiW"
                     /locus_tag="ACHX5T_003516"
                     /pseudo
     CDS             complement(2199..3293)
                     /gene="yaiW"
                     /locus_tag="ACHX5T_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
     gene            complement(3306..4526)
                     /gene="sbmA"
                     /locus_tag="ACHX5T_003517"
     CDS             complement(3306..4526)
                     /gene="sbmA"
                     /locus_tag="ACHX5T_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR8468552.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4878..6035
                     /gene="ampH"
                     /locus_tag="ACHX5T_003518"
     CDS             4878..6035
                     /gene="ampH"
                     /locus_tag="ACHX5T_003518"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR8468553.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6036..6539)
                     /locus_tag="ACHX5T_003519"
     CDS             complement(6036..6539)
                     /locus_tag="ACHX5T_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8468554.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(6627..>7153)
                     /locus_tag="ACHX5T_003520"
     CDS             complement(6627..>7153)
                     /locus_tag="ACHX5T_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR8468555.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         1815 a         1827 c         1727 g         1784 t
ORIGIN      
        1 ttcgttaact tataattaag agaaaaaaac tcaccgcatt gccatttaag ccaggaataa
       61 attagcttcc cgttcatcat ttttgtgatc ttgcgcacat ctttgagata acgcactttc
      121 ttcaccagta cgttgcacgc tttgtcagca gaagattgca acaatctttc agaaggccgc
      181 gaatcccccg ttttgtcgtg gtaatgtatg cactttgtgt tagacgggat aggtttttaa
      241 gatggaaaaa ctgcgggtag gaatcgtttt tggtggtaaa tcagcggaac atgaagtgtc
      301 tctgcaatcg gcaaaaaaca ttgtcgatgc cattgataaa agtcgcttcg acgttgtgct
      361 gctgggcatt gataaacaag ggcaatggca cgtcagcgat gccagcaatt atctgctaaa
      421 tgcagacgat cctgcccata ttgcgttgcg cccttcggcg accagccttg cgcaggtgcc
      481 aggtaaacat gagcatcagc ttatcgacgc gcaaaacggt cagccgttgc cgacggtgga
      541 tgtcattttc ccgattgtcc acggtacgct gggcgaagat ggttccttgc agggaatgct
      601 gcgggtcgcc aatttaccgt ttgtaggttc tgatgttctg gcttcagcag cctgtatgga
      661 taaagatgtc accaaacgtc tgctacgcga tgccgggctg aacattgcgc catttattac
      721 cctgacgcgc gctaatcgtc acaacatcag ttttgccgaa gtggagtcta aactggggtt
      781 accactgttt gtaaaaccgg ctaatcaggg ctcttctgtt ggtgtcagca aagtaaccag
      841 tgaagaacag tacgcaattg ccgtcgatct ggcgttcgag ttcgatcata aagtgatcgt
      901 tgagcaaggg atcaaaggtc gtgagatcga atgcgcagtt ctgggcaacg acaatccgca
      961 agccagcacc tgtggcgaga tcgtactcac cagcgatttc tatgcctacg acaccaagta
     1021 cattgacgaa gatggcgcga aagtggtagt tccggcagcc attgcaccag agatcaacga
     1081 taagatccgg gcgattgccg ttcaggctta tcagacgttg ggatgcgcag gcatggcgcg
     1141 tgtagacgtg tttttaaccc cagagaacga agtggtgatc aacgagatca acacactgcc
     1201 tggcttcacc aacatcagta tgtatccgaa gctgtggcaa gccagcggtc tgggttacac
     1261 cgatctgatc acacgtttga ttgagctggc gctggagcgt cacgctgcgg ataacgcatt
     1321 gaaaaccaca atgtaatatt ttaacgcccg ataatattct tattgggcgt tttcagtttc
     1381 ttcatcgcga cgacgaataa tcaaccccgc cagccagaaa ctaatcaccc acgtcaccag
     1441 accgacggca taggtctgcc aaccttttgc ttcaaatccc agtaccccca ccacgccatt
     1501 aagaatgaat ataaggccaa tggcgaacgc atagtagtgc cagtcacggc ggatttttac
     1561 aggcagattc atggcagctc cgttaacgaa aaacaacatc cttgcatatt ctgtaacaac
     1621 cgtctgtggt gtagacaaca ttttcgtaat tattaataaa tttaacttaa ggttgccatt
     1681 ttgtgactca caacgaagat tcacgcttcg ggaatattcc caacatcaat ttaccatgat
     1741 tctgatattg acaatctaaa tgacctgtca gacttgccat aagccgctgg tatcacacca
     1801 gaagagaagc agatgacttc cggaggtttt tatggctgat ttcaccctgt caaaatcgct
     1861 gtttagcgga aaatatcgca atgcctcttc aacgcctggc aacattgcct atgcgttgtt
     1921 tgtgctgttt tgcttttggg ctggggcgca attgctgaac ctgttagtgc atgcgcccgg
     1981 cgtctatgag cgtttaatgc aggtccagga aacaggtcgc ccacgggtgg aaattggttt
     2041 aggtgtcggc accattttcg ggctgatccc gtttttagca ggctgcctca tttttgcagt
     2101 ggtggcgcta tggctgcact ggcgacatcg ccgccagtaa aatttacgtt gccagatgcg
     2161 gcctgaaacg ccttatctgg cctgacattc ctgtttgatc actgtttcat acaacgcgcc
     2221 cgccgttcgt ctacgcgctt cgcaaaccag gccgtagtca ggttgcgggt gattttcggg
     2281 ctttccagtt gaatgccagg caacatttcg cggggtagtg atttacccgt tttcgcttcg
     2341 gcaagttgat aaactttctt gtacagcgcc gtctcctcaa aagagaagct gtcacctttc
     2401 tctaactgac ggcgaatttc gctgtcgctc atccccagtt ttcccgccag tttgcgcgtt
     2461 gccagttcgg ttttcccggg ttctttactg tcgtaacgaa ttaaatcacc gtccagtgcc
     2521 agcttcacgc cgctggcctt actgactgcg ttctgaaacg cggcattacg gctggcgtac
     2581 cagccagcgt taaaatcagc aaagcggtat atcggtgcgc tatagctggc gggataattc
     2641 agtaaatggt aagtaccaaa ccacaacccg ccgcggcgac tgaagacttc ctgacgaact
     2701 gtaccgtcca ttttccacgg ataccctttg gtatgctgtt cggcaaaagc aatgctgacc
     2761 tgcatcggcc caccggtgcg caccggatta agcgaaccaa acagcgtctg ccccattggt
     2821 accatgctga tcagatcgtc aaaaatcgcg ctcaattgct tttctgtacg caccgaatct
     2881 aaccgttcgc tataactttt gccgtttggc gatttgattt ttagcgcggt atgaaccaga
     2941 aaagcaggaa tgtgcatccg ttcagcacga cggtcaatct cttgctaggc gatcttgctt
     3001 aagcccggaa cggccggatc tgcctgataa tttgactcct gctgcgccac cgccagcacc
     3061 gagtagacgt tttccagcgt cggtgccagc ccctgacttt caaaggtggt ggcgagatct
     3121 ttcgcccagg catcgcggtc tttcacgctc gcaggcatct tctgccgcac gacgctcgct
     3181 acatctatcg ctttttcgcc ctttttcaac ggttgcggag cctgactact acaccccgct
     3241 aaaaccagaa ccgccaacaa tgacagcgaa gatagatgat tgacgcgcga cattacttct
     3301 cctttttagc tcaaggtatg ggttacttcc tgaattttgt caccatccag ctcatgttca
     3361 aagctgcgca gacgtttgta gatagacatc aactcaacca gtgtggtcca tgagttaatc
     3421 aggtactgga aagcaccgcg aacctgacca aaaacgttgg taatctgcgt catcaggccg
     3481 agcgtaatcg taccggcaac aattgacgga aacagcaaga acaaaccgaa aacgttatcg
     3541 acctgcaaat agagaatgcg ggcgatgttg aaatacatat agtgaaaata gaggcgaaaa
     3601 tagtttttcc gtacggcgct aaacagctcg cgtaccgtag gcggcgtcgc gcgcgtggca
     3661 tcgtcttcac cataaaccag ctctttacgg taggcggcct ctactcgctg gtttttaaac
     3721 tccagccccg gcagtttgat ccctaccact gccagcaatc cggtccccat cagcgaccag
     3781 acgatagcgg caatcaccag accatacgga atgtgcccga taatcggcag ctccggcaca
     3841 tgcgcggaga gcgttaccag caccggcagg aaggcgatca acgtcatgat ggcgttgata
     3901 aaactgacgc ccatattctc cagcgttgaa gcaaaacgca tggtgtcttc ctgcacacgc
     3961 tgtgcggcac cttcgatatg acgcagttgt tgccagttcg ccatgtaata ttcgttcatc
     4021 gccgtacgcc agcggaacac gtagtgactg acaaagaagt tgttcagtac actgatcacc
     4081 accgcgatca gcgcaatccc cagaaagacg cccacttcgc ggtaaaattg ttcgatagtg
     4141 actttatgcg gcgaacttag cgcggtttga atcagatcat agaacggcgc ataccaggcg
     4201 ttgacggcga ccccgacttc caccaaaaac caagtgacga agataatcag cgcagtaccg
     4261 agtattgacc agtattgcca acggtgcggg ctgtagataa accagaaaaa tgcaaaaagt
     4321 cctacgcaaa caatgtagta agcgtaaaaa atcaggaaat ccaacgacca gaaacgcgcg
     4381 gcgctaatcg ggatctgccc ggaagcgccg gtgatacgtg ccacccagtc acccccaccg
     4441 gcttgccaga agataacggc aatcaatgcc caaacaaagg ccgagagaaa aaacgttccc
     4501 ggctttggga aaaaagactt aaacatatgc actcctgcta acttcttatc gttttaacaa
     4561 ctgctgtgta ttaaccgcat gaccgctaca aagcgtcagc caaatgaaac cgcccattgt
     4621 gacccatagc aaggcgctta gttcgccagc ctgaccataa gaattgtttc gacggatttc
     4681 acaaaccaaa gattgtttac ataagcggat taacacgcgc aggatcacaa atgcaggcat
     4741 tggtgagcag gaaaaagatc gtgtttacgc gccgttacgt ttttaacact attattgcac
     4801 taattcgccc tttgcaatcc atcaatgagt agtataaata cgctcagtta ccttcattca
     4861 atctatggac accaccgttg aaacgtagtc tgcttttttc tgccgtgctg tgtgcggcgt
     4921 cattgacctc cgtccatgcg gcgcagccga tcactgaacc ggagtttgcc tctgatattg
     4981 tcgatcgtta tgccgatcat attttttacg gcagcggtgc cacggggatg gcgctggttg
     5041 ttatcgacgg taaccagcgc gtctttcgta gttatggtga aacgcgacct ggtaataacg
     5101 ttcgcccgca gctggattcg gtcgtgcgta ttgcttccct caccaaacta atgaccagtg
     5161 aaatgctggt gaaattgctc gaccagggga ccgtaaagtt aaacgatccg ctaagtaaat
     5221 atgctccgcc aggcgcacgc gtgccgacct acaatgggac gcccattacc ctagtgaatc
     5281 tggcaaccca taccagcgcc ctgccccgtg aacagcccgg tggcgcggca catcgtccgg
     5341 tgtttgtctg gccaacgcgc gagcaacgct ggaaatacct ttctacggcg aagctgaaag
     5401 ccgcgccagg cagtcaggcg gcgtactcta accttgcgtt cgacctgctg gcagatgcac
     5461 tggcgaatgc gtccggcaag ccttataccc agttgtttga agagcagatt acccatccat
     5521 tggggatgaa agacaccacc tacaccccct caccggatca gtgccgccgt ttaatggttg
     5581 ctgagcgcgg tgccagtccg tgcaataaca cgctggcggc aattggcagc ggcggcgtct
     5641 attccacgcc tggcgatatg atgcgctgga tgcagcagta tttgtcatct gatttttatc
     5701 agcgcagtaa ccaggcagat cgcatgcaga cgttgattta tcagcgcgcg cagtttacga
     5761 aagtgattgg catggatgtc cccggcaaag cggatgcgct tggtttaggc tgggtatata
     5821 tggccccgaa agagggtcgt ccgaggatta ttcagaagac aggcggtggt ggcggtttca
     5881 ttacctatat ggcgatgatc ccgcagaaga atattggcgc gtttgtggtg gtcactcgtt
     5941 cgccgttgac gcgctttaaa aatatgagcg atggcatcaa tgatctggtg accgagctaa
     6001 gcgggaataa accgttggtt atccccgcat cctgattaat actctgaagg caaacggttg
     6061 atagcgacca gtttgccttt attcatttcg atatagccgc ctttacgcaa agcagcgaga
     6121 acttctgcga cgacagaacg cgatatgcgt gtacgttgat ggatataatt catcacacca
     6181 atacgtgatc gcaattcttc attccagtca atcattgata ataatgttgc gcgggtttgt
     6241 ttggctggta gatgatatcc cgtacaattt ccttctgata ttaatttgta tggtatatcg
     6301 tttttcatta aaccatcagc cagccccata atataaggag cctgggtaat accgataagt
     6361 acgttttctt ctctacgcag agagataact ccttctaaaa taacgaaagt atcttcgtta
     6421 ttgacatcac tggaaaatat aacttgcttt tcattattaa actcgaagcg cgtaccgtat
     6481 ctggacaaac atttatcgag cttaccaaat tcctgaagag gtttaactac agataacatt
     6541 tgcgcgtcct ttgcagtaat gcccgtcaaa tccttgacgg gcattattta gattaaatta
     6601 ccagtatttc ttcggagtga agaatattac caagtatatt taacacccac gttcgcggac
     6661 cagtcttgat ctacgtcacc accaccgagg tagttagcat cggtataggc gctgaagttc
     6721 ttggtgaagc tgaactgagt acccagacca acacgtaccg cagacccttc agtaccgtta
     6781 tcgatggaat caccgttcac atcgttatcg ttgttagagt cgtcgtagac gtaagccagt
     6841 ttgaagtacg gagtcagagc ctgatcttcg ctgtaggtga aggtataacc tgcatctaca
     6901 cccagttcat aacgcatgct gtcgtaaggc tgaccgtcaa ctttcatgtc gttgctcagc
     6961 tggtagtcat caccagactg gaacagacca gaaatgctgc cgtaaggagt cacgtaacca
     7021 gcatcaccca gtttgaagtc gtaaccggtt ttcaaaccga agccccaagc gtcggagttg
     7081 gtgctaccgt caacgtaagt accgttgctc atggttgcag acaggtcgtt gttgaagtga
     7141 gagtagctca agc
//
LOCUS       DAZSIO010000180         7058 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZSIO010000180 DAZSIO010000000
VERSION     DAZSIO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.182"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(437..922)
                     /gene="sixA"
                     /locus_tag="ACHX5T_003521"
     CDS             complement(437..922)
                     /gene="sixA"
                     /locus_tag="ACHX5T_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR8468556.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(1125..3269)
                     /gene="fadJ"
                     /locus_tag="ACHX5T_003522"
     CDS             complement(1125..3269)
                     /gene="fadJ"
                     /locus_tag="ACHX5T_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR8468557.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3269..4579)
                     /gene="fadI"
                     /locus_tag="ACHX5T_003523"
     CDS             complement(3269..4579)
                     /gene="fadI"
                     /locus_tag="ACHX5T_003523"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR8468558.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4759..5043)
                     /locus_tag="ACHX5T_003524"
     CDS             complement(4759..5043)
                     /locus_tag="ACHX5T_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR8468559.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5415..6755
                     /gene="fadL"
                     /locus_tag="ACHX5T_003525"
     CDS             5415..6755
                     /gene="fadL"
                     /locus_tag="ACHX5T_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR8468560.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1803 a         1857 c         1643 g         1755 t
ORIGIN      
        1 acatccttaa aaaatacgga ataagtattt agaaaaaata gaaagtatat caataatgaa
       61 tatcaacagc ttaaaaaaca aacaatgaac atataatgcg attggcattt atccactttt
      121 gacaatataa tatataaaca ctatagccag aatttaatca agtttcaatt acatcctgcc
      181 tgattattca actctatttt ctctattatc tgtattaata aatgacaatg agtgtcaaac
      241 agatattatt catttcaaca tgtaactttg ggcaaaatca agatacgcgt agaagaataa
      301 tttccgaatg aatgtagaaa gtgttttatt tatagataag gagagtcttc gatagtagtg
      361 aggttgacag atatccgctg cctaatgagc aattatgccc aatgaatcta cctcattggg
      421 cataatttgg aacacatcag atagcttttg ccatcttcag attacacggg ctcatctgcc
      481 agttgaatgt accgtttccg ctttcatcaa gcgttacgct ggcaatggcc gaggtggtaa
      541 acataggcgg cgtttcgccc ggacataact cggcaaccag atacccgact aacggtaagt
      601 gagagatcac cagtaccgaa gcaacacctt cattggtcaa cgcctgcaaa taggcactga
      661 ccagaccaac atcgccgcag ggcgttaact ccggcagaac ttctgcactg gaaggcaggt
      721 tcagacaatc ccctacttct tccagtgttt gctcggctcg caggaacggg ctcaccagaa
      781 cgcgttcgat ttccactttt tgacctttca gccagttcgc catcaggcga gattcgtcac
      841 aaccattagt ggtcagagga cgaaccgaat cactggcgga atcgagggct gcgtcgccgt
      901 gacgcatgat aaaaacttgc atattgcacc gcttttgtta accagattcg cccgttttct
      961 ttcatctgaa accagagaaa gaaaacggtg gccggcattg tgccttatcc attcaccgaa
     1021 tgaaacgctg ttttttacct caatggcgta agtatagtca atatgcgttt acattttgac
     1081 caacactccg ccattcagcg cggattcata tagctttgac cttcttattg caggtcagtt
     1141 gcagttgttt tccaaaaact ttccccacgc gcgcccatct cgaccaaacg ctcgcaaggg
     1201 gtaaaacggg aaccatactg cgtggccagt cgttgcatta ttgcaaccac ttcgcccgcg
     1261 ccgagagaat cgatatagcg gaacggtcca ccgagaaatg gcggaaaacc aatgccaaat
     1321 accgcaccaa tatccccgtc acgcacgcta cggataacct gctcatcaac acaacgtact
     1381 gcttcattca gcatcaacat cacacaccgt tcagcaacct gcggtgcgga gagtcgcccc
     1441 tgcccttgtg cgccaatgag cgggtaaatg gcaggatcga cctgtttttt gcttttacgc
     1501 cctttctgac cataaagata gaaaccccga ccattttttc tgcctttgcg atcgtcgttc
     1561 aaaattgaag aaacaacatt tgcaggcgcg ctaaaacgtt ctccataagc ggcttccagt
     1621 acaggaataa ttttagtccc ggtgtcgatt cctacctcat ccaaaagttg gattgggcct
     1681 accggaaaac caaatttcac tagcgcggca tcaatgtgct ctacccgttc accttcggtc
     1741 agcatgcgga tagcttcatt aatgtaaggc gctaagatgc gattgacgta aaacccggct
     1801 ttgtcacgca cgacaatggg cgttttgccc tgttttttcg ccagttttac cgtagtggcg
     1861 atggtttgcg ccgatgtacc cgcatgagga ataatctcta ccagcggcat tttttccacc
     1921 ggactgaaga aatgcaggcc aataacttgc tcaggtcgcg cggcgtgagc ggcgatatca
     1981 ccaatcggta aagatgacgt gttcgaagca aaaatggtat gagcggcgca attttgctcc
     2041 acttccgcca ccatctgttg ttttaattcg agattttcaa acaccgcttc aataatcaga
     2101 tcgcgatggg caaagccgcg atagtccgtc gttccggaga ttaatgccag ctgtttgtca
     2161 cgttcgctgg ctttgagatg acgacggcga actttgccct ccagctgatc ccaactgtac
     2221 ttcagcgcat gatttatgcc ctgcgggttg atatctttaa ttctgaccgg aagccccgct
     2281 ttacaagcag tgacataagc aataccgccg cccatcaagc caccacctaa aattcccacg
     2341 ctgtttaatg gcgcaggcgg tgcatcacta ccgggatctt tcttcacgtc cgtactggca
     2401 aaaaagatac tacgcaaagc ttgcgattgc ggcgtcatcg ccagttcgcc aaaggcgcga
     2461 gcttcagcgt catagccgct gctggtgccc tgcgctaatc ccgtttcaac aacctccagg
     2521 atgcgttctg tcgccggata attgccttga gttttgtgtt ctgttttctt gccgaccatt
     2581 ttgaacagca gcgcacgacc taacggcccc gccagaatac gctcgcgtac aggtagaggg
     2641 cgggaagatg ggcgatcctt cttcgccagc tcaacagcgg cttccagcaa aatggagtgc
     2701 ggaacaacgt catccaccag ccccagcttt aatgcctgtt tcgcccgaag ttgttttccg
     2761 gtgagaatca tctctaatgc tgtactgacg cctatcagac gcggtaaacg ctgggtgccg
     2821 cctgaaccgg gtaacaatcc aagttgtact tcaggcaaac cgagcaccgt tttaggatcg
     2881 tcagtacaaa cgcgaccgtg gcacgccagc gccagctcca gcccaccgcc caggcaagcg
     2941 ccatgaatag ccgcgataac cggaatgggc aaagcatgaa tctccgccat caactgttgg
     3001 ccctgccgcg ccagtgcttc cgcttcttgc gccgttttgc agttgccaat catgttgatg
     3061 tctgctccgg cgataaaatt gtccggtttc gcggagataa acaccacgcc gcgcaactct
     3121 ttgttttcac ggagttgctt aataatggcg cgcacctgcg aggcaaattc cgccttcagg
     3181 gtattcattt tctcatccgg tacgtcgatg gtgatgatag caatgttgtc cagacgaaca
     3241 ttaagagtaa acgctgatgc catttccatt attccgcctc cagaaccatt gccgcgccaa
     3301 gcccaccggc agcacaggcg gtaactaaac caaatccacc gccgcgacgg cgaagttcat
     3361 gcaatgtctg ggtaatcatc cgcgcgccgg tcgccgcgaa gggatgtccg taagcaatcg
     3421 aaccgccaag cacgttaaat ttgctatcgt ccacttcgcc agtggcatgt gcacgcccca
     3481 gtacttcacg agcaaaacgt tcactaccca gcaactgaat attcgccagt gtctgagctg
     3541 caaaggcttc gtgcatatcg atcaatgtca gttcgctcat cgtcaaaccg gcacgttcca
     3601 gcgccagcgg tgttgaccag gctggaccga gcaacatgtc ctgccagaca tcaatcgcag
     3661 taaatgcgta gctgcgcaga taccccagcg gcaccagccc taattctttc gcccgggatt
     3721 cagtcatcag gatcaccgct gccgcgccat cggtcagcgg cgtactgttt gccgccgtta
     3781 ccgttccgtg tttgcgatca aacgccgggc gcagctttgc gtaatcggca agcgaggaat
     3841 taccgcgaat attgttgtct tcgacaagcg gttgtttata aggagggata aaggcagtca
     3901 tcacctcttc tttgagtttt ccgtctgacc atgcctgagc ggcacgctga tgcgaacggt
     3961 gcgctaatgc atcttgctgt tctcgggtga tgccgtaggt tttcgccatt tgttcggcgg
     4021 tgtcgcccat ccgcaagcca gtagaatatt ctgctaccgc aggcggtacg ggcattaagt
     4081 cgcgcaaacg caggcgagag aagagtttca ggcgctggct catggttcga gctttgttga
     4141 catcaaccag cacgtacgcc agttttttac tgacgccaat tggcaatacg gaagaggaat
     4201 ctgccccacc ggcaatcccc gctcggatag ttcccgccat caggctttct gcgacgtttg
     4261 caaccgcttg gaaactggta gcgcaagcgc ggctgacgct gtaagcatcg gtatgcacat
     4321 tcattccggt accgagaaca atttcacgcg caatgttggg ggcttcaggc atttgtacga
     4381 cctgaccaaa gaccagctgt tcaatcactt cagcggggat ctcgctgcgt gccagcagtt
     4441 cgcctaccac catcttccct aaatcaaccg tgggaatgcc atgaaaagcc gtcgcctggc
     4501 gggcaaaagg cgtacgtaaa ccgctaacaa tggcgatacg atcgccctgg cgggtaacca
     4561 gtggtaaaac ctgacccata acactcccct gtaaaaaaat aaataaagtg gtctgacctg
     4621 atcatagtct taaccatttt tttacattta gccaagcgga gaaaaaggaa agtgggagct
     4681 atgacacagt gaagacagaa gaaagaaaag aaaacgcccc tgccagcaac tgacaggggc
     4741 gtacgtaccc gagaggaatt aacgcagacc cagctggaag atcagcattt cagcttcgca
     4801 ggcaaaagtg aaatcgatat ccaggcgcac accgtcagac tcttcagtga aagtcgggga
     4861 gattttgcac ggttcggatt tcacgctacg ggatttttca gtcagcgccg ccagcgtttg
     4921 ttctgcttct gcgcggtttg caaacacgcg gctgtaagac gcggtgcagt cggagttgtc
     4981 cataatggtg ccaacatcca tacagcagca aaccggggtt tcatcagcac tacatttact
     5041 catcgttgat ttcctctgta tgtgcaccca aggtgccaga taaacgttgt ggatatttta
     5101 cgcttccgga aagtgctgct ccagttgtta attctgcaaa atcggataag tgaccgaaat
     5161 cacacttaaa aatgatctaa aacaaaattc acccgaatcc gcgagtgcgc aacctccaaa
     5221 ttttgccagc tggatcgcgt ttcttagatc atatttgaaa aaagatggaa acatacttgc
     5281 aacattctag ctggtccgac ctatactctc gccactggtc tgatttctaa gatgtacctc
     5341 agaccctaca cttcgcgctc ctgttacagc acgtaacata gtttgtataa aaataaatca
     5401 ttgaggttat ggtcatgagc cagaaaaccc tgtttacaaa gtctgctctc gcagtcgcag
     5461 tggcacttat ctccacccag gcctggtcgg caggctttca gttaaacgaa ttttcttcct
     5521 ctggcctggg ccgggcttat tcaggggaag gcgcaattgc cgatgatgca ggtaacgtta
     5581 gccgtaaccc cgcattgatt accatgtttg accgcccgac attttctgcg ggtgcggttt
     5641 atattgaccc ggatgtgaat atcagcggaa cgtctccgtc tggtcgtagc ctgaaagccg
     5701 ataacatcgc gcctacggca tgggttccga acatgcactt tgttgcaccg attaacgacc
     5761 aatttggttg gggcgcttct attacctcta actatggtct ggcaacagag tttaatgata
     5821 cttatgcagg cggctctgtc gggggtacaa ctgaccttga aaccatgaac ctgaacttaa
     5881 gcggtgcgta tcgcttaaat aatgcatgga gctttggtct tggtttcaac gccatctacg
     5941 ctcgcgcgaa aattgaacgt ttcgcaggcg atctggggca gttggttgct ggtcagatta
     6001 tgcaatctcc tgccgggcaa actcctcaag ggcaagcatt ggcagctacc gccaacggta
     6061 tcgacagtaa taccaaaatc gctcatctga acggtaacca gtggggcttt ggctggaacg
     6121 ccggaatcct gtatgaactg gataaaaata accgctatgc actgacctac cgttctgaag
     6181 tgaaaattga cttcaaaggt aactacagca gcgatcttaa tcgtgcgttt aataactacg
     6241 gtttgccaat tcctaccgcg acaggtggcg caacgcaatc gggttatctg acgctgaacc
     6301 tgcctgaaat gtgggaagtg tcaggttata accgtgttga tccacagtgg gcgattcact
     6361 atagcctggc ttacaccagc tggagtcagt tccagcagct gaaagcgacc tcaaccagtg
     6421 gcgacacgct gttccagaaa catgaaggct ttaaagatgc ttaccgcatc gcgttgggta
     6481 ccacttatta ctacgatgat aactggacct tccgtaccgg tatcgccttt gatgacagcc
     6541 cagttccggc tcagaatcgt tctatctcca ttccggacca ggaccgtttc tggctgagtg
     6601 caggtacgac ttacgcattt aataaagatg cttcagtcga cgttggtgtt tcttatatgc
     6661 acggtcagag cgtgaaaatt aacgaaggcc cataccagtt cgagtctgaa ggtaaagcct
     6721 ggctgttcgg tactaacttt aactacgcgt tctgataacg cgttcgcctg gataaagtca
     6781 cctgctatgc aggtgacttt aactccccca cttcaccgaa agtagtttcc tcattgcttg
     6841 cctcctgagt tttgcaaaca gcctgatggc agcttgccct ttcaaatcag taagtggtat
     6901 ccacagacaa acctgaaggg aaatgcatat tttcaagcgt tctgctcgtc cttctcaaag
     6961 agtttacttt tctgcatttc caggatactc aacccccctg gctattgtgc gcttatacac
     7021 tcaaattaaa gataggttct aaataaatga gcgttttt
//
LOCUS       DAZSIO010000181         7018 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZSIO010000181 DAZSIO010000000
VERSION     DAZSIO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.184"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..358
                     /locus_tag="ACHX5T_003526"
     CDS             <1..358
                     /locus_tag="ACHX5T_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468561.1"
                     /translation="MKPLLLANNSNEYPGLFSAIQHKQQNVVETVYLALSDHARLFGF
                     TAEDIMDFWQHKAPQKYSAFELAFEFGHRVIAELILNTLNKMAESFGFTDNPRYIAEK
                     NYMEALLKKASPHTVR"
     gene            complement(327..2064)
                     /gene="aceK"
                     /locus_tag="ACHX5T_003527"
                     /pseudo
     CDS             complement(327..2064)
                     /gene="aceK"
                     /locus_tag="ACHX5T_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(2248..3552)
                     /gene="aceA"
                     /locus_tag="ACHX5T_003528"
     CDS             complement(2248..3552)
                     /gene="aceA"
                     /locus_tag="ACHX5T_003528"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFR8468562.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(3582..5183)
                     /gene="aceB"
                     /locus_tag="ACHX5T_003529"
                     /pseudo
     CDS             complement(3582..5183)
                     /gene="aceB"
                     /locus_tag="ACHX5T_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(5452..6381)
                     /gene="metA"
                     /locus_tag="ACHX5T_003530"
     CDS             complement(5452..6381)
                     /gene="metA"
                     /locus_tag="ACHX5T_003530"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR8468563.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            6538..6981
                     /locus_tag="ACHX5T_003531"
     CDS             6538..6981
                     /locus_tag="ACHX5T_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR8468564.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         1691 a         1793 c         1815 g         1719 t
ORIGIN      
        1 tatgaagccc ctcctcctgg caaataattc taatgaatat cccggtttgt tttcagcgat
       61 acagcataaa caacaaaacg ttgtagagac ggtttatctt gctttatctg accatgcacg
      121 cctgtttgga tttaccgctg aagatattat ggatttttgg caacacaaag cgccacaaaa
      181 atactctgcc tttgagttgg cttttgaatt tggtcaccgg gttattgctg aattaatcct
      241 taatacatta aataagatgg ctgaaagctt tggctttacg gataaccctc gatacattgc
      301 ggagaaaaat tatatggaag ctttactcaa aaaagcatct ccccataccg tacgctaaat
      361 ctttgcctgc gccgatacgc ataaacatct tccacatgcc cttcacgtat gcggttttgt
      421 agtgcgcgcc agtaatcagc gcggaacagg tcggcgtgca tctcttcaaa cagcggacca
      481 atacgcgggt cggcgcatag ccagtggcga aactcttccg ggaaaacatc gcccggcgag
      541 acgctgtacc acggttcgct ggcaagttcg tcttccggat agcgcggcgg cgggatgtcg
      601 cggaaattca cttccgtcat gtagcaaatt tcatcgtaat cataaaaaac cacacgcccg
      661 tgacgggtga caccgaagtt tttaaacagc atgtcgccag ggaaaatgtt agcagcggca
      721 agctggcgaa tagcgttacc gtattcttca atggcgtcgc gcaactgctg accttccact
      781 tgttccagcc agatattgag cggcaccatc cgccgctcga ttatagagat ggcgaattac
      841 aatctgctca ccaagatcgg tgattttttc cgctgcttcc tggagcagta attccattaa
      901 taccggggaa atatgccgct tctccagcac aaagttttca aactcctggg tgtccgccat
      961 tcggcccaca cggtcgtgct ctttcaccag ttgatagcag gcacgaacgt gagcggcaga
     1021 catctctttc tgcggcgcga acttgtcttt gatgactttg aatacccgat caaagcccgg
     1081 cagcgtaaac accaacatca ccattccacg aatacccggc gcttcaatga actgctcatt
     1141 acagccctgt agataaacga gatattcgcg gtagctttcg gttttggcgt gcttctggca
     1201 gccgatagcc atatacaatt cagcagtggt tttacctggc agaatttccc gtagccactc
     1261 gaccagcgct gcgggcagcg gtgcataaac cataaaataa gaacgcgcaa agccaaaaac
     1321 aatgctcgct tcggcggtcg tcgtcaggca ggtatcaata aataactcgc cgtcgtccgt
     1381 ctggtggatc ggcagtaaga agggcaacgt gccggaaggc gtgatcagtt tgcccaccag
     1441 ccaggcggct ttattgcggt aaaacagttc gttcgccacc tgtaaatgac tttccgcgag
     1501 gttgtctgtc cccagcgttt ccgtcagatg gcgaatgatg taatgaatgt cacggctttt
     1561 attctgccag cgcaggcgca gcggcaggtc gctgataacg cgcatcagta gagattccca
     1621 gccgtgatcg gggtgaaagt ctttcgccag cgggcgggga atggtacgaa agcggcgctc
     1681 tggctgagag ctaaagataa aaagccgctc gggagtaagc gagcggtggt caaataaccg
     1741 acagtacacg gagttaaaaa agctctccgc aatctcgaag cgcgggtaat ccggcaacag
     1801 ccgcgtgtaa tgctctttaa cgcgcagtaa aaatgccgcg tccgtacttt ggccgttagt
     1861 aatgcagcgc agttgctcca cgaccagacc aacgtgatga tcgtaaagat ggatacggtt
     1921 tttcatcgcc tgctggacag catgccagtc ggcctgttcg aaacgctgct gcgccccgga
     1981 agtcacttcg aggaatcgac catactgagc gtcgaagcct tgcaaaatgg tttgagcaat
     2041 cagtaattcc aggccacgcg gcatttagcg ccctcatcag gagcagagaa ttgccggatg
     2101 cggcgtaaac gccttatcca gcctacgttc ggcaacggtt gtaggcatga taagacgcgc
     2161 aagcgtcgca tcaggcatcg tgcaccgatt gccggatgcg gcgtaaatgc cttatccggc
     2221 ctacagtcag caacgtttgt aggttgctta gaactgcgat tcttcagtgg agccagtcag
     2281 cgcggtgact gaagacgtgc cgccctgaat aatagtcgtc actttatcga agtaacctgt
     2341 acccacttcc tgctggtgag atacgaaggt atagccatct ttcgcggcgg caaattccgg
     2401 ctgctgcact ttctcaacat agtgcttcat accttcgccc tgcgcataag cgtttgccag
     2461 gtcgaacatg ttaaaccaca tgctgtggat acctgctagg gtgatgaact ggaacttgta
     2521 gcccatatcc gacagctgct gctggaagct ggcaatagtt ttgtcgtcga ggtttttctg
     2581 ccagttgaac gacggcgagc agttataagc cagcagtttg cccggatatt tcgcgtggat
     2641 agcttgtgca aagcgacgcg ccagttccag atccggcgtg gaggtttcac accagaccag
     2701 gtcagcatat ggcgcatacg ccaggccacg gctgatcgct tgctcaatgc ccgcatgagt
     2761 acggaagaag ccttcactgg tacgctcgcc ggtaataaat tcgctgtcat acgggtcgca
     2821 atctgaggtg atcagatccg ccgcatcagc atcggtacgt gcaaccagca gggttggaac
     2881 gcccgtcacg tcagctgcca gacgcgccgc gaccagtttc tgaatagctt cctgagttgg
     2941 cactaaaact ttgccgccca tgtgaccgca tttcttcact gacgccagct gatcttcgaa
     3001 gtgaactgcc gcagcaccgg cttcaatcat cgctttcatc agttcaaagg cattcaggac
     3061 accgccaaaa ccggcttccg catcggcaac gatcggcagg aagtaatcga catagcgcgg
     3121 atcgcccggc tcaatgccca cggaccattg gatctgatcg gcacgacgga aggtgttgtt
     3181 gatccgctcc accacagctg gcaccgagtt tgccggatag agcgactgat ccggatacat
     3241 gctggccgcc aggttagcgt ccgccgctac ctgccatccc gacagataga ctgcttcaat
     3301 acccgctttc gcctgttgca gcgcctgacc gccagtcagt gcaccgaggt tgttgatgta
     3361 gccttttttc gactcaccgt gcagcagacg ccacattttc gctgcgccca gttgcgccag
     3421 cgtgcattca ggattgactg aaccgcgtaa tttcaccaca tcttccgcac tgtatgggcg
     3481 agtaatgcct tcccaacgcg gttgagtcca ctctttctgt aattcttcaa tttgttgtgt
     3541 acgggttttc atgtgcagat gctccatagt tatgtggtgg tttacgctaa caggcggtag
     3601 cctggcaggg tcaggaaatc aattaactca tcggaagtgg tgatctgttc catcaagcgt
     3661 gcggcatcgt caaaacggcc ctgggagaaa cgttcttcgc ccagttcgct ggcaatgact
     3721 ttcatctctt cgcccagcat ctggcggaac aaggctttgg tcaccggttt gccattgctc
     3781 aacgtttttt gatgatggat ccactgccag atcgaggtac gggaaatttc agccgtcgcc
     3841 gcatcttcca tcaggccata aatcggcacg cagccgttgc cggagatcca cgcttcgatg
     3901 tactgcacag ccacgcgaat gttagcgcgc ataccttctt cggtgcgttc accatcacaa
     3961 ggtgccagca gctgatcggc agtaatcggc gcgtcttgtt cgcgcaatac ttcaagctga
     4021 tttttacggg agccgagaat gtcgttgaat accgccattg ccgtgtccgc aaggcctggg
     4081 tgagcgatcc atgtgccatc gtgaccgtta ttggcttcca gcgctttatc cgcttttact
     4141 ttgttgagca cctggttatt acgctcttca tctttgctcg gaataaacgc ggccatgccg
     4201 cccatcgcaa aagcaccgcg tttatggcag gttttaatca gcaggcgtga gtaagcattc
     4261 aggaatggtt tatccatcgt cacggcctgt ctgtctggca ggacacgatc gggatagttt
     4321 ttcaacgttt tgatatagct gaagatgtaa tcccagcgac cgcagttcag accaacaata
     4381 tggtcgcgca gcgcgtgaag gatttcgtcc atctggaaca cggcgggcag cgtttcaatc
     4441 agcaacgtcg ccttgatggt gccgcgcggc agattaaagc gatcttctgc atagctgaag
     4501 acttcgctcc accaggccgc ttcctgctag gactgggttt tcggcagata gaaataggga
     4561 ccgctgccct ttgccaacag tgcctgatag ttgtggaaga aatagagcgc aaaatcaaac
     4621 aggctaccgg ggattgcctc accacgccag gtgacatgtt tttccggcaa gtgcagaccg
     4681 cgtacccgac aaatcaaaac cgctggattg ggcttgagct gataaatttt gcctgcttca
     4741 ttggtatagc tgatggtacc gttaaccgca tcgcgcaggt taatttgccc gtcgatcact
     4801 ttgttccagt ctggtgccag tgaatcttcg aaatcggcca taaagacttt cacattggcg
     4861 ttcagtgcgt tgatcaccat cttgcgctct accgggccag ttatctctac gcggcggtct
     4921 tctaagtccg caggaatccc gcgaattttc caatcagcat cgcgaatgga agccgtttcc
     4981 gaaataaaat caggcaacgt tccgttatca atatcttgct gctgctgaat gcgcgctgcc
     5041 agaagtttat tgcgttgtgg cgtaaaatgc gtcaccagct cagtcagaaa ttctaccgct
     5101 tcggcagtaa gaatttgctt ctcctgctcg ccatacggcc ttgtgaaagc cagttcatcg
     5161 gttgttgttg cctgttcagt catcgtgcag ctcctcgtca tggatccgaa aacttcccct
     5221 ctgtgaacga aggaacgttg tgcgcttttc gttcagcaca actaagacta ctcatttaaa
     5281 atgcaaaatc aaaaacaatt tccattttaa ttaaaaatac ttgataactt attgataaca
     5341 atatgaataa aatttattca cttgctgagg tgcgtttcgg aaataaaaaa ggcacccgaa
     5401 ggtgcctgag gtaaggtgct gaatcgctta acgatcgact atcacagaag attaatccag
     5461 cgttggattc atgtgccgta gatcgtatgg cgtgatctga tagacgtaat agttgagcca
     5521 gttggtaaac agcaaattac cgtgactacg ccagctcgct cgcggtttgt tttgcggatc
     5581 gttgtgcggg aaatagttat acggtacatc cgggtctagt ccggcttcca catcgcgtaa
     5641 atattcctgc gccagcgttt gcgcatcata ttcgggatgg cccgtcacaa aggcaatgcg
     5701 cttatctttg ctggcaaaca gatatgcatc cccttcttcc gtctctgcca gaatttccag
     5761 atcggtgtaa tcacgaatca acgctgccgg aaagtcagca tagcgcgaat gcggtgccag
     5821 gaatgaatca tcaaagccac gcgtcagaag cgcatgagga tggagaatat gatgctcgta
     5881 aacgccagag agtttgtcgg tgcgagtttg cttaggaatg ccgtagagga tattgagcgc
     5941 ggcctgtacc gcccagcaga caaacagcgt cgaggtgacg tgatctttcg accactccag
     6001 cacctgtttg atctgcggcc agtaagcgac atcattaaac tccaccaggc ccagcggcgc
     6061 accggttaca atcagaccgt caaagttctg ttcctgaata tcttcaaagt tacagtagaa
     6121 gttgttcaga tgctctgcgg gcgtgttgcg cgattcacgg gaatcgatgc gcaacagctg
     6181 aatatcgacc tgcaaaggtg agtttgaaag caggcgcaga aactgatttt cagtttcaat
     6241 cttcttcggc atcaggttaa ggatcagaac cttaagtgga cgaatttcct gaccagacgc
     6301 acgagaagtt gtcatcacaa agacgttttc ttcacgcaag aaattgacgg cgggtagctc
     6361 gtccggcaca cgaatcggca taacctgatt acctcactac atacgcttat acgtttagac
     6421 atccagatag ctgaagataa ccagaaaatt tgccaatgtc gagcctgcat gttgaaggtg
     6481 agaaagtttc aaggaaaagg ctacgttaga atataagaat gacgaaaagg agagaaaatg
     6541 gttattagta ttcgccgctc acggcatgag gaaggggagg aactcgttgc gatttggtgt
     6601 cgatctgtcg atgccaccca cgatttttta tcagcagagt atcgggccga gctggaagag
     6661 ctggtgcgtt cctttctgcc ggaagcaccg ttgtgggtcg cggttaatga acgggagcag
     6721 ccggttgggt ttatgttgtt aagtgggcag catatggatg cgctgtttat cgatcctgat
     6781 gtgtgcggct gcggcgtggg tcggatgctg gtggagcatg cgctctcgat ggcaccggaa
     6841 ctgacaacca acgttaatga gcaaaatgag caggcggttg ggttctataa gaaggtgggt
     6901 tttaaggtta cgggacgctc tgaggtggac gatttgggga aaccgtatcc gttgctgaat
     6961 ctggcgtatg tgggggaata agccgcctct gccgtgcctg cccaagctgg agaagacg
//
LOCUS       DAZSIO010000182         6723 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZSIO010000182 DAZSIO010000000
VERSION     DAZSIO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.185"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..173)
                     /locus_tag="ACHX5T_003532"
     CDS             complement(<1..173)
                     /locus_tag="ACHX5T_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="HFR8468565.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVL"
     gene            complement(163..486)
                     /locus_tag="ACHX5T_003533"
     CDS             complement(163..486)
                     /locus_tag="ACHX5T_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFR8468566.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVLAALMAIGEELRRLRLAVREQG
                     ARDDC"
     gene            551..742
                     /locus_tag="ACHX5T_003534"
     CDS             551..742
                     /locus_tag="ACHX5T_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR8468567.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            1401..1559
                     /locus_tag="ACHX5T_003535"
     CDS             1401..1559
                     /locus_tag="ACHX5T_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR8468568.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGNWTRWIEI
                     LRVLEVVA"
     gene            2107..2805
                     /locus_tag="ACHX5T_003536"
     CDS             2107..2805
                     /locus_tag="ACHX5T_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468569.1"
                     /translation="MLQHKMNSSSYAKVHNVSSLEDIMSYHNDDVLLKFRKEWNVTPE
                     EADDIFNETKKFIWLASTCLTECYNIKVHEQLQIIDEMWHTFIQFTDAYTSFCEKYLG
                     AYLHHYPNTNDMLKNEIRHVNEHGITFQEYRFNEYKNQIEKIAFYLGHETVAKWYGDY
                     AVRYSIKNINTIRIPKESISSDSYIEKVKSITHLPAAEFVKIIMRKDVWNDNGSVCGC
                     SGKGCGAGCSCNSR"
     gene            2844..4436
                     /locus_tag="ACHX5T_003537"
     CDS             2844..4436
                     /locus_tag="ACHX5T_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR8468570.1"
                     /translation="MNFIEKYIITFNKCNLFFIILLSTISSFLFVLFGYLIKTVIDNK
                     DSIGEINSLLIFIACFLSIRFLMPAGYSISEYLTQKTNIELSVKLREQVIDNIQNSHQ
                     EHFLKKNKGELNKVIESMLSSASSLFYTICSDVVPLLIQMIGIIITICISVNTLIAIE
                     FIIIMAIYIIFVIKMTQRRFPMMKSVALSSKFASGRMFDMMHMYPMDKAFHTTDKSRK
                     RVIQAVNTHSEKQRKVNNEFFLFGISSAFLSVIFSSLIILSAYWMFLHGRASRGSIIM
                     LATFLFQVFLPLNRIGYLFRQIKMARTEIDLYCAEINDIKKTNYTDKQYLHVKDNICN
                     IEIYNKSFNHKLILTKGVVTFITGENGSGKTTIAKILSGNITTEENTIKINGIQQEKA
                     NSPFVNVLYVPQDLDLMPGTIEENILHYSGINNLNTIKKLLHRFKFDKPLDYEIKGYG
                     HNLSGGQKQKLGVSLTSGKNVDLIIFDEPTKGFDSLGIKIISDYIKEENKEKHIIVIS
                     HDEQLINNIPKAQIIDITKHEV"
     gene            4439..5332
                     /locus_tag="ACHX5T_003538"
     CDS             4439..5332
                     /locus_tag="ACHX5T_003538"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFR8468571.1"
                     /translation="MNNLIKKEIIEKFKKYNFQKYPFVFTDVNYKNLINWNDLNKLLE
                     KDILHYPRVRMANDNFPEIRGYKGFIRYTYSQTGDRTPHINRHQLYKCLRDGATLIVD
                     RCQSFFESVDESRLWLSKELECTCSANLYAAFTATPSFGLHFDNHDVIAVQIEGIKKW
                     KVYNPTYSYPLEDERSFDYLPPNTSPDYEFDITPGQAIYLPAGYWHNVTTQSKHSLHI
                     SFTVIRPRRLELFKTLFDELKNNPYMREPIEHGDSLSDKEKIKTIITNAINSFDIQFA
                     ENMLKAQSRTYRYKKIKLEHI"
     gene            complement(5357..5719)
                     /locus_tag="ACHX5T_003539"
     CDS             complement(5357..5719)
                     /locus_tag="ACHX5T_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HFR8468572.1"
                     /translation="MTNFKSDLNLIRKEWYNAFYSGNTEMLDYLEADWFISTNGQKVI
                     YKKHQLRKIDMRPARILPSIERYEYDVVMREFKGIACVSGKAEIKQNDGNILMAFIEN
                     WIKVNGGWKMQFISYEKI"
     gene            complement(5716..5955)
                     /locus_tag="ACHX5T_003540"
     CDS             complement(5716..5955)
                     /locus_tag="ACHX5T_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 family protein"
                     /protein_id="HFR8468573.1"
                     /translation="MKISDIEKNIYKGLAEFISQKIRDEHDICLGQFESEELLDEILK
                     RVIPVIYNVAIDDAIKSVRNVGDRLEEELDMRKVV"
     gene            complement(6559..>6723)
                     /locus_tag="ACHX5T_003541"
     CDS             complement(6559..>6723)
                     /locus_tag="ACHX5T_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="HFR8468574.1"
                     /translation="ALERADRTLEPVIQREQEIRDERLIQEREHELSLERERQLEIQE
                     RTQDGPSLGW"
BASE COUNT         2187 a         1458 c         1202 g         1876 t
ORIGIN      
        1 agaacacctg acgtgtattt cttcgcaaat ggcgtggcat cgatgagttc ccggacttct
       61 tccggattac cctgaagcac cgttgcccct tcccggtttc gctcccttcc cagcaggtaa
      121 tcaaccggac cactgccacc gccttttccc ctggcatgaa atttaacaat catcccgcgc
      181 gccctgttcc ctgacggcca gacgcagccg acgcagctcc tccccgatgg ccatcagcgc
      241 agccaacacc tgaacccgat caccggaaga ccactgcccg ctgttcacct tcctcgctgt
      301 ctgattcagg ttattcccga tggcggccag ctgacgcagt aacggcggtg ccagtgtcgg
      361 cagcttcccg gagcgtgcaa ccggctcacc caggcagacc cgccgcatcc ataccgccag
      421 ctgtttaccc tcacagcgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatggt
      481 gagcatcctc tcgcgtttca tcggtatcat taccccataa aacagaaatc ccccttacac
      541 ggaggcatca gtgactaaac aggaaaaaac cgcccttaac atggcccgtt ttatcagaag
      601 ccagacatta acgctgctgg agaaactcaa cgaactggac gcagatgaac aggccgatat
      661 ttgtgaatca cttcacgacc acgccgacga gctttaccgc agctgcctcg cacgcttcgg
      721 ggatgacggt gaaaacctct gacacataca gctcccggag acggtaacag cttgtctgtg
      781 agcggatgcc gggagcagac aagcccctca gggcgcgtca gggggtttcg gggcgaagcc
      841 ctgaaccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagtac
      901 gaattgtatg gaaagcgcac catgtccgat gtgaaatgcc gcacagatgc gtaaggagaa
      961 aatgcacgtt ccggcgctct tccgcttcct cgctcactga ctcgctacac tcggtcgttc
     1021 ggctgcggcg agcggtgtct gctcactcaa aagcggagat gcggttatcc acagaatcag
     1081 gggataacgc caaaagaaac atgtgagcaa aaaacgagaa ccaggaaaag gctgcgccgt
     1141 tggcgttttt ccataggctc cgcccccctg acgagcatca taaaaatcga cgctcaagtc
     1201 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1261 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1321 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcggctcg gtgtaggtcg
     1381 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1441 ccggtaacta tcgtcttgag tccaacccgg taagacacga caaagcgcca ctggcagcag
     1501 ccactggtaa ctggacaagg tggattgaga tattaagagt tcttgaagtg gtggcctaac
     1561 tgcggctaca ctagaaggac agtatttggt atctgtgctc caccaagcca gttacccggt
     1621 taagcagtcc ccaactgact taaccttcga ctaaaccgcc tccccaggcg gttttttcgt
     1681 ttactggcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttt
     1741 gagatccgcc cactcctgga acctacaaca catgtgtaaa acgtcaatgg agggggctat
     1801 tatcggactg caacacattt cgtacagcct ttacactcgg tgaattagcg gccctagatg
     1861 catccactca ttcaaccaac tcaattctct tctcttagag tgggaaaatt ttgttttcct
     1921 tgcagcccct acccgtaaaa actggctgca aaaaccttgt attactaatg gatcctataa
     1981 ccattaaaaa acttgattac tatctcattt atagaattag gcatattgac acaagcacaa
     2041 atttaacaat accataacaa atgataacta ttctcatcta cattcaaata tataattggg
     2101 ggtgttatgc tacaacataa gatgaacagc agttcttatg caaaagttca taatgttagc
     2161 tcattagaag atatcatgag ttatcacaat gatgatgttc ttctaaaatt tcgtaaagaa
     2221 tggaatgtaa caccagaaga agctgatgat atctttaatg aaacaaagaa attcatctgg
     2281 ctagcctcaa catgcctaac ggaatgctac aatataaaag ttcacgagca attacaaatt
     2341 atagatgaaa tgtggcatac gtttattcaa tttacagatg cttataccag cttttgtgaa
     2401 aaatatcttg gtgcttacct ccaccattat ccaaatacaa atgacatgct aaaaaatgag
     2461 ataaggcatg ttaatgagca tggtataaca ttccaagaat atcgttttaa cgaatataaa
     2521 aatcaaattg aaaaaatcgc tttttaccta ggtcatgaaa ctgtcgcaaa gtggtatggt
     2581 gattatgctg taagatacag cataaagaac attaatacta taagaattcc aaaagaatcc
     2641 atatccagtg attcttacat cgaaaaagta aagagtatca ctcaccttcc agccgcagaa
     2701 tttgtaaaaa taataatgcg aaaagatgtt tggaatgata atggttctgt ttgtggttgc
     2761 agcggtaaag gatgtggcgc tgggtgctca tgtaattcta gataacataa agcccgtaat
     2821 atacgggctt taaggattat aaattgaatt ttattgaaaa gtacatcatc acatttaata
     2881 aatgtaattt gttttttatt atattattat caactataag tagttttctt ttcgttctat
     2941 tcggatattt aataaaaaca gttattgaca ataaagattc aataggagaa ataaactcat
     3001 tattaatatt tatagcatgt ttcttatcaa ttagattcct tatgcctgct ggatatagca
     3061 tatcggaata cttgactcaa aaaacaaata tagaattatc tgtaaagctt agagagcaag
     3121 ttattgataa tatacaaaat tctcaccaag agcatttttt aaagaaaaat aaaggagaac
     3181 taaataaggt tatagaaagc atgctttctt ctgcatcatc attattttat acaatatgtt
     3241 ctgatgtagt accattatta atacaaatga ttggaataat cataacaatt tgtataagtg
     3301 ttaatacatt aatagccatt gagtttataa taataatggc aatctatata atatttgtca
     3361 taaaaatgac acaacgtaga tttccaatga tgaaatcagt tgcacttagc tccaaattcg
     3421 catctggaag aatgtttgat atgatgcaca tgtatcctat ggataaagct ttccatacca
     3481 cggataaaag tcgtaaacga gtaattcaag ctgtaaatac acattcagaa aaacaaagaa
     3541 aagtaaataa tgaatttttc ctgtttggta tcagctccgc ttttctttca gtcattttta
     3601 gtagtctgat tatcctatca gcatactgga tgtttcttca tggtagagca agccgtggta
     3661 gtattattat gcttgccact tttttattcc aagtttttct cccattaaat cgcattggtt
     3721 atctatttag acaaataaaa atggcaagaa cagaaattga tttatactgt gccgaaataa
     3781 acgacataaa aaaaacaaat tacacagaca agcaatatct tcatgtaaaa gacaatattt
     3841 gcaatattga aatatacaac aaatcattca accataaatt aatattaaca aaaggcgttg
     3901 ttacctttat aaccggtgag aatggttcgg gaaaaacaac tattgcaaaa attctctctg
     3961 gcaatataac aacagaagaa aacacaataa aaatcaatgg gatacaacaa gaaaaagcaa
     4021 attctccttt tgttaatgtc ttatacgttc ctcaagatct agatctcatg cctggaacta
     4081 tagaggaaaa tatattacat tattccggca ttaataattt aaatacaatt aaaaagctgc
     4141 tacacagatt taagttcgac aaaccactag attatgagat caaaggttac ggacataatt
     4201 tatctggtgg acaaaaacaa aaattaggag tatctcttac atctggaaaa aacgtagact
     4261 tgattatttt cgatgaaccg acaaaagggt ttgattcact tggtataaaa ataatcagtg
     4321 attatataaa agaggaaaac aaggaaaagc atatcattgt tatatctcat gatgaacaac
     4381 ttataaataa tatacctaaa gcacagatta tagatatcac aaaacatgag gtttaaaaat
     4441 gaataatctt ataaaaaagg aaatcataga aaaatttaag aaatataatt tccagaaata
     4501 tccgtttgta ttcacggatg ttaattataa aaacttaatc aactggaatg atcttaataa
     4561 attgcttgaa aaagatatat tgcactatcc tagagttaga atggcaaatg acaactttcc
     4621 cgaaattagg gggtataaag gatttataag atacacatat agccaaacag gcgatagaac
     4681 accacatata aatcgccatc agctatataa atgcttacgt gatggcgcaa cccttatagt
     4741 agatcgatgc cagtcattct ttgaatctgt agatgaaagc agactatggt tatctaaaga
     4801 gttagaatgt acatgtagtg ctaatctata tgctgcattt acagcaacac caagctttgg
     4861 gcttcatttt gacaatcatg atgtaatagc tgttcaaatt gagggaataa aaaaatggaa
     4921 agtttacaac cctacctatt cataccctct cgaagatgaa agaagcttcg attatctacc
     4981 acctaatacc tccccagatt atgagtttga tataacccca ggtcaggcca tatatcttcc
     5041 tgctggatac tggcacaatg ttacgactca gagcaaacac tctcttcata tatcttttac
     5101 agttataaga cctcgtcgat tagaactatt taaaacatta tttgatgaat taaaaaacaa
     5161 tccatatatg cgggaaccta tagagcatgg tgattcatta tctgataaag aaaaaataaa
     5221 aactataata actaatgcta taaatagttt tgatattcag tttgcagaaa acatgcttaa
     5281 agctcaaagc agaacttatc gctataaaaa aataaaactt gaacatatat aaccattaat
     5341 ctaagggggc ttccccttag attttttcat acgatataaa ctgcattttc catccaccat
     5401 taacctttat ccaattctca ataaaagcca ttaatatatt tccatcattt tgctttattt
     5461 cagctttccc tgaaacacaa gcaatgcctt taaactcacg cattacgaca tcatattcat
     5521 atctttctat gctaggtaaa atcctcgctg gacgcatatc aatctttcgt aactgatgtt
     5581 ttttatagat aactttttga ccatttgttg aaataaacca atcagcttcc aagtaatcta
     5641 gcatttcagt attacctgaa taaaatgcat tataccattc ttttcttatt agatttaagt
     5701 ctgatttaaa attagtcaca ctaccttcct catatctaat tcctcctcta atctatcccc
     5761 aacattcctt actgatttta tagcatcatc aatagccaca ttatatatga ctgggattac
     5821 tcttttaaga atctcatcaa gtaattcctc tgattcaaac tgtccaaggc atatatcatg
     5881 ttcatctctg attttttgtg aaataaattc agctagtcct ttatatatgt ttttttcaat
     5941 atcagaaatt ttcattcttt atgaagcctc acaaataaat taataatata acatttttcc
     6001 tatcatagga aaattacaac tcaaccatac tgcaacctgg aatttcccaa gcaagcatat
     6061 ttaaataagt gcaggatacc acctcaaatc attctctaag gaacaagata atttaccata
     6121 atcccttatt gtacgcaccg ctgaaacgcg ttcggcgcga tcacggcagc agacaggtaa
     6181 aaatggcaac aaaccacccg aaaaactgcc gcgatcgcac ccggtaaatt ttaaccacat
     6241 gcatagctat gcagccatgt gaatcacgca ggaattgcca gccagagaca gctgaaacgg
     6301 atttttttca tgctttcgga agcgaagaag acggggaccg gagcgggaaa acagaagttc
     6361 aggggaatga gcgcgatctg gcaatagagg caaaacacag caacaaaaga cacaccagaa
     6421 tcgcgcccgt atgcgtttta acgcgttcct gtgttttcag caaggtctga ctgaagcgcg
     6481 ttcccggtgc ccccgaacca accattatcc caaaaaatgc accggaaacg cgctgacaac
     6541 attttagcct gtctctgatt accatcccag cgaagggcca tcctgtgtcc gttcctgtat
     6601 ttccagctgg cgctcacgct ccagggataa ctcatgttcg cgttcctgta tcagccgttc
     6661 atcccttatc tcctgttctc gctgtataac tggctcaagc gttctgtctg ctcgctcaag
     6721 tgc
//
LOCUS       DAZSIO010000183         6593 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZSIO010000183 DAZSIO010000000
VERSION     DAZSIO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.186"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..1076
                     /locus_tag="ACHX5T_003542"
     CDS             <1..1076
                     /locus_tag="ACHX5T_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAPES1 domain-containing protein"
                     /protein_id="HFR8468575.1"
                     /translation="AEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTA
                     STDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPV
                     NVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVA
                     GQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYV
                     SYVHDLPGGIRVSLSIDILYFITSSRKSVLFWILTALILLNMVRMHFRLYQNVSRENI
                     SDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAIT
                     LHDAYKASDERLYVNKQNKNSRS"
     gene            1119..1622
                     /gene="yeaK"
                     /locus_tag="ACHX5T_003543"
     CDS             1119..1622
                     /gene="yeaK"
                     /locus_tag="ACHX5T_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFR8468576.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1623..1727)
                     /gene="yoaI"
                     /locus_tag="ACHX5T_003544"
     CDS             complement(1623..1727)
                     /gene="yoaI"
                     /locus_tag="ACHX5T_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFR8468577.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1897..2343
                     /locus_tag="ACHX5T_003545"
     CDS             1897..2343
                     /locus_tag="ACHX5T_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFR8468578.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2300..3118)
                     /gene="nimR"
                     /locus_tag="ACHX5T_003546"
                     /pseudo
     CDS             complement(2300..3118)
                     /gene="nimR"
                     /locus_tag="ACHX5T_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            3215..4395
                     /gene="nimT"
                     /locus_tag="ACHX5T_003547"
                     /pseudo
     CDS             3215..4395
                     /gene="nimT"
                     /locus_tag="ACHX5T_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4450..4797
                     /locus_tag="ACHX5T_003548"
     CDS             4450..4797
                     /locus_tag="ACHX5T_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFR8468579.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4819..5073)
                     /gene="yoaF"
                     /locus_tag="ACHX5T_003549"
     CDS             complement(4819..5073)
                     /gene="yoaF"
                     /locus_tag="ACHX5T_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFR8468580.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5256..6281
                     /gene="dgcP"
                     /locus_tag="ACHX5T_003550"
     CDS             5256..6281
                     /gene="dgcP"
                     /locus_tag="ACHX5T_003550"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFR8468581.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6314..6412
                     /locus_tag="ACHX5T_003551"
     CDS             6314..6412
                     /locus_tag="ACHX5T_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFR8468582.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6415..6489
                     /gene="yoaJ"
                     /locus_tag="ACHX5T_003552"
     CDS             6415..6489
                     /gene="yoaJ"
                     /locus_tag="ACHX5T_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFR8468583.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
BASE COUNT         1596 a         1599 c         1615 g         1783 t
ORIGIN      
        1 ctgctgagca gtcggaagtg tcgccagaac agcggcgcgc catctgcgag gcttttgagt
       61 ctgccaataa tacccatggc ttaaacctga ctgcccataa atacccgggc ttacgcggca
      121 cactacaaac cgcatccact gactgcgaca caattgtgga agctgcagca ctattacccg
      181 cttttgatca ggcagtggaa ggcaaccgcc accaggatga ttacggttca ggtcttggga
      241 tggccgaaga gaaatttcac tattatctcg atctcaatga ccgctatgtc tatttttatg
      301 agccggttaa tgttgaatac tttgcgatga ataactggtc cttcctgcag tcaggaagta
      361 ttggcatcga tcgcaaagat attgaaaagg tatttaccgg gcgtaccgta ttgtcgagca
      421 tttaccagga tcagcgtact aaacagaacg tgatgagttt gctgacgccg gtatatgtcg
      481 cagggcagct aaaagggatt gtgctgctgg atattaacaa aaacaatttg cggaatatct
      541 tttacactca tgaccgccct ctcctctggc gttttctcaa tgtcacgcta accgataccg
      601 attcggggcg cgacattatc atcaaccaga gcgaagataa tctgttccag tatgtcagtt
      661 acgtccatga cttacctggc ggcattcgtg tctcgttatc cattgatatt ctttacttta
      721 tcacgtcttc gaggaaaagc gttctgttct ggattttgac ggcgttaatt ttgctgaata
      781 tggtccggat gcacttccgt ttataccaaa atgtgtcgcg agaaaatatt agtgatgcga
      841 tgactggact gtataatcgc aaaattttaa cccctgaact ggagcagcgg ttgcagaaac
      901 tggtgcaatc cggttcttcg gtgatgttta ttgcaattga catggacaag ttaaagcaaa
      961 taaatgacac cctcggtcat caggaggggg atttagcgat tacgttacat gatgcgtata
     1021 aagcttccga tgagcgttta tatgtcaata agcagaacaa aaacagccgt tcatgataac
     1081 cttctgtggt tgtttgcttg taatctcagg agcgtgaaat gactgaaatg gctaaaggaa
     1141 gcgtgacgca tcagcagtta atcgctttat tatcacaaga aggtgctaac ttccgcgtcg
     1201 ttacccatga agcggtaggt aaatgtgaag cggtgtcaga aattcgtggt accgctctag
     1261 gccagggtgc taaggcactg gtatgtaaag tcaaaggcaa tggcgtaaat caacatgttc
     1321 tggcgatcct cgcagccgat caacaggccg atctgagcca actcgccagc catataggcg
     1381 gattacgcgc ttcgttggcc agcccggcgg aagttgatga actcacaggc tgtgtcttcg
     1441 gcgcgatccc ccctttcagc ttccatccaa aactcaaact ggttgccgac cctctactct
     1501 tcgaacgatt tgatgaaatc gccttcaatg cagggatgct ggataaatcc gttattttga
     1561 aaaccgcgga ttatttgcgc attgcgcaac cagaactggt caatttccgc cgcactgcgt
     1621 agctagccta tccgttcaat aagcagaacg gacaaaacca gcacaaccgc aatagcaaaa
     1681 aacgatgatg tgataatcag tgtctcgacg aacatttgat cgttcatagc atgcccccgg
     1741 gagatagctg ttttcacgtt attgttagcg tgcacaaatg gcagtttgat gacagttcgc
     1801 ctgatttttt atgcaaaaaa cgtaaagatt ttctacttcc ttcctgcagc aagcataaag
     1861 taagcaggct tattattttt tggcaaggaa accacgatgt ttgatgtcac tttgctgatc
     1921 ctgctcgggt tagctgcgct gggctttatc agtcataaca ccactgtcgc cgtttcaatt
     1981 ctggtgttga tcattgtccg cgtcacaccg ttaagcacct ttttcccgtg gattgaaaaa
     2041 caggggctaa gtatcgggat aatcattctg acgattggcg ttatggcccc catcgccagc
     2101 gggacgctac cgccctcaac cttgattcac tcgtttctga actggaaatc gctggtggca
     2161 attgcagtag gcgttattgt ctcctggctg ggtgggcgcg gcgtgacgtt aatgggcagc
     2221 cagccgcaac tggttgctgg gctgctggtg ggcactgttt taggcgtagc gctgtttcgc
     2281 ggcgtaccgg ttggcccgct tattgccgcg ggtctggttt cgctgattgt ggggaaacag
     2341 tagttaatcc cgcaatatat cggcctggcg tttgccccaa gcctttctta aacatagtaa
     2401 taaaggccgt tgttgaatca taccccagcg tatgagcaac tttctgtaca gtatcgcctt
     2461 tcactaatcc ctgaagcgcc ataatcagct gtaactgctg acgccactgg cgaaaactca
     2521 accccgtctc tttcactatc aggcgggcaa gattgcgttc actcatcgca aaaaagccag
     2581 cccattgccc caatgccccc cactcgacgg gccctttcgc catcatctcc accatcgtgc
     2641 ggattttagg atgagaagaa acgggcaggt gcagctgttg ttgtggctgc tgagggagtt
     2701 catcaaacag gacctgaatt aatctgcggg tcatcggctc agcacgctga gtcgtcgtcc
     2761 tgtttgccag ggttaaaatc aattcccggc ataagggcga tattttcaat gtgcaacatg
     2821 gtcggcattg tcactgcgct gggttcgata aacagaaaac agagttcagc gttagctgtc
     2881 acttgattgc tatgcttcac cccgcccggt atccaaaccg catattgtgg cggcaccatc
     2941 cacaaagcat tttccaccgt acaggtaatt gcaccatgta atgccagtat cagctgtcct
     3001 ttgcggtgct gatgtacagg actggttaac tcatccgttc cggcgtgaat acaaaatgcg
     3061 accgcggctt catggtgtcg gtgggttcat agccattgag attcaacctg tgcatcattt
     3121 tgtccgaact tagcgataat ttgtcatttt agcttgattc aacataacaa taaaaacggt
     3181 aaggtacagc ctcgtttgta acaatgagaa gcatatgacc tgttcaactt cattaagcgg
     3241 caaaaacagg attgtcctta tcgctggcat tctgatgatt gccacaacat tacgcgtcac
     3301 ctttaccggc gcagcgccgt tactggatac gattcgttcc gcttactcgc tgacgacagc
     3361 gcaaaccggc ttattaacca ccctgccatt attggccttt gcgctaatct cacctctggc
     3421 tgccccggtg gcgcgacgtt ttggtatgga acgtagcctg tttgccgcgt tacttttgat
     3481 ctgtgcaggt atcgcaattc gttctctccc ttcgccttac ttattatttg gcggtacagc
     3541 ggtcattggc ggtgggattg cattaggcaa tgtcttactg ccaggattaa ttaaacgcga
     3601 tttccctcat tccgtcgcca gacttaccgg cgcatattcc ctgacaatgg gagctgcagc
     3661 ggcactggga tcggctatgg tcgtgccgct ggctttgaac ggttttggct ggcaaggcgc
     3721 gttgttcatg ctgatgtgtt ttcctctgct ggctcttttt ttatggctgc cacagtggcg
     3781 aagtcagcaa catgcaaatt tgagtacctc acgcgcctta catactcggg gtatctggcg
     3841 ttcgccactt gcctggcagg taacattgtt tcttgggatc aactcactgg tctattacgt
     3901 gattattggc tggcttccgg cgatcctcat cagtcacggc tatagcgaag cacaggcggg
     3961 ttcactgcat ggtttgctgc aactagccac agcagctccc ggtttgctga tcgcactttt
     4021 cttacatcat gtgaaagacc agcgtggtat tgcagcgttc gttgccttga tgtgcgcagt
     4081 gggcgcggtt gggctctgct ttatgccagc gcacgcgatc acctggactc tgcttttcgg
     4141 ttttggttcc ggcgcaacaa tgatactggg gttgacgttc attggtcttc ggcaagttct
     4201 gcgcatcagg cggcggcact ctcggggatg gcacaatccg tcgggtattt gctggcagcc
     4261 tgtgggccgc cgctaatggg taaaatacac gatgctaacg gtaactggtc cgtaccactg
     4321 atgggtgttg caatactttc actactgatg gcgattttcg gactttgcgc cgggagagac
     4381 aaagaaattc gctaatatcc ggtgctatag tgacgtgaca aatcttgcgt gaaagggaga
     4441 acaaacacga tgaatattca gtgcaaacgc gtttatgatc cggctgaaca gagcgatggt
     4501 tatcgcgtac tggtcgaccg cctctggccg cgcggtatca aaaaaaccga tttagccctt
     4561 gatgagtggg ataaagaaat cacgccgtca acggaactgc gcaaagcctt tcacggcgaa
     4621 gtcgtcgatt ttgcaacctt tcgcgagcaa tatcgcgcag aactggcgca acacgagcaa
     4681 gaaggaaagc ggctggcgga catcgccaaa aaacagccgc tgaccctgct ctacgcagca
     4741 aaaaacacca cgcagaacca tgcgctggtg ctggccgact ggctacgtag cttgtgattt
     4801 tagtacggca tccggcggtt atttttcacc agccggatgg tcccgtcgcc acaatgccca
     4861 ttcatcaatc gtttcaccgc ccggtaattt gcaattgttg ctgacccctt gcgctgtctg
     4921 cactggaatg agcgtcccgc ccttctgctg gcaatagacc gacgccggat ttgccatacc
     4981 aatctgcggc ggtttaggtg cttctggctg agaaggggtt gaacaaccag ccaggaccag
     5041 caagcaaggc agaacaaaac tgataatttt catttattga tctcacatat ttatccaagc
     5101 ttagagtatc gcggtatcgt tttgttttgc agcactattt ttattacatt cactcaaaac
     5161 atattacgtc ttgtttcatc tttgttgatg atgttttatc atgcctgcaa agattaaata
     5221 atcagcactt acccgccgta tcctggagtt gttccgtgtc agatcagatt atcgcccgcg
     5281 tctcgcaatc ccttgccaaa gaacagtcac tggaaagtct ggtccgacag cttctggaga
     5341 tgctggaaat ggtcactgat atggaatcaa cctacctgac caaagtggat gtcgaagcgc
     5401 gcctgcagca tattatgttt gcccgtaaca gccagaaaat gcacatcccg gagaatttta
     5461 ccgtcttgtg ggattactcg ttatgcaaac gcgccattga tgaaaactgc tttttcagcg
     5521 atgaagtccc tgaccgttgg ggcgactgta ttgcggcacg caatcttggc atcaccacat
     5581 ttctgagcac gccaattcac ttaccggatg gatcattcta tggcacgctt tgcgccgcca
     5641 gcagtgagaa gcgccagtgg agtgaacgcg cggaacaggt tttacagtta ttcgccggac
     5701 tgattgcaca atatattcaa aaagaggcac tggttgaaca gctgcacgaa gccaatgctg
     5761 cgctgattgc gcaatcgtat accgactcgt taaccgggct accgaatcgg cgggcgattt
     5821 ttgaaaatct gacgacgctg ttttccctcg cccggcatct taaccataag ataatgatcg
     5881 cgtttatcga tctggataac ttcaaattaa tcaatgatcg ttttggtcat aatagtggcg
     5941 atctgtttct cattcaggtt ggcgagcgcc ttaatacgct ccagcaaaat ggcgaagtta
     6001 ttggtcgtct cggtggtgat gagtttttgg ttgtttcact aaacaacgag aatgcggata
     6061 tttcgtcgct gcgagaacgt attcaacagc aaatacgtgg agaatatcac ttaggtgatg
     6121 ttgatttgta ttatcccggt gccagtcttg gcatagtaga agtcgatcct gaaacaaccg
     6181 atgcagacag tgccctgcat gctgccgata ttgcgatgta tcaggagaaa aaacacaaac
     6241 agaaaacacc ttttgtcgcg catccagcgc tacattcctg aggcgtattc acatcctttt
     6301 gattggtgat aacatgcgaa tcggtattat ttttccggtt gtaatcttca ttacagcggt
     6361 cgtattttta gcatggtttt ttattggctg ctatgctgcc ccgggagcat aaagatgaaa
     6421 aaaacaacga ttattatgat gggtgtggcg attattgtcg tactcggcac tgagctggga
     6481 tggtggtaac gtcacctcta aaaaatagca aaggctgcct gtgtgcagcc tttgtgcaat
     6541 ttaagcgtta acttttaatc ttcctgtaga taaatagcac gacaatcgca cca
//
LOCUS       DAZSIO010000184         6533 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZSIO010000184 DAZSIO010000000
VERSION     DAZSIO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.187"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..353)
                     /locus_tag="ACHX5T_003553"
     CDS             complement(<1..353)
                     /locus_tag="ACHX5T_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR8468584.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMA"
     gene            complement(350..934)
                     /locus_tag="ACHX5T_003554"
     CDS             complement(350..934)
                     /locus_tag="ACHX5T_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR8468585.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            complement(921..1298)
                     /locus_tag="ACHX5T_003555"
     CDS             complement(921..1298)
                     /locus_tag="ACHX5T_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR8468586.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            complement(1310..1696)
                     /gene="gpFI"
                     /locus_tag="ACHX5T_003556"
     CDS             complement(1310..1696)
                     /gene="gpFI"
                     /locus_tag="ACHX5T_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR8468587.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            complement(1748..2776)
                     /locus_tag="ACHX5T_003557"
     CDS             complement(1748..2776)
                     /locus_tag="ACHX5T_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR8468588.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            complement(2834..3181)
                     /locus_tag="ACHX5T_003558"
     CDS             complement(2834..3181)
                     /locus_tag="ACHX5T_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR8468589.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            complement(3218..4723)
                     /locus_tag="ACHX5T_003559"
     CDS             complement(3218..4723)
                     /locus_tag="ACHX5T_003559"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR8468590.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATDAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            complement(4713..6306)
                     /locus_tag="ACHX5T_003560"
                     /pseudo
     CDS             complement(4713..6306)
                     /locus_tag="ACHX5T_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(6303..6506)
                     /locus_tag="ACHX5T_003561"
     CDS             complement(6303..6506)
                     /locus_tag="ACHX5T_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFR8468591.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
BASE COUNT         1421 a         2069 c         1574 g         1469 t
ORIGIN      
        1 gccatctcat tatcacgttg atagttaaaa ccgagtggga tcatggtgtc gatgagtccg
       61 gaaagcaccg gaatatcctt catggccgga aatatggtgt tctccatcca cgtatccagc
      121 tcagaatccg gcgcctgtgc acggacgaaa acggcaatat gcagaacagc ctgccagtcg
      181 tcctcatccg tcatttttcc ggtgtactgc gcatcactca gccacaccgc cacggcgggc
      241 agttcctgcg catcaataaa tgccggaagc ccgtcaaaaa acgtggcgct gtctccacac
      301 tgttcccgaa ggcgtgccag cacgctctga cggatttgtg tgtgtcggtt catttactca
      361 gatgtaacct cagttgttgt ttcagggcat accccagctg tttcggcatt tccgcagcaa
      421 tgatgcggtc gcgggcatct tcaaatgcct gtgtcagcgg tccggacatc gggattttca
      481 ccacatcaat ggggtaacga tttttgccgt caatacgccg catcacatgc cagcgaccat
      541 tcgccagttg ctgaataaac gcatcccgga aaaggtattt ccccactttc agtacgctgc
      601 cacgatgacg tgagttgctg ccatgccggg agcgggccag tctgacctgt gcggtaccca
      661 gcttaatggc gggcaggtta ccccggttaa cacgcatgcg ggcatacatt tttcctgagg
      721 ggctggcttt aagcagcctg acacgctccc tgaccgtttt caggggaata cctttcacct
      781 ggttatcccc ggcaacggta ttctgcgcaa cctgccgtgt ggcgacagaa acaatctttg
      841 ccgccacacg gtttaccgcc catgcgctgg tctgtggcac catgcgggta tcgaggctgt
      901 tcagattgcg gatggcattc tcaagtccct tcatcatatc gcctcattct ggcattattc
      961 tgtggggcag gactgccccg ccttaaccgg atataacagc atcccccgtc atccggagta
     1021 atacgatcca caaagaaaga atcatccccg atagttaacg tatcctgacg gcgtaactgc
     1081 gatatatctg ccgttttcac aaacaggcac ggtgaggaat cctcaagccg gatccccccg
     1141 gcaacaaaag aaatactttc gggatcatca aaaacccctg tcaggtgtgt cccctcaagt
     1201 tctccggacg taatgacggc gctgatcccc atatcacgga gaatgacttc attgacaccg
     1261 gcaagcgcag catcaaacag attatcgaaa ccgtccacga catctcctgt tactgccttt
     1321 ttgccagacc acctgccgcc atgctggcag ccacctcagc agaaacacga aacgacgttc
     1381 ccggtgaaac aaatatcact gaaccattac attttgtatg cagtgtcgcc agtgccacca
     1441 ccgtaacctg ccccgtttcg tctccggtaa caatgcctgg ctgttcagga actgactcgc
     1501 ctttatgcgc ggtcggatca tctttcgatg gtgagtacgt atccggctct gcgacctccc
     1561 ccgcatctcc aagctcttcc tccagttccg ccactcgctg cgcaatatct gccgcactac
     1621 cggagacatc cggttcacgc cccagcctct cagccagctc acaaagacgt ttcaggtttt
     1681 cttctttcgt agccataccc gctcctgatt gaatgaaaaa cgggagcatt tagctcccgc
     1741 tcacggatta tttcacctgc accaccacaa actcatccgg atccggcagc accatcagcg
     1801 gcgcggactg cgtcatggtg aattcacgcg ccggatcacc cacggtcagc cagtgtttcg
     1861 gataacggga agacgccacc acgccttcag acaatgcctg cgcatcctga atggcaccgt
     1921 agcaacgaat accctctgcg gccgtatttc ccagtaccag tgtgccgtcc ggcagataac
     1981 gtttttcggt accgtcctct gccacatagg acgtttttgc caccacgatt gccagatcac
     2041 cataataccc tttgaaggac accaccgccc ccaggtcttt cactgccgtt tccagctgtg
     2101 aatttgagcc acgacgggta tccagttttt cgcggaacag cttaaagcca ttcagcagac
     2161 gccagacctt accatccata atggcaatat tgatggtgcc ggaagcaaaa tcacagtacg
     2221 catccagatc atgcgtcgga tcaaaggtat cgcggtcctg agcgctccac tccgtccctc
     2281 ctgcctgcgt aatgttattt cccaccgacc ggccaaaatc gacctcaaca gtttcaaact
     2341 gctctccttc catggtgtat ttgccataca gcacggcatt caccgcctgc atctcttcca
     2401 cctggacaat ggcgtgctct tcctgtttga ggttatcggt gatgatacgc aggcgacggt
     2461 aagccgggcc gttcagctgg gccggatctt caccgggaag acgctccacc gcctgctggt
     2521 aattaaattc gtgtttcggc ttaacgtagc ccgggcgcag tacacgagtt tcaccgccac
     2581 ggtgacgcaa cacctttcct tcaacaaccg gggagacata ggccgccacc ggcgtttttc
     2641 cggtaatttt gtccagcatt acctcttcgg tatggaaatt caccgtacgg cggaaaaaca
     2701 gctccagaaa cagcgcacga aatttaactt tttgttcggt ataaccgagt aactggcggg
     2761 tcgtaaacaa tcccataaat cagttccttt cattcagaaa tcagtcaggc taccgcagtg
     2821 acctgataaa atgttacggc agcgctgcgt gactcagggc actgccggca aaggcattcg
     2881 cctttttgtg tgtatccact ctctcaggcc agtggatggc ctccgtcgca aaggtccccg
     2941 acttgtaata cgtcagcacc gtctctgtac cttcaagcgg cagtaccagt atgccaacca
     3001 catggcctgc cttctgtccg tcccagacca ccagtttccc ggtatcctca tccagcatca
     3061 ggggcgtcag agccggtgtt gccgaggaaa tcccgctgca gcctgtggcg gtatgggccg
     3121 gatcattacc ggcaaaaata cgtacttccg cacgctgttc agtgatggtt ttcgttacca
     3181 tattgtaaaa acctcatatt gatggtcagc actgacttca tggcatggcc atgagcattt
     3241 tcacgtccgc atcaccgtct gctgacgtct gtggcacgcc accctgtacc gctgccggtg
     3301 aatggttcgc catgaaatgt tcaaacatgg cggttgtgga tgcagaaacc ggttctgccc
     3361 tgcctgaggc tgccgccagt acagcccggg cattctccac ggtcatcccc ggacaggccg
     3421 ccagtttttc agcctgcgcc tctgcccctt ttgcctcatc cagggccata atctgatcac
     3481 gaagtgaggg tccggcattc gcctgcggtg aagcagccag aatcggacgg gctttttcca
     3541 ccgtcatctc cggcatcgcc gccagcgttg ccgccagttg ttcacgtcct ttcgcctctt
     3601 cacatgccat aatgcgatcg gcttcactct gcgcagatac accagattgt tgcggtgccg
     3661 ccacggccag aatcgcccgg gcctgctcaa cgctcatacc ctgttgccct gccagcatcg
     3721 tggccagttg ttcacgtcct ttcgcttcct gacacgtcag gatccccatc actcgctggt
     3781 tctcctgcgc ggcagcatcc gttgcagtta attgcggcat agtgcctcct gtatcgtgtg
     3841 tgttcagcgt cgcagccatc acgctgatgg cgtccgacgc attgattaat tcatccgcca
     3901 gttcggcctc aataccggac tgaccttcaa aaacggcggc ctctgttccc ataaccgcat
     3961 ccacagataa accggtatac atcgccactt tttcagcaaa catccggcgc gccgcatcaa
     4021 tgcgctgctg catgtcctgg cgaacctttg ctggcaggga ttcaaactga ttgccatcca
     4081 ccttgtgcga tccggcgtaa atcagcgtga tatccacacc ggcctgtgcc agatgaccgg
     4141 catagctgac atggcccatc atcaccccaa tggagccgat acgggatgtc tgggtaacca
     4201 gccgtcggga gcaggccgat gccagtaaca tggcggctga acaggccgtg tcattgcaca
     4261 gtgcccagac aggcttcagc tgccggagac ggtaaatcat gtcagcgcag tcaaacgcgc
     4321 cggtggcctg cccgccagaa ctgtcaatgt ccagcagtac gccccgtacc tgagtgtccg
     4381 ccattgcctg ctgaagacag gcgacaatgc cgtcatagcc agtcattccg gaaaatggcc
     4441 gcatgccacc cagccggtgt accagcgtgc cggtcaccgg cagtacagca ataccgttca
     4501 ccacccggta aacacgggca ggtcgtttgc ctccggccat gtactcgtcc gtttcagcca
     4561 gcatcccggg agcgtcaagc tgtacctgct gttgcggtac cgaaaggctt gctgcgccca
     4621 tctcacgccc gagcgcgcaa aagaaaaccc gcgcataggc gggctccaga agcagcggct
     4681 cattgaatgc cgcagcgata atatgtgaaa gattacgtct cacgtgatgt tgtctcctct
     4741 tccggcctgc gactctccgc tatctgctgc tgatacgcct gggctatcca caccggacgt
     4801 gagagtccgg ctttttcgcg ctctgcggat tccctgacct gctggcggaa aatgtcctga
     4861 taatcttcgc ccatcagtgc cagttctttc tcatacgtgc tcagtccggc ctcaatgcgc
     4921 atcaccgatt cctggacctc cttgagcccg tcaatggcca ttcttccgga gctaatccac
     4981 tctgcccgtg accaggctga acgcgcctgg taaaaatcaa aacgcgcccg tggcggacga
     5041 ataatccccc gaagaagcgc ctcttccagc cagcaggaaa acatctgcgt ggccagccgg
     5101 gccgcaataa attttcgccg ccccataaaa tagcgccacg actcattggc ggaggcgcgg
     5161 gcacttgagt aactgacctt cgagtaatca cgggacaact gttcgtagga aacgccaaga
     5221 ccggctgcaa tataccgcag cagcgcctgt tcaagcgccg aaaatccatt gtctgaatcc
     5281 tgcgctgtct gcaggttcag cgcatcgcca ggaaaaaagg tgcggaattt taacaccgcc
     5341 cagcgtcacg ttattcgtgt cataccagcg ggtgaacttc tccagaatat taataagcgg
     5401 attatcctgc tgaccttgcg gtgcccccgc gatatattca aatgcctttt cggtatcaag
     5461 ttcactttcg attgtggctg catacatcgc cttcacaatg gccgactgaa gctgcgtggc
     5521 ctgcagggag tccagcatct tcatccgctc cattacgctg taaaactgat tagccccacg
     5581 ggtctgcccg tcctccaccg gctcaaaaat atgcagcatg gccggacgcc cggtgggaag
     5641 ctcacgcggg atccgctccc agcgtccggc accagagaac ggaaaatcat cctcacagat
     5701 atggtacgca atcgcccgcc cataccgatc gacctcaacc ccggcccgca gaaagtggtt
     5761 ccccataccg tgtcctggcg tgtccacccg tttcggactc acggctttaa aacgcgtacg
     5821 gaataactgc gtggtttccg tatcccagac cggctgcaca aagatttcgc cgttaaaggc
     5881 atgcacgccc accccttcac ggataaattc cgtaaacgtg cgtttccctt ccacgtcgat
     5941 ctcgccaaac attccttctg cgtattccga ccaggctgct tccacctccc cgacaaaact
     6001 ttttgccgtg gtctcccgca tccccagcca acgccagttc agacggtagc tgataagaaa
     6061 catgtgcccg acgatatgat ccttatgcag ggccaccgca ttagccgcga taccgttatt
     6121 gcgcaccaga tcatctgccc gggcattccc cagacgcaat gcaggcaaca gggccgcatc
     6181 ggcactctgc gccggtggta accactctgc catttgcccg ccaaatcctg cgccgcctcc
     6241 gttgtagctg agactctcac gaagcggaac gccgttcaca tcaatcagga caggcgttcg
     6301 tttcataacc tcactcccag cggtcggcgg cgtcgggtcg tacccagaac cgactccgca
     6361 tcgttgatcg cacggttaag ctcattcagg gacgctgccg tatattcgat cctgcgaccg
     6421 tctttctgta ccgacacaac ccgtttaccg gttaataaat caaggcgcgc ctgacgcagc
     6481 gcctgcagtt cagcgactgt aaccattcac tcctccggac agcttcgctg cca
//
LOCUS       DAZSIO010000185         6520 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZSIO010000185 DAZSIO010000000
VERSION     DAZSIO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.188"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..115
                     /locus_tag="ACHX5T_003562"
     CDS             <1..115
                     /locus_tag="ACHX5T_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468592.1"
                     /translation="LDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            439..1827
                     /gene="narU"
                     /locus_tag="ACHX5T_003563"
     CDS             439..1827
                     /gene="narU"
                     /locus_tag="ACHX5T_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR8468593.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1909..5649
                     /gene="narZ"
                     /locus_tag="ACHX5T_003564"
                     /pseudo
     CDS             1909..5649
                     /gene="narZ"
                     /locus_tag="ACHX5T_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5646..>6520
                     /gene="narH"
                     /locus_tag="ACHX5T_003565"
     CDS             5646..>6520
                     /gene="narH"
                     /locus_tag="ACHX5T_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR8468594.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1558 a         1713 c         1690 g         1559 t
ORIGIN      
        1 cttagatgaa ataaacaata cttactataa taacgattat tacaatcagg caataagaga
       61 ggaaccgata gactttctgg acagatcgtt ttcctccaat tcatggcctt tttagtgaat
      121 aaatcatcac taattctata agtgtctggt cttgttgaga atatagttaa tatgtaaccc
      181 tttagattca cgatctgaag ggttgaaaca tgacatacgt tcaaagatta acccccgttt
      241 gaaggttagc ttgttagtta catttagtaa cacatatctg gctacttaat ccccattctg
      301 cccccgcggt tacgctttct tatctattct ttcaatcagc acttcaccca attcatcctt
      361 aattctaata actggctatt gcgccttacc tcctgttttg tcttttttca ttaccaatgt
      421 gtgcatatga ggaacaatat ggcactgcaa aatgagaaaa atagtcgtta tcttttacgc
      481 gactggaaac cagaaaatcc ggccttctgg gaaaataaag gaaaacatat tgctcgaaga
      541 aacctctgga tatcagtcag ttgtctactt cttgccttct gtgtctggat gctatttagc
      601 gcagttaccg ttaatctcaa taaaatcggt tttaatttta ctaccgatca actcttttta
      661 ttaaccgcat taccctccgt ttctggcgca ttattgcgtg ttccctactc ctttatggtg
      721 cctatattcg gtggacgccg atggccggtt tttagtactg caatcctgat tattccttgc
      781 gtctggctcg gaattgccgt gcaaaatccg aatactcctt ttgggatatt tatcgttatc
      841 gctttgctat gcggttttgc aggtgcaaac tttgcttcga gcatgggcaa tatcagtttc
      901 ttctttccaa aagccaaaca agggagcgct cttgggatta atggcggatt aggaaactta
      961 ggtgtaagtg taatgcagct ggttgcaccg ctggtcattt ttgtacctgt atttgccttt
     1021 ctcggcgtca atggcgtacc gcaggccgac ggttcggtga tgtcgctggc gaatgccgca
     1081 tggatttggg taccgctact ggcgattgcc acgatcgccg cctggtcagg gatgaatgat
     1141 atcgccagtt cacgcgcctc aattgccgac cagctccctg tcttacaacg cctgcatctc
     1201 tggctgctga gcctgcttta ccttgccacc ttcggttcgt ttatcggttt ttctgcgggt
     1261 tttgccatgc tggcaaaaac ccagttcccg gatgtgaata ttctgcgcct ggcgttcttt
     1321 ggcccattta ttggtgccat cgcgcggtcg gttggtggtg ctatttccga taaattcggc
     1381 ggcgtgcggg tgacgttgat caactttatt tttatggcga ttttcagtgc cctgctgttc
     1441 cttaccttac cgggcacagg ctccggtaat ttcatcgcct tttacgccgt atttatgggg
     1501 ctgtttctga ccgcgggtct gggaagtggt tctactttcc agatgatcgc cgtcatcttt
     1561 cgccagataa ccatttatcg ggtgaagatg aaaggcggta gtgatgagca agctcagaga
     1621 gaagccgtca ccgaaacggc ggcaacgctg ggctttatct cagccattgg cgcagtgggc
     1681 ggctttttta ttccgcaggc atttggcatg tcgctcaata tgaccggctc tccggtcggc
     1741 gcgatgaaag tgtttttaat cttctacatc gtttgtgtgc tgctgacctg gctggtttat
     1801 ggtcggcgga agttcagcca aaaataagag atcgatgcct gatgtacaac catgtcgcat
     1861 ccgacaaggc atcagatccc aattcattcc tggagcagga gtcatgtcat gagtaaactt
     1921 ttggatcgct ttcgctactt caaacaaaag ggcgaaacct ttgccgatgg tcacggacag
     1981 gtgatgcata gcaaccgcga ctgggaggac agctatcgcc agcgttggca gttcgacaaa
     2041 atcgtgcgtt ccacccacgg cgttaactgt acaggctcct gtagctggaa aatctacgtt
     2101 aaaaatggtc tggtgacctg ggaaatccaa cagaccgact acccgcgcac tcgccctgac
     2161 ctgcccaatc atgaacctcg cggctgcccg cgtggcgcaa gttactcctg gtatctttac
     2221 agcgctaacc gcctgaaata cccgctcatt cgtaaacgac tgattgaact gtggcgcgaa
     2281 gccctcaaac aacacagcga tccggtactg gcgtgggcat cgattatgaa tgatccgcaa
     2341 aagtgcctga gctacaaaca agtgcgtggg cgcggcgggt ttatccgctc caactggcag
     2401 gaactaaacc agctgattgc cgccgctaac gtctggacca tcaaaaccta cggcccggat
     2461 cgcgttgccg gtttctcgcc gatcccggcg atgtcgatgg tttcttacgc cgccggaacg
     2521 cgttatctgt cgctgcttgg cggcacctgt taaagtttct acgactggta ttgcgacctg
     2581 ccgcccgcct cgccgatgac ctggggcgag caaaccgacg taccggaatc tgccgactgg
     2641 tataactcca gctacatcat cgcttggggg tctaacgtac cgcagacacg tacgccggac
     2701 gcccacttct ttaccgaagt acgctacaaa ggcactaaaa ccatcgccat tacccctgac
     2761 tactctgaag tggccaaatt gtgcgaccag tggctggcac cgaaacaagg cactgatagc
     2821 gccctggcga tggcaatggg gcatgtgatt ttaaaagagt ttcatctcga taatcccagc
     2881 gactacttta tcaactactg ccgccgctac agcgacatgc cgatgctggt aatgctggag
     2941 cctcgcgacg atggtagcta cgttcccggg cgcatgatcc gcgcatctga cctggtggat
     3001 ggactgggcg aaagcaacaa tccgcagtgg aaaaccgtag cagttaatac cgcaggtgaa
     3061 ttggtagtgc cgaacggttc gattggtttc cgctggggag aaaaaggcaa atggaatctg
     3121 gaatccattg ccgccggtac ggaaaccgaa ttgtcgttaa ccctgctcgg tcaacatgac
     3181 gctgttgcag gcgtggcctt cccctacttt ggcggcattg aaaatccgca ttttcgcagc
     3241 gtaaaacaca atccggtgct ggtgcgccaa ttgcccgtta aaaacctgac gttagtcgat
     3301 ggcaacacct gtccggtggt cagcgtttat gatttggtac tggcgaatta cggcctcgat
     3361 cgcgggctgg aagatgaaaa cagcgcgaaa gattacgctg aaatcaaacc gtacacccca
     3421 gcctggggtg agcaaattac cggcgtaccg cgccagtata ttgaaaccat cgcccgcgaa
     3481 tttgccgata ccgcccataa aacgcatggg cgctcaatga ttatcctcgg cgcaggtgtt
     3541 aaccactggt atcacatgga catgaactac cgcgggatga tcaatatgct gatcttctgc
     3601 ggttgtgtcg ggcaaagcgg tggcggctgg gcacactatg tcggccagga aaaactgcgc
     3661 ccacaaaccg gctggttgcc actggccttt gcgctcgact ggaaccgacc accgcgccag
     3721 atgaacagca cctcgttttt ctacaatcat tccagccaat ggcgctatga aaaagtctct
     3781 gctcaggagt tactttcacc gctcgccgat gccagtaagt acagcggtca tctgattgat
     3841 ttcaacgttc gcgccgaacg tatgggctgg ctaccttctg cgccgcagct ggggcgtaac
     3901 ccgctcggga ttaaagctga agccgacaag gccggattat cccccacaga atttaccgcc
     3961 caggcgctga aatcgggcga tttacgtatg gcctgcgaac aaccagatag cggcagcaat
     4021 catccgcgta atttgtttgt ctggcgctct aacctgcttg actcctccgg caaaggccac
     4081 gagtatatac agaagtatct gctggggacc gaaagcggga ttcagggcga ggaactcggt
     4141 gccagcgacg ggatcaaacc ggaagaagtc gagtggcaaa ctgcggcgat tgaaggcaag
     4201 ctcgacctgc tggtgacgct cgacttccgc atgtccagta cctgcctgtt ctccgatatc
     4261 gttctgccca ccgccacctg gtatgaaaaa gacgatatga acacctcgga tatgcatcca
     4321 tttattcatc cgctttctgc ggcggtcgat ccggcctggg aatcacgcag cgactgggaa
     4381 atctacaaag gtattgccaa agcattttcg caagtgtgcg tgggccatct tggcaaagaa
     4441 accgacgtgg tattacaacc actgctgcat gactctccgg cagagctctc acagccgtgt
     4501 gaagtgctcg actggcgcaa aggcgaatgc gatctgatcc cgggtaaaac cgcgccgaat
     4561 attgtggcgg tggagcgcga ctaccctgct acgtatgaac gctttacctc gctcgggcca
     4621 ttgatggaca aacttggcaa tggcggtaaa gggatttcgt ggaataccca ggatgaaatc
     4681 gatttcctcg gcaaactcaa ttacaccaag cgtgatggcc cggcacaggg gcgtccgctg
     4741 attgacaccg ccattgacgc ttcagaagtg attctggcac tggcaccgga aaccaacggt
     4801 catgttgcag tcaaagcgtg gcaggcgctg ggcgagatca ccgggcgcga acatacccat
     4861 ctggcgctgc acaaagagga cgagaagatt cgctttcgcg atattcaggc gcagccgcgt
     4921 aaaattatct ccagccccac atggtccggt ctggaaagcg atcacgtatc ctataacgcg
     4981 ggatacacca acgttcatga gttaattccg tggcgcacgc tgtcgggacg ccagcagctc
     5041 tatcaggatc atccgtggat gcgtgctttt ggtgaaagcc tggtggctta tcgcccgcct
     5101 atcgacaccc gtagcgtcag tgagatgcgc cagataccgc caaacggctt ccaggaaaaa
     5161 gcacttaact tcctgacgcc gcaccagaaa tggggcattc actcaaccta cagtgaaaac
     5221 ctgctaatgc tgacgctctc tcgcggtgga ccgattgtct ggatcagcga aacagatgcc
     5281 cgagaactga ccattgtcga taacgactgg gtggaagtat ttaacgccaa cggcgcgctg
     5341 actgcccgcg cggtggtcag ccaacgtgta ccgccgggca tgaccatgat gtatcacgcc
     5401 caggaacgca ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg cggcattcat
     5461 aactcggtca cccgcgtttg cccgaaaccg acgcatatga ttggcggtta cgcgcagctg
     5521 gcctggggct ttaactacta cggcaccgtc ggttcgaacc gcgacgaatt catcatgatc
     5581 cgcaagatga agaacgttaa ctggctggat gatgaaggtc gcgatcaggt acaggaggcg
     5641 aaaaaatgaa aatacgttcg caagtcggca tggtgcttaa cctcgataaa tgtatcggct
     5701 gccatacctg ttcggtgacc tgtaacaacg tctggaccgg acgcgaaggc atggagtacg
     5761 catggtttaa caacgtcgaa accaaaccgg gcattggtta tccgaaaaac tgggaagatc
     5821 aggaagagtg gcaaggcggc tgggtccgcg atgtgaatgg caagatccgt ccgcgtctgg
     5881 gcagcaagat gggcgtaata accaaaatct tcgccaaccc ggtggtgccg cagcagattg
     5941 atgattacta cgaacctttc accttcgact acgaacattt gcatagcaca ccggaaggca
     6001 aacatatccc tactgctcgc ccgcgttcac tgattgacgg caaacggatg gacaaagtga
     6061 tctggggccc aaactgggaa gaactgctgg gcggcgagtt tgaaaaacgt gcccgcgacc
     6121 gcaacttcga ggccatgcaa aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt
     6181 acctgccgcg cctgtgcgaa cactgcctca atcccagctg cgtggcgacc tgcccaagcg
     6241 gcgctatcta caaacgcgaa gaagacggca ttgtgctgat tgatcaggat aaatgccgtg
     6301 gctggcgttt gtgcattagc ggttgtccgt acaaaaaaat ctacttcaac tggaaaagcg
     6361 gcaagtcaga aaaatgtatc ttctgttatc cgcgaattga gtcaggccaa cccaccgtgt
     6421 gctcagaaac ctgcgtgggt cgcatccggt atctgggcgt gctgctttac gacgccgacc
     6481 gcatcgagga agcgtcgagc accgagcgcg aagttgacct
//
LOCUS       DAZSIO010000186         6374 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZSIO010000186 DAZSIO010000000
VERSION     DAZSIO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.189"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..378
                     /locus_tag="ACHX5T_003566"
     CDS             <1..378
                     /locus_tag="ACHX5T_003566"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468595.1"
                     /translation="IIRWLLKKRTNGSIRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACHX5T_003567"
     CDS             complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACHX5T_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR8468596.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACHX5T_003568"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACHX5T_003568"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR8468597.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="ACHX5T_003569"
     CDS             complement(1787..2476)
                     /locus_tag="ACHX5T_003569"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR8468598.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACHX5T_003570"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACHX5T_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR8468599.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACHX5T_003571"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACHX5T_003571"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR8468600.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACHX5T_003572"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACHX5T_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR8468601.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1621 a         1716 c         1577 g         1460 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagta tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcagaatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
     6241 atataacatc attgttttca atctgccatt tatgggattg accgttttct tttgacacgg
     6301 agttcaacaa tgggtaatgc ttccaactta ctgatttagt gtatgatggt gattttaagg
     6361 tgcttgcgtg gctt
//
LOCUS       DAZSIO010000187         6317 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZSIO010000187 DAZSIO010000000
VERSION     DAZSIO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.190"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(99..749)
                     /gene="zinT"
                     /locus_tag="ACHX5T_003573"
     CDS             complement(99..749)
                     /gene="zinT"
                     /locus_tag="ACHX5T_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFR8468602.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(1007..1642)
                     /gene="msrQ"
                     /locus_tag="ACHX5T_003574"
     CDS             complement(1007..1642)
                     /gene="msrQ"
                     /locus_tag="ACHX5T_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR8468603.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            complement(1643..2647)
                     /gene="msrP"
                     /locus_tag="ACHX5T_003575"
                     /pseudo
     CDS             complement(1643..2647)
                     /gene="msrP"
                     /locus_tag="ACHX5T_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            complement(2756..3169)
                     /gene="hiuH"
                     /locus_tag="ACHX5T_003576"
     CDS             complement(2756..3169)
                     /gene="hiuH"
                     /locus_tag="ACHX5T_003576"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR8468604.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3302..3973
                     /gene="hprR"
                     /locus_tag="ACHX5T_003577"
     CDS             3302..3973
                     /gene="hprR"
                     /locus_tag="ACHX5T_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR8468605.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3973..5331
                     /gene="hprS"
                     /locus_tag="ACHX5T_003578"
     CDS             3973..5331
                     /gene="hprS"
                     /locus_tag="ACHX5T_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR8468606.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(5439..6290)
                     /gene="hchA"
                     /locus_tag="ACHX5T_003579"
     CDS             complement(5439..6290)
                     /gene="hchA"
                     /locus_tag="ACHX5T_003579"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR8468607.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
BASE COUNT         1769 a         1332 c         1456 g         1760 t
ORIGIN      
        1 gagcgtgtct gagtttgtct tacttatttg tcttggtaaa ttattgttgt tactaccagg
       61 cactgttctg ttaaataact gaagtcagcg caaaaaaatc aatgagacat catctcctcg
      121 accacttctt cgctactcaa ctgataggga taatacgttg gccagttttc catttcattc
      181 aataaagatt gctgggaatc attacccata aaaatgtgaa aatgagatga ttttcgtggt
      241 gcaattatat gatcgctaaa ttgtatatat ttaggggctt tgctttcagg atctttacat
      301 tcgaataagt aacgaacgcc tttcttgcct gatttatagg tgagtatttt gtatccatcg
      361 taatcatatt tacaggatgt tgtttcatta tttctatgga attcgacaat gccgtcctca
      421 atgccaatca tttcgatatc tgtcggataa cctttgcgat aataatcttt aatttcagca
      481 aatgttttgg ttttatctgc atccgctttc ttctgaaaga cgggatcaag tttgccactt
      541 tgcagtaaag ggtaaacgga ttgccagact ccatcccagt cactgagcgt tcggttttgt
      601 acattggtat catcaaaaac accattagca gctttttgtt cgacctctgt taagggtttg
      661 ccatgagaat gatgaccatg cgaaaaggca ggagcgctaa caataaagac acctaaagca
      721 acagccagtt tatgaagacg aatcgccaaa acaatttcct ccagagttaa tgtacattat
      781 gtgtaatgtt atattgtaac atatgaaaaa atgcaacaac tcattaggaa atgagagcat
      841 ggtattctca ggtgtattga actatcttat tgaaataaaa taaattattc tggtgatgaa
      901 aggttgatga caaacgaaac ataacttgat gcgatctgtg tgggccggat agggcgctcg
      961 cgccgcatcc ggccgggata acgcgaagtt tgtcggcaat atgaagttat agagataatt
     1021 tgttgtgggc ctgcttgcgt aaccggttga aaagggaaag caacttctta taccgtaagg
     1081 ctaaaagcag tacagccagc ccggcgtaga tgagtggctg cggtgaaata atcttcactg
     1141 accacagata atgtattggg gccaggatcg cgacaagata gacgaagtta tgcaactgtt
     1201 gccagtgttt gccgagtttt cgctgcatcg actgggttga agtgaacgct aaagcaagca
     1261 aaataaccca gctgataatt cctaacgtta aataaggccg ggtaattaac tccttaccta
     1321 ataacgccag attattcacg cccaactcca gcaatgcgta actggttaaa tgcagtgtcg
     1381 cccaggcaaa gcaccataag cctaacaggc ggcgagtgcg tatcaataac ggctgttttg
     1441 cgtagcgtgc cagaggtgtg attaacaagg ccgcaagcaa aaatttcagt gcagtgcgac
     1501 cggtaaaatg ctgaatatct ttcaccggat cggcacccag tccaccgtga ttgatcgccc
     1561 agaccagcca gagaaacggc aacaatccgg caagatgcag gcaaactttc agccatatca
     1621 cttgttttgc tgtcagacgc atttagaaat tctcccgcaa atccaggcca cgatacagcg
     1681 atgccacctg gtcggcgtaa ccattaaaca gtagtgttgg ctggcgctga acatcgagaa
     1741 tgccgcctga accaataaat cgttcggtag cctgtgacca gcgcgggtga tcaacatgcg
     1801 gattaacgtt ggcgtaaaaa ccgtattcgt caggcgctgc cagattccag gtggttggcg
     1861 gacgctcgcg ggtcagctta atactgacga tcgatttaat ccctttaaag ccatatttcc
     1921 acggcacaat cagtcgcacc ggcgcgccat tttgcggtgg taacgccttg ccataaacac
     1981 ctacggtcat cagtgtgagc ggatgcattg cttcgtcgag acgcaatcct tcgacataag
     2041 gatatttcag cccgccgccg ataaagcggt cctgctggcc aggcatctgt tccggcgcat
     2101 aaatcgtttc gaaagcgaca tacttcgcat tgctggtggg ttcggcaagc gccagcaatt
     2161 tgtgtagcgg aaaaccaatc cacggcacca ccatcgacca cgcttccacg cagcgcatac
     2221 gataaatacg ctcttccagc gggaagcgac gggttaaatc atcatgatcg agggtcaatg
     2281 gttttgccac ttcgccgctg attttcagtg tccatggatc ggttttcagg ctaccagcat
     2341 tagccgcggg atcgtcttta tccagcccga attcatagaa gttgttataa ccggagactt
     2401 tatctgctgg cgtcagtggc aggttatttt gctaggcggc aggcttgctg aactccagcg
     2461 gttttccggc gggggcgggc ggacgatcgt tccctttaaa ccagctaagc agatcggcat
     2521 gcgcagcgtg aggcaaagaa aatgcagctg cgctgatgcc cagtgctttt aacacctgcc
     2581 gacgcttcat aaagaatacc gactcggccg taacatctga ttcttttaaa aattgcttct
     2641 ttttcataag ccatcacact tttttgtcgt taaccaacaa gagctgttta ctgttaatct
     2701 gtcagggagt cacctcccta acagtagcag agatcagctt ttacatattt acgcattaac
     2761 tgccacgata ggttgaatac ccatattggc tgagtaacaa aggcacatga taatgctcgt
     2821 tcactttatt aatatgaaat tcaacgggaa tctcagggaa gaaactttca agattttgtt
     2881 tcttgaaata gtccccggtt ttaaatacga cacggtaatc gccagtagtc gcagtttgct
     2941 cgggccacag tgccttaatt cgtccatcct tatctgtttt ggcggtatta agttgtaacc
     3001 agccgttatc cgctttcttt tcaagagtga ctgtcacgtc ggcagcgggt tttcctgttt
     3061 gctggttcaa aatgtgcacg ctaagaatgt tttgttgtgc ggcataaacc aaagaaggta
     3121 atgaaaaggc tgccgttgct accgagagta ctaaataacg ctttaacatg tttatatcct
     3181 tgtcatgtga atgagttttg aggatagcga aaaaacgtcc agggtaacct tacagcagca
     3241 ttacaatttt gtaatgaacc aggttgtttc tataacatat gatttatggc atattatttt
     3301 catgaagatt ctacttattg aagataatca aaggacccag gaatgggtaa cgcaggggct
     3361 ttccgaagcg ggttatgtca ttgatgccgt ttctgatggc agagatgggc tttatcttgc
     3421 gctgaaggat gattatgcat tgatcattct ggatattatg cttccgggta tggatggctg
     3481 gcagatctta caaacgttaa gaacggcaaa gcaaacccct gttatttgcc ttactgcaag
     3541 ggattctgtc gatgacagag tcagagggtt ggacagtggg gcaaatgatt atctggtaaa
     3601 acctttttca ttttctgagt tgctagcaag ggttcgggca caattaaggc aacatcacgc
     3661 tctgaattca acattagaaa tcagtggctt aaaaatggac tctgtcagtc aaagtgtcag
     3721 cagggacaat atcagtatta cactgacgcg caaggagttt cagttacttt ggctactggc
     3781 ctccagagct ggcgagatta tacccagaac ggttattgcg agtgaaattt ggggaatcaa
     3841 ctttgatagt gataccaata cggtggatgt cgccattcgc aggctccgcg caaaagttga
     3901 tgatcctttt cctgaaaagc taatcgccac aatccggggg atgggctatt cattcgtagc
     3961 ggtaaaaaaa taatgaaaag attatctata accgtccgtt taaccttgct ttttatattt
     4021 ctgcagtctg ttgctggcgc tggaattgta tggactctct ataatggcct ggcaagtgag
     4081 ttgaaatggc gcgatgatac aacactcatt aaccggacag cgcagatcaa gcagttgtta
     4141 attgatgggg taaatccaga tacgttacct gtgtacttta accggatgat ggatgttagt
     4201 caggatattt tgatcattca tagtgatggc atcaataaaa ttgttaaccg gacaaatgtc
     4261 agtgatgaca tgttaaataa catacctgct agtgagacaa tcagcgcagc aggcatttac
     4321 agaagcatta ttaatgatac agagatagat gctttacgaa ttaacattga tgaagtttcg
     4381 ccatcattaa cggttactgt ggctaaattg gcttcagcca gacataatat gcttgaacag
     4441 tataaaatta atagcattat aatttgcatt gtcgccatta tactgtgctc agtattaagt
     4501 ccgttgttaa tcaggacggg gttgcgagag atcaaaaagt tgagtggcgt aacggaagcg
     4561 ctgaattata acgatagccg agtgccggtt gaggttaacg cattaccgag agaactaaaa
     4621 cctcttgggc aggcgttgaa taaaatgcat catgctttag tcaaagattt tgagcgcctt
     4681 agtcaatttg ctgacgatct cgctcatgaa cttagaacgc caattaatgc attactgggt
     4741 cagaatcagg ttacgctcag tcaaatcaga agtatcgctg aatatcaaaa aacaattgcc
     4801 ggaaacattg aagagctgga aaatatttcg cggttaacag agaatatact gtttcttgcc
     4861 agggcagaca aaaacaatgt tttggtgaaa ctggattcgc tttctctcaa taaggaagtt
     4921 gaaaatcttt tggactatct tgaatatctt tcagacgaga aagagatttg ctttaaggtc
     4981 aagtgcaatc aacaaatctt tgcggataaa attttactgc aacgaatgtt atcgaatctt
     5041 attgttaacg ccattcgata ttcaccagaa aaatcgcgta ttcatataac cagttttctt
     5101 gatgccaacg gctctcttaa tattgatatc gccagtcctg gaacgaaaat taatgagcct
     5161 gaaaaactct tccgtagatt ttggcgggga gataattcgc gtcattccgt aggtcaggga
     5221 cttggccttt ctttagtcaa agcgattgcc gaattacatg gaggaagtgc tacgtatcac
     5281 tatctcagta agcataacgt gttccgaatt acgttaccgc aaagaaatta acatcgggct
     5341 tcagtacgcc ataagttaac cgtgaaaaaa cgcagccacg tcgtgagtat taacgtggct
     5401 gcgattgatt atgcgcttac atcacaatgt aacagggatt aactcgcgta agctgccagc
     5461 atttcctgcg ccgccagttt acccaacgca ttcgctgcaa aaggactgtc gccggtgaga
     5521 cgtttacggt ccttatgtac tcgcccggtg atgtcgtcat taatgatgtt catgcccatt
     5581 ttcttcaact cttcgccgaa gtaccaggtg agatgacccg gcatatagcc aatatctggc
     5641 gtttgtttgt ctgcggcgtc tgggaatgcg caaatagaat aaccattcag tgggttatcg
     5701 ctgtggcgaa gtgccagaaa agccgccgga ccgtggcaaa gggagataac aaaacggtca
     5761 tttttgattg cccactgtaa agcggcagcc acgtcctggc tttctggtaa accaataagt
     5821 gcgccatgcc caccaggaac aaagattgct gcatattcgc tatcagcgtt gaggctggcg
     5881 acaacatccg cgagtttctt cggattacgg aataacgatt tatgctgttc aaagaatggc
     5941 attacttttt catctttgtg cggcatagcc cagtattcaa acttggtcat cagaccggaa
     6001 atggtcgcca cttcgaattc gaaacctgca gcatggagat gatacaacgg cagcaacgtt
     6061 tcaatcggat ggttaccggt cgagaacagt tttccgttat cggtcggcaa ataacgttcg
     6121 tccgcggcga tcaccagaat tttatgttta ccgcgatacg gttttggata gtccacgcca
     6181 tcaagatcag agacgggact cgtatattgg ctaagcgaat attctgaagg gaagaacgca
     6241 ttatcttcag caatatcgac ctgcgggttt ttacttgttt gaacagtcat agtgtattcc
     6301 ttattgttgc ttagtta
//
LOCUS       DAZSIO010000188         6261 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZSIO010000188 DAZSIO010000000
VERSION     DAZSIO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.191"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..624
                     /locus_tag="ACHX5T_003580"
     CDS             <1..624
                     /locus_tag="ACHX5T_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HFR8468608.1"
                     /translation="ILCGLIQATSIILTFNGTRSHAPLGFSFDVMSSIISSNVFLFSF
                     VPWNIANAGWDNHLLSYTLSTLVITLCVFIYIKGNWQMKVFATLPILIVAFSMAKPQL
                     ADSVPQLPTLATGNGSRYFVNIHIAIFSLICVCLFQCIKNKTLKIFFKIYVSVILIAM
                     IKLNFFITPLPDMNWSQGAELINKAKHGEAVSFTVLPPWLTLDLIKK"
     gene            complement(691..2403)
                     /locus_tag="ACHX5T_003581"
     CDS             complement(691..2403)
                     /locus_tag="ACHX5T_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-binding domain-containing protein"
                     /protein_id="HFR8468609.1"
                     /translation="MPEQLYNVVVSQPSQLFTLARSFKANANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHVPVSQPIVINAAGYPVYNGQIAKFVTEQGHSMAVYDAYGSQQFY
                     FPNLLKYDPDQLKQLLQSDEGATYIGTNSGKNLQDELFAIKNEIYQDPLTSWPFGGTL
                     KIKQGVYDVTTPLVLNYDDYPADFVGAPGVRCFYSGENMAETILECRATDFNVKMLGD
                     AAFNSQRLAAYDYIGNMTLKGNPGSYGMLVQNKAHTRLENIVICRHPDGEGLRTDSML
                     TSDMTNVYLQSNRIGWRATNSVNHSELNAITANRLTCSQNSEWGILGDRWGAGTTINS
                     LTCEGNGTQGDSSTGGAQLAINGLNGSCALVLNNPYFEANAGGADLAIDNTGTRPVTV
                     VINGGNFHRVSSARYTHTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSADRPYWITGA
                     NCEVVDIGCVFTETTSKATSASALSVTRSGKINANGSIDVATGVSSVNVVATGVYEVS
                     FSHPLAAVASGYIVQITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSI
                     TRLL"
     gene            2468..2926
                     /locus_tag="ACHX5T_003582"
     CDS             2468..2926
                     /locus_tag="ACHX5T_003582"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR8468610.1"
                     /translation="MEVLYYNKETGIFTWKKKLSARGAVGKVAGTISYGYNAINIDGV
                     RYFAHRLAWLYVYGEWPKQEIDHIDRNRRNNAISNLRDVSRVVNALNVGPRNSNAGIK
                     GVTFCQARNQWQAQINVSGKNITLGRFNTIDEAAIAYKAANMVADHLLSR"
     gene            3058..3318
                     /locus_tag="ACHX5T_003583"
     CDS             3058..3318
                     /locus_tag="ACHX5T_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFR8468611.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQRAKMNNRSLNSEIIAAIEES
                     LDKQNSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSS"
     gene            complement(3408..5405)
                     /locus_tag="ACHX5T_003584"
     CDS             complement(3408..5405)
                     /locus_tag="ACHX5T_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR8468612.1"
                     /translation="MAKAWKDVIASPQYQALAPEQKAQAQEQYFNEVVAPQAGENAEQ
                     AKQAFYAAYPLPSVQPVETQQPVAQQQPQQSGFMSDLGEAVKETGRGLVQAGVNVANI
                     PASVADAVTSAAAWAGGKLGIGDGTYQPAPRVTTHGLEQDFGLQQGALTPQTTEGRVF
                     AEALPYLTPAGVERAATQAPTLAGRIAQGATRLLAENAVGSLAANSAKDDAEALATDL
                     GVGVLAGGAINAAGRGLGAAYRGVRGAIAPEAQQAIRFAEREGVPLHTTDLLQPTSRV
                     GKMAQTTAENIPLAGTSGMRATQQEARSQLVQRFADKFGEYDPAVVIDSLKAKTSGIR
                     RAAGNRLEQVQNAMAGVNIQPARAIQQIDTEISNLQKLGKVADNETISKLQSYRDELV
                     RNAGPEGPVNLDLKQLSDLRSQFRMDVKGERPVLPNRSDAAIQRVYKAMTDDINGAIG
                     QNLGNDTLRKYQQANAVYADEAAKLKNTRLKNVLMKGDLTPEVVNNMLFSKNKSEIKT
                     LYNSVGRVGRVQMRNGIIGKAMEKSGGSPDQFLRQLNILQNQTGITFKGQDAAYLKGL
                     KNYLQSTQQAAKAAVTTPTGQQTIPFIIGYGTAMNPATTGAAVSYGLLTRAYESEPFR
                     NAMLRMANTPRGSTAFEKAMQQAQKAINALTQGAKSDALSE"
     gene            complement(5405..>6261)
                     /locus_tag="ACHX5T_003585"
     CDS             complement(5405..>6261)
                     /locus_tag="ACHX5T_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFR8468613.1"
                     /translation="ELTRVGVDPNNVAQMYQQNPSGFGEFVDHLGMAALGPIDYFNVQ
                     DKMAGREIDRGRLAETIRSNQAGEALTARGQDIQIRGQNISAQNAALSREIQRAELQE
                     KALDRQIARESNQLKLEELKQKQADVRQKAEIARADRQAAAQGAVDTFSTALDSLNEI
                     EQSPGLSKAVGIRSAFPTVPGSDAANFEARLDTFKAQTFLPMVQSLKGMGALSDAEGK
                     KLSDAVGALSPKMSEKAFRDSIGKIRNQLESKLSTVKKQFDYQEPVQNTPGQQSPAGS
                     NFSSLWGD"
BASE COUNT         1628 a         1497 c         1353 g         1783 t
ORIGIN      
        1 atcttgtgtg ggctgataca ggcaacatca ataattctta catttaatgg aacaagatca
       61 catgctccat taggattcag ttttgatgtt atgtcatcta taatatcatc taatgtattt
      121 cttttttcat ttgtcccttg gaatatcgct aatgctggat gggacaatca ccttttatcg
      181 tacacactat caactttagt aattacatta tgtgttttca tttatattaa aggaaattgg
      241 caaatgaaag tgtttgccac ccttccaata cttattgtcg cattttctat ggcaaaacct
      301 caactggcag attctgttcc tcaactgcca acacttgcaa ccggaaacgg gtcaaggtat
      361 tttgtaaata ttcatattgc aattttttca ttaatatgcg tttgtttgtt tcaatgcata
      421 aagaataaaa ccttaaaaat attctttaaa atctatgttt cagtcatcct tattgcaatg
      481 ataaaattaa acttttttat aacccctctt cctgacatga actggtctca aggagcagaa
      541 ttaataaata aagcaaaaca tggagaagcc gtctcattta cagtgctccc tccatggtta
      601 actcttgatt taataaaaaa ataaaacttc acattaataa ataacaatga atccgcctcc
      661 ctacgtattc ccgtagggag gctgtcgtgt ttatagtagc ctggtaatgc taaatgcaaa
      721 cgaactagag attcctgctc cgctaagcgt attgcgcagg gtcaccctaa atgtatctac
      781 gccaatataa gtaacatcac aactaactga atcaggcgca gatataggtg ttatctgcac
      841 aatataaccg ctagcaactg ctgcgagcgg atgagaaaag ctaacctcat acacacctgt
      901 agcaaccacg ttaaccgatg aaacaccagt agcaacgtcg atactaccat tggcgttaat
      961 ttttcccgag cgtgtgactg acaatgcaga agccgatgtt gccttactgg ttgtttctgt
     1021 aaaaacacac ccaatgtcaa ctacctcgca attagctcct gttatccagt atggtcgatc
     1081 tgcacttggc tgataatccc ctgcgctctg gaacgttgtg ccgttgagta tgactgtaac
     1141 ttttccccca cctgagcttg ttacttgaat attagtgtgc gtgtatcttg cactgctcac
     1201 gcgatgaaaa tttccaccgt ttataaccac agttacaggg cgcgtcccag tattatctat
     1261 agcaaggtct gcccccccgg cattagcctc aaagtatggg ttgtttaata ccaaagcgca
     1321 cgaaccgtta aggccattta tggctaattg cgcaccacct gtactggaat ccccctgagt
     1381 accatttcct tcacaagtca ggctattgat agttgtacca gcaccccagc ggtccccgag
     1441 gataccccat tcactgttct gactgcacgt aagcctattt gcagttatag cgttcagttc
     1501 agaatgattt acggagttgg tagcacgcca accgatacga ttactctgca agtacacgtt
     1561 cgtcatgtcg ctcgtgagca tcgagtcagt gcgtaaccct tccccgtcag ggtggcggca
     1621 gataacaatg ttttcaagac gagtatgtgc tttattctgc accagcatac cgtaacttcc
     1681 gggattccct ttcagcgtca tgttgccgat gtagtcatat gcagccaatc tctgactgtt
     1741 aaacgctgcg tcacctagca tttttacgtt aaaatcagta gctctacatt ccagtattgt
     1801 ctcagccatg ttctcgcctg agtaaaaaca gcggacaccc ggagcaccta caaagtcggc
     1861 agggtaatca tcataattaa gtaccagagg agtggtaaca tcatacaccc cttgctttat
     1921 ttttaatgtt ccaccgaaag gccacgatgt aagcgggtct tgatatattt cattttttat
     1981 tgcaaatagc tcatcttgaa gatttttccc gctattagtg ccaatgtaag tagccccttc
     2041 atcagattga agtaactgct ttaactgatc cgggtcatac ttcagcaaat tcggaaaata
     2101 gaactgttgc gaaccatacg catcatatac agccattgaa tggccttgct cagttacaaa
     2161 tttggcaatc tgcccgttgt acacaggata tccggcagca ttgataacaa ttggctgaga
     2221 aacagggacg tgagatccgt cttcattctc tacataaacc tgaatctggt tttcaggatt
     2281 taccgggtca gtgtcaattt tcccgatata aatttttcca ttggcatttg ctttaaacga
     2341 gcgagctaaa gtaaataact gacttggttg tgaaacaaca acgttgtata attgttctgg
     2401 cataataaac attccggaga aaataatgag taagagacgt cacgaggtag taactcggga
     2461 gcgccttatg gaggttctct attacaataa agaaacagga attttcactt ggaaaaagaa
     2521 attaagcgcc agaggtgctg ttgggaaagt tgctggcaca atttcttatg gatataacgc
     2581 aattaatatt gatggcgtaa gatattttgc acatagactt gcctggctgt atgtatatgg
     2641 cgaatggccc aaacaagaga ttgaccacat tgatagaaac agaagaaata acgcaatctc
     2701 aaaccttagg gatgtcagca gagtcgtaaa tgcattaaat gtcggacctc gaaatagcaa
     2761 tgctggaatc aagggggtaa ctttctgtca agccagaaat caatggcaag ctcagataaa
     2821 tgtctcaggt aaaaacataa cccttgggag attcaacacc attgatgaag cggctatagc
     2881 ttataaggca gcaaacatgg ttgctgacca tctgttgagt aggtgatatc tgacatttca
     2941 ttgctccaga cgaatgatat gatgcaacca tgatgtgatt gcataccgaa atggtactat
     3001 tgagtattta tccagtaagt tacgatgcca ttccacccaa ttggtgaggc atcaaggatg
     3061 tacagcaaat acgacgaggc gcagtttcac ttgagacttc cgcatgaact ccacgcgaaa
     3121 attaagcagc gtgcgaagat gaataacagg tctctgaact cagagataat tgcagcgatt
     3181 gaagaatcac tggataaaca aaactctgca tcagtttaca ttgacgatgc agagcgtatg
     3241 gcagaacaac aatccgatat ggttaagaaa attgtctttg atacgctcaa agagctatat
     3301 aaaaaagaca gcagctaacc atcagttacg gaggatttat gcaaagagat atgctgaata
     3361 ttgcgttcta catatttggt ttttgcacgt tcctggtgtt tgcgaagcta ttctgacaac
     3421 gcatcagact tggcaccctg agtcagggcg ttaatggcct tttgcgcctg ctgcatggct
     3481 ttctcaaacg ctgttgatcc gcgtggggta tttgccattc ggagcattgc atttctgaat
     3541 ggctcgctct cataggcgcg agtaagaagt ccgtagctta ccgctgcgcc agttgtcgcc
     3601 gggttcattg ccgtcccata cccgataatg aacgggatgg tttgctgccc tgttggtgtt
     3661 gttactgccg cttttgctgc ctgctgagtg gactgaaggt aatttttcaa tcctttcaga
     3721 taagcagcgt cctgcccctt aaatgtgatg ccagtctggt tttgcaggat gttaagctgc
     3781 cgaaggaact ggtcagggga tccgccagat ttctccatcg cctttccaat gatgccattg
     3841 cgcatttgca ccctgccaac acgaccaact gagttataca gcgtcttaat ttccgatttg
     3901 ttcttgctga atagcatgtt gttgacaact tccggcgtca ggtcgccttt catgagaaca
     3961 ttcttcagcc tggtattctt tagtttcgcc gcttcgtcag cgtagacggc attggcctgc
     4021 tgatatttac ggagagtatc gttgccaaga ttctgaccaa tggcaccatt gatatcgtcg
     4081 gtcatcgcct tgtaaacgcg ctgaatggca gcatcggaac ggtttggtaa cactggtcgc
     4141 tcacccttca cgtccattct gaactgactg cgcaggtcgc ttaattgctt caaatccaga
     4201 tttaccggac cttcagggcc agcattgcga acaagctcat cacgatatga ctgaagtttt
     4261 gaaattgtct cgttatcagc caccttacca agcttctgca ggttagatat ttctgtatca
     4321 atctgctgaa ttgctcgcgc aggctgaatg tttactccag ccatagcatt ctgaacctgc
     4381 tccagtcgat taccggcggc acgacgaatt cctgatgttt tcgctttaag gctgtcaata
     4441 acaaccgctg gatcatactc cccgaattta tcagcaaatc tctgcaccaa ctggcttctc
     4501 gcttcctgtt gcgttgctct cattccgctt gtgccagcca gagggatatt ttctgctgta
     4561 gtctgcgcca tttttccgac gcgggaagtt ggttgtaaca ggtctgtggt gtgcagagga
     4621 actccttcac gctctgcaaa tctgatagcc tgctgagctt ctggcgcgat agcaccacga
     4681 acgccacgat aagcagcacc taatccacgt ccggcagcgt taatagcacc gccagcaagt
     4741 acaccaacgc ctaaatcggt ggcgagtgct tccgcatcat ctttcgcact atttgcagca
     4801 agtgatccaa ctgcgttttc agcgagaagg cgagttgcac cctgagcaat tcgaccagca
     4861 agtgttggtg cctgtgttgc cgctctctca acgccggcag gagtgaggta aggcaatgct
     4921 tcagcaaata ccctgccctc tgtcgtttgt ggagtcagcg caccttgctg aaggccaaag
     4981 tcctgctcta atccatgcgt tgttactcgt ggcgctggtt gatatgttcc atcgccaatg
     5041 ccgagtttac cgccagccca tgcagccgcg cttgttacag catcggcaac cgatgcaggt
     5101 atgtttgcca cgttcacgcc agcctgcacc agtccgcgac cagtctcttt tactgcttcg
     5161 ccaagatcag acataaatcc actttgctgt ggttgttgct gtgctactgg ttgctgtgtc
     5221 tccactggct gcacagatgg caatggatag gcagcataga aagcttgctt agcctgctct
     5281 gcattttctc cggcttgcgg ggcaacgact tcattgaagt attgctcctg agcctgcgct
     5341 ttttgttctg gtgctaacgc ctgatactgt ggagatgcga taacatcttt ccatgcttta
     5401 gccattaatc accccatagt gaagaaaaat tactgccagc aggagattgt tgccctggcg
     5461 tattctgcac cggctcctgg taatcaaact gttttttaac agtgctcaac ttgctttcaa
     5521 gctgatttcg aatctttccg atagagtcac gaaaagcctt ttcactcatt ttgggactta
     5581 gagcaccaac cgcatcggat aattttttac cctcagcatc tgaaagagcc cccatcccct
     5641 tcagggactg caccataggg aggaaagttt gagctttaaa ggtgtcgagt cttgcttcaa
     5701 agttagccgc atcagatcca ggaactgtcg gaaacgctga gcgaatacca actgcttttg
     5761 aaagtccagg gctttgctct atctcgttga gagaatcaag cgcagtgctg aacgtatcaa
     5821 cagcgccctg agcggcggcc tgtctgtcag cacgagctat ttcagccttt tgccgaacat
     5881 ctgcctgttt ctgctttagc tcttcaagct ttaactgatt gctttctctg gctatctgtc
     5941 tgtccagagc cttttcttgt aattctgctc tttgtatttc gcgggaaaga gcagcattct
     6001 gtgcgctgat gttctggcca cgtatctgga tgtcctgacc tcgtgctgtt agtgcctcgc
     6061 cagcctgatt gctgcggatt gtctctgcca gcctacctcg gtcaatctca cgaccagcca
     6121 tcttgtcctg aacattgaag taatcaatcg gaccaagagc agccatccca aggtgatcaa
     6181 caaactcacc aaatcctgaa ggattctgct gatacatctg agcaacgttg ttaggatcaa
     6241 cgccgacgcg agtcagttcc t
//
LOCUS       DAZSIO010000189         6173 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZSIO010000189 DAZSIO010000000
VERSION     DAZSIO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.192"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..856)
                     /locus_tag="ACHX5T_003586"
     CDS             complement(<1..856)
                     /locus_tag="ACHX5T_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR8468614.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNV"
     gene            964..1242
                     /locus_tag="ACHX5T_003587"
     CDS             964..1242
                     /locus_tag="ACHX5T_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468615.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="ACHX5T_003588"
     CDS             complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="ACHX5T_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR8468616.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="ACHX5T_003589"
     CDS             complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="ACHX5T_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR8468617.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3396..3728
                     /gene="hdeA"
                     /locus_tag="ACHX5T_003590"
     CDS             3396..3728
                     /gene="hdeA"
                     /locus_tag="ACHX5T_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR8468618.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3844..4170
                     /gene="hdeB"
                     /locus_tag="ACHX5T_003591"
     CDS             3844..4170
                     /gene="hdeB"
                     /locus_tag="ACHX5T_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR8468619.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4234..4880
                     /locus_tag="ACHX5T_003592"
                     /pseudo
     CDS             4234..4880
                     /locus_tag="ACHX5T_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4922..5452)
                     /locus_tag="ACHX5T_003593"
     CDS             complement(4922..5452)
                     /locus_tag="ACHX5T_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR8468620.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5608..>6173)
                     /gene="slp"
                     /locus_tag="ACHX5T_003594"
     CDS             complement(5608..>6173)
                     /gene="slp"
                     /locus_tag="ACHX5T_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR8468621.1"
                     /translation="NMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQP
                     GLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFLD
                     PVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYGY
                     GAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         1793 a         1295 c         1346 g         1739 t
ORIGIN      
        1 atacattctg gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt
       61 caccatttgg gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg
      121 tcgggtcgga gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga
      181 gctgtactgg cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca
      241 tgcgtaagaa atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta
      301 cggtaaccag cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt
      361 tcccgctgac gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct
      421 gttcaacagc cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg
      481 cggtgtcgta atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa
      541 aggtgatgcg ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt
      601 ttagctcggc ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt
      661 tatcgccttc gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg
      721 caacttcata aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg
      781 agagtgtgac gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat
      841 cgcaggcggt gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt
      901 tcattttagt ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga
      961 gatatgaggg atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac
     1021 aggcacagtg atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca
     1081 gttcctgcca gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat
     1141 caagtcgttt tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc
     1201 cctcgtcatg ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat
     1261 acccagggtg acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt
     1321 ctgaatcttt aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt
     1381 atttttcaga atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt
     1441 cttatggggc aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg
     1501 gctaacaaaa taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac
     1561 aataattctc tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg
     1621 attgtgatag gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga
     1681 cagggaattg atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag
     1741 aaaagaatct ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa
     1801 catgtcatcc ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc
     1861 ctataagaac tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga
     1921 tttatccatc acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt
     1981 gtaaatagta aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata
     2041 accagtttgt gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc
     2101 gagtctcctt gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg
     2161 aaattacctg agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca
     2221 cggatattta tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt
     2281 taaccgccca tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta
     2341 aaacattaaa cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg
     2401 ccatggagtg aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat
     2461 tatttcaatg atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg
     2521 gatcatagtc acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt
     2581 aacgaacaaa ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac
     2641 cagtgtggaa accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat
     2701 gacgatatcc agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg
     2761 ataccaactc atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc
     2821 aatggcaaaa atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc
     2881 gactgcgacg aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa
     2941 cccgacaata agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat
     3001 atcgccagag acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc
     3061 aataaactgg attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt
     3121 tgccttatct atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta
     3181 tcaatctatg ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg
     3241 cagtatctga tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt
     3301 tccatcaaca tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt
     3361 gacggctctt tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc
     3421 ttggtggtct gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg
     3481 ataacaaaaa accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct
     3541 tccagccaac tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg
     3601 cggttttaga tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc
     3661 aggataaaca agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag
     3721 atatgtaatt ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata
     3781 tcttctttaa aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac
     3841 aaaatgaata tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg
     3901 tcactggtga acgcacaatc tgcgttggca gccaatgaat ctgctaaaga tatgacctgc
     3961 caggaattta ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat
     4021 gaagaaacag tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa
     4081 attcctaaag tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa
     4141 aatcaagcat ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt
     4201 gagccatctt gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt
     4261 atactcgcgg caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga
     4321 gcagggttac gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg
     4381 aaatatggtt ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt
     4441 gctgctcagg tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac
     4501 caaaatattg taggtctgac gacggcagcg gatatctggg tgaccgccgc ataggtatgg
     4561 ttattggcag cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc
     4621 tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga
     4681 cattagtgaa cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa
     4741 ctgatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc
     4801 ttcatgccac aacgttgata gaagacctgt tgcgattatt aaaaggtatg gcaggggtga
     4861 aaggcgtttc aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa
     4921 ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga
     4981 ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct
     5041 ccagaatatc ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc
     5101 gcgaaagtga cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa
     5161 aatcaatgat agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt
     5221 taatattaaa gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga
     5281 gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat
     5341 gcaacattac gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa
     5401 tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact
     5461 cctggtgcgg acattaactt tataatcagt ttgccaaagg taattaacag gacagggctt
     5521 ataataataa tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg
     5581 agaaagcatc tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac
     5641 cgcattggtg tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta
     5701 atcccacagg ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg
     5761 catattcact tccaggaagt tatacgggac tttattgata aagcctggtt gttcaccctg
     5821 aatggtaccg aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct
     5881 ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc
     5941 caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac
     6001 cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac
     6061 aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca
     6121 tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tca
//
LOCUS       DAZSIO010000190         6080 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZSIO010000190 DAZSIO010000000
VERSION     DAZSIO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.193"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..143)
                     /locus_tag="ACHX5T_003595"
     CDS             complement(<1..143)
                     /locus_tag="ACHX5T_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HFR8468622.1"
                     /translation="MEQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQ"
     gene            379..651
                     /locus_tag="ACHX5T_003596"
     CDS             379..651
                     /locus_tag="ACHX5T_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFR8468623.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(617..1024)
                     /gene="nudG"
                     /locus_tag="ACHX5T_003597"
     CDS             complement(617..1024)
                     /gene="nudG"
                     /locus_tag="ACHX5T_003597"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR8468624.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1111..1749
                     /locus_tag="ACHX5T_003598"
     CDS             1111..1749
                     /locus_tag="ACHX5T_003598"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR8468625.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1758..3065)
                     /gene="ynjE"
                     /locus_tag="ACHX5T_003599"
                     /pseudo
     CDS             complement(1758..3065)
                     /gene="ynjE"
                     /locus_tag="ACHX5T_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3132..3785)
                     /locus_tag="ACHX5T_003600"
     CDS             complement(3132..3785)
                     /locus_tag="ACHX5T_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR8468626.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3785..5256)
                     /locus_tag="ACHX5T_003601"
                     /pseudo
     CDS             complement(3785..5256)
                     /locus_tag="ACHX5T_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5229..6080)
                     /locus_tag="ACHX5T_003602"
                     /pseudo
     CDS             complement(5229..6080)
                     /locus_tag="ACHX5T_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1427 a         1624 c         1609 g         1420 t
ORIGIN      
        1 atctggcgat attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga
       61 acggcttgcg cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac
      121 tccagagaat atgtctgctc catagatata aaaaccttat atattaatac gattgtgctt
      181 atgttgttta tgtcatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta
      241 agcaagaaaa aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat
      301 gcgataactc agacgctaaa gttttgccac ccggctaccg ttatcagtag cagagacaat
      361 aacgttaagg agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta
      421 attgcactgg ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg
      481 ttcagttcag gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggatcaa
      541 aattattctg aaggggcggt aattaaagcc gaaggaatac tgctgcaatg ccagcgtgat
      601 gacaaaaccc ttagcactaa tccgctgctc tggcggcgcg taaagccata aacgcctcta
      661 ataatggaat gtcagcaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga
      721 ccagcgcctg atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat
      781 gaagatggat aatccggccc gaaacttctc gctgatggct ggcaacatac tcacccacag
      841 ttgcttcgat gtccaattct tcgtttaact cacgcaccag cgcctgctgc tggctttcat
      901 ccagctcgac tttacctccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc
      961 gttgcgcgag taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt
     1021 tcataaatta cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa
     1081 tcagaaaggc tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt
     1141 aaaccgttgt tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt
     1201 ttaacattag tgggatttgc catcggcgtg ctggctctgc cgtttctggc gctgggctgg
     1261 tatctggcgg cgctagtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg
     1321 ctggcgcggc gcagagggct taccgacgcg ggcggctttc ttgatatttc tctcgatttt
     1381 ctcttttacg cgctggtgcc gtttggcttt attcttgctg caccggagca aaatgcgctg
     1441 gcaggcggct ggttgttgtt tgcgtttatc ggcacgggca gcagttttct cgcctttgct
     1501 gcactggcag caaaacatca gattgataac cccggttatg cacataagtc gttttactat
     1561 ctggggggat taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc
     1621 cctgcatggt ttgcgtggtt tgcgtggttt gcgtggtttg cgtggatctt cggtgcgttg
     1681 tgctggatga cgacctttac gcgggtgtgg agtgggtatc tgacgctgaa gtcgctccag
     1741 cgtcagtaat tgcaatgtta tttgctactg cccgggccgc gttcaccggt tgcaaccgga
     1801 ttttttggat cgctgctcca ttcgtaccag ccgccgtcat acacggaaac attcttccaa
     1861 cccatggcgc gtgcgtacat aaaggtttcg gacgcgcgcc agccggtgcc gcagtagaat
     1921 gaaacttgct gatctggttt gatattccat gctttccaca tagcggtaat atcatcggcg
     1981 ctacgcatgg tgccatccgg gttatagaaa tcttccatat gcgtcgagtc gctaccagcg
     2041 tgtccccaac gtgctccggc tatttcacct tttggtttaa tatagctgta accgctggtc
     2101 gtaccgataa attctggcca cgaacgaatg ctcaccagcg atgcatcctg gcgatgcagc
     2161 agtccacggg cctgttccat atcgagcatc aactgcggtt gtgccgggat cttcacgccg
     2221 aaatccggtt ccgctttcac tttcggtggc gttcctcgct caacaggcag tcccgcgtcg
     2281 gaccaggttt gccagccgcc atccagcaga cgcacatctt tcacgcctgc gtagagcata
     2341 atttgcgcca cacgcgctcc agcgtatacg tcacgcccgt acagaatgac cgtggtgtca
     2401 tggcgaatgc cgtgttttgc cagcatcgct tttagttgtt catcagaaac tttgttccac
     2461 agcggttcgc tttccacttc gttggtatcg atgtagtcag cgccaggaat atggctgata
     2521 aggtaaagct taggagcgcc ctaggccgct tcaatgactt tccagtcacc gacaggtttc
     2581 gccgtaacct ctttaccttg ttgcaggtca tgcagccatt gcggataaac cagctgctca
     2641 aagtgcggca gtttttgtag acgggaaggt tcgcttagcg cgtcactcag aatggagata
     2701 tgcgtaaaac ctgctttttg cagtcgcgtt ttgacggcgt caacatcttt gccattaccg
     2761 tacagcgcca ccggggcatc ggctttcagg ttatgttgct tgatccacgc gttgagctgt
     2821 tcggtgctca ttttgtccag ccagctagca gagaggttta aggcggcagg ttcatgacca
     2881 gaagggccat ttaaggtttg tggccagccg ttataaaacg cgctggggcg agtatctatc
     2941 gctttgccat tttgttgctg aagttggtca agtgtaagag gcttcgccag ttcagcggcc
     3001 cacgaagaag cgcaagccag ccctaaagcc attgccagcg cggtcatttg agaaacacgt
     3061 ttcatcgaaa atcccgacat tgtagttttg agtgcgccat tctgaactga cgcattgaga
     3121 aaaactttgc gttatcgcag tttgttgtaa ttttctgacc actgcgccat atccagaaca
     3181 gaactatcag caggaacatc ctggagatcg tgcgttacct gaacgacggg gatcgccagg
     3241 gcgcgaactt cgctgaacac ccactggcga aaattatcgc gcagagccac atcaagacgg
     3301 ctgaatggct catccaggag taacgctttt ggctgggcga gaagggcgcg tagtagagca
     3361 acgcgcgctc gctgaccgcc agacaaagtg gcaggatcct gatggaaagc tccgtcaagg
     3421 cctgaacgct caagtgcatc attcacggca ttacgtcggg cattcccctt aagtgtcgcc
     3481 ggtagcgcca gcagtaaatt ttgcccgacg ctgaactggt caaataacag tgcatcctga
     3541 aaaagaatgc caatctgacg ctgtgcggtg ggtagcatgt caatccgttg ctcattgagc
     3601 catagctcac ctgtacaaga aaactgtccg gccagtgcac caatcatcca tgaaaacaga
     3661 gtggattttc cacagccaga cggccccatt aacgtgacga tgtcaccttt atcaaccgta
     3721 aagttaacgt ttgtcagcaa gcggctttct ggtaaacgta gcgaaacatt tttcacgcag
     3781 agcattagcg gattccttgt ctgacataac ctacccattt tgcgactaac gcggtcaggg
     3841 caaaaataat aagcggtaac aacagttgcc ataacgcctg ggcggcgaga ataccgttgc
     3901 tgccgccgct gcttaatgcc accgcctcag tggtgagcgt cggaaaacgc cccgcgccca
     3961 gccacagcgt cggcatatac tgcgcaatac tgactgaaaa tcccaccgcg aaggcaatca
     4021 gcacagggcg caacatgagt gggcatttca cgtaaaagaa gattttggcc cgcgaccagc
     4081 ccagtgtttg cgcaatcaaa attaaccgtg aatcaatgcg ctgccaggcg ggttgcagga
     4141 taaacagcat ccacggcatt acccacagca gatgccccca gaccaccgcc gtccagcttc
     4201 catccaggtt cagccatagc gccagcgtgt actggcccgc caccagtggc agagcgggca
     4261 atagaatggg caaccatagc cacaactggc gacgctgtgc cccccattcc agccacagca
     4321 gtaacaggag caaagcgatg aatgttgcta ccagccccat tgtcagactg ttgatgagtg
     4381 cttcactatt gatcgtcgac tgatccgcga ggatcgccag cagaaccaca cacagcacac
     4441 cggttaaggg taaaaaactc gccagcgtag tgcctggtaa taaaggtgtg gcaggcttgc
     4501 gaatgccatc tacgcgggga atagtgcgcc gccagctgcg ccaaaacgta ggcggcgagt
     4561 aacagcatca gcagcagact ggcgagtgcg cctttcgttt gttgatccgc gtcgccctga
     4621 gttaaccact gccagctaat tactgccagc gtcggtggat tacctggccc aagaataatc
     4681 gccacatcca cgaccgaaag cgaccaggca acaatcgcca gcatcgccat tgccagcggc
     4741 tgtagcccag cgaatccagc acaatgacct gctgcaacag ccgtttttcg ctcaatactg
     4801 ccactaagat ccacagcaga aatgcgcttt ctttcactgc aagggtgagg cccagaccga
     4861 tccaaatcgg tccattggcg gagtgaaata cgggaaatag tcatatagca gccctccgtc
     4921 agcaaagagc agcagggcgc tggtggcaaa agccacatgg ggaatggcga gcagccacgg
     4981 cagacgggcg cacatacgct gccatttcgg cccaggccat agcgccacaa tcaccagcag
     5041 ggcaatcaac aatgccccga ccgccgcgat ggttgtcgac accagcgttg ccagtaatgc
     5101 ctgcggtaac tgtggatcgg caaataacgc ctgccagcgt gtcaacgaca acgcaggcga
     5161 tatcagcgtg agagctgccg ggatcagcgg tgcataaatc accgccacca tcgcccacag
     5221 caaaaagatt aatgcgtacc gtaacggcgt aaccattctt gttccagtgc attcacccat
     5281 cctgcgtgcg gttcagccag taccggcggc agatcctgcg gcattcttga ttgcaatatt
     5341 tcgcgctgcc cgtcaggcag tttttgcgga tcgagaacag aaggatcgcc ccagacaacg
     5401 ggatctgctt tacgcagttg cgcatcgggt gagagcagga aattggcaac taccttcgcc
     5461 gcagcactgg cattcgcgtt ggctggaatg gtgacgaaat gcacgttgcc aatcatcccc
     5521 tcgcgaaagc caaaactgta actgcttgcc ggcaaatcgc cgctggcgat tttttgctgc
     5581 gcatgtgcgg ggttaaaggt cagcgacagg cgcaatgtgc cggctttcag cagagcatcc
     5641 atccgcgcgg gcgaaggcgg gaaatccttt ccttcgcgcc acaaatacgg atgcagtgca
     5701 tcaagatatt gctacaaggg cgcagtgaca cgggcgaaag tcgcatcgtc cggcgcttct
     5761 tttaatgcgg cgggatctgg cgtcagcata atcagcaact gttcaagaaa cgccgtgccg
     5821 gtaaagtccg gtgggcgcgg ataagtaacc gtgccgggat tggctttagc aaactccagt
     5881 aaggcttgcg gcgtttgtgg tggctgtggc gtaacatcgc ggcgggcgat aaacgtcagt
     5941 tgtgcgccgc cccagggcga ttccgcacct tgtgtcggca ctgaaaaatc tccccgcacc
     6001 ggcagctgtg tgtcgacata gcgccagttg ggcagagtct ccgcccagcc cgtttgtaat
     6061 aaattggcct cttttaaggt
//
LOCUS       DAZSIO010000191         6029 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZSIO010000191 DAZSIO010000000
VERSION     DAZSIO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.194"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            183..1304
                     /gene="ompC"
                     /locus_tag="ACHX5T_003603"
     CDS             183..1304
                     /gene="ompC"
                     /locus_tag="ACHX5T_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR8468627.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1416..2471
                     /gene="apbE"
                     /locus_tag="ACHX5T_003604"
     CDS             1416..2471
                     /gene="apbE"
                     /locus_tag="ACHX5T_003604"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR8468628.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2545..3609
                     /gene="ada"
                     /locus_tag="ACHX5T_003605"
     CDS             2545..3609
                     /gene="ada"
                     /locus_tag="ACHX5T_003605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR8468629.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3609..4259
                     /gene="alkB"
                     /locus_tag="ACHX5T_003606"
     CDS             3609..4259
                     /gene="alkB"
                     /locus_tag="ACHX5T_003606"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR8468630.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4335..5978
                     /gene="yojI"
                     /locus_tag="ACHX5T_003607"
     CDS             4335..5978
                     /gene="yojI"
                     /locus_tag="ACHX5T_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR8468631.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1430 a         1511 c         1645 g         1443 t
ORIGIN      
        1 gtatttacat tttgaaacat ctatagcgat aaatgaaaca tcttaaaagt tttagtatca
       61 tattcgtgtt ggattattct gcatttttgg ggagaatgga cttgccgact gattaatgag
      121 ggttaatcag tatgcagtgg cataaaaaag caaataaagg catataacag agggttaata
      181 acatgaaagt taaagtactg tccctcctgg tcccagctct gctggtagca ggcgcagcaa
      241 acgctgctga agtttacaac aaagacggca acaaattaga tctgtacggt aaagtagacg
      301 gcctgcacta tttctctgac gacaagtctg tagatggcga ccagacctac atgcgtcttg
      361 gcttcaaagg tgaaactcag gttactgacc agctgaccgg ttacggccag tgggaatatc
      421 agatccaggg caacagcgct gaaaacgaaa acaactcctg gacccgtgtg gcattcgcag
      481 gtctgaaatt ccaggatgta ggttctttcg actacggtcg taactacggc gtagtttacg
      541 acgtaacttc ctggaccgac gtactgccag aattcggtgg tgacacttac ggttctgaca
      601 acttcatgca gcagcgtggt aacggcttcg caacctaccg tagcaccgac ttctttggtc
      661 tggttgacgg tctgaacttc gctgttcagt atcagggcaa aaacggtagc ccggaaggtg
      721 aaggcatgac caacaacggt cgtgaagctc tgcgtcagaa cggtgacggc gttggcggtt
      781 ctattactta tgattacgaa ggcttcggta tcggtgctgc agtttccagc tccaaacgta
      841 ctgatgacca gaacttcggt ctgaaccgtt atgatgagcg ttacatcggt aatggcgacc
      901 gcgctgaaac ctacaccggt ggtctgaaat acgacgcgaa caacatctac ctagctgctc
      961 agtacaccca gacctacaac gcaactcgcg taggcaactt gggttgggcg aacaaagcac
     1021 agaacttcga agctgttgct cagtaccagt tcgacttcgg tctgcgtccg tccctggctt
     1081 acctgcagtc taaaggtaaa aacctgggtg tcatcaatgg tcgtaactac gacgacgaag
     1141 atatcctgaa atatgttgat gttggtgcga cctactactt caacaaaaac atgtccacct
     1201 acgttgacta caaaatcaac ctgctggacg acaaccagtt cactcgtgac gctggcatca
     1261 acactgataa catcgtagct ctgggtctgg tttaccagtt ctaatctcga tggatatcga
     1321 acaaagggcc tgcgggccct tttttcattg ttttcagcgt acaaacacag ttttttggtg
     1381 tactcttgcg accgttcgca tgaggataat cacgtatgga aataagcttt acccgcgtgg
     1441 cactgctggt tgccgcgctc ttctttgttg gttgcgatca aaaaccacaa cccgccaaaa
     1501 cccacgctac tgaagttacc gttcttgaag gcaaaaccat gggtaccttc tggcgtgcca
     1561 gcatcccggg cattgatgcc aaacgcagtg ccgaacttaa agaaaagatt cagacccagc
     1621 tggacgccga cgatcagctg ctttcgacct ataaaaaaga ttccgcgctg atgcgcttta
     1681 acgactcgca aagtttgtcg ccgtggccgg taagtgaagc gatggccgat atcgtcacca
     1741 cctcgctgcg cattggggca aggaccgatg gtgcgatgga tattaccgtc gggccgctgg
     1801 tgaatctgtg gggctttggc ccggaacaac agccggttca aattccgagc caggaacaga
     1861 tcgatgcgat gaaagccaaa accggcttac agcacctgac ggtcatcaat cagtcgcatc
     1921 agcaatatct gcaaaaagac ctgccggatt tatatgtcga tctctccacc gtcggtgaag
     1981 gttatgcggc ggatcacctg gcacgcttga tggagcagga agggatttcc cgctatctgg
     2041 tgtcggtggg cggcgcgctg aacagccgtg gtatgaacgg tgaaggccag ccgtggcggg
     2101 tagcgattca aaaaccaacc gataaagaaa acgcggttca ggctgtggtg gatatcaacg
     2161 gtcatgggat tagcacctct ggcagctacc gtaactatta cgaactggac ggcaaacgtc
     2221 tttcccatgt tatcgatccg caaaccgggc gtcccatcga acacaatctg gtatccgtga
     2281 cggtgattgc tccgacggcg ctggaagccg atgcctggga tactggcttg atggtactcg
     2341 ggccggagaa agccaaagag gttgttcgcc gggaagggct ggcggtttat atgatcacca
     2401 aagaaggcga tagctttaaa acctggatgt caccacagtt taaaagcttc cttgtcagcg
     2461 aaaaaaatta aagcgcaaga ttgttggttt ttgcgtgatg gtgaccgggc agactaaagg
     2521 ctatccttaa ccagggagct gattatgaaa aacgccacat gcttaactga cgatcaacgc
     2581 tggcaatctg tcttagcccg cgacccgaat gccgacggcg aattcgtttt cgccgtgcgc
     2641 accacgggta tcttttgccg tccttcttgc cgcgccagac atgccttgcg ggaaaatgtc
     2701 tccttctacg caaatgccag cgaggcactc gccgccggat ttcgcccctg caaacgttgt
     2761 cagccagaca aagccaatcc ccggcaacat cggttggata aaatcaccca cgcgtgtcga
     2821 ctgctggaac aggaaacgcc tgtaacgctg gaagccttag ccgaccaggt ggcgatgagt
     2881 ccatttcatc tacatcggtt gtttaaagct actaccggaa tgacgcctaa agcctggcaa
     2941 caggcctggc gcgctcgccg tttgcgcgaa tcgctggcga aaggggagag cgtgacgacg
     3001 tctattctta acgccggatt ccccgacagc agcagttact accgcaaagc cgatgaaacg
     3061 ctgggcatga cggctaaaca attccgtcac ggtggcgaaa atctggcggt gcgttacgcg
     3121 ctggctgatt gtgagctggg tcgttgcctg gtggcagaaa gcgagcgggg gatttgcgcg
     3181 atattgctgg gcgatgatga cgcgacatta atcagcgagt tgcagcagat gtttcccgct
     3241 gccgacaacg cgcctgccga tctgacgttt cagcaacatg tgcgtgaagt gatcgccagc
     3301 ctcaatcaac gcgatacgcc gctgacgtta ccgctggata ttcgcggcac tgcttttcag
     3361 caacaagtct ggcaggcact gcgcacgata ccttgtggtg aaaccgtcag ttatcagcaa
     3421 ctggctaacg ccatcggcaa accgaaagcg gtacgggccg ttgccagcgc ctgtgccgcc
     3481 aacaagctgg ctatcgtaat accctgtcat cgggtggtcc gtggtgatgg cacactttcc
     3541 ggttaccgct ggggcgtgtc gcgtaaagcg caactgctgc gccgcgaagc tgaaaatgag
     3601 gagaggtaat gttggatctg tttgccgatg ctgaaccgtg gcaagagcca ctggcggctg
     3661 gtgcggtaat tttacggcgt tttgctttta atgctgcgga gcaactaatc cgcgatatta
     3721 atgacgttgc cagccagtcg ccgtttcgcc agatggtcgc tccaggcgga tataccatgt
     3781 cggtggcgat gaccaactgt gggcatctgg gctggacgac ccatcggcaa ggttatctct
     3841 attcgcccat tgatccgcaa acaaataaac cgtggcccgc tatgccacaa agctttcatg
     3901 atttatgtca acgtgcggct acggcggcgg gctatccaga tttccagcca gatgcttgtc
     3961 ttatcaaccg ctacgctcct ggcgcgaaac tgtcgctgca tcaggataaa gacgaaccgg
     4021 atctgcgcgc gccaattgtt tctgtttctc tgggcttacc cgcgattttt caatttggtg
     4081 gcctgaaacg taatgatccg ctcaaacgtt tgttgttgga acatggcgat gtggtggtat
     4141 ggggcggtga atcgcggctg ttttatcacg gtattcaacc gttgaaagcg gggtttcatc
     4201 cactcaccac cgactgccgc tacaacctga cattccgtca ggcaggtaaa aaagaataaa
     4261 aataagaatt attattgctg tgcgcgcgaa gatgtttaaa ctgcgggctg taattcattg
     4321 tccgggtttt ctgcatggaa cttcttgtac ttgtctggcg gcagtatcgc tggccattta
     4381 tcagtgtgat ggcgctaagc ctcgccagtg cggcattagg cattggctta attgctttta
     4441 tcaatcagcg ccttatcgaa acggcggata ccagtctgct ggtgttaccg gagtttctgg
     4501 gattattgct gctgttgatg gcagtcactc tcggatcgca actggcgctc accactttgg
     4561 ggcatcactt cgtttaccga ctgcgtagtg aatttatcaa gcggattctg gatacccacg
     4621 tcgagcgcat tgaacaactc ggtagcgcct cgttgctggc gggattaacc agcgatgtgc
     4681 gcaatatcac cattgctttt gtgcgtctgc cggaactggg gcaggggatc attctcacta
     4741 tcggttcatc ggcgtatctg tggatgctgt cgggcaaaat gttgctggta acggctatct
     4801 ggatggcgat caccatctgg ggcggttttg tgctggtggc gcgggtgtac aaacatatgg
     4861 cgaccctacg tgaaaccgaa gacaagctgt acacggattt tcaaactgta cttgaagggc
     4921 gcaaagagct gactctgaac cgggaacgcg ccgagtatgt gtttaacaac ctctacatcc
     4981 ccgatgcgca agagtatcgc catcatatta ttcgcgcaga caccttccat cttagtgccg
     5041 tgaactggtc aaacatcatg atgctgggcg ctatcggcct ggtgttctgg atggcgaaca
     5101 gtctcggttg ggctgatacc aacgttgctg cgacctattc gttgacgctg ttattcctgc
     5161 gtacgccgct gctttcggcg gttggcgcat tgccgacgct gctgacggcg caggtggcgt
     5221 ttaacaagct gaacaaattc gcgctcgcgc ctttcaaagc tgagtttccg cgcccgcagg
     5281 cgtttcccaa ctggcaaacg ctggagctgc gtaacgtgac gtttgcttat caggataacg
     5341 cgttttccgt tggtccgatt aatctcacca tcaaacgtgg cgagctgctg tttctgattg
     5401 gcggtaacgg tagcggaaaa tcgacgctgg cgatgttgtt gacgggcttg tatcagccac
     5461 aaagcggcga aatcttgctg gatggcaaac ctgtcagcgg cgaacaaccg gaagattatc
     5521 gcaaactgtt ttcggcagtg tttaccgatg tctggctgtt tgatcaactg ctggggccgg
     5581 aggggcaacc cgctaacccg caactggttg agaagtggct ggcgcagctg aaaatggctc
     5641 ataagcttga gttaagcaac gggcgtattg ttaacctgaa gttatcaaaa gggcagaaaa
     5701 aacgcgtggc gctgttgctg gcgctggcag aagaacgcga tattatcctg ctggatgaat
     5761 gggcggtgga tcaggatcca cacttccgtc gtgagtttta tcaggtgttg ctgccgctga
     5821 tgcaggagat gggtaaaact attttcgcta tcagtcatga tgatcattac tttatccacg
     5881 ccgaccgcct gctggaaatg cgcaatgggc aacttagcga gctgacgggc gaagagcgcg
     5941 atgccgcttc gcgtgatgcc gttgcccgga cggcataata ttacgacgct gacctggcgg
     6001 actgctgccg tcaggtcaat atgctaaaa
//
LOCUS       DAZSIO010000192         5922 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZSIO010000192 DAZSIO010000000
VERSION     DAZSIO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.195"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            105..386
                     /locus_tag="ACHX5T_003608"
     CDS             105..386
                     /locus_tag="ACHX5T_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468632.1"
                     /translation="MQNRKWILTSLVMTFFGIPILTQFFAAVIAMLGAGLAAIPEVCN
                     LLFTPTIYLLLNIFMLTLGALMLFFSGRVGNVPEFCYVGYDGVGFAVIS"
     gene            complement(592..2097)
                     /gene="glpD"
                     /locus_tag="ACHX5T_003609"
     CDS             complement(592..2097)
                     /gene="glpD"
                     /locus_tag="ACHX5T_003609"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR8468633.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANALMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            2287..2613
                     /gene="glpE"
                     /locus_tag="ACHX5T_003610"
     CDS             2287..2613
                     /gene="glpE"
                     /locus_tag="ACHX5T_003610"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR8468634.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMVHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            2658..3488
                     /gene="glpG"
                     /locus_tag="ACHX5T_003611"
     CDS             2658..3488
                     /gene="glpG"
                     /locus_tag="ACHX5T_003611"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR8468635.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            3505..4263
                     /locus_tag="ACHX5T_003612"
     CDS             3505..4263
                     /locus_tag="ACHX5T_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR8468636.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(4245..5843)
                     /gene="rtcR"
                     /locus_tag="ACHX5T_003613"
     CDS             complement(4245..5843)
                     /gene="rtcR"
                     /locus_tag="ACHX5T_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFR8468637.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
BASE COUNT         1331 a         1547 c         1594 g         1450 t
ORIGIN      
        1 ccaaagggtc ccgttttttt cgccatcatt tcggaaaagt gtccagaagg ggcaagggga
       61 ctcggggagt ataatttcgc cattcagaaa gaatggaaag aataatgcaa aatagaaaat
      121 ggattttgac ctcgctggta atgacttttt tcggcatccc aatactgacg caattttttg
      181 cagcggttat tgccatgctg ggggctgggc ttgccgctat tcctgaggtt tgcaatttac
      241 tctttacgcc aacaatttac cttctgctca atatttttat gctgacgctg ggcgcattaa
      301 tgctattttt ctcggggcga gtggggaacg tgcctgagtt ttgttacgtt gggtatgatg
      361 gcgtgggctt tgcggtaatt tcctgaatta aaaagtaggt tagatttttg acaaagtgcg
      421 ctttgttcat gccggatgcg acgtgaacgt cttatctggc ctacaagacc atgcaaattc
      481 aatatattgc ataaaccacg taggcctgat aagcgtagcg catcaggcag ttttgtgttt
      541 gtcatcagtc tcaggccaga ttgaaatctg acctgatcac cttacgttaa tttacgacgc
      601 cagcgacaac ttctgctgcg tatattccac cagccactga ctcacacgag attgttgatc
      661 cgcattcagc cacatgcctt gttttgtgcg acgccacagg gcatcgtcgg tgcggcggac
      721 ccattcgtga tccaccaggt atttcagttc cgcttcgtag aactcatgac cgaaatcttc
      781 cccgagatcg cttaccgttc ccgcattgcc gagcagcagc tcgctgttgc tgccgtaagt
      841 gcgcgtgtag tgacgtgcca gcgattcagt caggaacggg taacggcggc gcaggcgagc
      901 ggcgtaatcg tcgcggtcgc cttcaatggc accacccggc agcacgctct ctttcgtcca
      961 cgccgggcca ataccctgat aatacggcgt cagtttttcc agcgcatgtt ctgccagttt
     1021 tcggtaggtg gtcagcttac cgccgaatac cgacagcagc ggtgctttgc cgttttcatc
     1081 atggatatca agggtgtaat cacgggtaat ggcttgcggc gagtcggact catcatcaca
     1141 cagcggacgc acaccggagt aggtccagac gatatcgtca cggcttagct gctttttaaa
     1201 gtgcgcgtta tacactttca gcaggtaatt gatttcactc tcttcaatct tcactgcttt
     1261 cggatcacct ttgtattcca catcggtggt gccgatgatg gaaaactcgt ccatccacgg
     1321 gatcacgaac acaatgcgtt tatcttcgtt ttgcagaatg taggcctgct tttgggtatg
     1381 cacgcgcggc accacaatat ggctgccttt gatcaggcga atgccataag gcgaaggcag
     1441 atgcatcccc tcgtcgaaga actgtttcac ccacgggccg gtggcgttaa ccaggccgcg
     1501 cgcttgccag ctatattttt tgccggtatc gatatcttcc gcttccacaa tccacaggcc
     1561 gttttcgcgg cgagcagagg tggcgcgagt ccgagtaagc acttcgccgc ctttacgcac
     1621 caccatcagg gcgttggcga gtaccagacg ggcgtcgtct acccaacagt cagaatattc
     1681 gaatccgcgc ttaatttccg gttttaacac tgaatttgcg ccaaaacgca aaccagttga
     1741 tcccggcagg ctggtgcgtt tacccagatg atcgtacata aacagaccaa tgcgaatcat
     1801 ccacgccggg cgcagatgcg gacgatgtgg caggcgaaaa cgcatcggga aggcgatatg
     1861 cggagccatt ttcagcagca cttcacgttc agccagcgcc tcgctgacca ggcggaattc
     1921 atagtgctca aggtagcgca ggccaccgtg aatgagtttt gaactggcgg aagaggtcgc
     1981 gcaagcgaga tcctgcgcct ccagcatcag cacggataaa ccgcgtccag cggcgtctgc
     2041 cgcgatacca gcaccattga tgccgccacc tatcacaatc agatctttgg tttccatgct
     2101 gccctcattc actttcgtta aagctcataa atgttcgtta tcgaacatat tagcaaagaa
     2161 tcgcgctttg ggtaacattg aaaaaacatt ttagagtgat atgtataaca ttatggcgtt
     2221 tatctgccgc ttcgacgtaa actgtgcggt aaatttgccc acttgtttgt aaagaaagag
     2281 agacgcatgg atcagttcga atgtattaac gttgccgacg cgcaccagaa gttgcaggaa
     2341 aaagaggcgg tgctggtcga tattcgcgat ccacagagtt tcgctatggt gcatgcggtg
     2401 caggctttcc atttaaccaa cgacacgctg ggcgcgttta tgcgtgataa cgactttgac
     2461 actccggtga tggtgatgtg ttatcacggc aatagcagca aaggggcggc gcagtatctg
     2521 ctgcaacagg gctacgatgt ggtctatagc attgacggcg gctttgaagc ctggcagcga
     2581 cagtttcccg cagaggtggc gtacggcgcg taacgcttta tactgtcccc ttttgtgtgg
     2641 aataagcgac agcaacgatg ttgatgatta cctcttttgc taacccccgc gtggcgcagg
     2701 cgtttgttga ttacatggcg acgcagggtg ttatcctcac gattcaacaa cataaccaaa
     2761 gcgatgtctg gctggcggat gagtcccagg ccgagcgcgt gcgggcggag ctggcgcgtt
     2821 ttctcgaaaa cccggcagat ccgcgttatc tggcggccag ctggctggca ggccataccg
     2881 gcagtggcct gcattatcgc cgttatcctt tctttgctgc cttgcgtgaa cgcgcaggtc
     2941 cggtgacctg ggtgatgatg atcgcctgcg tggtggtgtt tattgccatg caaattctcg
     3001 gcgatcagga agtgatgtta tggctggcct ggccattcga tccaacactg aaatttgagt
     3061 tctggcgtta cttcacccac gcgttaatgc acttctcgct gatgcatatc ctctttaacc
     3121 tgctttggtg gtggtatctc ggcggcgcgg tggaaaaacg cctcggtagc ggtaagctaa
     3181 ttgtcattac gcttatcagc gccctgttaa gcggctatat gcagcaaaaa ttcagcgggc
     3241 cgtggtttgg cgggctttct ggcgtggtgt atgcgctgat gggctacgtc tggctacgtg
     3301 gcgaacgcga tccgcaaagt ggcatttacc tgcaacgtgg gttaattatc tttgcgctga
     3361 tctggattgt cgccggatgg tttgatttgt ttgggatgtc gatggcgaac ggagcacaca
     3421 tcgccggatt agccgtgggt ttagcgatgg cttttgttga ttcgctcaat gcgcgaaaac
     3481 gaaaataatt ccagggattt ataaatgaaa caaacacaac gtcacaacgg tattatcgaa
     3541 ctggttaaac agcagggtta tgtcagtacc gaggaactgg tggagcactt ctccgtcagc
     3601 ccgcagacta ttcgccgcga cctcaacgag ctggcagagc aaaacctgat cctgcgccat
     3661 catggcggcg cggcgctgcc ttccagttcg gttaacacgc cgtggcacga tcgtaaagcc
     3721 acccagaccg aagaaaaaga gcgcatcgcc cgcaaagtgg cggagcaaat ccccaatggc
     3781 tcgacgctgt ttatcgatat cggcaccacg ccggaagcgg tggcgcacgc gctgctcaat
     3841 cacagcaatt tgcgcattgt caccaacaat ctcaacgttg ctaacacgtt gatggtaaaa
     3901 gaagatttcc gcattattct cgccggtggc gaattacgta gccgcgatgg cgggatcatt
     3961 ggcgaagcga cgctcgattt tatctcccag ttccgccttg atttcggcat tctggggatc
     4021 agcggcatcg acagcgacgg ctcgctgctg gagttcgatt accacgaagt tcgtaccaag
     4081 cgcgccatta ttgagaactc gcgccacgtt atgctggtgg ttgaccattc aaaatttggc
     4141 cgtaatgcga tggtcaatat ggggagcatc agcatggttg atgccgtcta caccgacgcc
     4201 ccgccgccag taagcgtgat gcaggtgctg acggaccacc atattcaact ggagctgtgc
     4261 tgatcctgca cggcttccca cgtcagacca aaacgcgcca ggtatttgcg tagccgatcc
     4321 gcgtcattga cgctggcttt gccctggcgc gaaacatcaa aaagctgtcg tccggcagca
     4381 gaaagcgact ttgcctggcg gcagatagcg ataacgtgtt ccagttgcat acggtcgaag
     4441 agatcgatgt tttctgcctc tgcgcccagc aacgccgtaa gcgcggaggg gcggctctcc
     4501 tgccagttat agcgcagacg gtttatctca tcttccacca cgtccagagt gatgcgtccg
     4561 ctagtggcaa aggtggccat ccgcgtgacg ctggcagaaa gttcgcgaaa gttaccgcgc
     4621 catgttgcct ggggagatgt tgcaaaggcc agccaggcac gccgcgcttc ggtgttaaaa
     4681 cgcacgctgt caccagtgag tgtggcgtgg cgctccactt cataatccag gttcggttca
     4741 atatcttcct ggcgctggcg tagacccggc agggtgaagg tccagagatt gatccgcgcg
     4801 tacagatctt cgcggaattt gccttcggca accagctggc gcaaatcgcg caccgttccg
     4861 gcgataagct gaaaatcact gctcacctgg cgatcgctgc caaacgggta aaaggttttc
     4921 tcttcaatag ctttcagcag cattgcctgt tcgtctgcgc ccagttcgcc aatctcatca
     4981 agaaacaaca ttccgccgtt ggcgctgcgt aataatcctt cacgagattc ccgcgccccg
     5041 gtaaacgcgc ctttcacatg accaaacagc gtcgacatcg cggtatcgcc gcgcagggtg
     5101 gcgcagttca cttccacaaa cgcgccgcta aactgatgcc gtgcctgttt taactcgaag
     5161 atgcgtcgcg ccagaaatga cttacctgcg ccggtcgggc cgttaagcag aatcggcgcg
     5221 cgggatttaa tcgccacttt ttcgatctgc tcaatcatgc ggttgaagtg ggggttacgc
     5281 gtggcaatac cggatttgag gaaatcgagc gtttgctgcc gttcctcggc aaagcggctg
     5341 gcgatggcgt tataacggct taaatcgaga tcgataatcg tcacttcacc tgggccgcgc
     5401 ggctgttctt ttttgcgcgg agttgaagat tgtatcaacc gggcgggtag ataacgcgct
     5461 tccgccagca gaaaccagca aatctgcgcg acgtgggtgc cggtggtgat gtgaattaaa
     5521 tagtcttctt tttctggctg aaactcgtaa ccacgggcga aatcatgcag gcaggcgtaa
     5581 acctcttcga aatcccacgg gttatgcagt tcaatctcaa cgcccaccac ttctgtttct
     5641 ggcgaaacgc tggcgatatc gcgtttaagc gtttcaaata gcgagcgcga gcgggcatcg
     5701 tgcaataatt ccagtcgatc gatgaccaac gattcttgct ggtatataca gagtgacgga
     5761 cgccattttg accagcgctg actgccgcgt ccggcataat ccagcacggt accgacaaag
     5821 ccaaaagcca ctgttttacg catcttagat atccttataa aaggcgatat ttcattatcg
     5881 gtaaatcggc tttgcgggaa atctccgttt acggatataa ag
//
LOCUS       DAZSIO010000193         5899 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZSIO010000193 DAZSIO010000000
VERSION     DAZSIO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.196"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..411
                     /locus_tag="ACHX5T_003614"
                     /pseudo
     CDS             1..411
                     /locus_tag="ACHX5T_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="ACHX5T_003615"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="ACHX5T_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="ACHX5T_003616"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="ACHX5T_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HFR8468638.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
     gene            5722..>5899
                     /locus_tag="ACHX5T_003617"
     CDS             5722..>5899
                     /locus_tag="ACHX5T_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468639.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQD"
BASE COUNT         1445 a         1488 c         1409 g         1557 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tagggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tggtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggggg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc gagaaccacg gcagcctgac ggctgcaaat tgaaaactgc
     5641 ccctgttcgt cctggtgttg ccgcacgctc cgcagaatat gtatgctctg aggacctgtt
     5701 ttatttagga ctcatcctct tatgttgacc cgcaaaagta tagataccgt tctgctctct
     5761 gttggtgctg agaagctctc acaacgtgaa tgggactgga tgaagatgct caaacccatg
     5821 gacccaccac ctgcgatggt cgcggcctcc atcctggagc gccgtggcga cacagcagcc
     5881 cttacccggc tacaggata
//
LOCUS       DAZSIO010000194         5840 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZSIO010000194 DAZSIO010000000
VERSION     DAZSIO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.197"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            218..1126
                     /locus_tag="ACHX5T_003618"
     CDS             218..1126
                     /locus_tag="ACHX5T_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFR8468640.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1172..3163)
                     /gene="tkt"
                     /locus_tag="ACHX5T_003619"
     CDS             complement(1172..3163)
                     /gene="tkt"
                     /locus_tag="ACHX5T_003619"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR8468641.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3441..4199
                     /gene="loiP"
                     /locus_tag="ACHX5T_003620"
                     /gene_synonym="yggG"
     CDS             3441..4199
                     /gene="loiP"
                     /locus_tag="ACHX5T_003620"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR8468642.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4405..5325)
                     /gene="speB"
                     /locus_tag="ACHX5T_003621"
     CDS             complement(4405..5325)
                     /gene="speB"
                     /locus_tag="ACHX5T_003621"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR8468643.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5461..>5840)
                     /locus_tag="ACHX5T_003622"
     CDS             complement(5461..>5840)
                     /locus_tag="ACHX5T_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468644.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1405 a         1543 c         1527 g         1365 t
ORIGIN      
        1 actttgtatt tgttatgttc atcacaaaat caacattttg tttgttattg tatttgtgat
       61 gcatatctct atttatttat tgaatgttcg aaaacatact tttctaatgc aatgtttttt
      121 cgctataccc ctttatagat tctgaattct cacctgtcgg ctgttttctc ctcatctcat
      181 gtgacatatt cccatacttt actcaaggaa gattgtcatg aaagcacctg tttcattaac
      241 ctcatttcgc ccacaaaaat ctcttgctat cgccattggc gtgcttgccg tggttgtgct
      301 gccatttctc tcgtattaca ccgttaatga aggtgaacgc ggcattttgc tgcgctacgg
      361 aaaaatcgta aaagttgccg aaccggggct gggttttaag atccctttta tggaaagcgt
      421 ggagaaaatt tccacgcgta atcaggctgt ggtctatcag ggattacagg cctacagccg
      481 cgaccagcaa ccggcacaaa tgacagtatc agtcagcttc cacattaaac cttcagaagc
      541 aggcgcggtt tataccacct acaacactat tgaagcatta aaagatcgtc taattgtccg
      601 tcagttgcca actcagctgg agaacatttt cggccaatat accgccatca gcgccgtaca
      661 ggatcgtaca aaattggttc aggatctgca aaatgccatg cgcaaagccg ttgttgggcc
      721 agtggtaatt gacggggtcc agattgaaaa tattgatttt tctgacgctt atgaaaagtc
      781 gattgaaaac cgaatgaaag cagaagtcgc cattgccacg cgtaagcaaa atctggaaac
      841 agagaaaatc caggcgcaaa tagcggtaac tcaggcccag gcagaagcag acagtaaact
      901 ggcggcagcc aaagcagaag cggaaactat tcgcgtaaga ggcgcggcag aggctgaaac
      961 tattcgttta aaaagtgcgg ctgaagcaga agcaatccgc ctgcgcggtg aagctctgcg
     1021 ggataatccc ggacttgtag ccctcaccac tgccgaacgt tgggacggta aactgcccga
     1081 taccatgatc cccggcagca cagttccgtt tatctctact aagtaaaaaa agggccgctt
     1141 tcgcggtcct tctcacaaaa cgtcttactg attacagcag cgcttttgct ttcgcaacaa
     1201 cgttatcaac agtgaagccg aactcttcaa acagcagctc tgccggagca gattcaccga
     1261 aggtggtcat gccgacgata gcgccgttca ggccaacata cttgtaccag tagtcagcaa
     1321 tacccgcttc taccgcaacg cgtgcagtaa ccgctttcgg cagtacggat tcacggtaag
     1381 ccgcatcctg cttgtcaaat gcgtcggtag acggcatgga caccacgcgc gctttcacgc
     1441 cttcggcagt cagtttttcg taggcagcaa ccgccagttc aacttcagaa ccggtggcga
     1501 tgaagatcag ttccggctga ccagcacagt ctttcagcac ataaccaccg cgcgcgatgt
     1561 ttgccagctg ctcttcagta cgttcctgct gcgccaggtt ctgacgggag aggataagcg
     1621 cagtcgggcc gtcctgacgc tcaacgccgt atttccacgc gaccgcggat tcaacctggt
     1681 cacacggacg ccatgtagac atgttcgggg tcacgcgcag agaagcgacc tgctctaccg
     1741 gctggtgagt cgggccatct tcgcccagac cgatggagtc gtgggtgtaa accatcacct
     1801 ggcgctgttt catcagcgca gccatacgta cggcgttacg tgcgtattcc acgaacatca
     1861 ggaaggtgga ggtgtacggc aggaagccac cgtgcaggga gataccgtta gcaatcgcgg
     1921 tcataccgaa ctcgcgaaca ccgtagtgga tgtagttacc tgcagcatct tcgttgattg
     1981 ctttagaacc agaccacagg gtcaggttag acggtgccag gtcagcagag ccgccgagga
     2041 attcaggcaa cagcgggccg aacgcttcga tagcattctg cgacgcttta cggctggcga
     2101 ttttcgccgg attagcctgc agtttagcga taaactcttt cgctttggcg tcgaagtcag
     2161 acggcatttc gcctttcata cggcgggtaa attcagccgc ttcctgcgga taagctttcg
     2221 cgtaagccgc aaacttctca ttccatgcag attctttcgc ctggcctgct tctttcgcat
     2281 cccactgagc atagatttca gacgggattt cgaacggtgc atatttccag cccagctgtt
     2341 cgcgggtcag ggcaatttca gcgtcgccca gcggcgcacc gtgggagtcg tgagtaccgg
     2401 ctttgttcgg ggaaccgaaa ccgatgatgg ttttgcacat cagcagggac ggtttgtcag
     2461 tcactgcgcg cgcttcttca actgcgcgtt tgatggatgc cgcgtcatga ccgtcgatgt
     2521 cgcgaataac gtgccagccg taagcttcga aacgcattgc tgtgtcgtcg gtgaaccagc
     2581 cttcaacgtg accatcgata gaaataccgt tgtcatcgta gaacgcaatc agtttaccca
     2641 gcttcagcgt acccgccaga gagcaaactt cgtgggagat gccttccatc atgcagccgt
     2701 cgcccatgaa ggcgtaggtg tagtggtcga caatgtcgtg gcccggacgg ttaaactgcg
     2761 ccgccagcgt tttttctgca atcgccatac cgactgcgtt ggcaataccc tgacccagcg
     2821 gaccggtggt ggtttccaca ccagcggtat aacctacttc cggatggccc ggagttttag
     2881 agtgcagctg acggaagttt ttcagttctt ccatcggcag atcgtaaccg gtgaggtgca
     2941 gcaggctgta gatcagcatg gagccgtggc cgttggacag cacgaagcgg tcacggtcag
     3001 cccaggacgg attctgcggg ttgtgtttca ggaaatcacg ccacaggact tcggcaatgt
     3061 cagccatacc cataggggca cccgggtgac cggatttggc tttctgtact gcgtccatgc
     3121 tcagcgcacg aatagcattg gcaagctctt tacgtgagga cattttgact ccagatcgga
     3181 tgatgaaggg cacgccctta acgacttgac gacagcgcgt tttgggctac gccggaaaat
     3241 ttgccaacaa tttaccgcaa gccgcgcgtc atgtacatgg aacatccttt tgccgcttca
     3301 gaaatctctg gatcatgctc gcatgttgcg caatctgctc gcccgtccgc tgcgcttttc
     3361 cttatactga gactgagcgt cgattcacct gcaaacggcg catttttaga ataatcctga
     3421 ccttgtgcgg aagagaaaac atgaaaattc gcgccttatt ggtagcaatg agcgtggcaa
     3481 cggtactgac cggttgccag aatatggact ccaacggact gctctcatca ggagcggaag
     3541 cttttcaggc ttacagtttg agtgatgcgc aggtgaaaac cctgagcgat caggcatgtc
     3601 aggagatgga cagcaaggcg acgattgcgc cagccaatag cgaatacgct aaacgtctga
     3661 caactattgc caatgcgcta ggcaacaata tcaacggtca gccggtgaat tacaaagtgt
     3721 atatggcgaa ggatgtgaac gcctttgcaa tggctaacgg ctgtatccgc gtctatagcg
     3781 ggctgatgga tatgatgaca gataacgaag tcgaagcggt gatcggtcac gagatggggc
     3841 acgtggcgtt aggccatgtg aagaaaggga tgcaggtggc gcttggtaca aatgccgtgc
     3901 gagtagctgc ggcctctgcg ggcgggattg tcggcagttt atctcaatca caacttggtg
     3961 atctgggcga gaaattagtc aattcgcaat tctcccagcg ccaggaagca gaagccgatg
     4021 attattctta cgatcttctg caccaacgcg gcatcagccc ggcaggtctt gccaccagct
     4081 ttgaaaaact ggcgaagctg gaagaaggtc gtcaaagctc aatgtttgac gaccatcctg
     4141 catccgccga acgtgcccag catattcgcg atcgcatcag cgcggatggg attaagtaaa
     4201 gcctagtggt gtcgaacgta ggtcagataa ggcgttcacg ccgcatctga cattaatggc
     4261 acatttgtta ccttgtgcgc attgccggat gcgatgctgg cgcatcttat ccggcctacg
     4321 ggtgccgaac gtaggtcgga taaggcgttc acgccgcatc cgacattaat ggcacgtttt
     4381 acccgtgcgc atcgcatctg gtgcttactc gccctttttc gccgcctgaa tatacagcat
     4441 ctccagcgcc agcgtcgccg cagccagagc ggtgatttcc gactgatcgt atgccggagc
     4501 cacttccact acgtccatcc caacgatgtt gagatctttc aggccgcgta ccagtttaat
     4561 ggcgcgatcg gaggtcaggc cgccaatcac tggcgtaccg gttcccggcg cgaaagcagg
     4621 atccaggcag tcgatatcaa aagtcaggta aaccggcata tcacccacaa tctgtttcac
     4681 ctgggcgata acgtcatcca cgctgcgatc gttcacctgg caggcgtcca gcacggtaaa
     4741 gccgttgtct ttatcaaact cggtacgaat accaatctgc acggaatgat tcgggtcgat
     4801 cagaccttct ttcggcgcgg tatagaacat agtgccgtgg tcaaattcac aaccgttcgc
     4861 ataggtatcg gtgtgggcgt caaagtgtac cagcgccatt ttgccgaaat gcttcgcatg
     4921 agcacgcagc agcggcagcg taacaaagtg gtcaccaccg aaggagagca tacgcttacc
     4981 ggcagccagc agcttctcgg cgtgcgcctg cagcttttcg ctcatctcac gggcatcgcc
     5041 aaaggcatat accagatcgc cgcagtccac gacgttcaga cgctcacgca tgtcgaaatt
     5101 ccacgggaag cggttgtgtt cccaggccag attcgtcgaa acctgacgga tcgctgccgg
     5161 accgtggcga ccacccgcac gaccagaagt ggccatatcg aacggcacgc cagtaatcac
     5221 ccagtctgca tcgctgtcat acggctggaa gttcatcggc aggcgtaaaa aaccaaaggc
     5281 attggaaacc agtgagttat cgtattgatg acctaaggtg ctcatggatg gactcctatt
     5341 acaaagtcga tataaaaaaa cccttccgcg tcgttaggcc cgacgaggaa gggttggatt
     5401 tgcgaaacac aagtttgtgc taattatcgc cttaaatggg cgaggcatca agcgaggttg
     5461 ctatttccga cacggtggcg gcgcatagcc attgttatct gccggaaagg cttgtccaga
     5521 gcacggcggc agcgcgtttt gcgccgggcc ttgtaattca aaggcggcaa agccgattac
     5581 cccggtatta cgcacatcgc cttgcgccga attggctgcg taggaatcag caacgttggc
     5641 gaactgaaaa gccgcttcag tatgtttatc tttgcggaat cctttaatca ctaacgaatc
     5701 tccggcattc acaatatagc cgttgttatt cagcgaacct tgcttgccgt tcagcacatc
     5761 caggccatca gccgtagcaa cgatctcgta gttggtgtta cgactgtaat tacggacata
     5821 taattgataa ttcattccga
//
LOCUS       DAZSIO010000195         5744 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZSIO010000195 DAZSIO010000000
VERSION     DAZSIO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.198"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..173
                     /locus_tag="ACHX5T_003623"
                     /pseudo
     CDS             1..173
                     /locus_tag="ACHX5T_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(489..827)
                     /locus_tag="ACHX5T_003624"
     CDS             complement(489..827)
                     /locus_tag="ACHX5T_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468645.1"
                     /translation="MVAETMFPNIAGSRSCIDCHHVAGRTFSKSARRIRGAPLLRRTS
                     RYDAQTSAFGISNDFRCISYVIPFWVVSSAMNVIPIPSLQTHYRSFITTTNGSAPVSR
                     YVPETGSHVP"
     gene            749..1876
                     /gene="ltrA"
                     /locus_tag="ACHX5T_003625"
     CDS             749..1876
                     /gene="ltrA"
                     /locus_tag="ACHX5T_003625"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR8468646.1"
                     /translation="MFDQQRDGNLYKIWNRLCSGTWFPPPVLEKRIPKSNGKERILGI
                     PTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDALKQCAIRCWRYSWILEVD
                     ISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSCENGELITRTRGTPQGGV
                     ISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRMSDATRLKNRLSERFSEV
                     GLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNFNGERYRKCMPGASNAAM
                     RKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFNYRLWSAMQSRL
                     LKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE"
     gene            1959..2138
                     /locus_tag="ACHX5T_003626"
                     /pseudo
     CDS             1959..2138
                     /locus_tag="ACHX5T_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2165..2650)
                     /locus_tag="ACHX5T_003627"
     CDS             complement(2165..2650)
                     /locus_tag="ACHX5T_003627"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR8468647.1"
                     /translation="MSEQKITGIDLAKTNFYLFSINAHGKPAGKTKLSRNQLLNWLVQ
                     QPKMTVAMEAGGASHYWAREIRKLDHDVILLPAQHVKAYQRCQKNDYNDAQAIAEACQ
                     HGTIRPVPIKTLEQQDVQTFLNMRRLVSMERTQLINHIRGLLAEYGIVFSKGAAELRQ
                     K"
     gene            complement(2906..3886)
                     /locus_tag="ACHX5T_003628"
     CDS             complement(2906..3886)
                     /locus_tag="ACHX5T_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFR8468648.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3886..5085)
                     /locus_tag="ACHX5T_003629"
     CDS             complement(3886..5085)
                     /locus_tag="ACHX5T_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR8468649.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5259..5393)
                     /locus_tag="ACHX5T_003630"
                     /pseudo
     CDS             complement(5259..5393)
                     /locus_tag="ACHX5T_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5407..>5744)
                     /locus_tag="ACHX5T_003631"
     CDS             complement(5407..>5744)
                     /locus_tag="ACHX5T_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR8468650.1"
                     /translation="HWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTS
                     DDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIG
                     SFIEKHMFY"
BASE COUNT         1574 a         1317 c         1368 g         1485 t
ORIGIN      
        1 ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta
       61 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
      121 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa attgcagcat
      181 aagttatcca tgctctgagg tgggtagagg tgggccctcc ggtcagggtt tacaggagca
      241 cagaccaaac gacccggtgc tgcttcattc taaggaatga ggtggtgagc gactcgggaa
      301 aaggcagaga tctactgtca cgtgggttcg ggaagctgaa cgaaagtaaa ctgtccgatg
      361 aagcatcgaa atcttagatg atgccaaaat ggggccgtac agccgtcccc tgataagcgc
      421 agaagacacc tgcttactga ctgcgcggca tccggtgtga aggcagcagg atcccgggcc
      481 agaccatctc agggaacgtg agaacctgtt tccggcacgt atcgggaaac aggggcggac
      541 ccattcgtag tagtgatgaa agatctgtaa tgggtctgga gcgaagggat ggggatcaca
      601 ttcattgcag aagacacaac ccaaaagggg atgacgtaac tgatgcaacg aaaatcattt
      661 gaaataccaa aagcactggt ctgggcatca tatctggatg tgcgtcgcaa caagggagcc
      721 ccccggatgc gacgggcaga ctttgaaaat gttcgaccag caacgtgatg gcaatctata
      781 caagatctgg aaccggctat gttcgggaac atggtttccg ccaccagtgc tggaaaaacg
      841 gatcccgaag tcgaatggca aggaacgcat tctggggatc ccaacagtat ccgatcgaat
      901 tgctcaggga gcgataaaac ttttcatgga agaaaagctt gacccgattt tccatgctga
      961 ttcatatggt taccgaccag gcaaatcagc tcatgacgct ctgaaacaat gtgccatccg
     1021 gtgctggcgt tatagctgga tactggaagt ggacatcagt gcgtttttcg accatgtgag
     1081 acatgatctg gtgctcaaag ctctggaaca ccacgggatg cctaaatggg tcatcctgta
     1141 ctgtcgacgc tggatggaag cgccaatgca gagttgtgaa aatggagaat taataacccg
     1201 aacacgaggc actccgcagg gcggggtcat tagtccgcta cttgctaact tgttccttca
     1261 ctatgcattt gatttgtgga tggaaagaga atatcggggg gtaccgtttg agaggtacgc
     1321 tgacgatatt gtagtacatt gttcacgaat gagtgatgca acaaggctaa agaacaggtt
     1381 atcggagcgc ttctcggaag tcgggctggt cctgaatgca gggaaaacga atatcgccta
     1441 cattgacacg tttaaaagga gaaacgtcgc aacgagtttc acctttctcg gatatgactt
     1501 caaagtgcgt acgctgaaga atttcaacgg cgaacggtac cgaaaatgca tgccgggtgc
     1561 gtcaaatgca gcaatgcgca aaataacaga aacaatcaag aagtggcgta tacatcgctc
     1621 aacagctgag agtttgctgg attttgcgag gcgctacaat gcgatagtga gaggctggat
     1681 cgagtactac ggaaagttct ggtccagaaa tttcaactat cgactgtgga gtgcaatgca
     1741 gtcacgtctg ctcaagtgga tgcagtctaa atacagactt tcgaaccgga aggctcagcg
     1801 aaagctgacg ctggtaagga aggagtatcc gaagctattt gttcactggt atttactacg
     1861 tgcatcaaat gagtgatcaa gagccgtatg acgcgagagt gtcacgtacg gttccgtgag
     1921 cagccgggga gtgaaattct ctccggctga ctcgactcgc gggttccgac agcgatgacg
     1981 aacatgcggc ggtgttgtac tcgctgatcg gcacatgccg tctgaacaat gtggaaccag
     2041 aaaaatggct gcgttacgtc attgagcata tccaggactg gccggcaaac cgggtacgtg
     2101 atctgttgcc ctggaaagtt gatctgagct ctcagtaaat atcaatacgg ttctgacgag
     2161 ccgcttactt ttggcgtaat tctgctgcgc ctttactgaa cacaatgccg tattcagcaa
     2221 gaagtccacg aatgtgattt atcagttgtg tcctttccat tgaaaccaac cgacgcatat
     2281 tcaaaaaagt ctggacgtcc tgttgctcca gagtttttat tggtacgggt cgtatagttc
     2341 catgttggca ggcctcagct attgcctgtg cgtcgttata atcatttttc tggcaccgct
     2401 gataagcttt aacatgttgt gccggaagca atattacatc atggtcgagt ttcctgattt
     2461 cccgggccca gtaatgagat gccccgccag cctccatcgc aacagtcatt ttaggctgtt
     2521 gcaccagcca gttaagcagt tgattgcgag aaagtttggt ctttcctgcg ggtttgccat
     2581 gagcattgat actgaaaaga taaaagttcg ttttagccag atcaatgccg gtgattttct
     2641 gttcagacat ggtatcccgc ttacttttgt tgttaaagtt tatccatcat ggcgcaattg
     2701 aaggctgccg tgaaagaggg ggaagactat ctcatcggtt actgttaaac ggctaagaga
     2761 aactatcttt ttcaagcaaa gaagatacgg tgaaacaggc gatttcaccg tgaaatactg
     2821 ttcaaaaaaa acgctaataa aagcaaaatt tcttttaaaa cagccaatta aaaactggcg
     2881 agttgctggc gaaaaacttg aattttcaca gcgaattcag attatctttt agtacaagag
     2941 caatcatacg atctaactta tcctgaactt ccaacggtaa tctgccgaat tcatatgaga
     3001 acgtacgtcc ctttactcgt tttcgagcaa atttatcttt actatcaaat ttccataaca
     3061 aagttacaac cgctttatcc tttactcctg aatcaagcaa tgaagacatt tccttggtta
     3121 taagccgcat aatactgttt ttaacctcgt cttcagaaag attgtcatca gcagtaataa
     3181 gctcaatact cggcgagata ttcttcacaa cttcatcaat agatatatca cccagcctca
     3241 gtcgttcact taattcagcc agttgcctgt aatcggcgta tgtcagctca gactgaacag
     3301 gaaaaagaga cacaaaatct ttaggtacgg acgctgcttg gagggcacga gtcaccttag
     3361 ctgccgacaa tccctctctt tccgcaattt gcttctggtt cataccggcc tctttgagac
     3421 gtactaaacg caacccaatt tcacgaatat tatgttcaag tgacgtctgc aaatctttcg
     3481 ccagatgctg agcttcagaa acagtaagct catcatccgt tacaagtaca cgtaatccaa
     3541 ccttgcatag caaagctgct gcccgacgac gggaaccatc caaaatctca ataagatcgc
     3601 cagtccgaat cccaatacat ggatagaatt gttgtaaacg tatagttctg gtgatgtctt
     3661 ttagtgagtc cggcgtcagg gctgtctgtt cgcggccatt cacctcttca accacaaaag
     3721 tttgagtatc cactttgtca tgatcaacct tgatctcagt aaattttgcc tttcggccag
     3781 tattgagggt aaatacatga actggagccg atatttcttc agtattattc aggatatttg
     3841 tattcagcgt acggccaata gtcggtcggt gctttctgtt ttccatcagt agttggccct
     3901 tataaattca atacgatcaa aaactgcttt agcgaaatct tctgctgcca tacgtgcatt
     3961 tttcaaagct tcaccactgc caacataagt aacaggattc gcagatatca cggtatcaaa
     4021 cgactcgccg cttctctcaa aaccatcaag acgtggcatt gaaacatcaa gcatgtcacc
     4081 accgaaaatc tcttttgtca ggctatggca gtatttatga tctgacttat tcgccagttt
     4141 cgacatgaag ccaatatttg cctgcaaacg gcatgaacat ccatcctgtt caataatctg
     4201 tacaagttca ggtaaacgag ccaaatattt aagcgtggaa tggaaatcaa cttgtgctgg
     4261 cggcaccggt gtaaacatga tatctgcggc agcaatggca tttttcaaaa atgcgtccag
     4321 atgcgggcct gtatcaataa ggatgaagtc gaaatcatgc ttcagcttat caataatatt
     4381 ctcacgcaat atcgcgtgtt tgttttgtcc taatagatgc tcctcgcaca gagtatccca
     4441 gtttgatgca ataaaggcgt cgtcaataga agccggcata acataaacgc cagggataac
     4501 tgacggaacg ataaaatctt ccagtaattc ctcacgactc acgttctgca acatagcctg
     4561 cggtgcggtt gtatcaacca gccccaccgc atgcaagtag ttcagaaaca tagtggctga
     4621 tgactgggga tctaaatcaa ggagcagaat acgtaaatct tctgacagca ggtgaggatg
     4681 agcacgcagg gcgtgagcta cggaaacact actcacggtt ttagacacac cgccttttag
     4741 actaccgatg aatatggaat atgcctcact ataacgatcg cgatatttag gaatgccacg
     4801 atgtgcatag atatcgataa tgttctggat ggttaatgca tattttgtcg cggttcctgc
     4861 tgggcgtttt tcaaactgat agccctgagc ctccatctca ccgactgcac catccacgct
     4921 tgcgcgcgtt aattttggaa gcttagctac agcagacttt gaatagactt gataaaaaac
     4981 atcagcctga aactcctgct tttgctcctg aatctgtttt gttagggcga gcaacatttt
     5041 atctgcacgc tgcgcaacct tacttaactg ctcaaaacta gtcattcccc ctccgtgcta
     5101 tgtaggattt taacttatta caaaaaaaac tgcattagca gggtaatgca tgcaagtaca
     5161 tattgtacat gcttatatta aaaatgcgcg cattgctgat ttattaatca tcggaatgaa
     5221 tattactgta agatagtatg cctttccggg agaaagacgc tgatttcccc cgaataatcc
     5281 agttcaccac cacactccgg acaacagctt tcttcaggct ccagatggtg tatttcgcgg
     5341 gaaacatgtt ccggtaacgg acggcgatga cgtgactgtc gcaacaggcg ggggtagtgc
     5401 ttccaatcag tagaacatgt gcttttcaat gaaggagccg atgacttttt cgtggatttc
     5461 cacggaacgc gagaagcaga ttgttttacg tgccagccgc ttgatgcggg tgcggagggt
     5521 ccggttgtta cgtgcaatac gttgcgtgaa gattttgccg gtcaggtgtt tctccttcgg
     5581 cacttctctg gcgtagctgc cccagtcatc gctggtcacc atgccgatac agaacggagt
     5641 caacagggct aacagttccc gacaggtctc atcattccgt ggcccgaaag tgtaagccaa
     5701 cacaccgcca gttttagtgt tgtacgcata ccagagccag tgtt
//
LOCUS       DAZSIO010000196         5738 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZSIO010000196 DAZSIO010000000
VERSION     DAZSIO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.199"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..241
                     /locus_tag="ACHX5T_003632"
     CDS             <1..241
                     /locus_tag="ACHX5T_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468651.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="ACHX5T_003633"
     CDS             complement(238..1179)
                     /locus_tag="ACHX5T_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HFR8468652.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="ACHX5T_003634"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="ACHX5T_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="ACHX5T_003635"
     CDS             complement(1684..2904)
                     /locus_tag="ACHX5T_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR8468653.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="ACHX5T_003636"
     CDS             complement(2908..3651)
                     /locus_tag="ACHX5T_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR8468654.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="ACHX5T_003637"
     CDS             complement(3542..5008)
                     /locus_tag="ACHX5T_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HFR8468655.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5738)
                     /locus_tag="ACHX5T_003638"
                     /pseudo
     CDS             complement(5008..5738)
                     /locus_tag="ACHX5T_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
BASE COUNT         1226 a         1618 c         1435 g         1459 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatgat
//
LOCUS       DAZSIO010000197         5716 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZSIO010000197 DAZSIO010000000
VERSION     DAZSIO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.200"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..297
                     /locus_tag="ACHX5T_003639"
     CDS             <1..297
                     /locus_tag="ACHX5T_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFR8468656.1"
                     /translation="KKNKQRKEQKPFLIPLLNPKAYLFFAALIPTFIDNNTNITLNFF
                     ILGVLFIFISFLTDLIYIAISLTIRDKLTPSFSRYISICSSIFILGTGIYFIFT"
     gene            complement(355..1071)
                     /locus_tag="ACHX5T_003640"
     CDS             complement(355..1071)
                     /locus_tag="ACHX5T_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468657.1"
                     /translation="MQLDEHSRKIAALVAYNDKQIKEMNRLITLLNERVNDLNTFTST
                     LEREGKVVSGISYSFRDSVSNVKFYINKGMDEASCKLQKSVSDTLTSLLREKLSFTLN
                     DVKKEWVDFEEQTSKEIKESLKQTTREAEVATGIIRKTMEKYTVTRIVEKVIVLIVLF
                     LLLMIAPVFYAEYLKMNISSLMEEQKKHESMLKEWKEKAPLAEFSKCGDYRKACVLVD
                     DESGYYFNEQSPNKKYAILK"
     gene            complement(1092..2177)
                     /locus_tag="ACHX5T_003641"
     CDS             complement(1092..2177)
                     /locus_tag="ACHX5T_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFR8468658.1"
                     /translation="MASYHCTVKTGAKGVALKHADYISRSGEYKNYKSREDLEFSSSG
                     NMPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKK
                     ARFGETPQERKSALVALRSHWADVQNEHLARHGHQSRVDHRSLKEQGIKRTPEVHLGP
                     VRVAVMNADQVCAILARRNAEREKLEAEQERDSIIDLKTTLKEALAERDNIPPSLDEL
                     GAELKAFVESSFDELNEVMQLNSLINGALDSMMKQFQVEEERKLQQERLIEAEKQRDL
                     ERERERVRQRAYSSPAPSRSRDYGMSR"
     gene            2349..2795
                     /locus_tag="ACHX5T_003642"
     CDS             2349..2795
                     /locus_tag="ACHX5T_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFR8468659.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKT
                     LSALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVD
                     SKTGKPKFSAAELVGALAGIAELPRNHPKWQEWEKRGKELLTKDSA"
     gene            complement(3002..3931)
                     /locus_tag="ACHX5T_003643"
     CDS             complement(3002..3931)
                     /locus_tag="ACHX5T_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR8468660.1"
                     /translation="MSAVLQLFREKLPHKPYCTNDFTYGVRILPKDIAILARFIQQNQ
                     PHAQHWLAFDVDRTGAAIDWSDRNCPAPNITVKNPRNGHAHLLYALTLPVRTAPDASA
                     SALKYAAVIERALCEKLGADVNYSGLICKNPFHQEWQVVEWRWELYTLDELADYLDLS
                     ASARRRVDKDYGMGRNWYLFEKGRKWSYRAIRQGWPAFSPWLDAVIQRVEMYNASLPV
                     PLSPAECRAIGKSIAKYTYRKFSPEGFSAVQAARGRKGGNANSSENQAIKGQKSKRVA
                     VATSARTLKPWVTLGISRATYYRKLKCDPDLAK"
     gene            4484..5434
                     /locus_tag="ACHX5T_003644"
     CDS             4484..5434
                     /locus_tag="ACHX5T_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468661.1"
                     /translation="MNNFINECISAPRINGMAEFFSSTASGEKHKPFRLVEIDDRLFH
                     SYDYIKFAKHLTKNSGPFVNHYFCSIPYSKEEECRLGTTVIKYAITLNKKLNTWCLGM
                     AEGAMARTISELSKGNIQTLTTSPTKENEHAFFKYGSSENAYFICTPFFLLEERLKNS
                     NLKQFNQGFDIIIEDTTFQMYSPNRSGQINHVKRLLNNDGIFIFTEKHSSEHDEYQKR
                     EIQKDYSFKQRYFSKDEINTKKEIVLNTMNLNEVSVDDMLSAIKQHFKYASIYWNSGN
                     FYSIAASNSLKHISTFINLLPKAAIPMEYVYEKLPRNISW"
     gene            5441..>5716
                     /locus_tag="ACHX5T_003645"
     CDS             5441..>5716
                     /locus_tag="ACHX5T_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFR8468662.1"
                     /translation="MEYSTLLSFAIVTLSQTISIGPGVALVINNAFSHGLKSSIKTSI
                     YIRIGETIVMAISLFALSSTSSTEQHFHIIKIFGGGYLIYIGLMGLIN"
BASE COUNT         1713 a         1311 c         1108 g         1584 t
ORIGIN      
        1 aaaaaaaaca aacaaagaaa agagcaaaaa ccttttttga taccattatt aaaccccaag
       61 gcgtatcttt tctttgctgc gttaatacca acctttatag ataataatac aaatatcaca
      121 ttaaactttt ttatattggg tgttttattt atatttatat cattcctaac agatcttatt
      181 tacatagcta ttagcttaac gataagggat aaattgacgc catcattctc aagatacata
      241 agcatatgta gttccatttt tatattaggt acaggtattt attttatttt tacgtgaaac
      301 gccgttttta tactcatgca gccttagtca taagacggca tgagttactt aacactattt
      361 cagtatggca tattttttat ttggtgattg ttcattaaaa taatatccgc tttcgtcatc
      421 aactaacacg catgctttac gataatctcc acacttcgag aactcggcca atggagcttt
      481 ttctttccat tcttttaaca tggattcatg ttttttctgt tcttccatta gcgaactaat
      541 attcattttg agatattcag catagaaaac aggagcgatc attagcaata aaaataatac
      601 aatcaacaca attacctttt cgacaatcct tgttactgta tacttctcca ttgtttttct
      661 aattatacct gtcgcaactt cggcctctct ggtcgtctgc tttaatgatt cctttatctc
      721 tttacttgtt tgctcctcga aatcaaccca ctctttcttt acatcattga gagtgaaaga
      781 taatttttcg cggagtaatg acgttagtgt atctgagact gatttctgta atttacaaga
      841 tgcctcatcc attcctttat taatataaaa tttaacatta gacacactat ccctaaagga
      901 ataagaaatt ccagaaacaa ctttaccttc acgttcaaga gtacttgtaa aggtatttaa
      961 gtcattcact ctttcattaa gcagagtaat tagcctgttc atttctttta tttgtttatc
     1021 attatacgca acaagagcag caatttttct tgaatgttca tccaattgca taaaataaaa
     1081 acctccagtg cttaacggct cataccataa tcacgtgaac gtgatggtgc tggtgaggaa
     1141 tacgcacgct ggcgcactct ttctctttcc ctctccaggt ctctttgctt ttcggcttca
     1201 attagtcgtt cctgttgcaa cttccgctcc tcttccacct ggaattgctt catcattgag
     1261 tccagcgcac cgttaatcaa tgaattaagc tgcattacct cattgagttc atcaaacgag
     1321 ctttcaacaa acgccttcaa ttctgcccca agctcatcca ggcttggtgg tatgttatcc
     1381 cgttcagcta acgcttcctt caaggtcgtt ttaaggtcaa tgatgctgtc ccgttcctgc
     1441 tctgcttcta gcttctcccg ttccgcgttt ctccgtgcca gaattgcaca aacctgatca
     1501 gcattcataa cagcaacacg aacgggacca agatgaactt ccggtgtgcg cttgataccc
     1561 tgttctttca gtgaacgatg atcaacgcgc gactgatggc cgtgccgcgc aaggtgttca
     1621 ttctgcacat cagcccagtg agaacgtagc gcaaccaagg ctgattttcg ctcttgtggt
     1681 gtctcaccaa agcgtgcctt ttttgcacct ccccgctccg gtgatttgct gttggcacgt
     1741 ttaaaaaact gctgcggatc gcgttcaata ccatcattaa tccgctcgca aaacatgata
     1801 tgtgcgtgtg gttgttcctt tccgtcgatt gcacctggcg gattgtggat cgcgtactga
     1861 taggcatggc gatcaccaag ctccttttgc acgaaatctt caacaagttc tatccgttgt
     1921 tcgcgtgtca gttctcgcgg taatgcgatc tcaatttcac ggtatgccgt cccgtttttt
     1981 cgttcaaatt catctgctgc tttccatagc tctgcgggat ttttcttcgc ccatgatggc
     2041 atgttaccgg atgacgaaaa ttccagatcc tcccgcgatt tgtagttctt atattcgccg
     2101 gatctagaaa tataatcagc atgttttaag gctacgcctt ttgctccagt tttaactgta
     2161 caatggtagc ttgccatctg ccgttaagcc ctccgcagga gcgcactgtt tctcgacgaa
     2221 ggagagaaac gtctaagtgc gcatccctga attaatcagg gatgatttta aggtctcaca
     2281 cgcaggcaga caaggtgata ttatgcacaa taacaactca cattaacggc aaaagtgcag
     2341 aggtaagaat gacaaatcgt gaagagtggt taagtgcaaa aattgcctat atcaacggac
     2401 tgaaatcacc gtcagagcag caaagattac ttgtgttact ggcagagaaa aaaaaccgaa
     2461 caacgacgga tgaaaaaacg ttgtccgcac tgatacgggc agaaaaaacc gcagagaaag
     2521 cagccgcagc aaaagccagg gtaacggcaa taatagcggc agaacggaag gcagccgcaa
     2581 gggcggaacg aaaggcgcgg gatcatgaac tctacaaggc cgcaggcctc atgattgtgg
     2641 caggactggt ggacagcaaa accggaaaac caaagtttag tgctgccgaa ctggtaggcg
     2701 cactcgcagg aatcgcagag ctaccgcgca accatccaaa atggcaggaa tgggaaaaac
     2761 gcgggaaaga gctactcacc aaagacagtg cgtaagagcc tccgttcacg ttccggttcc
     2821 ctggtgcttc ctcgctcatt cgctcgctac gctcggtcat tgagactgcg gcgagcacaa
     2881 ccaccactca cacacagacg ctaaaaccat tgccaaacga aacacgggaa atcaccccaa
     2941 acctacccgc tcaaaaaaac gcctacaggc gctgttatct gataaggctt gttatctggt
     3001 ctcattttgc gagatctgga tcacatttta gttttcgata gtatgtggca cgactgatcc
     3061 ctaacgtcac ccacggcttc agagttcgtg ccgatgtggc aacggctaca cgctttgatt
     3121 tctgaccttt aatagcctga ttttctgatg aatttgcatt accgccctta cgacctcgag
     3181 cagcctgtac cgcggaaaag ccttcaggag agaatttgcg gtaggtgtat ttcgcaatac
     3241 tcttgccaat agcccgacat tcagccggag aaagcggaac gggaagcgat gcgttgtaca
     3301 tttcgacacg ctgaatcact gcatcaagcc acggtgagaa tgctggccag ccctgacgaa
     3361 tcgccctgta agaccattta cggccctttt cgaacagata ccagtttcgc cccatcccgt
     3421 aatctttatc gacgcgacgg cgcgctgagg cgctcaaatc aagataatcc gccagttcgt
     3481 cgagggtata gagttcccaa cgccattcca ccacctgcca ttcctggtgg aacggatttt
     3541 tgcagatcag gccgctgtag ttcacatccg cgcccagttt ttcacacagc gcacgctcaa
     3601 tcacggcagc gtatttgagc gccgaagccg atgcatccgg cgcggttctc accggaagag
     3661 tgagcgcgta gagcagatgc gcgtgcccgt tacgtggatt ttttacggtg atgttcgggg
     3721 ccggacagtt ccggtcgctc cagtcgatcg ctgcccccgt ccggtcaacg tcaaaggcca
     3781 gccagtgctg cgcatgtggc tggttctgct ggatgaaacg ggcaaggatg gcgatatctt
     3841 tcggtagaat gcgaacgccg tacgtgaaat cgttcgtaca gtacggtttg tgcggtaatt
     3901 tttccctgaa gagttgaagt acggcgctca tgtgttagga tttcctccgt gtgttacgac
     3961 ccacacgccc cttgaatttt cgtgacagtc ttggcggatc ggcacgaatt tcaggggcgt
     4021 ttgtttttca gttggttaca ctacacccaa cggcaaatcc tgtcgatcct tcccgaaaac
     4081 cagacccatt taccataact cctcttgtac gtaccctcta aaacgctctc aatcgctttc
     4141 tgacgcaaaa aactcaaaaa gacaagataa acctagggaa aacacaaaaa acgcgtcaga
     4201 atcgttttta ggggtctgca taactatgca caaaagcgat taatgatcgg tttcacccct
     4261 tcagaagcgc gaactggtca aaacagcaaa ctttctcatc cagaaagcca gctgaactaa
     4321 cccgcatgaa aacaaatttc caaacatatc cagtctggac aacgaaatat cgacaatata
     4381 aaacatcata atcgtgttta tggcgtgtaa aaaagcttac aagagaatta gcattgaaga
     4441 gtcataacaa caaattccaa aaatcaaacc agaggatagg aaaatgaata attttataaa
     4501 tgaatgtatt tccgctccga gaataaacgg catggcagaa tttttctcat caactgcatc
     4561 aggggaaaaa cacaaacctt ttagactagt agaaatagat gatcggcttt ttcattctta
     4621 tgattacatt aagtttgcca aacacctcac aaaaaacagc ggccctttcg ttaatcatta
     4681 tttttgcagt ataccataca gcaaggaaga agagtgcaga ttaggaacaa cagtaataaa
     4741 atacgcaatc acgctaaaca aaaaattaaa cacatggtgt cttggtatgg ctgagggcgc
     4801 aatggcaaga accatttcag aattatcaaa aggaaacata caaacattaa caaccagtcc
     4861 aacaaaagaa aatgaacatg cattttttaa gtatggctca agtgaaaacg cttattttat
     4921 ttgcactcca tttttcctgt tagaggaaag actaaaaaat tcaaatctta aacaatttaa
     4981 tcaaggattc gatattataa tagaagacac cacatttcaa atgtactcac ctaacagatc
     5041 tgggcaaata aaccacgtaa aaaggctatt aaataatgat ggtattttca tatttacaga
     5101 aaaacatagt tcagaacatg atgaatatca aaaaagagaa atccaaaaag attattcatt
     5161 caaacaaagg tatttctcga aagatgagat taacaccaaa aaggaaatcg tactaaacac
     5221 aatgaatttg aatgaagtta gtgttgacga tatgctttcc gcaattaaac aacattttaa
     5281 atacgcatca atatattgga atagcggaaa tttttactca atcgccgcca gcaattcact
     5341 aaaacacata tcaacattta taaatttatt accgaaagct gcaattccta tggaatatgt
     5401 atatgaaaag ctcccacgaa atatatcatg gtaagtaaac atggaatatt caacattatt
     5461 atcatttgct atcgtaacct tatcgcaaac tatatcaata gggcctggcg ttgcattggt
     5521 tataaacaat gccttttcac atggattaaa gtcatcaata aaaacaagta tatacataag
     5581 aattggcgag actatcgtca tggcaatatc tcttttcgcg ttatcctcaa catccagcac
     5641 agaacaacat tttcacatta taaaaatatt cggtggaggc tatttaatat acataggatt
     5701 aatgggttta attaat
//
LOCUS       DAZSIO010000198         5702 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZSIO010000198 DAZSIO010000000
VERSION     DAZSIO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.201"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..170)
                     /locus_tag="ACHX5T_003646"
     CDS             complement(<1..170)
                     /locus_tag="ACHX5T_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468663.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="ACHX5T_003647"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="ACHX5T_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR8468664.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="ACHX5T_003648"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="ACHX5T_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /gene="ygbI"
                     /locus_tag="ACHX5T_003649"
     CDS             complement(3616..4383)
                     /gene="ygbI"
                     /locus_tag="ACHX5T_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR8468665.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5487
                     /gene="ltnD"
                     /locus_tag="ACHX5T_003650"
                     /pseudo
     CDS             4579..5487
                     /gene="ltnD"
                     /locus_tag="ACHX5T_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
     gene            5484..>5702
                     /locus_tag="ACHX5T_003651"
     CDS             5484..>5702
                     /locus_tag="ACHX5T_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HFR8468666.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
BASE COUNT         1410 a         1469 c         1481 g         1342 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
     5401 gccagtaacg cgggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
     5461 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
     5521 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
     5581 gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc
     5641 gctcctgtcc ggtggttgaa gccacacagc aatcgctggc ggctctgagc tggttgcaac
     5701 ag
//
LOCUS       DAZSIO010000199         5631 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZSIO010000199 DAZSIO010000000
VERSION     DAZSIO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.202"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..706
                     /locus_tag="ACHX5T_003652"
     CDS             <1..706
                     /locus_tag="ACHX5T_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR8468667.1"
                     /translation="KKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFS
                     LESVFILVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVA
                     GVLSAAFYVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVF
                     SSGILQYFLAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFI
                     VTVLSVYFLNRKYSEE"
     gene            complement(1909..2100)
                     /locus_tag="ACHX5T_003653"
     CDS             complement(1909..2100)
                     /locus_tag="ACHX5T_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR8468668.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENR"
     gene            2165..2488
                     /locus_tag="ACHX5T_003654"
     CDS             2165..2488
                     /locus_tag="ACHX5T_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFR8468669.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     ARDDS"
     gene            2697..3128
                     /locus_tag="ACHX5T_003655"
                     /pseudo
     CDS             2697..3128
                     /locus_tag="ACHX5T_003655"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3166..3696
                     /locus_tag="ACHX5T_003656"
     CDS             3166..3696
                     /locus_tag="ACHX5T_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFR8468670.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSN
                     ESLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETN
                     WTKDNGKLNAVRLVQE"
     gene            3703..3936
                     /locus_tag="ACHX5T_003657"
     CDS             3703..3936
                     /locus_tag="ACHX5T_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFR8468671.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDN
                     AMLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
     gene            4417..5469
                     /locus_tag="ACHX5T_003658"
     CDS             4417..5469
                     /locus_tag="ACHX5T_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468672.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLR
                     KWVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARR
                     GEVSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKD
                     GLKIYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALA
                     DIRNMTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPG
                     ELTKDERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSF
                     QPRKFENKPLYEKEDD"
     gene            5483..>5631
                     /locus_tag="ACHX5T_003659"
     CDS             5483..>5631
                     /locus_tag="ACHX5T_003659"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFR8468673.1"
                     /translation="MLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVILSWI
                     VLFIK"
BASE COUNT         1484 a         1155 c         1515 g         1477 t
ORIGIN      
        1 aaaaaaactc ccattaaact caaaagcgat acttgcttat gcgacaggcg tttttgaacc
       61 attcttagcg tatactttta cactctacgg actttatttt atttcagcag gaagcgcatc
      121 aattattttt tcacttgaat cagtgtttat attggtgttg tctgttctta tttgctcagt
      181 taaaattaat tccccgcaat attttatatt gttccttata ggtgcaattg ttggcagtat
      241 tttggtagcc ttgccagaca tcagtaatgg atcgaataat attgttggat attgtcttgt
      301 tgttgccggt gtgttatcgg cagcatttta tgttgttata tcgtctcgcc ttattaaatc
      361 gtttgagcct atcacattat tgagcggaca gttgacattc tcccttatac tgtcatcgct
      421 ctttattttt atatctaaat catcaattta tcttccgttg gaatccgtga ttattgtttt
      481 ttcatctgga atattacaat attttttagc tttttgtttt tacttgaact ccttgcgttg
      541 gctcccagtt catttggcag ggataatgtt gtactttatt ccgctagttg cattattttt
      601 gtcatggttg tttttggatg aacaattcac attcttacag ggggtaggat ttattgtaac
      661 tgtgctaagt gtatattttc ttaacagaaa atactcagag gagtaacatt ctgttcagga
      721 ttgccccccg gcctccatcg ataccgtgcc aaatgcacgt aaaaaacggg agcgtttctg
      781 gtgtcggaat gtaaccaagg gggtcagagg cgcgctaaaa cgctctcagg ggagtcaaat
      841 agagacgttt gtaacaaaaa gatcaaagga tcctcttgag atcctttttt tctgcgcgta
      901 atctgctgcc tgtaaacgaa aaaaccgcct tgcagggcgg tttgatcgaa ggttaagtca
      961 gcttgcagac tgcttaacca ggtaactgtc ttatgcgaga cgagtcacca aaattgttct
     1021 tctagtgtag ccgcactccg gcagccactt caagtactct ctaaatatct cgcacatgac
     1081 ttttaccaat ggctgctgcc agtggcgctt tgtggtgtct tttccgggtt ggacccaaga
     1141 gaacagttac cggagaaggc gcagcagtcg ggctgaacgg ggggttcctg catacagccc
     1201 agcccggagc gaactgccta agtcgccccg agataccaga agacgtgagc tatgagaaag
     1261 cgccacgtgt cccgtaaggg agaaaggcgg aaaaggtatc cggtaatcga tatggcggga
     1321 acgtgggggc gcaggaggga gcgattcacc ggaaacggtg atgatcttta agccacgtcg
     1381 ggtttcgcca ccgctggcgg atttcatggt tgagccgcaa ggctcccaca gatgcaccga
     1441 aaaagtgtct gttttgtgaa gtccgtcagg ggggcggagc ctatggaaaa agcgcctccg
     1501 gcgcggcctt tcccggggtt tcgttttttg cgcacatgtt ctttcctggt ttatccccgg
     1561 gttctgtgga taacagggtt agcgtttttg agaaaggaga gtccgctcgc cgcagccgaa
     1621 cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga gcgcctgaac tggcattttc
     1681 tccttacgca tctgtgcggc atttcacacc ggacatggcg cacttttcat accatccgca
     1741 ctgatgccga atggttaagc cagtatacac tccgctatcg ctacgtgact gggtcagggc
     1801 ttcgccccga caccccaaag gtcaacggcc tgacgcgccc tggcgggctt gtctgctccc
     1861 ggcatccgct cacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agcggttttc
     1921 accgtcatcc ccgaagcgtg cgaggcagct gcggtaaagc tcatcggcgt ggtcgtgaag
     1981 cgattcacag atgtctgcct gttcatccgc gtccagttcg ttgagcttct ccagcagcgt
     2041 taatgtctgg cctctgatga agcgggccat gttaagggcg gttttttcct gtttggtcac
     2101 tgatgcctcc gtgtaagggg gggttctgtt catgggggta atgataccga tgaaacgcga
     2161 gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgctgtga
     2221 gggtaaacaa ctggcggtat ggatgcggcg ggtctgcctg ggtgagccgg ttgcccgttc
     2281 cgggaagctg ccgacactgg caccgccgtt actgcgtcag ctggccgcca tcgggaataa
     2341 ccttaaccag acagcgagga aggtgaacag cgggcagtgg tcttccggtg accgggttca
     2401 ggtggtggcc gcactgatgg ccatcgggga tgagctgcgc cggctgcgtc tggcggtcag
     2461 ggaacagggg gcgcgggatg atagttaagt ttcatgccag gggaaaaggt ggtggcagtg
     2521 gtccggttga ttacctgctg ggaagggagc gtaaccggga aggggcaacg gtgcttcagg
     2581 ggaatccgga agaggtccgg gaactcatcg atgccacgcc atttgcgaag aaatacacgt
     2641 caggtgttct gtcgttcgcg gagaaggagc tgccgcctgg agggcgtgaa aaagtgatgg
     2701 cgagctttga gcgtgtactg atgcccggtc tcgagaaaaa tcagtacagc atcctgtggg
     2761 tggagcacca ggacaaggga cggcttgagc tgaattttgt cattccgaac atggagctac
     2821 agaccggaaa acgcctccag ccgtactacg accgggcaga caggccgaga attgatgcct
     2881 ggcagacgct ggtgaatcac cattacggtc tgcatgaccc caacgccccg gagaaccgcc
     2941 ggacgctgac ccttcctgat aacctgccgg aaacgaaaca ggcgcttgct gagggcgtca
     3001 cacgcgggat agacgcgctt taccatgccg gagagataaa aggccgtcag gatgtgattc
     3061 aggcgctcac agaggcgggg cttgaagtgg tcagggtgac gcgaagcagt atcagcattg
     3121 cagacccgaa cggcgggagg aatatcagac tgaaaggagc gttttatgag caatctttta
     3181 cagacgggcg cggagttcga gaaaaagctg aaagagagag ccgaatctac agagaaaatg
     3241 ctgaacgacg agttcaggaa gctcgaagaa tctgtaaaca gggaactgac ctcaaacgaa
     3301 tctttaatca ggaacgctat caacgaccat acaacagccc tgaaagaact gctggagagg
     3361 tatcagaaaa cgaccgtcga tacgatggac gcgcactgga aaaccgtact gaaaatgagc
     3421 gtgaaacgct ggctgtggct gattatcgtc tcagtactga tgtttgccac gaccgggagt
     3481 ctgctgtggt accagggcat gaagataaac gcgaatatga atattctccg cgagcagaaa
     3541 gagagtctgg agaagctgaa cgcgaagaca tggggcgtgc gttatcacga ggacagcaac
     3601 ggacgttttc tggtgttgcc gaaggggatg aaagccgaaa cgaactggac gaaggacaac
     3661 ggcaaactga acgcagtgag actggtgcag gagtgacaga gcatgacgga gctggaaaga
     3721 catttgctga acgcattaga gcaactgcaa caggactata tgcagcggct gaacgaatgg
     3781 gagaacgcct tcgtggaatt gcagaaaatg ttttcgctta cgcaacggga caacgcgatg
     3841 ctgaacgggc gagtcatgca gttgagtcag caggtgcagc acttgagcga gcggacagaa
     3901 cgcttgagcc agttatacag cgagaacagg agataaggga tgaacggctg atgcaggaac
     3961 gcgaacatga gttatctctg gagcgggagc gtcagccgga aatccaggag cggacactgg
     4021 atggcccttc gctgggatgg tgaaaccatg aaaaatgacg tgttcagtgg attaaggcgg
     4081 gctaatgtgg cctgtagcat ctggttgcat aggtattcat gcggttaaaa tttatcaggt
     4141 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     4201 gcaccgaacg cgttttggcg gtgcgtacaa tgcgacgtta tggtaaatgg agcggatttt
     4261 caggaaggag tgatactaac aactcgaaca ctgtgcaaat aatgattttt ttaaaatatc
     4321 ggtaacgata ataaattatt tcgttttacg ccggtaataa atataatgta gtttcagtat
     4381 ataaaaaaat atatgaggtg tcttgtggga gctttcatga atcttcaaca acgtataaat
     4441 aaactaccac aattatcatc cagtttttca tttggaaaag atattgataa tattcattca
     4501 tttatcttta acgaaacttc taaagataaa attgaagatt tactaagaaa atgggtgtca
     4561 ggcaatcagc cctgtgtatt tggtaaattg gcaagcaaga aaattaaagg gcttgatttc
     4621 catttatcta ttgtaaatag cccccaacta tataatgatg atggtcattt gtttgatttt
     4681 ttgcgtaatg aaagagttcg ttttaaagaa cgcgcaagaa gaggagaggt cagtgcgcat
     4741 ctcatttatt ttattcatcc acaacttgcc tttgctcgtc catcagaaga gttagtggac
     4801 atacaaaaat atatttgttc gctgcatatg ccagagtgct atccaataaa agaggatgtt
     4861 atttatactg aaagcgtacc atttcaggat aaagatgggc taaaaatata taaggctggt
     4921 gttaatgttt tttactcttc tgctcataga acaagaaatc atgatagaag aataccaggg
     4981 ggaatactta tttctgtaaa tgccccgggg cattttatga ggctggcgat agaaaaaggt
     5041 ttctataaag atcaagaaca agcgttagcg gatattcgta acatgactat tcagtctgtt
     5101 ggtaagggtg ggtattcaca tccggaaggg atttctacaa catggcattc agaatctaat
     5161 ttggatcgtt ttggatgccc agtgcacacc ggtaatagtt cttattacag cggtttttat
     5221 catactgatg tattaattcc aggagaatta actaaggatg aaagattgct ccatgaaata
     5281 gaaaatagtg atccaatgat cttcaactgg aatgtattgt tttatgtttc attagaagag
     5341 tttcctatag atgatcctta ttatggagag tttattggta ttcctgtaga tgatgcatct
     5401 atgtttttta actcctttca accaaggaaa tttgaaaaca aaccccttta tgagaaagag
     5461 gatgattaat atgattaaag gaatgctttt tgctttttta gcagcgttct catggggggc
     5521 agctatcgta atgagcaaaa aaggagttga agaactagat gccggagggg tgtttttctg
     5581 gcagatttgt agtgcagtaa tactatcttg gatagttctt tttataaaaa g
//
LOCUS       DAZSIO010000200         5592 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZSIO010000200 DAZSIO010000000
VERSION     DAZSIO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.203"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..694
                     /locus_tag="ACHX5T_003660"
     CDS             <1..694
                     /locus_tag="ACHX5T_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR8468674.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1033..1803)
                     /locus_tag="ACHX5T_003661"
     CDS             complement(1033..1803)
                     /locus_tag="ACHX5T_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468675.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1848..2334)
                     /locus_tag="ACHX5T_003662"
                     /pseudo
     CDS             complement(1848..2334)
                     /locus_tag="ACHX5T_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(2294..2515)
                     /locus_tag="ACHX5T_003663"
     CDS             complement(2294..2515)
                     /locus_tag="ACHX5T_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468676.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2516..3199)
                     /locus_tag="ACHX5T_003664"
     CDS             complement(2516..3199)
                     /locus_tag="ACHX5T_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR8468677.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3263..3574)
                     /locus_tag="ACHX5T_003665"
     CDS             complement(3263..3574)
                     /locus_tag="ACHX5T_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468678.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3571..4473)
                     /locus_tag="ACHX5T_003666"
     CDS             complement(3571..4473)
                     /locus_tag="ACHX5T_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFR8468679.1"
                     /translation="MYFPSEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4913..5592
                     /gene="parM"
                     /locus_tag="ACHX5T_003667"
                     /pseudo
     CDS             4913..5592
                     /gene="parM"
                     /locus_tag="ACHX5T_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
BASE COUNT         1420 a         1531 c         1419 g         1222 t
ORIGIN      
        1 tgatgcagaa gccatatgtg aagcggcttc acgaccatca atgcgttttg tccagccaag
       61 aactgagacc cagcaagcaa taagggcatt acaccgggtc agagaatcgc tcatcagaga
      121 taaggtgaaa acaaccaacc agatccacgg tttcctgctg gaatttggaa tcagcctgcc
      181 gacaggggat gccgtaataa aacggttgtc tctcgtattg gctgaacacg aaatccccga
      241 atatctgagc cgccttttgg tgaggttgca tacccactac ctttaccttg ttgagcaaat
      301 agcagagctg gaatcagaat taagtcagtc aatcaacgct gatgataccg ctcagcgcat
      361 tatgacaatt cctggtgttg ggccgattac tgccagctta ctatcgtcgc aacttggtga
      421 cgggaaacag ttttcatgca gcagagattt tgcggcttcc acagggctcg ttccccggca
      481 gtatagcact ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg ataaaaattt
      541 acgccggtta ctggttcagt gtgcccgctc attcatgatg cagcttgaac gccagcatgg
      601 aaaacttgct gagtgggttc gggagcaact caacaagaag cattcgaatg tggttgcctg
      661 cgctctggca aataaactgg cccgaattgc ctaggcaatc actactcagc aaaaagaata
      721 tcaggcctga tatatagatg ctgacaaaac tcaattagct tattgcaaac aataagatac
      781 aagttaccgt ctggttttgc gaagattgat tattgatgac atgaacggca aatcgacctg
      841 attaaaaacc taacagggga aaatggctca ctgaagccga ggggattttt aggattatca
      901 ggtgcggaac tcatcgaggc gcgggcatcc cttgcccaca gagacgccgg atagatttaa
      961 gcaagcccac cattaatcaa aaatcagtgt tgcaaaaacg ggggtgacca tagattttcc
     1021 ccgccatcgt catcatttcc ggatttttgc cagggcatcc gccatgctgc ggatttcggc
     1081 ccgggcttcc cggacaactt cccgcagaac accacggtca aaccaccggc ggggcggttc
     1141 atccggtata cgcagcacat cgcaggccag cccactggac aggatatgct caatacaatc
     1201 gctgaccaga tacatttcca ccgccgaaaa acgtgcgccc cggttcagga tggcatcgag
     1261 caccacgggc aggttttcat cgtcttcatc gagccagcag gggtgatcgc tttccttcac
     1321 gaccaggcga aagcggcgcg tctggcgggt cagcaacgtc tgaccgcttg tggtcagcgc
     1381 cgcccggata cgccgcgcat cttctgcccc ccggcaactg acactgcata ctgcatcgcg
     1441 caccgcatca agaactgccc tgatttcgtc ttcggtcagc cattcaccgg catacgggaa
     1501 agacacctgc gttccgtccg ccggaagtac cggcagcgac tgccactgac tgccggacag
     1561 aacaagttcc gctccggttc cggtcagttt ctgaatggcg gaggccaggt gcggcggcag
     1621 cacgccatcg tggcggttaa cgagtaacgt cgccagccgg ggcgcatcca gccgcacgta
     1681 aatgctgatc cagcccggat agttcgggtt acattggtca acatccgtct cttccggcca
     1741 gccctgcgcc agcagcaggt tacgggcgtg acgacaaatc agagcattca gcgtttcatt
     1801 cataattcat gaactccctg tatcgccgcc cacaggcggc gataacatca gtcgttttcg
     1861 tgtgtgttgt ttttcagcag gctgcgggcc tgatcaacca tcgcctgtcg gcgggtaacg
     1921 tattccggga gggggaaacc gtgatcccgg gcagcccatt cacgtttcca cagtgcctgt
     1981 tcagcgatct gagtcagggt ctccagcagt tcagcaggga cggtcacctg gcggttttcg
     2041 cgcacgttgc tgataatctc catcgccgcg gcggaagaaa taccggactc aatccgctag
     2101 tcttccggga tgtcctccag gtgcgccagg acggtcctga tttcatgatc ggacagggaa
     2161 atatccattc cgtcagcgag ggcctgaatc tcttccgggg accagataac cagcatcagc
     2221 ggcgtgtctc ccgaatactt tgctgccagc gcccggcata gcgtttcgca tgttccgtac
     2281 atactctctc ccctcaatct ttgaagcagg aatccagttc cagaatttcg gtatatgcct
     2341 ccatcaagag gcggtttgcg cgaaacaacg tctgacgcac cagacgttcc ggcagggtgt
     2401 cagcattaaa gctgtaccgc cgctccatct cattcatggt tgtacgcagg tcgcacatgg
     2461 cattggccac ctccgccact tcggcgcgaa gtttttcgtg tccggcatag ttcatttacg
     2521 cagcctccgg cataaaccag tcatcattcg cggccccctg ctgcatggcc cgctgtacgg
     2581 cagccagtcg ttgctgtccg gcacggtgat actgctcaag cagctcgata ccgatatacc
     2641 ggcgtccgga ctggagggcg gcgacgcagg ttgaaccgct gcctgcaaac gggtccagca
     2701 ctatcgcatt cgggtgtgtg aagctctcaa tcagcggttg caggctggta acgggctttt
     2761 ccgtcgggtg atggcggttg cccgaatatt tccagcccaa cacgtccggc agcgggtttt
     2821 gtggcagacg tggacggcct tttgccagga tgtaggcgca ttcgtggcga tagcccacat
     2881 atgcggcctt cgatgtgtag tttttggtga agaccaggtg accaacaacg ctgaatcccg
     2941 catttttcca ggcagacata aagcgatcga cgcggttcca gccatagaag ctcaccatca
     3001 gcgcgtcttt tttgagtacg cggtacattt cattacaggc cggttgcagc cattcatcag
     3061 ttttatcacc agcaatggta cgcccctgac ggtcacgaaa accgacgaga tatggcggat
     3121 cggtgaggat gaaatcaatg gcgttatcag gaatacgggc cataacatcg atgcagttac
     3181 cgaggacaaa acgggacata acagatcctt ctcttacaga gggcacccct gtggtgcccg
     3241 ttccccgctg cctgtgggct gttcatcgct tccggtaacg ggtcaggccg accgtggaat
     3301 accgcagccg tcgtcttttt gacagcaagg ttatgccgcg aaagcggctt gaagggcgtt
     3361 catcagaaaa gggcgacggg ctggcgtaca ctgtcaccga caccagccgg gagcggtaaa
     3421 agcgcccgtc ccgtgcccgc cggacgcggg acactgacca caggcagcgt ttatgccgcg
     3481 ccgcgtcagc cggcgcagta aaaagaggag cacggcgaca tcctttgtcg gaaaagaaac
     3541 gggcggttgc ccgcccgtct ccgtcatact tcatccgcca taatgcgcca cattgtcgac
     3601 tatccacgca ggtccggtga cttccggctg ctcatcgtca tctgtcgggg aagaccattc
     3661 aagttccccc cacagcacat tgacgagctc tccacgctca tacaactgcc agccacagaa
     3721 atcacagccc tgttcggcat accagtgctc cagcgtgcag ctgaaacggc ggcttaattc
     3781 tgcaataacg tcgctttccg gctgacacca cggagtgtcg aaatccacct gaatgaagtt
     3841 ttctccctga ctgctgatat tcaggtcata tccacaaggc cacttcacgc cataccgttc
     3901 cgtataccag tgatatgcag aaggaacacc gttcagaaca ccaccattaa agccgttcac
     3961 ttcaacatcc aggcgggtcg gcagaaccgt cagcaggtca aacggcatgg ttttttccgg
     4021 cactacgttg tcacacagac ggttccacca caccgataca tcctcgttac cccatatgtc
     4081 acaccagtca cctctttttg cggtgaaatg cacagtgatg gtttccctgg catcatccgg
     4141 taatccttcc caacgacgct ggccagtacc gctctgtaac cagagttcat gcagcatcag
     4201 gcagttttgt tcatccagca acacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc
     4261 gatattttcc gtcgatacag caccacgtcc ggcagccgtc actccggggc actgttcaga
     4321 gcggatattt tcagtgatct gcagaagtcc ggcacttccg gccagaaaca gctgaatacc
     4381 ttcatttgtg gcgcggcgat aaagcggtgt gactgcaccg ctggccagtc gtttaatctc
     4441 agcgatctgt gccggctcac taggaaaata catacgattc gagcaccagt ttggcatggg
     4501 aattctcctc tcacatgcgg cctccatcag gccgtgccgc acctgtaacc gggcgaccgc
     4561 atacgaatgc aggtgcggcc ccgaagccgc agcgcgtgga ggaggaatga cgaaacgtga
     4621 ccgggtgagg aggccggcga cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc
     4681 cggcacggcc tggttaaata gtatccgtct cagttcgtgg cgcaaaatga gaaaccaata
     4741 agaactcaaa atgaaccaca acaagaacat aaagagactc taaaagagca acaccgtttg
     4801 acatagactc aaaatgagtc acaataagaa acaataaaaa actcaaaaag atgcaccgac
     4861 ataaaggatg ctcaatttga gataaacaca aactcaaaac gaggcaaaaa gaatgctaaa
     4921 agtatcatgc gatgatggtt caactaacgt aaaactggcc tggcttgaag atggcgaggt
     4981 aagaaccagt ctgtcaggaa acagctttaa agaaggctgg aacccgggac tgtttaatgc
     5041 cggcaaagtt tacaattacg tcgtggatga aaaaaataca cctatgattt gggaagcact
     5101 gctgtcatag gtacaacaca tgtcagttat caatacagca ccaccaatct gcttgccata
     5161 catcatgcac tgctgacatc cggccttcag ccacaggatg tagaactgac agtaacatta
     5221 cctgtgaccg aattctttga taatgacaat caaccaaatg aagaaagaat agaaagaaaa
     5281 aaggcaaatg ttctccgtga gatatctctg aataaagggg aaacatttaa aattaaaaaa
     5341 gttaatgtaa tgcctgaatc tttgccagct gcatttgaat cactaaaaaa agataaagta
     5401 aacaaacttg aacgcagcct gattatcgat cttggaggaa ccactctgga ttgtggttta
     5461 attctcggtg catttgaagg catttctgaa atccgtggct attcagaaat cgggacatcc
     5521 cgcatcactc ataccgtcat gaatgcgctg acgaaagcct ccacaccatg taattatttt
     5581 attgctgatg ag
//
LOCUS       DAZSIO010000201         5590 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZSIO010000201 DAZSIO010000000
VERSION     DAZSIO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.204"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(1..279)
                     /locus_tag="ACHX5T_003668"
     CDS             complement(1..279)
                     /locus_tag="ACHX5T_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468680.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="ACHX5T_003669"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="ACHX5T_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR8468681.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="ACHX5T_003670"
     CDS             complement(1254..1670)
                     /locus_tag="ACHX5T_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468682.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="ACHX5T_003671"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="ACHX5T_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2887
                     /locus_tag="ACHX5T_003672"
                     /pseudo
     CDS             1837..2887
                     /locus_tag="ACHX5T_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2915..3004)
                     /locus_tag="ACHX5T_003673"
     tRNA            complement(2915..3004)
                     /locus_tag="ACHX5T_003673"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="ACHX5T_003674"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="ACHX5T_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR8468683.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="ACHX5T_003675"
     tRNA            3996..4071
                     /locus_tag="ACHX5T_003675"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..5495
                     /locus_tag="ACHX5T_003676"
                     /pseudo
     CDS             4233..5495
                     /locus_tag="ACHX5T_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1508 a         1326 c         1293 g         1463 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     4861 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     4921 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     4981 agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg
     5041 tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac
     5101 agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc
     5161 ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg
     5221 tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca
     5281 gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc
     5341 aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca
     5401 aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc
     5461 cttatacaat tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg
     5521 caaattacca ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg
     5581 aaaaacacct
//
LOCUS       DAZSIO010000202         5559 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZSIO010000202 DAZSIO010000000
VERSION     DAZSIO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.205"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            240..2672
                     /gene="hsdR"
                     /locus_tag="ACHX5T_003677"
     CDS             240..2672
                     /gene="hsdR"
                     /locus_tag="ACHX5T_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFR8468684.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="ACHX5T_003678"
     CDS             2739..4208
                     /locus_tag="ACHX5T_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR8468685.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..>5559
                     /locus_tag="ACHX5T_003679"
     CDS             4208..>5559
                     /locus_tag="ACHX5T_003679"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFR8468686.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     G"
BASE COUNT         1641 a         1312 c         1323 g         1283 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttgttgtc ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt
     4801 ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa
     4861 agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga
     4921 aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct
     4981 ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac
     5041 cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac
     5101 cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact
     5161 tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga
     5221 aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag
     5281 cgatgaggaa aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat
     5341 agttaatatt aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc
     5401 acctgatatt attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc
     5461 aggagtcaaa gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg
     5521 cccgtcatta tccaaagttt ttttagcaga gtataatgg
//
LOCUS       DAZSIO010000203         5558 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZSIO010000203 DAZSIO010000000
VERSION     DAZSIO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.206"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..642
                     /locus_tag="ACHX5T_003680"
     CDS             <1..642
                     /locus_tag="ACHX5T_003680"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HFR8468687.1"
                     /translation="VVGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAIT
                     TGKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQN
                     AVLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCA
                     LGLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(696..1301)
                     /gene="azoR"
                     /locus_tag="ACHX5T_003681"
     CDS             complement(696..1301)
                     /gene="azoR"
                     /locus_tag="ACHX5T_003681"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR8468688.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1502..5404
                     /gene="hrpA"
                     /locus_tag="ACHX5T_003682"
     CDS             1502..5404
                     /gene="hrpA"
                     /locus_tag="ACHX5T_003682"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR8468689.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLRYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1377 a         1359 c         1540 g         1282 t
ORIGIN      
        1 gtcgtaggcg cgtgcattgt gttgatggag ctaatgatga tggttcagtt atggtggtca
       61 gtctggttat gttggccagt attatcgcta ctcattattg gccgtgggtg cggtgggctt
      121 ggcgcgataa caacagggaa agatagtcag gggaaactcc cgcccgccgt ttactggctg
      181 acattgccct ggcgcatcgg gatgtggcta tctatgcgtt ggttttgtcg tcgcctggag
      241 ccggtgagca aaatgactgc tggtgtttat ttaagggcgt ttccacgaca tattccggca
      301 cagaatgcgg ttctggacgt cacctttgaa ttccctcgtg gacgagcgac aaaagatcga
      361 ctctatttct gtgtaccgat gttggatctg gtggttccgg aagaggggga gctccgacag
      421 gccgtggcga tgctggaaac attacgcgaa gagcaaggca gcgttctggt ccattgcgca
      481 ttgggattat cgcgcagtgc gctggtagtg gcggcatggc tgttatgtta cggacactgt
      541 aaaacagttg atgaagcgat tatctttatt cgagccagac gctcgcatat tgtgcttaag
      601 gaagagcaca aagcgatgtt gaaattatgg gaaaacaggt aagtggattg agatgtggac
      661 tgaatatcta cagtccacat caagaccgtg tccggttatg cagcaacaat gctgtcgatg
      721 gctgcttttg cgtcagactg tgctttcgct gccatttccg gaccgtatgc gatcccttcg
      781 gcgaagacaa atttcacatc ggtaatgccg ataaagccga ggaacgtgga cagatacggc
      841 gtcaccaggt ccgttggtcc atctttgtgg atcccaccgc ggctggtaat aacgatggct
      901 tttttacccg ttaccagacc ttccggaccg ttctcggtat agcggaaagt aacgcctgcg
      961 cgtgcaacca ggtcaaaata gtttttcaac tgagttgaga tgttgaagtt atacatcggt
     1021 gccgcaataa cgataacgtc gtgggctttc agctcggcaa tcagctcatc ggaaagcgcc
     1081 agagcttcct gctgacgcgg agtcagcggc gcatcgctcg gacgcagagc gccaaccagt
     1141 tcgccatcca gtaccggaat cggatttgca gctaggtcgc gaacggtgat ttcatcagcg
     1201 gagtgctttt cgctccattg ttcaacaaaa taatcggaca actgattaga ctgagagtac
     1261 cctgccagga tgctggattt aagaactaat accttgctca tggtgtttcc ttatagatgt
     1321 ttgaatggac gatgccccgt tgcttgctga cactttattc acaatcctgc cacagagatc
     1381 gcgcaataaa tcgaagtcta tgttcgaatt tattgaacaa cgcatagaaa gccgcgatgt
     1441 ggtactctat atctatcatt taaaagaaaa ttaatcaggc agactactgc ccactaacgt
     1501 tatgacagaa caacaaaaat tgacctttac tgccttgcaa cagcggctgg attcgctgat
     1561 gctgcgcgac agactgcgtt tttctcgccg tctgcattgt gttaagaagg ttaaaaatcc
     1621 tgatgcacaa caggccattt tccaggagat ggcgaaagag attgaccagg cggcagggaa
     1681 agtcctgctg cgtgaagcgg cacgaccgga aatcacttat cctgacaatt taccggttag
     1741 tcagaaaaaa caggacatac tcgaagcgat tcgtgatcac caggtggtga tcgtcgccgg
     1801 ggaaaccggc tccggtaaaa cgactcagct accgaaaatc tgtatggagc tggggcgcgg
     1861 tattaaaggg ctgatcggtc atacccagcc gcgtcgcctg gcggcacgaa cggtggccaa
     1921 ccgtattgcg gaagagctga aaacggagcc gggcggttgc atcggttaca aagtgcgttt
     1981 cagcgatcac gtaagtgata acacgatggt caagctgatg accgacggga tcctgctggc
     2041 ggagatccag caagaccgcc tgctgatgca gtacgacacc atcattattg acgaagcgca
     2101 cgaacgcagc ctgaatatcg attttctgct cggctatttg aaagagttgc tgccgcggcg
     2161 tcctgaccta aaaatcatta tcacttccgc gactatcgac ccggaacgct tttcgcgcca
     2221 ctttaataat gcgccgatta ttgaagtctc cggtcggacc tatccggtgg aagtgcgcta
     2281 tcgcccgatt gttgaagaag ccgatgacac cgagcgcgat cagttgcagg cgatttttga
     2341 cgccgtagac gaactaagcc aggaaagccc cggcgacatt ctgatcttta tgagcggcga
     2401 gcgggaaatt cgcgataccg ccgatgcgct gaacaagctg aacttacgcc ataccgaaat
     2461 cttgccgctt tatgcgcggc tttcgaacag cgaacagaac cgcgtgttcc agtcgcacag
     2521 cggacggcgc attgtgctgg cgaccaacgt cgcggaaacc tcgctgaccg taccggggat
     2581 taaatacgtc atcgaccccg gtacagcgcg tatcagccgc tacagctatc gcaccaaagt
     2641 gcagcgtttg ccgattgaac ccatttcgca ggcgtctgcc aatcagcgta aaggccgctg
     2701 tggtcgtgtg tccgaaggga tctgtattcg tctctattcc gaagacgatt tcctctcgcg
     2761 cccggagttt accgatccgg agattctgcg taccaacctg gcctcggtta ttttgcagat
     2821 gaccgcgctg gggctgggcg atatcgctgc gttcccgttt gtcgaagcac cggataaacg
     2881 caatatccag gatggcgtgc gtctgctcga agaactgggc gcgatcacca cggatgaaca
     2941 ggccagcgcc tataaactga cgccgctcgg tcgccagctc tcgcagttgc ctgtcgaccc
     3001 acgtctggcg cgtatggtgc tggaagcgca aaaacatggc tgcgtgcgtg aggcgatgat
     3061 tatcacgtcc gcgctctcca tccaggatcc gcgcgaacgt ccgatggaca aacagcaggc
     3121 atcggacgaa aaacatcgtc gcttccacga caaagaatcc gacttcctcg cgtttgtaaa
     3181 tctgtggaat tatcttggcg agcagcaaaa ggcgctttct tccaacgcct tccgtcgcct
     3241 gtgtcgtacc gattacctca actatctgcg cgtgcgcgaa tggcaggata tctacaccca
     3301 gttgcgccag gtggtgaaag aacttggcat tccggtcaac agcgaaccgg cggagtatcg
     3361 tgaaattcac atcgccttac tgaccggttt gctttcccat atcggcatga aagatgccga
     3421 taaacaggaa tataccggtg cacgtaacgc gcgtttctcc atcttccccg gttctggttt
     3481 attcaaaaag ccgccgaaat gggtaatggt ggcggaactg gtagaaacca gccgcctgtg
     3541 ggggcgcatt gctgcgcgta tcgacccgga atgggtagaa cccgttgctc agcatttgat
     3601 taaacgcacc tacagcgaac cgcactggga acgggcgcag ggcgcggtga tggcaacgga
     3661 aaaagtcact gtttatggtt tgccgattgt tgctgcgcgc aaggtcaact acagccagat
     3721 cgattcggcg ttatgtcgtg aactctttat tcgccacgct ctggtggaag gtgactggca
     3781 gacgcgtcac gcattcttcc gtgaaaacct gaaactgcgg gccgaagtgg aagagctgga
     3841 acacaaatca cgtcgccgcg atattctggt tgatgacgaa acgctgtttg agttctacga
     3901 ccagcgcatc agccacgatg taatctccgc tcgtcacttc gatagctggt ggaaaaaagt
     3961 cagccgcgaa acgcctgatt tgctcaactt tgaaaagagc atgttgatca aagaaggggc
     4021 ggaaaaaatc agcaagctgg attacccgaa cttctggcat cagggcaatc tcaagctgcg
     4081 tttgagatat cagtttgagc caggcgcgga cgcggacggt gtgaccgtac atattccgct
     4141 gccgttactt aaccaggttg aggaaagcgg gtttgaatgg cagatccccg gtctgcgccg
     4201 cgaattggtg attgctctga ttaaatcgtt gccgaaaccg gtacgccgta attttgtgcc
     4261 tgcaccaaac tacgccgaag cgtttttagg ccgcgtcaca ccgctggagt taccgttgct
     4321 cgacagcctt gagcgcgagt tacggcggat gaccggcgtt accgttgacc gcgaagactg
     4381 gcactgggat caggtgcccg atcacctgaa aatcaccttc cgcgtggtgg atgacaaaaa
     4441 caagaagcta aaagaagggc gctcgctaca agatctgaaa gatgcgctga aaggcaaagt
     4501 gcaggaaacg ctgtccgcgg tggcggatga cggtatcgag cagagcggct tacatatctg
     4561 gagttttggt cagctgccgg aaagttacga acagaagcgt ggcaactata aagtgaaggc
     4621 gtggccggcg ctggtggatg aacgcgacag cgtgacgatc aaactgtttg ataacccgct
     4681 ggagcaaaaa caggcaatgt ggaatggtct tcgccgtcta ctgctgctaa atatttcctc
     4741 gccgatcaag tatttacatg agaagttacc aaacaaagcc aagctggggc tgtactttaa
     4801 cccgtatggc aaagtgctgg agctgatcga cgactgtatc tcctgcggtg tggatcaact
     4861 gatcgacgcc aatggtggcc cggtctggac ggaagaaggc tttgctgcgc tgcatgaaaa
     4921 agtgcgcgcc gaactgaatg acacggtggt ggatattgcg aagcaggtcg agcaaatcct
     4981 cactgcggtg ttcaatatca acaaacgtct gaaagggcgg gtggatatga ccatggcact
     5041 ggggctttct gatattaaag cgcagatggg cgggttggta tatcgcggtt ttgtcactgg
     5101 taacggcttc aaacggctgg gcgacacgct gcgatatttg caggcgattg aaaaacgact
     5161 ggaaaaactg gcggttgatc cacatcgcga tcgtgcacag atgctgaaag tcgaaaacgt
     5221 ccagcaggcg tggcagcaat ggatcaacaa actgccgcct gcacgtcgtg aggatgaaga
     5281 cgtgaaagag atccgttgga tgatagaaga gctgcgcgtt agttacttcg ctcaacaact
     5341 tggtacgcct tatccgattt cagataagcg tattttgcag gcgatggagc agattagcgg
     5401 ttaaccctgc tatttgccta ataaatctaa aacccggtaa gcatttagcg ccgggttttt
     5461 tattaattct aaaacggtaa ggacaaaaat tcaggaattc agaaaaatac aattctcagc
     5521 tgcaagatga ataatgttta tctacagcat ttccttaa
//
LOCUS       DAZSIO010000204         5520 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZSIO010000204 DAZSIO010000000
VERSION     DAZSIO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.207"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..1479
                     /gene="ipaB"
                     /locus_tag="ACHX5T_003683"
     CDS             <1..1479
                     /gene="ipaB"
                     /locus_tag="ACHX5T_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HFR8468690.1"
                     /translation="ILGEKSLTALTNKITAWKSQQQARQQKNLEFSDKINTLLSETEG
                     LTRDYEKQINKLKNADSKIKDLENKINQIQTRLSELDPESPEKKKLSREEIQLTIKKD
                     AAVKDRTLIEQKTLSIHSKLTDKSMQLEKEIDSFSAFSNTASAEQLSTQQKSLTGLAS
                     VTQLMATFIQLVGKNNEESLKNDLALFQSLQESRKTEMERKSDEYAAEVRKAEELNRV
                     MGCVGKILGALLTIVSVVAAAFSGGASLALAAVGLALMVTDAIVQAATGNSFMEQALN
                     PIMKAVIEPLIKLLSDAFTKMLEGLGVDSKKAKMIGSILGAIAGALVLVAAVVLVATV
                     GKQAAAKLAENIGKIIGKTLTDLIPKFLKNFSSQLDDLITNAVARLNKFLGAAGDEVI
                     SKQIISTHLNQAVLLGESVNSATQAGGSVASAVFQNSASTNLADLTLSKYQVEQLSKY
                     ISEAIEKFGQLQEVIADLLASMSNSQANRTDVAKAILQQTTA"
     gene            1442..2590
                     /gene="ipaC"
                     /locus_tag="ACHX5T_003684"
     CDS             1442..2590
                     /gene="ipaC"
                     /locus_tag="ACHX5T_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HFR8468691.1"
                     /translation="MLQKQFCNKLLLDTNKENVMEIQNTKPTQILYTDISTKQTQSSS
                     ETQKSQNYQQIAAHIPLNVGKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKD
                     LSEMSHTLTPENTLDISSLSSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKS
                     AAENIVRQGLAALSSSITGAVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKE
                     LAGSKLGLNKQIDTNITSPQTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDK
                     IDTQRRTYELNTLSAQQKQNIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASS
                     KQAEEASQVSKEASQATNQLIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            2641..3639
                     /gene="ipaD"
                     /locus_tag="ACHX5T_003685"
     CDS             2641..3639
                     /gene="ipaD"
                     /locus_tag="ACHX5T_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HFR8468692.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            3648..>5520
                     /gene="ipaA"
                     /locus_tag="ACHX5T_003686"
     CDS             3648..>5520
                     /gene="ipaA"
                     /locus_tag="ACHX5T_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HFR8468693.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     "
BASE COUNT         2065 a         1120 c          925 g         1410 t
ORIGIN      
        1 atactcggtg aaaaatcttt aactgcatta acaaataaaa ttactgcttg gaagtcccag
       61 caacaggcaa gacagcaaaa aaacctagaa ttctccgata aaattaacac tcttctatct
      121 gaaactgaag gactaaccag agactatgaa aaacaaatta ataaactaaa aaacgcagat
      181 tctaaaataa aagacctaga aaataaaatt aaccaaattc aaacaagatt atccgaactc
      241 gacccagagt caccagaaaa gaaaaaatta agccgggaag aaatacaact cactatcaaa
      301 aaagacgcag cagttaaaga caggacattg attgagcaga aaaccctgtc aattcatagc
      361 aaacttacag ataaatcaat gcaactcgaa aaagaaatag actctttttc tgcattttca
      421 aacacagcat ctgctgaaca gctatcaacc cagcagaaat cattaaccgg acttgccagt
      481 gttactcaat tgatggcaac ctttattcaa ctagttggaa aaaataatga agaatcttta
      541 aaaaatgatc tggctctatt ccagtctctc caagaatcaa gaaaaactga aatggagaga
      601 aaatctgatg agtatgctgc tgaagtacgt aaagcagaag aactcaacag agtaatgggt
      661 tgtgttggga aaatacttgg ggcactttta actatcgtta gtgttgttgc agcagctttt
      721 tctggaggag cctctctagc actggcagct gttggtttag ctcttatggt tacggatgct
      781 atagtacaag cagcgaccgg caattccttc atggaacaag ccctgaatcc gatcatgaaa
      841 gcagtcattg aacccttaat caaactcctt tcagatgcat ttacaaaaat gctcgaaggc
      901 ttgggcgtcg actcgaaaaa agccaaaatg attggctcta ttctgggggc aatcgcaggc
      961 gctcttgtcc tagttgcagc agtcgttctc gtagccactg ttggtaaaca ggcagcagca
     1021 aaacttgcag aaaatattgg caaaataata ggtaaaaccc tcacagacct tataccaaag
     1081 tttctcaaga atttttcttc tcaactggac gatttaatca ctaatgctgt tgccagatta
     1141 aataaatttc ttggtgcagc gggtgatgaa gtaatatcca aacaaattat ttccacccat
     1201 ttaaaccaag cagttttatt aggagaaagt gttaactctg ccacacaagc gggaggaagt
     1261 gtcgcttctg ctgttttcca gaacagcgcg tcgacaaatc tagcagacct gacattatcg
     1321 aaatatcaag ttgaacaact gtcaaaatat atcagtgaag caatagaaaa attcggccaa
     1381 ttgcaggaag taattgcaga tctattagcc tcaatgtcca actctcaggc taatagaact
     1441 gatgttgcaa aagcaatttt gcaacaaact actgcttgat acaaataagg agaatgttat
     1501 ggaaattcaa aacacaaaac caacccagat tttatataca gatatatcca caaaacaaac
     1561 tcaaagttct tccgaaacac aaaaatcaca aaattatcag cagattgcag cgcatattcc
     1621 acttaatgtc ggtaaaaatc ccgtattaac aaccacatta aatgatgatc aacttttaaa
     1681 gttatcagag caggttcagc atgattcaga aatcattgct cgccttactg acaaaaagat
     1741 gaaagatctt tcagagatga gtcacaccct tactccagag aacactctgg atatttccag
     1801 tctttcttct aatgctgttt ctttaattat tagtgtagcc gttctacttt ctgctctccg
     1861 cactgcagaa actaaattgg gctctcaatt gtcattgatt gcgttcgatg ctacaaaatc
     1921 agctgcagag aacattgttc ggcaaggcct ggcagcccta tcatcaagca ttactggagc
     1981 agtcacacaa gtaggtataa cgggtatcgg tgccaaaaaa acgcattcag ggattagcga
     2041 ccaaaaagga gccttaagaa agaaccttgc cactgctcaa tctcttgaaa aagagcttgc
     2101 aggttctaaa ttagggttaa ataaacaaat agatacaaat atcacctcac cacaaactaa
     2161 ctctagcaca aaatttttag gtaaaaataa actggcgcca gataatatat ccctgtcaac
     2221 tgaacataaa acttctctta gttctcccga tatttctttg caggataaaa ttgacaccca
     2281 gagaagaact tacgagctca ataccctttc tgcgcagcaa aaacaaaaca ttggccgtgc
     2341 aacaatggaa acatcagccg ttgctggtaa tatatccaca tcaggagggc gttatgcatc
     2401 tgctcttgaa gaagaagaac aactaatcag tcaggccagc agtaaacaag cagaggaagc
     2461 atcccaagta tctaaagaag catcccaagc gacaaatcaa ttaatacaaa aattattgaa
     2521 tataattgac agcatcaacc aatcaaagaa ttcgacagcc agtcagattg ctggtaacat
     2581 tcgagcttaa tatatccaag agccataata atatatggct cttcctgtaa ggaaataacc
     2641 atgaatataa caactctgac taatagtatt tccacctcat cattcagtcc aaacaatacc
     2701 aacggttcat caaccgaaac agttaattct gatataaaaa caacgaccag ttctcatcct
     2761 gtaagttccc ttactatgct caacgacacc cttcataata tcagaacaac aaatcaggca
     2821 ttaaagaaag agctttcaca aaaaacgttg actaaaacat cgctagaaga aatagcatta
     2881 cattcatctc agattagcat ggatgtaaat aaatccgctc aactattaga tattctttcc
     2941 aggcacgaat atccaattaa taaagacgca agagaattat tacattcagc tccgaaagaa
     3001 gccgagcttg atggagatca aatgatatct catagagaac tgtgggctaa aattgcaaac
     3061 tccatcaatg atattaatga acagtatctg aaagtatatg aacatgccgt tagttcatat
     3121 actcaaatgt atcaagattt tagcgctgtt ctttccagtc ttgccggctg gatctctccc
     3181 ggaggtaacg acggaaactc cgtgaaatta caagtcaact cgcttaaaaa ggcattggaa
     3241 gaactcaagg aaaaatataa agataaaccg ctatatccag caaataatac tgttagtcag
     3301 gaacaagcaa ataaatggct tacagaatta ggtggaacaa tcggcaaggt atctcaaaaa
     3361 aacgggggat atgttgtcag tataaacatg accccaatag acaatatgtt aaaaagctta
     3421 gataatctag gtggaaatgg cgaggttgtg ctagataatg caaaatatca ggcatggaat
     3481 gccggattct ctgccgaaga tgaaacaatg aaaaataatc ttcaaacttt agttcaaaaa
     3541 tacagtaatg ccaatagtat ttttgataat ttagtaaagg ttttgagtag tacaataagc
     3601 tcatgtacag atacagataa actttttctc catttctgag gtgcgccatg cataatgtaa
     3661 ataatactca agcgccaaca ttcttatata aggcaacttc accatcatca acagaataca
     3721 gcgagttaaa aagcaaaata tccgatatcc atagttcgca aacttctcta aaaacaccag
     3781 catcagtgtc tgaaaaagaa aactttgcaa cgtcttttaa tcagaaatgt cttgattttt
     3841 tattttcttc ctcagggaaa gaagatgtgt taagaagcat ttattccaac tcaatgaatg
     3901 cgtatgccaa aagcgagatt ctcgaatttt caaatgtttt gtactcctta gtacatcaaa
     3961 atggtcttaa ttttgaaaac gaaaagggac ttcaaaaaat tgtcgcacag tattcggaac
     4021 taattataaa agataaatta tcccaagatt ctgcctttgg accatggtcg gcaaagaata
     4081 agaaactcca tcaattacga caaaacattg agcacagact tgcactatta gcacaacaac
     4141 acacatctgg tgaagcttta tcattgggac aaaaactcct caatactgaa gtatcatcat
     4201 ttatcaagaa taatattctt gctgaattaa agttaagtaa tgaaactgtt tcatctctca
     4261 aactagatga tttagttgac gcacaggcaa aacttgcctt tgatagtttg cgcaatcaac
     4321 gtaaaaatac tattgatagt aaaggatttg gtataggtaa actgtcaaga gacttaaata
     4381 cagtagccgt gtttcctgag ctgttgagaa aagtccttaa tgatatttta gaagatataa
     4441 aagattcgca tcctatccaa gatggcctcc ctacacctcc cgaagatatg ccagatggcg
     4501 gaccaacccc cggagccaat gagaaaacat cccaacctgt aattcactat catataaata
     4561 atgataatag aacttacgat aatagagttt ttgacaacag agtatatgac aatagctatc
     4621 acgagaaccc agaaaatgat gcacagtctc ctacttctca gacaaacgat ctattatccc
     4681 gtaacggaaa ctcattacta aatccacaaa gagcactagt tcaaaaagta acttccgttc
     4741 taccacactc tatatcagat actgtccaga catttgcaaa taattcagct ttagaaaagg
     4801 ctttcaacca tactccagat aattcggatg gaataggttc agacctgtta actacgagta
     4861 gtcaagaaag atctgcaaat aactctcttt ctcggggaca caggcctctg aacatacaga
     4921 actcttcaac caccccccct ctccacccgg aaggagtgac aagcagtaat gataactcat
     4981 cagatacaac taaaagtagc gcttctcttt ctcatagagt agcttcgcaa atcaataaat
     5041 tcaactcaaa cactgattca aaagtacttc agactgattt tttatcaaga aatggagaca
     5101 catatttaac acgggaaacg atatttgaag cttcaaaaaa agtaacaaac tccctaagta
     5161 atcttatatc tctcattgga actaaatcag gaacacaaga acgagagtta caggaaaaat
     5221 caaaggacat tacaaaatcc acaacagaac atagaataaa caacaaatta aaagttacag
     5281 atgcaaatat aagaaactac gtaacagaaa ccaacgcaga tacaattgat aaaaatcatg
     5341 cgatctatga aaaggcaaaa gaagtatcta gcgccctcag caaggtattg tcaaaaattg
     5401 acgatacctc tgcagaatta cttacagatg atatatctga tttaaaaaat aacaatgata
     5461 ttacagctga aaacaataat atatataaag cagcaaaaga tgtaaccact tccctatcaa
//
LOCUS       DAZSIO010000205         5506 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZSIO010000205 DAZSIO010000000
VERSION     DAZSIO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.208"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACHX5T_003687"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACHX5T_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR8468694.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="ACHX5T_003688"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="ACHX5T_003688"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR8468695.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="ACHX5T_003689"
     CDS             complement(3736..4092)
                     /locus_tag="ACHX5T_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR8468696.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="ACHX5T_003690"
     CDS             complement(4096..4512)
                     /locus_tag="ACHX5T_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR8468697.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACHX5T_003691"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACHX5T_003691"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR8468698.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1290 a         1456 c         1444 g         1316 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttg
//
LOCUS       DAZSIO010000206         5485 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZSIO010000206 DAZSIO010000000
VERSION     DAZSIO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.209"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..1630)
                     /locus_tag="ACHX5T_003692"
     CDS             complement(<1..1630)
                     /locus_tag="ACHX5T_003692"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR8468699.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPW"
     gene            complement(1646..2541)
                     /locus_tag="ACHX5T_003693"
                     /pseudo
     CDS             complement(1646..2541)
                     /locus_tag="ACHX5T_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
     gene            complement(2541..3146)
                     /locus_tag="ACHX5T_003694"
     CDS             complement(2541..3146)
                     /locus_tag="ACHX5T_003694"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR8468700.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3317..>5485)
                     /locus_tag="ACHX5T_003695"
     CDS             complement(3317..>5485)
                     /locus_tag="ACHX5T_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFR8468701.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1581 a         1364 c         1141 g         1399 t
ORIGIN      
        1 cccacggttt accatcttta tgactggtta acaccccggc aatcagctcc agttgtgggt
       61 gccacggctg cccggtgtag atgagaatgc cacccggttc gatggcattg gctagacctg
      121 cgagtgagtt ttttacctgc tcattttcgg gaaaaagctc atataggcca gagacaatcg
      181 ccagcgtagg ccgcggagtt aacgcgctga gttcctccgg gttaaacgca tcgccctgtt
      241 caaaacgcac ccgcccagac attccccgtt gggcaatcat ctcttgcccc tgtgcaacat
      301 ttaactcact gtaatcacgt aacaaaatat cgcttacggc aggctcgttt gccagcgcat
      361 ccagtacata gcgtccatgc cctgcggcaa tgtcaaccac gcggacggct aaacctttgg
      421 cgtggagatc ggcaacggct tgtttaatca atatttgcaa atgggttttg cgctggcgaa
      481 taccgcgcca tccaacactg ttcaggtaga ttttgtcgat taaacgcccg aatgcgttac
      541 tgccttgcgg ttgattttga tagacataat ccagggaact gcctgaatca aagccggtac
      601 tcattccgag atgcaggccc gcagagtgcg caccgaacaa tttcatcgcc tttcgcataa
      661 agcgatacgc taaatcaacc ggcgataatg gcacgggtcc accagaaagg agccgccatc
      721 gatccgctga tggtcctgtg cggtcttcat gctgataatc aaatttttgc gatttgttag
      781 catataagcg gcagataaag ctttgcattt tttcaaatgc ctgcgccctg ttctcttcac
      841 ccaatgtgtc gtgataaaag cctggcagca gatgcagctc tttcagaggg ctacgtaatc
      901 tctgataaaa atcaatctgc ggttggcgat gcaccacata gtcatcacct gatatcagaa
      961 gttgcgtggg gagcgtaatc gccgccgcat cgctaacaat acgttcagac gttttataga
     1021 gatcgagcaa gatattgacg gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg
     1081 ccacccgttg ccgatcgtgg gtcaaatagc gccctttcac ataggaatta ataaaaaata
     1141 gaccacgcag acgatgccat aacgccagcg caggacgtgc cagcggcaca tacaatttaa
     1201 ccttaaaggc cggagaagcc agcaccagcc cacgaattgc aggtgcataa tcatgaaccc
     1261 atgtggcaac aagcactgcg ccgacgcttt gcgcgatcac taccacctct tccagtccga
     1321 cctggctgtc gctggcagca aaacggacaa attcatcgac atcctgcact gagcgcataa
     1381 gagacgggct ataaccacgc ggccccgaat tttttccatg cccccgggca tcccaggcat
     1441 aaaaagcagt atcaggcatc gccagttcat caacgagatg ttgtagacga ccagaatgtt
     1501 catgcccgcg atgaaataag acgatgacct ttttcgcccc tggctgtaaa gtgggccagt
     1561 gccgataata caatgctgta ttatcactgg tggtaaaaaa atgttcccca gggatgcgtg
     1621 aattttccat tttctctatt ttccttcagt aacagcagta gcgaataaaa taaaaaaata
     1681 ccggagcggt gaaaattaat gagtcaatcc tgtcaaggag tccgccatgt cctagtaata
     1741 gttttccact atctttaaca ccaatatctc gtttgatggc tgacatcact acatcgccgc
     1801 aaaaaccact aataccaatt aacaaacccg ctaataatgc ctgtaatgta tttagcggtg
     1861 tcagcaacgg tccaataatc agtgacgcaa tcatggtggt gattacgccc cccagcagac
     1921 cttccagcgt ttttcccggg ctgactttgg ggaccacttt tcttctgccg caggatttcc
     1981 cccataaata ctgtgcaata tcgtttgact cggttaacgc caaaagaaat aacaccagta
     2041 acgccccgcc ctggatattt atggttggca acattaatag ccaggcggca tgactcaagg
     2101 caaaaacggt cattatccat ccccaaaaaa tggcgctcac ggtatgctga aaaccggagg
     2161 ggtcacccac aaaaactcgc caggtggcta atatcagaaa cccagtcaaa ggtataaata
     2221 acagaaacag ctcaaagcaa ttaaaaccga taagcaaata gttaagagga ataacccaat
     2281 ataaccaacg cgaaaaatgt acggatataa gcgtacaata ttctttcaat gccagaaaac
     2341 ttatcagcgt aaaaaacgtc aacgtcatcc agcgtgggcc tgaaatcacc agtgaaaaac
     2401 aaatgacgat gccccaccag gtacgaatac gtaatgtaac ttcatcccct tttctctcag
     2461 gtaaccgcca tagcagaaaa cggtttatca gcttgctaaa attaacaacg cgaaaagtgt
     2521 tgccagagat ttttccagca ttatctttca gcgctcataa gaagcacact ccgacaacga
     2581 ttaatcgcag tacagataag cagaattgaa gcaatgctcc ataaaagatt attccattgc
     2641 atccactgtg gataaatggc aacggccaga ccccacagac caaatattaa cgcgcgatca
     2701 cttttgccaa atggaccggc ataactgcga acaccgttaa tcgtctgcgc gagtaaaccg
     2761 caaaactcgg tcaatatggt gcaaaataac atgagtatca cgagtgatgc gttactttcc
     2821 ggtaaaaata aaaacggtaa atagagcgca atatcggaaa taacatcgcc agtctcattc
     2881 aaaatcgccc ccagacgtgt ttgctgattg cactcacgcg ccaacatgcc atccagcgca
     2941 ttgagcgcca tacggataaa aagcacgatg ggcaatagca aaaagaggat gggttgtgcc
     3001 accaacacca acagcaatcc ggtaaaaaga gaaagcgcca gtgccgtaag agtgatgtga
     3061 ttcgctgtaa cgtggtgctt ataaagccaa aacatcgtcg gccttaacag cgactgaaag
     3121 agcggtttta tctggtagag cgtcattatc aatccttgat aattcggttt gcgctatggt
     3181 gcatcaattc ccctgacgga gatatcgcaa cacgccgctt cattaaaaat tcagaatata
     3241 tcaaggtatt gggggctcac tgtcaggaat gattatcccc aataaatcaa gtagttaaaa
     3301 attattacgc tgttattcaa tacaactcat caagtttcct catcgcttgt tcccgtattg
     3361 cgcgcgtctt accgttaagc gccagctggc ggactaaatc cagttcaggg ttaacaagct
     3421 ctaacgccag caaaggatca cgtttcagcc tcgccttcaa tacatcaact ggaaagcggg
     3481 gattattcat cgccacaatc caccactcgg gatcaatttc tgccgccagt gccgcagagg
     3541 gggtgtggcg gttgctaagt gcgccgaact tgaaaaatgt gagattgctt ttcgccagtg
     3601 tactccacca tatacgatcc ggttgttttg ccagtacatc cagttgtgct aacgcatcgg
     3661 ggtcgagtgc ataataaaca cgtaatgctt catcaacctg cgcacgggag ctattttcct
     3721 gcctcagaaa aaacttcgcc caaatatcat tgcgtggcgt gttttgctgt aattccttga
     3781 gaacaacagc gggtaaatta tcccaactaa ctaatgcact ctctaaaaag tcccagtctt
     3841 ccaaacgtat cagggcggcg gcatttaata gcatctggtc atcaacttcg ccgtggctaa
     3901 tcagctcaga caaaatcata tcagcgctgg tttcatatgc atctgaatct gctttcggat
     3961 tatttttccg atgcgataaa gcacgttgcc aaattttttg tttcatcgac gtgctcagat
     4021 actgattttt cgccagatta ctccacccct caggatcgtt gaaacgtgtc aataaccgtt
     4081 gtaaagcagc gtcagagtga attacctgca tatcccgaca gcaaaagtag gcaggggatt
     4141 ttttctccat ccttttaaca aaattaaact gtaagttatc tggcaatgct aaaaataaca
     4201 cacgtattag atcatcgtca ttctcgaatt tttggtataa ataggctgca actttgtcac
     4261 tctcttgtgg cgtcatccgc aataattgac catggtgttt catctctgcc agcttgaacg
     4321 ctaagcttgt gacaacattg tttgatgaat cgttcgccaa ctgcattgcc tgattgtaag
     4381 tgagttgtcc tccagcaccg acctgacgaa tagaggaatc atcactgcgg atatagccat
     4441 caataatgga ttcgggcaat aaccacgcat ctttataaag ctcctgaatg atcatatccc
     4501 aatctctttt cgccagagtc atgacgatat ccagagggag attttcacgt agcgcaagat
     4561 tcaacggacc gacaccatca ggtagacgac tcattcgcgt cattatttcg acagagagcg
     4621 gttcacggtc gagcaacccc agaacaacag gctggtaacc caattgctga tcggtcaact
     4681 ccagaatctc attgtcaggc atatatctcg ccatactttc aatcgtttgt tcatcattgc
     4741 tattaataag ttgtttgcgt aggttagctg aaattccttt aagccttggg agagttcgca
     4801 ttatggcaag atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg ttgccttcat
     4861 ccagcatttg caattcctgc actaactcag gagaaggctt agggcgaaac agcgcttctt
     4921 ttttcatctg attacagaca ttccctgcca tgcgaatatt gcttcgctta aaaatttctt
     4981 tcagagcatc ttcatcattt attagacccc aataatatgc atcaggattt attatcatca
     5041 gcgctttttt gacattttca gtgtcatttt tgtcactcgc acggaccatg aattcactat
     5101 ttgtcgaaga cgcaacaaca ggtgcaatta tgtcattgat aaatacccca cgcgcattaa
     5161 tactcccctt aatcgtagac ggattatcgt aatatgagtc gataaatacc agtccacttg
     5221 catttatcga accggtaatt aacagtggtg aatccgtaac tatattcgtt gccgtcacgt
     5281 taccgagaat aacccatgga ttagaaagag agaaagtgga aatattacct ttgactgtca
     5341 aattgccaag aacaatcaat ttttgattac tctctatatt accttctacc actaaatcgc
     5401 cacgatgcac tttccagtcc gatgcagata aaccgaaggg gacaagattc acgctactgt
     5461 tgatagcttg ttcaacttgt tgttt
//
LOCUS       DAZSIO010000207         5466 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZSIO010000207 DAZSIO010000000
VERSION     DAZSIO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.210"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..312)
                     /locus_tag="ACHX5T_003696"
     CDS             complement(<1..312)
                     /locus_tag="ACHX5T_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468702.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LV"
     gene            413..700
                     /locus_tag="ACHX5T_003697"
     CDS             413..700
                     /locus_tag="ACHX5T_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8468703.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            672..2201
                     /locus_tag="ACHX5T_003698"
     CDS             672..2201
                     /locus_tag="ACHX5T_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFR8468704.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(2371..3588)
                     /locus_tag="ACHX5T_003699"
     CDS             complement(2371..3588)
                     /locus_tag="ACHX5T_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8468705.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(3955..4941)
                     /gene="ycdU"
                     /locus_tag="ACHX5T_003700"
     CDS             complement(3955..4941)
                     /gene="ycdU"
                     /locus_tag="ACHX5T_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFR8468706.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(4938..5466)
                     /locus_tag="ACHX5T_003701"
                     /pseudo
     CDS             complement(4938..5466)
                     /locus_tag="ACHX5T_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         1676 a         1301 c         1099 g         1390 t
ORIGIN      
        1 aacaagaaaa ataaccccaa aagtgcgcag acaatagctg cgaaggaatt caggttttag
       61 tgtggaatgc actggataag aattcatcat gtaataaaaa tgttcatatc gggcatatat
      121 gatccgccgt tgctcctgac tgaacacccc ttcagcatca ataatacgac tgatctcatt
      181 ataacgttga tcccagaatt tttgatgcat ttcaaaattg gtactcttcc gcttgtgtgc
      241 cggattctcg ccttccttaa acaaccccaa aacagtacaa atatcataca acatatttgt
      301 aaatgtgcgc atatgctgtg ctctccggtt ggtttgcatt ttatcgcaga ggacattctg
      361 agcttaacgt atcaaactga cacaacgaca tgcaatgcat atggagaaca atatgtcaaa
      421 tcaaaccgga cagctttaca cgccttcacg catcctgcgt atggctgaac ttacccgact
      481 gctgggtata tcaagatcta ctatttacgt gaaaataaat ccggcctcga aataccacga
      541 ccctaccttt cccaaaccaa taaggcttgg tagtgcatcc gtaggctggc gggcatctgc
      601 catcgatgag tggctgatgt tacatacggc accagcctgg tctgaacctg aaaacaaact
      661 aaaggaactt aatgatgacg acaaaactat cactccctga acttgacaca actaaactgc
      721 ctgacaggtg ccaggcatta cttgatgaaa tgcatgaaga aacaggaata cgccgtgaaa
      781 tacttctgtc tgttatgctg acggtcatgg ctgcctcggt ccaggatacg catgaagttg
      841 aactttccgg agggcaacgt accagccttc agatatttat gtgtctttca tcagcatcag
      901 gcagtggaaa aacctctgcc tgcgcaaaat taatcgctcc tgttcatgaa acagaagaag
      961 aactacatca ggcatatatc gacgataaaa aaaactatga ccgaatgatg gaaatgtgga
     1021 caacagataa aaaaatcctg gagcggagat ataaaaagga aattgaaaga tccccggaaa
     1081 atgctgctgc tgcacgagca gcacttgaaa agtgtatagc aaataaaccc gaacctcccg
     1141 tacaacaggt tctcatcgtg aatgacgcga caccagaagg gatagctttg aaactcagcc
     1201 agtcaccctc tcttctgttg ctgtctgatg aaggaggtac tattctggat aagcgtttcg
     1261 aacgcaaatc ggcactgtac aacacattat ggagtgggca accagtcaac gttgaacggg
     1321 catccagacc cgggttccgg ctgaatgatg tccgtctcac tatacttata ctgactcaac
     1381 cggtaatatt taataagttt tttactctca ctggcgatca gatccggggc aatggctttc
     1441 tggcccggtt gctattttgc gaacccggag caattaaaat aatgacgaca gaaccgcata
     1501 ctgccactcc tgttgtgacg caacaatgtg caagctgttt tcgtaagaaa agttttggca
     1561 gtcagatcag agactctctg agagcatcca gagaaagacg tgcaaaaagt aaagagcgca
     1621 tctgcatgac attatcaaat gcagcatccc gcgcactgga gcgctttcac aaagaaaata
     1681 tggatactgt cagacagaat cctcatatgg gtacatttga agacattatt gtcagaaaaa
     1741 gagagcaggt tgtcaggata gcagcacttc tggaactgga gaaagatcca gactcgacag
     1801 taataacgct ggaaagtact aatagcgcca tttatctcat tgaattttat ttcaagcatc
     1861 ttatatataa acttgaatcc cttcgggaaa tatccccggc tgagaaactt gataaatggc
     1921 ttcagaaaag aattatcaca acagcaggtt acatattcca gaaaagttat attttacaat
     1981 atgccccata tgctctcagg aaaaaatgtg ttctggatga agcacttgac atactggcgg
     2041 aacaaaggaa aataaggatt gatgataacc tggttgttta tataggaaac acgattacac
     2101 catccgaact ggcaaaaaaa ctaaatattc ctgcattcga tgccggagtt ttcatttgtg
     2161 atcatcaaaa catactgaag tatcaccgaa acagactata gatactatcg gacaaaaaat
     2221 aaattatcac aagtgtatta ttcgttataa taatgaataa tcgttatctg agtgcaatgc
     2281 caggttttca ttacgaactg gcattgcatt tatttttcaa aaaagaaagc agtaaccatt
     2341 tggctactct gagtatgcca gtccatgaaa ctaagcgaat tccttagtgt ttttagcaag
     2401 ttgagacatc acataatccg cccatgcctg catcatcggt acacgctgct ccagtagatc
     2461 agtacgatga tatgccgcct caaccttatt tttcagcgta tgagcgagcg ccctttccgc
     2521 cagatcccgc gaatacccct gttcactaca ccagtccctg aatgttgagc gaaaaccatg
     2581 tgccgtggca actcgccccg gaatgtcact gacggctttc tttttacgca gaaaacttgt
     2641 caacaccata tcggaaagga tctgctgctt tctgggtgaa gggaacacca gttcatcatg
     2701 caggccacgt atattttcca gaatgtaaat agcctgccgg gataaaggaa cacgatgctg
     2761 tagcctagct ttcattcttt ctgcaggtat agtccatacc cgcttatgaa aatcaatttc
     2821 agcccagcgc attcccctgg cttcgcccga gcgagttgct gtaagtatca ccattaataa
     2881 cagtgcgcgg gtaacattat aaggttcatc actatacaca ctggtcgcca caaaaagcgg
     2941 taactgcctc cagggcattg cgggttggtg ttcatcacgt cctcttgtct gctgaggaag
     3001 caaatggtca accacatcaa caggatttgc cacacaaaaa ccgtgcgccc atccccactg
     3061 cataacaaca tgaatgcgct gtttaacccg gcttgccgtt tctgacaagg ttaaccagac
     3121 tggacgcagt gtttctgcca cgtccgcagc cgtaatcgaa tccagcgttt ttgctcccag
     3181 ttgaggaaac gcgtaattct caagcgtcga taaccactgc cttacatgct ttggattctc
     3241 ccatccagga gacagttctg catgtacacg cctggctgca tcggcaaatg ttgggataac
     3301 gactttctca gattcagcct ttttaatctc cagaggatca tcacctgcag caagttgctc
     3361 tcgcattatc cgggcagtac gtgcagcttc agcaatactg acctctgggt aagttcccaa
     3421 tccagcatta cgtctttttt gtgtcaccgg acttacataa cgaaaaaccc atttcccccg
     3481 cccctttact gaagaaggat gaagggtcag tccggtaatt cccccatggg gcaatggttt
     3541 gtcatcaggt ttgatatgtc ttgctttcgt atccgtcaat actgccatac gctaattccc
     3601 gtcactctgg tatgccatcc agtatgccat tagtccctcg cttcactaag aatacattag
     3661 ataacatcgg acaacaaaaa gtgtaataca ttgtttataa agaaattata taacacccct
     3721 ggattaagtc agatttattt caggcggtcc ccctcaccgc catatttaaa gaagagcgcg
     3781 tacgaaagta cgggcttttt tttcgtatat tgtacacacc gggtgccccc gcccacgtct
     3841 gttagggcga ggggaaattg tgcacatcaa ctgtctggtt actcagtcaa caatcaaaat
     3901 gcgtaagata gtacagaaca tatatgcgtt atctggtcac ttcctgtaac ttcctcactt
     3961 attaaaatgc ccccaacgga aatacatgtc acatgccatc ataaaatcgt tatgccagag
     4021 gaatcgacaa tggaaaaaat agtaaattaa gcacggtaca tagcagagta aaaaataggc
     4081 ttcaaaccaa atccgccatt ccacaagcgt tgccaatacc gacaccagcg taatccacat
     4141 catttcaata acccgcaata tgaataaccg aagccaaagc ggcttggcaa aaaaggtcga
     4201 caacgtattt tcactggaga aaaacagatc gatttcctgc aaaaaaaacc tgcccagaaa
     4261 agcgggccgc ttggagaagc gcagaaaaaa cagattaatc gccaacacta cgccacaaag
     4321 aatgagcaat gcaattatcg atttaaacgt taaccactgc ccctctgtca caaaagaatc
     4381 actgttacgc agcgggctga tagtaagcag gttaacaacc gttgagatgc acaccgaaag
     4441 cagcacatag cgagtcagac ttttatattt tacttttttc gctactgaaa tagcgacctg
     4501 cttgacataa ctggtgcgga tcatccgaaa cagcagaaag atgaccaacg gcccaagtaa
     4561 cgaaatcagg aacagcgcat ataacgaggg tagaagctgg tatagccccc aactcattgc
     4621 actcaacatg attaacgaca gaaatcctgc caccagaggt tgtgtaagaa ataattctat
     4681 tttgtggttc tgctttttct taaaccacga aaaatcgata ttcttcgtga cgcatttata
     4741 agcccagtac gccttgagat caaaaataaa attatacagc accagggcca gaatcccact
     4801 aactacacca atatcctctg atggaaaagt aaaccctgca tttttccaat aaaagcctat
     4861 cagaataagt ccaatagcat aaatgtatat cagatttcgt ttatcctgaa agcgaataaa
     4921 agttaaataa tcaggaatca tttgaatacc tttgcatcac taaatgcttt acgacaatgc
     4981 ataactcgtt gctgatccac gccagcaaaa tcatttaaca tattccccaa atagggctta
     5041 ttacttattt caaggaaagc taccggggtt ggcatattca gaatatgtat cgagagattc
     5101 agtccatttc accgtatgag ttaagtgtaa tgagagattt tctttgataa ttgacggagc
     5161 gacctcgctt tttgctgtta cgttcatcag aacctgatgc tctggcgaag cgatatccag
     5221 tccggcaaga taatcgcgca ttgcctgaac gccgtcttcc atcaaacgcg tgtgccaggc
     5281 accgctcaca ccgagtttaa ccggttcgta tccagcggcc atcagcagcg tggtaaattc
     5341 attcaacgca gcctgcgttc ccccaataac ctgctggcgc ggcgtgttat cacagctaat
     5401 atccagcgca atgcctgatt ccgtaatcat cgtctgcagt tgttcgcggt tgatgccttt
     5461 aaccgc
//
LOCUS       DAZSIO010000208         5398 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZSIO010000208 DAZSIO010000000
VERSION     DAZSIO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.211"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..497
                     /locus_tag="ACHX5T_003702"
     CDS             <1..497
                     /locus_tag="ACHX5T_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HFR8468707.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="ACHX5T_003703"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="ACHX5T_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="ACHX5T_003704"
     CDS             2500..3114
                     /locus_tag="ACHX5T_003704"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR8468708.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="ACHX5T_003705"
     CDS             3114..3443
                     /locus_tag="ACHX5T_003705"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR8468709.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="ACHX5T_003706"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="ACHX5T_003706"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR8468710.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="ACHX5T_003707"
     CDS             4529..4840
                     /locus_tag="ACHX5T_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR8468711.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4824..5373
                     /locus_tag="ACHX5T_003708"
                     /pseudo
     CDS             4824..5373
                     /locus_tag="ACHX5T_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1177 a         1344 c         1425 g         1452 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca
     5101 caaccttcag aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa
     5161 aagcggggct ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac
     5221 ccggcattac caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat
     5281 gctcgtctcg gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg
     5341 tttttggcga ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggca
//
LOCUS       DAZSIO010000209         5374 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZSIO010000209 DAZSIO010000000
VERSION     DAZSIO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.212"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(99..581)
                     /locus_tag="ACHX5T_003709"
     CDS             complement(99..581)
                     /locus_tag="ACHX5T_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468712.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(649..2598)
                     /locus_tag="ACHX5T_003710"
     CDS             complement(649..2598)
                     /locus_tag="ACHX5T_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR8468713.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2683..3018)
                     /locus_tag="ACHX5T_003711"
     CDS             complement(2683..3018)
                     /locus_tag="ACHX5T_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR8468714.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(3018..3374)
                     /locus_tag="ACHX5T_003712"
     CDS             complement(3018..3374)
                     /locus_tag="ACHX5T_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR8468715.1"
                     /translation="MAKIAGICFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3374..4870)
                     /locus_tag="ACHX5T_003713"
     CDS             complement(3374..4870)
                     /locus_tag="ACHX5T_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR8468716.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVEKYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4867..5028)
                     /locus_tag="ACHX5T_003714"
     CDS             complement(4867..5028)
                     /locus_tag="ACHX5T_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFR8468717.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(5038..>5374)
                     /locus_tag="ACHX5T_003715"
     CDS             complement(5038..>5374)
                     /locus_tag="ACHX5T_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468718.1"
                     /translation="RDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTLL
                     DLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEH
                     HLEMRFPQN"
BASE COUNT         1284 a         1565 c         1155 g         1370 t
ORIGIN      
        1 cagtaagttg gagtcattac caagaaagag aaggtaaaga aggtaagtcc atactgtctc
       61 cactataaaa aaaccgccaa tttctggcgg cttatatatt aatcatcatt aatatctttc
      121 atattcaatt tgcaattttc gtaggtcact cccgactcgc cattaacatt aactccagct
      181 ctgatattcc cgttttcttt tacatctaca aaaaactggc ctcttggcat cttgaacgca
      241 aatgtaaaac caacagtatc tgaatcaaaa tcatcatctt tatatattgg gatcaaaaat
      301 gtcatcgtcg ctggtgatgc attgtttgca aaatcaaata cacctatact tttgccgaat
      361 tcataagaag cttttctcaa tataatcgca ccgctggcag aaggggatgg gcacgatatc
      421 ctcagttgtc tgaatccatc tgtatccaag tcaacgtcat tatctgcaga tgttttaacg
      481 ttactaataa atcgtgaaac aatctcctca tttgttagta ttttcgcctg agcacagcaa
      541 gaggttaata ttgcaaccgt cattaaaaat ggctttatca caaaacaaac tccattgtta
      601 gcatgttacg cgataatttt gcctcatctt tcaccacaaa aaaagaactc accttttaaa
      661 tggagaatac ccaacatcat gggtgatttt cataaaggga tcgcctgttt tcggcaaatc
      721 gataacgcgc attccaggcg gcgcgttgtc aaacgtcacc ttaagttcgc tgcgggttgc
      781 cggtgaagat gcaaggctta acagctgatt cctttccagt gatacattcg gggtgtagcg
      841 tttatcggga gataaatacc catcaccacg tggcatctgc catccggtca tgtcatagac
      901 aaaatcatgg aactgtttgc cccactcgtc gaacttatca ttcagaccaa atccactgtt
      961 cagtgcatca gccacaaaat ttttaacaag ccagggatgt tctttttcaa actcctgcgc
     1021 catcatgccc agttcaagca atccaccaat cagactgatc tttccgaggc cctttaattt
     1081 cagccctgtt tttcctgccg ccgttgtcca ttgattctgg gcgactgttg ccgcacgaat
     1141 acttgccacc gccttcacac caatatacag ggcagaaacc gttgctattg tttttactgc
     1201 ctgttcccat ccccccattg ctctgacaac ctgatcaacc tcctgccaga cttttttcac
     1261 aaccggaccg accgtttccc agttatcaat tatgagatat gcaccaccga cgagaagcgc
     1321 aattaatccc tttgccggcg tcatattcat cacgctaccc atgatttttg ttatgcgtgt
     1381 caatgtgcca atcgccacgc ccatcgtcag taatgccgca ccagttttcg cgatggtttt
     1441 tacgacctca ggattttctc tgacaaacgt gcgcacttct tccagaaaag gcttcatctc
     1501 ttttatgcct tcattgagtg aaggcaggaa ggtttccccc agcgtggaag aaatcgcatt
     1561 gatctggttc tgcagcagca acaactgatt ttctgtcgtc gcggcacggg cggcatattc
     1621 tttctgcatt gagccgccat actgctgcgc atccgccaca cgattaaagt tggttcgcaa
     1681 caaatccaga ttcgtgagca gcggcgcaat ggcccccagg gattccttcc cgaacagcgc
     1741 tttcagcaca gcagcctgtt tttctttcgg cacttttgcc agagaatcca gtacgtgcag
     1801 catggccccg cgggcgtctt tctgcatatc cgccgccagt tttttcgggc taatgcgcaa
     1861 cgagcggagc acttttttct gcgatttggt ggcagaatcc ctcgctgtca gcgacaacat
     1921 aaaattcttt atccccgtcg cagcaatttc tgactccacc cccattccgg caatggttgc
     1981 ccccattgcg gcaatctctc cggaggccac acccgcaaca ctgcctaaag ggccaatacg
     2041 ggtcacaaca tcagaaatct ttttcgcact tgcaggaccg gtattgccaa gatagttaat
     2101 cttatccgca agccctgcca cctctccctg tgtcagttta aaggcagtgc gccactgcgc
     2161 catcatctga ccggattctt ctgccgtggt gtcaaaggcc acgcccatct tcacggcatc
     2221 gtcagtaaac tgcatcagct cgtcacgggc gataccagcc tgaccacccg ccgccacaat
     2281 ttcggcgata ccttccgccg acatgggcag ttctgttgac aggtcgcgca cctgctccgt
     2341 cattgcctta aacgcttccg gcgtatccag accatccacc actttccgga catccgccat
     2401 ttttgattca agggcaatgg cagatttgac cgggagcgcc agcgccccca gtactgcggt
     2461 tccggcaccc gctgcgccca gtgacaggct ggcaaactcc tttttaaaac ctttcagctg
     2521 gcgctgcatt cccttcagcg gtgccgatac cttatccacg gcagtgatga tcgccttcag
     2581 ctgaaaactg tcagccatgc ttcatctcct cgttaatgcg gacggcctcg gcttccagtt
     2641 ctgtaaactg cgaaacagcc acccgtcgca gctccagtgg attcagtttc cagaaccagg
     2701 caacattgta gagtcgtttc cggaggtgct tcccgtctcc gactgggtaa aaaaacgcag
     2761 gatctgcatg ctggtcttaa aaatatccag ttttgccatc tgcgccgccg atgaacacgg
     2821 gatccccgcc agcaacggga tatatttcag tgccacctgg ctgtccagtt taatactgcc
     2881 ttcaccggaa ataatgaaag gaaaacccag cgcctcgatt tcgtcatacg tgggctcgcg
     2941 caactccagc acatgcagca tttcgttatg cgctgtcacc ggttttttta aaactatttc
     3001 tgtaacgttc attgccatta ctgataccct ccctcttcgc cgtggaattc aagatctgcc
     3061 gtgccttctt cggcattatg attagcctct ccgtgcagcc atgccgccga aagcacatac
     3121 accatgccgt ttgccagctc agcggtaatc gtcatctggt ctgaggtggt aattttgtcg
     3181 accggaaagt ttttgggcac tttaaacgtg cccttaacgt aaggtgaccg ccaggtctcc
     3241 ttgtaatcca catcccctgc catgccgacg atgtcatccc ggacattggt gttcatcggc
     3301 acttcaatgc cacctgtcag cgataactgc tgaccatcca ctttaaaaaa acagattccg
     3361 gcgatctttg ccattatgct gactcctctg aatactgaag acggaactgg ttaaccaccg
     3421 caaagacacg taactgatta acataatccg gcgggaacag cgtattcagt cggttcgggt
     3481 tatctgcatc acgctcaact atcagatact gtttaaacag atcgtatttt tccacaatac
     3541 cggcacgctc catctgacga tatgtcgcca gaagttcccc tttgataacg gcaggagtga
     3601 caatcgcctg ccccggacca aaacgggtac catcatttgc cagcttgtga cgtccgtact
     3661 tgcttgtgat gaccgatttc agtttgcgaa gaacatatgc actggtatgc agagtttcac
     3721 tgtccagata gctgttgtct gccacgccat acgcattctt tttgtaggtg gttacggaac
     3781 gctggatctg caacgtgccg ccttccacat aagccgtcgc cacgccgtga gataaaaggg
     3841 tctgctgctc tgtcatgatg aatcgcttac ctttcggtgc cggaagcatc cccaccagct
     3901 cccccgtctg tgtcggacgg gcaggatcat tccggataaa taccgcttca cgggcaaggc
     3961 gactggcaac cagttcatcg acaggcgact gggtttcttt ttcgtaaccg gcaagcgtga
     4021 tatgttgctg attatgcata tctccggcac caaccagctc tgacagcgtt cccagttttg
     4081 cggtatagac atgcccgtat aactggcgcg cataactcca gcgaccgctg ctgtcattca
     4141 tttcggtcat catcatattg atggaggcgg catcgttgaa cggcagaccg ataaagtcga
     4201 atgcctcatc gcccatagca gcaacagcgg cggtaagatc aggcgctccg ctaccggcag
     4261 caccgacttc cgtcaccacc cgaagccctg ctggcaaaat ctcaccacca ccagaaccat
     4321 aataattcag acagaccggt aactcattac catacagccc cttatgacgg gcagtcagcg
     4381 tcaccacgcc tgcatcagat gacgccgtaa acggcagggt gataaccccg tttaccgctt
     4441 ccttaatcgc ggtggcaacc gcagtggcat catcgccatt caccacaggc acctgtacac
     4501 gggagcgccc gacatacaga ctcagggtgc cgctttcctc tgcttctccg gcaaccgtca
     4561 ccctgaccgt cgccgccgcc cctctggctt ccggtactgc aataacatac agttcaccga
     4621 aaggatctgt ctgacggtag acttccacca tacgcgccag ctggctcccc gccccacaaa
     4681 tctggcgggc ataatctgcc gatggcatca gcaccaggct gttaacctca atggctgcat
     4741 cgttgctggc atgcccgatc agtaatgcag gcgcgctggt caccgctgta tttgccgcag
     4801 aattgttcat ctcggcgtag aacaacggca ccagcgtatt cgacggaaca gtattaaaac
     4861 ttatcgtcat gattttttag ccttatcctg tttaacacgt accacatcac ccgccgctat
     4921 acggcggaac cagtagctgc tttcttccac atttcgccct tcggaaggca aaaggtctcc
     4981 acgggcaggg tcaggaaccg accgcccttt caggggtttc acaaacatga gggactctta
     5041 attctgaggg aaacgcattt ccagatggtg ctcaatgtca ccatccgggc cagtaccagg
     5101 atcgatgtaa tccacatcaa tactcagcag ggataaatcc ggcagggcat tcacgtcctc
     5161 tgcctgtcgc gtgtcttctt ccgtgatttc atacttcgcc gtaaaatcaa actggtaata
     5221 cagttcgtaa cggttcagat ccagcagggt gccaccgaca taaacaatct cgcccccttg
     5281 cgggtccggc atccatccaa gcagggcttt ccagagttct ctccggacat catgaacggc
     5341 gtcataggct gcccactgcc ctttttcatc acgc
//
LOCUS       DAZSIO010000210         5339 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZSIO010000210 DAZSIO010000000
VERSION     DAZSIO010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.213"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACHX5T_003716"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACHX5T_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR8468719.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="ACHX5T_003717"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="ACHX5T_003717"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR8468720.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="yfiH"
                     /locus_tag="ACHX5T_003718"
     CDS             1884..2615
                     /gene="yfiH"
                     /locus_tag="ACHX5T_003718"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR8468721.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="ACHX5T_003719"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="ACHX5T_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR8468722.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1290 c         1457 g         1282 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcggg
//
LOCUS       DAZSIO010000211         5277 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZSIO010000211 DAZSIO010000000
VERSION     DAZSIO010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.214"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(58..1341)
                     /gene="aroA"
                     /locus_tag="ACHX5T_003720"
     CDS             complement(58..1341)
                     /gene="aroA"
                     /locus_tag="ACHX5T_003720"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR8468723.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1412..2500)
                     /gene="serC"
                     /locus_tag="ACHX5T_003721"
     CDS             complement(1412..2500)
                     /gene="serC"
                     /locus_tag="ACHX5T_003721"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR8468724.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2699..3325)
                     /locus_tag="ACHX5T_003722"
                     /pseudo
     CDS             complement(2699..3325)
                     /locus_tag="ACHX5T_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3455..5215
                     /gene="ycaO"
                     /locus_tag="ACHX5T_003723"
     CDS             3455..5215
                     /gene="ycaO"
                     /locus_tag="ACHX5T_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR8468725.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1346 a         1358 c         1263 g         1310 t
ORIGIN      
        1 acaatgtgcc gacgttttga taaagaaaga tttggctatt tattgcccgt tgttcattca
       61 ggctgcctgg ctaatccgcg ccagctgctc gaaataatcc ggaaatgttt tggccgtgca
      121 tttgggatca agaatcgtca ctggtgtatc tgacaacgcc accagcgaga aacacatcgc
      181 catccggtga tcattgtatg tcgcgatctc ggcaaagttc agtttttccg gaggagtgat
      241 acgaatgtaa tcgtgcccct cttccacttc cgcgccgact ttacgcagtt ctgttgccat
      301 cgcaaacagg cgatcggtct ctttaacacg ccagttatag atattgcgca gcgtggtggt
      361 gccttttgca aataacgccg ccgtggcaat ggtcatcgcc gcatcaggaa tatggttcat
      421 atccatatca atagcgttca gttcaccacg cgtgcaggaa atataatcat cgccccagca
      481 aatggtcgcg cccatttttt ccagcacatc agcaaagcga atatcaccct gcatactgtt
      541 acgtccaata ccggtcactt ttacagtgcc gcctttgatt gctgctgctg ccaggaagta
      601 agaagccgaa gatgcatcgc cttcgaccaa ataagtaccc ggagactgat aagactgccc
      661 gccttttacg acaaattgtt gatagtgctg attttcaatt tcaacaccaa acgtcttcat
      721 cagattgagt gtgatgtcga tataaggttt agaaaccaga tcgcctttaa tacgaatcac
      781 cgtatcttcc ggcgcaagag gcgcagtcat taacagtgcg gtgaggaatt ggctggaaac
      841 ggagccatca acgtcaacgt tgccgccagt aaagccgccc tgtaaacgca acggcggata
      901 attttcttgt tccaggtaag tgatcttcgc cccgcccagg cgcagcgcat ccaccagatg
      961 accaatcggg cgctctttca tacgcggctc accggtcagc acaatatcat tgctatccag
     1021 acaaagagct gccgccagcg gacgcattgc cgttccggcg ttaccgagga acaactccag
     1081 ggcaccttct gcgtgtaatg gaccgccgtt accgataatt tcgcaacgcg tacgatcggc
     1141 tgaaagcgta tagcttaacc ctaacgctgt taatgcattc agcatatggc gcacgtcgtc
     1201 gctatccagc agattggtta atactgtttt gccgtgtgct aatgccgcaa gcaataaagc
     1261 gcggttagaa acgctcttgg aaccgggcag attaatagtg ccatcgacac gagcgatggg
     1321 ttgtaacgtc agggattcca tgaactcaac tctctacaac agaaataaaa accccacagg
     1381 ctggctgtgg ggattaagca aaatttcggc attaaccgtg acggcgttcg aactcaacca
     1441 tgaagtctgt cagcgcttta acgccttcca gcggcatggc attataaata gacgcgcgca
     1501 ttccgccgac cacacggtgt cctttcagtg catgaaggcc tgcagcaaaa gactcttcaa
     1561 ggaacaattt gtcaagcgca ctgtccgcca actggaacgg cacgttcatc cgcgaacggt
     1621 tagctttcgc cacgtcattg cggtagaaat cgctgttatc aatcacccca tatagcagtt
     1681 ctgctttttg ctgattgatt ttatccattt cagctacacc gccgttcgct ttcagccatt
     1741 taaagaccag accagataga taccaggcaa atgtcggcgg cgtgttaaac atggagccgt
     1801 tatcgttgag gatggaataa tccagaatcg acggacacgc gatattcgct ttgcccagca
     1861 aatcttcacg aacgatgacg attgtcaggc cagccgggcc gatatttttc tgcgcgccag
     1921 cgtaaattac accataacgg ctgacgtcaa tcggacggga aagaatggtt gaagagaagt
     1981 cggcggcgac caccacatct ttgccgaagt ctggcgtctc gtcgatggcg ataccgtcga
     2041 tggtttcatt cgggcagtaa tgcatataag ccgcattatc agagagttgc cattcacgca
     2101 ttggcttaac cgcgcgcaga ccatcaacag tcactttggc gtcaaagaca ttaggcgtgc
     2161 agtatttttt cgcttcttta atggcacttg ccgcccagta accggcatca acataatctg
     2221 cggtggtttt atcaccgaga atattcagcg gtaccgcagc aaactgaccg cgaccaccgc
     2281 catggcagaa taataccttg tagttggagg ggacattaag aagatcgcga aaatccttct
     2341 cggcttcctc tgcaacctga atgaactctt tgccacggtg actcacttcc atcaccgacg
     2401 taccaagacc gttccagtcg cgcagttcct gttgagcctg tttaagcacc tctgccggta
     2461 gcattgccgg accagaacta aaattgaaga tttgagccat ttcccctcac cacgttgcgt
     2521 tgctattaac ccgcggtcaa tcgattgcga ccacaggtaa tacattattc ctgtggctat
     2581 cggttttatc attcagtgac acacatcgca atgagtaaaa ccgttgaggg caaaaatgtg
     2641 gcttgcatca cacaaaagaa tctctcggat tattagcaaa aagccgatgt ttttactgtc
     2701 aggtcacccg ttttgcccga cttgccttcg tccattctgg atttgcacag cgcggacata
     2761 attgtttttc ccccgcgttc atatgaatga tttcactaca acgaatgcag gcatagtccg
     2821 ccgactccgg caccactttg taacgctgcg tacaatcact gggtaaaata agtaaacccg
     2881 gtttcacctt gtcatcttca aagaaataga cactgatttg cccgttggtt tcgagaatcg
     2941 ccagacgtac ctgccccagc tgctccacgc cacgcaatcg tagctccata aagaactcaa
     3001 attccgtcat gttggagtta ttgagtttcg accaggccag ctcgccatca cattaccagg
     3061 cggtataaca acgccagagt gataaagacg ataagtaccg gcaccatcgg cacatcatca
     3121 taaaacgcca catctcccgc cgctgaaccc agcgtgagaa tgattaacac ttcaaacagc
     3181 gacatctgtc gcacaccgcg tcttccggtc attttgagga acaaaaaacc agtacaaagg
     3241 tataaagact acgtagtgca acttcgccaa ggaaatcaaa aggcacttta tcaaatgcca
     3301 tacggtggag atcgaacgcc ttcattttta tgctcttaac gtggtttatc cgctaaaagc
     3361 atagtcaaca gttcatcggc atggcggcgg tgatagcgcc tttgagataa aacccgtatt
     3421 attgcgcgct ttccgtacga ctaaagtgat tttcatgacg caaacattta tccccggcaa
     3481 agatgccgct ctggaagatt ccatcgctcg cttccagcaa aaactttcag acctcggctt
     3541 tcagattgaa gaggcctcct ggctgaatcc cgtgcctaac gtctggtctg tacatattcg
     3601 cgacaaagag tgcgcactgt gttttaccaa cggtaaaggc gcaaccaaga aagcggcgct
     3661 ggcttctgca ctcggtgaat atttcgagcg tctctcaacc aactactttt ttgctgattt
     3721 ctggctgggc gaaaccatcg ccaacggtcc atttgtgcat tatcccaacg aaaaatggtt
     3781 cccactgagc gaaaatgacg atgtgccaga agggctgctc gatgaccgtc tgcgcgcatt
     3841 ttacgatccg gagaatgaat tgaccggcag tatgctgatt gacctacaat ccggtaacga
     3901 agatcgtggt atttgcggtc tgccgtttac gcgccagtcc gacaatcaga ccgtttatat
     3961 tccgatgaat atcattggta acctgtacgt ttctaacggt atgtccgctg gcaatacccg
     4021 caacgaagca cgcgttcagg ggttgtccga agttttcgaa cgctacgtga aaaaccgcat
     4081 tattgctgaa agcatcagct tgccggaaat cccggcagac gtgctggcgc gttacccagc
     4141 ggtggttgaa gcgatcgaaa cactggaaac agaaggtttc ccgatcttcg catatgatgg
     4201 ttcccttggc ggccagtatc cggtgatttg cgtggtactg tttaatcctg caaacggcac
     4261 ctgctttgcc tctttcggtg cgcatcctga ttttggcgta gcactggaac gtaccgtgac
     4321 cgagctgctg caaggtcgtg gcctgaaaga tttggatgtg tttactccgc caaccttcga
     4381 tgatgaagaa gtcgctgaac ataccaacct cgaaacgcac tttatcgatt ccagcggttt
     4441 aatctcctgg gacctgttca agcaggatgc cgattatccg tttgtggact ggaatttctc
     4501 cggcaccacg gaagaagagt ttgctacgct gatggctatc ttcaacaaag aagataaaga
     4561 agtttatatt gccgattacg agcatctggg cgtttatgct tgccgtatta tcgtgcctgg
     4621 catgtccgat atttatccgg ctgaagatct gtggctcgcg aataacagta tgggcagcca
     4681 tttacgtgaa acgattcttt cgctaccagg cagcgagtgg gaaaaagaag attacctgaa
     4741 cctcatcgag caactggatg aagaaggttt tgatgacttt acccgcgtgc gtgagctgtt
     4801 gggtctggcg accgggtcgg ataacggttg gtacaccctg cgtatcggtg aattaaaagc
     4861 catgctggcg ctggctggtg gcgatctgga acaggctctg gtctggaccg aatggacgat
     4921 ggagtttaac tcatcagtat ttagtccgga acgcgccaac tattatcgct gcctgcaaac
     4981 gttgttatta ctggcacagg aagaagatcg ccagccgctg caatatctga atgcgtttgt
     5041 tcgcatgtac ggcgcagatg ccgtagaagc cgccagtgcg gcaatgagcg gcgaagcggc
     5101 gttttacggc ctgcaaccgg tagatagcga tctgcacgcg tttgctgcac atcagtcgtt
     5161 gttgaaggcc tacgaaaagc tgcagcgcgc caaagcagca ttctgggcaa aataaaatca
     5221 aatagcctac gcaatgtagg cttaatgatt agtctgagtt atattacggg gcgtttt
//
LOCUS       DAZSIO010000212         5272 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZSIO010000212 DAZSIO010000000
VERSION     DAZSIO010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.215"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..171
                     /locus_tag="ACHX5T_003724"
     CDS             <1..171
                     /locus_tag="ACHX5T_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8468726.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..1628
                     /gene="celF"
                     /locus_tag="ACHX5T_003725"
                     /gene_synonym="chbF"
     CDS             276..1628
                     /gene="celF"
                     /locus_tag="ACHX5T_003725"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR8468727.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1641..2399
                     /gene="chbG"
                     /locus_tag="ACHX5T_003726"
     CDS             1641..2399
                     /gene="chbG"
                     /locus_tag="ACHX5T_003726"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR8468728.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2446..4707)
                     /gene="katE"
                     /locus_tag="ACHX5T_003727"
     CDS             complement(2446..4707)
                     /gene="katE"
                     /locus_tag="ACHX5T_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR8468729.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4890..5153
                     /gene="cedA"
                     /locus_tag="ACHX5T_003728"
     CDS             4890..5153
                     /gene="cedA"
                     /locus_tag="ACHX5T_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR8468730.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT         1298 a         1184 c         1391 g         1399 t
ORIGIN      
        1 atccgtatta attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg
      421 atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt
      481 ataaaacgct ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc
      541 gcgttggcca attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc
      601 ttggtcagga aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga
      661 tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca
      721 ctaacccggc gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta
      781 tcggcgtgtg taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga
      841 aagacagcga tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaagg
      901 atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc
      961 agttaaaagt atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc
     1021 gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc
     1081 tggctattga aatgggcgaa tactacaaag gcggcgcacg agcgcaggta gtacagaaag
     1141 tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac
     1201 tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatct
     1261 acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata
     1321 ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac
     1381 atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct
     1441 tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc
     1501 taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga
     1561 ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag
     1621 cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct
     1681 taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga
     1741 cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac
     1801 cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg acagctatgc
     1861 cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg
     1921 ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc
     1981 tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga
     2041 ttttcccgat tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc
     2101 agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta
     2161 gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa
     2221 gccatcgggg tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta
     2281 tccgtcagag tgcttactgt ttcccacggt taacggagct ggaagtgctg acatcagcgt
     2341 cgttgaaata tgcaattgct gagcgtggtt atcggttggg tagttatctt gatgtgtaag
     2401 taaggtggta aggtcactcc ccaggggacg tgaccttatc catatttacg ccggaatttt
     2461 gtcaatctta ggaatgcgtg accacacgcg gtgttctgcc atcagcgtta acagttcatc
     2521 cataaaacta ccgtcggtgc tgtcagcttc cacaatccct tcttcaccct ggtcagctac
     2581 cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt
     2641 gtaggcttcc atcagatagt agttggcatc gccgttgtca gcgatatccg cgatattgcc
     2701 gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat
     2761 aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga gcagtttggc
     2821 atgaacgcct ttggccttca tggccttgag aatggccaga aggtctgccg atctcacttc
     2881 atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa
     2941 acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga tattcagctg
     3001 gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag tgagatcaat
     3061 atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt tgcttaactc
     3121 aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta gccagaacag
     3181 acgcggatgg gaataatatt cgccaaacga tgggctgcgc tcgcgaactt tattgccttc
     3241 cacgcgctct tggtatgatt caaaaccgcc gcgtttcggc cccggtggtg tttcgcgcgg
     3301 ccagttatcg ttgatcgagt tcggttcata attcgccggg ttagtgtcga tccccatacg
     3361 atgcatgccg tcacgctgga aattatggta agggcaggtc gggcggttaa tcggaatctc
     3421 atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga acaaacgtcc
     3481 ctgcaacagg ggatcgttgg tgaagtccag accaggaaca atatgacctg gatggaatgc
     3541 tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca ttttgccgac
     3601 acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa gatcgaagtc
     3661 gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt ccggaaaatc
     3721 gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac gtccggtgag
     3781 tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc agtggaaacg
     3841 tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac cgaagccttc
     3901 catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca cgttgtgcag
     3961 agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt gcccttgtgg
     4021 aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc
     4081 ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct cttcagtata
     4141 gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag cgccaccctg
     4201 aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga ggaaatccgc
     4261 tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg ctgagccgcg
     4321 tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag
     4381 aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt tttgatcatc
     4441 ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt tgcgtacgtc
     4501 ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc caggggcggt
     4561 aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt cctcaggtgc
     4621 cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg
     4681 tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt actgaaaggg
     4741 ccgctgtttt ggtcaaaaaa ctctctaact atagaccacc agccagatcc cgcttcggag
     4801 actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg
     4861 gctaaactac agtttttcta atttttaata tgcgtttagt gaagccagta atgaagaaac
     4921 cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa cccgcgccgc
     4981 cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg
     5041 ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat
     5101 ctgcccaacg ttgggcaaat cagatccgcc aggaagggga agtgactgag taaggattac
     5161 atacctcccg gttttgccgg gagttacagg acctgaaatt ctgcatttcg ttgctacatt
     5221 cattcaaacc acctgtaaca aaaagctgcc tttgacgagc tattgctata aa
//
LOCUS       DAZSIO010000213         5250 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZSIO010000213 DAZSIO010000000
VERSION     DAZSIO010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.216"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..227
                     /locus_tag="ACHX5T_003729"
     CDS             <1..227
                     /locus_tag="ACHX5T_003729"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468731.1"
                     /translation="KKCTVVNMSDINFGIFFIIAFGFITVGSFFIVLIRFLERAETIL
                     IGHLDLKSKAIIHGLIILFIYWTLKFFAES"
     gene            339..884
                     /gene="ddp1"
                     /locus_tag="ACHX5T_003730"
     CDS             339..884
                     /gene="ddp1"
                     /locus_tag="ACHX5T_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 1"
                     /protein_id="HFR8468732.1"
                     /translation="MKQIKFRLDDAKHHDLLICLKSLYPDEPGLTVSKGMKLLANALL
                     KSKKTNQIQNDYADENNFIKTTLYLTGKQRADIEKAANRHGWTLSRECRYRIQTTLNN
                     RLDFFDQELLMMNRCRNSIDKIGRNFHYIIVNDQTRVLDKDGFYQDAERLKTEIFSLK
                     NQFENYIMLCKGRTVSNKVDV"
     gene            1992..2723
                     /locus_tag="ACHX5T_003731"
     CDS             1992..2723
                     /locus_tag="ACHX5T_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR8468733.1"
                     /translation="MTKKNVDQPMTMTVSLRGKNVVMSNAISRAAQSLSLAEKRIIFA
                     AIAQMGGKYAPVRITAQEYANTFGMPLKQAYSQLKEASENFFNRYIRFKLNEVSEIDV
                     DVWKIRWLGAYKYNDGEANVLLHFTPQVMPYLCELEDNFTKYQLSQACALRSVHSWRL
                     LELFEQMNTNKENNGWLSISLEDFHHAMESPDSYKTTFGLLRKYMIEPAVKELTEKDH
                     WMIDWKPIKKGRKVVRLEFWFRKEN"
     gene            3145..3903
                     /locus_tag="ACHX5T_003732"
     CDS             3145..3903
                     /locus_tag="ACHX5T_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFR8468734.1"
                     /translation="MKKRIISAVLASLSVISVAQAGIPVAIDANPEWAVEAGRWTERL
                     KQWAETVKHYENQINAYKQELLSKTGIRDVQGLVQSAQSVSQELMQIYDQGNAFIDDY
                     IKNPESALSEQAKSLLSDYRVTDTCQNLGYSGDQVRGCEATFLSQLASVEYGNKLESK
                     LRQDNQTMKDLIEQVKNAKDTKATQDATNAVALEQLKFEKLKFQYQMYIDKQKQLSEY
                     KKEMAKASFRHEQINAKPIDYREAFSTQDFDLIK"
     gene            3913..4182
                     /locus_tag="ACHX5T_003733"
     CDS             3913..4182
                     /locus_tag="ACHX5T_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HFR8468735.1"
                     /translation="MKYIVLVFLASLSFILTGCNEDTKSVDWWTNHPKEAVDKYKECK
                     KTGSDSDNCKNVRRAGLIIADTYPPMSEIYKQEARELDKKLGVSK"
     gene            4196..>5250
                     /locus_tag="ACHX5T_003734"
     CDS             4196..>5250
                     /locus_tag="ACHX5T_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFR8468736.1"
                     /translation="MATDNTYPVYDGNFFRATHDIILRAVEKSTSGMFDNYINIAYTL
                     GKYGVSLYILWYAFTVLARKQQTPVPDFIWNLCRFSIILMFVKNTGGWLDNANQAIDG
                     LKETLAGGDPWLWMDQLWDKVITVGKKVWHLDTDTIPVVGVAAALLTYAGGILAMIVC
                     AIVFFSAEITLKILSITAPLFIMCLMFGFLRQMFNNWLQLIFSSLLIFMFAALALRAG
                     MWQLNGTLSSTLSSATAPNLFEAGATVLVSGIFMAWIIWQAKTYASQLAGVGVEGAMQ
                     GAAAMGIGAGIFGASRMTRGALGMGRNAGIGAWKGLRRQEGGFGQSPGITGKAANLAG
                     QGVNIGAKKLRQAAIERA"
BASE COUNT         1578 a          915 c         1177 g         1580 t
ORIGIN      
        1 aaaaaaaatg tacggtggtt aatatgagtg atattaactt tggtatcttt tttataatcg
       61 catttggctt cattactgtt ggttcatttt ttattgtgct tatccgtttt cttgaacgtg
      121 cagaaaccat attgattggt catttagatt taaaatctaa agccattatt catgggttaa
      181 ttatattatt tatatactgg acgcttaaat tttttgcaga atcttaatca aagaaccgac
      241 tgtaggtcac cgggcaaacg gcaaaaatgg aaccattttg cggcgtttgc cctatcctgc
      301 atcggagtaa atattcatgt gctgtaggaa gtgcaaatat gaaacaaatc aaattcaggc
      361 ttgatgatgc caagcatcat gacctgctga tctgcctaaa gtctctttat cctgatgaac
      421 caggcttgac tgtatcaaaa ggtatgaaac tgcttgccaa tgcattgctg aaaagcaaaa
      481 aaactaacca gatacaaaat gattatgctg atgaaaacaa tttcatcaaa accacgcttt
      541 acttaacagg taaacaaagg gctgatattg aaaaagcagc taaccgtcat gggtggacat
      601 tatcacggga atgtcgatat aggattcaaa ccacactgaa taataggctt gatttcttcg
      661 accaggaact gctgatgatg aatcgttgcc gtaattcaat tgataagatc ggtcgtaatt
      721 tccattatat cattgttaat gatcagacca gggttcttga taaagatggt ttctatcagg
      781 atgcggaacg tcttaagaca gaaattttta gtcttaaaaa tcagtttgag aattacatta
      841 tgttatgtaa ggggagaaca gtttcaaaca aggtagatgt atgaagtcca cattgataac
      901 taaatctagt ttgtattgtt gaataaacta catgttgctg atagagtctt tgcgagatgt
      961 gaatttatcc ttttgttttt ttctttgctt cggggtcatc cgagtcgaag gagcgtctag
     1021 tagaaacgga actactggca aatttgttac tatttcagtc ttgatatagc tgtaaactgt
     1081 ttatagtata aactttgcgc aataataaaa aatctctctc catagagatg tgatttatta
     1141 cgagcaacta cggttagctt gtgttttgtc acgtctaaac gttagtcagt tccagtaatg
     1201 agatcatcgt tgttattcct gtgcaaacgg cctttgcagg gagctggcgc ggtctcctgc
     1261 ttcctgaaag cccctggtta ggggtatcgg cgcgcctgac ctcctgaaac gctctgtaat
     1321 cgtttttcgc gccttccatg ccctgacggg catttaaaac ctgtttcgtg cgtctgagag
     1381 cttctgagcg cgttttaagg gccaatacga agaaaaccat taccaccacc agaaaatcgc
     1441 cttctggtgc tctggatggc tcacagaacg cattttcgaa cagaacagca acggaatcgc
     1501 cgacaacatg cgagaggtgg taccgctcgc cgcagtcgaa cggcaggagc gtagcgactg
     1561 agtgagcgag gaagcggaag tgagtctgca ggtgagttga gcacatacgc atagcggtta
     1621 aaaaatgagc ggattgtttt taggtattgt tgctctagct taacgtgatc tgtcctcttg
     1681 gtttgagcat ggttatccac agattttggg ggcaaattcc tttttaaacc ctttttatct
     1741 tttcttttta aaaccttttt agacgccaaa actcttttaa aatcatgacg ttacagagtt
     1801 aaaagtaagt tttttcgggt taaaagtaag ttttttcggg ttaaaagtaa gttttttcgg
     1861 gttaaaagta agttttttcg ggcattatcc acagctaact gtaagagtaa aaaatgattt
     1921 tacttttcat tttttgtaag tatgatttac gtacttactc ttgccctgta catcttactt
     1981 ttgagtgaat aatgaccaag aagaatgttg accaaccaat gacgatgact gtcagtttac
     2041 gtggcaaaaa tgttgtcatg agtaatgcta tatctcgtgc cgcgcaaagc ctttctttgg
     2101 ctgaaaagcg catcattttt gcagctattg ctcagatggg tgggaaatat gcgccagtaa
     2161 ggattacagc acaggaatat gctaacacct ttggaatgcc tttaaagcaa gcatattcac
     2221 aattaaaaga ggcttcggaa aatttcttta atagatatat tcgctttaaa ttaaatgaag
     2281 ttagtgagat tgatgttgat gtatggaaga ttagatggtt aggggcgtat aaatataatg
     2341 atggtgaagc taatgttctt ttacatttta cgcctcaagt aatgccatat ttatgcgaac
     2401 tggaagataa ttttacgaag tatcagctat ctcaagcttg cgcattgcgt tcagtacatt
     2461 catggcgttt gttagaactc tttgaacaaa tgaatacgaa caaggaaaat aatggatggt
     2521 tatccatatc gttagaagat tttcatcatg ctatggaatc acccgatagt tataaaacaa
     2581 cgtttggatt acttcgtaag tatatgattg aaccggcggt aaaggagtta acagagaaag
     2641 atcactggat gatcgactgg aaacctatta agaaggggag aaaagtcgtt cgtcttgagt
     2701 tctggtttag gaaagaaaac taatcatatc aatgattaac gctggtaatg agggctgcca
     2761 ccctcactac cagctaacca caacaacctg ttcggaggct gtatggctgg cccaagtata
     2821 tctgaatatc agttaaaaac ccgcaaagaa ttttacgctt atcttgatag taagaggaaa
     2881 gaaggaaact cgagatttag tttatccctt gagaaagacc ctttctcttt ttctttatat
     2941 cttttgttca caagtaatga aggtattcgt aatagtgatt taatccgtcg cttccgaatt
     3001 actagcaggg gcacgaggtt tattgtaagt aactttaaat tctggcttaa ggaatacaat
     3061 cgcggtatgt ttaagtgatg ttagccctct ctttaattaa aggagagggc taattatcag
     3121 gattatattt agtggagatc aataatgaag aaacgcatta tatcggcggt gctcgcttcg
     3181 ctctcggtta tttccgtcgc tcaggcgggg atacctgttg ccattgatgc caatccggaa
     3241 tgggcggttg aagccggacg atggacagaa cgtctgaagc aatgggcaga aacggtgaaa
     3301 cattacgaaa atcagataaa tgcgtacaaa caggagctgc tgtcaaaaac gggtatccgt
     3361 gatgtgcagg gactggtgca gtccgcacag tcagtgagtc aggaactgat gcagatttat
     3421 gatcagggga atgcttttat tgacgattac attaaaaatc ctgaaagtgc gttatcggaa
     3481 caggccaaat cgttattgtc agattacagg gtaacggata cctgccagaa cctgggatat
     3541 tctggtgacc aggtacgggg atgtgaagcg acgttccttt ctcaactggc aagcgtggaa
     3601 tacggtaaca agctggagag caagcttcgt caggacaatc agacaatgaa agaccttatc
     3661 gagcaggtta aaaatgcgaa ggatacgaaa gccacgcagg atgcaacaaa cgccgttgca
     3721 cttgaacaac tgaagtttga gaagctcaaa tttcagtatc aaatgtacat cgataaacaa
     3781 aaacaactgt ctgaatataa aaaggaaatg gcgaaagctt cttttcgtca tgaacaaatt
     3841 aatgcaaaac caattgatta cagagaagca ttttcaacac aagactttga tttgatcaaa
     3901 taaggggttt ttatgaaata tatcgtcttg gtatttttag catctttatc atttattctt
     3961 accggatgta atgaagatac aaaatctgtc gattggtgga ctaatcaccc gaaagaagct
     4021 gtcgataaat ataaagaatg caagaaaaca ggaagtgact cagataattg taaaaacgta
     4081 agacgtgcgg gacttataat cgctgacact tatcctccga tgagtgaaat atacaagcaa
     4141 gaagccagag aacttgataa gaaattaggc gtttcaaaat aaccgagggg gttatatggc
     4201 tacagataat acttatcccg tatatgatgg aaatttcttt agagcaactc atgatataat
     4261 attaagagca gttgagaaat caacatcagg aatgtttgat aattatatca atattgctta
     4321 tacactgggt aaatatggtg tttctttata tatcttatgg tatgcattca ctgtcttagc
     4381 aagaaaacaa caaactcctg ttccagattt tatctggaat ttgtgcaggt tttcgataat
     4441 tttaatgttc gttaaaaata cagggggatg gcttgataac gccaatcaag ctatagatgg
     4501 cttaaaagag actctcgccg gtggtgatcc ttggttatgg atggatcaac tttgggataa
     4561 agtaattact gttggtaaaa aggtttggca tcttgataca gatacaatcc cagttgtagg
     4621 tgttgcagcg gccttgctta cttatgccgg aggcatttta gcaatgatcg tgtgtgcaat
     4681 agtatttttt tcagcagaaa ttacgttaaa aatcctttct attacagcac cgttatttat
     4741 tatgtgtctg atgttcggtt ttcttcgtca gatgtttaat aactggttac agcttatttt
     4801 cagttcatta cttattttta tgtttgcagc actggcgtta cgtgcaggta tgtggcaatt
     4861 gaatggaaca ttaagcagca ctttatcttc agcaacagct cctaatttat ttgaagcagg
     4921 tgcgacagtt cttgtctcag gtatctttat ggcatggatt atctggcagg caaaaaccta
     4981 tgcttcacag cttgctggcg tgggtgttga aggtgccatg cagggcgcag ccgctatggg
     5041 gattggtgct ggcattttcg gtgcatcccg tatgacgcgt ggtgcacttg gtatgggcag
     5101 aaatgccggt attggtgcat ggaaaggtct gcgtcgtcag gaaggcgggt tcggacagtc
     5161 tccgggaata acgggcaaag ccgctaacct ggccggacag ggcgttaata ttggtgccaa
     5221 aaaacttcgt caggccgcta ttgagagagc
//
LOCUS       DAZSIO010000214         5197 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZSIO010000214 DAZSIO010000000
VERSION     DAZSIO010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.217"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..244
                     /locus_tag="ACHX5T_003735"
     CDS             <1..244
                     /locus_tag="ACHX5T_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468737.1"
                     /translation="EMLSFLTSEEREALTNMLARKGVETVLRLLDKETQELLQLEGRK
                     RLAALMLVDMPDEQVREILEEIEASILSESVNAKAG"
     gene            493..1437
                     /gene="tauA"
                     /locus_tag="ACHX5T_003736"
                     /pseudo
     CDS             493..1437
                     /gene="tauA"
                     /locus_tag="ACHX5T_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
     gene            1450..2217
                     /gene="tauB"
                     /locus_tag="ACHX5T_003737"
     CDS             1450..2217
                     /gene="tauB"
                     /locus_tag="ACHX5T_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR8468738.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            2214..3041
                     /gene="tauC"
                     /locus_tag="ACHX5T_003738"
     CDS             2214..3041
                     /gene="tauC"
                     /locus_tag="ACHX5T_003738"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFR8468739.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            3038..3889
                     /gene="tauD"
                     /locus_tag="ACHX5T_003739"
     CDS             3038..3889
                     /gene="tauD"
                     /locus_tag="ACHX5T_003739"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR8468740.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACHX5T_003740"
     CDS             complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACHX5T_003740"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR8468741.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1187 a         1307 c         1478 g         1225 t
ORIGIN      
        1 agaaatgttg tcgtttctta ccagtgagga acgagaagcc cttacaaaca tgctcgcaag
       61 gaaaggtgtt gagactgtgc tacgcttgtt agataaggaa actcaagagc tactgcaact
      121 agagggccgt aaacgcctgg cagcgttgat gctcgttgat atgcccgacg agcaagtcag
      181 agagattttg gaagagattg aagctagtat tctctcagag agtgtaaacg cgaaggcggg
      241 ttaacttcat tgacctgcct ttagggtgcc tttaaaattc gtttttaaag gctacgatga
      301 catttttgaa gagtcttaaa agaattaaaa tacacggcgc gcctatcttg aatgttttat
      361 cgatatcgaa gcattgagca tttttaactc gttttatctt gaatgcaatc ggcgcataac
      421 ttattgaatc ccatcaaatc ccgtcttttt ttacccattc ccggttatct tgaataaatt
      481 acctagtcat ataacacact tcttgccgca ctggcattca tcgcttttca ggcacaggcg
      541 gtgaacgtca ccgtggcgta tcaaacctca gccgaaccgg cgaaagtggc tcaggccgac
      601 aacacctttg ctaaagagag cggggcgagc gtggactggc gtaagtttga cagcggagcc
      661 agcatcgtgc gggcgctggc ttctggcgac gtgcaaatcg gcaacctcgg ttccagccca
      721 ttagcggttg cagccagcca acaggtgccg attgaagtct tcttgctggc gtcaaaactg
      781 ggtaactccg aagcgctggt ggtaaagaaa actatcagca aacccgaaga tttgattggt
      841 aaacgcattg ccgtaccgtt tatctccacc acccactaca gcctgctggc ggcgctgaaa
      901 cactggggga ttaaaccagg gctagtggag attgtgaacc tgcagccgcc cgcgattatc
      961 gccgcatggc agcggggtga tattgatggt gcttatgtct gggcaccggc ggttaacgcc
     1021 ctggaaaaag acggcaaggt gctgaccgat tctgaacagg tcgggcagtg gggtgcgcca
     1081 acgctggacg tctgggtggt gcgcaaagat tttgccgaga aacatcctga ggtcgtgaaa
     1141 gcgttcgcca aaagcgccat cgatgcacag caaccgtaca ttgctaaccc tgacgcgtgg
     1201 ctgaaacagc cggaaaacat cagcaaactg gcgcgtttaa gcggcgtgcc tgaaggtgac
     1261 gttccggggc tggtgaaggg aaatacctat ctgacgccgc agcaacaaac ggcagaactg
     1321 accggaccgg tgaacaaagc aatcatcgac accgcgcagt ttttgaaaga gcagggcaag
     1381 gtcccggctg tagcgaatga ttacagccag tacgttacct cgcgcttcgt gcaataaaag
     1441 gaggcgcaga tgctgcaaat ctctcatctc tatgccgatt atggcggcaa accggcactg
     1501 gaagatatca acctgacgct ggaaagcggc gagctactgg tggtgctggg gccgtccggc
     1561 tgcggtaaaa ccaccctgct gaatctgatt gccggttttg tgccttatca gcatggcagc
     1621 attcaactgg cgggtaagcg tattgaggga ccgggagcag agcgtggcgt agtttttcag
     1681 aatgaagggc tgctaccgtg gcgcaatgta caggacaacg tggcgttcgg cctgcaattg
     1741 gcaggtatag agaaaatgca gcgactggaa atcgcgcacc agatgctgaa aaaagtgggg
     1801 ctggaaggcg cagaaaaacg ctatatctgg cagctttccg atggtcaacg tcagcgggtg
     1861 gggattgccc gcgcgctggc ggcgaatccc cagctgttat tactcgacga accgtttggt
     1921 gcgctggacg ccttcacccg cgaccagatg caaactctgc tgctgaaact ctggcaggag
     1981 acgggcaagc aggtgctgtt gattacccac gatatagaag aagcggtgtt tatggcgact
     2041 gaactggttc tgctttcatc cggccctggc cgtgtgctgg agcggctgcg gctcaacttt
     2101 gctcgccgct ttgttgcggg agagtcgagc cgcagcatca agtccgatcc acaattcatc
     2161 gccatgcgcg aatatgtttt aagccgcgta tttgagcaac gggaggcgtt ctcatgagtg
     2221 tgctcattaa tgaaaaactg cattcgcggc ggctgaaatg gcgctggccg ctctcgcgtc
     2281 aggtgacctt aagcattggc acgttagcgg ttttactcac tgtatggtgg acggtggcgg
     2341 cgctgcaact gattagcccg ctgtttttgc cgccgccgca acaagtactg gcaaaactgc
     2401 tcaccattgc cggaccgcag ggatttatgg acgccacgct gtggcagcat ctggcagcca
     2461 gcctgacgcg cattgtgctg gcgctactcg cggcggtggt gatcggtatt ccggtcggga
     2521 tcgccatggg gctaagcccg acggtgcgcg gcattctgga tccggtaatc gaactttatc
     2581 gtccggtgcc accgctggct tatttgccgc tgatggtgat ctggtttggt attggtgaaa
     2641 cctcgaagat cttactgatc tatttagcga tttttgcgcc ggtggcgatg tcggcgctgg
     2701 caggggtgaa aagcgcgcag caggttcgca ttcgtgccgc ccagtcgctg ggtgccagcc
     2761 gtgcgcaggt gctgtggttt gtcattttgc ccggcgcgct gccggaaatc ctcaccggat
     2821 tacgcattgg tctgggtgtg ggctggtcta cgctggtagc ggcggagctg attgccgcga
     2881 cacgcggttt aggatttatg gttcagtcag cgggtgaatt tctcgcgact gacgtggtac
     2941 tggcggggat cgcggtgatt gcgattatcg cctttctttt agaactgggt ctgcgcgcgt
     3001 tacagcgccg tctgacgccc tggcatggag aagtacaatg agtgaacgtc tgagcattac
     3061 cccgctgggg ccgtatatcg gcgcacaaat ttcgggtgcc gacctgacgc gcccgttaag
     3121 cgataatcag tttgaacagc tttaccatgc ggtgctgcgc catcaggtgg tatttctgcg
     3181 cgatcaagct attacgccgc agcagcaacg cgcgctggcc cagcgttttg gcgaactgca
     3241 tattcaccct gtttaccctc atgccgaagg ggttgacgag atcatcgtgc tggataccca
     3301 taacgataat ccgccggata acgacaactg gcataccgat gtgacattta ttgaaacgcc
     3361 acccgcaggg gcgattctgg cagctaaaga gttaccttcg accggcggtg atacgctctg
     3421 gaccagcggt attgcggcct atgaggcgct ctctgttccc ttccgccagc tgctgagctg
     3481 gctgcgtgcg gagcatgatt tccgtaaatc attcccggaa tacaaatacc gcaaaaccga
     3541 ggaggaacat caacgctggc gcgaggcggt cgcgaaaaat cctccgttgc tacatccggt
     3601 ggtgcgaacg catccggtga gcggtaaaca ggcgctgttt gtgaatgaag gctttactac
     3661 gcgaattgtc gatgtcagcg agaaagagag cgaagccttg ttaggctttt tattcgccca
     3721 tatcactaaa ccggagtttc aggtgcgttg gcgctggcaa ccgaatgata ttgcgatttg
     3781 ggataaccgc gtgactcagc actatgccaa tgccgattac ctgccacagc ggcggattat
     3841 gcatcgggcg acgatccttg gggataaacc gttttatcgg gcggggtgat acgagtgcgg
     3901 acggttccct cgcccccttg gggagagggt tagggtgagg gggcgttcac tgtactttca
     3961 acaggttaac tccccctttc tgagaggaaa aaaattaacg cagaatcttc ttctcagcca
     4021 aatccagcgc aaagtagctg aaaatcagat ccgcacccgc acgcttaatt gaacctaagc
     4081 tttcgagcac gactttctct tcatctatag cacccgccag cgcggcgaac ttaatcatcg
     4141 cgtactcacc gctcacctga tacgcgccaa tcggcaattc agtacgttca cgcagctcac
     4201 gcacgatgtc gaggtacgct ccagcaggtt taaccatcag gcagtctgcg ccctgggctt
     4261 catccagcag tgactcacga atcgcctcgc gacggttcat tgggttcatc tgatagcttt
     4321 tgcggtcgcc ttttaatgcg cttcctgcag cttcacggaa cgggccataa aaggaagagg
     4381 cgaacttggt cgaatacgac ataatcgccg tatctttaaa gcctgcagcg tccagcgcct
     4441 gacgaatcgc ctgtacctgg ccgtccatcg cggcggaagg ggcgatgaag tctgcgcctg
     4501 cagcagctgc aaccacggct tgcttgccta aattttccag agtcgcgtcg ttgtcgacgc
     4561 catgctcgca cagcacaccg cagtgaccgt gagaagtgta ttcacagaaa caagtgtctg
     4621 acatgacgat catttctggc acagtctgct tgcagatgcg cgacatacgc gccaccagtc
     4681 catcttcccg ccaggcgtcg ctgccggttt catcggtatg gtgagagatg ccaaaagtca
     4741 ttacggaacg gataccggcg ttggcgatgc gttcaatttc gcgtgccaga tgtttctctg
     4801 gaatgcgcat cacgcctggc atggcttcaa cggctttgta gtcgtcaatt tcttcttcaa
     4861 caaagatcgg caacaccagg tcgttaaggc taagtgttgt ctcttcaaac atagcgcgca
     4921 gcgcaggaga tttgcgcagg cgacgagggc gttggattaa gtctgtcatg gtctgcctga
     4981 tgtttgtgga attaaggtgc acagtatacc tgaagcgggg caggagtgtt ttacgaaagt
     5041 tgtcgcgatg ttgacaagaa gagaatgaaa gagagaccag gacatctttg taatagaaat
     5101 agcaataatt tcatattcac tgaaaatatt tttaatcttt atttatagct cttggttatt
     5161 attttttggc gtttggttgt gctgctataa attgaat
//
LOCUS       DAZSIO010000215         5166 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZSIO010000215 DAZSIO010000000
VERSION     DAZSIO010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.218"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1309..1518
                     /locus_tag="ACHX5T_003741"
     CDS             1309..1518
                     /locus_tag="ACHX5T_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468742.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTD
                     EHVPLYIRRLEYYRLLYRPKQTESY"
     gene            complement(1584..2117)
                     /locus_tag="ACHX5T_003742"
     CDS             complement(1584..2117)
                     /locus_tag="ACHX5T_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468743.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVT
                     LEDKLVEKPVTGKSRKN"
     gene            complement(2120..3046)
                     /locus_tag="ACHX5T_003743"
     CDS             complement(2120..3046)
                     /locus_tag="ACHX5T_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFR8468744.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSG
                     SLTLTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQNRTAPERVKSPSRGFSMGF"
     gene            3223..3621
                     /locus_tag="ACHX5T_003744"
     CDS             3223..3621
                     /locus_tag="ACHX5T_003744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468745.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQER
                     KLLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTK
                     TGELCYKKDRILQALKELKYDLETSPNPDA"
     gene            complement(3825..4739)
                     /locus_tag="ACHX5T_003745"
     CDS             complement(3825..4739)
                     /locus_tag="ACHX5T_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR8468746.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLS
                     ASERRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPV
                     PLSLAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVAT
                     SARTLKPWEALGISRAWYYQLKKRGLVE"
     gene            complement(4938..>5166)
                     /locus_tag="ACHX5T_003746"
     CDS             complement(4938..>5166)
                     /locus_tag="ACHX5T_003746"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468747.1"
                     /translation="SWRDVKFGNRKPRQSATHQGRKLVVLSRSVFRTHETDRTSLSCI
                     VMQRPENDSDAFFWFGLVFSCLFAFFAPESD"
BASE COUNT         1434 a         1352 c         1105 g         1275 t
ORIGIN      
        1 aaaaaaaaac cgacctgggg tcggtggcaa gaatcagagt ctatgggaaa tattttagtt
       61 atcggatgct caatattcac aaacgcaaaa aatgcctcag tgatgcccta aaacaacctt
      121 tccagaagga gtccgcgctt tacgatgcac ggaagagata aaaaacccca caaaagtggg
      181 gaaccaagag aaacaacaat tacaggttgt tggttgtgtt cgaacaacga tgaagtagct
      241 acgctaaata ataattaccg tacaggccct acatattcaa cactaaattt aagatcctga
      301 cgaaggattt ataaatgcac gaaaacacat agacagacaa attcactaaa ttcatacaca
      361 aaaacaagtg tagttttcta agtaaattag attcgacaac caattagttt atccacatcg
      421 cattccactc ttgctacacc aatcacattt cttgatatgt aatagcagaa aaaataaaac
      481 cagacaccaa tagaccaaaa aacaaacatt caacaagaaa cattttgaac aatatcacaa
      541 aactgtcaca accatcatca gactattgaa aaaaactggt tgctgtttta taaaagcgac
      601 tgtaacaacc tccactctat agttccatta gtttaaagtg tcacacaaac tgagcatcca
      661 tttgttttcc ttacttttgc ccctgtattc caaggggttt ttttataact taaagattag
      721 cacaccacac aaaaacaata aaaaaaacga caagttatac aataaaacca gtactatcca
      781 tacagctaat tacatataaa tactattaaa ccatctggac attaaaaata aactcttata
      841 acataaaatt aatgcggttc cggtaccata tgtcagggat aatgtgcaaa tggaggtatc
      901 ggaactgcat ttcagttcat tattaattaa tgacaaaaaa aacctctgcg agcgcagagg
      961 gggtaaggaa tgttcccggt cataatatgt cgatagtgtt tatatacata cagaacaaga
     1021 acaacaacca gccaactaat aaatatttta ttttttagag ctgaaacctt aaagctcact
     1081 tccattccat acattgttat aaaccttatg ttgaaaactg aaagtaagta cggcagactt
     1141 attaagttgc gtgcagtctt ttcacgttat acaaacaaga acacctgcga agttatttct
     1201 ccttacatat ccccctatgc caacggggga ttttttctgg ttcactaacg cataaataat
     1261 agtgacaccc tatcattcac aaaccgtgat aatataagga ttatcatcat ggcaaaatta
     1321 ggtgaaaaca ccccacatat tatcgatgct actgtagatt ttatggcatc aagccaggca
     1381 tttcgtgagt atctgaaaaa acttccccct gataacacaa taccgccagc tcttactgat
     1441 gagcacgtac cgttatatat acgaaggctg gaatactatc gtctgcttta tcgaccaaag
     1501 cagacagaat catattgaaa aaaacggtga acggttcgcg aaatcctttc gcgggtcgtg
     1561 aggcgttgga caagcgaagc gcgtcagttt ttccggctct tccccgtgac aggtttctcc
     1621 accagtttat cttccagtgt cacaactttc tgatacaccg ccgctgtggc gttgtagttc
     1681 ctgtcgagta ttgttttcat tccccatccg agcacacaga acataaccag aaaagccact
     1741 gacaccccac ccagccattt ccagctatac cctttaaggg tgttgtattc ctgcacgata
     1801 ttgctgtgaa ttgtcgcaat atccctgttc agggcatccg cgtttgcctt catgtttgcg
     1861 actgactctt ccagccgctg aaaatcctgg ctgatgcgct cacctatcag tcctgccacg
     1921 tccagcttct gcacctcatt tctgacttca tccctgacct gttttgccac accgtcagaa
     1981 atatcccgat atccgcgaag agtttccctt gccacagcca gagaatcagc aacagctttc
     2041 tgttgctcca ttgcggtatg ttgcagatgc tccaccgata acaggagcgt gagtaatttt
     2101 tcttctgtgc tggccatgtt cagaatccca tagaaaatcc acgtgacggt gatttaaccc
     2161 gttcaggtgc agttcggttc tgtctgctca ctctgtctct catgtcaaag ccatgctccc
     2221 gcgcaacatc atgttgcagc cttctgacca gttcatgttg cttaaccgga gtgtccagcc
     2281 attccctgat gtcatgcccg accgttcgcg cctgttcaag atggcgggaa tagtccggtt
     2341 cagaccgttt ttgcatgatg tcatgacgct gggcatcatc aagccgtccg gcagcttcaa
     2401 aacccagatg cacttcggga cgtcgttcaa tgccctgttc tttcagcgtc cggcagtcaa
     2461 tgaagcccct ttcaaagcca tgctttctca tatgttcgtt gtgcttcacc tcccagcgtt
     2521 ttcgcaattc ccgcaactgc tctttttgct gagtgagagt caggcttccg gaatcctttt
     2581 tcgcacctcc ccgctcggga tacctggcgt tatagcgccg gaaatactgt tccggtgtac
     2641 gttcaatgcc atcattcaca cgctgactca tcatgatatg cgcatgaggc tgttcgccgc
     2701 cgtcaatgct cgctttcgga ttgtgaatgg caaaactcca ggcgtgccgt tcgccagctt
     2761 cctgacgtac aaaatcctgc accagttcaa ggcgctgttc cggtgtcagt tcacggggta
     2821 atgcaatttc aagttcccta taggtcgaac cgttcgcacg ttcaaactgg tcagccgcct
     2881 gccagaaatg ggaaggatta tccctggccc attccggcat gtttccgtgt gcggaatatt
     2941 ccaaatcctg acggtcgcgg tatttctctt tgcgctcgat gtaatcggcg tggtttgcag
     3001 cctgcccctt gccaccaaat ttaacgctga ggtgataggt tgccacggtt tcagccctct
     3061 gcaggagcgc actgtttctc gacgaaggag agaaacatat aagtgcgctt ccttctaatt
     3121 ttacgaatta gcacggaaga taccccctgc acccgccgac cgcaagcgat atactgcacg
     3181 accacaacac accgcaaaaa aacaccaggg agcatcacga acatgcgcat cagcaacatc
     3241 gaatggctga agaaacggat cggattcatc aggaagctcg gagaacagac cgcacgacag
     3301 agacagatta ttgacctgat cgacaacgaa gccggactca cagaacagga acgcaaactt
     3361 ctccacgtac tggcaacagc ggaaaaaaac gacttacagg cacaggagag cgaacgaaaa
     3421 caggcggtcc aaaaacgaat cgagggaaaa aaacaacgac gggaacgcaa tcaccggctc
     3481 tttctcgcgg ctgggctact gatcgaagca ggactggtag acacaaaaac aggcgagcta
     3541 tgctataaaa aagacaggat attacaagca cttaaggaac ttaagtacga tctggagaca
     3601 tccccaaacc cagacgcgta agtgcctcca tccgtgttcc gattccctgg tgcttcctcg
     3661 ctcattcgct cgctacgctc ggtcatgaga ctgcggcgag cactaccacc acaatcacgc
     3721 ggaaagccgc gccattgcag ggctgaacgg ggaaaaacgg caaaaaacct gctgctcaaa
     3781 aagacgccag aaaggcgctg ttatctgata taggcttatt tggtctactc tacgagacct
     3841 cgttttttca gttggtaata ccaggcgcga ctgatcccta atgcctccca cggtttcagc
     3901 gtgcgtgcag atgtagctac tgtgctgcgc ttagacttag aaccgccctt gcgaccgcgt
     3961 gcagcctgaa tttctggcgt gtgcgtatca gccacatact gtgcgaaagt ttccggcgtg
     4021 aagttcctgt gcgtgtattt cgcaatgctc ttgccaatag cccgacattc agccagtgaa
     4081 agcggaacgg gaagcgatgc attgtacatt tcgacacgct ggatcaccgc ttcaagccac
     4141 tgtgagaacg ctggccagcc ctgacgaatc gccctgtaag cccatttgcg cgtcatttcg
     4201 aacaggtggc agtttcgccc catcccgtaa tgtttatcga tgctacgacg ctctgaggcg
     4261 ctcaaatcga gataatcagc cagttcatcg agggtatagg cttcctcgcg ccactccatc
     4321 accagccatt ccaggtggaa cggatttttg caaatcaggc cgctgtagtt cacatccgca
     4381 caaagtttct cacacagcga acgttcaacc gctgcggcat atttcagcgc cttaaccgag
     4441 gcatcaggcg cggttctcac ggcgatgttg agtgcgtaga gcaggtgagc gtgtccgttc
     4501 acaggatttt taacggtgat gttcggcgcg ggagcgttcc ggtcgctcca gtcaatcgca
     4561 gccccctcgc ggtcaacgtc aaaaaccagc cagaactgcg catgaggctg gttctgctgt
     4621 atgtaccgcg caagaagcgc acgccctttg ccggagatgc gaagaccaaa agcgagatca
     4681 tccgtgtgat agggacgatg gggtaaattt tcgtcgaagt attgaagcgc ggcgctcatg
     4741 tgttacgatt ctctccgggt tatgcgacct acacgccctc tgtatttcat gttgaatctt
     4801 gccggatctg gacatgattt caggggcgtt tgtttttcag tcgcttacac tacacccaac
     4861 ggcaaatcct gtcgaacctt cccgaaaacc cgatccattt accataactc ccgttgtacg
     4921 ccccccttaa aacgccctca gtcgctttct ggcgcaaaaa acgcaaaaag acaagaaaac
     4981 accaggccaa accagaaaaa cgcgtcagaa tcgttttcag ggcgttgcat aactatgcac
     5041 gaaagcgatg tgcgatcggt ttcgtgcgtt ctgaacaccg aacggctcaa aacgacaagt
     5101 tttctcccct ggtgcgtggc gctctgacgg ggtttcctgt ttccaaactt cacatctcgc
     5161 caggac
//
LOCUS       DAZSIO010000216         5163 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZSIO010000216 DAZSIO010000000
VERSION     DAZSIO010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.219"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..220
                     /locus_tag="ACHX5T_003747"
     CDS             <1..220
                     /locus_tag="ACHX5T_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8468748.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(392..721)
                     /locus_tag="ACHX5T_003748"
     CDS             complement(392..721)
                     /locus_tag="ACHX5T_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR8468749.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(726..911)
                     /locus_tag="ACHX5T_003749"
     CDS             complement(726..911)
                     /locus_tag="ACHX5T_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR8468750.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(908..3547)
                     /locus_tag="ACHX5T_003750"
     CDS             complement(908..3547)
                     /locus_tag="ACHX5T_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR8468751.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3755..4744
                     /gene="ldhA"
                     /locus_tag="ACHX5T_003751"
     CDS             3755..4744
                     /gene="ldhA"
                     /locus_tag="ACHX5T_003751"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR8468752.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4855..>5163
                     /gene="hslJ"
                     /locus_tag="ACHX5T_003752"
     CDS             4855..>5163
                     /gene="hslJ"
                     /locus_tag="ACHX5T_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR8468753.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     N"
BASE COUNT         1307 a         1323 c         1203 g         1330 t
ORIGIN      
        1 tttgtaccga atgtttgccg ataaaggttt ggtagtcgcg caatatattc gtaaccgtcg
       61 tctcgatttt tgtgcagatg cgattcgcca tgccgcagat gatgaaaaac tggcaggcat
      121 cggctttcat tggggatttt ctgaccagag tcatttttca acggtattta agcaacgctt
      181 tgggatgacg ccaggcgagt atcgacgtaa attccgctaa ctaattggcg ttgcatgtac
      241 tgcaacgcca attagttaaa acagatattt gactatgcat ttttcagggc gaaaggcaat
      301 ttgacaggag tttttgaata ttcaccttgt ttcaggaaaa tagatcaatc gctttctcct
      361 gtagcgttat cccgatattg cggttttgct attaaaactt tcgcacccat ttaccgttta
      421 tcccttgtac atattccccc ggtttggccc gagctacgag cttttgcccg gcgagtttcg
      481 cgatatcatc tacagacaca ttattttgct ttgcccgttg ttggtaactc gctttacgtt
      541 cggcattaat ttcggttact aatttctctg tttcagcatc cgttttcagc gcaaccagat
      601 aaccataaaa tgtttcacct acccgcccct gagttctggc ttcatccaga gtcaatgcca
      661 ttacattgct gcttaccagc ccaacaagaa acgcacaaag aagtaatgtt ttcttcatca
      721 tcgcctcaga aaagatcgct acgagtttca agcagctctt cgacatcttt gtctgccttg
      781 atgatgatct catgctcaat tttaacgttc atattgatag tgatcggttc cttaggtgca
      841 gcgacttcaa tgcgaggcgt acagccaacc agcataaaag atgacgtcaa cgcagccagt
      901 aaaattttca ttgtttttcc tcacactctt ttccttgcgg acagtcattt cccggcagac
      961 gtgcgttctg ctccaaccat gcctggagat tatcgccaaa gcgtaaactg cgccacagcg
     1021 taaaaatatt ctcttcatga tggtaattaa ggtttaccgt accgctttta ccatcaacgc
     1081 gactggtacc tgtaatgttg gcctgcatgg ttaataaacc gagattatct aagttaattt
     1141 ttgtcgatga ccggctaatt tccatatagc gcaaccagtt aattgctgaa cccgcagtca
     1201 tattgtcttt caccaccgca tctgcggtat ctttatccag ccgcaaggtc attagcccgc
     1261 tatttgccag ccaaccatct ttcactatcc atttttcgtt gtttagccat aacggcaatg
     1321 caccactaaa tgccccggac atggcgaatt gtttcggatt gacggcgcta accagttcgc
     1381 tggatgagag attattcagc cgcaacagcg ccgggtcatg ttgcggcata cgtaattgtt
     1441 tcatcagcac gttaccgcct aacacatcga cgctaacatc agtcaacagc aagggttctt
     1501 cttcagtcca cggataacgc ccttgcaaat cagccgtaat atttttcgct gtcaccagat
     1561 taatcacttc ggcaattcgc aacgtaacgg ggccgcgagt ccccagatgc caggctccat
     1621 cggcaaaacg gaaaggcagg acaaaatcga caccgttaac ctgattatct ggcatccagg
     1681 cactaccgcc tttcaacacg ccgtgccctc ccgcgcggaa tccttgttca ggcgcagcag
     1741 aaaatgcaac ctgagcatac agttcaccat cgcgtaagtt catcttccag tcgggtggca
     1801 ccagcggctg gaatacagtc agtgattgtt ttggccacca ggcgttaccg cgcagacgaa
     1861 taccgtccca gcgaccattt acccgaaccg ggccaatctc accagcatgt aaatcgcctt
     1921 taaagaggaa ataggtcgga ttgcgcccat cgacgctaaa ttttaaggtt gatggcggta
     1981 acacactgcc gccagtgaac agcgtttgcc cggcgtccag tgacagcgcg ccgctgaagg
     2041 agggatgctg tgcgtcacgt acccagacga cgggcttgtc gagaattaat cgcggctttt
     2101 ctaccgtcat cgtaccgtat tgtaactggt cgaaaccggt ggagagatcg gtcagcgtaa
     2161 tcgtgctgtc atgccactca ccttttcctg cgacatccca ggtggcattc atcggtgtaa
     2221 aactcccttt tccccagtag cgccactgcc agcggccagc gtcagggaga aaatcattcg
     2281 ccagcccatc catatgcagc acgaaatcgc ccagttcatt ttcatgcgcc tgcaaaatgg
     2341 cctgcaaacg tccgtcaaca ccacgttggg tgacttttac acccgctaaa ggccagcgga
     2401 tttcatcgat gtccagcgaa tcgatgactc ttccctttga acgaagtaac gcgcctggct
     2461 caaaggtcag cgttgggtca gacagacttc cacttagctg cgcaggtaaa cgggcatata
     2521 aaatgaggtc cgcctgtttc gcttcaccgg tcagctgcag aggcagttga ctgttatcca
     2581 tgcttaattt tcctgggcca aaattaagca ccgcgttgcc cttacccgct tgcccctggg
     2641 tcagcacatt cagtcgtccg ctgaccagag cgttctcaag ccctgcctgc cagttgtcga
     2701 ctttgacacc cagtcggcca ctcaaaggaa aaccagcata cagccagctc cagcgaccat
     2761 cgcttacggt caattgttga cgagtaattt gccacggcaa atcgagcaac ggatcgccgt
     2821 tatcccgtgc cagcacgatc aattgcccgc tattttcctg ccagtccagc tcggcatcca
     2881 ccagtgacgg ttcctgcggc aagtttaacg tcgcagtagc atgcccactt acaggaagtc
     2941 catccggcac gagcggcata gtaaattccc ccaccagttt aaccggcggc tgattttcaa
     3001 acgcgacgac atccagttcg ctgactgtaa gttgttgccc tttcagctgg ccttgaaatt
     3061 taactttttc gccctgataa cgcagttgct ggatatcaga ggttaatgcg agagagagtt
     3121 ttccctgcca ttcctgccag ggagaaaaaa tcagtttatc gatattgatc caggtgttag
     3181 gcagcatgga ctgccactgc gcgagggttt ttggagcagc tggcgattgc tccgtctgcg
     3241 gcaatttcgc cagacaagca gaatcaagtt ctaccgtgcc gacgttcaat aaccagcggc
     3301 tgggatgtga aaggctggcg ttggtgatat gcgcaagctg acaatctccc accagataac
     3361 ggagatcggg aatgattaaa cctttacgcg taatgcgtgg gctttcatct aatgcaatac
     3421 gtgtcccgag cggtagccag atgtccgcca gcgtgggaac ccacagcccg agcgtcatca
     3481 gcagcgtcaa cggcacaaga ataatcagta ataacagcgc gagaacggct ttatatttac
     3541 ccagcatggg tagttaatat cctgatttag cgaaaaatta agcattcaat acgggtattg
     3601 tggcatgttt aaccgttcag ttgaaggttg cgcctacact aagcatagtt gttgatgaat
     3661 ttttcaatat cgccatggct ttcaattata tttgaaattt tgtaaaatat ttttagtagc
     3721 ttaaatgtga ttcaacatca ctggagaaag tcttatgaaa ctcgccgttt atagcacaaa
     3781 acagtacgac aagaagtacc tgcaacaggt gaacgagtcc tttggctttg agctggaatt
     3841 ttttgacttt ctgctgacgg aaaaaaccgc taaaactgcc aatggctgcg aagcggtatg
     3901 tattttcgta aacgatgacg gcagccaccc ggtgctggaa gagctgaaaa agcacggcgt
     3961 taaatatatc gccctgcgct gtgccggttt caataacgtc gaccttgatg cggcaaaaga
     4021 actggggctg aaagtagtcc gtgttccagc ctatgatcca gaggccgttg ctgaacacgc
     4081 catcggtatg atgatgacgc tgaaccgccg tattcaccgc gcgtatcagc gtacccgtga
     4141 cgctaacttc tctctggaag gtctgaccgg ctttactatg tatggcaaaa cggcaggcgt
     4201 tatcggtacc ggtaaaatcg gtgtggcgat gctgcgcatt ctgaaaggtt ttggtatgcg
     4261 tctgctggcg ttcgatccgt atccaagtgc agcggcgctg gaactcggtg tggagtatgt
     4321 cgatctgcca accctgttct ctgaatcaga cgttatctct ctgcactgcc cgctgacacc
     4381 ggaaaactac catctgttga acgaagccgc cttcgatcag atgaaaaatg gcgtgatgat
     4441 cgtcaatacc agtcgcggtg cattgattga ttctcaggcg gcaattgaag cgctgaaaaa
     4501 tcagaaaatt ggttcgttgg gtatggacgt ctatgagaac gaacgcgatc tgttctttga
     4561 agataaatcc aacgacgtga tccaggatga cgtattccgt cgcctgtctg cctgccacaa
     4621 cgtgctgttt accgggcacc aggcattcct gacagcagaa gctctgacca gtatttctca
     4681 gactacgctg caaaacttaa gcaatctgga aaaaggtgaa acctgcccga acgaactggt
     4741 ttaatcttgc agctcccctg caatccaggg gagctgattc agataatccc caatgacctt
     4801 tcattctcta ttcttaaaat agtcctgagt cagaaactgt aattgagaac cacaatgaag
     4861 aaagtagccg cgtttgttgc gctaagcctg ctgatggcgg gatgtgtaag taatgacaaa
     4921 attgctgtta cgccagaaca gctacagcat catcgctttg tgctggaaag cgtaaacggt
     4981 aagcccgtga ccagcgataa aaatccgcca gaaatcagct ttggtgaaaa aatgatgatt
     5041 tccggcagca tgtgtaaccg ctttagcggt gaaggcaaac tgtctaatgg tgaactgaca
     5101 gccaaagggc tggcaatgac ccgtatgatg tgcgctaacc cgcagcttaa tgaactcgat
     5161 aac
//
LOCUS       DAZSIO010000217         5150 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZSIO010000217 DAZSIO010000000
VERSION     DAZSIO010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.220"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            189..446
                     /locus_tag="ACHX5T_003753"
                     /pseudo
     CDS             189..446
                     /locus_tag="ACHX5T_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(496..1446)
                     /gene="uspE"
                     /locus_tag="ACHX5T_003754"
     CDS             complement(496..1446)
                     /gene="uspE"
                     /locus_tag="ACHX5T_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR8468754.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1598..2350)
                     /gene="fnr"
                     /locus_tag="ACHX5T_003755"
     CDS             complement(1598..2350)
                     /gene="fnr"
                     /locus_tag="ACHX5T_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR8468755.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2545..3060)
                     /gene="ogt"
                     /locus_tag="ACHX5T_003756"
     CDS             complement(2545..3060)
                     /gene="ogt"
                     /locus_tag="ACHX5T_003756"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR8468756.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTRYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3071..3214)
                     /locus_tag="ACHX5T_003757"
                     /pseudo
     CDS             complement(3071..3214)
                     /locus_tag="ACHX5T_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            3214..3430
                     /locus_tag="ACHX5T_003758"
                     /pseudo
     CDS             3214..3430
                     /locus_tag="ACHX5T_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885449.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(3604..4419)
                     /locus_tag="ACHX5T_003759"
     CDS             complement(3604..4419)
                     /locus_tag="ACHX5T_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8468757.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4516..>5150
                     /locus_tag="ACHX5T_003760"
     CDS             4516..>5150
                     /locus_tag="ACHX5T_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR8468758.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1374 a         1174 c         1225 g         1377 t
ORIGIN      
        1 ctattttacc gcgacaattc attcagaaca tcaatagtca gggaaggaag tagcaacatt
       61 agctaactaa tcaatgcgtt gattgtaaat ccagctaaga ggtgaggttt tcagagcaga
      121 caacggtgaa acgtcatggt attgttacgt ttaggtaaca agaaatttgt ctgcacaagg
      181 attacatcat gattatggcg aaactgaagt cagcgaaagg gaagaaattt ctctttggtt
      241 tgttggcggt tttcattatt gcggcgtcgg ttgtgactcg cgcgaccatc ggcggcgtta
      301 tagaacagta caatattccg ctgtctgagt ggacgacatc aatgtatgtg atttagtcat
      361 cgatgatttt tgtttatagc ctggtcttta ctgtgttgct ggcaatcccg ttgggaattt
      421 atttccttgg cggcgaagag cagtaagtaa aaaataggcc cgataactcg ggccttgtca
      481 gttattgaag agtcgttaat cgtcttcttc gtcatccagt tcaacgggtg tctgatactg
      541 gtcaggttta atgaccagca ggtcgcagcg aagatgatca atcacctgtt ccgccgtgtt
      601 gccgaggaat gctgctgaaa taccggtgcg tccgaccgtg cccagaacca caattcccgc
      661 ctgtaaatgc tccgccaaat caggaatcac ctcttcgggc agaccttttt ctacgtgcgt
      721 catgttttca ttaatgccga atttctgccg cagggctttc attgccagca aatgttgccc
      781 acgaatggca tcgttataaa cgctcgggtc aaattccggc agttcaatcg cgatattaat
      841 tggcgttacc ggataagcgc caaccagatg aacttcggta tggttgactt gttctgccag
      901 ttcgatcgtc tctttgacca gtttttcatt gagcgcatta tgatacggct cttcactggc
      961 gagattcacc gccaccagcg ccttgcctcc ttccggccac ggctggtctt tcaccatcca
     1021 caccgggctt gggcatttgc gtaacagatg ccagtccgtt ggcgtaaaaa tcaccgcttc
     1081 cagacggtca tgttggtgcg ccatttttag caccaaatcg tgtccgccgc tgatcacttc
     1141 ctgaatgatg gcttcgaaag gacggttatg ccagaccact ttaatttcaa tgggaacgcc
     1201 agcattgaga taatattttg cctgctcgtg gatccaggct gtacgctggc tgatgacgcc
     1261 ctgacgcata gcggtacgtt cgtccgggga gagcagggtg gtcatttcgt atgagaagtc
     1321 atagatcggc aaaaaggctt taattttgcc accaatccgt tgatgtaaat aaacagctcg
     1381 ccgcaatgct ggttggtcgt cctggttagg atcgataaca acgagcatgt tctgatacat
     1441 agccatacag ggtctcctta caacaactgt caacgcagtt tgtaattaaa agattaaccc
     1501 atatctggtg aatgaaacag tgatgaacct tctgccagat caataaatca gaaaaattta
     1561 atgatatgac agaaggatag tgagttatgc ggaaaaatca ggcaacgtta cgcgtatggc
     1621 cagcaagctg ggccagcgca tcgttatttt cgatggtgat gtatttacct ttgactgcca
     1681 gcataccgct tttctggaag cgacccagca gacggctgat ggtttctacc gtcaggccca
     1741 gatagttacc aatatcgcca cgagtcatcg tcaggcggaa ttcacgaggg gagaagccgc
     1801 gttgggcaaa acgacgggac aggttgtaga tgaatgcagc cagacgttcc tcggcatttt
     1861 tcttcgacaa cagcaagatc atgtcctgat cgcctttgat ttcaccgctc atcagacgca
     1921 tcatctgctg acgcagattc ggcattttac cggacaaatc gtccagcgtt tcgaacggaa
     1981 tttcacatac catcgaggtt tccagcgcct gagcgaagct cgggtgatgg ccgctgccga
     2041 tggcgtcaaa tcccactagg tcgcctgcta aatggaaacc agtgatttgc tcgtcgcctt
     2101 gctcagtgat tgtataactt ttaatcgtac cggagcggat ggcataaagc gatttaagtt
     2161 catcaccagc cttaaacagc gtctggcctt tctgaatagg cttcttccgc tcaatgatat
     2221 tatcaagctg atcaagctca tgttcgttga gtgtgaacgg gatgcaaagc tggctgatgc
     2281 tgcaatcctg gcaatggata gcacaaccgc cagactgaat gcgccgtata attcgctttt
     2341 ccgggatcat aggtctgctc aagccgtaat tgatatttgt caattttaac atctttttag
     2401 ggagcaagta agtctaagca aaccttaaca gcagagaatt ccgatattag atgtaaatat
     2461 atgtctatct atttgaaact ccttaagttg tcaagggtaa ctttacataa aagtgtgaac
     2521 aagctggcac aaattgttta atgtttacag caaaagataa ccttcgtggc gcaataacca
     2581 ctcttttcgc tgaactccgc ctgcatatct ggtcatggtg ccgtttcggc caataacccg
     2641 atggcaaggt acgacgatgc tgatgggatt cgatccgttt gccgcaccaa cggcacgcgc
     2701 cgcgccaggg cggcccaatt gttcagccag ttggccgtaa tgcattacct gcccgcaggg
     2761 gatagtgcgt agtgttttcc agacttcgcg ctgaaatggc gtcccccccg tggcagtggg
     2821 aagcgtatca ataatgctaa gattaccggc aaaatattca cgaagcttgt cgcttaaacc
     2881 acctgggttg gtggcagaaa tgcgctcata gccttctttg cgataatgga tgtccagcag
     2941 ctgcaccatg cgttcgctgt actcttccca ttcaaccgcc cgcaggcgaa attgctcatc
     3001 gcaaatcacc cacagtggac ccagtggcgt ggcaattttt tcttcaagta atctcagcat
     3061 ccgttctctc ttaagacaaa cgtgggtaaa taccctgacc tatcggcaga ccgacaagat
     3121 accacgccag caacatcagc agccatacca ccaaaaagat aagtggatag ggcaagacta
     3181 acgaatagta agtacccagt ttcgcgtctg gtttgtcgca gtctgatagc ataaagcgat
     3241 tttttaattt ttctgcctga agaaatgagg gcaagccatc acatggatcg agggtgggat
     3301 aattccagtc agtgaaattt taccgtaggc tatctgttat ttgattctgt ggcgtgatag
     3361 ccgccagaca ccactgaact cgtcactcat tccccaatcc cggcgagaat gtggttattt
     3421 acagagctaa aaaatgaata aaaaaataga gcctgtcgac atccgccaga ctctacagta
     3481 cacacagcag tgcatcctcg tcttaaatcc ggcctggggt cctggtgacc ggtgacagtg
     3541 ttataacggt tttacccatc cgttgccaat tttcttcagt gacagatttc acgaaaatat
     3601 aagttaaatc atttgtcttg tgattgcata attatgcatc gttaatcgac gagtaaattt
     3661 accgggcgag tagcccgttc gacgcttaaa catattgccg aacgattcag ctgaggcata
     3721 gcccagacga tgggcaattt tggtcaacga atatccatca tcaagaagcg tcactgcaga
     3781 aataacgtgc atttgctgaa cccagtggcg aaatgttaat cccgtctctt taacaaataa
     3841 gcgcatgagt gtacggctac tgacgttaat tttatccgct agattatcca gtgttggcag
     3901 atacccttgg ttgtttagca aattctcaca aagctgctgt agccgctcat catttggcca
     3961 agggagttca agtgacattt tcacccccat ttttatttcc tgaagtatca gtgtctcgag
     4021 ggctgaaaaa cgtaactgtt gtttatctgt ggtactttgc gacggtggaa gttggttaat
     4081 tgcaattagt aattccctta acaggggtga aatacccgtc atgaagcaaa ctgttcccag
     4141 cgtttcactt tccgcttctg aaaccagtac ggtttttaaa agtacgttgc ctgtcgcaac
     4201 aaacttatgc ggcatttgtg ggggcagcca aagcgcgctg ctggcatgga ctagccagac
     4261 gctttctcca gcccatacac gaacgactcc ttcagccggg taaagcaact gggcatagag
     4321 atgtttatgc cagggaactg tacctccatt cagatgttcc gaatccagtc cgataatgtg
     4381 atgccctggt gatgctttgg gttgcagtgt aattgtcatt tacagtaggt attttgtcag
     4441 aaatcatcaa ataagccatg ttaaatttta aatatgacgt atgcaagttt ccggatatat
     4501 acggtgtaaa gagtgatgat aatttctaaa aaacagctta ttggggtggt agcgatcggt
     4561 attcttttga ctggcgttgt cttttttatc tggagggtaa gcaaaggaag atttatacag
     4621 acgacggatg atgcttacat tggtggtaat atcaccacgg tggcaagtaa agtttctggt
     4681 tatatttctg ctatagaggt gcgcgataat cagagcgtaa aaaaaggcga tattatcctg
     4741 cgtctcgatg atcgggatta tcgtgcaaat gtcgccaggc ttgaggcaaa aataaaaagt
     4801 agcaaagcca atctcgaagg cattcaagcc actattacga tgcagcagtc gataattcaa
     4861 agtgccagtg aaacatggca ggcagtaaaa catgaggagc aaaagcgcct gcgagatact
     4921 gaacgttatg aaaaactcgc acaatcagcg gcaatatcgc aacaaattat tgataacgcc
     4981 aggtttgact accagcaggt cgcagcaaag gagcgcaaag ccgcgaacga tttcctggtg
     5041 gagaagcaac ggctggcagt tttatctgct caggaagaaa acgtacgtgc gagtatcgaa
     5101 gaagttcagg ccgctttgac tcaggcacta ctggatctgg aatataccct
//
LOCUS       DAZSIO010000218         5142 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZSIO010000218 DAZSIO010000000
VERSION     DAZSIO010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.221"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(11..322)
                     /locus_tag="ACHX5T_003761"
     CDS             complement(11..322)
                     /locus_tag="ACHX5T_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR8468759.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="ACHX5T_003762"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHX5T_003762"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="ACHX5T_003763"
     CDS             701..1054
                     /locus_tag="ACHX5T_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFR8468760.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACHX5T_003764"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACHX5T_003764"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFR8468761.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="ACHX5T_003765"
     CDS             complement(2810..3280)
                     /locus_tag="ACHX5T_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR8468762.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACHX5T_003766"
                     /pseudo
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACHX5T_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4119..4337)
                     /locus_tag="ACHX5T_003767"
     CDS             complement(4119..4337)
                     /locus_tag="ACHX5T_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR8468763.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACHX5T_003768"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACHX5T_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR8468764.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         1396 a         1182 c         1223 g         1341 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtgcc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gaactatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttactgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca at
//
LOCUS       DAZSIO010000219         5125 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZSIO010000219 DAZSIO010000000
VERSION     DAZSIO010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.222"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..408
                     /locus_tag="ACHX5T_003769"
     CDS             <1..408
                     /locus_tag="ACHX5T_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR8468765.1"
                     /translation="GKWGEIAPFTVTPEMLEAAREARRQEIEAWRAEQEAKLFTFEWN
                     GRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADNQQVKLSMPELEELAAAMVQAQ
                     VDRNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(609..1466)
                     /gene="sitD"
                     /locus_tag="ACHX5T_003770"
     CDS             complement(609..1466)
                     /gene="sitD"
                     /locus_tag="ACHX5T_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR8468766.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1463..2320)
                     /gene="sitC"
                     /locus_tag="ACHX5T_003771"
     CDS             complement(1463..2320)
                     /gene="sitC"
                     /locus_tag="ACHX5T_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR8468767.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2317..3144)
                     /gene="sitB"
                     /locus_tag="ACHX5T_003772"
     CDS             complement(2317..3144)
                     /gene="sitB"
                     /locus_tag="ACHX5T_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR8468768.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3144..4058)
                     /gene="sitA"
                     /locus_tag="ACHX5T_003773"
     CDS             complement(3144..4058)
                     /gene="sitA"
                     /locus_tag="ACHX5T_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR8468769.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4490..>5125)
                     /locus_tag="ACHX5T_003774"
     CDS             complement(4490..>5125)
                     /locus_tag="ACHX5T_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR8468770.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1358 a         1251 c         1239 g         1277 t
ORIGIN      
        1 ggcaaatggg gtgagatagc cccattcact gtgacaccag aaatgctgga agcggccaga
       61 gaggccagac gtcaggaaat tgaagcatgg cgcgcagaac aggaggcgaa gctgttcacg
      121 tttgaatgga acggtcgtat ctggaatgct ggtcccgact cactgggccg cctgtccccg
      181 gtagtcatgc tggcaaaatc tgtcacagca caaacacata tggcgtggag cgatgccgat
      241 aatcagcagg tgaaactgtc gatgccggaa ctggaagaac tggcggcagc aatggtgcag
      301 gcgcaggtcg atcgcaacga tgagatttat cgccgtcagc gggagctgaa ggaagagctg
      361 aatagtctga aggatttgaa ttcggttagg aattttatcg tggaataaca gaagctgcgg
      421 cacgtcgtat gcaggaacgt gccgcggttg gctggtaaac tttcgatagt gcgagtattg
      481 aatgatttct aggcgttatc gattttacgt attttttgca tgatagaatt cgtacctcct
      541 cccaacgacc ttccatggct ttccggactt ctgtaggctg gcggacgatg ctgtcctcgg
      601 atgttgtgct aattgggtga taacagatcc tgtgtatggg cgtttccctt tgtgcgtgcg
      661 ttgatgctgg taacggtaaa cgtcatgatg aacacgaccg cgaataagac gacaatcgtc
      721 ggagctggtg cgctgtcgat aaaaaaggac agccagacac cagtcattga cactattacc
      781 gatatgccag tcgccagtag gagcgcaata tgaaagcgtt gtgtcaggag tactgcaatg
      841 gcgccaggcg caatgagcag agaaatagag agaatgatac ctactgcttt cagcgtcgcc
      901 acaatggtca gggaaaccat gcacagcagc ccataatgca gccatctcgt atgcaaaccg
      961 cttacctgcg cctgttgata atcaaagctg aacagcaaaa aatcccgcca ttttacgcta
     1021 ataacaagtg taaccagccc ggcaataatc accgtctgga ttatgtcgcc gatggttatc
     1081 ccgagcatgt cgccgaaaag aatatggtca agatgtacgt ctggtttaat tgcaatatac
     1141 aagatcagac ctgcggcaaa catgccagaa aaaacaatcc ccataactgt atcttgcttg
     1201 attcggctgt tgtctttcag gtatcccgtc gccaccgcgc agaatactcc ggcgacgaat
     1261 gcccctgtag ccagaggtaa ccccagaatc caggtaagca cgatgccagg aaaaaccgca
     1321 tgactcatcg cgtcacccat cagtgcccaa cctttcagga ccagaaagac cgacagcagc
     1381 gcacagggta tggaaactac cagtgaaatc agcagtgcgt gactcataaa ggtgaactgc
     1441 agtggctcca gtaataatgc catcatgagc gatcctccag cgctttatgc gcacgccgac
     1501 gatttgccaa aagtccatgg gtgggcgcaa atacaaatgc taaaagaaac agcagtgtct
     1561 gcgccaccac gatgattcca cctgtcgcgc cgtccagaaa gtaactgacc cacgcgccga
     1621 ggaaactggt tacactgcca atagttacag caattatgag caggcgggga aaacgatcgg
     1681 ttaacaacca tgctgttgca ccgggagtca ccaccagaca gatcaccaga aaggcaccaa
     1741 ccgtttgcag cgccgcaacg gtcgaaaccg acagcagggt gaagaagagg atttttaatc
     1801 tgcccggatg taatccaata gcacgggcgt gattctcgtc gaaaaaggtc accataagat
     1861 ctttccattt gaaaaaaagc acgatgatcg agagaatgcc aatgatagtg agttgcagga
     1921 tatccgccgg atcgattgcc agaatattgc ccaggacgat ggtctgaatg ttcacggatg
     1981 tcgggttcaa tgacaccata aacagcccca ggccaaaaaa ggacgagaag atcaggccga
     2041 tgatggcatc ttcttttaag cgggtgcgtt ggttaagaaa cagcatgctg cccgctgcca
     2101 gtccgccgga aaagaaggct cccagagaaa acggaagccc cagcatatat gcccccgcaa
     2161 cacccgggac aatagagtgc gacagcgcat cgccaatcag cgaccagcct tttagcatca
     2221 gatagcatga caggaaagcg cagagaccgc cgaccattgc cgagacccac atcgcattga
     2281 gcatatattc atagctgaag ggttccagta gcacgttcat cttgcttccc tctgcaccgc
     2341 tgacggtcga tgtgccacaa aagggcgttc atcgtcagta ataatgcttt cttccgagcc
     2401 gttaagtgtg acatggcgca gtacgccgct aaaagccagc tccaggttct cggctgtaaa
     2461 agtggtgtct gtcggcccgc ttgccaggac ggtgcctttg accatgaccg tataatcgca
     2521 aaatgtcgtg actgagccga gattgtgcgt tgagacaagc atcgttttgc cttccgcgcg
     2581 taactccctc aacaggctga tgattttcgc ttcggttttc acatcaacgc cggtaaaagg
     2641 ttcatccagc aggatcacat caccctgctg tgcaattgct ctcgccagaa agacgcgttt
     2701 cttttgtcca ccggaaagct cgccgatttg acgatggcga aaatccacca tatcgacacg
     2761 ttccagcgca tcggtgacaa tctgacgatc cctctttttg gctatacgca acatacccat
     2821 atgcccatag cggcccatca tcaccacatc ttccaccagt acaggaaatg accagtcaac
     2881 ctcttctgac tgagggacgt aggcaaccag gtttttctgt agcgcctgtc gcgtgggaat
     2941 acccagaaca gatatctttc cgctggtcag acgcacaaat cccataatcg ctttaaacaa
     3001 cgtcgatttt ccggaaccgt ttaccccaac cagagcggca atagatccac ttggtactgt
     3061 gaaggatgca tcgcgcagtg ctgtgtgccc gttacgccag gtgacagtaa catcattaac
     3121 gacaatgcct gcagattgca tcattatttc tcccttttcc cggctttaat tccctgtacc
     3181 agcgtactgg tagtaacctt aagaaggtcg atatacgtag gtaccgggcc gttttctgtg
     3241 ctcagggaat cgacatagag cacaccgccg tagtgtgcgc cggtttcacg cgcaacctga
     3301 cgcgctggtt tatcggaaat cgtactctcg ctaaagactg ccgggatatg atttttctta
     3361 actatatcaa caaccttacg tacctgctgc ggtgttcctt gttgatcggc attaatcggc
     3421 cacagataaa gctcttttag ccccaaatcg cgtgcgagat aagaaaaagc cccttcactg
     3481 gtgaccatcc atcgctgatt ctcagggagt tccgtaatct gcttacgcag gggggcaagg
     3541 gtttgggtaa tcttggcttt ataagtatcg gcattacgtt ggtaggtttg tgcatttgcc
     3601 ggatcgtatt ttatcaacgc atcacgaata ttatcgacgt aaatcagagc attatctggc
     3661 gacatccatg catggggatt aggtttgccc tcatagggtc cttcggtgat ccctactggc
     3721 gtcacacccg aagagacaat tacttctgga accccattga gatgctggta aaagcgttgg
     3781 aaccacaatt ccagattcat accattggcg agaatcagtt gtgccccctg cgcacgttta
     3841 atatcgccag gggtaggctg atactcatga atttctgcac caggcttggt tatggatgag
     3901 acttctgcag catctccagc cacgtttttg gccatatccg cgatgatggt aaatgttgta
     3961 atgaccttga atttttcagt tgcacttgcc tgaaatgcga tcgagcaggt gagggcgagg
     4021 caccccaaga gcatggttac tttttttatt gagtgcataa tttagtacct atagtgagca
     4081 aaattagaac aaacatagca acggctatgt tttatagcat aacctataca aaatgaaaat
     4141 aattatcatt aacagctttg aggcctaaaa cagggattag gttgtttcct gctggcgaca
     4201 tgatattgag gaaaaaagta gagcagctat taggctttga aatgacttta tgaggaaaat
     4261 tgttgatggc aaaaatgggg caaaaaagag tgggttatcg tagcttattg ttgtcgctga
     4321 taatgtttaa catattgaaa aataaataaa atacttatga gtcagatagt tgtgattttt
     4381 gcccttactt gttcaggttg tattgttctt tctttgtaat ttgttgattt tcttgcatta
     4441 tttcagttct ctggtactaa atggggcaaa ttgggggcaa actttgcaac tacgataacc
     4501 gcgcattcaa catggctatc tgttcgtcgt tcatgtcatc aatccacata ccgtaaattt
     4561 catacaccat ttgcgcagtt tcatgcccca tctggctggc tataaatgcc gggttcgctc
     4621 ctgccgtcaa cagccagcag gcaaaagtat gtcgcgtatg gtacggatta cggcggcgaa
     4681 taccagcacg ttttactgct gcattccatc ttgtacccaa actgcttacc gagtaataag
     4741 gtttctgttt ttcgttacac accctgggca tgaaaacaaa atgcagtttt tgtttttcag
     4801 ttctgccgta ctcccgatga taaaaagtga tttcgctttt gcgatgatgc ccggtcagtt
     4861 tgtattgctc cttcagtgct tcaagagccg gctgcagtaa tgctaccgtc cggatcccgg
     4921 catttgtttt tgggggaccg aacatatcaa gtatcgtcag gtttcttctg acattcacaa
     4981 ttcccttctc gaaatccaca tcctcccacg ccagagctgc cagttccccg tgacgaagcc
     5041 cggagtaaac ggcaaatttc cacaagtttt ggctctgtcc tttttcactt tccattaatg
     5101 cattgaattc tgttttagat aacgg
//
LOCUS       DAZSIO010000220         4973 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZSIO010000220 DAZSIO010000000
VERSION     DAZSIO010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.223"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACHX5T_003775"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACHX5T_003775"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFR8468771.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="ACHX5T_003776"
     CDS             1020..1703
                     /locus_tag="ACHX5T_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFR8468772.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="ACHX5T_003777"
     CDS             1700..2161
                     /locus_tag="ACHX5T_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFR8468773.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="ACHX5T_003778"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="ACHX5T_003778"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR8468774.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="ACHX5T_003779"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="ACHX5T_003779"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFR8468775.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACHX5T_003780"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACHX5T_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFR8468776.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACHX5T_003781"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACHX5T_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFR8468777.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(4903..>4973)
                     /locus_tag="ACHX5T_003782"
     CDS             complement(4903..>4973)
                     /locus_tag="ACHX5T_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468778.1"
                     /translation="NYSEYSEYTSVFFLSLMIFIKS"
BASE COUNT         1177 a         1305 c         1238 g         1253 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctgact caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg cggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tac
//
LOCUS       DAZSIO010000221         4908 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZSIO010000221 DAZSIO010000000
VERSION     DAZSIO010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.225"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            276..1661
                     /gene="dcuC"
                     /locus_tag="ACHX5T_003783"
     CDS             276..1661
                     /gene="dcuC"
                     /locus_tag="ACHX5T_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR8468779.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1702..2043)
                     /locus_tag="ACHX5T_003784"
                     /pseudo
     CDS             complement(1702..2043)
                     /locus_tag="ACHX5T_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2381..2890)
                     /locus_tag="ACHX5T_003785"
     CDS             complement(2381..2890)
                     /locus_tag="ACHX5T_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR8468780.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            3062..3298
                     /locus_tag="ACHX5T_003786"
     CDS             3062..3298
                     /locus_tag="ACHX5T_003786"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8468781.1"
                     /translation="MSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVRPWP
                     KGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            3273..4904
                     /locus_tag="ACHX5T_003787"
                     /pseudo
     CDS             3273..4904
                     /locus_tag="ACHX5T_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1222 a         1088 c         1223 g         1375 t
ORIGIN      
        1 ttagattatt ttttgagcgc acagccgcag ctaacaccat tgcaattaac aaatttgcat
       61 caatccacca tcaatttaca cacattatta tgtgataatt gccaaccgct aaatatgcgt
      121 tttgttatct atgtataaaa acagcaactt caatgtctta atggcagttt ttcttgattt
      181 taatcagcat tcatcgccaa tttattgggc atattttttc cttaagcttt aggaagtttt
      241 tattatttac tttgggcctg gagacaggaa aaattatgct gacattcatt gaactcctta
      301 ttggggttgt ggttattgtg ggtgtggctc gctacatcat taaagggtat tccgccactg
      361 gtgtgttatt tgtcggtggc ctgttattgc tgattatcag tgccattatg gggcacaaag
      421 tgttaccgtc cagccaggct tcaacaggct acagcgccac ggatatcgtt gaatacgtta
      481 aaatattact aatgagccgc ggcggcgacc tcggcatgat gattatgatg ctgtgtggct
      541 ttgccgctta catgacccat atcggcgcga atgatatggt ggtcaagctg gcgtcaaaac
      601 cattgcagta tattaactcc ccttacctgc tgatgattgc cgcctatttt gttgcttgtc
      661 tgatgtcact ggccgtctct tccgcaaccg gtctgggggt tttgctgatg gcaaccctgt
      721 ttccggtgat ggtaaacgtt ggtatcagtc gtggcgcagc agctgccatt tgtgcctccc
      781 cggcggcgat tattctcgca ccgacttcag gggatgtggt gctggcggcg caggcttccg
      841 aaatgtcgct gattgacttc gccttcaaaa cgacgctgcc tatctcaatt gctgcaatta
      901 tcggcatggc gatcgcccac ttcttctggc aacgttatct ggataaaaaa gagcacatct
      961 ctcatgaaat gttagatgtc agtgaaatca ccaccactgc ccccgcgttt tatgccattt
     1021 tgccgttcac gccgatcatc ggtgtactga tttttgacgg taaatggggt ccgcaattac
     1081 acatcatcac tattctggtg atttgtatgc tgattgcctc cattctggag ttcctccgca
     1141 gctttaatac ccagaaagtt ttctctggtc tggaagtggc ttatcgcggg atggcagatg
     1201 cgtttgctaa cgtggtgatg ctgctggttg ccgctggggt attcgctcag gggcttagca
     1261 ccatcggctt tattcaaagt ctgatttcta tcgctacctc gtttggttcg gcgagtatca
     1321 tcctgatgct ggtattggtg attctgacca tgctggcggc agtcacgacc ggttcaggca
     1381 atgcgccgtt ttatgcgttt gttgagatga tcccgaaact ggcgcactct tccggcatta
     1441 acccggcgta tttgactatt ccgatgctgc aggcgtcaaa cctgggtcgt accctatcac
     1501 ccgtttctgg cgtagtcgtt gcggttgccg ggatggcgaa aatctcacca tttgaagtcg
     1561 taaaacgcac ctcggtgccg gtgcttgttg ggctagtgat tgttatcgtt gctacagagc
     1621 tgatggtgcc aggaacggca gcagcggtca caggcaagta aatagtaatg ccggaggcaa
     1681 gtttcctccg gctttatcat gtcacccact gtggtatatg cgttgtggtc tgccaacttt
     1741 gccgtgaaca atttcagcaa taatcagatg gcggctggcg caatattcaa gataacgcct
     1801 ggcagtggtg cggctgatgg tcagtgcctg cgccaccgtt tctgccgtat gttgcacacc
     1861 aggctcttta aacagttttc gcaccgcgtt tagcgtcaag gggtcaatgc cggtcggcag
     1921 ttcgtctttg ggttcaccgc gagcataggc attaaacatc tcatcaattt gcttctggct
     1981 ggcgctatca atactttcca gcatatgttt acgctggcgg aaacgggtta gcgtttgccc
     2041 cagtgtattg attcacttga agtacgaaaa aaccgggaga acattggatt attcgggaac
     2101 tgatgggatt agatttggtg gggcttgcaa gcctgtagta caaattttag gcgttaacca
     2161 atgaaacgcg aaagatagta aaaaattgct tttgtttcat tgaaaaaacg aaaaacaaaa
     2221 acactataaa tcatttcaat catagcttca aaaaacgttc aaaaccgata acaaccaggc
     2281 tgtcatcaaa tgactcatat cacaaatcag ctcatgccgt ttaggtatgt tacatgtgtg
     2341 attatgtgag gtgaagtatg ttttagctgg ctcatggttg ttatacggct tttttcgcat
     2401 cttgtggttc caacaaagga gctacaggct cttcctgttt atgaacacca tacattgaca
     2461 aaatctcttg gcgagtttct ggaggtaatt tatgcagcgc caaagctgtt tgtagtgttt
     2521 ggcgatcaat acccagcgtt gccaataggg tgtcaacgga gatggagggg cgttcttgca
     2581 ttaatccgga aagcccatat ttagccatgt ttagctctat ctctcggact tcattttctc
     2641 ctagtttatt gattaacagg gagaacattt tcaatgtatc agcattcatt tcttcgatag
     2701 caattttttg catagccggt attgccggga tatattcaac cccaggcccg cgacgccctt
     2761 cttgtgaacg cttcggccat ccttctttct ttgctcgttt atgcaccccc tgtattgtcc
     2821 caggcattcc atccagttca gtaatttgtt tgggtgtcat ccactggttt atctcatttg
     2881 cgcttttcat aaactaagtt taccagattt atctatataa aaatcaatgg attacaaaat
     2941 aaaccacatt aactgatata aaccggtttg gtttatttgg taatgtataa aacagataat
     3001 caagacgaat tatttgtttt ggtaaccctt tatcatacgc aatatggttg gtgtgcgaaa
     3061 gatgagtaac gattggcatc gggccgacgt gattgcggcc ctcaaaaaaa caggaacccc
     3121 tctggcaaaa ttgtccagac gttcaggtta ttcaaccggg acgcttgcaa atgcattagt
     3181 caggccatgg cctaaaggtg aacgcattat tgctgacgcc ttaaacatga aacctgagga
     3241 aatttggcct agtagatatc taagggatca caatgagaga gtggatatca gcaattgaat
     3301 gcatgggttt atctggttta cctggttcat taatgggggt tcacaaaaga gctcagagag
     3361 aaggatggct caagagaaga agagaaggaa ttagaggggt tgcttacgaa taccatatat
     3421 cttctcttcc taacattgct cgagctgaag ttttgctcca acaaggaaaa tttaaaacca
     3481 gtcagggatg ttttgaaatc gcccgcccca cgctggaagc tcatgattat gatcgtgagg
     3541 cactatggag caaatgggat aaagccagcg attcccagcg cagcctggct gaaaaatggt
     3601 tgccctcaat tcaggccaca gacgaaatgc tgaaccaggg gatttcaacg aaaacggctt
     3661 ttgcgaccgt tgcagggcat taccaggtca gcgcatccac tttgcgggac aagtattacc
     3721 aggtacagaa gtttgcgaag cctgactggg cggctgcact tgttgatgga cgtggagcat
     3781 cccgtcgcaa tgttcacaaa agtgaatttg acgaggatgc ctggcagttt ctgattgcag
     3841 attatctgcg accggaaaaa cccgccttcc gcaaatgtta tgagcgtctg gaactggcag
     3901 cccgcgagca tggctggagt attccctccc gtgccacggc ctttcgccgg attcagcaac
     3961 tggacgaggc aatggttgtt gcctgtcgtg aaggtgaaca tgcactgatg catctgatac
     4021 cggcacagca gcgaactgtg gaacacctgg acgccatgca gtggatcaac ggcgacggtt
     4081 atctgcataa cgtctttgta cgctggttta acggtgatgt gatccgtccg aaaacatggt
     4141 tctggcagga tgtgaaaacc cgaaaaattc tgggctggcg ctgcgatgtg agcgagaaca
     4201 ttgattcaat tcgcctctcg ttcatggatg ttgtgactcg ctacagtatc ccggaggatt
     4261 ttcacatcac cattgataac acccgtggtg ctgcgaataa atggctgacg ggaggcgcgc
     4321 ccaatcgcta ccgctttaag gtaaaagagg acgatccaaa aggactgttt ttactgatgg
     4381 gtgcgaaaat gcactggaca agcgttgttg ccggtaaagg ctggggccag gcaaaacctg
     4441 ttgaacgtgc tttcggtgtt ggtgggcttg aggaatacgt tgataagcat ccggcactgg
     4501 ctggcgcata tacggggcca aatccgcagg caaaacctga taactatggc gaccacgctg
     4561 ttgatgcaga gctgtttctg aaaacctttg ccgaaggtgt ggcgatgttc aatgccagaa
     4621 caggccgtga aacagaaatg tgcgggggca aactctcgtt tgatgatgtt ttcgagcgtg
     4681 aatacgccag aacgattgtg cgtaagccaa ccgaagaaca aaaacggatg ctgttactgc
     4741 ctgccgaggc ggtgaacgtt tcacgcaaag gcgagtttac gcttaaagtt ggcggctccc
     4801 ttaaaggcgc gaaaaacgtt tattacaaca tggcattaat gaatgccgga gtgaaaaaag
     4861 ttgtggtcag atttgatcca cagcagttac acagcacggt ttattgag
//
LOCUS       DAZSIO010000222         4879 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZSIO010000222 DAZSIO010000000
VERSION     DAZSIO010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.226"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            192..920
                     /gene="gadW"
                     /locus_tag="ACHX5T_003788"
     CDS             192..920
                     /gene="gadW"
                     /locus_tag="ACHX5T_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR8468782.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1283..4396)
                     /gene="mdtF"
                     /locus_tag="ACHX5T_003789"
                     /pseudo
     CDS             complement(1283..4396)
                     /gene="mdtF"
                     /locus_tag="ACHX5T_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(4421..4678)
                     /locus_tag="ACHX5T_003790"
                     /pseudo
     CDS             complement(4421..4678)
                     /locus_tag="ACHX5T_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1249 a         1201 c         1192 g         1237 t
ORIGIN      
        1 agctatacgc tgtgcgaaaa tatattcgtg ctgcatttac ttattatcaa ttaactgtta
       61 tgcaaaacta ctttgtggat aaattttggt cctaccaaat ctggcagttt ttgcgctaag
      121 aaacagtctg tcatcatttc attagtatac tgaaattgaa ataatcgcag tatgaaatat
      181 aagggataat catgactcat gtctgctcgg tgatcctcat tcgtcgttca ttcgatattt
      241 atcatgaaca gcaaaaaata tcgctgcata acgagagtat cctgctgctg gagaaaaatt
      301 tggcagacga ttttgcgttt tgttcaccgg atacgcgacg actggatatc gatgagctga
      361 cagtttgcca ttacttacaa aatattcgtc agctaccacg caatttaggg ttacatagca
      421 aagaccgtct gttaattaac cagtcacccc ccatgccgct ggtgacggcg atttttgata
      481 gcttcaatga atccggggta aattcaccga tactgagcaa tatgctctac ctttcctgtt
      541 tatcgatgtt ttctcataag aaagaactga tccccttact tttcaatagc atcagcactg
      601 tttcaggaaa agttgaacgc cttattagct ttgatatcgc caaacgttgg tatctgcgcg
      661 atatcgcgga aagaatgtat accagcgaga gtctcatcaa aaaaaagttg caggatgaaa
      721 atacctgttt cagtaaaata ttactcgctt ccaggatgtc gatggccaga cgattactcg
      781 agttacgtca aattcctctg catactattg cggaaaaatg tggctatagc agtacgtcgt
      841 actttataaa cacatttcga caatattatg gtgtaacgcc acatcagttt gcgcaacatt
      901 cgccaggtac cttttcctga catattttgc atttgaatat tggtcaggat ctcacacctg
      961 cttcatgtga aactcttccc tgatgatttc tgccgggcta ccggctagtt ctctttcgca
     1021 gtttgccagg cggtataggt atttttagga ggaaaagact cggcatgttt gggattatta
     1081 agctgacaat tcatacgatt acgggaatgt gggtcagtat tgtgttgttc aaattgatga
     1141 cgaatggctg gagtggtttc tactttcagt gctgtgtact aagcctggta ttcctgacgg
     1201 ttagctggtt gttgagtggt gaatggttag caggaaagag taaggctgaa ccttcacgtt
     1261 caaccttact ctcatttaca cgttacgctt ttttaaagcg ggcaaagaga tgttccacta
     1321 caacgaaaaa gaccggaacg aagtaaattg ccagcactgt tgcggcaaac atcccgccca
     1381 tcacgccggt acctaccgcg ttttgcgcgc cagaaccggc accatgactg ataaccagcg
     1441 gcagcacgcc gagaataaag gccagagagg tcatcaggat tgggcgtaaa cgcatccgcg
     1501 ccgcttcgat gattgcctct atcggcgttt tcccttcttt ctgcatcatc tcaacggcaa
     1561 attcgacaat caggatggcg ttcttggcgg aaagcccgat ggtggtcagc aaaccaacct
     1621 ggaagtagac gtcattactt aagccgcgca gatcggtggc cagtaatgcg ccaacgacgc
     1681 ctaacggaac aaccaacatc accgagaacg gaattgacca gctctcatag agtgcggcga
     1741 gggcgaggaa caccacgacc agtgaaatgg catacagtgc aggagcctgg tttgaggata
     1801 acgcttcctg atacgacagt ccggtccatg agtagccgac gcctgccgga agtttagcga
     1861 ccaggtctgc cataaatttc atagcgtcac cggtactttt cccggccgcc gcttcaccta
     1921 aaatctccat tgacgggatg ccgttgtagc gttccaatcg cggtgaacca taggtccatt
     1981 cggtagacga gtaggcagaa agcggtgcca tcgtgccaga ggcgttgcgt acataccatt
     2041 ggttgatgtt atccggcaac atacggaacg gcgtgcctgc ctggacatac acttttttca
     2101 cccgcccctg gttgaggaag tcgttcacgt agctgctgcc gaaggcggtg gaaattgtct
     2161 ggttgatatc agacagcgcc acacccattg cctcagcttt cgcggcgttg acgttcactt
     2221 tgaacatcgg cgtatcttcc aggccgttcg ggcgtacccc ggtgacctgg tcaggcgatt
     2281 gcgctgccag tgataacagc tcgtttcgtg cctaggttag tttttcgtgc cccaggttac
     2341 cgttgtccag cagttccata tcaaaacctg acgcggtacc cagttcagcc accgcgggta
     2401 agttgaacgg gaagacgacg gctttattga tactgcttaa cgcaatcatt gcccgctgaa
     2461 tgatcgcggt aaccgagttt tcctcaccga cacgttcaga ccacggcttg agactgataa
     2521 acgccaggcc gttgttttgc ccctggccgc tgaagccaaa gccgccaacg gtaaacaccg
     2581 actgaacatt atctttctct tttgtaagat aataatccgt cacctgttgc agtactttcg
     2641 tggtgttaac catggtggca ccggaaggta actgcgcggt ggtcataaat accccctgat
     2701 cctcttctgg taagaaagag gtcggtgtac gcaggaacag caccgccatc ccggcgcaaa
     2761 tcagcaggta gaccaccatg tagcgaccgg tacaacgcaa cagcgagcgg gtgctatcgg
     2821 tatagtgctg agttgatttt tcaaacagcg tgttgaagcg tgcgaacagg gcgttaggtt
     2881 tgtgaccgcc ttccggcgtg gctttcagaa tggtggcgca cagggcaggg gtcaggctca
     2941 ttgccacaaa tactgaaagc cgcatggagg agatcagcgt gatggagaac tggcggtaga
     3001 tctccccggt tgcaccgctc ataaaggcca tcggcataaa cactgcagaa agaacaacgg
     3061 caatcccgac cagcgcacgt tggatctgcc ccatcgattt atgcgtcgct tccttcggcg
     3121 gtagcttatc ttccgcaatg acacgctcga cgttctccac caccacgatg gcgtcatcca
     3181 ccagtaaccc tatcgccagc accatcccga acatagtcaa cgtgttgatg gtgaaaccga
     3241 ccgccgacaa gatcgcaaac gtcccgagaa taaccaccgg tacggcaatt gtcgggatga
     3301 tggtggcacg gaaattctgc aaaaacagat aaatgaccag gaagacgagg atgatagcct
     3361 caaccagtgt tttgaaaact tcctgaatag aaatttcgat aaacggcgtg gtgtcgtaag
     3421 gataaaccgt cttcagactt gcggggaaat aggctgataa gcggttcagc tcctctttga
     3481 ctgcccgcga ggtatccagg gcgtttgctc cggcagccag tttgatggcg atcccggcaa
     3541 caggtttgcc gttatagcgc gccacggtgg aataatcttc cgccccaagt tcgacgcggg
     3601 cgacatcacg cagcagcact tgcgaaccat cttgctgaac tttcaacagg attttgccaa
     3661 attcttccgg cgtttgcaga cgcgtctgca caatgatcga ggcgtttagt tgctggtctg
     3721 ccgcctgtgg catgccaccc agttgaccac cagaaatctg gttgttttgc accttaatct
     3781 gggaaataac atcggaaggt accaggttat atttattgag tttttgcggg tccagccaga
     3841 tacgcatggc gtactcggaa ccaaagagct gtacgctacc aacgcccgcg gtacggctta
     3901 gcgggtcttt gatattagac gctacatagt ccgcgatatc gtactggttg aggctgccgt
     3961 tatcagaaat aaacgccgct accatcagga tattactgct cgacttatcg acgctaatcc
     4021 cctgttgctg caccgcttca ggtaatgaag gcatagcgag ttgcagttta ttttgcacct
     4081 gaacctgtgc gatatcagga gatgccccag tctcgaaggt cagagtgata gaggcattac
     4141 ccgccgcatc actggttgaa gacatgtaca tcaggccatc aagcccattc atattttgct
     4201 caatcacctg agtgaccgag tcttctaccg tttgcgcatc ggcacccgga taggtagcgc
     4261 tgacggtaat ggtcggtggc gcaatctgcg gatactgcgc aaccggtaag ttcatgatcg
     4321 ccagaccacc tgcaagcatc ataataatgg caagtaccca ggcaaaaacc gggcgatcaa
     4381 taaaatagtt agccatgaaa gtccccttaa gcctgcaacg ttattgtttc gattcggtgc
     4441 tggcgttttc ctggctggag gaaattgctc gtgctttgat acccggacga atgcgttgca
     4501 aaccggaaac gatcacccga tcgccagcct gcaagccaga ggtgacaacc cactggtcgc
     4561 cgatggcttt gctggcttca atttcgcgta gctgcacgac atcgtcttta tccagaatga
     4621 gcgccgttgc tttaccctgg gcgttgtggg tgacgccttc ctgcggcacc agtaatataa
     4681 agcgcgcaaa attattcaag atatttttga aaatcattca agataggcta tcgcgtgtga
     4741 tagcaaaaac attcaagata aaaatgcttg ttaattaaca aatgatatct tctatatatt
     4801 tttattgttt atagatggct atttcggtaa aaatgcattt tcagaatatc aaccagcggt
     4861 aaatcgaagt aaagggagt
//
LOCUS       DAZSIO010000223         4870 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZSIO010000223 DAZSIO010000000
VERSION     DAZSIO010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.227"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..527
                     /gene="ydcF"
                     /locus_tag="ACHX5T_003791"
     CDS             <1..527
                     /gene="ydcF"
                     /locus_tag="ACHX5T_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR8468783.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            724..2163
                     /gene="aldA"
                     /locus_tag="ACHX5T_003792"
     CDS             724..2163
                     /gene="aldA"
                     /locus_tag="ACHX5T_003792"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFR8468784.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2205..3205)
                     /gene="gap"
                     /locus_tag="ACHX5T_003793"
                     /pseudo
     CDS             complement(2205..3205)
                     /gene="gap"
                     /locus_tag="ACHX5T_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3394..3924
                     /gene="cybB"
                     /locus_tag="ACHX5T_003794"
     CDS             3394..3924
                     /gene="cybB"
                     /locus_tag="ACHX5T_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR8468785.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4169..4342
                     /locus_tag="ACHX5T_003795"
     CDS             4169..4342
                     /locus_tag="ACHX5T_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468786.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            4368..4436
                     /gene="yncP"
                     /locus_tag="ACHX5T_003796"
     CDS             4368..4436
                     /gene="yncP"
                     /locus_tag="ACHX5T_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR8468787.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="ACHX5T_003797"
     CDS             complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="ACHX5T_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR8468788.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4664..4804)
                     /locus_tag="ACHX5T_003798"
                     /pseudo
     CDS             complement(4664..4804)
                     /locus_tag="ACHX5T_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1247 a         1120 c         1208 g         1295 t
ORIGIN      
        1 gcaccactgg cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata
       61 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
      121 ttagcatcac gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg
      181 accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg
      241 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
      301 gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac
      361 tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag
      421 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
      481 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
      541 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
      601 tttacacttg tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg
      661 atgattgatg ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg
      721 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
      781 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc
      841 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
      901 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc
      961 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     1021 ctagctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca
     1081 cggcgttacg agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt
     1141 aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
     1201 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     1261 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     1321 ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     1381 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     1441 actgcggcga aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc
     1501 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     1561 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     1621 cagttcgtca atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa
     1681 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa
     1741 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
     1801 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     1861 atgcatgagg aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat
     1921 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     1981 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     2041 gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc
     2101 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     2161 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     2221 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
     2281 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact
     2341 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     2401 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     2461 ttccgagaat ggacaccagc tcagtgaccg aacctgtttt caccggcacg cgttgcgcat
     2521 gacctttcag tttgccgctc agttccggga tcaccagacc aatggctttt gccgcccccg
     2581 tagtgtgggg aatgatattt tctgccgctg cgcgtgaagc acgtaaatct ttaccacgcg
     2641 ggccatccac cagtgactgg gtgccagtat aggcatgaat ggtcgtcatc gtgccgactt
     2701 ctatcccgaa actgtcatgc aaggctttgg ccatcggcgc aaggcagtta gtggtgcatg
     2761 acgccacgga aacaatggtg tcgttgccat ccagagtgtc gtcattaacg ttataaacga
     2821 tggttttcat ttcaccggca ggagcggaaa tcaacacctt cttcgcacca gcatcaagat
     2881 gcgcctgcga tttatcggcg gaggtataaa agccagtaca ttcgacaatg atttctgccc
     2941 ctttcgcttt ccacggaata tttttagcct ctttttcggc gtaaaccgcg atacttttcc
     3001 catcaacgat aagtgaatct tccgtaaaat caacgctcca ggggaacgga ccatagtttg
     3061 aatcatgttt cagcagatag gcgagaattt ttggggaagt aagatcatta atagcgacaa
     3121 cgtctatgtt gcttttgact tcaagtaaac gacgcaacac cagtcgaccg atacgaccaa
     3181 aaccgttaat accaacttta ctcatggttt tctcctgtca ggaacgttcg gatgaaaatt
     3241 gatcctttcc aggcttagac caggattgtg ggatgggcaa tctccattct tacagtgaaa
     3301 cgtaacgtac tgaaaacggg tgaacaatat ttaatgaaat tttgagaaaa gcccgttatg
     3361 ttaacggaaa attatgttaa agcaggaaat gttatggaaa ataaatactc aaggttacaa
     3421 atcagcattc actggctggt ctttttgctg gttatcgcag cgtattgcgc aatggagttt
     3481 cgtggtttct tcccacgtag cgatcggccg ctcatcaaca tgattcatgt ttcctgtggc
     3541 atctcaattc tcgtgctgat ggtggttcgt cttttgttaa gactgaaata cccaaccccg
     3601 ccgattatac ctaagccgaa accgatgatg acgggactgg cgcatttggg acatttggtg
     3661 atttatctcc tgtttattgc gctgccagtg attggtttgg tgatgatgta taaccggggc
     3721 aacccgtggt ttgcgtttgg tttgacgatg ccttacgctt cagaggccaa tttcgaacgg
     3781 gttgatagct taaagtcgtg gcatgagacg ctggcgaatc tgggatattt tgtcatcggg
     3841 ttgcacgctg cggctgcact ggcacaccac tatttctgga aggacaacac acttctacgc
     3901 atgatgccgc gtaaacgttc ctgaaggata tttaaagaaa acgcctgtac taaaaccgac
     3961 ccgtggtaca ggcgaagaat acgggtctac atcggaagcg cctattatat ttatttgtat
     4021 gacaaataaa accataatcc ttgcccatac gtccatctgg cttattttta atcaattcac
     4081 ccgatctttg atctcatcaa cggtatcaaa ataaaccagc gtataacgtt caaatcgcac
     4141 ataagattcg ataaaccatg gaggttatat gaaaaaactg gcacttattt tgtttatggg
     4201 aacgcttgtt tccttttatg ccgatgccgg gcgcaaaccc tgttctggtt cgaaaggggg
     4261 gatctcacac tgtacggcag gcggcaaatt tgtctgtaat gatggttcta ttagtgcatc
     4321 gaaaaaaaca tgcactaact gaagtgtaaa aggggtgcca tgagaaaatg aatctgctag
     4381 tcagatgcgc ggggaaaatc cccgcgcttg cccttacctg gacgtgcagg ccatgagcgc
     4441 agcaacctcc ttatcaccgt cccggaaccg cagttcgtag agcgtttgtc gggtcaggag
     4501 tgtgaatgcc agaatcgtaa tgcagataat gagcagacac accaccagag ggttgcgctt
     4561 catagccttt ctccttgccg gatggcgggt aagaggctaa caatctgaat tgcttggttc
     4621 attcgttggc ctcggtttgt actgacccca aaaagttgga cagttaaaca cgaggcatat
     4681 aggtctgatt ccgatattca tttggagtca gaccttttaa tttcaggcta attcttctgc
     4741 tgttgtagta ttcaatatat tccgtaacag catccttcag ttcgcttata ttactgaact
     4801 cattagactg gccccctgaa tctccagaca accagtatca cttatttaag tgatagtctt
     4861 aatactagtt
//
LOCUS       DAZSIO010000224         4858 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZSIO010000224 DAZSIO010000000
VERSION     DAZSIO010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.228"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..1476
                     /locus_tag="ACHX5T_003799"
     CDS             <1..1476
                     /locus_tag="ACHX5T_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468789.1"
                     /translation="YHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGFNKED
                     FHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELEKVLR
                     NIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLYDFTS
                     FDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKNGCDD
                     AYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGLFGEL
                     PSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPVYKEG
                     EIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILIDKEY
                     VYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKSPVDE
                     IKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            2851..3414
                     /locus_tag="ACHX5T_003800"
     CDS             2851..3414
                     /locus_tag="ACHX5T_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFR8468790.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            3521..3867
                     /locus_tag="ACHX5T_003801"
                     /pseudo
     CDS             3521..3867
                     /locus_tag="ACHX5T_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            3931..4493
                     /locus_tag="ACHX5T_003802"
                     /pseudo
     CDS             3931..4493
                     /locus_tag="ACHX5T_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            4521..>4858
                     /locus_tag="ACHX5T_003803"
     CDS             4521..>4858
                     /locus_tag="ACHX5T_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFR8468791.1"
                     /translation="MYTESNIKLSCNALWQKCVLATLYRAAATHPKIKLLQFKLIPCP
                     GMNKAIPSLIADFTGELHRLISEHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLM
                     VLLLNNDVWC"
BASE COUNT         1414 a          843 c         1062 g         1539 t
ORIGIN      
        1 tatcatattt ataatttttt acagaaatcg tattgtcctt gtcttgatca cgctgataca
       61 gaaagcgatg aagctgctgt taaacgttat cttcgggaat atctggctga actgggattc
      121 aacaaggaag attttcatga aaatggaaag ctgtatgctc tggggaaata tacaggaact
      181 atacggaagg ataacgggaa aagcaagagc ctgatgcaac aatatatcat ggctatcaaa
      241 aatgaatata aaaaggatta ccgggagaag aagctggata aggatgaact ggagaaagtg
      301 ctcaggaata ttgataaatt ctataatgca ttttattcat taagtgtctt acttgatata
      361 caacgaaaga cgaagatact tcaaagcctt gcatattatc tcagagtgtt ggtgagagaa
      421 ggactctgga ttcatggttt gtatggttac gctgcgcaat atctttatga ctttaccagc
      481 tttgatacaa cgccttatgc aaaaaaatta ctggaaatgt tttataagtt tcaaaacagt
      541 gcagaaggaa cattgtctcg ttatagtgtt tcacttgatg ataaatctca ggaatatatt
      601 tcccgtctga aagatcttgt atttaatatt aatgataaga atggttgtga tgacgcatat
      661 ctgaaaaaga ttataagcta ttttgggcag ttgcagaatg aggctgttca tgtgaccagc
      721 tgctacgaaa cacttgcggt gtacatttgt ctgataagga aaaataaaat taatgatgtg
      781 ctgcagcatt atgatgacat ggaaagaaaa ggattgtttg gtgaattacc ttcaggatat
      841 gtcagggggg cattatcgtt gcttcggaca gcactggagg ttaaagtaaa tagaaaaaat
      901 ataaagtatg gctcgttgtt ttattggctt gaccatgtaa aagcctatca ggatgcattt
      961 attgaaaaaa taccacttat tgatcctgta tataaggagg gtgaaataca gtatgatgcg
     1021 aacaatttta ccctgatgcg tgttattaag atgtataatt gtatgcttga aaagatcagt
     1081 acgaaaccat atattgcgcc cccatatatt acaggtttgc ttgatgacgt agagaaggtg
     1141 ctggataaga taaatattct gatagataaa gagtatgttt atgatggtaa gacgctggca
     1201 gaagtcatta tggaaaacaa agtattatcc agtcgcgaac gtaaagaaac tatgataggg
     1261 cttttcacag gcagtaagaa atatacttta ctgcaatgtg ttgagaagct tggtgtatta
     1321 gttcactatg tgaaatcacc ggtcgatgaa attaaaaatg tgatgatgtt gtatggcgat
     1381 aaggccgaaa acaggaacag acgcagaatg atatatgatg cgttaaccat tatttgtgaa
     1441 gatgacatca ggaataaccc tcctgagctg tcgtgaattc atctgagtgt gttttgggtc
     1501 aataatcttt atataccctg ctaattttgc taaccattag caaaattagc aggggtatta
     1561 tctatgtctg gcaagagaaa attctataat agtatgattt atttgtttgt tttgtttttc
     1621 aattagaatg gtgtgttttt gtgatctatg tctaattgtt ttgtattctt agcgccatcc
     1681 cattcatggg cagatgtaca taccgcccct attagattga tgggttgcat gttcattgta
     1741 tctgtaaaat atgccagttt tgacattaat acataatctg taattcggtg taagctcttg
     1801 tctggtgaat tatgttgcgt attccgctct attcgatcac ggattgggcc aaatcatgat
     1861 cgattcgaat gccaaactgc gatcgattca gatgccattt tgcattatgt tagtggctta
     1921 ttcttcttgt tcttttttac aggttatgta agtgtgatta tcctgattac tggtcactca
     1981 ttatatactg agtcgacaaa acaaatgagt attaactgaa gtttttatac agtgatcatc
     2041 tgtctggtta ggttagttag ttccgggaga atcacattta tttcatatac cataaataat
     2101 taaggttaat tagtattatt agttggagtt atggtattgt gtgtctgtgc cgtattgttt
     2161 gtatgtttgt tgtataactt atctctttac gtggtaatgt cactcctctt tatgctaagt
     2221 attctgtatg tgtgtaatgc atatatctcc atacactacg cagtgaagca tatgggggaa
     2281 gctgtatggt gaattatgtg tgtaatgttg tcctgtacgt acttaacact ggttacgtat
     2341 cggaacacta ttagagcgtg ttatgactcc gttacagttt ataggtatag gtaaatatga
     2401 tcttttctgt atggaacaat cttcttatgg tgtgtgggta tgttctgtat accattattc
     2461 ttttatgcta atgattaaat acccgtggtc tctctcggct ttacttaatg gttcgacaat
     2521 attaatagcg atgttgcctg tagctagccg atactgagtt ttgtgaatgt cagattactt
     2581 cctgtaaaga atttagtggt gcttcctgaa actcaattct atgaggtgtg tttttccatt
     2641 gtaattcgta ctggttctca gtttaagaga gtctcactgt attctgaagt attacctcat
     2701 cacagtaatg cggtaataca aaacagtttt atcaggttgt ctgtgctgct ggtttacttc
     2761 gtttattaat gccgtggtac ggcgtttctc tactctcagt atttacgtaa ttctctcttc
     2821 ctgtgagttt tataaagtaa ggactataaa atgtataatg ccaatcccaa ttacaaaatg
     2881 gatttcatga ttctgaaaga cgtgaatgaa cacatggatg gtatgtttca acgtttttct
     2941 aaactgttac ctttcagaat tgattttgct tacaggaaag acacgcccag tttcgggcat
     3001 tcctgtaagc acagtatgtg tatggaaata taccgtttac ttagcgaaac ccagacgatg
     3061 ttggcgggat attactgggt aatggaatat acaccagaca aagggcttca tattcatttt
     3121 atcggctacc tggatggaca acgtcacaag aactcttatc agatatccag gcaactggga
     3181 gatatctgga ggcggatcac ggaaggggag ggttattttc atctgtgccg ggctaaagac
     3241 aaatacccgg tgcgtatcga tcacgtgatt cactatagtg ataaatctgc ggtagacgac
     3301 ttacgttacg cccttagcta tctggctaaa caggatcaga aagaacacgg tattattctg
     3361 ggccgtagca ggcttcccga aaaaagtaac cggggtcgac cacgacataa ctgaattatg
     3421 ggggcttatg tccgaactat cgtaagttat cgcgtggatt tgccttcaca cgaatttcag
     3481 tcgtggtgcc gccgggagac aggtacaagt gataacgcat atggtttata aatctgacag
     3541 cgatgaacta gcagtactgc gggcagaaaa tacccggctg gtctcattac ttgaagctca
     3601 tgggattgaa tggcgacgta aaccgcagag ccctgtgcag tgcgttttcg tattatccac
     3661 ggatgagaaa gttgcgttat ttcgtcggtt gtttcgtggg cgtgatgatg tatgggccct
     3721 tcgataggaa agcaaaacca gcggcaaatc agggtattcg tctacctggc taacgaatgg
     3781 ctgttgggaa tatgcagtaa gccctggata aaatgtgggg actgcgctca ccgtcagttg
     3841 atccctgtat ctgatctcat catctacctg aggtagtgta caatatgccg atatcgaact
     3901 gatacttaat taaaatatct agagtgtgat atatctctga gaactatcaa tacgaatatt
     3961 aaaaacactt ccagaataac agcaatacgg ggggatctcc attaccctcc tgtgattgac
     4021 aacggggata tcattacctg tttccctaac ctgatccccg gagagatatc tcgtttccgt
     4081 aactcgctga atgcgaaaat taatgcagat caacaccgta aaaaacaaca aggcaaacgc
     4141 acctacgcaa cgtcggtttg tattatctgg gcgaaagaat atacgcagtc ctttaaatgt
     4201 cattatcacg tctgcctttt tttaacaaag acgcctatta ccatctggga aactatgagc
     4261 gctctgacac cctgagaggg ttaattaccg gagcctggta cagtgcctta gggttggaac
     4321 tggaagatca ccagggatta gttcatttcc ctgctaatgg acgttacatc ctggatatta
     4381 acgatcctaa tttcccagag cagtaccagc gcttattggt acgtctggat tatttgacga
     4441 agctcgacac taaagcatca ggccagcgta actttggcta cagcggtttt tgacctattg
     4501 taactataag gtgatttatt atgtatacag aatcaaacat taagctttcc tgtaacgccc
     4561 tctggcaaaa gtgtgtcctt gcaactctgt atcgggctgc tgctacacat cctaaaatta
     4621 aattattaca gtttaaatta ataccttgcc ctggtatgaa taaggcaatt ccttctctga
     4681 ttgctgattt tactggtgag ctgcatagac taatttctga acatttgcag tgtaagcgac
     4741 gtaaagataa atcagtcgag cccacccctt tatattacct ctggagtagg gaagctgtgg
     4801 ctaacgatgc cactccttat cttatggtgc tgctacttaa taacgatgtc tggtgtaa
//
LOCUS       DAZSIO010000225         4797 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZSIO010000225 DAZSIO010000000
VERSION     DAZSIO010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.229"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..213)
                     /locus_tag="ACHX5T_003804"
     CDS             complement(<1..213)
                     /locus_tag="ACHX5T_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HFR8468792.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="ACHX5T_003805"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="ACHX5T_003805"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR8468793.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="ACHX5T_003806"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="ACHX5T_003806"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR8468794.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="ACHX5T_003807"
     CDS             2894..3634
                     /locus_tag="ACHX5T_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR8468795.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="ACHX5T_003808"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="ACHX5T_003808"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR8468796.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4192..4794)
                     /locus_tag="ACHX5T_003809"
     CDS             complement(4192..4794)
                     /locus_tag="ACHX5T_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR8468797.1"
                     /translation="MNINYPAEYDIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1207 a         1177 c         1311 g         1102 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcaa cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cagcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagat atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggttaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtcct acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctga cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tccggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga
     4441 acagaactgg cgcgaagatt cgtatttaaa acccgtgtgg taaagtttgc gtagccggga
     4501 gggaacctgt tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt
     4561 tatagcatag cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga
     4621 aaggggaacc cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc
     4681 gacgtgatta ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat
     4741 acatgtaaag acgatatctc caatatcata ttcagcaggg taattaatat tcacgtt
//
LOCUS       DAZSIO010000226         4774 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZSIO010000226 DAZSIO010000000
VERSION     DAZSIO010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.230"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            245..586
                     /gene="raiA"
                     /locus_tag="ACHX5T_003810"
     CDS             245..586
                     /gene="raiA"
                     /locus_tag="ACHX5T_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR8468798.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    689..814
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            690..737
                     /gene="pheL"
                     /locus_tag="ACHX5T_003811"
     CDS             690..737
                     /gene="pheL"
                     /locus_tag="ACHX5T_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HFR8468799.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            836..1996
                     /gene="pheA"
                     /locus_tag="ACHX5T_003812"
     CDS             836..1996
                     /gene="pheA"
                     /locus_tag="ACHX5T_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR8468800.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="ACHX5T_003813"
     CDS             complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="ACHX5T_003813"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR8468801.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="ACHX5T_003814"
     CDS             complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="ACHX5T_003814"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR8468802.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4451..>4774
                     /locus_tag="ACHX5T_003815"
     CDS             4451..>4774
                     /locus_tag="ACHX5T_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR8468803.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQ"
BASE COUNT         1295 a         1229 c         1086 g         1164 t
ORIGIN      
        1 cccttgagct gccgtttttt tattctgtta gttgtgaaac tgaagcgatt tagtcactat
       61 cgatctcatc aaatatggct cgctttgaga tattcctcaa gtaaaaaaac acctcttcct
      121 gcgatttctc acaaaaaaga ttcgttgaca aaaagtgaca aaattatgag atttccatca
      181 cacattttga catcaggaac ggtatgctga attcaccaag acgggaagac aagaggtaaa
      241 atttatgaca atgaacatta ccagcaaaca aatggaaatt actccggcca tccgccaaca
      301 tgtcgcagac cgtctcgcca aactggaaaa atggcaaaca catctgatta atccacatat
      361 cattctgtcc aaagagccac aagggtttgt tgctgacgcc acaatcaata cacctaacgg
      421 cgttctggtt gccagtggta aacatgaaga tatgtacacc gcaattaacg aattgatcaa
      481 caagctggaa cggcagctca ataaactgca gcacaaaggc gaagcacgtc gtgccgcaac
      541 atcggtgaaa gacgccaact tcgtcgaaga agttgaagaa gagtagtcct ttatattgag
      601 tgtatcgcca acgcgccttc gggcgcgttt tttgttgaca gcgtgaaaac agtacgggta
      661 ctgtactaaa gtcacttaag gaaacaaaca tgaaacacat accgtttttc ttcgcattct
      721 tttttacctt cccctgaatg ggaggcgttt cgtcgtgtga aacagaatgc gaagacgaac
      781 aataaggcct cccaaatcgg ggggcctttt ttattgataa caaaaaggca acactatgac
      841 atcggaaaac ccgttactgg cgctgcgaga gaaaatcagc gcgctggatg aaaaattatt
      901 agcgttactg gcagaacggc gcgaactggc cgtcgaggtg ggaaaagcca aactgctctc
      961 gcatcgcccg gtacgtgata ttgatcgtga acgcgatttg ctggaaagat taattacgct
     1021 cggtaaagcg caccatctgg acgcccatta cattactcgc ctgttccagc tcatcattga
     1081 agattccgta ttaactcagc aggctttgct ccaacaacat ctcaataaaa ttaatccgca
     1141 ctcagcacgc atcgcttttc tcggccccaa aggttcttat tcccatcttg cggcgcgcca
     1201 gtatgctgcc cgtcactttg agcaattcat tgaaagtggc tgcgccaaat ttgccgatat
     1261 ttttaatcag gtggaaaccg gccaggccga ctatgccgtc gtaccgattg aaaataccag
     1321 ctccggtgcc ataaacgacg tttacgatct gctgcaacat accagcttgt cgattgttgg
     1381 cgagatgacg ttaactatcg accattgttt gttggtctcc ggcactactg atttatccac
     1441 catcaatacg gtctacagcc atccgcagcc attccagcaa tgcagcaaat tccttaatcg
     1501 ttatccgcac tggaagattg aatataccga aagtacgtct gcggcaatgg aaaaggttgc
     1561 acaggcaaaa tcaccgcatg ttgctgcgtt gggaagcgaa gctggcggca ctttgtacgg
     1621 tttgcaggta ctggagcgta ttgaagcaaa tcagcgacaa aacttcaccc gatttgtggt
     1681 gttggcgcgt aaagccatta acgtgtctga tcaggttccg gcgaaaacca cgttgttaat
     1741 ggcgaccggg caacaagccg gtgcgttggt tgaagcgttg ctggtactgc gcaaccacaa
     1801 tctgattatg acccgtctgg aatcacgccc gattcacggt aatccatggg aagagatgtt
     1861 ctatctggat attcaggcta atcttgaatc agcggaaatg caaaaagcat tgaaagagtt
     1921 aggggaaatc acccgttcaa tgaaggtatt gggctgttac ccaagtgaga acgtagtgcc
     1981 tgttgatcca acctgatgaa aaggtgccgg atgatgtgaa tcatccggca ctggattatt
     2041 actggcgatt gtcattcgcc tgacgcaata acacgcggct ttcactctga aaacgctgtg
     2101 cgtaatcgcc gaaccagtgc tccaccttgc ggaaactgtc aataaacgcc tgcttatcgc
     2161 cctgctccag caactcaatc gcctcgccga aacgcttata gtaacgtttg attaacgcca
     2221 gattacgctc tgacgacata atgatgtcgg cataaagctg cggatcctga gcaaacagtc
     2281 gcccgaccat cgccagctca aggcggtaaa tcggcgaaga gagcgccaga agttgctcaa
     2341 gctgaacatt ttcttctgcc aggtgcagcc cgtaagcaaa agtagcaaag tggcgcagag
     2401 cctgaataaa cgccatattc tgatcgtgct cgacagcgct aatacgatgc aaccgagcgc
     2461 cccagacctg aatttgctcc agaaaccatt ggtatgcttc cggcttacgt ccatcacacc
     2521 agaccacaac ttgctttgcc aggctaccgc tgtccgggcc gaacatcggg tgtaacccca
     2581 gtaccgggcc atcgtgcgcc gccagcatgg cctgtaatgg tccatttttc actgatgcca
     2641 gatcaaccag aatacaatct ttcggtaaag gcggtaattt gccgataact tgctcagtaa
     2701 cgtggattgg cacactaaca atcaccattc cggcatcggc aacaatatca gccgctcgat
     2761 cccagtcatg ttgctccaga atccgcacct gataacccga tagtgtcagc atcttctcga
     2821 acaggcgtcc catctgaccg ccaccgccga cgataaccac cggacgcagt gacggacaaa
     2881 gtgttttaaa tcctttgtcg ttttcactgg agtaagattc acgcatcacc cgacgcaaaa
     2941 catcctcaat cagatctggc ggtacaccca gagcttccgc ctctgcacga cgcgaggcca
     3001 acatagatgc ctcgcgctcc ggaacataaa taggcagtcc aaagcggctt ttcaccttgc
     3061 ccacttcagc aaccagttcc agacgcttcg ctaataaatt cagcagcgct ttatcgactt
     3121 catcaatttg atcgcgtaat gcggtcaatt cagcaaccat aataaacctc ttaagccacg
     3181 cgagccgtca gctgcccgtt cagatcctga tgaatttcac gcagcaaggc atcggtcatt
     3241 tcccagctaa tgcaggcatc ggttacggat acaccgtatt tcatttcact gcgcggttgc
     3301 tcagaagact gattgccctc gtggatatta ctttcgatca tcagaccaat aattgagcga
     3361 ttaccatctt tgatttgagc aaccacggat tctgccaccg caggctgacg gcgataatct
     3421 ttattggaat taccgtggct gcaatctacc atcagagacg ggcgcagtcc cgcctgttcc
     3481 atctcttttt cacattgcgc aacatccgca gggctatagt tcggcgcttt accaccgcgc
     3541 aggatcacat gcccgtccgg attcccctga gtttgtagca acgcaacctg ccctgcctgg
     3601 ttaatgccaa caaaacagtg cggctgggcg gcggcgcgca tagcgttaat tgctgttgcc
     3661 agactgccgt cggtgccgtt tttaaaacca accggcatgg aaagcccgga ggccatttca
     3721 cggtgagttt gcgattccgt tgtacgagca ccaattgctg accagctaaa cagatcgccc
     3781 aggtattgcg ggctattcgg atctaacgct tccgtcgcca gtggcagtcc catattcacc
     3841 agctcaagca acaatttacg cgcgatttgc agcccggctt ctacatcaaa agagccatcc
     3901 atatggggat cgttaattaa ccctttccag ccgacagtgg tacggggttt ttcaaaatag
     3961 acgcgcatta ccagatagag gctatcgctg acctctgcgg caagagcttt aaatcgacga
     4021 gcatattcca gagcagtttc cggatcatga atggaacaag gaccacatac taccagcaga
     4081 cgaggatcgc gcccggcgat aatatctgaa atggttttac gcgagtcagc aatctgggct
     4141 tcttgttgca ggctcaatgg aaaagcggcc ttcagttgtt ccggagtcat taaaacctgt
     4201 tcgtcggtaa tatgtacgtt attcagcgcg tctttatgca tgatggcgat cctgtttatg
     4261 ctcgtttgcg atagttgctc ctcagcgagg atgacgtaac gataacacat aaagtaaagt
     4321 tttcaatcca tatttcgtac atttttattt acacaggcaa tttagtcgcg ctttcaaccc
     4381 ttgcttctgt atagataaat ttacatcccc tttaaaaaca atccgctatg ctttgaaaaa
     4441 ggagaaagaa atgatgaaaa agtttatcgc ccccttgttg gctttactgg ttagcggatg
     4501 tcagattgat ccttatactc acgcgccaac cttgaccagc accgactggt atgatgtcgg
     4561 tatggaagat gcgatatcgg gcagcgccat aaaagatgac gatgcattta gcgattcaca
     4621 ggcggatcgc ggtctatacc ttaaaggata tgccgaaggg caaaagaaaa cttgccagac
     4681 cgattttact tatgcccggg gactttccgg taaaagcttt cctgccagct gcaacaatgt
     4741 tgaaaatgcc agccaactgc atgaagtatg gcaa
//
LOCUS       DAZSIO010000227         4688 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZSIO010000227 DAZSIO010000000
VERSION     DAZSIO010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.231"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="ACHX5T_003816"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHX5T_003816"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1558)
                     /gene="garK"
                     /locus_tag="ACHX5T_003817"
     CDS             complement(413..1558)
                     /gene="garK"
                     /locus_tag="ACHX5T_003817"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR8468804.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1655..2545)
                     /gene="garR"
                     /locus_tag="ACHX5T_003818"
     CDS             complement(1655..2545)
                     /gene="garR"
                     /locus_tag="ACHX5T_003818"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR8468805.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(2575..3345)
                     /gene="garL"
                     /locus_tag="ACHX5T_003819"
                     /pseudo
     CDS             complement(2575..3345)
                     /gene="garL"
                     /locus_tag="ACHX5T_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3361..>4688)
                     /gene="garP"
                     /locus_tag="ACHX5T_003820"
     CDS             complement(3361..>4688)
                     /gene="garP"
                     /locus_tag="ACHX5T_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR8468806.1"
                     /translation="DTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEVAKE
                     LQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDM
                     FPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFSLAL
                     FSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISENGA
                     VVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQ
                     EKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLAST
                     IILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVAS
                     IVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1117 a         1362 c         1132 g         1077 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccaccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      601 tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa
      661 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      721 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      781 acgccatcag cgccgcaccc ataccgcccg cagctcctgc gccggggaca tcttttacat
      841 caacatgcag cgctttttta atgacatcgg catagtgaga gaggttattg tccagctcaa
      901 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
      961 ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1021 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1081 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1141 catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1201 gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1261 gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1321 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1381 cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaaccg
     1441 gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1501 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1561 ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg gattccaggc
     1621 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1681 gtttttcgta gtagcacacc agggcgctat gatccgccgt tcctaaacca tctgctcgca
     1741 gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgccg acgccgtggg
     1801 aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag cccggcttga
     1861 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagtccac
     1921 cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc agcgttaacg
     1981 cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt ttggtgacgt
     2041 tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc atcaaatcat
     2101 agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg
     2161 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2221 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2281 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2341 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagagg
     2401 ctgtttctgc acctgcagca atcacgtcag caatagcttc tgggttacgg tcagcaacca
     2461 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2521 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2581 tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat cgctgccgac
     2641 agccacaaac gtcgcgcccc attccagata acgacgcgca tcggcttcga ccggcgcgag
     2701 gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg
     2761 tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat cgctggggcc
     2821 gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat ctacgccctg
     2881 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     2941 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg
     3001 gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3061 gaagttatag aaaccgatat ccagaagacg cttaataatt tccggctcgt tggtcggcac
     3121 tcacactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa taaacgtgga
     3181 gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3241 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3301 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3361 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3421 catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3481 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3541 gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg gccagcccag
     3601 agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3661 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3721 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3781 cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac ccactttcag
     3841 aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     3901 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     3961 ttgcttgatg taatgcagtt tgggaccgct tgctgccgcg ctgcccggct ttttgtggtc
     4021 catatcgacc accgcgccat tttcagagat aaacttcagc tcttcctcag acatacgtgg
     4081 gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aacaaatcac
     4141 ccccataacg gtaaagacgt gctcccatcc ccaggcgaaa gtcagccagc caagcagagg
     4201 cgaaaagagc gccagcgaga aatattgtgc cgagttaaag atggcggagg cagtaccacg
     4261 ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaagg atggagcttc
     4321 cgagaatccc agcataaagc gcataaagaa catggagatc cctgcccaag ccagcgggaa
     4381 catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta
     4441 aacttttttc gagccaaact tatcaagcaa ccagccgccg gggatttgca tcagcaagta
     4501 ggcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4561 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac
     4621 aataaaaata atcagtaata ttaaatagcg ggtatgcaca cctttccttt tttcgtcaac
     4681 ggtgtcca
//
LOCUS       DAZSIO010000228         4608 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZSIO010000228 DAZSIO010000000
VERSION     DAZSIO010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.232"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..1517
                     /gene="mltF"
                     /locus_tag="ACHX5T_003821"
     CDS             <1..1517
                     /gene="mltF"
                     /locus_tag="ACHX5T_003821"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR8468807.1"
                     /translation="ALLLAVALWPSIPWFGKADNRIAAIQARGELRVSTIHTPLTYNE
                     INGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNADLLAAGLVYNSERV
                     KNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVVVNDLQTLKETKFPE
                     LSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELAVALDITDEQPVTWF
                     SPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFLRAVDAVLPQ
                     LKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLTKNTAQSLGITDRTD
                     AEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMLDARALTAKTKGNPD
                     SWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEKQATE
                     AAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN"
     gene            complement(1514..2017)
                     /gene="tadA"
                     /locus_tag="ACHX5T_003822"
     CDS             complement(1514..2017)
                     /gene="tadA"
                     /locus_tag="ACHX5T_003822"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR8468808.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(2075..2710)
                     /gene="yfhb"
                     /locus_tag="ACHX5T_003823"
     CDS             complement(2075..2710)
                     /gene="yfhb"
                     /locus_tag="ACHX5T_003823"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR8468809.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            2919..3767
                     /locus_tag="ACHX5T_003824"
     CDS             2919..3767
                     /locus_tag="ACHX5T_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR8468810.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(4106..4608)
                     /locus_tag="ACHX5T_003825"
                     /pseudo
     CDS             complement(4106..4608)
                     /locus_tag="ACHX5T_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         1185 a         1207 c         1119 g         1097 t
ORIGIN      
        1 tggcactgct gctcgcggtc gctctctggc catccattcc ctggtttggt aaagccgaca
       61 accgtatcgc cgccattcaa gcgcggggag agttgcgtgt gagcaccatt catactcccc
      121 tgacttataa cgaaatcaac gggaaacctt ttggcctgga ttacgaactg gcgaaacagt
      181 ttgccgatta cctcggcgta aaactgaaag tgaccgtgcg gcagaatatc agccagctgt
      241 ttgacgacct tgataatggt aacgccgacc tgctggcggc aggactggtc tataacagtg
      301 agcgggtaaa aaattatcag cctggcccta cctattattc cgtgtcacaa caactggttt
      361 ataaagtggg tcagtatcgc ccacgtacgc tgggcaacct gacggcggag caactcaccg
      421 ttgcaccggg tcatgtggta gttaacgatc tccagaccct gaaagaaaca aaattcccgg
      481 aattaagctg gaaggtagac gacaaaaaag gctctgcgga attaatggaa gatgtcatcg
      541 aaggaaaact cgattacacc attgctgatt ctgtcgccat cagcctgttt cagcgcgttc
      601 acccggagct cgccgtagcg ctcgatatca ccgatgaaca accggtgact tggtttagcc
      661 cgttagatgg cgataatacc ctttccgccg ccctgctcga cttcttcaac gaaatgaatg
      721 aagacggtac gctggcacgc attgaagaga aatacctggg gcatggcgat gattttgatt
      781 acgtcgatac gcgcacattt ttacgcgccg tcgatgcggt actgccgcag ttaaagcccc
      841 tgtttgagaa atacgccgaa gaaattgact ggcgtttgct ggccgctatt gcttatcagg
      901 aatcgcactg ggatgcacag gccacttcac cgacgggtgt gcgcggcatg atgatgttaa
      961 ccaaaaatac cgcgcaaagc ctcggcatta cggatcgtac cgatgccgaa cagagcatca
     1021 gcggtggcgt gcgttatttg caggatatga tgagtaaagt gccggaaagt gtgccggaga
     1081 acgaacggat ctggtttgcc ctcgctgcgt acaatatggg ctatgcgcat atgctggatg
     1141 cccgcgccct gacggcaaaa accaaaggga atcctgacag ttgggctgac gtaaaacagc
     1201 gtctgccttt acttagccag aaaccctatt acagcaagct gacttacggc tacgctcgtg
     1261 ggcatgaagc ctacgcttat gtcgaaaata ttcgtaagta tcagattagc ctggtgggtt
     1321 atctgcaaga gaaagagaag caggctacag aagcggcgat gcaactggcg caggattatc
     1381 cggcggtatc gcctacggag ttgggcaaag agaaatttcc ttttctctcg tttctttccc
     1441 agtcgtcatc aaactatttg acccattctc cctctctgct gttttccagg aaagggagtg
     1501 aagagaaaca aaattaatcc gtcgaggatt gcgctttttt ctgcgcttta atttcctggc
     1561 ggcgcatgcg aaagaagtca ctgagcaacg ccgcgcactc atccgccagt attccttccg
     1621 taatttccac tcggtgattc atacccggat gatgcagcac atccattaaa gatcccgcag
     1681 cgccagtttt cgcgtcacgc gcaccaaaga ccacgcgacc aatgcgactg tggatcatcg
     1741 ctccggcaca cattacacat ggttcaagcg tgacatacaa cgtggcgtcg atcagacgat
     1801 aattttgcat caccagacca ccctgccgca gggccatgat ttctgcatgt gcggtgggat
     1861 catggcgacc aatcgggcgg ttccagcctt cgccgattac ccgattgtta tgcactaata
     1921 ccgcgccgac cggcacttcc cgctcatccc aggcacgttt cgccagcgtc agcgcgtgac
     1981 gcatccaata ttcgtggcta aattcgactt cagacaaaaa gaaaactccg gttataaaag
     2041 cgcggcgcat tatacacgga cgctatgctt tactctattc cagttgctgg agttcaccgc
     2101 gcggggttac tcgccaacga tgctgacaga aataaagcag cggattgtcc tgattactgt
     2161 cgctatagcc actgtacagc cgcagcggcg tgccgatttt gcgctccagt tgtgcgacct
     2221 tttcatgtcc cagacaacgc atcgtcaata cccaaccacc atagccacgc tgaatttggc
     2281 tggcgataag attaacccgc ggcagccagg gcgtatcgaa ataaaccgct tcaaccagcg
     2341 gctgcggaga gccggtaatc aaccagatat cagcatcgga acttaacagg taggtggtta
     2401 atcgctcctg aaccagcgga aaggcggtaa cattgtcgcg aaaccagcgc acgaaatcgg
     2461 cctgcaacgt ctgtaaacgt gcttcgctgt gaccaaaagt gcacccccac agaagcagac
     2521 tcatcggcca gcgtgccgca cgacctttta tcaataacgc aatggctata atcggtaaca
     2581 acggcaggac aagtaacgca ttcagcggtt ggcgacgcag taaatagcgc agaaaactgc
     2641 cgaacatatc ctgctgatgc aatgttccat ctaagtcaaa aaacaccaca cgacgctcgt
     2701 gagttgccaa accatactcc tctggactga aacatcctga ttaattttcg ctactacata
     2761 gcctaacaga tagatcatca cttttccggc aacaatcgac cgcatcatgg ctaactggaa
     2821 tttttaattc atggcaatta gcggcaatgg aatataaaat tcactcgcgt gtgtctcata
     2881 ttgcctgttg tcgccactat ttcatcgcga ggaaagatat gaacggctta cttcgtatcc
     2941 gtcagcgtta ccaagggctt gcccagagtg ataaaaaact ggcggattat ctgctgctac
     3001 aacctgatac ggcgcgccat ttaagctctc agcaactggc caacgaagcc ggagtcagtc
     3061 agtccagcgt cgtgaagttc gcgcaaaaac tcggctataa aggttttccg gcgcttaagt
     3121 tggcgttgag tgaagcgctg gcaagccagc cggaatcgcc ctccgtgccc attcataacc
     3181 aaatccgcgg tgatgatccg ttacggctgg tcggcgaaaa actgattaaa gaaaataccg
     3241 ccgctatgta cgcaacgcta aacgttaata gtgaagagaa actgcatgaa tgcgtaacaa
     3301 tgttgcgctc tgcgcggcgg ataattctga ccggtattgg cgcttcgggt ctggtggcgc
     3361 aaaactttgc ctggaagctg atgaagattg gcttcaatgc tgccgcactg cgcgatatgc
     3421 atgcgctact cgcaacagta caggcgtcgt cccctgacga tctgttatta gccatttcct
     3481 acaccggtgt acgacgcgag ttaaacctgg cggcagatga gatgctgcga gtgggcggaa
     3541 aagtgctggc gattaccggc tttactccga atgccctgca acagcgtgct tctcattgcc
     3601 tgtataccat tgccgaagaa caagcgacaa acagtgcttc aatctctgct tgtcacgctc
     3661 agggaatgtt aacggatttg ctgttcattg cgctgattca gcaggatctg gaactggcac
     3721 cagaacgtat tcgtcatagt gaagcgctgg tgaaaaaact ggtctgagta aagaatgcgc
     3781 gtataatgcc cgcccggttt gtgttgtttt gagagtttcc ttatggcgct gttaatcact
     3841 aaaaaatgca tcaattgtga tatgtgtgaa atcaaataaa aacacattta ttttcatagt
     3901 gatacagcaa tgtgttattg aatgtgttgt tggatttatg gtgcaaaaaa attttgcata
     3961 agttcagcaa cacaaagaac accactttct ttaacgtcct ccgcgcccta aaggacgggg
     4021 ctttacggcg caaaaggcct gtgacataca acattaactt ttcagtcaac cgtagcacgt
     4081 tcttgtatac gccgtgtctg cggttttata taaccctggc tggaaacctc ttatacaaag
     4141 tcgattcgcc aacatcatag atgatcgcca ccttctggcg agaaactcct gatgcaatta
     4201 gtcgcccggc ctgcgcccat tattctggtg aaagtttggg acggcgaccg ccaattcgtc
     4261 cctgtgcgcg agcagcttcc agtcctgctt ttgttcgttc aacaatcagt tcacgttcca
     4321 tttcagccag ggcacccatc acatggaaga aaaagcgtcc cattggtgtg ctggtatcaa
     4381 ttgaatcagt cagactacga aagttgatgc ctcgttcgcg caacgcctcc accagcacga
     4441 caagatgccg catactgcgc cccagccgat ccagcttcca gacaaccagt gtgtcgcctg
     4501 ccgataatgt cttgagcagt tttttcagcc ccggtctttc ggactttgta ccacttatct
     4561 tgtcttcaaa aatcagctcg catcctgcac agttcaacgc attacgtt
//
LOCUS       DAZSIO010000229         4603 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZSIO010000229 DAZSIO010000000
VERSION     DAZSIO010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.233"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(79..867)
                     /gene="phoH"
                     /locus_tag="ACHX5T_003826"
     CDS             complement(79..867)
                     /gene="phoH"
                     /locus_tag="ACHX5T_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR8468811.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1474..2739)
                     /gene="efeB"
                     /locus_tag="ACHX5T_003827"
     CDS             complement(1474..2739)
                     /gene="efeB"
                     /locus_tag="ACHX5T_003827"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR8468812.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2745..3872)
                     /gene="efeO"
                     /locus_tag="ACHX5T_003828"
     CDS             complement(2745..3872)
                     /gene="efeO"
                     /locus_tag="ACHX5T_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR8468813.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3930..>4603)
                     /gene="efeU"
                     /locus_tag="ACHX5T_003829"
     CDS             complement(3930..>4603)
                     /gene="efeU"
                     /locus_tag="ACHX5T_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR8468814.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1081 a         1296 c         1109 g         1117 t
ORIGIN      
        1 tttcagaaaa agcgctagtc cgggcataaa cacccaaaac aaagcccggt tcgcccgggc
       61 tctgcaccga taatacactt aactgtatgc atgcagcgta cgttggcaaa gtgccgaacg
      121 tacgcaatcc tcttttccga accggacgat cccaaccatt tcatcttctt cgaaacgttc
      181 cagcgcgtca cttaatccgg agcacacgcc gcgaggcaaa tcgcattgcg tgatatcacc
      241 gttgacgata accgtcacgt tctccccgag gcgggttaaa aacattttca tttgcgcggc
      301 agtcacattc tgcgcctcgt caagaatgac aactgcattt tcaaaggtac gtccacgcat
      361 ataggcgaac ggcgcggttt ccaccttccc tatttccggt cgcaggcagt actgcataaa
      421 ggaagcccct aagcgccgga ccagcaagtc gtagaccggg cgaaaatagg gagcaaactt
      481 ttctgcgata tcaccaggta agaagccaag atcttcatcg gcttgcagaa ctggacgggt
      541 gacgataatc ctgtcgacat ccttatgtat cagggcctct gccgcttttg ctgcgctgat
      601 ccaggttttt ccgcacccgg cttcgcccgt ggcgaatatc agctgcttac tctcaatagc
      661 ctgcagatag tgcaattgcg cttcatttcg cgcaaggatg ggcgaggtat cgcgactgtc
      721 gcgggccata ccaatggctt ctacgccgct catctgcaca agcgaggtga ccgattcttc
      781 ttcacgctgc ttatggctgc gcgaatcccg tctcagcaca cgttttgcct cgcgacgagc
      841 tttgatcact gctttttgtc ttcccatgga gagcaccttg agttgtttgt attcatcaca
      901 cgcgccgttg gcagcgcgat tatgcgcacg aacatcagag ggttggcttc cttgtaagcc
      961 atagtttgct tttggataaa atgccgaaaa acggctacgc acaccgtttg cggcgtcggt
     1021 aacacgagta aagaaaggaa gtgagaaaaa tacagagtga cgaagttgct accggaggag
     1081 aaatctccgc gagtggtgga gcgttgctta acaaaacatt gtccagaaca ggacgttacc
     1141 atccgcgatc tccatagtga ctgactatca ctgccgggaa cttccgctgc tacttaataa
     1201 gtacaacaga actcgcattc attgcaacaa tatattcact tatatttaac gataacaccc
     1261 atttcggtga cattaatttc tactggaaag atgacagagt gatgacagtg atggaaaaag
     1321 ctgtgtgctt tccgcaggat tcgcagggct ggtaaaatgt gcctcgtgtt tgccggattc
     1381 ggcgcgaacg ccttacccga cctacaagtg attaataatt tgtgcacaaa actcatgccc
     1441 gataaacctg cgcatcgggc atatttgcgt cgttcaaacc tgcaatagcg cccttcccag
     1501 ataatcgttc gcgtccttca ctcccggcag cgaaaaaaaa taaccgccgc cgataggttt
     1561 aacgtattcc tccagcgctt cgccattgag ccttttttgt actgtcagga agcctttttc
     1621 cagatcgtgt tggtagcaga caaacagcaa ccccatatcc agttgtccgg agttggtgac
     1681 ccccaacgaa taactgtagc cgcgacgcag catcaggctg gactcactct ccgccgtgcg
     1741 gggattcgcc agccggatat ggctgtccag cgcgatcacc ttcccttccg ggtcgctggc
     1801 gtaatcaggc acatcatgct catgctgcat tcccagcggc gcaccggttt gcttatcacg
     1861 gccaaaaatc gtctgctgtt ctttcagcgg cgttctgtcc caaaattcca ctcgaaactg
     1921 aatcaagcgt actgcctgat agctgccacc gattgtccac gcaggctcct gctgatctgc
     1981 cgttacccac accacttttt gcatcaactt atcattctgg ctatcgggat tggcagtgcc
     2041 gtctttgaaa cccagcaaat taatcggcgt ctctttgcct ttactacgcg ccgcgtgatc
     2101 ggaaataaac ccttcccgct tccagcgcac actgagcaaa tccggcgtgt gtttgatgat
     2161 atcgcgcagc gcatggttca ccgtgtcctg ggtgttggcg caaatctgta gcaacacatc
     2221 accatgacat aacgccgcat ccagcgagtc gttggggaaa cgcgtcatct tctgcagctt
     2281 ttttggcatc tgtggcgcaa ggccaaagcg ctcatcaaac aatgagtgac ccaccgataa
     2341 cgtgatggtg agattatcgg gcgcaatgta gccgccaaga atgccggaat cgagtggtgg
     2401 caggcgcgga tttggcgttt ctggtgctgc tccgccctga ctcagaaaag caaaacgctg
     2461 agtcaacaag cgaaacaacc gctcaagatc ggctttatcg ctggcaagca catcaaacgc
     2521 caccagcatc attgcggcct gttgtggcgt caggatccct gcctgatgct caccataaaa
     2581 cggctgtttc tcattgcgtg catccggtga aagcgtgccc ggtgcacttt gcgttttttg
     2641 tgcatgagcg accggacaac ttcccgccag cgcaccaatc cctttcagta aacgtcggcg
     2701 tgacggttca ttcacgccgt tttcatcttt atactgcata acgcttaatc cagtcccagc
     2761 acaccgcgaa gttgcgccag atcttccgcc agcgcagtaa tcggcccttt cagcgcattc
     2821 cggtcggcat cggtcaattt gtcgtaggtt tcaaaaccgt ctttagtacg gtatttcgcc
     2881 agaatggtat cgaccttttt aaagttggca tcgacttttg ccaacagttc cgggttggct
     2941 ttttgcagtt gtggacgcag cagatcgaca attttctgcg agccttcaac gttagcctgg
     3001 aaatcccaca gatcggtgtg gctgtagcga tcttcttcac cgctgatttt gctggctgcc
     3061 acttcctcaa tcagtccggc tgcgccgccg accacttttg aaggtgggaa agccagttca
     3121 ctgatgcgtt tttgcaaatc gaccacatcg gtataaagct gctcagcgta ctgatccatc
     3181 cctttggtgg tgttgtcgcc aaacaatgct ttttccagac ggtggaaacc agtgaatttt
     3241 gggtcggcgg ctttttgctc gtaatcatct tcacgggcgt caatgctgcc atccagatcg
     3301 gagaacagtt cagcaatcgg ttcaatacgc tcatagtgct ggcgcgtcgg cgcatacagt
     3361 gctttcgctt tttcgatatc gcctgcttta atcgcgtcgg taaaggcttt ggtgtcggtc
     3421 accagctgcg tggtttccgc catgacatac gctttatatg cagtaattgc accaccaaga
     3481 cttaacagcg catcactttg cgccgcatcc gccgttgcct cacctttgac gatcaacttc
     3541 cctttcgggt tagtcagcag accgcaggtc atatcgtatt cgccaggctg taaattcgcc
     3601 gtcattttct ggctaaaacc aggggcgata ttttcccgct cttccaccac catcacgcct
     3661 ttgaggatct cccactccag cgccttctgg ctatggttct gaataatgaa ctgtgttttc
     3721 ccggcgttaa ccgtaatggt catcggttcg cactgcttat ccgtcacggt cactttgacc
     3781 tgcggcacat cagcggcgtt agccataaaa gcagaagaaa acagcgcagc cacgctcaac
     3841 tgcaatgcgt tacggcggaa gttaatggtc atgacgagac tatcccttta aagtatgttg
     3901 taactaagca agagtttgcg tcgtatttgt tacgcggagc gagacgctgt cgcccctgcg
     3961 cgtggtggca gagcaaatgc caccagcgcc gggatgagat aaataaacca gacggcgact
     4021 tcgctgacgc tcggtgcttc ctgatagcca aaaatccctt ccatcagcgt gccaaacagc
     4081 gagtgagttg agaggaccgc gctcatatcg aaggcgattt cctgaaagtg gttccacaat
     4141 ccggcttcat gaaatgcgcg aatggcacca gccgccagcc ctgcggcgac gaagagaata
     4201 aacaggctgg tccatttaaa aaatgcacca agattgaggc gaataccgcc ccagtagagc
     4261 aggaagccga gcaccacggc agtagcaaga ccgagcattg cacccagcgg cggccagatc
     4321 ccgacatctt gttgaaatgc cgccagcagg aaaaagaccg actccagccc ttcccttgca
     4381 acggcaaaaa agaccatcat caccagcgcc cagccatgat gatttccacg ctgcaatgcg
     4441 ctatcgactg cctgttccag ttgcactttg acgttgcgcg acactttgcg catccagaaa
     4501 accatccagg taaggatcac cacggcgatc accgccacga taccttcaaa cagttcctgc
     4561 tctttttgcg gaaattcgcc ggtggtttcg ttaatgaaga tcc
//
LOCUS       DAZSIO010000230         4395 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZSIO010000230 DAZSIO010000000
VERSION     DAZSIO010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.234"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            110..967
                     /gene="focA"
                     /locus_tag="ACHX5T_003830"
     CDS             110..967
                     /gene="focA"
                     /locus_tag="ACHX5T_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR8468815.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1022..3304
                     /gene="pflB"
                     /locus_tag="ACHX5T_003831"
     CDS             1022..3304
                     /gene="pflB"
                     /locus_tag="ACHX5T_003831"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR8468816.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3496..4236
                     /gene="pflA"
                     /locus_tag="ACHX5T_003832"
     CDS             3496..4236
                     /gene="pflA"
                     /locus_tag="ACHX5T_003832"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR8468817.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4318..>4395)
                     /locus_tag="ACHX5T_003833"
     CDS             complement(4318..>4395)
                     /locus_tag="ACHX5T_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468818.1"
                     /translation="FDGEELHASYYQSLLSQVRDMVDAL"
BASE COUNT         1111 a         1096 c         1075 g         1113 t
ORIGIN      
        1 ccatgcgagt tacgggccta taagccaggc gagatatgat ctatatcaat ttctcatcta
       61 taatgctttg ttagtatctc gtcgccgact taataaagag agagttagtg tgaaagctga
      121 caaccctttt gatcttttac ttcctgctgc aatggccaaa gtggccgaag aggcgggtgt
      181 ctataaagca acgaaacatc cgcttaagac tttctatctg gcgattaccg ccggtgtttt
      241 catctcaatc gcattcgtct tctatatcac agcaaccact ggcacaggca caatgccctt
      301 cggcatggca aaactggttg gcggcatttg cttctctctg gggctgattc tttgtgttgt
      361 ctgcggagcc gatctcttta cttccacagt gttgattgtt gttgctaagg cgagtgggcg
      421 catcacctgg tgtcagttgg ctaaaaactg gctaaatgtc tattttggca acctggtcgg
      481 cgcactgctg tttgtacttt taatgtggct ttccggcgag tatatgaccg caaatggtca
      541 atggggacta aacgtcctac aaaccgccga ccacaaagtg caccatactt ttattgaggc
      601 cgtctgtctt ggtatcctgg caaacctgat ggtatgtctg gcagtatgga tgagttattc
      661 tggccgcagc ctgatggaca aagcgttcat tatggtgctg ccggtcgcga tgtttgttgc
      721 cagcggtttt gagcacagta tcgcaaacat gtttatgatc ccgatgggta ttgtaatccg
      781 cgacttcgca tctccggaat tctggaccgc tgtcggttct gcaccggaaa atttttctca
      841 cctgaccgtg atgaacttca tcactgataa cctgatcccg gttacgatcg gtaatattat
      901 cggcggtggt ttgttggttg ggttgacata ctgggtcatt tacctgcgtg aaaacgatca
      961 ccattaatgg ttgtcgaagt acgcagtaaa taaaaaatcc acttaagaag gtaggtgtta
     1021 catgtccgag cttaatgaaa agttagccac agcctgggaa ggttttacca aaggtgactg
     1081 gcagaatgaa gtaaacgtcc gtgacttcat tcagaaaaac tacactccgt acgagggtga
     1141 cgagtccttc ctggctggcg ctactgaagc gaccaccacc ctgtgggaca aagtaatgga
     1201 aggtgttaaa ctggaaaacc gcactcacgc gccagttgat tttgacaccg ctgttgcttc
     1261 caccatcacc tctcacgacg ctggctacat caataaagcg ttggaaaaag ttgttggtct
     1321 gcagactgaa gctccgctga aacgtgctct tatcccgttc ggtggtatca aaatgatcga
     1381 aggttcctgc aaagcgtaca accgcgaact ggacccgatg atcaaaaaaa tcttcactga
     1441 ataccgtaaa actcacaacc agggcgtgtt cgacgtttac actccggaca tcctgcgttg
     1501 ccgtaaatcc ggtgttctga ccggtctgcc agatgcttat ggccgtggcc gtatcatcgg
     1561 tgactaccgt cgcgttgcgc tgtacggtat cgactacctg atgaaagaca aatacgctca
     1621 gttcacctct ctacaggctg atctggaaaa cggcgtaaac ctggaacaga ctatccgtct
     1681 gcgcgaagaa atcgctgaac agcaccgcgc tctgggtcag atgaaagaaa tggctgcgaa
     1741 atacggctac gacatctctg gtccggctac caacgctcag gaagctatcc agtggactta
     1801 cttcggctac ctggctgctg ttaagtctca gaacggtgct gcaatgtcct tcggtcgtac
     1861 ctccaccttc ctggatgtgt acatcgaacg tgacctgaaa gctggcaaga tcaccgaaca
     1921 agaagcgcag gaaatggttg accacctggt catgaaactg cgtatggttc gcttcctgcg
     1981 tactccggaa tacgatgaac tgttctctgg cgacccaatc tgggcaaccg aatctatcgg
     2041 tggtatgggc ctcgatggtc gtaccctggt taccaaaaac agcttccgtt tcctgaacac
     2101 cctgtacact atggggccgt ctccggaacc gaacatgacc attctgtggt ctgaaaaact
     2161 gccgctgaac ttcaagaaat tcgccgctaa agtgtccatc gacacctctt ctctgcagta
     2221 tgagaacgat gacctgatgc gtccggactt caacaacgat gactacgcta tcgcttgctg
     2281 cgtaagcccg atgatcgttg gtaaacaaat gcagttcttc ggtgcgcgtg caaacctggc
     2341 gaaaaccatg ctgtacgcaa tcaacggcgg cgttgacgaa aaactgaaaa tgcaggttgg
     2401 tccgaagtct gaaccgatca aaggcgatct cctgagctac gatgaagtga tggagcgcat
     2461 ggatcacttc atggactggc tggctaaaca gtacatcact gcactgaaca tcatccacta
     2521 catgcacgac aagtacagct acgaagcctc tctgatggcg ctgcacgacc gtgacgttat
     2581 ccgcaccatg gcgtgtggta tcgctggtct gtccgttgct gctgactccc tgtctgcaat
     2641 caaatatgcg aaagttaaac cgattcgtga cgaagacggt ctggctatcg acttcgaaat
     2701 cgaaggcgaa tacccgcagt ttggtaacaa tgatccgcgt gtagatgacc tggctgttga
     2761 cctggtagaa cgtttcatga agaaaattca gaaactgcac acctaccgtg acgctatccc
     2821 gactcagtct gttctgacca tcacttctaa cgttgtgtat ggtaagaaaa ctggtaacac
     2881 cccagacggt cgtcgtgctg gcgcgccgtt cggaccgggt gctaacccga tgcacggtcg
     2941 tgaccagaaa ggtgctgtag cgtctctgac ttccgttgct aaactgccgt ttgcttacgc
     3001 taaagatggt atctcctaca ccttctctat cgttccgaac gcactgggta aagacgacga
     3061 agttcgtaag accaacctgg ctggtctgat ggatggttac ttccaccacg aagcatccat
     3121 cgaaggtggt cagcacctga acgttaacgt gatgaaccgt gaaatgctgc tcgacgcgat
     3181 ggaaaacccg gaaaaatatc cgcagctgac catccgtgta tctggctacg cagtacgttt
     3241 caactcgctg actaaagaac agcagcagga cgttattact cgtaccttca ctcaatctat
     3301 gtaattagat ttgactgaaa tcgtacagta aaaagcgtac aataaaggct ccacgaaagt
     3361 ggggcctttt ttagcacgag agcctttttt gtcagctatc tatactttaa ggtgactgcc
     3421 aaaacagact cgacgtagcc ttcgagctgc gcaccaacac ggcctcagat gggccacatc
     3481 tggagaaaca ccgcaatgtc agttattggt cgcattcact cctttgaatc ctgtggaacc
     3541 gtagacggcc caggtattcg ctttatcacc tttttccagg gctgcctgat gcgctgcctg
     3601 tattgtcata accgcgacac ctgggacacg catggcggta aagaagttac cgttgaagat
     3661 ttgatgaagg aagtggtgac ctatcgccac tttatgaacg cttccggcgg cggcgttacc
     3721 gcatccggcg gtgaagcaat cctgcaagct gagtttgttc gtgactggtt ccgcgcctgt
     3781 aaaaaagaag gcattcatac ctgtctggat accaacggtt ttgttcgtcg ttacgatccg
     3841 gtgattgatg aactgctgga agtaaccgac ctggtaatgc tcgatctcaa acagatgaac
     3901 gacgagatcc accaaaatct ggttggagtt tccaaccacc gcacgctgga gttcgctaaa
     3961 tatctggcga acaaaaatgt gaaggtgtgg atccgctacg ttgttgtccc aggctggtct
     4021 gacgatgacg attcggcaca tcgcctcggt gaatttaccc gtgatatggg taacgttgag
     4081 aaaatcgaac ttctccccta ccacgaactg ggcaaacaca aatgggtggc gatgggtgaa
     4141 gaatacaaac tcgatggtgt taaaccaccg aagaaagaga ccatggaacg cgtgaaaggc
     4201 attcttgagc agtacggtca taaggtaatg ttctaatttc ctcgtaaagc ggcaacttta
     4261 ttgagttgcc gcttttttat tccgcgcttc attctgctac agcgttttta acacatctta
     4321 tagtgcatct accatatccc gtacctgaga taataacgac tgataatagc tcgcgtgaag
     4381 ttcttcccca tcgaa
//
LOCUS       DAZSIO010000231         4319 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZSIO010000231 DAZSIO010000000
VERSION     DAZSIO010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.235"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            3..857
                     /gene="gatY"
                     /locus_tag="ACHX5T_003834"
     CDS             3..857
                     /gene="gatY"
                     /locus_tag="ACHX5T_003834"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR8468819.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            886..2148
                     /gene="gatZ"
                     /locus_tag="ACHX5T_003835"
     CDS             886..2148
                     /gene="gatZ"
                     /locus_tag="ACHX5T_003835"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFR8468820.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            2158..2610
                     /gene="gatA"
                     /locus_tag="ACHX5T_003836"
     CDS             2158..2610
                     /gene="gatA"
                     /locus_tag="ACHX5T_003836"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR8468821.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            2641..2925
                     /gene="gatB"
                     /locus_tag="ACHX5T_003837"
     CDS             2641..2925
                     /gene="gatB"
                     /locus_tag="ACHX5T_003837"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR8468822.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            2929..4284
                     /locus_tag="ACHX5T_003838"
     CDS             2929..4284
                     /locus_tag="ACHX5T_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR8468823.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         1051 a          968 c         1152 g         1148 t
ORIGIN      
        1 aaatgtacgt ggtatcgaca aagcagatgc tgaacaacgc acagcgcggc ggttatgcgg
       61 ttccggcatt caatattcac aatctcgaaa cgatgcaagt ggtggtggaa accgctgcca
      121 acctgcatgc gccggtcatc atcgccggaa cgcctggcac atttactcat gctggtacag
      181 aaaatctgtt ggcgctggtc agcgcgatgg cgaagcaata tcaccatcca ctggcaattc
      241 atctcgacca tcacacgaaa tttgacgata tcgctcagaa ggttcgttct ggcgtgcgct
      301 cagtcatgat tgacgcctcg catttgcctt ttgcgcaaaa tatttcacgg gtcaaagagg
      361 tggtggattt ttgccatcgc tttgatgtca gcgtcgaggc ggagctgggg caacttggcg
      421 gccaggaaga tgatgtgcaa gtcaatgaag tcgatgcgtt gtacaccaac cccgcacagg
      481 cgcgtgaatt tgccgaggca accgggattg attccctggc ggtcgccatc ggcacggctc
      541 acggtatgta tgccagcgca ccggtgcttg atttttctcg actggagaac attcgccagt
      601 gggtgaactt accgctggtg ctgcatggcg cgtcaggatt atcgactaag gatattcagc
      661 aaaccatcaa actggggata tgcaaaatca acgttgcaac ggagctgaaa aatgccttct
      721 tgcagtcgtt aaaaaattac ctgaccgagc accccgaagc gaccgatccc cgggattatt
      781 tgcagtcggc taaatccgca atgcgcgatg tggtgagcaa agtgattgcc gattgtggct
      841 gcgagggcag ggcataacgc acctgccatt taacaaggaa aaaacatgaa aacgttaatt
      901 gcccggcata aagctggtga acatatcggc atatgttcag tctgttctgc ccatccgttg
      961 gttatcgaag cggcgctggc atttgatcgc aacagcacgc gcaaagtgct gattgaagca
     1021 acgtcaaacc aggtcaatca atttggcggt tataccggaa tgacaccggc agactttcgc
     1081 gaatttgttt ttacgattgc cgataaagtt gggtttgcac gcgaacgcat tattctcggc
     1141 ggcgatcatc tgggaccaaa ctgctggcag caagaaaatg cggatgcggc gatggaaaaa
     1201 tccgtcgagc tggtaaaagc atatgttcgt gctggcttca gtaaaattca tcttgatgcg
     1261 tcaatgtcct gcgcggggga taccataccg ttagcaccag aaacggttgc ggaacgagct
     1321 gctgtgcttt gctttgctgc ggaaagtgtg gcgacagatt gccagcgtga gcaactgagc
     1381 tatgtcattg gcactgaagt tccggttccg ggcggtgagg ccagcgccat tcagtcagta
     1441 cacatcaccc atgttgaaga tgccgccaat actttacgta cgcatcaaaa ggcctttatt
     1501 gcccgtgggc tgacagaggc gttaacacgt gtgattgcca tcgtggtgca gccgggtgtg
     1561 gaatttgatc acagcaatat tatccattat cagccgcagg aagcgcaggc gctggcgcaa
     1621 tggatagaaa acacccgaat ggtttatgaa gcacattcta ccgattacca gacccggacg
     1681 gcttattggg aattagtccg cgatcacttt gcaatattga aagtcggtcc cgcattaacc
     1741 tttgctttac gcgaggcgat atttgcactg gcacaaattg agcaggaact tatcgccccc
     1801 gaaaatcgca gcggttgcct ggcggtaatt gaagaagtga tgctcgacga accgcaatac
     1861 tggaaaaaat attatcgcac gggttttaac gattcattac tggatattcg ttacagcctg
     1921 tcggatcgta ttcgttatta ctggccgcat agtcggatta aaaatagcgt tgaaacgatg
     1981 atggtgaatc tgcaaggcgt ggacatccca ctgggcatga ttagtcagta tcttcccaaa
     2041 caatttgaac gcattcagtc cggggaatta tcagcaatgc cgcatcagct tattatgaat
     2101 aaaatttatg atgttttgcg cgcctatcgc tacggctgtg cggaataagg acggtatatg
     2161 actaacctgt ttgttcgtag cggaatttct tttgtcgatc gtagcgaagt tttaacctat
     2221 atcggtaatg agatgctcgc gaaaggtgtg gttcatgata cctggccaca ggcattaatt
     2281 gccagagaag cagaattccc taccgggata atgcttgagc agcacgccat tgcaataccg
     2341 cattgtgagg cgattcatgc taagtcgtca gccatttatc tgttaaggcc aacaaataaa
     2401 gttcattttc agcaagcgga tgatgataac gacgtggcgg tatcgttggt tattgcgttg
     2461 attgtggaaa atccgcagca gcaattgaaa cttttacgct gtttatttgg caagttacaa
     2521 cagcccgata tcgtcgagac actaattact cttcctgaaa ctcagttaaa ggaatacttc
     2581 acaaagtatg ttttagattc agacgaataa atccctctgt aacaataaaa aggactattt
     2641 atgaaacgca agattattgt cgcttgcgga ggcgcggttg cgacctctac gatggcggcg
     2701 gaagaaatta aagagttgtg tcagagtcat aatattcctg ttgaattaat ccagtgtcgg
     2761 gttaatgaaa tagaaaccta tatggatggt gtgcatttga tatgtaccac tgccagggtg
     2821 gatcgtagtt ttggcgatat tccgttagtt cacggcatgc cttttgtttc tggtgtcggt
     2881 atcgaagcat tacaaaataa aattctgact atcttacagg gatgacctat gttttcagaa
     2941 gtcatgcgtt atattctcga cctcggccct acagtgatgc tgccggttgt catcattatt
     3001 ttttctaaaa tattaggcat gaaggcaggc gattgcttta aagcgggtct gcatatcggg
     3061 attggctttg ttggcattgg ccttgtgatt ggcttaatgc tggattccat tggtccggcg
     3121 gcgaaagcga tggcggaaaa tttcgacctg aatctgcatg tggtcgatgt cggctggccg
     3181 ggctcttcac caatgacctg ggcgtcgcaa attgcgctgg tggcgattcc gattgcgata
     3241 ttggttaacg tggcgatgtt actgacccgt atgacgcggg tggtaaatgt tgatatctgg
     3301 aatatctggc atatgacctt caccggcgcg ttgctgcacc tggcaaccgg ttcatggatg
     3361 atagggatgg cgggtgtggt aattcacgcg gcgtttgttt ataagctcgg cgactggttt
     3421 gcccgcgata cccgaaattt ctttgagctg gaaggtattg ctattccgca cggtacgtcg
     3481 gcgtatatgg ggccgattgc ggtgctggtc gatgctatca tcgagaaaat cccaggcgtt
     3541 aaccgaatta aatttagcgc cgacgatatt cagcgcaaat ttggcccttt tggcgaacct
     3601 gtcaccgtgg gttttgtgat ggggctgatt atcggcatcc tcgcgggtta cgatgtcaaa
     3661 ggtgtattgc agctggcggt aaaaacggcg gcagttatgc tgctgatgcc acggatgatt
     3721 aaacccatca tggatggttt aacgcccatc gctaagcagg cccgtagtcg tttacaggcg
     3781 aagttcggcg gtcaggagtt cctgattggc ctggatcccg cattactgct ggggcatacg
     3841 gcggtggtat cggcaagcct gatttttatt ccgctcacca ttttaattgc tgtttgtgtt
     3901 ccgggtaatc aggtactgcc gtttggcgat cttgccacta tcggcttctt cgtggcgatg
     3961 gcggtcgcgg tgcatcgtgg aaatctgttc cgcaccttaa tctcgggtgt catcattatg
     4021 agcatcaccc tgtggatcgc gacgcaaact attggtttgc acacccaacc ggcggctaat
     4081 gctggggcgt taaaagccgg gggtatggtg gcttcaatgg atcagggcgg ttctcccatt
     4141 acctggttac tgattcaggt tttctccccg caaaatattc ccggtttcat tattatcggc
     4201 gcaatttatc tgaccggtat tttcatgacc tggcgtagag cgcgtggttt tactaaacaa
     4261 gagaaagccg ttctcgcaga ataattttta cctgaggggg aggttatccc ccttaacaa
//
LOCUS       DAZSIO010000232         4274 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZSIO010000232 DAZSIO010000000
VERSION     DAZSIO010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.236"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            238..1170
                     /gene="rfaD"
                     /locus_tag="ACHX5T_003839"
     CDS             238..1170
                     /gene="rfaD"
                     /locus_tag="ACHX5T_003839"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR8468824.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1180..2226
                     /gene="rfaF"
                     /locus_tag="ACHX5T_003840"
     CDS             1180..2226
                     /gene="rfaF"
                     /locus_tag="ACHX5T_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR8468825.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2230..3222
                     /gene="rfaC"
                     /locus_tag="ACHX5T_003841"
     CDS             2230..3222
                     /gene="rfaC"
                     /locus_tag="ACHX5T_003841"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR8468826.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3219..>4274
                     /gene="rfaL"
                     /locus_tag="ACHX5T_003842"
     CDS             3219..>4274
                     /gene="rfaL"
                     /locus_tag="ACHX5T_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFR8468827.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNG"
BASE COUNT         1125 a          969 c         1047 g         1133 t
ORIGIN      
        1 attatccatt caaatgattc cgttatttat catacatcgg aatattgata ctaaagcact
       61 attcacatgc aaaaccaaca tccgccatga aggactagct aaaacccaaa ctagtttgtt
      121 gcaattagca tccttgcacc tctatgtaaa gggctgaagg gattcggatg tgatggtatg
      181 attacagaca ttcgtgtctg agattgtctc tgactccata attcgaaggt tacagttatg
      241 atcatcgtta ccggcggcgc gggctttatc ggcagcaaca tcgttaaggc cctgaatgat
      301 aaaggcatca ccgatattct ggtggtggac aacctgaaag acggcaccaa gtttgtgaac
      361 ctggtggatc tggatatcgc ggactatatg gataaggaag acttcctgat ccagattatg
      421 gctggcgaag agttcggtga tgtcgaagcg attttccacg aaggcgcatg ctcttccacc
      481 accgagtggg atggcaagta tatgatggat aacaactatc aatactccaa agagctgctg
      541 cactactgtc tggagcgcga aatcccgttc ctgtatgcct cttccgcagc cacctacggc
      601 ggacgcacct ccgactttat tgaatcccgc gagtacgaaa aaccgttgaa tgtctacggt
      661 tactcaaaat tcctgtttga tgaatatgtt cgtcaaatcc tgccagaagc gaactcgcag
      721 attgttggct tccgctattt caacgtttat ggaccgcgtg aaggccataa aggcagcatg
      781 gcgagcgtcg ctttccatct caacacccag cttaataacg gtgaatcacc gaagctgttc
      841 gaaggtagcg agaacttcaa acgtgacttc gtctatgtgg gcgacgtggc agatgtaaac
      901 ctgtggttcc tggaaaatgg cgtttccggt atcttcaacc tcggcaccgg tcgtgcagaa
      961 tccttccagg cagtagcaga tgctacgctg gcttatcaca agaaaggcca gatcgaatac
     1021 attccgttcc cggataagct gaaaggccgc taccaggcgt tcactcaggc agatctgaca
     1081 aatctgcgcg cggcgggtta cgacaaaccg ttcaaaaccg ttgctgaagg tgtaacggaa
     1141 tacatggcct ggctgaatcg cgacgcataa gagctctgca tgaaaatact ggtgatcggc
     1201 ccgtcttggg ttggcgacat gatgatgtcg caaagtctct atcgcacgct ccaggcgcgc
     1261 tatccccagg cgataatcga tgtgatggca ccggcatggt gccgtccatt attatcgcgg
     1321 atgccggaag ttaacgaagc tatccctatg cctctcggtc acggagcgct ggaaatcggc
     1381 gaacgccgca aactgggtca tagcctgcgt gaaaagcgct acgaccgcgc ctacgtctta
     1441 ccaaactcct tcaaatctgc attagtgcct ttcttcgcgg gtattcctca tcgcaccggc
     1501 tggcgcggcg agatgcgcta cggtttactc aacgatgtac gcgtgctcga taaagaagcc
     1561 tggccgctaa tggtggaacg ctatgtcgcg ctggcctatg acaaaggcat tatgcgtacc
     1621 gcacaagatc tgccgcagcc attgttatgg ccgcagttgc aggtgagcga aggtgaaaaa
     1681 tcatatacct gtaatcaatt ttcgctttca tcagaacgtc cgatgattgg cttttgcccg
     1741 ggtgcggagt ttggtccggc aaaacgctgg ccacactacc actatgcgga actggcaaag
     1801 cagctgattg atgaaggtta tcaggtggtt ctgtttggct ctgcgaaaga tcatgaagcg
     1861 ggcaatgaga ttcttgccgc tttgaatacc gagcagcagg catggtgtcg gaacctggcg
     1921 ggggaaacac agcttgatca agcggttatc ttgattgcag cctgtaaagc cattgtcact
     1981 aacgattctg gcctgatgca cgttgcggcg gcgctcaatc gtccgctggt tgccctgtat
     2041 ggtccaagta gcccggactt cacaccgccg ctatcccata aagcgcgcgt gatccgtctg
     2101 attaccggct atcacaaagt gcgtaaaggt gacgctgcgg agggttatca ccagagcttg
     2161 atcgacatta ctccccagcg cgtactggaa gaactcaacg cgctattgtt acaagaggaa
     2221 gcctgacgga tgcgggtttt gatcgttaaa acatcgtcga tgggcgatgt tctccatacg
     2281 ttgcccgcac tcactgatgc ccagcaggca atcccaggga ttaagtttga ctgggtggtg
     2341 gaagaagggt tcgcacagat tccttcctgg cacgctgccg ttgagcgagt tattcctgtg
     2401 gcaatacgtc gctggcgtaa agcctggttc tcggccccca taaaagctga acgcaaagcg
     2461 tttcgtgaag cgctacaagc agagaactat gacgcagtta tcgacgctca ggggctggta
     2521 aaaagcgcgg cactggtgac acgtctggcg catggcgtaa agcatggatt ggactggcaa
     2581 accgctcgcg aacctttagc cagcctgttt tacaattgta agcatcatat tgcaaaacag
     2641 cagcacgccg tagaacgcac ccgcgaactg tttgccaaaa gtttgggcta tagcaaaccg
     2701 caaacccagg gcgattatgc tatcgcacag cattttctga cgaacctgcc tacagatgct
     2761 ggcgaatatg ccgtatttct tcatgcgacg acccgtgatg ataaacactg gccggaagaa
     2821 cactggcgag aattgattgg tttactggct gattcaggaa tacggattaa acttccgtgg
     2881 ggcgcgccgc atgaggaaga acgggcgaaa cgactggcgg aaggatttgc ttatgttgaa
     2941 gtattgccga agatgagtct ggaaggcgtt gcccgcgtgc tggccggggc taaatttgta
     3001 gtgtcggtgg atacggggtt aagccattta acggcggcac tggatagacc caatatcacg
     3061 gtttatggac caaccgatcc gggattaatt ggtgggtatg ggaagaatca agtagagtgt
     3121 cgttctacaa gtatgtctct tgcagatttg ccagctcaaa ccgtttttca aaatttaaat
     3181 cttgaaataa taaccaataa gttgacatcg gagataagat gacctcaaca ttatttttct
     3241 ctctcgagaa aaaaaactgg atagcgtact ggaacagagc tctcgtattc ttattcatta
     3301 ccacctattt tttgggtggg attacaaggt ataaacatct tattgttatt cttatgacaa
     3361 taacgacaat cgtctatctc tgcaaacggc caaaacacta tctctcacta tttaaaacat
     3421 ttctttttgg tagtgttgcc atattaacta ttgctgcatt gctgtcactt cttcaatccc
     3481 ctgatgcagg tgctagcatg aaggaagttt tcaaagctat tattgagaat actttactat
     3541 gcacaatagc aataccggtc atattgagag acgagaaaag agaagatgtc gaaaaaatcg
     3601 ttttcttctc atttattagt gcgttgggct tacgctgttt ttctgaattg attacctatt
     3661 ataaggacta tcaacaaggg ataatgccat tcgcagatta tagacaccgt agcatttctg
     3721 actcgatggt ctttttattc cctgcattgt taaatctctg gcttatcaaa tcagcaaaat
     3781 accgcatttc ttttgtggtt ctaagcgtta tttttatttt tctgatatta ggaactttat
     3841 ccagaggggc ctggctttcc gtgttagtca ttggattaat atggattctg atgtttaaac
     3901 aatggaagtt actattagta ggagtaatgg tttccatcat tgcattgtcg gttattttca
     3961 cacataagga gatgaccgca aagctaacgt ataaacttca acaaactaat agttcttatc
     4021 gctatgcaaa tggtactcaa ggcagcgcac tcgatctaat attagaaaat cctgttattg
     4081 gttatggtta cggtaacgtt gcatataaag atgtctataa taaacgtgtc attgattatc
     4141 cagaatggac ctttagacaa tcaatagggc cacataattt tgcgctattc atctggtttg
     4201 gcactggttt attagggctg gtaagtctta tgatgctata ctgtgcaata ttgaaagagt
     4261 gtataaaaaa tggc
//
LOCUS       DAZSIO010000233         4221 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZSIO010000233 DAZSIO010000000
VERSION     DAZSIO010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.237"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(87..2063)
                     /gene="speA"
                     /locus_tag="ACHX5T_003843"
     CDS             complement(87..2063)
                     /gene="speA"
                     /locus_tag="ACHX5T_003843"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR8468828.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="ACHX5T_003844"
     CDS             complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="ACHX5T_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR8468829.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            complement(2297..2440)
                     /gene="yqgG"
                     /locus_tag="ACHX5T_003845"
     CDS             complement(2297..2440)
                     /gene="yqgG"
                     /locus_tag="ACHX5T_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR8468830.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2551..2802)
                     /gene="yqgD"
                     /locus_tag="ACHX5T_003846"
     CDS             complement(2551..2802)
                     /gene="yqgD"
                     /locus_tag="ACHX5T_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR8468831.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2858..4012
                     /gene="metK"
                     /locus_tag="ACHX5T_003847"
     CDS             2858..4012
                     /gene="metK"
                     /locus_tag="ACHX5T_003847"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR8468832.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         1078 a         1138 c         1021 g          984 t
ORIGIN      
        1 agcagctaga ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
       61 tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa
      121 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
      181 acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg
      241 tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg
      301 aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga
      361 tatgcaccga ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt
      421 ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca
      481 caggtaatat ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt
      541 agaaccggga acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa
      601 ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca
      661 cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct
      721 gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc
      781 agatccatct ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc
      841 atttcctgcc aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg
      901 gtcggcaccg tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg
      961 tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct
     1021 tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt
     1081 acggagcagt cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg
     1141 tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca
     1201 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt
     1261 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     1321 gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc
     1381 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     1441 tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc
     1501 ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg
     1561 acgctacgag tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct
     1621 tccagaccca gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta
     1681 actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt
     1741 ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt
     1801 gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     1861 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     1921 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     1981 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     2041 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     2101 taacgactgt cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     2161 gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     2221 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     2281 cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat
     2341 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac
     2401 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     2461 gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc
     2521 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     2581 aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc
     2641 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa
     2701 ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     2761 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     2821 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     2881 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     2941 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     3001 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     3061 cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa
     3121 ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc aaggcgttga
     3181 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     3241 taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg
     3301 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     3361 ggtgactttc cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac
     3421 tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     3481 caagccaatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     3541 cggtcgtttc gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat
     3601 cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc
     3661 atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc
     3721 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     3781 gacctccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct
     3841 gctggtacgt gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct
     3901 gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg
     3961 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt
     4021 cacctgcgtt caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     4081 aggcgttcac gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat
     4141 cacgtttcac ggcaaattac catcacaact gaaaccgatt acaccaacca caccagcaaa
     4201 atatttgtaa tattttcata t
//
LOCUS       DAZSIO010000234         4209 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZSIO010000234 DAZSIO010000000
VERSION     DAZSIO010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.238"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..209)
                     /locus_tag="ACHX5T_003848"
     CDS             complement(<1..209)
                     /locus_tag="ACHX5T_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR8468833.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHX5T_003849"
     tRNA            complement(324..399)
                     /locus_tag="ACHX5T_003849"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHX5T_003850"
     tRNA            complement(406..480)
                     /locus_tag="ACHX5T_003850"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHX5T_003851"
     tRNA            complement(597..681)
                     /locus_tag="ACHX5T_003851"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHX5T_003852"
     tRNA            complement(690..765)
                     /locus_tag="ACHX5T_003852"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHX5T_003853"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHX5T_003853"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR8468834.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHX5T_003854"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHX5T_003854"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR8468835.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHX5T_003855"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHX5T_003855"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR8468836.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1046 c          937 g         1137 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAZSIO010000235         4198 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZSIO010000235 DAZSIO010000000
VERSION     DAZSIO010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.239"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            84..752
                     /gene="ykgH"
                     /locus_tag="ACHX5T_003856"
     CDS             84..752
                     /gene="ykgH"
                     /locus_tag="ACHX5T_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFR8468837.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(995..1690)
                     /locus_tag="ACHX5T_003857"
     CDS             complement(995..1690)
                     /locus_tag="ACHX5T_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR8468838.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1683..3110)
                     /locus_tag="ACHX5T_003858"
     CDS             complement(1683..3110)
                     /locus_tag="ACHX5T_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR8468839.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3121..3840)
                     /locus_tag="ACHX5T_003859"
     CDS             complement(3121..3840)
                     /locus_tag="ACHX5T_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR8468840.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1119 a         1085 c          869 g         1125 t
ORIGIN      
        1 ttgctgatat ttaacccgaa cattcctgat acagactatt aatacaacat tatctgcgac
       61 agattttatt ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg
      121 agattttatt tcatctgaag aaaaagattc gtgttatcct ttttattatg gctatctgta
      181 tggcaatggt gctgttgttt ctgtatatca ataaagacaa tacaaaagtg atttacagcc
      241 ttaaaataaa ccagacaacg ccaggtatac ttgttagctg tgatagcaat aataattttg
      301 cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt tttcagacca
      361 gcccagatat caagaacaga gaaataaggc tggaatggtc aggagataag agagatttac
      421 caactgctga agaggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag
      481 aataccataa tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg
      541 agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg
      601 gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg
      661 ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg
      721 tagatgaata ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg
      781 tataaagctg aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc
      841 tttttcactg taaatatcct ggcattacta atgcctggat atttacagaa gagcagcata
      901 accatgacgg aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc
      961 ttcttcaggt ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca
     1021 ccgctttcac cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgagc
     1081 tgggaccact gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat
     1141 gcaatttttc tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa
     1201 gagaagattc cgggagcagg ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca
     1261 cgcctcccga ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg
     1321 cctgcgagat attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct
     1381 gcaaacgttc actaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact
     1441 gatctcccag ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca
     1501 gctcacagcg cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct
     1561 gttggttaag ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt
     1621 gcggttcaag tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc
     1681 gattatccat tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct
     1741 ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt
     1801 ggtgttttgc cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact
     1861 ttccacaatc ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct
     1921 tttgcggtga tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc
     1981 ggaatacgca ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa
     2041 tctttaaaat ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga
     2101 taaatagagc catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa
     2161 gccccgcagc gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc
     2221 ccgttatcga caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc
     2281 ggccctgtca gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc
     2341 atggtgatca atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact
     2401 gcaatatgcg ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg
     2461 cataccgaac cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg
     2521 aggaaatctt cacggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc
     2581 ccctcatagc ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt
     2641 gccgggacca caacatgaga tggcggatct tgatccagct ggagaatata ttcacccaga
     2701 tcggtttcaa tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg
     2761 gtcaccatcg atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa
     2821 atgtagcggg tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc
     2881 actttttctg agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc
     2941 tgggcggccc gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga
     3001 tttgccccaa tacgctgctg cgcgcttgcc accgctttgc gcatgatcgg atcttcaatt
     3061 tgctgacgga tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc
     3121 tcagcggctc atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg
     3181 taatcgccca ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg
     3241 gacttccatc aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt
     3301 gaccgagaac gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc
     3361 cagtccacgc acatttttca gcagcgtaag tggctcgtcc ttcactccca gcttacgggc
     3421 caggctacaa gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac
     3481 ccctaattta ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc
     3541 acgtgatgcc cattcaggtt catccgccag atacgtcggg taacttttta tggcataggt
     3601 gcaagagcca gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt
     3661 tttcatccct ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca
     3721 tccctgtttc tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc
     3781 tcgtgccatt cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac
     3841 aactaatact ccattacttc atgcccattt atgcccgatg tttccccccc accgggggat
     3901 caatcatcaa taaatatggc gttctttttt aattggctag caacatacca cccctcattt
     3961 gtatgacaaa tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt
     4021 cggcatcgct cacattttat ggggaaatcg acgatcattt ttggcataag aaatccattt
     4081 aaaaatggct acatccctta tacctattca gagctactta ttcaggggtt attgagatat
     4141 acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagtaa tagtcgga
//
LOCUS       DAZSIO010000236         4188 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZSIO010000236 DAZSIO010000000
VERSION     DAZSIO010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.240"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..192
                     /locus_tag="ACHX5T_003860"
     CDS             <1..192
                     /locus_tag="ACHX5T_003860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468841.1"
                     /translation="SIQSNRDWLSMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGIT
                     NIISIILQQAQDYDVAKIT"
     gene            complement(350..997)
                     /gene="ychE"
                     /locus_tag="ACHX5T_003861"
     CDS             complement(350..997)
                     /gene="ychE"
                     /locus_tag="ACHX5T_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR8468842.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(1028..1081)
                     /gene="ychE"
                     /locus_tag="ACHX5T_003862"
     CDS             complement(1028..1081)
                     /gene="ychE"
                     /locus_tag="ACHX5T_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFR8468843.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1474..4149
                     /gene="adhE"
                     /locus_tag="ACHX5T_003863"
                     /gene_synonym="adhC"
     CDS             1474..4149
                     /gene="adhE"
                     /locus_tag="ACHX5T_003863"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR8468844.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1168 a         1022 c          962 g         1036 t
ORIGIN      
        1 tcaattcaat caaatagaga ttggctaagc atgtctatct tttctgatta cagttccagt
       61 agtgaaatgc acaataattt aacaattgat tattatttag cactttcctc taccaaaggc
      121 agtggaatta ctaatatcat ttcaattatt cttcagcaag cccaggatta tgatgtggcg
      181 aaaataacat gatggtgagg agaagcagtt aaggaaatcc ttataactaa ctgtaatttg
      241 agaagaatat ttttacctat tccttttgct tttgataact gcgcgggaac aaagtaaaaa
      301 taatgacgaa gtaaacggag catggcagct ccgtttcatt tgaaaggaat taattaagca
      361 ggccggggaa aatcccctta ataccagtaa cgataaattc aatccccaat gccatcagca
      421 atagccccat aatacgcgta atcacgttga tgccggtctg gcgtaaaacc cgtaccagcc
      481 acggtgccat gcggaacaat ccccagcaac ataaagcgaa caatgcaata gccacaaaga
      541 aaccaaacag atagctaatg ctgtgataac gcgtacccca gacgatggta gaactgatcg
      601 cccctggccc cgccatcaac ggcaacgcca atggcaccac accaatactt tcacgtaccg
      661 cggtttctga tttttcttgc ttgttctgtt tatcctcgcc aagcttgccg ctgatcatcg
      721 acatcgctat cgtcaccacc aggatgcccc cggcgatacg gaacgaatca attgatatac
      781 caaaaagttg tagaatcgtg tcgccgagaa aaagcgagat ccacaagata atggccacag
      841 acaggttggc tgtaaggtta gttttgtttc gcgctgccgc tgtctgataa ctggtcatgc
      901 tgataaagac gggaataatc cctaccgggt tgaccagcgc aaataacccg atgaaaaatt
      961 tgaagtaaac gggaaaatca aaaagggtct gaatcacggt tcgctccgaa gcaaaagccg
     1021 gataatgtta gccataaata aggttgaaaa gacgcgctga caatacgcct tttgacagca
     1081 tttttcacct cctaactact taaaattgct atcattcgtt attgttatct agttgtgcaa
     1141 aacatgctaa tgtagccacc aaatcatact acaatttatt aactgttagc tataatggcg
     1201 aaaagcgatg ctgaaaggtg tcagctttgc aaaaatttga tttggatcac gtaatcagta
     1261 cccagaagtg agtaatcttg cttacgccac ctggaagtga cgcattagag ataataactc
     1321 taatgtttaa actcttttag taaatcacag tgagtgtgag cgcgagtaag cttttgattt
     1381 tcatcggtta agcaaatcat caccgcactg actatactct cgtattcgag cagatgattt
     1441 actaaaaaag tttaacatta tcaggagagc attatggctg ttactaatgt cgctgaactt
     1501 aacgcactcg tagagcgtgt aaaaaaagcc cagcgtgaat atgccagttt cactcaagag
     1561 caagtagaca aaatcttccg cgccgccgct ctggctgctg cagatgctcg aatcccactc
     1621 gcgaaaatgg ccgttgccga atccggcatg ggtatcgtcg aagataaagt gatcaaaaac
     1681 cactttgctt ctgaatatat ctacaacgcc tataaagatg aaaaaacctg tggtgttctg
     1741 tctgaagacg acacttttgg taccatcact atcgctgaac caatcggtat tatttgcggt
     1801 atcgttccga ccactaaccc gacttcaact gctatcttca aatcgctgat cagcctgaag
     1861 acccgtaacg ccattatctt ctccccgcac ccgcgtgcaa aagatgcaac caacaaagcg
     1921 gctgatatcg ttctgcaggc tgctatcgct gccggtgctc cgaaagatct gatcggctgg
     1981 atcgatcaac cttctgttga actgtctaac gcactgatgc accacccaga catcaacctg
     2041 atcctcgcga ctggtggtcc gggcatggtt aaagccgcat acagctccgg taaaccagct
     2101 atcggtgtag gcgcgggtaa cactccagtt gttattgatg aaactgctga tatcaaacgt
     2161 gcagttgcat ctgtactgat gtccaaaacc ttcgacaacg gcgtaatctg tgcttctgaa
     2221 cagtctgttg ttgttgttga ctctgtttat gacgcagtac gtgaacgttt cgcaacccac
     2281 ggcggctatc tgttgcaggg taaagagctg aaagctgttc aggatgttat cctgaaaaat
     2341 ggtgcgctga acgcggctat cgttggtcag ccagcctata aaattgctga actggcaggc
     2401 ttctctgtac cagaaaacac caagattctg atcggtgaag tgaccgttgt tgatgaaagc
     2461 gaaccgttcg cacatgaaaa actgtccccg actctggcaa tgtaccgcgc taaagatttc
     2521 gaagacgcgg tagaaaaagc agagaaactg gttgctatgg gcggtatcgg tcatacctct
     2581 tgcctgtaca ctgaccagga taaccaaccg gctcgcgttt cttacttcgg tcagaaaatg
     2641 aaaacagctc gtatcctgat taacaccccg gcttctcagg gtggtatcgg tgacctgtat
     2701 aacttcaaac tcgcaccttc cctgactctg ggttgtggtt cctggggtgg taactccatc
     2761 tctgaaaacg ttggtccgaa acacctgatc aacaagaaaa ccgttgctaa gcgagctgaa
     2821 aacatgttgt ggcacaaact tccgaaatct atctacttcc gccgtggctc cctgccaatc
     2881 gcgctggatg aagtgattac tgatggccac aaacgtgcgc tcatcgtgac tgaccgcttc
     2941 ctgttcaaca atggttatgc tgatcagatc acttccgtac tgaaagcagc aggcgttgaa
     3001 actgaagtct tcttcgaagt agaagcggac ccgaccctga gcatcgttcg taaaggtgca
     3061 gaactggcaa actccttcaa accagacgtg attatcgcgc tgggtggtgg ttccccgatg
     3121 gacgccgcga agatcatgtg ggttatgtac gaacatccgg aaactcactt cgaagagctg
     3181 gcgctgcgct ttatggatat ccgtaaacgt atctacaagt tcccgaaaat gggcgtgaaa
     3241 gcgaaaatga tcgctgtcac caccacttct ggtacaggtt ctgaagtcac tccgtttgcg
     3301 gttgtaactg acgacgctac tggtcagaaa tacccgctgg cggactatgc gctgaccccg
     3361 gatatggcga ttgtcgacgc caacctggtt atggacatgc cgaagtccct gtgtgctttc
     3421 ggtggtctgg acgcagtaac tcacgccatg gaagcttatg tttctgtact ggcatctgag
     3481 ttctctgatg gtcaggctct gcaggcactg aaactgctga aagaatatct gccagcgtcc
     3541 taccacgaag ggtctaaaaa tccggtagcg cgtgaacgtg ttcacagtgc agcaactatc
     3601 gcgggtatcg catttgcgaa cgccttcctg ggtgtatgtc actcaatggc gcacaaactg
     3661 ggttcccagt tccatattcc gcacggtctg gcaaacgccc tgctgatttg taacgttatt
     3721 cgctacaatg cgaacgacaa cccgaccaag cagactgcat tcagccagta tgaccgtccg
     3781 caggctcgcc gtcgttatgc tgaaattgcc gaccacctgg gtctgagcgc accgggcgac
     3841 cgtactgctg ctaagatcga gaaactgctg gcatggctgg aaacgctgaa agctgaactg
     3901 ggtattccga aatctatccg tgaagctggc gttcaggaag cagacttcct ggcgaacgtg
     3961 gataaactgt ctgaagatgc attcgatgac cagtgcaccg gcgctaaccc gcgttacccg
     4021 ctgatctccg agctgaaaca gattctgctg gatacctact acggtcgtga ttatgtagaa
     4081 gatgaaactg cagcgaaaaa agaagccgct ccggctaaag ctgagaaaaa agcgaaaaaa
     4141 tccgcttaat cagtagcgct gtctggcaat ataaacggcc ccttctgg
//
LOCUS       DAZSIO010000237         4182 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZSIO010000237 DAZSIO010000000
VERSION     DAZSIO010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.241"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..186)
                     /locus_tag="ACHX5T_003864"
     CDS             complement(<1..186)
                     /locus_tag="ACHX5T_003864"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR8468845.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
     gene            complement(327..2360)
                     /gene="metG"
                     /locus_tag="ACHX5T_003865"
     CDS             complement(327..2360)
                     /gene="metG"
                     /locus_tag="ACHX5T_003865"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR8468846.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2492..3601
                     /gene="apbC"
                     /locus_tag="ACHX5T_003866"
     CDS             2492..3601
                     /gene="apbC"
                     /locus_tag="ACHX5T_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR8468847.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3863..4144
                     /locus_tag="ACHX5T_003867"
     CDS             3863..4144
                     /locus_tag="ACHX5T_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR8468848.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1016 a         1043 c         1062 g         1061 t
ORIGIN      
        1 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
       61 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      121 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      181 tctcattctg catccctata attttatcca gttgaatgca gatgctacca gtatttatac
      241 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      301 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      361 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      421 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      481 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      541 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      601 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      661 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      721 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      781 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      841 aggaaggctt ctgcacgctc ggtcagtttc ggcagtacag gcttcaggta agtcatcagt
      901 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      961 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1021 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1081 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1141 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1201 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1261 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1321 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1381 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1441 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1501 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1561 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1621 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1681 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1741 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1801 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1861 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1921 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1981 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2041 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2101 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2161 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2221 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2281 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2341 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2401 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2461 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2521 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2581 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2641 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2701 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2761 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2821 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2881 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2941 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3001 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3061 caatgcgatg gtgtggcgtg gaccgatggc gggcaaggcg ttaatgcaga tgttgcagga
     3121 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3181 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3241 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3301 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3361 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3421 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3481 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3541 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3601 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3661 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3721 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3781 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3841 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3901 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3961 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4021 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4081 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4141 ttaatttggc aacctgattt taattgcatt tagaaagacg aa
//
LOCUS       DAZSIO010000238         4129 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZSIO010000238 DAZSIO010000000
VERSION     DAZSIO010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.242"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(42..299)
                     /locus_tag="ACHX5T_003868"
     CDS             complement(42..299)
                     /locus_tag="ACHX5T_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468849.1"
                     /translation="MKNYPTYGFYYPIANFIALQINGSKTGFAASITGFTQTISAGTI
                     SFLSVTVSDNGVGPGTTLGLGSLITSLLSIAVIAIAGNNKK"
     gene            755..1906
                     /locus_tag="ACHX5T_003869"
                     /pseudo
     CDS             755..1906
                     /locus_tag="ACHX5T_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc16 family transposase"
     gene            complement(1936..2364)
                     /locus_tag="ACHX5T_003870"
                     /pseudo
     CDS             complement(1936..2364)
                     /locus_tag="ACHX5T_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(2879..3922)
                     /locus_tag="ACHX5T_003871"
     CDS             complement(2879..3922)
                     /locus_tag="ACHX5T_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR8468850.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
BASE COUNT         1152 a          902 c          957 g         1118 t
ORIGIN      
        1 ccggggcggt tcaagtagat acatacgcag tcagagtaca cttacttttt attatttcct
       61 gcaatggcga ttacggctat tgacagtaat gaagttatta gtgaaccaag acctaaagta
      121 gttccgggac caacaccgtt gtcgcttacg gttactgaca ggaaactgat tgtaccagcg
      181 cttattgttt gagtaaaacc tgtaatagat gcggcaaatc ctgtttttga accatttatt
      241 tgtaatgcga taaaattggc tattgggtaa taaaaaccat aggttggata atttttcaat
      301 ctttgataat tgatttccga ttatgaaaaa taaaactgag aaggagaaaa taattgagta
      361 actaaaagta ctcaggccat aagtatgcat gaaaattgat ggtgatattg atatgtagca
      421 aaatagagaa ctgagtatta atgagacaag tatagtgccg cagataaact ctttggtcac
      481 aaaggatgca ctgttttgac atattacttt ctcatttgca tgagttatct ttggtgataa
      541 taacattatt ataatactga acagcatcat aacaagaaat ataacattcc agttataata
      601 ttcaataatc aatgcgcgag gtgctactaa tggtattact gcgcttacag atgatatatc
      661 agaaagcgcc cgttttagtt ttaatggagg gtgatcttac ccaccaatag tggacacagg
      721 actaagtgag taaactctca accagaggtg actcatgaca aaaccagtat caatcagcaa
      781 gaagccccgt aaacaacata cgcctgaatt tcgtaacgaa gccctgaaac tcgctgaacg
      841 catcggtgtg gccgccgcag cccgtgaact cagcctgtat gaatctcagc tttatgcctg
      901 gcgcagtaaa cagcagcaac aaatgagttc gtcagagcgc gaaagcgaac tggccgctga
      961 aaatgtccgc cttaaacgac aactggcgga gcaggctgag gaactgtcca tcctccaaaa
     1021 ggccgcgaca tacttcgcga agcgcctgaa atgaagtatg tctttatcga aaatcatcgg
     1081 gcagagttca gcatcaaagc gatgtgtcgt gtacttcggg ttgcccgcag cggctggtat
     1141 gtctggctca ggcgtcgtca ccagatgagc ctgcgccaac agtttcggct cacctgcgat
     1201 gccgctgtcc ataaggcatt ctttgaggca aagcagcgat acggtgctcc ccgccttgct
     1261 gacgaactgc cggagttcaa tattaaaacc attgccgcca gcctgcgtcg tcaggggctg
     1321 cgggcgaaag ccagccggaa gttcagcccg gtcagctacc gtgcacatgg cctgcccgta
     1381 ttggagaatc tgctggagca ggacttcagc gccagtggcc cgaaaccaga agtgggcggg
     1441 tgacatcacg tacttgcgta ccgatgaggg ctggttgtat ctcgcagtag tcatcgacct
     1501 gtggtcacgc gccgtcattg gctggtcgat gtcaccgcga atgacagcac aactggcctg
     1561 tgatgcactg caaatggcgt tgtggcggag aagacgcccg gaaagcgtca ttgttcatac
     1621 ggaccgtggt ggtcaatact gttcagggga ttatcaggca atgctgaagc gacacaacct
     1681 gcgtggcagt atgagtgcga aaggctactg ttatgacaat gcctgtgtgg aaagcttctt
     1741 tcattcgctg aaggtggaat gtatccacgg ggaacgcttt agcagccggg aaataatgcg
     1801 ggcaacggtg tttaattata tcgagtgtga ttacaatcgc tggcgtcgtc acagtgcctg
     1861 tggcggtctc agcccggaac aatttgaaaa ccataatctc gcttagggcc gtgtccacat
     1921 tacgtgggta ggatcagtat gggtcgtctt gtatttgtca tactgcccaa tctcaccaat
     1981 cgaaaatgct ccttttggca aaggttcaag caggtctctg ggaatggagg tcgctggcgt
     2041 cggatataaa aaaccctcac ggcttagcaa ggctggcgtc actatcggtg taacaatatc
     2101 aggcgtgcct gcggaacggt ccttaccatg ctgcagtaac cggaaataaa cttttgggcg
     2161 taagatgaca ttgacagttt cgacatcgtc tttttctccc ttaaaaaatt tattgacaat
     2221 cgcttcgtca agacgtcccg ccgctatccc atgccagtga gtgaaatttt gggcgtcttt
     2281 ccgttcaaaa cagccctttc ctgactcagc atctgcaagc gagttgcgcc agtatgaggc
     2341 aaaccttaaa gcattttcat ccattaccag tcctgtgact caatgaaatc cattgattta
     2401 tcgaatataa aaccaccgta tggacaacta ttgtccatat taaaaaagtt ggatgcaatg
     2461 ttttgtttat aattcgatat caaatgtact ggttgtacga ggttcagaac acaagcacat
     2521 tcaacagtac gtcaggacta ttgatttcat aacagcgaat acaatttttt actgtttata
     2581 atggctgcct gtgttggacg gggataccag ttcaaccgaa aacgccagag gaggggatta
     2641 cccgctggca gggtaaatct gtggtttttg atgccagcta taacgtgttt gacgataaag
     2701 tattacgaaa gaactttaat gagttcatgg agtaattcgt taaagtaatt tacaacgcta
     2761 tccttttgca aaggaaactt tcagaaaata accccagggt aatcacctgg ggaaggaaca
     2821 cagtgttatt attggataat aaattattac ttgttttaaa gttacactaa tgcaatatct
     2881 actgatactg gatgcttgaa tgtttcatcc ctgctgcgga aaagggcaat atccagggcc
     2941 agatatattc accgttcata tttctgcatt tctgtacaat ttcctcagaa agtgtcttgt
     3001 caattctttt ccacaggtgc tcctgctgtc ttaaaatact ttcgcatatt tcgaaatcat
     3061 cttcctgaat cagcatttcg gcgcatttaa tgatttctga tcgttcatgc agcacggagg
     3121 aatggttata gagccagttt acaaacagca atgcttccgg atattgtccc cataatggtg
     3181 agtatgttgc atagtaatct tctgccgtat ccagagagcc ggagaagtca tgccacacag
     3241 caagtggaaa tggatgcaga tttccgcaca gcatttctgc gatgtaagtg tttgtggcaa
     3301 agccatggcg aaaagctgtg gcggccttaa catgttcccc gttcagaaaa tgcaggagac
     3361 cgagttcata ccagtaaggt gaaaattcat cagcatgctc tttcagtaca ctgaaggctc
     3421 gctcatgatc gccggtgcgt aacagctctg aacccaacaa ccagcgagcc ccctgattat
     3481 cttcaggatt gtatgccagt attttatcag tgagcataac cgcatcctga tggcgctgta
     3541 agtgcacatt tgcgagaatt gctgcataaa gcgcgcgcag ataaggccgg ttttcgggat
     3601 gcatccagat tatttcacca cagaaacttt ccgggatgat acgattacct gccgccagtc
     3661 ctttaagagc ggcgttcagt gccttacgcg gggcattctg ttccagaaat gcatatgcca
     3721 gatgagcgtg tatatcaata aagtccggct cccgttgagc aaggcgtcgg agtttgttga
     3781 tgtaactcgt ctcgctgatt tcatcagcgt cgtatgcatc aagcacttct tcaaactccc
     3841 ccagcaatgg gccaaaccag gtatccggaa atacgaaata acccttgtta tcgtgcactt
     3901 caaagcataa tctatcgttc attttctgag cctgtaaaac atgtggtgat atagtctgag
     3961 agcataaaag ttgagattct gtgtatcaag tcctgtcttg gcaccgaatg aatattgctt
     4021 acgaagcccc ttcatgcttt ccttgagaac aataatttta tctttatgtg aatctcattt
     4081 tttataatga tgtcagatga ttgaaatgct aaattatatc tgaatcaac
//
LOCUS       DAZSIO010000239         4115 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZSIO010000239 DAZSIO010000000
VERSION     DAZSIO010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.243"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            89..931
                     /locus_tag="ACHX5T_003872"
     CDS             89..931
                     /locus_tag="ACHX5T_003872"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR8468851.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            934..2022
                     /gene="capU"
                     /locus_tag="ACHX5T_003873"
     CDS             934..2022
                     /gene="capU"
                     /locus_tag="ACHX5T_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFR8468852.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            2027..2977
                     /gene="virK"
                     /locus_tag="ACHX5T_003874"
     CDS             2027..2977
                     /gene="virK"
                     /locus_tag="ACHX5T_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR8468853.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            3042..3986
                     /gene="lpxM"
                     /locus_tag="ACHX5T_003875"
                     /gene_synonym="msbB"
     CDS             3042..3986
                     /gene="lpxM"
                     /locus_tag="ACHX5T_003875"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR8468854.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         1173 a          771 c          950 g         1221 t
ORIGIN      
        1 tgttattcat ccagtatctg gaaataaaat gcaccagctt tattgggttt tccttttgtc
       61 tctgcttgct aggtgccaga aactttaaat gttaaacgaa gggggcattt tgttcaaagc
      121 caatcatgtt ccggtattaa tgtatcatca tgtcagtcat tgccccggac tggttacact
      181 ttctcccgtg acattccgga agcaaataaa atggttggct gaaaacaact ggaaaacact
      241 gtcatcagat gaactggagt ttttttatcg gggcggaaaa ctcccccgga aaagcgttat
      301 gttgacattt gatgatggat accttgacaa ctggtttcag gtatatcctt tattaaaaga
      361 gtttaatctc aaagcacata tttttttaat taccggtttt attgggaatg gtccggttcg
      421 tcactctcct ggaaaggaat attctcatcg cgattgcgaa catcaaatag cgacagggaa
      481 tgctgataat gttatgcttc gctggtctga agtaaacgaa atgttgcaat ctggtctggt
      541 cgaatttcat gttcatacgc atactcatac acgctgggat aaaaagttca gttcacgaga
      601 agaacagtgt aaacatcttc ggcaggactt gttgagcggg agggaatatt tgaaagaaat
      661 gacaggaaaa tgcagtaaac atttgtgttg gccggaaggt tattataaca aggattatat
      721 tcaggtcgct gaagaacttg gattctatta tttatataca acagaaagaa gaatgaatgc
      781 tcctgctaaa ggaactacac ggataggaag aataagcacc aaagaacggg aaagttgcgc
      841 atggctgaaa agacgacttt tttattatac aaccccgttt ttttcatcgt tgctggcctt
      901 tcataagggg ccaagattac cggatgatta ataatgaata tactatttac ggaatcatca
      961 ccgaatatag gtggtcagga attacaggct gttgctcaaa tgaaggccct gaagaaaatg
     1021 gggcattcag ttctgcttgt ctgcagggaa aacagcaaaa ttgcttttga agccagtaaa
     1081 ttggggattg atatcacatt cgcgttattt cgaaacagtc ttcacatccc tactgcatgg
     1141 agattactcg gaatagttca tggttttcag cccaatgcaa tcgtttgtca cagtgggcat
     1201 gatagcaata ttgttggttt agtacggtta ttcactagga aacatccatt cagaattatc
     1261 aggcaaaaga catatttgac acgaaaaaca aaagtttttt caataaatca tttttgcgat
     1321 gaggtgattg ttcccggaac aagtatgaag acacatctgg agcaggaagg atgtcgaacc
     1381 cgggttactg ttgtgcctcc aggctttgat ttccagaaat tatacgttga ttcccgaaac
     1441 agtttgcctc caaatgttct ttcttggctg gcgtcccgaa ggggatgccc cgttattgct
     1501 caggtaggaa tgttgcgccc ggaaaaaggg cacgaattta tgttgaattt actgttccat
     1561 ttaaaaatga atggacgaca gttctgttgg ttgattgtgg ggtctggttc gcctgaactg
     1621 cgggagcatt tacagtatca gattgacagt atgggcatgc atgatgatgt ttttattgct
     1681 gacaatgttt ttcctgccgc ccccgtatat cgggttgcca gtctggtggt tctgccttca
     1741 gaaaacgaat cgtttggtat ggtactggca gaagcatcgg cattttctgt gcctgtactg
     1801 gccagtcaga ttggtggaat ccctgatgtt attcagaata accagaccgg gacattgtta
     1861 ccagcaggta ataagcacgc atggatgtgc gccctgaatg atttttttaa tgaccctggg
     1921 cgtttttatc agatggctcg ccaggcaaaa caggatatag aagagcggtt tgatattaat
     1981 aaaactgcgt taaaaatact cacattagcg aagcacaagt aacaatatgt tttctgtaag
     2041 taacttatca tttatcggtt tccttaaaag gattgttttt tcctcagatt cactcccggg
     2101 gaagtgggaa cacagaaaat tccggttcat gtacattttg cgatgtgcta taaatccggt
     2161 tgccagtatt cgatattatt acgaactgcg ttccttgcag tgcattgagg atattctggc
     2221 aatacaaccc acgttgccag caagaattca tcgaccttat ttgcataaag gggggcgagc
     2281 ctggtcccgg gggcaatata ttcttgagca ttatcgcttt gtgcaaaatt tgccagaaaa
     2341 atattctgaa tttttgtttc cacaaaaatc ggtatcatta gtccaattca tcgggaaaga
     2401 tggagaggat tttgatatcc agtgttctcc cagtggtttt gaccgggaag gagagttgat
     2461 gttgtcattg tttttcaata aaatagtgat tgcccggtta acattttcag tcattctgac
     2521 tcaaaatgga cacaccgctt ttattggcgg attacaggga gcccccaaaa ataccggacc
     2581 tgatattatt cggtgcgcaa cccgggcctg ttatgggctt ttcccaaaac ggattatttt
     2641 tgaagcattt tgtgcgttga tgaaagcttg taatgtctct gagtgtctgg cagtcagtga
     2701 gcacagtcat gttttccggc aattgagata ctggtatcag aaacgcaaaa cctttgttgc
     2761 tgtctacagt gatttctggg agtctgtagc agggaaaacc tgtggtgact ggtacaaatt
     2821 accaacacag gtggttcgaa agccgctaag caatatagct agtaaaaaac gctctgagta
     2881 tcgaaaacga tacgctttgc tggattatat ccatgaaact gctatccgct ctttggatgc
     2941 atatcctgta aactcagaac atcaggactt aaattaaggt ttgatgtatt ctctgaataa
     3001 aatattaatg atgattttgg taggggcatt cgcactaaat aatgaaaaaa tacaaatccg
     3061 agtttattcc tgaatttaag aaaaattatc tttcccctgt ttactggttt acatggttcg
     3121 ttttgggaat gattgcaggt atttcaatgt ttcccccttc attcagagat cctgtcttgg
     3181 ccaaaatagg gcgttgggtg ggcagattga gcagaaaagc tcgtcgcagg gcgacgatta
     3241 atttatcgct ttgtttcccg gaaaagagtg atacagaacg ggaaataatt gtcgacaata
     3301 tgtttgccac agcattgcaa tccatagtga tgatggcaga actggcaatt cgtggtccgg
     3361 aaaagttcca gaaacgagtg ttctggaaag gcctcgaaat tcttgaggaa attcggcaca
     3421 ataacagaaa tgtgattttt ctggttcccc atggctggag cgtggatatt cctgcaatgt
     3481 tgctggcagc tcagggggaa aaaatggccg ccatgtttca tcagcaacga aatccagtga
     3541 ttgattatgt ctggaattct gtacggcgta aattcggggg gcgcttacat tcccgggagg
     3601 atgggataaa accatttatt cagtcagtac gccagggata ctgggggtat taccttccag
     3661 atcaggatca tggtcctgaa tacagtgaat ttgctgattt ttttgcgacc tataaagcga
     3721 cattaccaat tattggacgt ctgatgaaca tcagtcaggc tatgattata ccgcttttcc
     3781 cggtttatga tgaaaaaaaa catttcctga ctattgaagt tcggccacca atggatgcat
     3841 gcattgccag cgcggacaat aaaatgattg cccgacaaat gaacaaaaca gtggaaattt
     3901 tggtggggtc acatccggaa cagtatatct gggttttaaa attgttaaaa acgcgcaaat
     3961 caaacgaagc ggacccgtac ccttgaaatt ataaagtaca ggtatttcca ctagttgttt
     4021 cttacaggtt accaatcgaa acacatcccc cttccgtgtg ttgcccgttt ggcacggagg
     4081 actgtgtaga tgcagttcgt taaatttaat ttatg
//
LOCUS       DAZSIO010000240         4114 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZSIO010000240 DAZSIO010000000
VERSION     DAZSIO010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.244"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..158)
                     /locus_tag="ACHX5T_003876"
     CDS             complement(<1..158)
                     /locus_tag="ACHX5T_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFR8468855.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="ACHX5T_003877"
     CDS             complement(308..469)
                     /locus_tag="ACHX5T_003877"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468856.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(469..654)
                     /locus_tag="ACHX5T_003878"
                     /pseudo
     CDS             complement(469..654)
                     /locus_tag="ACHX5T_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
     gene            914..1249
                     /locus_tag="ACHX5T_003879"
     CDS             914..1249
                     /locus_tag="ACHX5T_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR8468857.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1638)
                     /locus_tag="ACHX5T_003880"
                     /pseudo
     CDS             complement(1530..1638)
                     /locus_tag="ACHX5T_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2161..2237)
                     /locus_tag="ACHX5T_003881"
     tRNA            complement(2161..2237)
                     /locus_tag="ACHX5T_003881"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="ACHX5T_003882"
     tRNA            complement(2339..2414)
                     /locus_tag="ACHX5T_003882"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2559..3380)
                     /locus_tag="ACHX5T_003883"
     CDS             complement(2559..3380)
                     /locus_tag="ACHX5T_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR8468858.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3395..3751)
                     /locus_tag="ACHX5T_003884"
     CDS             complement(3395..3751)
                     /locus_tag="ACHX5T_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR8468859.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3764..>4114)
                     /locus_tag="ACHX5T_003885"
     CDS             complement(3764..>4114)
                     /locus_tag="ACHX5T_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR8468860.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT         1155 a         1118 c          793 g         1048 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgt
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgcc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattcca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccatta acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaaa
     2461 cccgctcagc ggcgggttgt agaaactctt ctaacgccag gcataaaacg cccatcatta
     2521 tgacgaattt accacagatt ccggaaaaat caaccttgtt acctagttac cttttttaac
     2581 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ataaaatttc
     2641 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2701 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2761 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2821 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2881 accacctccc tgactttttt ccattcttcc cagtaaaacg gatacacacc tttcgtacac
     2941 tttgcccata ccggcggctt accatccgga tactggatct tgtttgtaaa aactatgctt
     3001 tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca
     3061 taatcctcaa aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga
     3121 agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct
     3181 gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaaaccc
     3241 atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgca
     3301 tcttttgacg ttgaggccga tgcatcggtc aggccagggg attttgggga gtagtatttc
     3361 ggtaaatctt ccagtttcat tttttgacct gctcttaatg cattatgggg taaatcttca
     3421 cccccagacg tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct
     3481 catcgtccat taacactccc gcatgcgtca gcgcatccag cggtgctttc aggatattgt
     3541 ccaggtcgcg acgacgctta tccggtggct ctgcaatcac ctttatcgcc agccttccgg
     3601 acaggcttaa tttcagccgc tgctggcgaa caataagcgc cacagcccgg cgataacgct
     3661 ttccctcctc cgagataaaa tatgtgctgc cacggcgtcg ccagtaagtg ttcaccgtcg
     3721 gcgggtaagg taaaaccaaa tctatgagca tcagtcacct cttttaccca agcacgccag
     3781 ttgcaaaggc gtgatcaaga aaacgaaaaa ttaaatcaac ctgagaacca tgcttttctt
     3841 cgaacgccag aggatccgca tgaagctcgt tgtgatgctc ccgacacagc ggtagcgtga
     3901 aaatatcgtg agatttggtc cccattccgc cctgaccatg accaatcagg tgatggggat
     3961 cgtcggctgg cttaccacaa cacgcacacg gctgtgtctt cacccagcgt ttgtatttct
     4021 cgttaaccca gcggcgacgt ttaggtcgtt tcatgaaaga ttccggagac tcaggatcaa
     4081 cggcaatgct gaccaccgtc ttttcctgtt gtgg
//
LOCUS       DAZSIO010000241         4090 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZSIO010000241 DAZSIO010000000
VERSION     DAZSIO010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.245"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACHX5T_003886"
     CDS             complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACHX5T_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFR8468861.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(334..2273)
                     /gene="flhA"
                     /locus_tag="ACHX5T_003887"
                     /pseudo
     CDS             complement(334..2273)
                     /gene="flhA"
                     /locus_tag="ACHX5T_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520726.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2326..3473)
                     /gene="flhB"
                     /locus_tag="ACHX5T_003888"
                     /pseudo
     CDS             complement(2326..3473)
                     /gene="flhB"
                     /locus_tag="ACHX5T_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
     gene            3709..>4090
                     /locus_tag="ACHX5T_003889"
     CDS             3709..>4090
                     /locus_tag="ACHX5T_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFR8468862.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1015 a         1190 c         1003 g          882 t
ORIGIN      
        1 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
       61 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
      121 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
      181 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
      241 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
      301 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
      361 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
      421 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
      481 cgcacccagc atctcctgac gggatagagc ttcctgagtt tgcgccagta aacgatccgc
      541 cagccctggc tccagtcctc ccccgccctg cagcgcctgc gcccgtccca acgccacgcg
      601 cacgacggcg cttaattcat gtggatcgct ttggatgggc gcatgttccg ccagcgtttc
      661 gagaatggtg cgcatatcgc gaatcggtac tttttcatcg aggagatttt gcagcacttt
      721 atgcagtgtg gtgagcgtga cgacgccagg aacgagatct tccgtcagct ttggcatctc
      781 ctgggcgacg cgatccaaca gctgttgcgc ctcctgacga ccaaacagct ctgcggcatg
      841 ctggctaatg aggtggttaa gatgcgttgc taccaccgtg ctggcctcaa ccactgtgta
      901 cccctgaatc tgcgcctgtt cttttagcgc actttcaatc cagatagcat tcaggccaaa
      961 tgccggatcg acggtcgcct caccaggtaa cgtcccggca gcggttccag ggttaatcgc
     1021 cagccagcgc cccggataag catcaccact gccaatctcc acgcctttca tcaaaatgcg
     1081 atagcgggca ggttgcagat ccatattgtc gcgaatgtgc accactggcg gcagaaatcc
     1141 catctcctgg gcaaatttct tgcggatact gcgtatacgg cccaacaact caccatcctg
     1201 ctggaaatcg accatcggga tcagtcgata acccacttcc attcccagag aatcttccag
     1261 ttgtacatcg ttccacgtcg cttcgacaac ggtattattc tctgccattt ttaccggttt
     1321 gggttcggca ggcgcttttt gttcgcgtcc gcgtatccac caggccagcc ccagcaatcc
     1381 ggcagtgaac agcaaaaata ccaggttcgg cattccaggc accaggccga gtaaaccgag
     1441 cacggcggcg cttaacaaca taacgcttgg gttactgaaa agctgattca ccatctgctc
     1501 gccaacatcc tgatcggtgc tgacacgcgt aacgatgccc cccgcggcgg tagaaatcac
     1561 cagcgccgga atttgtgcca ccagaccgtc gccaatggtc aatagcgtat aactttccgc
     1621 cgcgtgtccc atgctcatgc cagttgcagc acgccgacca gcaacccgcc gacaacgtta
     1681 atgaccatga tgaggatccc ggcaatggca tcgccgcgaa caaacttact tgccccgtcc
     1741 attgagccgt aaaaatcggc ttcctgagtc acttcggagc ggcgtttttt cgcctcatct
     1801 tcaccaatca atccagcgtt aaggtcggcg tcaatcgcca tctgcttacc cggcatacca
     1861 tcgagaacaa agcgcgcacc cacttctgct atacgcccgg cacctttggt aatgaccata
     1921 aagttgatga tcacgagaat gacaaacacc acgataccga tagcgaaatt gccaccaacg
     1981 aggaagtgac cgaacgcttc gaccaccttc ccagccgccg ccgcgccggt atgcccttcc
     2041 atcaaaatga tacgggttga agccacgtta agtgccagac gcaacaacat agtaaacaac
     2101 agaatggtcg gaaacgcagc aaactcaagc gtgcgctggg taaacatcgc caccagcaac
     2161 accatgatcg acaaggcaat attgaaggta aacaacaggt cgagtatgaa tgcgggcagt
     2221 ggcagcacca tcatcgacaa gatcaacagg atcaaaatcg gtccggcaag gatctgccat
     2281 tatgtcgatt tcaggtttgc gggcaggcgc agcatcgcgg ccagattact catgggtcgg
     2341 tttctcgtta ataaaatcca gggctgccgg caccggaaga tgagtaggtg tacagggcgc
     2401 tgtccaccag ccagacgcca gcgtttcagt tgccagaccc aggccagcac ttccgccacc
     2461 gcggcgtaca gttgacccgg gatttgttga ccaatctccg catgtcgata cagcgctcgc
     2521 gccagcggcg gcgcttcaag cgtcgggacg ttattttcag cgccaatttc acgaatgcgc
     2581 agcgcgacca gccctgcacc tttagcgacc actttcggtg cgctcatttt gttttcgtca
     2641 tactgcaacg ctaccgaata gtgggtcggg ttattgacaa tgacatccgc tttcggcaca
     2701 tcggccatca tccgacgccg tgcggcagct cgctgcatct gacagatccg tcctttaaca
     2761 tgggggtcgc cttcgctttg tttgaactca tcacgaatat cctgccgcga cattcgcagc
     2821 tttttcaggt ggctgaagat ttggaaaaag acgtcaaatc ccaccattgg aatgacacca
     2881 agcaccacca gcagtgcgca tagccctacc aaatccatcg cattacccat ggcggtaatc
     2941 ggtgactcgg ccattaagcg catcatctgc ggccagtgat gccagagaaa aaaccccgtc
     3001 acgctgccaa ccaggatggt tttcaaaatt gccttaagca actccgcgct agtctgtgcc
     3061 gagaatatcc gtttaatgcc cggtagcggg ttgagtttgg aaaacttcgg ctgcaaggat
     3121 ttgccgctaa ataccagccc ccccagcata accggagaaa tgagcgccac cagcaccacg
     3181 ccgctaatca gcggtagcag cgccagcatg gcttctctga tcagcagaat aatttgcccg
     3241 aggatcagat tcgggtcttt gataatactg tgatcaaaat gcagcccagc ggagagcatg
     3301 cccgacaatc gacgggccag cgacacaccg ccaaaccaga taacactaac gcccaccagc
     3361 aaaatcagca gtgaggtcag ttcacgggaa cgcgggattt gcccctcttc ccgcgctttt
     3421 tctagtcggt gaggtgtggg ggcttctgtt ttgtcgtcgc tctcgtcaga cacgtcgcca
     3481 atcctggaaa gagtattaag cgtaaatgat gccagagcgc aaagcgttca atggtttaag
     3541 taaggggcaa aacaggcggg atttagggcg tttatgcaaa aataggttca gttgcagaac
     3601 gttttgcgaa ccattttcca gttctgatat ttagccattt agcctataga atcgcactcc
     3661 gcctctctgt tagtttgcta ataattaaat tattacaaag gtgatcaaat ggaatggcaa
     3721 caattgctta actgcaaccg tcggaaagat aatctaaaaa aaaccaacat agcattttta
     3781 catccccttg ctagcggaag acaagagatc gagcgggatt acgaccgatt actttttgct
     3841 gcaccaactc gtcggcttgc ggataaaaca caagttttcc cgttagatca aaatgacagc
     3901 atacggacga ggctgactca ttctcacgaa gtagcaaact ttgctcgcgg gataggaatg
     3961 aggctcgctt tcgagatgaa ggagcacatc tttggcgaaa tacccacggg gattgtcgtt
     4021 gaacgcgacg ttcctgctct gttagcagcg atcggactgg cacacgatct cggtaatcca
     4081 ccatttgggc
//
LOCUS       DAZSIO010000242         4049 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZSIO010000242 DAZSIO010000000
VERSION     DAZSIO010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.246"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            162..3470
                     /gene="icsA"
                     /locus_tag="ACHX5T_003890"
                     /gene_synonym="virG"
     CDS             162..3470
                     /gene="icsA"
                     /locus_tag="ACHX5T_003890"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HFR8468863.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            3614..3932
                     /locus_tag="ACHX5T_003891"
                     /pseudo
     CDS             3614..3932
                     /locus_tag="ACHX5T_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842854.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT         1218 a          702 c          962 g         1167 t
ORIGIN      
        1 caagacacag gtaaatttct cccgttgcat tgatatataa cacagctctc atgttttggt
       61 tgaggctttg tttaatatgt ttttgcatat atcgtccctt tattccggat aaaacggaat
      121 cttttcaggg gtttatcaac cacttactga taatatagtg catgaatcaa attcacaaat
      181 ttttttgtaa tatgacccaa tgttcacagg ggggggccgg agaattacct acggtaaagg
      241 aaaaaacatg caaattgtct ttttctcctt ttgttgttgg tgcatccctg ttgctcgggg
      301 ggccaatagc ttttgctact cctctttcgg gtactcaaga acttcatttt tcagaggaca
      361 attatgaaaa attattaaca cctgttgatg gactttctcc cttgggagct ggtgaagatg
      421 gaatggatgc gtggtatata acttcttcca acccctctca tgcatctaga actaagctac
      481 ggattaactc tgatattatg attagcgcag gtcatggtgg tgctggtgat aataatgatg
      541 gtaatagttg tggcggtaat ggtggtgact ctattaccgg atctgacttg tctataatca
      601 atcaaggcat gattcttggt ggtagcggcg gtagcggtgc tgaccataac ggtgatggtg
      661 gtgaggctgt tacaggagac aatctgttta taataaatgg agaaattatt tcaggtggac
      721 atggtggcga tagttatagt gatagtgatg gggggaatgg aggtgatgcc gtcacaggag
      781 tcaatttacc cataatcaac aaagggacta tttccggtgg taatggaggt aacaattatg
      841 gtgagggtga tggcggtaat ggaggtgatg ccatcacagg aagcagcctc tctgtaatca
      901 ataagggcac gttcgctgga ggcaacggag gtgctgctta cggttatggt tatgatggct
      961 acggtggtaa tgctatcaca ggagataacc tgtctgtaat caacaatgga gctattttag
     1021 gcggtaatgg tggacattgg ggggatgcta taaatggtag caatatgacc attgctaata
     1081 gcggatatat aatttcaggt aaagaagatg atggaacaca aaatgtagca ggtaatgcta
     1141 tccacatcac tggtggaaac aattcattaa tactccatga aggttctgtc attactggtg
     1201 atgtacaggt taacaattca tccattctga aaattatcaa caatgattac actgggacca
     1261 caccaactat tgaaggtgat ttatgtgctg gtgattgtac aactgtttca ctatcaggta
     1321 acaaattcac tgtttcaggt gacgtttctt ttggtgagaa cagttcttta aatttagctg
     1381 gaatcagtag tctggaagct tctggaaata tgtcatttgg caacaatgta aaagtggagg
     1441 ctattataaa taactgggcg cagaaggact ataaactgct aagtgcagat aaagggataa
     1501 caggtttcag tgtttctaat atatctatca tcaatccgtt actcactact ggtgctattg
     1561 actatacaaa aagctatatc agtgaccaga ataaattgat ctacggtttg agctggaatg
     1621 atacagatgg cgacagtcat ggagagttca atctgaaaga aaacgctgaa cttactgtta
     1681 gtactattct ggcagataat ctcagccatc ataatataaa tagctgggac ggaaaatccc
     1741 taacaaaatc aggggaggga actctcattt tggcggaaaa aaatacctac tctggtttca
     1801 ccaacatcaa tgcaggcatt ctaaaaatgg ggacagttga agctatgaca cgtaccgctg
     1861 gtgttattgt taataaaggt gctaccttga atttttcagg catgaaccaa actgttaaca
     1921 ctttattaaa tagtgggact gtgctaatca ataatattaa tgcccctttt ttgcctgacc
     1981 ccgtcattgt cacaggtaac atgactctgg agaaaaacgg tcatgttatt ctcaataata
     2041 gttcgtcaaa tgtcggtcag acctatgttc agaaaggtaa ttggcatgga aagggcggaa
     2101 tattatcttt gggcgcggtt ctcggcaatg acaacagtaa aactgaccgg ctggaaattg
     2161 caggccatgc gtctggtatt acctatgttg cagtgacaaa tgagggaggc tctggagata
     2221 aaactcttga aggtgttcaa attatttcga cagattcttc tgataagaat gcttttattc
     2281 agaaaggccg tattgttgct ggtagttatg actatcgcct gaaacagggc actgtatctg
     2341 gactgaatac caataagtgg tatctaacta gtcagatgga taatcaagaa tcaaaacaga
     2401 tgagcaatca agagtctact caaatgagta gtcgccgagc tagttcacag cttgtatctt
     2461 cacttaattt gggtgaaggt agtattcaca catggcgccc tgaagctggc agttatattg
     2521 ctaacctgat agcaatgaac acgatgttta gtccttctct ctatgaccga cacggtagca
     2581 ctattgttga tcctactaca ggtcagctca gcgaaaccac catgtggatt cgtactgttg
     2641 gtggacataa tgagcataat ttagctgata gacaattaaa aaccacagct aacaggatgg
     2701 tttatcagat tggtggagat attttgaaga caaacttcac tgatcatgat ggcttgcatg
     2761 tgggtattat gggagcttat ggatatcagg atagcaaaac tcataataag tatactagtt
     2821 atagttcacg aggaactgtg agcggttata ctgccggttt gtacagttct tggtttcagg
     2881 atgaaaaaga acgaacaggt ctatatatgg atgcttggtt gcagtacagt tggtttaata
     2941 atacagtcaa aggagatggg ttaactggtg agaaatattc cagcaaagga ataacaggag
     3001 ctttggaagc tggctatatc tacccaacca tacgctggac tgctcataat aatattgaca
     3061 acgcattgta tctcaatcca caagtccaga taactaggca tggggtaaaa gcaaacgact
     3121 atattgaaca caatggcact atggtcacat cctctggggg caataatatt caagcaaaat
     3181 tgggattgcg tacatcctta attagtcaga gttgtatcga taaggagact cttcgtaagt
     3241 tcgaaccatt tttggaagtg aattggaaat ggagctcaaa gcaatatggt gtaattatga
     3301 atggcatgtc aaatcaccag ataggcaacc gtaatgtgat tgaactcaaa actggtgtgg
     3361 gggggcgtct tgcagataac ctaagcatct ggggaaacgt atctcagcaa ttgggtaata
     3421 acagttacag agacacccaa ggtattttgg gtgtgaaata taccttctga taataaaaat
     3481 gcagggcgtg ttgatgtcct gcatttctct gggacccacc gtgtaaaagc tcggtgggtt
     3541 tttgtgcaca tgatggtatg cctgcgatca atgccaaacg tgtttaccag atcatgcgcc
     3601 agaatgcgct gttgcttggc gttacacagg aattgcatta gaaagcgatg aacgaatgct
     3661 accgccaaat attcgtggtt atgcgcctca gattactggt attgcagaaa ctaatgcacg
     3721 tgttgttgta tctcaacaag gtcgagtaat atatgactct actgtaccag ctggaacttt
     3781 ttctattcag gacttgagta gttctgtacg aggaatattg gatgttgaaa tttttgagca
     3841 aaatggtaaa agaaaacatt tccaagtaga aatgtgcagg tgtgcctttc ttatccagac
     3901 ttggtcagag taggtataaa ttagtaacag gtctacctaa aacaaataat aaagcaactg
     3961 gtgatgcatt tttttcggtg aagatcctac ggggtcctga gcccggtgaa gtggcgaggc
     4021 aaatcacatg gggaagcgta cagcctgaa
//
LOCUS       DAZSIO010000243         4024 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZSIO010000243 DAZSIO010000000
VERSION     DAZSIO010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.247"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(139..3654)
                     /locus_tag="ACHX5T_003892"
     CDS             complement(139..3654)
                     /locus_tag="ACHX5T_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR8468864.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
BASE COUNT         1037 a         1019 c          840 g         1128 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 actt
//
LOCUS       DAZSIO010000244         3975 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZSIO010000244 DAZSIO010000000
VERSION     DAZSIO010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.248"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(322..1971)
                     /gene="pgi"
                     /locus_tag="ACHX5T_003893"
     CDS             complement(322..1971)
                     /gene="pgi"
                     /locus_tag="ACHX5T_003893"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR8468865.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2208..2404
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2496..3845
                     /gene="lysC"
                     /locus_tag="ACHX5T_003894"
     CDS             2496..3845
                     /gene="lysC"
                     /locus_tag="ACHX5T_003894"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR8468866.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
BASE COUNT          937 a          988 c         1039 g         1011 t
ORIGIN      
        1 taagtttcaa cttatattta aataacaata aaaatcataa gcttaaaata aaaaacaaaa
       61 gtaattaatc tgcgtattct ccgaaataaa caacgattaa aaatctattt taggattaac
      121 ctcacggtat gatttccgtt aaatcacaga caaggggatt tatctgataa aacaatgccg
      181 acttattgtc ggcattgttt ttaaggttat ttaactcctc atactatgca ttaatgtcga
      241 tattggcatc aggctccggt tgccggatgc ggcgtaaacg ccttatccgg cctacatatc
      301 ggcatcaggc acgcatgatg attaaccgcg ccacgcttta tagcggttaa tcagaccatt
      361 ggtcgagcta tcgtggctgc tgatttcttt atcatctttc agctctggca gaatacggtt
      421 tgccagttgt ttacccagct ccacgcccca ctggtcgaag gtgaagatgt tcaggatcac
      481 gccctgagta aagattttgt gctcatacag cgcaatcaac gcacccaggc tgaacggagt
      541 gatttcgcgc agcaggatgg agttggtcgg gcggttacct tcgaacactt tgaacggcac
      601 cacgtagtca agcgttgccg gatctttacc ctgatcgcga tattcctgct caaccacttc
      661 gcgggattta ccaaacgcca gcgcttcggt ctgggcgaag aagttagaca gcagtttctg
      721 gtggtgatca gagagcgggt tatgggtgat agccggggcg atgaaatcgc acggtaccat
      781 tttggttccc tggtggatca gctggtagaa cgcgtgctga ccgttagtgc ctggttcacc
      841 ccagataatc gggccagtct ggtaatccac aacgttaccg ttacggtcaa catacttacc
      901 gttggactcc atattgccct gctggaagta cgccgcgaaa cggtgcatat actggtcata
      961 cggcagaatc gcttcagttt ccgcaccaaa gaaattgttg taccagatgc caatcagcgc
     1021 cagcaggaca ggcaggtttt tctcggcagg cgtggtggag aaatgcttgt ccattgcgtg
     1081 tgcgccagaa agcagttcaa cgaagttatc aaagccgatg gagagaacaa tcgacaggcc
     1141 aatcgctgac cacaaagagt aacggccgcc gacccagtcc cagaactcga acatgttggc
     1201 agtatcaata ccaaactcgc caacggcttt ggcattggtt gagagcgccg caaaatgttt
     1261 cgcaacgtgt ttttcatcac ctgccgcttt caggaaccag tcacgcgcgc tatgggcgtt
     1321 ggtcatggtt tcctgagtgg tgaaggtttt agatgctacc aggaacagcg tggtttccgg
     1381 gtttactttt ttcagcactt ccgagatgtg agtcccatcg acgttagaaa caaagtgcat
     1441 gttcaggtgg tttttgtacg gacgcagtgc ttcggtcacc atgtacgggc cgaggtcaga
     1501 accgccgatc ccgatgttca ctacgtcagt gattgctttg ccggtataac ccttccactc
     1561 accggagata atcgcttccg agaaggtttt catcttctcc agcaccgcgt tgacttctgg
     1621 cataacatct ttgccatcaa ccagaatcgg ggtattgcta cgattacgca gcgctacgtg
     1681 cagcacggcg cggttttcag tgcggttgat cttctcgcca gagaacatcg acttaatcgc
     1741 gcccgccaga tcgcactctt tcgccagatc ctgtaatttc gccagcgtct cttcagtgat
     1801 gcggtttttg gagtaatcca ccagcatctg atcgtcgaag gttgcggaga acttagaaaa
     1861 acgatcgccg tctttagcaa aaagatcggc gatcgtaacg tctttcattt catcgaagtg
     1921 tttctgtagt gcctgccagg cagcggtctg cgttggattg atgtttttca ttagcaatac
     1981 tcttctgatt ttgagaattg tgactttgga agattgtagc gccagtcaca gaaaaatgtg
     2041 atggttttag tgccgttagt gtaatgttga gtgtaaaccc ttagcgcagt gaagcattta
     2101 ttagctgaac tactgaccgc caggagtgga tgaaaaatcc gcatgacccc gtcgttgaca
     2161 accgccccgc tcacccttta tttataaatg tactacctgc gctagcgcag gccagaagag
     2221 gcgcgttgcc caagtaacgg tgttggagga gccagtcctg tgataacacc tgagggggtg
     2281 catcgccgag gtgattgaac ggctggccac gttcatcatc ggctacaggg gctgaatccc
     2341 ctgggttgtc accagaagcg ttcgcagtcg ggcgtttcgc aagtggtgga gcacttctgg
     2401 gtgaaaatag tagcgaagta tcgctctgcg cccacccgtc ttccgctctt cccttgtgcc
     2461 aaggctgaaa atggatcccc tgacacgagg tagttatgtc tgaaattgtt gtctccaaat
     2521 ttggcggtac cagcgtagct gattttgacg ccatgaaccg cagcgctgat attgtgcttt
     2581 ctgatgccaa cgtgcgttta gtagtcctct cggcttctgc tggtatcact aatctgctgg
     2641 tcgctttagc ggaagggctg gaacctggcg agcgattcga aaaactcgac gctattcgca
     2701 acatccagtt tgccattctg gaacgtctgc gttacccgaa cgttatccgt gaagagattg
     2761 aacgtctgct ggagaacatt actgttctgg cagaagcggc ggcgctggca acgtctccgg
     2821 cgctgacaga tgagctggtc agccacggcg agctgatgtc gaccctgctg tttgtcgaaa
     2881 tcctgcgcga acgcgatgtt caggcacagt ggtttgatgt acgtaaagtg atgcgtacca
     2941 acgaccgatt tggtcgtgca gagccagatg tagccgcgct ggcggaactg gccgcgctgc
     3001 agctgctccc acgcctcaat gaaggcttag tgatcaccca gggatttatc ggtagcgaaa
     3061 ataaaggtcg tacaacgacg cttggccgtg gaggcagcga ttatacggca gccttgctgg
     3121 cggaggcttt acacgcatct cgtgttgata tctggaccga cgtcccgggc atctacacca
     3181 ccgatccacg cgtagtttcc gcagcaaaac gcattgatga aatcgcgttt gccgaagcgg
     3241 cagagatggc aacttttggt gcaaaagtac tgcatccggc aacgttgcta cccgcagtac
     3301 gcagcgatat cccggtcttt gtcggctcca gcaaagaccc acgcgcaggt ggtacgctgg
     3361 tgtgcaataa aactgaaaat ccgccgctgt tccgcgcgct ggcgcttcgt cgcaatcaga
     3421 ctctgctcac tttgcacagc ctgaatatgc tgcattctcg cggcttcctc gcggaagttt
     3481 tcggcatcct cgcgcggcat aatatttcgg tagacttaat caccacgtca gaagtgagcg
     3541 tggcattaac ccttgatacc accggttcaa cctccactgg cgatacgttg ctgacgcaat
     3601 ctctgctgat ggagctttcc gcactgtgcc gggtggaggt ggaagaaggt ctggcgctgg
     3661 tcgcgttgat tggcaatgac ctgtcaaaag cctgcggcgt tggcaaagag gtattcggcg
     3721 tactggaacc gttcaacatt cgcatgattt gttacggcgc atccagccat aacctgtgct
     3781 tcctggtgcc cggcgaagat gccgagcagg tggtgcaaaa actgcatagt aatttgtttg
     3841 agtaaatact gtatggcctg gaagctatat ttcgggccgt attgattttc ttgtcactat
     3901 gaggtagcct gagtttaacg gacactcctt cctgaaatag aatggcatca gaaggagcta
     3961 ataatgagca gaaaa
//
LOCUS       DAZSIO010000245         3962 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZSIO010000245 DAZSIO010000000
VERSION     DAZSIO010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.249"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..428)
                     /locus_tag="ACHX5T_003895"
     CDS             complement(<1..428)
                     /locus_tag="ACHX5T_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFR8468867.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNY"
     gene            complement(431..886)
                     /locus_tag="ACHX5T_003896"
     CDS             complement(431..886)
                     /locus_tag="ACHX5T_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFR8468868.1"
                     /translation="MIQFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEVRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            complement(886..1734)
                     /locus_tag="ACHX5T_003897"
     CDS             complement(886..1734)
                     /locus_tag="ACHX5T_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFR8468869.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERISQAESTLQEHE
                     TRIAQNESDIASLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGTLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            complement(1734..3152)
                     /locus_tag="ACHX5T_003898"
     CDS             complement(1734..3152)
                     /locus_tag="ACHX5T_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFR8468870.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDGINYGCEQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGCQIRIE"
     gene            complement(3153..3653)
                     /locus_tag="ACHX5T_003899"
     CDS             complement(3153..3653)
                     /locus_tag="ACHX5T_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFR8468871.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDVSPGINVGYIFADADVAPEPGDEHGLSNNAINAVIFNLACRIAPDYALEAP
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGGE"
     gene            complement(3631..3891)
                     /locus_tag="ACHX5T_003900"
     CDS             complement(3631..3891)
                     /locus_tag="ACHX5T_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468872.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFAHPDDL
                     LKDVAEPEPEPEPEPEPEPEEKQRKKPGRKPKAAADEPDNEG"
BASE COUNT          914 a         1037 c          970 g         1041 t
ORIGIN      
        1 ttgtaattgc tcatctggtt tgataaccac gactgaccga gagtcggagc aatcgtagcc
       61 agttgattgc ttgtggctgt cgaaccaagt cctcccgtag cctccgcagc agcaagactc
      121 tggtaacgag cctgacctgc aaggtcttta tactgctgag agttgtaata ctgattaagt
      181 gcctgaccct gtccttctaa actggaaagg ttctgaagct ggttaacata ctgctccgca
      241 agcggcgtga acggagcaag gtttttcatg atcgtctgcc actgctgatt ttgcaggtct
      301 gcggcatact tctgagcttc tgctgcatac tttgcacttt tatcagagct gccacctttc
      361 ccgccttttt cagggcaata aggttcctcg ccgcgcagtt ttctgcccag cttaaatgca
      421 tataacatgg ctatctcccg tgattcagga agtcgattag ttcttcgcgt gtagcactgt
      481 aaaatgtcac gtcatccacg cctttgaagt atttcttgat ggttcctact cgcttaaggc
      541 caatcattgc gcagtacatc tgaccgtggc ggaatttgcg cgcagcgaac gatgtgacgc
      601 actgaacggt ggtgttagtc agaatgtatc gccagaacgc cagcccgatt tccttgctga
      661 atccacgaac ctctggcagg tacatggcgt ggcaatcaaa ggtcaaaggc tgaatctcct
      721 gatagtaaac aatgccgccg aactgcccgt gcacgttcac ctcgaagtaa cggcattcag
      781 gcttgtagtc gtatccatca ccgttgttgc tcccggcgat aatgtcgggg tgatttccca
      841 cggcttctat caggtcgatg tttcgcgttg gtttgaactg aatcatcact gctccgcgat
      901 tatcttgatg gttgtggcag taaacgccgc accattcgac tgaatggtta acgtactgcc
      961 atttgtggca agaaatccgt ctttatccac gctgaagaat gtagctaaca ggatgttgtc
     1021 ggttgttgtc gccgcattac gactgctgac cagcgtatca ggaacagagc cggaaaaggt
     1081 tagctgcatt gacctgttgg cggttccgct gggccacgtc ccgacgatcg acagcttgaa
     1141 gaacaaggtt ttgttctcgt tgaacacaac catcttgttg ttaacggtgt cgaagaatgg
     1201 tgccaacgtg ccggatgacg gcgtgagcgt tttcagtagg ctaacaaggt tggtcggcgc
     1261 tgtcgggatg gttacagata cgccagagta aacaacctct gacttcttgc gagtagtggc
     1321 atactccaga gcatcgatgc gcgtttcatg gtctgaaacc tgcgattcca gcgactgaac
     1381 tctggtatca agcgacgcaa tatcgctttc attctgagcg attcgcgttt catgttcctg
     1441 aagagttgat tctgcctggc tgattcgctc ctcatgatta acaagcgttg cttccgcagc
     1501 agaaattcgc tgctcatggt cagcgagaat cacatcctgc tcatcgttcc tgacttgtgc
     1561 gtcataagcg ccctgtccgg cctcgttggc cttgttggcc acgttaccaa catcagtgcc
     1621 ctgtgcgata acgtaaagca gatacgactg cgagaagata ttgcgtggaa ggactgatgt
     1681 gtcgagccgt gtagcctgaa tgattaccgg cacattgaga ttcgaatccg ccattactca
     1741 atccttatct ggcagccaga cagagtgaca ggtgacttcg tgataacgcg caatttgaag
     1801 ccgacatttt tcctgatgcg ccctactcgc ttccacaaaa cgcgtttgtc gtaaacgaac
     1861 ggttcattct gctcaatcat ctgctcacac ccgtaattga tgccgtcagt ggttgcagag
     1921 agaaaaaggc ggtcagcata ctgcgcaacg ccagttgacg attcaacctc aaggtcgaac
     1981 actctggcgt tatccgcttt gaacagtgga gtaaacagca ggtgttcctg ttgcttgtcg
     2041 tactggctgc tgatgtcgaa ttgcaatttc ccggtcacgg actccagctt atcgccgcac
     2101 gttatttgat tgccttcgta aatgaagtcg atagcgcggt acacatcgtc atacaggcct
     2161 gttttcagca cacaccattg cggaccattg gcgcttgaag atgcgtcgta caccagtaca
     2221 tggcgcggaa ggtggataat cagcaactca tgcgcatcaa accgcagcga ttccatcacg
     2281 ccatcagcca gttcatcagc agtgtaggag cggaggattt tctcaatgct cgcgctggcg
     2341 attggtgaca cctgaccgga gccgatgatg tatacagacg gcgcacctgt tgccggattg
     2401 ctgatgaacg cataagaatc agcgaatggt gttttgcagt aagtcccggc aatacctttc
     2461 tgcaccatca gcgatggctg tgcgacatac aaagcggcac caacggtggt tgcacctgtc
     2521 agggaaaaat attcaatcgt cgatgagcca aagcagacga tgaagtctcg ccatgtacct
     2581 atgccgatga tgccgtccgg ctgagactcg gcacgatatt gtgcactgta gcggtcagga
     2641 tgcgattcat cttcaaggtc agtgataaac catgaatcag tgccgtcttt tgaccacgca
     2701 taacgcccac gtaagcgcgt aatgtcgcga actgaaccta actcatactg agtgaatcca
     2761 ctgtctgtag gccagtttga gacggtttta accgtgccat catagcgata ctcgaccagt
     2821 tgaccattaa cgcctaccgc ctgtgatgtc cgaccatgcg ccattgatac gcgaccactt
     2881 ccggcaacat caccgacctc gctttctccc ttatacaact tgccaccgca aacgcgataa
     2941 acagcattct gcgccatgtt gtactcgaca ccgcgcgata caccattcac atcagaacgt
     3001 ttggcaatgc ccgggaatga gcgaagatat ccgctgctgt tgagtatttc tttgggtgta
     3061 gccagcatat tcactggcag atagtcgatg tagtcggcgt ttcggaaatc tttgccgaca
     3121 cctttcataa gcggaagttg ctgaatcggc atttattcgc ccccgttatc gcaaggttcc
     3181 ttccggtgga agtaattcca accattccac ttcgccaact ggttaccgct accaacaggc
     3241 atacggtttg gataaccgga cttacattta gcggcttttg ctctgtccat tgcagacagt
     3301 ttgacgagtc gctctttccc gtatctggca gtggttataa gttttgctgg cgcttccagc
     3361 gcataatctg gagcaatgcg gcaggcaagg ttgaaaatga cggcattgat agcgttattt
     3421 gataaaccgt gctcatcgcc cggttctgga gcgacatctg catcagcgaa aatgtagcca
     3481 acgttgatac caggtgacac atcaccgcca agccattcag ccatcatcat ttcaaggtcg
     3541 ttgacgccgt cttccataga ctgcggttct acatcggtta acgtggcatt tgatgccaca
     3601 ccgagcttac gtaatgccgc aagaactaaa tcacccttcg ttgtcaggtt catctgctgc
     3661 cgccttaggt tttcgaccag gctttttacg ctgtttttct tctggctctg gctctggctc
     3721 tggctctggc tctggctctg gctctgcaac atccttcaga aggtcatcgg gatgtgcaaa
     3781 ccagccagca tccagatatt cctgaagctc ttcggctttc acgatttcaa agtcgtatcc
     3841 aacgcctttc cacttcttca tgtcgccatg acgaaagatc atgtgtgtca tgtttgtctc
     3901 cagataaaaa agggagccga agctccctct ggttatcacg cagtctggtt aggcagacca
     3961 ac
//
LOCUS       DAZSIO010000246         3931 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZSIO010000246 DAZSIO010000000
VERSION     DAZSIO010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.250"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            72..302
                     /locus_tag="ACHX5T_003901"
     CDS             72..302
                     /locus_tag="ACHX5T_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468873.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            280..780
                     /locus_tag="ACHX5T_003902"
     CDS             280..780
                     /locus_tag="ACHX5T_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFR8468874.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            780..2198
                     /locus_tag="ACHX5T_003903"
     CDS             780..2198
                     /locus_tag="ACHX5T_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFR8468875.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            2198..3046
                     /locus_tag="ACHX5T_003904"
     CDS             2198..3046
                     /locus_tag="ACHX5T_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFR8468876.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            3046..3501
                     /locus_tag="ACHX5T_003905"
     CDS             3046..3501
                     /locus_tag="ACHX5T_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFR8468877.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            3504..>3931
                     /locus_tag="ACHX5T_003906"
     CDS             3504..>3931
                     /locus_tag="ACHX5T_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFR8468878.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNY"
BASE COUNT         1034 a          957 c         1013 g          927 t
ORIGIN      
        1 gttggtctgc ctaaccagac cgcgtgataa ccagagggag cttcggctcc cgtttttatc
       61 tggagacaag catgacacac atgatctttc gtcatggcga catgaagaag tggaaaggcg
      121 ttggctacga ctttgaaatc gtgaaagccg aagagcttca ggaatatctg gatgctggct
      181 ggttttcaca tcctgatgac cttttgaagg atgttgcaga gccagagcca gaagaaaaac
      241 agcgtaaaaa gcctggtcga aaacctaagg cggcagcaga tgaacctgac aacgaagggt
      301 gatttagttc ttgcggcatt acgtaagctc ggtgttgcat caaatgccac gttaaccgat
      361 gtcgaaccgc agtctatgga agacggcgtc aacgaccttg aaatgatgat ggctgaatgg
      421 cttggcggtg atgcgtcacc tggtatcaac gttggctaca tttttgctga tgcagatgtt
      481 gctccggatc cgggcgatga gcacggttta tcaaataacg ctatcaatgc cgtcattttc
      541 aaccttgcct gccgaattgc tccagattat gcgctggaag cgtctgcaaa acttataacc
      601 actgccagat acgggaaaga gcgactcgtc aaactgtctg caatggacag ggcaaaagca
      661 gctaaatgta agtccggtta tccaaaccgt atgcctgttg gtagtggtaa ccagttggcg
      721 aagtggaatg gttggaatta cttccaccga aaggaacctt gcgataacgg gagcgaataa
      781 tgcctattca gcaacttccg cttatgaaag gtgtcggcaa agacttccga aacgccgact
      841 atatcgacta tctgccagtg aatatgctgg ctacacccaa agaaatcctg aacagcagcg
      901 gatatcttcg ctcattcccg ggcattgcca aacgttctga cgtgaacggt gtatctcgcg
      961 gcgtcgagta caacatggcg cagaatgctg tttatcgcgt gtgtggcggc aagctgtaca
     1021 aaggagaaag tgaagtcggt gatgttgccg gaagtggtcg tgtatcaatg gcgcatggtc
     1081 ggacatcaca ggcggtaggc gttaatggtc aactggtcga gtatcgctat gatggcacgg
     1141 tcaaaaccgt ctcaaactgg cctacagaca gtggattcac tcagtatgag ttaggttcag
     1201 ttcgcgacat tacgcgctta cgtgggcgtt atgcgtggtc aaaagacgga actgattcat
     1261 ggtttatcac tgaccttgaa gacgaatctc atcctgaccg ttacagcgca caatatcgtg
     1321 ccgagtctca gcctgacggc atcatcggta tcgggacatg gcgagacttc atcgtctgct
     1381 ttggttcatc gactattgaa tatttctccc tgactggcgc aaccaccgtt ggtgccgctt
     1441 tgtatgttgc acaaccatcg ctgatggtgc agaaaggcat tgccggaact tactgcaaaa
     1501 cgccattcgc tgattcgtat gcgtttatca gcaatccggc aacgggtgcg ccgtctgtgt
     1561 atatcatcga ctccggtcag gtgtcaccaa ttgccagcgc gagcattgag aaaatcctcc
     1621 gctcctacac tgctgatgaa ctggctgatg gtgtgatgga atcgctgcga tttgatgctc
     1681 atgagttgct gattatccac cttccgcgtc acgtcctcgt gtacgacgca tcttcaatcg
     1741 ccaatggccc acaatggtgt gtgctgaaaa caggtctgta tgacgatgtg taccgcgcta
     1801 tcgacttcgt ttacgaaggc aatcagataa cgtgcggcga taagctggaa tctgttaccg
     1861 ggaaactgca attcgatatc agcagccagt acgacaagca acaggaacac ctgctgttta
     1921 ctccactctt caaagcggat aacgccagag tgttcgacct tgaggttgaa tcgtcaactg
     1981 gcgttgcgca gtacgccgac cgcctttttc tctctgcaac cactgactgc atcaattacg
     2041 ggcgtgagca gatgattgag cagaatgaac cgttcgttta cgacaagcgt gttttgtgga
     2101 agcgagtagg gcgcatcagg aaaaatgtcg gcttcaaatt gcgcgttatc acgaagtcac
     2161 ctgtcactct gtctggcgct cagataagga ttgagtaatg gctgattcga atctcaatgt
     2221 gccggtaatc atccaagcta cgcggctcga tacatcagtc cttccacgca atatcttctc
     2281 gcagtcgtat ctgctttacg ttatcgcaca gggcactgat gttggtaacg tggctaacaa
     2341 agccaacgag gccggacagg gcgcttatga tgcacaggtc aggaacgatg agcaggatgt
     2401 gattctcgct gaccatgagc agcgaatttc tgctgcggaa gcaacgcttg ttaatcatga
     2461 ggagcgaatc agacaggcag aatcaactct tcaggaccat gaaacacgaa tagctcagaa
     2521 tgaaagcgat atttcgtcgc ttgacacaag agttcagtcg ctggagtcgc aggtttcaga
     2581 ccatgaaacg cgcatcgatg ctctggagta tgcaaccaca cgcaagaagt cagaggttgt
     2641 ttactctggt gtatctgtaa ccatcccgac agcgccgacc aaccttgtta gcctgctgaa
     2701 aacgctcaca ccgtcatccg gctcgttggc accattcttc gacaccgtta acaacaagat
     2761 ggttgtgttc aacgagaaca aaaccctgct cttcaagttg tcgattgtcg gcacgtggcc
     2821 cagcggaacc gccaacaggt caatgcagct aaccttttcc ggctctgttc ctgacacact
     2881 ggtcagcagt cgtaatgcgg cgacaacaac cgacaacatc ctgttagcta cgttcttcag
     2941 cgtggataaa gacggctttc ttgccacaaa tggcagtacg ttaaccattc agtcgaatgg
     3001 tgcggcgttt actgccacaa ccatcaagat aatcgcggag cagtgatgat tacattcaaa
     3061 ccaacgcgaa acatcgacct gatagaagca gtcggaaatc accctgacat tatcgccggg
     3121 agcaacaacg gtgatggata cgactacaaa cctgaatgcc gttactttga ggtgaacgtg
     3181 cacggtcagt ttggcggcat tgtttactat caggagattc agccgctgac attagattgc
     3241 cacgccatgt acctgccaga gattcgcggc ttcagcaagg aaattgggct ggcgttctgg
     3301 cgatacattc tgactaacac caccgttcag tgcgtaacat cgttcgctgc acgcaaattc
     3361 cgacacggtc agatgtactg cgcaatgatt ggccttaagc gtgtaggaac catcaagaaa
     3421 tacttcaaag gcgtggatga cgtgactttt tacagcgcca cacgcgaaga actaatcgac
     3481 ttcctgaatc acgggagata gccatgttat atgcatttaa gctgggcaga aaactgcgcg
     3541 gcgaggaacc ttattgccct gaaaaaggcg ggaaaggtgg cagttctgat aaaagcgcaa
     3601 agtatgcagc agaagcccag aagtatgctg cagacctgca aaatcagcag tggcagacga
     3661 tcatgaaaaa ccttgctccg ttcacgcctc ttgcggagca gtatgttaac cagcttcaga
     3721 acctttccag tttagaaggt caggggcagg cacttaatca gtattacaac tctaagcagt
     3781 ataaagacct tgcagggcag gcgcgttacc agagtcttgc tgctgcggag gcgacgggtg
     3841 gacttggttc gacagccaca agcaatcaac tggctacgat cgcgccgaca ctcggtcagt
     3901 cttggttatc aaatcagatg agcaattaca a
//
LOCUS       DAZSIO010000247         3859 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZSIO010000247 DAZSIO010000000
VERSION     DAZSIO010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.251"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..70
                     /locus_tag="ACHX5T_003907"
     CDS             <1..70
                     /locus_tag="ACHX5T_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468879.1"
                     /translation="DRLNNNEQDMFNIISDKIQELF"
     gene            394..504
                     /locus_tag="ACHX5T_003908"
                     /pseudo
     CDS             394..504
                     /locus_tag="ACHX5T_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            582..896
                     /locus_tag="ACHX5T_003909"
     CDS             582..896
                     /locus_tag="ACHX5T_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468880.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            1178..1744
                     /gene="ipgB2"
                     /locus_tag="ACHX5T_003910"
     CDS             1178..1744
                     /gene="ipgB2"
                     /locus_tag="ACHX5T_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HFR8468881.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2006..2128)
                     /locus_tag="ACHX5T_003911"
                     /pseudo
     CDS             complement(2006..2128)
                     /locus_tag="ACHX5T_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            2271..2825
                     /locus_tag="ACHX5T_003912"
                     /pseudo
     CDS             2271..2825
                     /locus_tag="ACHX5T_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            3164..3525
                     /locus_tag="ACHX5T_003913"
                     /pseudo
     CDS             3164..3525
                     /locus_tag="ACHX5T_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            3612..>3859
                     /locus_tag="ACHX5T_003914"
     CDS             3612..>3859
                     /locus_tag="ACHX5T_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8468882.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
BASE COUNT         1133 a          837 c          864 g         1025 t
ORIGIN      
        1 agacagactt aacaataatg aacaagacat gtttaatatt atttctgaca aaatacaaga
       61 gttgttttaa gtgtgctaaa tagctgcaaa gaattgcact tagaaggatc tctccccgaa
      121 ttgtgtgatc tgcttaattt ctaaaaagaa acaaacagtt aactagactg agcgatacta
      181 cccatagaac cagtttccca tgacgaacga cactggacgc aaaaacaatc ataaaaaaca
      241 caataaatgt aatgaacaca tcccacccta ttaagtaaag tgcactgtga tagaggaagg
      301 gaaatacagg aaggtgtgga gccttgcaga tttctggcat catagtgccc aatacggaat
      361 gcataaagcc ctctcagact ggagactcca caaatgaaat atacagtgtt taattatatc
      421 aagtgcgatt acaatcgctg acgacggcac agttggtgtg gaggtctcag tcctgaacaa
      481 tttgaaaacc agaacctcac ttaagcctgt gttcatatta cgtgggtagg atcatacttt
      541 tatagaagct gtcagcggct acgtcccagc caaatcactt catgctccag cgccagagag
      601 gcaaggttgg gtttgctcag ctgccggttg attttgttgc gattgaaccg gacagcgtcc
      661 agggcgtcgg caaaagagcg aatctcacca atcgttgttt cattatccgc attaacgata
      721 gcttcaagaa acgccaaggt tttatcgaat ttatcagcaa cagtggctcc gggcataccg
      781 tgaccgttta tacaaaacgg cgttttcagc gtggagtttt catgaatagc ctcaacacga
      841 atgtcgtgaa gccagtgatg tatcggctac gatcctcagc tgatgccaga ccataggact
      901 gacactacga acagcaattt taatgacgta aatcttcata actacaacta acagggcatc
      961 attatgaatg atgccggtta ttttaccgct gttggtccct tcatttaggg actgccccaa
     1021 gttttttcat actctctttc tcagagagtt tagtctctgg gattccagac ggtccatcat
     1081 tgagccaagg gagtaaagaa ctcaaagcat ttacagaatt gtttgaatca gctaaaataa
     1141 tgacaatgtt actattttac atgacaaggt gattataatg cttggaacat cttttaataa
     1201 ttttggaatc agtttatctc acaaaagata tttttcagga aaggtggatg agattatccg
     1261 ctgtacaatg gggaaaagga tagtaaaaat atcatcaacc aaaataaata ctagtatttt
     1321 gagctctgtt tcagaacaaa tcggtgaaaa tattacagat tggaaaaatg atgaaaaaaa
     1381 agtctacgta tcccgggtgg taaaccaatg tattgataag ttttgtgcag aacacagccg
     1441 aaaaatcggc gataatctcc gtaaacagat atttaaacag gttgagaaag attatcgcat
     1501 ttctctagac atcaatgctg cacaaagttc cataaatcat cttgtatcag gaagcagtta
     1561 tttcaaaaag aaaatggacg aactttgtga agggatgaat agaagcgtta aaaatgatac
     1621 aacaagcaat gttgcaaatc ttatttccga tcagtttttt gagaaaaatg tgcaatatat
     1681 tgatctaaag aaattacgtg ggaatatgag tgactatatt acaaatttag aatcgccttt
     1741 ctgatgacag aacatcccga gtaaagaaac taagaaaagt aaatacccag gaattgcctg
     1801 agcggtgcta tatttctcct cgcaagtaag tcctaatata ttgaattgtt gtatagccag
     1861 tttttgtatt ttcctagctc atggatgtgg gatccctctc ccctttctct gctcaaaggt
     1921 aagcggctcg tcagaatcat attgatattt actgagaagt cagatcaact ttccaaggca
     1981 acagatcgcg tacccggttt gccggtcagt cctggatatg ttcaataacg taacgcagcc
     2041 atttttctgg ctccacattg ttcagacggc atgtgccgat cagcgagaac aacaccgctg
     2101 catgttcgtc accgctgtcg gaacctgcga gtcgagtcag ccggagagag tttcactctc
     2161 cggctgctta cggaaccgta cgtgacactc tcgcgtcata cggctcttga tcactcattc
     2221 gatgcacgta gtaaatacca gtgagcaaat tgatcttacc cagcaatacg gtcagttacc
     2281 gcgcacatgg cctgcctgtg tcagaaaatc tgctggagca ggatttttac gccagtggtc
     2341 cgaaccagaa gtgggcggga gacatcacgt acttacgtac agatgaaggc tggctgtatc
     2401 tggctgtggt cattgacctg tggtcacgtg ccgttattgg ctggtccatg tcaccacgcc
     2461 tgacggcgca actggcctgc gatgccctgc agatggcgct gtggcggcgt aagagacccc
     2521 ggaacgttat cgttcactcg gaccgaggaa gtcagtactg ttcagcagat tatcaggcgc
     2581 tgctgaagtg gcataatctg cgtggcagta tgagcgcaaa gggctgctgc tacgataatg
     2641 cctgcgtgga aagcttcttt cattcgctga aagtggaatg tctccacgga gaacacttta
     2701 tcagccggga aataatgagg gcaacagtgt ttaattatat cgaatgtgat tacaatcgct
     2761 ggcgacggca cagttggtgt ggcggcctca gtccggaaca atttgaaaac cagaacctcg
     2821 cttaggcctg tgtccatatt acgtgggtag gatcacttac gccctgtggt tgaaacgtga
     2881 gcatattgtt ttcagatgct gttgtcttgc ttcagagtga tctgtcctcc ggatttacgc
     2941 aaagttatcc acaggcactg tgcatttaaa aaacttatct tttcttcttg attttaaaaa
     3001 ctcagatcgt catatcatta tgataaacat aataaaaaac tctaaacatg agaaggtttt
     3061 taacgtaagg tgagaggatt tttaagtgta tccatagatt aaaaatgaca atgcctccaa
     3121 ttagttgaat taggggttat aatacccgct tttaggttct ttcatggtca cagttaactt
     3181 tcattgcccc cgttgccagt ccgttcaggt ttaccgtcac gggcagaacc caaaaggaca
     3241 tgatcgattc cgttgccgtg actgtcaccg ggtgtttcag ctcacttact gttacgaggc
     3301 ccgtaaaccg ggagttaaaa accaaatcac tgaaatggct ttcaacggtg ccggagttcg
     3361 cgatactggc cgtacgctga aaatcggcat caataccgtc atacggacat taaaaagctc
     3421 ccgccccggc ggataacatc tgcgccagtc gctcacgctg atgttgccct tatctgtgaa
     3481 cttgatgggc agtggagctt cgttggcagc aaggcgcaac aacactgtca cgaacggtgc
     3541 aatagtgatc cacacccaac gcctgaaatc agatccaggg ggtaatctgc tctcctgatt
     3601 caggagagct tatggtcact tttgagacag ttatggaaat taaaatcctg cacaagcagg
     3661 gaatgagtag ccgggcgatt gccagagaac tggggctctc ccgcaatacc gttaaacgtt
     3721 atttgcaggc aaaatctgag ccgccaaaat atacgccgcg acctgctgtt gcttcactcc
     3781 tggatgaata ccgggattat attcgtcaac gcatcgccga tgctcatcct tacaaaatcc
     3841 cggcaacggt aatcgctcg
//
LOCUS       DAZSIO010000248         3841 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZSIO010000248 DAZSIO010000000
VERSION     DAZSIO010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.252"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            81..1415
                     /gene="adeQ"
                     /locus_tag="ACHX5T_003915"
                     /gene_synonym="yicO"
     CDS             81..1415
                     /gene="adeQ"
                     /locus_tag="ACHX5T_003915"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFR8468883.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1468..1920
                     /locus_tag="ACHX5T_003916"
     CDS             1468..1920
                     /locus_tag="ACHX5T_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFR8468884.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2131..3321
                     /gene="nepI"
                     /locus_tag="ACHX5T_003917"
     CDS             2131..3321
                     /gene="nepI"
                     /locus_tag="ACHX5T_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFR8468885.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3362..3655)
                     /gene="yicS"
                     /locus_tag="ACHX5T_003918"
     CDS             complement(3362..3655)
                     /gene="yicS"
                     /locus_tag="ACHX5T_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFR8468886.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
BASE COUNT          784 a          860 c         1018 g         1179 t
ORIGIN      
        1 atgtgataat tatcactgaa ccgttgttgt catttgtttt acaaaagcaa gggattaaat
       61 ctcctccaat ggacaaaaaa atgaataatg acaataccga ttacgtgagt aatgaatcag
      121 ggacgctttc gcgattattt aaactacctc agcatgggac caccgtccgc acagaattga
      181 ttgcggggat gaccactttt ttaaccatgg tgtacattgt ttttgtgaac ccgcaaatcc
      241 tcggcgcggc acaaatggac ccgaaagtgg tgtttgttac cacctgtttg attgccggta
      301 tcggcagtat tgcgatgggg atatttgcta acttacccgt ggcgctggct ccggcaatgg
      361 ggctgaacgc cttctttgcg ttcgtggtcg tgggggcgat ggggatctcc tggcagaccg
      421 ggatgggcgc gatattctgg ggcgcaattg ggctattttt gctcacgctg tttcgtatcc
      481 ggtactggat gatctccaac attccattaa gtttacgtat tggtatcacc agtggaatcg
      541 gattatttat tgccttaatg ggattaaaaa acactggtgt tattgtcgcc aataaagaca
      601 cgctggtgat gattggcgat ttaagttctc acggcgtgtt gttaggtatt ttagggtttt
      661 ttattataac cgtgttgtca tcacgtcatt ttcatgccgc ggtgctggtt tctattgtgg
      721 tgacgtcttg ctgtggatta tttttcggtg atgttcattt tagcggcgtc tattccattc
      781 cgcctgatat tagcggcgtc attggtgaag tagatttgag cggcgcgtta acacttgaac
      841 tcgccggtat cattttctcc tttatgctga tcaacctatt tgattcatca ggaacattaa
      901 ttggtgtaac tgataaagcg ggcttaatag atagcaacgg taaattcccc aatatgaata
      961 aggcgctgta tgttgatagc gtcagttcgg tggcaggcgc atttatcggt acctcgtctg
     1021 ttactgccta tattgaaagt acttctggtg tggcagtcgg tggtcgcacg gggctaacgg
     1081 ctgtcgtggt cggcgttatg ttcctgttag ttatgttctt ctcaccgctg gtggcgatgg
     1141 ttcctcctta cgcaaccgct ggagcgttaa tctttgttgg cgtgctgatg acttcgagcc
     1201 tggcgcgcgt taactgggat gattttaccg aatcggtgcc tgcgtttatt accacggtga
     1261 tgatgccctt tactttctcg atcaccgaag ggattgcact cggctttatg tcgtactgca
     1321 tcatgaaagt gtgcaccggg cgctggcgcg atctgaacct gtgtgtggtg gtggtcgcag
     1381 ctctgttcgc attgaagatt attctggtgg attagtaact ggcgagggtt tccagatatc
     1441 atgagttctg attacgcagg agaactcatg atctggataa tgctcgccac gctggcggta
     1501 gtgtttgtgg ttggttttcg ggtgctgaca tccggggcca gaaaagcgat tcgccgtctc
     1561 agcgatcggc tgaatatcga tgtcgtaccc gtggagtcga tggtcgatca aatgggaaag
     1621 tcagccggtg acgaattttt acgttatttg catcgcccgg atgagtcgca cctgcaaaac
     1681 gccgcgcagg tgttgcttat ctggcaaatt gtcattgtcg atgatagcga acagaacctg
     1741 ctgcaatggc accggatttt acaaaaagcc cgcctttccg cgccgattac cgatgctcag
     1801 gtcaggctgg cgctgggttt tctgcgtgaa accgaacctg aaatgcagga tattaatgct
     1861 tttcagatgc gctataacgc gttctttcag cctgccgagg gcgttcactg gttgcattga
     1921 aaccgtttcg tcttatcagc ctacagaccg cgcctgacct gtcgggtgga taagacgttc
     1981 acgccgcatc cggtaatctt tgccctgttc cgtttcgatc ttaactccag ctcaagcaac
     2041 accgtaaaag ttgcaaaaac gttaaattcg tcacacattt caatgttaaa gtggtcggct
     2101 tttcccctga aacatgccac gggtaacacc atgagtgaat ttattgccga aaaccgcggc
     2161 gcggatgcca tcacccgacc gaactggtca gccgttttct cggtggcgtt ttgtgtcgcc
     2221 tgtctgatta tcgttgagtt tttgcccgtc agtttgttga cgccaatggc ccaggattta
     2281 ggcatttcgg aaggggttgc cgggcaatcg gtgaccgtga ccgcctttgt ggcaatgttt
     2341 gccagtttgt ttattaccca gacaattcag gccactgacc gccgctacgt tgttattttg
     2401 tttgccgttt tgctgacgct ctcctgcttg ctggtttcct ttgctaactc attcagtttg
     2461 cttttaatcg gtcgtgcctg tctggggctg gcgctgggcg ggttctgggc gatgtcggcg
     2521 tcgctgacca tgcgtctggt accgccgcgt acggtgccga aggcgctgtc ggtgatcttc
     2581 ggcgcggttt ctattgcgct ggtgattgcc gcgccgttgg gcagtttttt aggcgagctt
     2641 atcggttggc gcaatgtctt taatgcggcg gcggtgatgg gcgtgctgtg tattttctgg
     2701 attatcaaat cattgccttc actgccaggc gaaccctcgc atcagaaaca aaatactttc
     2761 cgcttattac aacgtccggg tgtgatggca gggatgatcg ccatcttcat gtctttcgcc
     2821 gggcagtttg ctttcttcac gtatattcgc ccggtgtata tgaacctggc gggattcggc
     2881 gtggatggct taacgctggt gctgttgagt tttggtatcg ccagctttat tggtacgtcg
     2941 ctttcgtcgt tcattcttaa acgttcggta aaactggcct tagcaggcgc gccgttaata
     3001 ctggctgtga gtgcgttggt actgacgttg tggggaagcg ataaaatcgt tgctaccggc
     3061 gtggcgatta tctgggggct aacttttgca ttggttcccg tcggctggtc aacgtggatc
     3121 acccgctcgc tggccgatca ggcagaaaaa gccgggtcta ttcaggtggc ggttattcag
     3181 cttgctaata cctgtggcgc ggcaatcggc ggttatgcgc tggataatat tggtctgact
     3241 tcgccgttga tgttgtccgg cacattgatg ttgctgactg cattgttggt tactgcaaag
     3301 gtgaaaatga agaaatcctg atatcaggta aagaagcgtt ggtcagtagt tgttgctgtt
     3361 cttacatatc cgggcattct accttaccca acgcttccca ataattgctc tgattattac
     3421 gttccatcgc cacgatggcc tcgcgtatat gttgtttatt gttttcatct gacaacatta
     3481 acttttccca cgcttcatct gataatgacg catgtggcgt gcaaatttta cgtaaatctt
     3541 gtaataccgt ggttttctct tttgcggact gcagtgagct aaaaggcgac tcggcgtata
     3601 cgatgcctgg catagcgaaa aaagtgaaaa taagcagtag tgtcgttggt ttcatggatt
     3661 caacctttta caaatgtgta tcgaatgaag cataagtgta gtttgctttc atcgctctca
     3721 tcatgctgta cgaaacgcgc ttgcgtggtc agtaagaagt gccagacttt atattccact
     3781 tttattcctt tttattctta tcgatagcgt ttcgtttttt aaaccgcagc gaccttaccg
     3841 c
//
LOCUS       DAZSIO010000249         3824 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZSIO010000249 DAZSIO010000000
VERSION     DAZSIO010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.253"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(62..1420)
                     /gene="plaP"
                     /locus_tag="ACHX5T_003919"
     CDS             complement(62..1420)
                     /gene="plaP"
                     /locus_tag="ACHX5T_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR8468887.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLTINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1410..1472)
                     /gene="yoeI"
                     /locus_tag="ACHX5T_003920"
     CDS             complement(1410..1472)
                     /gene="yoeI"
                     /locus_tag="ACHX5T_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR8468888.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(1633..2616)
                     /locus_tag="ACHX5T_003921"
     CDS             complement(1633..2616)
                     /locus_tag="ACHX5T_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR8468889.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(2662..3486)
                     /locus_tag="ACHX5T_003922"
     CDS             complement(2662..3486)
                     /locus_tag="ACHX5T_003922"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR8468890.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3569..3823)
                     /gene="yoeB"
                     /locus_tag="ACHX5T_003923"
     CDS             complement(3569..3823)
                     /gene="yoeB"
                     /locus_tag="ACHX5T_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR8468891.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1019 a         1005 c          902 g          898 t
ORIGIN      
        1 aagaataaaa aaaccggagc caaatgttca ttcgactccg gtttattatt agaaaagatg
       61 attatgctac gtcttcgtac tgcggaaccg gattacggaa gctcttggtg acgcaagcca
      121 ggtaaatcag accgatagca gcccagatca gacccagaac cattgagctt tcttccaggt
      181 taacccacag cgcaccaacg gtcagcgcac cacacatcgg caggaacaga tactggaagt
      241 gatctttcag cgtcttgtta cgcttctcac ggatccagaa ctgcgagatg accgacaggt
      301 taacaaaggt gaacgccacc agcgcaccaa agttaatcag cgccgttgcc attaccaggt
      361 cgaagttgat tgtcagcagg gcaatcgcgc caaccaggat gatgttcatc gccggagtac
      421 gccatttcgg gtgtacataa ccgaagaagc ttttcgggaa cacgccgtca cgacccatta
      481 cgtacatcag acgcgctacg cctgcgtgcg ccgccatacc ggacgccagt acggtaatgg
      541 tggagaagat cagcgcgcca acctggaacg ctttacccgc aacgtacagc atgatttcag
      601 gctgtgacgc atccggatct ttaaagcgag agatatccgg gaagtacagc tgcaggaagt
      661 aagttgcaaa gatgaagatc atgccgccaa tcagcgcggt caggaaaatc gcacgcggga
      721 tcacgcgctc tgcatctttg gtttcttccg acagattgct gatgccgtca aagccggtaa
      781 aggagaagca caggattgtc gccccggtaa tcatcgggat aacatgcgca tcgccagacc
      841 agaacggacg cgtgctcgcc agcgtaccag cgccttcacc ttcaaatacg ccataaacaa
      901 ccatgcccag aatcaccgcg atcagcacta cctgcaacac gacgattaca gtgttgacgt
      961 tcgctacgga tttcagacta cgcaggttaa aggcggtcat aaaggccacc agcgccacca
     1021 caaacatcca cgatggaatg gaaggcacca gagcttcaaa atagattttc gccagcagaa
     1081 tgttgatcat cggcgcgaac agatagtcga gcagagaaga ccaacccacc ataaagccga
     1141 cagtcgggct aatggatttc tgggcgtaag tgtatgcaga gccagcagaa ggatagcggc
     1201 gaaccagctt cccgtagctc agagccgtaa acaggatcgc aatcaatgcg aacgcatagg
     1261 ctgtcggcac atgaccatcc gtgaggcctg aaacgatacc aaatgtatca aacagcgtca
     1321 tcggctgcat ataggctaga cccatcatta caaccggaac taacgtaagc gttttacgca
     1381 attccacgcg agaggtgttt ggagtaacgt tatgcgacat ggttattctc ctttacggtg
     1441 ataaccgtcg cgtaagcaaa aaattgcccc atttttttgg attcctcagc gacaacaact
     1501 gtcgattttt agtaaatatc tatccggtac gaagcccggc ctcttggtat gaatgattgg
     1561 tttgaagcaa aaaataaccg acgctaatta aacgtcggtt tttaatcatt ttttgacagc
     1621 ggcgcattgt gcctaaatgg gggggaaatg acaagagaat gagaggcttg tcataataat
     1681 ttttctttga atggctgatt ttccgtcatc agattttgca taaacaccgg cgatcgcact
     1741 atttgctaaa atttcatctc gccaccagcc gctggcaagc cctgcggtct gttcattcca
     1801 gccaatccag acaggttcaa agagagtttg ggcgaccact ttcttctcga ttaacgcgcc
     1861 actatcgaga aaacgttgcg ccagataccg tggtaaatag ccgcagccaa ggccgctaat
     1921 ttgcagctcc agtttggttt taaaatcgaa aacggtaatg gcttcttgtt cgtcgagaag
     1981 ttgtgaggcc gtagaagcgc ctgcctgcgc agtatccccc accacaatcg cccgatatcg
     2041 tttaatgata cgacggttta acggttcttc ttcctgggct aacggatgat gaggcgcgac
     2101 cgcaaagact tgttcaagat cgcccaaccg cgagaaacca aactcactgg aagatggtgg
     2161 ctcatgcatc gcgccaacga taatatccgc tcgcccctga gtcagggcat cccaggaacc
     2221 accgagtacg ccattgataa acttcaggcg cgtcacgcta tgatgttgat aaaaagcttc
     2281 aatgagaggc gcaagcagag aaaaaggaaa cgtgtcgtcc acgccaatca ccagctcgtt
     2341 ttcccagcct tcatgcagtt tgatcgcctg cttttccagt tctcgcacgg tatgcagaac
     2401 ttcccgccct ttctctaata acatttttcc ggtgcgggtg aatttagcgc ggtgtccgct
     2461 acgatcaagc aattgaatat ttaggtcgct ttccagcttg tgaacggtgt aactcagagc
     2521 ggacggcgtc ttgtagagtt tggctgacgc cgccgcaaaa ctgccctctt tttctatggc
     2581 atcgaggatc atcaacacat ccagcagtgg tttcatacac gcccccttgc agtgcatgcg
     2641 gcgtcctgct ggcgtgatcg tttactccaa cggcattacc agcggatcgg gatactggta
     2701 ttcaaatccc agttcattac aaatccgact gccatcaata atcttacctt tgccgctgtc
     2761 cagactattt ctgaactgcg gaggttgcag ccccagtaaa cgggccatct gcggatagaa
     2821 aacattacgc gcagggtgag ctggcgcaca tatattatag atgtgtccgc ctttaggtgc
     2881 ctgtaacaac agagtgatag cgccaataac atcttctaaa tggactaaat taacaccatg
     2941 ttcaccatca ggggcggttt ttccggcaaa gaagcgtccc ggatgacgtc ccggtccgac
     3001 caggcccgca agacgcagaa tatcgaccga agtaccgggt aaattgtgca gccagtcttc
     3061 gagttcttct aatactcgtc cactgttggt taccggatta cgcggggtag tttctttaac
     3121 cgtgccttgc gcgtcgccat agacagatgt tgagctggta aaaataattc ggggaatacg
     3181 atgggccagc gcgctatcca ctaactcttg taccgcttgt aaatagaact catcgccggg
     3241 gccgctacga cgtgccggaa gcgtaatgac cagcgcatcg gcatccataa gggcatccag
     3301 atcgtcagaa tcgcaaacta actcaggctc catgcgaagc agatagctat caatgccact
     3361 cattcgggcc gcttcgacac catcttgtgt ggttttactc ccggtgactt gccagcctcg
     3421 cgctgaaagt gacatcgcca gcggcatacc taaccaccct aacccgacaa ttgcgacctt
     3481 tttcatccgt tatctcctga tacaccttag atctataagg ctacgctagc gtatcaaaac
     3541 tgacaattca ttctatgaat gaatctgttc aataatgata acgacatgct gcaatgagca
     3601 gtgaatcatc agtaaccgcg tataccagac ggtgctcctc tgtaatgcgt cgggaccaga
     3661 aacctgacaa attatgtttc aggggttctg gtttcccctt accttcaaat ggtgttctgc
     3721 gggtatcttt gataagttca ttgatctttt taacaattcg cttatctgtt tcctgccagt
     3781 acagataatc atcccatgat tcctcagacc agattagttt cact
//
LOCUS       DAZSIO010000250         3824 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZSIO010000250 DAZSIO010000000
VERSION     DAZSIO010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.254"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            50..325
                     /gene="ypdI"
                     /locus_tag="ACHX5T_003924"
     CDS             50..325
                     /gene="ypdI"
                     /locus_tag="ACHX5T_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFR8468892.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(402..644)
                     /locus_tag="ACHX5T_003925"
     CDS             complement(402..644)
                     /locus_tag="ACHX5T_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR8468893.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            997..1917
                     /gene="lpxP"
                     /locus_tag="ACHX5T_003926"
     CDS             997..1917
                     /gene="lpxP"
                     /locus_tag="ACHX5T_003926"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR8468894.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2272..2343
                     /gene="ypdK"
                     /locus_tag="ACHX5T_003927"
     CDS             2272..2343
                     /gene="ypdK"
                     /locus_tag="ACHX5T_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR8468895.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2408..3646)
                     /gene="alaC"
                     /locus_tag="ACHX5T_003928"
     CDS             complement(2408..3646)
                     /gene="alaC"
                     /locus_tag="ACHX5T_003928"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR8468896.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
BASE COUNT          995 a          969 c          887 g          973 t
ORIGIN      
        1 gccataacat cgtatggttc tcagtgaacc attttcagga taactttcta tgaaagtaaa
       61 cttaatactt ttcagcttat ttttattggt ctctattatg gcatgcaatg tttttgcatt
      121 ttccatttct ggtggtgtaa gtgagaggag ctataaagag actgaaaaaa catcagcgat
      181 gacgaccaca cactctacaa aacttcagcc atcacaggcg attttgttta agatgagaga
      241 agatgtgcca ccattaaacc tcacagaaga aatcacgcct acttatccga caaaggcgaa
      301 ttatcttatt catcctgtgc gatagcgtct gtcacatggc aggtgaacaa tcatgtctga
      361 tgctgatgtg aacacctgcc caaatgagcc atcctgaccc actatcgcct gccatacccc
      421 accataaagc gaatcatcaa gccacaagcc ggggcaagaa acataaatat ccccgtcacc
      481 agttcattac cgccagttaa ccacatggtg aagtaataaa tgagtgcagc gaggatcacc
      541 atgccgaaga aagaagagac cgccgatacc acgttcagta cctgaccgat gtagccgctt
      601 acgcagacaa tacacagggc gagaaacatc cataaataaa tcatagctca acctcctgcc
      661 gcgctaaaaa acctgtaact tcagtcactt taatagtaag acaatcctta acctctggca
      721 atttttgcta agcatcaatg aaaacagcac gttaaatgca acgctattgc gtgcggaaaa
      781 ttttttactt tgcacgatta ataatcagta gctgaaagca gtcagccagt gataaagtag
      841 agccaccgca ttcatgctcc tcgtggttat gtcctgacta gtctttacac tctttacagg
      901 aaccattgtc gtacatgatg gcccaaccaa ttgagtgtac cgctgtcgac atggaatctt
      961 ctgcgataca acaattcgta tctacagaag gtaactatgt ttccacaatg caaattttcc
     1021 cgcgagtttc tacatcctcg ctactggctc acatggtttg ggcttggtgt actctggctt
     1081 tgggtacagc ttccttatcc tgttctctgc tttctcggca cgcgtattgg cgcaatggcg
     1141 cgaccattcc tgaaacgtcg tgaatctatc gcccgtaaaa acctggaact ttgtttcccg
     1201 cagcattctg cggaagaacg cgagaagatg attgccgaaa actttcgttc actcggcatg
     1261 gcgctggtag aaaccggcat ggcatggttc tggcccgaca gtcgcgtacg taaatggttt
     1321 gatgttgaag ggttggataa ccttaaacgc gcacaaatgc aaaatcgcgg cgtaatggtt
     1381 gtcggcgtcc attttatgtc gctggaactg ggcggccgcg tgatgggact gtgccaacca
     1441 atgatggcta cctatcgtcc acataataat cagctgatgg aatgggtgca gacccgtggg
     1501 cgcatgcgct ctaacaaagc gatgatcggc agaaataatc tgcgcggcat tgtcggtgca
     1561 ctgaagaaag gtgaagcggt atggtttgct cccgatcagg attatggtcg taaaggcagc
     1621 tccttcgcgc cgttctttgc ggtggaaaat gtcgccacaa ccaatggcac ctatgttctc
     1681 tcccgtctct ctggcgcagc catgttgacc gtaacgatgg taagaaaagc ggattacagc
     1741 ggatatcgtt tgttcatcac cccagagatg gaaggctacc cgacagatga aaatcaagcc
     1801 gctgcttata tgaacaagat tatcgagaaa gagatcatgc gcgcaccgga gcagtacctc
     1861 tggatccacc gtcgctttaa aacgcgcccg gtgggagaat cgtcgttgta catttaacaa
     1921 actgctctta ctctttccct cctcaaatcg gccataataa cctcatgatt tttatagctt
     1981 ttttcttaag ttttttcctt cgctgaatat ctaattatta ccgtcagaaa ttaaactgtc
     2041 gccgatatga gacacttgta agttactaaa agtaaataag aaaatgcctc ttgtctcgtt
     2101 tcagttttag gcgtacatta gcgccgtctc gagcagttta gctcagaatt ctggggtgat
     2161 gcccacggta aaatacaggg cttttaaggt cgctttgccc ggtgtcaact cactgtatca
     2221 ggtgtaatga agtcattcag gcgtaacagt aattacgcgg agagatgtaa agtgaaatat
     2281 ttctttatgg gcatttcttt tatggtcatc gtttgggccg gtacttttgc cctgatgatc
     2341 taaaagcaaa ctgtgcaatg aaaaacagga gccatcaggc tcctgtttgc atttctgacg
     2401 acggtgttta ttccgcgttt tcgtgaatat gtttgctgct gacgggtagt aaaccgtcgg
     2461 cacggaacat cgctttaatc cctctgatgg cctgacgaat acggtcgtgg ttttcaatca
     2521 gtgcaaagcg aacatgcgta tcaccatagt cgccaaagcc aatccccggc gagacacaaa
     2581 ccttcgcttc gttaaggagc ttcttggcaa attccagcga tcccatggcc gcatatggtt
     2641 ccgggatttt cgcccagaca tacatcgaag ccttcggcat ttcgaccatc cagcccgctt
     2701 catgcagccc tttaaccaat acatcacgac ggcgtttgta ctgttcggca atgtcgcgca
     2761 cgcactgttg atcgccctcg agtgccgcaa tcgccgccac ctgcaacggc gtaaaagtgc
     2821 cgtaatcgtg atagcttttg attcgcgcca gggcactgac cagcgttttg ttgccgacca
     2881 taaagccgat acgccagccc gccatgttgt agcttttcga cagcgtaaag aactcgaccg
     2941 ccacatcgcg tgcacccggc acctgcataa ttgacggcgc tttccagcca tcgtagacga
     3001 tatcggcata ggccaggtca tggaccacca gcacatcgta gcatttcgcc agcgccacaa
     3061 ccttttcgaa gaactccagt tccacgcatt gcgcggttgg gttcgacggg aagccgagga
     3121 tcatcatctt cggtttcgga taactttcac gaatggcgcg ctccagttcg ttgaagaaat
     3181 cgacaccttc caccagcggc actgagcgta cctgcgcccc ggcaatcacc gcgccataaa
     3241 tatggatcgg gtaacttgga ttcggcacca gcaccgtgtc accatgatcc agcgtcgcca
     3301 gcatcagatg cgccaggccc tctttcgaac caatagtgac gatggcttct gattccgggt
     3361 cgatttcaac gtcgtagcga tcctgatacc agcgggaaat ggcgcgacgt aaccgcggaa
     3421 tgccgcgtga agtggagtaa ccatgcgtgt ccgggcgctg ggccacagta cataattttt
     3481 cgacgatatg cggcggagtc gcaccgtccg ggttacccat gctgaaatcg ataatatctt
     3541 cgccgcgccg acgcgcagcc attttcagtt cagcggtgat gttaaaaacg tagggcggga
     3601 gacgatcaat gcgcgtaaag cgacgttcag ggcgagtgtc agccataatt tcctcagatt
     3661 aacgttagcg cccggaccgt ccgagcgacg ctgccacaat ggtggctgtt ttgaaaatag
     3721 cctgattaat ttctgtctgt ccagcgtttt agaacatttt tatcaatgga tttaaatgag
     3781 gaactccact tcaaaaacag gaaatttttc tacgctaaca agaa
//
LOCUS       DAZSIO010000251         3815 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZSIO010000251 DAZSIO010000000
VERSION     DAZSIO010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.255"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..77
                     /locus_tag="ACHX5T_003929"
     CDS             <1..77
                     /locus_tag="ACHX5T_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468897.1"
                     /translation="AFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(91..282)
                     /locus_tag="ACHX5T_003930"
                     /pseudo
     CDS             complement(91..282)
                     /locus_tag="ACHX5T_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            280..604
                     /locus_tag="ACHX5T_003931"
                     /pseudo
     CDS             280..604
                     /locus_tag="ACHX5T_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(634..3546)
                     /locus_tag="ACHX5T_003932"
     CDS             complement(634..3546)
                     /locus_tag="ACHX5T_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFR8468898.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            complement(3603..>3815)
                     /locus_tag="ACHX5T_003933"
     CDS             complement(3603..>3815)
                     /locus_tag="ACHX5T_003933"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468899.1"
                     /translation="GYLNRGDMFTSEQHRYSNEKKEFQRRVYLRGDAFINLYTNFYSC
                     IMTGLQLLRHFQRIALPAYHPTFFIP"
BASE COUNT          955 a         1022 c          947 g          891 t
ORIGIN      
        1 gggccttccc gagagtggta aaggagaggc cctggaaata ccccacagcc ccgaaaaaga
       61 gccagtcagt tgcttaactg actggcatta aggccgccgg ggacgttttt ttaccaccgg
      121 gagtttgggc ataacggtgc tttcagtaag tacgacaggt actgtggagg cacttccgta
      181 aagaagggca tcaagctcct gctccgacat acctgcaggc aaaggccatg aaagccctga
      241 tttccggaag cgagcgaaca gtccacaaac tgttgaacgt ggtgtcagcg gcggacaggg
      301 ataagccctc gtcagcagtt ccgtcagcac tgcgacagcg ttctttaccc ggtcaaaaca
      361 gcgttgcggt gcaccacgcc tgacggatga actgcgtgct cagggttacc actttaacgt
      421 aaaaaccgtg gcggcaagcc tgcgccgtca ggggctgagg gcaaaggcct cccggaagtt
      481 cagcccggtc agttaccgcg cacatggcct gcccgtgtca gaaaatctgc tggagcagga
      541 tttttacgcc agtggtccga accagaagtg ggcgggagac atcacgtact tacgtacaga
      601 tgaagttcgg ttacatcctg tcagcacgga acctcacgct ttttgagcgg cacgacggat
      661 cccccccacc cagtatccga cagacttttc actgcgttcg taaaaaccct tcttccctcc
      721 cagcatatcc actaattccg gtgacatatc ctgcagataa gtctgcaaat catgccccag
      781 atcttccggt gactgcaact cataaaccgc gcgccacaca tcacccttcc ttattttaag
      841 ccccgggtta agaatatact gtccgtggtt cagcctcaga aaaagctttt ttccataagg
      901 atttttgctg ttaacttcat gttgagagag ctggctgctg atataactcc ttcgtttgcg
      961 gcgttccggc agcaacgctt caggaagatg aaccacacac tctgccagtc gtgccgcact
     1021 gaaggcttcc gaaaaaaaca gagcacttcc cagatgcgtg tggaacagcg cctgcatcag
     1081 gatcaccaga aacattgcca tctgccggtt gtccagtttg atgaggcgtc cttccacctg
     1141 cagtgacaga ctttcaggtt tcagcagggt atacaactgc gcacttttct gttgcgcata
     1201 acggggcgta ctgaccgccg ctgacagaac ttgatgataa gccgccagcc cgtcattacc
     1261 ggtcagcaag ggatctgcac cacgctccag cagtaactga accagtgcaa tattacctgc
     1321 ccgggctgct gacatcagcg gagtaagatt aaacggcgaa cggtgatcaa caccataacg
     1381 ttcaatatcc cgcaacacgg caaccgggtt attcattgag tatggccgga aatacttcag
     1441 ctccagtaac tgcagagtct tcgttctggg ctgccttgtg ggcttaaaat cttcccggta
     1501 cagtgcatta tacagcggga acagggaata aatcagactg tactccagcg cctgcagttg
     1561 tatggtttta tcggctgtac ctgcgaggat atgctgtttc cactgtcgtg catcctctgc
     1621 ggtgataatc tgccatggca tctcagatgt ctgcaggatc cggctacgga tggtgtccgc
     1681 ctgctcctgc ttcccctgtt tttcaagcag atgtgcggtt ttctgccact catcacggct
     1741 ggaaatctct tcctgcagat tcagaggctc ttcctgatgc gtgagcgcaa gcagactcca
     1801 cagaggatgc tccacctgct gttcaacaag atacacattg tgtttagcac gggtgagcgc
     1861 aacatacagc gcgttggtaa aaaatttata gatttccgct gaacgatcct gcctgtcccg
     1921 cggacgggaa taacgggcct cgccctcaag atcagccggt gtaagaccct cgcaaatatc
     1981 atcaaatgcc tgccgggcag cgcttactat attgtaaagg atcaccgttt cgtattccag
     2041 ccccttaacc tcctgaaccg aaaagaccag cggcgtactg aaccagcatc gcgcctgtgc
     2101 tttctgttcc ggatgcatca caatgaccgc tacacgattc gaaagtgagg ttttggcatt
     2161 cagttcctgc tttgtctctt ccctatcgtc cagcaaccgg atggtccctt ctgcctgacc
     2221 gcagcttctg acaaaatggt gactttcccg atcaatagca ctaaagcgga cctgtttgag
     2281 tctcagcaac cggttagcca gtgcagtgac gtggtgcccg ttacggtaat tcgcctgcag
     2341 gatattaacg gttgtgtcgt ttccctgttg ctgccggaaa aagagacttt tgagcgaact
     2401 ccatgagaaa aagctcggat gaactatctg attggcatca ccacagagta aaaactgacc
     2461 gggatgacgt agcgttttca gtaccagttg cagttgaaca ggcgtcatat cctgaacttc
     2521 atcaacaaaa atgacatcat aacggggagt tgccaacgaa agatactgat gactgagcag
     2581 attactgtca tactgatgcg actgtttcag ccatgcgata taacgttcaa acaggacata
     2641 gaccgtcggg cggtcctcca tgccatagag cgactgccgt atccccagtg acagatacgc
     2701 ctcccgggac agcggcccat tcccggaggc aaccgcaccg atcacgcccc tgaactcctc
     2761 atacagggta tgggcagccc ccagtgcggc gatagccctg ttgcggggca gccagtcgct
     2821 gaacgcactg aaagtcacct ccctgccttc cggaatgcgc agagtttcca gaaattccgt
     2881 cagcgaaaga aagtcaatat tctgtacctc actcccttcc cctgaagcgt catagagagt
     2941 acgcgctttt tccaccagaa atgaggaaag gctgagatac agtatatcac cagcagcctg
     3001 cttcattttt tccagtacca gcgccgtctt accacttccc gccgagccaa caatcaccag
     3061 cggtagcggg tgttcgtaca gtgcctgctg atcatcatca aaggacagca ttttatccag
     3121 gcggtggaaa cgcccatgag acggattgat atacgtcagc gcttcagtcg cgatatcaac
     3181 aggatccggc actggttgtt gttgtaagcg cccttcatca atcaccacgt ttctgcgaag
     3241 aaaacgtgaa gtgttatagg catgattgcg aatatattcc agaacaaggc agatagtttc
     3301 accgtggtag cgatacagcg aaaacagtaa tcggtcccgg atactgagcc gggcgcggta
     3361 aagattggtg tcaattttac gaacatcagc ctgaacaaag ttatcggtgg caatagcctg
     3421 tgcaaatttt ttataacccg ggatcgtgtc aggattcaga gtatgataaa gacagatttt
     3481 catggctgcc ggtttccgtc gtgcaaaaaa catgctgatt ttacgaagga atgccttact
     3541 gaacaacctt gcccggaata ccccccgaaa tcagatggaa caatcagaaa aatgctctgc
     3601 agttatggaa taaagaaggt ggggtggtac gccgggaggg cgatccgctg gaaatggcgc
     3661 agtagctgca ggcctgtcat gatgcaggaa tagaagttcg tgtacaggtt aatgaaagcg
     3721 tcaccccgta aataaactct gcgctgaaat tccttttttt cattggagta cctgtgttgt
     3781 tctgaggtga acatatcacc tctgttcagg tagcc
//
LOCUS       DAZSIO010000252         3807 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZSIO010000252 DAZSIO010000000
VERSION     DAZSIO010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.256"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            437..2090
                     /gene="ushA"
                     /locus_tag="ACHX5T_003934"
                     /pseudo
     CDS             437..2090
                     /gene="ushA"
                     /locus_tag="ACHX5T_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar hydrolase/5'-nucleotidase
                     UshA"
     gene            complement(2101..2271)
                     /locus_tag="ACHX5T_003935"
                     /pseudo
     CDS             complement(2101..2271)
                     /locus_tag="ACHX5T_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2404..2523)
                     /locus_tag="ACHX5T_003936"
                     /pseudo
     CDS             complement(2404..2523)
                     /locus_tag="ACHX5T_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2597..3596)
                     /locus_tag="ACHX5T_003937"
                     /pseudo
     CDS             complement(2597..3596)
                     /locus_tag="ACHX5T_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1053 a          878 c          874 g         1002 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tcataattgg ttatgatgca atggccgtcg
      781 gtaatcatga atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat
      841 ttcctttcct ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat
      901 ggaaaatttt catccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata
      961 ctgcgaaaac aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg
     1021 aggcgaggag tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg
     1081 caacacatat gggtcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg
     1141 aaatggctcg cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag
     1201 ctcctgtctg catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct
     1261 gtgtaccaga taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg
     1321 tcggtcaggc tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac
     1381 acccagttaa tctcaaaatg agaattacca gagaagatgg aaaaacggaa ttttcttttt
     1441 acacccccga aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata
     1501 aaggcaaagc acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca
     1561 gaagtaaagt tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag
     1621 aacgtattga cgcagatttt gctgttgtca gtggtgggga aattcgagat tccatagaga
     1681 gtggtaacat cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa
     1741 gtattgattt gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac
     1801 cggattccgg agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa
     1861 tcgaaatgct aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa
     1921 catttagctt taatgctact ggtggggatg gctatccacg tattgataat agacccggat
     1981 acataaatac cggatttatt gatgctgaag tacttatcga atatatccgg aaacattcac
     2041 cgctggatgc tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt
     2101 gacgatcatc acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg
     2161 tatgtttgcg atatgggaat gcagttgctg tattttgtgt ttctgcttct gccagttgtt
     2221 gctgaattta accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt
     2281 cgctgattaa agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccatat
     2341 tgatatttac taagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc
     2401 cggtcagtcc tggatatgct caatgacgta acgcagccat ttttctggct ccacattgtt
     2461 cagacggcat gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctatcgga
     2521 accccgccgg agagagtttc actctccggc tgctcacgga accgtacgtg acactctcgc
     2581 gtcatacggc tcttgatcac tcattcgatg cacgtagtaa ataccagtgg gcaaatagct
     2641 tcggatactg tttccttacc agcgccagct ttcgctgagc cttccggttc gaaagtctgt
     2701 atttagactg catccactta agcaaacgtg actgcattgc actccacagt cgatagctga
     2761 agtttctgga ccagaacttt ccatagtact caatccagcc tctcactatc gcattgtagc
     2821 gcctcgcaaa atccagcaaa ctctcagctg ttgagcgatg tatacgccac ttcttgattg
     2881 tttctgttat tttgcacatt gctgcatttg atgcacccgg catacatttt ctgtacaatt
     2941 cgcctttgaa attcttcaga gtacgcactt tgaagtcata tccgagagtg gcatacaaca
     3001 tcatcggcgt acctttcgaa gggaacgccg ggaaactgac gttgcatcca catatcaaag
     3061 gcatagtgca ggaacagatt tgagagcagg ggggagatta cgccgccctg cggcgttcct
     3121 cgaccccgtg ccagcattgc tccatccggc atttgcaccg gtgccttcag cctgcgctca
     3181 atgtacatca ccacccagcg tgcctgagta tgatgctgca ctgtcttcag tagcaggtca
     3241 tgatcgatat catcaacgaa gcctttaata tcgacttcca ccacccagtc aaacttccag
     3301 cagcgtttcc tcgcctgcgc aatcgcctgg tgtgcggatt tacccggtcg ataaccatac
     3361 gagtctgcat ggaacagggg ttccagtacc ggctcgagta tctgcttaac gaccatctgg
     3421 gcgatccggt ccgacacggc cggaataccg agcggacgta caccaccgtc agctttggga
     3481 atgtccacac gccttaccgc ctgcggcata taactgccgg aagccattct gttccacagt
     3541 ttgtagagat tgtctgtaac attctcgtca aacccggcca gtgtctgccc gtcaatgcct
     3601 gccgccccac ggttggcttt tactcgttta taagcttccc atacctgtcg tttacttaca
     3661 ggaaatggct ttgccgattt tacagcagtc ctcccatcgg cattgtcgcc cgtggttgcc
     3721 gccgcaccat cagcacaatg gggcaagccc tttgctccgg acccattacg gtccttcacc
     3781 gctactacgg tttactccgt ccctgta
//
LOCUS       DAZSIO010000253         3803 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZSIO010000253 DAZSIO010000000
VERSION     DAZSIO010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.257"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            21..1079
                     /gene="tsuA"
                     /locus_tag="ACHX5T_003938"
     CDS             21..1079
                     /gene="tsuA"
                     /locus_tag="ACHX5T_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR8468900.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            1093..1320
                     /gene="tsuB"
                     /locus_tag="ACHX5T_003939"
     CDS             1093..1320
                     /gene="tsuB"
                     /locus_tag="ACHX5T_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR8468901.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1363..2787)
                     /gene="sbcB"
                     /locus_tag="ACHX5T_003940"
     CDS             complement(1363..2787)
                     /gene="sbcB"
                     /locus_tag="ACHX5T_003940"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR8468902.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            2969..>3803
                     /locus_tag="ACHX5T_003941"
     CDS             2969..>3803
                     /locus_tag="ACHX5T_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8468903.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     EQ"
BASE COUNT          906 a          891 c          995 g         1011 t
ORIGIN      
        1 ttcatctggt taattaattt atgttttcaa tgatattaag cgggctaatt tgtggtgctc
       61 tgctgggatt tgtcatgcag cgtgggcgtt tttgcctgac tggtggtttt cgcgatatgt
      121 atatcgcgaa aaataatcgc atgttttacg ccttgctgat tgcaatttcg gcacaaagcg
      181 tgggggtttt tgcgttaatt caggcgggcc tactgactta cgaagccggg gcgttcccgt
      241 ggcttggtac tgttataggt gggtatcttt tcgggctggg aattgttctg gcgggtggat
      301 gtgccaccgg gacctggtat cgcgcgggtg agggattgat cggcagttgg atcgcgcttt
      361 tcacttatat ggtgatgagt gcggtgatgc gttctccaca cgccagtggt ttaaatcaaa
      421 ccttgcagca ctacactact gaacataact ctattgctga gacttttaat ttgtctgtgt
      481 ggccgttggt taccgttttg ctggtgataa cgctctgggt ggtgatgaaa gagttgaaga
      541 agccaaaact caaagtcgcg accttaccgc cgcgccgaac cgggatcgct catattctgt
      601 ttgaaaaacg ctggcatccc tttgtcactg ctgtactcat cggtttgatt gcgcttttag
      661 cctggcccct gagtgaagca accggacgca tgtttgggtt aggagtcact tccccaacgg
      721 ccaatattct tcaatttctg gtcgcgggtg acgtgaaata cattaactgg ggcgttttcc
      781 tggtgttagg gatcttcgtg gaggcattta ttgcagccaa agcgagccgt gagttccgcg
      841 ttcgcgcagc tgatgcacaa acaacattac gtagcgggtt aggtggtgta ctgatgggct
      901 tcggtgccag tattgcaggt ggttgctcta tcggtaatgg actggttatg actgcaatga
      961 tgacctggca gggctggatt ggccttgtat ttatgattct cggagtctgg actgcgtcct
     1021 ggcttgtgta tgttcgaccg cagcgtaagg cgcgactggc aactgctgcg gcaaattaag
     1081 taaggtgtaa agatggcgat taaaaagctg gatgtggtga cgcaggtctg tccgtttcca
     1141 ctcatagaag caaaagcagc actggcagaa atggccagcg gcgatgagtt ggtaattgag
     1201 tttgattgca cccaggcaac tgaagcaatc ccacagtggg cggcagaaga aggacatgcc
     1261 atcaccgatt atcagcagat tggcgatgca gcgtggagca tcaccgttca aaaagcctga
     1321 ctcaacattg tcctccgccg taccagcggc ggcggcttca aattagacga tctcttccgc
     1381 gtactgccaa agtgctttta acagcgccac tttctctttg tcatcggcat attgttgtac
     1441 cagcatctgc aattcatcag cataaccctg caaaaactct ggcgtgaaga cctggtggcg
     1501 gtgctccagc cagcgttgct gctcggcata atccagcgtc cccgggaagt tgcgtgcccg
     1561 ataattgaac aacaactttt caattcgttt atcaacaaaa gtgatatcca gtgccggtaa
     1621 attacgcggc tcggtttcca gcacaatttt cattgctgca cgatcggcgt cactgaaaaa
     1681 gccattataa agctgtgcat ccacattatc tgaaggtgta aacggttcgg cttccgcgaa
     1741 tatcgctacc actttttcac gcacttgcgg attttcacgc agaattttta ggttatcgag
     1801 gcaatgctga cgattaattc ccagtcggtc ggcatcttcc gggcgtagcg tattcgcctg
     1861 ggccagtacc ggacatttat tgatatgcac cagcttaacc ggaacggcgg cgttatcgcc
     1921 aagatcggct tttgcggtat ataaacgctc gcgcaatgtg tcgatatcca gctccagtaa
     1981 tggcgaaatg tctcctgcca aatccgccat aattacggca ttgcgatttt caggatgcca
     2041 cgccagcggt gccacccagc tggtattgcc acgccatgcc ccaaacattc cggaaacgtg
     2101 agccaggggt ttcatctgcg gaacatcaat caacgccatc agtttgtgtt tattacgatg
     2161 ggtaaagaga taatcaaaca ggcgtggctg acgcgttttt accagtttcg ccatcgcaat
     2221 agtggcgtac acatcagcca tcgcatcgtg ggcgttgcta tgttcaatac cattcgcttt
     2281 ggttaaatgc tcaaggcgaa agctcggtag accgtcatca ttttcaggcc agtttattcc
     2341 ttccgggcgc agggcataac aggcacgcat aacatccagt aaatcccagc gcgagttatc
     2401 atgctgccag ctccaggcgt aaggatcgta gaaattacga taaaaaacgt tgcgtgtgac
     2461 ttcgtcgtcg aaacgcacat tgttgtagcc cagaatacag gtcttcggta cggtaaaaag
     2521 cgagtgaata cgggcggcaa acgcggcttc gttttctcct ttcgcccgtg cctcctgcgg
     2581 ggtaataccg gtaattaata ctgctccagg ccggggtaaa tagtcatccg cgggcttgca
     2641 gtaaaagact tcgggttcgc cgatgacatt gaattcgtca tcggtgcgaa tggctgcgaa
     2701 ctgtgcaggg cgatctaacg cggggtgcgt gccaaaggtt tcgtaatcgt gaaagaggaa
     2761 ggtaggttgt ttatctgtgt ctgtcattgt ctagcattgt ccactgtagt taattaactg
     2821 tctgttataa ctcgaaaagc aacccttttt tcacctgagt atgtctacga acgtcaaata
     2881 tagttgttta ctgtgcgctg ttgtccatgt gatattgtgt accagatgtg tgccaaacta
     2941 ttatttctga gcgtaccaaa tattatttat ggctataagc gacacaaaac tgcgtactat
     3001 ttatggtaaa ccatattcgg gcccacaaga agtggctgat gccgatggcc tcagcgtacg
     3061 aatttcaccg aagggggtca tccagttcca gtaccgctat cgctggcatg gcaagcctaa
     3121 tcgacttggg cttggtcgat acccatccct gtctttgaag gatgccagac agatcactgc
     3181 tgacttgcga aagctctatt tctcaggaac ggatccacgc acctattttg aagagaaggt
     3241 ggagaactcc atgacggtcg cccagtgtct cgactactgg ttcgacaact acgtctctac
     3301 aactctcaga gaaaagaccc aggcacttta tcgatcaacg gttatgaagc gcatgcatga
     3361 cgccttccct aatcgtccgg catcttctat cacggttaag caatgggttg acctgcttac
     3421 cgaagaagaa aaagataatc cacgccgagc aaggcaggtg ctaagtcaac taagatcagc
     3481 aattagttgg tgcatgcggc gtcagttgat agatagttgc gcaattatga gcatccagcc
     3541 aagggacttc ggctcccgcg ctgaggtagg ggatcgggta ctgtcgtatc acgaactggc
     3601 taagatttgg cttgctattg aaagaagccg tgcgtctacg tcaaataagc tccttcatca
     3661 gatgcttatg ctgtgggggg cgaggctctc agagcttagg ctggcaacaa agacagaatt
     3721 tgacctgctg gacaacgtat ggaccgtacc gaaagagcat agcaagatgg gtaatgttat
     3781 ccgccgtcca atcttcgaac aaa
//
LOCUS       DAZSIO010000254         3738 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZSIO010000254 DAZSIO010000000
VERSION     DAZSIO010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.258"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            108..2105
                     /locus_tag="ACHX5T_003942"
     CDS             108..2105
                     /locus_tag="ACHX5T_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFR8468904.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2168..3445
                     /locus_tag="ACHX5T_003943"
     CDS             2168..3445
                     /locus_tag="ACHX5T_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HFR8468905.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
BASE COUNT          904 a          852 c          941 g         1041 t
ORIGIN      
        1 ttccaccatg cattacgatt aacgccgcat agccagttga actttgctac tttgtgagag
       61 gtagtacctt ctatccagtg cgaatttaat taatggaata aatgattatg agtgaaaatg
      121 atacaatccc aaagaagtct acaagtcaga ttaacaaagc ggtattcttt acatctgctt
      181 tgctaatttt ccttcttgtc gcctttgccg ccgtattccc ggatgtcgcc gacaaaaatt
      241 ttaaactact tcagcaacaa atcttcacga atgccagctg gttctacatc cttgctgtgg
      301 ccctgatttt actgagtgtc acgttccttg gactctcacg ctacggtgat atcaagctgg
      361 gcccggacca tgcgcagcct gatttcagct accactcctg gtttgcgatg cttttttcgg
      421 cagggatggg gatcggcctg atgttctttg gcgttgccga acctgtaatg cattatcttt
      481 cgccacccgt tggcactcca gaaaccgttg cggcagctaa ggaagcaatg cgtctgacct
      541 ttttccactg gggactgcac gcatgggcaa tttatgccat tgtggcgctg attctggcgt
      601 tcttcagtta ccgtcacggt ctgcctttaa ctctgcgctc cgcactctat cccattattg
      661 gcgatcgcat atacggacct gtaggacatg cggttgatat tttcgctgtt ataggcacgg
      721 tctttggcgt tgcgacatca ctgggttacg gtgttttgca ggtgaatgcc ggtttgaacc
      781 atcttttcgg ggtgcccatc aatgaaacgg tgcaggtaat tctgatcgtg gtcatcacgg
      841 ggttagcgac gatttcagtg gtgtccggtc tggataaggg aatacgtatc ctgtctgaac
      901 tcaatctggg tctggctttg ttgctcctgg cgctggtcct gtgtctggga ccaaccgtgc
      961 ttctgctgaa gtcatttgtg gaaaatacgg gcggttatct ttcggaactg gtgagtaaaa
     1021 cgttcaacct ttacgcgtat gagcccaagt cgagcaactg gctggggggc tggacattac
     1081 tgtactgggg atggtggctt tcatggtcgc cgtttgtggg gatgttcatc gcacgggtct
     1141 cccgcgggcg aaccattcgc gagtttgtca ccggcgtgct gtttgttccc gcgggtttta
     1201 cgctaatgtg gatgacggtg tttggtaaca gcgcgatcta tctcattatg aaccaggggg
     1261 ccacagacct cgccaatact gttcagcagg atgtggcgct ggccctgttt aatttcctgg
     1321 agcatttccc gttctcttct gtgctgtcat tcattgcaat ggcgatggtc atcgtcttct
     1381 ttgtaacgtc tgctgattcg ggggcaatgg ttgtggatac tctggcatca ggtggagtgg
     1441 caaacacacc cgtctggcag cgaatattct gggcctcgct catgggcatt gttgcaattg
     1501 cgcttctcct tgccggaggg ctaagtgcgc tgcaaacggt gacaatagcg agtgcattgc
     1561 ccttctcagt gatcttacta atatctatat acggactttt aaaagctttg cgccgggatt
     1621 tgaccaagcg tgaaagcctg agcatggcga caattgctcc tacggctgca cgtaacccaa
     1681 ttccttggca gagaaggtta cgcaatatcg cgtatctgcc gaagcgatct cttgtgaaac
     1741 gttttatgga cgacgttatc cagcctgcca tgacgctggt tcaggaggaa ctgaacaagc
     1801 aggggacgat aagccacatt agtgatgcag tcgacgatcg tattcgtctt gaagtcgatt
     1861 tgggcaacga gctgaatttt atatatgaag tgaggcttcg cgggtatatc tcaccgacct
     1921 tcgcgctcgc cgcaatggat aatgatgagc agcagactga acaacatcga tattatcgcg
     1981 ctgaggttta tctcaaagaa ggcggtcaaa attatgatgt gatgggctgg aaccaggaac
     2041 agctgattaa tgacatactg gaccagtacg aaaaacacct gcacttcctg cacctggttc
     2101 gttaatagca acatgccgtc ctgggggcgg caattattat cccggccgca atatgaggga
     2161 atgcagaatg atttcacgct ggaaatggat gctgaagcag acaattaaaa aactatggtt
     2221 cagggcaacg ttattcgcaa ttgtcgcgat aataacggcc cttttatcaa ttctttttaa
     2281 atcaatgata cctgagtcgg tttccgtgaa ggttggtgcg gaagcagtcg ataacattct
     2341 gaacatactg gcatcgagta tgctggcagt gaccacattt tcgctgagta tcatggtcac
     2401 agcctacggt tcagccacta ctaatgtgac tcccagagct acgcgtttag ttgttgaaga
     2461 cgtcaccaca caaaatgtac tggccacctt catcggttct tttctcttca gtctggtagg
     2521 gattattgcc ctcaatatgg gagcttatgg agaaaggggg agagtcattt tattcattgt
     2581 cacactggtt gtcattgcct taatcctcat cacattgctt cgctggatac agcatttgac
     2641 ctctctgggg agggttggtg agacaacggc acaagtagaa caggcggcca tcgaaacatt
     2701 tattgcgaga gcaagaaatc cctgtctcgg tggatatcca tggcttgaga acaatgaaca
     2761 gccgaaagga acggttgcag tttatccgaa gaagattggc tatgttgaat atattgatat
     2821 ggtgaaactt agcaagctgc tgaccaatga tccccgtcat gtatacctcg tggcgcagcc
     2881 aggcagtttc atacatccgt ccatgccagt tttgtacctg agtcagggcc aggagtcgtc
     2941 aatcaacacc gatttacttg agacgattat tgtctcggat gcacgttcat ttgctcagga
     3001 tcctcgattt tgtcttagcg tcatggccga aatagcctgc cgggcccttt cccctgcagt
     3061 gaacgatcct ggaaccgcca ttgatgtcat tggcagaggt gttcgtatac tttccactta
     3121 cgcgcagaat aaatctgatg aaatagaagt gaaatatcct tcagtacatg ttgcaccact
     3181 tcagaataac gatctactgg aagacttttt ctcacctgtc gcgcgcgatg gtgccagtat
     3241 gagggagatt cagataagag tccttaaagg actgtcgatg ctgagtaaag gttggcctga
     3301 gatatttgct gaagccgcac ataccctggc atttgaaaca ttagagcatg caactcgtgc
     3361 tgaccatata gattctgata gatatctaat aaaatcaact tattataatt tatttagtgg
     3421 cgaatattct aataaaaaaa catagctgca gagcaagcaa ttcggttgtt ggcaagtcaa
     3481 gggcaaagca ttcgcgtgag aatgggcgta tgctttgtgc caaaagcaaa cgttgctaat
     3541 tctgcactgt tcagcgatgg cgcagtagag acaggcactg ccactgtcca gtcggcaaaa
     3601 gcgaacactt tgccactgcg gggataaact gattgaagct ctcgcgcaaa atcacttatt
     3661 cttttgcgtt aataaaatgt aggagatggg tcattgatcc tacccacgta atatggacac
     3721 aggcctaagc gaggttct
//
LOCUS       DAZSIO010000255         3728 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZSIO010000255 DAZSIO010000000
VERSION     DAZSIO010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.259"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..224
                     /locus_tag="ACHX5T_003944"
     CDS             <1..224
                     /locus_tag="ACHX5T_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR8468906.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..479
                     /gene="hha"
                     /locus_tag="ACHX5T_003945"
     CDS             270..479
                     /gene="hha"
                     /locus_tag="ACHX5T_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR8468907.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            518..1108
                     /locus_tag="ACHX5T_003946"
     CDS             518..1108
                     /locus_tag="ACHX5T_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR8468908.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1348..1608
                     /locus_tag="ACHX5T_003947"
     CDS             1348..1608
                     /locus_tag="ACHX5T_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR8468909.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1832..1906
                     /gene="tap"
                     /locus_tag="ACHX5T_003948"
     CDS             1832..1906
                     /gene="tap"
                     /locus_tag="ACHX5T_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR8468910.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1899..2756
                     /gene="repA"
                     /locus_tag="ACHX5T_003949"
     CDS             1899..2756
                     /gene="repA"
                     /locus_tag="ACHX5T_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR8468911.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3457..3648
                     /locus_tag="ACHX5T_003950"
     CDS             3457..3648
                     /locus_tag="ACHX5T_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468912.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT         1020 a          907 c          934 g          867 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcggaat
      421 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
      481 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
      541 cattgttctg attattgcag cgctacttat ccggattatc ctcagaagtg taaatcagca
      601 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg actggtgatg cagagcgaat
      661 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
      721 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
      781 acgggtagcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
      841 gcagttattc aggatggtgt ctcagtggca tgttgatgtg gttatcgttg agctgcgttc
      901 gttcagtatt gttgcagcag tagagctgga tgatgccagc catttacgac cggaacgcag
      961 acgccgggat attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag
     1021 ccacgatgcc agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctga
     1081 tcagcagtca ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg
     1141 aaacgggaaa aagcaagttt tcctcgctcc cggcgtttcc ataactgaaa accatactat
     1201 ttcacagttt aaatcacatt aaacgacagt aatccccgtt gatttgtgcg ccaacacaga
     1261 tcttcgccac aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga
     1321 tccctgtttg agtattgagg aggcgagatg tcgcagacag aaaatgcagt gacttcctca
     1381 ttgagtcaaa agcggtttgt gcgcagaggt aagcctatga ctgactctga gaaacaaatg
     1441 gccgctgttg caagaaaacg tcttacacac aaagagataa aagtttttgt caaaaatcct
     1501 ctgaaagatc tcatggttga gtactgcgag agagagggga taacacaggc tcagttcgtt
     1561 gagaaaatca tcaaagatga actgcagaga ctggatatac taaagtaaag actttacttt
     1621 gtggcgtagc atgctagatt actgatcgtt taaggaattt tatggctggc cacgccataa
     1681 ggtggcaggg aactggttct gatgtggatg tacaggagcc agaaaagcaa aaaccccgat
     1741 aatcttcttt aactttggcg agtcagaaag attaccgggg cccacttaaa ccgtatagcc
     1801 aacaatcaag ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
     1861 gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt caacaaactt
     1921 attaccgcca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaaggt gcccggacgc
     1981 ttccgttctg cgggaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
     2041 ccattcatgt ggcacatgcc cgttcgcttg gtctgcgtcg gcgcatgcca ccggtactac
     2101 gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt tcactatgac ccactggcca
     2161 accgcgtcca gtgttccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     2221 ctgccggaaa actctccatc acccgggcca cccgggccct gacgttcctt gcagagctgg
     2281 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgata
     2341 tcacgttcac atctgcactg tttgctgccc tcgatgtatc agaggaggca gtggccgccg
     2401 cgcgccgcag tcgtgtggaa tgggaaaaca gacagcgcaa aaagcagggg ctggataccc
     2461 tgggtatgga tgaactgatg gcgaaagcct ggcgttttgt tcgtgagcgt ttccgcagtt
     2521 accagacaga gcttaagtcc cgtggaatga agcgtgcccg tgcgcgtcgt gatgcggaca
     2581 ggcagcgtca ggatattgtc accctggtga aacggcaact gacgcgtgaa atctcggaag
     2641 ggcgcttcac tgccagtcgt gaggcggtaa aacgtgaagt ggagcgtcgt gtgaaggagc
     2701 gcatgattct gtcacgtaac cgtaattaca gccggctggc cacagcttcc ccctgaaagt
     2761 gacctcctca gaataatccg gcacgcgccg gaggcattcg cacgcctgaa gcccgccagt
     2821 gcacaaaaaa tcagcgctac atacaaaaaa caacctcatc atccaccttc aggtgcatcc
     2881 ggtcccccct gtttttgata caaaacacgc ctcacagacg gggaattttg cttatccaca
     2941 ttaaactgca agggacttca ccataaggtt acaaccgttc acgtcataaa gcgccagacg
     3001 tcaaccctac agggtgcaat gtatctttta aacacctgtt tatatctcct ttaaactact
     3061 taattacatt catttaaaaa gaaaacctgt tcactgcctg tcctgtggac agacaaatat
     3121 gcacctccca ccgcaagcgg cgggccccca accgaagccg gtttagttac aacatgcaaa
     3181 cataaacccc taaaaaaccc aagtccaacg caaaactgaa accacaacgc ccccctcata
     3241 actgaaaagc gccgccgccc ccgcccaaag ggcgggaaca gagtcgcttt taattatgaa
     3301 tgttgtaact acatcatcat cgctgtcagt cttctggctg gacgtatcga atacacgctc
     3361 gtaagcggcc ctcacggccc gctaacgcgg agatacgccc cgacttcggg taaaaccctc
     3421 gtcgggacca ctccgaccgc gcacagaagc tctatcatgg ctgaaaacgg gtatggcttg
     3481 gcagggctgg ggatgggtaa ggtgaaatct gtcaatcagt accggctgac gccgggcttc
     3541 ggcggtttta ctccggtatc acatgtaacc acagcgtgcc gccttccatg ccgctggcgc
     3601 ggcatcagaa taatacaagc agcatttaat gcatttgcaa aagtgtgagt aatattaagt
     3661 ggtcgtttca gggatatcgt taagacttga gcgataggaa ttaaaacccc aaaaagatta
     3721 aaaaaaca
//
LOCUS       DAZSIO010000256         3663 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZSIO010000256 DAZSIO010000000
VERSION     DAZSIO010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.260"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(34..78)
                     /locus_tag="ACHX5T_003951"
     CDS             complement(34..78)
                     /locus_tag="ACHX5T_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468913.1"
                     /translation="MKRNDKILHYEGLN"
     gene            197..1921
                     /gene="ilvI"
                     /locus_tag="ACHX5T_003952"
     CDS             197..1921
                     /gene="ilvI"
                     /locus_tag="ACHX5T_003952"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR8468914.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            1924..2415
                     /gene="ilvN"
                     /locus_tag="ACHX5T_003953"
     CDS             1924..2415
                     /gene="ilvN"
                     /locus_tag="ACHX5T_003953"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR8468915.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            2427..2513
                     /locus_tag="ACHX5T_003954"
     CDS             2427..2513
                     /locus_tag="ACHX5T_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR8468916.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            2595..3599
                     /gene="cra"
                     /locus_tag="ACHX5T_003955"
     CDS             2595..3599
                     /gene="cra"
                     /locus_tag="ACHX5T_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR8468917.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT          849 a          879 c         1017 g          918 t
ORIGIN      
        1 tgctgtaagt tgtgggattc agccgattta ttatcaattt aatccttcgt aatggaggat
       61 tttatcgttt cttttcacct ttcctcctgt ttattcttat taccctgcgt ttatgtctct
      121 ggctgccaat tgcttaagca agatcggacg gttaatgtgt tttacacatt ttttccgtca
      181 aacagtgagg caggccatgg agatgttgtc tggagccgag atggtcgtcc gatcgcttat
      241 cgatcagggc gttaaacaag tattcggtta tcccggaggc gcagtccttg atatttacga
      301 tgcattgcat accgtgggtg gtattgatca tgtattagtt cgtcatgagc aggcggcggt
      361 gcatatggcc gatggcctgg cgcgtgcgac cggggaagtc ggcgtcgtgc tggtaacgtc
      421 gggtccaagg gcgaccaatg cgattaccgg catcgccacc gcttatatgg attccattcc
      481 attagttgtc ctttccgggc aggtagcgac ctcgttgata ggttacgatg cctttcagga
      541 gtgcgacatg gtggggattt cgcgaccggt ggttaaacac agttttctgg ttaagcaaac
      601 ggaagacatt ccgcaggtgc tgaaaaaggc tttctggctg gcggcaagtg gtcgcccagg
      661 accagtggtc gttgatttac cgaaagatat tcttaatccg gcgaacaaat taccctatgt
      721 ctgccctgag tcggtcagta tgcgttctta caatcccact actaccggac ataaagggca
      781 aattaagcgt gctctgcaaa cgctggtagc ggcaaaaaaa ccggttgtct acgtaggcgg
      841 tggggcaatc atggcgggct gccatcagca gttgaaagaa acggtggagg cgttgaatct
      901 gcccgttgtt tcctcattga tgggaatggg ggcgtttccg gcaacgcatc gtcaggcact
      961 gggcatgctg ggaatgcacg gtacctacga agccaatatg acgatgcata acgcggatgt
     1021 gattttcgcc gtcggggtac gatttgatga ccgaacgacg aacaatctgg caaagtactg
     1081 cccaaatgcc actgttctgc atatcgatat tgatcctact tccatttcta aaaccgtgac
     1141 tgcggatatc ccgattgtgg gggatgctcg ccaggtcctc gaacaaatgc ttgaactcct
     1201 gtcgcaagaa tccgcccatc aaccgctgga tgagatccgc gactggtggc agcagattga
     1261 acagtggcgc gctcttcagt gcctgaaata tgacactcac agtgaaaaga ttaaaccgca
     1321 ggcggtgatc gagactcttt ggcggttgac taatggggac gcttacgtga cgtccgatgt
     1381 cgggcagcac cagatgtttg ctgcgcttta ttatccattc gacaaaccgc gtcgctggat
     1441 caattccggc ggtctcggca cgatgggttt tggtttacct gcggcactgg gcgtcaaaat
     1501 ggcgttgcca gaagaaaccg tggtttgcgt cactggcgac ggcagtattc agatgaacat
     1561 ccaggaactg tctaccgcgt tgcaatacga gttgcccgta ctggtggtga atctcaataa
     1621 ccgctatctg gggatggtga aacagtggca ggacatgatc tattccggcc gtcattcaca
     1681 atcttatatg caatcactac ccgatttcgt ccgtctggcg gaagcctatg gtcacgtcgg
     1741 gatccagatc tctcatccgc atgagctgga aagcaaactt agcgaggcgc tggaacaggt
     1801 gcgcaataat cgcctggtgt ttgttgatgt taccgtcgat ggcagtgagc acgtctaccc
     1861 gatgcagatt cgcgggggcg gaatggatga aatgtggtta agcaaaacgg agagaacctg
     1921 attatgcgcc ggatattatc agtcttactc gaaaatgaat caggcgcgtt atcccgcgtg
     1981 attggccttt tttcccagcg aggctacaac attgaaagcc tgaccgttgc gccaaccgac
     2041 gatccgacat tatcgcgtat gaccatccag accgtgggcg atgaaaaagt acttgagcag
     2101 atcgaaaagc aattacacaa gctggtcgat gtcttgcgcg tgagtgagtt ggggcagggc
     2161 gcgcatgttg agcgggaaat catgctggtg aaaattcagg ccagcggtta cgggcgtgac
     2221 gaagtgaaac gtaatacgga aatattccgt gggcaaatta tcgatgtcac cccctcgctt
     2281 tataccgttc aattagcagg caccagcgat aagcttgatg catttttagc atcgattcgc
     2341 gatgtggcga aaattgtgga agttgctcgc tctggtgtgg tcggactttc gcgcggcgat
     2401 aaaataatgc gttgagaatg atctcagtgc gcaatttgca gcccaacatg tcacgttggg
     2461 ctttttttgc gaaatcagtg ggaacctgga ataaaagcag ttgccgcagt taattttctg
     2521 cgcttagatg ttaatgaatt taacccatac cagtacaatg gctatggttt ttacatttta
     2581 cgcaaggggc aattgtgaaa ctggatgaaa tcgctcggct ggcgggagtg tcgcggacca
     2641 ctgcaagcta tgttattaac ggcaaagcga agcaataccg tgtgagcgac aaaaccgttg
     2701 aaaaagtcat ggctgtggtg cgtgagcaca attaccaccc gaacgccgtg gcagctgggc
     2761 ttcgtgctgg acgcacacgt tctattggtc ttgtgatccc cgatctggag aacaccagct
     2821 atacccgcat cgctaactat cttgaacgcc aggcgcggca acggggttat caactgctga
     2881 ttgcctgctc agaagatcag ccagacaacg aaatgcgctg cattgagcac cttttacagc
     2941 gtcaggttga tgccattatt gtttcgacgt cgttgccacc tgagcatcct ttttatcaac
     3001 gctgggctaa cgacccgttc ccgattgtcg cgctggaccg cgccctcgat cgtgaacatt
     3061 tcaccagcgt ggttggtgcc gatcaggatg atgccgaaat gctggcggaa gagttacgta
     3121 agtttcccgc cgagacggtg ctttatcttg gcgcgctgcc ggagctttct gtcagcttcc
     3181 tgcgtgaaca aggtttccgt actgcttgga aagatgatcc gcgcgaagtg catttcctgt
     3241 atgccaacag ctatgagcgg gaggcggctg cccagttatt cgaaaaatgg ctggaaacgc
     3301 atccgatgcc gcaggcgctg ttcacaacgt cgtttgcgtt gttgcaagga gtgatggatg
     3361 tcacgctgcg tcgcgacggc aaactgcctt ctgacctggc aattgccacc tttggcgata
     3421 acgaactgct cgacttctta cagtgtccgg tgctggcagt ggctcaacgt caccgcgatg
     3481 tcgcagagcg tgtgctggag attgtcctgg caagcctgga cgaaccgcgt aagccaaaac
     3541 ctggtttaac gcgcattaaa cgtaatctct atcgccgcgg cgtgctcagc cgtagctaat
     3601 ccgcgaacaa aaatacgcgc caggtgaatt tccctctggc gcgtagagta cgggactgga
     3661 cat
//
LOCUS       DAZSIO010000257         3585 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZSIO010000257 DAZSIO010000000
VERSION     DAZSIO010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.261"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..439
                     /locus_tag="ACHX5T_003956"
     CDS             <1..439
                     /locus_tag="ACHX5T_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR8468918.1"
                     /translation="LTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQTLLL
                     REKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGVPQS
                     GEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            450..1901
                     /locus_tag="ACHX5T_003957"
     CDS             450..1901
                     /locus_tag="ACHX5T_003957"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFR8468919.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            1891..2961
                     /locus_tag="ACHX5T_003958"
     CDS             1891..2961
                     /locus_tag="ACHX5T_003958"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR8468920.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            2961..>3585
                     /locus_tag="ACHX5T_003959"
     CDS             2961..>3585
                     /locus_tag="ACHX5T_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFR8468921.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT          795 a          938 c          992 g          860 t
ORIGIN      
        1 tcttactgca agctgtattg acctgaatat tcagggcaat ggcgcttatt ccgttctgaa
       61 gcagttggcg acaatagcgt tacaaaacgg ttttatcacc gactcacacc agttcctgca
      121 aaccctgctg ctgcgcgaaa aaatgcactc tactggattt ggttccggtg tcgcagtgcc
      181 gcacggtaaa agcgcctgcg ttaaacaacc gttcgtatta ttcgcccgca aagcgcaggc
      241 tattgactgg aaagccagcg atggcgaaga cgtcaattgc tggatctgcc tcggcgtgcc
      301 gcaaagcggc gaagaggatc aggtcaaaat catcggcaca ctgtgtcgca aaattattca
      361 ccaggaattt attcatcaac tgcaacaggg cgataccgac caggtgctgg ccttgttaaa
      421 tcaaaccctc agctcataag gaagtggcga tggagtcatc cttacgtatt gtcgcgatca
      481 ccaactgccc cgccgggatc gctcacacct acatggtggc ggaagccctg gaacagaaag
      541 cccgttctct cggccatacc ataaaagtgg aaactcaagg gtccagtggc gttgaaaacc
      601 gcttatccag cgaagagatt gccgctgccg attacgtcat tcttgctacc gggcgtggcc
      661 tgagcggtga tgatcgcgcg cggtttgccg ggaagaaagt ttatgagatt gccatctccc
      721 tggcgttgaa aaatatcgac cagattttca gcgaattacc gacaaactcg cagctttttg
      781 ccgcagatag cggcgtgaag cttggcaaac aggaagtgca aagcggcagc gtgatgagcc
      841 acctgatggc gggcgtctct gcggcgctgc cgtttgtcat cggcggtggt atcctggtgg
      901 cgcttgccaa catgctggtg cagttcggtt tgccgtatac cgatatgtcg aaaggcgcgc
      961 cgtcgtttac ctgggtagtt gaatccatcg gttaccttgg cttcaccttt atgatcccca
     1021 tcatgggcgc ttatattgcc tcgtcgattg ccgataaacc ggctttcgct ccggcgtttc
     1081 tggtttgcta tctggcgaac gacaaagcgc tgctcggcac ccagtcgggt gcgggattcc
     1141 tcggcgcggt ggtgctgggg ctggcgattg gctatttcgt cttctggttc cgcaaagtgc
     1201 gccttggcaa agcattacaa ccgctgctcg gttcaatgct gatcccgttc gttactctgc
     1261 tggttttcgg cgtgctcact tactacgtta tcgggccggt gatgtccgac ctcatgggcg
     1321 ggctgctcca cttcctgaat accattcctc catcaatgaa gtttgcggca gcgtttctgg
     1381 tcggtgcgat gctggcgttc gatatgggcg gccccatcaa taaaaccgcc tggttcttct
     1441 gcttctcact gctggaaaaa cacatttatg actggtacgc catcgtcggc gttgtcgcgt
     1501 tgatgccgcc cgttgcggcg ggtcttgcga cgttcattgc gcccaaactg tttacccgac
     1561 aggaaaaaga ggctgccagt agtgccattg tggtcggtgc cacagtcgca accgaaccgg
     1621 ctatacctta cgctctggca gctccgttgc cgatgattac cgccaatacg ctggcgggcg
     1681 gcattaccgg cgtgctggtg atcgctttcg gaatcaaacg tctggcacct ggtttgggta
     1741 tctttgaccc gctgatcggc ctgatgtcgc cggtaggttc gttttatctg gtgctggcga
     1801 ttggcctcgc gctgaacatt tcattcatta tcgtcctgaa aggattgtgg ttacgccgca
     1861 aagcgaaagc cgcgcaacag gagcttgtcc atgaacattg agttactgca acagttgtgc
     1921 gaagccagcg ccgttagcgg cgatgaacag gaagttcgcg acatcctgat aaacacgctg
     1981 gaaccttgcg ttaatgaaat cacctttgat ggtctgggca gctttgttgc ccgtaaggga
     2041 aataaaggtc caaaagttgc cgttgtcggg catatggatg aagtcggctt tatggtcacc
     2101 cacatcgacg agagcggttt tctgcgtttc accaccattg gtggctggtg gaatcagtcg
     2161 atgctcaacc atcgggtaac catacgcaca cacaagggag tgaaaatccc tggtgtgatt
     2221 ggtttcgtcg cgcctcatgc gttaacggaa aagcaaaggc aacagccgct gtcatttgat
     2281 gagatgttca ttgatattgg cgcaaacagt cgcgatgagg tggaaaaacg cggtgtggcg
     2341 atgggcgatt ttattagccc ggaagccaat tttgcctgct ggggcgaaga taaagtggtc
     2401 ggcaaggcgc tggataaccg catcggctgc gcaatgatgg ctgaactatt gcagacggtg
     2461 aataatcccg aaattacgct gtatggcgtc ggcagcgtgg aagaagaagt tgggctacgc
     2521 ggggcacaaa cctcggcgga acacattaaa ccggatgtag tgatcgtgct ggataccgct
     2581 gtcgcaggcg atgttccggg cattgataac attaaatacc cgctgaaact gggccagggg
     2641 ccggggctga tgctgtttga caagcgctac ttccccaacc agaaactggt agcagcgtta
     2701 aaaaactgtg ccgcacataa cgatttaccg ctgcaatttt ccaccatgaa aaccggagcg
     2761 acggatggcg ggcgctacaa cgtgatgggc ggcgggcgtc cggttgttgc gctgtgcctg
     2821 ccaacccgct atctgcacgc taacagcggc atgatttcaa aagccgatta cgatgctctg
     2881 ctcacgttga tacggggttt tctgacgacc ttaaccgcgg agaaagtcaa cgcgtttagc
     2941 cagttccgtc aggtggatta atgctaaacg aacgccagtt aaagattgtc gatctgctgg
     3001 agcaacagcc gcgcacgcct ggtgagctgg cgcaacagac tggcgtttca ggcaggacca
     3061 tcctgcgtga tattgactat ctcaacttta cccttaacgg caaagcccgc attttcgcca
     3121 gtggcagtgc gggctatcag ctggaaatct tcgagcgccg cagctttttt cagttgctgc
     3181 aaaagcacga taacgacgat cggctgctgg cgctgttatt actgaatact ttcactcccc
     3241 gtgcgcaact cgcctcggcg cttaatttgc cagaaacgtg ggtagcagag cgtctgcccc
     3301 agttaaaaca gcgttatgaa cgcacttgtt gcctggccag ccgccctggt ttgggccatt
     3361 tcattgatga gacagaagag aaactcgtta tcttgctggc gaacttgctg cgcaaagatc
     3421 cgtttttaat tccgctggcg ggcataacac gagacaacct tcagcattta tccacggcct
     3481 gcgacaacca acaccgctgg ccgctcatac agggtgatta tctctccagc ctgattctgg
     3541 cgatttacgc cctgcgtaat caactgaccg atgagtggcc gcaat
//
LOCUS       DAZSIO010000258         3575 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZSIO010000258 DAZSIO010000000
VERSION     DAZSIO010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.262"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..620)
                     /locus_tag="ACHX5T_003960"
     CDS             complement(<1..620)
                     /locus_tag="ACHX5T_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8468922.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGIAKSQIEAAEISKKHLP"
     gene            866..1627
                     /locus_tag="ACHX5T_003961"
                     /pseudo
     CDS             866..1627
                     /locus_tag="ACHX5T_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(1734..2246)
                     /locus_tag="ACHX5T_003962"
     CDS             complement(1734..2246)
                     /locus_tag="ACHX5T_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468923.1"
                     /translation="MKKTLLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNT
                     IQPVKGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNN
                     PDHKLKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYT
                     IRTQAYIYAE"
     gene            complement(2385..3533)
                     /locus_tag="ACHX5T_003963"
     CDS             complement(2385..3533)
                     /locus_tag="ACHX5T_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR8468924.1"
                     /translation="MSITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQP
                     LGNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDR
                     KLSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAH
                     QSQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDG
                     TLALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTS
                     QLVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDG
                     IAYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT         1087 a          790 c          630 g         1068 t
ORIGIN      
        1 ggtaaatgct ttttcgaaat ttcagcggcc tctatttgag atttagcgat gccaatctcg
       61 ttaagattct ctgaaagata taaatcacag gtgtcaatct tacctgattt aaccaatgga
      121 taatccgtta tactgtaggt atctttaaaa gccacggcaa acatttcaat aataacaatt
      181 actaccacaa gcgaaatcaa aatgcgaaaa agaggaatac tccttttgcg ctctactttt
      241 tcaacaacat cttcttgtaa atgagaatca tcataagatt gtttacttat tgttatgttt
      301 tcactgatgt aaaagcctac tttgggtatc gtaagaatca cattttcttc aacaccaata
      361 tttttaaaac ttttgcgtaa cagggatata tattggttca atgtgttgtt tgacagatca
      421 aatgaaaatc tctccaaaac gctatcaatg attgagttcc tgtcaaccgc tccaccatta
      481 ctttccagta acactgccaa aatacacccc gccgtagctg gaaaatttat ctcatcctgg
      541 gtatttacat tcgagagcga gcatttgttc ggatcaaaaa taatcgcgtc attgataatg
      601 aaaaaacatc tccgctccat gcaggcaaca acacaaaata agattttgtc attttagcct
      661 attcctggtg gaactgacac aagaaactaa aatacaaata taaccctgaa aaagtcatat
      721 aaaacttata tccacagaat aaaaaatcaa acatgtcagt tttatcaata taaagtatcg
      781 caaggcgaaa agtgatgcaa gaatactcct aagtaaatga aagcgcacca catgaaatta
      841 tcaacacatg taatttcatc attaggtgct atcgaaaata actcaggagc tacttaagat
      901 gaataatgtc ttactcatga gacccgatga ctcagaattt atgatacctg atgactggtc
      961 agggctaaac gcaaaaggca ttacatacga acaagataat agagtgacag agtcttttct
     1021 gccttttaaa tctttttatt gttcaccggt gtgcacgaca gttgaactca tctcataaaa
     1081 taattgtttt gctatgggta tccgctacct tctggaaaag aaaagagact taaggctaaa
     1141 tcactttaaa gccttctctc ataaatgcga gacaaaacct gacgtgttaa ttatgaccgg
     1201 gtgcaccgtc gctgatactt atagtatatt ctcacgcatt gttgaagaaa atataatgaa
     1261 aacgatgaca cctatcgtca tcattactgg gcatcactca gaagatcttt gttctctggc
     1321 atcgaaactg ggaggagtta aaataatatc accgacagaa aagattgcaa aaatagaaaa
     1381 cgctatcaat gatgctttta acagcaagcc taaaaaaata agatttttaa cagatatact
     1441 ctctcctcaa caaattgaga ccatgaaaat ggttgcgcag ggtagatccg ttaatgagat
     1501 ttcagccgca atgaatatta gcatcaagac cgtgtatgca cataaagttc gtgctatcaa
     1561 taagctggga attaagaaaa aagtttatga agcgcttttt tataaagcgc taattcagtt
     1621 caactgatgt aaaaatcacc ctcgcttaat aaataatata ttctatgtct ttttttataa
     1681 gttaaaaaag gcgggatatc aaatcccgcc atgaaattaa ctaatcatct gcattattct
     1741 gcgtagatat aagcctgggt acggatggtg taagagtctg gctcaacaac ctgttctttc
     1801 atatgggaaa cgacgcgata ggcaccacct gttccagtat ttttcagcat catataacgc
     1861 ttaccgccaa tctcttcgga ggcagctgaa ggaggatcct gctcaagcgt tgtcagggaa
     1921 actttcagtt tatggtccgg gttattgtca ccagaaatta ctgcatccgc tgaagtactg
     1981 ctctccgcat aaatatcatc cgggaaagtt acagcatagt cgtggctctg cccatcagat
     2041 gcgccaacaa tacctgttgc caaaggcgtc catgcagtta acgtaccgct atatacccct
     2101 ttcactggtt gaatggtatt attaacaacg ataggcttgg tcaaagtaag attagctgac
     2161 gcttcagtac cctggcttac taaagttccc gcctgagaag caaaagctgt ccccgccagc
     2221 atgattgcta acaatgtttt cttcattata ttttctccat gattttcatt tacactgcaa
     2281 aatgcagttt ctttctttgc atttacgtgc tatcgcgaaa atgcttccac caaataatgg
     2341 aaacatcttc acaaaaataa attatgagtt tcttaattta gaaactataa acatgtcgcc
     2401 gctatctggt ataaccctga tgcacttttc ttcgcaggaa gttggtattt tgcgatacag
     2461 gattgatctt tagaataacc ccatttcaca ttaaactcgc cttccgcact caagccagaa
     2521 acataagcaa taccatcttc acctacaatg ccggccatat tttcagtgtc tttgctataa
     2581 ctaatcacag cgccgaaggg gatgggttta ccctctttct gttttaaggt tactaatcct
     2641 ttatccccgc tcttcactgc caatgtcgca ggcacaacgg ctccagctgt cggtacaacc
     2701 aattgactag tttgtggtaa gtcgtaattc ttacctatcg ttttttcatc cagaataaca
     2761 tcgtctcttc ggtatggctg cactggcagt attgcatacc cacgaaaatc agtactcagt
     2821 cccggccagc cattaacgtt aacccccgtt gctccgggtg cctctataag ggctaatgtc
     2881 ccatccacat tttgaccaaa tgtaagtcca tcccttgtaa taagtatccc accgcttaaa
     2941 tttgcaccta gctgtttcat gttttcgctg gagctgtaat ggctactaaa ctgaccatat
     3001 gatccatttt tgccaacttg cacaatgttc atcgtgctct tcggttgtga ctgatgagcg
     3061 ttagttatat ttgtcgctac atcccaccat acaccaccac tattctgacc tgataatgaa
     3121 acctgatagt tattaccctg ctctcccgaa cgactcatct gtgaagacgc ataaatttga
     3181 ttttcgtcac ccagcgataa tggcactgaa atacccacgc ttaatttccg atctactttc
     3241 catttatgcg atgtcagcaa gtcgtttttt gcccagttaa tggagaatgt tatgcctctc
     3301 cataccgtac tataactcag gctggtagac cgcgtgtttc ctgtgccccg ggaagaatta
     3361 tacctcgtgg tattgaagtt caaattcccg atattaccaa ggggctggga taaggatatc
     3421 gtagagccat cgtgactatt cttatctctt tttctgttcc gttcatcctg tgcacttact
     3481 gcttcagtca gtgtggcata acctgatgca taacggttcg tggttataga caatagactg
     3541 gccccctgaa tctccagaca accagtatca cttat
//
LOCUS       DAZSIO010000259         3549 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZSIO010000259 DAZSIO010000000
VERSION     DAZSIO010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.263"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="ACHX5T_003964"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="ACHX5T_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR8468925.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="ACHX5T_003965"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="ACHX5T_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR8468926.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="ACHX5T_003966"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="ACHX5T_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR8468927.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIKNYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          772 c          820 g          951 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tttataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggcg
//
LOCUS       DAZSIO010000260         3541 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZSIO010000260 DAZSIO010000000
VERSION     DAZSIO010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.264"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            7..864
                     /gene="yibB"
                     /locus_tag="ACHX5T_003967"
     CDS             7..864
                     /gene="yibB"
                     /locus_tag="ACHX5T_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFR8468928.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            1139..2335
                     /gene="kbl"
                     /locus_tag="ACHX5T_003968"
     CDS             1139..2335
                     /gene="kbl"
                     /locus_tag="ACHX5T_003968"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR8468929.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            2345..3370
                     /gene="tdh"
                     /locus_tag="ACHX5T_003969"
     CDS             2345..3370
                     /gene="tdh"
                     /locus_tag="ACHX5T_003969"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR8468930.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
BASE COUNT          897 a          764 c          914 g          966 t
ORIGIN      
        1 ttagtaatga aatcatccac cacaattatc actgcatatt ttgacattgg tagaggggat
       61 tggactgcta ataaagggtt ccgtgaaaaa ctagctcgtt cagttgatgt ttattttagt
      121 tactttgagc gtctggccgc acttgaaaat gagatgatta ttttcacttc tcccgatttg
      181 aaaccaagga ttgaagcgat tcgcaacgga aaaccaacaa cggttatcgt tatcgatata
      241 aaaaagaaat ttagatatat cagaagtcgt atcgaaaaaa ttcaaaaaga tgagtcattt
      301 acaaatagac ttgagcctcg acaattaaaa aacccagagt actggtcacc agagtatgtt
      361 ttggtatgta accttaaggc ctattttgta aataaagcta tcaacatggg tttagtgaaa
      421 acgcctcttg ttgcgtggat agattttggc tattgccgta agccaaatgt aactcgagga
      481 ttgaaaattt gggatttccc atttgatgaa aataagatgc atcttttcac tataaaaaaa
      541 gggcttactg ttacatctca acaacaggct tttgatttca tgattggcaa tcatgtatat
      601 attattgggg gcgcaattgt tggttcacag cataaatgga aagaattcta taaattagtt
      661 ctggaaagtc aaaaaataac actaaacaat aacattgtgg acgatgacca gggcattttt
      721 gtcatgtgct attacaaacg ttccgatctc ttcaacctaa actatctggg gagaggaaaa
      781 tggttcgatt tgtttcgctg cttcaggagt aacactttag gggcaaaaat gcaggcgctg
      841 agaattttcc tttcaagaaa ataggtttcc ctccattagt gggtagcgtt ttgatgccaa
      901 ctgagatagg aaaaatgatg ctttggcatc attttttctt tttatatcaa tgcagtaaaa
      961 tcactcatca ttatgctcag ccaaaactgt acagtttttc tactatctgt aggtgttgag
     1021 agacatcaag atacacgtta tttatcctga attttgcaga agtgttaacg catcatctcg
     1081 tcgcgaccta taagtttggg taatatgtgc tggaatttgc cctgtctgga gaatcgcaat
     1141 gcgtggagaa ttttatcagc agttaaccaa cgatctggaa accgcacggg cggaagggtt
     1201 gtttaaagaa gagcgcatta ttacgtctgc gcagcaagca gatatcaccg tggctgatgg
     1261 aagccacgtc attaactttt gtgccaacaa ctatctcggg ctggcgaatc atccggatct
     1321 gattgcggca gcaaaggcgg gaatggattc tcacggtttc ggcatggctt cggtgcgttt
     1381 tatttgcggc actcaggaca gccataaaga gcttgaacaa aaactggcgg cattcctggg
     1441 gatggaagat gcgattctct actcttcctg ctttgatgct aacggtggcc tgtttgaaac
     1501 gctgctgggc gcggaagacg ccattatctc cgacgcactg aaccacgcgt ctattattga
     1561 tggtgtgcgt ctgtgtaaag ctaaacgcta tcgctatgcc aacaacgata tgcaggagct
     1621 tgaagctcgt ctgaaagaag cgcgtgaagc cggtgcgcgt catgtgctga ttgccaccga
     1681 tggtgtgttc tcgatggacg gcgtgattgc caatctgaag ggcgtttgcg atctggcaga
     1741 taaatacgat gccctggtga tggtagacga ctcccacgcg gtcggttttg tcggtgaaaa
     1801 tggtcgtggt tcccatgaat actgcgatgt gatggggcgt gtcgacatca tcaccggcac
     1861 gctcggtaaa gcgctgggcg gggcttctgg tggttatacc gcggcgcgca aagaagtggt
     1921 tgagtggctg cgccagcgtt ctcgtccgta cctgttctcc aactcgctgg caccagcaat
     1981 tgtcgccgct tccatcaaag tgctggagat ggttgaagcg ggtagcgagt tgcgtgaccg
     2041 tctgtgggcg aacgcgcgtc agttccgtga gcaaatgtcg gcggcgggct ttaccctggc
     2101 gggagccgat cacgccatta ttccggtcat gcttggtgat gcggtagtgg cgcagaaatt
     2161 tgcccgagag ctgcaaaaag agggcattta cgtcaccggt ttcttctatc cggtcgttcc
     2221 gaaaggtcag gcgcgtattc gtacccagat gtctgcggcg catacccctg agcaaattac
     2281 gcgtgcagta gaagcattca cgcgtattgg taaacaactg ggcgttatcg cctgaggatg
     2341 tgagatgaaa gcgttatcca aactgaaagc ggaagagggc atctggatga ccgacgtccc
     2401 tgtaccggaa ctcgggcata acgatctgct gattaaaatc cgtaaaacag ccatctgcgg
     2461 gactgacgtt cacatctata actgggatga gtggtcgcaa aaaaccatcc cggtgccgat
     2521 ggtcgtgggc catgaatatg tcggtgaagt ggtaggtatt ggtcaggaag tgaaaggctt
     2581 caaaatcggc gatcgcgttt ctggcgaagg ccatatcacc tgcggtcact gtcgcaactg
     2641 tcgtggcggt cgtacccatc tgtgccgcaa caccattggt gtcggtgtta accgtccggg
     2701 ctgcttcgcc gaatatctgg tgattccggc gttcaacgct ttcaaaatcc ccgacaatat
     2761 ctccgacgac ctggcttcca tttttgatcc cttcggtaac gccgtgcata ccgcgctgtc
     2821 gttcgatctg gtgggcgaag atgtgctggt ttctggtgca ggtccgatag gtattatggc
     2881 tgcggcggtg gcgaaacacg ttggtgcacg caatgtggtg atcaccgatg ttaacgaata
     2941 ccgccttgag ctggcgcgca aaatgggtat cacccgtgcg gttaacgtcg cgaaagaaaa
     3001 ccttaatgat gtgatggctg aactgggcat gaccgaaggc tttgatgtcg gtctggaaat
     3061 gtccggcgcg ccgccagcgt ttcgtaccat gctcgatacc atgaaccacg gcggtcgtat
     3121 tgcgatgctg ggtattccac cgtctgatat gtctatcgac tggaccaaag tgatctttaa
     3181 aggcttgttc attaaaggca tttacggtcg tgagatgttt gaaacctggt acaagatggc
     3241 ggcgctgatt cagtctggcc tcgatctctc gccgatcatt acccatcgtt tctctatcga
     3301 tgatttccag aagggctttg acgctatgcg ttcgggccag tccgggaaag ttattctgag
     3361 ctgggattaa cacgaacaag ggctggtatt ccagcccttt tatctgagga taatctgtta
     3421 attatgtaaa atcctgtcag tgtaataaag agttcgtaat tgcgctgatc ttttatatag
     3481 ctgttctcat tatctctcta ccctgaagtg actctctcac ctgtaaaaat aatatctcac
     3541 a
//
LOCUS       DAZSIO010000261         3529 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZSIO010000261 DAZSIO010000000
VERSION     DAZSIO010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.265"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..721)
                     /locus_tag="ACHX5T_003970"
     CDS             complement(<1..721)
                     /locus_tag="ACHX5T_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR8468931.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKALN"
     gene            complement(844..1296)
                     /gene="tabA"
                     /locus_tag="ACHX5T_003971"
     CDS             complement(844..1296)
                     /gene="tabA"
                     /locus_tag="ACHX5T_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR8468932.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(1441..2034)
                     /locus_tag="ACHX5T_003972"
     CDS             complement(1441..2034)
                     /locus_tag="ACHX5T_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR8468933.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            2105..2818
                     /gene="bdcA"
                     /locus_tag="ACHX5T_003973"
     CDS             2105..2818
                     /gene="bdcA"
                     /locus_tag="ACHX5T_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR8468934.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            2950..3345
                     /locus_tag="ACHX5T_003974"
     CDS             2950..3345
                     /locus_tag="ACHX5T_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR8468935.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
BASE COUNT          942 a          839 c          758 g          990 t
ORIGIN      
        1 tattcaatgc tttttctaat aaatccgtaa tcgtagaatc aactgaacct ttacaactca
       61 cctgcgagtt atttaactcc tgcgaattat ttaacttctg tgaattattt aactcctgcg
      121 aatcatttaa ctcctgtata ttatttaact tctgatgatt atttaataga cgcgctattt
      181 tcttgttagc ttcgcgttgg taacacgcca gaaccttgtt agtaatcagg tgtaatgaac
      241 tatcatcttt tctttcaaaa gacttccctt cataacgatt agtcgaacca tcagattgtt
      301 taaatataat gactgtatgt ccttcacgaa tccctttagt agaagctgag gcaagtgtaa
      361 tttctatctt caccttagat aaatccgctc gctccccttc aggattattc atgtaaaact
      421 tcattttttc attgccatca gtatctttag aataagatga gaactcaata tgagccatca
      481 tatttctacc cgcttcgttg gagcgtttgc taaaatccac ataacccagg gtaaatatct
      541 ttgccaaaaa ctccaaaaat gctctaggag aaagaatatt gcttctgcta ttctgccccc
      601 cggcaattcg ggcaaaatca ttcattccca cataccaggt atctaaggct tcattttttt
      661 gtttaaaatt gtcggctaat gtaatgtgtt gagaataatt caaatctgat attttgccca
      721 tgacgaactc cttgagttaa taagaacgtc taagcattac aaattttact cacaaaaaaa
      781 tatcaaaaaa cgctcttttc acaaactcgc cgtaaacctg gagttattta cggcgaaaag
      841 tcatcacgcc atcagcatct tcactactgc tttgcgaacc tgggctggcg cgcccactgc
      901 gcacagcggt ttatgcactt cccccggata aaacacaaca aaatcacctt catttaggat
      961 aactgttttc tcatcaacgc cttccggcaa aaatgcgatg tctttatcag ctaaccagtc
     1021 ggtatccggc gcgcctgcag gttgcgtgct gaaggtcatg ccttcctgac cttttaacac
     1081 aatctgaatg tcaagatagc gggcatggta ctccgcacgg cgagcttcgt acggctcggt
     1141 catatcttcc gagataagat aaaacagacg attgccttcg atatcatgct tgccctttgg
     1201 cgtttctgcc gtaacgtgtg ctttgatatg ctcaatcgcc tggcgtaact cctggggtag
     1261 ccacggctga agattatgaa tatttccgat gatcatcttt tccactcctt aattaaaaca
     1321 atgtttcatt tctatcgtta tacgaaaaga tgatcaattc cgccagcctt aacgcgtgaa
     1381 ttattgtctt atcacaactt atcggcaggc gcgttcccgc ctttcggatc aaggccagca
     1441 tcactccttg agaagagttt ttatcgcttc ccctgccagg gcagctgttg cacagagttg
     1501 ttcgatggag tgcccctccc gtgccttcgc agaaagccct gacatcacgg tactcataaa
     1561 atcagtcaca cattgtgcgc tttgtggatg ccgcctggcg atatagtcat aaatggtcgt
     1621 ttctgcggcg tgataatatt gaatggcaat atcacgcgct tgtggatcat gactatgaat
     1681 accttcaaga accatacagc cagcgcagcc gccgttttgg ctatatctgc gcgccgcttc
     1741 ttttaatacc tcaaccaggc actcgcctac cggacgatca tcacgaagaa tatcggcaag
     1801 cggaatagct tccgtaccga catattcatt gagtacacgg ctaaataacc cagccttact
     1861 gccaaaagcc gcgtagagac tcggggggtt aataccaaga taatcagtaa cttcagagac
     1921 acttactgca tcgaaacctt tttggtgaaa aagcactttt gccgcagaga ctgcctgctc
     1981 aggagcgaaa cgtctgggac gacctggaac gcgagattgt tttttagtga ccattacaaa
     2041 acctgttgac agaaagttaa aacagttttg taatgcatgt tacataataa atcaaggagt
     2101 ccttatgggc gcttttaccg gtaagacagt tctcatcctc ggtggcagtc gtggaatcgg
     2161 tgccgctatc gtacgtcgtt tcgtcaccga tggggccaat gtacgattca cctatgcggg
     2221 gtcgaaagat gccgctgaac gtctggcaca agagactgga gcgacagcag tattcacaga
     2281 tagtgctgac agagacgctg ttattgatgt cgttcgtaag agcggcgcat tggatatcct
     2341 ggtggtaaat gcaggtattg gcgtctttgg cgatgccctg gaattaaatg ccgacgatat
     2401 tgatcgcctt ttcaaaatca atattcatgc tccttatcat gcctctgttg aagccgcccg
     2461 gcagatgccc gaaggcgggc gcatcttaat catcggctcc gtgaatggcg atcgtatgcc
     2521 tgttgcaggc atggctgctt atgccgccag taaatctgcc ctgcaaggca tggcgcgcgg
     2581 gctggcccgt gattttggac cgcgagggat caccatcaac gtcgtccagc caggaccaat
     2641 tgataccgac gttaatcccg ccaacgggcc aatgcgcgat atgttgcata gttttatggc
     2701 catcaaaaga catgggcaac cggaagaggt cgctggtatg gtcgcatggt tagcagggcc
     2761 agaagccagt tttgttaccg gcgcgatgca taccattgat ggcgcgtttg gcgcataacc
     2821 gactacgctc aattaagccc agccattatc catgatgtct gggttttgtt tactaacgtc
     2881 gtccccctaa aagcggctcc cgataaatat aaatcttctg ttcatgtcgt aaatcttgag
     2941 gataaccaaa tggtagaaag aaccgctgtt ttccctgctg gccgacattc actgtatgct
     3001 gagcatcgtt attctgcggc tattcgttcc ggcgatttgc tgtttgtttc cgggcaagta
     3061 ggaagtcgag aggacggaac accagaaccc gattttcagc aacaagtcag actggccttt
     3121 gataatttgc atgcgaccct ggcagctgcg ggatgcactt ttgacgatat cattgatgtt
     3181 acgagctttc ataccgatcc ggaaaaacaa tctgaagaca tcatgacggt gaaaaatgaa
     3241 atatttagcg ccccacctta tccaaactgg acggcggtgg gtgttacctg gctggcaggc
     3301 tttgattttg aaattaaagt gatagcgcgt atccctgagc tataagcaat agtgttagcc
     3361 gttcgttttc accctccgcc ctataagtcg gatgaatgga ataaaatgca tttctgattg
     3421 cgtgaaaatg aaaaagaaaa aagcaggaaa tgtctgcaat tattgatacc gaacgacact
     3481 tcccctccag aatcatagaa aataaaaatg catatatttt gacttttaa
//
LOCUS       DAZSIO010000262         3524 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZSIO010000262 DAZSIO010000000
VERSION     DAZSIO010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.266"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..77
                     /locus_tag="ACHX5T_003975"
     CDS             <1..77
                     /locus_tag="ACHX5T_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR8468936.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            435..1493
                     /gene="rfaQ"
                     /locus_tag="ACHX5T_003976"
     CDS             435..1493
                     /gene="rfaQ"
                     /locus_tag="ACHX5T_003976"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR8468937.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1490..2614
                     /locus_tag="ACHX5T_003977"
     CDS             1490..2614
                     /locus_tag="ACHX5T_003977"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR8468938.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2607..3404
                     /gene="rfaP"
                     /locus_tag="ACHX5T_003978"
     CDS             2607..3404
                     /gene="rfaP"
                     /locus_tag="ACHX5T_003978"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR8468939.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT          937 a          764 c          851 g          972 t
ORIGIN      
        1 cactgtcgtt ctcaaaatcg gtggagctgc atgacaaggt catcgggcat tatctgaaca
       61 taaaacacta tcagtaagtt ggagtcatta cccgtttttg acttaaatag caatttaccc
      121 gccaattctt gttgaacatc tatacttttt ttgtattttt atgcgattca ttgatttctg
      181 ctgtacgtgc aggaaggaat cgcagagcaa cagtgaaaga atatggttgc taatgatttt
      241 atttgattga tttctttatt gaaaatcaac aatgtgctta aaaatcattc gggtcggatt
      301 gccgcgaaag aagtgataca ctagcgcgtc aatatcagtg cggtatcagt attcaggtag
      361 ctgttgagcc tggggcggta gcgtgctttt tctgcttcac cttaatcgga taatctcaac
      421 aaaaagagtt acttgtggat aagccatttc gaagaatttt gctcattaag atgcgttttc
      481 atggggatat gttattaact actcccgtca ttagttctct gaaaaaaaat taccctgacg
      541 caaaaatcga tgtgctgctt tatcaggaca ccatcccgat cctgtctgaa aatccagaga
      601 ttaacgcgct ctacggcata aaaaataaaa aagcaaaagc ctcagaaaaa attgccaact
      661 tttttcatct catcaaggta ttacgtgcca ataagtatga ccttatcgtc aatcttaccg
      721 atcaatggat ggttgctata ctggttcgct tattaaatgc tcgtgtgaaa atttcccagg
      781 attatcatca tcggcagtct gctttttggc gtaaaagttt cacccatttg gtgccgttgc
      841 agggtggaaa tgtggtggaa agtaacttat ccgtgctgac accattggga cttgattcgt
      901 tggtgaagca gacaaccatg agttacccgc ctgcaagctg gaaacgtatg cgtcgcgaac
      961 ttgatcacgc tggtgttgga caaaattatg tggttatcca acctacggcg cggcaaatct
     1021 tcaaatgctg ggacaacgcc aagttttccg ctgtgattga tgccttacat gctcgtggtt
     1081 atgaagtcgt tctgacgtcc ggcccagata aagacgatct ggcctgcgtc aatgaaattg
     1141 cgcagggatg ccagacgcca ccagtaacgg cgctggctgg aaaggtgacc ttcccggaac
     1201 ttggtgcgtt aatcgatcat gcgcagctgt ttattggcgt tgattccgca ccggcgcata
     1261 ttgccgctgc agttaatacg ccgctgatat cgctgtttgg cgcaacagat catattttct
     1321 ggcgtccctg gtcaaataac atgattcaat tctgggcggg agattaccgg gaaatgccaa
     1381 cgcgcgatca gcgtgaccga aatgagatgt atctttcggt tattccggcg gcagatgtca
     1441 ttgctgctgt cgataaatta ctgccctcct ccacgacagg tacgtcgtta tgatcgttgc
     1501 tttttgttta tataaatatt ttccctttgg cggtttgcag cgcgatttta tgcgtattgc
     1561 tcagacagtc gccgcccgag gtcatcatgt tcgggtttat acccagtcgt gggaaggcga
     1621 atgccctgat gtatttgaag tgatcaaagt gccggttaaa tcgcatacca atcacgggcg
     1681 caatgcggag tattttgcct gggtgcaaaa acatttacgc gaacatcccg tcgataaagt
     1741 cgttggattc aacaaaatgc cggggctgga cgtttattat gccgctgatg tttgttatgc
     1801 cgagaaagta gcgcaggaaa aaggcttttt ctatcgcctg acgtcacgtt atcgccatta
     1861 tgccgccttt gagcgggcaa ccttcgaaca gggcaagccg acacagctgc tgatgctgac
     1921 agataagcaa atcgccgatt tccagaaaca ttatcagact gaagcggagc gttttcatat
     1981 tctgccaccg gggatttatc ctgatcgtaa atatagccag cagccagcca atagccgtga
     2041 aatcttccgt aagaagaatg gaataaccga acaacaatat ttattgttgc aggtcggttc
     2101 agacttcacg cgtaaaggtg ccgatcgttc cattgaagca cttgcttcgt taccggattc
     2161 gctgcgccac aacacattgc tatatgttgt tgggcaggat aaaccgcgaa aatttgaggc
     2221 actggcagaa aaacgcggcg tgcgcagtaa tgttcacttc ttctcggggc gcaacgatgt
     2281 ctcggaatta atggcggcgg cggatttatt actgcatcct gcctaccagg aagcggcggg
     2341 aattgtgctg ctggaagcga ttactgcagg attaccggta ctaacaactg ccgtttgtgg
     2401 ctatgcgcat tatattgtcg acgctaattg cggcgaggct attgctgagc cattccgcca
     2461 ggaaacattg aatgagattt tacgcaaagc gttaacgcaa tcttcattgc gccaggcttg
     2521 ggcggaaaat gcgcgacatt atgctgatac acaagattta tacagtctgc cagagaaagc
     2581 ggcggatatc ataacgggtg gtctggatgg ttgaacttaa agagccgttt gccacgttat
     2641 ggcgcggcaa agatcctttt gaggaagtta aaaccttgca gggtgaggta tttcgtgaac
     2701 tggaaactcg ccgtactctg cgctttgaaa tggcgggaaa aagctatttt ctcaaatggc
     2761 atcgcggcac gaccctgaaa gagataatca aaaatttact ctcattgcgg atgccagtat
     2821 taggcgcaga ccgcgaatgg aatgcgattc atcgactgcg ggatgtcggc gttgatacta
     2881 tgtatggggt ggcattcggc gaaaaaggca tgaatccgct gaccagaacc tcgtttatta
     2941 ttaccgaaga tctgacacca accatcagtc tggaagatta ctgtgctgac tgggcgacca
     3001 acccaccaga tgttcgcgta aagcgtatgc ttattaagcg tgtcgcgacg atggtgcgcg
     3061 atatgcatgc tgcgggcatt aaccatcgtg actgttatat ctgtcatttc ctgctgcact
     3121 tgcctttttc cggtaaggaa gaggagttaa aaatttcggt aattgaccta caccgggcgc
     3181 agcttcgcac gcgcgttcca ggtcgttggc gcgataaaga tcttataggg ctttattttt
     3241 cttcaatgaa tattggcctg actcagcggg atatctggcg ctttatgaag gtgtattttg
     3301 ccgccccgct taaagacatt ctcaagcagg aacaaggact gctgtcgcaa gcagaagcaa
     3361 aagccacaaa aatcagggaa agaacgattc gaaaatcgtt gtaacccgta acttattttt
     3421 gccaaaattt tggatacaga ataaatatgt ctcaactcaa tgatagtgac atcatccttt
     3481 ttgagtataa ttttcattat caaaatataa gatctaaaaa tact
//
LOCUS       DAZSIO010000263         3517 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZSIO010000263 DAZSIO010000000
VERSION     DAZSIO010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.267"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..1138
                     /gene="yiaY"
                     /locus_tag="ACHX5T_003979"
     CDS             <1..1138
                     /gene="yiaY"
                     /locus_tag="ACHX5T_003979"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFR8468940.1"
                     /translation="FFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTKLGMAG
                     DVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHDCAKGI
                     ALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNKMAIVD
                     KHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALKAVTMI
                     AENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLPHGVCN
                     AVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKVDIPAG
                     LRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            1303..2841
                     /gene="aldB"
                     /locus_tag="ACHX5T_003980"
     CDS             1303..2841
                     /gene="aldB"
                     /locus_tag="ACHX5T_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFR8468941.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3060..3203)
                     /locus_tag="ACHX5T_003981"
     CDS             complement(3060..3203)
                     /locus_tag="ACHX5T_003981"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468942.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            3386..>3517
                     /locus_tag="ACHX5T_003982"
     CDS             3386..>3517
                     /locus_tag="ACHX5T_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR8468943.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
BASE COUNT          876 a          841 c          929 g          871 t
ORIGIN      
        1 tttctttatt ccttctgtga atgtcatcgg cgctgattca ttgactgatg caatgaatat
       61 gatggcagat tatggattta cccgtacctt aattgtcact gacaatatgt taacgaaatt
      121 aggtatggcg ggtgatgtgc aaaaagcact ggaagaacgc aatattttta gcgttattta
      181 tgatggcacc caacctaacc ccaccacgga aaacgtcgcc gcaggtttga aattacttaa
      241 agaaaataat tgcgatagcg tgatctcctt aggcggtggt tctccacacg actgcgcaaa
      301 aggtattgcg ctggtggcag ccaatggcgg cgatattcgc gattacgaag gcgttgaccg
      361 ctctgcaaaa ccgcagctgc cgatgatcgc catcaatacc acggcgggta cggcctctga
      421 aatgacccgt ttctgcatca tcactgacga agcgcgtcat aacaaaatgg cgattgttga
      481 taagcacgtg actccgctgc tttctgtcaa tgactcctct ctgatgattg gtatgccgaa
      541 gtcactgacc gccgcaacgg gtatggatgc cttaacgcac gctatcgaag cgtatgtttc
      601 tattgccgcc acgccgatca ctgacgcttg tgcactgaaa gccgtgacca tgattgccga
      661 aaacctgccg ttagccgttg aagatggcag taatgcgaaa gcgcgtgaag caatggctta
      721 tgcccagttc cttgccggta tggcgttcaa taatgcttct ctgggttatg ttcatgcgat
      781 ggcgcaccag ctgggcggtt tctacaacct gccacacggt gtatgtaacg ccgttctgct
      841 gccgcatgtt caggtattca acagcaaagt cgccgctgca cgtctgcgtg actgtgccgc
      901 tgcaatgggc gtgaacgtga caggtaaaaa cgacgcggaa ggtgctgaag cctgcattaa
      961 cgccatccgt gaactggcga agaaagtgga tatcccggca ggcctacgcg acctgaacgt
     1021 gaaagaagaa gatttcgcgg ttctggcgac taatgccctg aaagatgcct gtggctttac
     1081 taacccgatc caggcaactc acgaagaaat tgtggtgatt tatcgcgccg caatgtaatc
     1141 atcattccaa caacggctgg caaattgtca gccgcttttt caactatctc tgtaatcctt
     1201 gcccgtaaat tcgtgatagc tgtcgtaaag ctgttaccga ctggcgaaga tttcgccagt
     1261 cacgtctacc cttgttatac ctcacaccgc aaggagacga tcatgaccaa taatccccct
     1321 tcagcacaga ttaagcccgg cgagtatggt ttccccatca agttaaaagc ccgctacgac
     1381 aactttattg gcggtgaatg ggtagcccct gccgatggtg agtattacca gaatctgacg
     1441 ccggtgaccg ggcagctgct gtgcgaagtg gcgtcttcgg gcaaacgaga catcgatctg
     1501 gcgctggatg ctgcgcacaa agtgaaagat aaatgggcgc acacctcggt gcaggatcgc
     1561 gcggcgattc tgtttaagat tgccgatcga atggaacaaa acctcgagct gttagcgaca
     1621 gctgaaacct gggataacgg caaacccatt cgcgaaacca gtgctgcgga tgtaccgctg
     1681 gcgattgacc atttccgcta tttcgcctcg tgtattcggg cgcaggaagg tgggatcagt
     1741 gaagttgata gcgaaaccgt ggcctatcat ttccatgaac cgttaggcgt ggtggggcag
     1801 attatcccgt ggaacttccc gctgctgatg gcgagctgga aaatggctcc cgcgctggcg
     1861 gcgggcaact gtgtggtgct gaaacccgca cgtcttaccc cgctttctgt actgctgcta
     1921 atggaaattg tcggtgattt actgccgccg ggcgtggtga acgtggtcaa tggcgcaggt
     1981 ggggaaattg gcgaatatct ggcgacctcg aaacgcatcg ccaaagtggc gtttaccggc
     2041 tcaacggaag tgggccaaca aattatgcaa tacgcaacgc aaaacattat tccggtgacg
     2101 ctggagttgg gcggtaagtc gccaaatatc ttctttgctg atgtgatgga tgaagaagat
     2161 gcctttttcg ataaagcgct ggaaggcttt gcactgtttg cctttaacca gggcgaagtt
     2221 tgcacctgtc cgagtcgtgc cttagtgcag gagtcaatct acgaacgctt tatggaacgc
     2281 gccatccgcc gtgtcgaaag cattcgcagc ggtaacccgc tcgacagcgt gacgcaaatg
     2341 ggcgcgcagg tttctcacgg gcaactggaa accatcctca actacattga tatcggtaaa
     2401 aaagagggcg ctgacgtgct cacaggcggg cggcgcaagc tgctggaagg tgaactgaaa
     2461 gacggctact acctcgaacc gacgattctg tttggtcaga acaatatgcg ggtgttccag
     2521 gaggagattt ttggcccggt gctggcggtg accaccttca aaacgatgga agaagcgctg
     2581 gagctggcga acgatacgca atatggcctg ggcgcgggcg tctggagccg caacggtaat
     2641 ctggcctata agatggggcg cggcatacag gctgggcgcg tgtggaccaa ctgttatcac
     2701 gcttacccgg cacatgctgc gtttggtggc tacaaacaat caggtatcgg tcgcgaaacc
     2761 cacaagatga tgctggagca ttaccagcaa accaagtgcc tgctggtgag ctactcggat
     2821 aaaccgttgg ggctgttctg atataagaag ctggtcgcat tgggtattca ttgcctgatg
     2881 cgacgcttac gcgtcttatc atgcctacgg gaacctgacc gtaggccgga taaggcgtac
     2941 acgccgcatc cggcataaac aaagcgcacg ttgttacaat ttacgtttta aacctacgtg
     3001 cgcaataacc cagactacgc caacagaagt gcgtttctgg ggcgtgtttt gtcaggagtt
     3061 taatctcgcg tttcgatggt gagcgggcga accaggccgt aaatcaccat tggtgatccg
     3121 aggccaatca gcaataaacg gtagataatt gaaggtttaa tacgcttaat ctgtactgtg
     3181 gatgttttac tggagttatt cattagtttg gccaatagtt ccactatttc aatatgataa
     3241 acctcgtcct gttgaaaaac cagacagcac atttcagaaa gtatataaaa aacaatatgt
     3301 cgttatataa ataaggaaat tgcactgcat catggcattt cataatatgc gttaattgtt
     3361 cttttttata ttctttgagg gaaatatgtt cctggactat tttgcactgg gagtgcttat
     3421 ttttgtattt ctggtgatat tctatgggat tattatttta catgatattc cctacctgat
     3481 tgctaaaaaa cgtaatcatc ctcatgccga cgccatt
//
LOCUS       DAZSIO010000264         3511 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZSIO010000264 DAZSIO010000000
VERSION     DAZSIO010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.268"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..345
                     /locus_tag="ACHX5T_003983"
     CDS             <1..345
                     /locus_tag="ACHX5T_003983"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8468944.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
     gene            335..1129
                     /locus_tag="ACHX5T_003984"
     CDS             335..1129
                     /locus_tag="ACHX5T_003984"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8468945.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            1151..2575
                     /gene="patD"
                     /locus_tag="ACHX5T_003985"
     CDS             1151..2575
                     /gene="patD"
                     /locus_tag="ACHX5T_003985"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFR8468946.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            2785..>3511
                     /locus_tag="ACHX5T_003986"
     CDS             2785..>3511
                     /locus_tag="ACHX5T_003986"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFR8468947.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEE"
BASE COUNT          856 a          744 c          959 g          952 t
ORIGIN      
        1 atcctgcccg ttcaggcggc gcttgagcgt ttgccgccgt cattgttgca ggcgtcggct
       61 gatctcggcg cacgtccacg acaaaccttt cgctatgtgg tgctgccgct ggcaatcccg
      121 ggtattgccg ctggctctat ctttaccttc tcactcacac tgggcgattt tatcgtcccg
      181 cagctggttg gtcctccagg atattttatc ggcaatatgg tttattccca gcagggggcg
      241 attggcaata tgccgatggc ggcggcattc accctggtgc cgattattct catcgcactg
      301 tacctggcgt tcgtgaaacg tctgggagcg ttcgatgcac tctgaacgcg caccgttttt
      361 cctcaaactg gcggcctggg gcggcgttgt tttcctacat tttcccatcc tgataatcgc
      421 cgcctatgcg tttaacactg aagatgcggc gtttagtttt ccaccgcagg gcctgacgct
      481 gcgctggttt agcgtggcag cacagcgtag tgatattctt gatgccgtga cgctgtcact
      541 taaagtggcg gcgctggcga cattaattgc gctggtgtta gggacgctgg cagctgccgc
      601 gctgtggaga cgagactttt tcggcaaaaa cgccatttcg ctgttattgc tgctgcccat
      661 tgcgctgccg ggcattgtca ctggtctggc gctattaacc gcctttaaaa ccatcaatct
      721 ggagccggga tttttcacca tcgtggtcgg tcatgcgact ttttgcgtag tggtggtgtt
      781 taacaatgtc atcgcccgtt ttcgccgcac ctcgtggagt ctggttgagg cgtcaatgga
      841 tcttggggcc aatggctggc aaaccttccg ctacgtagtg ttgccgaatc tcagttcggc
      901 gttactggca ggaggaatgc tggcgtttgc cttgtcgttc gatgaaatca tcgttacgac
      961 ctttacggca ggtcatgaac gaacgttacc gttgtggttg ctcaatcagc ttgggcgacc
     1021 gcgtgatgta ccggtaacca acgtggtggc actgctggtt atgttggtaa caaccttgcc
     1081 gatcctgggg gcctggtggc taacccgcga aggcgacaat ggtcaataac cactgataca
     1141 ggaataagct atgcaacata agttactgat taacggagaa cgggttagcg gcgaagggga
     1201 aaaacagcct gtctataatc cggcaacggg ggacgtttta ctggagattg ccgaggtatc
     1261 cgcagagcag gtcgatgctg ctgtgcgtgc ggcagatgca gcatttgctg aatgggggca
     1321 aaccacaccg aaagcgcgtg cggaatgcct gctgaaactg gcggatgtta tcgaagaaaa
     1381 tggtcaggtt tttgccgaac tggaatcccg taattgtggc aaaccgctgc atagtgcgtt
     1441 caatgatgaa atcccggcga ttgtcgatgt ttttcgcttt ttcgcgggtg cggcgcgctg
     1501 tctgaatggt ctggcggcag gtgaatatct tgaaggtcat acttcgatga tccgtcgcga
     1561 tccgttgggg gtcgtggctt ctatcgcacc gtggaattat ccgctgatga tggccgcgtg
     1621 gaaacttgct ccggcgctgg cggcagggaa ctgcgtagtg cttaaaccat cagaaattac
     1681 cccgctgacc gcgttgaagt tggcagagct ggcgaaagat atcttcccgg caggcgtgat
     1741 taacatactg tttggcagag gcaaaacggt gggtgatccg ctgaccggtc atcccaaagt
     1801 gcggatggtg tcgctgacgg gctctatcgc caccggcgag cacatcatca gccataccgc
     1861 gtcgtccatt aagcgtactc atatggaact tggtggcaaa gcgccagtga ttgtttttga
     1921 tgatgcggat attgaagcag tggtcgaagg tgtacgtaca tttggctatt acaatgctgg
     1981 acaggattgt actgcggctt gtcggatcta cgcgcaaaaa ggcatttacg atacgctggt
     2041 ggaaaaactg ggtgctgcgg tggcaacgtt aaaatctggt gcgccggatg acgagtctac
     2101 ggagcttgga cctttaagct cgctggcgca tctcgaacgc gtcagcaagg cagtagaaga
     2161 ggcgaaagcg acagggcaca tcaaagtgat cactggcggt gaaaagcgca agggtaatgg
     2221 ctattactat gcgccgacgc tgctggctgg cgcattacag gacgatgcca tcgtgcaaaa
     2281 agaggtattt ggtccagtag tgagtgttac gcccttcgac aacgaagaac aggtggtgaa
     2341 ctgggcgaat gacagccagt acggacttgc atcttcggta tggacgaaag atgtgggcag
     2401 ggcgcatcgc gtcagcgcac ggctgcaata tggttgtacc tgggtcaata cccatttcat
     2461 gctggtaagt gaaatgccgc acggtgggca gaaactttct ggttacggca aggatatgtc
     2521 actttatggg ctggaggatt acaccgtcgt ccgccacgtc atggttaaac attaagttag
     2581 atgttagtgg ctgttatagg acggccacta tcgttttagg ttctgagttt aatggcggtt
     2641 aagtattaga atgattacat taataactgg cgtaaaaatt aggctaaatg tttgatggcg
     2701 atttttcatc ctgtcacagt tgattagcat aaggctttag agtatcgtta ttaaactaat
     2761 actttataag tagaagtatt aacaatgaaa gttcaattgt acaaattcac ggaagataaa
     2821 aataaaaccc ttaccttcag atggacgaaa aaacactttg agttttgtat ggataataaa
     2881 atctttttaa atcataaggg gaagaagtcg tataaagaaa ggaatttatt tcttttctca
     2941 aaaggtgaca agataacaat tgaagataat gttattgctg aagaatattc aacaatgcct
     3001 gtgaaaaatt tctccagtgt cggcgctttt tcatttccga cctgccattt ttcagggaat
     3061 atacgtatag gtcgattttg tagtattgca tcaaatgtaa aaataatggg tggaaatcat
     3121 cctttgaatc gttttactac tcacatgatg acttataacg gtgaatttga taagtttgct
     3181 atgagtgaat ttgaacgtag ctggacattg aaaccattca ttaccaaacc tgaaaatccg
     3241 attataggaa atgatgtctg gataggaaat gatgttgtat tgaaaggtgg catcgcaata
     3301 ggtgatggtg ctgttattgc tgccaattcc gttgttacca aagatgtgcc cccatatgcc
     3361 atagttgcag gagttcctgc aaaaataatc agatttaggt ttgattcaaa tgttattgat
     3421 gaactgttga gaataaaatg gtggaattat aactactcag atcttccgga taataataag
     3481 tgtgatgata taaattattt tgttgaagag a
//
LOCUS       DAZSIO010000265         3497 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZSIO010000265 DAZSIO010000000
VERSION     DAZSIO010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.269"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..311
                     /locus_tag="ACHX5T_003987"
     CDS             <1..311
                     /locus_tag="ACHX5T_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFR8468948.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="ACHX5T_003988"
     CDS             468..1217
                     /locus_tag="ACHX5T_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR8468949.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="ACHX5T_003989"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="ACHX5T_003989"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFR8468950.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="ACHX5T_003990"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="ACHX5T_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR8468951.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..>3497
                     /locus_tag="ACHX5T_003991"
     CDS             3361..>3497
                     /locus_tag="ACHX5T_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="YieH; catalyzes the dephosphorylation of
                     phosphoenolpyruvate, AMP and p-nitrophenyl phosphate;
                     purine and pyrimidine nucleotides are secondary
                     substrates; member of the haloacid dehalogenase-like
                     hydrolases superfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR8468952.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     R"
BASE COUNT          961 a          908 c          864 g          764 t
ORIGIN      
        1 cactggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agtctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcaccagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aacgttt
//
LOCUS       DAZSIO010000266         3456 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZSIO010000266 DAZSIO010000000
VERSION     DAZSIO010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.270"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(226..429)
                     /locus_tag="ACHX5T_003992"
     CDS             complement(226..429)
                     /locus_tag="ACHX5T_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468953.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="ACHX5T_003993"
     CDS             complement(431..586)
                     /locus_tag="ACHX5T_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR8468954.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="ACHX5T_003994"
     CDS             complement(789..1196)
                     /locus_tag="ACHX5T_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8468955.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="ACHX5T_003995"
     CDS             1273..1500
                     /locus_tag="ACHX5T_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR8468956.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="ACHX5T_003996"
     CDS             1484..1906
                     /locus_tag="ACHX5T_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR8468957.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1984..2761
                     /locus_tag="ACHX5T_003997"
                     /pseudo
     CDS             1984..2761
                     /locus_tag="ACHX5T_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2768..3456
                     /locus_tag="ACHX5T_003998"
                     /pseudo
     CDS             2768..3456
                     /locus_tag="ACHX5T_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          885 a          764 c          940 g          867 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagatttttg tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gctggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccctggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt
     1921 gccggattgt tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa
     1981 caaatggctt caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc
     2041 aggcttgctg agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc
     2101 tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt
     2161 acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag
     2221 gttggatggc tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac
     2281 ggaaaaagct ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg
     2341 aaacgaaaag gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa
     2401 gaggaagagg aagatctaaa tactgatctc cccctaaatc cccctcgcca aaaacgagcg
     2461 tctaaaaaat tcgagccgga ggctattgag ctgcccgatt ggttgccgga aacactctgg
     2521 catgagtggg tccggttcag acaggcattg cgaaaaccga ttcgaacgga gcagggcgat
     2581 acgggaactg gaaaaattcc gtcagcaggg ttttacacct gagcaggtga ttcgacacag
     2641 catcgccaat gaataccagg gcctgttcgc gccgaaaggt gttcggcctg agacgttgct
     2701 ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc ccgccaggct tcagggggta
     2761 acggaccatg aaaaatattg cgacaggcgg cgttctggaa cgcatccgcc gactgacccc
     2821 gcaacatgta accgccccat tcagaacggt agcggagtgg cgcgagtggc aacttgctga
     2881 aggccagaaa cgtagcgagg agatcaaccg cctgaatcgc cagttgcggg tggaaaaaat
     2941 tctgaatcgc tcaggcatcc agccgttgca ccgtaaatgc tcgtttgcga attaccaggt
     3001 gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa tccatcgccg atgaactgat
     3061 gaccgggtgt acaaattttg cgttcagcgg aaaacctgat accgggtaga atcacttagc
     3121 ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca gtgattgtgg ttaccgtggc
     3181 tgatgttatg agcgccctgc acgccagcta tgacgacggg cagtcaggcg aaaaattttt
     3241 gcgggagctg tgcgaagtgg atctgctggt tcttgatgaa attggcattc agcgcgagac
     3301 gaaaaacgag caggtggtgc tgcaccagat tgttgatcgc cggacagcgt cgatgcgcag
     3361 cgtggggatg ctgacaaacc tgaactatga ggtgatgaaa acattgctcg gcgagcgggt
     3421 gatggatcgc atggtcatga acggcgggcg ctgggt
//
LOCUS       DAZSIO010000267         3415 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZSIO010000267 DAZSIO010000000
VERSION     DAZSIO010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.271"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..239
                     /locus_tag="ACHX5T_003999"
     CDS             <1..239
                     /locus_tag="ACHX5T_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFR8468958.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            249..887
                     /locus_tag="ACHX5T_004000"
     CDS             249..887
                     /locus_tag="ACHX5T_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR8468959.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            890..2053
                     /locus_tag="ACHX5T_004001"
     CDS             890..2053
                     /locus_tag="ACHX5T_004001"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR8468960.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2137..>3415
                     /locus_tag="ACHX5T_004002"
     CDS             2137..>3415
                     /locus_tag="ACHX5T_004002"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR8468961.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEG"
BASE COUNT          760 a          804 c          987 g          864 t
ORIGIN      
        1 cattgttagg ttacgttatc ccattatcca gtgcggcaat caatgcggtg agtattccag
       61 actatttcgc ctggggcggg attgcactgg tgattcagtt actcgttttt gctggtgtca
      121 gactttatat gcccgcatta agcgaaaaaa ttattaatca caataccgca gcaggaatgt
      181 ttatgggaac cgccgcgctg gctggcggta tttttaacgc agcttgtatg acatggtaat
      241 ggacgatcat ggccagaaaa cgcaaatcaa gaaataacag taaaattggt cacggagcga
      301 tcagtcgcat tggtagaccg aataatcctt ttgaaccgcg tcgcaatcgc tacgcacaaa
      361 aatacttaac gttggcgcta atgggcggtg ccgctttttt cgtattgaaa ggttgtagcg
      421 atagtagcga tgtcgataac gacggcgacg gaacatttta cgcgacagtg caggattgta
      481 ttgatgacgg taataatgcc gacatttgcg cgcgtggctg gaacaacgcc aaagcggcat
      541 tttatgccga tgttccaaag aatatgactc agcagaactg tcagtctaag tacgaaaatt
      601 gctactacga caatgttgaa cagagttgga tcccggtcgt ttctggattt ttgttaagcc
      661 gggttattcg taaagatcgc gatgagccgt tcgtctataa cagcggcgga tcctcatttg
      721 cttcccgccc cgtctggcgc aacacttccg gtgattactc ctggcgcttc ggttctggca
      781 aaaaagagtc ttactcttca ggcggcttta ccaccagaaa agcgtcgacc gtttcgcgcg
      841 gcggctatgg tcgttcttcc agcgcccgtg ggcattgggg aggctaatca tgctgagaca
      901 caacgttcct gtgcggcggg atctggacca gatcgccgct aacaacggtt tcgactttca
      961 tatcatcgac aatgaaatct attgggacga gagtcgggct taccgtttta ctctgcgcca
     1021 gattgaagag cagatcgaaa aaccgactgc ggaactgcat cagatgtgcc ttgaggtggt
     1081 ggatcgcgcg gttaaagacg aagagatcct gacgcaactg gcgatcccgc cgttgtactg
     1141 ggatgtgatc gccgaaagct ggcgcgcacg cgatccttcg ctgtatggcc ggatggattt
     1201 tgcctggtgt ggcaatgcgc cggtgaagct gctggagtac aacgccgata cgccgacctc
     1261 gttgtatgag tcggcttatt tccagtggtt gtggctggag gatgcccggc gcagcggcat
     1321 tattccgcgt gatgccgatc agtacaacgc cattcaggaa cgcctgattt cgcgctttag
     1381 tgagctttac agtcgggaac tgttttattt ttgctgctgt caggacaccg atgaagacag
     1441 gagtaccgtg ctgtacttgc aggactgcgc ccagcaggca gggcaggagt cgcggtttat
     1501 ctacattgaa gatctcggtt tgggcgtcgg cggcgtactg accgatcttg atgataatgt
     1561 catccagcgt gcatttaagc tgtatccgct ggagtggatg atgcgtgacg ataacggtcc
     1621 gctgctgcgc aagcgtcgcg agcaatgggt ggagccgtta tggaaaagta tcttgagtaa
     1681 taaagggcta atgccgctgc tttggcgctt cttccctggt catcctaacc ttcttgcatc
     1741 ctggtttgag ggtgaaaaat cgcagattgc cgctggcgaa agctatgtgc gtaaaccgat
     1801 ctactcgcgc gaaggcggaa acgtcaccat tttcgacggt cagaataacg tcgttgacca
     1861 cgctgatggt gattacgccg atgaaccgat gatctaccag gcgtttcaac ctctgccgcg
     1921 atttggcgat agctacacac tcatcggtag ctggattgtc gatgatgaag cgtgcggaat
     1981 ggggatccgt gaagataaca cactgatcac caaagacacc tcacgtttcg ttccgcatta
     2041 cattgctgga taagaatgtt ttagcaatct ctttctgtca tgaatccatg gcagtgacca
     2101 tactaatggt gactgccatt gatggaggga gacacagtgc actggcaaac tcacaccatt
     2161 tttaatcaac ctataccatt aaataacagc aacttatacc tgtctgatgg cgcgctttgc
     2221 gaagcggtaa cgcgtgaagg tgctggctgg gatagcgatt ttctagccag tattggtcag
     2281 cagttaggaa cggctgaatc ccttgaactg gggcggctgg cgaatgtaaa cccgcctgaa
     2341 ctgttgcgct acgatgccca ggggcggcgt ctggacgatg ttcgtttcca tcccgcatgg
     2401 cacctgctga tgcaggcgtt atgtaccaat cgggtgcaca atcttgcctg ggaagaagac
     2461 gctcgctccg gcgcatttgt ggcgcgcgcg gcgcgtttta tgttacatgc gcaggttgag
     2521 gctgggtcgt tatgtccagt aacgatgacc tttgccgcca cgccattgtt gttacagatg
     2581 ttacccacgc cgtttcagga ctggaccacg ccgctgctga gcgatcgcta cgattctcac
     2641 ttattgccag gtgggcaaaa acgcggtttg ttgattggca tgggaatgac ggaaaagcag
     2701 ggcggttccg atgtcatgag caacaccacc cgtgcagagc gtttggaaga tggctcttat
     2761 cggctggtgg ggcataaatg gtttttctcg gtgccgcaaa gcgatgcgca tctggtgctg
     2821 gcgcagaccg cgggcggtct gtcctgcttt tttgtgccgc gctttttgcc tgacgggcaa
     2881 cgcaacgcga ttcgcctcga gcggctgaaa gataagctgg gtaatcgctc taacgccagt
     2941 tgcgaagtgg agtttcagga tgccattggc tggttattgg ggcaggaagg ggaaggaatt
     3001 cgtctgatcc tgaaaatggg ggggatgacg cgttttgatt gcgccctggg tagccatgcc
     3061 atgatgcgcc gtgcattttc gctggcgatt tatcatgcac atcaacgcca tgtttttggt
     3121 aatccattga tccaacagcc ccttatgcgt catgtcttaa gtcgcatggc acttcagctt
     3181 gaagggcaaa cggcgttgct gtttcgtctt gcgcgagcgt gggaccggcg tgccgatgcc
     3241 aaagaagccc tgtgggcgcg tttatttacg cctgcggcaa aatttgtgat ctgcaaacga
     3301 ggtatgccgt ttgtggccga agcgatggag gtgctgggcg gcattggtta ttgcgaggag
     3361 agcgagctgc cgcggcttta ccgggagatg ccggtaaaca gtatttggga aggtt
//
LOCUS       DAZSIO010000268         3413 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZSIO010000268 DAZSIO010000000
VERSION     DAZSIO010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.272"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            75..791
                     /gene="nanC"
                     /locus_tag="ACHX5T_004003"
     CDS             75..791
                     /gene="nanC"
                     /locus_tag="ACHX5T_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR8468962.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            811..1917
                     /gene="nanM"
                     /locus_tag="ACHX5T_004004"
     CDS             811..1917
                     /gene="nanM"
                     /locus_tag="ACHX5T_004004"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR8468963.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1982..2962
                     /locus_tag="ACHX5T_004005"
     CDS             1982..2962
                     /locus_tag="ACHX5T_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR8468964.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3072..3277
                     /locus_tag="ACHX5T_004006"
                     /pseudo
     CDS             3072..3277
                     /locus_tag="ACHX5T_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT          995 a          726 c          801 g          891 t
ORIGIN      
        1 attacacaac ataaatgcta attgtttatg cgggctttgt attgctttct gtatcctaca
       61 aatgagtgaa atttatgaaa aaggctaaaa tactttctgg cgtattatta ctgtgctttt
      121 cgtccccatt aatttctcag gctgcgacac tggacgtacg tggtggatat cgtagtggaa
      181 gccacgccta tgagactcga ctcaaagtca gtgagggatg gcaaaatgga tggtgggcaa
      241 gcatggaaag taatacctgg aataccattc atgataataa aaaggaaaat gccgcactca
      301 atgatgttca ggttgaagtt aattacgcaa ttaaacttga tgatcaatgg acggtgcgcc
      361 cgggaatgtt aacgcatttt agcagcaacg gcacacgcta cggaccctac gtaaaactgt
      421 cctgggacgc gacaaaagat cttaattttg gcattcgcta tcgttacgac tggaaagctt
      481 accgacaaca agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt tgggatggat
      541 atgtcactta ccatattaat agtgatttca ccttcgcatg gcaaacgacg ctatacagca
      601 aacagaatga ttatcgctat gcaaaccaca agaaatgggc gacggaaaat gcctttgttc
      661 tacaatacca tatgacgccc gatattacgc catacataga atatgactac cttgaccgcc
      721 agggtgttta caacggcaga gataatttat cggaaaacag ttatcgcatt ggtgtgtcat
      781 ttaaactgta gtagacagga gacagtcaca atgaataaaa ctataatggc gcttgctatc
      841 atgatggctt catttgccgc aaacgcgtct gtattaccgg aaactcctgt gccatttaaa
      901 agtggtaccg gagcaattga taacgacact gtctacattg gtttaggtag cgcaggtaca
      961 gcatggtaca agctagatac acaggccaaa gataaaaaat ggactgcgtt agctgcattc
     1021 cctggtggac cgagagagca agcaacctct gcatttattg atggcaatct gtatgtgttt
     1081 ggcggcattg gcaaaaacag cgagggattg actcaggtat ttaatgacgt acacaaatac
     1141 aaccccaaaa ccaatagctg ggttaaattg atgtcgcacg cgccgatggg catggcgggt
     1201 catgtgactt ttgtacacaa cggcaaggct tatgttactg gcggtgttaa ccagaatatc
     1261 ttcaatggct attttgaaga tctcaacgag gctggaaaag attcaaccgc tatagataaa
     1321 atcaacgccc actattttga caaaaaagca gaagattatt tcttcaataa gtttctgttg
     1381 tcttttgatc cctcaacaca gcaatggagt tacgctggcg aatcgccctg gtacggaacg
     1441 gctggtgcgg cggttgtgaa taaaggtgat aaaacctggc ttattaatgg cgaagccaaa
     1501 ccaggattgc gaacggatgc cgtatttgaa cttgatttca ccggtaataa tttaaaatgg
     1561 aataagcttg atcccgtctc atcaccagat ggcgtcgctg gcggttttgc ggggataagc
     1621 aatgattctc ttatatttgc tggaggggcc ggattcaaag gttcacgaga aaattaccag
     1681 aacggtaaga actatgcgca tgaaggcctg aaaaaatcat atagcactga tattcatctt
     1741 tggcataacg ggaaatggga taaatcgggt gaattatcgc aaggtcgggc ctacggagta
     1801 tcattgccct ggaataatag tttattaatt attggcggtg aaactgcagg cggcaaagcg
     1861 gtgacggatt cagttttaat ttctgtgaag gataataaag tcacagtaca aaactaacgc
     1921 ttcagggccc cggtaaaggg gccctgctat caatttgtca tttgtattac gcgaaaatta
     1981 tatgaacgca ataatatcac ccgattatta ctatgttctt accgttgctg gtcagtctaa
     2041 tgccatggcg tatggcgaag gactgccatt accggacagg gaagatgcgc ctcattccag
     2101 aattaaacaa ttagcgagat ttgcgcatac gcatcccgga ggcccgccat gtcactttaa
     2161 cgacattatt ccactgactc actgcccaca cgatgttcag gatatgcagg gttatcacca
     2221 tcctctggca acgaatcatc aaacacagta cggcaccgtt ggccaggcac tgcatattgc
     2281 acggaaatta ctgcccttca ttcctgataa tgcagggatt cttatcgttc cgtgttgccg
     2341 tggcggatcg gcttttaccg cgggcagcga agggacatat tcagaacggc acggagccag
     2401 ccatgatgct tgtcgttggg gaacggatac tccgctgtac caggatttag tcagcagaac
     2461 gcgagccgca ctggcaaaaa atccgcagaa caaattcctc ggcgtatgct ggatgcaagg
     2521 cgaatttgac ttaatgacca gtgactacgc gtcacaccct caacacttta atcatatggt
     2581 tgaagccttt cgtagggatc taaaacaata ccattctcag cttaataata ttactgacgc
     2641 gccgtggttt tgcggcgata ccacctggta ctggaaagaa aatttcccgc attcgtatga
     2701 agctatttat ggcaattatc aaaataatgt tttagccaat attattttcg tcgacttcca
     2761 gcaacaaggt gaaagaggac tgacgaacgc gcctgatgaa gacccggacg atttaagcac
     2821 gggatattac ggttcagcgt accggtcacc ggagaactgg acgacggcac tgcgaagcag
     2881 tcatttcagc acggcagccc gtcgggggat tatttctgac agatttgtag aagcaatttt
     2941 gcagttttgg cgcgaaaggt gagcggggag tttattcatg ggggatgtcc ttgttcgttc
     3001 tcagagtaga caacaaaaaa caaatgagat caaggtaata tctcttcact tgcgggctcg
     3061 aacagcaggc aattgccaat ataataatct ggcacaaggg tgaaaaacgc gcaccgcata
     3121 aaccgttgtt attgcttaca gattagccgg atgccttaat agacatccgc gccttttcga
     3181 ttatggctcg gaaatctacg aacatctaca cagtttactg gaacgttttg ggccacagcg
     3241 ttcgcagcac cgtcctgata tgccgttctg gcgattataa gtcgacggat tctggcaaat
     3301 agacgatgat gatatgctta atgatgttca aagaaattag gccattatta tattattgct
     3361 tgcattttct ttcattccta aaagcacccg ccattaatcc caggcgcatt atg
//
LOCUS       DAZSIO010000269         3373 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZSIO010000269 DAZSIO010000000
VERSION     DAZSIO010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.273"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..1202
                     /locus_tag="ACHX5T_004007"
     CDS             <1..1202
                     /locus_tag="ACHX5T_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFR8468965.1"
                     /translation="TKDGQRLRNMIMSFNADIVARSPKKKPTFFPEQIEGYEYMYGGN
                     DDYPYYLQNRTDENGNDLPIGPISYMENPEVPQANAYMLEAATNAVKEVASLGVDAQA
                     ANGQVAFDTVNQLNMRADLETYVFQDNLATAMRRDGEIYASMVNDIYDVPRHVTLTLE
                     DGSEKDVQLYAQVVDYQSGNVVTLNDIRGRYECYTDVGPSFQSMKEQNRAEIQELLTK
                     VPQGTPEFQMLMLQYFTLLDGKGVEMMREYANKQLVMMGLKKPETPEEMEMVQQAQQQ
                     PQQPSAEQIQAQGILLQGQAELLKAENQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMD
                     LDKQAELREYLRLVGQFQQQRSKDARANAELLLKDADQTHSQRMDFANLMRQVQIPSG
                     GVAETPQ"
     gene            1216..2127
                     /locus_tag="ACHX5T_004008"
     CDS             1216..2127
                     /locus_tag="ACHX5T_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HFR8468966.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            2127..>3373
                     /locus_tag="ACHX5T_004009"
     CDS             2127..>3373
                     /locus_tag="ACHX5T_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFR8468967.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACA"
BASE COUNT          927 a          872 c          872 g          702 t
ORIGIN      
        1 tgacgaaaga cggtcaacgc cttcgtaaca tgattatgtc gttcaacgcc gatattgttg
       61 ctcggtcacc gaagaagaaa ccgaccttct tccctgagca aatcgaaggc tacgaataca
      121 tgtacggcgg aaatgatgac tatccgtact atctgcagaa caggactgat gaaaacggta
      181 acgacctgcc gattggtcca atctcctaca tggaaaaccc tgaagtgccg caagccaacg
      241 cttacatgct tgaggctgcc accaacgcag tgaaagaagt ggctagtctt ggcgtggatg
      301 cgcaggcggc aaatggtcag gtcgcttttg ataccgtcaa tcaactgaac atgcgggcag
      361 accttgagac atacgtgttt caggataacc tggctaccgc aatgcgacgt gatggcgaga
      421 tttatgcctc aatggtcaac gatatttatg acgttcctcg tcatgtaacg ctgacacttg
      481 aagatggaag cgagaaagac gttcaactct atgcgcaagt tgtcgattac cagtccggca
      541 atgtggtcac actcaacgac attcgcggtc gatatgagtg ctatacagac gttgggccat
      601 ccttccagag tatgaaggaa cagaaccgcg cagagattca ggagttgctc accaaggttc
      661 cgcaaggtac tccagagttc cagatgctga tgctgcaata cttcacgctg cttgacggta
      721 aaggcgtcga gatgatgcga gagtacgcga acaagcaact ggtgatgatg gggctgaaga
      781 aaccagaaac acctgaagag atggagatgg tgcagcaggc acaacaacag ccgcagcagc
      841 catcagcaga gcaaattcag gcgcagggta tccttctgca aggtcaggct gaattgctca
      901 aggcagagaa ccaacaggcg cagattcagg ttgaagctgc caaggttgaa gcccaaaacc
      961 aactcaacgc cgcgaagatt gcagaaatat tcaacaatat ggacctcgac aagcaggcag
     1021 aactgcgtga gtacctcagg ctcgtaggtc aattccagca acagcgcagc aaagatgctc
     1081 gtgctaacgc tgagctgctt cttaaagatg cagaccagac tcattcacaa cgcatggatt
     1141 tcgcgaatct tatgcgtcaa gttcaaatcc cctccggcgg agtagccgag acacctcaat
     1201 aagagagagt taatcatgga ccaaaccacc gacattcagg cttctgaaga attaaccctg
     1261 cccggcaatc atgcagcggc atctgctgat ggcttagttg tcgataatgc caacgacagc
     1321 gcaggtcagg aagaaggctt tgagattgtc cggaaagacg atgagaaacc aaaacaagac
     1381 ccggcaacta atgctgaatt tgcccgtcgc cgcatcgaac gcaaacgcca gcgtgagctt
     1441 gagcagcaga tggaagcggt taagcgtgga gagttgccgg agcacctgcg ggtgaaccct
     1501 gagttaccaa aacaaccaga ccctaacgat tatctttccg aagatgcact ggctaagtac
     1561 gactatgacc agagccgcgc actggctgcc ttccagcagg caaacagtga atggcagatc
     1621 aaggctatgg acgcacgaag ccaggctgtc gccgagcagg gtcgcaaaac tcaggagttc
     1681 acccagcaat cagcgcaata cgtcgaggcg gcccgtaagc actacgaggc agcggaaaag
     1741 ctcaatatcc ctgactatca ggagaaagag gatgcattca tgcaactggt gccgccagca
     1801 gtcggtgccg acatcatgcg cctcttcccg gagaaatccg ccgctctcat gtatcacctt
     1861 ggtgctaatc ctgagaaaac acgccagttg ctggcgatgg acgggcaatc cgcgctgatt
     1921 gaactcactc gactgtcaga acgtttaact ctcaagcctc gagccaagcc tgtttcagaa
     1981 gccccgttac ctgatgaacc cattcaggga catgctgttg ctgcaaatat ctctgcgatt
     2041 gaaaagcaga tggaggcggc agcaaacaaa ggggatgtag agacgtaccg caagctcaag
     2101 gcgcaactga ataaaggaat tcgataatgg cattaaatga aggtcaactg gtcacgtatg
     2161 ctctggatga aatcatcgga accgtccaga acctgacgcc aatggcgtca aaagtgacaa
     2221 aatacccccc tccggcagaa tccatgcagc gttcaagcaa caccgtgtgg atgcctgttg
     2281 agcaggaagc gccaacccag actggctggg atttaactgg caacgcaacc gggattctgg
     2341 aactctccgt gaagtgcaac atgggcgatc cggataacga tttcttcgag cttcgtgcag
     2401 atgacctgcg tgatgagcgt tcttaccgtc gccgcatcca ggcatccgcc aaaaaactgg
     2461 cgaataacat tgagtcagcg attgccaaac aggcaactga aatgggctcg cttgttgttc
     2521 acgatacccg cgcaattggt ccatcgactg gcctgcctgg ctgggatttt gtgtctgatg
     2581 cagagcgact gatgttctcc cgtgagctaa accgcgacat gggcatcagt tacttcctga
     2641 accctgacga ttaccgcaaa gcaggccgca acctggtaga tggtgacatc ttcgggcgcg
     2701 ttcctgaaga agcatatcga aacggtacta ttcagcgtca gattgctggc tttgatgaaa
     2761 ttcttcgctc accgaaactt ccggcagtta ccaagtcaac cgcgactggt gtaactgttt
     2821 ctggtgcgca gaagtttaag ccgcaggcat acacccttga taccgatggt aacaaagaga
     2881 acgtcgacaa ccgtgttgca acggtgaccg tatcctccac caccggattt aagcgcggcg
     2941 acaaaatcag cttcattggt gtgaagttcc tgtctcagat ggcgaagaat gtgctgactg
     3001 atgacgcgac tttctcaatc acccgtgtga tcgatggtac tcacatcgaa atcacgccga
     3061 agccgattgc gctggatgac gcgtcactga caaaagaaga gaaggcttac gctaacgtaa
     3121 acacctctct tgctgatacc actccggtaa acgttctgaa cgtggcaaca accaccgcta
     3181 acgtgttctg ggctgatgac tcaatccgcc tgctgtctca gccgatcccg gtaacccatg
     3241 aactgtttgc tggcatgaaa acgtcttcct tcagcattcc aggtattggt gttaacggca
     3301 tcttcgcaac gcagggtgat atcaacactc tgtctggtaa gtgccgtatt gctgtgtggt
     3361 attcagcatg tgc
//
LOCUS       DAZSIO010000270         3348 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZSIO010000270 DAZSIO010000000
VERSION     DAZSIO010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.274"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            19..897
                     /locus_tag="ACHX5T_004010"
     CDS             19..897
                     /locus_tag="ACHX5T_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR8468968.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            894..2285
                     /locus_tag="ACHX5T_004011"
     CDS             894..2285
                     /locus_tag="ACHX5T_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR8468969.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2282..2539
                     /locus_tag="ACHX5T_004012"
     CDS             2282..2539
                     /locus_tag="ACHX5T_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468970.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2634..2834
                     /locus_tag="ACHX5T_004013"
     CDS             2634..2834
                     /locus_tag="ACHX5T_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFR8468971.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2827..3207
                     /locus_tag="ACHX5T_004014"
     CDS             2827..3207
                     /locus_tag="ACHX5T_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR8468972.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          885 a          726 c          969 g          768 t
ORIGIN      
        1 cattctgagg tgacagcgat gatgacgttt aacctgcgtg aacaacaaaa aagactacag
       61 gcgcgaatgg atgagttacg ggcagagatt gcatttgctc agaagggcga aaagccatgg
      121 ccttatcgtt cctgcctgat gcgtgaaggt cgcggatatt gcgaaaaaca tggcgaatat
      181 cacacgcata tactggtgtg gagcgatcgt aatggcgagg acagagaaga aatttcatgc
      241 tgccctgact gcttaatcgc tgaggccaac gatttgacca tggagctgtc gtccatcaag
      301 gcggaagagc tgactgataa cgccggaatt gccctgcgtt ttcgggactg cgagtttgat
      361 aattatctgg aggttaatcc tggcgcagcc agaaatcttg cggcctgtcg ccgctatgcg
      421 gagaactggc cagatatgct ggagaacggt accagtcttg ttatgaccgg cagttgtggt
      481 accggaaaaa atcatctggc tgtggctatg gcaaaacaca tcatccgtaa ctatctggcc
      541 agtgtggaga tcaccgacgt gatgcgcctt acccgtgctg tgaaaaattg ctggcggaat
      601 gacagcgaaa aaacagcgga tgaagttatt gagcgttatg cgtcaatgga tttgctgatc
      661 atcgacgaag ttggcgttca gtttggcagc gcggctgaaa tggctatttt gcaggaaatt
      721 atcaacgcca ggtacgaaag tatcttgccc accattctga tcagtaacct ctcaccggaa
      781 gagttgtggg cgttcatcag tccccgaatt gccgacagga tcacagacgg gggacgcaac
      841 tggttgtcgt ttaactggcc cagttaccgt tctcgtatca gaggtgtggc tgcatgacaa
      901 ctccagtatg gcgtaacgat gaccttgaag gcgctgtcat cggcgcgttc tttctgcgcg
      961 gagcagatcc tgaagtgatg gatattctgg ccacactgcc agcggacgtt ttttctgtac
     1021 gagcgtatca ggatatctat acaggcatct gcagacaggc ccgtgtttca ggagtgattg
     1081 accccgtgct gttgtgtaat gagatgccgg aacttgcccc ggtgattact gataccgggc
     1141 gtaaaacctg ggtgaagtct tcactggagc actatgttgc agcgttgcgg cgcaatgccg
     1201 cactgcgcga tgcagaaaaa acactgaatg aggcgctgca gaaattacgt gatgcgcata
     1261 cctgtgaagc agctgaagat gccctgaagg atgcgcagaa catgatggtc acactgtcga
     1321 caggaaaggg cgtcattcag ccggtacata ttgatgatgt gcttccggaa gtggttgagc
     1381 gtgttgaatg ccggaatcag gggctggaga aatccaggac gttgatgacc ggtattgatg
     1441 aactggacgc aaaaacaggc ggtatggagc ccggagacct ggtattcatt gccgcccgtc
     1501 cttcgatggg gaaaaccgaa cttgcgctgg acatcatcga caaggtgact gagcaggggc
     1561 atggtgtgct tctgttcacc atggagatgg cgaacatcca gattggtgaa cgtatggtgt
     1621 ctgcggctgg agggatgcca gtatcacgcc tgaaatctgt ggctcacttt gaagatgaag
     1681 actggacgcg tttctcacaa ggggtggggc ggatgaccgg gcgcaatatc tggatggtgg
     1741 accaggcgaa cctggccatt gatgagatat gcgcaacaac gaaacaccac ctgattaaat
     1801 atccggaaac ggcgctggtg gtggttgatt atctcgggct gataaaaacc cgaaccacgg
     1861 ggcgtcatga ccttgccgtg ggtgaaatct caaaggggct taaaggcctg gcaaaatccg
     1921 gtggttttcc gttgattgcg ctgagccagc tctcccgcgg tgtggagtcc agacccaata
     1981 aacgtcccat gaactcagac ctgaaaaatt ccggagaaat agaggcggat gcagacatca
     2041 ttctgatgct ttacagggat gaagtgtaca acccggatac gcaggccagg ggcatcgcag
     2101 aaatcaatat cacgaaacaa cgtaacggtt ctctgggaac gatttaccgg cgtttttata
     2161 acggacattt tctgcctgtg gaccaggaaa gtgcacgggt gctttccact cccatgaagc
     2221 ccggcaatcc gcgcagatac agtaataaac gaactgacag cagtaagatg gagcgtttct
     2281 tttgaacaac cagacaatga cttttacccc tgaacaattg cgtaaacacg cgcaggaaat
     2341 gctgcgtcat gctgaacaac tcgaaaaaac gggcataaca aaagatgcta tccgtaaaga
     2401 tatggtaccc gcgcttcggg aactgatgca ggcgaagcat cgcgcacaga aagcagtaga
     2461 tgagctggtg gactgtgtgg cagagctgga aaccagagtc ggaaagtttg aaaaactggt
     2521 gcaggaggcg ctgcgctgat gcgccatgag tttgttttgc cctggcttgt gcgcgatcat
     2581 tatgtccctg gtggaaagcc cgggataaaa atcaccgaac cggagagtat ttgatgagcg
     2641 tcaaaattca aacgataccg gaactgctta tccagacacg aggtaatatg accgaagttt
     2701 cacgaatgct gaactgtaat cgcgccacgg taagaaaata tgctgaagat aaagagggca
     2761 aagggcacgc cattgttgat ggtgttctga ttgttcatcg tggatgggac aggggtaaag
     2821 acagtgatgc gtgatattca gcaggttctt gagcgctggg gcgcgtgggt ggctaataat
     2881 catgaggatg tgacctggtc gtccattgct gccggtttta agggattaat cccttcaaaa
     2941 gtaaaatccc gcccgcaatg ctgtgacgat gacgcgatgg ttatatgtgg gtgtatggcc
     3001 cgccttaacc ggaacaacaa cgatctacat gacttgctgg ttgactacta cgtgtttggg
     3061 atgacattca tgacgctggc gcggaagcat ggatgctcag atggttatat aggaaagaag
     3121 ctacaaaaag ccgagggggt ggtggaagga atgttgatga tgttgggtgt ccggttagaa
     3181 atggacaaat atattgaaca tccctgatgg taaggggcga ctttctagaa aatattttac
     3241 gtacgtaaaa aacgggatat tctgttaaga gtggttgctt cgccacacag cttaaacccg
     3301 cctccgggcg ggttttttat gcctgaaaaa cggtacagga cgttaaac
//
LOCUS       DAZSIO010000271         3341 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZSIO010000271 DAZSIO010000000
VERSION     DAZSIO010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.275"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(110..1291)
                     /locus_tag="ACHX5T_004015"
     CDS             complement(110..1291)
                     /locus_tag="ACHX5T_004015"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR8468973.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            1546..2775
                     /locus_tag="ACHX5T_004016"
                     /pseudo
     CDS             1546..2775
                     /locus_tag="ACHX5T_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            3202..>3341
                     /locus_tag="ACHX5T_004017"
     CDS             3202..>3341
                     /locus_tag="ACHX5T_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8468974.1"
                     /translation="MQLYTDREVLNMGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCA
                     NS"
BASE COUNT          906 a          805 c          692 g          938 t
ORIGIN      
        1 tgagaatgga aaaatgaggg agcccagaaa atgtatgtcg acttgccagc catcattatt
       61 caacagatta atgacggcag atatttatcg gtaaggagga aatgcagagc tactcgtcac
      121 gttcaatagt caatgccacg ccctggcccc cgccgataca aagcgttgcc agccctttgc
      181 gggcattacg tttcaccatt tcatgaacca gagaaaccag gattcggcaa ccggaagcgc
      241 ctatcgggtg accgagtgcg atcgcgccac cattgacatt gacccgacgc tcatcccact
      301 caagcatctt gccaaccgaa agcgcctgtg cagcaaacgc ttcattagcc tcgataagat
      361 cgacatcagc caactgccag cctacacgct ccaggcaacg gcgggtcgca tacaccggcg
      421 caattcccat caatgccgga tctacaccaa cgctggcaaa tgcgcggatg cgggccagca
      481 cgggtaaatt caacgctcgt gctttggctt cgctcatcat cattaccgca gctgcgccat
      541 cgtttatgga tgatgcattc cccgcggtca cagaaccgag actatcaaat gaaggattta
      601 aacgggctaa accttctgca ctggcgtcag tgcgtggctg ttcatcggta tcaacaacca
      661 acgtctgacc gttactttgg atcattaccg ggacgatctc atctttaaat cgtccggcgt
      721 caatcgccgc tcgcgctttt tgttgcgagc taagtgcgta agcatcctgc aactgacggc
      781 tgatgccata ttcgcgagcc agattttcgg cggtgacacc aatatgataa tcattgaagg
      841 catcccacaa cccatcatgc accagactgt caaccaactg gctattgcca agctgttcac
      901 cggtgcggct atcagtcaga acatgtggtg cgcggctcat gttttcctgg ccacctgcga
      961 tgacaatatc agcctcgcca cactgtatcg cctgagtagc cagatgcagt gctttaagac
     1021 cggaaccgca aacgtcatta atcgtgattg cagaaacgct gttaggaaga ccacctttaa
     1081 tagccgattg ccttgccgga ttctgccctg cccctgcagt caacacctga ccaagaatta
     1141 cttcatccac cgcatatgca ggaacgccgg tacgttctat taacgctttc acgaccagac
     1201 taccaagttc cacggcggaa tgacccgcta acgcaccacg aaagcagccg ataggtgtcc
     1261 gtaacgcccc gacaatcaca acgtctttca tcacaacctc gcaccaatta acagtgcaat
     1321 agtaaatgat tgttaattac agttatctta attgtttaaa aaaagtgatt tttatcacaa
     1381 aggaaatatg cctgagcagt agtcagagtc ataactggca cgtaaggttt gcaaccacta
     1441 acccaccaat agatgggtag atagggcgtt aatctcccat acttaacttg gtttatggta
     1501 aattgccctc cattttgttt aatttgtaga tgatacgttc agataatgtc taatatttgg
     1561 tctaaagaag aaactctgtg aagtttcgcg ctctatggca cagccgttga tgcaggcacg
     1621 ctattccttc ctattcagtt aggttcggca ggggctgtgg tcctgtttat tactgctctg
     1681 gtcgcctggc ctttaacata ttggccacat aaagccttat gccagttcat cctctcatca
     1741 aaaacatcag caggtgaagg gataacgggc gcggtaacac actactatgg caagaagatt
     1801 ggtaatctga ttaccacgct gtacttcatc gccttttttg tcgtcgtgtt gatatatgca
     1861 gtggcaatta ccaactcact tacggaacag ctggcaaagc atatggttat tgatcttcgc
     1921 atccgtatgt tggtgagtct gggtgttgta ttaattctga atctcatttt tctgatggga
     1981 cgtcatgcca ctattcgggt aatgggattt ttggtattcc cattgattgc ctatttctta
     2041 tttctttcca tttaccttgt cggtagttgg caacctgatc tattaacaac ccaggtagag
     2101 ttcaatcaga atacccttca ccagatatgg atatcgattc ccgtgatggt tttcgccttt
     2161 agccatacgc ccattatttc tacgtttgcc atagacagac gtgaaaaata tggcgaacac
     2221 gctatggata aatgcaaaaa aattatgaaa gtcgcttatc tcatcatctg cataagtgta
     2281 ctgttctttg tctttagctg cctgctttct attccatctt cgtatattga agcggctaaa
     2341 gaagaagggg tcaccatttt atcggcgctt tctatgctgc cgaacgcccc agcatggttg
     2401 tcaatttccg ggattattgt cgcagtagtt gcgatgtcga aatcattcct gggtacgtac
     2461 tttggcgtta ttgaaggtgc cacagaggtc gtcaaaacaa cactacagca ggttggtgta
     2521 aagaaaagtc gtgcatttaa ccacgcacta tcaattatgt tggtatcgct gattaccttc
     2581 attgtttgtt gcattaaccc gaacgcgatt tcgatgattt acgcgatcag cggcccgctc
     2641 attgccatga tacttttcat catgcctacg ctgtcaacgt atctcatccc ggcgcttaaa
     2701 ccctggcgtt ccatcggaaa tctgattacg ctgatcgtgg gtatcctgtg cgtatcggta
     2761 atgttcttta gctaatatcc gttgtggatg gttcaaaatt gctacaattt tgaaccattc
     2821 catttttcat aatattcatt tgttattatt ccgccctatc acttctctcg tcatatccta
     2881 gaattatcag ttagttaaac acacaaagag atctgaacat cttaaataag actatttaag
     2941 atgcataact tagattcgca agatatcttc tggtcattca aaaacaattt gcgatataga
     3001 tattctcata ccatgagata gttatctgga ccacgttcga ccatgcaaga ttaaatcagt
     3061 gaaacataat catatttaat aacgagaaat gcatttttaa atatcttaat cagaattata
     3121 gcaaatacag attaatcgca atttttccca gcaaaattac aaaatcaagg tgtattgaca
     3181 tcatcaatca ccaatgaaat tatgcaatta tatacggata gggaggttct taacatgggg
     3241 ctttgtagtc gttataaaag tcttacatgc aatagttgct caatgcattg ccaaataatg
     3301 ccagaagagt caccgcgttt gcagtactgc gcaaactcgt g
//
LOCUS       DAZSIO010000272         3325 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZSIO010000272 DAZSIO010000000
VERSION     DAZSIO010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.276"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..208
                     /locus_tag="ACHX5T_004018"
     CDS             <1..208
                     /locus_tag="ACHX5T_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468975.1"
                     /translation="RHPDKRFIFNGNEIWKRGESTTEYTRAHQRTIRRLRRLIARKQY
                     TEKWFRKYDTWSAGYYELMATKPF"
     gene            269..457
                     /locus_tag="ACHX5T_004019"
     CDS             269..457
                     /locus_tag="ACHX5T_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468976.1"
                     /translation="MNIYFRIVISLAIIACIYGLIVPALISMKDTVAVISGFALACLT
                     PPCIYAIYKGLSFSKDKR"
     gene            454..1281
                     /locus_tag="ACHX5T_004020"
     CDS             454..1281
                     /locus_tag="ACHX5T_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR8468977.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1325..2074
                     /locus_tag="ACHX5T_004021"
     CDS             1325..2074
                     /locus_tag="ACHX5T_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR8468978.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2071..2640
                     /locus_tag="ACHX5T_004022"
     CDS             2071..2640
                     /locus_tag="ACHX5T_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468979.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2637..2759
                     /locus_tag="ACHX5T_004023"
     CDS             2637..2759
                     /locus_tag="ACHX5T_004023"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468980.1"
                     /translation="MKIEYQDAAGGESMSWPDAIVTLGMVFAVAFVVYSICRWG"
     gene            2764..3006
                     /locus_tag="ACHX5T_004024"
     CDS             2764..3006
                     /locus_tag="ACHX5T_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFR8468981.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3010..3156
                     /locus_tag="ACHX5T_004025"
     CDS             3010..3156
                     /locus_tag="ACHX5T_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468982.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            3165..>3325
                     /gene="xisR"
                     /locus_tag="ACHX5T_004026"
     CDS             3165..>3325
                     /gene="xisR"
                     /locus_tag="ACHX5T_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFR8468983.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPKP"
BASE COUNT          928 a          797 c          854 g          746 t
ORIGIN      
        1 acgccatcct gacaagcgtt ttatcttcaa cgggaatgag atatggaaac gtggagaatc
       61 aaccacagag tatacccgcg cacaccagcg aacaatcaga cgactgcgca gactcatcgc
      121 caggaaacag tatacagaaa aatggttcag aaaatatgac acatggagcg ccggatatta
      181 cgaactgatg gcaacaaaac cattctgacg taaacgaaat taaccatgac gcaattaaat
      241 aaggcaagcc gaatacatca ggaggaccat gaacatttat ttcagaatag ttatatcact
      301 ggcaattatc gcatgtattt acggattaat cgttccggcc ctcatatcaa tgaaggatac
      361 ggtagcagtg atttctggct ttgctctggc gtgtctgacc ccgccctgca tttatgccat
      421 ttataagggt ctttcttttt ctaaggataa aagatgaaaa aagttattcc tgctttagcc
      481 cttgttctgc tgaccaccgg acttgtcggt tgcgatcgcg ttgagcctgg taatgttggc
      541 atcaaggtaa acaaactggg cgacgataaa ggcgtcggag aagtggtcgg tgttgggcgc
      601 tactggacgg gatggaacac tgaggtttac atcttcccga ccttcaaaca aatgaagacc
      661 tacgatgagc cattcagctt ccagatgagt gacggtacaa ccatcggcta tcacatcggc
      721 gtggcctaca aagttgatcc atccaaagtt accacagtct ttcagaccta ccgcaaaggc
      781 gtggacgaca ttaccgacac cgacctgcgc cagaagatag ccgacgcact caaccgactg
      841 gccagcaaaa tgaccaccga caaatttatc gacggcggca agtctgagct gctggatgca
      901 gctcttaaag acattcagga agaaatgacg cccatcggta ttcaggtaat gagcctctca
      961 tatgtgggta agccggagta cccgcctact gttatcgaca gcattaatgc caaagtcacg
     1021 gcgaaccaga aaaccctgca atgcgagcag gaagtaaaac agcgcgaagc ggaagccaac
     1081 atgttgcgcg cggaagctgc cggacaggca gatgcgattc gcacaaaagc ccaggccgaa
     1141 gccgatgcta ttcgtttacg cggtgaagct ctgcgccaga acccaaacgt tatggagctg
     1201 gaagccatca acaagtggaa cggtacactg ccgcaataca tgaccagcgg tgccaacaca
     1261 ccatttattc aggttaagta atacatatgc ccggtattac acgccgggct gtctggagat
     1321 aaaaatgaat actgtaacca tcaacaacaa acagtttccg gtaatcgaat atcgcggtca
     1381 gcgtgttgtg acgctggcga tgattgatga agtccaccag cgaccggaag gaaccgcgcg
     1441 tgcagcattc aaccgaaacc gtgagcattt catcagcggt gtggattatg ccgaattagg
     1501 tgcggacgta atacgtacgg acctcccgga aggaacattc tctaaatttg caccatcggg
     1561 gattgtactt tttgaatcag gttacctgat gttgacgaag ccattcaatg acgcccttgc
     1621 ctggcaggtt cagcgcgaac tggttaacag ctacttccgc actcgcacac cgctaacgga
     1681 aatcgagatg atcgctgcga tagccgccga cgctgttcgt caacagaagc gcctgaatca
     1741 tgtggaagaa cagctcgaaa cagtcacaga agcggtggaa accatcaaac gcggaaatat
     1801 gcgcgccgga tatgtcggtt accgccaggt ggtagccaaa agtggaatga gtgacaccaa
     1861 gtgtcggaat ctggtgaacg catatcaaat tcctacagac acccacgaat tcatgactcc
     1921 ggacggtctg ttgtctcgca gggctatcgt cgagcttgag ccatttatgg cggcgttcca
     1981 ccaaatgatg tcagaggctg acccacgcgg cacacgctgg tatcacccga agatggggct
     2041 tttccaggtg atcggatggg aggataaagc atgatcatcc agtcaaaact tattcgcgcc
     2101 gctctggtgt gcgctgctaa aaacgacgtt cgttattacc tgaacggtct tcacatcacg
     2161 ccgaaacata ttgaggcaac caatggttcc gtggcactgc gcatggctca cggcatccgg
     2221 acgaagaaaa acatcattgt ccagttcgaa gggggcgtcc cggccaaagc cgaaacgaca
     2281 gagctgattt ttagtaaaga gccgatcgct gttcatcgcg accagtttca gcgacgactg
     2341 tccattaccg gcattaaact ggtggacggt tgttttccgg atttggatcg catcattccg
     2401 aaaaagtttg accgctgtac tcacccggtg ctccaggcgg gttacctgag ttatccagag
     2461 aagatgtttg gtcgtgagcg taaatttatt cccgtccagt tacgtccctc cggtgacggg
     2521 caagcggtca gaattcagtt tgattccatc atcaactcaa tgtatggcaa tcctgaattt
     2581 gttgtgatgc cttgtcgtga tcatggcgat ttcaacgtgg ctcaggagca tccggaatga
     2641 aaatcgaata ccaggacgcc gcaggaggtg aatcaatgag ctggcctgat gcaatcgtaa
     2701 ctctggggat ggtattcgca gtagcgtttg ttgtgtactc gatttgtcga tggggataac
     2761 cacatgttcg ctttgattca acgcgggcag atatacacag acagagccgg atacccagta
     2821 gaaattactc gcagtactga gcactcagtg ttctttcgga ggatggacgg acgtaccggg
     2881 cgagtacgca tcggtgaatt cagtagcctg ttcgagcata ttgaccacct ggaatatcac
     2941 aagattctgg ctgaaacaga acaagaaaag cacctgaaga aattacgcgc aatgcaaagg
     3001 aagtaaaaaa tgaataaagc gtttgagcta tgggtccacc agcgttacgg caaccactac
     3061 gacctgacgc gagatgttga cggtttctac tgtcgtgaaa ttgtgaaacg aatgtttgag
     3121 gtctggtgcc actgccgtgg gctgagtgtt gtgtgaggta atgcatgggc aatgtgattc
     3181 aactggctcc caatgaatgg gtttgtgaaa gcgttctaat cgcaattacc gggctcaaac
     3241 caggcacaat tcttcggacc cggaaagaat gctggatggt tggaagagag tatattcacg
     3301 tatcaccaga cggtaatcca aagcc
//
LOCUS       DAZSIO010000273         3304 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZSIO010000273 DAZSIO010000000
VERSION     DAZSIO010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.277"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..602)
                     /locus_tag="ACHX5T_004027"
     CDS             complement(<1..602)
                     /locus_tag="ACHX5T_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR8468984.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            1122..2021
                     /locus_tag="ACHX5T_004028"
     CDS             1122..2021
                     /locus_tag="ACHX5T_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8468985.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            2070..3083
                     /gene="yhjD"
                     /locus_tag="ACHX5T_004029"
     CDS             2070..3083
                     /gene="yhjD"
                     /locus_tag="ACHX5T_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR8468986.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
BASE COUNT          834 a          855 c          776 g          839 t
ORIGIN      
        1 caggaggaga tatttaacat cattgccgcc tgggtgcgat ttttcacttc cagacggcga
       61 tacagggatt ccagatgcgc ttttaccgtt ccggtactga tattcagcgc tctgccgatc
      121 tccttatttg attcgcccgc cgctaacatg gttaaaatct cccgctggcg ggcgcttaac
      181 gatttgagat ctttaatgtc cttttccggc gtcgtccgcc agtctccagg cagaaacatc
      241 atccccatcg ccgcactatt taccgccaac gcaaatgtct cgacggttga atcgcgaggc
      301 acaatggcca gcacattaaa atggataact tcctgtaacc accgtttatt gcaatccgtc
      361 gccgtgatta acaccttaac ctcaggaaac tgcaccacgg ttttttgcag caaccagtag
      421 caaaactcac catcctgatc gccatcgagc ataactaagg cttcagggta actttccagc
      481 ttttgccata actcgtctgc ctgactgacc ccctgaatac tcactcctgg aatacgctgc
      541 tgtaaactga tttttattcc atgaataaat attgactgcc tgtcaaacat gactatttgc
      601 ataactgaat ctccacctga atacgttaaa aagacttaag tagtggaagg gtattacccg
      661 cgagaaaaaa taagaattcg ccatttggcg gtggccattc tacagagatg acgcgtagaa
      721 aatagttacc gatataaata gttacagcta aacgcctgaa attacatgtc gagggcacta
      781 tttaaaacaa ttttgaggat ttccttatat tggtggttag tacgcatgca attaaaaatg
      841 aaattccgcg actacaagcc aaaataacaa atggcaatga gacaaaaata agcacaaata
      901 gccaatacgt cctctattca ctttaaaggg aatcactgaa aaatacgctc tgtttaaggg
      961 gattcacctt tctcataaag ctattccgcc cttttcctgc tgagaaatcg ccacattcgt
     1021 catgacaaca ttgtgaaacc cggcattaga tgttagaaaa aacaataaac aatgcgatat
     1081 gcgcgttact gtttcgctgt gaacaacaat cggtcaaaga aatggataaa attcacgcaa
     1141 tgcagttgtt catcaaagtc gcggagctgg aaagtttttc ccgcgcggcg gatttctttg
     1201 ctttgccaaa gggaagtgtt tcgcgccaga tacaggcact ggaacatcaa cttggcaccc
     1261 agcttctcca gcgcactacg cgacgggtca aactcacgcc agaaggcatg acctattatc
     1321 aacgggcaaa agatgtgttg agtaatctca gcgaactgga cggtctgttt caacaggatg
     1381 ccaccagtat cagcggtaaa ttacgcatcg acatcccgtc aggaatcgcg aaaaacctgt
     1441 tactgccgcg tctgtcggaa tttctctatc agcatccggg aattgagctg gaactgagta
     1501 gccataaccg tccggtagat attcttcatg atggttttga ttgcgtgata cgcactggcg
     1561 cgttaccgga agatggcgtt atcgcccgtc ccctcggcaa actaaccatg gtcaactgtg
     1621 ccagtccgca ctatctgacg cgctttggtt atccgcaaag ccccgatgat ctgacttcac
     1681 acgcaatagt gcgttataca ccgcacctgg gtgtacatcc gttaggtttt gaggttgcca
     1741 gcgttaatgg cgtccagtgg tttaagtctg gcggcatgtt gacggtaaac agtagcgaaa
     1801 actatctcgc cgccggtctt gccggtctgg ggattattca gatcccgcgc attgccgtgc
     1861 gcgaagccct gcgtgccggg cggcttattg aagtgttgcc aggctaccgt gccgagccgc
     1921 tctccctttc gctggtttac ccgcagcgtc gggagctttc ccggcgtgta aacctgttta
     1981 tgcagtggct ggctggcgta atgaaagagc acctggactg accgactata ctttttaaga
     2041 atgaccacga cagaaggaca aaagagcgga tgacgcagga aaacgagatc aaacgtccca
     2101 cccaggatct ggagcacgag ccgattaagc agttggataa tagcgagaaa ggcggcaaag
     2161 ttagccaggc gctggaaacc gtcaccacca ctgccgaaaa agtccaacgc caaccggtta
     2221 ttgcgcacct tattcgcgcg acagaacgct ttaacgatcg gctgggtaac cagtttggtg
     2281 cggctatcac ctatttctca tttttgtcga tgatcccgat tttgatggtg tcgtttgccg
     2341 cagggggctt tgtgctggcc tcccatccga tgttgctaca ggatatcttc gacaaaattc
     2401 tgcaaaacat cagcgatccg acactagccg ccacgttgaa aaacaccatc aacaccgccg
     2461 ttcagcagcg tacgactgta gggcttgtcg gcctggcggt ggcactttat tccggcatca
     2521 actggatggg taacctgcgt gaagcgattc gtgcccagtc gcgcgatgtc tgggaacgct
     2581 cgccgcaaga tcaggagaag ttctgggtaa aatatctgcg tgattttatt tcactgattg
     2641 gtttgttgat tgcgctgatt gtgacgcttt cgatcacctc ggttgccggt tcggcgcagc
     2701 aaatgattat tagcgccctg cacctgaaca gcattgagtg gctgaaaccg acgtggcgat
     2761 tgattggcct ggcgatttcc atcttcgcca actatctgct tttcttctgg atcttctggc
     2821 ggttgccgcg ccaccgtccg cgcaaaaaag ccctgattcg cggaacattt ctggctgcta
     2881 ttggttttga agtgattaaa atcgtgatga cctacaccct gccatcgctg atgaaatccc
     2941 cctctggcgc agcatttggt tccgtgctgg ggctgatggc atttttctac ttcttcgccc
     3001 gtttgacgct gttttgcgcg gcgtggattg ccaccgccga atataaagac gacccgcgaa
     3061 tgccagggaa aacgcagcct taaaataggc cggatgcggc gtaaacgcct tatccggcat
     3121 acgattttat tcggcctggc tccccgtagg ccggataaga tgcgccagca tcgcatccgg
     3181 ctataatgcg cacataacct cttgaaactc ttccccagag cctcttcagc catctatttg
     3241 ggagcaaaca atttcactcc aactcataac cccagcatat aaatccagtt ggtaactttt
     3301 attt
//
LOCUS       DAZSIO010000274         3304 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZSIO010000274 DAZSIO010000000
VERSION     DAZSIO010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.278"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..171)
                     /locus_tag="ACHX5T_004030"
     CDS             complement(<1..171)
                     /locus_tag="ACHX5T_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HFR8468987.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMATFAVMTPTY"
     gene            complement(277..1293)
                     /gene="phnD"
                     /locus_tag="ACHX5T_004031"
     CDS             complement(277..1293)
                     /gene="phnD"
                     /locus_tag="ACHX5T_004031"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR8468988.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(1318..2106)
                     /gene="phnC"
                     /locus_tag="ACHX5T_004032"
     CDS             complement(1318..2106)
                     /gene="phnC"
                     /locus_tag="ACHX5T_004032"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR8468989.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            complement(2239..2682)
                     /gene="yjdN"
                     /locus_tag="ACHX5T_004033"
     CDS             complement(2239..2682)
                     /gene="yjdN"
                     /locus_tag="ACHX5T_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFR8468990.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(2840..3175)
                     /locus_tag="ACHX5T_004034"
     CDS             complement(2840..3175)
                     /locus_tag="ACHX5T_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR8468991.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT          689 a          851 c          932 g          832 t
ORIGIN      
        1 gtaagttgga gtcattaccg cgaaggttgc catgttgccg ccgtctttaa tcagcgtaag
       61 cggggccatt tccgcgccct gccacgagac gaccagcacg tcgagaacaa cggcccagct
      121 cagaagcgag aaccagctac gcttgggtgg ggcgatggtg atggtttgca tgtttggctc
      181 cggtctgtag gccggataag gtgcttgcac cgcatccggc atcaacgcct acacattgtc
      241 ggatgcgacg ccggggcgtc ttatccgacc tacgatttac tgcaccgctt tactcaccga
      301 actcatcgcg cttaacgcgt tgttcaggcg gtccaggtca tccagctgcg cctgaatctc
      361 ggtggttttc gccagcttgt cctgctcatt cagcccttta ttgcctttca cgctttgtat
      421 ctctttaaac agtgcgagct ggcgaatcgg caccagttgc aggtcgctgg aagcgcggaa
      481 cggcgcccag cccaggcgtt ccagcaccgc tttctcttcc ggcgtttttc cgtagttcat
      541 aaagaagtcg tagatcttgt ctttggtagt ttcggacaga ttcttgcgcc agacgatcgg
      601 atcgcctagg atcagcggcg atttccagat caccttcagc tctttaagct tctccggcgc
      661 ggaggttttc agcttgtcga ggttttcggt gttgttggtg gcaacatcca cttgcttgtt
      721 ggcgacggcc agcgcgttgg tttcatgccc ggcgttgacg gtgcgcttga agtcgctggc
      781 ggagatattg tttttagcga agacgtagta accggggacg aggaagccag aggtggagtt
      841 aggatcgcca ttgccgaagg tgagatcttt ccgcttcgcc agcagatcgt tcaggttgtt
      901 gatcgggctg tctttgttga cgatcaacac gctccagtaa cccggcgatc catccgccgc
      961 gaccgtctgg gcgaagacct ggccattggc gcgatccacc gcttccatcg ccgacaggtt
     1021 gccgtaccag gcaatatcca ctttattgaa gcgcatcccc tggataatgc ccgcgtagtc
     1081 cggggcaaag aaggcgttga ccttcacgcc cagtttcttc tccatatcct gtaaaaacgg
     1141 cgtccattgc ggtttcaggt tttgctgtga ttccgttgaa ataatgccga aattcagcgc
     1201 cttttcctgc tcttcggcgt gtgccggggt taacagggtg ctgaggctga acatgctggt
     1261 gaaggccagc gaggcaatta tcttagcgtt cattcgtttt cctcaatgat ggggatatca
     1321 ggcagctttc gcgttctctt cgatgcggtt aatgctgcgg tagagatggt caaaacgttc
     1381 gttatcaaac agttggctgc tgccgtcgta gaagacgtgc ccctgacgca gggcgacgat
     1441 gcgttcgcag tagcgcaggg cgtaatccac ctgatgcagc gtgacgacca cggtgatgcc
     1501 gtcgttctgg ttgatgtcgc gcagggtgtc catcacgatg cgcgctgatt ccgggtccag
     1561 cgaggcgatg ggttcatcgg ccagaatcac tttcgcctgc tgcatcagcg cacgggcaat
     1621 cgccacacgt tgctgctggc cgccggagag ggtggaaacg cgctgatggg caaaatgcac
     1681 catgccaacg cgggtcagcg cctgtaacgc gcgctgtttc tgctcgccgg tgaaccagct
     1741 aaaacaggtg cgccagaacg gcgtgctgcc gagcgcgcca atcagcacgt tctccagtac
     1801 gctcaggcgg ttcaccaggt tgaattgttg aaagatgtag ccggtgttgg cgcggctttt
     1861 gcggatatcg cgggccaggc ggccttcgcg ctggacagtg cggcccagta gctcgatatg
     1921 gctaccggcg gatttatcgc cggtaatcaa accgcttaaa tgacgtaaaa gggtggattt
     1981 gccggaaccc gacggcccaa gcagagccac catttcactg tgatgaatgt tcagatcaac
     2041 cgcatgcagc gcttgatgct gattgaaggt tttggcgagc ttctcgacac ggataatcgt
     2101 ttgcatgatg cagcctccct aaaaaagtgg ctccatcgtg gcggattatt gtgacgattt
     2161 ggttaattaa aagtgactaa cagatgaaga gttaacggga attcgatgac agtgcgggag
     2221 ggcgggcctc ccggcgggtt attgcgttgg ttgttgtttg acgacattaa tcatccacgg
     2281 tacgccaaat ttatcggtga ctttgccaaa gccatgcgcc cagaaagttt cctgccaggc
     2341 catttcgatt tttccgttag cggcaaggtt atcaaaccag cgttttcctt cttcgacctg
     2401 ttgcgaatcg agcaccagcg taaagccgga gtagctggct tttcctgacg gtatagcatc
     2461 gctcatcatg atgtcgcttc cggcaatgcg cacgttggca tgagcgatgg cggtatcggg
     2521 aaattgcatt ccggaagggc agttctcggc gctgtcctgc gctgatttcg gcatttcgcc
     2581 gaagctgatt ttatagagca gttccgcgcc caacgtacgt tgataatagg caatcgcgtc
     2641 ggaacagtta ccggcaaaag agaggtaggg acttaacggc atgatggtga cctcagttaa
     2701 gagaagccag ttaagtgtag ttcgaaattt atacagatga gaggctgcct gataagacgc
     2761 gcaagcgtcg catcaggcag tcggcatcgt tgccggatgc ggcgcgagcg ccttatccgg
     2821 cttaccgatc acaatacaat cagttctttt tcacaaactc agatttcagt ttcatcggac
     2881 caaaaccgtc gattttgcaa tcgatgttat ggtcgccttc aaccaggcgg atgtttttca
     2941 ctttggtgcc aattttcagc atcgaagagc tacctttcac cttcagatct ttaatgatgg
     3001 taacgctgtc gccgtcagcc agcagattgc cgttagcatc tttaacgatc agctcgtcgc
     3061 tttcctgtgc aggttctgcg tcgttccatt cgtaggcaca ttccgggcag atgtacatgc
     3121 cgttatcttc gtaagtgtat tcggagttgc attttgggca gtgtggtaat gacatgtgga
     3181 tttcctcaaa agtcagcagg gcaaaagcgc caaaaaacgg cagattgccg aaaaaggccg
     3241 caattataca caaaatccct gagtttgtcg ggggtactgc tgattttaga cgacgatcgt
     3301 tttc
//
LOCUS       DAZSIO010000275         3281 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZSIO010000275 DAZSIO010000000
VERSION     DAZSIO010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.279"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..263
                     /gene="yobA"
                     /locus_tag="ACHX5T_004035"
     CDS             <1..263
                     /gene="yobA"
                     /locus_tag="ACHX5T_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR8468992.1"
                     /translation="AQVTAAPQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNE
                     QDQKQLIVPLADSLKPGTYTVDWHVVSVDGHKTKGHYTFSVK"
     gene            267..1139
                     /gene="copD"
                     /locus_tag="ACHX5T_004036"
                     /pseudo
     CDS             267..1139
                     /gene="copD"
                     /locus_tag="ACHX5T_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            1152..1493
                     /locus_tag="ACHX5T_004037"
     CDS             1152..1493
                     /locus_tag="ACHX5T_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR8468993.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1886..2963)
                     /locus_tag="ACHX5T_004038"
                     /pseudo
     CDS             complement(1886..2963)
                     /locus_tag="ACHX5T_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2929..3210)
                     /locus_tag="ACHX5T_004039"
     CDS             complement(2929..3210)
                     /locus_tag="ACHX5T_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR8468994.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT          784 a          750 c          751 g          996 t
ORIGIN      
        1 acgcgcaagt gacagctgcc ccgcaggcaa tcactttaaa cttctcggaa ggcgttgaaa
       61 caggattcag tggtgcaaaa atcacggggc caaaaaacga aaatattaaa acattaccag
      121 cgaagcggaa tgagcaggac caaaagcaac tgattgttcc actggccgat tcgctgaaac
      181 ccggaaccta caccgttgac tggcatgttg tttcggtgga cgggcataaa acgaaaggac
      241 actacacctt tagcgtgaaa taaatcatgc tggcgtttac ctggatcgcg ctgcgattta
      301 tccatttcac ctcgttgatg ctggtctttg gctttgcaat gtatggtgcc tggctggccc
      361 ccttaatgat tcgtcgcttg ctgacgaagc gttctctgcg tttacaacag cacgctgccg
      421 tctggagttt gatcagtgcc actgcaatgc tcgccgttca gggcgggttg atgggaacgg
      481 gatggacaga tgtattttcg ccaaacattt ggcaggctgt tttgcaaacg cagtttggtg
      541 gcgtctggtt atggcaaatt gttctcgccc tcgtaacgtt gatcgtcgcc cttatgcaac
      601 cgcggaatat gccacgtttg ctgtttatgc tcactaccgc gcaatttatt ctgctggcgg
      661 gagtggggta tgcgacgctg aatgaagggg taacagcgaa aatccatcag actaatcacg
      721 cgattcacct gatttgtgcg gcggcgtggt ttgggggatt actgccggtg ctctggtgta
      781 tgcaactcat caaaggccgt tggcgacact aggctattca ggcgctgatg cgtttttcct
      841 ggtgcgggca ttttgcggtg atcggcgtac tggcaagcgg cgtgcttaat gcattgttga
      901 ttaccggatt tccccccacg cttacgactt actggggcca actcttattg ctcaaagcca
      961 tcctggtgat gattatggtg gtcattgcgc tggctaatcg ttatgttctt gtaccgcgca
     1021 tgaggcagga tgaagatcgt gcggcaccct ggtttgtgtg gatgactaaa ctcgaatggg
     1081 cgataggtgc ggtagtgctg gtgatcatca gcctgcttgc gactctcgaa cctttttgat
     1141 ggattacgaa aatgatgaaa aaaagtattc tggcgtttct gttactcacc agttctgcag
     1201 cggcgctggc ggctccacag gtgataaccg tgagtcgttt tgaagtgggt aaagacaaat
     1261 gggcgtttaa tcgcgaagag gtaatgctga cttgccgacc gggtaatgct ttgtatgtca
     1321 tcaacccaag taccctcgtg cagtatcctt taaacgatat cgcacaaaag gaagttgcca
     1381 gtgggaagac caatgcccaa cccatttcgg tgattcagat tgatgatcct aacaatcccg
     1441 gcgaaaaaat gagtctggca ccgtttatag aacgagctga aaaactctgt taattaccta
     1501 aaatagcctt ttgatttcca ataaaaaaac cgcctcagtt ctttcaccag aacgggcggt
     1561 ttttaacatt taagctgatg agcaccgcgc tttttattga ccattttgca cgcaaactgg
     1621 aaaacctggc gtcgtcatct attcttaaag ggcaaggcaa ctaagcctgc attaatgcca
     1681 acttttagcg cacggctctc tcccaagagc catttccctg gaccgaatac aggaatcgta
     1741 ttcggtcttt ttttaattgt atttaaaatc aattagttgc aaacgtctcc ccgaaattcc
     1801 ccgaaattta ctcgaatttc tgtattccgg tcttttttgg ttatatcaca accaaaatac
     1861 atttaacaat ccatttacgt taaaatcaga gcagtaagta cgttttttct ctctcatcaa
     1921 gatacatttt tgttgtcttc tccgatgtgt ggccaagtag acgctgagca aattcttctc
     1981 cacatatttc tttgtacaat cgtccagcca ggcttctgat ttcgtgaaaa gttggtgggt
     2041 tttcactgaa cagtgatacc tgttaatttt ctggctgcga caaatttctt tgtcaggccg
     2101 tccgggtgaa tgctgccgtc agggctgttt tttctaatcc cggcactgat tagataatcc
     2161 cccctactta ccatgcggca ctgttcaact actgcaccaa gtcgtaaacc agcgacagga
     2221 aggctgagtg acagggggat agcaatcatc attcctgtct taatttgcct gatgtggaga
     2281 cgatcatcat aaatatcact gaaccgcata ttcgttatgt cttcgcgacg ttgtcctgtt
     2341 acaagggcta agtccatagc taatgggaac catgccggaa gttgatctgc tgcctccctg
     2401 atgcagttgt atgtctttag tttcagccgt tctcttgtaa ctactatttt cggtgctctt
     2461 gttggcgtta ctggattttg agatatacgt ccttcaacaa tggcttcgcg gaacatatca
     2521 gataacacag aacgcattga tcctgccatc gtgtttttcc ctccttcaat ctacaaatca
     2581 agaaactcgg cgatatggcg agtggttatt tctgtcagta aaacccctcc taatttttct
     2641 tttattgtct ccagttgatt tacccgagtt ttataagtat ttctggacgc ttttctcctt
     2701 ataagaatcg ttttgtaacg ttcaatccag cctgccatag taaatgagtc gaccccttta
     2761 agcttttcaa ttagggcagc aggagagtag tttttgtata tataatgatt tgcttcaatt
     2821 gcctgcgcta ctgcatctct tcttgaaatt ttacctagtg taaattcttc tttcgtcaga
     2881 gggttgcgcc agtaatatgc tttgtctctc cttcgatatg ttaagtttct aggcaaattg
     2941 ggatcgtatt ttttccgctg catgttttaa cttctccatt aacggactgt ctctcccttg
     3001 tcgcccatta ggctgatggt gtgttatatc ggtatcaacc ttatttgggt tgatatagaa
     3061 agcctccgga accaccctgt aactcctccc gtgtagttca ggtgcaggat aaatgtttcc
     3121 attccttgcc catcgtctca gcgttgatat tgatggtggg ttatccggat atctgagttt
     3181 tccccacgtt ttgagtgtca caagattcat tgccatacct ctcacgatat gaccgccagt
     3241 aaatatacag aatactggcg ggtgtggttg atttttaata a
//
LOCUS       DAZSIO010000276         3251 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZSIO010000276 DAZSIO010000000
VERSION     DAZSIO010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.280"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            61..755
                     /locus_tag="ACHX5T_004040"
     CDS             join(61..307,307..755)
                     /locus_tag="ACHX5T_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR8468995.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(874..1272)
                     /locus_tag="ACHX5T_004041"
     CDS             complement(874..1272)
                     /locus_tag="ACHX5T_004041"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8468996.1"
                     /translation="MNSTINVFGEPSKNLAMLIEEGNLNAPAILNDIKKHVNHLISLH
                     PVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAISIKNHLGQLNLLNSSNIGTLQ
                     INTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(1269..1682)
                     /locus_tag="ACHX5T_004042"
     CDS             complement(1269..1682)
                     /locus_tag="ACHX5T_004042"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HFR8468997.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKN"
     gene            complement(1716..3176)
                     /gene="gadC"
                     /locus_tag="ACHX5T_004043"
     CDS             complement(1716..3176)
                     /gene="gadC"
                     /locus_tag="ACHX5T_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glutamine/gamma-aminobutyrate
                     antiporter GadC"
                     /protein_id="HFR8468998.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
BASE COUNT         1033 a          675 c          641 g          902 t
ORIGIN      
        1 atgggtgatg cctctaatta gttgaatctg atgtataata cgggcttttg aggttatctc
       61 atgaccagcg ttaatattca ttgtccccgt tgtcagtcag ctcaggttta ccgccatggt
      121 cagaacccta aaggccgtga cagacttcgc tgccgtgact gccaccgtgt gtttcagttc
      181 acttatactt atcaagcacg taagccgggt atgaaagagc tgataactga aatggccttt
      241 aatggtgccg gggttcgcga taccgccagg acactgaaaa ttggtagtaa caccgtcatc
      301 cggactttaa aaaactcgcg ccaaagcgaa taacttcttc gcctgttgcc catgctgatg
      361 tggcgtttat ctgcgagctt gatgagcaat ggagctacgt tggcagtaaa gcccggcaac
      421 attggctcgg gtacgcgtac aacaccaaaa caggcggtgt actggcctac acttttggtc
      481 cccgaaccga tcaaacgtgc cgggagctac tggcactgct tacacccttc aacatcggca
      541 tgctgaccag cgatgactgg ggcagctatg gccgggaggt gccgaagaat aagcatctga
      601 ccggcaaaat attcacccaa cgcattgagc gtaataatct gacgctacgc acccgcatta
      661 agcggttggg tcgtaaaaca atctgcttct cacgctcagt tgagatccac gaaaaagtga
      721 tcggagcgtt tatcgaaaaa cacatgttct actaattgga tgcactaccc agtatgtaac
      781 tgggtttgtc cataaaatgc accttactca gttggtgtcc aacttttggg gtgcagttca
      841 gaaggacggg attttacggt gtaccggata aatttaaaac tgcctgatac tgaatgtatg
      901 aggtttcgta atgctcaatt cccctagtac cgttttgtag atctccatac ttccactagt
      961 atttatttgc aaagttccta tatttgagga gtttaataaa tttagctgtc ccaagtgatt
     1021 tttaatagag atagcctgat catgtgcagg attaataaat tttatatccg gtgcaactgc
     1081 ttcaaaaaaa ttctgaacaa ttggatagtg agtacagccg agaataattt catttactgg
     1141 atggagggaa attaaatggt taacatgttt tttgatgtca ttcaagattg caggagcatt
     1201 aagattacct tcctcaatta acattgcaag atttttgctt ggttctccaa atacatttat
     1261 tgttgaattc aattttttat tagctctttg tgacaccccg ttttaatagt aaactcggta
     1321 gcgataatac caacattttt atattgctgg cgaatcacat cttctactgc aggttcaatg
     1381 atggataaga taggaaacgg aaagctctta gcatatccgc tgtattcttc ggattccaaa
     1441 gttgaggata tagtattaca tgcgacagca actagtttga cattccttga ctgtaaaaaa
     1501 gtcagcattt ttttagtcaa actgatgata tcatcggcag aacggttgcc ataaggtgca
     1561 ttcatattat ctccacaata gataatatct tcaccaggaa gcaaattaat tatttccttt
     1621 accgatgtca atccaccgac acctgagtct attacaccaa ttggagattc agcactaaac
     1681 atatttaacc tcatcttttg tagcttatgg aaaatttaat tactagttct tttttcccaa
     1741 gaaaatggtt cgaaatcaga gtttggatct ttccagtcag attttcggaa ggcgtaaatt
     1801 aatattggaa ttaataaaaa tagggcaact aatactaaca aataaagaac atagctggtt
     1861 ggactaccag ttgggatttg ctcgggcgga ataaaactta atacaaaagc cagaaaagac
     1921 cctaagaatc caagtgttgc ccagaaaaat aaagcagggg ctcgatatgg gcgttttgta
     1981 tcaggttgag aatgtcgtag atagatagca ctagcgaaca tgagcatata cataactaaa
     2041 tagagcataa ctgttaactg tgataaaatc tgaaaagctg cttgaactga aggcattatc
     2101 acaaaagtaa cagaaagaac tgagacaatt ataccttgta caaacattag atggtgcccc
     2161 attccatgac gattggtttt ttggaaaaac ttaggtaaat aaccagcttt cgcgatcgct
     2221 aatacacctg tattaggccc tgcaatccaa gtaaccaccc cacccagtac gccaattgct
     2281 agcataaatg ccatcacaga gccaagccat ggcactccag cccattcgaa cagcatatcg
     2341 taggcaacca acaaactttg agttagacta attttatctg taggaataat aaaggcgacc
     2401 cctaaagtac tgagcaggaa tattgcaata gtacctatag aagcgatggt aattgcaatt
     2461 gggtattttt tagttggatt atctaaatcg ttaacatgga ccgcattcat ttccatccct
     2521 gcataagcaa gaaatatgct ggcggcaaga acaatattct ggaagttagc tatattaggg
     2581 aaaagatcac cccaacctaa tgggatcacc gctggtttac cgctgaacca cagataggag
     2641 aaaccaagaa taacaaggat aatagctgga ataattgtcc ctataatacc gcaccactta
     2701 gatacctgcg agaaaatacc aacgccacgt aatgcaacac aaatagctcc ccagtaaaca
     2761 aatagtacga aaaataaaat ataccattta ttactggcaa ttgattctgc cacccccata
     2821 tcaacatcaa taaatgcaac cgagactgca ccaaatgtca gtactgttgg aaagtagggg
     2881 atgacttgta gccaggtcat aagcattcct acaaaacccc agcgagcccc aaatgcttct
     2941 cctatccatc gaaatgcccc ccctttttgt ggatatgttg tagctagctc agccgcaaca
     3001 aaggcaacag gtactaaaaa cataattgca gcaaaggtat agtaaaaaat ggcacctaag
     3061 ccgtattctg cttcagaagg tataaatcgt agacttacta cagcagttat attcatgata
     3121 gcgagtgtcc ccactgtcat tttcttactt ttgggtgcga tattagcatt agacattttt
     3181 atactctcga acatttttaa aatagaaaca tataattacg atgtaaatat aaaaactaat
     3241 attaacttta a
//
LOCUS       DAZSIO010000277         3179 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZSIO010000277 DAZSIO010000000
VERSION     DAZSIO010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.281"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            418..1527
                     /gene="wzz(fepE)"
                     /locus_tag="ACHX5T_004044"
     CDS             418..1527
                     /gene="wzz(fepE)"
                     /locus_tag="ACHX5T_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR8468999.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(1772..1906)
                     /locus_tag="ACHX5T_004045"
     CDS             complement(1772..1906)
                     /locus_tag="ACHX5T_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR8469000.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(2552..2722)
                     /locus_tag="ACHX5T_004046"
     CDS             complement(2552..2722)
                     /locus_tag="ACHX5T_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR8469001.1"
                     /translation="MNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHELAEELQNSI
                     QIRFEAESETGT"
BASE COUNT          801 a          659 c          774 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg ggggggaccc tgagcaggtg gtaattgtaa gacgttgcgg
     2821 gcgacggtat tacaattgca catcctgtcc tgtttcttta ggacgtatca tggcacagca
     2881 tatcaactga taaataatcc ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc
     2941 gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga gtctgttctc
     3001 atgcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt ctttataaat
     3061 agagagttat gacaaattag ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc
     3121 ggcgcggtgt tgtagctgcg ccaacaccgc tttttagggg tggtactgac tattttcat
//
LOCUS       DAZSIO010000278         3169 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZSIO010000278 DAZSIO010000000
VERSION     DAZSIO010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.282"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(7..633)
                     /gene="dsbA"
                     /locus_tag="ACHX5T_004047"
     CDS             complement(7..633)
                     /gene="dsbA"
                     /locus_tag="ACHX5T_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR8469002.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(650..1636)
                     /gene="srkA"
                     /locus_tag="ACHX5T_004048"
     CDS             complement(650..1636)
                     /gene="srkA"
                     /locus_tag="ACHX5T_004048"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR8469003.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1713..1982)
                     /locus_tag="ACHX5T_004049"
     CDS             complement(1713..1982)
                     /locus_tag="ACHX5T_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR8469004.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2052..2636
                     /gene="mobA"
                     /locus_tag="ACHX5T_004050"
     CDS             2052..2636
                     /gene="mobA"
                     /locus_tag="ACHX5T_004050"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR8469005.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2618..3145
                     /gene="mobB"
                     /locus_tag="ACHX5T_004051"
     CDS             2618..3145
                     /gene="mobB"
                     /locus_tag="ACHX5T_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR8469006.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          832 a          770 c          762 g          805 t
ORIGIN      
        1 attgaattat ttttcctcgg acagatattt caccgtatca gcatactgct gaacaaaaac
       61 atccatattg ctggtatcca taccctgcgg attcagctga tatttaccgt taacaaacat
      121 cgccggaaca ccacgcaatt gcacatcagc tgcggctttt tcctgctgag cgaccagaga
      181 tttcaccacg aagctgttcc acgccgcgtc gtactcttca cctttaatac ctgcattgat
      241 aaatacatcg cggatatcag atgctgaacg aatggtctgg gttttctgta cgccttcaaa
      301 cagcggaaca gtcactttgt cttccacgcc cagcgccatc gccacagccc atgcctgagt
      361 cagatctttg cccaggtcac cacccatgaa gttgacgtgg tatttagtca tcttcacgcc
      421 ttccggcagt tttttcttca cattatcaga aatatgcaga acttcttcaa actgatagca
      481 gtgcgggcag aagaaagaga aaaactccag cacttgcggc gcgccagcta ccggtttttc
      541 cagggtagtg tactgtttac catcttcata ctgcgccgcc gatgcgctaa acgctaaaac
      601 taaaccagcc agcgccagcc aaatcttttt catgatctac tctctccgat taatacatag
      661 gtgttaattg caaagggggt tcttgtagaa cttttgcctg ttctataaaa gtcgccgtct
      721 gtcgtagcca gtaatcttcc ccggttaacc acgggaaatt tttcgggaac gcgggatcag
      781 cccaatgccg cattagccag gcaagataat aaaccaaacg catggcgcgt aaaggttcaa
      841 tcagtccgat ttcagcggtg tcgaactcgc taaattcttc ataagcttca ataatagttt
      901 ccagttgcat ccgctgctgg gctttatcgc cattgagcaa catccacaaa tcctgaatag
      961 ctggaccatt acgtgcatca tccagatcaa caaacattgg accatcgcgc cagagaatat
     1021 tcccggcgtg gcagtctcca tgtagccgca gaacggtgaa atcttcccgc cagtgtgctg
     1081 taacggcggc gatcagctca tctgtcgctt tcagaaatgc cgctttcaac ccggaaggta
     1141 tcagtgtagc gtcctcaaac agcttgcgag gctcaatgag atattcattc agaccgatgg
     1201 tcgggcgatg aataaaaagc tgtttgcgcc ccgtctggtg catacgccct aaataacgcc
     1261 caaccgcttc catctgatcg atattatcag cttcgaactg gcgaccaccg acgcttggaa
     1321 aaacagcgaa ataaaatccc tgatgattca ataaagtctg accgttaaag gccacaggtg
     1381 ctgcgaccgg aacttcatca tttaccagct gcaacgcaaa ttgatgttct tcgaggattt
     1441 gatcggctgt ccaacgttca gggcgataaa atttgacgac aaaacgtcga cgctcttcgt
     1501 cctgaaattg atagacacgg ttttcatagc tgttaagcgg ggtaagaccg gaatccaccc
     1561 ggatcccttg cttaaacaga gcgtccatga tggtatccgg gtgtagtgtc tggaaagtaa
     1621 aagcgctgtt gttcatccca tcatccggaa aacattacga atgatgaagg atatcatttc
     1681 acgatgattt tggtggcgcc gcttacaagc ttttactctt taattacgcc gcgagcgcgt
     1741 aacagcgccg ttttgaagtc ttcttcataa tctttctgca aacccgggat caccgcatct
     1801 ttcgcggaat cacgcatttt caggtgatag atcagaatgt catccgtcaa atccgccagt
     1861 tcgccgtcaa aacctgactc tttcgccagt ttctgcaaaa attgcaacaa gttaaagtct
     1921 ggctcttttt gccaggctgg ctggaggagt tcaataactt cattcagacg tttacatttc
     1981 atagtaatgc tccttactct tgagacagac acgttagcag ggtcaatccc acaataaaag
     2041 aggcgatatc ggtgaatcta atgacgacga taacaggcgt tgtgctggca ggcggtaaag
     2101 ccagacgaat gggcggcgta gataaaggat tgctggactt aaatggcaaa ccgctgtggc
     2161 aacatgtcgc tgacgcgctt aagacgcagc tctctcacgt cgtggttaat gctaatcgtc
     2221 atcaggaaat ctatcaggca ggcggtctga aagtgattga agattcactg gcggattacc
     2281 caggccctct ggcaggaatg ctttcagtaa tgcagcagga agctggtgag tggtttttgt
     2341 tttgtccgtg cgatacgcct tacattcccc atgatttagc cgcccggctt aatcatcagc
     2401 gcaaagatgc gcctgtcgtg tgggtccatg acggtgaacg cgatcacccg actattgctc
     2461 tggtaaaccg cgctattgag cctttattac tggaatatct gcaagcagga gaacgccggg
     2521 taatggcatt tatgcgtctg gctggcggtc atgcggttga tttcagcgat cataaagatg
     2581 catttgttaa cgtgaatacg ccagaggagc ttgcccgatg gcaggaaaaa cgatgatacc
     2641 gttactcgcc tttgccgcgt ggagtggtac cggaaaaact acgcttttga aaaaactgat
     2701 cccggcatta tgcgccagag ggatccgtcc ggggctgatt aagcatacgc accatgatat
     2761 ggatgttgat aagccaggca aagatagcta tgagctgcgc aaggctggcg cggtacaaac
     2821 catcgttgcc agccagcaac gatgggcctt gatgacggaa acaccagacg aagaagagct
     2881 ggatctacat tttctcgcaa gtcgaatgga tacctcaaag ctggatttga ttctggtcga
     2941 agggtttaag catgaagaga tcgcaaagat tgtgctgttt cgcgatggag ccggacatcg
     3001 acctgaagaa ttagtgatag acaggcatgt tattgctgta gccagtgatg tgccgcttaa
     3061 tcttgatgtc gcattactgg atattaatga tgttgagggg ctggctgatt ttgtggtgga
     3121 gtggatgcaa aagcagaacg gataagcaag agtaggctgg atcagacgc
//
LOCUS       DAZSIO010000279         3142 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZSIO010000279 DAZSIO010000000
VERSION     DAZSIO010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.283"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(108..479)
                     /locus_tag="ACHX5T_004052"
     CDS             complement(108..479)
                     /locus_tag="ACHX5T_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469007.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(540..1037)
                     /locus_tag="ACHX5T_004053"
     CDS             complement(540..1037)
                     /locus_tag="ACHX5T_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469008.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(1066..1901)
                     /gene="aadA1"
                     /locus_tag="ACHX5T_004054"
                     /pseudo
     CDS             complement(1066..1901)
                     /gene="aadA1"
                     /locus_tag="ACHX5T_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
     gene            complement(1959..2483)
                     /gene="sat2"
                     /locus_tag="ACHX5T_004055"
     CDS             complement(1959..2483)
                     /gene="sat2"
                     /locus_tag="ACHX5T_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR8469009.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(2578..3051)
                     /gene="dfrA1"
                     /locus_tag="ACHX5T_004056"
     CDS             complement(2578..3051)
                     /gene="dfrA1"
                     /locus_tag="ACHX5T_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR8469010.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
BASE COUNT          753 a          793 c          737 g          859 t
ORIGIN      
        1 cataccttgg cctaggtaaa gaagatcaga ctcgtagcca catcggtggc aactgagctt
       61 gtacgcaatg cccatctggt tgtctcacct gaatcgtaac gtcagagcta ttcatccatc
      121 cattcggcat caatgtatgc cactaagccg accagctgac cgtttggact gaagtatgtt
      181 gcctgcaccc cacttgacat ctcatcaata ccaccgcact gcgctaggcg ccggtcccac
      241 ttgcccacag aagcgtcagg atcggctgag ttccccaaga cccttgtgag ccatgcctcc
      301 tttaactcaa cctctcgaat gattgcgtcg gccagctcct ccgcgttatc tagttcgaca
      361 tagtctggaa ttcggacgta cgttctgaac acagctggat acttacttgg gcgattgtca
      421 tacatgacat caacaatgta cccgtttgtg taaccgtctc ttggaggttc gtatgacact
      481 agtggttccc ctcagcttgc gactagatgt tgaggcctaa cattttatta gagagcaggc
      541 tagttgctta gatacatgat cttcaggccg ttatctgtca gggcaagcga aaattggcca
      601 tttatgacga ccaatgcccc gcagaagctc ccatctttgc cgccatagac gccgcgcccc
      661 ccttttgggg tgtagaacat ccttttgcca gatgtggaaa agaagttcgt tgtcccattg
      721 ttggcaatga cgtagtagcc ggcgaaagtg cgagacccat ttgcgctata tataagccta
      781 cgatttccgt tgcgactatt gtcgtaattg gatgaactat tatcgtagtt gctctcagag
      841 ttgtcgtaat ttgatggact attgtcgtaa ttgcttatgg agttgtcgta gttgcttgga
      901 gaaatgtcgt agttggatgg ggagtagtca tagggaagac gagcttcatc cactaaaaca
      961 attggcaggt cagcaagtgc ctgccccgat gccatcgcaa gtacgaggct tagaaccacc
     1021 ttcaacagat cgcgcatagt cttccccagc tctctaacgc ttgagttaag ccgcgccgcg
     1081 aagcggcgtc ggcttgaacg aattgttaga cattatttgc cgactacctt ggtgatctcg
     1141 cctttcacgt agtgaacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct
     1201 tgtccaagat aagcctgcct agcttcaagt atgacgggct gatactgggc cggcaggcgc
     1261 tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac
     1321 caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag
     1381 ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca
     1441 aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc
     1501 aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg
     1561 cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg
     1621 tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc
     1681 gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc
     1741 accgtaacca gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg
     1801 tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct
     1861 gatagttgag tcgatacttc ggcgatcacc gcttccctca tgatgtttaa ctcctgaatt
     1921 aagccgcgcc gcgaagcggt gtcggcttga atgaattgtt aggcgtcatc ctgtgctccc
     1981 gagaaccagt accagtacat cgctgtttcg ttcgagactt gaggtctagt tttatacgtg
     2041 aacaggtcaa tgccgccgag agtaaagcca cattttgcgt acaaattgca ggcaggtaca
     2101 ttgttcgttt gtgtctctaa tcgtatgcca aggagctgtc tgcttagtgc ccactttttc
     2161 gcaaattcga tgagactgtg cgcgactcct ttgcctcggt gcgtgtgcga cacaacaatg
     2221 tgttcgatag aggctagatc gttccatgtt gagttgagtt caatcttccc gacaagctct
     2281 tggtcgatga atgcgccata gcaagcagag tcttcatcag agtcatcatc cgagatgtaa
     2341 tccttccggt aggggctcac acttctggta gatagttcaa agccttggtc ggataggtgc
     2401 acatcgaaca cttcacgaac aatgaaatgg ttctcagcat ccaatgtttc cgccacctgc
     2461 tcagggatca ccgaaatctt catatgacgc ctaacgcctg gcacagcgga tcgcaaacct
     2521 ggcgcggctt ttggtacaaa aggcgtgaca ggtttgcgaa tccgttgctg ccacttgtta
     2581 acccttttgc cagatttggt aactataatt tatgttagag gcgaagtctt gggtaaaaac
     2641 tggcctaaaa ttgctgggga tttcaggaaa gtaaacatca ccttccggct cgatgtctat
     2701 tgtagatata tgtagtgtat ctacttgatc gatcaggctt ttgtatatct ccccaccacc
     2761 tgaaacaatg acatgatccg ttattttctt taggttggtt aaagcatctt taattgatgg
     2821 aaagatcaat acgttctcat tgtcagatgt aaaacttgaa cgtgttacga ccgcatactt
     2881 tcggttgggt aatgctccca ttgattcaaa agtcttgcgt ccaaccaaca gccattggtt
     2941 ataggtaata gctttaaaca ggagctgttc acctttggca ctccatggaa tatcagggcc
     3001 attcccgata actccattct tcgatatagc taccattagt gatagtttca caattcttcc
     3061 tcagaggtta acattttatt agtaagcatg ctcgttttgt cacccgctga tgcttaccgt
     3121 taattaatat aatcacttgt at
//
LOCUS       DAZSIO010000280         3069 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZSIO010000280 DAZSIO010000000
VERSION     DAZSIO010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.284"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..498
                     /locus_tag="ACHX5T_004057"
     CDS             <1..498
                     /locus_tag="ACHX5T_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8469011.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            578..1273
                     /locus_tag="ACHX5T_004058"
     CDS             578..1273
                     /locus_tag="ACHX5T_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR8469012.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1302..>3069
                     /locus_tag="ACHX5T_004059"
     CDS             1302..>3069
                     /locus_tag="ACHX5T_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR8469013.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          888 a          718 c          684 g          779 t
ORIGIN      
        1 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
       61 gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt
      121 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      181 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc
      241 cctaatctgg caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt
      301 attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg
      361 atttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac
      421 gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta
      481 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      541 cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt
      601 gatattaacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      661 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      721 caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag
      781 ccgtgcccca tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc
      841 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      901 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      961 gctggtattt aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
     1021 aaacgatgcg tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa
     1081 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
     1141 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
     1201 aaagaacgtg aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg
     1261 tccactgcaa taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc
     1321 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg
     1381 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1441 ccattatgct ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1501 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1561 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1621 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1681 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat
     1741 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1801 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1861 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1921 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
     1981 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     2041 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     2101 gctacgcctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     2161 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     2221 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     2281 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac
     2341 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2401 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2461 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2521 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2581 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2641 ttcatggcta tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata
     2701 tcgctgttag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2761 cccgcaattg gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2821 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2881 acgagaaaaa cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     2941 gcctgaatta tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga
     3001 atctcagtat tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat
     3061 cacttaacc
//
LOCUS       DAZSIO010000281         3015 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZSIO010000281 DAZSIO010000000
VERSION     DAZSIO010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.286"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(67..612)
                     /gene="syd"
                     /locus_tag="ACHX5T_004060"
     CDS             complement(67..612)
                     /gene="syd"
                     /locus_tag="ACHX5T_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR8469014.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            680..1528
                     /gene="queF"
                     /locus_tag="ACHX5T_004061"
     CDS             680..1528
                     /gene="queF"
                     /locus_tag="ACHX5T_004061"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR8469015.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            1640..3004
                     /gene="ppnN"
                     /locus_tag="ACHX5T_004062"
     CDS             1640..3004
                     /gene="ppnN"
                     /locus_tag="ACHX5T_004062"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR8469016.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT          739 a          748 c          739 g          789 t
ORIGIN      
        1 cttatttcct ggcgatctcc tacagagtct gtaagagatc tctcacaacc agatggggta
       61 tttggattac agaagaggct taagttgatt aaggaattcc gcaagatttg aggctaaatt
      121 cgtccgttta cgtgttccca gcgtctcttt gcacacttca ccggagagat tacacaccga
      181 gatgacctct aactcttctt ccagcgtcgc gataaatagc gtgggcggca gttttagcct
      241 tttctgagtc accagatgac caatcagatt ttcctgcacc cgacggaagt cgtcttcact
      301 ccaggtttgc agcaatgtca gtttgatatc gccaaactgc gcgtgcatat ccccggcaaa
      361 ctgagtcgta taaaaggtat gaattgtggg ttgtatcaca atatcaaaag cgcgttcaac
      421 cgcgtttaca ttttgttccc cggtaaacgg ctggggttgc cagtatacgg catcttcggt
      481 agttgaaata atgcacggtg aaggaacacc gtacaattcc tcacttaacg gccagctttt
      541 atgctcttca tgccacgcat cgcagtagcg tgcagtaaaa tctttcaggg cttgtgcggt
      601 caaatcgtcc actaatttct ctcttcactt aaaccagata cacttgtcgt ttagtttatc
      661 tggtttatga cggtgaaaca tgtcttctta tgcaaaccat caggcacttg cgggcctgac
      721 tcttggaaaa tcaaccgatt accgggatac ctatgacgcc agcctactgc aaggcgttcc
      781 acgcagcctg aatcgcgacc cgctgggtct gaaagcggat aacctgcctt ttcacggtac
      841 ggatatctgg acgctgtatg aactttcctg gctgaatgcg aaaggtttgc cgcaggtcgc
      901 tgtcggtcat gttgaacttg attacaccag cgtaaatctg attgagtcga agagttttaa
      961 gctctatctc aacagtttta accagacgcg ttttaataac tgggatgagg tgcgccagac
     1021 gctggagcgc gacttaagca cttgcgctca gggtaagatt agcgtggcgt tatatcgtct
     1081 tgatgaactg gaaggccagc cgataggtca tttcaatggc acttgcattg atgaccagga
     1141 tatcactatc gataactatg aattcactac tgactatctg gagaatgcca ccagcggtga
     1201 aaaagtagtg gaagagacgc tcgtcagcca cctgctgaaa tcaaactgcc tgatcaccca
     1261 tcaaccagat tggggttcga tccaaattca gtatcgtgga cgccaaattg acagagaaaa
     1321 actgctgcgt tacctggtct cattccgtca tcacaacgag ttccacgaac agtgcgtgga
     1381 acgcatcttt aatgacctgt tacgcttctg ccagccagag aaattgagcg tttacgcacg
     1441 ttatacccgt cgtggcggtc tggacattaa cccgtggcgc agtaatagcg attttgtccc
     1501 atcgaccaca agactggttc ggcaataaat tttttctcaa ttttgcgtgc tggattcacg
     1561 cagaaggttg tgaaaggtca tcaggcaggg ctattgtaat caaagggaat gacgatattc
     1621 gtcccataag gagtttttct tgattacaca tattagcccg cttggctcca tggatatgtt
     1681 gtcgcagctg gaagtggata tgcttaaacg caccgccagc agcgacctct atcaactgtt
     1741 tcgcaactgt tcacttgccg tactgaactc cggtagtttg accgataaca gcaaagaatt
     1801 gctgtctcgt tttgaaaatt tcgatattaa cgtcctgcgc cgtgaacgcg gcgtaaagct
     1861 ggaactgatt aatcccccgg aagaggcttt tgtcgatggg cgaattattc gcgctttgca
     1921 ggccaacttg ttcgcggttc tgcgagacat tctcttcgtt tacgggcaaa tccatcatac
     1981 cgttcgtttt cccaacctga atctcgacaa ctccgtccac atcactaacc tggtcttttc
     2041 catcttgcgt aacgctcgcg cgctgcatgt gggtgaagcg ccaaatatgg tggtctgctg
     2101 gggcggtcac tcaattaacg aaaacgagta tttgtatgcc cgtcgcgtcg ggaaccagct
     2161 gggcctgcgt gagctgaata tctgcaccgg ctgtggtcca ggagcgatgg aagcgccgat
     2221 gaaaggtgct gcggtcggac acgcgcagca gcgttacaaa gacagtcgtt ttattggtat
     2281 gacagagccg tcgattatcg ccgctgaacc gcctaaccca ctggtcaacg aattgatcat
     2341 catgccggat atcgaaaaac gtctggaagc gtttgtccgt atcgctcacg gcatcattat
     2401 cttccctggc ggtgtgggta cggcagaaga gttgctgtat ttgctgggaa ttttaatgaa
     2461 cccggccaac aaagatcagg ttttaccatt gatcctcacc ggcccgaaag agagcgccga
     2521 ctacttccgc gtactggacg agtttgtcgt acatacgctg ggcgaaaacg cgcgccgcca
     2581 ttaccgcatc atcattgatg acgccgctga agtcgctcgt cagatgaaaa aatcgatgcc
     2641 gctggtgaaa gaaaatcgcc gtgatacagg cgatgcctac agctttaact ggtcaatgcg
     2701 cattgcgcca gatttgcaaa tgccgtttga gccgtctcac gagaatatgg ctaatctgaa
     2761 gctttacccg gatcaacctg ttgaagtgct ggctgccgac ctgcgccgtg cgttctccgg
     2821 tattgtggcg ggtaacgtaa aagaagtcgg tattcgcgcc attgaagagt ttggtcctta
     2881 caaaatcaac ggcgataaag agattatgcg tcgtatggac gacctgctac agggttttgt
     2941 tgcccagcat cgtatgaagt tgccaggctc agcctacatc ccttgctacg aaatctgcac
     3001 gtaatctccc ctccc
//
LOCUS       DAZSIO010000282         2991 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZSIO010000282 DAZSIO010000000
VERSION     DAZSIO010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.287"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            9..602
                     /gene="tetC"
                     /locus_tag="ACHX5T_004063"
     CDS             9..602
                     /gene="tetC"
                     /locus_tag="ACHX5T_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HFR8469017.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            complement(715..1920)
                     /gene="tet(B)"
                     /locus_tag="ACHX5T_004064"
     CDS             complement(715..1920)
                     /gene="tet(B)"
                     /locus_tag="ACHX5T_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFR8469018.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            2002..2625
                     /gene="tetR(B)"
                     /locus_tag="ACHX5T_004065"
     CDS             2002..2625
                     /gene="tetR(B)"
                     /locus_tag="ACHX5T_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFR8469019.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            complement(2603..>2991)
                     /locus_tag="ACHX5T_004066"
     CDS             complement(2603..>2991)
                     /locus_tag="ACHX5T_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8469020.1"
                     /translation="HGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITEN
                     GSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGW
                     LTRHLGYREVTITEKGYAAFKTHFHI"
BASE COUNT         1023 a          616 c          566 g          786 t
ORIGIN      
        1 aatctatcat ggaaaataaa aatcatcaac aagaaaattt taagagtacc tatcaatcac
       61 tggttaactc agcacgaata ttgtttgttg aaaaaggcta tcaagctgtt tcaatagatg
      121 agatctcggg aaaagcgttg gtgaccaaag gtgcctttta tcatcacttt aaaaataaaa
      181 aacaattact cagtgcctgt tataagcagc aattaattat gattgatgcc tacatcacaa
      241 caaaaactga tttaacaaat ggttggtctg ccttagaaag tatatttgaa cattatcttg
      301 attatattat tgataataat aaaaacctta tccctatcca agaagtgatg cctatcattg
      361 gttggaatga acttgaaaaa attagccttg aatacattac tggtaaggta aacgccattg
      421 tcagcaaatt gatccaagag aaccaactta aagcttatga tgatgatgtg cttaaaaact
      481 tactcaatgg ctggtttatg catatcgcaa tacatgcgaa aaacctaaaa gagcttgccg
      541 ataaaaaagg ccaatttatt gctatttacc gcggcttttt attgagcttg aaagataaat
      601 aaaatagata ggttttattt gaagctaaat cttctttatc gtaaaaaatg ccctcttggg
      661 ttatcaagag ggtcattata tttcgcggaa taacatcatt tggtgacgaa ataactaagc
      721 acttgtctcc tgtttactcc cctgagcttg aggggttaac atgaaggtca tcgatagcag
      781 gataataata cagtaaaacg ctaaaccaat aatccaaatc cagccatccc aaattggtag
      841 tgaatgatta taaataacag caaacagtaa tgggccaata acaccggttg cattggtaag
      901 gctcaccaat aatccctgta aagcaccttg ctgatgactc tttgtttgga tagacatcac
      961 tccctgtaat gcaggtaaag cgatcccacc accagccaat aaaattaaaa cagggaaaac
     1021 taaccaacct tcagatataa acgctaaaaa ggcaaatgca ctactatctg caataaatcc
     1081 gagcagtact gccgtttttt cgccccattt agtggctatt cttcctgcca caaaggcttg
     1141 gaatactgag tgtaaaagac caagacccgc taatgaaaag ccaaccatca tgctattcca
     1201 tccaaaacga ttttcggtaa atagcaccca caccgttgcg ggaatttggc ctatcaattg
     1261 cgctgaaaaa taaataatca acaaaatggg catcgtttta aataaagtga tgtataccga
     1321 attcgattgc gtctcaaccc ctacttcggt atctgtatta tcacgtgtat ttttggtttc
     1381 acggaaccaa aacataacca caaggaaagc gacaatattt agcaacgcag cgataaaaaa
     1441 gggactatgc ggtgaaatct ctcctgcaaa accaccaata ataggccccg ctattaaacc
     1501 aagcccaaaa cttgccccta accaaccgaa ccacttcacg cgttgagaag ctgaggtggt
     1561 atcggcaatg accgatgccg cgacagcccc agtagctcct gtgatccctg aaagcaaacg
     1621 gcctaaatac agcatccaaa gcgcacttga aaaagccagc aataagtaat ccagcgatgc
     1681 gcctattaat gacaacaaca gcactgggcg ccgaccaaat cggtcagaca tttttccaag
     1741 ccaaggagca aagataacct gcattaacgc ataaagtgca agcaatacgc caaagtggtt
     1801 agcgatatct tccgaagcaa taaattcacg taataacgtt ggcaagactg gcatgataag
     1861 gccaatcccc atggcatcga gtaacgtaat taccaatgcg atctttgtcg aactattcat
     1921 ttcacttttc tctatcactg atagggagtg gtaaaataac tctatcaatg atagagtgtc
     1981 aacaaaaatt aggaattaat gatgtctaga ttagataaaa gtaaagtgat taacagcgca
     2041 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag
     2101 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac
     2161 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa
     2221 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat
     2281 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc
     2341 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca
     2401 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc
     2461 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct
     2521 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc
     2581 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt cttaaaagca gcataacctt
     2641 tttccgtgat ggtaacttca cggtaaccaa gatgtcgagt taaccaccct ttagattcat
     2701 aaagcgaaaa taatgcggct ccaacgtacc cacctaaatg gaaacggcgt tcactccaat
     2761 ctaaacacgc acaacagatt ttacgtgaat gtttggaagg aacgtcaatt cccatttcat
     2821 gaaaatattg aataccactt aatgtgatca ttgaaccatt ttcagtgatc cattgctgtt
     2881 gacaaaggga atcatagatc ttaacggcaa cttcgccagc taaatgatca tagcaagtac
     2941 gtgcttttcg taaatgcact ggcgtggaaa ctttggcatg tacgccatgg t
//
LOCUS       DAZSIO010000283         2988 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZSIO010000283 DAZSIO010000000
VERSION     DAZSIO010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.288"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..2850
                     /locus_tag="ACHX5T_004067"
     rRNA            <1..2850
                     /locus_tag="ACHX5T_004067"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          789 a          659 c          930 g          610 t
ORIGIN      
        1 gacgtgctaa tctgcgataa gcgtcggtaa ggtgatatga accgttataa ccggcgattt
       61 ccgaatgggg aaacccagtg tgattcgtca cactatcatt aactgaatcc ataggttaat
      121 gaggcgaacc gggggaactg aaacatctaa gtaccccgag gaaaagaaat caaccgagat
      181 tcccccagta gcggcgagcg aacggggagc agcccagagc ctgaatcagt gtgtgtgtta
      241 gtggaagcgt ctggaaaggc gtgcgataca gggtgacagc cccgtacaca aaaatgcaca
      301 tactgtgagc tcgatgagta gggcgggaca cgtggtatcc tgtctgaata tggggggacc
      361 atcctccaag gctaaatact cctgactgac cgatagtgaa ccagtaccgt gagggaaagg
      421 cgaaaagaac cccggcgagg ggagtgaaaa agaacctgaa accgtgtacg tacaagcagt
      481 gggagcacgc ttaggcgtgt gactgcgtac cttttgtata atgggtcagc gacttatatt
      541 ctgtagcaag gttaaccgaa taggggagcc gaagggaaac cgagtcttaa ctgggcgtta
      601 agttgcaggg tatagacccg aaacccggtg atctagccat gggcaggttg aaggttgggt
      661 aacactaact ggaggaccga accgactaat gttgaaaaat tagcggatga cttgtggctg
      721 ggggtgaaag gccaatcaaa ccgggagata gctggttctc cccgaaagct atttaggtag
      781 cgcctcgtga attcatctcc gggggtagag cactgtttcg gcaagggggt catcccgact
      841 taccaacccg atgcaaactg cgaataccgg agaatgttat cacgggagac acacggcggg
      901 tgctaacgtc cgtcgtgaag agggaaacaa cccagaccgc cagctaaggt cccaaagtca
      961 tggttaagtg ggaaacgatg tgggaaggcc cagacagcca ggatgttggc ttagaagcag
     1021 ccatcattta aagaaagcgt aatagctcac tggtcgagtc ggcctgcgcg gaagatgtaa
     1081 cggggctaaa ccatgcaccg aagctgcggc agcgacgctt atgcgttgtt gggtagggga
     1141 gcgttctgta agcctgtgaa ggtgtgctgt gaggtatgct ggaggtatca gaagtgcgaa
     1201 tgctgacata agtaacgata aagcgggtga aaagcccgct cgccggaaga ccaagggttc
     1261 ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa ggcgaggccg aaaggcgtag
     1321 tcgatgggaa acaggttaat attcctgtac ttggtgttac tgcgaagggg ggacggagaa
     1381 ggctatgttg gccgggcgac ggttgtcccg gtttaagcgt gtaggctggt tttccaggca
     1441 aatccggaaa atcaaggccg aggcgtgatg acgaggcact acggtgctga agcaacaaat
     1501 gccctgcttc caggaaaagc ctctaagcat caggtaacat caaatcgtac cccaaaccga
     1561 cacaggtggt caggtagaga ataccaaggc gcttgagaga actcgggtga aggaactagg
     1621 caaaatggtg ccgtaacttc gggagaaggc acgctgatac gtaggtgaag cgacttgctc
     1681 gtggagctga aatcagtcga agataccagc tggctgcaac tgtttattaa aaacacagca
     1741 ctgtgcaaac acgaaagtgg acgtatacgg tgtgacgcct gcccggtgcc ggaaggttaa
     1801 ttgatggggt tagcgcaagc gaagctcttg atcgaagccc cggtaaacgg cggccgtaac
     1861 tataacggtc ctaaggtagc gaaattcctt gtcgggtaag ttccgacctg cacgaatggc
     1921 gtaatgatgg ccaggctgtc tccacccgag actcagtgaa attgaactcg ctgtgaagat
     1981 gcagtgtacc cgcggcaaga cggaaagacc ccgtgaacct ttactatagc ttgacactga
     2041 acattgagcc ttgatgtgta ggataggtgg gaggctttga agtgtggacg ccagtctgca
     2101 tggagccgac cttgaaatac caccctttaa tgtttgatgt tctaacgtgg acccgtgatc
     2161 cgggttgcgg acagtgtctg gtgggtagtt tgactggggc ggtctcctcc taaagagtaa
     2221 cggaggagca cgaaggttgg ctaatcctgg tcggacatca ggaggttagt gcaatggcat
     2281 aagccagctt gactgcgagc gtgacggcgc gagcaggtgc gaaagcaggt catagtgatc
     2341 cggtggttct gaatggaagg gccatcgctc aacggataaa aggtactccg gggataacag
     2401 gctgataccg cccaagagtt catatcgacg gcggtgtttg gcacctcgat gtcggctcat
     2461 cacatcctgg ggctgaagta ggtcccaagg gtatggctgt tcgccattta aagtggtacg
     2521 cgagctgggt ttagaacgtc gtgagacagt tcggtcccta tctgccgtgg gcgctggaga
     2581 actgaggggg gctgctccta gtacgagagg accggagtgg acgcatcact ggtgttcggg
     2641 ttgtcatgcc aatggcactg cccggtagct aaatgcggaa gagataagtg ctgaaagcat
     2701 ctaagcacga aacttgcccc gagatgagtt ctccctgacc ctttaagggt cctgaaggaa
     2761 cgttgaagac gacgacgttg ataggccggg tgtgtaagcg cagcgatgcg ttgagctaac
     2821 cggtactaat gaaccgtgag gcttaacctt acaacgccga agatgttttg gcggattgag
     2881 agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga
     2941 attagcctgg cggccgtagc gcggtggtcc cacctgaccc catgccga
//
LOCUS       DAZSIO010000284         2951 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZSIO010000284 DAZSIO010000000
VERSION     DAZSIO010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.289"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="ACHX5T_004068"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="ACHX5T_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR8469021.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="ACHX5T_004069"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="ACHX5T_004069"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR8469022.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="ACHX5T_004070"
     CDS             2603..>2951
                     /locus_tag="ACHX5T_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR8469023.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          701 c          779 g          813 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttctctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DAZSIO010000285         2933 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZSIO010000285 DAZSIO010000000
VERSION     DAZSIO010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.290"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="ACHX5T_004071"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="ACHX5T_004071"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR8469024.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /gene="ssuA"
                     /locus_tag="ACHX5T_004072"
     CDS             643..1602
                     /gene="ssuA"
                     /locus_tag="ACHX5T_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR8469025.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..2744
                     /gene="ssuD"
                     /locus_tag="ACHX5T_004073"
     CDS             1599..2744
                     /gene="ssuD"
                     /locus_tag="ACHX5T_004073"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFR8469026.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2756..>2933
                     /locus_tag="ACHX5T_004074"
     CDS             2756..>2933
                     /locus_tag="ACHX5T_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469027.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELW"
BASE COUNT          659 a          801 c          806 g          667 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacgcctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg ggggaaaccg
     2041 tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc
     2101 aacagcctta tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg
     2161 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc ggaacttgtt aaagagaaaa
     2221 tcgaacaagt gcgggcgaaa gctgccgcgt atgggcgcaa aattcgtttc ggtattcgtc
     2281 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2341 cgcatcttga tgatgagact atcgccaaag cacaagccgc attcgcccgg acggattccg
     2401 taggacaaca gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc
     2461 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2521 atggtcctac ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2581 tgctttcggg ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac
     2641 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2701 cggtggcaaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc
     2761 aacgccagtg aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt
     2821 ggcggtgtgg caactggcct cctcggttgg ctggctgtcg acgcgtattt tgccttcacc
     2881 ggaaggagtg gtgacggcgt tctggacgct ctccgccagc ggcgaactgt ggc
//
LOCUS       DAZSIO010000286         2894 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZSIO010000286 DAZSIO010000000
VERSION     DAZSIO010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.291"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..308)
                     /locus_tag="ACHX5T_004075"
     CDS             complement(<1..308)
                     /locus_tag="ACHX5T_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR8469028.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     "
     gene            complement(355..1074)
                     /gene="btsR"
                     /locus_tag="ACHX5T_004076"
                     /gene_synonym="yehT"
     CDS             complement(355..1074)
                     /gene="btsR"
                     /locus_tag="ACHX5T_004076"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR8469029.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="ACHX5T_004077"
     CDS             complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="ACHX5T_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR8469030.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          748 a          774 c          657 g          715 t
ORIGIN      
        1 tcatcggttt tcagcgaaaa cgcgatgcgt aattttttca gcacgatgtt gttattaatg
       61 cgacgttccg gctccagtgc cggagcagac tcatccttgc cgcgtttttc ataaatcagg
      121 ccattgagga atgacgacaa aacaatgtcc ggacaacgct gaaaaccctc ttcgtcttct
      181 ttacgtagcc agacggcgat ctgttccgcg gtggcttcga cattatccag cgccagaata
      241 cgcaccaggt cattattatt ggctttcaaa atgtagcgca cgctgcgcag aatatcgtta
      301 cttatcatga ggccttcagg tgttgatgag gcaaaaagcc attttagcag tcttttacag
      361 gccaatcgcc tcttttaagc ttttcagata acggcggctg accggcacgg ttaagccatt
      421 acgcaaaatc aactcggcct ggccgttatc ttccagacga atctcctgta aatgcgcgag
      481 gttaaccaga tactgacgat ggcagcgcag tagtggtgta cgactttcca aggtacgtaa
      541 tgtcaattcg gtaaagccct ctttcccttc gtggctggta acgtagacac cgctcatccg
      601 actgctgaca aatgccacat ctttcatttg cagcaaataa atccgactat gccccgtaca
      661 agggataaat ttcagcgcct gttgattttc cggtaacagc gaaacatcct gcttgctgcg
      721 ctcctgacgc aatcgcgcca gcgttttctc cagtcgcgct tcatcaattg gcttcagcag
      781 ataatcaaag gcatgttctt caaaggcttt aatagcgtat tcgtcaaacg cagtgagaaa
      841 aacaatatac gggcgatgtt ccgggtcaag catccccacc atttccagac cactgatgcg
      901 cggcatctgg atatcgagaa acagcacatc cgggcgcagt ttatgcaccg cgccgatccc
      961 ttccacggcg tttgaacact ctccaacgat ttcaatatcg ctctgctcct gcaaaaatat
     1021 acgcaggttc tcccgtgcta acggttcatc atcgacaatt aagactttaa tcatgcctcg
     1081 tccctccatg gtagtcgtaa cgttattcgg gtgtaactat caggctcaca ggcgacgctt
     1141 attccatagt catcgccaaa ccgttcacgt aaacgcttat ccaccagatt catccccagc
     1201 ccactggcat tggttaccgg ttgatacaaa ccggcattgt cttcgatctc cagcatcaaa
     1261 tgttgcccct cacgtcgggc gctgattgcc actcgccctg tatccagcag ttgtgatgtc
     1321 ccatgtttaa tggcgttttc cactatcggt tgcagggtaa acgcgggcaa ttgctgctgg
     1381 gataattctt gcggaatagc aatgttgacc tgcaaccgcg actggaagcg cgccttttca
     1441 atttgcagat aagcgttcac atgttcaatt tcgtcggcga gagtaacaaa ctccgaaggc
     1501 cgctttaagt ttttgcggaa aaaagtggaa agatactgca ccagctggct ggcctgttcg
     1561 ctgtcgcggc ggatcaccgc tttaatggtg ttaagcgcat taaacaaaaa atgaggattc
     1621 acctgggcgt gaagcagttt gatttctgac tgggtgagca tcgctttttg ccgctcatat
     1681 tgccctgcaa gaatctgcgc cgaaagcagt tgcgcaatcc cctcgcccag cgtgcggttt
     1741 attgaactga ataaccggtt tttggcttca tacaatttga tggtgcccat cacgcgctga
     1801 ttttcgccac gcaacggaat taccagcgtc gaccccagtt tgcattgcgg atgcaaagag
     1861 caacgataag gtacttcgtt gccatcagcg tagaccactt caccggtttc aatcgctttt
     1921 aaggtgtaag tcgaagaaat cggtttgccg ggtaaatggt ggtcgtcacc aattccggta
     1981 aaggccagca atttctctcg atcggtaatc gcgactgcac caatatccag ctcctgatac
     2041 agtacctgag ccactttcat gctgttcact tcgttaaacc cctgtcgcaa aatgccttcc
     2101 gtcgaggctg ccactttcag cgcagtggca gaaaaagccg aagtgtattt ttcaaacatc
     2161 gcgcgtttat cgagcaatat acgcataaac agcgccgcgc cgacggtatt ggtgaccatc
     2221 attggcgcag caatattact caccagacgc accgcatctt cataaggtcg ggcgatcgca
     2281 aggatgatca gcatttgcac catttcagcg acgaacgtga cggcaccggc ggtaatgggg
     2341 ttaaagactt tatcagtgcg cccgcgacgg atcaggatgc tgtgtaccag gccgccgagc
     2401 aatccttcaa cgatggttga gatcatgcaa cttagcgcgg tcatgccccc catcgaatat
     2461 cgatgtaagc cgccggtcag accaaccagc ccaccgacga ccggaccgcc gagtaagccg
     2521 cccattaccg cgcctatcgc acgggtattg gcaatagaat cgtcaatgtg caacccaaac
     2581 caggtgccca tgatgcagaa gatggaaaag acgatgtagc agagaaattt atgcggcaga
     2641 cgaaccgtga cctgcattaa cggtatgaat aatggcgttt tactcattaa ccacgcaatg
     2701 actaaaaaaa cgcacatctg ctgaagcagc agcaacacca gattaaaatc gtacataccc
     2761 gcaaaccaca cttcccttta aaacgcgtaa catacattgc ctgcgtttaa ctttctttga
     2821 actcttgcag aaaaatgaga attcgtgagt acgatcactc aaaatcgcct ggcaaaaata
     2881 aaatcaccct atag
//
LOCUS       DAZSIO010000287         2889 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZSIO010000287 DAZSIO010000000
VERSION     DAZSIO010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.292"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..779
                     /gene="lsrF"
                     /locus_tag="ACHX5T_004078"
     CDS             <1..779
                     /gene="lsrF"
                     /locus_tag="ACHX5T_004078"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFR8469031.1"
                     /translation="WGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIAP
                     LFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNS
                     CAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRI
                     AAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVD
                     MGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            803..1093
                     /gene="lsrG"
                     /locus_tag="ACHX5T_004079"
     CDS             803..1093
                     /gene="lsrG"
                     /locus_tag="ACHX5T_004079"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFR8469032.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1133..1891
                     /gene="tam"
                     /locus_tag="ACHX5T_004080"
     CDS             1133..1891
                     /gene="tam"
                     /locus_tag="ACHX5T_004080"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFR8469033.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1895..2809)
                     /locus_tag="ACHX5T_004081"
     CDS             complement(1895..2809)
                     /locus_tag="ACHX5T_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFR8469034.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          760 a          667 c          771 g          691 t
ORIGIN      
        1 attggggaat gcagtcacgc ttatcgtgga tatttaatcc gaaaacgggt aaaactgtga
       61 tgctggcctt tgaccatggt tattttcagg ggccgactat cggacttgaa cgcattgata
      121 taaatatcgc cccgctgttt gaacatgccg atgtattaat gtgtacgcgc ggcattttgc
      181 gcagcgtagt tcccccggcg accaataagc cggtggtact gcgggcgtca ggtgcgaact
      241 ctattctggc ggaattaagt aatgaagccg tggcgttatc gatggatgat gccgtgcgcc
      301 tgaacagttg cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa catcagtcga
      361 tcaaaaatat tattcagctg gtcgatgccg gaatgaaagt gggaatgccg accatggccg
      421 tgactggcgt gggcaaagat atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa
      481 tcgccgctga aatgggggcg caaattatca aaacctatta tgtcgaaaaa ggttttgaac
      541 ggattgttgc cggatgtccg gtacccattg ttattgctgg cggtaaaaaa ttaccggagc
      601 gcgagacgct ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt gtggatatgg
      661 ggcgtaatat tttccagtct gaccatccgg tggcgatgat gaaagccgta caggcggtgg
      721 ttcaccataa cgaaacggct gatcgggcat atgaactcta tctgagtgaa aaacagtaac
      781 tgcggattta aggagaagaa ttatgcacgt cacactggtt gaaattaacg ttcatgaaga
      841 caaggttgac gagtttatcg aagtttttcg ccagaaccac ctgggctctg tacaggaaga
      901 aggtaatttg cgcttcgatg tcttacagga cccggaagtg aattcgcgct tttatattta
      961 cgaagcctat aaagatgaag acacagtggc attccataaa accacgcctc actacaaaac
     1021 ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg
     1081 gttgatgccg tgaggctaca attggggtaa cgcgctgacg ggagtaaaaa aaatgtctga
     1141 ctggaacccc tctttatatc tgcacttttc cgctgaacga tcgcgtccgg cggtggagct
     1201 gcttgccaga gtgccgctgg agaatgtcga atatgttgcc gatcttggct gtggcccagg
     1261 taacagcacc gccctgctgc atcaacgttg gcctgcggcc aggataacag gcatcgactc
     1321 gtctccggcg atgattgctg aagcgcgcag tgctttgcca gactgccagt tcgtggaagc
     1381 agacatccgc aactggcaac cggaacaggc actcgatctg atttttgcta atgcctcact
     1441 gcaatggctg cccgaccact acgaattgtt tcctcacttg gtttctttac ttaatccgca
     1501 gggcgtgctg gcagtacaga tgccagataa ctggctggag ccgacccatg tgctcatgcg
     1561 cgaagttgct tgggaacaaa actacccgga tcgcgggcgt gagccgttgg ctggcgttca
     1621 tgcttactac gatattttga gcgaagccgg atgtgaggtc gatatctggc gaaccactta
     1681 ctatcaccag atgccgtcgc accaggcgat tatcgattgg gtgaccgcca ctggattacg
     1741 tccgtggtta caggatctga ccgagagcga acagcagctt tttcttacgc gctaccatca
     1801 gatgctggaa gagcagtatc cactgcaaga gaacggacag atactgctgg catttccgcg
     1861 tctgtttatt gttgcccgcc gtacggagta attatcacgt cagctggtaa tgacgatcgg
     1921 gaagaatttt tgctggaatt tctgcttcat cgttcatctg taacaggtca atttcaatag
     1981 cgttgcagat ggcatccagc ggtaaatcat tgttttcagt accgaacgga tcttccagtt
     2041 cttccgccag acaatccagc gaaataaaag tgtaggaaat cagcacagag ataaaaggcg
     2101 tcatgtaatg caggtccacg accagcgcga acggcagcat gatacaaaac agataaacgg
     2161 tacgatgcaa aatcagggtg taggcaaagg gaattggcgt gtaggcaatg cgctcacagc
     2221 ctgccagaac cgctgaaata tcattaagcc gatcgttgag gctaataaac aggatatctg
     2281 aaagctgtcc attgcggcgc tgaaccgcca accattctcc cattattaac aagatacggt
     2341 tagctggaga gttcgaagcc agtacacgct gaagatctcc agtcttgaga taatgagcca
     2401 gcacttccgc ctgtggctgt ttgcgtaatg tcatgcgtaa acagtgggcg aaggcgattt
     2461 gcagcctggc aaactccctt acacttgccg aatccggcaa tgtcgttttt acctctcgca
     2521 gtaacgaccg tgaggcaatc atcaactgcc cccaaagttt tcgcgcttca acgtaacggg
     2581 cgtacccggc attattacga aaaccaagaa aaatggcgat ggcgacaccg agaatgctga
     2641 acggtgcgag ggtgaatttg atgcccagat gcgtgtacca gggcagcatg aatataacag
     2701 caatagaaaa gagaaaattg agtagtaagc gcgaggatat cttggataat actgagccgt
     2761 gccagacaaa aatacggcgc agccagtgtt gttgtggacg aacaatcatg gttatcttca
     2821 ggcgtggaaa agtcgcccta ttaaacgtga ttacgatcac attctcaaga cgctacttac
     2881 aaattacct
//
LOCUS       DAZSIO010000288         2862 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZSIO010000288 DAZSIO010000000
VERSION     DAZSIO010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.293"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..647)
                     /gene="yidZ"
                     /locus_tag="ACHX5T_004082"
     CDS             complement(<1..647)
                     /gene="yidZ"
                     /locus_tag="ACHX5T_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR8469035.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
     gene            complement(622..1797)
                     /gene="mdtL"
                     /locus_tag="ACHX5T_004083"
     CDS             complement(622..1797)
                     /gene="mdtL"
                     /locus_tag="ACHX5T_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR8469036.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(1929..>2862)
                     /gene="tnaB"
                     /locus_tag="ACHX5T_004084"
     CDS             complement(1929..>2862)
                     /gene="tnaB"
                     /locus_tag="ACHX5T_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFR8469037.1"
                     /translation="SANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAAS
                     RITSLFLGLKIISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFG
                     FHGNIPSLIICYGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGG
                     NIDSLVKSFLGTKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGR
                     FKTVLLTFLPPALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFT
                     VWGGNLIPAIVILFGITVILCWFGNVFNVLPKFG"
BASE COUNT          833 a          745 c          727 g          557 t
ORIGIN      
        1 caggtgtcgc tctgttccca gcaaatgctg atatgcggat aacgtaaaaa ggtgtccaga
       61 ttccacgttt catgcagtgc cggatgatct ttgcgtaacc agacgcaggg tacatcacta
      121 aacagcactt cataatcaat ggctaacggt agcgagctta acagctcccg cgagcgagga
      181 tggctttcgc gaccggaaaa accaatatcc acttcaccac gagtaatggc atctaaggaa
      241 tcgtaatccc agttacgtag tttgatggtc gcctgcgggt aacgttggta gatccgtttc
      301 gacagcgcat taagcatgat catcatcagc ggcgattccg ccgccaactc aaacttcagg
      361 ccgcgcggtg tctggtggtg cggtttatcc agcagcagat tgctcatttg catccattcc
      421 gccagatttt gctccatgct gaccattagc tgtgtgggcg acagccccag cggtgagttc
      481 acaaagagcg ggtcgtcaaa ccacgctctt aactttgcca gcgacttact caccgctgaa
      541 ggggtcacgt tcatccgctt cgccgctttg gtcacgctgc gctcctgcat cagcagttgc
      601 agacagagca gcagattgag atcaagcgtg gtgatggatt tcttcatgag cggcaacggg
      661 gcgtccaggc gcgacgaaca taatcagcaa caggctcact atgctacagg caatcagaat
      721 cccgatcagc atattccatg cgccgatacc aaccaccgcg gccagccaaa tccacagtga
      781 cgaaccgcaa acctgcgcaa tacctaaggt cgagctggct acgcccgcgc gtaatgaaaa
      841 tggtcctaac gcctgactca tcgctacgcc aaaacctacc gagaaaccgg cgcaaatcag
      901 cgtgatacca aacagagaaa ccgcatggga aggtgaaacg gcaagggtga tccccgccgc
      961 aaggaataac acctgcgagg tgatcatcaa cgtacgtggc ttaaaaattc ccagcgcaaa
     1021 tggcgtcgag aatgaaaagg tcatgctgac gccagcggtc agcgccataa tggtggcgta
     1081 ttctccgcgc tcaaacccca tgatttccat cagcaatacc ggcgatgtat tgacgaaagt
     1141 gaggatcacc gaaacgctga gggtggtgat aacaacacgg ctgaggaaaa aacgattaag
     1201 cagcgactcg ctattttctc gtggtttgtc cgaagcagcg ggggacgctg ggcgcgtttc
     1261 ttttaaaata aacaaagaca acatcagtac cgcgatgccc atcattgcca tcgcccagaa
     1321 cagactctgc cacgggaatt taagcataat cagatgtccg agcactggcg ctaacaccgg
     1381 aatgatgcag gtaataccgt tgagtaatga cagcacttta gcccgacgtc gatcatccag
     1441 cgtgtcgcgc aaaatggcga acgccaccac gtaacaacag cctgcgccca acccctgtag
     1501 aaatcggcct gcaagaaata acgtgctggt ttcagccagt gaacagaaca ccgaggcaat
     1561 aataaatagc gccgcgccgg gtatggcgac cggctttctc cctgaacgat cggccacttt
     1621 accggcaaat agcatcgcag ctgccatccc cgccagatat acggagaacg caatatgcaa
     1681 ctgcgcttcg ctggcattga gatcggcggc gatgcgcggt aaaccaacga ggtacatatc
     1741 aatcccggcg ggataaagta aaaccagggc aaaactacaa atcaaaaagc gggacatggg
     1801 ctaaagcgtc ctgatagtaa ggtgacgcaa agcatacgtg gtgaaggagg ataaggcgag
     1861 ttgccatttg gacataagtg atttcctgag aggcaagaag ccagcgaatg gctggcttct
     1921 tgaaggattt agccaaattt aggtaacacg ttaaagacgt tgccgaacca gcacaaaatc
     1981 acggttatac caaagagaat gacaatcgcc ggaataagat tgccgcccca gaccgtgaac
     2041 atctgattgg gaaacttctt gcgagctttg attgcaagca ctgcgggaat aatgaccgcc
     2101 cagatggtgg cgcacagccc ggcaccgccg atcccgtaaa taaagccgtt cgggaagatc
     2161 agatacaaca acgcaggtgg caggaaggtt aacagcacgg ttttgaaacg cccgccgtgg
     2221 gagttatcaa tcttaaacag gtccgccaga taatcgaaca accccagcgt gacaccaaag
     2281 aacgaactgg caacagctaa gttagagaac accagcaggc aaaactcgat aatgccgtgc
     2341 tgtttggtgc cgaggaacga tttcaccagc gaatcaatgt tgccgcctga ggagattatc
     2401 gccttaaagc tttcgcgcgg aatattcccc atcgtgcaat agagccagaa gagataaatc
     2461 accagcgcca gcagcgaacc aaataccacg cttttgatta acttatcttt gcgtttgcca
     2521 tagcaaataa tcaggctggg aatattgccg tggaaaccaa atgacgccag acacaccggc
     2581 aaagccataa agatatacgg gaagtaagac gttcccgcag tggtgctggt ggcatcgcgc
     2641 aggatggagt aatcgacctg gaagaagaaa gaaccaaaca cgatcacaaa ggagataatc
     2701 ttcagcccga ggaacaatga ggtaatgcga ctggcggcta acgaacttat ccacaatacg
     2761 ctggcaacga aaatggctgt gcagatcccg acaatacgtg gattagcgtg atagcccaaa
     2821 ttcattgata tcgtttcact aatgatcgca ccattagcag ag
//
LOCUS       DAZSIO010000289         2847 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZSIO010000289 DAZSIO010000000
VERSION     DAZSIO010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.294"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACHX5T_004085"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACHX5T_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR8469038.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKSSSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="ACHX5T_004086"
     tRNA            complement(621..695)
                     /locus_tag="ACHX5T_004086"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="ACHX5T_004087"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="ACHX5T_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="ACHX5T_004088"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="ACHX5T_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR8469039.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          684 a          697 c          686 g          780 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagacttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggac
//
LOCUS       DAZSIO010000290         2814 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZSIO010000290 DAZSIO010000000
VERSION     DAZSIO010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.295"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..165
                     /locus_tag="ACHX5T_004089"
     CDS             <1..165
                     /locus_tag="ACHX5T_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="HFR8469040.1"
                     /translation="ALERADRTLEPVIQRELEIREERLIQEREHALFLERERQPEIQE
                     RTLDGPSLGW"
     gene            complement(604..996)
                     /locus_tag="ACHX5T_004090"
     CDS             complement(604..996)
                     /locus_tag="ACHX5T_004090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469041.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
     gene            complement(1313..1471)
                     /locus_tag="ACHX5T_004091"
     CDS             complement(1313..1471)
                     /locus_tag="ACHX5T_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR8469042.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWTRWIEI
                     LRVLEVVA"
     gene            complement(2073..2264)
                     /locus_tag="ACHX5T_004092"
     CDS             complement(2073..2264)
                     /locus_tag="ACHX5T_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR8469043.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            2329..2652
                     /locus_tag="ACHX5T_004093"
     CDS             2329..2652
                     /locus_tag="ACHX5T_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFR8469044.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            2642..>2814
                     /locus_tag="ACHX5T_004094"
     CDS             2642..>2814
                     /locus_tag="ACHX5T_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="HFR8469045.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVL"
BASE COUNT          615 a          655 c          809 g          735 t
ORIGIN      
        1 gcacttgagc gagcagacag aacgcttgag ccagttatac agcgagaact ggagataaga
       61 gaggaacggt tgatacagga gcgcgaacat gcgttattcc tggaacggga acgccagccg
      121 gaaatacagg aacggacact ggatggccct tcgctgggat ggtaatcaga gacaggctaa
      181 aatgttgtca gcgcgtttcc ggtgcatttt ttgggataat ggttggttcg ggggcaccgg
      241 gaacgcgctt cagtcagacc ttgctgaaaa cacaggaacg agttaaaacg catacgggcg
      301 cgattctggt gtgtcttttg tcgctgtgtt ttgcctctat tgccagatcg cgctcattcc
      361 cctgaacttc tgttttcccg ctcaggtccc cgtcttcttc gcttccgaaa gcatgaataa
      421 aatccgtttc agctgtctct ggctggcaat tcctgcgtga ttcacatggc tgcatagcta
      481 tgcatgtggt taaaatttac cgggtgcgat cgcggcagtt tttcgggtgg tttgttgcca
      541 tttttacctg tctgctgccg tgatcgcgcc gaacgcgttt cagcggtgcg tacaattaag
      601 atattacgtt aaattgtgag ggaatggtcc ccatatactg actttatcat agggataggg
      661 ttggtagtgg gtcgattggg tttcggcatt gtttccgtta atgccaattg catttttgta
      721 tttgatgcaa cagaaggcgt gagattctcc tgggcctgga aaggctaagt gtttagaatt
      781 gatggcaaaa tatcttccgt ccgatttttg aagtagttgt tgtttggctg tctcccaaag
      841 ggtgctgtta caaacttctt gaatttgtag tttttgcagg actcttatta ctgcgttgtc
      901 atgttccaat tctttagggg agtgtattat tctctctttt aataagctgt ttaccacagg
      961 gtttagttgt tgtctaaagc acgcagataa tgccattgct acacaatttt gttcccgagg
     1021 aagcatgttt ggcggggtat gccgtgtttg atgtgatatg tctattctca tagaaggtct
     1081 ctggcttata gcatcaacaa atacggctat aaaacaaaat tttcatagag tgattcaaga
     1141 tcttcttgag atcctttttt gcggcgcgta atctgctgcc tgcaaacaaa aaaaccaccg
     1201 ctaccaacgg tggtttgttt gccggaatct agagctacca actctttctt ccgaaggtaa
     1261 ctggcttggt ggagcacaga taccaaatgc tgtccttcta gtgtagccgc agttaggcca
     1321 ccacttcaag aactcttaat atctcaatcc accttgtcca gttaccaatg gctgctgcca
     1381 gtggcgttaa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     1441 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1501 ccgaactgag ataccaacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     1561 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     1621 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     1681 gtcggttttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc atcggcgcgg
     1741 ccttcttcct gtttctggtc ttttgctcac atgttctttc cggcgttatc ccctgattct
     1801 gtggataaca gcatttcgtt tttgagtgag tagataccgc tcgccgcagt cgaacgaccg
     1861 agcgtagcga gtcagtgagc gaggaagcgg aaaagcgcct ggacgtgcat tttctcctta
     1921 cgcatctgtg cggcatttca cacccggtat ggcgcacttt tcatacaatc cgcactgatg
     1981 ccgcatggtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
     2041 ccgacacccg ctaacacccg ctgacaagcc cgtcagaggt tttcaccgtc atccccgaag
     2101 cgagcgaggc aactgcggta aagctcatcg gcgtggtcgt aaagcgattc acagatgtct
     2161 gcctgttcat ccgcgtccag ttcgttgagc ttctccagca gcgttaatgt ctggcttctg
     2221 ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa
     2281 gggggatttc tgttcatggg ggtaatgata ccgatgaaac gcgagaggat gctcacgata
     2341 cgggttactg atgatgaaca tgcccggtta ctggaacgct gtgagggtaa acagctggcg
     2401 gtatggatgc ggcgggtctg cctgggtgag ccggttgccc gttccgggag gctgccgaca
     2461 ctggcaccgc cgttactgcg tcagctggcc gccatcggga ataacctgaa tcaggcagcg
     2521 aggaaggtga acagcgggca gtggtcttcc ggtgaccggg ttcaggtggt ggccgcactg
     2581 atggccatcg gggatgagct gcgccggctg cgtctggccg tcagggaaca ggggacgcgg
     2641 gatgatagtt aagtttcatg ccaggggaaa aggcggtggc agtggtccgg ttgattacct
     2701 gctgggaagg gagcgtaacc gggaaggggc aacggtgctt cagggtaatc cggaagaagt
     2761 ccgggaactc atcgatgcca cgccatttgc gaagaaatac acgtccggtg ttct
//
LOCUS       DAZSIO010000291         2800 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZSIO010000291 DAZSIO010000000
VERSION     DAZSIO010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.296"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..593)
                     /locus_tag="ACHX5T_004095"
     CDS             complement(<1..593)
                     /locus_tag="ACHX5T_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR8469046.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQ"
     gene            complement(660..1385)
                     /gene="fimC"
                     /locus_tag="ACHX5T_004096"
     CDS             complement(660..1385)
                     /gene="fimC"
                     /locus_tag="ACHX5T_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFR8469047.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1422..1961)
                     /locus_tag="ACHX5T_004097"
     CDS             complement(1422..1961)
                     /locus_tag="ACHX5T_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8469048.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="ACHX5T_004098"
     CDS             complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="ACHX5T_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFR8469049.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT          723 a          705 c          608 g          764 t
ORIGIN      
        1 ttctgtacac tatttccgct gaaattataa ttgagcaatc cggcattaat accgggatcc
       61 cataactcag gaggaatata accacgcgcg cgattactca taaatgcctg agggatcgtc
      121 aggttcagtc gctgctgacc aacatccaga tgcgcagtag cgtcctggac cattgtggtt
      181 aatggcacac aggcatcatc cgccagcaga ttcataccgg cgacagaagc cgtattcagc
      241 cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa tcccttgttc actgtcgccc
      301 gtattaaatg tgacatcacg cgttgccata taaccattat tcaaatagat atcgacgcga
      361 tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg ataaatcggc cacagcctgg
      421 ggatcatccg ctaaaaagcg cagattaaaa tagaggtcgg cagatgacaa aggtgcctgt
      481 gcggcaaaag cacaggcgac aacgagtcgg acaaaaaaac cagccaaacg atgcttacga
      541 atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat tcagatatga cattattcct
      601 ccggtttaaa atcacccggc agtcaattct ttttattcag gcgaaaaatt ccccctgcgt
      661 tattccatta cgcccgtcat tttgggggta agtgcgccat aatcatttat tgttcggtaa
      721 gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc tttcgcccat tggaggcacc
      781 aatgcatttt caagaacccg ggttccggca ttcaactctg ttaccgtcag gtaatagggt
      841 gtcgggttaa tcagcgtcag agaattcgcg ctacgacgaa atcttaattt ttctgcggcc
      901 tgatcgggtg gcaacgctaa tttagccggg cgatagtaca gtttaatgcg gctgataatt
      961 gcgagctgta gcatattctc agtcaatttt gatttatcca ttgacggaat cgctttaacg
     1021 ttcatccaga ataaactttc ccggtcctgt ggcaattggt tatttgttgc atcaagaata
     1081 cgtaaggtat tctctttttt tcccttcatc gcaaacagag gaggcgtcac gataaaacga
     1141 ccatccttta caccatcggc attttccacc catgattgaa ttaaataggt actattttca
     1201 tcattatttg tcacggcaag ttgcacttgt ttttgccctg ccggataaat tacgcgagtc
     1261 gcacctaagg ccactcctgc ttcagcgcgt ccggcaacca tcattgccat gaacatcagg
     1321 atacctgcca gcaagcagaa tgttatttcc tgcgatttcc ttacattgac gtttttatta
     1381 ctcactgtcc tgttcctgtt atcacattat ctactgaaca attactgata ggttaaagag
     1441 aaccaggcct gggcattggc gatgccgcca gtaacccgac gcccggtagc acgatatttg
     1501 gcgatgaaat gtagcgaagt agagcctgag taaagccgtt tccagtttgc tggaggacga
     1561 ttaatcggta cgaggtttcc ttcatcatca aacaacgcta caccaatatt ggaggctatc
     1621 cctggcccct ctcccacgga gagcacatcc ggatttttac catccgcgac accgtgaaac
     1681 gccacaccca cacgttcact caccaccgtg ctacattccc gtaaatgaat aacaaaaggc
     1741 accggtgcgc tatcttcccc tgccgcatga aaccggttac tgctgatttg ccccatattg
     1801 accgtcattt gtttatcacc ggcttcaatc cggcaagttt ccgcaataat gacgccctga
     1861 aattgcatat ttccgccggg cagcgtggta ttccatttat ttccggccag agcaaacatt
     1921 ggcagcaacg acgctaacag aaataatctc ttccttttca tcgttatttt tatcgcacaa
     1981 gggtgggcat ccctgcccgt aatgacgtcc ttgaacctgg gtaggttatt gatactgaac
     2041 cttgaaggtt gcatccgcat tagctgcacc cggggttgcc tcgccgattg cataataacg
     2101 cgcctggaac ggaatggtgt tggtaccgtt attcagggtt gtttgtgcac taaatgtcgc
     2161 accgtccagc gtcagagcat tgcctgttct gtctaggatc tgcacgccaa cgtttgttgc
     2221 actacctgca gcagaactct gcagagccag tacatcagta cgcgtagcat caattgccgt
     2281 acctaagaag gcaacagcgg cttttgtggc aacagtggta tcgcaatcat tcagctgaat
     2341 gttaaaacca acggcagagc tggttgctcc agcctgcttc aggctagcgg tacgaacctg
     2401 tcccaactga acggtttgat caacagagcc tgcatcaact gcgcaagcgg cgttaacaac
     2461 ttccccttta aagtgaatgg tcccaccatt taccgtcgta gtatcggcca gagccgctgc
     2521 ggaactgagg gacagagccg acagaacaac gattgccaga gttttaattt tcatgctgct
     2581 ttcctttaaa aaaactattt ctaaatcgac atgggcagtc gttctgtaca ctttgttttg
     2641 tcaacgagtt tgactgccaa cactgcacag ttttccccca aaagatgaaa catttggggc
     2701 cattttgact catagaggaa agcatcgcgc acaaactttt tcagtttatt tgttggctta
     2761 atattctatt attatcttta tttatagatt tttatattgc
//
LOCUS       DAZSIO010000292         2789 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZSIO010000292 DAZSIO010000000
VERSION     DAZSIO010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.297"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..259)
                     /locus_tag="ACHX5T_004099"
     CDS             complement(<1..259)
                     /locus_tag="ACHX5T_004099"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469050.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARC"
     gene            complement(658..817)
                     /locus_tag="ACHX5T_004100"
                     /pseudo
     CDS             complement(658..817)
                     /locus_tag="ACHX5T_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(821..2050)
                     /locus_tag="ACHX5T_004101"
                     /pseudo
     CDS             complement(821..2050)
                     /locus_tag="ACHX5T_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2070..2417)
                     /gene="tnpB"
                     /locus_tag="ACHX5T_004102"
     CDS             complement(2070..2417)
                     /gene="tnpB"
                     /locus_tag="ACHX5T_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR8469051.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(2417..>2789)
                     /gene="tnpA"
                     /locus_tag="ACHX5T_004103"
     CDS             complement(2417..>2789)
                     /gene="tnpA"
                     /locus_tag="ACHX5T_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFR8469052.1"
                     /translation="RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQY
                     RKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRL
                     KGKLTPALLQILIREIKGSSH"
BASE COUNT          643 a          742 c          704 g          700 t
ORIGIN      
        1 tgcagcgggc aatttccgga tacccttcgt catcaacacg tacaaaccag aagaccagtt
       61 ttttgtttcc ggcatccaca aagaacggaa tattcaggtc tgcgcaacac tcaacggcat
      121 cgtcaaaatt atcaaagcgc agaacctcag cttcttcttc gtcaaaaaaa tcatcttcgt
      181 gaagttttac gacatagcgg aggagtttgc ttctttgagg tgcaggtttt cgtttgcgtg
      241 gtttagctga acgggccata taactacctg aaagacaatg atattgtctg tttttataac
      301 ggtaatcaca ggccatgaca agccgcagcc gtcaggccgc ctactcgggg cggtttagaa
      361 cgcttattta gtaccagatc ttacttataa taaaggggct tggcagtttg gataaactat
      421 tgtccttatt ccactaatac aaactttttg taaagtatat taaatatact tcatttcata
      481 ctgaaatccc cggaagtatt caatcaaaca attacaccat aaaaggaaga gggagcccca
      541 attaagttat caagcatgtt tatgatacct gcagggtata aattttctgg ctgcaagcag
      601 acacatataa aacgtcttta ttgatcctac ccacataata tggacacagt cgtaagtgag
      661 gctctggttt tcaaattgtt ccggactgag accaccacac caacggtgcc gtcgccagcg
      721 attgtaatcg cactcgatat aactaaacac tgttgcccgc actatttccc gatggataat
      781 agtgttctcc gtggagacat tccattttca gcgaatgaat tttcatctgt tgctgtatcc
      841 agtcatacag tgactgcatc agtggcgcgg ctctggcttt tcttgccgcc agacgctgtt
      901 ctgctgaaca tccccggact tctgcctcga tggcatacag ttcaccgata cgctgcaggg
      961 cttccgtggt gatgtcggtt ggcactcttg catgcacatc gtggattttt ctccggacat
     1021 gagccataca tgcggcttcc gttattctgc cggattcgta caacgcccgg taaccaccgt
     1081 aagcatcggc ctgaagcact ccgctgtaac cggccagatg attctgtgga tggatacctt
     1141 tccggtccgg tgagtacgcg aaccagaccg ccggaggcat ttccgaaccg gcgttacggt
     1201 catcacgaac gtagacccac agccgggcag tccttgtttt accgctgccc ggctcctgga
     1261 ccggaacggg gatatcatcg gcatggactt taccgggcat cagcacatac tggcgcagga
     1321 tgtcatacag cggttccagc agttcagcaa cagcacctgt ccagcgcccc agtgtggcac
     1381 ggctcagctc cacgccctga cgacggtata tttctgactg gcggtataac ggcagatagt
     1441 ctgcatattt tccagcaaca acatgggcca gaagccccgc tccggcataa ctgcgtgcaa
     1501 tgggttttga aggcaccggt gcctgcacga tatggtcaca ccggcaacag gccagtttcg
     1561 gacgttgtgt ttcgataacc ttaaaggcgc tgctgataag ctccagctgc tctgacacat
     1621 cacatcccag agaactgagt tcgccaccac aggccgggca gcattcctct tccggacgga
     1681 tgacccgggt ttcacgggga agtgaggctg gtaacggttt acgggctgaa gactggcgca
     1741 ggggagatgg cagtaccggg tcatattgct cacccagcgt ttccgccatt tcttcctgaa
     1801 gtgcgctgat tcgctcctgt gcttccagta tctgccgttc ggtttttgcg cgaagttttt
     1861 ctgagctttt accgaactgc atgcgctgca gtttcgcaac cagcgccttc agccggttga
     1921 tttcggaagc ataagccgcc acccgctgtg agagcaggcg gttgtatttc gccatctggc
     1981 ggatggtgtc ctgttgcgtc tgcaacagtg cccgcaggcg ggcattctca tgagcaagtg
     2041 aggtgtccat atcctcactt tacaacgggt tatatgcgga tgccagcgcg ttccgttcgt
     2101 ttcgggtgtt tccagtcgat gccctcaaga agcatggata actgggccgg agtaaggtgc
     2161 accttgccgt cacgggtgac cggccagacg aagcggcccc gctccaggcg tctggtaaag
     2221 aggcacagtc cgtcactgtc agcccacaac acttttatct ggtcaccccg gcgtccgcgg
     2281 aagatgaaca gatgcccgga gaacgggtca tccttcagga cgttctgaac ttttgagacc
     2341 aggccgttaa agccatttcg catatcggtg atacctgcaa ccagccagat acgcgaacct
     2401 gcagggaaag atatcatcag tggctgctcc cttttatttc gcggataagt atctgtaata
     2461 acgccggcgt cagtttacct ttaagcctga gagttccggc cggcagaacc agctcacagc
     2521 acagactgtc ggacggtgta tttatctgct ctggttcctg tgccggagcc gggattttat
     2581 tatccggctc cggcgttaac gtcacgggaa gcagtgcagg catatttttt ccggaaggca
     2641 gcaggccacc tttccggtat tgatggcgcc agttgaagag caggttatcg ttgattccgt
     2701 tttcacgggc gatctgcgcc acacaggctc cgggctgcag tgactgctcc actaaagcga
     2761 ttttaaattc ataaggaaaa ttaggccgc
//
LOCUS       DAZSIO010000293         2768 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZSIO010000293 DAZSIO010000000
VERSION     DAZSIO010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.298"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..98
                     /locus_tag="ACHX5T_004104"
     CDS             <1..98
                     /locus_tag="ACHX5T_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR8469053.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            156..512
                     /locus_tag="ACHX5T_004105"
     CDS             156..512
                     /locus_tag="ACHX5T_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469054.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            605..823
                     /locus_tag="ACHX5T_004106"
     CDS             605..823
                     /locus_tag="ACHX5T_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR8469055.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            825..1190
                     /locus_tag="ACHX5T_004107"
     CDS             825..1190
                     /locus_tag="ACHX5T_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR8469056.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            1187..1852
                     /locus_tag="ACHX5T_004108"
     CDS             1187..1852
                     /locus_tag="ACHX5T_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469057.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1852..2217
                     /locus_tag="ACHX5T_004109"
     CDS             1852..2217
                     /locus_tag="ACHX5T_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR8469058.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2672..>2768
                     /gene="hokD"
                     /locus_tag="ACHX5T_004110"
     CDS             2672..>2768
                     /gene="hokD"
                     /locus_tag="ACHX5T_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR8469059.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEV"
BASE COUNT          783 a          566 c          738 g          681 t
ORIGIN      
        1 ggcgcctgaa tataatctgt cgcgagtgcc ggaagagcga agctatgcag cgtgtactgg
       61 ctttctatca gggtaatttt caggaggcga tactgtgaat gaaattagct atcaggcttc
      121 aattaccgct ggcattcgca tcaaaggaga ggagcatgga aataaaacca gaagatgagt
      181 taagcaatat cgttttattt ccgataaaag aggatgaccc tcgtaatcag gttaattttc
      241 tttataagcc atcggaaaga ccatattgcc atcacgcctc tgtacgggtt gacgaaaaag
      301 agcgtcaggt ccgctgtaaa atctgcggtg cggttgtgga gccgtttgac tggatgctct
      361 ctgtggcaaa aagagaaacc agactggcag atgatgtaag gctattgcgc caggaggaac
      421 aggaaaggcg gagaaatata gaaaagctga tacagattga gcgtaacgcg aaagcgcgga
      481 tacgcagggc gacaaaatcc agaactgaat aattaaattt agcactgtta aaaatttaat
      541 ccttaaccgg agggatttct gcaccctcaa atcatcagga gaccgcccga aagggcggta
      601 atgaatggtt acattattta gaaaaaaata tccgcgaaag agtagaacaa cagaattcct
      661 gtttctcatt ctgtttatcg tgttgatgac accgatatcc ccgcaaattt ttgtctggat
      721 aatcggaaaa ataattgagc cagttattga attgtataac gacgtggtat gggcgtcatt
      781 caacacactg cacaataaaa ttaatccgta taaggaaagc tgatatggca ctgacgaaaa
      841 aacaacgtgc agaactgcgc atgaagttcg gtggtcgctg cgcttattgc ggctgcgaac
      901 ttggcgaaaa gtggcatgca gaccatgtaa aaccggtcat tcgttttgat ggaaatatgc
      961 ttcaccagga acgtgacgat atatccaaca tggtaccaga atgccaccca tgcaatctgc
     1021 acaagcattg cagtagcctg gaagattatc gacgaattat cagtgatggt cgtcgtgaat
     1081 tccttgcgtc cgggaaaggc aaagcgctgg ttcgtatggg attggttgaa atgaaatctg
     1141 acccggttgt gttctggttt gaaatatatc aagaaggggc tacggcatga ctactattac
     1201 caaagagcga ctgcaatggc tggctaacat ttctggccgc gatgatattg acgatataga
     1261 cggcggtgaa attcgtgaac tggcgcttat cgctctggca tcactggaag cagagcctgt
     1321 agcggaatgc attgttgaag atgggggcat gtgtattgat gggttcggtg agtatgtggg
     1381 tcactcgctg cctgatggaa tgcatgagct ttatgctgcc ccgcgaatgc gacaacctga
     1441 tggatggaaa gccgtagctg tagcgtggaa ggtgacgttt actcaagttg accaggaatc
     1501 taatacgttc actgctatat atttttacaa agcggaagtt gagcgttggg tacgactgca
     1561 taaagcatgt gattttcggg cagatataac accgctttac gcagcgccgg cagtgccggt
     1621 tgcagtaaac gacgacatgg cttacgcatt ccatcacgca ctgtcagatt catcgcttgg
     1681 ctctgatgaa atcgaagaaa ttaaaaccgg tttgcgtgct gcctttgcca acgtcactat
     1741 ccaaccagag ccgatagtgc cggatgaaat cgggccaaac gatagtaata cgtttgatta
     1801 tgttgatggc tggaacgcct gccgcgctgc catgcttaag ggagataaat aatgattaat
     1861 cgaaccaaac tggagcacat tctcgaatat gccaggcagc agaaatgtat tgggcaactt
     1921 tgtaaaattc caccaggaga tatggttgaa atcgtggaaa tggccatgcg taaggctggc
     1981 aactctccgg taactctggc tggctggata agctgtagtg atgcagttcc tgctgaatat
     2041 tgcgatgtga ttcttctcga tgatctcggg aatgtattcc ccggttcctg ggataaggtt
     2101 ttttgcccca ctcgtggcgg gaataagatg gcttttgtgg acaaagacgg cgttgaagta
     2161 gagagctcaa ctcactggat gccgctaccg gaaccaccgc aggaggtgaa tcaatgacct
     2221 ggcctgaggc attcaccacg gtaggaattg cgatggcggt ggcgctggtg gtgtattcga
     2281 tttgccgttg gggttaacaa acaaaaaccc cggattgatg gtccggggtt tttgaaggaa
     2341 acaaaacaga aacagcaatt gccgttacct gttgttacca tggcaagtaa acgtatctca
     2401 ggcgagcgca ttgcgccgtt ctgacgcaga taaattagcc tggatagaag gtgctggcaa
     2461 taaaaaatag cgttttctta tcggtatcgg taagattgct gcgggtgctt gaggctgtct
     2521 gcctcgggca tgccactgta aggcagacag agaaaagccc cagttaacat tacgcgtcct
     2581 gcaagacgct taacattaat ctgaggccca atctatgtct cacaaatgta ggttagcctc
     2641 ttacgtgccg aaaggcaagg agaagcaggc tatgaagcag caaaaggcga tgttaatcgc
     2701 cctgatcgtc atctgtttaa ccgtcatagt gacggcactg gtaacgagga aagacctctg
     2761 cgaggtac
//
LOCUS       DAZSIO010000294         2751 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZSIO010000294 DAZSIO010000000
VERSION     DAZSIO010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.299"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            41..1438
                     /gene="ccp"
                     /locus_tag="ACHX5T_004111"
     CDS             41..1438
                     /gene="ccp"
                     /locus_tag="ACHX5T_004111"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR8469060.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1649..>2751
                     /locus_tag="ACHX5T_004112"
     CDS             1649..>2751
                     /locus_tag="ACHX5T_004112"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR8469061.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICT"
BASE COUNT          708 a          623 c          722 g          698 t
ORIGIN      
        1 tttctgggtc acaataactc accaccgtga gggaagaata atgaaaatgg tctcacgtat
       61 taccgcgatc ggcctggctg gcgtcgcgat ttgctattta gggttatctg gttatgtgtg
      121 gtaccacgat aataaacgca gtaaacaggc cgatgttcag gcatctgctg tcagtgaaaa
      181 taataaggtt ttaggctttc tccgcgaaaa aggatgcgac tattgccaca cgccttcggc
      241 agaattaccc gcctattatt atattcctgg cgcgaaacag ttgatggatt acgacattaa
      301 gcttggatat aaatctttta acctcgaggc cgtgcgtgcg gcactgctgg ctgataaacc
      361 cgtttcgcaa agcgatctga ataagattga atgggtgatg cagtatgaaa ctatgccacc
      421 aacgcgttat accgcgctac actgggcggg taaggtgagt gatgaagagc gggcggaaat
      481 actggcctgg attgcaaaac agcgcgcgga atattacgcc agcaatgata ctgctccgga
      541 gcatcgcaat gaaccggtgc agcccatccc gcaaaaactg cctaccgatg cgcaaaaagt
      601 ggcgttgggc tttgcgctgt atcacgatcc ccgtttatcg gctgatagca ccatttcatg
      661 cgctcattgc catacgttga atgcgggggg cgtcgatggc agaaaaacat cgattggtgt
      721 tggtggcgca gttgggccga ttaatgcgcc gacggtattt aactcagtat ttaacgttga
      781 gcagttctgg gatggtcgtg cggcaacatt gcaggatcag gcaggtggac cgccgttgaa
      841 cccgattgaa atggcgtcga aatcctggga cgaaattatt gctaagctgg aaaaagatcc
      901 gcagcttaaa gcgcagttcc tcgaagttta tccgcaaggt ttcagtggcg aaaatattac
      961 tgatgccatt gctgaatttg agaaaacatt aattacgccg gattccccat ttgataaatg
     1021 gttgcgtgga gatgaaaatg cgctgacggc gcaacagaaa aaaggctatc aattatttaa
     1081 agataataaa tgtgcaactt gtcatggtgg tattattctc ggcggacgtt cctttgaacc
     1141 gttggggctg aaaaaagact ttaactttgg ggaaattacg gcggcggata ttggtcgtat
     1201 gaatgtgact aaagaagagc gtgataaatt gcgtcagaaa gtacccggtt tacgtaacgt
     1261 tgctttaacg gcaccgtact tccatcgcgg tgacgtgccg acgctggacg gggcggtgaa
     1321 actgatgctg cgctatcagg taggcaaaga gctgccgcag gaggatgtgg atgatatcgt
     1381 agctttcctg cacagtctga acggggtgta cacgccgtat atgcaggata aacaataatt
     1441 aatttgatcg cccgaacagg aatgtttggg cgatttttat tacgataata aagtctgttt
     1501 ttaatattat catgttaaat gtttatatta taaaaagtcg tttttctgct taggattttg
     1561 ttatttaaat taagcctgta atgccttgct tccattgcgg ataaatccta cttttttatt
     1621 gccttcaaat aaatttaagg agttcgaaat ggaccagaag ctgttaacgg atttccgctc
     1681 agaactactc gattcacgtt ttggcgcaaa ggccatttct actatcgcgg agtcaaaacg
     1741 atttccgctg cacgaaatgc gcgatgatgt cgcattccag attatcaatg atgaattata
     1801 tcttgatggc aacgctcgtc agaacctagc cactttctgc cagacctggg acgacgaaaa
     1861 cgtccataaa ttgatggatt tgtcgatcaa taaaaactgg atcgacaaag aagaatatcc
     1921 gcaatccgca gccatcgacc tgcgttgcgt aaatatggtt gccgatctgt ggcatgcgcc
     1981 tgcgccgaaa aatggtcagg ccgttggcac caacaccatt ggttcttccg aggcctgtat
     2041 gctcggcggg atggcgatga aatggcgttg gcgcaagcgt atggaagctg caggcaaacc
     2101 aacggataaa ccaaacctag tgtgcggtcc ggtacaaatc tgctggcata aattcgcccg
     2161 ctactgggat gtggagctgc gtgagatccc tatgcgcccc ggtcagttgt ttatggaccc
     2221 gaaacgcatg attgaagcct gtgacgaaaa caccatcggc gtggtgccga ctttcggcgt
     2281 gacctacacc ggtaactatg agttcccaca accgctgcac gatgcgctgg ataaattcca
     2341 ggccgacacc ggtatcgaca tcgacatgca catcgacgct gccagcggtg gcttcctggc
     2401 accgttcgtc gccccggata tcgtctggga cttccgcctg ccgcgtgtga aatcgatcag
     2461 tgcttcaggc cataaattcg gtctggctcc gctgggctgc ggctgggtta tctggcgtga
     2521 cgaagaagcg ctgccgcagg aactggtgtt caacgttgac tacctgggtg gtcaaattgg
     2581 tacttttgcc atcaacttct cccgcccggc gggtcaggta attgcacagt actatgaatt
     2641 cctgcgcctc ggtcgtgaag gctataccaa agtacagaac gcctcttacc aggttgccgc
     2701 ttatctggcg gatgaaatcg ccaaacaggg gccgtatgag ttcatctgta c
//
LOCUS       DAZSIO010000295         2734 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZSIO010000295 DAZSIO010000000
VERSION     DAZSIO010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.300"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            88..153
                     /locus_tag="ACHX5T_004113"
                     /pseudo
     CDS             88..153
                     /locus_tag="ACHX5T_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            381..629
                     /locus_tag="ACHX5T_004114"
     CDS             381..629
                     /locus_tag="ACHX5T_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR8469062.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(745..1014)
                     /locus_tag="ACHX5T_004115"
     CDS             complement(745..1014)
                     /locus_tag="ACHX5T_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR8469063.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            1071..1739
                     /locus_tag="ACHX5T_004116"
     CDS             1071..1739
                     /locus_tag="ACHX5T_004116"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR8469064.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            2055..>2734
                     /locus_tag="ACHX5T_004117"
     CDS             2055..>2734
                     /locus_tag="ACHX5T_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8469065.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          835 a          589 c          538 g          772 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacacgtaa aatcggtaac ggctggaaat
      661 cattcaatac tcgcactatc gaaagtttac cagccaaccg cagcacgtcc tgcatacgtc
      721 gtgtctgcgg tttttctttt tcgcttacat tgtgtctggt tcttccggcc actcaatatc
      781 aggtgcagtt gatgtatcaa cacggttcag caacacccga tacttcttcc aggcttccag
      841 caacgagttt tcttcctccg ttgcgatctc cagatctaca gcatcctgaa gtggcgcaat
      901 atgctcactg aatccctgga tgtagaactg tgtggtgacg gtcttccagc cattcggctc
      961 ctgctgtatc gaagcatacc aggctatttc aatatcgcta tgctgcggca gcatttaacc
     1021 ccttgtaatt catcgccata attgatttaa ttcacaaata aaactataat atggtgaaat
     1081 taatgaaaaa aaacacagat gatggggcta aaatttacac accacttacc ctaaagcttt
     1141 atgactggtg ggttttggga gtatcaaatc ggcttgcatg gggatgtcct acaaaggaac
     1201 accttcttcc acactttctg gaacatttag gtaacaacca tctggatatt ggtgttggaa
     1261 ctgggtttta ccttactcac gtacctgaga gtagtctgat atctttaatg gatttgaacg
     1321 aagctagcct gaacgcggca tctacaaggg ctggggaatc aaaaattaaa cataaaatta
     1381 gccatgatgt ttttgaacct tatcctgcgg cgttacatgg tcaatttgat tccatttcca
     1441 tgttttacct tcttcactgc ctgcctggaa atatatctac aaaaagctgt gtaatacgca
     1501 atgcggcgca ggccttaact gatgatggaa ctctatacgg agccacaatt cttggcgatg
     1561 gagttgtgca caatagcttc ggtcaaaaac tgatgcgcat ttacaatcag aaaggcatct
     1621 tttcaaacac aaaagattcc gaagaaggct taacacatat actctcagag catttcgaga
     1681 atgttaaaac caaggttcaa ggtactgtag taatgttttc cgcttcaggg aaaaaatagc
     1741 atccaaccgc agcacgttct tgcttaagac gtgctacggt ttcctttacg atttggggtt
     1801 gcgactttac ctctattgat aatgcattcc ggcagaacgt tcaaatatga acgtacgata
     1861 tttaactaac cgaaaaacaa aatatagcac aggcgagaca tttaccatct aagaaaatag
     1921 tatcgttttt ttacagttaa atcaacatca cttccttaaa atgaaaacaa taaatttcaa
     1981 tcctgaaaca ttttatttca gcttattatt ccccagtatc acattttatc aggatatcct
     2041 tctgcgaggt tattatgctt cctgtaaata atccccccct atccactgga aacgtctctt
     2101 tttacagaac tacatcaatc gacaatgttc acaataatta tctctccgaa tgggttgaat
     2161 ggactaaaaa cagcatttcc ggagaaaaca gggaaactgc ttttacccgg ctccaattat
     2221 gtctggagaa cagtgaaaca tcgttggact tatcttgttt aggtctcaga tctctaccac
     2281 gattgcctga caatcttgat gaaattaatg taagcaataa ccaactatca atgctccccg
     2341 agctaccaag ggcattgaaa gagctgaatg caagcagtaa tcaattatct gcacttcctg
     2401 aattaccagt gtcgctggaa tatataaatg tgagtgataa ccatttgttc gcacttcctg
     2461 aattacctgc gtcactagaa tatattaatg taagtgacaa tcacctgtct gtacttccga
     2521 ggttaccaat gtcattggaa ttacttgatg cagccagaaa tgctttggaa gtaataccag
     2581 attttccaga aagagatgat catattataa gaatattctg gcttaatcag aaccggatca
     2641 cggcaattcc ggaaagcata cttggcctca gttctgatag cgttgtcaat cttagagaaa
     2701 atcaactatc tcccagaata atgcaaactt tgtt
//
LOCUS       DAZSIO010000296         2718 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZSIO010000296 DAZSIO010000000
VERSION     DAZSIO010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.301"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..197
                     /locus_tag="ACHX5T_004118"
     CDS             <1..197
                     /locus_tag="ACHX5T_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR8469066.1"
                     /translation="LVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQ
                     RGVNVFIQDVPGDQKEQIPD"
     gene            236..1039
                     /gene="agaC"
                     /locus_tag="ACHX5T_004119"
     CDS             236..1039
                     /gene="agaC"
                     /locus_tag="ACHX5T_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFR8469067.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1029..1820
                     /gene="agaD"
                     /locus_tag="ACHX5T_004120"
     CDS             1029..1820
                     /gene="agaD"
                     /locus_tag="ACHX5T_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFR8469068.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1821..2576
                     /locus_tag="ACHX5T_004121"
     CDS             1821..2576
                     /locus_tag="ACHX5T_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFR8469069.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          675 a          629 c          677 g          737 t
ORIGIN      
        1 aattggtaga aggtggcatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg
       61 aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc gcggacttac
      121 gttttattaa acaacgtggc gtgaatgttt tcattcagga cgtccctggc gatcaaaaag
      181 aacaaatccc tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa taaaaatgca
      241 tgaaataacc ctacttcagg gattatccct agcggcgtta gttttcgttc tggggattga
      301 tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc taactggcgc
      361 tattctcggt gatattcaga ctggcttaat taccggtggt ctgacagagt tggctttcgc
      421 cggattaacc cctgcaggtg gtgttcagcc gcccaacccg attatggcgg gtctgatgac
      481 caccgtcatt gcatggtcta cgggcgttga tgccaaaaca gcaattggtc ttggcctgcc
      541 gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct cattatttat
      601 gaccaaagcc gataaatgcg cgaaagaggc ggatacggca gcgttttccc ggcttaactg
      661 gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct gtacttacct
      721 ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggttga cccacggctt
      781 tgaagtggct ggcggtattc tgcctgccgt tggttttggc ttgctgctgc gcgtaatgtt
      841 caaagcgcaa tatatccctt acctgatcgc cggtttcctg tttgtttgct acatccgggt
      901 cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg agtttttcaa
      961 tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg aagaagagga
     1021 ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc tttcgttcgt
     1081 cgctgctgca agcgagcttt aactacgaaa ggatgcaggc gggcggtttt acctgggcga
     1141 tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcttaagc gcggcgatga
     1201 aagataacct cgaatttatt aatacccacc cgaatctggt cggattcctg atggggttat
     1261 taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc aaagtggcac
     1321 tgtttggccc aatcgccggg attggcgatg cgattttctg gtttactttg ttgccgatta
     1381 tggcgggaat ttgctcatca tttgccagcc agggaaacct gctggggccg attctatttt
     1441 tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc ggttattcag
     1501 tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca gatgattgct cgctcggcaa
     1561 ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat attaacgtgg
     1621 tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct gcaacaggat ttcttcgata
     1681 aagtcttccc gaacatttta ccgatggcct acaccctgct gatgtattac ttcctgcggg
     1741 tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct attgtttgtt
     1801 ccgcattcgg cattttgtaa atggaacgag gcactgcttc tggcggtgcc tctttactta
     1861 aggaatttca tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg ttaagtgaac
     1921 gagccagcga atatttattg gctgtgatcc gtagcaaacc ggatgccgtg atttgcctgg
     1981 cgaccggagc cacgccatta ctgacgtatc attatctggt agaaaaaatc caccagcagc
     2041 aggttgatat cagccagctc accttcgtga agctcgacga atgggtggat ctgccattaa
     2101 cgatgcctgg cacctgcgaa actttcctac aacagcatat cgtgcagccg ctggggctac
     2161 gtgaagacca gctcatcagc tttcgctccg aagagataaa tgagacagag tgcgaacggg
     2221 taacgaacct gattgcgcgc aaaggcggtc tggatttatg cgttctcgga ttggggaaaa
     2281 acggtcatct tgggctgaac gaaccgggag aaagcctaca accggcctgc catatcagtc
     2341 aacttgatgc cagaacacag caacatgaga tgttaaaaac cacgggtcgc cccgtgactc
     2401 gtgggatcac cttaggcctg aaggatattc tcaatgcccg cgaggttttg ttattggtga
     2461 ctggcgaagg aaagcaggat gcgacagaac gttttctcac ggctaaagtc tccaccgcta
     2521 tcccggcttc atttttatgg ctgcacagta actttatttg tttaattaat acctaaccag
     2581 atgaaaaatt tgctcaaaat ttttaaatta acttattcaa cagtcacgca ttatattaaa
     2641 taacatttga ctgggttgaa cataacgcca atagcaaaag gagttatgtt caacaattta
     2701 attttagata tacatata
//
LOCUS       DAZSIO010000297         2712 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZSIO010000297 DAZSIO010000000
VERSION     DAZSIO010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.302"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            50..2572
                     /gene="lpfC-O113"
                     /locus_tag="ACHX5T_004122"
     CDS             50..2572
                     /gene="lpfC-O113"
                     /locus_tag="ACHX5T_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR8469070.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
     gene            2583..>2712
                     /locus_tag="ACHX5T_004123"
     CDS             2583..>2712
                     /locus_tag="ACHX5T_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8469071.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          726 a          585 c          678 g          723 t
ORIGIN      
        1 tgacccgata aaaatgacgc tgtaagcaag ggataaggat aggcgtatta tgatgacgac
       61 cagaatagtg gttggcctca cggcagggac gtgtctgatt ttctcgcaaa acctgatggc
      121 cgaggtcagt gtattcaatc cggcgcttct ggaaatcaac catcaatccg gagtcgatat
      181 tcgccagttt aatcgggcaa acctgatgcc cccaggtgtt tatagcgttg atatttttat
      241 caacggtaaa atgtttgaac gtcaggatgt gacatttgtt caggataatc cagatgctga
      301 tctgcacgct tgctttattg ccattaaaaa aacactgtcc tcctttggca taaaagttga
      361 tgcgctcaaa tcgttcaatg atgtggatga gacggtttgc ctcgatcctg ctccacgtat
      421 tgaaggctca tcctggcagt ttgacagtga taaattgcag ctgaatatat ccattcacca
      481 aatctacatg gacgcgatgg cttatgatta catcagcccc acgcgttggg atgaggggat
      541 taatgcgctc accatcaact acgatttttc tggttcacat acactacgtt cagattatgg
      601 ttcacaagag acagatacca gttatctcaa tctgcgcaat ggactgaata ttggaccgtg
      661 gcggctacgt aattacagta ctttaaacac cagcgatggc cgtgcggaat acaactccat
      721 tagtacctgg atacagcgcg atattgccgc gttaagaagc cagattatga ttggtgatac
      781 gtggacggcg agcgatattt tcgacagtac gcaaattcgc ggcgcgcgtt tgtatactga
      841 taacgatatg ctacccgcca gccagaatgg ctttgctcct gtggttcgtg ggattgcaaa
      901 gtccaacgcc accgtcatca ttcggcagaa tggctacgtg atttatcagt cagccgttcc
      961 acaaggtgct tttgagatca ccgatctcaa caccgcaagt acaggtggcg atttggacgt
     1021 aaccatcaaa gaagaagacg gtagcgaaca acgattcacc caaccttatg cttcattggc
     1081 gattcttaaa cgtgaaggtc tgacagatgt tgatgtcagc gtgggtgaat tgcgcgatga
     1141 agacggattt acaccggacg tccttcaggc gcaaatactt catggttttt cccacgggat
     1201 cactttatat ggaggtatgc aggctgctga aaattatggt tctgcagctc tgggtgtcgg
     1261 taaagatctt ggcgctttgg gcgcaatttc tttcgatgtg acacatgctc gtgcgaattt
     1321 tagccatgat gatacagaaa cgggtcagtc atatcgcttt ctctattcaa aactatttga
     1381 cgacacagac actagcttgc gcctggttgg ctatcgttac tccaccgagg gctactatac
     1441 cctcaatgag tgggcatcgc ggcgcaacag ccctgaagac ttttgggaaa caggtaaccg
     1501 acgtagtcgc gtggagggaa cgctaacgca gtcgttgggg agagattatg gcaatttata
     1561 cctgacatta agccggcaac aatactggca taccgatgat gtcgaacgat taatgcaatt
     1621 tggctacagc agtagctgga agcgtctctc gtggaacgtc tcctggagtt attccaatac
     1681 tgccagacag gggacgggga acaaccatgc cagtgataac accagtgagc agatctacat
     1741 gctctcttta tctgttcctt tatcgggctg gtggggtaat agttacgcca cctattctgt
     1801 ttcgcaaaac gataattccg gtagctcaca tcaactcgga ctcagcggta cggcgctgga
     1861 aagaaataac ctttcatgga atttaatgca gtcctataac agtcatgatg atgaggttgg
     1921 cggtaatatg tccctgacct atgatggctc ttatggcacg gtgaacggca gctataacta
     1981 cagccaaaat tcccagaggc tgaattatgg tatcagaggg ggaattctgg cacacagcga
     2041 aggggtaacg ttaagtcagg agttaggtga aactattgct cttgttaaag cacctggggc
     2101 cgccaggtta gaaatagata atatgcgcgg tgctgcgacg gactggcgcg gctatacggt
     2161 caagacacag ctaaaccctt atgatgaaaa tcgggtagca atcagcgata actatttctc
     2221 gaagtcgaat atagaacttg ataataccgt cgttacgatg gttcccacgc gtggtgcagt
     2281 ggttaaagcg gagtttgtga ctcatgtggg ttatcgcgtt ctcttcagag tgttaaatgc
     2341 aaatggtaaa ccggtacctt ttggagccat tgctgcgata caagatgcaa gtttggcaga
     2401 ttcaggaatt gtcggtgacc gtggcgaact ttatctttct ggtctaccag aaaaaggaca
     2461 ggttacgtta tcctggggag aaaacgcctc aacaaaatgc atcttcaatt attcattttc
     2521 gacaccagaa agtgagagcg gattaattga acagggtgtg acatgtcatt aaaaggattt
     2581 ttatgaacaa gtatatcaaa cagtggtgct ttgctgtgtt tatgctctcg ttaagtagtg
     2641 tagcacttgc agctcctaaa ggtatctgta cccctgataa tggcgtattt cacagtacgc
     2701 ttgatttctc gg
//
LOCUS       DAZSIO010000298         2692 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZSIO010000298 DAZSIO010000000
VERSION     DAZSIO010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.303"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..893
                     /locus_tag="ACHX5T_004124"
     CDS             <1..893
                     /locus_tag="ACHX5T_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFR8469072.1"
                     /translation="ELARVGVNPHDVAQMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQ
                     DKMQGRQVTMRGQDLDSQTAARNQAITMRGQDIQANLGQQRINLDAETNRINNENKRL
                     DRMLSAETNDLKRQEIQSRIAANNQQLQQKQQALNDGYKDGINTLTTSMFTLNDIVSS
                     PSLKSITGLRGVIPNVPGSQAADTQARLDTFKSQAYLTAVQAMRGMGALSDAEGKKLD
                     QAVGSLQNSQSEESFRRNAGVILNTLNQKRNEAVGKYVQQNGIKRVEAPQASIDYLKQ
                     HPELSIDFINRYGYLPSLGQ"
     gene            894..2624
                     /locus_tag="ACHX5T_004125"
     CDS             894..2624
                     /locus_tag="ACHX5T_004125"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR8469073.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEKRVEVA"
BASE COUNT          757 a          661 c          707 g          567 t
ORIGIN      
        1 gtgagttagc tcgtgttggc gttaatcctc atgacgtcgc tcagatgtac caacagaacc
       61 cgcggcagtt cggcgaattt gtcgatcacc tggggatgaa cagtctcggg cccgaaaaat
      121 actttgacct acaggataaa atgcagggtc gccaggttac catgcgcggt caggatctgg
      181 attcgcaaac cgccgctcgg aatcaggcaa tcacaatgcg cggacaagat atccaggcga
      241 atttaggtca gcagcgcatt aatctggacg cagaaacaaa ccgcattaac aacgaaaata
      301 agcgccttga ccggatgcta tcagcagaaa ctaacgacct gaagcgccag gaaatacaga
      361 gccgcatagc agccaacaac cagcagttgc agcagaagca gcaagcgcta aatgatggct
      421 acaaagacgg catcaacacc ctcacaacca gcatgttcac tctgaacgat atcgttagtt
      481 ctccttcact taagagcatt acaggcttac gtggagtaat ccccaacgtt ccaggctcac
      541 aggctgcaga cactcaggca cgacttgata cctttaaatc acaagcatac ctgacagcgg
      601 ttcaggccat gcgaggcatg ggcgcacttt ctgatgccga gggcaaaaag ctcgaccagg
      661 ctgttggttc gctgcagaac tcgcagagcg aggagtcctt tcgtcgcaac gctggcgtca
      721 tcctgaacac gctcaaccag aagcgtaatg aggcggttgg taagtacgtt cagcaaaacg
      781 gtatcaagcg agtggaagcg cctcaggctt ctatagatta cctgaagcag caccccgagc
      841 tgtcaatcga cttcattaat cgctacggat atcttccatc tttggggcag taaatggcta
      901 attaccgtga tttgttagag caggctggcg cacgttacgg tgtgccagaa gggttgatga
      961 ctgcactggg tgccaaggag tcttcttaca accctgccgc agtaagctcc gccggggctg
     1021 taggattgac tcaggtcatg cctgggacat ggcgtgatat gggttatacc gatgagcaaa
     1081 tgcaaaaccc cgaatatcag gctgacgctg gcgcgcgcta tctggcaaag atgtaccagc
     1141 agtttggtaa ctggcgtgac tctcttcagg cttatcacga cggtcccggc aacgttatga
     1201 aggcaaagcg tggtgaatat acgccaggac ctgaaggccg cggttacgtt gatgatcgct
     1261 ttgctcaatg ggctggtgac ccggtgacag actcaacagt cgaacagcgc gccacctctg
     1321 caaaggtaca tcctcagcaa gaccctaaca acccgtttgc acaactggaa gtacagtcat
     1381 ccgaacaagt atcggcatca ggtgtgcagt cagacccaaa taatccattt gctcagattg
     1441 agcagcaggc agccagtcag cagccacctc aacccgtaag ttctgtcgca ccgaaacctg
     1501 ttcagcaaca aacagttaat caggccaata atgcaccaac acgggaagaa ccatcattga
     1561 tgcaacaagc tggcgattgg ctcactggtg gtcaaagtgc agggcaaatt gcagaacagg
     1621 ctggtcgtgg tctggtaaac ataccatttg acgtattgca gggtggcgca agtctgatta
     1681 atgcaatcag tcaggggctt ggggggccaa aagtattgga tgatgtttat cgtccagtag
     1741 acagaccgac agacccctac gcgcaagctg gagagtcaat aggcggttat cttgttcctg
     1801 gagcaggtgt agctggaaac atggtcattg gttctctcgc tgacgcggcg aatcaacggg
     1861 gtgattttgc cgaaaatgcc gctattaatg ccggacttag cattgctacg catggcctga
     1921 taaatggcgt tacccgtggt gttcgtggtg catcaaatat aattagtggc aataaaacat
     1981 ctgcacagag agcgaccact gcgccaacag aaacatcacc attctccggt gatgccgctg
     2041 cagcaacaaa tcctgcggtt catgccgcag aggcaagagt agcacaaggt gtaccaatga
     2101 cgcctgcgac gaagaaccca gaggaagtcg ttcgcacagt agcagcacaa aaaaggccaa
     2161 atctcgcttc atcgcttgat gaactagata tcaatcctca ggctgaagtt ctggagtctg
     2221 ctgaaaggct taatgttgat tcattactcc cttcacactt ttccgggaac gagcaataca
     2281 aggcagttga gcaagcaatc aagtcccgtg cgggttctgc tctacaggtg caggaaaatg
     2341 aagcaatcag gcagctagca cagggcgcgg gtgagataat tgatcgcgtt tccggtgcaa
     2401 aagatgctct tggtatgagc gacaagttta ttgatacggt caatggaaga atgtctgcgc
     2461 tgatgaaacg aagcgaccag ctttatcgca atgttgaaaa ggcgatgcct gcaggtgcaa
     2521 aaattgatgc gccatcaaca aggtcaatgc tcaaacaggt ggcagaagat cttggcggga
     2581 tgaaaaacct tgaccctatt gaaaagagag ttgaggtagc ctgagtttaa cggacactcc
     2641 ttcctgaaat agaatggcat cagaaggagc taataatgag cagaaaaacc ca
//
LOCUS       DAZSIO010000299         2691 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZSIO010000299 DAZSIO010000000
VERSION     DAZSIO010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.304"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            144..641
                     /locus_tag="ACHX5T_004126"
                     /pseudo
     CDS             144..641
                     /locus_tag="ACHX5T_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            682..873
                     /locus_tag="ACHX5T_004127"
                     /pseudo
     CDS             682..873
                     /locus_tag="ACHX5T_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(967..1173)
                     /locus_tag="ACHX5T_004128"
                     /pseudo
     CDS             complement(967..1173)
                     /locus_tag="ACHX5T_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005160866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(1226..1477)
                     /locus_tag="ACHX5T_004129"
     CDS             complement(1226..1477)
                     /locus_tag="ACHX5T_004129"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469074.1"
                     /translation="MEPVYVILNALLDSGRFTRKLILLGLSGTFSYIFGSIVATLGMG
                     LVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLEFEKE"
     gene            1929..2213
                     /locus_tag="ACHX5T_004130"
     CDS             1929..2213
                     /locus_tag="ACHX5T_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFR8469075.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            2213..2488
                     /locus_tag="ACHX5T_004131"
     CDS             2213..2488
                     /locus_tag="ACHX5T_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR8469076.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          728 a          672 c          680 g          611 t
ORIGIN      
        1 cttattatgc ttccatcgga aacgatgatc tggcagcctg agttcacaga taaaacactc
       61 tccaggaaac ccggggcggt tcaaagcgcc agccccgtcc cgcccgatag agcgcggcat
      121 agcgggcccc ggattacttg cgtgcgacgg cagaagaagc cctgaagcga atcagtgaac
      181 tgtacgccat cgaggatgaa atacggggat taccagagtc agagtgtctt gcagtcaggc
      241 agcagcgaag caaagcgtta ctgacgtcgc tgcatgaatg gatggtggag aagaatggca
      301 cgctgtcgaa aaaatccaga ctgggcgaag cattcagcta tgtactgaat cagtgggacg
      361 ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca gcggaaagag
      421 cgcttcgtgc agtctgtctc ggaaagaaaa attacgtgtt cttcggtagc gatcacggcg
      481 gcgagcgtgg tgcactgctg tacgggctga tcggcacctg ccgtctgaac ggtatcgatc
      541 cggaagcgta tctgcgccat attctgagcg tactgccgga atggccttcc aaccgagttg
      601 acgacctcct gccatggaag gtggtgctac catcaggata atcgtcaata cggtgttcgg
      661 tgaacgttta cttggagcat catgcttcat gaagaaaaac tggcacgtca tcaacgtaaa
      721 caggcgatgt atacccgaat ggcagccttc ccggcggtga aaatgttcga agagtatgac
      781 ttcacgttcg ccaccggagc accgcagaag cagctccagt cgttacgctc actcagcttc
      841 atagagcgca atgaaaacat agtcttactg gggacatctg acatcacaaa tccccgggtg
      901 gggatttgtg tataagagac agcccatgga ggaaggagat atatcgcagc tctgacgact
      961 cactccttat ttacccggga aatacttctc cagcaatgca tacaggatgg cagcggtttc
     1021 agcatccatt atgccgtcat aatttttctg ccggaaatga agctgaaaag cgcggacaag
     1081 cgccttatat ccgtcatccg tctccacacc agaaatactg tatccatagg tcttcagccg
     1141 tgacacgata tcaaccttat ccggaatgcc ctgcgattca aaaaataaat atttagtcag
     1201 taattttatg tttttcctga aaaaattact ctttttcgaa ctccagtgag cgttcacgac
     1261 tcatcagtgt gaaaatgatg gcaaaaacag cagacagtat cagtacgata aatgtggccc
     1321 cccatcccag ataatcgaca acaagtccca ttcccagtgt cgcaacaatt gagccgaaga
     1381 tataggaaaa tgtgcctgac agccctaaca gaataagttt acgcgtaaat cgccctgaat
     1441 ccagaagagc attaagtatc acataaacag gttccatacc agtcctgacc gcatcagaac
     1501 gccagtaaat aaaatctcgt agtccaccgg catcagagac aagagtaatc gtcgttaccg
     1561 gtatttcgac tggtttgtac tggtgtgagc ggacaaaact actgtacgtc tctcatacac
     1621 gctcgtaagc ggccctcacg gcccgctaac gcggagatac gccccgactg cgggtaaacc
     1681 cttgtcggga ccactccgac cgcgcacaga ggcattttca tcgctgacag gccgcatggc
     1741 ctcgcagagc cggaggggga ctggtgatat tcggacgacc gtaccggctt gcgccgggct
     1801 tcggcggttt tacacctgtg ctacatgtaa ccaccgtatg ccgccttata tgcgctgact
     1861 cgcattattt gccttgttcc gtctgaaagt aatcactatg attaaatatg attaacaact
     1921 aatcgggtat gcaaatgaaa aacaataccg cacaagcaac aaaagtaatt accgcgcatg
     1981 tgccattacc tatggctgat aaagtcgacc agatggccgc cagactggaa cgctcccggg
     2041 gctgggttat caaacaggcg ctttctgcat ggcttgccca agaggaggag cgtaatcgcc
     2101 tgacgctgga agccctggac gatgtgacat ccggacaggt tatcgaccat caggctgtac
     2161 agtcctgggc ggacagcctc agtactgacc atctgttacc ggtgccacgc tgatggaact
     2221 gaagtggacc agtaaggcac tttctgattt ggcgcggtta tatgattttc tggtgctggc
     2281 cagtaaacct gctgccgcca gaacggtgca gtccctgaca caggcgccgg tcattctgtt
     2341 aactcatcca cgtatgggag aacagttgtt tcagtttgaa cccagggagg tcagacggat
     2401 ttttactggc gagtacgaaa tccgttacga acttactggc cagactattt atgtattgcg
     2461 cctgtggcac acacgagaga acaggtagat tgggtgtgga ggcaacttta acatagcctg
     2521 caggtattaa ttctggagca ttgttatcag gccagatatt ttgcattcca ttaaataatc
     2581 ttgtaggaga taaaataaat tacgatgaaa taacgaaaat taccgctcgt gactggcggc
     2641 aatatcgcgc tccgggctgg caaataaccc atcagaaacg ttattgccag a
//
LOCUS       DAZSIO010000300         2686 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZSIO010000300 DAZSIO010000000
VERSION     DAZSIO010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.305"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(307..1086)
                     /gene="ipaJ"
                     /locus_tag="ACHX5T_004132"
     CDS             complement(307..1086)
                     /gene="ipaJ"
                     /locus_tag="ACHX5T_004132"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HFR8469077.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(1283..2092)
                     /locus_tag="ACHX5T_004133"
                     /pseudo
     CDS             complement(1283..2092)
                     /locus_tag="ACHX5T_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2439..>2686
                     /locus_tag="ACHX5T_004134"
     CDS             2439..>2686
                     /locus_tag="ACHX5T_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8469078.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
BASE COUNT          803 a          676 c          434 g          773 t
ORIGIN      
        1 taaaatacat ctattttcaa ataaaataaa gcatactcca attacaacga gtgactaaaa
       61 taaaaatatc atccaagtca atcactgttt gctctgcaaa atatgtcagc cttctcaaac
      121 ttaccagcac ttaaatattt aagcacacaa tcattccttg agggagttca cttatttcaa
      181 gagaaacatt tcgtcttccc acacaactaa tccgcccaat atagctaaat ttgtactcag
      241 taatatcatt tgttatgccc cccaccccaa aatattcaag ctccttcaca gataaaaaac
      301 gacaatttac aaagcctcat tagttataac tatggaaata cctattttag tatatcctat
      361 aaagttagaa ttacttcttc ttgcacctaa ttccatagtt gaaaaacaag aataattttc
      421 tcctacagct ggatccatat agcttccatc aggtctacac aacacccagt ggaggccgac
      481 aggaactcca ataaaggata cagcaacagc ttccaaaact ctttggttag aagataacac
      541 atctcgttga tgtactatat tacatcccat gccaattaat aagtcccttg catcgggata
      601 aataaaacta attacttgcg aaaaaatgtt actttcttca acgacataag cattcaatcc
      661 aagaagcctc gtagccatga ctatcttatc aggcatagaa taaccggcat ccgcgatatt
      721 atctttgtgt attctcggat tataattacc actagttatt aagtataaat cccgctcaca
      781 acgattatcc aaatcaagag aactcttcct tgtcatttcg gacatagacc ctttgtattg
      841 tggtatttta tcaaccccta attcctttgc agcacagagt aaactggcag ctccacaaga
      901 attgtcatac atctgcttcc tcgccaaaaa acaaccttgt tcgtttactc tattctcatt
      961 taccctcaca tcagtctgat gagatatcat atattctctt ggtatagccc cctgcaataa
     1021 aaccccatat aacataacac ctgtatgaat acatccccgt ttgcaaggct tccgttgttc
     1081 cgacatcgac gagtaattgc agcacagtat tgcatccagc aatgcgaact actatgagaa
     1141 ttagctgacc gggtccttat tccccgaacg aactttaaat ccaaaacctc atactctttt
     1201 ccgtcgtcat cacgtccgtc accataacaa cctttcagca tccacccttt tattaagagt
     1261 taatgtccat cagcatcagc aattaatacc gcctgatact gacatatttt tgtcgtattt
     1321 acgtccataa tcggttattg tgatacaggt cacaccagct tatttgctat tgttacaata
     1381 accaccttgg ttgagagctg ttttttcaac tttgttatca actcccccaa ctccttggtc
     1441 agtaaggctg ccgaccgagc acagtgaata gatagtatcc gcaggtacca ccatgcttac
     1501 ttaatactca gaaggcgaag tgctctccaa ttaactctgc cgcactatgc ttataagctg
     1561 cttttcaatg ccgtctatca acatgtccaa ttcattcaat ctgttgtact ggtaatccaa
     1621 cgcagtaatg atatatgcag gtattctttc atccactcca gtaacgtttc atgcaaagca
     1681 ttaatttacg cgctacggaa cttcaccagt tgactgagca aacgatgtgg gaccagtact
     1741 gcctgcagtt cttctattgt tacagcgatt tctttaccaa acagctgaac caccatcaag
     1801 ataaagatag cctgaacatt catcgcacca ttttgttacc aatggcaaaa gatttaacga
     1861 accgtgcctg catcaggcgg actttttatc cctgctttgt tagctcccta accttgtaca
     1921 gagagcctcc ataagctccc atatcaagca gacatggttc gcagttactg acatacacca
     1981 gaaaactctg acagcagact cacttatcct ccacttcact catatcctta ttggtgagat
     2041 gaacctgaat gacatgtttt gcgacataaa cgccaaccag tgtatatttc acctgtagag
     2101 attctacaat ctgtagcgtt atgcatctaa tgttaggctc tatgatgtca gaaatctgcg
     2161 agactctaca ccttctgcat ttacctttat ttatcaccat gctcctcagt taacggagaa
     2221 cgtgttcatt gcattttgtc acttacattt cgcctttggt atcgggggga tataggttcc
     2281 aacgttccgc atgagaacct gaaccggctt cacgccacct tgataccaga tgccgcacag
     2341 gtagtcaaca agtgtcacga acggtgcaat agtgatccac acccaacgcc tgaaatcaga
     2401 tccagggggt aatctgctct cctgattcag gagagcttat ggtcactttt gagacagtta
     2461 tggaaattaa aatcctgcac aagcagggaa tgagtagccg gacgattgcc agagaactgg
     2521 ggctctcccg caataccgtt aaacgttatt tgcaggcaaa atctgagccg ccaaaatata
     2581 cgccgcgacc tgctgttgct tcactcctgg atgaataccg ggattatatt cgtcaacgca
     2641 tcgccgatgc tcatccttac aaaatcccgg caacggtaat cgctcg
//
LOCUS       DAZSIO010000301         2605 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZSIO010000301 DAZSIO010000000
VERSION     DAZSIO010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.306"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            127..462
                     /gene="eutS"
                     /locus_tag="ACHX5T_004135"
     CDS             127..462
                     /gene="eutS"
                     /locus_tag="ACHX5T_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR8469079.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            475..954
                     /gene="eutP"
                     /locus_tag="ACHX5T_004136"
     CDS             475..954
                     /gene="eutP"
                     /locus_tag="ACHX5T_004136"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR8469080.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            929..2302
                     /gene="eutA"
                     /locus_tag="ACHX5T_004137"
     CDS             929..2302
                     /gene="eutA"
                     /locus_tag="ACHX5T_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR8469081.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(2373..>2605)
                     /locus_tag="ACHX5T_004138"
     CDS             complement(2373..>2605)
                     /locus_tag="ACHX5T_004138"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469082.1"
                     /translation="CAVRLTNLRTTRRTCEDLSILSFLVFIFHFLFSSLSGSVLVLVF
                     SFLFLFFLQGYAKTPKHFSPQKHPIILPDQSP"
BASE COUNT          626 a          650 c          730 g          599 t
ORIGIN      
        1 ataacagtaa atatctctcc attcttattg atctacctca ccttttaaac gcgcttgccg
       61 aattttgtta tttactctga cgaaaaattg tcacgataca cgaaagtttt tcacaggcgg
      121 cgactcatgg ataaagaacg catcattcag gaatttgtgc ccggcaaaca ggtcacactg
      181 gcgcatctca tcgcgcatcc cggtgaggaa ctggcgaaga aaatcggcgt tccggatgcg
      241 ggcgcaattg gcattatgac gctaactccc ggcgaaacgg cgatgatcgc cggtgattta
      301 gcgctgaaag ccgctgatgt gcatatcggt tttctcgacc gcttcagcgg tgcattggtg
      361 atttatggct cggtgggcgc agtagaagaa gcgttatcac aaaccgtcag cggtttaggt
      421 cgcttattaa attatacgct ttgtgaaatg accaaaagtt aatacgaggc attcatgaaa
      481 cgaattgcgt ttgtgggtag cgtcggggca ggaaaaacaa cgttatttaa tgcgttgcag
      541 gggaattata ccctcgccag aaaaacactg gccgtggaat ttaatgataa gggcgatatt
      601 gatacgccgg gtgaatattt tagccatccc cgctggtatc acgccttaat taccacgcta
      661 caggatgtag acatgctgat ctatgtccat ggcgcaaatg acccggaaag tcggttgcct
      721 gccgggttgc tggatattgg cgtcagcaaa cggcaaatcg ccgtcatcag taaaacggac
      781 atgccagatg ctgacgtcgc cgcaacgcga aagttattgc ttgagacggg tttcgaagag
      841 ccgattttcg agctaaacag ccacgacccg caaagcgtgc agcaactggt ggattatctg
      901 gcatctctca ccaaacagga ggaagcaggt gaaaaaactc atcacagcga atgatattcg
      961 tgaagcacac gcgcgcggcg aacaggcaat gtccgtggtt ctgcgcgcca gcattattac
     1021 cccggaagcc cgtgaagtgg cggatctact gggttttacc atcaccgaat gtgatgaatc
     1081 gatcccggta acggcgtccg tgcccgccag cgtgccagcc gacaaaaccg aaagccagcg
     1141 catccgcgaa accatcattg cccagctgcc ggaaggccag tttaccgaaa gcctggtcgc
     1201 acagctgatg gaaaaagtga tgaaggaaaa acagtcgctg ggcgattttg tcgtcgccag
     1261 cgccgggccg cacctcgaat ccgtgatcgc cggtcacgga gccggggcgc aaaccctttc
     1321 tgaacaacgg ctgtgtcggg tactgaatat cgatatcggc ggtggcaccg cgaactacgc
     1381 cctgttcgat gccggaaaaa tcagcggcac tgcctgtctc aacgtcggtg gtcgcctgct
     1441 ggaaaccgac agccaggggc gcgtggttta cgctcataaa ccggggcaga tgattgtgga
     1501 tgagtgcttc ggtgcaggca ctgacgcccg ttcgctgacc ggcgcgcagc tggtgcaggt
     1561 tacccggcgg atggcggcgc tgattgtcga agtgattgac ggaacgcttt cgccgctcgc
     1621 gcaggcattg atgcaaaccg gtttgctgcc cgcaggtgtt acgcccgaaa tcattacgct
     1681 ttctggaggc gtgggcgaat gttatcgcca ccagcccgcc aacccgttct gttttgccga
     1741 tattggccca cttctggcaa cggcgctgca tgaccatccg cgcctgcgtg agatgaatgt
     1801 gcagtttccg gcgcaaaccg tacgctccac ggtgattggc gcgggtgcac ataccctttc
     1861 gctctctggc agcacaatct ggctggaggg cgtacaactg ccgctgcgca atttgccggt
     1921 ggcgatcccg attgatgaaa cggatctggt gagtgcctgg caacaggcgc tgcttcagct
     1981 ggatcttgat cccaaaactg acgtgtacgt gctggcgctt cccgcctcgc tgcctgtgcg
     2041 ttacgccgcg gttctgacgg tcatcaacgc gctggtcgat ttcgtcgcgc gttttccgaa
     2101 tccgcatccc ctgctggtgg tggccgggca ggactttggt aaagctctgg gcatgttgtt
     2161 gcgcccacag ctacaacaac tcccgttggc agtcattgac gaagtgattg tccgcgcggg
     2221 ggactatatc gacattggta cgcctctttt tggcggatcg gttgtgccgg tgacggtgaa
     2281 atcactcgca tttccttcct gaatacggta gttatcaggt atggtgcttg gtgtctgggt
     2341 gttagtggga ttatatccgg cagtgtatcc ggttaaggtg attggtctgg caagatgatt
     2401 ggatgctttt gtggtgaaaa atgttttggt gttttagcat aaccctgaag aaagaataaa
     2461 aataaaaagg aaaaaaccaa aaccagaaca gaaccggaaa gagaagaaaa gagaaaatga
     2521 aaaataaaaa ccaaaaaaga aagaatactt aaatcttcgc atgttcttcg ggtggtacgc
     2581 aagtttgtta gtctgaccgc acaat
//
LOCUS       DAZSIO010000302         2592 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZSIO010000302 DAZSIO010000000
VERSION     DAZSIO010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.307"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            399..542
                     /locus_tag="ACHX5T_004139"
     CDS             399..542
                     /locus_tag="ACHX5T_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR8469083.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            555..764
                     /gene="cspE"
                     /locus_tag="ACHX5T_004140"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             555..764
                     /gene="cspE"
                     /locus_tag="ACHX5T_004140"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR8469084.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            930..1739
                     /gene="rlmA"
                     /locus_tag="ACHX5T_004141"
     CDS             930..1739
                     /gene="rlmA"
                     /locus_tag="ACHX5T_004141"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR8469085.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1736..2302)
                     /gene="mntP"
                     /locus_tag="ACHX5T_004142"
     CDS             complement(1736..2302)
                     /gene="mntP"
                     /locus_tag="ACHX5T_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR8469086.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     regulatory      complement(2434..2524)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
BASE COUNT          689 a          610 c          624 g          669 t
ORIGIN      
        1 tattttaaat ttacaatccc tttggggatt gacttctctt tagggtaatt gatagccgtt
       61 aactgactgt tttatgagaa aaagtgatat aactttttat tcattgcata gcagaaaatg
      121 tgatattgca cgcactatgt aataacttct ccctctggcc tggaacaact gaacttattg
      181 aactatgtta gaaactacgc cagtttaagt atctgcctga actggcaagg ttaagcacaa
      241 tgatatatcg gcgcgtattc cgttgcataa gtgtgcaaaa aaagtggaag acgtatcgag
      301 atttgtgcgt ctgatcgaga tatgtttaaa aatggcttgc cataattaac gttgtatgtg
      361 ataaaagatt tcgggttaaa cgaggtacag ttctgtttat gtgtggcatt ttcagtaaag
      421 aagtcctgag taaacacgtt gacgttgaat accgcttctc tgccgagcct tatattggtg
      481 cctcatgcag taatgtgtca gttttatcta tgttatgcct gcgggcgaag aaaacaatct
      541 aaggaatttt tcaaatggca aagattaaag gtcaggttaa gtggttcaac gagtctaaag
      601 gttttggctt cattactccg gctgatggca gcaaagatgt gttcgtacac ttctccgcta
      661 tccagggtaa tggcttcaaa actctggctg aaggtcagaa cgttgagttc gaaattcagg
      721 acggccagaa aggtccggca gctgttaacg taacagctat ctgatcgaat ccactgatct
      781 gaagtgtgaa tacgcttcaa tctcgctata aagcctcgtc gaatgcgagg ctttttacta
      841 tgctttatct tcgctcctgg cgttcggata tttgcccgcc gcgtgattcg cgttacactt
      901 gcggccttta gtatcctgcc ggagttgtca tgtcgttttc ctgtccactt tgccatcagc
      961 ctctttcgcg tgaaaaaaac agctatatct gtccccaacg acatcagttt gatatggcga
     1021 aagaagggta tgtcaatctg ctgcccgttc agcataaacg gtctcgtgat ccgggcgaca
     1081 gcgcggaaat gatgcaagca cgccgcgcat tcttagatgc cggacattat cagccgctgc
     1141 gtgatgcaat tgtcgcccaa ctgcgggaac ggcttgatga aaaggccacg gcggtgctgg
     1201 atattggctg tggtgaaggg tattacacac acgcatttgc cgatgcgttg cccgaaatca
     1261 ccacgtttgg cctggatgtc tcgaaggtag cgataaaagc ggcggcgaaa cgctatccgc
     1321 aggtcactct ttgtgtcgct tccagccacc gtttgccgtt ttccgatacc agtatggacg
     1381 ccataatacg tatttacgcg ccgtgtaaag cagaagaatt agcacgagta gtgaagcccg
     1441 gcggctgggt cattactgcc acgccgggac cgcgacattt gatggagctg aaggggctga
     1501 tttacaatga agtacatctt catgcacctc atgcagaaca actggaaggt tttacattac
     1561 agcagagtgc ggagttgtgt tatccgatgc gtcttcgcgg tgatgaagcc gtcgcattat
     1621 tgcagatgac gccgtttgcc tggcgtgcga agccagaagt ctggcaaaca ctggcagcaa
     1681 aagaagtgtt cgactgccag acggacttta atattcacct ctggcagcgt tcttattaac
     1741 cgtggaagtg cgtccagagg atctggacgc cgatgccgat cagcaccagc ccgccgagaa
     1801 tttccgcttt tttcccaata attgagccga taaagcgacc aaccatcatc cctaatgtcg
     1861 acataatcaa ggttgcacaa ccaatggcca atgcggtcgc gataatgttg acctgaagga
     1921 aagcaagacc aacacccaca gccatggcat ccaggctggt ggcaatcgcg gtggttacca
     1981 gtagccagaa accgtgccga cggcgcggct cttcatcttc atcatctgcg ccacgaaaac
     2041 cctcaataat cattcgcccg ccgaggaata tcagcagcac aaacgcaatc cagtgattcc
     2101 attcaaggac aaaccggctg gctaacatgc ccattcccca gccgatcagc ggcgtcaggg
     2161 tttcgacggc accaaaaata aggccggttc gcaatgcttc agaaaatttc ggtttatgga
     2221 ggggggcacc tttaccgatt gatgcagcaa atgcatccat cgacatacca aacgcaagaa
     2281 gaacagtagc agtgatattc atgacaacgt cctgaccggg gtatccataa aacacatcat
     2341 cgcccccagc aaacatgcat acatgaacaa caagccgcga tgggttttcg cgttcatgca
     2401 ggcatagcga ttgatgtgca tatggtctcg cctgactgaa agtcttcagt ccgtacgtgc
     2461 cacgttttgc aacgagtatg ttgacacgta catttccgga atctggaaat cggctactcc
     2521 ccaaatgagg gcgcaacctt aacatatttt gagaatataa aacaactgtt ggaatatatt
     2581 atttcattgc tt
//
LOCUS       DAZSIO010000303         2573 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZSIO010000303 DAZSIO010000000
VERSION     DAZSIO010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.308"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(64..822)
                     /locus_tag="ACHX5T_004143"
     CDS             complement(64..822)
                     /locus_tag="ACHX5T_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR8469087.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="ACHX5T_004144"
     CDS             complement(830..1933)
                     /locus_tag="ACHX5T_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR8469088.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..>2573)
                     /locus_tag="ACHX5T_004145"
     CDS             complement(1943..>2573)
                     /locus_tag="ACHX5T_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFR8469089.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          711 a          684 c          601 g          577 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat aacggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aac
//
LOCUS       DAZSIO010000304         2557 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZSIO010000304 DAZSIO010000000
VERSION     DAZSIO010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.309"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            56..1486
                     /locus_tag="ACHX5T_004146"
     CDS             56..1486
                     /locus_tag="ACHX5T_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR8469090.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLAYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(1527..2459)
                     /locus_tag="ACHX5T_004147"
     CDS             complement(1527..2459)
                     /locus_tag="ACHX5T_004147"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR8469091.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
BASE COUNT          800 a          528 c          565 g          664 t
ORIGIN      
        1 tatttcagca taagtggata ttcaaagttt tgctgtttta tcagggaata tttatatgat
       61 acgtaagtca gctacaggtg ttattgttgc gttagccgta atctggggtg gtggcacatg
      121 gtacacaggt acgcaaattc agcctggtat cgaaaagttt attaaagatt ttaacgatgc
      181 gaaaaagaaa ggtgaacatg cctacgatat gacgttaagt tataaaaact ttgacaaagg
      241 cttttttaat tcccgttttc aaatgcaaat gacattcgat aacggtgcac ccgatctcaa
      301 tatcaagcca ggccagaaag ttgtatttga tgtggatgtt gagcacggtc cgttgcccat
      361 cacaatgtta atgcatggta atgtcatccc agcactggca gcggcaaaag tgaacttagt
      421 gaataatgaa ctgacacaac cgctatttat cgccgcgaaa aataaatcgc ccgtggaagc
      481 gacattgcga ttcgcgtttg gtggctcatt ctctacgaca ttagatgttg cccctgcaga
      541 gtatggaaaa ttttcttttg gtgagggcca gtttactttt aatggtgatg gtagttcatt
      601 gtctaacctg gatattgaag gcaaagtcga agatattgtt ctgcaattat caccaatgaa
      661 caaagtaacg gcaaaaagtt ttaccattga ttctctggcg cgattagaag aaaagaaatt
      721 tccggttggt gaaagcgagt cgaaatttaa tcagattaac attatcaatc acggggaaga
      781 cgttgcccaa atcgatgctt tcgttgcaaa aaccaggctg gatcgcgtta aagacaaaga
      841 ttatatcaat gtcaatctgg cctacgaact tgataagtta acaaaaggga atcagcaact
      901 cggtagtggt gagtggtcat tgattgctga atctattgat ccctcagcag tgcgccaatt
      961 tatcatccag tataacattg cgatgcagaa gcagcttgct gcacaccctg agttagcaaa
     1021 cgatgaagtt gctctgcaag aagtgaatgc tgcattgttc aaagagtatt taccgttatt
     1081 acaaaaaagt gagccgacca ttaaacaacc ggtaagatgg aagaacgcac tcggcgaact
     1141 aaatgccaat ctggatatca gtattgccga cccagccaaa tcttcatcat ccacaaacaa
     1201 agatatcaaa tcgctcaatt ttgatgtgaa gttaccgctt aatgtcgtca cagaaaccgc
     1261 aaaacagctt aatttatctg aaggaatgga tgcggaaaaa gcgcaaaagc aggctgataa
     1321 acaaatcagc gggatgatga cattaggtca gatgtttcag ttaatcacga ttgacaacaa
     1381 taccgcctcg ctgcagctgc gttatacacc gggtaaagtt gtttttaacg gacaggagat
     1441 gagcgaagaa gaatttatgt ctcgtgccgg acgttttgtt cattaaaata ctcactcgcc
     1501 tctgctcatt cagaggcgag taaaacttac ttattctgac gctgtgatga catgagagac
     1561 gtcagaagcc ggtctttttc ctgccacaat gagttcagcc attgctgaaa atggcgctta
     1621 aagcttttgt cattgatgta gtcgccatgt aactcatcgg caataggctg taaatccaca
     1681 tgtacgacaa tccgcgtcag tttaccgctt aacatatcga agaacggctg acggttattg
     1741 tccggataac acagcgtaac attaagcagt ttatcgaatt gtttacccag tacatttagc
     1801 gccatcgcaa tgcctgccgc ctttggtggc aacaagtttt gaaaagtgga gtgggtttgc
     1861 tgatgttttt cttgcgtgaa acgggagcct tcaacgaaat tgacaatggt ggtgggatgc
     1921 aggcgaaact tttcacaaga acgccgagtg gtttcaacat cttgaccacg tcgctccgga
     1981 tgacgtaata aataagcgcg ggaataacgc ttcataaacg gcatatccag cgcccagcac
     2041 gccaggccaa gaaatggcac ccaggccagc tgttgcttga ggaaatattt attcatcgga
     2101 atgtgcttac gaaacagcac gcacagtacg acaatatctg cccaactacg gtggttacaa
     2161 ataagcagat accagttctt cttacttaac ccttccagcc cgtgaacttc ccattgcagg
     2221 tgtgggttca gatgcagtaa taccgccaga ccttcacacc agcaatacat cataaaatca
     2281 caaaaacgcg aaacctttcg ccaaatgact ggcacaggta gcaatagttt tacaatcccg
     2341 gcaatgatga tcgggacgga acaaaaaata gtgaccaaaa tggtcaatac gatactcagc
     2401 aaaagggtta tcgcagcgag tattctcgtc ataatgaatt tattcaaaag gttagccata
     2461 gacagtgcac taaagaagcg caaggcgcag attttagcag aaaatgaccc aaataacgga
     2521 tgatccttaa ggagaaaaat aattcatatc tatccac
//
LOCUS       DAZSIO010000305         2551 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZSIO010000305 DAZSIO010000000
VERSION     DAZSIO010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.310"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            208..2487
                     /gene="ydeP"
                     /locus_tag="ACHX5T_004148"
     CDS             208..2487
                     /gene="ydeP"
                     /locus_tag="ACHX5T_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR8469092.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
BASE COUNT          668 a          587 c          645 g          651 t
ORIGIN      
        1 atattggaaa atatgttaat ttaaacacta tgagagatga tgcttaactt atcgtattta
       61 atctattgtt taacgataat agattaaaaa tagaaagaga gtagtccctt actcttatat
      121 atccatgttg gcgataatcc ctttgtatgt actgtgcatc atcgctatta caaatcctaa
      181 taattcattt ccacacagga taagtagatg aagaaaaaaa ttgaatccta ccagggtgct
      241 gcaggtggtt ggggtgctgt taaatccgta gcgaatgcag tacgtaagca gatggatata
      301 cgccatgatg ttattgccat gtttgacatg aataagccag agggctttga ctgtccgggt
      361 tgtgcatggc cagatcctaa gaacagtgcg tcattcgaca tttgtgaaaa cggcgcaaaa
      421 gcaatcgctt gggaagtcac ggataagcag gtaaatgcct ctttctttgc tgagaatacg
      481 gttcaatcat tacttacctg gggagatcac gagcttgagg ctgcggggcg tctcactcag
      541 cctttgaaat atgatgccgt cagcgactgt tacaagccat taagctggca acaagctttc
      601 gacgaaattg gcgcacgcct tcaaagctat agtgatccca atcaggttga attctatact
      661 tcgggccgca cttccaatga agctgccttt ctttatcagc tttttgcccg tgaatacggg
      721 agcaataact ttcccgactg ctccaacatg tgccatgaac cgacaagcgt gggtttggca
      781 gcgagtatcg gtgtaggtaa agggaccgtg ttgctggaag actttgagaa gtgcgattta
      841 gtcatttgca ttgggcataa ccctggtaca aaccaccctc gcatgctgac ttcgttgcgc
      901 gctttagtga aacggggagc gaaaatgatc gccatcaatc ctctacagga acgtggcctg
      961 gagcgattta ccgcaccgca aaacccgttt gaaatgctga cgaactctga gactcagttg
     1021 gccagtgcct actacaacgt gcgcattggt ggcgatatgg cgttgctcaa ggggatgatg
     1081 cgcctgttaa ttgagcgcga tgatgctgca agtgccgcag gtcggccctc attgcttgat
     1141 gacgaattta ttcaaacgca taccgtcggc tttgacgagc tacgccgtga cgttctcaat
     1201 tccgagtgga aagatatcga acgtatttct ggactaagtc agacacaaat cgccgaactg
     1261 gctgacgcat atgccgctgc cgaacgcacc attatctgtt acggaatggg gatcactcag
     1321 cacgaacatg gtacccagaa cgtgcagcaa ctggtcaatt tgctgttgat gaaaggtaac
     1381 attggcaagc ctggtgcggg tatctgccca ctacgtggtc actctaatgt acagggcgac
     1441 cgaaccgtcg gtatcaccga gaaaccgtct gcagagtttc tggctcgcct gggtgagcgc
     1501 tatggcttcc ccccacctca tgcacctgga catgctgcaa ttgccagcat gcaagcaata
     1561 tgtacggggc aggctcgagc attgatctgc atggggggca actttgcact ggcaatgcca
     1621 gatcgggaag cgagcgctgt accgttaacg caattagatt tggcggtaca cgtagccact
     1681 aagcttaacc gctctcatct gctgaccgca cggcatagct atattctgcc ggttctgggg
     1741 cgtagcgaga ttgatatgca aaaaagcggt gcgcaggcgg taaccgttga ggattcaatg
     1801 tcgatgattc atgcctcgcg tggcgtgtta aaacccgccg gtgtaatgct gaaatcagag
     1861 tgtgcagtgg tcgcgggaat cgcgcaggca gcactacccc agagcgtggt agcctgggag
     1921 tatctggtgg aagattatga tcgcattcgc aatgacattg aagctgtgct gccagagttc
     1981 gccgactata accagcgcat ccgtcatccc ggtggttttc acctgataaa tgcagctgct
     2041 gaaaggcgct ggatgacgcc gtcaggtaag gctaatttca ttaccagcaa agggctgtta
     2101 gaagatccct cttcagcgtt taacagtaag ctggtcatgg cgacagtacg cagccacgat
     2161 cagtacaaca cgacgattta tggtatggat gatcgctatc gaggggtatt cggtcaacga
     2221 gatgtggtct ttatgagtgc taaacaagct aaaatttgcc gtgtaaaaaa cggcgaaaga
     2281 gttaatctta ttgcgcttac gccagacggt aagcgcagct cacgccgcat ggatagatta
     2341 aaagtggtca tttaccctat ggctgaccgc tcactggtga cctattttcc agaatcgaat
     2401 cacatgctaa cacttgataa ccacgatcca ttaagtggta ttcctggcta taaaagtatt
     2461 ccggttgaat tagaaccatc aaattaatgc ctcttctcat ttcttctgct gtcatccgca
     2521 cagcagaaga attcctcatt gactattatt g
//
LOCUS       DAZSIO010000306         2492 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZSIO010000306 DAZSIO010000000
VERSION     DAZSIO010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.311"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>2492
                     /locus_tag="ACHX5T_004149"
     CDS             <1..>2492
                     /locus_tag="ACHX5T_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR8469093.1"
                     /translation="QEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDV
                     NAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAH
                     QDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIV
                     VGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYY
                     RLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQS
                     LFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERD
                     LRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQM
                     VNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAE
                     NLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGV
                     GELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDN
                     IWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAAD
                     RQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTV
                     KEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSR
                     LSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQNVQRSL
                     DIQQRSVQQLANTIINSLIQYDDPAAWTEQEQ"
BASE COUNT          730 a          578 c          602 g          582 t
ORIGIN      
        1 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
       61 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
      121 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg
      181 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
      241 gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt
      301 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat
      361 acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg
      421 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg
      481 gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt
      541 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
      601 tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
      661 ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg gagcatgtta
      721 gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt
      781 tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt
      841 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
      901 accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc
      961 acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt
     1021 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
     1081 ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag
     1141 ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa
     1201 aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg
     1261 gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg
     1321 gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt
     1381 gccaaggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tattgccgta
     1441 tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat
     1501 aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa acgctggagt
     1561 gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt
     1621 agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg aatcacccac
     1681 agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa
     1741 acgctcgacc agcgtccagc ggagaaattc gcccagctga tgtatgagac gcgctatgct
     1801 gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc cgcagatgcg
     1861 ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt
     1921 aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggctc aatcaaacac
     1981 tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt
     2041 actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc
     2101 cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca
     2161 aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt ttcttctcgc
     2221 ctctcggttg atcctcaggc taaagatatc agtcatttgc tggcttttac ttgtcaacca
     2281 gaacgacatg atgaacagtt aacgttagcg aatgaagtga tggttaagcg tttgactaag
     2341 gggatcagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag cctcgatatc
     2401 caacagcgta gcgttcaaca attagcgaac actattataa atagtcttat tcaatatgac
     2461 gatcctgcag catggactga gcaggagcaa tt
//
LOCUS       DAZSIO010000307         2478 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZSIO010000307 DAZSIO010000000
VERSION     DAZSIO010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.312"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            90..2357
                     /gene="adiA"
                     /locus_tag="ACHX5T_004150"
     CDS             90..2357
                     /gene="adiA"
                     /locus_tag="ACHX5T_004150"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR8469094.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT          628 a          672 c          609 g          569 t
ORIGIN      
        1 aatgtttgtt tttcacgcgc tttacagccc gaaaaggccg gaagatactt gcccgcaacg
       61 aagattcctt cataaccggg taagcaatga tgaaagtatt aattgttgaa agcgagtttc
      121 tccatcaaga cacctgggtc ggtaacgccg ttgagcgtct ggcagatgct ttaagccagc
      181 aaaatgttac cgtgattaaa tccacctcct ttgatgatgg ttttgccatt ctctcttcaa
      241 acgaagccat tgactgcctg atgttcagct atcaaatgga gcatccggac gaacatcaaa
      301 acgtcagaca attgatcggt aagcttcatg agcgccaaca aaacgtgccg gtcttcctgt
      361 tgggcgatcg ggaaaaagcc ctcgccgcaa tggaccggga tctgctggag cttgtcgatg
      421 aattcgcctg gattctggaa gataccgccg actttatcgc cggacgcgcc gttgccgcga
      481 tgacccgcta ccgccagcag ctgttgccgc cactgttcag cgcgctgatg aaatatagtg
      541 acatccatga atattcctgg gcagcgccag gccaccaggg cggcgttggt tttaccaaaa
      601 cacccgccgg acgtttctac catgactact atggtgaaaa tctgttccgt accgacatgg
      661 gcatcgaacg aacttccctc ggttctttgc ttgaccatac tggcgcattt ggcgaaagcg
      721 aaaaatatgc cgcacgcgta tttggtgccg atcgctcctg gtcggtagtc gtcggtactt
      781 ccggctctaa ccgcaccatc atgcaggctt gcatgaccga taacgatgtc gtggtcgttg
      841 accgtaactg ccataaatcc atcgaacaag gtttgatgct gacaggcgcg aaaccggtct
      901 atatggtgcc aagccgcaac cgctacggca ttatcgggcc aatctatccg caggaaatgc
      961 aacctgaaac cttgcaaaag aaaatcagtg aaagcccgct gaccaaagac aaagccgggc
     1021 aaaaaccgtc ttactgcgtg gtaaccaact gcacctatga cggcgtgtgt tataacgcta
     1081 aagaagcgca ggatctgctg gaaaaaacct ccgatcgtct gcactttgac gaagcctggt
     1141 acggctatgc acgtttcaac ccgatctatg ccgatcacta tgccatgcgc ggcgaacctg
     1201 gcgatcacaa cggtcctacc gttttcgcca cccactccat ccacaaactg ctgaatgcgc
     1261 tgtcacaggc ttcttatatt catgtacgtg aaggtcgtgg ggcgattaac ttctcccgct
     1321 tcaaccaggc ctacatgatg catgccacca cctccccgct gtatgccatc tgcgcatcca
     1381 acgacgtggc ggtgtcgatg atggacggca acagcggcct gtcactgaca caggaagtga
     1441 ttgacgaagc ggttgatttc cgtcaggcga tggcgcggct atataaagag ttcaccgctg
     1501 acggtagctg gttcttcaaa ccgtggaaca aagaagtcgt caccgaccca caaaccggca
     1561 aaacctatga ctttgctgac gcaccaacca aactgctgac caccgttcag gactgctggg
     1621 taatgcatcc gggcgaaagc tggcacggct tcaaagatat tccggataac tggagtatgc
     1681 tcgacccaat taaagtcagc atccttgctc cgggaatggg tgaagatggt gaactggaag
     1741 aaaccggtgt tccggcggcg ctggtcactg cctggcttgg tcgccacggc attgtgccta
     1801 cccgcaccac tgacttccaa attatgttcc tgttctctat gggcgtaacc cgtgggaaat
     1861 gggggactct ggttaacacc ctttgctcct tcaaacacca ctatgacgcc aacacaccgc
     1921 tggcgcaggt gatgccggaa cttgttgaac aatatcctga cacttacgcg aacatgggga
     1981 ttcacgatct gggtgacacc atgtttgcct ggctgaaaga aaacaaccct ggcgcacggt
     2041 tgaacgaagc ctattccggc ctgccggtgg cggaaatcac cccgcgtgaa gcgtacaacg
     2101 cgattgtcga caacaatgtc gaactggtat ccattgaaaa tctgccagga cgcatcgcgg
     2161 caaactcagt tatcccgtat ccgccaggaa tcccgatgct gctgtctggt gaaaacttcg
     2221 gcgataaaaa cagtccgcaa gtaagttatt tacgctcgct gcaatcctgg gaccaccatt
     2281 tccctagatt tgaacacgaa actgaaggga ctgaaattat tgacggtatt taccacgtta
     2341 tgtgcgtgaa agcgtaacca ctattccgct gaaggcgtaa ttgtttaaat aacattacgc
     2401 cgcctggcct taggcctttt gagtatggca acgttttcat aaaaattgct gcaaacaaaa
     2461 atgtcatact ttttgcgc
//
LOCUS       DAZSIO010000308         2462 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZSIO010000308 DAZSIO010000000
VERSION     DAZSIO010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.313"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..448
                     /locus_tag="ACHX5T_004151"
     CDS             <1..448
                     /locus_tag="ACHX5T_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8469095.1"
                     /translation="ESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGT
                     KAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWE
                     SIEMVRRYAHLAPNHLTEHARQIDLILNPSVPNLSQSRNKEGTNDV"
     gene            798..1160
                     /locus_tag="ACHX5T_004152"
     CDS             798..1160
                     /locus_tag="ACHX5T_004152"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol glucosyl transferase"
                     /protein_id="HFR8469096.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            1157..2074
                     /locus_tag="ACHX5T_004153"
     CDS             1157..2074
                     /locus_tag="ACHX5T_004153"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR8469097.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRTERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTIVFGNPVRGYPSMLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPRYLIKSRK"
     gene            2087..>2462
                     /locus_tag="ACHX5T_004154"
     CDS             2087..>2462
                     /locus_tag="ACHX5T_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HFR8469098.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICM"
BASE COUNT          727 a          478 c          533 g          724 t
ORIGIN      
        1 agagagtaaa tcaaaccgcg caattggcgt tgcgctgaat gatactgcat gtcgcgtatt
       61 gaaaaaacaa atcggaaatc atcaccgttg ggtatttgtg tacaaggaaa gctgtaccaa
      121 accagacgga acgaaagcgc caacagtaag gaagatgcgg tatgacgcaa acacagcctg
      181 gaaagcggcg ctgagacggg ctggtattga tgatttcaga tttcacgact tgagacacac
      241 ctgggcaagt tggctggttc aagccggagt cccgttatca gtgttacagg aaatgggagg
      301 ctgggagtct atcgaaatgg ttcgtcgata tgctcacctc gcacctaatc accttaccga
      361 acacgcacgg caaatagact tgattctgaa cccatcggtc ccaaatttgt cccagtcaag
      421 aaataaggaa ggtactaatg atgtgtaact tattgattta aatggtgccg ataataggag
      481 tcgaacctac gaccttcgca ttacgaatct gtagcaccaa tcataactat ctgttttagc
      541 aagcattaac cgcattcact aagatatagt tgatggcaca aacagaaagt tgatgaatga
      601 tgttgccata tatatgtcac aaatacggca caacgatctt caaacatata gccacacatt
      661 cacagaagag cacaaagcct tgcaatccag tgcaaagatt tgtgtgccag agtttttctc
      721 atcaactacc gcaagtatcg atcgattgag acttggatga tagacttcat gcctttgatt
      781 attagctgat agaagaaatg ttaaagctat ttgcaaagta cacatctata ggcgtgctga
      841 acacacttat acactgggtg gtttttggtg tatgtatcta tgccgcgcat accaatcagg
      901 ctatggcaaa cttcgcaggt ttcgttgtgg ctgtgagttt tagcttcttt gcgaatgcaa
      961 aattcacatt caaagcatca actacaacga tgcgctacat gttatatgtc gggttcatgg
     1021 gaacactgag tgctactgtt ggatgggttg ctgatagatg ttcacttcct ccaattgtca
     1081 ctcttgttac cttctccgcc atcagcttgg tgtgcggttt cgtctattca aagttcattg
     1141 tctttaggga tgcgaaatga agatatctct tgtcgttcct gtcttcaatg aagaagaagc
     1201 gataccaatt ttttataaaa cggtacgtga attcgaagag ttgaagccat atgaagtaga
     1261 aattgttttc ataaatgacg gaagcaaaga cgctacggag tcaatcatta acgctctggc
     1321 tgtttctgat ccgctagttg ttccgctgtc atttacacgc aactttggta aagaaccagc
     1381 attgtttgca gggttagacc atacaaccgg cgatgccgtg attccaattg atgttgacct
     1441 gcaagacccg attgaggtta tccctcatct tattgaaaag tggcaggcag gtgctgacat
     1501 ggtgcttgct aaacgttcag accgctcaac tgatggacgg ctgaaacgta agacagctga
     1561 gtggttctat aaattacaca acaaaataag caccccaaaa atagaggaaa atgtcggaga
     1621 ttttcgactc atgtctcgtg aggttgtgga gaacattaaa ctattgcctg agcgcaatct
     1681 tttcatgaaa ggcatactga gctgggttgg tggtcagaca gatgtcgttg aatatgtacg
     1741 cacagagcgt gttgctggca tctcaaaatt taatggctgg aaattatgga atctggcact
     1801 ggaaggtatc acaagttttt caacctttcc tcttcgcgta tggacttata taggcttgtt
     1861 tgttgcaagc atttcatttt tatatggtgc atggatgatt atagacacca ttgtctttgg
     1921 taacccagta cgcgggtatc cctccatgct tgtatcaata cttttcctgg gtggagtgca
     1981 actgatcggg attggtgttc tcggtgaata tataggtaga atttatttag aaacaaaatc
     2041 aagaccacgg tacttaatta aaagcagaaa ataacaggat gatcttatga agatattcat
     2101 aatgcgcaat tggcacaaga tatcaatttt tatacttgca tttactttga tttggcttag
     2161 acgcattgac attttgacaa atgcccagtt ttgggctgag gatgcagttt tttggtataa
     2221 agatgcatac gaacaaggtt ttttcagttc actcataacc ccaagaaatg gttattttca
     2281 aacagtatca acactcattg ttggtgcgac gacatttatt aatccaattt acgcaccatt
     2341 attatcaaac ttttttggaa taataattag agctataatt atatggttct tatttaccga
     2401 tagatttaaa ttcctaagca ccacatcaaa aatcttcatc tctgtatatt taatttgcat
     2461 gc
//
LOCUS       DAZSIO010000309         2441 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZSIO010000309 DAZSIO010000000
VERSION     DAZSIO010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.314"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            65..574
                     /gene="eco"
                     /locus_tag="ACHX5T_004155"
     CDS             65..574
                     /gene="eco"
                     /locus_tag="ACHX5T_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR8469099.1"
                     /translation="MKKESKKMKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     TGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAEVR"
     gene            complement(696..2320)
                     /gene="mqo"
                     /locus_tag="ACHX5T_004156"
                     /pseudo
     CDS             complement(696..2320)
                     /gene="mqo"
                     /locus_tag="ACHX5T_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
BASE COUNT          593 a          656 c          593 g          599 t
ORIGIN      
        1 aagcaaatta ataaatctga tgaatatgtt aaccttcagc gacatcatcg gtgaaaacct
       61 ataaatgaag aaggaaagca aaaaaatgaa gaccattcta cctgaagtat tgtttgccgc
      121 tttcgctacc acttccgcct gggcggcaga aagcgtccag ccactggaaa aaatcgcgcc
      181 ttatccacaa gctgaaacag gcatgaagcg tcaggtgatt cagttaaccc cgcaagaaga
      241 tgaatctacc ctgaaagtag aactgttaat cggtcagacg ctggaagtcg attgcaattt
      301 gcatcgtctc ggcgggaagc tggaaagcaa aacgctggaa ggctggggct atgattatta
      361 tgtctttgat aaagttagct cgccggtttc cacgatgatg gcctgcccgg atggcaagaa
      421 agagaagaaa tttgtcaccg cgtatctggg cgatgctgga atgctgcgtt acaacagcaa
      481 gctgccgatc gtggtgtata cgccagacaa tgtagatgtg aaataccgcg tctggaaggc
      541 ggaagagaaa attgacaacg cggaagttcg ctaaactgtt gtgaagtacg gcaccccgtt
      601 ggttggataa ggcggttacg ccgcatccaa catctaacgc ccgagccggt tgcctgatgc
      661 gacgctggcg cgtcttatca gtatacgcca catcattaca acgcaatatc cgccacttct
      721 ttttgaacgg gttgcggttt caactgcggt ttcggcgtac tatctgctgc ttgcggtttg
      781 tcgtatttca gccccagcac ttcgctggtg tactgcaact cgcgttctgt tgccgctaca
      841 tcaccgttca gcttgcgtcc ataagacgga acgatcgctt tcaacgtagc ctgccattgc
      901 gggctggaaa cgcgatcgcc aaatactttt tccagtagat tcaacataat cggcgcggcg
      961 gttgacgccc ctggcgatgc ccccagcagc gccgcaatgg tcccttgctg gtcactgacc
     1021 acttcggtac ccagacgcag tacgccacct ttctcggcat cacgcttgat aatctgcaca
     1081 cgctgccccg cttgccacaa acgccagtcc tcttttttcg cttgcggata gtactctttc
     1141 aacgcttcaa aacgatcctc ttcactcaac atcacctgac tcaccagata tttcaccaga
     1201 tcgaaattat ccagcccgac gtgcatcatc ggcatcacgt tagaggtggt ggtggaactc
     1261 atcagatccc acaatgaacc gtttttgagg aatttggttg agaaggtggc aaatggccca
     1321 aacagcacta cgcgtttacc gtccagaaca cgggtatcga tatgcggaac cgacatcggt
     1381 ggtgcgccaa cggatgcttt accgtaaact ttcgccagat ggcgattaac cacgtctgga
     1441 ttttccgaaa caaggaactg tccgcccacc gggaaaccgg cgtagtcttt cgcttccgga
     1501 atccccgatt cctgtaacag cttcagcgcc gcaccgcccg cgccgataaa gacaaatttg
     1561 gcgcggatgt tctgtgcagt gccgtttttc agatcggcaa cggtaacggt ccaggtgtta
     1621 tcgtcattac gctttagggc gcggacttcg ctgctgagtt gcagcgagaa gttagatttc
     1681 ttctgtaagg aagcaattaa ctggcgggtg atctcaccat agttcacatc agtgccaatt
     1741 tctgttgcgg atcgcgccct tccatcacta acggtgccca ctctttgatc tgcgcgtgat
     1801 cttcagagta acgcataccg cgaaacagcg agctttgttg caacgcggcg taacgggcgc
     1861 gcaggaaatt aacgttatcc tcgccccaga caaagctcat atgcggaacg gtattgataa
     1921 atgaacgcgg agtacgcagc acgccgcgct cgacctggtg cgcccagaac tggcgggaaa
     1981 tctgaaatgc ttcgttaatg gcgactgctt tttcaatact gatgctgcca tcggcgtttt
     2041 gcggggtgta gttcagttcc atcagtgcag aatgcccggt tccggcgttg ttctagccgt
     2101 tcgaactctc ctgcgcgccc ccctccaggc gctccaccat ggtcatcgac cattcaggct
     2161 ccagctcgcg tcaataggtc cccaacgtgg cgctcataat gccgccgcca atcaacagta
     2221 catcagtttc ctgctcctcg gacgctttcg cttttgccgc catcgaaacg gcattaagcc
     2281 ccacggccat cgagaagagc atggcagtca cttttttcat cttgttaatg ccttactttt
     2341 agtcgcttta ttgcaggtga gatttgcgcg gcatcaacgg taacacttaa gtaacaacat
     2401 ttaaataatg tttataaatt atattctaat tttagaaaca t
//
LOCUS       DAZSIO010000310         2440 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZSIO010000310 DAZSIO010000000
VERSION     DAZSIO010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.315"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..565
                     /locus_tag="ACHX5T_004157"
     CDS             <1..565
                     /locus_tag="ACHX5T_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8469100.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            523..1200
                     /gene="bglJ"
                     /locus_tag="ACHX5T_004158"
     CDS             523..1200
                     /gene="bglJ"
                     /locus_tag="ACHX5T_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR8469101.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="ACHX5T_004159"
     CDS             complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="ACHX5T_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR8469102.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2167..2403
                     /locus_tag="ACHX5T_004160"
     CDS             2167..2403
                     /locus_tag="ACHX5T_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR8469103.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          629 a          534 c          627 g          650 t
ORIGIN      
        1 agtcattgct gatttagccg gtgaaagtga agatccacgt tctgtttgtg aacattatta
       61 ttctttaatc tcacaatatc gggaaattca ctgggttttc atggtgtcac gctcctggta
      121 ttcccaggct gtagaactgc tcatgtgccc tacggcgacg ttattgtctg atgttgaacc
      181 cattgagaat ctggtcaaga ccgtacgttc cggcaatacg cacgcagagc gtatcagcgc
      241 catgctgacc tctccggcaa tgactgaaac tcatgatttt agctatcgct ccgtcattct
      301 cactctttca gagcgcaagg tactgcggct attaggtaaa ggatggggca tcaaccagat
      361 agcttcattg cttaagaaaa gtaataaaac tatcagcgcc caaaaaaaca gtgcgatgcg
      421 tcgactggca attcacagca acgctgaaat gtatgcatgg ataaatagcg cgcagggtgc
      481 aagagaactt aacttgcctt ctgtttatgg agatgccgca gaatggaaca cagccgaatt
      541 aagaagagaa atgtcgcact catagaaaaa tgcgtcatga gtagtatcgg tattgagagt
      601 ttattcagaa agtttgcggg caacccttat aagctccata cctataccag tcaggagtca
      661 tttcaggatg ccatgtcgcg gatctcgttt gcggcggtca ttttttcttt ttctgccatg
      721 agaagtgagc gcagagaggg attatcttgc ctgactgagc tggcgattaa gtttccgcgt
      781 acccggcgtt tagttattgt ggatgatgat attgaagctc ggctgattgg ttcattgtcg
      841 ccatcaccgc tggacggtgt attaagtaaa gcgtcaacgc tggagatttt tcatcaggaa
      901 ctctttttgt cattaaatgg tgtacgtcag gcgaccgacc gactgaacaa tcagtggtac
      961 attaaccaaa gccggacgtt aagcccgacg gagagagaaa tattgcgctt tatgtcgcgt
     1021 ggctactcaa tgacacaaat tgccgagcag cttaaacgta atatcaaaac gatccgagcg
     1081 cataaattta atgtgatgtc gaaactgggc gtcagttctg atgcagggtt gttggaggcc
     1141 gaagatattc tgttatgtat gcggcattgc gaagcaagta atgtgttgca tccctattaa
     1201 tccgcatgat gccgggttta cttccccggc agtgctttca tttcagcgta caatagccac
     1261 attgctgcac atccggtaag cgataacgct ggcagcaagt gcggcgcacc agcaggccgt
     1321 cgcgcagtac cacggtacgc cagagtggat tatcttcacc gttcgtgagc gttttctcaa
     1381 aaaagagggc atggcgcagc gattcaacag tagcctcgcc gagcagttgc ttcatctcag
     1441 tgagatacca gttgatcaaa taaccggtat tactccagat aagtttgccg ttgatctctc
     1501 cggtcgcttc tagtgcttgc acaaccggaa ccagcgcctg gctgattaac gtttccattc
     1561 gctgctgcgg cgaatgtggt gttgcgtttt tatcttcaca cacatcgacc cagaaacagg
     1621 cgacgcgtcc ggtttcgtga aactcagcat ggaaatgttc cggcgacaca tctaatgctt
     1681 tttcctgcgt cagtagcgcc agcattaatg gtggcaccat caggccgata taccattgtg
     1741 cccatagtga gatcagcggt ttgttctcgc ggatcatcgt tggttggttg cgatagatat
     1801 gatcggaata gaccgccagt agagaactta gcgcattcgg tgatgaccat tgcgccagcg
     1861 tcatggcgtt aagcggagcg ggttcatcca ggcggataaa ctccagcaaa tgttcacgat
     1921 gtttggcgat catcgcccgt acggcttgtg caagcccagc atcctgcggc tggagatgcg
     1981 ttcgccagat gacatcttca tagagcggtg cggaacgata ggccataatc gtgatagtaa
     2041 tctaaatgat aatgattgct aatcctagcg ataggtttac tcgatagcaa gggatttctc
     2101 tggcttgcaa atgataaaaa ttatcatatg atattggttt tcattatcaa tgaaagagat
     2161 gaaaccatgt tgcaacgtac gctgggcagt ggctggggag tgttgctgcc gggattgctg
     2221 attgcagggc tgatgtatgc ggatttatcg ccggatcagt ggcggattgt cattctgatg
     2281 ggattagtat tgacgccggt gatgctgtat cacaaacagt tgcggcatta cattttgcta
     2341 ccatcgtgcc tggcgcttat tgctggcatc atgctgatga taatgaattt gaatcaggga
     2401 tgacaaaaca gggaagaaac aagaaaggaa gtaaagataa
//
LOCUS       DAZSIO010000311         2436 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZSIO010000311 DAZSIO010000000
VERSION     DAZSIO010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.316"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..360
                     /locus_tag="ACHX5T_004161"
     CDS             <1..360
                     /locus_tag="ACHX5T_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR8469104.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="ACHX5T_004162"
     CDS             361..726
                     /locus_tag="ACHX5T_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR8469105.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1411
                     /locus_tag="ACHX5T_004163"
                     /pseudo
     CDS             723..1411
                     /locus_tag="ACHX5T_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
     gene            1605..1680
                     /locus_tag="ACHX5T_004164"
     tRNA            1605..1680
                     /locus_tag="ACHX5T_004164"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1638..1640,aa:Ile,seq:cat)
     gene            1682..1758
                     /locus_tag="ACHX5T_004165"
     tRNA            1682..1758
                     /locus_tag="ACHX5T_004165"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1716..1718,aa:Lys,seq:ttt)
     gene            1761..1836
                     /locus_tag="ACHX5T_004166"
     tRNA            1761..1836
                     /locus_tag="ACHX5T_004166"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1794..1796,aa:Thr,seq:tgt)
     gene            1840..1914
                     /locus_tag="ACHX5T_004167"
     tRNA            1840..1914
                     /locus_tag="ACHX5T_004167"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1873..1875,aa:Gly,seq:tcc)
     gene            2207..2422
                     /locus_tag="ACHX5T_004168"
     CDS             2207..2422
                     /locus_tag="ACHX5T_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR8469106.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          640 a          520 c          653 g          623 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc gcccggccct ttagctcagt
     1621 ggtgagagcg agcgactcat aatcgccagg tcgctggttc aaatccagca agggccacca
     1681 accgccacta gctcatcagg aaagagcgtc aaccctttaa gttgagtgtg cgaggttcga
     1741 gtccccggtg gcggtccagt gccgacttgg ctcagtaggt agagcaactg acttgtaatc
     1801 agtaggtcac cagttcgatt ccggtagtcg gcaccatatg cgggcatcgt ataatggcta
     1861 ttacctcagc cttccaagct gatgatgcgg gttcgattcc cgctgcccgc tccagtcaga
     1921 gtctttcagt ctgcgatgat gggaaatccc ggagtgactg aaagacgttt aagttatgaa
     1981 tgatcgcttt tttttgcaaa attgctgtgc agaaatacta accttcgggc aggcgatcat
     2041 tcataagcac tctgctttta ttccgattaa ctgtgagtgg tttgttggat agagtgcttt
     2101 ccttattgta tatatcgttt cgcccgcttt tgcggttttt tcttttcaaa tccctttcat
     2161 ttctcagtgt aaaactacgc catccgttat ttgcggaggt gaggctatga aatccatgga
     2221 caaaatttca acgggcattg cctacggcac ctccgcaggc agtgctggct actggttttt
     2281 acagtggctt gatcaggtca gtccgtcaca gtgggctgcg attggtgtac tggggagtct
     2341 ggttctgggc ttcctgactt atctgacaaa tctgtacttc aaaatcagag aagacaagcg
     2401 taaggctgcg agaggtgaat aatgtcgcca tcatta
//
LOCUS       DAZSIO010000312         2412 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZSIO010000312 DAZSIO010000000
VERSION     DAZSIO010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.317"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..545)
                     /gene="ygeQ"
                     /locus_tag="ACHX5T_004169"
     CDS             complement(<1..545)
                     /gene="ygeQ"
                     /locus_tag="ACHX5T_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeQ"
                     /protein_id="HFR8469107.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDG"
     gene            complement(1207..2196)
                     /locus_tag="ACHX5T_004170"
     CDS             complement(1207..2196)
                     /locus_tag="ACHX5T_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469108.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(2280..>2412)
                     /locus_tag="ACHX5T_004171"
     CDS             complement(2280..>2412)
                     /locus_tag="ACHX5T_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469109.1"
                     /translation="REQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWMDSL"
BASE COUNT          645 a          553 c          577 g          637 t
ORIGIN      
        1 attccatcca gtcgttttgg gttgctaatg atgtcctgca tccgcttaat ctggtcgggc
       61 gtagacatat aaccccacag ggtgagcaga accttttttg cggctacact atttccttgg
      121 tctgatacta atgccatata gccccataaa ctatccctca gccagacagc atcatagtgg
      181 gtttctgccg ctgttttgtc atcttcgaaa gtagaagcaa gaataaacgt tccccagggt
      241 ttctccgtca acgttaactt tcgtgtgttt tctgttaatg ttagcaatcc ttgagtttcg
      301 tctttggtat agtgactctt tatttcattt tttaatttgg ctgagtgaat cagaagacga
      361 taattaggat acttatctgg atttaacgat aaataacttt tatctgttgc tgcccccctg
      421 gaattaagaa tgttaaatac agattcgctt gtcacttctg cctctgtcgc ctggcatgtg
      481 ccgcacgaaa atataccagc gagcagaaga gttaatgcag atttcccttt cataaaataa
      541 tccatcaatc gtttgttaaa agcgtagata accacttatt caacaacttg aaaataaggt
      601 attttacctg agttgttaca aaaggattgc attgcgtaaa cgctttttat ttacaaaaaa
      661 atggggaagt gttacagcga ttgcgaaaag cctgaagtga ttgaattaag ataatataga
      721 aaatcagata cagcaaaaat ggcagattga tcaggtgaag ataaattgga gcgggcgaag
      781 ggagcctacg acaaatcgaa gacaaccagc taacctattg tttcttaaag acaggtgaat
      841 ccagttggtt gtagtgatag tcacacattt agagatagaa ggtcaagact tgaagcacgt
      901 aatattccct ctaaaggaat agaactcacg gtctgactac ccggcccttt tggtctggct
      961 caggtcaggc tatgggggct tttggctcgc tctttatatt agatgaggtc tcacattttt
     1021 cagtcaaaac tcatcgtcta atagcccata gccagaccac cagtcccagc tttagcagga
     1081 agaccacgag atagactttg tgtaggtcca tttggccctc ctactttctg tattcacaaa
     1141 ctagattact agtacaaaat aaaggccaga catccagttc ggaagtctga ccaatagtcg
     1201 ttattgttag ttagcttctt tagtgaagct caggcgtttg actacaggca ggacagcagt
     1261 agccgagcca gtacccatct tgatggtcgt agcagcttca gctacaacaa acaccttgcc
     1321 gagaatctca aggtcgtgag actgggagtc tccatcacgt gaaaggacca cagtaatgct
     1381 ctcgccatcg tgacggaagc aaccgaagct cagagccgta aaacgcttag ccatcatgaa
     1441 cacgccacgg tcttcaccgt tgtaataggc gacgtcagtc cccagcatat cgacgatagc
     1501 gtcttcacca ccgaagccct gcttagcagc cagacgggag agagccttga ggtgtgactc
     1561 atgggcgata atgccgtagt cagaaacctc agaaccgaaa gaagactccg tgttctcgta
     1621 cagggcatcc atgatagcgt cagccttagc ggtcaggcta ccagtcaggg ccggaagttc
     1681 tacggtcttc agcagctttg aggactccag cacgtcaacc atcttagagt taatggtacg
     1741 aatcacgtta tcgcagatgt tcagtgcgtc ctgctggagc gtcccctgag tgaaaccagt
     1801 ggtcgacttc tggtcccaaa gctttgtttg gtgagacacg aggatcgcct gttcaatcgg
     1861 ctcaacggtg atttcagtgc cagacggagc ggtcacaact tcaggtcgct taccaccacg
     1921 gacagggacc agcacagagc ggtcagccag agtgtgagca atgattggag tcggtaagct
     1981 gttacgggtc ggcaggactt ctacagggaa cgggatgtta tcttgagcgt ctttaagtac
     2041 accgttcagc atagccttaa cagtcttagc taactggagg gcatagacct gagggtcggc
     2101 gttagtcggc agaccttcat tgacctgaat gttgtcggta tcggtgatgc gtactggctc
     2161 aatgccaaac tggaaagcgt ttttagtcat attcattata attaatctcc ttgagtcaaa
     2221 tacttgaagt ccctaacagg cagtcaggga ccgtgtgttc tcagatgagg gacgcttgct
     2281 tacaagctgt ccatccagtt accgcctgac tcaaggtcag cgacgatagc ggcacgtgag
     2341 cgaccagcgg aatgagtagc gctgcggagg ttttcaccat aggtccggcg ttcagctttg
     2401 acctgttcgc gg
//
LOCUS       DAZSIO010000313         2359 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZSIO010000313 DAZSIO010000000
VERSION     DAZSIO010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.318"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="ACHX5T_004172"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="ACHX5T_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR8469110.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1861)
                     /gene="phoA"
                     /locus_tag="ACHX5T_004173"
     CDS             complement(446..1861)
                     /gene="phoA"
                     /locus_tag="ACHX5T_004173"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR8469111.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="ACHX5T_004174"
     CDS             complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="ACHX5T_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR8469112.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
BASE COUNT          531 a          608 c          554 g          666 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagcttgt tgcgttgctt gattattaca ttcacgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cagttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca agggttcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca tcggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt tttttattca gcgcatagtg agtgtattgc ccggtaagcg
     1561 gtaaggcatc gataccttta aaaaagccgc ccgcaccttc ggcataatta cgtgcggcag
     1621 taatttccga atcccccatc ccatcgccaa tcagcaaaat aatatttttt gcaggtttat
     1681 cgctaagaga atcacgcaga gcggcggtct gatcacccgt taagcggcga gcaccgccgg
     1741 gtgcagtaat atcgccctga gcagcccggt tttccagaac aggcatttct ggtgtccggg
     1801 cttttgtcac aggggtaaac agtaacggta agagtgccag tgcaatagtg ctttgtttca
     1861 ctttattttc tccatgtaca aatacattaa aaaataaaaa caaagcgact ataagtctcg
     1921 gccgtgacaa ctttatgaca gctgttgaaa agattaactt tttactgaca aggatgcttc
     1981 aataacttct ttacgtaatc gcgcagcagc tctgtatcgt cgtcaggaat gctggcatcg
     2041 ggctttacct cgtacagcgc cccctctacc tgatcaatca accgctgttg gtcattttgc
     2101 gccatattgc gaagcattgc agtgacgata atctccaaag cttctacctg cgcacacagt
     2161 tctttcgact cttcttcttt ttgggcaagc ttaaataaca actcagcaat gagatttttc
     2221 atgtctgtat ttccttatcc aaagtatgga gaagttaaca ttatgtttca caacttcata
     2281 gtgtctggaa gtattatttt tatgacaaag gaaatacttt gcgcaaatat tttttgtttg
     2341 attgccagcg aaacgtttc
//
LOCUS       DAZSIO010000314         2325 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZSIO010000314 DAZSIO010000000
VERSION     DAZSIO010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.319"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            61..282
                     /gene="kil"
                     /locus_tag="ACHX5T_004175"
     CDS             61..282
                     /gene="kil"
                     /locus_tag="ACHX5T_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR8469113.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYILHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            282..452
                     /locus_tag="ACHX5T_004176"
     CDS             282..452
                     /locus_tag="ACHX5T_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFR8469114.1"
                     /translation="MTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYCSE
                     ICAEKDQMAHEL"
     gene            527..802
                     /locus_tag="ACHX5T_004177"
     CDS             527..802
                     /locus_tag="ACHX5T_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469115.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            902..>2325
                     /locus_tag="ACHX5T_004178"
     CDS             902..>2325
                     /locus_tag="ACHX5T_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFR8469116.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKTQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAE
                     LHEARKNIPPKNPVDADKLLAALR"
BASE COUNT          720 a          531 c          528 g          546 t
ORIGIN      
        1 taagtattaa ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc
       61 atgattgctc atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
      121 tatattcttc atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa
      181 ctttatattc gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc
      241 ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt aatgactaag aaaataaaat
      301 gtgcttatca cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc
      361 atttcatgcg tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg
      421 ccgaaaaaga ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat
      481 gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta
      541 tgaagccact gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg
      601 tattggctac gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga
      661 cggtccatca ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca
      721 cataggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat
      781 gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa
      841 gaacctggga catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac
      901 aatgaacacc gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag
      961 aaaacgtctc ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc
     1021 catctcccgt ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa
     1081 acctgttcgc gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac
     1141 ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc
     1201 atcttctccc tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga
     1261 agacatgaca gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact
     1381 ccaggcatta cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc
     1441 ccacaaaacc agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga
     1501 tactgacaga gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt
     1561 cgaagcatac atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa
     1681 cctcaccgat cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc
     1741 cactggcgta ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc
     1801 taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1861 attcatcacc atgcctggcg ggatggatta ttcccgcgcc atcgtggttg cgtcagtgaa
     1921 agaagcaccg attggtatcg aggtcatccc cgctcacgtc actgaatatc tgaacaaggt
     1981 actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg
     2041 ctcctctgcc ccgatgccgc aacgtgtaac agaagaagga aaacaggatg atgaagaaaa
     2101 aacgcagcca tctggcgcaa tggcagatga acaggcaacg gctgaaacaa tggaaccgga
     2161 tgcaactgaa catcatcaga acacgcagcc gctggatgct cagtcacagg taaattctgt
     2221 tgatgagaaa tatcaggaac tacgggcaga actccatgaa gcccggaaaa acattccgcc
     2281 caaaaatcct gtcgatgcag acaaattact ggctgccttg aggta
//
LOCUS       DAZSIO010000315         2303 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZSIO010000315 DAZSIO010000000
VERSION     DAZSIO010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.320"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(586..2190)
                     /locus_tag="ACHX5T_004179"
     CDS             complement(586..2190)
                     /locus_tag="ACHX5T_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8469117.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          596 a          555 c          507 g          645 t
ORIGIN      
        1 ttaatcttta attgtgtctt taagttaact ttaagttaag cccctgtagg gactagagac
       61 cataattaag atgattgtgt cttattgcta ttcacttatc gactgaccca gagcacacag
      121 gaagcattca ctttaagtag aacgcttggt gtctgtaatt ggtcatcccg cccctgaaag
      181 gatgcgataa gttacggtta gttgtcctag aagagaaaac tttttgtgtc ccttctccct
      241 atagtgagtc gtattaatta tccttgacaa gtagagaaaa ccatgagtca aaaacatgac
      301 cacacaggtc tgtagtaagt gtcatcctag aagagaaaac tttttgtgtc ccttctccct
      361 atagtgagtc gtattaatta ccccttgaca agcagagaaa actatgagtc aaaaacatga
      421 ccgcacaggt ctgtagtaag tgtcatccta gaagagaaaa ctttttgtgt cccttctccc
      481 tatagtgagt cgtattaatt atcccttgac aagtagagaa aactatgagt caaaaacatg
      541 accacacagg tctgtagtaa gtgtcatcct agactatggt ctcctctagg tctccttata
      601 aacatcagcc atcacattga ccagcctatc aacctcgacc tctttagcat aacggtcgta
      661 cgtaatagac cctgaactat gccccatgat cgcctgtgca tacgccaact cgaccccatg
      721 ttgttttaac tgggccgcaa ctgtatgtct aaacgagtga aacgctaagc cgctacggtc
      781 tacgaggact ttaggcaaca acgtccgatt aaaccattta ctcacctgct caccgtaacc
      841 gttcttcatg agacgcagac catcgaacac catagcgtca tcaccgtcag cgcctctacg
      901 ggtctctaca agcgccataa agtcagccaa tataaaacca taggccccat cgaccagagg
      961 cacacagcgg tcactatgag cgttcttgat gctcttaccg ggtagactac tatcgtcttc
     1021 attgatatga atataaacgg ttcccgcctc agtggtcctg acgtctttga cctgtagttg
     1081 ggctatttca ttgagtctcg ccccagtgat agcagccaag gcggtaacat aataatgata
     1141 cggcttacca gaggtcttct gagagtatgc cttagctgcg accagtagtt gccccacctg
     1201 ctccgtagag aaggcgttac gggcccctga ggctttacgc tgagggactt taagctccag
     1261 accctcagtc atgttctttt taatcaagtc attgcgcaca gcccacctaa agacagcagc
     1321 catcttaata agatacttgt tgttgatggt aacgacatcc agacaatctg tcttatcttc
     1381 acggctcaac aggtcagcaa gtgggacatc cttgaagcgc tgcttacggt tcctaggtaa
     1441 ctgctggagc acatcacgga ccctaagcat gtcagccctg ttagcgtctg cattgagacc
     1501 cagatggtcg aatatttcga ttaacgcagc gtgtgacgcc ttgttttccc ttaaggttgc
     1561 aggcttccag ttctgatagt tctccgcttc gtattgttca aagaggctcg ctatggtaat
     1621 gctaggcgta acttctggtt ttacttctgg agcattaaca gatagggaca cagagtctgc
     1681 ttggttatct tgcctatcaa tatcattaaa aagatcgacg taatctatca gaggctgact
     1741 gtctcccgct tcgatacgcc tcatacaggc ttttagcacc ttcagggcct cattgatgta
     1801 acgatgctgg tcaatgctta gcggctcact agccagagca tcggtaaggc tctcaccaag
     1861 ttctccgtat tggtcccggt agatatccct catgtcaggc tcaaagagat ctgaacggcc
     1921 catagacagt tcacactcag cgatgtcctt aaggtgttcc ctcatttcct cataggtagc
     1981 gttgggtctg tctgcgtgta ttgccttgag tgctgccttt atgtgccttg agtaagccat
     2041 agcggtgcgt ctatccttgg tccgtaatga tattgaggtc attctatcat tgtttgattg
     2101 tctgacccta agacggaagt agtagacgcc gttacgctgg tagagatagt agactgctgg
     2161 aagtttgtga ctactggata gctgaatcat atgtctgcat gccctgagag ggttacacag
     2221 tgggttacag taagtaactc tgcaaggagg cttaaagccg tatcccttag gattcagtag
     2281 gttggagtac tttaagaaag gtc
//
LOCUS       DAZSIO010000316         2283 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZSIO010000316 DAZSIO010000000
VERSION     DAZSIO010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.321"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..106
                     /locus_tag="ACHX5T_004180"
     CDS             <1..106
                     /locus_tag="ACHX5T_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR8469118.1"
                     /translation="VAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(195..707)
                     /locus_tag="ACHX5T_004181"
     CDS             complement(195..707)
                     /locus_tag="ACHX5T_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR8469119.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            902..1666
                     /locus_tag="ACHX5T_004182"
     CDS             902..1666
                     /locus_tag="ACHX5T_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469120.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1617..>2283
                     /locus_tag="ACHX5T_004183"
     CDS             1617..>2283
                     /locus_tag="ACHX5T_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR8469121.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          653 a          497 c          578 g          555 t
ORIGIN      
        1 agttgctgga cgaaacgttc tcggtatccg ggacggaatc atcagcgacc agacagcatt
       61 gagaatgctt caggagtaca tcaggatcca atgccttggg gggtagcggt aattttactc
      121 attatccttc aaatcagatt ctgttgtcag aggaatgggg gaagctggat tcgcaagagc
      181 tttttatggg cagtttagct aaaaaaaggc aacacgaagc taaaactacc aacataaagt
      241 gctgaccagc tagcagctga aatcagactc actgtataca ctttcactgg atgaagttgc
      301 aacattcctg ccaccaaagg agcaatgtaa cgcagcacgg cgataaagcg cgaagtgaaa
      361 agtattgaca cagagttatt ttgcaactga aggcgaaccc gttctattgt attggaacgg
      421 ctcgatatta tacatgcaag ttgagggata tgcccaatta ttataccaag atggtaatta
      481 acgatagtac cgcaccatgc gccactcatg accgtgattc cagcttccca tggtgaaaga
      541 gtcgtcttgc tgactgtaat tacagtggcc atcataattg atgctggtgg caatatcgcc
      601 gaaatgagta aagtggattt tgtgaacgct attataaaaa gaagccccca taggcgatat
      661 ggatgaagta caaaatagtt catcaaagca tttatccact ccatcaatgt ctttccttct
      721 gtgaattttt ctttgctatt taaccgtgat acgacttaat ttaggcttaa acaccttaca
      781 tttacaaggt actcctgtgg ggggccctgc cacggagcgt cgcaggagtg agtgatgagg
      841 aagaattaaa gcaacctatt gtgacaggca gcgataacag taaatcacag gaagcataat
      901 catggcaaaa ccggactggg aggccatcga atcggcatac cgggccggag tccttagcct
      961 ccgtgatata ggcgagaaat acggcgttac agaaggggct atcaggaaga gggccaaaaa
     1021 gcttgattgg gcacgcagtg gcggtacgca ggtttgcaaa aatggtacgc aaaaaaggaa
     1081 agtgcgtacc agcagaaagc ctgccattac tggccttaca caaaaaagta cgcaactaaa
     1141 aacagaatct acaccggata cgaaaccgat acgcggaatg cgtaccgatc ccccgactaa
     1201 cccattccaa cccggtaacc agcaggcatt aaaacacggt ggttatgccc gtcgccttct
     1261 gctcaaagat gaggtgatag aggacgctaa agcgttgacg ctcgaggacg aattatttcg
     1321 ccttcgtgct aacaaccttg tcgctgcaga gaatattggt cggtggttgg tgtcgctgga
     1381 agatgctaat ggggaccagg aaaggaagat gctgattgaa aatatcagcg ccgccgagaa
     1441 agcaatgatg cgcaatacag ttcgtattga gtccatcgtt ggcacgcttg cgacggtagg
     1501 aaaaatattt gctgatacag cctaccgcaa ggccgccact gataaggtgt ctctggaggc
     1561 tgatcgtctt cgccgtgatg caggtattga tgatggcaat ggagagcgtg acctcaatga
     1621 cttctactct gacatccaaa ccgacgctga atccggcttt acgtagtttc tggactatgc
     1681 gggcacgtaa caaagtgctt tatggtggtc ggtcatcgtc aaaatcatgg gatgccgctg
     1741 gcattgccat atttctgtcg aataaataca ccctgcgttt ttgttgtgcc cgtcagatcc
     1801 agaataaaat cgaagagtcg gtgtataccc tgctcaaaat tcagatagac aggtttggtc
     1861 tgcggcaccg tttccgtatt ctgaacaaca aaatcattaa ccgggttact ggctcggaat
     1921 ttgtttttta tggattatgg cgcaacatcg aagaaattaa gtcactggag gggatcgata
     1981 tgttgtggtt ggaagaagcc cacgcactga cggaatacca gtggaaaatt ctggagccaa
     2041 cgatccgtaa agagggttcg gaatgctggt tcatattcaa ccccggactt gttactgatt
     2101 tcgtctggcg caacttcgtt gttgatccgc ccgaaggcac tctcatccgc aaaattaact
     2161 atgacgaaaa tccgtttctg tctgacacca tgcttaaggt tatcgacgcg gcgcgacgcc
     2221 gtgatccgga tggttttaaa catgtgtatg agggcgttcc ggagtctgat gatgatgcgg
     2281 caa
//
LOCUS       DAZSIO010000317         2281 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZSIO010000317 DAZSIO010000000
VERSION     DAZSIO010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.322"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..1063
                     /locus_tag="ACHX5T_004184"
     CDS             <1..1063
                     /locus_tag="ACHX5T_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inositol phosphate phosphatase SopB"
                     /protein_id="HFR8469122.1"
                     /translation="EYNGKGICCASTRESDHIANMWLSKVVDDEGKEIFSGIRHGVIS
                     AYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKIVSTSLLTPTSL
                     TGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVVTFNFGVNELAL
                     KMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKNDVIYLANQIKE
                     IVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQDAEIKREIIRKH
                     ETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKKLPLSSLELSYS
                     ERIGDPKIWNMVKGYSSFV"
     gene            1073..1435
                     /gene="ipgE"
                     /locus_tag="ACHX5T_004185"
     CDS             1073..1435
                     /gene="ipgE"
                     /locus_tag="ACHX5T_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HFR8469123.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            1435..1893
                     /gene="ipgF"
                     /locus_tag="ACHX5T_004186"
     CDS             1435..1893
                     /gene="ipgF"
                     /locus_tag="ACHX5T_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HFR8469124.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            1902..>2281
                     /gene="mxiG"
                     /locus_tag="ACHX5T_004187"
     CDS             1902..>2281
                     /gene="mxiG"
                     /locus_tag="ACHX5T_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HFR8469125.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSV"
BASE COUNT          802 a          289 c          529 g          661 t
ORIGIN      
        1 agagtataat ggaaaaggaa tttgttgtgc ttctacgaga gagagtgatc atatcgcgaa
       61 tatgtggctt tcaaaagtgg tggatgatga ggggaaggaa attttttcgg gtatcagaca
      121 tggagttata tcagcctatg gattgaaaaa aaactcatct gaaagagctg ttgctgctcg
      181 taataaagct gaggagttag taagcgcagc attatatagc agaccagagt tattatcaca
      241 ggctttgtct ggtaaaacag tagatttaaa gattgtttcg acttctcttc tgacgccgac
      301 cagtttaacc gggggagagg agagtatgtt aaaagatcag gtgaacgcat taaaagggtt
      361 aaactctaaa agggggaagc caacgaaatt attaattcgg aatagtgatg gtcttctgaa
      421 agaagtaagc gttaatctaa aagtggtgac atttaacttt ggtgtaaatg aattagcgct
      481 aaaaatgggc ttaggctgga ggaatgttga caagcttaat gatgaatcaa tatgttcttt
      541 gttgggggat aatttcctca aaaatggtgt gattggaggt tgggctgcag aagcaataga
      601 aaaaaatcca ccatgtaaga atgatgtgat atatctggct aaccagataa aagagattgt
      661 aaccaaaaag ttacaaaaaa atgataatgg agagccgtat aaattgtcac aaagaatgac
      721 tctattggct tatactatag gtgctgtacc ttgctggaat tgtaagagtg ggaaggacag
      781 aacaggtatg caggatgctg aaattaaaag agaaataata agaaaacatg aaacaggtca
      841 gttttctcaa ttaaatagta aattatcctc agaagagaaa agattatttt ctactattct
      901 aatgaatagt ggtaatatgg aaatccaaga gatgaatact ggtgtgcccg gaaataaagt
      961 catgaaaaaa ttgccgctat cttcgttaga gctatcttat tctgaaagaa taggggaccc
     1021 aaaaatatgg aatatggtga aagggtattc gtcatttgta taagaggaat atatggaaga
     1081 tttagcagat gttatttgcc gagccttggg tatcccttta attgatatcg atgatcaagc
     1141 aatcatgctt gatgatgatg tgctaatata tattgaaaaa gaaggagatt caataaattt
     1201 attatgccct ttttgtgcat tgcctgaaaa tataaatgat cttatatatg cgttgagcct
     1261 aaattactca gaaaagatat gcttagctac tgatgatgaa ggcggaaatc taattgcgcg
     1321 attagatctt accgggataa atgaattcga ggatgtatat gttaatacag aatattatat
     1381 ttcgcgtgtg cgttggctga aggatgaatt tgcgcgaaga atgaaggggt attaatgtcc
     1441 cgtttcgtat ttattttgct gtgttttatc ccatatttag ggagagctga ttgttgggat
     1501 aaggctggtg aaaggtacaa tattccatca agtttgttaa aagcgattgc ggaaaaagaa
     1561 tccggattta ataaatctgc agtcaatgtt aataacaatg gaagtaaaga ttatggtata
     1621 atgcaaataa atgactttca ttctaaaaga cttagagaaa tgggatattc tgaggaaatg
     1681 ctaattagtc atccatgtct ttctgtacat tatgcagcaa agttattaaa tgagtttatg
     1741 atgatgtatg gaagagggtg ggaggctgtt ggagcctata atgcaggaac gtctccgaaa
     1801 aagaaaaaag aaagattaaa gtacgcagag gatatctata gacgctattt gcggatagca
     1861 gcagagagca agcagaataa tcgaaggata taagaggatt tatgtctgag gcaaagaact
     1921 caaatcttgc accattcagg ttattagtga aactcacaaa cggtgtcggg gatgagtttc
     1981 cgttatatta tgggaataac ttaattgttc tcggacggac gatagaaaca cttgagtttg
     2041 gaaacgacaa tttcccagaa aatattattc ctgtaacaga tagtaaatcg gatggtataa
     2101 tttatttgac cataagcaaa gataatatct gtcaatttag tgatgaaaaa ggtgagcaaa
     2161 ttgatatcaa ttcacaattt aattcatttg aatatgacgg gatttctttt caccttaaaa
     2221 atatgaggga ggataaatcg agaggccata tactaaacgg aatgtataag aaccactcgg
     2281 t
//
LOCUS       DAZSIO010000318         2260 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZSIO010000318 DAZSIO010000000
VERSION     DAZSIO010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.323"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..242)
                     /locus_tag="ACHX5T_004188"
     CDS             complement(<1..242)
                     /locus_tag="ACHX5T_004188"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469126.1"
                     /translation="MFFSLSVLNLEDHLGLVISPQSPLSAGYITLRACRRSLPLRFST
                     VTPGGIVGFLIVSYRFNIPDRLAVHLSIHARPELRA"
     gene            671..2179
                     /gene="ltrA"
                     /locus_tag="ACHX5T_004189"
     CDS             671..2179
                     /gene="ltrA"
                     /locus_tag="ACHX5T_004189"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR8469127.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          574 a          559 c          668 g          459 t
ORIGIN      
        1 tgtgcccgga gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata
       61 ttgaaccggt aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac
      121 ctgagcggca gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat
      181 tgaggcgaga tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac
      241 atgaacccgt taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac
      301 aggccgtgca ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac
      361 atgtatgcga aacaccgggg cagcagcgtc tatcacactt gcgttgctga cttctgccaa
      421 cttgcggcaa gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag
      481 gcaggtaaac cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc
      541 tggctggcgg agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga
      601 agcagtttga atgtgtttgc gacgtgaatt aactgaccta acgaggtgaa gacctttgat
      661 aatcagcgaa atgcaacgca agcttgccac atgggcagcc accgatccgt ccctacggat
      721 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
      781 gctttcatca aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      841 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      901 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      961 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
     1021 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
     1081 cgcgatccgc acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt
     1141 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
     1201 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
     1261 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
     1321 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
     1381 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
     1441 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
     1501 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
     1561 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
     1621 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
     1681 tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc
     1741 cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga
     1801 acaactcaac cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc
     1861 caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg
     1921 caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg
     1981 tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat
     2041 actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa
     2101 tccctatctg aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat
     2161 ggcgttcgcc agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg
     2221 ggaggagagg cagggaaata gtccgactac gccctgcctc
//
LOCUS       DAZSIO010000319         2218 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZSIO010000319 DAZSIO010000000
VERSION     DAZSIO010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.324"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="ACHX5T_004190"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="ACHX5T_004190"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR8469128.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="ACHX5T_004191"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="ACHX5T_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR8469129.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..2216)
                     /gene="arsR"
                     /locus_tag="ACHX5T_004192"
     CDS             complement(1881..2216)
                     /gene="arsR"
                     /locus_tag="ACHX5T_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR8469130.1"
                     /translation="MQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISRH
                     LALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQNC
                     SGDSKNICS"
BASE COUNT          589 a          611 c          555 g          463 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgg
//
LOCUS       DAZSIO010000320         2187 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZSIO010000320 DAZSIO010000000
VERSION     DAZSIO010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.325"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(220..384)
                     /locus_tag="ACHX5T_004193"
     CDS             complement(220..384)
                     /locus_tag="ACHX5T_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469131.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1286..1570
                     /locus_tag="ACHX5T_004194"
     CDS             1286..1570
                     /locus_tag="ACHX5T_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR8469132.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1558..2043
                     /locus_tag="ACHX5T_004195"
     CDS             1558..2043
                     /locus_tag="ACHX5T_004195"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8469133.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          565 a          462 c          620 g          540 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc
      481 gaaaatgtgc tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc
      541 ggcatccggc atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc
      601 tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg
      661 atgcgagaca caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg
      721 agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc
      781 catgagtcag ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg
      841 ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt
      901 taccaatcga agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt
      961 gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga
     1021 attcgtcacg actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg
     1081 gttcaacgac aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg
     1141 ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta
     1201 aatgccgctg acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg
     1261 tcaatatgct attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca
     1321 gctcaatctc agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct
     1381 tcataagtcg cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat
     1441 tctcgaccgg cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct
     1501 tgatgctccg gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg
     1561 ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc
     1621 agtgaagctg tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga
     1681 gccacccgga cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca
     1741 ctggccactg gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca
     1801 gaccccattc ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa
     1861 gggcttggcg cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat
     1921 atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt
     1981 catcatggtt ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa
     2041 tagggagtaa tgccgatcag ttaaggatca gttgaccgat ccagtggctg tgtaagaatc
     2101 cggaaacgct cactcgtttc cggatttttc tgtgcacagt aaaagaccac ctcctgagga
     2161 ggtggctttt cagaatgccc cctataa
//
LOCUS       DAZSIO010000321         2183 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZSIO010000321 DAZSIO010000000
VERSION     DAZSIO010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.326"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..203
                     /locus_tag="ACHX5T_004196"
     CDS             <1..203
                     /locus_tag="ACHX5T_004196"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469134.1"
                     /translation="RAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVEN
                     VVDVYVTHNPKKTEQKAGVSIH"
     gene            293..694
                     /locus_tag="ACHX5T_004197"
     CDS             293..694
                     /locus_tag="ACHX5T_004197"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469135.1"
                     /translation="MNGNTTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDY
                     LPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSR
                     DGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            696..1301
                     /locus_tag="ACHX5T_004198"
     CDS             696..1301
                     /locus_tag="ACHX5T_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HFR8469136.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1288..>2183
                     /locus_tag="ACHX5T_004199"
     CDS             1288..>2183
                     /locus_tag="ACHX5T_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR8469137.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          531 a          507 c          609 g          536 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtatccat taacacccgg ttcgctttat gttggcgtgt acggcggcag
      241 cccggcagat atcgcggcgg ccatctggcg taaggctccg ccgggtattg atatgaatgg
      301 caacacaacg ttcactgttg cagataagga gtacgatccg ccgtatcctg aatacgtgat
      361 cagctggcag acactcaaac ccgtcagtct gcatgtcagt gtgacgctga aaaaaagtga
      421 ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg catttaatgg
      481 tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc gctactatgc
      541 cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg tgagccgtga
      601 tggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg ttctggatgt
      661 gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct gccactgtgc
      721 ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt aacgcagcgc
      781 tttcccccga cagttttatc agtgattttt atggtcttat ctggaacatc gataccgcag
      841 aaaagtatgg tcttgatgtc tggggaaaga ttgtgggtgt cagtcgctgg ctgacggtaa
      901 aggacgattt taattacctg ggcttcagcg agtccaggat ggacaccccg gtaatggatg
      961 atccctgtcc gtttaatcag gcaccgtttt acaacggaaa atcggatacc cggactgttg
     1021 acctgtctga tgctgtatac cggcggctga tactgatgaa agccatgtcg aacattactg
     1081 actgttccgt tccggatatt aacctgatgc tgagatttat gttcggaaaa aaacgccggg
     1141 cttatgttct gaataatggc gggctgagga tgagttacgt ctttgagtcc gcgctctcgt
     1201 tggcagaact ggcgattatc cagtcgtcgg gtgcactgcc atccccgccg ggtgtttatg
     1261 tttcagtagt tttaaaggag tcccgtaatg aaggccagtg ataaaccccg ccagctggcg
     1321 gtcccctttg cgagtaccgg agataaaaac cgtatcccgg acaaggcgac acagcagacc
     1381 agagagagcg gtaatgctgc gtatgattca ggttttcctc cggtgaccat gacagcggtc
     1441 tcagcgggag gtataccgcc acacggcaag gattttaacg gtctgatgta cgatattacc
     1501 gcagcaatac ggttcgccca ggctggcggt ttgtacacgt ataatgccgg ttttgcgggg
     1561 gccattggtg gatatgcaaa aggagccatt ctcgccggag tcgcaacaac agcggtctgg
     1621 ctgaatacca cggacgataa cctgaccgat cctgaaggct ccgacagtgc gggctgggta
     1681 aatcttcttg aggatccgaa aaggatattc ctgcggcaga agaacaatct gtcagacctt
     1741 cagaataaag ggacggcacg ggataatctt caggtttaca gtaaagagca gtcagatcaa
     1801 cgctatgttc atcgggaagg cgataaaata accggagagc tgaaaatccg tggtgttaat
     1861 gcgctgagga ttttcaacga agcttttggc ctgatttttc gtcgttcgga agagtgcctg
     1921 caccttattc ccaccagtga aggtcagggg gaaaatggcg atattggtcc cctgcgcccg
     1981 ttcaccatta atttgcggac gggtgaaata tccatgtcgc ataaagtgtc tgttggcggt
     2041 ggttcgcagg tcaatggtgc gctgggtatc ggcgttcaga acgccctggg ggggaattca
     2101 attgttcttg gtgataatga caccggattt aaacagaatg gagacggtat tctggatgtt
     2161 tatgctaata gtcagcgtgt att
//
LOCUS       DAZSIO010000322         2176 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZSIO010000322 DAZSIO010000000
VERSION     DAZSIO010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.327"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            82..390
                     /locus_tag="ACHX5T_004200"
                     /pseudo
     CDS             82..390
                     /locus_tag="ACHX5T_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
     gene            362..>2176
                     /locus_tag="ACHX5T_004201"
     CDS             362..>2176
                     /locus_tag="ACHX5T_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR8469138.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIESSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQR"
BASE COUNT          563 a          476 c          604 g          533 t
ORIGIN      
        1 gcagccttca gggaaaaata tcatcagatg gctgcttaaa aaaagaacaa atggtagtgt
       61 ccgctattgc cagtacacct caaactactc attgccagat gggaactgac cgcagaacag
      121 gctgtcgcac agcagttaaa aaatcaggtg tcaaaaggaa atcttatcga taccggattt
      181 tgtatttttg cccttagtaa gctggcgatg gctttatcca gtacgcttga ctccattcct
      241 ttgtccatgc aacgacagtt cccggattta acgccgcgtc atattgatca tctgaaaatc
      301 cttattgcta agggagcgaa tcagtgcgcg cgtgcggggg ataaattacc ggatttactc
      361 gatgaatata tcagaacaac aactgagtaa tatgatggtg gcagtcacta tcgcactgca
      421 gccactggtc agggtattgc cggtgacggc tgtagaatgg gccgatcaaa attattacct
      481 gcctaaagaa tcgtcatacg gtgaggggga atggaaaacg ctgcagtttc aggtcgccat
      541 catgaactgc atgggcaatg acgagattcg tacagttaac ctgattaaat ctgcccgtgt
      601 tggttataca aaaatggtgc ttggtgtgat cgggtatttt attgagcaca aatcccgcaa
      661 cactcttctt tttcagccga cggactcagc tgctgaagat tttatgaagt ctcacgtgga
      721 agcaacaatt cgtgatgttc cttgcctgaa aaaactttct ccctggctgg gccggaaaca
      781 ccgggataat acgctcacac tgaagcgttt ttcctctggt gtggggttct ggtgtttggg
      841 tggtgctgct gccaaaaact accgtgaaaa atctgtcgat gtggtctgct atgacgaact
      901 ctcctcgttt gaaccggatg tggaaaaaga aggttcgcca acactgcttg gcgataaacg
      961 tattgaaagt tcggtatggc caaaatccat acgcggttca acgccaaaaa ttaaaggctc
     1021 ctgtcagatt gaaaaagctg cgaatgaatc tgcgcatttc atgcggtttt atgtcccttg
     1081 ccctcattgc ggggaggagc agtatctgaa gtttggcgat gatgcgacgt cgtttggcct
     1141 gaaatgggag aagggtaaac cggaaacggt gtattacctg tgtgaacata atggctgcgt
     1201 gatccgtcag tctgaacttg atcagaccgg ggggcgctgg atttgtgaca ataccgggat
     1261 gtggacgtgt gacggtctga cgtttttcag tgcttcgggt aatgaaattc cgccgccgcg
     1321 ctccatcact ttccatatat ggacggcgta cagtccgttc accaccaggg tacagatagt
     1381 ctgtgactgg ctggatgcac tgaaagatcc caacggcgtg aaaacttttg tgaacaccac
     1441 gctgggcgag acctgggaag aggccgtggg cgaaaaactc gatcaccagg tactgatgga
     1501 taaggttgtg ccttacacgg caacggtgcc tgtccgggtg gtttatctga cggcgggcat
     1561 tgactcgcag cgaaaccgtt ttgagatgta tgtctggggg tgggctccgg gagaggaagc
     1621 ctttctggtg gataaaatca tcatcatggg acgtcctgat gaggaagaga cgctgttacg
     1681 tgtggatgcg gcgatcaata aaaaataccg ccatgcagac ggtaccgaaa tgaccatttc
     1741 ccgtgtctgc tgggatatcg gggggatcga tggcgaaatc gtgtatcaga ggtcaaaaaa
     1801 acacggtatt ttccgggtac tgccggtaaa aggtgcctct gtctatggca agccggtgat
     1861 caccatgccg aaaacccgca atcagcgggg tgtgtatctg tgtgaggtgg ggacggatac
     1921 cgcaaaagaa attctctatg cccgaatgaa ggccgatccc acccctgtga atgaggccac
     1981 atcgtatgcc atccgctttc ctgataatcc ggagattttt tcgcagacag aggcacaaca
     2041 actggtggcg gaggaactgg tggaaaagtg ggaaaaagga aagatgcgtc tgttgtggga
     2101 taacaaaaag cggcgtaacg aagcgctgga ctgcctggtg tatgcctatg cggcattacg
     2161 tgtgtccgtg caacgc
//
LOCUS       DAZSIO010000323         2164 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZSIO010000323 DAZSIO010000000
VERSION     DAZSIO010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.328"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..79
                     /locus_tag="ACHX5T_004202"
     CDS             <1..79
                     /locus_tag="ACHX5T_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469139.1"
                     /translation="SMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            93..473
                     /locus_tag="ACHX5T_004203"
     CDS             93..473
                     /locus_tag="ACHX5T_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFR8469140.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(514..1464)
                     /gene="corA"
                     /locus_tag="ACHX5T_004204"
     CDS             complement(514..1464)
                     /gene="corA"
                     /locus_tag="ACHX5T_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR8469141.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1616..1672)
                     /gene="ysgD"
                     /locus_tag="ACHX5T_004205"
     CDS             complement(1616..1672)
                     /gene="ysgD"
                     /locus_tag="ACHX5T_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR8469142.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            1834..>2164
                     /locus_tag="ACHX5T_004206"
     CDS             1834..>2164
                     /locus_tag="ACHX5T_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469143.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          583 a          507 c          514 g          560 t
ORIGIN      
        1 ttcgatgatt tttttgattt gttatttttc cactttcagt atgctaatgc ttgcacctac
       61 tgattcatca aaacaatagt tatggagtat ttatgagtaa ggaatatatg aacgatgatt
      121 cattaagtga aaaatggaaa taccgattta atttttatga tcaacatggc tttcctggat
      181 tctggggagc aacaccagaa tataaagcag catttaaagc attaaaagta agacaacgat
      241 taactattca aatgaatttc attgctttct tctgttcctg gatctattta tttgttctgg
      301 gcttatggaa aaaagcaatt atagttctat tacttggcat tctatctcta tttgttggtg
      361 cattaattgg cgttaacatt ctgggaattg ccgtagcagc ctatgttgct gttaacacga
      421 acaaatggtt ttatgagaaa gaggtaaaag ggttaaatac ctggagcctt tgaataacag
      481 gtggcttagc caggctaagc caccgccttc tttttacaac cagttcttcc gcttaaagta
      541 cagatacggt gccaggcccg cgaggatcat aaagataatc gcgccagggt agccgaaact
      601 ccacttcagt tctggcataa actcaaagtt catcccgtag ctggaagcaa ccagcgttgg
      661 cggcaggaac acgacggata ccaccgagaa aattttgatg atgcggttct gctcgatgtt
      721 gataaaaccc attgccgcct gcatcaggaa gttcaccttc tggaacaggg attcgttatg
      781 cggcagcagg gattcgatat cgcgcaggat ttcacgcgcc tgctccagtt gcccacccgg
      841 taaacgcgct ttacgcacca ggaagttgag agcgcgctgg gtatccatca gacacaggcg
      901 aactttccag ccgatatctt ccagttccgc cagcgtggag agcgcctcgt cgtactcatc
      961 gccctgatgc ccttccataa tcacccggct caactgctcc aggtcgctat agatattttc
     1021 aatttcatct gccaactgtt cgattttggt ttcgaacaga tccagcagca actcgtaggc
     1081 gttaccgtct accatcgact ggctacgggc acgcatacga tacagacgaa aagcgggcag
     1141 ttcacgctca cgcagagtaa acagacgacc atcacggatg gtaaatgcca cagtggagtt
     1201 accggcgtga tcttccgcat cttcaaagaa gaagaaggag tgaatatgca ggccgtcgtc
     1261 gtcttcaaag aaacgtgccg atgcttcgat gtcttccagt tccgggcggg ttgccaggct
     1321 ctggccaagt tcagattgta cgcgcagtcg ctcgtcgtcg tccggttcga caagatcaat
     1381 ccatactgca tttacaaggg gttgtgactc ttcgacttcc agccgggtca gtcggttatt
     1441 ttccagttga aatgcgctca gcatgaccgg gactcccaat gcttaaaata tcggacagtt
     1501 cggtgggcac acagaaacaa attgggtttc agaccattaa acagcctgac tcagcgcgac
     1561 gggaaaaatt gaggtcgctg acaaccgcta aggctatcag caaaaaggga tagccttagg
     1621 agttgatcct ggatgacagg atagtgagcc agtatctact gggtgtgtcc aaggcgaatg
     1681 tcctcttaga gtgatcgtgc gcgcatgtta cgccagcaaa attttgccgt caacacgcaa
     1741 cagaacacca cagaacaata gttgaacttc gcgctttttg gtactcgtca aggtcgctaa
     1801 caggataaaa aaatcccaca tctaggacct ataatggcgc atcggctact cattggtaaa
     1861 ggaatgatca ccttgaatct caaacggatt tttctcaccc tcaccttact tcccttgttt
     1921 gctgttgccg ctgatgattg cgcactctca gatccgacgc tgaccgtaca ggcgtatacc
     1981 gttaatcctc aaacagagcg ggtgaaaatg tactggcaaa aagccaatgg cgaagcgtgg
     2041 ggaacgttac atgctctgct ggcggatatt aatagtcagg gtcaggtgca gatggcgatg
     2101 aacggcggca tctatgatga aagctatgcg ccgctcggtt tgtacatcga aaacggtcag
     2161 caga
//
LOCUS       DAZSIO010000324         2143 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZSIO010000324 DAZSIO010000000
VERSION     DAZSIO010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.329"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..357
                     /locus_tag="ACHX5T_004207"
     CDS             <1..357
                     /locus_tag="ACHX5T_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR8469144.1"
                     /translation="VFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
     gene            619..981
                     /locus_tag="ACHX5T_004208"
     CDS             619..981
                     /locus_tag="ACHX5T_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFR8469145.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            978..1907
                     /locus_tag="ACHX5T_004209"
     CDS             978..1907
                     /locus_tag="ACHX5T_004209"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR8469146.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            1904..>2143
                     /locus_tag="ACHX5T_004210"
     CDS             1904..>2143
                     /locus_tag="ACHX5T_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HFR8469147.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSF"
BASE COUNT          605 a          434 c          476 g          628 t
ORIGIN      
        1 gtgtttgtcc acaccaaacc tgcctaccga agcgacggaa caaaaacagc agcggtaagg
       61 aagatgagaa ccgacagcaa caaggcatgg aagggagcgt taaagcgggc aggcattagc
      121 aacttccgct tccatgacct gaggcatacc tgggcaagct ggctggttca gtccggtgtc
      181 tctcttcttg cgcttaaaga gatgggagga tgggaaactc tcgaaatggt tcaaagatac
      241 gcccaccttt cagccgggca tcttaccgag cacgcgagca aaatcgatgc gattataagt
      301 cgcaatggca caaatacggc acaagaggag aacgtagttt acttaaatgc gaggtaactt
      361 attgatttaa atggtgccga taataggagt cgaacctacg accttcgcat tacgaattat
      421 aagaatccgc ttgtaattca aaggattatc ccatcaacac tgcgctcaca cgtcccacca
      481 catcaaaaca tgtaaagcct tgcaagccat tgtgaggcct tatgtgtctc agttttgtct
      541 catcagacat agcaagtatc gatcaattga gacttggatg atagacttca tgcctttcag
      601 agctcattga ttaaataaat gttaaagtta tttgtaaagt acacatctat aggtgtgctg
      661 aacacactta tacactgggt ggtttttggt gtatgtatct atgccgcaca taccagtcag
      721 gctctggcaa acttcacagg tttcgtagtg gctgtgagct ttagcttctt cgcgaatgca
      781 agattcacat tcaaagcatt gactacagcg atgcgctaca tgttatacgt gggatttatg
      841 ggaatattga gtgtgattgt tggttgggca gctgataaat gctcacttcc tccaatagtc
      901 actcttatca ccttctccgc catcagtctg gtgtgcggtt tcgtctattc aaagttcatt
      961 gtctttaggg atgcgaaatg aaaatatctc ttgtcgttcc tgtcttcaat gaagaagaag
     1021 cgatacctgt tttctataaa acggtacgtg aattccaaga gttgaagcca tatgaagtag
     1081 aaattgtatt cataaatgac ggaagtaaag atgccacaga gtcaattatt aacgcgctgg
     1141 ctgtttcaga cccgctagtt gttccgctgt catttacacg caactttggt aaagaaccag
     1201 ccttatttgc agggttagac catgcaagcg gcgatgctgt aattcctatt gatgtcgacc
     1261 tgcaagaccc aattgaggtt atccctcatc ttattgaaaa gtggcaggca ggtgctgaca
     1321 tggtgcttgc taaacgttca gaccgctcaa ctgatggacg actgaaacgt aagacagctg
     1381 agtggttcta taaattacac aacaaaataa gcaccccaaa gatcgaggaa aatgtcggag
     1441 attttcgact catgtctcgt gaggttgtgg agaacattaa actgttgcct gagcgcaatc
     1501 ttttcatgaa aggcatactg agctgggtgg gtggtccgac ggatgtcgtt gaatatgtac
     1561 gcgcagagcg tgttgctggc atctcaaaat ttaatggctg gaaattatgg aatctggcac
     1621 tggaaggtat cacaagtttt tcaacctttc ctcttcgcgt atggacttat ataggcttgt
     1681 ttgttgcaag catttcattt ttatatggtg catggatgat tatagacacc cttgtctttg
     1741 gtaacccagt acgcgggtat ccctccctgc ttgtatcaat acttttcttg ggtggagtgc
     1801 aactgatcgg gattggtgtt ctcggagaat atataggtag aatctatatt gaagtaaaaa
     1861 atagacccaa atacatcata aaaaaatctc atcgaggtaa cccatgatta aaattaattt
     1921 atttaaaaat gcaaatctcc ttgccttcat atcatgtttt gcaatatcaa tttattgtta
     1981 ttgggggtgg ttgtacgatg gaacacttaa tattgatggc gagtttacaa ataattttta
     2041 tcaaacaatc acgcttgggc ggtggtttca cacttttttg cgacattact ttctccctga
     2101 gcctttttca ctttatataa caccattaat agccttatct ttt
//
LOCUS       DAZSIO010000325         2133 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZSIO010000325 DAZSIO010000000
VERSION     DAZSIO010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.330"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..103)
                     /locus_tag="ACHX5T_004211"
     CDS             complement(<1..103)
                     /locus_tag="ACHX5T_004211"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HFR8469148.1"
                     /translation="MCHPQPKPPAPPAWAMMPPSNSLQLLDETFSVSG"
     gene            complement(66..398)
                     /locus_tag="ACHX5T_004212"
                     /pseudo
     CDS             complement(66..398)
                     /locus_tag="ACHX5T_004212"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            complement(395..928)
                     /locus_tag="ACHX5T_004213"
     CDS             complement(395..928)
                     /locus_tag="ACHX5T_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR8469149.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(994..1623)
                     /locus_tag="ACHX5T_004214"
     CDS             complement(994..1623)
                     /locus_tag="ACHX5T_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469150.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1627..1842)
                     /locus_tag="ACHX5T_004215"
     CDS             complement(1627..1842)
                     /locus_tag="ACHX5T_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR8469151.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          546 a          567 c          469 g          551 t
ORIGIN      
        1 tcccggatac cgagaacgtt tcgtccagca actggagaga gttcgacggt ggcatcattg
       61 cccatgccgg aggcgctgga ggtttcggct gaggatggca cagggcattt tcctttgacg
      121 agcacccgac caccattatc aagcttgcgc cgaagagcat cattttcagc tttcgcatca
      181 gctaactcct tcgtgtattt agcatcgagt gcatcagcat cacgctggcg ctgctgcatg
      241 tcagtaatgg ttgcgttcgc cagcttcagt tctccggcgt ttttgtcgcg ctgctctttg
      301 taggtaatgg cgttatcacg gtaatgatta acagcccatg acaggcagac gatgatgcag
      361 ataaccagag cggagataat cgcggtgact ctgctcattg atctatcccc caacaggcta
      421 atgcgctttc ctggtcacga cgaataacct gtccatagca gttatttgaa cgtatgcggc
      481 aatcgcgccc aacatctttt atccaccagc gaatcgcctc gcatgcgccc ttacgatcac
      541 cggcattcag ccgcttataa aacgtcgacg ggaaacactt accggggcca atgttatagg
      601 ggcaaaatga cgctataccc gctttctggg gttcggtcag aggtacttta atattgcgct
      661 ccacccatgc cagcgcctta tcacgttcaa tagcgttaac ctggtcacat ttttccttcg
      721 acagcttcat tcccggtatg acaggcttac catccaccat tgtggcacca cgacagatgg
      781 tccatatgcc agaaccatca cggtatgccg ttgtgtgatt accctctttt tcgttcagaa
      841 actggtcaag tatctgggga gcagacgctc ctgcggcaat cagcgccaga acagcagccg
      901 acaggccgta tttgattttt gcgctcatgg atatttatca ggatttatcg gcaacaaata
      961 acgagccaac ttatatacgc cttttaagat aagtcagccc cggctggatc cagtcatcag
     1021 gctctttctt aaagggggag tattgaaatc acgaagaaga gcctcccgca ccgcagcatc
     1081 catgtctgca ccactggcaa gtgcttcaat ctctgccgcc acctgcaaat accccatgca
     1141 acggccaatg cgtttcataa ctctggtgtt ttcgtctttc aggcatctga tagcgagatt
     1201 aataattttc aggcagtgat gatgcgatgc ccggacaggt tttccgttct cataaacgac
     1261 gaactcaccc tcttcacagg aaaccctgaa aggccgatgg ggttcctggt ctttatccgg
     1321 tgcagacgca ataaaatcac ttttatgctc ttcctctccg gcactgttaa ccgtaatgac
     1381 gtactcaggc cgcaggtaag ccgcctcttt ttctgacaat gcggccggta aaaacttttc
     1441 ctgcaaaaac ttcataaaag tctcgtgagc gactttgtgg cagtaatcgt aaatcgccgc
     1501 ataatgctca tcgcggcgtt tgttttcatc ttcagaactc accaacgccg acaatttttt
     1561 attaagctca gtgatttcat tttccaggtg attgaatcgc tgattcattt cttcatgctt
     1621 cattatttac tctccccgtg cagccttacg cttgtcttct cttattttga aatacagatt
     1681 cgtcagataa gtcagaagcc ccagaagcag acttcccagc acaccaatcg cagcccactg
     1741 tgatggcctg acctgatcca accactgcaa aaaccagtag ccggcactgc cggcggaggt
     1801 gccgtaggca atgcccgttg aaattttgtc catggatttc atagcctcac ctccgcaaat
     1861 aacggatggc gtagttttac actgagaaat gaaagggatt tgaaaagaaa aaaacgcaaa
     1921 agcgggcgaa acgatatata cagtaaggaa agcactctat ccaacaaacc acccacagtt
     1981 aatcggaata aaagcagagt gcttatgaat gatcgcacgc ccgaaggtta gtatttctgc
     2041 acagcaattt tgcaaaaaaa ggcgatcatt cataacttaa acgtctttca gtcactccgg
     2101 gatttcccat catcgcagac tgaaagactc tga
//
LOCUS       DAZSIO010000326         2123 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZSIO010000326 DAZSIO010000000
VERSION     DAZSIO010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.332"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..1065
                     /gene="ompC"
                     /locus_tag="ACHX5T_004216"
     CDS             <1..1065
                     /gene="ompC"
                     /locus_tag="ACHX5T_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR8469152.1"
                     /translation="AISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKHYFSSND
                     ADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFAGLKFG
                     DYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNNDFFGLV
                     DGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQVIYGNN
                     SLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEVVAQYQF
                     DFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINLIDKSDF
                     TKASGVATDDIVAVGLVYQF"
     gene            complement(1554..2123)
                     /locus_tag="ACHX5T_004217"
                     /pseudo
     CDS             complement(1554..2123)
                     /locus_tag="ACHX5T_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          611 a          443 c          431 g          638 t
ORIGIN      
        1 gcaatttctg ctgtagctgc atcagtactg atggcgatgt ctgctcaggc agcagagatt
       61 tacaataaag acagtaacaa gctggatctg tacggaaaag ttaatgctaa gcactacttc
      121 tcttctaacg atgcagatga tggcgacaca acttatgttc gtctgggctt caaaggcgaa
      181 actcaaatca acgatcaact gactggtttc ggtcagtggg aatatgaatt caaaggcaac
      241 cgtgctgaat ctcaaggttc ttccaaagat aaaacccgtc ttgccttcgc tggcctgaaa
      301 ttcggtgact acggctccat cgattatggc cgtaactacg gtgtagcata cgatatcggt
      361 gcatggaccg acgttctgcc agaattcggt ggcgatacct ggacccaaac agatgtgttc
      421 atgactggtc gcaccacagg tgttgcaacc tatcgtaaca acgacttctt tggtctggtt
      481 gatggtctga actttgctgc tcagtatcag ggtaaaaatg accgcactga cgtaactgaa
      541 gctaacggtg atggtttcgg tttctccact acttatgagt atgaaggatt cggtgtaggt
      601 gcaacctatg ctaaatctga ccgcactaat gatcaggtta tctacggtaa caacagcctg
      661 aatgcatctg gtcaaaatgc tgaagtatgg gcagctggtc tgaaatatga tgcgaacaac
      721 atctatctgg ctaccaccta ttctgaaacc cagaacatga ctgtttttgg taataaccat
      781 attgccaaca aagcacaaaa cttcgaagta gttgcacaat atcagttcga cttcggtctg
      841 cgtccgtccg ttgcttacct gcaatctaaa ggaaaagact tgggtgcgtg gggtgatcag
      901 gacctgattg aatatattga tgtaggtgca acctattact tcaacaaaaa tatgtccact
      961 tttgttgatt acaaaatcaa cctgattgat aagagcgatt tcacgaaagc atctggcgtt
     1021 gctaccgatg atatcgttgc tgtaggattg gtttaccagt tctaatctga ttactcaaag
     1081 atatgttgtg ggaggctttg cctccccaac atataagtgg cgctctcaag ccactttcct
     1141 tagaagcact atcttgcttc tgactatata aattaccctt tattttctgg gacgtttcaa
     1201 cgccccattt ttaataatac tcagtgaaca attaacgtat caattagatt taataacaac
     1261 gatatccgta cctaacctga tatctaatgt cataagcatc cctttaatta tcccctcaac
     1321 ctcttgtaaa tttcccactc ttgagctcca catagcccaa acgccggatg caaaaaaaat
     1381 catacgtgca tctctgaaat tgtgatggag gaagtgagta tcaattatat gtgcttataa
     1441 acgttcccga ttggcacaga agtcaccgga ttgttcaggc tccaataggt tgagtatgtc
     1501 gtatttattt tccaaaataa aaatcgatgc tgttgctcct taataaaata cttttactct
     1561 ctggacgctt ttcttagctc tcttggtgtt atattaaaac gattatggaa cctttcagta
     1621 aaacgagaag gacacttgta accattttcc ctggcaatct cgctgatggg ttttactgtc
     1681 gtttgtatag caggacaacg cattatttaa cctcacgtcg tccaatatac tttgaaaact
     1741 tattccctcg cttgccagac ggcgatgcaa tgtggaaaca gaaatgtaga gatatctcgc
     1801 taccttgttt gctgtccatt ttgttctagg ttcggatagc attaggttat aacaacgact
     1861 tattaatgat tgtttactat ctgataaaag taaacaatta acatgatgtg ctcctaatga
     1921 aagtagaacg cccattgcta agtgttcctg aatttgcgtt gagaagcctc gggaaataga
     1981 tgtttttagt tgctcccaac aatatattaa ctcggggttc tgaggtaagc agaaacctgt
     2041 cgtgttacga atttgatcag ttatcgcata aaatttttga aacttttcaa ttagatcaat
     2101 gggataataa agcatttctg caa
//
LOCUS       DAZSIO010000327         2092 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZSIO010000327 DAZSIO010000000
VERSION     DAZSIO010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.334"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="ACHX5T_004218"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="ACHX5T_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR8469153.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /gene="mcbR"
                     /locus_tag="ACHX5T_004219"
     CDS             1351..2016
                     /gene="mcbR"
                     /locus_tag="ACHX5T_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR8469154.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          444 c          559 g          512 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgcccatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tt
//
LOCUS       DAZSIO010000328         2090 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZSIO010000328 DAZSIO010000000
VERSION     DAZSIO010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.335"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..797
                     /locus_tag="ACHX5T_004220"
                     /pseudo
     CDS             1..797
                     /locus_tag="ACHX5T_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            912..1253
                     /locus_tag="ACHX5T_004221"
                     /pseudo
     CDS             912..1253
                     /locus_tag="ACHX5T_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1236..2073
                     /locus_tag="ACHX5T_004222"
                     /pseudo
     CDS             1236..2073
                     /locus_tag="ACHX5T_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          516 a          505 c          632 g          437 t
ORIGIN      
        1 tgggccttgc ccgtagcagt tatttttatc accgggctgc actgaaagcc ggtgataaat
       61 acgccacgat acgtaagatg ctgacagata tctttaacag taattaccag tgttatggct
      121 atcgtcgcct gcatgcgatg ctcaggcatg agggggggcg gctatcagaa aaggttgtac
      181 gcagacttat ggtggaggaa cagcttgtcg tcagtcgtaa ccgtcgtcgt cgctacagct
      241 catattgcgg agaaatcgga ccggctccgg ataaccttat cgccagagat tttaaggcgg
      301 agcaacctaa tcagaaatag ctgacagata tcacggagtt ccagctccct gcaggtaaag
      361 tctggctgtc accagtggtg gactgcttcg atggaaaagt tgtgagctgg tctctcagta
      421 cacgccctga tgctgaactg gtcaacacta tgctggatag cgctgtcgaa acgttaaatg
      481 ctggcgaacg accggtgata cacagtgaca gaggtgggca ttatcgctgg ccaggctggc
      541 tggaaagagt gaatgcagca ggtcttattc gctcaatgtc ccgtaaagga tgttcacctg
      601 ataatgctgc atgcgaaggc tttttcggca gactgaaaac ggaaatgtat tatgggcgta
      661 aatggtcggg catcacgcca gaaaagttca tgcagcaagt ggatgcttac atcagatggt
      721 ataacgagcg gcgtataaaa ttatcgctgg gtgccgtcag ccccaaaatg taccgccaac
      781 aatgcgggct ggaatgataa agcagtccag gaaatcgtcc gcatcccccc ttcaccattt
      841 ggattgtccg ctccacccaa catgttgttt ccttaaggtt cctacaccac aaaggaaact
      901 ctcatgctgc gcagtggatg gctgacgttg ccgacaggcg ggaacttcgc cagttcaggc
      961 agacaccgga acagcgcttc acgcaggaac aggagcatct gcagccgctg ctgggtacgg
     1021 acttcgatat ccgtcatgtc tcctgggatg gctatatcga ggttggcggg aaccgttaca
     1081 gtgtgccgga aagcctgtgt ggtcagctgg tatcaatacg gatctcgctg gatgatgaac
     1141 tgcggatcta cagtaatgag caacaggtag catcgcaccg tctctgttcg gctgcatacg
     1201 gctggcagac cgtgccggag catcacgctc cactctggca gcaggcttcg gagcgcatgg
     1261 ccgaatggct gggggagata cagaaacggg taatcactgt ctgtgaccgg gaagcggata
     1321 tctggcatta cctgtattat aaagtcagcc acggacagcg tggtgcgtgc tgcacagaat
     1381 cgccggctgg aagaggcacc cggcaagctc ttcgaactgc cggaagtcct ggcaaccgca
     1441 ggaagccaca cgctgaatgt gatgcaaaaa ggcgggcggg cagcccgtca ggcccggatg
     1501 ttcatcagtt acagcgaagt cagcataaaa aatcccgaca acagcggcca ggcgctcccg
     1561 ctcacgtatg tctgttgccg ggagcaggca gaggacggtg cctgctggca tctgctgacg
     1621 tcaggaaaag cggcgagtgc cgccgatgca cgacgtattg tcagccatta cgagcgacgc
     1681 tggctgacag aggaatacca caaggcgtgg aaaagtggtg gtacatggaa tcgctgcgaa
     1741 tgcagacccg ggataacctt gagcgcatgg tggttatcct ggcgtttata gcggtacggg
     1801 tgctgggcct gcggcaggga ggtgtcagtg aagaaacaca aaatgacagc tgtgagaaaa
     1861 tactgacgcc gacagagtgg aaactactgt gggttaagct ggaaggaaag ccgcttccgg
     1921 tgcaggcacc gaccctgaaa tgggcctggg gcgatggcat gacagcaaac gcacaggtcg
     1981 tcccggttgg ggcgtcatgt gggatggctg gtcagacttc aggatatggt tgagggttac
     2041 ctggtgatga agtctcttga tcaggagctc tgatcaagag acagcagctt
//
LOCUS       DAZSIO010000329         2076 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZSIO010000329 DAZSIO010000000
VERSION     DAZSIO010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.336"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            156..1133
                     /gene="intI2"
                     /locus_tag="ACHX5T_004223"
                     /pseudo
     CDS             156..1133
                     /gene="intI2"
                     /locus_tag="ACHX5T_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            complement(1321..1560)
                     /locus_tag="ACHX5T_004224"
     CDS             complement(1321..1560)
                     /locus_tag="ACHX5T_004224"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR8469155.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT          603 a          451 c          434 g          588 t
ORIGIN      
        1 ctcacccaaa gaactcataa cattttaatt tatttagcat taaattaaaa ctctaccggt
       61 aaacaagcat ctctaggcgt aaaaaacagg cactttttta aatcccagct agaataggct
      121 gtataggcag acagttgcaa gacaaggagc gttttatgtc taacagtcca tttttaaatt
      181 ctatacgcac ggatatgcga caaaaaggtt atgcgctgaa aactgaaaaa acttacctgc
      241 actggattaa gcgttttatt ctgtttcaca aaaaacgtca tcctcagacc atgggcagtg
      301 aagaggtcag gctgttttta tccagcttag caaacagcag acatgtagcc ataaacacgc
      361 agaaaatcgc tttaaatgcc ctagcttttt tgtacaacag gtttttacaa cagccgttgg
      421 gcgatattga ttatatccct gcaagcaagc ctagacggct accctctgtt atctctgcaa
      481 atgaagtgca acgcattttg caggttatgg atactcgcaa ccaagttatt tttacgctgc
      541 tgtatggtgc aggtttgcgc attaatgaat gcttgcgttt gcgggttaaa gattttgatt
      601 ttgataatgg ctgcatcact gtgcatgacg gtaagggtgg gaaaagcaga aacagcctac
      661 tgcccacgcg cctaatccca gcaataaaat aactcattga gcaagcgcgg cttattcagc
      721 aagacgacaa cttacaaggc gtagggccat cgctgccttt tgctttagat cacaaatacc
      781 cttctgctta tcgacaagcg gcgtggatgt ttgtctttcc ctccagcacg ctctgcaacc
      841 acccgtataa cggcaaatta tgccgccatc atctgcatga ctccgttgcg cgaaaggcat
      901 tgaaggcagc cgtacaaaaa gcaggcatcg ttagcaagcg tgtcacttgt catacatttc
      961 gtcactcgtt tgctacgcat ctattacaag cggggcgtga tattcgcact gtgcaagaac
     1021 tcttagggca taacgatgtt aagaccacgc aaatctatac gcatgtgttg ggtcagcatt
     1081 ttgccggcac caccagtcct gcggatggac tgatgctact tatcaatcag taaaagtaga
     1141 taaatatctt tatgtagccc tgagtagccc ctgatacttt ttatggtgaa aaagataaac
     1201 aatcatagag gggataaata tgagccagtt ctaccattgt tcagataagt ttaagcaaga
     1261 tactgtcaat cacagtgacc attcatggtc attacacttc ctccagttgc cagacaatgc
     1321 ctaacataaa aacgcaaaag cccaaaagtc agagcttagg tgttctaggc gttttctaag
     1381 cgctgaagaa atacgtctta ccgtagattg cgtgcgtttc tgaagcacat tttccttata
     1441 catggcttta tcccaggcag cttcatcaag ctcttggagt aacaagccag taataatgcg
     1501 acactctcgg gtttgtagcg agccgctggt gagatcggag ttatagcgga aaggggtcat
     1561 ttgtaattat ttgtgggtgg aggatagtta acttattatt gtagttggaa gaggctgctt
     1621 tccaaacatt agcacctcgt aatgcttgtt actgtatatt aatttatgct cttagcgcat
     1681 gttaatgggc tattacaatg cataatgtcc acatcaagat aactgtggct taacacctgt
     1741 acaagcgaaa aaaatcaatc acctgtccgg aaatacggga tcactgtaaa gtgttggtga
     1801 ggcagtctag gagggggatt taatttggga tactgatgaa aggattatga tcagttggat
     1861 ataggatcgt actaagctga gcggcaggtt agctagatta gttaaccaga taagtgaaat
     1921 ctagttccaa actattttgt catttttaat tttcgtatta gcttacgacg ctacacccag
     1981 ttcccatcta ttttgtcact cttccctaaa taatccttaa aaactccatt tccacccctc
     2041 ccagttccca actattttgt ccgcccacag ctgccg
//
LOCUS       DAZSIO010000330         2049 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZSIO010000330 DAZSIO010000000
VERSION     DAZSIO010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.337"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            9..749
                     /gene="phoN2"
                     /locus_tag="ACHX5T_004225"
     CDS             9..749
                     /gene="phoN2"
                     /locus_tag="ACHX5T_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HFR8469156.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            822..944
                     /locus_tag="ACHX5T_004226"
     CDS             822..944
                     /locus_tag="ACHX5T_004226"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469157.1"
                     /translation="MGTPRFTPEFKGLLERLGHIPPAEAEKAYYAAIGNDDLAT"
     gene            complement(1102..2049)
                     /gene="ospC4"
                     /locus_tag="ACHX5T_004227"
                     /pseudo
     CDS             complement(1102..2049)
                     /gene="ospC4"
                     /locus_tag="ACHX5T_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
BASE COUNT          627 a          409 c          366 g          647 t
ORIGIN      
        1 aataaattat gaaaaccaaa aactttcttc ttttttgtat tgctacaaat atgattttta
       61 tcccctcagc aaatgctctg aaggcagaag gttttctcac tcaacaaact tcaccagaca
      121 gtttgtcaat acttccgccg cctccggcag agaattcagt agtatttcag gctgacaaag
      181 ctcattatga attcggccgc tcgctccggg atgctaatcg tgtacgtctc gctagcgaag
      241 atgcatacta cgagaatttt ggtcttgcat tttcagatgc ttatggcatg gatatttcaa
      301 gggaaaatac cccaatctta tatcagttgt taacacaagt actacaggat agccatgatt
      361 acgccgtgcg taacgccaaa gaatattata aaagagttcg tccattcgtt atttataaag
      421 acgcaacctg tacacctgat aaagatgaga aaatggctat cactggctct tatccctctg
      481 gtcatgcatc ctttggttgg gcagtagcac tgatacttgc ggagattaat cctcaacgta
      541 aagcggaaat acttcgacgt ggatatgagt ttggagaaag tcgggtcatc tgcggtgcgc
      601 attggcaaag cgatgtagag gctgggcgtt taatgggagc atcggttgtt gcagtacttc
      661 ataatacacc tgaatttacc aaaagcctta gcgaagccaa aaaagagttt gaagaattaa
      721 atactcctac caatgaactg accccataaa gctggacagc ctgtatcagg ctatggaggg
      781 cccatagaca aatctaccct atatgagcat aggaggagtc tatgggcaca ccacgtttta
      841 cccctgaatt taagggatta ctggaaaggc tgggacatat ccctccggca gaagcagaaa
      901 aagcttatta tgctgccatc ggaaacgatg atctggcaac ctgagttcac agataaaaca
      961 ttctctagga aactcggggc ggttccgttc accacatgca atgtggtgtt gcaggggaac
     1021 ggtctgcacc atcccctatg tcgatcaata taacagaaat gacaacttca gattcagggc
     1081 acaacctaaa tatattttag gtcacctctc aaatcgtttg cctgataccg ctccattttt
     1141 taataaaaaa atcaatcatt tttgaatctt tgcttttcat tgcattgtca agcatagtgt
     1201 ccccactatt tgctttttga aattttttgt ttacatcaac caatccatta ttgataaaat
     1261 attctagcac tttatagttg gcaccatctt tactaagtaa gtattcaaca tcatacagat
     1321 tacaatatct attgtttttc attttctcca tctctgcgac atcttgtttt gtaaaattaa
     1381 atttagatag taaataaagt gctatatcag cttttttatt gcctaaagaa taagcaaggg
     1441 cctgatgagc cattttttta tcagtcactt tattgttaat ctcttcataa ttagatgctt
     1501 taatcgcatc ctccaaagat atgtctctga gttttatttt tttgaagtta tctgtactta
     1561 gcattcttgg tatatgatac tcaagctgaa acacaaagtt tataattcta tccagactaa
     1621 caggatcaat atttttgtta taacaaaact ctctgaatcc ttgatcttta ctctttctta
     1681 taaaatcaat aagatgcaac ccaattccaa gcctcatatc tttagtgtta aatattggaa
     1741 catcagtttt cccctgatta ccatgcacat acctgctaac ttcatccaca acctctttaa
     1801 agttatctaa gaaaaatgat tggtgctcat ggtaaaaaac aggtggaata gcattatcaa
     1861 agtgatcggt taatgtcata tatccatatg gagagtcatg atcaatgata tacgcattgg
     1921 cgccaaaatc tacagtatga tgtgttttat ttagtgggag tttttcttga tgattggaaa
     1981 tttcgacccc aaaaaaaaca aaatcgtggt tagagatttc tttaatatcc tcagagtatg
     2041 tattcttat
//
LOCUS       DAZSIO010000331         2049 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZSIO010000331 DAZSIO010000000
VERSION     DAZSIO010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.338"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            67..585
                     /gene="mddA"
                     /locus_tag="ACHX5T_004228"
     CDS             67..585
                     /gene="mddA"
                     /locus_tag="ACHX5T_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR8469158.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            582..1031
                     /locus_tag="ACHX5T_004229"
     CDS             582..1031
                     /locus_tag="ACHX5T_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR8469159.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1032..1265)
                     /gene="ydcY"
                     /locus_tag="ACHX5T_004230"
     CDS             complement(1032..1265)
                     /gene="ydcY"
                     /locus_tag="ACHX5T_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR8469160.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1351..1524)
                     /gene="ortT"
                     /locus_tag="ACHX5T_004231"
     CDS             complement(1351..1524)
                     /gene="ortT"
                     /locus_tag="ACHX5T_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR8469161.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1719..1814
                     /gene="yncL"
                     /locus_tag="ACHX5T_004232"
     CDS             1719..1814
                     /gene="yncL"
                     /locus_tag="ACHX5T_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR8469162.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          492 a          467 c          502 g          588 t
ORIGIN      
        1 ccgttgcttc gggctgtttt cgatagtatt gataacatca tcatcattat tttattgagg
       61 ccagacatgt ccatccgttt tgcccgcaaa gccgactgtg ctgccattgc ggaaatttat
      121 aaccacgccg tgttgtatac ggcggctatc tggaatgacc aaacggtgga tgcagataac
      181 cgcattgcct ggtttgaagc gcggaccata gcaggttatc cggtgctggt aagtgaagaa
      241 gatggcgtag ttacgggata tgcctcgttt ggcgactggc gtagtttcga tggttttcgc
      301 cataccgtgg aacattcggt ttatgtccat cccgatcatc agggcaaagg tctggggcgt
      361 aaattgttaa gtcgattgat tgatgaagcg cgggattgcg ggaagcatgt catggtcgcc
      421 gggatcgaat cgcaaaatca ggcttcgctg catctgcacc agtcgctggg atttgtcgtc
      481 accgcacaaa tgccgcaggt aggcactaaa tttggtcatt ggctggatct gacatttatg
      541 cagttgcaac tcgacgagcg cactgaaccg gacgcgattg gatgaatcag tcgctcaccc
      601 ttgctttttt gattgccgcc ggaattggtc tggtagtcca gaacacgctg atggcgcgca
      661 tcacccagac ctcctctacc attctcatcg ccatgttgct gaactcactg gtggggattg
      721 tgctctttgt ttctatttta tggtttaaac aaggcatggc ggggtttggc gaactggtgt
      781 ccagcgtgcg ctggtggaca cttatacctg gcttgctggg ttcgtttttt gtcttcgcca
      841 gtatcagcgg atatcagaat gtgggagcgg cgacaactat tgcggtactg gtcgccagcc
      901 agctgattgg cggtctggtg ctggatatct tccgcagtca tggcgtacca ctacgggcgt
      961 tgttcggccc aatatgtggc gcaattttac ttgtggttgg cgcctggttg gtggccagac
     1021 gctcatttta atcagaggat ggtgccgcct ttggttaact gttcgtggcg cgcttccata
     1081 tcttctttat gcttgcgacc gtgatgagca atagcggtgc gcaaacgctg ctgctgggtg
     1141 tagcgatctt cccgacttaa ctccgcgtca tcgctcaggg cgattaataa ttcgttcata
     1201 tgggcaatga cgctctcttc aatcgcggca tccacgcgcg cgatgacttc atccagatgc
     1261 gacatacttt ctccttagcc acttgtgaaa tttatcgatt aatccaaatg atataagtct
     1321 gcgtggtgcc gtcaagccag tgagtaagat ttaaaacagt gaaaacaaca gcgccaccgg
     1381 gaaactcatc ggccatgtgg cccccaccag aaaggcgctt aagaaacgga tgcgtttctt
     1441 atcgtgagaa agaaaccagg tgataagaaa tgcgattgct gccataaccg cataaaaaac
     1501 aagcatatgt tgatagagaa acatgtacgg gcgtccagcc atatgaataa aacgcgcgca
     1561 aatatgcgcc attttttgac gtgcatcaag ttttttcacc agtttgagaa aaaatcatca
     1621 aacagcaact tgcccttatc catcttcaga atgagtatta ttgcggattt tcttaacccg
     1681 tactatacac ataggggcaa tgataaaagg tggcaaaaat gaatgtttcc agtagaactg
     1741 tagtactgat aaatgtcttt gctgctgttg gtttgtttac tcttatctct atgagatttg
     1801 gctggtttat ttgatgtagt gattttgcac gcagcacgta ctgtcagaga aatcaactct
     1861 ctgacagtac gcaagatctt ctcatactct ccaagtttgc aacattatcc ttaatattta
     1921 cttctttttg ctggcatcta cattgaaatg agtagaaaac atccccctac tgtcgtctat
     1981 acaaacagct tactgattca aacggaatct tgttttcaca tttttctatc acgaatactt
     2041 tatacgcgc
//
LOCUS       DAZSIO010000332         2040 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZSIO010000332 DAZSIO010000000
VERSION     DAZSIO010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.339"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(83..817)
                     /locus_tag="ACHX5T_004233"
     CDS             complement(83..817)
                     /locus_tag="ACHX5T_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8469163.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            complement(817..1971)
                     /locus_tag="ACHX5T_004234"
     CDS             complement(817..1971)
                     /locus_tag="ACHX5T_004234"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR8469164.1"
                     /translation="MYLALTARTRSLTLICLLSLYPLSTALVLTQSRVGLLGLFCVIV
                     LAALADRPRWRGRPLSGLLRLIFVASLLCWCVGISNYAMPGETTADFTHSSSNDQRIR
                     ILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGVTNIESDTATHPHNELLFVTAEGG
                     IVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEKWFIALMGLPLVIHMMTEYPLYTS
                     VPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILPVIVLIMTAALVILVAGFDAQREL
                     TRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHLLIANKPGFVRDTESMSLFTVWGN
                     KWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRAARVFVNDERFTRGGN"
     gene            complement(1972..2028)
                     /locus_tag="ACHX5T_004235"
                     /pseudo
     CDS             complement(1972..2028)
                     /locus_tag="ACHX5T_004235"
                     /inference="COORDINATES: protein motif:HMM:NF027197.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          571 a          527 c          442 g          500 t
ORIGIN      
        1 atagcaccca tctattacta acctcaccac gcatgctcag cgatttcgtc atcgaaaaag
       61 agagttatct atgacgcatc aattatactt tttgctttgt tcgcatattg ttacatcttt
      121 cataccttga cgcatctctt cccaacgcgg gtatcgcctg ttgtataagt tgagcattac
      181 gtactaacca gacgtactca cttttattcc tggtgcctaa tcgttctcga attgcggact
      241 catgggcgct aacggtcttg aggccaatac cacagatttc tgcaatttta cgtgtgctaa
      301 tccccttcgc gcggagtaaa agaaccctcc attgcactct tcccaggcgt tttataaaca
      361 aaggaccagg caaacaatga gcatttaaca ccttccgtaa tgaagataat aaaatatcca
      421 cttttgcact cccatgagta agccatactc gggaaagagg cccgatatca ttcgtcattc
      481 cttcaggaat agcatcgcta ataacgagtt gccgcatcag acggtatcga cgacaaacct
      541 tcacaatatg tccggctaat tcaggcaaac catcgtgacg catttttacc catatcacga
      601 tgctactgtc agtcagaaca ttcatatcag tgggtaagga agtgaaaacc tgaagtttca
      661 ctcccccaac acgcttgagc aaactgcaca cgccgcaaat ggcaaaaaga tccaggccaa
      721 tcaccgtgac atttgtccat gtggtatttt ctggcgagag aatattacag ttgatgtttg
      781 tgctttctaa tttcttatac gtcagatcgt taaccattag tttcccccgc gagtaaaacg
      841 ttcgtcattt acaaataccc gagctgcgcg ttttcttatt ttttcagctg tcagataatc
      901 accgcgcctg tgcgcaatga atatcattgc agcgcttacg tctgcatcat tgtggattgc
      961 caaccattta tttccccaca cagtgaaaag agacatgctt tccgtatcgc gaacgaaccc
     1021 cggtttattc gcaataagca gatgctgatc gcgctcaaga cgttctgcct gcgtcaggta
     1081 tttccagcta tgttccggaa ggcgagggat aaatccttcg ttcatatcag cttcagctct
     1141 ggtcagttcc cgctgtgcat caaatcccgc taccaggatg accagcgcgg cggtcataat
     1201 gagcacaatg acaggcagaa tacccaaccg gactgtttta ggtatacgta cctgattcat
     1261 cacgttttcc ggcattccga tgcggaaaag gagcagcaac agcatcaaat ggggaacaga
     1321 cgtatagaga ggatattcgg tcatcatatg aatgactaaa ggcaacccca ttaaggcaat
     1381 aaaccacttt tcacattgat ggtgcgcgtt attgtttgtc attttcaggc ggcatataca
     1441 cggccacagc caaacgcctg atagcagcaa aaaaccaaac aaggcgacga tgccaccttc
     1501 agccgttacg aacagcagtt cgttatgggg atgcgtagcc gtatcactct ctatgtttgt
     1561 cacgcccaca tcctctaagg cttgcgggaa ggtcgcttca aagctgccta acccccaacc
     1621 ggttaagggg cgctgttgaa taagacttat ggcacctttc agaatacgta tacgctggtc
     1681 attcgatgat gaatgcgtaa agtcagccgt tgtctcccct ggcatggcat agtttgagat
     1741 accgacacac cagcacagca aggaggcgac gaagatcaag cgcaacagac cgctaagggg
     1801 acgtccgcgc cagcggggtc tgtccgcgag agcggccagc acgatgacgc aaaataaacc
     1861 aagaaggccg acacggcttt gtgtaaggac taaagctgta gacagagggt ataacgacaa
     1921 caaacatatc aacgtcagag aacgagtacg cgccgttaaa gccagataca acgctacgcc
     1981 gcaacctgtt gccagccagc tcgcaagaac atttcgctgc tggaaaatgc catacggaaa
//
LOCUS       DAZSIO010000333         2034 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZSIO010000333 DAZSIO010000000
VERSION     DAZSIO010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.340"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..2034
                     /gene="sat"
                     /locus_tag="ACHX5T_004236"
                     /pseudo
     CDS             1..2034
                     /gene="sat"
                     /locus_tag="ACHX5T_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          746 a          356 c          401 g          531 t
ORIGIN      
        1 tacggcatga tgatttataa aaatgataaa acatttcgta atcttgaaat attcggagac
       61 agcggctctg gagcatactt atatgataac aaactagaaa aatgggtatt agtcggaaca
      121 acccatggta ttgccagcgt taatggtgac caactgacat ggataacaaa atacaatgat
      181 aaactggtta gtgagttaaa agatacctat agtcataaaa taaatctgaa tggcaataat
      241 gtaaccatta aaaacacaga tataacatta caccaaaaca atgcagatac cactggtact
      301 caagaaaaaa taactaaaga caaagatatt gtgttcacaa atgggggaag tgttctgttt
      361 aaggataatt tggattttgg ttgcggtggt attatctttg acgaaggcca tgaatataac
      421 ataaacggtc agagatttac atttaaagga gcaggaattg atatcggaaa agaaagcatt
      481 gtaaactgga atgcattgta ttccagtgat gatgttttac acaaaatagg ccccggtact
      541 ctgaatgttc aaaaaaaaca gggggcaaat ataaagatag gtgaaggaaa tgttattctt
      601 aatgaagaag gaacatttaa caatatatac cttgcaagcg gaaatggtaa ggtaatacta
      661 aataaagata attcccttgg caatgatcaa tatgcgggga tattttttac taaacgtggt
      721 ggcacgctag atttaaatgg acacaatcag acttttacta gaattgccgc cactgacgat
      781 ggaacaacaa taactaactc agatacaaag aaagaagccg ttctggcaat caataacgaa
      841 gactcctaca tatatcatgg gaacataaat ggcaatataa aactaacgca caatattaat
      901 tctcaggata agaaaactaa tgcaaaatta attctggatg gtagtgtcaa cacaaaaaat
      961 gatgttgaag tcagtaatgc cagtcttacc atgtaaggcc atgcaacaga gcatgcaata
     1021 ttcagaagca cagcgagtca ttgctccctg gtatttcttt gtggaacgga ctgggtcacc
     1081 gttttgaaag aaacagagag ttcatataat aaaaagttca attctgatta caaaagtaat
     1141 aatcagcaga cctcatttga tcagcctgac tggaaaaccg gggtgtttaa atttgataca
     1201 ttacacctga acaatgctga cttttcaata tcacgcaatg ccaatgttga aggaaatata
     1261 tcagcaaata aatcagctat cacaatcggc gataaaaatg cttacattga taatcttgca
     1321 gggaaaaata ttactaataa tggttttgac ttcaaacaaa ctatcagtac taatctatcc
     1381 ataggagaaa ctaaatttac aggtggcatc actgcacata acagccaaat agccataggt
     1441 gatcaagctg tagttacact taatggtgca acctttctga ataatactcc tataagtata
     1501 gataaaggag caaaagttat agcacaaaat tccatgttca caacaaaagg tattgatatc
     1561 tccggtgaac tgactatgat gggaatccct gaacagaata gtaaaactgt aacgccgggt
     1621 ctccactacg ctgctgatgg attcaggctg agtggtggaa atgcaaattt cattgccaga
     1681 aatatggcat ctgtcaccgg aaatatttat gctgatgatg cagcaaccat tactctggga
     1741 cagcctgaaa ctgaaacacc gactatatcg tctgcttatc aggcatgggc agagactctt
     1801 ttgtatggct ttgataccgc ctatcgaggc gcaataacag cccccaaagc tacagttagc
     1861 atgaataatg cgatctggca tctaaatagc cagtcatcaa ttaatcgtct agaaacaaaa
     1921 gacagtatgg tgcgttttac tggtgacaat gggaagttta caacccttac agtggacaac
     1981 cttactatag atgacagtgc atttgtgctg cgtgcaaatc tggcccaagc agat
//
LOCUS       DAZSIO010000334         2030 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZSIO010000334 DAZSIO010000000
VERSION     DAZSIO010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.341"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..889)
                     /locus_tag="ACHX5T_004237"
     CDS             complement(<1..889)
                     /locus_tag="ACHX5T_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFR8469165.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSS"
     gene            complement(1321..>2030)
                     /locus_tag="ACHX5T_004238"
     CDS             complement(1321..>2030)
                     /locus_tag="ACHX5T_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HFR8469166.1"
                     /translation="KPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIRQYPA
                     YYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDKFHIS
                     IAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSDQECS
                     QYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDRDGSE
                     RQEQMLREEPFYRLMIE"
BASE COUNT          627 a          395 c          348 g          660 t
ORIGIN      
        1 cagagcttct cgtggccaga aggttcacca gtttagattg agaaatttca ccatttttgt
       61 atttttccat cagtataaat aatacttctg tcagattctt tgttatcgca tgatatataa
      121 ctctggaatt gatcaattca tctggtaaaa gtttcactgt gtttgttaga ggggaattat
      181 gcctatcttc aaatattacc tgcccgggtt gctccagacc ataaattttc caataaaaat
      241 catcaaaaaa atccttagcg gataattgac tcagttcgaa atggctatca caagtaaata
      301 cagtacgttt atgagtaaca gttcgattag gatcgaaaaa ctgaacaacc catttttcac
      361 tttcttgtac gcgctttatt ttcaacccta acgccatttg gtggtttaat gtgcatgctg
      421 ataatgttct gacaaagtct ccattttttt tcatgttata aaaaaaatta gtaataactg
      481 ttccccactt tttattttcc acaaaataaa tataagctgg tgattttgaa aaatcttccg
      541 ttttttccaa acatacattt tttgcaatag catcaggggt gctaagaagc tgatagttta
      601 ccttcccatt tgtttccata aaattcattt cccaatagga agctaggtga cggcaaacta
      661 tttgtcttcc atatatatct tttgccactc catttatatt tacctccaca tttctctttt
      721 ctgaaaaata aggtgctgtt ttattcttaa actgaattag ttcactggag tttgagaccc
      781 tcataataga acttgttatt gttctattat ctctgttaac agacatatct gttgataacg
      841 aattttttgt tgaaaatata ctagatgaaa atgttttatt taacggcata gaagacaacc
      901 atagagatgt tataatacta ttagattgca gttactaata gtaaaagcct ccttacaagg
      961 aggtagctta ttcagaatgc tcctataaga tagcgagctc gtcaacgata cagatgatct
     1021 gattatattg tgcaaaagga gaacaggaaa ataggcactg gaaaagatga aggcctgatg
     1081 ggagcattaa ctaatgcaaa atccgacagg ttaatatccc gaaaagcaat caaacgactc
     1141 aaacaggaga tgcacgctga aatggtggac aactgttcgg atttcagatg agtagctcag
     1201 cagtttatga gccgaatgct ctgtcacggg caggaatcag gctgcatacg cagtgccaaa
     1261 tctaatcgta gttattctaa ccagttttca agctacaagg tggtgtagct ggcatcttct
     1321 ctactctatc atcaaacgat aaaatggttc ctctcgtaac atttgctctt gcctttcact
     1381 tccatctcga tcgcttcgta gttcattacg atagctgaca tatttccagt cttcaggtct
     1441 aacatctgac gccggatact ccccgggagc gattttgctt cttaatagat tagactcgag
     1501 acacattata aattgtcgta ttttatgaag taataaagca ctatattgcg agcattcctg
     1561 atctgatttt acatatagcg taaactgagc tcctatcccc acacgagatt gctgagaaac
     1621 tcgattcata tcagttatct tccatttatc tataggactg tcctctgaaa ataataaacc
     1681 agacaaaatt tggaatgcca gaggaacttg ctccctagct atactaatat gaaacttgtc
     1741 cccaacaaaa tcccccttac tctgaggatt ttcacgacat gcatgaataa acacatctcc
     1801 actatggttc acaatctgga atccttggta catgacagaa aaagattgac atatatcaga
     1861 gtaagttggc acgttcgatg ctactttgaa atagtacgct gggtattgcc gtatttggtt
     1921 gtacaatata ttgaagttat ttttcaatct ttcattcgca gaaagcatct gcggtatttc
     1981 agacctcaca acattcaaag aatctaaatt tagttttaga cagggctttt
//
LOCUS       DAZSIO010000335         2029 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZSIO010000335 DAZSIO010000000
VERSION     DAZSIO010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.342"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..1824)
                     /locus_tag="ACHX5T_004239"
     CDS             complement(<1..1824)
                     /locus_tag="ACHX5T_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFR8469167.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
     gene            complement(1932..>2029)
                     /locus_tag="ACHX5T_004240"
     CDS             complement(1932..>2029)
                     /locus_tag="ACHX5T_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8469168.1"
                     /translation="WNDEKPETIQPQSRPSSAITVSKTGYVFFDR"
BASE COUNT          485 a          579 c          430 g          535 t
ORIGIN      
        1 gcgcagttct tcacttgctg aggaaaaaac aagcgttcca tccttctcga catacccatc
       61 cgtttcaagc agaccacaca gcatgcctat acgcgttgat ttatttgcgg aaaaaaacac
      121 acgagggatg aatttgtttt ttgctgtgca ccccattagt ccataacctc gcagggtctc
      181 catgagtgga tttacctgac cacgcgccgt tgatatcagc cagtccttac catctccaga
      241 gaagttgtaa tcaggcagtt cagccttcat acgctcgatc atgtatggct cgacgtttga
      301 aaacttcacg ctcccattgc taagactacc atccccgaga agggagccga taacccatgc
      361 taatggctct gagtggtcgc caaagtcacc agttatttca ggaatcctaa cgccattttt
      421 gtgcctcttc gtttcactca accgcttcaa cccgaaggta tctatcacgc ggcgtttctc
      481 gcctttcgtg aatccacgac tagtgacctc ccataggtga tcgcctgcgc aatcaacata
      541 acgaccatct tcaaatgtga ctcggtaaac atctctaaca ccctgcggga atatcccagt
      601 gacagttgac ggattgccgt ctactgacgc cacctgatcg ccaattttta cattcccgtg
      661 agtagtccat gtcccatcag ctaaaagaat aggctcattc agtgggcatg cctttccgtg
      721 accgtgcaca ccattgacca gcgtcaactc tgcctcacgg aactggaatt tatctgccag
      781 agattcccac ggtggattaa acagatactg ctgcttgccg tagaaagcgt tgatagtgtc
      841 ctggtaaaac tctcgcgcac tgtagagttc ttcaggatcg aagtaggatg ccgtgccgat
      901 gtactgccag atttcatcct cggtaacacc gttcatcagg cattcgttga tgtctttgta
      961 cggcagagta acaagacggc aacgatgttc accgagtcgg cttacgattt cccttgcggc
     1021 ttcacgacca acatcatcaa cgtccatcga gatgaatatt tcctcaaacc tgtcgaggtt
     1081 gtgatactca aactcaatcc actgttgctt agcgcctttc ccgccaccaa acggcacgga
     1141 taacgccgag atgccgtatt gcgcatagct catacaatca atttcgcctt cgcaaagcac
     1201 aaccaccctc acgccagcgt ccagagcctg ccatccgaac aggcaaggtt cgcaatcacc
     1261 ttctgccata atgactttct tcccgtccgg gcgctcagtg ctgattcgct tgacctgcaa
     1321 caactcacca tcgcgtttgt acggaagcac cagagcatcc agttctcgtt ctccattcca
     1381 caccttgccg ctgacaacct cgtagcgctt tacgacttct ggcgatatgc cacgcgattg
     1441 caggtactca agatgggatt ctgttctggt aacgtagcgg gcgattttct tacgatcagg
     1501 tctggagaat ttcttctcac gtctggcatc gaaatggtga tcgtcatcct tgataccgag
     1561 aaatgctttc gcttcctgca tagcctgatg caggttaatt ccacgacatg ccatccacaa
     1621 atcaagcatg tcaccgccgt ctccctcagc gaaatcagcc cattttttct tgccgataag
     1681 gttgacctta aggctgtttc ccttgtcacc gttgacgtta ccggcaaccc actcatgccc
     1741 ctctatcttg ccgtttggca acaggtgcgg agccaccctg tcaacctgcg cccaaagcag
     1801 gtcgctaagt tcacttggcc tcatgattcc ctcagattga gatttttaaa ccagaaatcg
     1861 acaaacgaaa tacttaacca gccgtggtta taaccagcga ccagtagcga tttgattttt
     1921 gatttcatgg ttcacctgtc gaaaaacaca tagccagttt tcgatacggt gattgcggat
     1981 gatggtctgg attgtggttg aatggtttct ggcttctcgt cgttccagc
//
LOCUS       DAZSIO010000336         2028 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZSIO010000336 DAZSIO010000000
VERSION     DAZSIO010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.343"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            31..1554
                     /locus_tag="ACHX5T_004241"
     CDS             31..1554
                     /locus_tag="ACHX5T_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR8469169.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            1686..1905
                     /gene="ymgF"
                     /locus_tag="ACHX5T_004242"
                     /pseudo
     CDS             1686..1905
                     /gene="ymgF"
                     /locus_tag="ACHX5T_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
BASE COUNT          536 a          434 c          453 g          605 t
ORIGIN      
        1 tgtattaaac cctttggccg ggacaagcaa atgcgcaata cactcatacc catcctcgtt
       61 gcgatatgct tgtttattac cggtgtcgct attttgaaca tccagctctg gtattccgca
      121 aaagcagagt acctggcggg agcaagatat gccgccaaca atatcaatca tatacttgaa
      181 gaagcgtcac aggcgactca aacagcggtt aacattgccg ggaaggaatg cgacctcgag
      241 gagcaatatc agcttggcac tgaagcagct ctgaaacctc acctgcgcac aatcatcatt
      301 ctcaaacagg gaatagtctg gtgtacatcc ctgcctggga atcgggtcct gctgtctcgt
      361 attcctgttg tcccggacag taatttactg ttggctccgg caatcgacac cgttaataga
      421 ttacctatcc tgctctatca gagccaattt gcagatacgc gcattttggt tacgataagt
      481 gatcaacata ttcgcggggc gcttaatgta cccttgaaag gggtaaggta tgtattacgc
      541 gtggcggatg acattattgg accaacggct gatgtgatga cgcttaatgg acattatccc
      601 tataccgaga aggttcactc cacaaaatat catttcacta ttatctttaa cccgccgcca
      661 ctctttagct tctacagact tatcgataaa ggctttggga ttttaatatt tattctgtta
      721 atcgccagcg ccgctgcctt cctgcttgat agatatttca ataaaagcgc aacgcctgaa
      781 gagatcctgc gacgggcaat aaataatggg gagatcgtcc ctttttacca acctgtggta
      841 aatggtcggg aagggacatt gcggggagtt gaggtgttag cccgctggaa acaacctcac
      901 ggtggatata tttcacccgc ggcatttatt ccacttgctg aaaaatccgg attaatcgtt
      961 ccgcttacac aaagcctgat gaatcaggtt gccagacaga tgaacgctat cgcgagtaaa
     1021 ctgccggaag gttttcatat tggaattaat tttagcgctt cgcatattat ttcgccgacg
     1081 tttgtcgacg agtgtttaaa tttccgtgac agttttaccc gccgcgattt aaaccttgtt
     1141 ctggaagtca ccgagcgtga gccattgaat gttgatgaaa gtctggttca gcggttgaac
     1201 attctgcatg aaaatggttt tgtcatcgcg ctggatgatt tcggtactgg ctactcaggg
     1261 ctttcttatc ttcatgacct gcatattgat tatatcaaaa ttgatcacag tttcgttggc
     1321 cgcgtaaacg cagacccaga atcaacccga attctggatt gtgtattgga tctggcgcgt
     1381 aaactttcga tcagtatcgt cgctgaaggt gtcgaaacga aagaacaact tggctatctg
     1441 aaccaaaata atatcacatt tcagcagggt tattatttct ataaacctgt tacatacatc
     1501 gacctggtca aaattatcct ttctaaaccg aaggtgaagg ttgtggttga gtgaaaattg
     1561 atcagtaagg ccatagtgcg gtgtaattat agacagctaa ttagctcgtt gcctcttgtt
     1621 actattgttc attattttgt ttgctataat tgtttgaaag ttttgacagg attgccatta
     1681 gtagcatgaa taatagtaat aatctggatt atttcactct ctatatcatc ttttccattg
     1741 catttatgct gatcaccctc ctggtcatcc ttattgcaaa acccagtacc gggctgggag
     1801 aagtgcttgt gacgataaat ttgcttaatg cccttgtttg gctggcgatc aatctggtta
     1861 atcgatttaa gagaaagact cgttagccac agggatcagc aataatcttt cagtttctca
     1921 ctgtcagtat gcggctgaat gggttgctgg cagtgaacgc ctggatcatt gaaggaaagg
     1981 aattattgcg caaatagttg tcaaccttgg tgttatcaca gttgtttt
//
LOCUS       DAZSIO010000337         2014 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZSIO010000337 DAZSIO010000000
VERSION     DAZSIO010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.344"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..892
                     /gene="virK"
                     /locus_tag="ACHX5T_004243"
     CDS             <1..892
                     /gene="virK"
                     /locus_tag="ACHX5T_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR8469170.1"
                     /translation="DSLPGKWEHRKFRFMYILRCSINPVVSIRYYYELRSLPCIEDIL
                     AIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNLPEKYSKFLFPQKSVSLVQFI
                     GKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTFSVILTQNGHIAFIGGLQGAP
                     KNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNISECLAVSEHSHVFRQLRYWYQ
                     KRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSDIASKKRSGYRKRYALLDYIH
                     ETTIRSLDAYPVHSEHQNLN"
     gene            957..1901
                     /gene="lpxM"
                     /locus_tag="ACHX5T_004244"
                     /gene_synonym="msbB"
     CDS             957..1901
                     /gene="lpxM"
                     /locus_tag="ACHX5T_004244"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR8469171.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          594 a          388 c          456 g          576 t
ORIGIN      
        1 agattcacta ccggggaagt gggaacacag aaaatttcgg ttcatgtaca ttttgcgatg
       61 ttctataaat ccggttgtca gtattcgata ttattatgaa ctgcgttcct tgccgtgcat
      121 tgaggatatt ctggcaatac accccacgtt gccagcaaga attcatcgcc cttatttgca
      181 taaagggggg cgcgcctgga cccgtggaca atatattctt gagcattatc gctttgtgca
      241 aaatttgcca gaaaaatatt ctaaattttt gtttccacaa aaatcggtat cattagtcca
      301 attcatcggg aaagatggag agaattttga tatccagtgt tctcccagtg gttttgaccg
      361 ggaaggagag ttgatgttgt cattgtttta caataaaaca gttattgccc ggttaacatt
      421 ttcagtcatt ctgactcaaa atggacacat tgcttttatt ggcggattac agggagcccc
      481 taaaaatacc ggacctgatg ttattcggtg cgcaacccgg gcctgttatg ggattttccc
      541 aaaacggatt atttttgaag cattttgtgc gttgatgaaa gcttgcaata tctctgagtg
      601 tctggcagtc agtgagcaca gtcatgtttt ccggcaattg agatactggt atcagaaacg
      661 caaaaccttt gttgcggtct atagtgattt ctgggagtct gtagcaggaa aaacctgtgg
      721 tgactggtac agattaccaa cccaggtgat tcgaaagccg ctaagcgata tagccagtaa
      781 aaaacgctct gggtaccgaa aacgatacgc tttgctggat tatatccatg aaactactat
      841 ccgctctttg gatgcatatc ctgtacactc agaacatcag aatttaaatt aaggtttgat
      901 gtattctctg aataaaacag taatgataat tttggtagag gcattcgcac taaataatga
      961 aaaaatataa atctgagttt attcctgaat ttaagaaaaa ttacctttcc cctgtttact
     1021 ggtctacatg gttccttttg ggaatgattg caggtatttc aatgtttccc ccttcattca
     1081 gagatcctgt cttggcaaaa atagggcgtt gggcgggcag attgagcaaa aaagctcgtc
     1141 gcagggcgac gattaattta tcgctttgtt tcccagaaaa gagtgataca gaacgggaaa
     1201 taattgtcga caaaatgttt gtcacagcat tgcaatccat agtgatgatg gcagaactgg
     1261 caattcgtgg tccggaaaag ttccagaaac gagtgttctg gaaaggcctc gaaattcttg
     1321 aggaaattcg acacaataat agaaatgtga tttttctggt tccccatggc tggagcgtgg
     1381 atattcctgc aatgttgctg gcagctcagg gaaaaaaaat ggccgccatg tttcatcagc
     1441 aacgaaatcc agtgattgat tatgtctgga attcagtacg gcgtaaattc ggggggcgct
     1501 tacatgcccg ggaggatggg ataaaaccat ttattcagtc agtacgccag ggatactggg
     1561 gatattacct cccagatcag gatcatggtc ctgaatacag tgaatttgct gatttttttg
     1621 cgacctataa agcgacatta ccaatcattg gacgtctgat gaacatcagt caggctatga
     1681 ttataccact tttcccggtt tatgatgaaa aaaaacatct actgactatt gaaattcggc
     1741 caccaatgga tgcttgcatt gccagcgcgg acaataaaac gattgcccga caaatgaaca
     1801 aaacagtgga aattttggtg gggccacatc cggaacagta tgtctgggtt ttaaaattgt
     1861 taaaaacacg caaatcaaac gaagcggacc cgtacccttg aaattataaa gtacaagtat
     1921 ttccactagt tgtttgtaag cgtacagcct gaaccgtctg gtcagaatct gacgaattag
     1981 acaaagtggt gtccaccaaa taagtagtgg gaac
//
LOCUS       DAZSIO010000338         1976 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZSIO010000338 DAZSIO010000000
VERSION     DAZSIO010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.345"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(1..837)
                     /locus_tag="ACHX5T_004245"
                     /pseudo
     CDS             complement(1..837)
                     /locus_tag="ACHX5T_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          533 a          465 c          457 g          521 t
ORIGIN      
        1 agctgctcta cggtcatatt tttaattgcg ctccggttta caagagccca cccctgtttt
       61 tccagataaa actggaaagt atccagggta cagaccagtg caccgtcagg aacagtttcg
      121 gtgaatctga tattgccgtg ttcgtcgaag tgcacaacca gagtgcgacc gttacccgga
      181 atcatcttgt cagcggacgg ggtattattc tggtgcagtt ctgcctccat gcggtcgaac
      241 atcagcgatg taggcctctt tgaatgcagc ggcttttttc ccagtgaagc ccatcaccag
      301 gaaaacgaag ccgtttttgg tgatttggta cattgggcgt ttttcgcctt tggtgtcggt
      361 gtaggtgacg tcctcaaaat tgagggcgtt aaattcaggt gagcattcag tatttgcgat
      421 ggcacgtaac acattgtcgt gtcgtttgtg gaagaactct gcaaccgcaa cagacgtagt
      481 gacagcgcga ccgttttcga tggttacgtc agggtgagaa agggcaggga tagtagccat
      541 gatggcatcc tcgagtgata agttaattaa ctcaccaccg aggttttcca cgaccataag
      601 ggtggtgaga cgtacagggg tggaaatacc ggtcactcga gaacccggcc agtcttgcga
      661 ctgccctgca catcccacca taatttgaat ggagctgtgc attacgcata aaaaaaccgc
      721 ttcagcgcgg ctatgcgctc gagtaacgtt cgggtttcca cgcccggccc ccgttttatg
      781 aggtgcagat gcactataat tccacccgtt ctggttttca atagctacat tcaacatttt
      841 ctcttacctt tcatcaccga agtgaacttt gttgatgcgg tgcctggtgc ctccaggtga
      901 cgttaaccag ttaacaatta acgccggata cagggaaacc cataaccacc cgatacgtgg
      961 gaactaatcg ctttttaact gttccgcgtg cgctcagccg cattcaccgc atcacaaaat
     1021 tcactttaaa aagggcggac atcagccagc aatgaaactg atgccgccaa aggtaccaat
     1081 caacatggag tgttgtggcg tggttgtcac ttaagcgtat ggtcaacctg acaacccggt
     1141 gtcctcaacg gggaaggaat aactccgcca tacttaccgc cgcgtcattt cgcgttgtgt
     1201 gcctgctttt aactacgtca ggcgaggtgg ttcctgttat tccccaacaa caagaaattt
     1261 gtataatctg aataccccaa caataagaag agtgagcaag tgatgtatta gcgatgtcag
     1321 ctctcaaaga gactgcatca atgttaaaat tattcaatga agcaaaaact caggctgagg
     1381 ttgatcgcgc tgtgtctgaa ataaactcta agttgtcaag cattcagctt gaatatttgt
     1441 catctcattg atgagctgac aatctctaaa agcaatgagg ctgcgctcaa agcaaaactc
     1501 acggagatag aaaagtttaa gttccaaagt gaagactgcg aacctcgcta attgattact
     1561 ggaacgattg tgtatgccag acagtgcaat actagctctg gcaagattac catgtatttt
     1621 tgtccagaat gttttgcgaa gagagcaata tctatattgc aacctttccc tatcgaggaa
     1681 ggcgataaat atacgaaaac tcaatgcccc tcctgtaaaa atgagtatct catggactac
     1741 aatttcaata atgatttttg agtcacggta ttcagatttt taaagaacat gccggatact
     1801 cacccgtgtc cggcgcacgc actccacctc acccgtggag aactccttaa ttaccaacct
     1861 tggcttcgtt ggttagccat taacgctggt atgtaatcat tctggcaatg cttaatttga
     1921 agtggcacac tgaatttggc cacctgaaca gaggtgatat gctcacctca gaacaa
//
LOCUS       DAZSIO010000339         1969 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZSIO010000339 DAZSIO010000000
VERSION     DAZSIO010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.346"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            240..509
                     /gene="yacA"
                     /locus_tag="ACHX5T_004246"
                     /pseudo
     CDS             240..509
                     /gene="yacA"
                     /locus_tag="ACHX5T_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
     gene            509..678
                     /gene="yacB"
                     /locus_tag="ACHX5T_004247"
                     /pseudo
     CDS             509..678
                     /gene="yacB"
                     /locus_tag="ACHX5T_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="ACHX5T_004248"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="ACHX5T_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HFR8469172.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1708..>1969
                     /locus_tag="ACHX5T_004249"
     CDS             1708..>1969
                     /locus_tag="ACHX5T_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR8469173.1"
                     /translation="MTEALRLHFEEHLPRVVAGCRLGVPKSTACGMFVRFRNAGLSWP
                     LPAGMSEQELDALLYGSASTVPVVLTESTVMPKLPVVKKRPRR"
BASE COUNT          560 a          433 c          462 g          514 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
     1561 acaaggcgaa tcacgcacgg atttccagcc agagacacct gaaacgtaac cggctcattt
     1621 aaaccgtctg gtctgttacc tccggttcca caaaaataat gtccatcatt ttgaagtgga
     1681 cactatcgta tggaataccg gacctgaatc actgaagctt tacgccttca tttcgaagaa
     1741 catttaccac gggttgtggc cggatgtcgc ctgggcgtac caaaatcaac tgcctgtggt
     1801 atgttcgtgc gcttccgcaa cgctggcctt tcatggcctc tgcccgcagg tatgtcggag
     1861 caggaacttg atgcccttct ttacggaagt gcctccacag tacctgtcgt acttactgaa
     1921 agcaccgtta tgcccaaact cccggtggta aaaaaacgtc cccggcggc
//
LOCUS       DAZSIO010000340         1966 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZSIO010000340 DAZSIO010000000
VERSION     DAZSIO010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.347"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..414
                     /gene="torD"
                     /locus_tag="ACHX5T_004250"
     CDS             <1..414
                     /gene="torD"
                     /locus_tag="ACHX5T_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR8469174.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(507..812)
                     /gene="cbpM"
                     /locus_tag="ACHX5T_004251"
     CDS             complement(507..812)
                     /gene="cbpM"
                     /locus_tag="ACHX5T_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR8469175.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="ACHX5T_004252"
     CDS             complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="ACHX5T_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR8469176.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT          466 a          507 c          502 g          491 t
ORIGIN      
        1 acgctgacag tacgtgacga tgcccgtctg gaactggccg ctgacttttg cggcctgttt
       61 ctgatgaccg acaaacaagc ggcgctgccg tatgcatcgg cctacaaaca ggacgagcaa
      121 gagattaaac gcttgttagt tgaggcaggg atggaaacca gtggcaattt caacgaaccg
      181 gcagatcatc tggcgatcta tctcgagttg ttgagttatc tgcatttttc gctgggagag
      241 gggaccgttc ctgcgcgaag aatcgacagt ttgcggcaaa aaacactgac ggcgctgcgg
      301 caatggttac cagagtttgc tgcgcgttgt cgacagtatg acagctttgg tttttacgcg
      361 gcactaagcc agttattgct ggtgttagtg gagtgcgacc accaaaacag ataacgtcgt
      421 ttatgcgcct gaaaagacgc gtttagcgtc gcatcaggca ttatggcgca gttgccggat
      481 gcgtcatcac atccggcaaa cacacatcac ggatgagcta caaaccggga aagccgctgg
      541 cgtagcaggc ggttttcctg cttcaggtgc gcaatatcat ccattaacgt cagcgccacc
      601 gcgatccccg gccagtccag agccagttca tgacgcaggc gtaccgcgcg ttgcaccaca
      661 atggcggcat ggtcgtcaaa tacccaggtt gtttcctgga tctcacgcgg ttcaaccacc
      721 cccaaaccga caatttcatt caactcctct tcagagatgc cggtatgcag gcaaaattcg
      781 gtaatagtaa aagtcaccgt aacattagcc attatgcttt cccccaatct ttacgtggat
      841 caaaagacga ctgggcgtct gccagttgct gccacagcgc ggcagtgttt tcatccggtt
      901 tcggcggcat cacgattttc agtaccgcat acagatcgcc ggtctgtttt ttgctcacca
      961 gacctttgcc tttaacgcgc aatcgttgcc cggcctggct gcctggcggg atagtcagca
     1021 aaatgctttc tttcagtgtt ggaacggtga ctttagtacc cagcgccgct tcccacgggc
     1081 taaccggcac cacaatttcc agatcctggc cgacaatatc gaacagcgga tgtggcgcaa
     1141 tatgaatcac cagccacaaa tcgccatttg gaccgccgtt ttcgcccggc gtcccctggc
     1201 ctttcaggcg gatgcgttga ccattgccga cgcctgccgg gatcttcaca ttcagcgttt
     1261 tcgggatttc ctgttcgatc atgccaaagg cgttataaac cggcaggtta tagctgatgg
     1321 tacgcttatg ctcagtaagc gtttcttcga ggaataccgc cacttcgatt tcaatatcgt
     1381 ggccacgtgc ggcggggcgt tgacggctct ggcgggcatg ctgaccgaaa attgacgaga
     1441 agatatcgtc aaaatcttcg gcattaaaac tctgaccgtc gccatgatgg aactgacggt
     1501 taaattgcgg atcgttgcga tgttgccaca tctgatcata ctcagcgcga cgttgttcat
     1561 cacttaacac ttcccaggct tcagcgacct ctttgaagcg ggcttcggca tccggttctt
     1621 tgctgacatc aggatggtat ttacgggcca gtcgacgata ggcggtcttg attgtcttga
     1681 gatcgtccgt cggtttcacg cccatgatgg cgtaataatc ctttaattcc atagcgttat
     1741 ctcgcgtaaa tcaacacaaa ttgaaggaac ccctgtaagg taactcctat aagtgtaggg
     1801 taatcctcaa aatttcatat gccaacacag aatatgttat tgaaatcatc gcggagagga
     1861 ggtcgccatc aagatgggtt gctgaacata ttttaaacag gtgaaaaagg gtgagcgatt
     1921 tttgatagtt gaaccaggca ctttaagttt aactagggcg tcatta
//
LOCUS       DAZSIO010000341         1960 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZSIO010000341 DAZSIO010000000
VERSION     DAZSIO010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.348"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..494)
                     /locus_tag="ACHX5T_004253"
     CDS             complement(<1..494)
                     /locus_tag="ACHX5T_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR8469177.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(525..1217)
                     /gene="fimC"
                     /locus_tag="ACHX5T_004254"
     CDS             complement(525..1217)
                     /gene="fimC"
                     /locus_tag="ACHX5T_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFR8469178.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1437..>1960)
                     /gene="fimA"
                     /locus_tag="ACHX5T_004255"
     CDS             complement(1437..>1960)
                     /gene="fimA"
                     /locus_tag="ACHX5T_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFR8469179.1"
                     /translation="SALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVNTDSADQV
                     VTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAINDNLLAIVS
                     STNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGTGTSASAG
                     QANADATFIMRYE"
BASE COUNT          568 a          414 c          426 g          552 t
ORIGIN      
        1 tgagcgctac tcaacaacgc tatctgagga atagtgatgt tcaggcgcat cgctgcaaaa
       61 tcaaagttaa ttgtggcatc aggtacagct ttcagtaaat tgatgcattt attgttttgc
      121 tgctgggcta actcgggaaa tgcactgcta ttaaggccaa ttcgttcaag gagtacctgg
      181 ttaaaacagg gcattaaccc acctgattta tcacctgtat tttctgttgt cgattcaaat
      241 acgatatcct gtgaaccaat gaactcatca ttacgccaga gatccacccg atacacgccc
      301 gcaggttgat gattacctct ctcaaagcgc gataaatcag caacggatgt gccattctct
      361 aataaaaatg ccgggttgaa ataactttcc gcatagctta aaaaggaggg taaaatggca
      421 agagatgaat aacaaattcc ggccagacac caggtatacc tctgcggaat atccgtagta
      481 gtgggtattt tcataattag ctctgatatt gtcagatgat tcggttagtt taagttcact
      541 tctctgaccg gggttactga accataatca ttaacggtct gaaatttcag ctttccctgc
      601 actcctgagg gtaagggaat ttgtgaacta actctgggtg ccgccatagc attccccaac
      661 ttttggctgc ccacttgcag gttaacgagg gtgacataaa aaggtgatgg attatgaaca
      721 ttgatatagt taccggatcg cgaaaacttg agtgtgtccg gcgcttctgc gggtaattct
      781 tgtaattgaa ttggacggag aaataatttc atgcgcgata aaatcgccag ttgcaaaaca
      841 ttcctgccgt tcaatgcatt tttatctacc gaagggatcg tcttaacatt catccagaac
      901 agagactcac gatctgccgc cagcggtgga ccggtgtaaa taatacgcaa agtattttcg
      961 cttttgggtt cactaacaaa tagtggcggt gtaatgatga atgacttttc ttttacaccg
     1021 ctgctgtttt caatccacga attgaccaga aagagctcat tggtatggct atttttaatc
     1081 cataccgcag tctgtttagc atcagcggga taaataatac gcgtggcacc taatgcgatc
     1141 cctccggcag catgggcgct ggccgaaatg attaaataaa atatcatgag catcaataac
     1201 tttattttag tcatcatagt taatacgcct tgtgtattcc tgacggcaaa aataaggagt
     1261 ataaagaaat gattgctttg gcttcttaca acacatcatt cccttttcat ttaataaagc
     1321 ggcatgatac aaagtgaacg tggtttcgat ttacattgcc accataccgc ttcagacaac
     1381 cggtacatta ctttgttata agacgtgata tataattcaa aacaacgtgg ttttgattat
     1441 tcgtatctca taataaaagt cgcgtcagca tttgcttgcc ctgctgatgc acttgtaccg
     1501 gtgcctttat aacgtgcaga aaaatgaaga acgttggtcc cggggatcaa gttctggttg
     1561 gttgagaagc tattcccatc aggtttgaga attgcggatg tattatcaag tatttcaata
     1621 ccgacacccg ttgctgttgt tgtatttgta ctggatacaa tggccaataa attatcattg
     1681 attgcatcag cctgaccaga aaatgcaacg gcagcattag cggcaacaac aggatcgcag
     1741 tcgttcaact gaatggtgaa tggaattaat gcagaggtat taccaacagc attgaaaata
     1801 tcggtacgat attgaccgag tgtgacaacc tggtctgccg agtcagtatt cactgaacag
     1861 gcagcattca ccagttcgcc ttcaaagtga attgtaccgc catctacaac ggcagcataa
     1921 ctacccgtag cggcgaatag tgcggcagcc agcgcagacg
//
LOCUS       DAZSIO010000342         1946 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZSIO010000342 DAZSIO010000000
VERSION     DAZSIO010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.349"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..203
                     /locus_tag="ACHX5T_004256"
     CDS             <1..203
                     /locus_tag="ACHX5T_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469180.1"
                     /translation="GASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAI
                     YLLAGCLISAMTALLMKDNQRS"
     gene            305..1759
                     /gene="amn"
                     /locus_tag="ACHX5T_004257"
     CDS             305..1759
                     /gene="amn"
                     /locus_tag="ACHX5T_004257"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR8469181.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          486 a          483 c          449 g          528 t
ORIGIN      
        1 ttggtgccag ttatcgctac agtggtgctg gagtcggtta tcaggttgcc agtgtggttg
       61 gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc gggaactggc
      121 atagcatcgc catttatttg ctggctggat gcctgatttc tgcaatgacc gctttattga
      181 tgaaagacaa tcaacgctcg tgatagcctg gcgaagatca tccgatcttc gccttatact
      241 tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac
      301 acacatgaat aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc
      361 gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaaatata ttacaattgg
      421 cgaattacct gatgaaaacg cccgtaaaca aggtcttttt gtctatccat cactgaccgt
      481 aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc gttttactca
      541 cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt atctgaatga
      601 gcaactaacg ttgctttatc aggattatgg tgcgcatatc tctgtgcaac cctcgcaaca
      661 tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc gctcaatgag
      721 cgctgattta actcgctact tcccgacaac agaactggcg caaatcggcg atgaaactgc
      781 agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg cgcgccgcgt
      841 cgatttttcc ctcgcacggt tgcgccatta taccggtacg ccagtagaac attttcaacc
      901 gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag
      961 ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg ggaactggat
     1021 cactgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca
     1081 gatgccagca tggcatttaa ttaccgctga tggtcagggt attactctgg tgaatattgg
     1141 cgtgggaccg tccaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatat
     1201 ctggttgatg attggtcact gtggcggatt acgtgaaagc caggccattg gagattatgt
     1261 ccttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc cgcccgatat
     1321 acctattccg agcattgctg aagtgcaacg tgcgctttat gacgctacca agttagtcag
     1381 cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca ggtactgtgg taaccacaga
     1441 tgacaggaac tgggagttac gttactcagc ttctgcactt cgttttaact taagccgggc
     1501 cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc
     1561 atacgggaca ctactgtgtg tttcagataa accgttacat ggtgagatta agcttcccgg
     1621 ccaggctaac cgtttttatg aaggcgctat ttccgaacac ctgcaaattg gcattcgggc
     1681 gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac gaacctttaa
     1741 tgagccgccg ttccgataat aagttatgaa ccttcttatc agaaatagat gtgaaggatt
     1801 aagtaagaca tctggcaaat agcctgcaat aaacgcgggc tgcttgcttc cttgggcaga
     1861 tgcgagtttt attatcgtct taatgatttc cacatattaa aagcaagtat gctttcaaaa
     1921 cacaattata aaaaatcccg ccaaca
//
LOCUS       DAZSIO010000343         1941 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZSIO010000343 DAZSIO010000000
VERSION     DAZSIO010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.350"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..794
                     /locus_tag="ACHX5T_004258"
     CDS             <1..794
                     /locus_tag="ACHX5T_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shiE"
                     /protein_id="HFR8469182.1"
                     /translation="SETKAAYRCFSNDKVSANKIMTPHKENITERAQQFKRVLVLQDT
                     TELNYSGQKEKQGVGPKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKN
                     TRQEICNDLLHKKHVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFER
                     AAQTPGIKADWLVRMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQ
                     ARTLPDMLSSDAGPLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="ACHX5T_004259"
     CDS             complement(744..1937)
                     /locus_tag="ACHX5T_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8469183.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
BASE COUNT          553 a          496 c          481 g          411 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat a
//
LOCUS       DAZSIO010000344         1925 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZSIO010000344 DAZSIO010000000
VERSION     DAZSIO010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.351"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..164
                     /gene="spa15"
                     /locus_tag="ACHX5T_004260"
                     /gene_synonym="spaK"
     CDS             <1..164
                     /gene="spa15"
                     /locus_tag="ACHX5T_004260"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HFR8469184.1"
                     /translation="FSYSINELVELHRSDEYLQLRVVIKDDYVHDGIVFAEILHEFYQ
                     RMEILNGVL"
     gene            168..1460
                     /gene="spaL"
                     /locus_tag="ACHX5T_004261"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             168..1460
                     /gene="spaL"
                     /locus_tag="ACHX5T_004261"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HFR8469185.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            1541..1891
                     /gene="spa13"
                     /locus_tag="ACHX5T_004262"
                     /gene_synonym="spaM"
     CDS             1541..1891
                     /gene="spa13"
                     /locus_tag="ACHX5T_004262"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HFR8469186.1"
                     /translation="MYRDVEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVK
                     KHIIQYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLI
                     LDELSQEDMKYGIR"
BASE COUNT          621 a          285 c          426 g          593 t
ORIGIN      
        1 actttagtta tagcattaat gagctggtag agcttcatcg ttctgatgag tacttacagt
       61 taagagttgt tattaaagat gactacgttc acgatgggat tgtttttgca gagattctgc
      121 atgagtttta tcagaggatg gaaatcttaa atggggtctt ataatcaatg agctatacaa
      181 aattgctcac tcaattatct tttcctaata gaatctcggg gccaatcttg gaaacaagtc
      241 ttagcgatgt ttcgattggt gagatttgta acattcaggc tggaattgaa agtaatgaaa
      301 ttgttgcaag agctcaggtt gtaggatttc atgatgaaaa aacaatatta agcttgattg
      361 gaaattctcg tggactttca cggcaaacgt tgattaagcc cactgcccag tttcttcata
      421 cgcaagtcgg ccgtggatta ttgggagcag tagtcaatcc tttaggggag gttactgata
      481 agtttgctgt tacagataac agtgaaattc tttatcgacc tgtagataat gctcctccgc
      541 tatatagtga aagggctgca attgagaagc cttttttaac aggtattaag gttattgatt
      601 ctttactcac gtgtggtgaa ggacagcgaa tggggatttt tgcgtcagct ggttgtggca
      661 aaacttttct catgaatatg ctcattgaac atagtggtgc tgatatatat gttattgggt
      721 taattggtga gcgaggtcga gaggttactg aaacggttga ttatttgaaa aactctgaga
      781 aaaaaagcag gtgtgtttta gtatatgcaa cttcggatta ctcttcggtt gatcgttgta
      841 atgctgcata tatagccact gctatagccg aattttttag gactgaagga cataaagtag
      901 cgctttttat tgattcatta acaaggtatg ccagagcatt acgtgatgtg gccttagccg
      961 ctggagaatc acctgccaga agaggctatc cggtttcggt ttttgatagc ttacccagac
     1021 ttcttgaaag gccaggaaag ttaaaggcag gtggctctat tactgcattt tacactgttc
     1081 ttttggagga tgatgatttt gctgatccat tagctgaaga ggtaagatcc attttagatg
     1141 gacatatata tttgagcaga aatctagccc aaaaaggaca atttcctgca attgattcct
     1201 taaaaagtat aagcagggta tttacacagg ttgttgatga aaaacatcgt attatggccg
     1261 ctgcatttcg ggagctactt tctgaaatag aagagctaag gacaattatt gactttggtg
     1321 aatacaaacc gggggagaat gcctctcagg ataaaatcta caacaaaata tctgttgttg
     1381 agagttttct gaagcaagat tatcgactcg gttttactta tgagcagaca atggagctta
     1441 ttggtgaaac aattagataa ggttttaaaa atcaaagata aatatcaacg gtctgttaaa
     1501 ttaattgaag cacacatatt aacactaaaa aaaaaactct ttgtatcgag atgtggaggc
     1561 attagataaa aggattatat acttcctgca actggagaat gacttagaac ctgtaggcgc
     1621 tcagagtgtt agtcagcttt tcaatacaag acgtaagatc gctatagtaa aaaagcatat
     1681 tatccaatat cagtctgaac gaattcttct aaagggaaga attgaagaaa ttcaaaaaga
     1741 tattgatgag gcaaatgctt ctaaaagaaa actgcttcat aaagagagta aaatatgtaa
     1801 acggattggc ttgattaaaa gaaataactt tgccaaacag ttgatacttg atgagttatc
     1861 acaagaggat atgaagtatg gcattagata atataaacct aaattttagt agtgacaaac
     1921 agata
//
LOCUS       DAZSIO010000345         1913 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZSIO010000345 DAZSIO010000000
VERSION     DAZSIO010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.352"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(53..1702)
                     /locus_tag="ACHX5T_004263"
     CDS             complement(53..1702)
                     /locus_tag="ACHX5T_004263"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR8469187.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          452 a          506 c          491 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accatcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DAZSIO010000346         1902 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZSIO010000346 DAZSIO010000000
VERSION     DAZSIO010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.353"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(517..642)
                     /locus_tag="ACHX5T_004264"
     CDS             complement(517..642)
                     /locus_tag="ACHX5T_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFR8469188.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGVTSRMLSVIPLLFFQG"
     gene            1136..1675
                     /locus_tag="ACHX5T_004265"
     CDS             1136..1675
                     /locus_tag="ACHX5T_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HFR8469189.1"
                     /translation="MLEQQFEDFEQQKRRDHQVQDQSKNAKQKRDRLNPPDWSQVTKA
                     GWNMLDAISEKVPRAFRLYSFFAQNIDKSCGAVVCDQKFLADYFGVSLRTIQYWIGDL
                     ESIGAIVKIPVGIYYAYALDPYQVWRGYNNTKKYAAFNAKTLTQKDGVIDRKLRLMLS
                     RREEFESSLKYSNHDSEQG"
     gene            1682..>1902
                     /locus_tag="ACHX5T_004266"
     CDS             1682..>1902
                     /locus_tag="ACHX5T_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469190.1"
                     /translation="MRIKYESRWRLEELKLEICSSIDELINKYGIEYATGINLYMNCE
                     DKEFNLVDVINEDGNKIMFVVSNDEKIQKP"
BASE COUNT          580 a          382 c          456 g          484 t
ORIGIN      
        1 aaaaaaaata gcaaagaaaa aaactccagc acctgtaatt gagctagata aatatcgaaa
       61 aaaatgacca ttgagccagc ggcaggcgga caatcagggg ctacgtgtta acgttctgac
      121 cttgattgtc tatcctgcat tgctcttttg ccgcctcgaa tcccttgcgt gtttttgttc
      181 cacgctctcc agaacgaccg agccgccacg gttccgatag cgccttgagc gcaaccggaa
      241 accgtctggg gagaacccca gacccccatc gctgtaatac cgacggtcta cgaccaccta
      301 ccagccgaat agctgcctat gccgtgcaaa taggctttgc agggggctga cgctgttcct
      361 ggcgacctga aagcccctgg ttaggggtat cggcgcgcct gacctcctga gagcctctgt
      421 aagcgttttt cgcgccttcc atgccctgac gggcatttaa aacccgtttc gtgcgtctga
      481 gagcttctga gcgcgtttga agggctatct gacgactcat ccctgaaaga acaacaacgg
      541 gatcaccgac aacatgcgag aggtgacacc gctcgccgca agtcgatgac cgagcgtagc
      601 gagcgaatcg acgaggaagc ggaagagaac cggaagccac attgagcact tacgcactga
      661 tgcggggtgt cggggtgaag ccctgaccag gtggtgaatg tctgagtgcg cgtgcgcggt
      721 ccgacattcc cacatcctgt cccgatttct gaggcttttt agcggtgaac ggacggcaaa
      781 accatgatgg agttgttgaa cgttgagagc atgaaatctg gcaaaaatca cggctggtaa
      841 ggcttgcaga ggagtaagcc agtatacact ccgctatcgc tacgtgactg ggtcagggct
      901 gcgccccgac accccaaaag cagttaggta gtgttaaatc atgagcagta aaaaaacgct
      961 ttgatagatc tttagagcaa aaattgacat ttctttaaaa atcattgact taagacacct
     1021 tcatacgaaa cgttatttgc atgtgtatac gcaaacatgt ttgcatgtgt tgtttcgtac
     1081 tgtacgatat aaatttcgta aggtgcgaaa tatcattgca ggtaaggatg caagtatgtt
     1141 ggaacagcaa tttgaagact ttgaacagca aaaacggcga gatcatcagg tacaagatca
     1201 atccaagaac gctaagcaaa aacgagatcg gttgaatcct ccagactggt cgcaggttac
     1261 taaggctggt tggaatatgt tggacgctat atcagaaaaa gttcctagag catttcggtt
     1321 atattctttt tttgcccaga atatagataa atcatgtggg gctgttgtat gtgatcaaaa
     1381 attcttagct gattatttcg gtgttagttt aagaacgatc caatattgga ttggagattt
     1441 agaaagtatt ggcgcaatag ttaaaatacc agtaggcata tattatgcct atgcattaga
     1501 tccttatcag gtatggcgtg gatataacaa tactaaaaaa tatgcagcat ttaacgcaaa
     1561 aacattaact caaaaagatg gggttataga tcgtaaattg cggcttatgc tttctaggcg
     1621 tgaagagttt gaaagttcct taaagtactc taatcatgac tcagagcagg ggtgatgcac
     1681 tatgagaatt aaatatgaat cacgttggag attagaagaa ctaaaattag aaatatgcag
     1741 ttcaatagat gagttaatta ataaatatgg tattgaatat gcaacaggaa taaatcttta
     1801 tatgaattgt gaagataaag aatttaatct tgttgatgtt atcaatgaag atggaaataa
     1861 aataatgttt gtcgttagca acgatgaaaa aatacaaaag cc
//
LOCUS       DAZSIO010000347         1883 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZSIO010000347 DAZSIO010000000
VERSION     DAZSIO010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.354"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..612)
                     /gene="spa29"
                     /locus_tag="ACHX5T_004267"
                     /gene_synonym="spaR"
     CDS             complement(<1..612)
                     /gene="spa29"
                     /locus_tag="ACHX5T_004267"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HFR8469191.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLS"
     gene            complement(612..872)
                     /gene="spaQ"
                     /locus_tag="ACHX5T_004268"
     CDS             complement(612..872)
                     /gene="spaQ"
                     /locus_tag="ACHX5T_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HFR8469192.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            complement(887..1537)
                     /gene="spa24"
                     /locus_tag="ACHX5T_004269"
                     /gene_synonym="spaP"
     CDS             complement(887..1537)
                     /gene="spa24"
                     /locus_tag="ACHX5T_004269"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HFR8469193.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            complement(1542..>1883)
                     /gene="spa33"
                     /locus_tag="ACHX5T_004270"
                     /gene_synonym="spaO"
     CDS             complement(1542..>1883)
                     /gene="spa33"
                     /locus_tag="ACHX5T_004270"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HFR8469194.1"
                     /translation="LCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLALFNYDDI
                     NVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELVSIEDGY
                     GIEISSWMVKE"
BASE COUNT          743 a          362 c          273 g          505 t
ORIGIN      
        1 cgataataca ccaagtaata cttctgatag taacaatact atcataacag gactggctaa
       61 aataacagcc tgacttgcca ataaagtcag aaatgttaag atatttgaga cacggaaaga
      121 acattgagaa aataacgggc atatattata agacaattgt atggattcta aaataaagac
      181 cataccacca ctgtatagaa atacaactgc agaaaaaaga ttgaaaaatt ttgccaactc
      241 agacgtatca acaccattgg caggatcaat tgaactacta agcgttgccc cacgctggtt
      301 gtcaataatg ctaccaacag catgaaatat ccaaaaggga agagacaagc aaaaagagag
      361 aaggaggcca acaattattt ccttgaacat aaggaaataa acatgttcaa aaacagaaga
      421 acctatgtct accttaccag aagtaattaa tcctgatgca acaagaaaaa tcacaggtat
      481 tctaatagat ggagaaatta taccgttatt taaaaatgga agaaagaaaa ataatggagc
      541 caatctaaaa aaaacgccgt tcaggagtat taaaaacaca tgaatacttt cgaaccagct
      601 tgaaatgtcc atcaaacccc actcttaatt aaaaacatta tttcatgaca aaaagacaat
      661 aaaacctcac cataccatcc agaaagaagt agcaaagata ttgagacacc tataagcttt
      721 ataccaaaag gaagtgtctg ctcttggagt tgagtcactg tctgtaacaa accaatagat
      781 aatccaataa ccgtagctat acctactggc cataaggaaa agataaggat taaataaaga
      841 gccttattac ccatataaac tatatcagac acattacctc catcatctaa gcaggaatat
      901 tgatatattg ttcaatcaag gccttagaca ggatacccca cccatcaagc gcaacaaata
      961 atacaagttt tattggtact gatattgtga ttggactcat catcatcata ccaagtgcaa
     1021 gtaaaatgct tgaaataaca agatcaacaa caacaaatgg caaatataaa taaaaaccta
     1081 ttttgaacgc atctttgatt tcacttagcg catatgcagg caagagggaa aacaatgaat
     1141 aatcattatt ctctgcactc tttaaatcag cattttcttc ttcagacctt tggaaaaaac
     1201 gtgctaactc aagatctgta tgctttttca agtattgctt atattccatc aagccagaat
     1261 cagaaaaacg gacaatatca gatattgtat caaatttttg agggccattt agataatttt
     1321 cataaccagc ttcaataatg ggtttcatca cgaacaaagc cataatcaat gcaataccat
     1381 tgagagtcat atttgatgga acctgctgca aaccaagtgc atttcgtacc attacaaaaa
     1441 caatagaaaa ctttatataa caggtaccag cagcaactaa aaatggtaat agcgtaaaaa
     1501 aagaaagtgt agcgatgagg gacatgtcac tcagcatgag attactcctt taccatccaa
     1561 gaactaatct cgataccata accatcctca atagaaacaa gttccccatg cccaaccaaa
     1621 gaaccattta ctttaatatt tacatgttta actatgtcat cgggaaactt gaataattcg
     1681 ttttctacat acatttttag ttcattgatt gtcatatttt tttctaaaag aataaagtcc
     1741 acttttacat tgatatcatc ataattgaat aatgccaaag aaacttctgt gtgttctgac
     1801 tcaccattac tttctgacag attaattttt gcctcattat tatcattcac aatataatcc
     1861 ccaataataa cttgattaca caa
//
LOCUS       DAZSIO010000348         1861 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZSIO010000348 DAZSIO010000000
VERSION     DAZSIO010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.355"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..362)
                     /locus_tag="ACHX5T_004271"
     CDS             complement(<1..362)
                     /locus_tag="ACHX5T_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8469195.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACHX5T_004272"
     CDS             complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACHX5T_004272"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFR8469196.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          563 a          479 c          378 g          441 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt acgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca
     1741 ttaaaaacaa aaatttctac cgaggagaaa aataccctca aaaaccacct gaataattaa
     1801 ccattattgt taaaacaaca cgttgaataa tcacataaca aactccatta atcattgaaa
     1861 a
//
LOCUS       DAZSIO010000349         1849 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZSIO010000349 DAZSIO010000000
VERSION     DAZSIO010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.356"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..257)
                     /gene="ydfV"
                     /locus_tag="ACHX5T_004273"
     CDS             complement(<1..257)
                     /gene="ydfV"
                     /locus_tag="ACHX5T_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HFR8469197.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMS"
     gene            282..521
                     /gene="relB"
                     /locus_tag="ACHX5T_004274"
     CDS             282..521
                     /gene="relB"
                     /locus_tag="ACHX5T_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HFR8469198.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            521..808
                     /gene="relE"
                     /locus_tag="ACHX5T_004275"
     CDS             521..808
                     /gene="relE"
                     /locus_tag="ACHX5T_004275"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFR8469199.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            880..1035
                     /gene="hokD"
                     /locus_tag="ACHX5T_004276"
     CDS             880..1035
                     /gene="hokD"
                     /locus_tag="ACHX5T_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR8469200.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1253..1504
                     /gene="rem"
                     /locus_tag="ACHX5T_004277"
     CDS             1253..1504
                     /gene="rem"
                     /locus_tag="ACHX5T_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFR8469201.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            1571..1849
                     /locus_tag="ACHX5T_004278"
                     /pseudo
     CDS             1571..1849
                     /locus_tag="ACHX5T_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          507 a          410 c          467 g          465 t
ORIGIN      
        1 gacatgctaa agcaaaaaat agatgaataa gttgagttgt gcatatgtag cctgaccgtc
       61 acaaagtata tggtgtctgt accagtaaga tgatggccgg actctttaaa aaagagctga
      121 cctgcacaat acaggatgga cttagcaatg gctgctcctg gcacaaagcg gacagtgatc
      181 accgttctta cgactacttt ctgacttcct tcgtgacttg ccctaagcat gttgtagtgc
      241 gatacttgta atgacatttg taattacaag aggtgtaaga catgggtagc attaacctgc
      301 gtattgacga tgaacttaaa gcgcgttctt acgccgcgct tgaaaaaatg ggtgtaactc
      361 cttctgaagc gcttcgtctc atgctcgagt atatcgctga caatgaacgc ttgccgttca
      421 aacagacact cctgagtgat gaagatgctg aacttgtgga gatagtgaaa gaacggcttc
      481 gtaatcctaa gccagtacgt gtgacgctgg atgaactctg atggcgtatt ttctggattt
      541 tgacgagcgg gcactaaagg aatggcgaaa gctgggctcg acggtacgtg aacagttgaa
      601 aaagaagctg gttgaagtac ttgagtcacc ccggattgaa gcaaacaagc tccgtggtat
      661 gcctgattgt tacaagatta agctccggtc ttcaggctat cgccttgtat accaggttat
      721 agacgagaaa gttgtcgttt tcgtgatttc tgttgggaaa agagaacgct cggaagtata
      781 tagcgaggcg gtcaaacgca ttctctgaac caaagcatga catctctgtt tcgcaccgaa
      841 ggtgacactt ctgctttgcg ttgacaggag aagcaggcta tgaagcagca aaaggcgatg
      901 ttaatcgccc tgatcgtcat ctgtttaacc gtcatagtga cggcactggt aacgaggaaa
      961 gacctctgcg aggtacgaat ccgaaccggc cagacggagg tcgctgtctt cacagcttac
     1021 gaacctgagg agtaagagac ccggcgagtg agaaatccct cgccacctct gatgtggcag
     1081 gcatcctcaa cgcacccgca cttaacccgc ttcggcggtt ttttgttttt attttcaacg
     1141 catttgaagt tctggatggt gccggaatag aatcaaaaat acttaagtag cgcgcaggga
     1201 taaaagggat ggaccccgaa caggggagtg ctacttatct ggaaggattc tgatgatgaa
     1261 catcgaagaa ctgcgtaaaa ttttttgtga agatggcctc tatgctgtgt gcgttgaaaa
     1321 tggaaatatt gttagtcatt accgcattgt gtgtttgcaa aaaaatgggg ctgcgttaat
     1381 taattttgtg gatgcccgag tgacggacgg atttatcttg cgcgacggtg agtttgtcac
     1441 ttcattacag gcattgaaag agatcggaat aaaagctggc ttttctgctt tttcagaaga
     1501 ataaactcat ctacaatctt gcgcggggct gaactcccgc tgagtaacac cgtgccaccg
     1561 gagaaaaccg atggcacgca acgcaaaata ttacaatcat gataattcga ccgttcttgc
     1621 ccacacgcac gagcggtatt ctcacgcatt taagtcagac tggtaccagc atccctcatg
     1681 cactgaagaa caggccgaat ggcttattca gtgttaccgc aggcgcggat gcgaggttaa
     1741 aaaagccctt agcctcgact accgtcacta gataatctcc gtcaggctcc cttactccga
     1801 acagccaccg cgtccgtccc gcacattcca gcaacggatc tggaggtaa
//
LOCUS       DAZSIO010000350         1832 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZSIO010000350 DAZSIO010000000
VERSION     DAZSIO010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.357"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..180)
                     /locus_tag="ACHX5T_004279"
     CDS             complement(<1..180)
                     /locus_tag="ACHX5T_004279"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469202.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
     gene            complement(321..875)
                     /locus_tag="ACHX5T_004280"
     CDS             complement(321..875)
                     /locus_tag="ACHX5T_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR8469203.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(872..1162)
                     /locus_tag="ACHX5T_004281"
     CDS             complement(872..1162)
                     /locus_tag="ACHX5T_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR8469204.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1162..1761)
                     /locus_tag="ACHX5T_004282"
     CDS             complement(1162..1761)
                     /locus_tag="ACHX5T_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR8469205.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          445 a          496 c          421 g          470 t
ORIGIN      
        1 gttatgggtg atcggggaga gagtttcaac agagatttga gtggtcataa cgataactcc
       61 gtacatttgg aaattatcgc caccgacgac gccaatcgaa ctggtggcga actgtgcagg
      121 gttggcgtaa ccgggtacgg aaaccggcga gcctttcggc tcccccacac agcccgccat
      181 aaatcgcgaa tgtgactgtg caaacgatat gaaaaaagac gcgggcgcgt ctcatatcgc
      241 tccgtaaaca tccgggacgc caatcccgac gccagatttt gctggcgcgt gaggaatata
      301 gccccggata acagattgag tcaacaaacg gtttttagat ccccggaaga gaatgcatca
      361 cgcatcggta gatagagcat atattcagca agatttagcc atacatctac ccgactgcag
      421 cacgtccgga aacaccactc agggtgtgcg tcattcagca attcagccat tttacgctta
      481 gtcatccccc gcccttcata ccgttgccgg aggacactaa tcaatccagg atgctctgcc
      541 agcacctcac ttatgactct atcaatacat aacgcctctg catcagtaca atgcgccagc
      601 caggtctttt gcttgccatt gatcatctct cgcaaaaacg cttccagctc aggtttctct
      661 attcccgctt ttttcatcct gcgcagggct tcattgacgg ctgttttcgt caattttttg
      721 gatgccagca actggttaaa catatttccc gtcttaccgc cgccaatata cgaccagcgc
      781 ccccacatgc gcagttttcc ctgaatccag acactttcca gcgtggtgag acgaaggtgt
      841 tccccgcttt tgcctgtatt tgttgggtaa atcataaata accttccttt ctccagattt
      901 cttgtgtgcg aaaaacacct tctgcatgca tcaggcgtaa ttcttctttg gtgtaatcgc
      961 tggtttttac ccgcccgtcg attaaatcgt ggcatgagct acaggcaatc gctgcctgca
     1021 tatcgtgtgg ttttgtcgct gttccgcacg tccccgccag cctgtaatgc gccagcacag
     1081 aagtttcggg attgtgattg cagtagccag gaattctgac ggtgcacatc tgcccccgcg
     1141 ccgctttacg taaatccacc attacgcaaa ctccagtaac tgcgcggcca cattttcgac
     1201 ttcctccgga gaggaaaatt tacggaacag gatccagttc cacagcacat tcagtacaga
     1261 tttataaacc tgctgaaact cggtttcgtc catgttcgca aatgcgatag attttgccct
     1321 gcgcccacga ctaccatcag gataaatatg ctcggtgtaa aatccggcct gaatggttac
     1381 ccactcgcgg aaagcgtcaa acgactttag caatgccgta tcccgggttc tgcatgtcgc
     1441 aactgtatta aggtattgct ctgcggcatc actcagggct ggagtgtgtt cccgacctac
     1501 tgattcgcac aggtaatcaa cgaagcctga taccagtttt cgttcgcgag gcgtgatcgc
     1561 cccaccgacc ggagtccagt aatcaaatcc cagttgcagg agtttgaaaa aacgcttgtg
     1621 gaatgcgtag ttacgcacac gcttaaagtc tgcgtgtatc cactcaccta ttttgatttg
     1681 atgcaaaaaa tcgcaactct ccggcgtcgc cgggagaagt aatccggaag aggtttgttt
     1741 gaccagttgt atatgcgcca tcgtagttct ccgctggcgc agtagaatgg gtgttcagcc
     1801 cgttatgtag tataccagaa ttaatgccaa ta
//
LOCUS       DAZSIO010000351         1829 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZSIO010000351 DAZSIO010000000
VERSION     DAZSIO010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.358"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..272)
                     /locus_tag="ACHX5T_004283"
     CDS             complement(<1..272)
                     /locus_tag="ACHX5T_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469206.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYS"
     gene            complement(534..>1829)
                     /locus_tag="ACHX5T_004284"
     CDS             complement(534..>1829)
                     /locus_tag="ACHX5T_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR8469207.1"
                     /translation="ADNTTNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGD
                     KSDIIYKVNGLSIIPENFFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFY
                     KQCGVINPQNANTAYFGDTDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASS
                     AYIHKNNKLLYNLLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYI
                     LTLLNSMRNMEGNKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTL
                     DVCNYNSETKVLTSHHGIIRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQ
                     HMDDHLLHLIRSNAIAPTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLG
                     VDQHQKIDNYTMGLNSWKIAPNERGDKKGVPGLSCFQPQ"
BASE COUNT          574 a          333 c          284 g          638 t
ORIGIN      
        1 atactatatt tttctgaatg attcgtttgt atggtaaatg aaattttgtc atcttgaata
       61 gattgttgga aacgttctat ttgccaatca tcaatttttt ccacaacttc accgagcatt
      121 gtcctggcaa tacttgatat atcatcacct ataccatttg caatataccc aaacagttct
      181 ttagtgaagt tggtaacatg ctcttgcacc atatcatttg ttgcgattgt cgttgtactg
      241 ccattagtgc ctgccacagt tcctattttc atcttttacc tcatattatt caccgcgagg
      301 aatacatagt taacatttta tcttatgaca tacacctcat attcgatggg gaaataatta
      361 tattttgcac aaatcattca gccttaaagc ataatatgaa atttcttatc tcatagcaga
      421 gaaagtccct aaatattgta gaggcgtcat cagtacatac ctctacaatc gattaagaaa
      481 acctatgaaa tactggcaaa attaaaaaga gataccacag cactcatgaa gttttattga
      541 ggttgaaaac aactaagccc tgggacacct tttttatcac cacgttcgtt aggtgctatc
      601 ttccaagaat ttagccccat ggtataatta tcaatttttt gatgctggtc tacccctaat
      661 ccataatggc caaatttctg tttagttttt ttgagcgtct gtccacattt gaagatatct
      721 tcaggttttg gtctgaaagc agtagtgtta ttaaaataat tttcatatac aggtgtgggt
      781 gctatagcat tgcttctaat tagatggaga agatgatcat ccatgtgttg tttatgtttt
      841 ttattacata tatttgctaa ttcctcagga tcaattgggt ctttcatctg attaaatgga
      901 acctgcaaag caccgagaca ataacattga ttttcttcat ttcttattat tccgtgatga
      961 ctggttaaaa ctttagtttc tgagttataa ttacaaacat cgagcgtttt aattaagtta
     1021 tacgtatcac cggcagagag tttataattt tctagtcgtg cagtatagtt accattaaaa
     1081 ttgatttcat gatttccagc cagaacgatg acatttttat taatacggct atctttatta
     1141 ccctccatat ttcgcataga atttagcaaa gtaaggatat atttatcacc aaaaatagta
     1201 ctaaatctat cgcctgtatg atccccaagg aagatacaag gggttatcgc atcgtccttt
     1261 aaaaaaacat gttgatgcaa ctcattcaat atcatgtctc tcgtatttaa taaattgtag
     1321 agcaatttat tatttttatg tatgtatgca gatgaagcca tgtcttcgtg ttttaataat
     1381 tcacataata aactccaacc cttttccctg atgcctatat gccctgaaac gagcaatgaa
     1441 taaagtacag cgcctactct tccatctgta tcgccaaaat aagccgtgtt ggcattttgt
     1501 gggttaatca caccacattg tttataaaag tctattttag aaaacacaga tttaacatag
     1561 ctatcaaact tatccttagg aaacgtctct ttagagacat cccaacgcca gtcagcttta
     1621 aaattaccgc taagaagagt aaagaaattt tcaggtatga tagaaagccc attaacttta
     1681 taaattatgt ctgatttatc tccggagaaa ttaataaaat aataaccatc cttacactca
     1741 atcttaactg taatgggaga cgataatttt atcacatggt tattgtctgg tataattgta
     1801 aagataaacg tgttagtagt attatcggc
//
LOCUS       DAZSIO010000352         1823 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZSIO010000352 DAZSIO010000000
VERSION     DAZSIO010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.359"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            191..793
                     /gene="fimB"
                     /locus_tag="ACHX5T_004285"
                     /pseudo
     CDS             191..793
                     /gene="fimB"
                     /locus_tag="ACHX5T_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            1271..>1823
                     /gene="fimE"
                     /locus_tag="ACHX5T_004286"
     CDS             1271..>1823
                     /gene="fimE"
                     /locus_tag="ACHX5T_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFR8469208.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWE"
BASE COUNT          514 a          337 c          389 g          583 t
ORIGIN      
        1 catggcgttt gtatggcaaa gttattataa gtaactatcg ctactccatt taagttcgct
       61 cagaagaact ggtccactta cgtaagttat taagcaaacg ttcgctttta taaacataat
      121 caggataaaa atgttggatt attgctaacc cagcacagct agtgcgcgtc tgtaattata
      181 agggaaaacg atgaggaata aggctgataa caaaaaaagg aacttcctta cccatagtga
      241 aatcgagtca ctccttaaag cagcaaatac cgggcctcat gcaacacgta attattgtct
      301 gattttgctt tgttttattc atggtttccg ggcgagtgaa atttgtcgat tgaggatttc
      361 ggatattgat cttaaagcaa agtgtatata tatccatcga ttaaaaaaag gcttttcaac
      421 aacgcacccg ctattgaaca aagaagttca ggctttaaaa aactggttga gtatccgtac
      481 ttcttatccg catgctgaga gcgagtgggt atttttatca cgtaagggga atccgctttc
      541 tcggcaacag ttttaccata ttatctcgac ttctggtggt aatgccgggt tgtcactgga
      601 gattcatccg cacatgttac gctattcgtg tggttttgct ttggcgaata tgggaataga
      661 tacgcgactt atctaggatt atcttgggca tcgcaatatt cgtcatactg tctggtatac
      721 cgccagcaat gcagggcgtt tttacggcat ctgggataga gccagaggac gacagcgtca
      781 tgctgtttta tagagatagt tgagatacca gggatggtgt taaaaactac attattctta
      841 cgatatatca aaaatgatta accatttatt aattgatgtt attgtaagtg tgattcttaa
      901 ttatcataat aaacattaag ttaaccatac ccatacaaaa tctaatgatt tatgttcttc
      961 aaaataaata aacaaaatca ttcataagtt tacacatcac ttaaattctc ctgtttccac
     1021 acttttttct ttatttttta agtaactgga agttaatcct ctgtaatcta ttgttatatt
     1081 gaatcaaatc aatgaaaata gatgttgtca catcagtgat attttatttt tgtatgatgt
     1141 ttaatgtaat tgactgatag ccacataact tcgtgtgtgg ttatcttttt atctattggg
     1201 ctaattttga ccgattgagg tttcctatag gtattcattc aaatatatct cagttaggag
     1261 tactactatt gtgagtaaac gtcgttatct taccggtaaa gaagttcagg ccatgatgca
     1321 ggcggtttgt tacggggcaa cgggagccag agattattgt cttattctgt tggcatatcg
     1381 gcatgggatg cgtattagtg aactgcttga tctgcattat caggaccttg accttaatga
     1441 aggtcgaata aatattcgcc gactgaagaa cggattttct accgttcacc cgttacgttt
     1501 tgatgagcgt gaagccgtgg aacgctggac ccaggaacgt gctaactgga aaggcgctga
     1561 ccggaccgac gctatattta tttctcgccg cgggagtcgg ctttctcgcc agcaggccta
     1621 tcgcattatt cgcgatgccg gtattgaagc tggaaccgta acgcagactc atcctcatat
     1681 gttaaggcat gcttgcggtt atgaactggc ggagcgtggt gcagataccc gtttaattca
     1741 ggattatctc gggcatcgaa atattcgcca tactgtgcgt tataccgcca gtaatgctgc
     1801 tcgttttgcc ggattatggg aaa
//
LOCUS       DAZSIO010000353         1817 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZSIO010000353 DAZSIO010000000
VERSION     DAZSIO010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.360"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..1411
                     /gene="tnaA"
                     /locus_tag="ACHX5T_004287"
     CDS             <1..1411
                     /gene="tnaA"
                     /locus_tag="ACHX5T_004287"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFR8469209.1"
                     /translation="NFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSEDV
                     FIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQGRG
                     AEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFDTG
                     VRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAKKY
                     DIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPMGG
                     LLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIA
                     QVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIRAV
                     EIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGL
                     TFTYEPKVLRHFTAKLKEV"
     gene            1502..>1817
                     /locus_tag="ACHX5T_004288"
     CDS             1502..>1817
                     /locus_tag="ACHX5T_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR8469210.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYI"
BASE COUNT          482 a          428 c          445 g          462 t
ORIGIN      
        1 aaactttaaa catctccctg aaccgttccg cattcgtgtt attgagccag taaaacgtac
       61 tactcgcgct tatcgtgaag aagcaattat taaatccggt atgaacccgt tcctgctgga
      121 tagcgaagat gtgtttatcg atttactgac cgacagcggc accggggcgg taacccaaag
      181 tatgcaggca gcgatgatgc gcggcgacga agcctacagc ggcagccgca gctactatgc
      241 gttagccgag tcagtgaaaa atatctttgg ttatcaatat actattccga ctcaccaggg
      301 ccgtggcgca gagcaaatct atattccggt actgattaaa aaacgcgagc aggaaaaagg
      361 cctggatcgc agcaaaatgg tggcattctc taactatttc tttgatacca cgcagggcca
      421 tagccagatt aacggctgta ccgtgcgtaa cgtctatatc aaagaagcct tcgatacggg
      481 cgtgcgttac gactttaaag gcaactttga cctcgaagga ttagaacgcg gtattgaaga
      541 agttggcccg aataacgtgc cgtatatcgt tgcaaccatc accagtaact ctgcaggtgg
      601 tcagccggtt tcactggcaa acttaaaagc gatgtacagc atcgcgaaga aatacgatat
      661 tccggtggta atggactccg cacgctttgc tgaaaacgcc tatttcatca agcagcgtga
      721 agcagaatac aaagactgga ccatcgagca gatcacccgc gaaacctaca aatatgccga
      781 tatgctggcg atgtccgcca agaaagatgc gatggtgccg atgggcggct tgctgtgcat
      841 gaaagacgac agcttctttg atgtgtacac cgagtgcaga accctttgcg tggtgcagga
      901 aggcttcccg acatatggcg gcttggaagg cggcgcgatg gagcgtctgg cggtaggtct
      961 gtatgacggc atgaatctcg actggctggc ttatcgtatc gcgcaggtac agtatctggt
     1021 cgatggtctg gaagagattg gcgttgtctg ccagcaggcg ggcggtcacg cggcattcgt
     1081 tgatgccggt aaactgctgc cgcatatccc ggcagaccag ttcccggcac aggcgctggc
     1141 gtgcgagctg tataaagtcg ccggtatccg tgcggtagaa attggctctt tcctgttagg
     1201 ccgcgatccg aaaaccggta aacaactgcc atgcccggct gaactgctgc gtttaaccat
     1261 tccgcgcgca acatatactc aaacacatat ggacttcatt attgaagcct ttaaacatgt
     1321 aaaagagaac gcggcgaata ttaaaggatt aacctttacc tacgaaccaa aagtattgcg
     1381 tcacttcacc gcaaaactga aagaagttta attaatacta cagagtggct ataaggatgt
     1441 tagccactct cttaccctac atcctcaata acaaaaatag ccttcctcta aaggtggcat
     1501 catgactgat caagctgaaa aaaagcactc tgcattttgg ggtgttatgg ttatagcagg
     1561 tacagtaatt ggtggaggta tgtttgcttt acctgttgat cttgccggtg cctggttttt
     1621 ctggggtgcc tttatcctta tcattgcctg gttttcaatg cttcattccg ggttattgtt
     1681 attagaagca aatttaaatt atcctgtcgg ctccagtttt aacaccatca ccaaagattt
     1741 aataggtaac acctggaaca ttatcagcgg tattaccgtt gccttcgttc tctatatcct
     1801 cacttatgcc tatatct
//
LOCUS       DAZSIO010000354         1809 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZSIO010000354 DAZSIO010000000
VERSION     DAZSIO010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.361"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            173..1198
                     /locus_tag="ACHX5T_004289"
     CDS             173..1198
                     /locus_tag="ACHX5T_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR8469211.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="ACHX5T_004290"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="ACHX5T_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          421 c          462 g          505 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctggct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtgag
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgcc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DAZSIO010000355         1804 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZSIO010000355 DAZSIO010000000
VERSION     DAZSIO010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.362"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            308..1498
                     /locus_tag="ACHX5T_004291"
     CDS             308..1498
                     /locus_tag="ACHX5T_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HFR8469212.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
BASE COUNT          492 a          380 c          425 g          507 t
ORIGIN      
        1 gtaccaccga tagtatcgat atcaaatcag cctgcggccg cgataacttt cgtaacattc
       61 tgccagcggc tttgttgaat aaatcaggtt caagataatc gtctctgcat caggttagag
      121 tttcaggcaa tacatcctct ttctggcata cctgccactc tcgcaccatg gtcatagcct
      181 ttacgatctg catactcacc agaccagagc ccgctccctt ttaggctgag gaatccccag
      241 aaaagaagac aggcatagag ttttgtgatc tactgattgc gctaacaaat tcaatgagat
      301 cacctctatg cctgcccgta aagtatgcca gacttttttc cgtaatgctt tagcttccac
      361 gcatcagtat cgacaaaatg ccattattga ttctgctgct gctttagtcg gtggcgcatc
      421 tctttctctt accagcattg gccggcattt acccggtcct gctcgcgtga aagataaaat
      481 aaaacgcgtt gactgtctgt tgggtaataa acgacttcat aatgatatcc ctctgatatt
      541 caaaaatatt acatccatga tgacaaataa actgtcgtgg tgtgttatcg ctgttgactg
      601 gagcggttac ccttttcagg aatatcatgt ccttcgtgcc agcctcctct gcgacggtcg
      661 ctctatcccc ctgatgagtc aggtcttccc gtcaaaaaaa aataacgagg cggtggaaat
      721 cgcttttctg gatgcgcttt ccggggccat ttctccccgg actcgtgttg ttattgttac
      781 tgatgctggt tttcaaagtg catggttccg tcatatcaaa tcacagggat gggattttat
      841 tggtcgtatc aggggggtag tgaagttcag gctggatagt gacaaagata agtggctgga
      901 cataaaaatg tgcaggggga gttcagaggc aaaatacctg gggacgggaa ccctggcccg
      961 gaaaaaacgc tcgcagtgtg aaggacattt ttatctttat aaacattcac ctaaaggcag
     1021 aaaaagcagg cgtgccagag ggaggccggg cttaccaaca acggaaaaag aacaaaaagc
     1081 cgcaggcaga gaaccatggt taatattcag caatacagct gagtttaatg ccaaaaaaat
     1141 catgaaactg tacagtcgaa gaatgcagat agagcaaaac tttcgggatg aaaaaagtga
     1201 acgctttggt tttggccttc gggcgagcag aagtcggagg ggagagcgtt ttctggtatt
     1261 aagtctactg gttacactgg ccagtatcgt actctggcta cttggttatc attttgaaaa
     1321 taaaggattt catttaaagt atcaggctaa tagtctcaaa aaacgaagag tattgtcttt
     1381 tctgacgctg gctgaaaacg tcctgagatt taaccctgaa cttctacgac gagcacaacc
     1441 tgaaaagata ttgtgccggt tggccagcac ttaccgaagt atggtgctgg cttactgatg
     1501 aaaattttct ggggatccct cagcctttcg gggaggaatg agggctctga tattcttttg
     1561 ccgtagttca tcgtgaaacc gtcggatgtc atttggcctg agtgacccat gcctcccaaa
     1621 agcagagtcg ctagtgttat gtaattaggc ggttaaactt caattctcag tgttaagttt
     1681 gggtaaacgc ttgtaaatgg tattgctaat tttttaattg actaatatta gaaacggtta
     1741 gtgtgattgt atatagatat ttgagtgtaa ggtatttaaa ggggcattca gcccctccgt
     1801 ctat
//
LOCUS       DAZSIO010000356         1799 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZSIO010000356 DAZSIO010000000
VERSION     DAZSIO010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.363"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            18..1799
                     /locus_tag="ACHX5T_004292"
                     /pseudo
     CDS             18..1799
                     /locus_tag="ACHX5T_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
BASE COUNT          606 a          343 c          333 g          517 t
ORIGIN      
        1 aacaatgaga tatttaaatg attactcgta ttcctcgtag ttctttctct gcaaatatta
       61 ataatacagc ccagacaaat gaacaccaaa ccctgagtga attgttttat aaagaactcg
      121 aggataaatt ttctggcaag gagctggcga cgcctctatt aaaaagcttc tcagagaact
      181 gtagacaaaa tgttcgtcat atctttagca acaaggattt tgtcattaaa ttttccacgt
      241 ccgtcttaca agctgataag aaagaaatta cgatgattaa taaaaacgaa aacacgacac
      301 tcactcaaac cattgctaca atatttgaag aatacctaat ggaaatttta cctcaacgct
      361 cagacactct tgataaacaa gaattaaacc aaaaatcaga tagaaaagaa aaagaattcc
      421 caagaattaa acttaatggt caatgttatt tcccggggcg ccccaaaacc gtatagtata
      481 ccgacacatt gctgcacaat atattaatga tatttatcag aatgttgatt acaaacccca
      541 tcaagatgat tactcttcag ctgaaaaatt tctcactcac ttcaacaaaa aatgcaaaaa
      601 ccagactttg gcgttgattt ccagccgtcc tgaggggcgt tgcgttgctg cctgcggtga
      661 tttcgggcta gttatgaaag catattttga caagatggaa tcaaatggca tcagtgttat
      721 ggcagccata ttactggtgg ataaccatgc tttgacggtc cggctaagaa taaagaacac
      781 aactgaagga tgtacccatt acgtggtttc ggtttatgat cctaatgtaa ctaacgataa
      841 aataagaatt atgagcgaaa gcaaagagga tattaaacac tattctctga tggattttat
      901 gaatgtagat tatagcctcc tgaaatggtc aaatgatcat gttattaacc aatctgttgc
      961 aataattcca gcacttccga aagaacagct attgatgtta aaaggaactg tggatgaaat
     1021 aacccctcca ttatcaccag caacgatgaa tttgctaatg gcaattggtc agaatcacca
     1081 acttacgcaa ctgatgattc agctccagaa aatgccagaa ctacatagaa cagaaatgtt
     1141 gactgcctat aatagtatta acctccccgg tttatatttg gctataaatt atggcaatgc
     1201 ggatatcgtt gagactattt tcaattcatt gtcagaaaca ggatatgaag ggttactctc
     1261 gaaaaaaaat ctcatgcata ttctggaggc aaaagataaa aatggttttt ctggattatt
     1321 tttagcgata tcacgtaagg ataaaaatgt tgtaacctcg attctgaacg tcttacctaa
     1381 actggccgca acacatcatt tagataatga acaggtgtat aaattcctga gtgccaaaaa
     1441 tagaacgtcc agccatgttt tataccatgt tatggcgaat ggtgatgccg acatgctgaa
     1501 aatttttttg gtcgcgttac ctttgttaat tcgcacatgt catttgacta aagaacaggt
     1561 actcgatctc ctgaaggcaa aggattttta tggttgccca agactatacc tggcgatgca
     1621 aaatggacat agcgatatcg tgaaagttat tctcgaagca ttgccctgtc tggcccagga
     1681 aattaacatt tcagcttccg atattgtaga tcttctgacc gctaaaagtc ttgcgcgcga
     1741 cacgggtttg tttatggcca tgcagcgcgg acatatgaac gttattaata ctattttta
//
LOCUS       DAZSIO010000357         1782 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZSIO010000357 DAZSIO010000000
VERSION     DAZSIO010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.364"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(78..839)
                     /gene="kduD"
                     /locus_tag="ACHX5T_004293"
     CDS             complement(78..839)
                     /gene="kduD"
                     /locus_tag="ACHX5T_004293"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR8469213.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(869..1705)
                     /gene="kduI"
                     /locus_tag="ACHX5T_004294"
     CDS             complement(869..1705)
                     /gene="kduI"
                     /locus_tag="ACHX5T_004294"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR8469214.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
BASE COUNT          467 a          476 c          402 g          437 t
ORIGIN      
        1 catgatatgg attatgatga tctagagata taaaaaaccc tgccatgcgg cagggtcata
       61 aaagtaagag gaatgaatta acgcgccagc caaccgccat ccacagcaat ggtataacca
      121 tttacataat ctgaagcgct ggaggcaaga aacactaccg gccccatcag gtcactcggc
      181 agtccccaac ggccagctgg aatgcgatcg agaatttccg cgctacgttg ttcatctgcc
      241 cgcagttgtt gggtattgtt ggtcgccatg taacccggag ctatcgcatt aacattaatg
      301 ttgtgtttag cccattcgtt tgccatcaat cgcgtcacac ccatcacgcc gctttttgat
      361 gcggtataag aaggcacacg gatcccgccc tggaaggaga gcattgacgc gatattgata
      421 atcttgccgc cattgccttg cgcgataaag tgtttcgccg ctgcctgaga catgaagaat
      481 acgctcttga tattcaggtt catgacatcg tcccagtctt tttcgctgaa ctcgagagcg
      541 tcttcgcggc gaatcaatcc ggcgttattc accaggatat caatatgacc aaattcagct
      601 accgcgcgat ccagcagtcc aggaatgcca tcaatctttc gcagatcggc ggtcaggctt
      661 aaaaaacgac gccccagcgc cgtgacctgc tcgatggttt cagtcggttc aacgatgtta
      721 atgccaacaa tgtcacagcc cgcttgcgcc agccccaacg ccatcccctg gcccagccca
      781 gtatcacaac cagtgacgac cgcaacttta ccttggagag aaaatgcact taaaatcata
      841 acaacacctt attcgtttat gcccacaact agcgcaaatc tttaacgccc acatggtcca
      901 tatcattaaa gacctggttt tcaccgacca tgccccagat aaaggtataa gctttggttc
      961 cgacgccgga atggatcgac cagctcgggg agatcaccgc ctgctcgtta tgcatcacaa
     1021 tgtgacgcgt ttcttgcggc tgccccatca tgtggaaaac gcaggcgtca tcatccatat
     1081 tgaaatagaa ataaacttcc atccggcgct cgtgggtgtg acacggcatg gtgttccaca
     1141 agttacctgg agccagctcc gtcagcccca tactcaattg acaggtttcc agtacatccg
     1201 ggacaaaata tttgttaatc gtgcgacggt tactggtgag gttatcgcct aacgtgactg
     1261 gagatacttc gtccggtgtg acttttttgg tgggatacgt cgtatgcgcg ggtgcgcaat
     1321 tgtaataaaa cttcgccgga gtggcggtat caatactggc aaagacaact tcttttgcac
     1381 ctttaccaac atacagggcg tcgcggtgac cgatttcata gcattgacca tcgacagtaa
     1441 tcgtaccggc accgccaata ttgataacac ctaactcgcg acgttcaagg aaatagctta
     1501 cgccgagttg tttaccaact tccccgccaa cggaaaccgt tttagttatc ggcataatgc
     1561 cgccaacaat aattcggtca atgtggctgt aaaccatggt gtactcatcg gcgacaaata
     1621 ccttttcaac caaaaattca ttgcgcagcc cttgggtatc cagcgttttt gcgtgcgcac
     1681 cgtggatgct ctgtcttacg tccacatcaa cctccgataa tcagtgaagc gtgaaaatag
     1741 aaaacgatag tgaaacactg tttcgttttc tctataaagg ga
//
LOCUS       DAZSIO010000358         1759 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZSIO010000358 DAZSIO010000000
VERSION     DAZSIO010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.365"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(1..263)
                     /locus_tag="ACHX5T_004295"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="ACHX5T_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1016)
                     /gene="ospI"
                     /locus_tag="ACHX5T_004296"
     CDS             complement(378..1016)
                     /gene="ospI"
                     /locus_tag="ACHX5T_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HFR8469215.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1163..1348)
                     /locus_tag="ACHX5T_004297"
     CDS             complement(1163..1348)
                     /locus_tag="ACHX5T_004297"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR8469216.1"
                     /translation="MLPLSVNLMSSGASLAARLQQHWLWYSYNTKTGGVLAYTFGPRN
                     DETCRELLVLFTPFCIY"
     gene            complement(1373..1645)
                     /locus_tag="ACHX5T_004298"
     CDS             complement(1373..1645)
                     /locus_tag="ACHX5T_004298"
                     /inference="COORDINATES: protein motif:HMM:NF024169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /protein_id="HFR8469217.1"
                     /translation="MATVTVHCPRCHSDEVYRHGRSCSRHERFRCRSCKRVFQLTYSY
                     EARKPGVKEQIVEMAHNGAGGRDTARTLKIGINTVIRTLKSSRPGG"
BASE COUNT          502 a          433 c          319 g          505 t
ORIGIN      
        1 agcaagcgct gtaactcttt tatttgtttt atagcggcag ccagttctga ggcaggaacg
       61 acatcctctc cggcagcaac agcagtgaga cttccttcca gatattattt tttccactta
      121 agcaacaggc tgggctgaat accatgcaaa cgcgccacat gggacaccgt catgccaggc
      181 tccattgtct gctgaataat ggcaattttt tcctgaggag ttttacgttt acgaagctcc
      241 agccccagca aagttccagt catctcaaaa ttagaactag tgttagacat atatccaagc
      301 ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg ctcaaatcta
      361 tgtacaggct ctcagcatca gcaaagcctc ttacttttcc aactactact gttagcatag
      421 tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc atctacaaat
      481 gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt ttgaacatcc
      541 aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc ctgttcaata
      601 gaaatcatta ctacacttcc ttttgaagta ttttgtaaaa tattttctgc aatacgctca
      661 cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc aatttgctct
      721 aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac gggagcctct
      781 cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga acagttacca
      841 tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga atctttatcc
      901 atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt tacatttccc
      961 tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt aatcatattg
     1021 tatcctgaca gtatatatac tcactatatt ttcttcataa caaatatccc tctgatatga
     1081 caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc agctattcgt gtctaaaaag
     1141 tgatccactt accaccaacg gcctaatata tacagaacgg agtaaacagg actaacagtt
     1201 cccgacaggt ctcatcattc cgtggcccga aagtgtaagc caacacaccg ccagttttag
     1261 tgttgtacga ataccagagc cagtgttgtt gcagccttgc tgccaacgaa gcgccactgc
     1321 tcatcaagtt cacagataag ggcaacatca gcgtgagcga ctggcgcaga tgttatccgc
     1381 cggggcggga gctttttaat gtccgtatga cggtattgat gccgattttc agtgtcctgg
     1441 cggtatcacg gccccctgcg ccgttatgtg ccatttcaac gatctgctcc ttaacgccgg
     1501 gttttctggc ttcataagag taagtgagct ggaaaacacg tttacatgag cgacaacgga
     1561 aacgctcatg ccgggaacaa ctacggccat gtcgataaac ttcatcggaa tgacagcgcg
     1621 gacaatgaac agtaacggta gccatggaaa accttaaata ccagtagatt accagaaact
     1681 tctgtcgatt gaagacatca cccgaagttt gaattcattc gaatactttt tacgaggttc
     1741 ttttcgccag tcctgtaat
//
LOCUS       DAZSIO010000359         1751 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZSIO010000359 DAZSIO010000000
VERSION     DAZSIO010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.366"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            311..505
                     /locus_tag="ACHX5T_004299"
     CDS             311..505
                     /locus_tag="ACHX5T_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469218.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            866..994
                     /locus_tag="ACHX5T_004300"
     CDS             866..994
                     /locus_tag="ACHX5T_004300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469219.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(1304..1640)
                     /locus_tag="ACHX5T_004301"
                     /pseudo
     CDS             complement(1304..1640)
                     /locus_tag="ACHX5T_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          524 a          389 c          375 g          463 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat
       61 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
      121 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
      181 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
      241 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
      301 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
      361 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
      421 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
      481 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
      541 gcatcgttat cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct
      601 ttgtttgccg gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg
      661 acattgtaat aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat
      721 cgttgacaat atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct
      781 gaagcatccg acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg
      841 gaactttgac aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg
      901 gaaaagtggt acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag
      961 cttgtcagaa aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag
     1021 tgcccggcca gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc
     1081 aggctatagg caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac
     1141 ggtctgaata acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc
     1201 gagcgctgat tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa
     1261 atatgaaaag atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc
     1321 gtttaatatt cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt
     1381 acgaccacag tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata
     1441 agcagttgct tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg
     1501 atttccacat tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc
     1561 tgaagttctt tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa
     1621 cccactgtac tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact
     1681 ctatacagga taattagagg tcagtttgat tattcacaat tcatcatcag cattttctat
     1741 ttctgacgaa a
//
LOCUS       DAZSIO010000360         1748 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZSIO010000360 DAZSIO010000000
VERSION     DAZSIO010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.367"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            453..1301
                     /locus_tag="ACHX5T_004302"
     CDS             453..1301
                     /locus_tag="ACHX5T_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR8469220.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
     gene            1318..1599
                     /locus_tag="ACHX5T_004303"
                     /pseudo
     CDS             1318..1599
                     /locus_tag="ACHX5T_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
     gene            1635..>1748
                     /locus_tag="ACHX5T_004304"
     CDS             1635..>1748
                     /locus_tag="ACHX5T_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004998486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469221.1"
                     /translation="MAGIRSTLIKEKNMNTNYEATVATTDNIVHEVYLEGKH"
BASE COUNT          499 a          398 c          411 g          440 t
ORIGIN      
        1 gcattatata gttctactct ggcgagatac gttggcatat tcatttcctt actggttgtg
       61 tgagaacttc agtaaagata ccaccaaagc ccggaagtgg tgaaataaaa ccgggcacaa
      121 cacgaaggcg catttccggt atccataaag agtcggtctt gtctgtgaaa tttaaatggt
      181 gggagtgcgc ttccggttgt aaataacgac attgctgtgt gaagtaccag ttggcggcat
      241 cggtttaatt gctggctgat gtccgccctt tttaaagtga attttgtgat gcggtgaatg
      301 cggctatgcg cacgcggcac agttaaaagt catgttagtc cttattggtt tgggtgggaa
      361 agccaactgt aattgttaac tggttaacgt cacctggagg caccaggcac cgcatcaaca
      421 aagttcactt cggtgatgaa aggaaagaga aaatgttgaa tgtagctatt gaaaaccaga
      481 acgggtggaa ttatagtgca cctgcacctc ataaaacggg tgccgggatt gctaccccga
      541 cgatgactac tgcgcataac cgcgctcagg cggttttttt atgcgtcaag cacagccaca
      601 ttcagattat ggtggggcgt gcagggcagc cgcaaggctg gccggtttcg gtagtcaccg
      661 gttgtagcaa ccctgtgcgt ctcaccaccc atgagattgc tacctctggt ggtgagtcat
      721 ttaaactgac tatcgaggct gccatcatgg ctactatcct taccctttct caccctgacg
      781 caaccatcga aaatggtcgc gctgtcacta cgtctgttgc ggttgcggag ttcttccgga
      841 aaatgcacaa aaacgttatt cagaagatcg aaactctcga atgctcgccg gagtttaacc
      901 ggcttaattt taagcccgtc acctataccg acgccaaagg cgaaaaacgc ccaatgtacc
      961 aaatcaccaa aaacggcttc gttttcctgg tgatgggctt cactggcaaa aaagccgccg
     1021 cattcaagga agcctacatt gctgagttcg accgcatgga ggccgaactg cgccagaata
     1081 acaccccacc tgcagacaag atgattccgg gtgatggtcg cactctggtt gttcacttcg
     1141 acaaattcgg caatgtcgaa ttcaccgaaa ccgttcctga tggcgcactg gtctgtaccc
     1201 tggagacttt ccggttttat ctggaaaaac agggatggac tcttgttaac cggggcgcaa
     1261 ttaaaaatat gactgtggaa caattacttt ctttaaaata aaaatggagc tacggatatg
     1321 aaccctatgc ttgattattg ttttacctct gcacctcagc agaccataac acacaccgga
     1381 atcaaaaatt tgttatggat aatcaaaaaa aacctccagt agaaaggtga tcccgattta
     1441 attccctgtg cttatggcct gtgtgacaga caagtaagcc ctgataaggc ttacactgct
     1501 gaacttattt acatgcacgg aaccatgatg aggaaaaaga aacgcaaata ttgtagtgaa
     1561 atctgcgccg aaaaagacca gatggcacac gaactttaat taactgacta ttcgaaactg
     1621 aatttatgcc agcaatggca gggattcgct caaccttaat taaggagaaa aacatgaaca
     1681 ccaattatga agccactgtt gcaactactg ataacattgt tcacgaggtt tacctggaag
     1741 gaaagcat
//
LOCUS       DAZSIO010000361         1744 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZSIO010000361 DAZSIO010000000
VERSION     DAZSIO010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.368"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..353)
                     /locus_tag="ACHX5T_004305"
     CDS             complement(<1..353)
                     /locus_tag="ACHX5T_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR8469222.1"
                     /translation="MNRHTQIRQSVLACLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMA"
     gene            complement(350..940)
                     /locus_tag="ACHX5T_004306"
     CDS             complement(350..940)
                     /locus_tag="ACHX5T_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR8469223.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            complement(927..1298)
                     /locus_tag="ACHX5T_004307"
     CDS             complement(927..1298)
                     /locus_tag="ACHX5T_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR8469224.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            complement(1310..1711)
                     /gene="gpFI"
                     /locus_tag="ACHX5T_004308"
     CDS             complement(1310..1711)
                     /gene="gpFI"
                     /locus_tag="ACHX5T_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR8469225.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
BASE COUNT          393 a          527 c          407 g          417 t
ORIGIN      
        1 gccatctcat tatcacgttg atagttaaaa ccgagtgggt tcagggtgtc gatgagtccg
       61 gaaagtgccg gtacatcatt cagggccggg aaaatggtgc tctccatcca catatccagc
      121 tctgaatccg gtgcctgtgc ccggatgaag acggcaatat gcagaacagc ctgccagtca
      181 tcttcatccg tcatttttcc ggtgtactga gcatcactca gccacaccgc cacggcaggc
      241 agttcctgcg catcaataaa tgccggaagc ccgtcaaaaa acgtggcgct gtctccacac
      301 tgttcccgaa ggcatgccag cacgctctga cggatttgtg tgtgtcggtt catttactca
      361 gatgtaacct cagttgttgt ttcagggcat accccagctg tttcggcatt tccgcagcaa
      421 tgatgcggtc gcgggcatct tcaaatgcct gtgtcagcgg tccggacagc gggattttca
      481 ctacctccac aggaagacga ttttttctta gcctgccctg atggtcatgc cctgttgcaa
      541 aacgcgcttc aggaagacac ctcagaacat gccagcggcc attcgccagt tgctgaacaa
      601 atgccccccg gaaaaagtat tttcccactc tcagtccttc accagcacgc cgccgtgttg
      661 tgttcagttt gatggcgggc ggattgcccc ggttaacgcg gatcctggcc gtcatttttc
      721 ctgacggact ggctttaaac acccggacac gctgacgtac cagtttcagg gggatccctt
      781 tcacctggtt atcgcccgca acggtattcc cggcaacctg ccgggtggca accgagaccg
      841 ctttctgtgc cacacggttt atcgcccatg cgctggcctg tggcaccata cgggtatcaa
      901 ggctgttcag attgcggatg gcattctcaa gccccttcat aatatcgtcc tgcccttacg
      961 tttcccggca gcccctcacg ccgcaaccgg atataactac atccgccatc atcaggggtg
     1021 atacgatcca cacagaaaga ctcacgacca atcatcagcg aatcattgcg acacagccgt
     1081 aaaatatctg ctgttttcac aaacagggat ggtaaagagt cttcaatacg cacaccgccg
     1141 gccaccagag aaatattttc aggatcatca aaaaccccgg aaatcatgat cccttttagc
     1201 cggcctgacg taatgaccgc tctaatcccc atattgcgaa taatggcctt gtctgcgaga
     1261 tcaagtgcaa catcaaacag attatcgaaa tcagccacgc tgcctcccgt tattgcattc
     1321 tggccaggcc acgttctgtc attccggctg ccacaccggc aaagacacgg aacgccattc
     1381 ccggcggcac aaatgccaga ggttcatccc gcgtggcatg aagtgcatcg gtatgcagcg
     1441 tcaccagtgc cacgactgta accagatcat ccatattctg aatcacggtc tccggctgcg
     1501 ctgatatcac ctcattttca tgtccggtca gcgcattttc cgggtgggca gacttatccc
     1561 ggatgtcagt gtcatccata ttggcaagtt cctcctccag ctcagccaca cgctgcgcaa
     1621 tatctgctgc gcttcccgag atatccggct cacgtcccag cgatgctgcc agttctctca
     1681 gacgtttcag gttatcgtct ttctttgcca tatctgttcc tgtccgaata aaaaacggga
     1741 gccc
//
LOCUS       DAZSIO010000362         1732 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZSIO010000362 DAZSIO010000000
VERSION     DAZSIO010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.369"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..143
                     /locus_tag="ACHX5T_004309"
     CDS             <1..143
                     /locus_tag="ACHX5T_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR8469226.1"
                     /translation="AEVELATLTWVDWYNNRRLPERLGHIPPAEAEKAYYASIGNDDL
                     SA"
     gene            127..486
                     /locus_tag="ACHX5T_004310"
     CDS             127..486
                     /locus_tag="ACHX5T_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469227.1"
                     /translation="MICQPEFTDKTLSRKPGAVQKVNETDAEYLVRKFDELEAKCAAQ
                     ENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLEN
                     VNITHWAVMPKAPEAGA"
     gene            486..791
                     /gene="yfdT"
                     /locus_tag="ACHX5T_004311"
     CDS             486..791
                     /gene="yfdT"
                     /locus_tag="ACHX5T_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdT"
                     /protein_id="HFR8469228.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            707..1141
                     /locus_tag="ACHX5T_004312"
     CDS             707..1141
                     /locus_tag="ACHX5T_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8469229.1"
                     /translation="MGYDSRLDRLAATSWYPFLNNVTARGEIMEPYSLTLDEACHFLK
                     ISRPTAINWIRTGRLQATRKDPTKNKSPYLTTRQACIAALQSPLHTVQVSAGDGITEE
                     RKCHSSAEVKYGTPVSHCRTVKDLNSLLEQRTKGRRQNSMTS"
     gene            1063..>1732
                     /locus_tag="ACHX5T_004313"
     CDS             1063..>1732
                     /locus_tag="ACHX5T_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8469230.1"
                     /translation="MPNGKRFKQSLGTKDKRQATELHDKLKAEAWRVSKLGEIPDITF
                     EEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGIQLRDITESKIYSAMQKMTNRRHEE
                     NWKLRAESCRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIKV
                     PQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQINMQRRV
                     AWINP"
BASE COUNT          568 a          379 c          428 g          357 t
ORIGIN      
        1 gtgcagaagt ggaactggcc acactaacgt gggtggactg gtataacaat cgacgattgc
       61 cggaaaggct gggccacatc cctccggcag aagcagaaaa agcttattat gcttccatcg
      121 gaaacgatga tctgtcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg
      181 gttcagaaag taaatgaaac agatgctgaa tacctggtgc gtaaattcga tgagcttgaa
      241 gctaaatgtg cagcacagga aaacaaagta ataccagtgt caactgaact gccaccagca
      301 aatgaaagtg ttttgttatt cgatgctaac ggagaaggct ggctaattgg ctggcgttct
      361 ctctggtaca cctggggaca aaaagaaacc ggagaatggc agtggacatt tcaggtcggg
      421 gaccttgaaa acgtcaatat cactcactgg gcagtaatgc caaaagcacc ggaggctgga
      481 gcataatgac cacttttacc gacaaagaac tgattaaaga aattaaagag cgtatcagca
      541 gccttgacgt gcgagacgat attgagcgcc gtgcttatga aatcgcactc ctatctctgg
      601 aagtagaacc agatgaacgc gaagcttatg aattattcat ggaaaagcgt tttggtgact
      661 tagtagatcg tcggagagca aaaaacggcg ataacgaata catggcatgg gatatgactc
      721 tcggttggat cgtctggcag caacgagctg gtatccattt ctcaacaatg tcacagcaag
      781 aggtgaaata atggagccat acagcctcac actcgatgag gcctgtcatt ttctcaagat
      841 atccagaccg actgccatta actggatacg cacagggcgt cttcaggcaa cacgcaaaga
      901 tcccactaag aataaatctc cttacctcac aacacgacaa gcctgcattg cggctcttca
      961 gtctccgctg catactgtcc aggtgagcgc gggtgatggc ataacagagg aaagaaaatg
     1021 tcactcttcc gcagaggtga aatatggtac gccagtttca cattgccgaa cggtaaaaga
     1081 tttaaacagt ctcttggaac aaaggacaaa aggcaggcga cagaactcca tgacaagcta
     1141 aaggctgaag catggcgggt cagcaaactt ggtgaaatac ctgatataac gttcgaggaa
     1201 gcgtgtgtca ggtggcttga agagaaagca cataaaaaat cactggacga tgacaaaagc
     1261 cggatcggat tctggcttca acatttcgca ggaatacaac taagagacat tactgaatca
     1321 aaaatttatt cagcaatgca gaaaatgacg aaccggcgtc atgaggaaaa ctggaaactc
     1381 agggcagaat catgcagaaa aaaagggaaa cctgttccag aatacacgcc aaaaccagcg
     1441 tccgttgcaa cgaaggctac gcatctttca tttataaagg ccctactaag agccgcagag
     1501 cgtgaatgga aaatgctgga taaggcacca attattaaag tgcctcaacc aaagaataaa
     1561 cggatccgct ggctggagcc ccatgaagca caaaggctga ttgatgaatg tccggagcca
     1621 ttaaagtctg ttgttgaatt tgcactggca acaggcttaa gacgctcgaa catcatcaac
     1681 cttgaatggc aacaaataaa tatgcagcgc cgggtggcat ggataaaccc gg
//
LOCUS       DAZSIO010000363         1725 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZSIO010000363 DAZSIO010000000
VERSION     DAZSIO010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.370"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..251)
                     /locus_tag="ACHX5T_004314"
     CDS             complement(<1..251)
                     /locus_tag="ACHX5T_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469231.1"
                     /translation="MNAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWF"
     gene            669..1667
                     /locus_tag="ACHX5T_004315"
                     /pseudo
     CDS             669..1667
                     /locus_tag="ACHX5T_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
BASE COUNT          452 a          353 c          348 g          572 t
ORIGIN      
        1 taaaaccata ttttctccat ctgaagacta tgttcattta ccctccagtg accatgttgc
       61 aggaaataga tgatatgcat gccaaaatcg ttatctattt gcgaatcatt atccagctta
      121 tcaatttcaa ctatctttga acgatgtgtt ttatcaacct tgctatggtg ttgatgatta
      181 ttaactttat ctgaatgctt atctttatgg tttgaaggga tgtacttaaa tatattgtca
      241 attgcgttca taaataaccc gcgatatttt tgacctgtta gtaaaaaata aatgatacat
      301 gtctgaaaga catatttacg acgatataaa acaataatac ttatatgttc gctgatttta
      361 agcaccgaga ctatccggtc ttttactaaa taatggaaaa caatccatcc ctggaagaca
      421 attgtaaaaa aaaacaacag aataagaatt aatcttagga taaatattta tttatcatgg
      481 ccttttgacc gctaaaaaat ggcactcggc acagtaaacc ccgctatagt tatgctatgc
      541 atttgtcttt aatgatatct cgtcgttata ttaacaggct gaaattatca ttaatgcaca
      601 tttcaatatt agcagggata cctgaggagt ttaaagtgaa catatatatc gggtagcttt
      661 tcaaattaat ccctttgatt ataggcttaa tttgcatcgc gttaggtggc tttgtgctgg
      721 aaagttcagg gcaacgtcac gggtcatgtg ctgatttctc tggcggccat atgcctggca
      781 ttattcacta ccgcatttat tatcatttcg caactcacgc gcggcgttaa tacgttttac
      841 aatacattgt tccccattat tggctatgcg gggtcaatta tcaccatgat atggggttgg
      901 gcactgttag caggtaatga tgtgatggca gacgagtttg ttgccggcca tgttattttt
      961 ggcgttggta tgattgccgc atgtgtatcg acggtggcag cgtcgtccgg tcactttctg
     1021 ctcattccca aaaatgcagc ggggagcaag agcgacggaa caccggtaca ggcttattct
     1081 tcattaatcg gtaattgcct cattgccgtt cccgttttac tcaccctgct cggtttcatt
     1141 tggtctatta cgctgttaca tagtgccgac ataactccgc attatgtcgc cttgggttaa
     1201 ccgcaatctg tgcctgtcta attggcctag tggccacaat cgtccatcaa acacgtaata
     1261 cgttctcatc caaagagcac tggctgtggt gttattgggt tatttttctc ggctcagtca
     1321 cggtgctgca ggggatatac gtcttagtca gttccgatgc aagcgcccga ctggctcccg
     1381 gcattattct catttgcctc ggaatgatct gttacagcat attctcaaaa gtctggctac
     1441 tggcactggt ctggagacgt acctgttcgt tagccaacag aataccgatg ataccggtat
     1501 ttacctgtct gttttgcttg tttttgtcat cattcctcgc ggaaatcgca caaactgagc
     1561 tggactactt tattccatct cgggttctgg ttggattagg cgcagtatgt tttacattgt
     1621 tctctattgt ttcaatatta gaggccggtt ctgcgaaaaa aaattaaaat ttacataatc
     1681 ccagcgttat tcatatctta acgctggttt tttacgaact tagtg
//
LOCUS       DAZSIO010000364         1717 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZSIO010000364 DAZSIO010000000
VERSION     DAZSIO010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.371"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(110..577)
                     /locus_tag="ACHX5T_004316"
     CDS             complement(110..577)
                     /locus_tag="ACHX5T_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFR8469232.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="ACHX5T_004317"
     CDS             complement(643..882)
                     /locus_tag="ACHX5T_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HFR8469233.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="ACHX5T_004318"
     CDS             complement(1017..1622)
                     /locus_tag="ACHX5T_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8469234.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          549 a          378 c          305 g          485 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata tttttgggat
      961 gtggctataa ttatctattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacattaag ttaagca
//
LOCUS       DAZSIO010000365         1645 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZSIO010000365 DAZSIO010000000
VERSION     DAZSIO010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.372"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(72..148)
                     /locus_tag="ACHX5T_004319"
     tRNA            complement(72..148)
                     /locus_tag="ACHX5T_004319"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(112..114),aa:Arg,seq:tct)
     gene            complement(150..225)
                     /locus_tag="ACHX5T_004320"
     tRNA            complement(150..225)
                     /locus_tag="ACHX5T_004320"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(190..192),aa:Ile,seq:cat)
     gene            complement(402..1091)
                     /locus_tag="ACHX5T_004321"
     CDS             complement(402..1091)
                     /locus_tag="ACHX5T_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFR8469235.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
     gene            complement(1088..1447)
                     /locus_tag="ACHX5T_004322"
     CDS             complement(1088..1447)
                     /locus_tag="ACHX5T_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR8469236.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGGRLGIKITELECA"
     gene            complement(1447..1635)
                     /locus_tag="ACHX5T_004323"
     CDS             complement(1447..1635)
                     /locus_tag="ACHX5T_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469237.1"
                     /translation="MPFYFRKECPLNSGYLNMVPALRELMQAKHRAQKAVDELVDCVA
                     ELETRVGKFEKLVQEVLR"
BASE COUNT          394 a          462 c          352 g          437 t
ORIGIN      
        1 gactaccgga atcgaactgg tgacctactg attacaagtc agttgctctg cctgctgagc
       61 taagtcggcg ctggcccacc accgaggact cgaacctcgg accgtcaact tagaaggttg
      121 atgctctatc cggatgagct agtggtggtt ggtggccctt gctggacttg aaccagcgac
      181 ctggcgatta tgagtcgctc gctctcacca ctgagctaaa gggccggagg cagaataata
      241 accatatgtc atcacatctg caaactcatc tgaccaccag cacgtttaac gtcctgtgcc
      301 atttttcagg cataaaaaaa ccgctcggcg gcgggttttg cttactttac catcgcgtac
      361 aaaatcggca aagtatcaga tttgcatgag atatatgcct tttaatctac ttttgcaata
      421 ctttgctttg aaaatgccgc tttttgttct gaacgtgttc tcatcacaaa caataaagcc
      481 tcactatcca gtcggtgaaa aatgtgtttc attacaaccc agtgaccagt aaatgttttg
      541 gaccagtttt tggttgttac tcccgccagt aatgccagct cctgatattc ataaccttcc
      601 ccaccaaaaa gttctgcttt taccgcctgc gccgccagcc agattaattt tttcaggcgt
      661 tcctgtgttt tctctgcaat tttcctagtg ccagaatgag ccttaaactc ttcccacgcc
      721 cagtgggtta tggtgatctg atactcccag caaatacttc cgctgtaaca ccacaacaac
      781 caggctttat agtgctcttc aagagacaga acagcccgcc gccacgatga tgtcgaaaac
      841 gcaacctgac tgaccagagg aattgacgcc cccttcgcca gtgattgttt tcccgggatt
      901 ggtggattat cccgcgttat catttttccg gtcacctcat cgcggtaccg gattttttta
      961 cgcctgtaac gccctgtatc gaacatggca ttctcctgcc aggcttcaag ctgacctttt
     1021 gtcgccccac tcaaatcagc ggtggcgata atgagctgct cacgaacaaa ctgtaaatac
     1081 tggttattca tgcacactcc agctctgtga tttttatccc caaccgacca ccaggaacag
     1141 gcagtccgcg cacaatattg gtttcatcaa actgctcgtc gtcgataagc aaccccgcat
     1201 gtgtcagtgc atccagtggt gctttcagga tattgtccag gtcacgacgc cgcttatccg
     1261 gtggctctgc aataatcttt atcgccagtc ttccggacag gcttaatttc agtcgctgct
     1321 gacggacaat aagcgccaca tcccggcgat aacgctcacc ggcttttgat acaaaatatg
     1381 tgctgccacg acgtcgccag taagtattca ccgtcggcgg ataaggtaaa ataaactcat
     1441 ggcgcatcag cgcagcacct cctgcaccag cttttcaaac tttccgactc tggtttccag
     1501 ctctgccaca cagtccacca gctcatctac tgctttctgt gcgcgatgct tcgcctgcat
     1561 cagttcccga agcgcgggta ccatattgag gtagcctgag tttaacggac actccttcct
     1621 gaaatagaat ggcatcagaa ggagc
//
LOCUS       DAZSIO010000366         1611 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZSIO010000366 DAZSIO010000000
VERSION     DAZSIO010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.373"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            25..>1611
                     /locus_tag="ACHX5T_004324"
     CDS             25..>1611
                     /locus_tag="ACHX5T_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HFR8469238.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETY"
BASE COUNT          606 a          280 c          289 g          436 t
ORIGIN      
        1 aattattcac atggaaaata catcatgtac aaatcattat ttactattat cattctactg
       61 tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat aactttcatt
      121 gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat gaaactcgtt
      181 cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc aatttctgct
      241 cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa aaaattagaa
      301 actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga cggtaatggc
      361 actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc tgaatataca
      421 aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa acgtctggca
      481 atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg gaaagtcacc
      541 ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc atttgaagga
      601 tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa atcctggttt
      661 gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag agatggaaaa
      721 tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt ttctaatggt
      781 cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag taaacgtcat
      841 tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga tgggaaaatc
      901 aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga atattatcct
      961 gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg aaacgatacc
     1021 agttactacc aatccggaaa aataagctct atacacaata gaaattcccg tggtcagtat
     1081 gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc cgtctataaa
     1141 gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga agaggagaaa
     1201 cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa aaatggtaat
     1261 cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga agagtggtac
     1321 gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga cggcattgcc
     1381 aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa tggcgtagag
     1441 aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga aaccctgtac
     1501 gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg taaactatta
     1561 acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta t
//
LOCUS       DAZSIO010000367         1603 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZSIO010000367 DAZSIO010000000
VERSION     DAZSIO010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.374"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(335..481)
                     /locus_tag="ACHX5T_004325"
                     /pseudo
     CDS             complement(335..481)
                     /locus_tag="ACHX5T_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            535..717
                     /locus_tag="ACHX5T_004326"
     CDS             535..717
                     /locus_tag="ACHX5T_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR8469239.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(818..1147)
                     /locus_tag="ACHX5T_004327"
     CDS             complement(818..1147)
                     /locus_tag="ACHX5T_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR8469240.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1319..>1603)
                     /locus_tag="ACHX5T_004328"
     CDS             complement(1319..>1603)
                     /locus_tag="ACHX5T_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469241.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          436 a          368 c          377 g          422 t
ORIGIN      
        1 atagcaatgt ctgtcgcaca acaaccacaa tgtcacaaat aaaaaatgag aataaccagt
       61 tccctgaagg cgacgtagcc cggtacaaga gtaacccacg ttagcgcttg gcgacggcgg
      121 aacaaagctg attcgcgacg taagaaagtg gcaggctcat atgacggacc tctgtaatgc
      181 ggtatccaac ccgcgaatat cagcatgatt caccgtcgga tttactgcta cgtcgccctc
      241 agggaattaa acctaaaaag aaaatcgtca aaattagaga ttccggtttg acacggaaag
      301 tcatatcagc gtcaacggag caccgtattg acgcttattt attggtgaga actacgttcc
      361 atggcaggag ttcgtcaaca cggttggagg gccattcagg cagtatgctc agaatatggc
      421 gcagatacgc ttccggatcg ataccgttca gacggcaggt gcctgctttt ccggcaacaa
      481 gcaggaatct cagcgctgaa gcaggaatta gaggtaaaaa cgccctaccg ggccatgaat
      541 cacccggtca ttggagtagt aaccaaagca gatttagcca gcatggaaca aatatctttg
      601 gtgaaaagtt ggttacggga agctggagcg cacaacgtgt tagtaaccag tgcagttaat
      661 aacaatggag ttacagaact ttttgccttg ctgcatacag aagaaggctg ttgttaattt
      721 cgattggttc tcagatgaga atgatgacgg tgatacacag ataagaatca atgagcaatg
      781 agggttgccg ggcaaccctc attgaataaa acgggaatta cttcgcttcg ccgtttttca
      841 tttcgcccat agctttcagc tttttggaga tatcgcggcg ttctttggag agctcggcat
      901 ttttgatgat gtaatcgtca acgcgatctt catagtcacc tttcatgctg gcgatgatgc
      961 cctggattgc ttcaacgctc atccctggct tgatgtaatc gctcaggttg tccagcaaca
     1021 ggacgcgctt ctggttgtca cggatctttt tctcaacgtc ctgaatttca cgttgcagtt
     1081 tgttcttacg acggaacaga cgaacaaatt ccagtacgtc ctggaatgaa ggcttggtag
     1141 tttccatttt tatacccctg ataatgtgag agtcggattc gtttaagcaa ccgctattcg
     1201 ttagggccaa cattactcat agctgccgca gatgacaatg cttttatccc ttttcactat
     1261 catacccttt atccttgctg aatcgaagca gcagcaagat gattctgaag ttcaggaatt
     1321 atttgcgcaa ggcccggcaa atcagattcg atagcaactc aagctgatgt gccgtttcca
     1381 tgttcagcca cacataacca tagattggtg tttccacgac tttcaggtca tggtggttat
     1441 cgagcaactg acgcagctct tccacagcat cgttcaattt ttccgtattg gcaaaaaccg
     1501 gctgcggatt accttcgtac agcgcatgaa caaggctcag cagtatttgc tgcatcatgt
     1561 gctgggtatc acaaagtttt tgcgcgttca ataacacgaa atg
//
LOCUS       DAZSIO010000368         1602 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZSIO010000368 DAZSIO010000000
VERSION     DAZSIO010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.375"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            7..657
                     /locus_tag="ACHX5T_004329"
     CDS             7..657
                     /locus_tag="ACHX5T_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469242.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            629..1063
                     /locus_tag="ACHX5T_004330"
     CDS             629..1063
                     /locus_tag="ACHX5T_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR8469243.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
BASE COUNT          451 a          326 c          377 g          448 t
ORIGIN      
        1 taatccatgc atcgtataga cacgaaaacc gcgaagaagg ataagttcgg cgcgggtaag
       61 aacggtttta cccgtggtaa cccccagact ggcacgcctg ccaccgatct ggatgatgac
      121 tactttgaca tgttgcagga ggagctttgc agcgtggtgg aggcatccgg tgccagcctg
      181 gagaaggggc ggcacgatca gttgcttacc gcacttcgcg cgctgctgtt aagccgcaag
      241 aatccgtttg gcgatatcaa atcggatggc acggtgaaaa cggctctcga aaaccttggt
      301 ttgggagaag cagctaaaag gaatgtaggt acaggggcga atcagatacc tgatatgagc
      361 ctgttcgcgt caattaatac cgtaacggct gctgcgcaaa aatttccgtc tggattaatt
      421 ttacagtgcg gtcagttgaa tggtgccccg aatgtatctt caacatacgg gatgaggttc
      481 ccgatgacgt tctcaagagt aattgctgtc gtagttacat tgaacgttac tggcgcggct
      541 gggcagccga ctgtatcggc gacaagtgtc cagaacactg gatttaatat tactgtgtcg
      601 cccggttcag gatacggttc atctgctgat gcgtattaca ttgcaatggg atattaacaa
      661 aatgtcatat ttttattctg catcgacaaa cggattttat tcgactgaat ttcacggcac
      721 caatattcct gatgatgcag tggaaatctc ggaatcagag tggaaaacac tgattaatgc
      781 acagagtgta acaaaaatga ttacctgtgg tgagaacggt catcctgtca ttgttgaccg
      841 tccttctcca acaccagaac aattagcctt aataaatgat gaaaagaaat ctgcactgat
      901 agcagaggca acgaatgtaa tagctccgct tcaggatgcg gttgatttag gtatggcaac
      961 agatgatgaa acgaaactgt tactggcatg gaaaaaatat cgggtgctgc ttatgcgtgt
     1021 taatgtagta aaacccgagt ggcctatgca tccaaataaa tagacttgtt aatctaggat
     1081 aattgttgga ataatactcg tttttcagta ggttcaggta gcctgggtat aatgctcact
     1141 tctacaatga tttcaactta ttttatttaa ttgatattac gaaacttaac tcatacataa
     1201 acaatatgac tcaatactgt cctaaatagc tgcgcagaaa agtgctccac tttcgctcag
     1261 cccttgctgg gcgaagcatc aggaaggcgt tctcagctag gttgtgttat atctggcttg
     1321 agttttgttg cccatacgca acacattacc catctgtaaa cgacaagtgt ggcagaatta
     1381 ggtgcagcta tttagactat atcgacgcag taacgataat agatatcgaa actgtactag
     1441 atattagcta cttatggtta ccggggggag tcttttgagc actggtgtta tcaattggct
     1501 tcaaataatt cgccaataaa ttatattgcg atagctcatc accttccagg ttgtttatag
     1561 ccagtttatc agcgtcacca cctactgttt atgcttttat tt
//
LOCUS       DAZSIO010000369         1591 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZSIO010000369 DAZSIO010000000
VERSION     DAZSIO010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.376"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..1154
                     /locus_tag="ACHX5T_004331"
     CDS             <1..1154
                     /locus_tag="ACHX5T_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469244.1"
                     /translation="EDMCERMKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKY
                     SAKDVGPDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNV
                     PKNNKPKTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACE
                     AHSHRSVALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFN
                     QSDISVRNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVM
                     GCKSAKDRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKD
                     LFDIRVLHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
BASE COUNT          486 a          295 c          367 g          443 t
ORIGIN      
        1 ttgaggatat gtgtgaaaga atgaaaggtg gcatctctat gtttaaccac accacaaaaa
       61 cagctgataa tatagagcat aatttgcagc taagttttct cgtgggttca cgtccgtcga
      121 tcaccaataa atactcagca aaagatgtag gtccagatat acttgcattg cccgtcaacg
      181 tatatcatgc ggattttaaa attaaaatca ataatgcagt ggagaaggct tcatcaattg
      241 tgttatgtgc tgtgcaaaaa aatctccatg cagcaaggaa ggttcttagc gggataatag
      301 gaaatgtgcc aaaaaataat aaaccaaaaa caattatttt tgatttgcgt tttcttagtg
      361 caattaagca gaaagttttt ttgggcagtg aaaaaccgtt gtttgtaaag cataccgcaa
      421 tgatgaggga ggcatgtaag gaattaccgc agtcagtgga atatgttcct ttagcctgtg
      481 aagcgcatag ccatcgcagc gtcgcccttg ccaaaaccgt ggctgcgccc atcatggcga
      541 caggtgtggg cttggcatta atgtatgcaa catcatcagg accgtattat gcgcaaagtc
      601 tttctggttc gccggatatc gagatgataa aaaatcgcat ggctgaattg tttaaccagt
      661 cggatatttc tgtgcgcaac aaatatagcc cggcttttga taaatggaat gaactggtag
      721 ataaattaaa tcacgaaaga aatacagttc cgtttgcgtg tttgaccacc attttagcga
      781 cggcgataaa tgaaactctt gagggggata cgaatgttgc cgttgtgatg gggtgtaaat
      841 ctgcgaaaga tcgcacgata tccattgtcc ttggcaactc gatgttgcaa acactccttg
      901 aaaaacgcct cgccgatagt ggagaaatag aaaatctttt tgatcaacag ggatatttta
      961 gttgcgattc attaacaccc gaagaattaa tgatgttaaa agaccttttt gatattcgag
     1021 ttcttcatgt ctctaacaaa ttcaatgttg gattgcaagg taatatcaac actgatgttt
     1081 tacaagattc gttttttaaa aatgtcgatt tcattcatga atataactcg tataccgtag
     1141 ggatgggagt ctgacgagta aaactcatta ggattatcag aattcggaca atgcattgca
     1201 aaaatgacat acaaagagag atgtgtgcag gtacttagcc gcgaggggca tggcgaagcc
     1261 atacagaaag taccagtatt cagaactcca gtgaaagcgg catagtggcc gcctgctcag
     1321 gagttttgct atgacccgta atcgtgctga acgtcgccac cctatgatgc gattaaaaaa
     1381 actcatcgtc gcgctgatga ttgacgtcaa tgctatcgga tgatgccggg ggtgttccgg
     1441 aaggaattcc ttgtttcccg aactgttacc aacaaaatta taacgatgag caagtaaaga
     1501 ccggttggtg gcagtactga ccggtctgtg atgaaagatt aatttcacct taaatcctgg
     1561 tgctgttagg atgctgacgt ttttcggcta c
//
LOCUS       DAZSIO010000370         1589 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZSIO010000370 DAZSIO010000000
VERSION     DAZSIO010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.377"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..847)
                     /gene="icsP"
                     /locus_tag="ACHX5T_004332"
     CDS             complement(<1..847)
                     /gene="icsP"
                     /locus_tag="ACHX5T_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HFR8469245.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTS"
BASE COUNT          466 a          325 c          264 g          534 t
ORIGIN      
        1 gagatgtatc acctttttta ttagaaatgt aatttctgga gccttctata taaaattttg
       61 cactgggggt gacatagtat ccaatattcg cagcaacaga gcagtaattt tggtttttta
      121 tttcatcttt aaaaattgtc tcagtctgat agtgtttatc tgtatcagat gaaagaaccc
      181 aaccactata tttcagttct gctccaaact caaaattctc atggcggtaa ttagcagtta
      241 atccaatata aggtattttg aagagctgtt tgtaacctat tgttctttca ccactaggat
      301 gtgccccttt tttattcctg ctccccccgt tttcactata aatataactc cctcccattg
      361 cattaaaact gtaacgactc tcttggtaac ctgctattag tccaagtcga taatccaaat
      421 tatttaataa ccaaccttta agattcagct cgaattcgtt agcatcacgg agatgcgtat
      481 tgggatgcca gctctggtcg gtccatacct gaggggtatt ggaattgttc cagtctttat
      541 caaccatgct tgctttctgg ttacctaaag tagtccaacc ggaaactccg aaagacactt
      601 ttggaattag cttccaatcg atgccacctc taacaatagt cgcattactg tatttccagt
      661 ccagttgact aatttttctc cccccttcct tgggatgata aacgcgttct tttgttttgc
      721 cactcagaga tcctagacta atatccgtac tgatgttgtc cggtatgaaa agtggatagt
      781 tagtggtagc atgtgtagta aagatcgcag gtacacaaag tgcaagtaca aagaatttta
      841 atttcatcga gaactctact tttttggttg aaatgtccat gatcttctta ctttataaga
      901 taaacgtcaa tgccaagcat tattttaatt aaatatttgt ttatgttacc aagaacatat
      961 cttttattat aatatttatt gaacttttgt aaaaataaaa aaatacctca ttgtgcgaat
     1021 aaagtaacgg gggccataga tttctctacc taccttttgg tgttcatacc tgaggctgat
     1081 agtttcagga ttccgcgaca attctactca ccttcttgtt ctctcgccaa tatgaaatca
     1141 atcagaaaag cgttccataa gaacaacgat aagacaataa attgtcatca cggctgcaat
     1201 agccaacgtg cctttcagta ccaacatgac aacctcctgt atttgacgta acgtgtgctg
     1261 ataaatatgt atttgtatct caactgactc aatacaacac ttaagtatag tttttcagat
     1321 tttgtttttc ccgtgaaata ttatggaaat ggcagaagca atacattgaa ttccgattcg
     1381 agtgaatacg gctagcgggc acctcacttt agcactgaag ccatcaactt ttatttgact
     1441 tatttcaatg gaaagctttc tcactatcag tacaggtaat tcccatcctt ttaaagtcga
     1501 gagtcagact gcgactaata cagttaaagt gaccaaactc agttatcctc tacacatttt
     1561 atctcatgtt tacttggaca attcattgg
//
LOCUS       DAZSIO010000371         1581 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZSIO010000371 DAZSIO010000000
VERSION     DAZSIO010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.378"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACHX5T_004333"
     CDS             complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACHX5T_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFR8469246.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLK"
     gene            complement(1269..>1581)
                     /locus_tag="ACHX5T_004334"
     CDS             complement(1269..>1581)
                     /locus_tag="ACHX5T_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HFR8469247.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
BASE COUNT          482 a          400 c          406 g          293 t
ORIGIN      
        1 tttcaaaggg aggatgatga gatcgggaat aaaaacaaat acgactaata atgaatataa
       61 aaccaggacg taagggaaaa cgcctctgac tgcatcatcg aatttgagtt ttgccacccc
      121 tgaaataacg ttaagtacat taccgatagg cggtgtaatt aagccgattg agcagttaat
      181 gataaacatg acaccgaaat aaatcggatc gattcctgcc tctttaacta aaggcattaa
      241 taccggggta agaattaata ccgtcggcgt taaatccatg accatgccga caatcaggat
      301 agccaccata atgacgataa acagcagacg cggtgaatcg accaacggtt gcagtaaatc
      361 agaaaccatc atcggcagtt cagcaatggt aatcagccag gcggaaactt gtgccgaggc
      421 caccagaaac ataaccactg aggtggtttt cgccgcgcca atgagtacat gccaaagtgt
      481 ggcgaaggtc atttcacggt aaataactgt ggcgacaaac agcgcataga aagcagcaac
      541 ggccccggct tcggtagggg taaacagacc ggagcgaaac ccgccaataa tgatgactgg
      601 aagaaacagc gcccagatac cggagacaaa agagtgccag atttcctgca tcgttgcttt
      661 ttgctggcgg ggtaaattaa ggcgactggc ctgccgccac caggtaagca tcagcgttgc
      721 gcccatcatt atgccagggg caatgcccgc cataaacagc ttactgatgg ataatccgct
      781 ggaaacaccg aagataataa acggaatgga agggggaata attggcgcga taatgccgcc
      841 agaggcaatc agccccgccg cccggttgac cgggtagttg gcgctgcgca tcatcggcac
      901 cagtagagcg gcaacggcgg cggtatccgc aacggcagaa ccagaaaggc tcgccataat
      961 catagctgcc agcacgccga cgtagcccag cccgcctggt ttatgcccca ccagtttcat
     1021 cggcaggtca acaatgcgtt ttaacaagcc gcccgcattc atgatttcac ccgccagcac
     1081 aaagaacgga atcgccagga gggagaagct atcggctccg ttcaccagcg tttgtgccat
     1141 gatctggaca tcaaacatgt ccagccagaa cattaacgcc gccccgcaca acaacagtgc
     1201 ccaggcaata ggcaaaccga tagcaatacc acccaacaga cagcccagaa aaatcagcac
     1261 agccatgatt aagctccttg cggtggcgac gttagagaat tgctacgcgt gatgagttga
     1321 tataaatgac gcagttcaaa gaatgcgata acgaggctgg tgggcagaca agcggcatac
     1381 atcaggccga tgggcaaacc gaggatcggt gaataatcgc tccagtcctg aattgttttt
     1441 agcgttgcgc cccaggccag tgcgccacaa ataaataaga ttaaggaatg ggtaaccaga
     1501 gcgactcgtc gttgccatgc gggggagagt ttctccacca gaaaggtgac ctgaacgtgg
     1561 gcgttatcca taaaagccac a
//
LOCUS       DAZSIO010000372         1542 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZSIO010000372 DAZSIO010000000
VERSION     DAZSIO010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.379"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(1..487)
                     /locus_tag="ACHX5T_004335"
                     /pseudo
     CDS             complement(1..487)
                     /locus_tag="ACHX5T_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(509..1049)
                     /gene="yhaB"
                     /locus_tag="ACHX5T_004336"
                     /pseudo
     CDS             complement(509..1049)
                     /gene="yhaB"
                     /locus_tag="ACHX5T_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
     gene            complement(1305..1523)
                     /gene="tdcR"
                     /locus_tag="ACHX5T_004337"
     CDS             complement(1305..1523)
                     /gene="tdcR"
                     /locus_tag="ACHX5T_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFR8469248.1"
                     /translation="MSKFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNT
                     GIDHLEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          486 a          267 c          281 g          508 t
ORIGIN      
        1 aatcgacatt acgtaagtcc agagaggata gatcaactat atctccagtc ccacaactaa
       61 agattacgtt caacctcctg atttcattgt aaatatcttt taatgggcaa ttaacattga
      121 tattgcctgt agatatttcc tagtttttta acggttcatt aatggtaagt tcgcaggtat
      181 tgtccgatcg ttgaataatt tgggtgttat aacctaaaaa atccagacga agagacgagt
      241 cattataaag aattacattt tgtaagtacg ctaattcttt ttgaaaatca ccagtgataa
      301 tgcattttcc acttggttgt aacgcgttaa gtaaagtgac catttgaata gaggtctgtt
      361 ttagtttttc tatacctggt gctagtgctt cgaggttatt tcctatgggg ctttcaggaa
      421 ggtcattcat ttttcgacgg actaaatcac tactaatatc attaccaatt gatgaaacag
      481 ggaacattat tttatccttt ataaataatc aataaaaatc attattaaaa tgatcgggtg
      541 aaaaaaagag aatttgccaa caatctgaag taatgcaggt tttattaaag tgcctagggg
      601 gtgttaagat ggcttctttt tgggtgaact ttgcgcaata cttagaaatc gtctccttaa
      661 tacattccag ctctttttta ttgacggctg agcaagtaca ttgttcacca aaaggtgcaa
      721 aagtgcctac atctaatgtt tgtatgatat aatcatcatg ttctcctgta gaaatatcta
      781 tgatgaatgg actcatattt tccggttgga ttcggaatca tgacagaagg aacatcgcct
      841 tgatagaaat tcaatgcagt aattgcagat aatactctcc ggagttcatt acttatactg
      901 taaattttat taacgtcatt ggtggagaca atttctgcaa actttctttt taccgtccaa
      961 taatcaatat ttctattgtg attgttaacc actgcgtgca ttggcgggat tgaagggtga
     1021 aaaatatgtg caattgggaa gcctttcatt gatgcctgcc ttgtcgtcat gacaaacaat
     1081 aatagatgct tattttcctc gcacgacact tacgcactat caaaaaacgc tcatttaccc
     1141 ggcgttacag taaacgaagc aacattctgt gtcctacgct gatgtaaagc acagtgaaaa
     1201 tcccccttgt tgacttcgtc tgaccttatc gcggcgacca gcaattattc gttttgtgat
     1261 cctcaacaca aaatgctatt cgtcgcctat gaggggagaa gaatttatgg aaaacgattg
     1321 cattcacaaa atgacgtcct gtcagaagag aaaaagttga cgttaatcaa ttgttcgaga
     1381 tgatcaatac ctgtattcga aattttaaaa tattgataaa ataaatgttt gttctccaac
     1441 aaatagcgtg aaaaaatgtg acgtgccgca gtatttatcg ctgaaaatgg tttatttata
     1501 ataaaatttg agaacttgct cattaaattt aactcaaatt tt
//
LOCUS       DAZSIO010000373         1532 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZSIO010000373 DAZSIO010000000
VERSION     DAZSIO010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.380"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..291
                     /locus_tag="ACHX5T_004338"
                     /pseudo
     CDS             1..291
                     /locus_tag="ACHX5T_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            293..659
                     /locus_tag="ACHX5T_004339"
                     /pseudo
     CDS             293..659
                     /locus_tag="ACHX5T_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            662..1060
                     /locus_tag="ACHX5T_004340"
                     /pseudo
     CDS             662..1060
                     /locus_tag="ACHX5T_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
BASE COUNT          379 a          380 c          461 g          312 t
ORIGIN      
        1 tcaacgtatt accactgtca gcaacagcga catcatcatg ataaacgcag tgcccgtgcg
       61 cagcgcgatg actggctgaa gaaagagata ctgcgcgtat acgatgagaa tcatcaggta
      121 tacgctgtgc gtaaagtctg gcatcagttg ttacgggaag gtatcagagt ggccagatgc
      181 actgtggcac gtctcatggc ggttatgggg cttgccggtg ttctccgggg taaaaaggtc
      241 cacactaccg tcagccggaa agccgttgcc gcaggcgacc gcgtaaaccg tcatcaaggc
      301 aatgtgccgc gtactccggg tggcccgcag cggctggtat acgtggtgtc agcggcggac
      361 aaggataaac acacgtccgc agttccgtca gcactgcgac agcgttgtcc gcaaggcttt
      421 tacccagtcc agcgttacgg tgccccacgc ctgacggatg aactgtgtgc tctggttacc
      481 actttaacgt aaaaaccgtg gcagccagtc tgcgccgtca ggaactgagc gcaaaagcct
      541 cccagaagtt cagcccgatc agttaccgtg cacacggcct gcctgtatca gaaaatctgc
      601 tgacgcagga tttttacgcc agtggcccga accagaagtg ggcgggagac atcacgtact
      661 attacagctc accgactgcg gggaaacacg gggcgccggg ttattgaagg tgacctgtcc
      721 agctacttcg acacagtaca tcaccggctg ctgatgaaag ccgtctgccg caggatcagt
      781 gatacacgtt ttatgagact gctgtggaaa acgccgtgtt gatgttggtc tcttccgggc
      841 ggtcagtgaa agtatgccac agggcggtgt gatctcgccg ttgctgtcga acatcatact
      901 gaatgagttc gaccagtatc tgaataaacg ctacctgagt ggaaaagcca gaaaggatcg
      961 ctggtactgg aatcacagta tccagcgagg ccgaagtact gctgtcaaag aaaactggca
     1021 atggaaacct gccgttgctt actgttgcta tgccgatgac tgtgtgccac gaaggcgcgt
     1081 cctggggacg tgagtccctg tgcagccatg cagcacaaga gatgagggaa ggccccttgg
     1141 tgtcggcgtg caggcgcagg caccaaacca tccactgctg ctgcatttaa gagatgtggt
     1201 ggtgagcgag atgggaaaag gtcggacgtg agccgatcag gtatggttcg aagagacgaa
     1261 cgaaagtgaa ccgctgatga tgtgtcgaaa gcatatagat gatgtcaaaa caatggtgga
     1321 gtcgttcctg tgggatatgt ttactgcctg cgcggcatcc ggcataaaag cggcgtgaag
     1381 tcgattcagg ctcttgtggg gaacgtggac ctgtcacccc gatgtgaagg gagaagcgca
     1441 agtggaagcc ccatgagcgc cagagtacca atgcggggta cagggacgga gcaccaagcg
     1501 attgcgcagt aaacatgctt gacaacttaa tt
//
LOCUS       DAZSIO010000374         1527 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZSIO010000374 DAZSIO010000000
VERSION     DAZSIO010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.381"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..132
                     /locus_tag="ACHX5T_004341"
     CDS             <1..132
                     /locus_tag="ACHX5T_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR8469249.1"
                     /translation="LKVDPLKCELRGGQMRFTGLKRGYRLAEQVMMHEPLARIRWCG"
     gene            319..556
                     /locus_tag="ACHX5T_004342"
                     /pseudo
     CDS             319..556
                     /locus_tag="ACHX5T_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            670..928
                     /locus_tag="ACHX5T_004343"
                     /pseudo
     CDS             670..928
                     /locus_tag="ACHX5T_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            977..>1527
                     /locus_tag="ACHX5T_004344"
     CDS             977..>1527
                     /locus_tag="ACHX5T_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469250.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCS"
BASE COUNT          504 a          251 c          363 g          409 t
ORIGIN      
        1 ctgaaggttg acccgctgaa gtgcgagctg cgcggaggtc agatgcggtt tacggggctg
       61 aagaggggtt accgtctggc agagcaggtt atgatgcatg agccgctggc tcgaatacgg
      121 tggtgcggct gagagtcgca gaggggaagt tgcgtccatg ttgaggggaa cggagcaaaa
      181 aaccatcaga gatacattga atcaatcaat gtaagcaata ttaagtgtct ataccgatta
      241 cttgatgatc aggttgtgtt gaataaacgc gcattcttcg gtgctttttt aatcttttgg
      301 ggatttaaat tcctatcgat gaactgtcga tatgaaaaag cgagcatcat actgacgtca
      361 aacaaagggg tcgcagactg gggagaaatg ttcggagatc acgtgctggc aacagcgatc
      421 ctagatcggc tgctacacgc tgaatatcaa aggagagagt taccggttaa aagagaaacg
      481 taaagctgga gtgctgacca aaaacacaac gccaatcagt gatgatgaaa tggtggaaaa
      541 cggacagcat cagtaacgaa agtatcttag caggcatgaa aatcgtccgc atcccctaaa
      601 cggtcaaaat gcttctgccg tgaaggaagg agaatgccat ctcatcactt atatctggcg
      661 aatgaaacac agatggtcgg ccttgccccc aaacacacgg cggtgcaaaa tggaatggca
      721 gaaaacttcg tcaaaacgat gaaatatgat taaatcagtg ttatgttcga acctgacgtg
      781 ttaacagcag taaagtagtc tcgcgaaggt gttcaaacat tataatgaat ggcatcctca
      841 tagtgcgcag ggttatcgct tgctgcggaa atatctgaag cgacgtacca gtaatgggga
      901 aaatgatagc tggtttctga aaatatagag gaaaatgtga tatatatatc ctgttaatat
      961 ttaaggagaa tataaaatga aagtctcgtt taagtcttta ggctatattt ttcatgatat
     1021 atataataaa aaacacacta tcgatgagtt caacgatgtg gtaagaaaag cagttttatc
     1081 aggtaaaatt aacgagctga atgcatgtca taaagtggca atttttcttg ccgaaaaaga
     1141 caatgagata acaaagaaag acaaagccaa gataatagat acattaactg aaaactattc
     1201 cattgaattt caacaattaa tgaatattag tgagcgcacg ttaaattcat ctttatatat
     1261 tactcctggt gaaagtggtt ttgtcagttt tgttaatcgt gaaggaaaaa tatgtcatac
     1321 tgcatatgta aaatcatcag ataatagcat gacatattac catgcaaatg gtagcagcat
     1381 tgacaaatat attacggata tgtgcggcct tatttgtatg aggcatattg agtctactgg
     1441 cattattttt tatatgcttg atgaaaaagt tttatctgcc attgctgaat ttatgaatga
     1501 aaaaggatgg cgggctgcgt tttgttc
//
LOCUS       DAZSIO010000375         1521 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZSIO010000375 DAZSIO010000000
VERSION     DAZSIO010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.382"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..308
                     /locus_tag="ACHX5T_004345"
                     /pseudo
     CDS             1..308
                     /locus_tag="ACHX5T_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            341..742
                     /locus_tag="ACHX5T_004346"
                     /pseudo
     CDS             341..742
                     /locus_tag="ACHX5T_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1326..>1521
                     /locus_tag="ACHX5T_004347"
     CDS             1326..>1521
                     /locus_tag="ACHX5T_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469251.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILF"
BASE COUNT          432 a          301 c          364 g          424 t
ORIGIN      
        1 tgctgggcgc tgaaagaact ggcaaaggat atctggagca gaccatggag cgaggaaaga
       61 cgcaacgact ggcagagatg gctgcggcca acagtgacgt ccccatgatg aagaatgtgg
      121 ctaaaacgat agggaaaagg ttgtacggta tcctgaacgc catgcgccat ggcgtatcaa
      181 acggaaatgc ggaagcactg aacagcaaga tcaggctgct gagaataaaa gccaagggct
      241 accgcaaccg ggagcgcttt aagctgggag tgatgttcca ctatgggaaa ctgaatatag
      301 cgttctaagc cttcccacca tgatcgggga agccccagat atgaacaggc acattcagag
      361 ttactttaaa gagcttactg aacgtctgag gtgttagagc catgactgcc gcagcattgc
      421 tggcggaaat gcctgaatcg agcagcctct cgagacgtga aatcagcgca cttgtcggtg
      481 ttgctcaggt aaaccgggac tcgggaactc tgcgcggtcg acgaaccatt ttcggtgatt
      541 gtgctgggga agaacagctc tgtacatggc ggcgcttgag gccccccccc ggtttaatct
      601 ggtgataaag gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact
      661 ggttgcctgt atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga
      721 atggaatgaa tcgtatttat gaaaactgga ttgtgggcca taaatattcc aaaaagatag
      781 cccgctccgt ttattaccgg aacgggcgag agcatgacat tgtctgttat tataatgaga
      841 ctgctgattc tacttatttt taatttcaag aaaagcgttt tctacaaaga attatgagat
      901 ttaatgtttt gtttagacaa tattatcatt ctgttcaata atgaaagtct tcatcaacaa
      961 caagtaaaaa caacgtccct ctcatcgaac cggacttgca gatttcccgc atccggctct
     1021 cttgcaacac atcagacctt cacgttcggg cggttatggg gaaggtccag caagctgtat
     1081 gagccctaac tgtttgaata ccagtagatt accagaaact tctatcgatt gaagacatta
     1141 ccaactagcg ttactaaatg ttgaccaagt tttgttcact tgtctggttt tgatagtagc
     1201 attctgcaga gtattttgat atgataaata tgcattaaca atgatattat tgttaatttc
     1261 ctagcatgaa aattaaaaga tcaactttta tctcgaatat tttttacatt atcagttagt
     1321 ttcttatgaa tgacaattca ttgcttagaa attcttcatt attcatcgcc tatatgggat
     1381 gtgtagggtg ggtcagtgct tattcttatg gctggggaac ctctttttac tatggatttc
     1441 catggtgggt tgttggtgca ggtttggatg atgttgccag aagtttactc tatgctatca
     1501 tagtaatggg tatacttttt a
//
LOCUS       DAZSIO010000376         1498 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZSIO010000376 DAZSIO010000000
VERSION     DAZSIO010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.383"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            495..664
                     /locus_tag="ACHX5T_004348"
                     /pseudo
     CDS             495..664
                     /locus_tag="ACHX5T_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            810..>1498
                     /locus_tag="ACHX5T_004349"
     CDS             810..>1498
                     /locus_tag="ACHX5T_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR8469252.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWV"
BASE COUNT          366 a          340 c          462 g          330 t
ORIGIN      
        1 gtattaagac tatcacttaa ataagtgata ctggttgtct ggagattcag ggggccagtc
       61 tagatgccaa cacaaaggca actaataaag atacagataa agatctaaaa acagatctaa
      121 cccatcccaa acccttccct tccggaaggg agtttcggga ttttgtggct ggagtgcttg
      181 aggggagatt atctggcggt actgcagcgg aattttgtaa ttctgcggtg gttgcgttgc
      241 aggctgctgg cctggatgtc tgtcgtgagt atccggtgcc agagcgtggt gacggttgcg
      301 gagggcggat tgatatcgtc gtgactgaca ggaacggtgt ccggtgtggg atcgagcttg
      361 accgaaattc tccgcgacag aaatcactgc tcaaaatcgg tgctgttgaa accgggatat
      421 gtgtcttgcg gcgcagtgat atcgcaaggc acaccgagca gggaattctg gttatcggtg
      481 gggctgttcg ccagaaaaaa tttgacccgt tgtcagttga tctgcccgac tggttgtcag
      541 aaacactctg gcatgagtgg gtccagttca ggcaggcatt gcgaaaaccg attcgaacgg
      601 agctgggcgc taacggggcg atacgggaac tggaaaaatt ccgtcagcag ggttttacac
      661 ctgagcaggt gattcgacac agcatcgcca atgaatacca gggtctgttc gcgccgaaag
      721 gtgttcggcc tgagacgttg ctccgacagg ttaacaccgt ctcgttgccg gacagtgcga
      781 tcccgccagg cttcaggggg taacagacca tgaaaaatat tgcgacagga ggcgttctgg
      841 agcgtatccg cagactgacc ccaccacatg taaccgcccc attcagaacg gttgcggagt
      901 ggcgcgagtg gcaacttgct gaaggccaga aacgttgcga ggagatcaac cgcctgaatc
      961 gtcagttgcg ggtggaaaaa atcctgaacc gctccggcat ccagccgttg caccggaagt
     1021 gttcgtttgc gaattaccgg gcgcagaacg acggtcagcg acatgcactg agtcaggcga
     1081 aatccatagc tgacgaactg atgacaggct gtacgaattt tgtgttcagc ggtaagcccg
     1141 gcaccggaaa aaatcatctt gcagccgcga ttggcaaccg gctgatggcg aaggggcgta
     1201 gcgtgattat cgtcaccgtg tccgatgtca tgagcgtgtt gcatgagagc tacgacaacg
     1261 gcaaatccgg tgaaaaattt ttacaggagc tttgtggtgt tgacctgctg gtcctggatg
     1321 aaattggcat gcagcgggat acgaaaaacg agcaggtggt actgaaccag attgttgatc
     1381 gccggacggc atcgttacgc ggtgtgggga tgctgacaaa tattaaccat gcagcgatga
     1441 atacacttct cggcgagcgg gtgatggatc gcatggtcat gaacggcggg cgctgggt
//
LOCUS       DAZSIO010000377         1495 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZSIO010000377 DAZSIO010000000
VERSION     DAZSIO010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.384"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(617..989)
                     /locus_tag="ACHX5T_004350"
                     /pseudo
     CDS             complement(617..989)
                     /locus_tag="ACHX5T_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1233..1352
                     /locus_tag="ACHX5T_004351"
                     /pseudo
     CDS             1233..1352
                     /locus_tag="ACHX5T_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          383 a          338 c          338 g          436 t
ORIGIN      
        1 aagtattgaa tatattggtt ttctcacttc cgcaggtata aagtatattt tttgaattgg
       61 ttcaccgagt tgattacgtt ttcatggcta aaaattgccg cccattgtga gtcatattcg
      121 ccctaacttt ccagaaccat acgaactgcc cgttgacgga cttcggggga aaaacgtgta
      181 tttttagtca ttctgtttac ctctttctca gggcgtttag tctccaggat ttccggggcg
      241 gttcaaaaaa ctcttcgttg attactgtgg acttactgtt ggcattaccg accctgagac
      301 cggagaaata agaaccgctt agatcatcgt ggctgttctc ggggtatcaa gttacacatg
      361 ggcagaggct acccaaggtc tactattatc gcctgaaagc gctgatggaa cagtgctatc
      421 agggacatgc tgatgaaaat acctaaataa tcagccagca ctctatcttt ccaaatccac
      481 agcatagcaa agagagcaaa agagcctgta aattcagaaa ttaaggaaac gcctcttggt
      541 gtacataatc tatcttatgt taaatgaaat ctaatttctt gattatatgc tggatcgttc
      601 gtgggtggct ttgcccctat atttccagac acctgttatc attggcccgc tgccgcagat
      661 attcccgtgg cgagcgataa tccagcgcac tatgcggatg ccattcgtta taatgctcga
      721 atgcctctgc aaggttcttt gctgccgtta acccgtcagg tttgggcatg atactgatgt
      781 agtcacactt tattgttttc acgaagctct ttgctattct gttactctcc ggactccgca
      841 ccgccgtgtt cttcggttca acaaagtccc aacatccggg tgaactggcg tgtttcatta
      901 gctcggtagc atgaaccatt atccgtcagt cacttcactg gagacgccgg aagctcgttg
      961 ccgaagcggt gttccaccgc tcccagcatg tcgccaacgg gagagcatct acggtctgat
     1021 tgaactgttt attcaaattg tccaccgtct gtcagtacgc tctgaacgtc ggctggttaa
     1081 aacactgctc gctgattttc agagggttca cggtaaaaca gcacttttat tccggattgc
     1141 ggaagcggct ctgaataatc cggatgggct ggttaaagag gttgtgtacc atctgcatat
     1201 tgttgaacca gatcgcagcg gtaagagaag ttcatcatac ttggcgcagt tacgcgatgt
     1261 ttctgctaga ggtgatgccg taaagaacgg cagaacatta ccggaacaag atagcggtct
     1321 acctgcactg gtatcagacc cggggcttcc ccggatgatc tccacggtac tttaggtact
     1381 gtgtctatat tatgtaggta ggatcaatta actttgtagt tgaaattctt tcattgaaat
     1441 aatgaataaa aaagacaaac cgcattaaac aatgccagaa tataattgca ttttt
//
LOCUS       DAZSIO010000378         1492 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZSIO010000378 DAZSIO010000000
VERSION     DAZSIO010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.385"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(1..702)
                     /gene="lpfB-O113"
                     /locus_tag="ACHX5T_004352"
                     /pseudo
     CDS             complement(1..702)
                     /gene="lpfB-O113"
                     /locus_tag="ACHX5T_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
     gene            complement(750..1322)
                     /gene="lpfA-O113"
                     /locus_tag="ACHX5T_004353"
     CDS             complement(750..1322)
                     /gene="lpfA-O113"
                     /locus_tag="ACHX5T_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR8469253.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          431 a          327 c          300 g          434 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcctt taccatgaat catcacttat attaataagt
     1381 gtgtttttga ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta
     1441 attacgcaaa aataaaatat agatttattg tttaaatatt atttaaacag ta
//
LOCUS       DAZSIO010000379         1480 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZSIO010000379 DAZSIO010000000
VERSION     DAZSIO010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.386"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            198..326
                     /locus_tag="ACHX5T_004354"
                     /pseudo
     CDS             198..326
                     /locus_tag="ACHX5T_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039289737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            516..920
                     /locus_tag="ACHX5T_004355"
     CDS             516..920
                     /locus_tag="ACHX5T_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469254.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            983..1480
                     /locus_tag="ACHX5T_004356"
                     /pseudo
     CDS             983..1480
                     /locus_tag="ACHX5T_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          410 a          361 c          396 g          313 t
ORIGIN      
        1 aatgcgttcg aagtatatgt attgagctgt catactgaga aacaaacctt agggcagcac
       61 aattaaagcc agaaacaact atgcaagaaa gttactaaat aagtgctcgg aacagtaaaa
      121 cactcagggg ggactcagtc cggattatgt aatcagataa attgccaaac atcacaaatc
      181 accagccgga ctgagtgatg ccgagcattg caccaatacc ccgtgatgaa cgatgcctga
      241 tgcagaaagc tccctataaa acaaagaaca aaaactacga ccggcgattc accgtcatac
      301 tgatgctgca tcgtggtgac actgtctgtc actttcatgt tgactgaaca actctgctgc
      361 gaagaaggtg taccgccggc gggatttgat tgtccggcgt ctagcttcca cttccctgac
      421 ggaactgaaa gttgttatgg ccggtcagct tgccctgtcc gcaggacatg aggtgctgtg
      481 acggacagca ccagtaacgg aaagtgacaa tgaaaatgaa tactgcgctg atagttgcac
      541 taatgtgtat gtggtatgcg gtacccgctg ctgcaaaaga gactttactg gctatgccac
      601 gtaattcaac agaacattgc tatgcggaga ttaatgtgca cggaccatat ggggtgtatt
      661 tccgagtggt cccgcatccc cccgggggaa agagctgggt cgaatgtaac agtgactact
      721 attactctga taaaccaccg ggagtacaga tactcggaac ccgtgcaggg tgtcgggtat
      781 acggaatctg cgggaccacc tcgacactgc atgtggctgg aagaggggtt gtgtgtatta
      841 aaaatatatg cagtccacgt gggatgataa ttcacaggat acggaaacgt cctgtagtgg
      901 ctgtaagcga tgagatgtga tcttacccag caatagtaga cacgcggcta agtgagtaaa
      961 ctctcagtca gaggtgactc acatgacaaa aacagtatca accagcaaaa aaacacgtaa
     1021 acagcattcg cctgaatttt gcagtgaagc cctgaagctt gctgaacgca tcggtgttgc
     1081 tgccgcagcc cgtgaactca gcctgtatga atctcagctc tatgcgtggc gcagcaaact
     1141 gcagcaacag atgacctcgt ctgagcgtga aagtgaactg cccgcctgaa gcggcaactg
     1201 gcggagcagg cagaggaact ggctatcctc caaaaggccg cgacatactt tgcgcagcgg
     1261 ctggtatacg tggtgtcagc gtcggacagg aataagccct cgtcagcagt tccgtcagca
     1321 ctgcgacagc gttgtcctcg cggcggcctt tacccggtca aaacagcgtt acggtgcacc
     1381 acgcctgacg gatgaactgc gtgctcaggg ttaccacttt aacgtaaaaa ccgtggcggc
     1441 aagcctgcgc cgtcaggggc tgagggcaaa ggcctcccgg
//
LOCUS       DAZSIO010000380         1478 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZSIO010000380 DAZSIO010000000
VERSION     DAZSIO010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.387"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            98..1018
                     /locus_tag="ACHX5T_004357"
     CDS             98..1018
                     /locus_tag="ACHX5T_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR8469255.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1018..1377
                     /locus_tag="ACHX5T_004358"
                     /pseudo
     CDS             1018..1377
                     /locus_tag="ACHX5T_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(1394..>1478)
                     /locus_tag="ACHX5T_004359"
     CDS             complement(1394..>1478)
                     /locus_tag="ACHX5T_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469256.1"
                     /translation="LIALARRRCDVLFAMMRDGTFYTPQGS"
BASE COUNT          404 a          338 c          372 g          364 t
ORIGIN      
        1 gtctaatcat gatttagggt ctaatcaact taatgatcaa tatctatagt gatatcacca
       61 accgtcaaaa cgaaccgaca actccatgag gaactgaatg atgaataata aagtcagctt
      121 cactaacagc aataatccaa ccatctcttt gtctgcagtg atctatttcc ctccaaaatt
      181 tgatgaaacc cgtcagtatc cggcaattgt ggtatctcac ccaggtgggg gcgttaaaga
      241 gcaaacggcc ggaacctatg ccgaaaagct ggcggaaaag ggatttgtca ctattgctta
      301 tgacgcatct tatcaaggtg aaagtggcgg cgagccgcgt cagttagaaa acccatacat
      361 tcgtactgaa gacgtcagtg ccgtgattga ttacctgaca acgctttctt atgtcgacaa
      421 tacccgaatt ggtgcgatgg gaatttgtgc cggtgcagga tatacagcca atgctgcaat
      481 tcaggatcgc cgcatcaagg ccatcggtac cgttagtgcg gtcaatattg gttcgatgtt
      541 ccgcaatggt tgggaaaaca atgtgaaatc tattgatgct ctgccacaca ttgaagctgg
      601 ctcaaacgcc cgaaccagcg atattagcag cgaagaatac gccattatgc cactggctcc
      661 aatgaaggag gccgatgcgc cgaacgaaga actgcgtcag gcctgggagt attaccacac
      721 acctcgtgcg cagtatccaa cagcaccggg ttacgctact ttgcgcagcc ttaaccagat
      781 tattacctat gatgcttacc atatggcgga agtgtacctg actcagccga tgcagattgt
      841 ggcgggcagc caggcaggga gcaaatggat gagtgacgac ctgtacgatc gggcatcaag
      901 ccaggataaa cgctatcaca ttgttgaagg cgcaaaccat atggatcttt atgacggcaa
      961 agcctatgtg gctgaagcaa tttcagtatt agctccgttc tttgaggaaa ctctgtaatg
     1021 aaacatattc aaatccgcaa tagtgatatg gcctggcata ttgctgccaa tattcagttt
     1081 ccaccaaatt ttgatgagtc aaaacagtat ccggcgatta tcagcgtcca tccttttggc
     1141 agttgtaagg agcagacgtc tggcaatatc tatggcaagg cattggctga aaaaggctat
     1201 gtcgttctcg cttatgacgc cagtttccag ggagaatctg gtgggtagcc tcgttggata
     1261 gaagatccga cacagcgcgt agaggatatt agccgtgtta tcgattatgc agtaactctg
     1321 ccatatgttg atgctgaacg cattggcgtg ctgggtgtgt gtggggggtg cccctattaa
     1381 gttgtcaagc atgttatgac ccctgcgggg tataaaaagt cccgtcgcgc atcatggcga
     1441 acagaacgtc gcagcgtcgt ctcgccaggg cgataagc
//
LOCUS       DAZSIO010000381         1443 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZSIO010000381 DAZSIO010000000
VERSION     DAZSIO010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.388"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            82..1275
                     /locus_tag="ACHX5T_004360"
     CDS             82..1275
                     /locus_tag="ACHX5T_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HFR8469257.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            complement(1295..1443)
                     /locus_tag="ACHX5T_004361"
                     /pseudo
     CDS             complement(1295..1443)
                     /locus_tag="ACHX5T_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT          422 a          313 c          339 g          369 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt ggtcttcggg ccagcaaaag tcgttcaaca
     1021 ggaagaattc tggttctgag tctgctggca acgttgagca ctatcgtaat gtggttactc
     1081 ggttatcacg ctgaaaataa agggttacac ctgaaatatc aggctaacag cattaaatcc
     1141 cggcgggtta tctcatatct gacgttagcg aagaatgttc ttcgacactc gccacttatt
     1201 ttaagacgaa cagtactgag cacagttcta aaccatctgt ccagaaccta ccgaaatatg
     1261 gtgctggttt attagcagta attttgtggg gatccctcag cctgaagggg cgacacagtc
     1321 ggggagagaa gcccatctcc ttgccgaact gccacagcga aggatggaac cggtgctgac
     1381 cggtctgata tgcgtcacgt tgcagaacca cagttttcat attgtcatac aacacttcgc
     1441 gcg
//
LOCUS       DAZSIO010000382         1424 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZSIO010000382 DAZSIO010000000
VERSION     DAZSIO010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.389"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..950
                     /locus_tag="ACHX5T_004362"
                     /pseudo
     CDS             1..950
                     /locus_tag="ACHX5T_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          336 a          373 c          404 g          311 t
ORIGIN      
        1 ccctgtatcg ccagcagcac attttctccc gaatgggtgt ggagctcccc gtcagtacaa
       61 tggcggatat ggtcggtgtt gccggagctg cactggcccc gctggcgaaa ctgctacgcc
      121 atgagcttct gacacgggat gttattcatg cagatgaaac gtccctgagg ctccttgata
      181 cccggaaagg cggtaaatcc tgctccggct ggctgtgtgc ttacgtcagc ggtgaaagaa
      241 gcggtccgcc ggtcgtctgt tttgactcgc agaccggacg ggccctcaga taccctgaga
      301 cctggctgca gtgctggtgt ggcagtacgc tggtgagtga cggatacagc gtgtacaaat
      361 ctctggcgga taatcacccc ggcatcacat cagcctgttg ctggagtcac gcagggcgcg
      421 gcttcgcaaa tctgtataag gccagcaggg agccccgtgc aggcgtggag ctgagaaaaa
      481 tcgcgggtct ttaccgcatt gagaaactca tccgtgagcg ccctgtggaa aaaatacggc
      541 agtggcgata acggtactcc cggccgatag tgaatgacct gtttgcctgg cttgaagagc
      601 aggagccgtg ctgtccgccc gacgggccat taaataaggc gattaactat attctgaacc
      661 ggcgcgatga actgagctgc ttcctgggtg acggagctgt gcccctggac aacaatatct
      721 gcgagcgtgc cattcgcccg gttgttatgg gacggaaagc gtggctgttc gccggttcac
      781 tgatggccgg aaaccgggcg gcacagataa tgagccttct ggaaacagca aaacgtaatg
      841 gtctggagcc acatgcctgg ctgacggacg tcctgacgcg tctgccggaa tggccggagg
      901 agcgactggc agagttgctg cctcttgagg gatttacctt caccgggtga gtgatacctg
      961 ccgtcaggtg ttcgtgaacc gggctataac ctgcagtcgg ttgttgaacg cctgacaaca
     1021 ggaaatcagc caggccagca accacgcgga ctgacagaga atcagaacct gaatcagcct
     1081 gaaatcatac ccggggcagt acttcatcac cagtcaccaa tacccgtgtc agtacttcgc
     1141 ggcctgacgc gatcacccga ctcagtactt ctgagcccgc ctccggtacc attctctgtg
     1201 ggcccgtaca caacaacaga aaagggtgcc cggcccgggg cgcaaccgta ttacacttcc
     1261 ggaaaatcgt tatacgcaga aaaatcggat cacgctgtgc ctgctttcat gtaatttact
     1321 tacggtgtta taagtatgct aattatagta taaaaaaatc gcagcagaaa aaattgtatt
     1381 aaaaacttat agcgaattaa tggtgtactt tttatggata ttga
//
LOCUS       DAZSIO010000383         1418 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZSIO010000383 DAZSIO010000000
VERSION     DAZSIO010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.390"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            39..443
                     /gene="stpA"
                     /locus_tag="ACHX5T_004363"
     CDS             39..443
                     /gene="stpA"
                     /locus_tag="ACHX5T_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR8469258.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(490..1014)
                     /gene="ygaP"
                     /locus_tag="ACHX5T_004364"
     CDS             complement(490..1014)
                     /gene="ygaP"
                     /locus_tag="ACHX5T_004364"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR8469259.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1024..1323)
                     /locus_tag="ACHX5T_004365"
     CDS             complement(1024..1323)
                     /locus_tag="ACHX5T_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR8469260.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          383 a          332 c          335 g          368 t
ORIGIN      
        1 atactttttt gttttggcgt taaaaggttt tctttattat gtccgtaatg ttacaaagtt
       61 taaataacat tcgcaccctc cgtgcgatgg ctcgcgaatt ctccattgac gttcttgaag
      121 aaatgctcga aaaattcagg gttgtcacta aagaaagacg tgaagaagaa gaacagcagc
      181 agcgtgaact ggctgagcgt caggaaaaaa ttagcacctg gctggagctg atgaaagctg
      241 acggaattaa cccggaagag ttattgggta atagctctgc tgctgcacca cgtgctggta
      301 aaaaacgcca gccgcgtccg gcgaaatata aattcactga tgttaacggt gaaactaaaa
      361 cctggaccgg tcagggccgt acaccgaagc caattgctca ggcgctggca gaaggtaaat
      421 ctctcgacga tttcctgatc taattactga ggccggatgt cgctgctagg gcgcgtccgg
      481 cgtcgcttct caagctcgtt ggttccacgg catcttatct aacaaccttg ccattccaca
      541 aaaaccgctg attcctgcaa acagtaaccc ggcaccaaca aagccactta ataagaagaa
      601 accgctattt acggtataac ccagtacaac gccgtttaat attaaaccac ccgcagcgat
      661 ctgcacctgg cgcattaacg gcaagggttg agatttattt accgctactg gcaatccagc
      721 tcttttccag ccatcaatcc catcttcgag taaaaatatt tctgcggggg cggcaatcgc
      781 tgctaattta tcggcgttat tactggtgcg tttacctgcc tggcagtgga atataatttg
      841 ctcgcgacgt aatttaggtg gaagacctga ctgttccagc acggataatg gagccagatc
      901 tgcttcagga atatgttcac gaagatattc atcagcgtca cgaatatcga ttaactttgc
      961 gccgcgtgcg attaattctt gtgcatcatg cggcgaaatg gttgtcaaag ccatagtgac
     1021 tccttacgga caatagacat ttttcagggt ggcgataatg gcatttaccg cctcattttt
     1081 aatggaatat agaatgcgtt gggcatcccg ttggctgttg ataagccctt cgtcccgcat
     1141 acgagcgaga tgctgtgatg tcgcagaggc gctcagtccg gtaatgcgcg tcagctctcc
     1201 cgcgctggtg ccgggggaac cgctaagcat gcacagaatc agcaaccgtt tagggtggct
     1261 cattgctttc aataaggccg ctgcctgttc ggtactggcc tgtaattgcg cgagttcagt
     1321 catatttatt taagtatttt ctaaattaag taaactctaa actaaaaatg caacatatac
     1381 cagcctcagc agcgtaaatg agagtaaaag cgtaagct
//
LOCUS       DAZSIO010000384         1414 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZSIO010000384 DAZSIO010000000
VERSION     DAZSIO010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.391"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..294
                     /locus_tag="ACHX5T_004366"
     CDS             <1..294
                     /locus_tag="ACHX5T_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR8469261.1"
                     /translation="SAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLFE
                     SLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            300..1397
                     /locus_tag="ACHX5T_004367"
     CDS             300..1397
                     /locus_tag="ACHX5T_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR8469262.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSIRIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIESLNLGGEHPGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIALDEIIELLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNAL
                     WIELEGVEYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          438 a          320 c          347 g          309 t
ORIGIN      
        1 agcgcttatc cgatttttga tcgtattgaa gaaatggcat gggctcgtca ctaccagcag
       61 atcgttcgtg aagaaaaaga aacggaactg gcggacgacc tggaaaaagg tctgcctcag
      121 cacctgtttg aatcgctctg catcgaccat ttgcaacgcc acggtgccaa taaacaggca
      181 atcagccacg catttgatga tgatgttgag tttcaggagc gcatggcaga acacatccgg
      241 tacatggctg aaaccattgc ccaccaccag gttgatattg atttggagga ctaaaatcta
      301 tggcgctttt tcaacgagca ataaacacac aggcctatct aaaagcagga attatggggt
      361 ttgctggcga cggcaaaacc tacacagcaa gcgaactggc tattggtctg gtaatgctta
      421 tgcggcaacg gggaattgaa gctggtaacc gcccggtaat gtttctggac acagaaacag
      481 gctctgactg ggttaaaccc cgctttgatg cggaaaatat cgaattattt acggcaaaaa
      541 cccgctcgtt tgttgatctg cttgaagcca ttaatgaagc ggaaagcagc ggttccgtaa
      601 tgatcatcga ctccatcagt cacttctgga ccggattatg cgacgagtat gccagacgcc
      661 gaaaccgtaa acgaggactg gaattttcag actgggcgtg gctcaaacag gagtggcgac
      721 gctttaccga tcgatttgtt aacagtcagg cgcacattat tatgtgcggg cgtgcaggat
      781 atgagtatga ctttttcgaa ggtgatgatg gcaaacgcca gttggcaaaa accggtataa
      841 aaatgaaagc agaaacagaa accggttatg aaccctccat ccggatccag atggagaagc
      901 aaatgaacat agaaaccggt caggtgtggc gcaccgccag aatactaaaa gatcgctcca
      961 cccgtattga tggacaggta ttttcaaacc caacatttaa aaactttctt cctcatatcg
     1021 agtctcttaa cctgggaggt gaacaccccg gcatcgatac ctcacgggat aacagtgaat
     1081 tatttgccaa cgacggtaca ccgacatggc taaaagaaaa gcgtgcaaaa gaaatcgcac
     1141 ttgatgaaat tatcgagttg ctcaacaagc accatggggg caccagtaac gacgccaaac
     1201 gcgcaaaagc tgatttgctg gaaaaaacat tcacctcccg ctcatgggaa agaatcaaag
     1261 gtatggactg gccgacaatc aaagctggtc gtaatgcact atggattgaa ctggaaggcg
     1321 ttgaatatgc attcccggat ccacaaacac aaaacgaaac gcaacaagca ggatacgatg
     1381 agaatattcc ggtgtaaaac caagtgcatt ttgg
//
LOCUS       DAZSIO010000385         1404 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZSIO010000385 DAZSIO010000000
VERSION     DAZSIO010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.392"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..167
                     /locus_tag="ACHX5T_004368"
     CDS             <1..167
                     /locus_tag="ACHX5T_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFR8469263.1"
                     /translation="AIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRM
                     KERRKEWGTL"
     gene            152..790
                     /locus_tag="ACHX5T_004369"
     CDS             152..790
                     /locus_tag="ACHX5T_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFR8469264.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1018..>1404
                     /locus_tag="ACHX5T_004370"
     CDS             1018..>1404
                     /locus_tag="ACHX5T_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469265.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          407 a          257 c          347 g          393 t
ORIGIN      
        1 ccgctattgc cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga
       61 atgatttctg gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt
      121 acctgcagcg tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact
      181 tggcaacatg attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc
      241 tctgaatcat ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga
      301 ctgtgtgttg tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat
      361 ggcactaggt gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc
      421 ggtggtggtg tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt
      481 actggggcgg gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat
      541 taatccggag agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc
      601 cagggggaaa caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg
      661 ctggacgaat gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct
      721 gaaacagatt agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac
      781 ggttcgttga tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa
      841 aaatgatacc gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta
      901 ggctttgata acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg
      961 cagatttaca aaatctgatt gattctaaag atgtataagt aaaaatcaag ctttgatatg
     1021 aaaaaacaga tatttattaa taataaacct cctgtagtcc cttactcagg gacgcacgcg
     1081 aaaatattta aatacattga aatccctctg ccgtttttct actttatcta tacatcaggc
     1141 gaaccattcc acatatctgt ccaaaatact gttatttatg tatcaaaata caatggtata
     1201 tttattaata aattagttcc tttttctctt ttatttgaca gggatatatc tgttcttcaa
     1261 agaagagata tttgtgttgt gcgatttacg tcagaggaaa taagtgaaca taacgtttta
     1321 tttgatcatg acattgaacg attaaaaaaa atttccaaag ctcagcttat atctcccgat
     1381 tatgttttga ttgatttttc tagt
//
LOCUS       DAZSIO010000386         1402 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZSIO010000386 DAZSIO010000000
VERSION     DAZSIO010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.393"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..1283)
                     /gene="mxiA"
                     /locus_tag="ACHX5T_004371"
                     /gene_synonym="virH"
     CDS             complement(<1..1283)
                     /gene="mxiA"
                     /locus_tag="ACHX5T_004371"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HFR8469266.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIY"
     gene            complement(1296..>1402)
                     /locus_tag="ACHX5T_004372"
     CDS             complement(1296..>1402)
                     /locus_tag="ACHX5T_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YopN family type III secretion system gatekeeper
                     subunit"
                     /protein_id="HFR8469267.1"
                     /translation="ESPHDIFKSESEREIAINILRELVTSAYKKELSR"
BASE COUNT          509 a          295 c          195 g          403 t
ORIGIN      
        1 aaatatatat taaaactatc ggcacgcacc tcatttatta acaaaacaat atcatcgacc
       61 ttaagctcat tgcttgttct atataaaata gttggaagcc tgacaccata atcaataaag
      121 aactgacttc ttatcctttc aataagacct tccatatcat ttgcatttat cttattttcg
      181 gcaaataata aaattaaagg aacggtttct gaactaatag catccagatt ttctatcatt
      241 gccaaagatg agtcatggct attatcaata tcaaatgtac cagtatagcc actagaatca
      301 gactcagaca gacttttttc tttttctacg acctttttat aatagaataa agccgtcaaa
      361 gtaactgcta tcaggaaaaa aacaaaaaac ggaaaacctg gcaacatccc tatcgccaag
      421 gcaagagctg atgtaacaat gagaacaaaa ggattcccaa atatctggga cattatgtta
      481 cgtcccatat tatcgctatc accattaact cttgtaacga taaatcctgc acttatagaa
      541 attaacagag caggaatctg agagaccaat ccgtcaccaa tagttagtat ggtataagta
      601 gataatgcac cagataggga cattccatgt tgactcatac caacagaaat acctcctatt
      661 aaattgacaa aaataataat gatgccagca attgcatcac ctttaatgaa cttcattgct
      721 ccatcaaatg aaccatacaa ttgactctca cgttccagaa tactgcgtct ttctttagct
      781 cctgcggcat caataattcc agcctttaaa tccgcatcaa tgctcatttg tttccccggc
      841 ataccgtcaa gagaaaagcg agcagctacc tccgcaaccc tttcagatcc ttttgttata
      901 acaatgaact gcacaacagt tactattgaa aaaataacaa aacctacagc cagactatca
      961 ccaatgacaa actggccaaa tgtagtaata attttacccg catccgcatc aactaaaatt
     1021 agtcgactag tacttattga taaagcgaga cgaaataatg tcgttatcaa caaaaccgaa
     1081 gggaatgtag aaaaactcaa tattctttca atataaaatg atcccataaa aacgagaata
     1141 gcaagtacaa tattaagccc tattaaaaaa tcaacaaggt atgtaggtaa tggtatgatc
     1201 aacatagcaa tgatcataac cataagcact aatataatta attcaggctt agtacttact
     1261 tgcttaagaa aagactggat cacttttatc tcctgttatc tagaaagctc tttcttgtat
     1321 gcacttgtga caagctctcg tagaatattg atcgcaattt ctctttcact ttcactttta
     1381 aatatatcat gaggactttc at
//
LOCUS       DAZSIO010000387         1397 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZSIO010000387 DAZSIO010000000
VERSION     DAZSIO010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.394"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            321..>1397
                     /locus_tag="ACHX5T_004373"
     CDS             321..>1397
                     /locus_tag="ACHX5T_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8469268.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRAFSAFLDRLSDT"
BASE COUNT          449 a          315 c          222 g          411 t
ORIGIN      
        1 ttactatgta tataagctta ttttatacat gattattagc aacaaatatt accaagtcaa
       61 gtagtcaggc tggctaaaat agttggagat gttgtagtat cttactcaag actaatgtcc
      121 tctggtataa atcctctgca ttgtgaatat gtaaaacatc aaaaaaagat gaaccaaata
      181 tacgaaaaaa gcaaatatac ttaccagggt agaaagcatg atataacact cattccctta
      241 tggcaaatat tctgaaacac cctcctcatt aggagagata cattaaatac aatttattca
      301 caatctaatt ccatatccat atgcgagaaa ttaatatgct cagaaacatt tcatcctgtt
      361 tatttccaca tatcagcaca attacatccc ccaaccatta tttgtccgaa tgggatgatt
      421 gggagaaaca ggggttaccg gaagaacagc gtactgaggc ggtaagaaga cttcgtgcat
      481 gtcttacctc taaggggcat aaactggacc tgcgagcctt ggcgctttcc tcgctacctg
      541 tactccctgc ttgcattaaa aagcttgatg tgagctgtaa taaattaacc atccttactg
      601 atttacctga aaatattaaa gaacttattg caagagataa tttcttaaca catatatctg
      661 cattaccaca ttatctaata actttggatg tgtccgaaaa tcaattagag aatctgccgt
      721 tattaccaga caccatcaaa tcactaagcg cagagtataa taggttatcc acactgcctt
      781 cattaccctt gaatttaaaa aaacttgagg ttaggaacaa cgaactgcaa actcttccat
      841 ctctgccttc taatcttaag atacttaagg ttgcgcacaa ccatcttact gaactgcccc
      901 ctttacctag gagactgcaa cttctttttg catatagcaa tagattaagc aacttaccaa
      961 acatccaaga aaatattatc atgagaagat ttttttattt tgaaaacaac caaataacta
     1021 caatcccgac aaatcttttt cgtttagatc ctcatataac tattgagatt gcaaataacc
     1081 ccttatcaga tcaaactctg ctattcttaa tacagcaaac ttcggttcca aattttaacg
     1141 ggcctcagtt tcgtatttcc ctgtcagacc aaaacagact gtttttacgc cagatgttgc
     1201 cgcaaaattt acattcgcgc catatcagag tcatcactga aggggggcag aactttcaga
     1261 tcccccctct tcccgaaact gtggcagcct ggtttcctga agcagatcgt cgggaggttt
     1321 ctacacaatg gacttctttt tccaccgagg agaattcccg ggcattctcc gcgttccttg
     1381 accgcctttc cgatacc
//
LOCUS       DAZSIO010000388         1373 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZSIO010000388 DAZSIO010000000
VERSION     DAZSIO010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.395"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(424..672)
                     /locus_tag="ACHX5T_004374"
     CDS             complement(424..672)
                     /locus_tag="ACHX5T_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR8469269.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(935..1030)
                     /locus_tag="ACHX5T_004375"
                     /pseudo
     CDS             complement(935..1030)
                     /locus_tag="ACHX5T_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1027..>1373)
                     /locus_tag="ACHX5T_004376"
     CDS             complement(1027..>1373)
                     /locus_tag="ACHX5T_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR8469270.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          336 a          292 c          332 g          413 t
ORIGIN      
        1 tcctaaactg gatcggttta atgtaaaaaa acgatacttt ttgggggggg gcaatttttc
       61 cttaaggtat gaaaaaagga gctgaagcta tataatagct attaatgcca ctggtatcag
      121 tgaatcatgt atataaaaaa ctgtgttaag gaggctgtcc attagcggtt ttttattctt
      181 ctggaaccgc ggcatctttg cgtatctttt tgtcttctca ccgtctggtc ggtgttttgc
      241 tgagactgct aatttcctgt ctttgctagt gctgtactgg tactgcccaa tcatgattgg
      301 cgggggcggg cggagttgag accgcagaca cgtcgtatgc aagaacgtgc tgcggctggc
      361 tggtgaactt ccgatagtgc gagtattgaa tgatttccag ccgttactga ttttacgtgc
      421 taattagtga acaaaccact cgtcagcaga ctcccaggta tctttcagag tttcctgaac
      481 aaaagtttta gctgaatctt tatcggcggt gcgcgtaaca gaaaggccat cgttgctggt
      541 ggcttttacg atcacctcta catcgtcata acgtttactg atgcgtcggg ttaattcttc
      601 ctttaacgca tccacagcac cggttggcat tttagtcatt ttctctttgg ctatgcagat
      661 ttcaaaacgc ataaaaatcc ctctgcactg tgtttttata cagtattatt tttaactgta
      721 tggataaaca gtgtcaagag tgagggaaag tgattttttg gggcatgcat ggggcaaaaa
      781 tatttgttgt ggggcatttt ggggcatgat tgggacactt tgttacatat gaactttgcc
      841 gattttcata tgacatggca atccaacgct ttgattattc taaagaaaac acatgctctt
      901 gggcgttctt tagtgatttt taaaaatccc gtatcacgca gttaaagtgg cgggcatact
      961 cttcaaggct ggtgatcccc aaccgcaccc atttaggatg tgaccattgc ggtagaccga
     1021 tgtaaatcat aacgcgtgaa ggatctcttc cacgctacgc acacgggcga tgcgcgggta
     1081 gatatgatta atgctgttat tgtgttgctc ggcgctggcg gcactacagg cgtcttcggc
     1141 aatcaccaga ttaaagccaa gttcccaggc attgcgggcg gtggattcaa caccgatatt
     1201 ggtcgagatc ccacataaca ctattgtatc gatacctcgg cggcgcagtt gcaactccag
     1261 atccgtaccg taaaacgcac cccactgacg tttgatgatt tcgatatcac tgtcagttgc
     1321 acctaatgca gctggatgtt gccaccagtt ttcgggcaac actttagcgg ggg
//
LOCUS       DAZSIO010000389         1353 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZSIO010000389 DAZSIO010000000
VERSION     DAZSIO010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.396"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..1043
                     /gene="ospD3"
                     /locus_tag="ACHX5T_004377"
     CDS             <1..1043
                     /gene="ospD3"
                     /locus_tag="ACHX5T_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HFR8469271.1"
                     /translation="DRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQ
                     LSDLSDNTLELLLAAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLT
                     AEGMGGRISGLSQALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQI
                     PGLMFALQNGHADAIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQAD
                     AILAYGDILNEAKLNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADL
                     TPHQASKLLAAEGPNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNG
                     SDFLEIMKNIKS"
     gene            1160..1279
                     /locus_tag="ACHX5T_004378"
                     /pseudo
     CDS             1160..1279
                     /locus_tag="ACHX5T_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          441 a          268 c          284 g          360 t
ORIGIN      
        1 tggacagaca tactccaaca agcatgtcct ccataatcag atggcctgat aatttacttc
       61 accccaaagt tatttatcac gcgatgcgta tgggattgac tgagctaatc caaaaagtaa
      121 caagagtcgt acaactatct gacctttcag acaatacgtt agaattactt ttggcagcca
      181 aaaatgacga tggtttgtca ggattgcttt tagctttaca aaatgggcat tcagatacaa
      241 tcttagcata cggagaactc ttggaaactt ctggacttaa ccttgataaa acggtagaac
      301 tactaactgc ggaaggaatg ggaggacgaa tatcgggttt atcccaagca cttcaaaatg
      361 ggcatgcaga aactatcaaa acatacggaa ggcttctcaa gaagagagca ataaatatcg
      421 aatacaataa gctgaaaaat ttgctgaccg cttattatta tgatgaagta cacagacaga
      481 tacccggact aatgtttgct cttcaaaatg gacatgcaga tgctatacgc gcatacggtg
      541 agctcattct tagcccccct ctcctcaact cagaggatat tgtaaatttg ctggcctcaa
      601 ggagatatga caatgttccc ggacttctgt tagcattgaa taatggacag gctgatgcaa
      661 tcttagctta tggtgatatc ttgaatgagg caaaacttaa cttggataaa aaagcagagc
      721 tgttagaagc gaaagattct aatggtttat ctggattgtt tgtagccttg cataatggat
      781 gtgtagaaac aattattgct tatgggaaaa tacttcacac tgcagacctt actccacatc
      841 aggcatcaaa attactggca gcagaaggcc caaatggggt atctggatta attatagctt
      901 ttcaaaatag gaattttgag gcaataaaaa cttatatgga aataataaaa aatgaaaata
      961 ttacacctga agaaatagca gaacacttgg acaaaaaaaa tggaagtgat tttctagaaa
     1021 ttatgaagaa tataaaaagc tgaatattat ctcgtggtga tacactgaat ttgaggtaac
     1081 cggctcattt aaaccgtctg gtctgttacc tccggttcca caaaaataat gtccatcatt
     1141 ttgaagtgga cactatcgta tggaataccg gacctggatc actgaagctt tacgccttca
     1201 tttcgaagaa catttaccac gggttgtggc cggacgtcgc ctgggcgtac caaaatcaac
     1261 tgtctgtggt atgttcgtgt gaaccgcccc cggtatcccg ctcatgctgg cgaacccaga
     1321 cacgcagtga accgccccgg gtttcctgga gag
//
LOCUS       DAZSIO010000390         1337 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZSIO010000390 DAZSIO010000000
VERSION     DAZSIO010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.397"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(208..363)
                     /gene="hokD"
                     /locus_tag="ACHX5T_004379"
     CDS             complement(208..363)
                     /gene="hokD"
                     /locus_tag="ACHX5T_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR8469272.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(591..1256)
                     /locus_tag="ACHX5T_004380"
     CDS             complement(591..1256)
                     /locus_tag="ACHX5T_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR8469273.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          331 a          342 c          314 g          350 t
ORIGIN      
        1 tccatccctc ttctccctgc gcgctactta agtgagtcga ttctatctgc gaaggtgcgc
       61 gaatcaaatt caccggaaat aaacaacaaa aaacccgccg aaacgctgag agatcacagc
      121 gatgggcggg ttaagtgcgg gtgcgttgag gatgcctgac acatcagagg tgggcgggga
      181 tgggatcagc tccccacccg gtcactctta cttcctggat tcgtagtcta cgaagacagc
      241 gacctccgtc tggccggttc ggattcgtac ctcacagagg tctttcctcg ttaccagtgc
      301 cgtcactatg acggttaaac agatgacgat cagggcgatt aacatcgcct tttgctgctt
      361 catagcctgc ttctccttgc ctttcggcat gtaagaggct aacctacatg tgcaaagcat
      421 gaaattggcc tcagattaat gttaagcgtc ttgccggacg cgtaatgtta actggggctt
      481 ttctctatct gccttttggt gttcatgcct gaagcagata gcctcaagca cccgcagtca
      541 ttctacttac ctcgccaata tgaaatcaat cagaaaggtg ccccataaaa tcactccttc
      601 tcttctttac cgtagtggag ttgaccaatt ttgataagag ggcgtccctg agatttgcgg
      661 tgtagattgg tatcgcgcag agaatacaca cagccacaat attcctgctg atagaatttt
      721 tcgcgcttgc tgatttcaat catacgggac gagccgccct gcttgcgcca gttataatcc
      781 cagtacacca tgcccggata atgcgcgacg gctcgccgcc cacagtcgtt aacctgctgc
      841 atatttttcc agcgtgaaat gcccagtgaa ctgctgatca cactgaaacc attttcagcg
      901 gcgtacagcg ctgtccgctc aaaacgcatg tcaaaacaca tggtgcaacg gatccctcgt
      961 tcgggctccc attccattcc tttggctcgt tcaaaccagt tgtctgtgtc gtaatcagca
     1021 tcaataaacg gcacgccgtg ttgttcagca aagcgaatat tctcatcctt acgaattaaa
     1081 tactctttct gaggatgaat gttcgggttg tagaaaaaga tggtgtagtc gatttccgag
     1141 gcctgaagcg cctccatcac ctcaccggaa catggagcac agcaagagtg cagtagtagt
     1201 ttgtttgccc cgtttgggag ctccaatttg ggccgtttga aatcagcaac tgtcataaat
     1261 atgtttattg aggtcataaa aatatcaaag agtgtagcat tagaacgggg ctatcggaaa
     1321 cagatgtgtg actgctc
//
LOCUS       DAZSIO010000391         1330 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZSIO010000391 DAZSIO010000000
VERSION     DAZSIO010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.398"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(41..100)
                     /locus_tag="ACHX5T_004381"
     CDS             complement(41..100)
                     /locus_tag="ACHX5T_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR8469274.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..1265)
                     /locus_tag="ACHX5T_004382"
                     /pseudo
     CDS             complement(339..1265)
                     /locus_tag="ACHX5T_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
BASE COUNT          266 a          354 c          321 g          389 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttatgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 tgttcggtaa tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc
     1321 ttgtcatgca
//
LOCUS       DAZSIO010000392         1302 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZSIO010000392 DAZSIO010000000
VERSION     DAZSIO010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.399"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            168..>1302
                     /gene="pdeF"
                     /locus_tag="ACHX5T_004383"
     CDS             168..>1302
                     /gene="pdeF"
                     /locus_tag="ACHX5T_004383"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR8469275.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT          297 a          284 c          288 g          433 t
ORIGIN      
        1 taattcatta atcagggtta ttttgtttga tatatatcaa ttggaacttt catatgacac
       61 ttagaatcaa tgctctctat agtgatgaat aatcatcatt cgaagtcagg tgggatgcct
      121 gtctgaatac acctccttca ggatgtgggg gattctgctg agcatctatg aaactgaatg
      181 caacttatat aaaaatacgt gataaatggt gggggcttcc gctgttcctg ccttctttaa
      241 tcttgcccat tttcgcccac attaatactt tcgcgcatat ttcttccggt gaggtttttc
      301 tcttttatct gcctttggca ctgatgatca gcatgatgat gtttttcagc tgggcggcat
      361 tgccagggat caccttaggg atttttgtcc gcaaatatgc agagctgggt ttttacgaaa
      421 cgctctcatt aacggctaat tttattatca ttatcattct ctgttggggc ggttacaggg
      481 tctttactcc ccggcgtaac aacgtttcac atggtgatac ccgtttaatt tcccagcgta
      541 tattctggca gatagtgttt cctgcaacgc tgtttctgat acttttccag tttgctgcat
      601 ttgtaggatt actggcgagc agagaaaatc tggtcggtgt catgcctttt aacctcggga
      661 ctttaatcaa ttatcaggcc ttactggtgg gtaatctgat cggtgtcccg ctgtgctact
      721 tcatcattcg ggtagtgcga aatccatttt atttacgtag ctattattcg caattaaaac
      781 agcaggttga tgccaaagtc accaaaaaag agttcgcgct ctggctactg gcattaggtg
      841 ctttattgtt gctgttatgc atgccgttaa atgaaaaaag cacaattttt agcaccaatt
      901 ataccttgtc attattgctg cccctgatga tgtggggagc aatgcgctat ggttataagc
      961 tgatttcgct gctctgggcg gtcgtgttga tgatcagcat ccacagctat caaaattaca
     1021 ttcccattta tcctggctat accacgcagc tgaccataac ctcctccagt tatctggtat
     1081 tctcttttat tgtcaattat atggctgtac tggcaactcg tcagcgagcg gtagtcagac
     1141 gcattcagcg gcttgcgtat gtggacccgg tggttcatct gccaaatgtt cgcgccctga
     1201 atcgcgcgtt gcgtgatgcc ccctggtctg cgctttgtta tttacgcatc cctggcatgg
     1261 aaatgctggt taagaactat ggcatcatgc tgcggattca at
//
LOCUS       DAZSIO010000393         1297 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZSIO010000393 DAZSIO010000000
VERSION     DAZSIO010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.400"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(1..570)
                     /locus_tag="ACHX5T_004384"
                     /pseudo
     CDS             complement(1..570)
                     /locus_tag="ACHX5T_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            578..1004
                     /gene="tnpA"
                     /locus_tag="ACHX5T_004385"
                     /pseudo
     CDS             578..1004
                     /gene="tnpA"
                     /locus_tag="ACHX5T_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          348 a          323 c          284 g          342 t
ORIGIN      
        1 ggcaagttta gccacgccag catccagccc gaccattgat gctgaagggt gaaccggagt
       61 tgatacttca ctttctgcct gaatactgat gtaccactta ccgcaggact ggctgacagt
      121 gacatttttc acaacacccg tgacctgacg gctgttccgg tagcgcatcc agccaagttt
      181 tggcagaaaa atacggctgt tttcctgagc gagcttaacg ccctgcggat agcggaatgc
      241 atcattctgt caccgctttt tgaatcaggg gaacgccgag cgcttctgga aaaagttttt
      301 gcaggcctgc tcagggtctt tcagtgactg ttgcaatggc tgtgacggag catctttaat
      361 ccattgcgtt tcagtggcat ttttccactc aaccaatcag gaagccattt tcgcgtaagg
      421 gatgtctttg ttaccggcct tatgattctc attctgacgt gccagagtac gactgaaaac
      481 gaaacgacat gcgcctgcga agcgcctcat ttcacgcgcc tgttgaccac cgggtcttaa
      541 ccggaatttg aatacctgta gtcgtttcac agggcaaatt atactttggt ctgtgaaaaa
      601 aactgatatc cgacgcggaa ggcactgtgt tttcctgatg catgctcacc gggtctttgt
      661 taccaagtac cgacgccaga tttttgacca tgacgcgaca gaaaaactac gcacttactt
      721 ttcaaatgta tgtgcttatt ttgaagctga actggttgaa atggatggcg aaccagatca
      781 cgtccatctg ttaattaact atccccccaa actggcgata tccagtctgg taaacaaaca
      841 gcctcaaagg cgtatcgggt aggttactgc gacgggatcg accagatatt gctgccaggt
      901 attaccgcaa agatgttttg tggcgtcccg gctattttgc cagtagctgc ggaggtgcgc
      961 caatatccgt catccgccaa tacattgaac aacagcaaac acctggtcag atgaaaaaac
     1021 gagccttata tccccgccct gaaggacggg cttttacggt gcactggata agcctggaga
     1081 ttatctacac ctgcatggac atcattgtac tatgccccgt acatgctcca tcacatcctc
     1141 aacatcaaag tattagagcc ttgcaaacca ttatgcgtct cagtttgtct catctatctt
     1201 acgcaaaaaa aatagaacta atatcaagcg gcgctatatt gcgtattaaa taaaaccacc
     1261 tcaattacat taataaatct aaataaatat catgcaa
//
LOCUS       DAZSIO010000394         1286 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZSIO010000394 DAZSIO010000000
VERSION     DAZSIO010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.401"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..1197)
                     /locus_tag="ACHX5T_004386"
     CDS             complement(<1..1197)
                     /locus_tag="ACHX5T_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8469276.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSG"
BASE COUNT          419 a          300 c          251 g          316 t
ORIGIN      
        1 gcctgacagc cctgatgcgg ctccggcagc ttctttagga acaagctcca ttgaataaag
       61 accaataaac atcaccggac catacactgc cagccccaac agaataagga ttgtatagtt
      121 tagccagaga tattcattgc ctgatggcac gtaccagtac gcgattaata aaataatggt
      181 aagtgccaga aataaagtat ttgttaatgg ttttgagttt ctgaacagtc tgacagacag
      241 gtatccggca agaagcatac cagccacacc accgatttca aaaacagaac ccccccccat
      301 tgatgccagt tttcccatat ccttaccacc gacatccaga ctgtcactta aaaacttagg
      361 agcccaggaa acaataccgt atctcagaat ataaacaaac gaaccataga tacagcagta
      421 aatgagttta ttatttcgga tgatatgttt tctgatgata tcaggaatgg agagtctcgg
      481 accttgttca cggcctttag ccgggtgtga aacgacgtta cctttccatt cttcaatggg
      541 aggaagcccc actgatacag gtctgtccac ctgaataaag taaatcagca cactcattgc
      601 catacacagt actcctggga agataaatgc ggcccgggca tgatccagag cactgtcgcc
      661 gaaaaaacca aaactgaaac caatcagaac gggggccaga gctcccccga cattgtgcga
      721 taaattccac agtgtggccc aggtgatcct ttcccgatgt tcataccaga acaccagtgt
      781 tttggcacca ggaggatatc ccataccttg gatccagccg ttaagtaaat acattgttga
      841 aaataacgta acggatgtga tgatcccggg aatatatcct atcagaaaat ttaccagtcc
      901 ggacaggaat aaaccaagag acaaaaatac ttttgaatta cttctgtcag aaataagtcc
      961 ggaaacaaac tttgatatac cataggataa aaaaccggca caggagataa tgccgatctc
     1021 ggttgcagac atgtgcagca caccggataa cacactggtg gtgtttcgga caaaataata
     1081 ccccatatat ccgaggaaaa tgctgataaa gaccctgtat cgcaggacgg gatataatcg
     1141 ttcaatctct tcagagggta atctttcaat ttccttaggt tctgtgaata atgacatatt
     1201 taatccttct gtaatactga taaccaattg tcgcaaacct gtttacttat cattatataa
     1261 tcactcaaag tattgcatta tcacac
//
LOCUS       DAZSIO010000395         1279 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZSIO010000395 DAZSIO010000000
VERSION     DAZSIO010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.402"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..835)
                     /gene="ipaH4.5"
                     /locus_tag="ACHX5T_004387"
     CDS             complement(<1..835)
                     /gene="ipaH4.5"
                     /locus_tag="ACHX5T_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HFR8469277.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          359 a          230 c          286 g          404 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagaggtta atctttgcag ggattgcaga acgtgagtcg
       61 acaatggatt ccctgaaatg ttaacattcg tacaaatggg caatgtgatt atattttcgg
      121 gtaatgaagt tagtaggttg ttggaaacat ccagtgccaa tagttcttgc ggtaatctgg
      181 ataatctgcg tagttgattt tcacctacct tcaaatattt taagctctga ggaaattctg
      241 gtagttcctg tagtctgttg ccggaaacaa agagttcttg tagtctatga ggcaattcag
      301 gcaagacagt aaggcgatta ccttcaatac gtaatattgt tagcgttgat ggaagctctg
      361 gtaacgtctc cagactatta aaacgtgccg agagtgattg taaatatggt ggtaatgatg
      421 gcagacatga caaatttcga ttgaatgaaa tattaagctt aatcagttga gatggaagtg
      481 tcgggagtat attcaagttg ttaccattta catgaagttc tgtcaaaagc ggagaatttt
      541 caggtaggga gattaactca ttgttggaaa tattaagttc acgtatatgc aaagggagtg
      601 gtggtaaaga acgtagtttc agtaagctta aattgagaac aggctcccgg gtatccagac
      661 atattttcaa taatcgaacc gcctggatgc gattttctgt tgttgttcct tccctagccc
      721 agtcatccca gatgcggtgg taatctctgg tacactgttc ttctaccgat aaacgagata
      781 tacatgataa gccacaaagg gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt
      841 aacccctcac tattcatatc ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa
      901 aaacaatcct agagctttat cggcgttttc attccggctg gaggaatatg ttcctgtctt
      961 tccagtaacc gtcgattgtt atatcagtct acccatgtta atgtgaccag ttctactttc
     1021 atccagtttg gattgatctt tatcccctat attttcaaac aatttttatc atttaactca
     1081 ttactatctc gatattccca tagcaaacga tctggttggt tatcccctca aaaaagtcag
     1141 ttgcacgccg taaatccctg ccggtattga gcgaggattt acgggttgtt gttaaggtcc
     1201 gtaatcgggt cacagttttt ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt
     1261 gattatgaat ggtgcagtc
//
LOCUS       DAZSIO010000396         1259 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZSIO010000396 DAZSIO010000000
VERSION     DAZSIO010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.403"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(75..1019)
                     /gene="leuO"
                     /locus_tag="ACHX5T_004388"
     CDS             complement(75..1019)
                     /gene="leuO"
                     /locus_tag="ACHX5T_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR8469278.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          385 a          284 c          252 g          338 t
ORIGIN      
        1 cagaataaaa ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctggcc
       61 tattctgcat tcagttagcg tttgcaaatt gagactaatt gttcttccat ccactgatgg
      121 cctttatcgc gcccggcagc ttcatgccaa gagagataac aggttctgct gttttgtttt
      181 aacggcagcg gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc
      241 ggcgcaatag cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg
      301 ccctgatacg cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac
      361 gcgaaacgat cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac
      421 gggcctttaa ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat
      481 ggcacgctgg taaattcagg acgatggaag tcttcataac taatcacaaa ctccgtttcc
      541 tgataacgca gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata
      601 tttggcgcaa tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg
      661 ctgcaaacac aaagatgaaa tacacgttca ctgctcgcgg gttcaaaacc tgaaccaggc
      721 aattcatttt gtactagttg caatgcctgg cgaactgaac caaaaagttg aaatgcgcga
      781 gcagtcggtt gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc
      841 aggcgtgcaa cagcgttact gaccgcaggt tgcgacatac ccagaacatg agcggcacgg
      901 gtaatgtttt gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg
      961 cgtagctgtg gcttgcttaa ctccgccgtc tctgaatgat ctgtttgtac ctctggcata
     1021 cttaactcca ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct
     1081 gaagaatatg atttatcatg catataaaat aagaaaaagc aaaatgagta aaattcggaa
     1141 aacataaaga tacgggttct gataaaaaca gaatccattg ccgtggaatg agtcatttac
     1201 cacagcataa taatccataa tgttttgttg cgaaaacaat ctaatcataa ctaccgcga
//
LOCUS       DAZSIO010000397         1256 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZSIO010000397 DAZSIO010000000
VERSION     DAZSIO010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.404"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(290..>1256)
                     /locus_tag="ACHX5T_004389"
     CDS             complement(290..>1256)
                     /locus_tag="ACHX5T_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR8469279.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          377 a          275 c          270 g          334 t
ORIGIN      
        1 aatgaaccag gttaaagtgt atttaaaaat gtgttgtatt agcgactgaa atctgccttt
       61 taaaaaggag gagggtactg agacaaagag tcattgcttc tccgataaaa tgtcagagaa
      121 gcatgattac gtggttattg tctgttgatg acttgttgac cgagccgtca tcattctcaa
      181 tgcattagtt aaatcatttg tgatttttaa ggattatcct gtctatgatt ttctacttag
      241 aaggatatgg cactaactat gcatcttata gtttactgct ggcatctatt taaaatagca
      301 attgagtcaa atagcccatc aagatagcca tacctaatat aaccgtcagg aatgcagcta
      361 tcatcggcat tctgaacatt gatttaagta atatcacttc cgtcaggctt gcgccggcac
      421 tgccgatgat taatgccatt aatgctccca gacccattcc ttttgtcatc agaacagatg
      481 ccagagggat aaccgcctca gcccggatat acagcggaat accgacaacg gcgcttaatg
      541 ggatggcgaa gggattatct gctcctgcat gagcggcaat ccacgctgag ggaataaagc
      601 cataaataaa agatcctatt aatactccta acaaaagata tggcagtaca tctttaaact
      661 gttgcaacgc atctttggtg gctagtttta tgggattaat acttactaca gctttggtac
      721 tgcaacaggt aactacgggg ttctcaatgg ctttagccgc tggactacaa caactcactt
      781 ttatgggcgt atatgtcgtt cccggcgaag tttttgctgg agcacaacaa tttgctgatg
      841 agcttttact ggcaatgata tgacgttcaa accccagaga atccaggata atactggcaa
      901 gaacggagac gccggctgcg ataatcgcgt acaacaaggt gactttccaa ccaaaggtca
      961 tccacattag tccgacgata atgggattaa gtaatggtga aacaaataaa aaagtgagag
     1021 tcggaccaaa cccggctttc gctgacaaca atccacgtaa catgggtatt gtcgagcaac
     1081 tacagaacgg ggtaacggct cccaacagag cagccaggag atagcctctc ccttttctgg
     1141 cccccatcat ctgctggatt ttatggtctg gcaccttttg tcttatcagg ctgacaccgg
     1201 cactaatcac tataaacagc aacgaaagct cgacggcgag aaacacaaac atctct
//
LOCUS       DAZSIO010000398         1252 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZSIO010000398 DAZSIO010000000
VERSION     DAZSIO010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.405"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            4..906
                     /locus_tag="ACHX5T_004390"
     CDS             4..906
                     /locus_tag="ACHX5T_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8469280.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            1053..1252
                     /locus_tag="ACHX5T_004391"
                     /pseudo
     CDS             1053..1252
                     /locus_tag="ACHX5T_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT          384 a          276 c          214 g          378 t
ORIGIN      
        1 tgtatggcca atctctacga cttgaaaaag ttcgacttaa atcttcttgt catatttgag
       61 tgtatttacc agcatttaag cattagcaaa gctgctgaat cactatatat caccccatca
      121 gccgtgagcc agtcgttaca acgcttacgt gcgcaattta atgacccttt attcattcgc
      181 tcaggaaaag gtatcgcgcc gacgacaaca gggttgaatc tgcatcacca tcttgagaaa
      241 aacctcaaag gccttgaaca aacaattaat atagtcaata agtccgaact aaagaaaaac
      301 tttattatct acggccctca gctcatttct tgctctaata atagtatgct gatccgctgc
      361 ctacggcaag atgcctctgt cgagattgag tgccatgata tcctcatgtc agcagagaat
      421 gctgaagaac tgttggttca ccgcaaagcc gatctggtga tcacccaaat gccagtgatc
      481 agccgttccg tcatttgcat gccattgcat acaattcgca atactctaat ttgtagtaat
      541 aagcatccgc gaattactga caatagcact tatgaacaaa ttatggcaga agagttcacc
      601 caattaatag ccaaatcagc aggagttgat gatattcaaa tggaaataga cgagaagttt
      661 atgaatcgca aaatttcatt tcgtggttcc tctttgctga caataattaa tagcattgca
      721 gttacagatt tactgggaat agtcccctat gaactttata actaccaccg tgactttctt
      781 aatttaaaag agataaaact agaacatccg ttaccttcaa taaagcttta catttcctat
      841 aataaatctt ctttaaataa tttggtgttc tcccgattta ttgaccgtct aaatgagagt
      901 ttttgatata taactataga cagctactat aactttcatc tatttattca cagcgcgaat
      961 gaaaaataaa acactctgct ttacagtgtt tactttataa ttaacagtct tatctcactg
     1021 ttcaccctgt aacgttcaga gagcgtcttc agatgtctat ttataaaatt cctcttccgc
     1081 tcaatattct ggaagctgca cgagagcgta ttacctagac actcaacacc ctaccccgcg
     1141 tttgtgtctc attttctggc ggcaaagact ctggtttaat gctgcatctg acagccgaac
     1201 ttgcccgaca aatgggcaaa aaaatctgcg ttttgtttat caactgggag gc
//
LOCUS       DAZSIO010000399         1248 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZSIO010000399 DAZSIO010000000
VERSION     DAZSIO010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.406"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="ACHX5T_004392"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="ACHX5T_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR8469281.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
BASE COUNT          370 a          228 c          258 g          392 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagag
//
LOCUS       DAZSIO010000400         1243 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZSIO010000400 DAZSIO010000000
VERSION     DAZSIO010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.407"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            110..1243
                     /locus_tag="ACHX5T_004393"
                     /pseudo
     CDS             110..1243
                     /locus_tag="ACHX5T_004393"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          346 a          229 c          323 g          345 t
ORIGIN      
        1 actgtcatta attttgataa tggccttact tgcgggtcag ttaagtgccc aggagtggtc
       61 atttgattct tcacagttgg aaggaaacgt tagtgcggac actgtggcaa tgtttaatca
      121 gggagagcaa ctccccggaa attatcgtgt ggaaatttat cttaacggtg aaaaagttga
      181 tgttggggag tttccctttc atcgtccgga atcccctgaa gaaaaagagc ttgttccatg
      241 tttaaccgtt gatgacctta tccactatgg cattaaaatt gataaaagca gcagtgatac
      301 tgacaataaa aagaatcagt gttttaaatg gaactctatt gaaggtttga aagtaaatta
      361 tgattttgat agccagcgcg tacagataac cgttccacaa ctgtatctgc aagataaaaa
      421 aagtagttta gcaccagtgt cgttatggaa tgagggtgta gctgcattcc gcatggtgta
      481 tcagacaaat attgatattt ccaaacaaaa tgataatcaa tctaccacgc gtaattctcg
      541 ttatggcagg ttcactccag ggtttaatct tggtgcctgg aggtttcgta gttcagtgac
      601 atggagcaag gaacttggcc agtcagaaag atggcaaagg ggttacatgt ggtttgagcg
      661 aggcataaat gcgattaaaa gccgattaac gcttggagaa tcttatacgt cgtctgaggt
      721 gtttgacagc ataccttttc gcggcgggat gttagcgacc gacgatgcga tgactccgcc
      781 ggaggacagc tactacacac ctgtggtgca tggaatagca caaagtgagg cgcaggttat
      841 tattaaacaa aatgggcaaa ttatttttac ccgttctgtg ccgccagggc cttttgcgct
      901 ggataattta ccaacgctgg ccgttggcgg ggagcttgat gttacagtga gagagagtaa
      961 tggcgaggag cagtattttt ccgtgccatt tcaaacgccg gccatcgctc tgcatgaagg
     1021 atatttcaaa tatagtgtga tgggggggaa tataaagaag aaggtataaa aagctcccct
     1081 gtctccgtgg gcgaaatcac ggcgatgtac gggcttcctt atggcgtcac ggtgtatgga
     1141 gggattcaaa acgccactca ttttaatgca atatctacgg gtattggcat ttctctgggg
     1201 ctgttaggga gtttatcgac agatattacg cgttcgattg cga
//
LOCUS       DAZSIO010000401         1243 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZSIO010000401 DAZSIO010000000
VERSION     DAZSIO010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.408"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            188..367
                     /locus_tag="ACHX5T_004394"
                     /pseudo
     CDS             188..367
                     /locus_tag="ACHX5T_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(384..1074)
                     /locus_tag="ACHX5T_004395"
                     /pseudo
     CDS             complement(384..1074)
                     /locus_tag="ACHX5T_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1153..1243
                     /locus_tag="ACHX5T_004396"
                     /pseudo
     CDS             1153..1243
                     /locus_tag="ACHX5T_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          339 a          269 c          307 g          328 t
ORIGIN      
        1 ggttttctat ggaattcttt ttttattgtg gctgcagtca ataataacac tgaattcaac
       61 attccagtga gcaaggaaat gtaagccgag taagtatatt tgttttacat catattttag
      121 tcaagctcat ttattcctat tatagaatga caaattaaaa ttactgtaaa aaaacgatac
      181 acatttaata cgagggaggc cggcagaaaa acggcttgcg gtcaggcagg agaaatgtgt
      241 gccgcgaatg aagtccttgt atgactggat aaagctccag aggaaaactt tgtcaaacca
      301 tgctgaaatg gtaaaggcgt tcaactatat cctgaatcac tggaatgcac tgaatgagtt
      361 ttgccgggta gtacttccaa tcattagaat atgtgctttt caatgaagga accgatgact
      421 ttttcgtgga tttccacgga acgcgagaag cagattgttt tacgtgccag ccgcttgatg
      481 cgggggcgga gggtcaggtt gttacgttca tacgttgcgt gaagattttg ccggtcaggt
      541 gtttctcctc cggcacttct ctggcgtagc tgccccagtc atcgctggtc accatgccga
      601 tacagaacgg agtcaacagg gctaacagtt cccgacaggt ctcatcattc cgtggcccgc
      661 aagtgtaagc caacacaccg ccagttttag tgttgtacgc ataccagggc cagtgttgtt
      721 gcgccttgct gccaacgaag cgccactgct catcaagttc acagataagg gcaacatcag
      781 cgtgagcgac tggcgcagat gttatccgcc ggggcgagag ctttttaatg tccgtatgac
      841 ggtattgatg ccgattttca gtgtcctggc ggtatcacgg ccccctgcgc cgttatgtgc
      901 catttcaacg atctgctcct taacgccgag ttttctggct tcataagagt aagtgagctg
      961 gaaaacacgt ttacatgagc gacaacggaa acgctcatgc cgggaacaac tacggccatg
     1021 tcgataaact tcatcggaat tacagcgcgg acaatgaaca gtaacggtag tcatggaaaa
     1081 cctcaaatac cagtagatta ccagaaactt ctatcgattg aagacattac cttttgccgc
     1141 aataggtggg gtaacattgg tgaaaacgca ttacgccggg tggcagttgg aaaaaaatta
     1201 tcttttttcg gctctgatcg tggtggagaa aatgcggcaa taa
//
LOCUS       DAZSIO010000402         1228 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZSIO010000402 DAZSIO010000000
VERSION     DAZSIO010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.409"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..128
                     /locus_tag="ACHX5T_004397"
     CDS             <1..128
                     /locus_tag="ACHX5T_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469282.1"
                     /translation="DLASMGQLVKLPTRRGRAFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(167..970)
                     /gene="dkgB"
                     /locus_tag="ACHX5T_004398"
     CDS             complement(167..970)
                     /gene="dkgB"
                     /locus_tag="ACHX5T_004398"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR8469283.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(1133..1209)
                     /locus_tag="ACHX5T_004399"
     tRNA            complement(1133..1209)
                     /locus_tag="ACHX5T_004399"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1173..1175),aa:Asp,seq:gtc)
BASE COUNT          326 a          289 c          288 g          325 t
ORIGIN      
        1 gcgatcttgc aagtatggga caacttgtga aattaccgac gagaagggga agggccttcc
       61 cgagagtggt aaaggagagg ccctggaaat accccacagc cccgaaaaag agccagtcag
      121 ttgcttaact gactggcatt actcccggag gagctgtcag agaggcttaa tcccattcag
      181 gagccagacc ttccgggcta accaggcggt cgttgcaatc cagtgcggcg atcgcttttt
      241 tatcttcggc atcaagctgt aaattttgtg ccttaagatt actttccagg tttttacgtt
      301 tagtagaaga aggaattact gagtaacctt cccccatagc ccacgccaga atcacttgtg
      361 ccggaatcgc attgtgttta gctgcaatac gagcaataac ctcatctttc agtgccttac
      421 cataagccag cgtcatatag gaagtgatat gaataccgtg ctgtttagcc caggcaacca
      481 ctttacggtt ttgcagataa ggagagagtt caatctggtt ggtagcgatg ttttcagcgc
      541 caacagcagc aatcactttt tccatcaatg ggatcgtgaa gttggaaata ccgatctcac
      601 gcgtcagccc ttgttttttg gcttccagca gcgcctgcat aaactcttca acagagactt
      661 catcgtttgg tgacggccag tggattagcg tcagatcaac ataatcggta cgcaattttt
      721 gcaggctctc tttcagactc gggatcaatt tgtctttgct gagattttca atccagattt
      781 tagtggtgat gtagagttca tgacgtggca cgccactttc tgcaatcgcc tgacctactg
      841 cggcttcgtt atcatagatt tgtgcggtat caattgcgcg ataaccaagt tcaagcgccg
      901 ttttcacaga tgaaataaca acgtcgtctt tcagacggaa agtacctaaa ccaaatgcag
      961 ggatagccat aatatgcctc ttttaatgct attttcgtta actgaggtga ttatgggctt
     1021 cagactgttg cgggaaaaga gcgtaaaacg cagaggattt ttgcgattct agcaataata
     1081 gatatccaga cgcaaaaaaa catcgagcgt taactcgagg cttcttaata agtggcggaa
     1141 cggacgggac tcgaacccgc gaccccctgc gtgacaggca ggtattctaa ccgactgaac
     1201 taccgctcca ccgaattctt ttacaacc
//
LOCUS       DAZSIO010000403         1221 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZSIO010000403 DAZSIO010000000
VERSION     DAZSIO010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.410"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..141
                     /locus_tag="ACHX5T_004400"
     CDS             <1..141
                     /locus_tag="ACHX5T_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8469284.1"
                     /translation="RNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRL
                     RN"
     gene            complement(178..309)
                     /locus_tag="ACHX5T_004401"
     CDS             complement(178..309)
                     /locus_tag="ACHX5T_004401"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469285.1"
                     /translation="MARSAKSRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFD"
     gene            391..584
                     /locus_tag="ACHX5T_004402"
                     /pseudo
     CDS             391..584
                     /locus_tag="ACHX5T_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
     gene            727..948
                     /locus_tag="ACHX5T_004403"
                     /pseudo
     CDS             727..948
                     /locus_tag="ACHX5T_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(969..1031)
                     /locus_tag="ACHX5T_004404"
                     /pseudo
     CDS             complement(969..1031)
                     /locus_tag="ACHX5T_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          360 a          289 c          287 g          285 t
ORIGIN      
        1 cgtaatgtcg ttgaacggtg ctttgggcgg ctgaaagaat atcgccgcat cgccacgcgt
       61 tacgacaaaa cggcaagaaa ttacctggcg atggtgaaac tgggctgcat ccgactcttt
      121 tatcaacgct tacgtaatta agggacacag cctagtctcc aggattcccg gggcggttca
      181 atcaaagcgc agaacttctg cgtcttcttc gtcaaaaaaa tcatcttcgt gaagcttcac
      241 gacatagcgg ggaagtttgc ttctttgaga ggcgggttta cgtttacggg atttagctga
      301 acgggccata taaccacctg aaagacaatg acattgcctg tttttataac ggtaattgca
      361 gaccatgaca aaccgcagcc gtcaggctgc ctactcgagt cacaaggctc tttgtgtccg
      421 atatgccggc agcgcattac ccatgaaact ggctggaacg tacaccataa ggtgaagaaa
      481 gttatggggg cggtgaagaa ttatctaacc ttgtcttgtt acatccaaat tgccatcgac
      541 agttacacag ttgtgaagcc ggctctctca aaaagggctt ataaaggctt gagcggtgta
      601 ccgggaaacc ggtatgcacc gttcttaggg gagggcagtc cagcaatgaa ctgtccttac
      661 ccgacaaaca tccaaaacga acggaacgtg ctggaatccg catataaccc gttgtaaagt
      721 gaggaaatgg acacctcact tgctcatgag aacgcccgcc tgcgggcact gttgcagacg
      781 caacaggaca ctatccacca gatggctgaa tacaaccgcc tgctctcaca gcggatggcg
      841 gcttatgctt ccgaaatcaa ccgactgaag gcgctggttg cgaaactgca gcgtatgcag
      901 ttcggtaaaa gctcagaaaa acttcgcgca aaaaccgaac ggcagattcc attttcacgg
      961 gcgatctacg ccacacaggc tctgggttgc agtgactgct ccattaaggc gattttgaac
     1021 tcataaggga agtaacaaaa aaactgcaaa agtacaataa ttatatatgg ccaaactaac
     1081 ctctattaaa aaacatcagt ttttcacact tagttgagct tcatgaaaaa atcatcgagg
     1141 atttcattgc agaatacaca ttccactaac tggaagcact ccataagagg aatcagagaa
     1201 agtatcgttt ttttatagta a
//
LOCUS       DAZSIO010000404         1218 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZSIO010000404 DAZSIO010000000
VERSION     DAZSIO010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.411"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..68
                     /locus_tag="ACHX5T_004405"
     CDS             <1..68
                     /locus_tag="ACHX5T_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469286.1"
                     /translation="EQIEPNLLGVYAGLLLAMKNK"
     gene            complement(104..>1218)
                     /locus_tag="ACHX5T_004406"
     CDS             complement(104..>1218)
                     /locus_tag="ACHX5T_004406"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFR8469287.1"
                     /translation="PIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIKNPGYSNYTFV
                     NDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESVIVVHNSMKLY
                     TQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANADIEIVPNGI
                     DLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAAAHKNLKLV
                     VIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPLADLVVIPSQF
                     QEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDILKTLAN
                     PELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          384 a          241 c          206 g          387 t
ORIGIN      
        1 tagaacaaat tgaaccaaat ttattaggcg tttacgccgg cttattatta gcgatgaaaa
       61 acaagtaaaa aataaaaaag gctgcataat gcggcctttt tatttattca aaccaattat
      121 gaataacctc ttcgaatctt tgagttacgc catcccaact gtagtgatca aacacaaagt
      181 cctgaccttg ctttgcaact gctgttaatt caggattcgc taacgttttt aaaatatcgc
      241 tgcttatgga gtctgcagtc ataggttctt ttaaatggaa tcccgtagta ttttctttga
      301 caaattccgt catcccccca cgcgtactca ctaaaacagg cttaccagca cccatagctt
      361 caattgccac catgcaaaat ggttcctgga actgggaagg aatgacgact aaatctgcca
      421 gtgggtaata acaatacatc ttttcaggcg gtatgcttcc taacataatg caacgatcct
      481 tcaaacgttt tgcaatttcc ctgacattgc gttgatatgc acctttgtca cttttactca
      541 tttctgtata atcaccaatc acaactaatt ttaaattttt gtgagcagcg gccaattttt
      601 cgaatgattg catcaacagc aatatgcctt tgtcagggac gatacgccca gcataaaaaa
      661 taattttctc ttcaggagag atattaagtt cgcttctagt gatcggctga aaattagatt
      721 gataggtttc taaatcaatt ccatttggta caatctcaat atctgcattt gctaaataag
      781 attggtaata ctttttcaag tataaactag ggactatcat tttaacattt tgctcaagca
      841 attttggttc aaatgcatta tgcatatgta taaccactct tgcctgagga gctagcttac
      901 gaatttgagt gtatagcttc atgctattat gaactacaat aacactttct ttcgttatag
      961 gaaaatcatg tgcaatattc aatattctct gtgaatatgg aagaggatca agtcttgtcc
     1021 atttttgaaa taaccgctta tatatgcgac tgaatccaac cctatgaata ctacaattat
     1081 cattaacaaa ggtgtaattt gagtatcctg gatttttgat acaaacaata cgatttggaa
     1141 aattggtacg ctgagcaacc tgatacatcc acgtttcaac agcagccgct ccgcgaggag
     1201 gaattgaaaa aataggag
//
LOCUS       DAZSIO010000405         1215 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZSIO010000405 DAZSIO010000000
VERSION     DAZSIO010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.412"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..1103)
                     /locus_tag="ACHX5T_004407"
     CDS             complement(<1..1103)
                     /locus_tag="ACHX5T_004407"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR8469288.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICT"
BASE COUNT          300 a          313 c          309 g          293 t
ORIGIN      
        1 gtacagatga actcatacgg ccccagtttg gcgatctcat ccgccagata ggcagcgacc
       61 tggtaggaag cattctgtac tttggtatag ccttcacgac caaggcgcag gaattcatag
      121 tactgtgcaa tcacctgacc cgccgggcgg gagaagttga tggcaaaagt accaatctga
      181 ccaccgaggt agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc gtcacgccag
      241 ataacccagc cgcagcccag cggagccaga ccgaatttat ggcctgaagc actgatcgat
      301 ttcacacgcg gcaggcggaa gtcccagacg atatccgggg cgacgaacgg tgccagaaag
      361 ccaccgctgg cggcgtcgat gtgcatgtcg atgtcgatac cggtgtcggc ctggaattta
      421 tccagtgcat cgtgcagcgg ttgcgggaac tcatagttac cggtgtaggt cacgccgaaa
      481 gtcggcacca cgccgatggt attttcgtcg caggcttcaa tcatgcgttt cgggtccata
      541 aacaactgac cggggcgcat agggatctca cgcagctcca catcccagta gcgggcgaat
      601 ttatgccagc agatttgcac cggaccgcac accaggtttg gtttatccgt tggtttgcct
      661 gcagcttcca tacgcttgcg ccaacgccat ttcatcgcca tcccgccgag catacaggcc
      721 tcggaagaac caatggtgtt ggtgccaacg gcctgaccat ttttcggcgc aggcgcatgc
      781 cacagatcgg caaccatgtt tacgcaacgc aggtcgatgg ctgcggattg cggatattct
      841 tctttgtcga tccagttttt gttaatggat aaatccatca acttgtggac gttttcgtcg
      901 tcccaggtct ggcagaaagt ggccaggttc tgacgagcgt tgccatcaag atataattca
      961 tcattgataa tctgaaatgc gacatcgtcg cgcatttcgt gcagcggaaa acgttttgat
     1021 tctgcgatag tggaaataga cttcgcacca aaacgtgaat cgagtagttc cgaccttaaa
     1081 tccgttactt gcttcttatc cattttaaac tccttaaaat gattggatcg cattaaaaaa
     1141 gtaggattta tcgataaagt aagcaagtgc aaaggactcg tgtttaaata acaaaatcct
     1201 aatgttattt atcgt
//
LOCUS       DAZSIO010000406         1212 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZSIO010000406 DAZSIO010000000
VERSION     DAZSIO010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.413"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..143)
                     /locus_tag="ACHX5T_004408"
     CDS             complement(<1..143)
                     /locus_tag="ACHX5T_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFR8469289.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFT"
     gene            complement(545..>1212)
                     /gene="ydeO"
                     /locus_tag="ACHX5T_004409"
     CDS             complement(545..>1212)
                     /gene="ydeO"
                     /locus_tag="ACHX5T_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR8469290.1"
                     /translation="VDNAVRTHFEPYERHFKEIGFNENTIKKYLQCTNIQTVTVPVPA
                     KFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFAACKGFITLLTNGVLS
                     VSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQTTFSQILLDARMQHAKN
                     LIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSA
                     LAI"
BASE COUNT          379 a          227 c          227 g          379 t
ORIGIN      
        1 gtaaagtctg agaaagcaac gtttgttttg gttgctttca gctttacatc atctgccgca
       61 tgagcagcaa atgcagccat accagatgcc agtatcaaag atatcgaggt acttacgaca
      121 cttttcttta atgcagactt cataaatgtt cctcttctta ttgtacttaa ttccgaagta
      181 attttacgaa gagaaataag tggatgtaag tgaagttagt cacataaaga gatagcagat
      241 ttagctaaaa aaagggaaat aacagtccat aaagcgttga cattactttc tgttctatta
      301 agtaatttct cgccgataaa caactaattt attgatattt aataaattat tgcattttac
      361 tgacaaaatg ccgaattgag atcataaata atcatgcaac aggttatgca agtgcataaa
      421 tatgtgatgg atgtcactta tttatttcaa taaatatatc gcctaaaaac aacgcggggc
      481 agggaatggc tgccccatat aattcttacg tagcgtgcgt ggttgactac tcgttagcaa
      541 ataatcaaat agctaaagca ctcatcgtgt tgcccgtatt catacccgtg tgcctttgac
      601 aacggtactc cttagaaact ctcttcggac tgttgccgaa atgtttgcgg aacgcataaa
      661 taaaataaga tgtactggca taaccacatt gttcggcaat tttattgact gaaccttcta
      721 cgcgtatcaa attttttgcg tgctgcattc tggcatctaa tagaatctgt gagaatgtcg
      781 tttgctcttg ctttagtttt ttcttcaaca ggctttcact gatgtacagg cagtcacaaa
      841 tatctttcag cttccatggg tgcgccagct tcatgttgac aatatttctc actttcccgg
      901 aaacggatag cacaccgtta gttaatagtg taatgaaacc tttgcatgcg gcaaaaatag
      961 acaggcaaga aaaaagcaaa agttctgaaa aattatgatg attgcgttct tccgagttga
     1021 gataagcaat catttcatta agcaatccag tcggtacatt tgaagcacgt aaaaacttcg
     1081 caggaacagg caccgtcact gtctggatgt tagtgcattg tagatatttt ttaatggtat
     1141 tttcattaaa tccgatttct ttaaaatgcc gctcataagg ttcaaaatgt gtacgaacag
     1201 cgttatctac ca
//
LOCUS       DAZSIO010000407         1193 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZSIO010000407 DAZSIO010000000
VERSION     DAZSIO010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.414"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..815)
                     /locus_tag="ACHX5T_004410"
     CDS             complement(<1..815)
                     /locus_tag="ACHX5T_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8469291.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="ACHX5T_004411"
                     /pseudo
     CDS             907..993
                     /locus_tag="ACHX5T_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1003..1083)
                     /locus_tag="ACHX5T_004412"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="ACHX5T_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          336 a          208 c          319 g          330 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agt
//
LOCUS       DAZSIO010000408         1192 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZSIO010000408 DAZSIO010000000
VERSION     DAZSIO010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.415"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            31..780
                     /gene="phoC"
                     /locus_tag="ACHX5T_004413"
     CDS             31..780
                     /gene="phoC"
                     /locus_tag="ACHX5T_004413"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase PhoC"
                     /protein_id="HFR8469292.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(835..939)
                     /locus_tag="ACHX5T_004414"
                     /pseudo
     CDS             complement(835..939)
                     /locus_tag="ACHX5T_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(950..>1192)
                     /locus_tag="ACHX5T_004415"
     CDS             complement(950..>1192)
                     /locus_tag="ACHX5T_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8469293.1"
                     /translation="ELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGR
                     LKEYRRIATRYDKTARNYLAIVKLGCIRLFYQRLRN"
BASE COUNT          319 a          267 c          259 g          347 t
ORIGIN      
        1 aaatatgtat cctctataag gagatgttat atgaaaagac agctttttac tcttagtatt
       61 gtcggggtat tttctttaaa tacctttgca tcattccctc cgggaaatga tgtgacaaca
      121 aaacctgacc tttactacct gacaaacgat aatgctattg acagtctggc attattaccg
      181 cctccaccac aaatcggaag tattgccttc ctgaatgacc aggccatgta tgaaaagggg
      241 cgtttattgc gaaatactga aagagggaag ctggcagctg aggatgccaa tctcagtagt
      301 ggtggcgtgg caaatgtatt ttctgctgct tttggttctc cgataacggc taaagattca
      361 ccagaattgc ataaattact gacaaatatg attgaggatg ccggagacct ggcaacgcgt
      421 tcagcaaaag aatattatat gcgtattcga ccttttgctt tttatggtgt ttctacgtgt
      481 aatacaaaag aacaggatac attatccaga aatggctctt atccatcagg tcatacatcg
      541 attggttggg caacagcact tgtattgtcc gaaataaatc cggcacgcca ggataccatt
      601 ctgaaacggg gttatgaact gggggacagc agggttattt gcggttatca ctggcaaagt
      661 gatgttgacg cagcacgtat tgttggctct gctattgtag caacactgca ctcaaatcct
      721 gtgttccagg cacaattaca aaaagcgaaa gatgaattcg ctaacaatca gaaaaaataa
      781 tgcttttagc cagtacgcct tataaccccg accttcaggg ctaatgccag tcagttaagc
      841 aactgactgg ctctttttca gggctgtggg gtatttccag tgcctctcct ttaccactct
      901 cggaaaggcc ctttcccttc ttgtcggtaa tttcactagg ctgtgtccct taattacgta
      961 agcgttgata aaagagtcgg atgcagccca gtttcactat cgccaggtaa tttctcgccg
     1021 ttttgtcgta acgcgtggcg atgcggcgat attctttcag ccgcccaaag caccgttcaa
     1081 cgacattacg attgcagtac gcatcacgat caagctgtgc acgtccatcc gatgccattt
     1141 tctcatttga ctttcggggg attactgcct ttataccgtt atttttcagc tc
//
LOCUS       DAZSIO010000409         1188 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZSIO010000409 DAZSIO010000000
VERSION     DAZSIO010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.416"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..823
                     /gene="virA"
                     /locus_tag="ACHX5T_004416"
     CDS             <1..823
                     /gene="virA"
                     /locus_tag="ACHX5T_004416"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFR8469294.1"
                     /translation="QFNLGKVHTITAPVSGNFKTHKPAPEVIETAINCCTSIIPNDDY
                     FHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINELGINEFIDSKK
                     ELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTSAFWQSLHITSS
                     SVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAISASGGRAYEVS
                     SSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVSFAIISPDV"
     gene            1002..>1188
                     /locus_tag="ACHX5T_004417"
     CDS             1002..>1188
                     /locus_tag="ACHX5T_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8469295.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT          404 a          250 c          211 g          323 t
ORIGIN      
        1 acaattcaac ttaggaaaag tacatactat tacagctccg gtatcaggga attttaaaac
       61 tcacaagcct gcaccagaag ttattgaaac agccattaat tgctgtacat cgataatacc
      121 aaacgatgat tacttccacg ttaaagacac tgattttaat agtgtttggc atgatattta
      181 cagagatatt cgagcatctg acagcaatag tacaaaaata tatttcaaca atattgaaat
      241 accactaaaa ttaattgcag atctaattaa tgagttagga attaatgaat tcattgatag
      301 caaaaaagag cttcaaatgc taagttacaa ccaagtaaat aaaataatca attcaaactt
      361 cccccaacaa gacctatgtt ttcaaacaga aaaacttttg tttacttcac tctttcaaga
      421 ccctgcattt atatctgcac taacatctgc attctggcaa tctcttcaca tcacgtcttc
      481 ctctgtggaa cacatatatg cccaaattat gtccgaaaac atagaaaaca ggcttaattt
      541 catgcctgaa caacgagtta ttaacaattg tggacatatt ataaaaatca acgcagttgt
      601 ccctaaaaac gacactgcaa tctctgcctc tggggggagg gcttacgaag ttagctcatc
      661 aatattacca tcgcacataa catgtaatgg tgttggtatt aataaaatag aaacaagcta
      721 tctcgttcat gctggaacac ttccaagcag tgaagggctt cgcaatgcta tccctccaga
      781 aagtcgtcaa gtatcatttg ccatcatatc tcctgatgtt taagccaata ttatattggc
      841 tgccagactg taaaaaaacg ttcttcagaa cagctctata aactattaaa ccaaagctaa
      901 aaggatgggg accaatagaa tctcagccca tcctttttat atcattatga accgccccgg
      961 aaatcctgga gactaaactc cctgagaaag aggtaaacag gatgactaaa aatacacgtt
     1021 tttcccctga agtccgtcaa cgggcagttc gtatggttct ggaaagtcag ggcgaatatg
     1081 actcacaatg ggcagcgatt tgttccattg ccccaaagat tggctgtata ctggaaactc
     1141 tgcgtgtctg ggtacgccag catgagcggg ataccggggg cggtgagg
//
LOCUS       DAZSIO010000410         1159 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZSIO010000410 DAZSIO010000000
VERSION     DAZSIO010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.417"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            115..>1159
                     /gene="cadB"
                     /locus_tag="ACHX5T_004418"
     CDS             115..>1159
                     /gene="cadB"
                     /locus_tag="ACHX5T_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR8469296.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKAS"
BASE COUNT          246 a          255 c          298 g          360 t
ORIGIN      
        1 tttaggatta atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat
       61 agttatacat catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt
      121 tctgccaaga agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg
      181 agcggtattg cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt
      241 tggattatct ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca
      301 aaaaacccgc aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt
      361 tttcagacag gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt
      421 accgctgtat cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt
      481 atcgcctgta ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg
      541 gtaagccgtt taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct
      601 attgttggct ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat
      661 accactgatg gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt
      721 gttgaatccg cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg
      781 gcaaccatgc tgggtactgg tttagcaggt attgtttata tcgctgcgac tcaggtgctt
      841 tccggtatgt atccgtcttc tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct
      901 tcaactatcc tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc
      961 ctgacttctc tgggctcctg gatgatgttg gtaggccagg caggtgtacg tgccgctaac
     1021 gacggtaact tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt
     1081 ctgctgctgg ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct
     1141 gccggtggta aagcatctg
//
LOCUS       DAZSIO010000411         1155 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZSIO010000411 DAZSIO010000000
VERSION     DAZSIO010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.418"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            47..>1155
                     /locus_tag="ACHX5T_004419"
     CDS             47..>1155
                     /locus_tag="ACHX5T_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR8469297.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNI"
BASE COUNT          392 a          170 c          221 g          372 t
ORIGIN      
        1 cgcgaggagt tcggtggatt atcgtttttc ttgggtgatt tttaatatga taaacaatat
       61 atctgatcaa gcatcatcat ttccaggcac acaactaaat caaagtgata atttcctgga
      121 ttcactgagg gaatttttcg cgatattaaa tcccagtcgt aagggtgagt tgtctacatg
      181 ggataccatc tacttgcatc tgatattagc aataaatgct gacagtgatc tcataaaaaa
      241 tgatgtgttg ctcgcggaaa atattccatc agcgaattac caatttaata cattttttag
      301 caatactttc gagattgatg ttaaaaaata tctgggtaaa tcagaagata acgaagttga
      361 aataaaagcg ggtaatgaaa gaatctctat tggaatacgt aatgtatcga atggaaaact
      421 tgaaagacaa cagtttcttt tcccactgga ctatgagaat aaattacaag aacaattaga
      481 taagtatttt acgattgaat cgcacccatt attatacagg tataccattg gtagtaagat
      541 tgcgaatgtg atttttgaaa agttatacag tcgtattgac tttaacaaag aacaatatat
      601 atcctttata aaggacgcat ttattcattt ttatgattat agcaggcgat atgctattag
      661 tgagaatatc gataaagacg ctgtaacaaa caatatcgca ttgatgagca ctttttatga
      721 cagtgataat acgtcaggtg aggttttaaa taatgatttt acagaagaag agtcatttga
      781 aactgcgtta gatgtagagc atgcaatagt gcttggattt gctgatgata attttgaaac
      841 aaaacctgtt cattatcagg atttattaac acgatttagt gcctttcaag ataccgtgtt
      901 taatctgttt cctgaaatgc actcttcgca ttaccacgat atttgtagcg taagtgtgga
      961 tatgacaaag ggaacgcaat gcatgattca cttgatggtg aatgaagaag ttttcatgtc
     1021 tctgcctgtt ccagttgcca cgatggtacg tgaagatgca agtaatttag tcaatcttaa
     1081 aacactactc aatgatggtt gtttcattaa atatagtcat tttaatgacg tggcattaat
     1141 aaagcaaaac ataag
//
LOCUS       DAZSIO010000412         1144 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZSIO010000412 DAZSIO010000000
VERSION     DAZSIO010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.419"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(128..>1144)
                     /locus_tag="ACHX5T_004420"
     CDS             complement(128..>1144)
                     /locus_tag="ACHX5T_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR8469298.1"
                     /translation="GNKLDLYGKVTALRYFTDDKRDDGDKTYARLGFKGETQINDQMI
                     GFGHWEYDFKGYNDEANGSRGNKTRLAYAGLKISEFGSLDYGRNYGVGYDIGSWTDML
                     PEFGGDTWSQKDVFMTYRTTGLATYRNYDFFGLIEGLNFAAQYQGKNKRTDNSHLYGA
                     DDTRANGDGFGISSTYVYDGFGIGAVYTKSDRTNAQERAAANPLNASGKNAELWATGI
                     KYDANNIYFAANYDETLNMTTYGDGYISNKAQSFEVVAQYQFDFGLRPSLAYLKSKGR
                     DLGRYADQDMIEYIDVGATYFFNKNMSTYVDYKINLIDESDFTRAVDIRTDNIVATGI
                     TYQF"
BASE COUNT          320 a          252 c          252 g          320 t
ORIGIN      
        1 atatggaata atattaattg aggtgaatga ccagaaatat caagaattgc tcatttttac
       61 ttaaggagtc atacagcagc acttaaatca aaaaccagcc cagagaaggg ctggtcagga
      121 gaggacgtta gaactgatag gtaatgcccg tagcgacgat gttatcggtg cgaatatcta
      181 cggcacgggt aaagtcgctt tcatcaatca ggttgatttt ataatcaaca taggtcgaca
      241 tatttttgtt gaagaaatac gtcgcaccaa cgtcgatata ctcaatcatg tcctggtcgg
      301 cgtagcggcc cagatctctg cctttcgatt tcaggtaagc gagtgatggg cgcaagccga
      361 agtcgaattg atattgcgcc accacttcaa aactttgtgc tttgttagag atataaccat
      421 ccccataggt ggtcatgttt aatgtttcat cgtaattagc tgcaaagtag atgttgttgg
      481 catcatactt tatacctgtg gcccatagtt ctgcattctt accggaggca ttgagaggat
      541 tagctgcggc tctttcctgc gcatttgtcc gatcggattt ggtatatacc gcaccgatac
      601 caaagccatc ataaacataa gttgaggaga taccgaaacc gtcaccgttg gcacgcgtgt
      661 cgtcagcacc ataaagatga ctgttgtcag tacgtttatt tttgccttga tattgcgcgg
      721 caaagttcag cccttcaatt aagccaaaga aatcgtagtt gcgataggtt gctagaccgg
      781 tggtacggta tgtcatgaag acatctttct gactccaggt atcgccacca aattctggca
      841 acatatcggt ccatgaacca atgtcgtagc cgacaccgta gttacgacca tagtccagag
      901 agccaaattc actaattttt aaaccagcat aggccagacg ggtcttgttg ccgcgcgagc
      961 cgttggcttc atcgttatag cctttaaaat catattccca gtgaccaaaa ccaatcattt
     1021 gatcattgat ttgcgtttct cctttaaagc cgagacgggc ataagtttta tcaccatcgt
     1081 cacgcttatc atcagtaaaa taacgtagag cggtaacctt gccataaagg tcgagtttat
     1141 tacc
//
LOCUS       DAZSIO010000413         1142 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZSIO010000413 DAZSIO010000000
VERSION     DAZSIO010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.420"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..732
                     /locus_tag="ACHX5T_004421"
     CDS             <1..732
                     /locus_tag="ACHX5T_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFR8469299.1"
                     /translation="KTDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMV
                     NKPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITF
                     TGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASG
                     VHNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDR
                     PLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(734..960)
                     /locus_tag="ACHX5T_004422"
                     /pseudo
     CDS             complement(734..960)
                     /locus_tag="ACHX5T_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          300 a          283 c          247 g          312 t
ORIGIN      
        1 aaaaccgaca gaatccattt gaaagacgca gcgcgaatgc tggaagtttc tgtaatgact
       61 attcgtcgcg atctccatca ggaagatgaa cctctgccac tgaccctact gggtggctat
      121 attgtaatgg tgaataaacc cgcgccatcc atgccagtaa tccatgacgt tccaaaaaat
      181 catcgtgatg acttacctat tgcaattctg gctgccggaa tggttaatga aaatgatctg
      241 atcttctttg ataatggcca ggagatacca ctcgttataa gcatgatccc ggatgcaatc
      301 accttcaccg gcatctgtta ctcacatcgc gtctttgttg cgttgaatga aaagcctaat
      361 gtaacagcaa tactttgtgg tggtacgtat cgtgccagaa gtgatgcttt ttacgatgcc
      421 agtaactctt cgccattaga ctctctcaat ccgcgaaaaa tatttatttc cgccagcggt
      481 gtgcataatc actttggcgt cagctggttt aaccctgaag atcttgccac taagcgtaaa
      541 gtgatgaacc gtggactacg gaaaattttg ctcgcccgcc acgcgttgtt cgatgaagtg
      601 gcctctgcca gcctcgcacc gatctctgca tttgacgttc tgattagcga tcgtccgtta
      661 ccggcagatt atgttacgca ctgccagaat ggttctgtaa agatcattac acctgattca
      721 gaagacgaat gacttactga aaaaacacca caatcttgtt aaacatcgtc gggttggact
      781 gattacgttg cactttcacc acatattcca gcttatctat ttggcttatc acctactcca
      841 gacgctggtc atccttgacc agtagccaga tatggctttt gtcgcagtcc tgaatcggca
      901 ggcagagaat gtcttcaacg ttaagagcgc gacgggcaaa agcccacaaa ggtgagtcat
      961 tacacccgga tggtaaacag gcacttcctt gttgtagaag ccgttatatt gtccataatg
     1021 tctgggtttt taatgcatac tgggtaacaa ccggacgaat cttcgaatat cactggcgct
     1081 gccagccggt ttgaattcat ctcacaaccc tgcatagagc gaatctcctg cctgcacgtc
     1141 ac
//
LOCUS       DAZSIO010000414         1128 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZSIO010000414 DAZSIO010000000
VERSION     DAZSIO010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.421"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..96)
                     /locus_tag="ACHX5T_004423"
     CDS             complement(<1..96)
                     /locus_tag="ACHX5T_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8469300.1"
                     /translation="MSRKTQRYSTEFKAEAVKTVPENQLSISEGAS"
     gene            complement(164..646)
                     /locus_tag="ACHX5T_004424"
     CDS             complement(164..646)
                     /locus_tag="ACHX5T_004424"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8469301.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(637..939)
                     /locus_tag="ACHX5T_004425"
     CDS             complement(637..939)
                     /locus_tag="ACHX5T_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR8469302.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
BASE COUNT          328 a          273 c          233 g          294 t
ORIGIN      
        1 ggaagcgcct tcactgatcg aaagctgatt ttcaggaacc gttttgacgg cttcggcttt
       61 aaactccgta gagtaacgtt gggtttttct gctcattatt ggctccttct gatgccattc
      121 tatttcagga aggagtgtcc gttaaactca ggctacctca gactcagact ttataaaaca
      181 aggtattagg tgccacaact gactgaacaa aaccaaaacg ctcataaaat gctctggcgt
      241 catcatcaat tgcatgtacc ataacagcct taatgccaac ctgatcagcc agattgacaa
      301 cccggtgaat ggcatcactt agtagccatt taccgaaacc atgcccctgg gtacaaacat
      361 caacagccag acgccctaac agaacaacag ggacaggatt gggcatgtta tgccgcaaac
      421 tgcgaccaag ctcggcatga gtgacggaac cagtagctaa tgagtagtat cccacaatac
      481 gtaaagtatc ctccaagcaa ataacgaacg tgcgagaagc attgagcatc tggtttttca
      541 tagcccgacc acgtaaccag ttacttagca cctcatttcc acagtcaaat ggctgaagta
      601 tatgctcatc cgtcaacaat gctggcgcgg tgacatttat ttccattcag gctttaccgc
      661 catcagacgt tcacgccctt ctgcattctg cacaggagct tccagttgcg taataaacga
      721 atcataacgt tcgttatcca gcacaaataa acgttgatcc aggataatgt ttttagcttc
      781 ctggtacgcc agttccatga tgaaatcggt acggttctta ttgaccaagc tcgcagcata
      841 atcaataacg gctcgttcct catcagttgc ccggatgtta atctgatgtt ctcgcggtgt
      901 tttacggacg cttgcagctg tagacataaa aacctccatt ggtaacaatt tgctatgcaa
      961 ttaatgttat cgcaaatggt aacagaacgc tatacagaat cacttacggg aaaagcgatc
     1021 ccctgataaa aacaatggca gagacctgaa cattgtgacg atgatctgtg tcggcgcaca
     1081 aatcatgtct gattacggcc gtttaatgtg attttgataa tgaaatgc
//
LOCUS       DAZSIO010000415         1109 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZSIO010000415 DAZSIO010000000
VERSION     DAZSIO010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.422"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..1019
                     /locus_tag="ACHX5T_004426"
                     /pseudo
     CDS             1..1019
                     /locus_tag="ACHX5T_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            992..>1109
                     /locus_tag="ACHX5T_004427"
     CDS             992..>1109
                     /locus_tag="ACHX5T_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469303.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQV"
BASE COUNT          312 a          271 c          258 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggtta
//
LOCUS       DAZSIO010000416         1085 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZSIO010000416 DAZSIO010000000
VERSION     DAZSIO010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.423"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..1038
                     /locus_tag="ACHX5T_004428"
     CDS             <1..1038
                     /locus_tag="ACHX5T_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR8469304.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
BASE COUNT          297 a          264 c          288 g          236 t
ORIGIN      
        1 ttctttccga accttgttga tcaccttctg gttaagcatc acacggctga tggcgttgaa
       61 gatggcattt caatctgcta cttcaagcca tactcgcaag gccgtgctcg ctggcagggt
      121 gacacaatcc acggcgtgtg gtttgacgaa gagccaccat acagcattta tggcgaaggt
      181 cttacccgta ccaacaaata cgggcaattc tcaattctga cgtttacccc gctgatgggg
      241 atgtctgacg ttgttaccaa gttcctgaag aaccccagca agtcgcagaa agtggtcaac
      301 atgaccatct atgacgctga gcactatacc gacgagcaga aagagcaaat catcgcatca
      361 tatcccgagc atgagagaga ggcgcgtgct cgcggtattc ctacgatggg tagtggtcga
      421 atcttccaga taccggaaga gacgattaag tgtcagccgt tcgagtgtcc tgatcacttc
      481 tacgtaattg gcgggatgga tttcggatgg gatcacccac aggcgcaagt tcagctttgg
      541 tgggataagg aggcagacac aatctacgtt tcacgcgtgt ggaaggcgaa agaaaaaaca
      601 gccgttcagg cgtggggagc cgttaaatca tgggcgcata aagtgccaac cgcatggcct
      661 catgatggaa accagcacga gaagggcggc ggtgagcagc tcaaagggca gtatgcagac
      721 gctggattta tgatgttgca ggagcatgcg acatggcctg atggcggtaa cgctgttgag
      781 ccaggcatca ctgaattgcg cgacatgatg ctcgatggtc gcttcaaagt attcaatacc
      841 tgtgagccat tctttgagga gttccgcctc tatcaccgtg atgaaaacgg gaaaatcgtc
      901 aagcttaacg acgacgtgct atccgccgtt cgctatgcat acatgatgcg ccgcttcgca
      961 aaaatgatgc gcgacatcaa aaaaccaaaa gagaaaaaga taccagcccc aatcaggccc
     1021 atcgcacgga gaacttaaat ggccgacgaa aacagactca attccattct gtgtaagttt
     1081 gacgc
//
LOCUS       DAZSIO010000417         1072 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZSIO010000417 DAZSIO010000000
VERSION     DAZSIO010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.424"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..160)
                     /gene="ospE1"
                     /locus_tag="ACHX5T_004429"
     CDS             complement(<1..160)
                     /gene="ospE1"
                     /locus_tag="ACHX5T_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HFR8469305.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAA"
     gene            complement(342..743)
                     /locus_tag="ACHX5T_004430"
                     /pseudo
     CDS             complement(342..743)
                     /locus_tag="ACHX5T_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          286 a          222 c          225 g          339 t
ORIGIN      
        1 tggcagcagg tatttgtaat tcgtatatag ctgctttttt attgaaaaca gtatatccat
       61 ctgtagtaac agtagaagat aataatacag gatttgatac ttcaaggatg atattttctt
      121 gtttttgtgg aaggcaaggg aatatagttt gtgtaagcat attgcttcct tatgttatat
      181 tttagtaaag aatttatttt ttacattata gtcggctaaa tgaagtttac tgtaaaaaaa
      241 cgatactttt ttcgtaagcg atgagatgtg gagccagaaa aatggctgcg ttacgtcatt
      301 gaactgtcct tgacccacga tattcagaca gtcgttgtat cttaagttga cattacccgc
      361 tgacgtcgat attcccttgg tgagagataa cccaacgcac tatgcggatg gttttcattg
      421 taatgctcga tcgccactgc aaggttatgc aatgccgttc ttggtttcgg catgaacgcg
      481 atgcagtctt ccttcatcgt tttcatgaac cgttctgcta ttccattact ctgagggcta
      541 ctgactgccg tcgtgcaggg ctccagattc aactctctgg cgaactgccg tgtttcatga
      601 gccgtataag ccgaaccgtt atcggccact ggattggccg ttccggtcct ttatcgccat
      661 atcgctcctg acatgacatc ctgcaccgtt gctttgtcat aatctcccgt gcctgctgcc
      721 cagtctatgg cttcacgatc gcaatatgga acgtaacgct gtcatcggga gaatcgtcaa
      781 tgcggcattc ggttaacgct taccaatatc aacaccaacc ggatttgtgg agcctccagt
      841 ctgaaggggc tttatgcgtt ccgtatagga tattatgatg ctggagtttt gcgaagctcc
      901 actcctttcc gaacatttac acagatattg cctaagaaaa gtgaaaccat aatatggtta
      961 tagtcccttt cagtgcaaat acttagcttg ttgcacagag aaatagaagc tgcataagct
     1021 ctttcttcaa aaaatgtaaa taaagttaaa tataggaaaa attacttaat ct
//
LOCUS       DAZSIO010000418         1070 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZSIO010000418 DAZSIO010000000
VERSION     DAZSIO010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.425"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(344..1039)
                     /gene="aqpZ"
                     /locus_tag="ACHX5T_004431"
     CDS             complement(344..1039)
                     /gene="aqpZ"
                     /locus_tag="ACHX5T_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFR8469306.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT          301 a          322 c          251 g          196 t
ORIGIN      
        1 tttaatccta tctgagtgtt tagcattgtt gcgctccttt catgtgcaat gccatataaa
       61 gccactggcg gagggagcgc gcagggggca gccagtcgcc gccgccccct gcacccccgg
      121 gctctggcga acaaaatcgc cgctgcgcgg taccttcagc ttatcccctt cggcaaccgg
      181 gtcgggcgcg aggtaacgtc ctgtaagacg cgccctcagc ccacatccat gtgggctgcc
      241 ccggccttcg gggaacgctt cagcgatttt gacgccacca atacccgtgc ggtttgtaat
      301 gtcgaagata aaacaaccag ggcagataaa atgccaatgc taattaatca cgcttttcca
      361 gcagagtccg gtaaatcaga ccaccgataa tgccgccgac aattggcacc acccagaaga
      421 accacagttg ttctaatgcc cagccgccct ggaagatagc aaccgcggtg ctgcgcgccg
      481 ggttaacaga agtgttagtc accggaatac tgattaagtg aatcagggtt aaggccagac
      541 caatagcgat cggcgcaaaa cctgccggag cgaatttgtc ggttgcaccg tggatcacta
      601 acaggaagcc cgcactcaat accagttcaa ctaccagcgc ggaaagcatg gaataaccgc
      661 ctggtgaatg ctcgccataa ccgttagaag caaaaccgct ggctgccgcg tcaaaacccg
      721 ttttaccact ggcaattaaa tacagcagcg ccgctgcaac aataccgccg acaacctggg
      781 caattacgta gccaacgact tcttttgccg gaaaacgtcc gccagcccat aaaccaatag
      841 tgaccgccgg gttaaaatga ccaccagaaa tatgaccaac agcgaaggcc atcgtcagaa
      901 cggtcaaacc aaatgccagc gccacgccgg caaaaccaat gcctaattcc gggaagcctg
      961 cggccagtac agcactacca cagccaccaa aaacaagcca gaaagtacca aaacattcag
     1021 ctgctaattt tctgaacata tccacctcaa ttaaaaattg accctgtgaa
//
LOCUS       DAZSIO010000419         1069 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZSIO010000419 DAZSIO010000000
VERSION     DAZSIO010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.426"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(227..652)
                     /locus_tag="ACHX5T_004432"
     CDS             complement(227..652)
                     /locus_tag="ACHX5T_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFR8469307.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            complement(649..1032)
                     /locus_tag="ACHX5T_004433"
     CDS             complement(649..1032)
                     /locus_tag="ACHX5T_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFR8469308.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
BASE COUNT          253 a          308 c          265 g          243 t
ORIGIN      
        1 ctcgcgtatg gttgattgac accacagtgc gaattcggcg aatgcgagat cacggtacga
       61 ttccgcacca cactaccaat caataccccg ggttttattc aggtaagcag gactgcggat
      121 atccggtgtc gcgcctttgt gtcacgaacg gggtgggtgc gaaacaccag ataaaataca
      181 ggttggcaat acctctgaac gccctgagca ggtcgggcat ttcggattaa gtactcgcac
      241 ctccgcagtc ctcaaacaag tctggctggt agctgtaaac agacttcgta cataccgctc
      301 tgcaataaat ccccgtacca caggcctcac agaacttctt cccatgaaaa aactgctcac
      361 acatcacaca atgccactcc agcacgaccg gtaatggcga taaaaacatc gccatatcct
      421 caatgtaagg gtgggacttc tccggattca gcaccacaca gtccgccttc tgttgctcac
      481 tcagggcatg taaatcatgc tcaaaccacg ccccctgagc atctgtctgc agaatcaacc
      541 ggccatggca ggaaaggcag aaacactgcc ggatatttct gctgagcgca gagtcggcac
      601 tgataatgtg ctcacccggc gtgatcccca gccccgtttt tataccgttc attctgctcc
      661 cccctcctca ctgaagcgcc cggtctggca gtgtgtgcag tactgctccc cataataatc
      721 gtggtgacat tgcctgcagt gccagctggc tttacgtact acgggtaagg tatccgggac
      781 gaattgctgc agacgtttaa tcaaccgtat ttctctgcgt tccggcctga cataagggca
      841 ctcatgaccg tgctccgtca gcccttcgtc agcgtgttca aaccagggcc gttcagtgtc
      901 gtattgcggg tggtatctga gtgcactgcc gcaaaggtgg caggtgtagc tatcgtaagg
      961 tgcagtctgt gcggtacggg caccggtcag acgtccgttg ccatcaagtg cgagaaaaga
     1021 ttttgcgtac atagtatatg ttcctcagcc gccagacgac acgcaggca
//
LOCUS       DAZSIO010000420         1068 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZSIO010000420 DAZSIO010000000
VERSION     DAZSIO010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.427"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..560
                     /locus_tag="ACHX5T_004434"
     CDS             <1..560
                     /locus_tag="ACHX5T_004434"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HFR8469309.1"
                     /translation="TKGDAMIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDN
                     YQAIQNAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKS
                     GLTGSVLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMV
                     NEVETALFNARALALKIYSMSGWNL"
     gene            787..>1068
                     /locus_tag="ACHX5T_004435"
     CDS             787..>1068
                     /locus_tag="ACHX5T_004435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HFR8469310.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          314 a          202 c          257 g          295 t
ORIGIN      
        1 acaccaaagg cgatgccatg attggcgtca agcagccttt accaaaagca gttttaagaa
       61 ctcagcatga caaaaataaa gaagcaataa gtatcctgga ttttggtgtt attgatgatg
      121 gtgtgacaga taattaccag gcaatacaaa atgctataga tgccgttgct tcactaccct
      181 ccggcgggga gctgtttatc cctgcgagca accaagcggt ggggtatatt gttggatcca
      241 ctttgcttat tcctggcggt gttaacatca gaggagttgg taaggcatcg caactccgag
      301 caaaaagcgg acttacagga tctgtgttaa ggctgtctta tgattcagac actatcggcc
      361 gttatctgag aaatatacga gtaactggta ataacacctg caatggtatt gacacaaaca
      421 ttacagcaga agactctgtc atcagacagg tttatggctg ggtatttgat aatgtaatgg
      481 tgaatgaagt tgaaaccgca ttatttaatg caagggctct ggcactcaaa atttatagca
      541 tgtcaggctg gaacctgtag agtcggtctt cactttttag gccagtgcgt aagtgttagt
      601 gtcagctcct gccatttcag cagaggaaat tattctgctg atgaaagctt tggcatcagg
      661 attcagcctc aaacgtatgc gtggtcgtca gaggcagtta ggtcagaagc aataatttta
      721 gacagtgaga ccatgtgcat tggttttaaa aatgccgtct atgttcatga ttgccttgat
      781 ttgcatatgg aacaactgga tttagattat tgcggctcaa caggcgtggt tatagagaat
      841 gtaaacggag gattttcttt ctcaaactca tggatagcag cagatgccga tggcactgaa
      901 caatttacgg ggatatattt tagaacgccg gcctcaacgc agtcacataa aattgtcagt
      961 ggtgttcata tcaacactgc aaacaaaaac acggctgcaa acaatcaaag tatagcgata
     1021 gaacagtcgg cgatcttcgt ctttgtaagt ggttgtacgt taactggt
//
LOCUS       DAZSIO010000421         1040 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZSIO010000421 DAZSIO010000000
VERSION     DAZSIO010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.428"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            117..1040
                     /locus_tag="ACHX5T_004436"
                     /pseudo
     CDS             117..1040
                     /locus_tag="ACHX5T_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT          324 a          155 c          209 g          352 t
ORIGIN      
        1 ctttttagtt tggttttagg atattgcgca tcaaaatatt tcatttttcg ttagtaaacg
       61 atttgttaaa aacacaatta cttgcacatt agctaaatag tttatatgag gggcttatgt
      121 atcactcttt taatatcgct gtaattattc cttgttataa tgagcagaag gcaatagcaa
      181 aagtcataaa tgatttcaaa acgaatattc caactgcttc tatatatgta tttgataata
      241 attcgactga cagtacagca caagtggcag aggatgctgg agcaacagtt cactctgttc
      301 ctctgaaagg caaggggaat gtcgtaagac gtatgttctc agatgtagat gcggatattt
      361 atctaatggt tgatggagat gacacttatg atgcaagttc tgctccagaa atgattaacc
      421 atctcataaa aaatcaattg gatatggttg taggatgtcg tcaagagaat ggagatcaaa
      481 atacatacag aaaagggcat cgctgaggaa ataaattgtt gactggtact gttcagacta
      541 tctttaaagg gaattttact gatatgttgt cggggtatcg tgtattttcc agacgctatg
      601 ttaaatcttt tccatgtcta tcaaggggat ttgaaatcga aactgagctt acaattcatg
      661 ctttagaatt acgtatgaag tatggtgagg ttaatacaaa atatggcgag cgatctgaag
      721 ggagtgtgag caaactatca acttggagtg atgggttcaa gattctgaaa acaataatta
      781 aactttactc gcttgaacgg ccactatact tttttagtat tatcggcgtt ttacttgctg
      841 ctttgtctat aattttagga ttgcccatca ttgttgatta tattgataca ggtttggtga
      901 gaagatttcc gactgccttt ttaactgcct ccatcatgtt atcgtcaata atggcatttg
      961 tatgtggcat tattttgcat tcaaatacaa caactagacg tgaaatgaag gcattatttt
     1021 atctttctga aaaaaactat
//
LOCUS       DAZSIO010000422         1035 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZSIO010000422 DAZSIO010000000
VERSION     DAZSIO010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.429"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(63..808)
                     /gene="nfeF"
                     /locus_tag="ACHX5T_004437"
                     /gene_synonym="yqjH"
                     /pseudo
     CDS             complement(63..808)
                     /gene="nfeF"
                     /locus_tag="ACHX5T_004437"
                     /gene_synonym="yqjH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
BASE COUNT          251 a          265 c          267 g          252 t
ORIGIN      
        1 gggccaccag atatagcaaa ggctgacgag aaatcgtcag cctttttctt tttatatatc
       61 agttactttg cgtgccagta agccgctgca cgtacccgct gtgggtcata ctgttccgct
      121 tcaaagcggc ggcttaaatt cttaacgact ttaccttcgc cggttatcca gatgaagtaa
      181 tcatcggcag ggatttgcat ctgcgccaga cgcgcatcta ccgcctgctc atcatgtgcc
      241 agccattcga tattaaaacc atcaaggtgc gcgagataat cctgacaggc gttatcccgc
      301 acgctaacca gcgcactaac ttgcggttta acggcaagtt tgctcaacgt ttccaggcgg
      361 cggcgcagtg cgggcattcc ggattcatcg cagacataca gctgatacgc gtaatcttcc
      421 ggcaccacca gcgaaccgcg cggacctgcc accgtaagtt tatcgcccag ccgctggcga
      481 ccccaccgtc gtgaataaag aaatcgatcg ccagttcatg gcgtagttcg tcatacagtg
      541 gcgtatagtc acgcgacggt gggcgtggtc cttccggcca gacgatgctc tcttccgtta
      601 ccgttggcgg cacaaagtga gcgtcaggtt gaggaaagaa gagtttgctg tgatcgtcaa
      661 agccgcgcga tgtaaaaccg tccaacgcct cgccgccaag gacaatgcgc tgaaaaccgg
      721 cgctgatgcg ctcaacgcgt agcacagtca gttcacggaa gcgcagatca ttgcgaacgc
      781 gctgcgggta gcggggggtg ttattcattg ttatcgcctt cgtgatggta atcagatata
      841 tctaaataaa attcgcaaat gataatgatt gttaatcatg ataaatgcaa gcgatttgta
      901 gaactgatat gtctgaagta tgtgaaggca ttcagtgcca tagcaatgct gccggatgcg
      961 gcgtgaacgt cttatccggc ctagatgtga ggtgcttcca cgtttaaaaa tcatttttgt
     1021 acttgcagta atgct
//
LOCUS       DAZSIO010000423         1019 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZSIO010000423 DAZSIO010000000
VERSION     DAZSIO010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.430"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(50..190)
                     /gene="yqcG"
                     /locus_tag="ACHX5T_004438"
     CDS             complement(50..190)
                     /gene="yqcG"
                     /locus_tag="ACHX5T_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope stress response protein YqcG"
                     /protein_id="HFR8469311.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            329..1000
                     /gene="queE"
                     /locus_tag="ACHX5T_004439"
     CDS             329..1000
                     /gene="queE"
                     /locus_tag="ACHX5T_004439"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR8469312.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          295 a          205 c          237 g          282 t
ORIGIN      
        1 acatacgaaa gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga
       61 taaataaatt gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag
      121 aaaaaataaa tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt
      181 cttctgacat aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta
      241 aatctgcatg taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt
      301 aacaattatc cacttcacag agaatgctat gcagtacccg attaacgaga tgttccagac
      361 cctgcaaggt gagggttact ttaccggcgt tcccgccatt tttattcgtt tacagggatg
      421 cccggttggc tgtgcctggt gcgacaccaa acacacctgg gaaaagcttg aggatcggga
      481 agtctccctt ttcagcattc tggcgaagac caaagagagt gataagtggg gggcggcgag
      541 cagtgaagac ttgctggctg tcattggtcg tcagggatat accgcgcggc atgtggtgat
      601 tacgggtggt gagccttgca ttcatgattt gctgccactg actgacctgc tcgaaaagaa
      661 cggttttagc tgccagatcg aaaccagtgg aactcatgaa gtacgctgca caccgaatac
      721 ctgggttacc gtatcgccaa agctgaacat gcgcggcggc tatgaagtgt tgtcacaggc
      781 actggagcga gccaacgaaa tcaagcatcc agtggggcgc gtacgcgata ttgaagcact
      841 ggatgaacta ctggcaacgc tgaccgatga taaaccgcga gtcattgcac tgcagccgat
      901 tagccaaaag gatgatgcca cacgattgtg cattgaaacc tgtattgcgc gtaattggcg
      961 tttgtcgatg caaacacata aatatttaaa tattgcctga ttacacattt ataagcgtt
//
LOCUS       DAZSIO010000424          998 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZSIO010000424 DAZSIO010000000
VERSION     DAZSIO010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.431"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
BASE COUNT          322 a          194 c          172 g          310 t
ORIGIN      
        1 acgaatctaa taataaccct catcattaat gcaactaatc ttatctatat atcatgtgat
       61 atgttctgta acaagtaaaa tcaacataaa aaattcagca aaatttaata tttgttaaac
      121 tagccgtctc aaatatgaca ttattagtta actgcatttg ccacaaaatg gctatttcat
      181 ggaacgcagt aatttcttag tggctattat catgctaatt cttagatccc tagcgattat
      241 tcctgcctgg ttcgtcagct taggccatga gattgttaca ttccttttta tcctggagat
      301 atgggggaac gcaggcgacg ctttttcatc tacatccgtt ggtaaatata gctttggtca
      361 ctacgactta agccttcctc aatcacagct attcgccggg cgatacggca aacaaactaa
      421 gtgaaacatc atcctgaaaa caccaacatc aacaagcctc tccagatcga catcaggagt
      481 gaccagttac aagccacaaa ccaagaaaca tatttcatga tgagaattat gctcagcctg
      541 atggcgagta aacccttatt ttcaccactc tcactgagtt agcttgcaaa gctttctttg
      601 tttagattct aaataaatta tttataaaac gtatagttaa aagaaaatct ttagtttttg
      661 aagaccaccg caagtgttcg tctggcttca catggcatct tcttcttaga aaaagatcga
      721 catattttgt gacgcgaatt gcaaatctgg ttttgttgta tggattgcgt gatttttgat
      781 ctggtataac aggtataaag atgcaccacg atagtcaatg agactgggca tctcgtaatc
      841 tatggcaaac atcacttcag ttctttcaca tcgggtgatg aaaatgcact tcagtctgaa
      901 aggaatatga aaatgagatc aacagacact ctattttatg actctgggta aaatgggttg
      961 agtaagtgat ataacttacg aatattcaaa tcaattaa
//
LOCUS       DAZSIO010000425          980 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZSIO010000425 DAZSIO010000000
VERSION     DAZSIO010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.432"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..258)
                     /locus_tag="ACHX5T_004440"
     CDS             complement(<1..258)
                     /locus_tag="ACHX5T_004440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469313.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKI"
     gene            complement(404..>980)
                     /locus_tag="ACHX5T_004441"
     CDS             complement(404..>980)
                     /locus_tag="ACHX5T_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469314.1"
                     /translation="YFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFST
                     LFVILFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQN
                     ADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINP
                     LKDHLGYAYIDLTLSLFCCSQYSYIYSLRKQ"
BASE COUNT          380 a          186 c          149 g          265 t
ORIGIN      
        1 tattttatta ttcctaagtg taaagacaga cagtatagca taaaaaacaa catcactgcc
       61 aactttatta attgcttgtt gcaaatctaa tgtggcagga cttataccat gtcgagcttg
      121 ttctgctgtt tgaacttgta gatcaatttc attccatagg tagtgaccaa atatatttcc
      181 tccagcagga ctaaaaggat caccagagat ccaaaaacta cggacatacc aataaatgcc
      241 accaagaatt gttgccaagc taaaaataag aaatgtctgt atgtttctct ttttcgctat
      301 caatataaca aatatgatgg ctccatatat ggcaccaaat agttttacgc cacaagcaat
      361 tccaataaaa aaaccacata aaaaaattaa ttcattttgt gttttattgt tttctgagcg
      421 aatatatata agaatactgg ctgcagcaaa acaaagatag cgttaaatca atatatgcat
      481 aaccaagatg atctttaagt ggattgatag taaatagtag gattgtagca ataatgcctg
      541 gataataagt tttcgtaatt tcccatgaac agccaacaag tccacaggct attacaaaaa
      601 aacagatatt tgctaaaaac tgagctaacc ttacatctcc taactgtaaa cctaaagcca
      661 ttaaaagatc tgcattttgc gggaacagag gaaaacgcaa atattgttca acaacaattg
      721 catggtgctc aactaatgaa cgagccaaag gtaagtgata catagtatca tcccaatcac
      781 cgggagctcg cagggacgat aacaatgtaa ccacaaaaat caaaacaaga aataaaataa
      841 caaacagcgt actgaactca atattattta ttgccagcca tctaaattgc tgacgtttaa
      901 taatgcttaa aacccaacac accgtaaaaa ttaaaaagat agcaatctta ttaaattgcc
      961 cgaccatgcc cagaaagtaa
//
LOCUS       DAZSIO010000426          958 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZSIO010000426 DAZSIO010000000
VERSION     DAZSIO010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.433"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..>958)
                     /gene="mxiD"
                     /locus_tag="ACHX5T_004442"
     CDS             complement(<1..>958)
                     /gene="mxiD"
                     /locus_tag="ACHX5T_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HFR8469315.1"
                     /translation="DIVIPGVATVVERLLNNGKALSNRQAQNDPMPPFNITQKVSEDS
                     NDFSFSSVTNSSILEDVSLIAYPETNSILVKGNDQQIQIIRDIITQLDIAKRHIELSL
                     WIIDIDKSELNNLGVNWQGTASFGDSFGASFNMSSSASISTLDGNKFIASVMALNQKK
                     KANVVSRPVILTQENIPAIFDNNRTFYVSLVGERNSSLEHVTYGTLINVIPRFSSRGQ
                     IEMSLTIEDGTGNSQSNYNYNNENTSVLPEVGRTKISTIARVPQGKSLLIGGYTHETN
                     SNEIISIPFLSSIPVIGNVFKYKTSNISNIVRVFLIQPREIK"
BASE COUNT          300 a          187 c          150 g          321 t
ORIGIN      
        1 tcctttatct cacggggttg aatcagaaaa acccttacaa tattactgat attacttgtt
       61 ttatacttga acacattacc gattacagga atagaggaga gaaaaggaat agatattatt
      121 tcgttagagt tcgtttcatg cgtataacca cctatcaaca aacttttgcc ttgtggaact
      181 ctcgctattg tactgatttt ggtccttcca acttctggaa ggacagatgt attttcatta
      241 ttataattgt aatttgactg actatttcct gttccatcct ctatcgttaa agacatctca
      301 atttgtcccc gagatgaaaa gcgaggtata acatttatca atgttccata cgtaacatgc
      361 tccaacgaag aattgcgctc accaactaat gacacataaa aagtcctgtt attatcaaat
      421 atagccggaa tattctcttg tgtcaaaatc accggccttg aaactacatt tgctttcttt
      481 ttctgattta atgccataac tgaagcaata aacttattgc cgtccaaagt gcttatcgat
      541 gctgatgagc tcatattaaa tgacgcacca aacgaatcac caaatgaagc cgttccttgc
      601 caattaacac caaggttatt taattcagat ttatcaatat cgattatcca taatgataat
      661 tctatatgtc gtttggctat atctaactga gtgataatat cgcgaataat ctgtatttgc
      721 tgatcattac ctttgactaa tatagaattg gtttctggat atgcaataag tgatacatcc
      781 tctaatatag aactattggt aactgaacta aatgaaaagt cattactatc ctcagatact
      841 ttctgtgtaa tattaaaggg tggcatcgga tcattttggg cttgtctgtt actcagagcc
      901 ttaccattat ttaataaacg ttctacaaca gtagccaccc ctgggatgac aatatcct
//
LOCUS       DAZSIO010000427          940 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZSIO010000427 DAZSIO010000000
VERSION     DAZSIO010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.434"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            310..828
                     /locus_tag="ACHX5T_004443"
     CDS             310..828
                     /locus_tag="ACHX5T_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFR8469316.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTV
                     SGGTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVI
                     PYQGKDWVQLTE"
     gene            835..>940
                     /locus_tag="ACHX5T_004444"
     CDS             835..>940
                     /locus_tag="ACHX5T_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFR8469317.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQK"
BASE COUNT          283 a          194 c          294 g          169 t
ORIGIN      
        1 cagacaggcc gagaattgat gcctggcaga cgctggtgaa tcaccattac gggctgcatg
       61 accccaacgc cccggagaac cgccggatac tgactctccc tgataacctg ccggaaacga
      121 aacaggcgct tgcagagagc gtcacacgcg ggatagacgc tctttatcat gtcggggaga
      181 taaaaggtcg tcaggatgtg attcaggcgc tcacagaggc agggcttgaa gtggtcaggg
      241 tgatgcgaag cagtatcagc attgcagacc cgaacggtgg gaagaatatc aggctgaaag
      301 gagcatttta tgagcaatct tttacagacg ggcgcggagt tcgagaaaaa gctgaaagag
      361 agagccgaat ctacagagac aatgctgaac gacgagttca gcaagctcga aaaatctgta
      421 agcaaggctg tgacatcaaa cgagacgaaa atcaaagacg ctatagccct gttcacagct
      481 tcgacagagg aatcactgaa aaaacaccgg gaaggggtga aagaggcgat gatgcagcac
      541 aggaaggacg tgttaaagct ggcagggaat acgggcatga tgttactggg gatggtcctt
      601 ttcctgttta ccgtgagtgg cgggacgctc tggtatcttg gcgggatgat acaggcgaac
      661 ctggaagaaa tcaggataca ggaagaaaca ttgcagaaac tgaacgcgaa gacatggggc
      721 gtggagtttg tgcaggacgg gagaaggaaa tttctggtta tccctcaggg gaaatcggca
      781 acggtgattc cctatcaggg gaaggactgg gtacaactga cggagtgaca cagagtgacg
      841 gagctggaaa gacatttgct gaacgcctta gagcaactgc aacaggacta tatgcagcgg
      901 ctgaacgaat gggagagcgc cttcgtggaa ttgcagaaaa
//
LOCUS       DAZSIO010000428          934 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZSIO010000428 DAZSIO010000000
VERSION     DAZSIO010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.435"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            112..>934
                     /locus_tag="ACHX5T_004445"
     CDS             112..>934
                     /locus_tag="ACHX5T_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8469318.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
BASE COUNT          310 a          234 c          136 g          254 t
ORIGIN      
        1 atccgcctcc tccagaagtt cctgtctact gctgaaggga accaataaca ttgcaaagca
       61 gataaaaaag aatccaacac ctctcaacat aaagaataca ccctgatatt tatgctcccg
      121 acaaataaca atcacagatt aatttcaaat tcgttctcca cttattcaat cgacactagc
      181 cgcgcatatg aaaattatct aacccattgg actgaatgga aaaataaccg catacaagaa
      241 gaacaacgag acatcgcttt tcagcgacta gtatcatgtc tacaaaacca agagacgaac
      301 ctggacttgt ctgaattagg cctgacaaca ttacctgaaa tccccccggg aattaaatca
      361 attaatataa gtaaaaataa tttaagctta atctccccat tgcctgcgtc ccttacacaa
      421 cttaatgtca gctataacag acttattgaa ctgcctgctt tgcctcaagg acttaaatta
      481 ttgaatgcgt cccacaatca actaatcaca ctacccacac tccccatatc tttgaaggag
      541 cttcatgtct caaataatca attatgttct cttcctgttt taccagaact actggaaaca
      601 ttagatgtat catgtaatgg gctggcagtt ttaccacctt taccattttc tttacaagag
      661 attagcgcaa tagggaatct tcttagtgaa ctcccccctc tacctcacaa cattcactcc
      721 atatgggcaa tcgacaatat gttaaccgat attccatacc tgccggaaaa tttaaggaac
      781 ggttattttg acataaatca gataagtcat atcccggaaa gcattcttaa tctgaggaat
      841 gaatgttcaa tagatattag tgataaccca ttgtcatccc atgctctgca atccctgcaa
      901 agattaacat cttcgccgga ctaccacggc ccgc
//
LOCUS       DAZSIO010000429          933 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZSIO010000429 DAZSIO010000000
VERSION     DAZSIO010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.436"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(55..243)
                     /locus_tag="ACHX5T_004446"
     CDS             complement(55..243)
                     /locus_tag="ACHX5T_004446"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469319.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            complement(540..778)
                     /locus_tag="ACHX5T_004447"
                     /pseudo
     CDS             complement(540..778)
                     /locus_tag="ACHX5T_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088287.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          245 a          181 c          217 g          290 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc acctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc
      421 ttttcatatt cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt
      481 aaatgtgaga catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat
      541 tcattctttc tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc
      601 atggtcagac aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac
      661 tgagtcttaa ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta
      721 atgattctgg caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg
      781 ttcgcgtcca ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac
      841 atcgcagata gagtacttac gataattttt tattttatac aacagatgcc cacgaaaaga
      901 attattttca atagacacct gcattacaca aag
//
LOCUS       DAZSIO010000430          923 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZSIO010000430 DAZSIO010000000
VERSION     DAZSIO010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.437"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..200
                     /locus_tag="ACHX5T_004448"
                     /pseudo
     CDS             1..200
                     /locus_tag="ACHX5T_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            300..>923
                     /locus_tag="ACHX5T_004449"
     CDS             300..>923
                     /locus_tag="ACHX5T_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFR8469320.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQD
                     NAQETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDLKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIK"
BASE COUNT          293 a          217 c          205 g          208 t
ORIGIN      
        1 attggctacg taattaaaac agaaaataaa gaaaccccat tcactgtagt tgatatcgac
       61 ggtctatcag gaaactttaa aactcttgtt gaaggtgcca caaaaatgtg cctagtttac
      121 atcggaaata atctgcccgc agaaaaaaag ccgaatttct ggcaaatctg attgcaatga
      181 aattaaaagg tgaaatctga aaaaaagaaa gcctgcacag tgtgcaggcc tgagtgaaga
      241 acctgggaca tttattcatc actcgcagta attttaatct gagttgaggt taaaaaacaa
      301 tgagcacaaa accactcttc ctgttacgga aagcgaaaaa atcatccggt gaacctgacg
      361 tcgtcctgtg ggcaagcgac gattttgaat cgaccagcac cactctggac tatctgctcg
      421 ttaagtcagg taaaaaactg agcaactatt ttaaagctgt tgccacaaat tttcctgtcg
      481 ttaatgacct tccccctgaa ggtgagatcg attttacctg gagtgaacgc tatcaactca
      541 gcaaagactc catgacatgg gaactaaaac cgggagcagc gccagacgac gttcaccacc
      601 aggataatgc tcaagaaacc aaagaactgg cgggaggcca ggaagaaaac gcgcaggcag
      661 acgcccacga ggattgccag gattgcgaag tctctgtagc cactttgcgg ttcacacagc
      721 gtcttctgca catttttacg tatgctgccg gggatctgaa atacctgcat catgccaccc
      781 gtgaacaacg caaacacatt actgctcttg agatggatca ggaaaacagc tatgtccaga
      841 atctgctgtt ggccatacgc ggcatggcag aaccgacaac tctggataat gccgccctgc
      901 tccgcctgac tgatgcaatt aag
//
LOCUS       DAZSIO010000431          923 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZSIO010000431 DAZSIO010000000
VERSION     DAZSIO010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.438"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..923
                     /gene="espL1"
                     /locus_tag="ACHX5T_004450"
                     /pseudo
     CDS             1..923
                     /gene="espL1"
                     /locus_tag="ACHX5T_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
BASE COUNT          299 a          186 c          169 g          269 t
ORIGIN      
        1 tgctcagacc tgggctgata ggtatgttcg caactgtgca attgctattc tgcccctatt
       61 acctgtggga gttctagggg ctattttcgc gggtattgca tcacgaatgc catttgcccc
      121 tatacatcca tcggcaatgt tgttaataat ggctacaggc cagactcaac agcttattac
      181 attattcaaa cagttgccca tactccctga aaaagaaatc attgaaataa taactgcgca
      241 gaatagcgtt ggtacacctg ctttattttt ggctatgatg aacggacata ctgacaacgt
      301 gaaaatattt atgcaagaaa ttcagtcact ggtagataat cacatcattc atgaagataa
      361 tctggttaaa ttactgcaaa ctaaaagtgc taacgaaaca cctggacttt atatctccat
      421 gttgtatgga ttcgatgaaa taatcgatat ctttctgaat acattagcca ctccaatagc
      481 actaagagct tttaaacaaa aaactggtga tgagtatttt agccatgaaa atacatgatg
      541 gtgagccagg attatacgcc gcaatggaaa ataatcaccc tttgtgtgtc acacggttcc
      601 tctctaaaat taatggcatc gcctttaaat acaagttgag caaagctaac atcatggatt
      661 tattaaaagg agctacagca cagggaaccc ctgcattata catcgccatg agcaagggta
      721 atgaagacgt cgtgttatct tatatatcga cgctgggtgc ttttgcaaaa aaacattctt
      781 ttagtcaaca tcagttattt acactattgg ccgctaaaaa tcatgacaac atgtcagctg
      841 ttcatatagc cattcatcat aagcattata aaactgtaga aacatattat gctgctataa
      901 atgtcatcag ccaaagcctg agt
//
LOCUS       DAZSIO010000432          918 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZSIO010000432 DAZSIO010000000
VERSION     DAZSIO010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.439"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..241
                     /locus_tag="ACHX5T_004451"
                     /pseudo
     CDS             1..241
                     /locus_tag="ACHX5T_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            258..776
                     /locus_tag="ACHX5T_004452"
                     /pseudo
     CDS             258..776
                     /locus_tag="ACHX5T_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
BASE COUNT          226 a          245 c          229 g          218 t
ORIGIN      
        1 ggtgtatatc cgcactcgcg aaacatatgg aacccggcgg ctccagacgg agctggcaga
       61 taacggaatt atcgtcggtc gtgacagact ggcaggtctt cgtaaggagc tgaggctgca
      121 ctgtaagcag aaacgcaagt tcagggcgac cacaaactct gaccacaatc tgccggttac
      181 gccgaatctg ctgaatcaga actttacgcc cacagcacct aatcaggtct gggtcgcaga
      241 ttcagttgtc caggccttcc gaaatcaacc gactgaaggc gctggtcgcg aaactgcagc
      301 gtatgcagtt cggtaaaagc tcagaaaaac ttcgcgcaaa aaccgaacgg tggatacagg
      361 aagcacagga gcgaatcagc gcacttcagg aggaaatggc ggaaacactg ggtgagcaat
      421 atgacccggt actgccatct tccctgcgcc agtcttcagc ccgtaaaccg ttaccagcct
      481 cacttccccg tgccccccgg gtcatccggc cggaagagga atgctgtccg gcctgtggtg
      541 gtgaactcag tccactggga tgtgatgtgt cagagcagct ggagcttatc agcagcgcct
      601 ttaaggttat cgaaaaacaa cgccctaaac tggcctgtcg ccggtgcgac catatcgtgc
      661 aggcaccggt gccttcaaaa cccattgcac gcagttatgc cggagcgggg cttctggccc
      721 atgttgttac cggaaagtat gcagaccatc tgccattata ccgccaaagt gatctactat
      781 tccacacagc tatttagctc tctttttgtt gagctcccat gattctttca tttattgtta
      841 ctaattattt tatgttatca tgatgttaga acacgctctg ctctgtgtct ctattttatc
      901 tatcatattc tgaggatt
//
LOCUS       DAZSIO010000433          870 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZSIO010000433 DAZSIO010000000
VERSION     DAZSIO010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.440"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>870
                     /locus_tag="ACHX5T_004453"
     CDS             <1..>870
                     /locus_tag="ACHX5T_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR8469321.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAM
                     KKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSE
                     NGLQDFTRSLTNLLNALGGSGKSLGESLGSLMTSMSHGVDSLTDISYRVRAFFDEVTL
                     AYRPLNDTQRKIV"
BASE COUNT          263 a          153 c          209 g          245 t
ORIGIN      
        1 tttaagttca gcagcagtta aaacagcagg tgtacttatc agctttcagg ctgtactatc
       61 cgcttatcag cgagtaatgg aagttggcct tcaacgtgca agtgctgaac gttcaattga
      121 tttcgtattt tctaaagatg caaatcaagt aaaagatttc acaaagtcat tagctcaaac
      181 aacgggttta gatatcgctg aactgcaatc acagtttgct ggtttcggtg cttcagctag
      241 agagtctcta ggcattcaag gttctgaaga actttttaga aatatgattg gctattctcg
      301 tctgatgggg cgaagtgaag aagagattaa gcgagcactg acagcattat cacagatggc
      361 tggtaagggt cagattatgg ctgaagagct taaggggcaa ctagctgaag ctgttcctgg
      421 tatggttcag gtattcgcta aagcaaccgg aaagactgaa caggaattat ttgatgctat
      481 gaagaagggg gctttaaaat ctgccgatac tcttcagaag gtcactcagg aactgaataa
      541 acaaatcacc gctaaaggtg gctggaaagc tatttcagaa agtacacagg cacaactagg
      601 gaacttgaaa aactcttgga ataccacgct tgatagtatc ttcagaggtt ctgaaaatgg
      661 tctgcaagat tttacaagaa gcttaaccaa tcttttaaac gctcttggtg gaagtggtaa
      721 gagtcttggt gaatctcttg gtagtctcat gacttctatg tcacatggtg tcgattcatt
      781 gaccgatatt agttacaggg taagggcgtt ctttgatgag gtgacattag cttaccgtcc
      841 actaaatgac actcagagga aaattgttga
//
LOCUS       DAZSIO010000434          859 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZSIO010000434 DAZSIO010000000
VERSION     DAZSIO010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.441"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            121..>859
                     /locus_tag="ACHX5T_004454"
     CDS             121..>859
                     /locus_tag="ACHX5T_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR8469322.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRL"
BASE COUNT          263 a          154 c          202 g          240 t
ORIGIN      
        1 ttacgatatg ggcaacataa atataatcta aaaaagagta aggcgttgta ggttggttcc
       61 gcgttcatta ccttgttagt gaacgcggat ttatcagttg tcgaaatggt ttttataata
      121 atgaaaaatt ttaatgtact aaccaaagtc gtattttgct tgtcgttttt atcaagcgca
      181 tttcccacct atgctgcgga tgatgtcaaa gtgactcaaa acacttcagt ttttaaactg
      241 aagcttagtg caacgcgatt aatctatgaa caagggtcgg atggcgcagc gataactgtg
      301 acaaatccgc aggattggcc gatgcttgtg cagagtaaag tctttgaaga agataaagta
      361 actgcagcgc cctttattgt cactccgcca ctgttcaggc ttaatccagg ccagcaaaat
      421 aaaatgtggg ttgtgcagac tgcgccagct ttacaagaca gggaaaaatt acactggcta
      481 tgcgccaccg gcattacgcc taaaaagagt gatgtttggg ccgagaaaga taaagaaaaa
      541 attgctgaaa atgtgaacct gaacatccag gttaacgtac atacctgcat aaaacttatt
      601 accaggccat ctggtattga aggcaagatg gttaatgcga gtgagaagtt atcgtggaaa
      661 aagcagggtg ataaaattgt cgcggtcaat cagtcgccgt tctacatcaa cctttcttct
      721 ttacaggttg gcggtagcaa gattgagaaa ctagattata ttgcgccgtt ttcgcaacat
      781 gagtttgtta tgccaaaaac tagcggccag gatattaaat ggtctgtgat tactgatgaa
      841 ggtggtgaga gtcgtttgt
//
LOCUS       DAZSIO010000435          852 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZSIO010000435 DAZSIO010000000
VERSION     DAZSIO010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.442"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..>852)
                     /locus_tag="ACHX5T_004455"
     CDS             complement(<1..>852)
                     /locus_tag="ACHX5T_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFR8469323.1"
                     /translation="ARTEATNDLYFSRVSQWDDWLSNYTTLQYRGQFDVVRPEVRKLV
                     AEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMRHNTAKIAVNVGVREQIESGVGAW
                     RLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDANSKQMDKSDAKHCTVINALSRNGW
                     KEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVYVAEYYEVEEEKEKVFIYRDPLTG
                     EPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRVYKSIITCTQILKDREKIAGEHIP
                     IVPVYGEW"
BASE COUNT          179 a          237 c          207 g          229 t
ORIGIN      
        1 ccattcgcca tacactggaa cgattggaat atgctctccg gctatcttct cgcggtcttt
       61 cagtatctgc gtacaggtga tgatcgactt atacacacgc cgacgcttca ccttgcgctc
      121 tgctacctta atgaatccac gattagccag gtcgtcgatg acgtctttga tatcctgctg
      181 gtaatagctg accggctcac ctgtcagcgg gtcgcggtag atgaagacct tctccttctc
      241 ctcttctacc tcgtaatact cagcgacgta gacgacatca ttcgacaccc acgggaatag
      301 ccatgtatcg ttaggattct ggaaagatgg cagggtatcc gggtcaatac cgtaatcctc
      361 tgcgaactct ttccagccat tgcgcgacaa ggcgttaatc accgtgcagt gcttagcgtc
      421 gctcttatcc atctgcttgc tgttggcgtc ccatatgacg tgtgagcagg cttcatggat
      481 tggcaggcgt cggattacct gattgttgct tgttggatcg ttgtcttcgt actgcgtgac
      541 cagacgccat gcaccaacgc ctgactctat ctgctcacga acgccaacgt taacggcaat
      601 ctttgccgtg ttatggcgca tatcagtacg atacatcccc atcaatacat cggctgcatc
      661 aggattagcg ccgtctttgg gtcggaagag aacgtcgaca gggttccggc gcatctctgc
      721 gaccagtttc ctgacctccg ggcgaacaac atcgaattgt ccgcgatatt gcagggtagt
      781 gtagtttgat agccagtcat cccattgcga cactcggcta aaatacaggt catttgtcgc
      841 ctcggttctg gc
//
LOCUS       DAZSIO010000436          847 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZSIO010000436 DAZSIO010000000
VERSION     DAZSIO010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.443"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..521)
                     /gene="ospD3"
                     /locus_tag="ACHX5T_004456"
     CDS             complement(<1..521)
                     /gene="ospD3"
                     /locus_tag="ACHX5T_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HFR8469324.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPN"
     gene            597..746
                     /locus_tag="ACHX5T_004457"
                     /pseudo
     CDS             597..746
                     /locus_tag="ACHX5T_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          253 a          161 c          151 g          282 t
ORIGIN      
        1 tggttgggat cataaagctg aatgacaaaa tcccccccat ttttagtttc tttaatcttc
       61 aagcctaatg ccatattgtg atttgatgta cttatttcta gtgctctgct tttttcggaa
      121 tgaaaaatca tttcattgaa aatataaaaa ataatatctc cccatgaacc gttttcaaca
      181 aaataaataa tagcaggcac attaatagcc ttttcttctt cactcactga aacataattt
      241 ttaatttcat ccggaaaagc aaagtgatga taatccacgt gaccactact tttgttaaac
      301 tgtgctatcc agtaggaagc taaatgtcga cagacaataa gtcttccaga gtgatcggta
      361 acttggcaat ttagatttaa taaaaaactc ctcttgtcag aaaaatatgg caattgtttt
      421 gagtgcaata gagactggat tgtctcaaca gagacgttgt ctttatttat catattttgc
      481 aaacatattt tccttgatgg gattaaattt actgatggca tatgtatata ttcctttcat
      541 tgatgggttg tgacgcagac ttagtgttgt tgtactcttt gccctttgga tgattgatgg
      601 caaagattga tgtggtttgt caccgttact ctgaaactca ggggggtatc cggaacggcc
      661 attccagttc aggggggctg ctctatcgct gtaagcagtg cctgaagacc ttccagttcg
      721 actaccgcta caacggggcg aaacccatca aactttccaa ctattatcac attgagccat
      781 actgacaata aacaatatat cgatattttg tgcaaatact gccttttgct aaacgatatt
      841 caatttt
//
LOCUS       DAZSIO010000437          835 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZSIO010000437 DAZSIO010000000
VERSION     DAZSIO010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.444"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..318
                     /locus_tag="ACHX5T_004458"
     CDS             <1..318
                     /locus_tag="ACHX5T_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HFR8469325.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
     gene            complement(365..706)
                     /gene="symE"
                     /locus_tag="ACHX5T_004459"
     CDS             complement(365..706)
                     /gene="symE"
                     /locus_tag="ACHX5T_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFR8469326.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
BASE COUNT          213 a          183 c          215 g          224 t
ORIGIN      
        1 ttattcgatc atcttcgcga tgaagttatg cgcctggacg cagggatcac gcaggaagta
       61 ttgaagctat atattgcgtt taaagcggaa acgaattttg ttgatgttgt gccgcaaaaa
      121 agccgactgc gattgtcgct taatatgcag tttcatgaac tggtcgatcc gaaaggtatt
      181 gccaaagatg tgacaaatgt tgggcgctgg ggtaatggcg atgtagaaat tggtttcagc
      241 gacctcgcac aacttcctta cattatggga ttaattcgtc aggcatttga aaaacagatg
      301 gagagcgcgt tggtataacg cggcgaataa tacccggtag cactggcgct accgggtcaa
      361 tgtatcaggc gactttctgt ttacctgtaa ttaccccaat aaattcctgt acctgccttt
      421 gtttacgcgc cgacagctta cacaccttgc gtagtgactg catcagttcg ctctcttcgg
      481 cggcggcggg ttgggcggtg aggacaatac agccttccat cactttgaca tctaccgccg
      541 tgccggtggc aaaaccggcg gcttccagcc actgaccttt cagggtgatg gcgggaatac
      601 ggctgtaatc cgggtaacga ctcgcataac tcacggttaa ttgacgatta tttgccgggg
      661 agacttctgc ttctaacggt tgtgcaatag aatgcgtgtc agtcatgatt actattctct
      721 ataaatagtg attgtgatta gcggtgcgag tgtgttggcg cacatccgca ccgtgctaaa
      781 tacctggata catcatcagt aaatacaggg aaagtccagc taaaaattaa tgaaa
//
LOCUS       DAZSIO010000438          831 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZSIO010000438 DAZSIO010000000
VERSION     DAZSIO010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.445"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..326
                     /locus_tag="ACHX5T_004460"
     CDS             <1..326
                     /locus_tag="ACHX5T_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR8469327.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="ACHX5T_004461"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="ACHX5T_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFR8469328.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          252 a          184 c          154 g          241 t
ORIGIN      
        1 ttagtgagac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca agttctatgt tcttcatcca tgttaccagt aacagccaga
      481 atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgctccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag acaaatgtaa
      781 gcagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga g
//
LOCUS       DAZSIO010000439          825 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZSIO010000439 DAZSIO010000000
VERSION     DAZSIO010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.446"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(151..246)
                     /locus_tag="ACHX5T_004462"
                     /pseudo
     CDS             complement(151..246)
                     /locus_tag="ACHX5T_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(282..587)
                     /gene="ccdB"
                     /locus_tag="ACHX5T_004463"
     CDS             complement(282..587)
                     /gene="ccdB"
                     /locus_tag="ACHX5T_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFR8469329.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(589..807)
                     /gene="ccdA"
                     /locus_tag="ACHX5T_004464"
     CDS             complement(589..807)
                     /gene="ccdA"
                     /locus_tag="ACHX5T_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFR8469330.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
BASE COUNT          208 a          214 c          179 g          224 t
ORIGIN      
        1 aaggataaac aaaattggct gtaaaaaaac gatactgttt cagttaaccg tgaatggcct
       61 gtttgcaccg tattgttctt gtttttcggt tagttaaaca tcgtacttca tatttgaacg
      121 gtaagcggct cgtcagaacc gtattgatat ttactgagag gtcagatcaa ctttccaagg
      181 caacagatcg cgtacccggt ttgccggcca gtcctggata tgctcaatga cgtaacgcag
      241 ccattttgtc aacgccacga tttttgaccc tagcctgaca tttatattcc ccagaacatc
      301 aggttaatgg cgtttttgat gtcattttcg cggtggctga gatcagccac ttcttccccg
      361 ataacgaaga tcggcacact ggccatatca gtggtcatca tgcgccagct ttcatccccg
      421 acatgcacca ccgggtaaag ctcacgggag actttatctg acagcaggcg ggcactggcc
      481 agggggatca ccatccgtcg tccgggcgtg tcaataatgt cactctgtac atccacaaac
      541 agacgataac gactctctct tttataggcg taaaccttaa actgcatttc accagtccct
      601 gttctcatcg gcaaaagagc cgttcatttc aataaaacgg gcgatctcag ccatcccctg
      661 ctgattttca gcctgccagc gttccgcacg cagacggcgg gcctcttttt gcatggccgt
      721 atttaccagg cctgagatat tcacgtttgc ggatttaagt aactgatagc tgtcactgtc
      781 tatagtgaca gtaatacgct gcttcatgat aagccctcaa aaaca
//
LOCUS       DAZSIO010000440          809 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZSIO010000440 DAZSIO010000000
VERSION     DAZSIO010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.447"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..746
                     /locus_tag="ACHX5T_004465"
     CDS             <1..746
                     /locus_tag="ACHX5T_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8469331.1"
                     /translation="VDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWN
                     NVTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIAT
                     LGSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKL
                     SFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPL
                     GLSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT          231 a          175 c          187 g          216 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtg
//
LOCUS       DAZSIO010000441          803 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZSIO010000441 DAZSIO010000000
VERSION     DAZSIO010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.448"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            46..803
                     /locus_tag="ACHX5T_004466"
                     /pseudo
     CDS             46..803
                     /locus_tag="ACHX5T_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
BASE COUNT          234 a          169 c          198 g          202 t
ORIGIN      
        1 tactgcaccc attttgttgg acgatgaaat ggaatagccc ctgatatgtc aaagccaaaa
       61 tacccttttg aaaagcgcct tgaagtcgtg aatcactact tcactactga tgatggttac
      121 aggatcatct cggcacgttt tggtgtcccc cgaacccagg tcaggacatg ggttgccctc
      181 tatgaaaaac atggagaaaa aggcttaatt cccaaaccta aaggtgttag tgctgatcca
      241 gagttgcgta ttaaggtcgt gaaagctgtg attgagcagc acatgtccct taatcaggct
      301 gctgctcact ttatgcttgc tggtagtggt tctgtagcca ggtggctgaa ggtttatgaa
      361 gagcgcggag aagctggttt acgcgcgctc aagattggca ccaaaagaaa cattgcaata
      421 tcagttgatc cagaaaaagc ggcatcagca ttggagctgt caaaagaccg acgcattgag
      481 gatcttgaaa ggcaagttcg atttcttgaa acgcggcttg tgtatctaaa aaagctgaaa
      541 gccttagctc atcccacgaa aaagtgaaag tactcaacga gctaaggcag ttttatcctc
      601 ttgatgagct tctcagggct gcggagatac cgcgcagtac gttttattac catctaaagg
      661 ctctcagcaa gcctgacaag tatgcggacg ttaaaaagcg tattggtgag atttatcatg
      721 agaatagagg ccgatacgga taccgtaggg taacgctgtc tcttcatcga gaagggaaac
      781 agattaacca taaagctgtt cac
//
LOCUS       DAZSIO010000442          794 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZSIO010000442 DAZSIO010000000
VERSION     DAZSIO010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.449"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="ACHX5T_004467"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="ACHX5T_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR8469332.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
BASE COUNT          229 a          127 c          197 g          241 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaat
//
LOCUS       DAZSIO010000443          787 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZSIO010000443 DAZSIO010000000
VERSION     DAZSIO010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.450"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            4..287
                     /locus_tag="ACHX5T_004468"
                     /pseudo
     CDS             4..287
                     /locus_tag="ACHX5T_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            369..506
                     /locus_tag="ACHX5T_004469"
                     /pseudo
     CDS             369..506
                     /locus_tag="ACHX5T_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          236 a          156 c          186 g          209 t
ORIGIN      
        1 cagttgacat ctaataagtt gcgggcttta gctaatgaac tagccaaaag tctcaaaaat
       61 cctgaagatc tcagtcagtt tgattggatg ctgaaaatga aaccgtactc aatgctaata
      121 tgactcatca tcttgggtgc gagaaaaatc agcttagatc tggcagcaat tcccgcaatg
      181 gttgcttaac gaagattatc actactggag atgaacctct ggaaatccgt actctgcgtg
      241 atcgtaatgg cactttcgaa ccgcaacagc tgaagaaaaa ccagccctag attactaaat
      301 ttgtcgggcg atggattaac tggttcaggc tgtctggtgc tgaaggcctg aagtcactgc
      361 tgtcagtgat gtggccggct cttcatgaga caataacccg taatcatcag tgccgttcaa
      421 tatggccact gttgaaaaaa gttcgtcact ttatggagac ggttagccca cttcccggag
      481 aaaaacatag tctggacaaa gtgtagcggt attaggctca catatttaga tgtagataag
      541 ccggagctgg cagttaaatc taggcagcat cacagaaatg gacaacgccc cttaaggact
      601 ggcgtatggc aatgagctgc tttattatca agtttggtga tcgccttgac ggtcacttct
      661 gagtgaaggc gcttgcacag aatggtaaac ggactcgatt attgaatctt agggataaga
      721 tatatctacg atatgtcaat atacgaaatc gaaagaattt attggtgaat atagccattc
      781 aatgtgg
//
LOCUS       DAZSIO010000444          782 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZSIO010000444 DAZSIO010000000
VERSION     DAZSIO010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.451"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..666)
                     /locus_tag="ACHX5T_004470"
     CDS             complement(<1..666)
                     /locus_tag="ACHX5T_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFR8469333.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALP"
     gene            complement(656..>782)
                     /locus_tag="ACHX5T_004471"
     CDS             complement(656..>782)
                     /locus_tag="ACHX5T_004471"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HFR8469334.1"
                     /translation="MRVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT          251 a          203 c          177 g          151 t
ORIGIN      
        1 tggcagtgct attttgattc gacgttgatc aagtttctga taattccaac gacggtcgac
       61 gggcaccatc cagtcaatca acatacctac cgccagccct gtgatgacat caataaaatg
      121 atgctgccat gtcgtcagcg tcgaaatggc gatgagtaaa aaccatccgc cacagacttt
      181 acgccacctc acagccagat gctgacgaaa gtgacgccaa agtagccagc agagaataat
      241 atgcagcgaa ggagactggt tataaggcag atcaaacagt tcaagttgcg aaaatagcca
      301 tcctgtcacc ccactcactt caggacggat aaaactaaac ttcagcgggt agagaaaaaa
      361 accgcagcag gccattaccg ttgccagaat aagccggtgg acaagtcggc gttgttcgaa
      421 tgtcgagcta caaacgaaca gcgaaaatcc atataaaaga tccagactcc agtaaggaac
      481 aatagtccag ggaagaaaag ggatcgccgt ttcccaaccg aatacctgac tggggatatc
      541 atggctgtta aggtcctgaa ccgcggtgaa ctgattaaga gatccatagg tgaagaagaa
      601 aaacggggcc agcaacaata accagccagc gccttgtagc aatacgttcc gacgttcagt
      661 tatcacgacg caccgccatc gaaaccgtaa aaatgcccca cacatcaatc cgttgagtgc
      721 atttatcaaa tccggcatta cgcacgagcg aatccatctc cccttgcgaa cgcacgcgca
      781 tt
//
LOCUS       DAZSIO010000445          767 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZSIO010000445 DAZSIO010000000
VERSION     DAZSIO010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.452"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>767
                     /locus_tag="ACHX5T_004472"
     CDS             <1..>767
                     /locus_tag="ACHX5T_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469335.1"
                     /translation="ELPANYKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGD
                     PGFIFDKISHKLRHTVTEFDESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSL
                     GYYDQDDYHIQRGIRINGDSLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNE
                     LNGERLREPNEVFKRLGRAIRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYK
                     DFKNLEDKVEKRLQNRQTKYQNEINQLSALGVNFDDI"
BASE COUNT          292 a          127 c          146 g          202 t
ORIGIN      
        1 gaattacctg cgaactataa agacaaagaa ctcgtccgta ctatcattaa tgacaacata
       61 gttgagaaga ctcatgatat caataacaag gaaatgatct tcagcgcatt aaaagaaata
      121 tatgatggag atcctggttt tatcttcgat aagatatcac acaaactcag acatacggta
      181 acggaatttg atgagagcgg gaaaagcgaa ccaactgact tatttacctg gtacggtaaa
      241 gataaaaaag gcgactctct cgctattgta attaaaaata aaaacggaaa tgattactta
      301 tctctcggtt actacgatca ggacgactac cacattcaaa gaggaattcg tattaatggt
      361 gatagtctca cccaatattg tagtgaaaac gccaggagtg cttcagcgtg gtttgaaagc
      421 agtaaagcta tcatggcaga atcatttgca actggttccg atcatcaggt tgtaaacgag
      481 ctcaacgggg aaagactgag agaacctaac gaagttttta aacgtttagg tcgagcaata
      541 agatataatt ttcaagtgga cgatgcaaaa tttagacgcg ataatgtaaa agaaataatt
      601 tctactttat tcgctaacaa agttgacgtt gaccatcctg aaaataaata caaagacttt
      661 aagaatctcg aagataaagt tgaaaaaaga cttcaaaatc gccagacgaa atatcaaaat
      721 gaaattaatc aactatctgc actaggtgtt aattttgatg atattta
//
LOCUS       DAZSIO010000446          767 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZSIO010000446 DAZSIO010000000
VERSION     DAZSIO010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.453"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            79..>767
                     /locus_tag="ACHX5T_004473"
     CDS             79..>767
                     /locus_tag="ACHX5T_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR8469336.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQ"
BASE COUNT          257 a          129 c          168 g          213 t
ORIGIN      
        1 ttatgatata atatgccgaa ttaacacatg ttaacgattt accagtaatg taaataaatt
       61 ttcgaggaga tcattccagt gggacgtaaa tgggccaata ttgttgctaa aaaaacggct
      121 aaagacggtg caacgtctaa aatttatgca aaattcggtg tagaaatcta tgctgctgcc
      181 aaacaaggtg aacccgatcc agaattaaac acatctttaa aattcgttat tgaacgtgca
      241 aagcaggcac aagttccaaa gcacgttatt gataaagcaa ttgataaagc caaaggcggc
      301 ggagatgaaa cgttcgtaca gggacgttat gaaggctttg gtcctaatgg ctcaatgatt
      361 attgctgaga cattgacttc aaatgttaac cgtacgattg ctaacgttcg cacaattttc
      421 aataaaaaag gcggcaatat cggagcggca ggttctgtca gctatatgtt tgacaatacg
      481 ggtgtgattg tatttaaagg aacagaccct gaccatattt ttgaaatttt gcttgaagct
      541 gaagttgatg ttcgtgatgt aactgaagaa gaaggtaata ttgttattta tactgaacct
      601 gctgaccttc ataaaggaat cgcggcgcta aaagcagctg gaatcactga gttctcaaca
      661 acagaattag aaatgattgc tcaatctgaa gttgagcttt ccccagaaga tttagaaatc
      721 tttgaagggc ttgttgatgc ccttgaagat gacgacgatg tacaaaa
//
LOCUS       DAZSIO010000447          760 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZSIO010000447 DAZSIO010000000
VERSION     DAZSIO010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.454"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>760
                     /locus_tag="ACHX5T_004474"
     CDS             <1..>760
                     /locus_tag="ACHX5T_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR8469337.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          211 a          155 c          185 g          209 t
ORIGIN      
        1 gagcgcattc tgaccgaccg tgaaaatggt gaaaaaggcg ataattctca agatacctct
       61 tatgcccgtg taggcgtgaa aggtgaaact cagatcaacc ctgaaatgac tggttacggt
      121 cagtttgaac tagacctgga agcatcaaac agacacaacc ctgaccaaac tcgtttggca
      181 tatgctggtt tgagttacaa agattttggt tcattcgact acagtcgtaa tgtcggtgtc
      241 gcgtatgatg cagaagcatt tactgatatg tttgttgagt ggggcggtga ctcatgggct
      301 ggtactgacc tgttcatgac caaccgtacc aacggtgttg caacctatcg taacacagat
      361 ttctttggca tggttgaagg tctaaatttt gctctgcaat atcagggtaa aaatgaaggt
      421 acaggcaact acaaagcaaa tggcgatggc catggtttat ctgcaactta cactattgac
      481 ggtttctcat ttgctggtgc ttatgcaaac tctgaccgta ctgattggca gtctggtgat
      541 ggtaaaggcg aaagagccga agtttgggcg ttgtcaacaa aatatgacgc taacaatgtt
      601 tatgctgctg tgatgtacgg tgaatctcac aacatgaatt ccgacgacgg tgatgttgtg
      661 aacaaaacac aaaacttcga agctgttctg caatatcagt tcgactttgg tctgcgtcca
      721 tccatcggtt actcctattc taaagcactg gacgtagcag
//
LOCUS       DAZSIO010000448          759 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZSIO010000448 DAZSIO010000000
VERSION     DAZSIO010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.455"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            101..>759
                     /locus_tag="ACHX5T_004475"
     CDS             101..>759
                     /locus_tag="ACHX5T_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8469338.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LL"
BASE COUNT          278 a          153 c          107 g          221 t
ORIGIN      
        1 tagttataaa ctgaaatttc ttaatcttga ttataacaag ctagcatcgc tggatgtctc
       61 ccggcaagaa tctctgattg agttaagtgc ccatcacaat atgatcactg accttatatt
      121 acacaatcat cccaggatga aaacaatcac tttaaacgac aaccatattg cacatttaaa
      181 cgccaaaaac actacaaaac tggaatattt aaacttaagc aataacaatt tactgccaac
      241 caatgacatt gatcaactaa tatcatcaaa acatctttgg catgtattag ttaacggcat
      301 caacaatgat ccacttgcac aaatgcagta ctggactgca gtaagaaata taattgatga
      361 cactaatgaa gtgaccattg atttatcagg acttaattta accactcaac caccagggct
      421 gcaaaacttc acctctatca atcttgataa taaccaactc acacattttg atgcaaccaa
      481 ctacgataga ctcgtaaagc taagtctgaa tagtaatgct cttgagtcaa taaattttcc
      541 tcaaggcaga aatgtaagta ttacacatat atctatgaat aataatgctc tcagaaatat
      601 tgatatagat aggctttcat cagttactta ttttagtgcg gcacataatc aactagagtt
      661 tgtgcaatta gaatcttgcg aatggctgca atacctgaat ctcagccata atcaattaac
      721 tgatattgtt gcaggtaata aaaatgaact cttactgct
//
LOCUS       DAZSIO010000449          754 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZSIO010000449 DAZSIO010000000
VERSION     DAZSIO010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.456"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>754
                     /gene="spa40"
                     /locus_tag="ACHX5T_004476"
                     /gene_synonym="spaS"
     CDS             <1..>754
                     /gene="spa40"
                     /locus_tag="ACHX5T_004476"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HFR8469339.1"
                     /translation="SAVLPTLVQTKFVLATKAIKIDFSVLNPVKGLKKIFSIKTIKEF
                     FKSILLLIILALTTYFFWINDRKIIFSQVFSSVDGLYLIWGRLFKDIILFFLAFSILV
                     IILDFVIEFILYMKDMMMDKQEIKREYIEQEGHFETKSRRRELHIEILSEQTKSDIRN
                     SKLVVMNPTHIAIGIYFNPEIAPAPFISLIETNQCALAVRKYANEVGIPTVRDVKLAR
                     KLYKTHTKYSFVDFEHLDEVLRLIVWLEQVENT"
BASE COUNT          243 a          105 c          141 g          265 t
ORIGIN      
        1 tcggctgtgt tgccaacatt ggttcaaaca aagtttgttc ttgcgactaa agctatcaag
       61 attgattttt cagtattaaa ccctgttaaa gggttaaaaa aaatatttag tataaagaca
      121 ataaaagaat ttttcaaaag cattctgctt cttattattc tagcattaac aacctatttc
      181 ttttggatta atgaccgaaa aataattttt tctcaggtgt tttctagtgt tgatggctta
      241 tatcttattt gggggaggct gtttaaggat ataatattat ttttcttggc attttctatt
      301 cttgttatta ttcttgactt tgtgattgag ttcattttat acatgaaaga tatgatgatg
      361 gataaacagg agataaaaag agaatatata gagcaagagg gacactttga gacaaagtcg
      421 agaaggcgtg agttgcatat cgagattctt tcagagcaga ctaaatctga tatacgtaat
      481 tcaaaattag tggtaatgaa cccgactcat attgcaattg gtatttattt taatccagaa
      541 atagcgcctg caccttttat ttctctcatt gaaactaacc agtgtgcctt ggctgtcaga
      601 aaatatgcaa atgaagttgg tataccgact gtgcgtgatg tgaaattagc tagaaaacta
      661 tataaaacac atacaaaata tagttttgtt gattttgaac acttggatga agtcctacgt
      721 cttattgttt ggcttgagca ggttgaaaac actc
//
LOCUS       DAZSIO010000450          751 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZSIO010000450 DAZSIO010000000
VERSION     DAZSIO010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.457"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..728)
                     /locus_tag="ACHX5T_004477"
     CDS             complement(<1..728)
                     /locus_tag="ACHX5T_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009247 - glycolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HFR8469340.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKN"
BASE COUNT          238 a          142 c          105 g          266 t
ORIGIN      
        1 tgattttttc taagcaaaag aaatgcattt cttactcgat aataacgacg tactggcgag
       61 tgaattggta tgttaatccc acagattttt actgacttat ccccaatagc atgaaccatt
      121 tctatatcag gaatgatatg aactaatatg ccatagttta agcatctaag acaccattct
      181 gtatcgacat aatcaataaa aagttcctca tccatcattc caacaatctc aaaaacttct
      241 tttgaaacca tggttccgga tgagattgtt actgaacttt taaacggtgt ctgtccttca
      301 gtgacatgaa ttttctctcg aagaccattt tttttgatat tacaaatagg ataataatgt
      361 gacttatctc tatcaataaa aactgggcca gctatttttg cattttcatt atccatatag
      421 caggaaagtt ttttgataaa ctcattgcga atacggctat cttgatcaaa aaaaattatc
      481 ttctctgccc ctttttcaag agaatatcga atcccaatat tttgtgcttt tgctatacct
      541 tcattcctac caagacaaat taaatgaact ttttcaatgt ctgctaattt atctttcaag
      601 gtgaaattag tcctattatc gactactaca acgtaacaat tttgctcctt tagttcagtg
      661 atcagtgcat tcagattttt aagttcggga ttatatgtca caatgacagc gtaaatatta
      721 ctattcatta tccaagtgac tcagtaattg g
//
LOCUS       DAZSIO010000451          736 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZSIO010000451 DAZSIO010000000
VERSION     DAZSIO010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.458"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>736
                     /locus_tag="ACHX5T_004478"
     CDS             <1..>736
                     /locus_tag="ACHX5T_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8469341.1"
                     /translation="MHQINNIHSLTLFSLPVSLSPSCNEYYLKVWSEWEKNGTPGEQR
                     NIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNLLTYLPDFPPMLKVI
                     HAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHNNRLHILPLLPPELK
                     LLIVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVLMNNNLTTLPESVLR
                     LAQNAFVNVAGNPLSGHTMRTLQQITT"
BASE COUNT          249 a          157 c          127 g          203 t
ORIGIN      
        1 atgcatcaaa taaacaacat acactcgtta actctatttt ctttgcctgt gagtttgagt
       61 ccatcttgta atgaatatta tttaaaagtc tggagtgaat gggaaaagaa cggtacccca
      121 ggcgagcaac gcaatatcgc cttcaatagg ctaaaaatat gtttgcaaaa tcaaggggcg
      181 gaattaaatc tatctgagtt agatttaaaa acattacctg atttaccgcc tcagataaca
      241 acactggaaa taagaaaaaa cctattaaca tatctccctg atttcccacc aatgcttaag
      301 gtaatacatg ctcaatttaa tcaactggaa agcttacctg ccttacccga gacgttagaa
      361 gagcttaatg tgggtgataa caagataaaa gaattaccat atcttcctga aactctaact
      421 catttacgga tccataataa ccgattgcat attctgccac tattgccacc ggagctaaaa
      481 ttactgatag tttctggaaa cagattagac agcatccccc cctttccaga taagcttgaa
      541 gggctggctc tggctaataa ttttatagaa caactaccgg aattaccttt tagtatgaac
      601 agggctgtgc taatgaataa taatctgaca acacttccgg aaagtgtcct gagattagct
      661 cagaatgcct tcgtaaatgt tgcaggtaat ccattgtctg gccataccat gcgtacacta
      721 caacaaataa ccaccg
//
LOCUS       DAZSIO010000452          732 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZSIO010000452 DAZSIO010000000
VERSION     DAZSIO010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.459"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>732
                     /locus_tag="ACHX5T_004479"
     CDS             <1..>732
                     /locus_tag="ACHX5T_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469342.1"
                     /translation="SVVEKKLNSALPLEENTTFRSQAEWFNLQYELLVSGYPERAYNL
                     LSKLETESKNAKYYLDLTASRVAEEEAPETALTFLSKIGLNKPATLTSFESYINSWIK
                     SKQPEKAMQLLSLDENALNYYLPTVLVAYQIVPDKAISIYNSVYGDNVVIPYQQVTML
                     LTVAEGYQTKGDIKNAIHYADKALNMFDKAIAVDSSRESHYSDEYLKLMNIYAANGNK
                     EKAVVLSQRLHKAITESQSNYLSTL"
BASE COUNT          245 a          144 c          136 g          207 t
ORIGIN      
        1 attctgtcgt cgagaaaaaa ctaaactctg ctttacctct ggaagaaaat accaccttca
       61 gatcgcaggc tgaatggttt aatttgcaat atgaattatt agtatctggc taccctgaac
      121 gtgcttacaa tttactgagc aaacttgaga cagaatcgaa aaacgctaaa tattatctgg
      181 atttaactgc ctccagagtc gctgaagaag aagcaccaga gacagcctta acttttttat
      241 caaaaattgg gttaaataaa ccggctactc ttacatcctt tgagagctat atcaatagct
      301 ggataaaaag caaacaaccc gaaaaggcta tgcagcttct cagcctcgat gagaatgccc
      361 tgaattatta tttaccgact gtgttagtcg catatcagat cgttcctgat aaggcaataa
      421 gtatttataa ttctgtatat ggagataatg ttgttatacc ctatcagcaa gtcaccatgt
      481 tacttacagt tgcggagggt tatcaaacaa aaggcgatat aaaaaacgcc attcattatg
      541 ccgataaggc gctgaatatg tttgataaag ctattgctgt agatagcagt cgtgaatccc
      601 attatagtga tgagtatttg aaactcatga atatctacgc cgccaatggc aacaaagaaa
      661 aagcggtggt tttatctcaa cgtctacaca aagccattac cgaatctcaa tcgaattact
      721 taagtactct gg
//
LOCUS       DAZSIO010000453          724 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZSIO010000453 DAZSIO010000000
VERSION     DAZSIO010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.460"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>724
                     /gene="icsB"
                     /locus_tag="ACHX5T_004480"
     CDS             <1..>724
                     /gene="icsB"
                     /locus_tag="ACHX5T_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HFR8469343.1"
                     /translation="GVSADKVYIPLSGDNKTKDGKISHNLFGLDETNMSKFICKKKAD
                     AFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATPNDVYAYANKVRQR
                     IESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPKTFKPLSISLYKHP
                     TENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEEIIEKSIKIIDYYN
                     SLKSPDLGTKLYIHDLLQINKLL"
BASE COUNT          277 a          134 c          103 g          210 t
ORIGIN      
        1 ggggtcagtg ctgataaagt atacattcca ttatcaggtg ataataaaac aaaggatggt
       61 aaaatcagcc ataacctttt tggccttgat gaaacaaaca tgtcaaaatt tatatgcaaa
      121 aagaaagcag atgctttcag acaattggca aattataaat taatcagtaa atctgaaaac
      181 tgtgctggta tggcacttaa tgttcttaaa gcaggaaatt ctgaaatata ctttccactc
      241 cctgacgtta aattggttgc tacacctaac gatgtatacg cgtatgcaaa taaagttagg
      301 caacgcattg aaagtctcaa tcagtcttat aacgaaataa tgaagtatat tgaatctgat
      361 tttgatcttt ccagattgac tcaattacga cgcagctatc ttaaaagttt caataaaatt
      421 aaccttatcc acacaccaaa aacattcaag cctttatcaa tttcacttta caaacatcct
      481 actgaaaatg tatcttcaga agactttgat gcggtcatca acgcttgcca ctcatactta
      541 gtaaaatcag cacccagcaa tatgactcgt gtattaaacg aattaaaaac cgaagcaaca
      601 gataaaaaag aagaaatcat cgaaaaatca ataaaaatta ttgattatta caattctctt
      661 aaatcgccag atcttggaac caaactttac attcacgact tattacagat caataagctt
      721 ttat
//
LOCUS       DAZSIO010000454          704 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZSIO010000454 DAZSIO010000000
VERSION     DAZSIO010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.461"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..443)
                     /locus_tag="ACHX5T_004481"
     CDS             complement(<1..443)
                     /locus_tag="ACHX5T_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFR8469344.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPD"
BASE COUNT          214 a          172 c          131 g          187 t
ORIGIN      
        1 tcatctggat aatcggcatg agcaataaca tgcttgccgg tgcgactgtg tacttcaaga
       61 ataaagggca aatgctgaca tttttccagc gccgttttcg cttgtttctg ttgattatct
      121 gccagcgcaa taaaccagtc gccgccattc atcaaccaca aagacatctg ctggaatgcc
      181 agcgcatcca tcgccatctg ttcatgattg cctcttaccg cacgaaccca atgttgttcc
      241 agtaactgca gacaacgtaa actttgcggc ccacgatcga taacgtctcc cactgagata
      301 agtaaatctc gccacggatc aaaacgacaa tgccataatt tgcggcgcaa ctgctcaaga
      361 caaccgtgta tatcgccaga aagccagata tgtcgccatt gatgacccgc aattctctga
      421 taaacgggcg caggctgttt catcaatatt ttcctcccgc gctaaagatc acataatctt
      481 aacaagaatg ttaaaaaacg ctggactcag acagtagagt gtgtgttatg gttgactata
      541 aagtcagcga aggaaatgct tctggctttt aacagataaa aagagaccga acatgattcc
      601 tgttttcgtc aacaaacaat aaaacccttt aaaattaatg cgttgaataa atttacgttc
      661 atcttttaat ccctgacacg taccattgca tattaataca ttca
//
LOCUS       DAZSIO010000455          696 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZSIO010000455 DAZSIO010000000
VERSION     DAZSIO010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.462"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            32..493
                     /locus_tag="ACHX5T_004482"
     CDS             32..493
                     /locus_tag="ACHX5T_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR8469345.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..>696)
                     /locus_tag="ACHX5T_004483"
     CDS             complement(534..>696)
                     /locus_tag="ACHX5T_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR8469346.1"
                     /translation="ILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGL
                     AARQHVKKS"
BASE COUNT          204 a          152 c          160 g          180 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctggcgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaatg
//
LOCUS       DAZSIO010000456          696 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZSIO010000456 DAZSIO010000000
VERSION     DAZSIO010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.463"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..673
                     /locus_tag="ACHX5T_004484"
     rRNA            <1..673
                     /locus_tag="ACHX5T_004484"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          175 a          164 c          206 g          151 t
ORIGIN      
        1 ttaagtcgac cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg
       61 cccgcacaag cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt
      121 cttgacatcc acggaagttt tcagagatga gaatgtgcct tcgggaaccg tgagacaggt
      181 gctgcatggc tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc
      241 aacccttatc ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata
      301 aactggagga aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca
      361 cgtgctacaa tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa
      421 gtgcgtcgta gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta
      481 atcgtggatc agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac
      541 accatgggag tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac
      601 tttgtgattc atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg
      661 gatcacctcc ttaccttaaa gaagcgttct ttgtag
//
LOCUS       DAZSIO010000457          693 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZSIO010000457 DAZSIO010000000
VERSION     DAZSIO010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.464"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..525
                     /locus_tag="ACHX5T_004485"
                     /pseudo
     CDS             1..525
                     /locus_tag="ACHX5T_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            589..>693
                     /locus_tag="ACHX5T_004486"
     CDS             589..>693
                     /locus_tag="ACHX5T_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR8469347.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDIT"
BASE COUNT          193 a          143 c          166 g          191 t
ORIGIN      
        1 tgtgctgatc gtgcggatga ttactaccat gaccgtctgg cgctgactgt tgataattca
       61 acgatcgacg acaactacga acactacaca tataacggca cttacaataa tgccgctgat
      121 actcatgttg tagacgttta caacattggt acggctatta ctctggatca ggaagttgat
      181 ctgtccatca ctaataactc tcacgttgcg ggtattacgt tgactcaggg ttatgagtgg
      241 gaagatattg acgacaacac tgtcagcact ggcgtaaaca gcagcgaagt gtttaataac
      301 accatcactg ttaaagattc taccgtaacc tctggttcat ggactgatga aggtactact
      361 ggttggttcg gtaatacagg taatgccagc gattatagtg gtaaatctaa ctttgtcacc
      421 gtagatactg acggagatgg tgttgctgat agcactattg caagctggga tgatgttgca
      481 ttagctgtag ttgcacatcc gaatgttgat aatgctatgc agacctgaat cgccacgggt
      541 ttaacagaca cctcagagtc atttaagatg gcttaaagag aggtgcccat gagcggtaag
      601 cgttatcccg aagagtttaa aactgaagca gtcaaacagg ttgttgatcg cggttattct
      661 gttgccagcg ttgcaacacg tctcgatatc acc
//
LOCUS       DAZSIO010000458          685 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZSIO010000458 DAZSIO010000000
VERSION     DAZSIO010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.465"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..455)
                     /locus_tag="ACHX5T_004487"
     CDS             complement(<1..455)
                     /locus_tag="ACHX5T_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphate phosphatase SopB"
                     /protein_id="HFR8469348.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWG"
BASE COUNT          184 a          142 c           95 g          264 t
ORIGIN      
        1 ccccaattct tattatttaa ataatcagta aatatttgtt ttattttcgt ttgcaattcc
       61 tttttatttt taatgcctag ctgtgctctt atctcttcac ttattacttt attaagctca
      121 ttaatatgtc ttttattaac ttcaccaagt ttataggtat ctaatcgttc catcatggct
      181 ttaatttttg acgtatcacc acctgccaac tttattgcct cgcaaaaaac ttcagctctg
      241 tcacgcgcaa acaaaaggct ttttcctgtt aagcttgttt ggttctgtaa atataatcga
      301 ttaagcgctt cgatcccctt atttctttct ccatttttaa ctctttgata tgaaatcacg
      361 cttacacctt tgtgctttcc ggtttcttct gcgcctttat aggaatctcc gctttgaaat
      421 gaaacctgat gcaatcccaa attagttatg tgcatttaat tatcctcagt cataaatggg
      481 gcattgattg cagttttgta cctcgtttta caacaaaaaa gatacttatt caacagggtt
      541 ctctcttttt ctataacact ccgagcctga tacatcaggc actttatctt gtgggatttc
      601 atgatgaaac gagcactaca tatgctcacg aggtacatat tatgtacagc aaaagaaaat
      661 ttgattatgt taacaggctc aagtt
//
LOCUS       DAZSIO010000459          685 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZSIO010000459 DAZSIO010000000
VERSION     DAZSIO010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.466"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            52..685
                     /locus_tag="ACHX5T_004488"
                     /pseudo
     CDS             52..685
                     /locus_tag="ACHX5T_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          205 a          172 c          173 g          135 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctcccccat taccccggaa
      241 caaattgaaa tccgtgagct caggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttga
//
LOCUS       DAZSIO010000460          684 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZSIO010000460 DAZSIO010000000
VERSION     DAZSIO010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.467"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>684
                     /locus_tag="ACHX5T_004489"
     CDS             <1..>684
                     /locus_tag="ACHX5T_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR8469349.1"
                     /translation="LKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVAGTVNAA
                     NIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGAAASGAA
                     TAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVVETVGAT
                     PAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAYSHDLLS
                     ASLFEVTQW"
BASE COUNT          216 a          137 c          142 g          189 t
ORIGIN      
        1 ccttaaatat agtgctgcca ctggcggact cattgctgtt tctgaattag cgaaaagagt
       61 ttctggtaaa acaaaccgaa aacttgtagc aacaatgttg tctctggctg ttgcaggtac
      121 agtaaatgca gcaaatattg atatatcaaa tgtatgggcg agagactatc ttgatcttgc
      181 acaaaataaa ggtattttcc agcccggagc aacagacgta acaatcactt taaaaaacgg
      241 agataaattc tctttccata atctctcaat tccggatttt tctggtgcag cagcgagtgg
      301 cgcagctacc gcaataggag gttcttatag tgttactgtt gcacataaca aaaagaaccc
      361 tcaggccgca gaaactcagg tttacgctca gtcttcttac aaggttgttg acagaagaaa
      421 ttccaatgat tttgagattc agaggttaaa taaatttgtt gtggaaacag taggtgccac
      481 cccggcagag accaacccta caacatattc tgatgcatta gaacgctacg gtatagtcac
      541 ttctgacggt tcaaaaaaaa tcataggttt tcgtgctggc tctggaggaa catcatttat
      601 taatggtgaa tccaaaatct caacaaattc agcatatagc catgacctgt taagtgctag
      661 tctatttgag gtcacccaat ggga
//
LOCUS       DAZSIO010000461          679 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZSIO010000461 DAZSIO010000000
VERSION     DAZSIO010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.468"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..251)
                     /locus_tag="ACHX5T_004490"
     CDS             complement(<1..251)
                     /locus_tag="ACHX5T_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFR8469350.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
     gene            complement(272..>679)
                     /locus_tag="ACHX5T_004491"
     CDS             complement(272..>679)
                     /locus_tag="ACHX5T_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8469351.1"
                     /translation="HRALSEHKTLSVSWSHYRSTVHKCTFVFSPQVIKQRQFSGPHYK
                     VDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNV
                     YGAWMKDNNHEQIELLNRRLSESVPCMPHKKAG"
BASE COUNT          155 a          156 c          152 g          216 t
ORIGIN      
        1 ctttgcaggt aagtcagtaa tgtttcttgc tgatcgcgcc agacgttctg cacatcaaca
       61 ttttcattac tgagcagtat tttaactttg ctgacctgca cgccgttgtc gatccagcgt
      121 ttgatctcgc ggatccggtc aatatcggca tcgttgaaca gccgatgacc gccgtctgtc
      181 cgttgcggtt tcagcaatcc gtaacgcctc tgccacgcgc gtaacgtgac agggattaaa
      241 ggtaaaccaa ttgattttgc aagtttttat tttaccccgc tttcttatgg ggcatgcatg
      301 ggacactttc agatagtctt ctgttaagaa gttctatctg ttcgtgattg ttgtctttca
      361 tccatgctcc gtaaacattg aataccattt gtgcgtttgt gtggcccatc tggcttgcga
      421 taaaactagg attagctcct gcggcaagtg accagcatgc ataggtatgc ctggattgat
      481 acgattttct gtgtctcaga cctgcgcgtt ttaagatact tgtccatgac tccctgatgg
      541 agtcaacctt atagtgcggt ccggaaaact gccgctgttt tattacctga ggactaaaaa
      601 caaaagtgca tttatgcaca gtacttcggt aatgactcca acttactgat agtgttttat
      661 gttcagataa tgcccgatg
//
LOCUS       DAZSIO010000462          671 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZSIO010000462 DAZSIO010000000
VERSION     DAZSIO010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.469"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..515
                     /locus_tag="ACHX5T_004492"
     CDS             <1..515
                     /locus_tag="ACHX5T_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFR8469352.1"
                     /translation="GIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEINNLIIN
                     KYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGKISKQG
                     EAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGMQYAKR
                     HFTGIQTSCL"
     gene            457..>671
                     /gene="mxiN"
                     /locus_tag="ACHX5T_004493"
     CDS             457..>671
                     /gene="mxiN"
                     /locus_tag="ACHX5T_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HFR8469353.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIG"
BASE COUNT          232 a           82 c          145 g          212 t
ORIGIN      
        1 atggaattta taaaaaatat ctttcaataa tttttgatcc agcgttctat ataaatagaa
       61 atcggttgaa tttgccttct gaactgttag aaaatggcgt aatcagaagt gagattaata
      121 atctcataat taataaatat gatctaaatt gcgatattga acctttaagc ggggtaaccg
      181 ctatgtttgt tgccaactgg aatttacttc cagctgttgc ttattttatc ggtagtcaag
      241 agtctcggtt aattaatcat agtgagatgg ttattagtta ttatgggggc aaaatcagta
      301 agcaagggga ggccgctatt cgaagtggtt tttggcatct tattgcgtgg aaggaaaata
      361 tatctgttgg tatatatgaa agaataaact tattatttaa tccaattgct ttggagggaa
      421 attatacgcc tgtagaaaga aatttgtccc gattaaatga aggtatgcaa tatgcaaaaa
      481 ggcactttac cggtatccag acatcatgcc tatgatggtg ttgttataaa gagaattgaa
      541 aaggaattat gtaaaaccat aaaagataga gacactgaga gtaagaagaa agcaatttgt
      601 gttataaagg aggctacgaa aaaagctgaa tcactgcgta tagatgctgt atgtgatggt
      661 tatcaaattg g
//
LOCUS       DAZSIO010000463          670 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZSIO010000463 DAZSIO010000000
VERSION     DAZSIO010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.470"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..141
                     /locus_tag="ACHX5T_004494"
     CDS             <1..141
                     /locus_tag="ACHX5T_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8469354.1"
                     /translation="RNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRL
                     RN"
     gene            256..>670
                     /locus_tag="ACHX5T_004495"
     CDS             256..>670
                     /locus_tag="ACHX5T_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFR8469355.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPS"
BASE COUNT          170 a          179 c          174 g          147 t
ORIGIN      
        1 cgtaatgtcg ttgaacggtg ctttgggcgg ctgaaagaat atcgccgcat cgccacgcgt
       61 tacgacaaaa cggcgagaaa ttacctggcg atggtgaaac tgggctgcat ccgactcttt
      121 tatcaacgct tacgtaatta agggacacag cctaaatagc tgcgcggaat agtagatccc
      181 tttgagggaa cttagcccgg attgtgcgat ctgatcaatc gccaaatcaa aacaaatcac
      241 caaccggact gagcaatgcc gatcatagca ccaatttccc gtgacgaacg acgcctgatg
      301 cagaaagcca tccataaaac acacgataaa aattatgccc gcagactgac tgccatgctg
      361 atgctgcacc ggggcgaccg tgtcagcgac gttgccagaa cgctctgctg cgcccgttcc
      421 tctgttggac gctggattaa ctggttcacg cagtcgggtg ttgagggact gaaatcatta
      481 cctgccgggc gtgcccgtcg ctggccgttt gagcatatct gcacactgtt acgtgagctg
      541 gtaaaacatt ctcccggcga ctttggctac cagcgttcac gctggagtac agaactgctg
      601 gcaataaaaa tcaatgagat aaccggctgc cagttaaatg ccggaaccgt tcgccgctgg
      661 ttgccgtctg
//
LOCUS       DAZSIO010000464          669 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZSIO010000464 DAZSIO010000000
VERSION     DAZSIO010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.471"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..189
                     /locus_tag="ACHX5T_004496"
     CDS             <1..189
                     /locus_tag="ACHX5T_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469356.1"
                     /translation="SVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHN
                     KGNKNNSTGYNEQSEEEC"
     gene            183..>669
                     /gene="spa33"
                     /locus_tag="ACHX5T_004497"
                     /gene_synonym="spaO"
     CDS             183..>669
                     /gene="spa33"
                     /locus_tag="ACHX5T_004497"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HFR8469357.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TL"
BASE COUNT          211 a           99 c          123 g          236 t
ORIGIN      
        1 tctgttagta tttcagtgga gtctggttct tttgttctaa aaccgtcaga tcaatttgta
       61 ggaaataaac ttgacttaat tttgaaacaa gatgctgagg gtaattacag atttgatagc
      121 agtcaacata ataaggggaa taaaaataat agtacaggat ataatgaaca gagtgaagaa
      181 gaatgctaag aattaaacat tttgacgcta acgaaaaact acagatttta tatgcaaagc
      241 aactctgcga gcgtttttcg attcagacat tcaaaaataa atttacaggc agtgaaagtt
      301 tagtcactct tacttccgtg tgtggggatt gggtaattcg tattgataca ttatcttttt
      361 tgaaaaaaaa atacgaggta ttttcaggat tttctacaca agaatcttta ctgcatttat
      421 caaaatgtgt ctttatagag tcgtcatctg tattttcgat tccagaactg tctgataaga
      481 ttactttccg gatcacgaat gaaatccagt atgcaactac tgggagtcat ttatgctgtt
      541 tttcctcttc tttaggtatt atttattttg acaagatgcc ggtattacgt aatcaagttt
      601 ctcttgactc attgcatcat cttttagagt tttgcttagg ttcatctaat gtaaggctgg
      661 ctactttaa
//
LOCUS       DAZSIO010000465          669 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZSIO010000465 DAZSIO010000000
VERSION     DAZSIO010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.472"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..417)
                     /locus_tag="ACHX5T_004498"
     CDS             complement(<1..417)
                     /locus_tag="ACHX5T_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFR8469358.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQ"
     gene            complement(427..>669)
                     /locus_tag="ACHX5T_004499"
     CDS             complement(427..>669)
                     /locus_tag="ACHX5T_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFR8469359.1"
                     /translation="GALQGQANAGQTYANNMSSIAQQSAALAAANANKPSSLQTAISG
                     GTSGAIAGAGLASLLGTSTPWGAGIGAGIGLLGSLF"
BASE COUNT          161 a          194 c          157 g          157 t
ORIGIN      
        1 ttgcattgct tctggagacg atgacgcaag gcgtgcgcca gccgctaagg tgccgataga
       61 attacgctgc tcttcatcaa tgaatcccat gcctttacga acggattcaa tctggtctgg
      121 atattgagta gccaactgac gcaaagcacc gcgatcacca gacgcataag cattagcgta
      181 cgcctgctga aattctttct gccgctgagc ctgcttttcc tgctgaaaaa cacccgcaat
      241 acctgaaaga ccttgcaaag cagtcagccc aacattgtta gcgcctgaac gctcaatatc
      301 attgttctgc ctgataagct gaagcgtatt gccgatgtca tttacgctcg gagcgtttga
      361 gttgacgccg ccgataccag ccaacaatcc gccgtttgtt ccttgccaag tagccatgat
      421 tcccccttaa aacaacgagc caagcaatcc gataccagca ccaataccag cgccccaagg
      481 cgttgatgtt cccaaaaggc tggcaagacc tgcaccggca atcgcaccag acgtgccacc
      541 gctaattgca gtctgaagac ttgatggttt attggcatta gcagcggcaa gagctgcgct
      601 ttgctgtgca atgctgctca tgttgttggc gtacgtctgc ccggcgtttg cctgaccttg
      661 cagcgcacc
//
LOCUS       DAZSIO010000466          662 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZSIO010000466 DAZSIO010000000
VERSION     DAZSIO010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.473"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
BASE COUNT          175 a          105 c          181 g          201 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tg
//
LOCUS       DAZSIO010000467          659 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZSIO010000467 DAZSIO010000000
VERSION     DAZSIO010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.474"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            25..570
                     /locus_tag="ACHX5T_004500"
     CDS             25..570
                     /locus_tag="ACHX5T_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR8469360.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSSYNAIEWPASPEGSS"
     gene            594..>659
                     /locus_tag="ACHX5T_004501"
     CDS             594..>659
                     /locus_tag="ACHX5T_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR8469361.1"
                     /translation="MSVVISGALTDGAGIPMSGYHI"
BASE COUNT          194 a          141 c          168 g          156 t
ORIGIN      
        1 gtataacgta gcgagtattt aacaatgttg catttaaaaa atattactgc gggtaatccg
       61 aaaacagcag aacaatatca gatgacaaaa cgatattcgg tcacctggct tttttcagaa
      121 gacggaaaaa actggtatga agagctgaag aatttcgcca gcgacacaat aaaaatagct
      181 tacaccggag atggtcgcgt ggtgtgggtc ggtaaggatg tgacaggcat cgagccacgc
      241 aatgccagcg ttatcgaagt tccggatatt accgctaacc gacggattac cgcgccgggt
      301 tactggtttt accgcaatga tgaatttgtc tttgactaca gactcaaagc ggaagatgag
      361 cgtgatgccc ttctggctca ggtcagtgcc cggacagggg aatgggaaga agacctgctg
      421 ctggggttaa tcagcgacga agataaagaa aagctgaaag cctgtcgtat ttacgcgaaa
      481 tcgctgcagg cgatggattt cagcaccatc actgataaat cctcatacaa cgccattgaa
      541 tggcccgcct ctccggaagg ttcttcctga tttaatttat cgcgagaaaa acaatgtctg
      601 tagtgatatc aggtgcgctg actgatggcg caggtatccc catgtccgga taccatatt
//
LOCUS       DAZSIO010000468          658 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZSIO010000468 DAZSIO010000000
VERSION     DAZSIO010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.475"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            31..>658
                     /gene="icsB"
                     /locus_tag="ACHX5T_004502"
     CDS             31..>658
                     /gene="icsB"
                     /locus_tag="ACHX5T_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HFR8469362.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLD"
BASE COUNT          253 a          126 c          111 g          168 t
ORIGIN      
        1 aatcaccaag taatggagag ttaataaagt atgatcctca aaattagcaa tttcattgac
       61 gcaagcaata caaaagggcc tatacgcgtt gaagatacag agcatggacc tatattgata
      121 gcacagaaat tcaacctaaa ggatctcttt ttcagaacat taagcactat taatgcaaag
      181 ataaattcac agatattaaa tgaacaacta aaaaattatc gactagagaa tcaaaaatcc
      241 ttattgcttt tcctcaatac attagctagc gaaaaatctg cagaaagtgc gtttgccgcc
      301 tatgaagcag ctaaaaactc aattcaacac tctttcacag gtagagacat caaattaatg
      361 ttgaataccg cagagcgttt tcatggcatc ggtacagcca aaaatcttga aagacattta
      421 gtttttcgtt gctggggaaa tagaggcata acccatttag ggcatactag tatcagtata
      481 aaaaacaatt tactacagga accaactcat acctatctca gctggtatcc cggcgggaat
      541 gttacaaaag atacagaaat aaactacctt tttgaaaaac gctcaggtta cagtgtcgat
      601 acctataagc aagacaaact aaatatgatt tcagaccaaa cagccgaaag acttgatg
//
LOCUS       DAZSIO010000469          658 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZSIO010000469 DAZSIO010000000
VERSION     DAZSIO010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.476"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            140..646
                     /gene="mug"
                     /locus_tag="ACHX5T_004503"
                     /pseudo
     CDS             140..646
                     /gene="mug"
                     /locus_tag="ACHX5T_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT          160 a          147 c          188 g          163 t
ORIGIN      
        1 ctgagctaag gggccatggt agcggattat aaagtaactc cgtgccgcaa tccagccatt
       61 accgcgcgcc tgctgttttt ataaacaatg tattttcaat ccgttatact tttcctggtg
      121 atgttagaaa ggagtaaata tggttgagga tattttggct ccagggttac gggtcgtgtt
      181 ttgcggtatc aaccctgggc tttcatccgc cgggactggt tttccctttg ctcatccggc
      241 aaatcgcttc tggaaggtga tatatcaggc cgggtttacc gaccgtcagt tgaagccgca
      301 ggaggcacag catctgctgg attatcgttg tggcatcacc aaactggtag accgtccaac
      361 ggtgcaagcc aatgaagttt caaagcagga gctgcacgca ggtgggcgta agctgattga
      421 aaaaattgaa gattatcagc cgcaggcgtt ggctattctg ggcaaacaag catatgaaca
      481 gggattcagc tagcgcggtg cacagtgggg gaaacaaacg ctcaccattg gttcgacgca
      541 gatttgggtg ctgccaaatc ccagcggttt aagtcgcgtt tcactggaga aactggttga
      601 agcgtatcgc gagctggacc aggcgctggt agtgcgtggg cgataaaaaa cgtcaccg
//
LOCUS       DAZSIO010000470          656 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZSIO010000470 DAZSIO010000000
VERSION     DAZSIO010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.477"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>656
                     /locus_tag="ACHX5T_004504"
     CDS             <1..>656
                     /locus_tag="ACHX5T_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469363.1"
                     /translation="NTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQPINSTFSGEG
                     YNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETISSTPLTQQTQS
                     ILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSVQTVSSDNKNF
                     AGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNMTGQDMSKLVL
                     T"
BASE COUNT          250 a          136 c          108 g          162 t
ORIGIN      
        1 aatacatatc acggcgatat ttataactgc acaacaaata ccgataatgt taaaacgcca
       61 gacacaccga agtggccttg tgataattgg gaagaacaac aacccattaa ttccacattc
      121 tctggagaag gatacaaccc tgaacaattt gatttagcgc aacaacaact ccaacaaatt
      181 aatgcatgcc acacgaacac cacatataca aatgcagact actcaaaagt tgtggttcaa
      241 cttgtaagtc ttattaataa cattgaaacg ataagttcga caccacttac gcaacagaca
      301 caatcaatac ttaatcagat tcataacatt agatacgaaa aggataagga ttctgtatgt
      361 caaatcattg ttgtcgttaa tccagaaccc aacaatccaa tcataactaa aattctcgta
      421 gaagaaggaa tacccgaaag attcagtgtc caaaccgttt cttccgataa taaaaatttt
      481 gctgggcaac gagctgactt accaactgat ataagagaca ttcaaagctt gtacttaaaa
      541 atggcgaagc tgtatattga gcacagtaaa aatgaaaata ggatggaagc ccttgctgga
      601 tgcgacttta ttgatttcaa catgacaggg caagatatgt ccaagctagt cctaac
//
LOCUS       DAZSIO010000471          656 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZSIO010000471 DAZSIO010000000
VERSION     DAZSIO010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.478"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>656
                     /locus_tag="ACHX5T_004505"
     CDS             <1..>656
                     /locus_tag="ACHX5T_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469364.1"
                     /translation="VLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRNLPSGVVP
                     AVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHR
                     YGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELS
                     WWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPL
                     P"
BASE COUNT          144 a          179 c          189 g          144 t
ORIGIN      
        1 gttctggttc cggaactcgg gctggtggtc cttaagcccg gtcgtgaatc catgccggta
       61 ttccacaata cccgggtact ggtggagccg gaaccgaaaa gcatgcgtaa tctgccgtcc
      121 ggggtcgttc ctgccgttcg ccagccgcta gtggaagaca aaacattgct gccgtttttc
      181 agtaacgcac gggtgattcg tgctgctggt ggtgctggcg cattgtctga ctggctgttg
      241 cgccatatta aatcctgcca gtggccacac ggcgattatc atcacagcga aaccgtcatt
      301 caccgttatg gtaccggcgc aatggtattg tgctggcact gcgacaacca gctgcgtgac
      361 cagacatccg aatcactcga gcaacttgct catcaaaacc tgtcagcatg gatgattgac
      421 gtcatcggtc acgcaataag cggtacgcag gagcgtgaat tatctctggc tgaattatcc
      481 tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg aagcggtatt acgtcgttcg
      541 ctggggttgc gtgcggaaaa aatccgctca atgtaccgtg aaagcgacat cgtgccggga
      601 gagcagaccg ccaccagcat actgaagcag cgcacaaaaa atcttgcgcc gctgcc
//
LOCUS       DAZSIO010000472          656 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZSIO010000472 DAZSIO010000000
VERSION     DAZSIO010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.479"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(151..>656)
                     /locus_tag="ACHX5T_004506"
     CDS             complement(151..>656)
                     /locus_tag="ACHX5T_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR8469365.1"
                     /translation="PVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDL
                     KELRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDP
                     STPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKL
                     VERLMQL"
BASE COUNT          173 a          160 c          144 g          179 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc gtaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttgccgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaa
//
LOCUS       DAZSIO010000473          641 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZSIO010000473 DAZSIO010000000
VERSION     DAZSIO010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.480"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..143)
                     /gene="csgC"
                     /locus_tag="ACHX5T_004507"
     CDS             complement(<1..143)
                     /gene="csgC"
                     /locus_tag="ACHX5T_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HFR8469366.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLR"
     gene            complement(202..567)
                     /gene="csgA"
                     /locus_tag="ACHX5T_004508"
     CDS             complement(202..567)
                     /gene="csgA"
                     /locus_tag="ACHX5T_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFR8469367.1"
                     /translation="MPVHLSNNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHG
                     GGNGADVGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASN
                     SSVNVTQVGFGNNATAHQY"
BASE COUNT          166 a          146 c          155 g          174 t
ORIGIN      
        1 tcgcgcaggg acaatatttg tactctgcac agacaagatt gagtaagagt aacttcagga
       61 ataatggtgt acatatcccc ttgctgggtc gtattaaagg ttatctggct ggaaagtgcc
      121 gcaaggagta ataacgcatt catattcttc tcccgaaaaa aaacagggct tgcgccctgt
      181 ttctgtaata caaatgatgt attaatactg atgagcggtc gcgttgttac caaagccaac
      241 ctgagtcacg ttgacggagg agttcgatgc agtctggtca actgcagcac cgttgccacc
      301 accgaactgt ttaacagtca tttcagaatt tttgccgttc cactgatcaa gagtggcgct
      361 gttaccgaag ccacgttggg tcagatcgat tgagctgtca tctgagccct gaccaacatc
      421 tgcaccatta ccaccgccat gctgggtaat agtcaagtca gagttacggg catcagtttg
      481 cagagcaagt gcagagttac cgccaccgta ctggtaaatg ttcagctctg aatttgggcc
      541 gctattatta ctgaggtgta ctggcaatag cggacactac catttgttct tttttaagca
      601 gccatctgat gatatttttc cctgaaggct gccggggaga t
//
LOCUS       DAZSIO010000474          640 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZSIO010000474 DAZSIO010000000
VERSION     DAZSIO010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.481"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(1..107)
                     /locus_tag="ACHX5T_004509"
                     /pseudo
     CDS             complement(1..107)
                     /locus_tag="ACHX5T_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            105..188
                     /gene="tnpB"
                     /locus_tag="ACHX5T_004510"
                     /pseudo
     CDS             105..188
                     /gene="tnpB"
                     /locus_tag="ACHX5T_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          178 a          130 c          164 g          168 t
ORIGIN      
        1 gaaggagccg atgacttttc gtggatttcc acggaacgcg agaagcagat tgttttacgt
       61 gccagccgct tgatgcgggt gcggagggtc aggttgttac gttcaagata tagcatgtgc
      121 tggatgaaga tcccttttct ggtcacctgt ttatctttcg tggccgacgg ggtgacactg
      181 ttaaaatgta atgaacgcat cccacacaat taaacacgga gtagtatgat ggttaaaagg
      241 gtgaaactgg caggtgtgga gcctcgcagc tttccggcat catactgccc aatacgggat
      301 gcataaagcg tcctcagact ggagactcca caaatgaaat atacaccggt tggcgttgat
      361 atggcaaaac atgtcattca gattcacttc atcaatgagc acatgtcagc gccagtgata
      421 taagacggta atggggatgc ggacgatttc ctggactctc agaagatgaa acatgtttac
      481 tgaagaacaa aaaattcgcg ctattgagct ttatttcaaa tatggtaaga agcttgaggt
      541 gtactggcaa tagcggacac caccatttgt tctttttcta agcagtcatc tgataatatt
      601 ttatcctgaa ggctgccggg gagatattcc ccagacgaga
//
LOCUS       DAZSIO010000475          635 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZSIO010000475 DAZSIO010000000
VERSION     DAZSIO010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.482"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            18..491
                     /locus_tag="ACHX5T_004511"
     CDS             18..491
                     /locus_tag="ACHX5T_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8469368.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          210 a          113 c          127 g          185 t
ORIGIN      
        1 gagatgaacc ctatgcaatg tcctggttaa tatctcagtg tgctcatcag tgcactgata
       61 ataaaaagac ggagacagat gccatttacg ataaagttcg gagttcttat ttgctttcat
      121 gtattctgaa aaaaaacaaa aatgttgggc ttattcttca tgcaccatca tttgtttctg
      181 tgtctgaaaa aatcgccaga attgtgatgg caaattactc ccgtaactgg agtaattccg
      241 agttggccag tgcggtttta atgagtgaat cttctttaaa aagaagaatg tacaaagagg
      301 tcggttctat aagtacattt gtacataaaa taaagcttac agaagctatc aggaaattac
      361 gaaggacaaa tactcccatt tctgtcatat catctgagtt aggttacagt tcaccatcgt
      421 atttttctaa agtcttcttt aaatatttaa agacataccc tcaaaacata aggaagaaaa
      481 atggtcggta atgtcaccaa ttaggaggac ttgcagagcc gcttttttac gatactgaga
      541 tcaaacagac ataatctatg cggagattta cagcagggca tggttcacaa atctgacagc
      601 gatgaattat cggcactgcg ggcagaaaat gctcg
//
LOCUS       DAZSIO010000476          633 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZSIO010000476 DAZSIO010000000
VERSION     DAZSIO010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.483"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..224
                     /locus_tag="ACHX5T_004512"
     CDS             <1..224
                     /locus_tag="ACHX5T_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HFR8469369.1"
                     /translation="EIKNISHVIDREVKSLAESIRQEISTFSGMNRIYLTGGGAELIY
                     PHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            224..619
                     /locus_tag="ACHX5T_004513"
     CDS             224..619
                     /locus_tag="ACHX5T_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR8469370.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
BASE COUNT          236 a          130 c          121 g          146 t
ORIGIN      
        1 ctgaaataaa aaacatttct catgtaatag acagagaagt gaaatctctg gcagaaagca
       61 tcaggcagga aatttcaaca ttctcaggaa tgaaccgaat ctatctcact ggcggaggag
      121 ccgagcttat ttaccctcac ataaagcaat atttcccgaa tctgaaagtg aacaaggtgg
      181 atgaacctca gtttgcattg gtgaaagcaa tggtgcatgc gtaatggaaa gcagtgatcc
      241 aaaaaaaaga aagaaagttg tggcatacct gcacccggca ctttatccgc aggacaacct
      301 gacacaacaa acaatagact ctctcccagt acaaatgcgg ggagatttct acagacaatc
      361 ccttatttgc ggagcagctc tttattctgt tgccccccga ctgttaacgc taatcagcgt
      421 ttttttcagt gagaaaatca ctgcagaaaa tctggtgaaa cttatagaac aaacaacagg
      481 atacacatcc acatcaattg atataagtgt gttaaaaaac atcatagagg catcttcaga
      541 aaacaaatca gagagcatca cttcaaaaga tgatttcgaa gaacaaacca gacgcaatct
      601 ttctatgtta aaaaaataat aaaagaagag cag
//
LOCUS       DAZSIO010000477          630 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZSIO010000477 DAZSIO010000000
VERSION     DAZSIO010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.484"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            5..472
                     /gene="ipgC"
                     /locus_tag="ACHX5T_004514"
     CDS             5..472
                     /gene="ipgC"
                     /locus_tag="ACHX5T_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HFR8469371.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            478..>630
                     /locus_tag="ACHX5T_004515"
     CDS             478..>630
                     /locus_tag="ACHX5T_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469372.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNI"
BASE COUNT          219 a          121 c          103 g          187 t
ORIGIN      
        1 ccttatgtct ttaaatatca ccgaaaatga aagcatctct actgcagtaa ttgatgcaat
       61 taactctggc gctacactga aagatattaa tgcaattcct gatgatatga tggatgacat
      121 ttattcatat gcttatgact tttataacaa aggaagaata gaggaagctg aagttttctt
      181 caggttttta tgtatatacg acttttacaa tgtagactac attatgggac tcgcagctat
      241 ttatcagata aaagaacagt tccaacaagc agcagacctt tatgctgtcg cttttgcatt
      301 aggaaaaaat gactatacac cagtattcca tactggacaa tgccagcttc ggttgaaagc
      361 ccccttaaaa gctaaagagt gcttcgaact cgtaattcaa cacagcaatg atgaaaaatt
      421 aaaaataaaa gcacaatcat acttggacgc aattcaggat atcaaggagt aattattatg
      481 cataatgtaa gcaccacaac cactggtttt cctcttgcca aaatattggc ttccactgag
      541 cttggagaca atactatcca agctgcaaat gatgcagcta acaaattatt ttctcttaca
      601 attgctgatc ttactgctaa ccaaaatatt
//
LOCUS       DAZSIO010000478          629 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZSIO010000478 DAZSIO010000000
VERSION     DAZSIO010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.485"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..152
                     /gene="mxiI"
                     /locus_tag="ACHX5T_004516"
     CDS             <1..152
                     /gene="mxiI"
                     /locus_tag="ACHX5T_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HFR8469373.1"
                     /translation="MEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVE
                     TLLKS"
     gene            158..>629
                     /gene="mxiJ"
                     /locus_tag="ACHX5T_004517"
     CDS             158..>629
                     /gene="mxiJ"
                     /locus_tag="ACHX5T_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HFR8469374.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPK"
BASE COUNT          205 a           98 c          132 g          194 t
ORIGIN      
        1 taatggagat ggtaagcaat ccagaatcat taaacccaga atctttggcc aagttacaga
       61 cgacgctctc aaattattca ataggagtat cattagctgg cacgttagca agaaaaacag
      121 tttcggctgt tgaaacttta ttaaagtctt aatttatatg attaggtata aaggttttat
      181 tttattcttg ttgctgatgt tgattggatg tgagcaacgt gaagagttaa tttctaattt
      241 atctcaaaga caggcaaatg aaataatatc tgtgctagaa cgccataata ttactgctag
      301 aaaagttgat ggaggtaaac aggggatctc ggtacaagtc gaaaagggga catttgcatc
      361 ggcagttgat ttgatgcgca tgtacgattt gccaaatccg gagagagttg atatctcaca
      421 aatgtttcct acagattcat tagtgtcttc tccaagagct gaaaaggccc gtttatatag
      481 tgctattgag caacggctgg aacagtcttt agtttctatt ggtggtgtta tttcggcaaa
      541 aatacatgtt agctatgatc ttgaagaaaa aaatatatct tcaaaaccga tgcatatatc
      601 agtaatcgct atatatgact caccgaaag
//
LOCUS       DAZSIO010000479          619 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZSIO010000479 DAZSIO010000000
VERSION     DAZSIO010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.486"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..>619)
                     /gene="ipaH3"
                     /locus_tag="ACHX5T_004518"
     CDS             complement(<1..>619)
                     /gene="ipaH3"
                     /locus_tag="ACHX5T_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFR8469375.1"
                     /translation="RNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQ
                     NALISLPELPASLEYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINA
                     DNNQLTMLPELPTSLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHS
                     EETEIFFRCRENRITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          164 a          106 c          168 g          181 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 cgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgat
//
LOCUS       DAZSIO010000480          617 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZSIO010000480 DAZSIO010000000
VERSION     DAZSIO010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.487"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..525
                     /locus_tag="ACHX5T_004519"
     CDS             <1..525
                     /locus_tag="ACHX5T_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR8469376.1"
                     /translation="QKLSSLDNPTQIKDNATTGDSSAQFALGKYEFICANIDEGIKWL
                     TKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQAATLGSSNAQAEIGYLYLIGK
                     ELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDGVERNMEKARFHLSNAAEGGIE
                     PALKALNELEHQTK"
BASE COUNT          226 a          113 c          127 g          151 t
ORIGIN      
        1 cagaaattat cgtctttaga caatccaaca caaataaaag ataatgcaac aacaggcgat
       61 tccagtgctc aatttgcatt agggaaatac gaattcatct gtgcaaatat agatgaaggg
      121 attaaatggt taaccaaatc tgcagagcaa aaaaacactg atgcgatata ctttttagct
      181 acagcctaca aaggcaatgg tatacctgca aacaatgaaa aatacctagc atatctgcaa
      241 caagctgcaa cactgggcag tagtaatgcc caggcggaaa ttggctattt atatctcatc
      301 ggaaaagaac taccacagaa tcttcctgat gctggtgtct ggttcaaaaa agcagctgca
      361 caaggtaatt ttgccgcaca tttctatctg ggaaggatgt atcagaatgg tgacggcgtt
      421 gagagaaata tggaaaaagc caggtttcat ctatctaatg cggcagaagg tggaatagaa
      481 cctgcgctta aagcattaaa tgaacttgaa catcaaacaa aataaagcgc tcagggacca
      541 cgtaaggttc atcaaacaga aaaggatata gtttgcgatg aaagcgaaat ccactttgtt
      601 attaatgatt ttatctg
//
LOCUS       DAZSIO010000481          616 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZSIO010000481 DAZSIO010000000
VERSION     DAZSIO010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.488"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..204
                     /locus_tag="ACHX5T_004520"
                     /pseudo
     CDS             1..204
                     /locus_tag="ACHX5T_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145474.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(270..>616)
                     /gene="istB"
                     /locus_tag="ACHX5T_004521"
     CDS             complement(270..>616)
                     /gene="istB"
                     /locus_tag="ACHX5T_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HFR8469377.1"
                     /translation="STAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQEEAKLFFQV
                     IAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLDRILHHSHIVQIKGESYRLRQK
                     RKAGVIAEANPE"
BASE COUNT          153 a          144 c          153 g          166 t
ORIGIN      
        1 atgacaggcg aaaatcgtgg ccggggaagg ccgcgaaaga cagaaagtac ctatgcagat
       61 actcgtaatg atctgatccg cagtggcctt gaactgctga cgcagaatgg ttttcttgcg
      121 acgggagtcg atgccattgt taaaaatgcc aatgtcccta aaggctcatt ttattactac
      181 cttaagagta aagaggatta tgctgtcaac gacggatgaa aagtgatcca cttatatctc
      241 caccaacggc ccaatattga tccaccgttt tactcaggat tagcttctgc tataaccccg
      301 gcctttcgtt tctgtctgag tcgatagctt tctcctttga tttgaacgat atgtgagtgg
      361 tgtaagatac ggtccagcat cgctgaggtc agtgctgcat caccggcgaa cgtttgatcc
      421 cactgcccga acggcagatt ggatgtcagg atcattgcgc tcttttcgta acgtttagcg
      481 atgacctgga agaacagctt tgcttcttcc tgactgaacg gcagatagcc tatttcatca
      541 atgatgagca ggcggggggc cattactcca cgctgaagcg tcgttttata acggccctga
      601 cgttgtgccg tagata
//
LOCUS       DAZSIO010000482          609 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZSIO010000482 DAZSIO010000000
VERSION     DAZSIO010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.489"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(29..397)
                     /locus_tag="ACHX5T_004522"
                     /pseudo
     CDS             complement(29..397)
                     /locus_tag="ACHX5T_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          177 a          109 c          139 g          184 t
ORIGIN      
        1 ttgtctggag attcaggggg ccagtctaat cagttgtcat gcactcggaa taaatcatac
       61 cgtgaatagt gctgtttttg attgtcagac tattggcgtt ggtattggta gaaataccgt
      121 cgtccagata gtaggtagaa atcacgccgt taacggtagc gttattaatg accgggtaga
      181 tatcgccgtt gtaatagcta tcagcggcat agttgttcca gcctacataa ccatcatagt
      241 aaacgtcatc agcataggtg gcgtcgttgt agtgatggaa agtattgtaa gtttgaccag
      301 aaatatcgtt ggtcgctgca ttagcctgag aggtgatagc cagagtgcaa gccaatgcta
      361 attgtgatac tacaagtttc tttttccagg agtgcatttg tcatccctcc tcagggacgt
      421 aataagttga tccatcaatt atcaatgcat agaaaacata ttatgtttcg cgctaacgat
      481 tatgctgcgt tacagacgaa tggttcaatt attctttccc ctaagtccga atgtggacat
      541 atataattta tggagaaaaa cacgcatcaa attgataaaa ggacgatatt tcagtatgtt
      601 gcagataat
//
LOCUS       DAZSIO010000483          596 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZSIO010000483 DAZSIO010000000
VERSION     DAZSIO010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.490"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..199
                     /locus_tag="ACHX5T_004523"
                     /pseudo
     CDS             1..199
                     /locus_tag="ACHX5T_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          177 a          134 c          117 g          168 t
ORIGIN      
        1 cccacctgcc gggctaatcc accactccgc tcgaggttca cagtactgcg catacgatta
       61 ccgggtcata caggagcagt ctggtctgaa aacatcaatg tcgcgtaaag gtaactgtta
      121 cgacaacgct ccgatggaaa tcttctgggg aacgctgaaa aatgaaagcc tgagccacta
      181 tcgttttaag agccgggata tttcgtcagc atacgggaaa actgattgag gtcggcctca
      241 gttttgaggc ctttagccag ttcagccgca agggctttaa gcttcttttc gtccataatt
      301 tacctgtctc cgttgttgga gtaaacatat caaaaacagg caattacaca atttaaatta
      361 cagtctcaaa acaaacccaa cggtcaatat agtttaccag caagcttact agcaaagtgc
      421 taatcatttg gtatctttcg tgctattctc cttttacgta agtacttaag gggaacgtat
      481 ttactacttt taaagaaagc tccgttacta tctgaaatgc aatgagataa taaacgatta
      541 ctctcggtta gtatctcatg atcatttaca gactttgcat aatcgcgaat actata
//
LOCUS       DAZSIO010000484          595 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZSIO010000484 DAZSIO010000000
VERSION     DAZSIO010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.491"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            29..>595
                     /gene="virB"
                     /locus_tag="ACHX5T_004524"
     CDS             29..>595
                     /gene="virB"
                     /locus_tag="ACHX5T_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HFR8469378.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELN"
BASE COUNT          222 a          110 c          112 g          151 t
ORIGIN      
        1 aaatcaatat gaataaacag ggtgtgatat ggtggatttg tgcaacgact tgttaagtat
       61 aaaggaaggc caaaagaaag agtttacact ccattctggt aataaagttt cctttatcaa
      121 agccaagatt cctcataaaa ggatccaaga tttaaccttc gtcaaccaaa aaacgaatgt
      181 acgcgatcaa gaatccctaa cagaagaatc attagccgat atcataaaaa ctataaagct
      241 acaacaattc ttccctgtaa taggaaggga gattgatggt agaattgaaa ttctggatgg
      301 cactcgtaga agagcatctg caatatatgc aggagcagat cttgaagttc tatattcaaa
      361 agaatatata tctactcttg atgccagaaa actagcaaac gatatacaaa cagcaaaaga
      421 gcatagcatc cgagaacttg gtattggtct taattttctg aaagtatcag ggatgtccta
      481 taaagacata gccaaaaaag agaatctgtc tcgcgcgaaa gtcactcgtg cctttcaggc
      541 agcaagcgtt ccacaggaaa taatatctct atttccaatc gcgtcagaac ttaac
//
LOCUS       DAZSIO010000485          593 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZSIO010000485 DAZSIO010000000
VERSION     DAZSIO010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.492"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            23..593
                     /locus_tag="ACHX5T_004525"
                     /pseudo
     CDS             23..593
                     /locus_tag="ACHX5T_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004243150.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
BASE COUNT          171 a          108 c          132 g          182 t
ORIGIN      
        1 ttgttataga gaagcagtcg atattcatag ctcacttgag ctttcattta tattgcataa
       61 atatcatttg cgtgtagatg agaagaaatc tcgaaatgta ttcagcgcca tacgtgatga
      121 gctcatcctt gatggggatt cacgacaaaa cttagctact ttctgtcaaa catggcttga
      181 tgatgaaatc cgtgagttgc ttaatttagc tattgataaa aacatgattt ataaagatga
      241 atatccacaa atggcggaac ctgaaggtcg ctgtgtgcgt atgctttccg attcgtggag
      301 tttccccgat tcgcgacaca ccttaggttg ctcaaccatt ggttcatctg aagctgctat
      361 gctcggccgc ttggcgttaa aatggcagtg gtgtaaaaaa cgcgaagtac aaggtaaatc
      421 aaccgaacct gatttgcgat cctgtgcaaa tatgctggca taaattctgc cgttactgtg
      481 atgtgaaatt gtgtgaaatt ccacttgatg atcatcacct gataatgaat gctaaagagg
      541 ttttgaagca tgtcgatgaa aacactattg gtgtcgttac gactttaggt att
//
LOCUS       DAZSIO010000486          592 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZSIO010000486 DAZSIO010000000
VERSION     DAZSIO010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.493"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            281..>592
                     /gene="virA"
                     /locus_tag="ACHX5T_004526"
     CDS             281..>592
                     /gene="virA"
                     /locus_tag="ACHX5T_004526"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFR8469379.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DG"
BASE COUNT          209 a          113 c           86 g          184 t
ORIGIN      
        1 gttctttctg tacgcttgcc caaagtttaa ccactttgtt tttctataca cccaatggtt
       61 ctatctgccc cttgatgtta atccccaaag ccaataacat gaatgtattc atatcacaac
      121 tttcctacca gactctccaa tgcaataact gcataattgt ttttagtgga aatgtatcgt
      181 tttcttaaag agaagaataa cattccattt tattatgttc ccaatttaat tatattaata
      241 tatattggca ttaataggaa aatacatcag gagaaatcaa atgcagacat caaacataac
      301 taaccatgaa agaaatgact catcatggat gtcaactgtc aaatcaacta cagaagtaag
      361 ttggaataaa ttgagttttt gtgacatttt actgaaaata ataactttcg gcatatattc
      421 tccacacgaa actctagccg aaaaacatag cgaaaaaaaa ctaatggata gtttctcacc
      481 atcactatca caagataaaa tggacggaga gtttgcccac gcaaacatag atggcatttc
      541 aatacggcta tgccttaaca aaggcatatg ttcggttttt tatcttgatg ga
//
LOCUS       DAZSIO010000487          589 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZSIO010000487 DAZSIO010000000
VERSION     DAZSIO010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.494"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..529)
                     /gene="mxiE"
                     /locus_tag="ACHX5T_004527"
     CDS             complement(<1..529)
                     /gene="mxiE"
                     /locus_tag="ACHX5T_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HFR8469380.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYA"
BASE COUNT          192 a          123 c           82 g          192 t
ORIGIN      
        1 atgcataccc attattcatg gcagctgaag tgattgtttg attgtgcaaa aatacatcta
       61 ataaaccatt tactaaccgc caagtgttca gttgttcttt gacttttgcc cctaaagctt
      121 ttctacataa agatctaaaa taagcctccg acacaccata atgctctgtc agactcttta
      181 ttcttacctc cttttctttc tcaatagttc tcagaagata aagaaccaaa tgatatgact
      241 cagtcattcg tatcatagcc agtgcattac ttacattctg gtcagatgaa aagatgaatc
      301 ttaacattaa cttatcaagc atcgattcgg tattacaact atcccgaaga tcattctcat
      361 gatagaaatg aatacatttt tcagcatggc tatgctctac tagagcacca ctgaaatagt
      421 cccaaaaagc aagaaatttt gcacaactat taatgttaaa gcgataatga tagttcacat
      481 tatcactgaa ttttatattt tgatttcgga caaaaaaaaa cccttccatg ctggagccag
      541 ctcaattatg ttacgttctt tatcttttac aagctcaacg ttaactggt
//
LOCUS       DAZSIO010000488          582 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZSIO010000488 DAZSIO010000000
VERSION     DAZSIO010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.495"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(141..>582)
                     /locus_tag="ACHX5T_004528"
     CDS             complement(141..>582)
                     /locus_tag="ACHX5T_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR8469381.1"
                     /translation="GGKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLR
                     DMINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELL
                     NDAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          148 a          163 c           99 g          172 t
ORIGIN      
        1 caccagcacg tttaacgtcc tgtgccgttt ttcaggtata aaaaaaaccg cataaagcgg
       61 gctctttcaa atgtccatgt ctgctattcg cctcgcggta cagctttgcg aagcgtacaa
      121 gaattgaagc agtttttacg tcaaaaagca ataacttttt tctctatacc aaaagccata
      181 accattggtt tgtacaaaat aaattctgcc acctttagcc aatgctcaat gcgtctttca
      241 caggttctta aactccattt cgggtgtgca tcattcagca gttcagccat tttgcatttg
      301 gtcatccccc gcccctcata ccgttgccga aggacgctaa tcaatcctgg atgctctgcc
      361 agcacctcac ttatgacccg atcaatacat aacgcctctg catcagtaca atgcgccagc
      421 cagctctttt gcttaccgtt aatcatatcc cgcaaaaaag cctcaagttc agacttgttc
      481 agacctgctt ttttcatcct ccggagcgcc tcgttaattg ccgtttttgt cagcttttta
      541 gaggtcaaca tcaggttaaa catattcccc gtcttaccgc ca
//
LOCUS       DAZSIO010000489          578 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZSIO010000489 DAZSIO010000000
VERSION     DAZSIO010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.496"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..242
                     /locus_tag="ACHX5T_004529"
     CDS             <1..242
                     /locus_tag="ACHX5T_004529"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469382.1"
                     /translation="VCRKKLRKRLTINTQLSHTDWQRKCFQEQVICHGKANQKYRLIL
                     VFKPFLLVSMIQRLSKKLGNSTTLGNYGLCPAALI"
BASE COUNT          186 a          116 c          123 g          153 t
ORIGIN      
        1 atgtctgtag aaaaaaactg agaaaaaggc taacaataaa tacacaactc agccatacag
       61 actggcagcg caaatgtttc caggagcaag tcatctgtca tggtaaagct aaccagaaat
      121 ataggttaat tttggtgttt aaaccttttt tacttgttag tatgatacaa aggctttcaa
      181 aaaagctggg aaattcaaca actcttggaa attatggact ttgtcccgcg gctttaatct
      241 gacggccgcg ttcctttttt ggctcaatta ttgacccctt ctctacgcaa cttcagttcc
      301 cgccagcaac tttgcggcag tttcatagga tcaatgtcta aaagaataat ggtgaccgat
      361 aagaaaacga ctgaataact gtagattttc gctcgaaacc ttcctgtcag ctccatagcg
      421 aatcaagcgc tgaatgtcac agtatcgaac agaaaacagt gacgatctaa cccttcaaga
      481 atattctacg attgttctgt ttaggaaaag caaggcggga agtcgggaga taagtcattg
      541 ataaaatggc ggagagaggg ggatttgaac ccccggta
//
LOCUS       DAZSIO010000490          578 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZSIO010000490 DAZSIO010000000
VERSION     DAZSIO010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.497"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(12..578)
                     /gene="ospZ"
                     /locus_tag="ACHX5T_004530"
                     /pseudo
     CDS             complement(12..578)
                     /gene="ospZ"
                     /locus_tag="ACHX5T_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
BASE COUNT          175 a          118 c           85 g          200 t
ORIGIN      
        1 attaatatac attaagtaac aggcattcga gccttgattt gagaaagcct gtcattcatt
       61 tgttttatgt agctatctat ttcctctgat gcataactaa aaagttctcc catttcacga
      121 gcaataataa tctcagattt aatagacttt aatctctggc gactcatctc ttaacacttg
      181 cataatctct ctttttatat catatagttc tataaaccga tttgcccgta gttctccttg
      241 ggaagaatca tttctgaata caacaattat atacaaaaaa tcactatttt ctttagagaa
      301 gatcttacaa ctaatctctg ggagctcaca taatgcatgt aagtcaggat ggtacaaact
      361 atccacagaa aattgcccca taggacgttt tccagatata actgtcaacc agcttccttc
      421 cggtgtactg gcttcggctc tggaaatagg ctgaaaatct tccggacatg gagaatacat
      481 tgtcttcata taattaagtc gtctggattt ctcattaata aactcctcta tttttaattg
      541 tgccatactt ctggttatac cagagatata cttcttgt
//
LOCUS       DAZSIO010000491          566 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZSIO010000491 DAZSIO010000000
VERSION     DAZSIO010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.498"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..297
                     /gene="pliG"
                     /locus_tag="ACHX5T_004531"
     CDS             <1..297
                     /gene="pliG"
                     /locus_tag="ACHX5T_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR8469383.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="ACHX5T_004532"
     CDS             complement(405..>566)
                     /locus_tag="ACHX5T_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR8469384.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DAZSIO010000492          563 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZSIO010000492 DAZSIO010000000
VERSION     DAZSIO010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.499"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..>563)
                     /locus_tag="ACHX5T_004533"
     CDS             complement(<1..>563)
                     /locus_tag="ACHX5T_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469385.1"
                     /translation="AVELFVKSSHTKTHSNDFFNNIQHAFKKKDWVSNYDSLLTLRES
                     FRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLRGSGFEYEEIPIT
                     INEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQAFGDVSFGYALL
                     EDVVSKLKRGKFELQIPGGGIKECDGW"
BASE COUNT          168 a          116 c           83 g          196 t
ORIGIN      
        1 accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat ttaccccgtt
       61 ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca ccaaacgcct
      121 ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc aaaatatcac
      181 aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa tccatgaatg
      241 agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg cctcgcaatg
      301 ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa ttatccatgg
      361 cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca atctgtgttg
      421 cgcaacgaaa ggactcccgc aaagttaaca gactatcata attcgaaacc caatcttttt
      481 tcttaaatgc atgctgaata ttattaaaaa aatcgtttga atgtgttttt gtatgcgatg
      541 actttacaaa taactccacc gca
//
LOCUS       DAZSIO010000493          559 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZSIO010000493 DAZSIO010000000
VERSION     DAZSIO010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.500"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..559
                     /gene="virF"
                     /locus_tag="ACHX5T_004534"
                     /pseudo
     CDS             1..559
                     /gene="virF"
                     /locus_tag="ACHX5T_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
BASE COUNT          196 a           85 c           98 g          180 t
ORIGIN      
        1 atgacggtta gctcaggcaa tgaaactttg actatcgatg aagggcaaat tgcttttata
       61 gagcgaaata tacaaataaa cgtctccata aaaaaatctg atagcattaa tccattttag
      121 attataagcc ttgacagaaa tttattatta agcattatta gaataatgga accaatttat
      181 tcatttcaac actcctattc tgaggagaaa agggggttaa acaaaaaaat attcctcctc
      241 tctgaggagg aggtttctat cgatttgttc aaatctataa aagagatgcc tttcggcaaa
      301 agaaagatct atagtttagc ttgcctttta tcagctgttt ctgatgagga agctttatat
      361 acttcgatat cgatagcttc ttctcttagt ttttctgatc agataaggaa gattgttgaa
      421 aaaaacatcg agaagagatg gcgtctttct gatatttcaa ataacttgaa tttatcagaa
      481 atagctgtta gaaaacgatt ggagagtgaa aaattaacat ttcaacaaat ccttcttgat
      541 attcgcatgc atcatgcag
//
LOCUS       DAZSIO010000494          559 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZSIO010000494 DAZSIO010000000
VERSION     DAZSIO010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.501"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>559
                     /locus_tag="ACHX5T_004535"
     CDS             <1..>559
                     /locus_tag="ACHX5T_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFR8469386.1"
                     /translation="APTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLHPK
                     IGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKA
                     TYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDT
                     ITRNHQCRSMWQLLKKVRHFMETVSP"
BASE COUNT          165 a          138 c          148 g          108 t
ORIGIN      
        1 tgcgccaact ctgcgtatcc gtgacccgca taaagatgaa aagatggcag caatccataa
       61 agcactggac gaatgcagcg cagagcatcc ggtcttttat gaagatgaag tggatatcca
      121 tcttcatccc aaaatcggtg cggactggca actgcgcgga cagcaaaaac gggtggtcac
      181 gccgggacag aatgaaaaat attatctggc cggagcgctg cacagcggga caggtaaagt
      241 cagctatgtg ggcggcaaca gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct
      301 taaagcgaca taccgtcgtg cgaaaaccat cacgctgatc gtggacaact acattatcca
      361 caaaagccgg gaaacacaga gctggctgaa ggagaacccg aagttcaggg gcatttatca
      421 gccggtttac tcgccatggg tgaatcatgt tgaacggcta tggcaggcac ttcacgacac
      481 aataacgcgt aatcatcagt gccgctcaat gtggcaactg ttgaaaaaag ttcgccattt
      541 tatggaaacc gtcagccca
//
LOCUS       DAZSIO010000495          558 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZSIO010000495 DAZSIO010000000
VERSION     DAZSIO010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.502"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..471)
                     /locus_tag="ACHX5T_004536"
     CDS             complement(<1..471)
                     /locus_tag="ACHX5T_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469387.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAP"
     gene            complement(464..>558)
                     /locus_tag="ACHX5T_004537"
     CDS             complement(464..>558)
                     /locus_tag="ACHX5T_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8469388.1"
                     /translation="LGEGQRYLSRCIKSSNDETYAICSEVLLYD"
BASE COUNT          181 a          113 c           91 g          173 t
ORIGIN      
        1 tggagcacat gtgttaatga tcaaccccgg gatactaaaa atatagacat tattaaactt
       61 gcttagcctc gcattgcctt tacctgttaa agatgcatcg agaaaattga gtctgaagtt
      121 ctcatccttt atctgatttt ccagtcccga aactttggcc ttagcattac ctgagatccg
      181 tccgttacta tctattttgt aggtaaaaaa atacgaatgg gaaaatttcc tcggtgtatc
      241 ttcatcagtt aagccaacaa tcgtaaaact accgctacca tcttttttta aaatgactga
      301 aaggttagca gagtagtata aagaatgatc tgttttagca tttcttgtta acgtcgacgt
      361 acattcaaga gacgttgtgc taccatcacg agccttccag agagcaaaaa aacacacctg
      421 tagcaatgtg aaaacaagta ttaatataaa atacattttc ttattagtca tataatagca
      481 cctctgaaca aattgcataa gtttcgtcat tagaggattt tatacatctt gataaatacc
      541 gctgcccttc cccaagca
//
LOCUS       DAZSIO010000496          556 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZSIO010000496 DAZSIO010000000
VERSION     DAZSIO010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.503"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(376..>556)
                     /locus_tag="ACHX5T_004538"
     CDS             complement(376..>556)
                     /locus_tag="ACHX5T_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFR8469389.1"
                     /translation="PSLKVDSLFISKDKRTYIKRKENKTNRTLIFTLSKINKTVQREI
                     DEAIRDIISRHLSSS"
BASE COUNT          165 a           90 c          100 g          201 t
ORIGIN      
        1 tggatttttt gtttatcacc atgtaaaaaa aattgagtag taaaagcgtt gactgctaat
       61 cctcgctgtt tttattgaaa cgattaaaaa tacgctgaat attttcggcc agtcactcat
      121 attcacataa aattttgacc atcatgccgc atcccttagt tgcacaattc attttcaatg
      181 atatgaatat tactaatttc accagataat tttctcgtgg tcagcagggt atgagttttc
      241 accgtaattt taaggaaaca cagtgcgtgg ggattgaata acagatagcc gacggaatta
      301 ccccaccggc tgaaagtcaa gttcaacgaa gatttttatt atctgaattg ggcagtttac
      361 atcagtgttc gatgtttatg aagacgatag atggcgagaa attatatccc gaatagcttc
      421 atctatttct ctctgaactg ttttgtttat tttagataat gtaaatatta gagttctgtt
      481 tgttttattc tcttttcttt ttatgtaagt ccgtttgtct ttagaaataa ataacgagtc
      541 aacttttagc gaaggg
//
LOCUS       DAZSIO010000497          553 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZSIO010000497 DAZSIO010000000
VERSION     DAZSIO010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.504"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..>553)
                     /locus_tag="ACHX5T_004539"
     CDS             complement(<1..>553)
                     /locus_tag="ACHX5T_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HFR8469390.1"
                     /translation="VEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKN
                     TDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKT
                     ATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVREDVMDQ
                     IVQEGSLEYATDVIIHLQQIDIKW"
BASE COUNT          133 a          120 c          135 g          165 t
ORIGIN      
        1 cccatttaat atcaatctgt tgaaggtgta ttattacatc agtagcatat tccagactgc
       61 cttcctggac gatctgatcc ataacatcct cacgcacttt atatcttgtg catttcattt
      121 caagtgccgt gagtacagcc attactggtg atggtagctg tgttgttttc aggccgtcga
      181 tctgactgat cgcttgggcg acggtgtttt gccattctaa agaacaggtg ctgaagtcaa
      241 atgtggctgt tttattatcc cgttgcgaca cgctacaggc taagcgacgt aattgggtca
      301 atttgtctgc ttgctgaaca ggaactccgg gaaagcggcg atgacttaac gtggtgtgta
      361 caacttcggg cggaaaaacg acagcaatat ctttcgccag ccaaggtaaa acagcatctg
      421 tatttttgtc agccgcacgg gtttctgcta cgaaagaggg ttgttcctga ataatggtgg
      481 ataagtccgc taccttgaga gagtccgtta ccgtttggga aggttgcaaa gaattatctt
      541 tcgcttgctc cac
//
LOCUS       DAZSIO010000498          550 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZSIO010000498 DAZSIO010000000
VERSION     DAZSIO010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.505"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>550
                     /gene="ospB"
                     /locus_tag="ACHX5T_004540"
     CDS             <1..>550
                     /gene="ospB"
                     /locus_tag="ACHX5T_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HFR8469391.1"
                     /translation="MAESWLPQSEPIVINNDDDALNAAAYFSVKKAKIKTVNDTDFKE
                     YNKVYILGHGSPGSHQLGLGSELIDVQTIISRMKDCGILNVKDIRFTSCGSADKVAPK
                     NFNNAPAESLSCILNSLPFFKEKESLLEQIKKHLENDESLSDGLKISGYHGYGVHYGQ
                     ELFPYSHYRSTSIPADPEHTVK"
BASE COUNT          182 a           97 c          102 g          169 t
ORIGIN      
        1 aaatggctga atcatggcta ccacagagtg aaccaatagt aataaataat gatgatgacg
       61 cattgaatgc tgctgcttat ttttctgtaa aaaaagcgaa aataaaaaca gtaaacgata
      121 ctgattttaa agagtataat aaggtttata ttcttgggca cggtagtcct ggttctcatc
      181 aattaggcct tggttcggaa cttattgatg tacaaacaat catttcaaga atgaaagact
      241 gtggtattct aaatgtgaaa gatatccgtt ttacttcatg cggctccgct gataaagtgg
      301 ctcctaaaaa ttttaacaat gcccctgctg aaagtctttc ttgtatcctt aactctctgc
      361 ctttttttaa ggaaaaagaa tctttgctag agcagataaa aaaacacctt gaaaacgatg
      421 agtcattgag tgatggtcta aaaatatccg gctatcatgg atatggagtt cactatggtc
      481 aagagctttt tccctactca cattatcgtt caacttcaat tcctgctgat ccggagcata
      541 cagtaaaaag
//
LOCUS       DAZSIO010000499          546 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZSIO010000499 DAZSIO010000000
VERSION     DAZSIO010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.506"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>546
                     /gene="ospC1"
                     /locus_tag="ACHX5T_004541"
     CDS             <1..>546
                     /gene="ospC1"
                     /locus_tag="ACHX5T_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HFR8469392.1"
                     /translation="PIFNTKDMKLGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDR
                     IINFVFQPEYHIPRMVSTENFKKVKIREISLEEAVTASNYEEINKQVTNKKIALQALF
                     LSITNQKEDVALYILSNFEITRQDVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLV
                     DVNTKFKKTNSGDCMLDNAIK"
BASE COUNT          202 a           78 c          100 g          166 t
ORIGIN      
        1 accaatattc aatactaaag atatgaagtt agggctcgga ttatacctga ttgactttat
       61 tagaaaaagt gaagaccaaa gcttcaagga gttttgctat ggaaaaaatc ttgcccctgt
      121 ggatctggat agaatcataa actttgtttt tcagccagag taccatatac ctaggatggt
      181 aagtacagaa aacttcaaaa aagttaagat tagagaaata tccttagagg aggctgttac
      241 agcatctaat tacgaagaaa ttaacaagca ggtcactaac aaaaaaattg ctctccaggc
      301 tctttttctt tcgattacta atcaaaaaga ggatgtcgcc ttatatatat tatctaattt
      361 tgagataact agacaagatg ttatttccat aaagcatgag ttgtatgata ttgagtatct
      421 acttagcgct cataattcaa gctgtaaagt acttgagtat tttatcaata agggattggt
      481 tgatgtaaac acaaagttca aaaaaactaa tagtggggat tgtatgttgg ataacgcaat
      541 aaaata
//
LOCUS       DAZSIO010000500          546 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZSIO010000500 DAZSIO010000000
VERSION     DAZSIO010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.507"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..229
                     /locus_tag="ACHX5T_004542"
     CDS             <1..229
                     /locus_tag="ACHX5T_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393504.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFR8469393.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
BASE COUNT          185 a          114 c           86 g          161 t
ORIGIN      
        1 ggaaagcaat tctgacagcg ccccctatac gttattaaaa aactatgctg acagatttaa
       61 aaaatattca gcagaagagc agtacgaaat tgattttcaa catcgactgg taaacagaaa
      121 ttgttattta gataaatatg gcgatgcacg aatcaatgct tcttatacac aattagaaat
      181 aaaacactca caacccaaaa atagagcatc cggcaaaaga gtgagttaac atcattgtta
      241 atcaattcga ccagtcagta aatatgctga ctggtcgaac ataatttgct aaaaagtcat
      301 cactgactga tgtcagtccc accatctgcc agttcaatct acgggtattt ccagcatatc
      361 gcggcacgcg attgctggtc tgttgatgta gtggattgta gttgctgact tttcttaccc
      421 tcagatttct gccccacttg tcaattcaat ctgccaataa ataacataat tatattttta
      481 ttcctcacag aaattccttt ttaaaaatag cgaacccact gtaaaaaaca ctttttgtat
      541 acctct
//
LOCUS       DAZSIO010000501          546 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZSIO010000501 DAZSIO010000000
VERSION     DAZSIO010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.508"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(25..>546)
                     /gene="rfbG"
                     /locus_tag="ACHX5T_004543"
     CDS             complement(25..>546)
                     /gene="rfbG"
                     /locus_tag="ACHX5T_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HFR8469394.1"
                     /translation="VIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPGCTYVIKKETLIE
                     FKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQHENNQVGANISFK
                     AKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILALSFWKLRRKKAD
                     KIYILLMLILNIF"
BASE COUNT          169 a          107 c           68 g          202 t
ORIGIN      
        1 ttcatattcg taaggtgatg ttttttaaaa tatgtttaaa atcaacatta ataaaatata
       61 aattttatca gcctttttcc gccttaactt ccaaaaactt aaagccaaga tcaaattgcc
      121 tatataacct ttacccaatt gattattcac aaagctatca tcagctaagg cattagctat
      181 tttagttact tcttttcgat accacttgtt tctcaccaaa ccaagccgtt tgtattttgc
      241 tttaaaacta atgtttgctc ccacttggtt gttttcatgc tgtctgtaca gcattgttgg
      301 ttttctatca atataccacg aataatttct cgtccgcgca aatgaatata aaaaccaatc
      361 atgcagacag atatcttgtg cagcattttt attatttatg atgaacttct taaattcaat
      421 aagcgtttct ttttttataa catatgtaca ccccggacct gctgcttcaa aaaaataatc
      481 gaattgagtt tgcgactgag ccttatcaac taaatttttt cgccctgatg gataatatgc
      541 aattac
//
LOCUS       DAZSIO010000502          545 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZSIO010000502 DAZSIO010000000
VERSION     DAZSIO010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.509"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            189..264
                     /locus_tag="ACHX5T_004544"
     tRNA            189..264
                     /locus_tag="ACHX5T_004544"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:222..224,aa:Ile,seq:cat)
BASE COUNT          144 a          103 c          138 g          160 t
ORIGIN      
        1 atagtgctaa ttattaacat ttgcttgttg actgcgagta tcgactttgc gaatggaggc
       61 ggtgtcgggt tttatgtcgc gtgtaatcac gacccaaggt gaatgggaac gcataaaaat
      121 ctgttattta catcataaga tcatcatgtt tattgcgtgg tttataagta ttgttattgc
      181 atacttaagg ccctttagct cagtggttag agcaggcgac tcataatcgc ttggtcgctg
      241 gttcaagtcc agcaagggcc accaaatcat cattcatgta tcaggaagag ttaagggtac
      301 aaggggtact cgatggcggt ctatatttca tgactaacgc catttcaagg aagaagtgcg
      361 gtgggtggtt tcgaatgagg gctggtgttt tggtacgtgg cacgccactg caatgtattt
      421 attcatcagt actcattcaa aagtacctgg aaaaaatgcc cgctatgcca agcgggcaaa
      481 ttgatcctac ccacgtaata tggacacagg cctaagcgag gttctggttt tcaaattgtt
      541 ccgga
//
LOCUS       DAZSIO010000503          545 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZSIO010000503 DAZSIO010000000
VERSION     DAZSIO010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.510"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>545
                     /gene="ipgB1"
                     /locus_tag="ACHX5T_004545"
     CDS             <1..>545
                     /gene="ipgB1"
                     /locus_tag="ACHX5T_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HFR8469395.1"
                     /translation="PQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQKNFGCPVN
                     INKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGVVLTSDNKK
                     NIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMDLFNTQMKD
                     LTNLVSSSVYDKIFNESTKVL"
BASE COUNT          206 a           98 c           91 g          150 t
ORIGIN      
        1 tccacaggtt gaattcgcga ttcctcgccc atcatttgat tcgttaagtc gcaataagct
       61 tgtaaagaaa atattatctg tttttaactt aaaacaacgc tttcctcaga agaattttgg
      121 atgcccggtg aatataaata aaatcaggga cagcgtcata gacaaaataa aagatagtaa
      181 ttcggggaat caactatttt gttggatgag ccaagagcga accacttatg tctcttcaat
      241 gataaacaga agtatagatg aaatggccat acacaatgga gttgttttga cctcagataa
      301 caaaaaaaac atatttgcag ctattgaaaa aaaattcccg gatataaaac ttgatgaaaa
      361 atcagcacaa acatctatca gtcatacagc actaaacgag attgcctcat ctggcctcag
      421 agcaaagatt ttaaaacgct atagcagtga catggatttg tttaacacac aaatgaagga
      481 tcttacaaat ctagtatcca gttccgtcta cgataaaata tttaatgaat caacaaaagt
      541 tttac
//
LOCUS       DAZSIO010000504          540 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZSIO010000504 DAZSIO010000000
VERSION     DAZSIO010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.511"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            80..460
                     /locus_tag="ACHX5T_004546"
     CDS             80..460
                     /locus_tag="ACHX5T_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFR8469396.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT          169 a          101 c          120 g          150 t
ORIGIN      
        1 ggaatgcaat aattttgcat tatctcgttg cttgtaatgc aaatatgcat tattgtcagt
       61 gtgtgacaat aacagagaga tgacgatggc gaccattaac gcacgtattg atgatgatat
      121 aaagaaccaa gcagatgaag tgctgaagct gatgaatatc agtcaaactc aggctattgc
      181 agctttttat cagtatatta ctgaacaaaa gaaactgcca tttgtgatca cctcaattgt
      241 aaaaacgccg catgatttat tacgtgaaag tacagacatg ttagctgaag ctcttgcagt
      301 gatttccaat ctacaggtgt ggactgaaca gcaggatggg attggaaaag caaagctaat
      361 ggagtattac cgccgcctcg acgcactgta ctgttgtgca aaagagaaaa taggtcttct
      421 ttcagataac cgggatgctg aactaggctg tgtcccttaa tcatctgagt tatagtaact
      481 gtctgtttac acaacatatt gaactatggc tcgctacgac cttcccgatg aggcatggac
//
LOCUS       DAZSIO010000505          529 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZSIO010000505 DAZSIO010000000
VERSION     DAZSIO010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.512"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            381..>529
                     /locus_tag="ACHX5T_004547"
     CDS             381..>529
                     /locus_tag="ACHX5T_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR8469397.1"
                     /translation="MSQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLRDIR
                     KMLEEL"
BASE COUNT          129 a          140 c          115 g          145 t
ORIGIN      
        1 tagggtctct ctataggtct tctctatagg tcttctctat ggtctctcta taggtctctc
       61 tagggtctcc tctaggcatt acccccagaa gctaactcta tggccctcta tggtcctcta
      121 tggccctgta cgtcattctt taggatgccc tgtagtttcg tatagcctga tgcctagaga
      181 gaccctagag agaccctaga gtggctccta gagagaccct cccagtagct ccgcacagct
      241 acatcgtcat catcatagta ttcaatctca aagtcattct gtttgcatcc tgttgtcctg
      301 ctggtctccc ttcgcttggg gactttgagt aggtcagtgg gggtgcgact tcagggtgca
      361 aacaaaaagg aaatcaagcg atgtctcaac aaccacaaca accacaacaa gaaatcagca
      421 tcattaacct cgaccagctt gtgtccatga cctcagttga aattgctgag ctgacaggca
      481 aggagcaccg aaatgtcctt cgtgatattc gtaagatgct cgaagaact
//
LOCUS       DAZSIO010000506          528 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZSIO010000506 DAZSIO010000000
VERSION     DAZSIO010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.513"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(1..227)
                     /locus_tag="ACHX5T_004548"
                     /pseudo
     CDS             complement(1..227)
                     /locus_tag="ACHX5T_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            211..469
                     /locus_tag="ACHX5T_004549"
                     /pseudo
     CDS             211..469
                     /locus_tag="ACHX5T_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          131 a          144 c          118 g          135 t
ORIGIN      
        1 gcacgcagcc atatcagctt ttcctgcctg gattcggtca tccatttatc gctgtgctgc
       61 cacaggaagc gagttccctt tgcctgacga cgactttcaa caggaagatg cggatgttca
      121 ttctggcggg ttttatccac cacctcaccc ggttgtttcg ccacatgaaa gcgatcgaag
      181 gctattttct caaccgcatt gggtaagtgg atgcgcgctg ctcttagttt gctgagccag
      241 tttagcattg ccttctacat gaccgatgcc tgagccggta taccgaactt tattgtcctc
      301 aaccagtcat gttataagca agaaatgcac tcagcggata gagcgacata atctgaacct
      361 gcggacacat cttaagcggc ttacccgcaa aaccatatgc ttctcaaagt ctgatgatat
      421 gcactacaag atcatcggct ggtatctgac aatcaaccac caccactaaa tctgcgtcac
      481 gacctgcaac tgagtctggt tgaacaggct gaggccctgc tgaagcaa
//
LOCUS       DAZSIO010000507          519 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZSIO010000507 DAZSIO010000000
VERSION     DAZSIO010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.514"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..246)
                     /locus_tag="ACHX5T_004550"
     CDS             complement(<1..246)
                     /locus_tag="ACHX5T_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HFR8469398.1"
                     /translation="MRDVQWFCPLTPVHTGTEIKPVEMPVTTITPVSDAGGLQDFIYW
                     RPDADRAGMEPVYVILNDPLDSGRFTRKQLNKNILNMQ"
BASE COUNT          134 a          127 c          117 g          141 t
ORIGIN      
        1 ttgcatgttt aagatatttt tatttagttg tttacgcgta aatcgcccgg aatccagagg
       61 atcattaagt atcacataaa caggttccat accagccctg tccgcatcag gacgccagta
      121 aataaaatct tgtagtccac cggcatcaga gacaggagta atcgtcgtta ccggcatttc
      181 gactggtttt atttccgtac ctgtatgtac tggtgtgagc ggacaaaacc actgtacgtc
      241 tctcatacac gctcgtaagc ggccctcacg gcccgctaac gcggagatac gccccgactt
      301 cgggtaaaac cctcgtcggg accactccga ccgcgcacaa aagcattttc atcgccgata
      361 gcgggtatgg cttggcaggg ccgggatggg tataggtaat ttggctatcg taaaaaaggt
      421 ggttaaaaat tacaacctgc aatgtaaatt ggctagttaa tttttggcta tttaaattga
      481 cttcttaacg gcgtttactg ttccattgct ccccaaccc
//
LOCUS       DAZSIO010000508          511 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZSIO010000508 DAZSIO010000000
VERSION     DAZSIO010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.515"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..511
                     /locus_tag="ACHX5T_004551"
                     /pseudo
     CDS             1..511
                     /locus_tag="ACHX5T_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISSfl7 family transposase"
BASE COUNT          127 a          128 c          141 g          115 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt a
//
LOCUS       DAZSIO010000509          509 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZSIO010000509 DAZSIO010000000
VERSION     DAZSIO010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.516"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>509
                     /gene="ospD1"
                     /locus_tag="ACHX5T_004552"
     CDS             <1..>509
                     /gene="ospD1"
                     /locus_tag="ACHX5T_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HFR8469399.1"
                     /translation="NMHLIIGSNTANENKGMKNNIINVTNTAISHAINEEKSGGGYSG
                     VSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGESLLAAEIPKEEKLE
                     VLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIKLLDVRDNEGLPGLF
                     LAAGKGNIE"
BASE COUNT          185 a           83 c          110 g          131 t
ORIGIN      
        1 aaaatatgca cttaataata ggcagcaata ctgctaatga aaataaagga atgaaaaata
       61 atatcattaa cgtgacaaat accgctatat cccacgccat caatgaagaa aaatcagggg
      121 ggggatatag tggtgtttct ttcagaaaat tggccaaaat acagaacata tccattccga
      181 caaagaataa taaggagtat aaccgccata atttgttttc attgatttgg catggaaatg
      241 ccgatgcagc gcgtaaatac ggtgaatcgc tgttggcagc cgaaataccc aaagaggaaa
      301 aactagaagt tcttgcagca cgaaataatg ctggggaatc tgctttgttc atagctcttc
      361 aagaaggtca ttccgctgcg attcaagctt atggagattt tattaaaact tttgatttat
      421 caccaaaaga aacgattaaa ctattggatg taagagataa tgaggggtta ccaggattat
      481 ttctggccgc agggaaaggg aatatcgag
//
LOCUS       DAZSIO010000510          509 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZSIO010000510 DAZSIO010000000
VERSION     DAZSIO010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.517"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(47..442)
                     /locus_tag="ACHX5T_004553"
     CDS             complement(47..442)
                     /locus_tag="ACHX5T_004553"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469400.1"
                     /translation="MNVVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFG
                     GMVRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEF
                     DIWNLNETWAFQEKLIFCEDESSLLNEVA"
BASE COUNT          148 a          122 c          108 g          131 t
ORIGIN      
        1 gctccttctg atgccattct atttcaggaa ggagtgtccg ttaaactcag gctacctcat
       61 ttaacagact tgattcatcc tcacaaaata taagtttttc ctggaatgcc catgtctcat
      121 tgaggttcca gatatcaaat tcgaagtcgt gataacgaaa acggatccca ccaaacttat
      181 taaaacgcag ttgtttgacg ggcagttcag ccaatgtctg gaagagttcc tcacgggagc
      241 gaccgatgac gatatcaagg tctgaagaga atccatcgac gccatacaag cctaagtcac
      301 gcaccatccc gccaaaaatc ctggccggac aggaacaggc cagagtatca agtaatactc
      361 tggctttctt atgctcatca ctaagcgtaa aaaaatcggc gatacgctgt tcaagcagtt
      421 gggtgtttga gaccacattc atacagtgat tttagctcat cgtgaaggcg cattaaataa
      481 ctatccgtca tgcctgagat tgcgtcggt
//
LOCUS       DAZSIO010000511          507 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZSIO010000511 DAZSIO010000000
VERSION     DAZSIO010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.518"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
BASE COUNT          166 a           76 c          101 g          164 t
ORIGIN      
        1 gaatgttgac acctgagtat ttcctgctaa aaatagccct gctttacgga tggttgtatt
       61 gcaataacac gaaagtagta agagattaaa ggtatctgtc agcgataact ctgcaagtga
      121 tgacattttg taaaccaaat aatgtaaatc agcaatgtat cttttcactt aattataaca
      181 aatgtggtat tcactaagaa tctgatacga gcttacatca cgactatttt agtctcatcg
      241 cgggatacag ggatgcaccg atggatctca gcacaccgta atgcaatacc tgaacaaata
      301 gaacagtaaa gtggttcagt attaatgaaa gctcttattt tccgttggga aaacttaaaa
      361 tagatgagaa ggctttttga tttcttatgt gatctcattt cgagtattga gaaatttgtg
      421 atttttaaat ttttcaataa gccctgtaag ggattggggt tttatgagaa ataaagagac
      481 gtattgtctt tgtttaaacg gtacgta
//
LOCUS       DAZSIO010000512          492 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZSIO010000512 DAZSIO010000000
VERSION     DAZSIO010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.519"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(199..>492)
                     /locus_tag="ACHX5T_004554"
     CDS             complement(199..>492)
                     /locus_tag="ACHX5T_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005137803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469401.1"
                     /translation="IHHSGVVNISSDNLQNILSKPREHTLNCLGWRFFVQRSVCVSWK
                     SDFAWTGIMLLFRGEELAFSSAFSPVTIRKIMKCPPPGRRTVEKRTRQQKHWG"
BASE COUNT          149 a          115 c           97 g          131 t
ORIGIN      
        1 ccccgacaga cctgtgctat cggaaagaaa aaaggccatc agcaaaactc tgatgacctg
       61 aatcaccttt accaatattg tatgaaaaaa cacgcacgac taattgacaa taattttcat
      121 ttccattaaa aaagatcgcg tagcattctt aattcatgag gaactttacc cgccagcaat
      181 ctgagtagcg aaagctgttc atccccaatg tttttgctgg cgggtccttt tttctacggt
      241 cctcctcccc ggaggggggc atttcattat ttttctaatc gttactggtg agaaggcgga
      301 agaaaaagcc agttcttcgc ccctaaataa cagcatgatt ccagtccatg caaaatctga
      361 tttccagctc acacataccg agcgctgtac aaaaaaccgc cagccaaggc agttaagagt
      421 gtgttcccgg ggtttgctta gaatattttg taagttgtcc gaagagatat ttacaacacc
      481 agaatgatga at
//
LOCUS       DAZSIO010000513          488 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZSIO010000513 DAZSIO010000000
VERSION     DAZSIO010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.520"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..488
                     /locus_tag="ACHX5T_004555"
                     /pseudo
     CDS             1..488
                     /locus_tag="ACHX5T_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          106 a          120 c          152 g          110 t
ORIGIN      
        1 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
       61 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
      121 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
      181 gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
      241 ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt
      301 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg taccgccatc
      361 gaaacgggca catacaggca gagtggccgt gaaagaaagc aatcagcgat ggtgctctga
      421 cgggttcgag ttctgctgtg ataacggaga gagactgcgt gtcacgttcg cgctggactg
      481 ctgtgatc
//
LOCUS       DAZSIO010000514          487 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZSIO010000514 DAZSIO010000000
VERSION     DAZSIO010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.521"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>487
                     /locus_tag="ACHX5T_004556"
     CDS             <1..>487
                     /locus_tag="ACHX5T_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HFR8469402.1"
                     /translation="LEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRLAA
                     DIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPVLT
                     SMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNKAL
                     KR"
BASE COUNT           97 a          163 c          131 g           96 t
ORIGIN      
        1 acttgagcac ccggcggtac tcgatcttct ccagcgatat ccctcaccag aaaaactcgc
       61 ttcgctgggt gagaagaagc tggcagccca gctctgcaaa cttgcgcctc gtctgggtaa
      121 acgccttgca gcagacatag ctcaggcact ggccgaacaa accgtcgtcg ttcccggcac
      181 gaatgccgct gccgtagtac tgccacgtct ggcactccag ctcatcacgc tgcgtaagca
      241 aagagacgag gtggcgcttg aggtagaaca gcgagttctt gctcaccctc tttacccggt
      301 cctgaccagt atgcccggag tcggtgtcag gaccgcagcc agactcctca ccgaggtcgc
      361 ctgccgcgcc ttcgcctctg ccgcacatct cgctgcttat gctggccttg cgccggtaac
      421 tcggcgatcc ggctcgtcaa tacgcggtga gcatccctcg cgacggggta ataaagctct
      481 caaacgg
//
LOCUS       DAZSIO010000515          485 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZSIO010000515 DAZSIO010000000
VERSION     DAZSIO010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.522"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..>485)
                     /locus_tag="ACHX5T_004557"
     CDS             complement(<1..>485)
                     /locus_tag="ACHX5T_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFR8469403.1"
                     /translation="KIECACNFLMDKDAQGYIDLSDFDLTNCHFKGDVISKVSFLSSN
                     LQHVTFECKEIENCNFTKATVDNVIFKCRRLHNVIFLKTSGECVDFSQNILDTVDFSQ
                     SQLGHSNFRECQIRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNLTAVMFDHVRMST
                     G"
BASE COUNT          170 a           91 c           73 g          151 t
ORIGIN      
        1 ttccctgtcg acattcgcac atgatcaaac ataacagctg tcaaattcga tttaataaat
       61 gatatttctt tggcagacag aaactctgct ctggtgaagt gcgaagcgta aagataacaa
      121 ttatcgaagt ttgaatttct aatctgacat tcgcgaaaat tactatgacc aagttgactc
      181 tgcgagaagt caactgtatc aagaatattt tggctaaaat caacacattc gccactcgtt
      241 ttgagaaaaa tcacattgtg taaacgtcga catttaaata tgacattatc aactgttgcc
      301 ttagtaaaat tgcaattctc aatttcttta cattcgaaag ttacatgttg tagatttgat
      361 gataaaaaag acaccttaga aataacgtca cctttaaaat gacaatttgt taaatcaaaa
      421 tcagacaggt cgatataccc ctgcgcatct ttatccatca gaaaattaca agcgcattct
      481 atttt
//
LOCUS       DAZSIO010000516          484 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZSIO010000516 DAZSIO010000000
VERSION     DAZSIO010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.523"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..484
                     /locus_tag="ACHX5T_004558"
                     /pseudo
     CDS             1..484
                     /locus_tag="ACHX5T_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          177 a           81 c           96 g          130 t
ORIGIN      
        1 gtcgagtaat aatgaatatg acctgtttaa aaatagagaa acctcactgt ggaaacgcaa
       61 agtaccagaa agcgcagcgc tggcaatgac gggaacagta tataaattat ttggtaatcg
      121 cttactcaac gcccgcaatc agttagataa aaatctcctc gaaatggcta tgcaaatggt
      181 ttatggcaaa gatatggtga caaagtccgg ggatatattg attgatatac aattaaataa
      241 ggatggcagt ctccagagta agcactacac acttgatgta ggtgaagtta taaacagtta
      301 taatattgac cctctttgtg aatgcttcaa gtattaatca aacttactta catgataaaa
      361 atcctggtga cgttattagc ctgtatggca ctttagataa cattcccctt gtagttaagg
      421 aatgcacggg taaaaatcat caaattaatg ttaaagccaa attgccacaa gaacgaagtg
      481 aaaa
//
LOCUS       DAZSIO010000517          478 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZSIO010000517 DAZSIO010000000
VERSION     DAZSIO010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.524"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..139
                     /locus_tag="ACHX5T_004559"
     CDS             <1..139
                     /locus_tag="ACHX5T_004559"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8469404.1"
                     /translation="AGLKPDTMRNVFRCHIPRYEAIIARYLDMEPAQIWPSRYMEKTS
                     C"
     gene            172..478
                     /locus_tag="ACHX5T_004560"
                     /pseudo
     CDS             172..478
                     /locus_tag="ACHX5T_004560"
                     /inference="COORDINATES: protein motif:HMM:NF014380.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT          139 a          107 c          118 g          114 t
ORIGIN      
        1 ggcaggctta aagcctgaca ctatgcgtaa cgtatttcgc tgccatatac cacgatacga
       61 agcaatcatt gctagatatt tggatatgga accagcccag atttggcctt ctcggtatat
      121 ggagaaaaca tcatgttaac ccattgggta acagcgcaag aatgcgtagg aatgtcatgc
      181 tttcctaaac atgaggtgag tattcgccgc aatcttgaaa agcttgcagc caatcatcca
      241 gaactacgtc gcaagcgtac gggttcaaag gctttggaat ttcatatatc agttctacca
      301 gcggcagcac gtgctgagtt gttattaaaa tctgagcaaa tagaaacaag ctaggggtat
      361 tttgaaatcg cccgccccac gctggaagcg cacgattacg atcgtgaggc actgtggagc
      421 aaatgggata aagccagcga ttcccagcgc agacttgctg aaaaatggtt gcctgcgg
//
LOCUS       DAZSIO010000518          472 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZSIO010000518 DAZSIO010000000
VERSION     DAZSIO010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.525"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            3..>472
                     /locus_tag="ACHX5T_004561"
     CDS             3..>472
                     /locus_tag="ACHX5T_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HFR8469405.1"
                     /translation="MDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKL"
BASE COUNT          101 a          130 c          144 g           97 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ct
//
LOCUS       DAZSIO010000519          471 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZSIO010000519 DAZSIO010000000
VERSION     DAZSIO010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.526"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..361
                     /locus_tag="ACHX5T_004562"
     CDS             <1..361
                     /locus_tag="ACHX5T_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8469406.1"
                     /translation="DHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVC
                     GEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVE
                     GLKKNARDISLLRYMPA"
BASE COUNT          128 a           99 c          161 g           83 t
ORIGIN      
        1 ggaccacaaa acgggagaaa cggcgacgga aacgctgaca cagcgtgagc tggtcgcgag
       61 gctgaaacag cacatcccgg agaagttttt taagatggtg aggtacttcg ggttccttgc
      121 caaccgggtg tgtggagaga agctgccgca ggtgtaccgt gctctgggga tggataaacc
      181 ggaaccagtg gcgaaagtgt gctatgcaca aatggtgaaa cagttcctga gtcgtgaccc
      241 gttcgaatgc gtgctgtgtg gcggccggat ggtataccgc cgggccatcg cgggactgaa
      301 tgtggagggg ctgaagaaaa acgcgcggga tatcagtctg ctgaggtata tgccggcctg
      361 acacaggcta agtgtgcccg ggaatcggtg caagtataaa aaacagcctg ttgcggtata
      421 agaatcgacc atgcagagag agaaaacacc ggagtggagg gaaaagcaga a
//
LOCUS       DAZSIO010000520          469 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZSIO010000520 DAZSIO010000000
VERSION     DAZSIO010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.527"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..>469)
                     /gene="wzx"
                     /locus_tag="ACHX5T_004563"
     CDS             complement(<1..>469)
                     /gene="wzx"
                     /locus_tag="ACHX5T_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HFR8469407.1"
                     /translation="AICIPLFILNQLWSAILEGDEKFGIVNIQKSISSSCIAGIPAIF
                     VFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLFFFGGWMTVS
                     NIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVFPRLSN"
BASE COUNT          171 a          100 c           69 g          129 t
ORIGIN      
        1 tgttacttaa ccttggaaac actgccctcc cgattgctgc aggtattatt cctaatttta
       61 gaataacctc cgctggtgca gaataaaatg caactttatc tgcccccata atatttgaca
      121 ctataaatcg atcaaaatac accatgaccg gacttatgat attacttact gtcatccaac
      181 cgccaaagaa aaagagtcgt ttaaaggttt tataacaaaa atgaaccccc gaaattttaa
      241 tatcattcct gacataatag gcagagacta aaatcgaaat aactcttgca aatattaaac
      301 cagcaaccgc tgccgacaat gtagcactat aaaaaacaaa tatggccgga attcccgcga
      361 tgcaagagct tgatatagat ttttgaatat ttacaatgcc aaatttttca tccccctcaa
      421 gaatggctga ccataattga tttagaataa ataatggaat gcaaatagc
//
LOCUS       DAZSIO010000521          465 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZSIO010000521 DAZSIO010000000
VERSION     DAZSIO010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.528"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>465
                     /locus_tag="ACHX5T_004564"
     CDS             <1..>465
                     /locus_tag="ACHX5T_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR8469408.1"
                     /translation="SGSTLISRDNKLVENFIDTLENWRDQKQIISVICKDGIKIDDSI
                     ITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDVSAATGRTSTTNDGGATSKKNTG
                     NTTTSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSVKNGESTFTASA"
BASE COUNT          147 a           96 c          102 g          120 t
ORIGIN      
        1 aatccggtag tacccttatc tcacgagata ataagctggt ggagaacttc atcgacactc
       61 ttgaaaactg gcgtgaccag aagcagatta tttcagttat ctgtaaagat ggtattaaga
      121 ttgacgactc cattattaca agctttaaag cctctaaaga tgttggaatt tcaaacggac
      181 tgagaatcca gctaactttt caggaaatta acttcaaagc tattgtaggg caaactgatg
      241 tttcagcagc cactggacgg acttctacca cgaatgatgg cggtgctacc agtaaaaaga
      301 acactggcaa cactacgaca agtcttggaa gccctatgct gacctgtaaa gagcttttta
      361 gctattcggc aagtgagtta tcagatgaag cgttaaaagc ccgtgtaacg tgctctaaga
      421 gcgttagcgt taaaaatggt gagagcacct tcacagcctc agcta
//
LOCUS       DAZSIO010000522          465 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZSIO010000522 DAZSIO010000000
VERSION     DAZSIO010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.529"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>465
                     /gene="gtrII"
                     /locus_tag="ACHX5T_004565"
     CDS             <1..>465
                     /gene="gtrII"
                     /locus_tag="ACHX5T_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HFR8469409.1"
                     /translation="RSLKLESYELLIGMLVFITFPQISYQLEFLNQADTVGIAFLLAA
                     ISAIIFHSQKNRIVIFSGIVLSILSMAIYQTFVTYIIAFVIGLQINSIIRNEKNIRES
                     FYSSCLSLSLIALSTLIYLLLTKAIKHYFSLESNEYISNYIQNASDIKWLVKS"
BASE COUNT          146 a           83 c           67 g          169 t
ORIGIN      
        1 cagatcgcta aagctagaat cttatgaatt attgataggt atgttagtat ttattacctt
       61 ccctcagatc tcctatcaat tagagtttct taaccaagct gatactgtgg gaattgcttt
      121 tctactggca gcgatatcag caattatttt tcactcgcaa aaaaatagga ttgtgatatt
      181 ttctggtata gtactgtcca ttctttcaat ggcaatctac caaacattcg taacatatat
      241 tattgcattc gtcattgggt tgcagataaa ttcgataata cgaaatgaga aaaatattcg
      301 tgaatctttt tatagttcat gtttatctct atccctcata gctttatcta ccttaattta
      361 cctgctatta accaaagcta tcaagcatta tttttcgctt gaatcgaacg agtacatctc
      421 aaattatata caaaatgcaa gcgatattaa atggcttgtt aaatc
//
LOCUS       DAZSIO010000523          459 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZSIO010000523 DAZSIO010000000
VERSION     DAZSIO010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.530"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..>459)
                     /locus_tag="ACHX5T_004566"
     CDS             complement(<1..>459)
                     /locus_tag="ACHX5T_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HFR8469410.1"
                     /translation="LSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLLM
                     FNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVRE
                     KLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEHDAGFA"
BASE COUNT          106 a          117 c           90 g          146 t
ORIGIN      
        1 gggcaaatcc agcatcatgt tcgaacacca ctccaacatc tgtttgaaca cctgttaaat
       61 ttctctcttg ttttgtttga acatctgctt cctttctgct tcttctggcc tgaacagatg
      121 cttttcctgc ggctgatttt ttggttaatt tttctctgac agatgccaga tcttcctcaa
      181 tccgaagatg catccattcg tcgccgttat cacaaaaaaa ctcccgcaag gatggttcaa
      241 catcaaccca tcgctcgtta gtcagacggg caatttttgc caacctgttt ttgggtattg
      301 gctttcctgt ttgccagtaa ttgaacatca gcaacaaata cgcgccgtgc tcctctgctg
      361 acaaatgcat ggtgtcagcc aggtaatcag ctatgtacag ttgcatgtat ggtaatgcgg
      421 ccataattgc cccgtatgat gctacccggt tgcttagaa
//
LOCUS       DAZSIO010000524          457 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZSIO010000524 DAZSIO010000000
VERSION     DAZSIO010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.531"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>457
                     /gene="spa32"
                     /locus_tag="ACHX5T_004567"
     CDS             <1..>457
                     /gene="spa32"
                     /locus_tag="ACHX5T_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HFR8469411.1"
                     /translation="KKKRKVEIPEYSLIASNYFTIDKHFEHKHDKGEIYSGIKNAFEL
                     RNERATYSDIPESMAIKENILIPDQDIKAREKINIGDMRGIFSYNKSGNADKNFERSH
                     TSSVNPDNLLESDNRNGQIGLKNHSLSIDKNIADIISLLNGSVAKSFELP"
BASE COUNT          174 a           63 c           86 g          134 t
ORIGIN      
        1 gaagaagaag aggaaggttg aaattcctga gtactcttta attgcatcta actattttac
       61 tattgataag cactttgaac ataagcatga taaaggagaa atttatagtg gcattaaaaa
      121 tgcgttcgaa cttagaaacg aacgagcgac atattctgat attccagaat caatggccat
      181 taaagaaaat attttgatac cagatcaaga tatcaaagca agggaaaaaa taaatatcgg
      241 cgatatgagg gggatctttt catataataa gagtggaaat gcagacaaga acttcgaaag
      301 aagtcatact tcttctgtaa accctgataa tctgctagaa tctgataata gaaatggtca
      361 aattggttta aaaaatcata gcttgtctat tgataagaat attgctgaca tcatttcttt
      421 actaaatgga agtgttgcta aatcatttga gctgcct
//
LOCUS       DAZSIO010000525          454 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZSIO010000525 DAZSIO010000000
VERSION     DAZSIO010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.532"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
BASE COUNT          149 a           75 c           92 g          138 t
ORIGIN      
        1 ttgttaaaag aaataagtag gagtttttat gaagttcatt ccatgaaatg aacttcagca
       61 cttttttatt ataaaaggac aaacaaaatt ggctgtaaaa aaacgatact gtttcagtta
      121 actgtgaata gcttgcttgc accgtattgt tcttgttttt cggttagtta aacatcgtac
      181 ttcatatttg aacgttctgc cggaatgcat tatcaataga ggtaaagtcg caaccccaaa
      241 tcgtaaagga aaccgtagca cgtcgtatgc aagaacgtgc cacggctggc tgatggatgt
      301 tcgatagcgc gagtttgaat gaaaatcagc cggagatgat tttacataat tgctacggaa
      361 ttattcaata caggaattgc ttgcgtctgc atggattgac ctgaaatatt cccgaaaatt
      421 tctctaaaaa actcgaaaaa aatggtaact ggtt
//
LOCUS       DAZSIO010000526          444 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZSIO010000526 DAZSIO010000000
VERSION     DAZSIO010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.533"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..414
                     /locus_tag="ACHX5T_004568"
     CDS             <1..414
                     /locus_tag="ACHX5T_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8469412.1"
                     /translation="HRALSEHKTLSVSWSHYLNGKVIAPACTLVAATKDSVVTLPNVS
                     ATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADGVQPTAFANQATTDAAT
                     NVALQMYLPDGSTSVTPGTETSNIQLADSVMTPTY"
BASE COUNT          125 a          100 c           98 g          121 t
ORIGIN      
        1 catcgggcat tatctgaaca taaaacacta tcagtaagtt ggagtcatta ccttaatggt
       61 aaagttattg ctccagcttg taccctggta gctgcgacga aagattcagt agtcacattg
      121 ccaaatgtca gcgccaccaa gttgcaaact aatggagcag tctctggcgt taaaactgat
      181 gtaccaattg ccttagaagg ctgtgatgtt actgtaacta aaaatgcgac gtttaccttt
      241 agtggtacgg ctgacggcgt gcaacctaca gcatttgcca accaggctac caccgatgca
      301 gcgacgaatg ttgctctaca aatgtatctg ccagatggtt ctacatcggt aactcccggt
      361 accgaaacgt cgaatattca gttagcggat tcggtaatga ctccaactta ctgatagtgt
      421 tttatgttca gataatgccc gatg
//
LOCUS       DAZSIO010000527          443 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZSIO010000527 DAZSIO010000000
VERSION     DAZSIO010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.534"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..>443)
                     /gene="ipaH7.8"
                     /locus_tag="ACHX5T_004569"
     CDS             complement(<1..>443)
                     /gene="ipaH7.8"
                     /locus_tag="ACHX5T_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HFR8469413.1"
                     /translation="NNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEERGIAF
                     NRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKELNADN
                     NRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYN"
BASE COUNT          137 a           63 c           96 g          147 t
ORIGIN      
        1 gttatatagc ctgttatttt caacaaataa tgatgttaaa tggtttggca aaacaggaag
       61 gttttccagt tgattagaac gaacacttaa agttgttaat gactcaggaa gttctggtat
      121 cacagaaagc ctgttattat cagcattaag ttctttaaga aatgcaggca gctttggcaa
      181 tgatgttaat ttattatttt ctacaatcaa agcagaaata tgctttggta attcgggaag
      241 agacgtcaaa tttaggtctg ataaatttaa tactgcttct tgattctgaa agcactggga
      301 gagtctgtta aaagcaatgc ctcgctcttc cccgggagaa gagttctttt cccattcagt
      361 caggattctc agataatgtt cattactggt tgagtttgag ttaatagagg gggagcaaga
      421 aactgatgag tgtgtattat tta
//
LOCUS       DAZSIO010000528          443 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZSIO010000528 DAZSIO010000000
VERSION     DAZSIO010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.535"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            213..>443
                     /locus_tag="ACHX5T_004570"
     CDS             213..>443
                     /locus_tag="ACHX5T_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFR8469414.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDSRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          141 a          105 c           61 g          136 t
ORIGIN      
        1 tatcgaacct cgcttaccaa cgcgttgtca gtcatctccc ccctctctgg tgcatcgtta
       61 tatccgtgat gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt
      121 cagaaaacaa acttattttt attaaaatca attctttttc tttgaacatt caactgcgat
      181 atttgtaaaa acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat
      241 tccccgcata tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc
      301 aatacgcacg cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatagcagac
      361 gtactgttga gatccacaaa ctagatctct ctgactggca agcattcaat aagttagcaa
      421 cgaagtgtaa tgcttacgat gga
//
LOCUS       DAZSIO010000529          438 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZSIO010000529 DAZSIO010000000
VERSION     DAZSIO010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.536"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            95..>438
                     /locus_tag="ACHX5T_004571"
     CDS             95..>438
                     /locus_tag="ACHX5T_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469415.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
BASE COUNT          143 a           67 c           95 g          133 t
ORIGIN      
        1 tttataatga gcgatttttg atatctcgcc gagaaaatta tcagcattct ctcgtttcag
       61 tgtcgtgaat atactgttaa cggaggtgaa aagaatgctg ggggttatac aaaacctatg
      121 ttgttgtcgt ccggattcac cgcttgctga tagtaagcag cgtaaccaga agttaccgca
      181 acaagacttt gaggatttta agaacgacgt tattaatgtc attaacaaca atgaagatat
      241 ttattttctt agagagagta atttactgaa tgcgtttatt cggacgtgca aaaaaagaga
      301 tgataacagt tacggaaata tgatatttac gctttatttt gataacgata tctatgggaa
      361 cagaattatc aaagcatcgc tgaacggcgg agagttgcag ttattaagca cagggtgtag
      421 caatgataac attctcca
//
LOCUS       DAZSIO010000530          434 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZSIO010000530 DAZSIO010000000
VERSION     DAZSIO010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.537"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            35..434
                     /locus_tag="ACHX5T_004572"
                     /pseudo
     CDS             35..434
                     /locus_tag="ACHX5T_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAZSIO010000531          434 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZSIO010000531 DAZSIO010000000
VERSION     DAZSIO010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.538"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>434
                     /locus_tag="ACHX5T_004573"
     CDS             <1..>434
                     /locus_tag="ACHX5T_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR8469416.1"
                     /translation="SAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWVSE
                     MADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGSSL
                     PAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYET"
BASE COUNT          112 a          103 c          128 g           91 t
ORIGIN      
        1 ccagtgcagg gttacttgca cggatcctgg tcagcaaata tatggaacat atccctttat
       61 atcgccagtc agaaatatac gcgcgacagg gcgtggagct gagccgtaat accatggtgc
      121 gctgggtatc agaaatggca gacaaactcc gtcctctgta tatagcgctg aatgactatg
      181 ttctggaggc aggaaaggtg cacgcagatg acactccggt gaaagtactg gccccgggga
      241 acggaaagac gaaaacgggt cgtctgtggg tatacgtcag ggatgatcgt aatgcgggtt
      301 catccctgcc ggcagccgtc tggttcgcgt attcggcaga tcgcaaagga gaacatccgc
      361 agctccacct ggcaaagtat cagggcgtac tgcaggctga tgcctatgca ggttataacg
      421 tactgtacga aacg
//
LOCUS       DAZSIO010000532          434 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZSIO010000532 DAZSIO010000000
VERSION     DAZSIO010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.539"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..>434)
                     /gene="rfbC"
                     /locus_tag="ACHX5T_004574"
     CDS             complement(<1..>434)
                     /gene="rfbC"
                     /locus_tag="ACHX5T_004574"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFR8469417.1"
                     /translation="GFFMESFNQKVFEEAVGRKVEFVQDNHSKSTKGVLRGLHYQLEP
                     YAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVNLSAENKRQLWIPEGFAHGFCVLS
                     DEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQNPLLSDK"
BASE COUNT          129 a          110 c           65 g          130 t
ORIGIN      
        1 ctttatcaga aagcaatgga ttttgaaccg gccattctat atttatactt ttgtcgctcc
       61 ataaaatccc acgctcttgc atttttgaat agaaattatt tgttttgtag acaaattctg
      121 cttcatcact aagaacgcaa aacccatgag cgaatccttc cggtatccat aattgacgct
      181 tattctccgc cgacaaattt acaccaaccc acttgccaaa tgttggtgat gatttccgaa
      241 tatcaacagc tacgtcaaaa acttcaccga caacacagcg taccagtttt ccttgagcat
      301 aaggttccag ctgatagtgc agtccgcgta acacaccctt agttgattta gaatggttat
      361 cctgaacaaa ctcaaccttt cgtcctacag cctcttcgaa aactttctga ttgaagcttt
      421 ccataaaaaa gccc
//
LOCUS       DAZSIO010000533          432 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZSIO010000533 DAZSIO010000000
VERSION     DAZSIO010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.540"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>432
                     /gene="pgrR"
                     /locus_tag="ACHX5T_004575"
     CDS             <1..>432
                     /gene="pgrR"
                     /locus_tag="ACHX5T_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR8469418.1"
                     /translation="KREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEER
                     LGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVEH
                     AAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGV"
BASE COUNT          103 a          108 c          109 g          112 t
ORIGIN      
        1 aagcgtgaag aaatcgctga tctgatggcg tttgtcgtcg ttgcagagga gcgtagcttc
       61 actcgcgcag cagcccgcct gagcatggtg cagtcagctt taagccagat agtgcgccgt
      121 atagaagaac gattgggatt gcggcttctg acgcgaacca cgcgcagcgt tgtacccact
      181 gaagcgggcg agcatctttt gtctgttctt ggccctatat tgcatgacat agattctgcc
      241 ctgacatccc tgagcgatct gcagaaccgc ccatccggga caatacgtat tactactgta
      301 gaacatgcag caaaaacgat attgttacca gccatgcgca cattcctgaa atcgcatcct
      361 gaaattgata ttcagcttac cattgattat ggtttgaccg atgtcgtttc tgaacgtttt
      421 gatgcaggcg tc
//
LOCUS       DAZSIO010000534          428 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZSIO010000534 DAZSIO010000000
VERSION     DAZSIO010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.541"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(109..312)
                     /gene="safA"
                     /locus_tag="ACHX5T_004576"
     CDS             complement(109..312)
                     /gene="safA"
                     /locus_tag="ACHX5T_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR8469419.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
BASE COUNT          144 a           69 c           72 g          143 t
ORIGIN      
        1 aaagcatgat gaataaatat aacagaacaa acgagcgaca ttttatctcc ttaaaacaat
       61 aaagttttat cgataccttt taaacatttc acatttataa tttgctgttt atttccagtc
      121 ttgcaaacta ttgataatga aatgtgtaag atctcttgca atgcgaccca tttctgaaat
      181 actcagctga tcaattgccg aaaaaatggc tatcaacgta agtgtcagta ataagacaac
      241 cacaattaca gacatgattt ctttatagtt atctctttgc gtgattttgt ttttcactgt
      301 ggtcgcatgc atttcaaata tgtttattta gcggataacg ttaaaagtcg ttaatcagta
      361 tgcttaatat aacaggctgg agaaaagaag aaataggact ggtgcttcag tactgagcgg
      421 agtttctt
//
LOCUS       DAZSIO010000535          414 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZSIO010000535 DAZSIO010000000
VERSION     DAZSIO010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.542"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(180..389)
                     /gene="fumD"
                     /locus_tag="ACHX5T_004577"
     CDS             complement(180..389)
                     /gene="fumD"
                     /locus_tag="ACHX5T_004577"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR8469420.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          117 a           98 c           67 g          132 t
ORIGIN      
        1 taacttaagc gacaatcatt tgtgacgttc attgcagaaa ataaaaataa cattatcgct
       61 ataaattaaa atacataccg aaattcgtat aatttcagca gttgttattt agaatgatta
      121 actggaccgc aactgaagca agaatatcag gatgaagctt accgcctcat cctgaatggt
      181 cattttacca gtgcgttaat gacggtttcc atcgcctgct tttccagctc actgcgctga
      241 atgcgtttat tggctaatgc tgtacgtaat acgcctgcga taacaatatg ctttggttcc
      301 tgccccagat cgcgcatttc cagcacaact ttaccgacaa ctctgcacat ttctcggtac
      361 agttcatcct cttttgtccg atttcccatt ctttatacct atttatcatg ccaa
//
LOCUS       DAZSIO010000536          411 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZSIO010000536 DAZSIO010000000
VERSION     DAZSIO010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.543"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>411
                     /gene="ospC2"
                     /locus_tag="ACHX5T_004578"
     CDS             <1..>411
                     /gene="ospC2"
                     /locus_tag="ACHX5T_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HFR8469421.1"
                     /translation="AVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSSSGISE
                     KHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSLEHLDK
                     VSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNF"
BASE COUNT          144 a           79 c           62 g          126 t
ORIGIN      
        1 aagcagtaaa tcatattaat gttcagaaca atattgatct tgttgatgga aaaataaatc
       61 cgaacaaaga tacaaaagca ttacaaaaaa acatatcatg cgtaacaaac tcatcctctt
      121 ctggcataag tgaaaaacat ttagatcact gtgctgatac tgtgaaaagc tttttaagga
      181 agtctatagc agcgcaaagt tatagtaaaa tgttctctca agggaccagt ttcaaatcat
      241 taaatctttc tattgaagcg ccatctggag cacggtcatc atttcggtca cttgagcacc
      301 tcgataaggt gtccagacac tatctttctg aaataataca aaaaactcat cctctttctt
      361 cggatgaacg tcacttgctc tctattatta ttaattctga ttttaacttc a
//
LOCUS       DAZSIO010000537          410 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZSIO010000537 DAZSIO010000000
VERSION     DAZSIO010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.544"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            156..341
                     /locus_tag="ACHX5T_004579"
                     /pseudo
     CDS             156..341
                     /locus_tag="ACHX5T_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          119 a           97 c          108 g           86 t
ORIGIN      
        1 atacggtggt cggtttacgc ttaccgcagg acaggtggta actgaaaaat agtttgctga
       61 ggcgactgga aacagaaggg caagatcgat tccgacagca gtgatggtca gctacctcat
      121 cgataacttt gcaacagtgc ccttcttgaa gcatcatgct tcatgaagaa aaactggcac
      181 gtcatcaacg taaacaggcg atgtataccc gaatggcagc cttcccggcg gtgaaaacgt
      241 tcgaagagta tgacttcacg ttcgccaccg gagcaccgca gaagcagctc cagtcgttac
      301 gctcactcag cttcatagag cgcaatgaaa acatagtttt acaggggaca tctgacatca
      361 caaatccccg ggtggggatt tgtgtataag agacagccca tggaggaagg
//
LOCUS       DAZSIO010000538          410 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZSIO010000538 DAZSIO010000000
VERSION     DAZSIO010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.545"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
BASE COUNT          148 a           74 c           72 g          116 t
ORIGIN      
        1 gacaaagata aatgaagccg gaattgatga aaaacaaagc caacggtatt ctgatttctg
       61 aagttaattt cagactcgtt taagctccat tttcaaaaaa aatatatcag ggtttgttac
      121 taataacttc tgaaaacaga aaaaaataga gcagtaattc tatttcagag aggagtaact
      181 ccatctatcc tcgatacgta ctgaacgtta tcgtgatata ttcatcatgc tttcttcacc
      241 attattcgag tatagggaga gacgcaatga ccttaaatac ctttttatgg ctgaattacc
      301 attctcagga ataacagcag actccttccc atagacggct tagggggaaa gcaatacccg
      361 gagaaagggt acaaagcaaa ctgttttaaa attaataagc catattctat
//
LOCUS       DAZSIO010000539          408 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZSIO010000539 DAZSIO010000000
VERSION     DAZSIO010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.546"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..408
                     /locus_tag="ACHX5T_004580"
                     /pseudo
     CDS             1..408
                     /locus_tag="ACHX5T_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          100 a          111 c          121 g           76 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaagg
//
LOCUS       DAZSIO010000540          407 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZSIO010000540 DAZSIO010000000
VERSION     DAZSIO010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.547"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..269
                     /locus_tag="ACHX5T_004581"
     CDS             <1..269
                     /locus_tag="ACHX5T_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR8469422.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..>407
                     /gene="tnpB"
                     /locus_tag="ACHX5T_004582"
     CDS             266..>407
                     /gene="tnpB"
                     /locus_tag="ACHX5T_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR8469423.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFR"
BASE COUNT           99 a          109 c          114 g           85 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggg
//
LOCUS       DAZSIO010000541          404 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZSIO010000541 DAZSIO010000000
VERSION     DAZSIO010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.548"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>404
                     /locus_tag="ACHX5T_004583"
     CDS             <1..>404
                     /locus_tag="ACHX5T_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HFR8469424.1"
                     /translation="HVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVITL
                     VEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDEV
                     METLAHALRELRNAKKNFVLLVSVDIRRFVKRL"
BASE COUNT          128 a           50 c           93 g          133 t
ORIGIN      
        1 catgtgacta tacagagaat ttctgaggta ttacaaagat tgcttggaga aaatatttct
       61 gttcgcaatt taaaacttat tatggagtct ttggcgcttt gggctcccag agaaaaagat
      121 gtcataacat tagttgaaca tgtccgtgca tcactttcta ggtatatttg tagtaaaata
      181 gctgtttctg gtgagattaa agttgtgatg ctttccggat atattgagga tgcaataaga
      241 aaagggataa ggcaaacctc tggtggctct ttcttgaata tggatataga ggtttcggat
      301 gaggtaatgg aaactttagc acatgctttg agagaattga gaaatgcaaa aaaaaatttc
      361 gttcttttgg tatcagtaga tatacgtagg tttgttaaaa gact
//
LOCUS       DAZSIO010000542          402 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZSIO010000542 DAZSIO010000000
VERSION     DAZSIO010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.549"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..>402)
                     /locus_tag="ACHX5T_004584"
     CDS             complement(<1..>402)
                     /locus_tag="ACHX5T_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8469425.1"
                     /translation="NNASYQLNNALPNKETISSNFCERLEQWGNKSVNNGEERAIAVE
                     RIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLADNASLVNINLSV
                     NKINTITFPKEPKLENIYINHNNLESLDLKN"
BASE COUNT          114 a           62 c           74 g          152 t
ORIGIN      
        1 tgatttttta aatctaaact ttccagattg ttatgattaa tataaatgtt ttccagtttt
       61 ggttcttttg gaaaggttat cgtgttaatt ttattaacgc tgaggttgat attaacgaga
      121 ctggcattgt cagctaagtc aagacaagtt agacaattgt tttccagatt taacgtatta
      181 actgtcgagg ggataggcgg taattcactc aaatcaagat atgacaaatc aagagatgcc
      241 atgttcgaat tgtaagcttc tttaattcgc tccacggcaa ttgctctttc ttcaccatta
      301 ttaaccgatt tattacccca ttgttctagt cgttcacaaa aattgctgga gatggtttct
      361 ttgttaggta atgcgttatt aagttgataa ctggcattat tt
//
LOCUS       DAZSIO010000543          398 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZSIO010000543 DAZSIO010000000
VERSION     DAZSIO010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.550"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..116)
                     /locus_tag="ACHX5T_004585"
     CDS             complement(<1..116)
                     /locus_tag="ACHX5T_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469426.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVN"
     gene            134..325
                     /locus_tag="ACHX5T_004586"
     CDS             134..325
                     /locus_tag="ACHX5T_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR8469427.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          105 a          103 c           73 g          117 t
ORIGIN      
        1 aaattaactt ccataacatt aaaatatgta tccactgacg cttttttaca taacgaagaa
       61 ttgaccattt tgtcctgttg tgccttaatg taagtaccgt ccacagcgtg ggacatactt
      121 caaggaacct tttgtgagtc aggcaactag tatgcgaaaa cgacaccgat ttaacagtcg
      181 catgacccgt atcgtactgc tcatcagctt tatcttcttc tttggccgtt ttatctactc
      241 gtccgtcggt gcctggcagc accatcagag caaaaaagaa gctcagcaat ccacactctc
      301 cgtcgaatca ccggtacaac gttagcggtt accttctcca ctttcacaga acataacggc
      361 acttcgctgt cggatgcttt tgctctttgg gattatca
//
LOCUS       DAZSIO010000544          397 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZSIO010000544 DAZSIO010000000
VERSION     DAZSIO010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.551"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>397
                     /locus_tag="ACHX5T_004587"
     CDS             <1..>397
                     /locus_tag="ACHX5T_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFR8469428.1"
                     /translation="NGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQIT
                     TLEIRKNLLTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPEN
                     LTHLRVHNNRLHILPLLPPELKLLVVSGNR"
BASE COUNT          145 a           84 c           63 g          105 t
ORIGIN      
        1 aacggtaccc caggcgagca acgcaatatc gccttcaata ggctgaaaat atgtttacaa
       61 aatcaagagg cagaattaaa tttatctgag ttagatttaa aaacattacc agatttaccg
      121 cctcagataa caacactgga aataagaaaa aacctattaa cacatctccc tgatttacca
      181 ccaatgctta aggtaataca tgctcaattt aatcaactgg aaagcttacc tgccttaccc
      241 gagacgttag aagagcttaa tgcgggtgat aacaagataa aagaattacc atttcttcct
      301 gaaaatctaa ctcatttacg ggttcataat aaccgattgc atattctgcc actattgcca
      361 ccggaactaa aattactggt agtttctgga aacagat
//
LOCUS       DAZSIO010000545          396 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZSIO010000545 DAZSIO010000000
VERSION     DAZSIO010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.552"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..212)
                     /locus_tag="ACHX5T_004588"
     CDS             complement(<1..212)
                     /locus_tag="ACHX5T_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469429.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETPKSIMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          117 a           76 c           60 g          143 t
ORIGIN      
        1 taacaggatg ctctgactcg caattcatcc agcagtttat catttcccat aatgtcactt
       61 accctcatcg gtaaaaaaat tgcctttcga ccattacgat acattattga ttttggggtt
      121 tcagggaagt aatccatttc gactataact gacaggtcat cacgacgtat gactgcgtat
      181 ttgctactaa atagtttctt tattttttcc acgatgcccc caggtttata agtacaaacg
      241 gttatatcca catagagaca aaaatattaa tctgataaat atttatttca cgccgtatat
      301 ttgattgttt aatgtgcaag tacaatgact tttatttttt gttgtgtata taatcaaata
      361 tatggttatt tttcaccctg cgtattcagc acgcaa
//
LOCUS       DAZSIO010000546          391 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZSIO010000546 DAZSIO010000000
VERSION     DAZSIO010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.553"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            19..360
                     /locus_tag="ACHX5T_004589"
                     /pseudo
     CDS             19..360
                     /locus_tag="ACHX5T_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          129 a           61 c           81 g          120 t
ORIGIN      
        1 caaaataaag ctaataacat ccaattaaga tatgatgata agcagtttca tacaacggtt
       61 atcaaagatg ttctattatg gattgaacat aatttagatc agtctttact gcttgatgat
      121 gtggcgaata aagcgggtta taccaagtgg tattttcagc ggctgttcaa aaaagtaaca
      181 ggggtcacac tggctagcta tattcgtgct cgtcgtttga cgaaagcggc tgttgagttg
      241 aggttgacga aaaaaactat ccttgagatc gcattaaaat atcaatttga ttcccaacaa
      301 tcttttacac gtcgatttaa gtacattttt aaggttacac caagttatta tcggcgtaat
      361 aaattatggg aattggaggc aatgcactga g
//
LOCUS       DAZSIO010000547          391 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZSIO010000547 DAZSIO010000000
VERSION     DAZSIO010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.554"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
BASE COUNT           97 a           85 c           79 g          130 t
ORIGIN      
        1 gattttaata tgttgtatgg ttttcactct gaaatcccct gaatttaatc acgtttcctt
       61 tactgaaggc aattttaacc gtagtatcaa ttattaaccc ttgggcttga tatccctctc
      121 ctcttctggg cacgggggat gtcagattac ttattacaga aaccaaaaga attgtaagcg
      181 tcatctcagt gtcgtctatt tcaatgtctg atgattggcg atgatagagt cctcttataa
      241 cgttaatgga ctctatcaat gtcagattct cttcgccccc gcaggattcg tgcctcttac
      301 tctatgactc tatggagttt aagcttgatc ttattgaaaa atcttaccag ctaggggcct
      361 gcgtggccca actggccaga gagtacggga t
//
LOCUS       DAZSIO010000548          390 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZSIO010000548 DAZSIO010000000
VERSION     DAZSIO010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.555"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(42..>390)
                     /locus_tag="ACHX5T_004590"
     CDS             complement(42..>390)
                     /locus_tag="ACHX5T_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8469430.1"
                     /translation="QIKPFLEKAMTMYNDVLFPGEDINKPISIAAANRFVNRIRGGMD
                     LGYWRTHDFRRTLVTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELH
                     ADKLFWHIRSISD"
BASE COUNT           92 a           91 c           87 g          120 t
ORIGIN      
        1 ccttcgtgaa gctgttgaag ggtggattct taacggtggc gttaatcaga aatgctcctg
       61 atgtgccaga acaatttatc agcgtgaagc tcatacgctt tgcgctgagc ctctatccag
      121 tcgtgtttat tgtatacgga cattatcccg ccaagttcat gaccgagcat tcgctcagta
      181 acatgaggct cgacattcat ctcggacagc cgtgtaacaa gcgttcttct gaaatcatgt
      241 gttcgccagt aacctagatc catccctccc cttattctat ttacgaatcg gttggctgca
      301 gcgatgctga tcggtttgtt tatgtcttct ccagggaaaa gaacatcatt gtacattgtc
      361 atggcctttt cgagaaaagg cttaatttgt
//
LOCUS       DAZSIO010000549          383 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZSIO010000549 DAZSIO010000000
VERSION     DAZSIO010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.556"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>383
                     /gene="mxiC"
                     /locus_tag="ACHX5T_004591"
     CDS             <1..>383
                     /gene="mxiC"
                     /locus_tag="ACHX5T_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFR8469431.1"
                     /translation="KNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKYIDSS
                     DEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLPLDRD
                     FIDRLKRYFKDPSDQVLALRELLNE"
BASE COUNT          137 a           50 c           83 g          113 t
ORIGIN      
        1 ttaaaaatac aggagttttt agctctgcat tcattgataa gttgaatgca atgacaaatt
       61 cagatgatgg agatgagact gctgatgcag agcttgattc tggcttggct aatagcaagt
      121 atattgactc atctgatgag atggcttccg ctctttcgtc atttataaac agaagagacc
      181 ttgagaaact gaaaggaaca aatagtgata gtcaggaacg tattttagat ggggaagaag
      241 atgaaattaa tcacaagatt tttgatttaa agagaacgtt aaaagataac cttcctctag
      301 atcgggattt tatagacaga ctaaagagat attttaaaga tccaagtgat caagtcttag
      361 cattaaggga acttttgaat gaa
//
LOCUS       DAZSIO010000550          380 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZSIO010000550 DAZSIO010000000
VERSION     DAZSIO010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.557"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(1..248)
                     /locus_tag="ACHX5T_004592"
                     /pseudo
     CDS             complement(1..248)
                     /locus_tag="ACHX5T_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           87 a          121 c           86 g           86 t
ORIGIN      
        1 ctgtctccgt tatggcgtac accacatctt ccagtaatcg ccgcttcacc ggactcagga
       61 ccccgtatca ccatccggaa ccccttatcc gccacatgcc aggagaacct ttccataaac
      121 tcgtcgccac tcatcacaag gttttcttct cgttttgtcc ggtggctgtt gtaacgcagg
      181 ccaatttcat cctgaccggc ataatgttcc aggcggctca tctgcaccgg ctgcttctcc
      241 agccagttaa gccatcggtt tccctgctga aaaatatcgg caaaacgggg aagcatcaga
      301 aaggcggaat aacgccatcc ggccagtgaa ccgtaccgca ttcagggcaa tacatgccgt
      361 cggcgttgat accggcagga
//
LOCUS       DAZSIO010000551          377 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZSIO010000551 DAZSIO010000000
VERSION     DAZSIO010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.558"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..>377)
                     /gene="mxiC"
                     /locus_tag="ACHX5T_004593"
     CDS             complement(<1..>377)
                     /gene="mxiC"
                     /locus_tag="ACHX5T_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFR8469432.1"
                     /translation="VNAGINSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYI
                     EWLGNFGFNHRHTIVNFVEQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIF
                     MKKLESSSLLKDGSLSAEQLLLT"
BASE COUNT          125 a           78 c           52 g          122 t
ORIGIN      
        1 aagttagcaa tagctgctct gcacttaaac tgccatcttt tagcaagctt gaggattcca
       61 gtttcttcat aaaaattacg tctgaagttc taatcatctt aattgcaatt aatttagata
      121 acacaaaacc aaactcataa gcattacagc tcggtttctc agaatccatg tctacaatca
      181 gtgactgctc tacaaaattc acaattgtat gtctgtgatt aaaaccaaaa ttaccaagcc
      241 attctatata ctgatctgtt gttgatatgt tccccatgat aaaaccacga taacatgcac
      301 gcaaaagctg tggttcaagt ttcattttgt tgccaaataa tttagcctgt atagctgaat
      361 ttattccagc attaaca
//
LOCUS       DAZSIO010000552          375 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZSIO010000552 DAZSIO010000000
VERSION     DAZSIO010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.559"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>375
                     /locus_tag="ACHX5T_004594"
     CDS             <1..>375
                     /locus_tag="ACHX5T_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR8469433.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRLMRQNALLLERKLAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDRKALHWAVTT"
BASE COUNT           86 a           96 c          110 g           83 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggctca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aactcgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgta aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DAZSIO010000553          374 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZSIO010000553 DAZSIO010000000
VERSION     DAZSIO010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.560"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>374
                     /locus_tag="ACHX5T_004595"
     CDS             <1..>374
                     /locus_tag="ACHX5T_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR8469434.1"
                     /translation="DVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALE
                     MAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRS
                     LKNEWMPVVGYVSFSEAAHAIT"
BASE COUNT           89 a           91 c          121 g           73 t
ORIGIN      
        1 gatgtgacct atatctggac gggtaagcgc tgggcgtacc tcgccgttgt tctcgacctg
       61 ttcgcaagaa aaccagtggg ctgggccatg tcgttctcgc cggacagcag gctcaccatg
      121 aaagcgctgg aaatggcatg ggaaacccgt ggtaagcccg gcggggtgat gttccacagc
      181 gatcagggca gtcattatac gagcaggcag ttccggcagt tattgtggcg ataccagatc
      241 agacagagta tgagccggcg cggaaactgc tgggataaca gcccaatgga acgcttcttc
      301 aggagtctga agaacgaatg gatgccggtg gtgggttacg taagcttcag cgaggcagct
      361 cacgccataa cgga
//
LOCUS       DAZSIO010000554          372 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZSIO010000554 DAZSIO010000000
VERSION     DAZSIO010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.561"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..224)
                     /locus_tag="ACHX5T_004596"
     CDS             complement(<1..224)
                     /locus_tag="ACHX5T_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8469435.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT           96 a           91 c           85 g          100 t
ORIGIN      
        1 gcagcaagtt cagaggcagg aacgacctgt tctcctgcgg ccacagcagt aagacttccc
       61 tcctggtatt gcttgcgcca gagaaataac tggctggctg ccacaccgtg ttgccgggca
      121 acaagggaga ccgtcattcc cggttcaaaa ctctgctgaa caatagcgat cttttcctgt
      181 gtagtacgcc gtctgcgttt ctccggtcct aagacatcaa tcatctgctc tccaatgact
      241 agtctaaaaa ctagtattaa gactatcact taaataagtg atattggttg tctggagatt
      301 cagggggcca gtctacaggt tacaaagatt ctgatcgtct gaactatatt gaaatcggta
      361 cctggtatta ct
//
LOCUS       DAZSIO010000555          372 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZSIO010000555 DAZSIO010000000
VERSION     DAZSIO010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.562"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..311)
                     /locus_tag="ACHX5T_004597"
     CDS             complement(<1..311)
                     /locus_tag="ACHX5T_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8469436.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAZSIO010000556          369 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZSIO010000556 DAZSIO010000000
VERSION     DAZSIO010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.563"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..369
                     /locus_tag="ACHX5T_004598"
                     /pseudo
     CDS             1..369
                     /locus_tag="ACHX5T_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
BASE COUNT           79 a           95 c          115 g           80 t
ORIGIN      
        1 aacccggtac gcaccagaaa atcattgata tggccatgaa tggcgttgga tgccgggcaa
       61 ccgcccgcat tatgggcgtt ggcctcaaca cgattttccg ccatttaaaa aactcaggcc
      121 gcagtcggta acctcgcgca tacagccggg cagtgacgtc atcgtctgcg cggaaatgga
      181 cgaacagtgg ggatacgtcg gggctaaatc gcgccagcgc tggctgtttt acgcgtatga
      241 caggctccgg aagacggttg ttgcgcacgt attcggtgaa cgcactatgg cgacgctggg
      301 gcgtcttatg agcctgctgt caccctttga cgtggtgata tggatgacgg atggctggcc
      361 gctgtatga
//
LOCUS       DAZSIO010000557          365 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZSIO010000557 DAZSIO010000000
VERSION     DAZSIO010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.564"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>365
                     /locus_tag="ACHX5T_004599"
     CDS             <1..>365
                     /locus_tag="ACHX5T_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFR8469437.1"
                     /translation="HQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQ
                     LWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSEN"
BASE COUNT           76 a          124 c          102 g           63 t
ORIGIN      
        1 tcaccagatt gtgaatcgcc tggacatcat gctgccgggc aatcgcccct tcgttgcccc
       61 cagtgccgtt attcaggccc gccagcgcct gggaagtgag gctgtccgcc gcgtgttcac
      121 gaaaacagcg cagctctggc ataacgccac gccgcatccg cactggtgcg gcctgaccct
      181 gctggccatc gatggtgtgt tctggcgcac accggataca ccagagaacg atgcagcctt
      241 cccccgccag acacatgccg ggaacccggc gctctacccg caggtcaaaa tggtctgcca
      301 gatggaactg accagccatc tgctgacggc tgcagccttc ggcacgatga agaacagcga
      361 aaatg
//
LOCUS       DAZSIO010000558          357 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZSIO010000558 DAZSIO010000000
VERSION     DAZSIO010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.565"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
BASE COUNT          102 a           90 c           75 g           90 t
ORIGIN      
        1 cttacaaagt ctattattaa taccgtagat attcatctta ccctgtgcac caaaagcaaa
       61 aacttatcga cgcgggtttg aaactgatgg caaccgcaaa aatgcctgat gcgctacgct
      121 tatcaggcct acgccatctc tgcaatatat tgaatttgcg tgcttttgta ggcaggataa
      181 ggcgttcacg cagcatccgg catgaataaa gcgcactcaa caatctgaaa cccgccggaa
      241 cggtttatta cgtacatcag gtgaaactga ccgataagcc gctttctttt gggtatagtg
      301 tcgtggacag tcattcatct ttctgcccct ccaaaagcaa aaacccgccg aagcggg
//
LOCUS       DAZSIO010000559          345 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZSIO010000559 DAZSIO010000000
VERSION     DAZSIO010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.566"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(13..285)
                     /locus_tag="ACHX5T_004600"
     CDS             complement(13..285)
                     /locus_tag="ACHX5T_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469438.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT           80 a           80 c           90 g           95 t
ORIGIN      
        1 cggggctgaa aatcaccacc acttcgggtc atccgccttc atctccgctt ctgtttcgta
       61 taaatcaaaa cgacggcagg tatggcagaa cgtgacgtat tcgtacggtg aatctgggta
      121 cattttgtgg aaggtttccc agaaacagac cgtgcaggag ggatgaccgg tgataaagtc
      181 tgcaaatacg gacttataag ggtgattatt ggctctggcg acggctttca gaacctcttt
      241 caccattctg gtgtggactt tctggtgctc caggttgtgt gacatgggaa ctcattctgg
      301 atggttactc tgaaagctca tattctgcca caccccgatt tgcag
//
LOCUS       DAZSIO010000560          345 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZSIO010000560 DAZSIO010000000
VERSION     DAZSIO010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.567"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            229..336
                     /gene="ldrD"
                     /locus_tag="ACHX5T_004601"
     CDS             229..336
                     /gene="ldrD"
                     /locus_tag="ACHX5T_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HFR8469439.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           91 a           81 c          100 g           73 t
ORIGIN      
        1 gagacggatt ccggaacaga aaaacagtcg tagaatcagc gtcaagccgg aaggattccg
       61 gtgaggcgca atgttgcggg ggcttaatcc ctggtggcat tggttgctgg agagagaaaa
      121 cccccgcacg ttgcaggtat gcacctgaca acaccacggg ggccattctt gcatccaaac
      181 aatacaagaa tagccgcgaa acgttgtcat tacaatacag gcggctatat gacgttcgca
      241 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg tattcttgcc
      301 agtatgatcg tgaactggct gaacaagcgg aagtaacgtg ccatg
//
LOCUS       DAZSIO010000561          344 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZSIO010000561 DAZSIO010000000
VERSION     DAZSIO010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.568"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>344
                     /locus_tag="ACHX5T_004602"
     CDS             <1..>344
                     /locus_tag="ACHX5T_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR8469440.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAZSIO010000562          343 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZSIO010000562 DAZSIO010000000
VERSION     DAZSIO010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.569"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>343
                     /locus_tag="ACHX5T_004603"
     CDS             <1..>343
                     /locus_tag="ACHX5T_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469441.1"
                     /translation="ATKALQKNVLRVTNSSSSGISEKHLDHCANTVKNFLRKSIAAQS
                     YSKMFSQGTSFKSLNLSLEAPSGARSSFRSLEHLDKVSRHYISEIIQKVHPLSSDERH
                     LLSIIINSNFN"
BASE COUNT          115 a           69 c           51 g          108 t
ORIGIN      
        1 aagcaacaaa agcattacaa aaaaatgtat tacgcgtaac aaactcatct tcttctggca
       61 taagtgaaaa acatttagat cactgtgcta atactgtgaa aaacttttta aggaagtcta
      121 tagcagcgca aagttatagc aaaatgtttt ctcaagggac cagtttcaaa tcactaaatc
      181 tttctcttga agcgccatct ggagcacggt catcatttcg gtcacttgag catctcgata
      241 aggtgtccag gcactatatc tctgagataa tacaaaaagt tcatcctctt tcttcagacg
      301 aacgtcactt actttctatt attattaatt ctaattttaa ctt
//
LOCUS       DAZSIO010000563          341 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZSIO010000563 DAZSIO010000000
VERSION     DAZSIO010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.570"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..171
                     /gene="xisR"
                     /locus_tag="ACHX5T_004604"
     CDS             <1..171
                     /gene="xisR"
                     /locus_tag="ACHX5T_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFR8469442.1"
                     /translation="LMLRTGLGARQIEAYRQNCWVEGFHFKRVSPLGKPDSKRGIIWY
                     NYPKINQFIKDS"
     gene            173..>341
                     /locus_tag="ACHX5T_004605"
     CDS             173..>341
                     /locus_tag="ACHX5T_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFR8469443.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHQISS"
BASE COUNT          108 a           64 c           83 g           86 t
ORIGIN      
        1 ctgatgcttc gcactggtct gggtgccaga caaattgaag cataccgcca gaactgttgg
       61 gtggaaggct tccacttcaa acgagtatct cctttaggga agccagacag taagcgaggg
      121 attatctggt acaactatcc gaagataaat cagtttatca aagactcatg atatgtctaa
      181 attaccaaca ggtgtcgaga ttcgaggtaa atacattcgc atctggttca tgtttcgagg
      241 aaaacgatgt cgggaaacat tgaaaggctg ggagattact aacagtaaca ttaaaaaggc
      301 cgggaattta agagcgttga tagttcatca aatcagttcc g
//
LOCUS       DAZSIO010000564          340 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZSIO010000564 DAZSIO010000000
VERSION     DAZSIO010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.571"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..>340)
                     /locus_tag="ACHX5T_004606"
     CDS             complement(<1..>340)
                     /locus_tag="ACHX5T_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR8469444.1"
                     /translation="IIAIDAIFREIVKNNIVILIISLLSFMLSQTVLNHNPLLDPQWF
                     WNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSITAIMSAYQLLNQKPLLF
                     KIISIFNADII"
BASE COUNT          119 a           53 c           59 g          109 t
ORIGIN      
        1 tattatgtct gcattgaata ttgagataat cttaaaaagt aaaggtttct ggttaagtag
       61 ttgatacgca ctcattatag cggtaattga gaagacaatg aaaccaattt tagattttcc
      121 aaaaaacctg tcacgtgtta attctaaaaa catacatcta ccgagaggca ataaccacca
      181 gtatgccata gcggagtcta tgttccaaaa ccattgagga tctagtaatg ggttatgatt
      241 gagcactgtt tgtgacaaca tgaacgataa cagtgatatg attaaaatca ctatattgtt
      301 cttgactatt tctctaaaga ttgcatcaat tgctattata
//
LOCUS       DAZSIO010000565          339 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZSIO010000565 DAZSIO010000000
VERSION     DAZSIO010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.572"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>339
                     /locus_tag="ACHX5T_004607"
     CDS             <1..>339
                     /locus_tag="ACHX5T_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469445.1"
                     /translation="SLAFDSFKERLESTMAALTFFISTGQCDCDEIAESNFNRMIAYM
                     SNINYDASKLGAPALSFDTYLQDNVKYRVIINNLYGSEFRIRDINENFVGMDFTCEFR
                     PEKMTNLINI"
BASE COUNT          107 a           56 c           71 g          105 t
ORIGIN      
        1 aaagcctggc tttcgactct tttaaagaaa ggcttgagtc taccatggcg gcactcactt
       61 tctttatatc aacgggtcaa tgtgactgcg atgaaatagc agagtcgaac ttcaatcgta
      121 tgattgccta tatgtctaac attaactatg atgcctcaaa gctaggagcg ccagcactta
      181 gttttgatac gtatctgcag gacaatgtta aatatagagt gataattaat aatctttatg
      241 gcagtgagtt tcggataaga gatattaatg agaattttgt tgggatggat tttacctgtg
      301 aattccgacc cgaaaaaatg acgaatttga tcaatataa
//
LOCUS       DAZSIO010000566          333 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZSIO010000566 DAZSIO010000000
VERSION     DAZSIO010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.573"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(43..330)
                     /locus_tag="ACHX5T_004608"
     CDS             complement(43..330)
                     /locus_tag="ACHX5T_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469446.1"
                     /translation="MDEEYRKDLQLWFGLTHASFCVMPRVFMEAMPPEWQEKMAQLLF
                     EYSDTIKTDVCGVHSCFVTAKDGNNRFMRMPEDILNYRHPRREFIESFLKK"
BASE COUNT           91 a           85 c           67 g           90 t
ORIGIN      
        1 taagccatta tcaaacccac ccgtagatag gctttgtaat ggctacttct tcagaaatga
       61 ttcgatgaat tcacgtcgag gatgacgata gttcagaata tcttctggca tcctcataaa
      121 gcggttgttg ccgtctttgg cagtaacaaa acagctgtga actccgcaga catccgtttt
      181 gatcgtgtcg ctatactcaa aaagcaactg agccatcttc tcttgccatt ctggaggcat
      241 agcctccatg aatactcgcg gcatcacgca gaacgacgca tgcgtaagac caaaccacag
      301 ttgcaggtct ttacgatatt cttcatccat cgt
//
LOCUS       DAZSIO010000567          333 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZSIO010000567 DAZSIO010000000
VERSION     DAZSIO010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.574"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..206
                     /locus_tag="ACHX5T_004609"
     CDS             <1..206
                     /locus_tag="ACHX5T_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR8469447.1"
                     /translation="VYIITETVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGL
                     KRGYRLAEQVLMHEPLARMRWCG"
BASE COUNT           83 a           70 c          111 g           69 t
ORIGIN      
        1 tggtgtacat cataacggag acagtgagaa aaacggcgat gcaaatcacc tggagaggga
       61 tgtatcagag gttactgaag gttgacccgc tgaagtgcgt gctgtgcggg agtcagatgc
      121 gttttacggg gctgaagcgg ggctaccgtc tggcagagca ggtcctgatg catgagccgc
      181 tggcccggat gcggtggtgc ggctgagagc cgcagagggg aagttgcgtc cattttgagg
      241 gaaacggagc aaaaaaccgc cattcatacc ctgaatcaat cagagccatc cagtttaatc
      301 gtcggttccg ttcatatgga gcaaaagtgg tgt
//
LOCUS       DAZSIO010000568          332 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZSIO010000568 DAZSIO010000000
VERSION     DAZSIO010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.575"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            51..>332
                     /locus_tag="ACHX5T_004610"
     CDS             51..>332
                     /locus_tag="ACHX5T_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR8469448.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPD"
BASE COUNT           83 a           70 c           98 g           81 t
ORIGIN      
        1 cgtccgaatg ttagccattc gagggttgtt aagtagtttc aggaggattt atggcgaaac
       61 cttttactcc cgaacagcgg gaagaactga agacgcgaat tgtggaactc gtgcatcagg
      121 acggtcgggt cacgattcgg cagttgtccg atgaaacagg tatcagtcgt gcgtctgtcg
      181 gtcgcttatg catagaactg gtcgcaagtg gtgatgtata taattctggc tacggcttat
      241 tcccgtctga acaggctcgc aaggactggc aaagcgcccg caaaaaactc tcgagagtaa
      301 aggtgaggaa accggttgtt gttgatccgg ac
//
LOCUS       DAZSIO010000569          332 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZSIO010000569 DAZSIO010000000
VERSION     DAZSIO010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.576"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>332
                     /locus_tag="ACHX5T_004611"
     CDS             <1..>332
                     /locus_tag="ACHX5T_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8469449.1"
                     /translation="QYLLSLVPYCPWQHIVFTLPCQYWSLVFHNRWLLAEMSRIAADV
                     ILEICHQADVEPGIFTVIHTWGRDQQWHPHIHLSTTAGGVTSGHTWKNLHFYARKVMS
                     MWRYRITR"
BASE COUNT           71 a           88 c           95 g           78 t
ORIGIN      
        1 tacagtatct gctgagcctg gtcccctact gcccgtggca gcatattgtg ttcacacttc
       61 cctgccagta ctggtccctg gtgttccaca accggtggtt actggcagag atgagccgca
      121 ttgcagcgga tgtgatactg gaaatctgcc atcaggcaga tgtggagccg gggatattca
      181 cggtgatcca cacatggggg cgtgaccagc agtggcatcc gcatatccat ttatcgacaa
      241 ctgccggtgg tgtgacgtcg ggccacacct ggaaaaatct tcatttttac gcccgtaagg
      301 tgatgagcat gtggcgctac cggataacac gg
//
LOCUS       DAZSIO010000570          329 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZSIO010000570 DAZSIO010000000
VERSION     DAZSIO010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.577"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
BASE COUNT          100 a           63 c           63 g          103 t
ORIGIN      
        1 ggtatccctt aaggttatta acttgaatta tgacgcactg atattattca tcaaataata
       61 acaaaatagc cattgcatcg ggttggacaa tttacaaaag aaagataacc catattgtat
      121 aatgcgttta aatatgccgt cttatataaa atgatggtca gattaatatg cggattatct
      181 ttctacgcaa ggagtattta tctctactcc cgtcaatgat tgcatctctt ttctctgcta
      241 acggtgtcgc ggcggccatt gatttatgcc agggatatga tatcaaagcg agttgtcacg
      301 ccagcaggca aagcctttca ggcattacg
//
LOCUS       DAZSIO010000571          329 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZSIO010000571 DAZSIO010000000
VERSION     DAZSIO010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.578"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..>329)
                     /locus_tag="ACHX5T_004612"
     CDS             complement(<1..>329)
                     /locus_tag="ACHX5T_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469450.1"
                     /translation="ETILTLLAAVGANNVSGLCMSFMNGHVDTIKAYGEIVFKTPLTS
                     DKRLYLLAAKDSHDLPGLFFALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLF
                     MALQNGHT"
BASE COUNT           98 a           73 c           50 g          108 t
ORIGIN      
        1 gtgtgcccat tttgaagtgc cataaacaac ccatgcttta ctttcaataa ttctttaatt
       61 tgttcactac taagcatttt cttattaagc agacttccga acatacgaat actatctgca
      121 tgtccattct gcaatgcaaa aaataaccca ggtaaatcat ggctgtcttt cgcagctaat
      181 agatataatc tcttatcgga agttaacggg gttttaaaga ctatttcccc ataggctttt
      241 attgtgtcta catgtccgtt cataaaagac atgcataaac ccgacacatt gtttgcccct
      301 accgcagcaa gcaatgtaag tattgtttc
//
LOCUS       DAZSIO010000572          329 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZSIO010000572 DAZSIO010000000
VERSION     DAZSIO010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.579"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..214
                     /gene="mxiH"
                     /locus_tag="ACHX5T_004613"
     CDS             <1..214
                     /gene="mxiH"
                     /locus_tag="ACHX5T_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HFR8469451.1"
                     /translation="SLSETFDDGTQTLQGELTLALDKLAKNPSNPQLLAEYQSKLSEY
                     TLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            227..>329
                     /gene="mxiI"
                     /locus_tag="ACHX5T_004614"
     CDS             227..>329
                     /gene="mxiI"
                     /locus_tag="ACHX5T_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HFR8469452.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKY"
BASE COUNT          120 a           54 c           58 g           97 t
ORIGIN      
        1 ttcattatct gaaacttttg atgatggaac tcaaacatta caaggtgaac taacattggc
       61 actagataaa ttagctaaaa atccttcgaa tccacagttg ctggctgaat accaaagtaa
      121 attatctgaa tatacattat ataggaacgc gcaatccaat acagtgaaag tgattaagga
      181 tgttgatgct gcaattattc aaaacttcag ataataggga gcattcatga attacattta
      241 tccagtcaat caggttgata ttatcaaagc cagtgatttt caatctcaag agatatcaag
      301 tctggaagac gtcgtgtcgg ctaaatata
//
LOCUS       DAZSIO010000573          326 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZSIO010000573 DAZSIO010000000
VERSION     DAZSIO010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.580"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(205..279)
                     /gene="tnaC"
                     /locus_tag="ACHX5T_004615"
     CDS             complement(205..279)
                     /gene="tnaC"
                     /locus_tag="ACHX5T_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFR8469453.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
BASE COUNT          116 a           60 c           54 g           96 t
ORIGIN      
        1 tattttaatt acagtgatcc ctgtgaatat tacatctgca atatccatta attcaccatg
       61 tatatataca agcaccatca tttactaaaa taagtaaata aacgagagat ggcctttgca
      121 aaaggcaaaa ttaagatata gcaaactaat cgatcacatt gaatctaatc ggtttggctc
      181 tggtgatggc tacagaaggg taaattaagg gcggtgatcg acaatcttat tgtcgatatt
      241 gaaccatttt gaggtcacac atacatgtaa gatattcata atgcacttat cctcgaaaga
      301 cacgccgcta cattattaca ctcaga
//
LOCUS       DAZSIO010000574          325 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZSIO010000574 DAZSIO010000000
VERSION     DAZSIO010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.581"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            103..>325
                     /locus_tag="ACHX5T_004616"
     CDS             103..>325
                     /locus_tag="ACHX5T_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR8469454.1"
                     /translation="MIYSNQLRYTGKDGVYFHLQGQPDNFLSLSATENMEFDSPMMVT
                     TQNMQSGQTVTDNVMRSPRTISISGVVVVG"
BASE COUNT          110 a           56 c           61 g           98 t
ORIGIN      
        1 aatttacgta gctcacgctt aacagagaaa atacttatat atcaaaggct tggttaaaaa
       61 tgattaaaat gcgctaagtc tttgaaatca aaagagaatt taatgattta ctctaatcag
      121 cttcgttaca ctggcaaaga tggtgtttac ttccatctac aaggccaacc agataatttc
      181 ttgtcactat cagcaactga aaacatggaa tttgatagcc ctatgatggt taccactcaa
      241 aatatgcaat cagggcagac tgtaacagat aatgtaatgc gttcacctag aactatttca
      301 atcagtggtg tggttgtggt tggtg
//
LOCUS       DAZSIO010000575          323 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZSIO010000575 DAZSIO010000000
VERSION     DAZSIO010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.582"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..>323)
                     /locus_tag="ACHX5T_004617"
     rRNA            complement(<1..>323)
                     /locus_tag="ACHX5T_004617"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           61 a          103 c           74 g           85 t
ORIGIN      
        1 cccggggatt tcacatctga cttaacaaac cgcctgcgtg cgctttacgc ccagtaattc
       61 cgattaacgc ttgcaccctc cgtattaccg cggctgctgg cacggagtta gccggtgctt
      121 cttctgcggg taacgtcaat gagcaaaggt attaacttta ctcccttcct ccccgctgaa
      181 agtactttac aacccgaagg ccttcttcat acacgcggca tggctgcatc aggcttgcgc
      241 ccattgtgca atattcccca ctgctgcctc ccgtaggagt ctggaccgtg tctcagttcc
      301 agtgtggctg gtcatcctct cag
//
LOCUS       DAZSIO010000576          321 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZSIO010000576 DAZSIO010000000
VERSION     DAZSIO010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.583"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
BASE COUNT           89 a           57 c           55 g          120 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt a
//
LOCUS       DAZSIO010000577          305 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZSIO010000577 DAZSIO010000000
VERSION     DAZSIO010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.585"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
BASE COUNT           77 a           70 c           74 g           84 t
ORIGIN      
        1 ggagcaatat taggcaaacg atttgagata tgacctaaaa gctcggccaa ataataatgt
       61 cgtcgattat cgaagtcatc atgagtgatt ctgtccccac agtgcagcat cagcatgaca
      121 gtgaattgtc tggcgtggtc catttaatga gatggagatg gtccctcttt cctaaatagc
      181 tgtgcctaat accgctacac ttttgccagc ccatgtttgc ctccggggaa tgggctgacg
      241 gttccttcaa aatccattgc acgcagttat gtcggagcgg gaacttgact cctgtcttta
      301 cggga
//
LOCUS       DAZSIO010000578          299 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZSIO010000578 DAZSIO010000000
VERSION     DAZSIO010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.586"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>299
                     /locus_tag="ACHX5T_004618"
     CDS             <1..>299
                     /locus_tag="ACHX5T_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR8469455.1"
                     /translation="YREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKM
                     AEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTR"
BASE COUNT           90 a           52 c           91 g           66 t
ORIGIN      
        1 gatacaggga gataaaacag acgatgcaac tgagcaggct gacgctgaga agtaaaaagc
       61 cggagcttgt ggagcaagag ctgtggggtg tcttactggc ttataatctg gtgagatatc
      121 agatgattaa aatggcggaa catctgaaag gttactggcc gaatcaactg agtttctcag
      181 aatcatgcgg aatggtgatg agaatgctga tgacattgca gggcgcttca ccgggacgta
      241 taccggagct gatgcgcgat cttgcaagta tgggacaact tgtgaaatta ccgacgaga
//
LOCUS       DAZSIO010000579          289 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZSIO010000579 DAZSIO010000000
VERSION     DAZSIO010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.587"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>289
                     /locus_tag="ACHX5T_004619"
     CDS             <1..>289
                     /locus_tag="ACHX5T_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR8469456.1"
                     /translation="TLMDKGYYSLGLLNGWSLAGEHRHWMIPLRKGAQYEELRKLGKG
                     DHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGG"
BASE COUNT           84 a           68 c           89 g           48 t
ORIGIN      
        1 gacgttaatg gataaaggtt attactcact gggactgtta aatggctgga gcctggcggg
       61 agaacaccgc cactggatga tacctctcag aaagggagcg caatatgaag agctcagaaa
      121 actgggtaaa ggcgatcatc tggtgaagct gaaaaccagc ccgcaggcac gaaaaaagtg
      181 gccgggactg ggaaatgaag tgacagcccg cctgctgacc gtgacgcgca aaggaaaagt
      241 ctgccatctg ctgacgtcga tgacggacgc catgcgcttc cccggagga
//
LOCUS       DAZSIO010000580          288 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZSIO010000580 DAZSIO010000000
VERSION     DAZSIO010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.588"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(31..>288)
                     /locus_tag="ACHX5T_004620"
     CDS             complement(31..>288)
                     /locus_tag="ACHX5T_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HFR8469457.1"
                     /translation="MLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQI
                     EKIYPIVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
BASE COUNT           95 a           59 c           38 g           96 t
ORIGIN      
        1 gctctttaat aataaataaa tctatcaaag ctaaatatta aatggaagcc accctttttt
       61 ctctgatagt tttattatca ccaaaccatt tatttcagct atcgaatctg gattcggcca
      121 ggaaggtaaa ctcttcacaa ttggataaat cttctctatt tgttctttat ttgctggttt
      181 gcaattacag attccatagt aattcataaa tgcaaccatc ctaaaatagt tgccaccatc
      241 ccaccaaaaa aaagattttc caaaagtgtc tgcgctcatg gagagcat
//
LOCUS       DAZSIO010000581          284 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZSIO010000581 DAZSIO010000000
VERSION     DAZSIO010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.589"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            1..284
                     /gene="ydjO"
                     /locus_tag="ACHX5T_004621"
                     /pseudo
     CDS             1..284
                     /gene="ydjO"
                     /locus_tag="ACHX5T_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="protein YdjO"
BASE COUNT           82 a           49 c           55 g           98 t
ORIGIN      
        1 tatatacatc ctgccgggag catttttgct gcgattcaaa aaaaatgaga gtctcctaaa
       61 gtttccttcg ataatatcaa tgcttaatat caatcttctt tttaatccac taaatcttcc
      121 gggaatggga agtggtgtgc ttgaagatat tatgagtatt ccagatagtt cacttagaaa
      181 acgtctcggg tatgaggttt taagcttctc actacaggca cattcattga gccaggaatg
      241 tattgataag ttggacattt ttttcgctga cgatctgttt aaat
//
LOCUS       DAZSIO010000582          283 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZSIO010000582 DAZSIO010000000
VERSION     DAZSIO010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.590"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..>283)
                     /locus_tag="ACHX5T_004622"
     CDS             complement(<1..>283)
                     /locus_tag="ACHX5T_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR8469458.1"
                     /translation="GSIKELLDYQFSTNEIAAVVAERDIEWWQNRASVLTTPQLASGY
                     FNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNG"
BASE COUNT           91 a           57 c           41 g           94 t
ORIGIN      
        1 ttaccattca ataatacatt cagtacatct tgatcaaggt gggtgatttt acttacccaa
       61 tctgggtcac gcaacatttc aatagctttt gacgaaatgt tatttagatt ccactcatca
      121 atatttatca gtaaaaaacc agcattgaaa tatccagaag ctaactgtgg tgtagttaat
      181 accgaggctc gattttgcca ccattcaata tctctttcag ctacaactgc ggcaatttca
      241 ttagtagaaa attgataatc taagagttct ttaatactac cct
//
LOCUS       DAZSIO010000583          278 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZSIO010000583 DAZSIO010000000
VERSION     DAZSIO010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.591"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            24..100
                     /locus_tag="ACHX5T_004623"
     tRNA            24..100
                     /locus_tag="ACHX5T_004623"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="ACHX5T_004624"
     tRNA            143..218
                     /locus_tag="ACHX5T_004624"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           75 a           62 c           73 g           68 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaag
//
LOCUS       DAZSIO010000584          274 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZSIO010000584 DAZSIO010000000
VERSION     DAZSIO010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.592"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..>274)
                     /locus_tag="ACHX5T_004625"
     CDS             complement(<1..>274)
                     /locus_tag="ACHX5T_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8469459.1"
                     /translation="FSQRNPDNSIVFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGH
                     EMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPL"
BASE COUNT           62 a           75 c           63 g           74 t
ORIGIN      
        1 gcagtggtcg gtaatacaca ccgtcaacgt tatccgctga gttacggccg gtatccgata
       61 tcgccatccc tgacagcccg tgtcccgaag gtgcgcaaaa tctccacgaa acggtgttac
      121 tgccggggct gtaatacatt tcatggccat accgtacatc ctgtaccccc tctgttcgat
      181 atttatagcg ggcatcgaag tttccgtaat ttaacggaat tacctgtccg ttaacagtga
      241 acactatgct gttatccggg tttctctggc tgaa
//
LOCUS       DAZSIO010000585          270 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZSIO010000585 DAZSIO010000000
VERSION     DAZSIO010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.593"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            <1..>270
                     /locus_tag="ACHX5T_004626"
     CDS             <1..>270
                     /locus_tag="ACHX5T_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR8469460.1"
                     /translation="VWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPD
                     ERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLN"
BASE COUNT           68 a           69 c           75 g           58 t
ORIGIN      
        1 gtctggagac tgatgaaacg tgtcggaata aaggctcagg tcggataccg aagcccgcgg
       61 gcacgtaaag gcgaggccag tatcgtgtca cccaacaggc tccagcgaca gttcaatccg
      121 gatgctcctg atgagcgttg ggtaacggac ataacctaca tcaggaccca cgaaggctgg
      181 ctgtatcttg ccgttgttgt tgatctgttc tcacgcaaaa ttatcggctg gtccatgcaa
      241 tcccggatga caaaggacat tgtcctgaac
//
LOCUS       DAZSIO010000586          269 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZSIO010000586 DAZSIO010000000
VERSION     DAZSIO010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.denovo.594"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            complement(<1..>269)
                     /locus_tag="ACHX5T_004627"
     CDS             complement(<1..>269)
                     /locus_tag="ACHX5T_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFR8469461.1"
                     /translation="LKIWFGENYQINSFNILNVLLIGFFFNALAMIPFSAIQALGKSK
                     ITALIHCAELVPYLALLYFMVEKYGLLGAAISWSIRVILDALLLQ"
BASE COUNT          106 a           51 c           39 g           73 t
ORIGIN      
        1 cattgtaata acagagcatc taaaattaca cgtatgctcc aggatattgc ggcacccagt
       61 aacccatatt tttcgaccat aaaatacaaa agggctaaat aaggaaccaa ttcagcacaa
      121 tgaatcaaag cagtaatttt agattttcct aatgcctgga ttgcagagaa tggtatcatt
      181 gccagcgcat tgaagaaaaa accgatcaat aacacattta atatattaaa ggaattaatt
      241 tgataattct caccaaacca tattttcaa
//
LOCUS       DAZSIO010000587         8792 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.guided.155, whole genome shotgun sequence.
ACCESSION   DAZSIO010000587 DAZSIO010000000
VERSION     DAZSIO010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8792
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.guided.155"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            263..922
                     /locus_tag="ACHX5T_004628"
     CDS             263..922
                     /locus_tag="ACHX5T_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFR8469462.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1123..1500)
                     /locus_tag="ACHX5T_004629"
     CDS             complement(1123..1500)
                     /locus_tag="ACHX5T_004629"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8469463.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1567..4533)
                     /locus_tag="ACHX5T_004630"
     CDS             complement(1567..4533)
                     /locus_tag="ACHX5T_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFR8469464.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4536..5096)
                     /locus_tag="ACHX5T_004631"
     CDS             complement(4536..5096)
                     /locus_tag="ACHX5T_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR8469465.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5222..5572)
                     /locus_tag="ACHX5T_004632"
                     /pseudo
     CDS             complement(5222..5572)
                     /locus_tag="ACHX5T_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(5775..6788)
                     /gene="intI1"
                     /locus_tag="ACHX5T_004633"
     CDS             complement(5775..6788)
                     /gene="intI1"
                     /locus_tag="ACHX5T_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR8469466.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            6998..7828
                     /locus_tag="ACHX5T_004634"
     CDS             6998..7828
                     /locus_tag="ACHX5T_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HFR8469467.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            7941..8779
                     /gene="aadA1"
                     /locus_tag="ACHX5T_004635"
                     /pseudo
     CDS             7941..8779
                     /gene="aadA1"
                     /locus_tag="ACHX5T_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT         2000 a         2357 c         2482 g         1953 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata
       61 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      121 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
      181 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      241 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      301 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      361 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      421 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      481 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      541 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      601 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      661 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      721 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      781 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      841 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      901 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
      961 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc
     1021 gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt
     1081 accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag
     1141 tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc
     1201 cagagcctga taaaaacggt tagcgccggg gttggatttt tcagcgttcc agctaaggct
     1261 aaggcattcc tgttcaagcg caagccgggc tataaagcgc attatcgctt tacccgtgcc
     1321 cttatttcga tcgcactgag aaacatacag ctctttaata tgcagctgac cgctgtatcg
     1381 gggcgaggga taaagaatat tgcaacatgc caggccagta atgttattgc cgcagcgcgc
     1441 tctgatcacc agggtaccgg aaagccggtt gaataacttt ttacagagat aatccttcat
     1501 caacgcttcc tgaatgatac cttcgccata ataggggtcg tctcagaaaa cggaaaataa
     1561 agcacgctaa gccggttgca gaggccgtag cggcctgaac ttccccgcgc cgatcttggc
     1621 gctgctgcgc cataggtaat caccggtcag gttgatgtgc tcccagccga gtggcgacag
     1681 gtactgcaat agcgagtcat cgacggcatg accattgccg cgcaacgcat gcgccgcacg
     1741 ctccaggtag accgtgttcc acagcacgat ggccgccgtc accaggttga ggccgctggc
     1801 ccggtagcgc tgctgctcga aactgcggtc acggatttca ccaaggcggt tgaagaacac
     1861 ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc ccggcatgca cgcggcggcg
     1921 tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg cgctcgatgc ggcccaactc
     1981 gcgcagcgcg acggccaagc cgttctggcg cgggtagctg ccgagtttcc tgagcatcag
     2041 cgaggccgtc accgtgccct gcttgatcga ggtggccagc cgcaggattt cgtcccaatg
     2101 ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatc ggcttgagcg cgtcataggc
     2161 ggcatcgccc ttcgggatgt agagcttggt gtcgcccagg tcgcggatgc gcggcgcgaa
     2221 gcggaagccc aagaggtgca tcagggcgaa gacgtgatcg gtgaagcccg ccgtgtcggt
     2281 gtagtgctcc tcgatccgca ggtcggattc gtggtacagc aggccgtcga gcacgtaggt
     2341 tgagtcgcgc aggccgacat tgaccacctt ggtgtggaat ggcgcgtatt ggtcggagat
     2401 gtgggtgtag aaagtccgtc ctgggctgct gccatatttt gggttgatgt gccccgtgct
     2461 ctttgccttg ctagcggttc ggaaattctg tccgtccgat gatgatgtgg tgccatcgcc
     2521 ccagtgcccg gcaaagggat gccgaaactg agcgttgacc agttcagcca acgctgtcga
     2581 gtacgtttcg tcgcgggtat gccaggcttg cagccaagcg agcttcgcgt aggtcgtgcc
     2641 ggggcaggac tcggccatct tggtcaggcc caggttgatc gcgtcggcca ggatcgtggt
     2701 caacaacagg ttcttgtcct tggccagatc gcccgatttc aagtgcgtga agtgccgggt
     2761 gaagcccgtc cactcatcga cttcgagcag cagttcggtg atcttgacgt gcggcaggac
     2821 catggctgtc tggtctatca gcgcctgcgc ggtgtcgggc accgccgcat ccagcggcgt
     2881 gatcttcaag cccgactcgg tgatgatggc atccggcagg tcgttggctg ccgccatgcg
     2941 gttgacggtg gcaagttgtg cttccagcag cgtcagccgc tcatgcagat attgttcgca
     3001 gtcggtggcc acggccagcg gcaattcgct ggactgcttg aggctggtga acttctcggg
     3061 cggtaccagg tagtcctcga agtccttgaa ctggcgtgaa ccctgcaccc agatgtcgcc
     3121 cgagcgcagg gagttcttca actcggacag cgcgcacagt tcgtagtagc gccggtcgat
     3181 gccggcgtcg gtcatcacca gtttctgcca gcgcggcttg atgaagccgg tcggtgcatc
     3241 ggctggcagc ttgcgggcgt tgtcggtgtt catgccgcgc agcacctcaa tggcatcaag
     3301 cacgtttttg gcggcgggcg cggcccgcag cttgagcacg gcaaggaatt ccggtgcata
     3361 gcggcgcagg gtggcgtagc tctcgccgat gcgatgcagg aaatcgaagt catcgggttg
     3421 cgcgagcttc tgcgcctcgg tgacgctctc ggcaaaggaa tcccaggaca tgacggcctc
     3481 gatggcggca aacgcatcgc ggcctgattg cttggcgtcg atcagcgcct gaccgatgcg
     3541 cccgtacaga cgtaccttgg cgttgatggc cttgcctgac gcctggaact gctgctgatg
     3601 cttattcttg gcagcgttaa acagcttacc caggatgcgg tcgtgcaggt cgatgatttc
     3661 gtcggtgacg gtggccatgc cctcggtggc cagcgccacg agagtggcgt agcgccgttg
     3721 cggctcgaat ttggccaggt cggcgggtgt catctggccg ccctcgcggg caatcttgag
     3781 caggcggttc tggtgaacca gccgctcgat gccggtaggc agatcgagtg cctgccatgc
     3841 cttgaggcgt tcgatgtgtt ccagcatatg ccgcgaattt ggcttggccg gagactggcg
     3901 caaccaagcc aaccaggtcg tcttgccgtt gtcccggcgc ttgagcagat cgtcgaggcg
     3961 gcggcgatgc gcgtccgcca gtggttcggc caaggcgtcg tagatgcgcc ggttagcacg
     4021 ggtgatcgcc tcggcactcg cccgctcgac ggcgttgagg gcgggcagaa tgaccgactg
     4081 ccgccgcagg tgcccgatca aggcgctggc cagcacgatg cctttgtcgg tttgcatcgc
     4141 cagctcggtc agcatctgga cggcctgccg gtaatggctc atggtgaagg gccggaaacc
     4201 gaacacggtt tgcagctcgc tcaggtgctc gcgccgggtc tgctcccgct ggccgtactc
     4261 gttccagctt tcgacgccga ccttgagctg gtcggcgacc agcttcaaca agggcgggaa
     4321 cggtagttca tcgacgccca ggatgacgcc gggaaagcgc aggtaacaga gctgcaccgc
     4381 gaagcccagc cgattggctg gcccgcgccg ctgtcggatg atcgagaggt cggtatcgtt
     4441 gaatgtgtaa tgtcggatca ggtcgtcctt ggagtccggc aacgccagca ggctttcccg
     4501 ctcggcggcg gacaggatgg aacgacgtgg catatttact gatccgttct caagtattga
     4561 tacagggttt cgcgactgat tccgaattca cgagcaagct tggtcttttg ctcgccagcc
     4621 tcgacacgtt ggcgcagttc ggcaatacgc tcagacgaca gggatttctt cctgccacgg
     4681 taagccccgc gttgcttggc gagcgcaata ccctcgcgct gacgctcgcg gatcagggcg
     4741 cgctcgaact cggcgaacgc gcccatcacc gagagcatca ggttcgccat cggagagtct
     4801 tcgccagtaa aactgaggtg ttccttgacg aattcgatat gcacgccgcg ttgtgtcagc
     4861 gtttgcacga tccggcgcaa atcatcgaga ttgcgcgcca ggcgatccat gctatgcacc
     4921 accacggtgt cgccggtgcg ggcgaagctt atcagcgctt ccagttgcgg acgcttgaca
     4981 tccttgccgg atgccttgtc gctaaaagcg cgatcaacct tgacgccttc cagttgccgt
     5041 tccgggttct ggtcgaaggt gctgaccctg atatacccaa tgcgctgtcc agtcatggaa
     5101 ttccctgcaa aatgtcaggg aagactctat gaccttcaac gagatatgtc aataaattca
     5161 aaattcaatc ctatcctgac gcaatttaca catggcatct gacatcaggt tagggtatgc
     5221 ctcaacctga cggcggcgaa tcacaagcgt ccggtttgac gctggtttcg gtcgcagtgc
     5281 tggcccgcgc ctggaagcgc tgatagcact ccagcccgca gaaatgctcc acgtactcgg
     5341 ccccttccgg cgtgaaggcg gcatcgagcg ggatttcctt gcagcacacg cagcaactgg
     5401 tgctcggttc attggcgttc atggtggtgt tcctccattg gttgacgaag ccgacgaagg
     5461 ccgccgccgg catcggcctg gcgaacagga aaccctgcac cgtgtcgcaa cccgcctgcc
     5521 gcaaccacgc aaggcagtcg ggtgtttcca caccctcggc taccacctcc attgtcgttt
     5581 tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg
     5641 atccatcagg caacgacggg ctgctgccgg ccatcagcgg acgcagggag gactttccgc
     5701 aaccggccgt tcgatgcggc accgatggcc ttcgcgcagg ggtagtgaat ccgccaggat
     5761 tgacttgcgc tgccctacct ctcactagtg aggggcggca gcgcatcaag cggtgagcgc
     5821 actccggcac cgccaacttt cagcacatgc gtgtaaatca tcgtcgtaga gacgtcggaa
     5881 tggccgagca gatcctgcac ggttcgaatg tcgtaaccgc tgcggagcaa ggccgtcgcg
     5941 aacgagtggc ggagggtgtg cggtgtggcg ggcttcgtga tgcctgcttg ttctacggca
     6001 cgtttgaagg cgcgctgaaa ggtctggtca tacatgtgat ggcgacgcac gacaccgctc
     6061 cgtggatcgg tcgaatgcgt gtgctgcgca aaaacccaga accacggcca ggaatgcccg
     6121 gcgcgcggat acttccgctc aagggcgtcg ggaagcgcaa cgccgctgcg gccctcggcc
     6181 tggtccttca gccaccatgc ccgtgcacgc gacagctgct cgcgcaggct gggtgccaag
     6241 ctctcgggta acatcaaggc ccgatccttg gagcccttgc cctcccgcac gatgatcgtg
     6301 ccgtgatcga aatccagatc cttgacccgc agttgcaaac cctcactgat ccgcatgccc
     6361 gttccataca gaagctgggc gaacaaacga tgctcgcctt ccagaaaacc gaggatgcga
     6421 accacttcat ccggggtcag caccaccggc aagcgccgcg acggccgagg tcttccgatc
     6481 tcctgaagcc agggcagatc cgtgcacagc accttgccgt agaagaacag caaggccgcc
     6541 aatgcctgac gatgcgtgga gaccgaaacc ttgcgctcgt tcgccagcca ggacagaaat
     6601 gcctcgactt cgctgctgcc caaggttgcc gggtgacgca caccgtggaa acggatgaag
     6661 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     6721 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     6781 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     6841 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     6901 cagcagggca gtcgccctaa aacaaagttg ggcgaacccg gagcctcatt aattgttagc
     6961 cgttaaaatt aagcccttta ccaaaccaat acttattatg aaaaacacaa tacatatcaa
     7021 cttcgctatt tttttaataa ttgcaaatat tatctacagc agcgccagtg catcaacaga
     7081 tatctctact gttgcatctc cattatttga aggaactgaa ggttgttttt tactttacga
     7141 tgcatccaca aacgctgaaa ttgctcaatt caataaagca aagtgtgcaa cgcaaatggc
     7201 accagattca actttcaaga tcgcattatc acttatggca tttgatgcgg aaataataga
     7261 tcagaaaacc atattcaaat gggataaaac ccccaaagga atggagatct ggaacagcaa
     7321 tcatacacca aagacgtgga tgcaattttc tgttgtttgg gtttcgcaag aaataaccca
     7381 aaaaattgga ttaaataaaa tcaagaatta tctcaaagat tttgattatg gaaatcaaga
     7441 cttctctgga gataaagaaa gaaacaacgg attaacagaa gcatggctcg aaagtagctt
     7501 aaaaatttca ccagaagaac aaattcaatt cctgcgtaaa attattaatc acaatctccc
     7561 agttaaaaac tcagccatag aaaacaccat agagaacatg tatctacaag atctggataa
     7621 tagtacaaaa ctgtatggga aaactggtgc aggattcaca gcaaatagaa ccttacaaaa
     7681 cggatggttt gaagggttta ttataagcaa atcaggacat aaatatgttt ttgtgtccgc
     7741 acttacagga aacttggggt cgaatttaac atcaagcata aaagccaaga aaaatgcgat
     7801 caccattcta aacacactaa atttataaaa aatctaatgg caaaatcgcc caacccttca
     7861 atcaagtcgg gacggccaaa agcaagcttt tggctcccct cgctggcgct cggcgcccct
     7921 tatttcaaac gttaaacatc atgagggaag tggtgatcgc cgaagtatcg actcaactat
     7981 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     8041 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     8101 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     8161 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     8221 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     8281 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     8341 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     8401 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     8461 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     8521 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     8581 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     8641 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtt cactacgtga
     8701 aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgacgcc
     8761 gcttcgcggc gcggcttaac tcaagcgtta ga
//
LOCUS       DAZSIO010000588         8789 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.guided.156, whole genome shotgun sequence.
ACCESSION   DAZSIO010000588 DAZSIO010000000
VERSION     DAZSIO010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.guided.156"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            263..922
                     /locus_tag="ACHX5T_004636"
     CDS             263..922
                     /locus_tag="ACHX5T_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFR8469468.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1123..1500)
                     /locus_tag="ACHX5T_004637"
     CDS             complement(1123..1500)
                     /locus_tag="ACHX5T_004637"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8469469.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1567..4533)
                     /locus_tag="ACHX5T_004638"
     CDS             complement(1567..4533)
                     /locus_tag="ACHX5T_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFR8469470.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4536..5096)
                     /locus_tag="ACHX5T_004639"
     CDS             complement(4536..5096)
                     /locus_tag="ACHX5T_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR8469471.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5222..5572)
                     /locus_tag="ACHX5T_004640"
                     /pseudo
     CDS             complement(5222..5572)
                     /locus_tag="ACHX5T_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(5775..6788)
                     /gene="intI1"
                     /locus_tag="ACHX5T_004641"
     CDS             complement(5775..6788)
                     /gene="intI1"
                     /locus_tag="ACHX5T_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR8469472.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            6998..7828
                     /locus_tag="ACHX5T_004642"
     CDS             6998..7828
                     /locus_tag="ACHX5T_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HFR8469473.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            7941..8776
                     /gene="aadA1"
                     /locus_tag="ACHX5T_004643"
                     /pseudo
     CDS             7941..8776
                     /gene="aadA1"
                     /locus_tag="ACHX5T_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT         1997 a         2357 c         2482 g         1953 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata
       61 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      121 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
      181 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      241 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      301 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      361 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      421 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      481 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      541 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      601 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      661 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      721 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      781 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      841 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      901 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
      961 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc
     1021 gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt
     1081 accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag
     1141 tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc
     1201 cagagcctga taaaaacggt tagcgccggg gttggatttt tcagcgttcc agctaaggct
     1261 aaggcattcc tgttcaagcg caagccgggc tataaagcgc attatcgctt tacccgtgcc
     1321 cttatttcga tcgcactgag aaacatacag ctctttaata tgcagctgac cgctgtatcg
     1381 gggcgaggga taaagaatat tgcaacatgc caggccagta atgttattgc cgcagcgcgc
     1441 tctgatcacc agggtaccgg aaagccggtt gaataacttt ttacagagat aatccttcat
     1501 caacgcttcc tgaatgatac cttcgccata ataggggtcg tctcagaaaa cggaaaataa
     1561 agcacgctaa gccggttgca gaggccgtag cggcctgaac ttccccgcgc cgatcttggc
     1621 gctgctgcgc cataggtaat caccggtcag gttgatgtgc tcccagccga gtggcgacag
     1681 gtactgcaat agcgagtcat cgacggcatg accattgccg cgcaacgcat gcgccgcacg
     1741 ctccaggtag accgtgttcc acagcacgat ggccgccgtc accaggttga ggccgctggc
     1801 ccggtagcgc tgctgctcga aactgcggtc acggatttca ccaaggcggt tgaagaacac
     1861 ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc ccggcatgca cgcggcggcg
     1921 tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg cgctcgatgc ggcccaactc
     1981 gcgcagcgcg acggccaagc cgttctggcg cgggtagctg ccgagtttcc tgagcatcag
     2041 cgaggccgtc accgtgccct gcttgatcga ggtggccagc cgcaggattt cgtcccaatg
     2101 ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatc ggcttgagcg cgtcataggc
     2161 ggcatcgccc ttcgggatgt agagcttggt gtcgcccagg tcgcggatgc gcggcgcgaa
     2221 gcggaagccc aagaggtgca tcagggcgaa gacgtgatcg gtgaagcccg ccgtgtcggt
     2281 gtagtgctcc tcgatccgca ggtcggattc gtggtacagc aggccgtcga gcacgtaggt
     2341 tgagtcgcgc aggccgacat tgaccacctt ggtgtggaat ggcgcgtatt ggtcggagat
     2401 gtgggtgtag aaagtccgtc ctgggctgct gccatatttt gggttgatgt gccccgtgct
     2461 ctttgccttg ctagcggttc ggaaattctg tccgtccgat gatgatgtgg tgccatcgcc
     2521 ccagtgcccg gcaaagggat gccgaaactg agcgttgacc agttcagcca acgctgtcga
     2581 gtacgtttcg tcgcgggtat gccaggcttg cagccaagcg agcttcgcgt aggtcgtgcc
     2641 ggggcaggac tcggccatct tggtcaggcc caggttgatc gcgtcggcca ggatcgtggt
     2701 caacaacagg ttcttgtcct tggccagatc gcccgatttc aagtgcgtga agtgccgggt
     2761 gaagcccgtc cactcatcga cttcgagcag cagttcggtg atcttgacgt gcggcaggac
     2821 catggctgtc tggtctatca gcgcctgcgc ggtgtcgggc accgccgcat ccagcggcgt
     2881 gatcttcaag cccgactcgg tgatgatggc atccggcagg tcgttggctg ccgccatgcg
     2941 gttgacggtg gcaagttgtg cttccagcag cgtcagccgc tcatgcagat attgttcgca
     3001 gtcggtggcc acggccagcg gcaattcgct ggactgcttg aggctggtga acttctcggg
     3061 cggtaccagg tagtcctcga agtccttgaa ctggcgtgaa ccctgcaccc agatgtcgcc
     3121 cgagcgcagg gagttcttca actcggacag cgcgcacagt tcgtagtagc gccggtcgat
     3181 gccggcgtcg gtcatcacca gtttctgcca gcgcggcttg atgaagccgg tcggtgcatc
     3241 ggctggcagc ttgcgggcgt tgtcggtgtt catgccgcgc agcacctcaa tggcatcaag
     3301 cacgtttttg gcggcgggcg cggcccgcag cttgagcacg gcaaggaatt ccggtgcata
     3361 gcggcgcagg gtggcgtagc tctcgccgat gcgatgcagg aaatcgaagt catcgggttg
     3421 cgcgagcttc tgcgcctcgg tgacgctctc ggcaaaggaa tcccaggaca tgacggcctc
     3481 gatggcggca aacgcatcgc ggcctgattg cttggcgtcg atcagcgcct gaccgatgcg
     3541 cccgtacaga cgtaccttgg cgttgatggc cttgcctgac gcctggaact gctgctgatg
     3601 cttattcttg gcagcgttaa acagcttacc caggatgcgg tcgtgcaggt cgatgatttc
     3661 gtcggtgacg gtggccatgc cctcggtggc cagcgccacg agagtggcgt agcgccgttg
     3721 cggctcgaat ttggccaggt cggcgggtgt catctggccg ccctcgcggg caatcttgag
     3781 caggcggttc tggtgaacca gccgctcgat gccggtaggc agatcgagtg cctgccatgc
     3841 cttgaggcgt tcgatgtgtt ccagcatatg ccgcgaattt ggcttggccg gagactggcg
     3901 caaccaagcc aaccaggtcg tcttgccgtt gtcccggcgc ttgagcagat cgtcgaggcg
     3961 gcggcgatgc gcgtccgcca gtggttcggc caaggcgtcg tagatgcgcc ggttagcacg
     4021 ggtgatcgcc tcggcactcg cccgctcgac ggcgttgagg gcgggcagaa tgaccgactg
     4081 ccgccgcagg tgcccgatca aggcgctggc cagcacgatg cctttgtcgg tttgcatcgc
     4141 cagctcggtc agcatctgga cggcctgccg gtaatggctc atggtgaagg gccggaaacc
     4201 gaacacggtt tgcagctcgc tcaggtgctc gcgccgggtc tgctcccgct ggccgtactc
     4261 gttccagctt tcgacgccga ccttgagctg gtcggcgacc agcttcaaca agggcgggaa
     4321 cggtagttca tcgacgccca ggatgacgcc gggaaagcgc aggtaacaga gctgcaccgc
     4381 gaagcccagc cgattggctg gcccgcgccg ctgtcggatg atcgagaggt cggtatcgtt
     4441 gaatgtgtaa tgtcggatca ggtcgtcctt ggagtccggc aacgccagca ggctttcccg
     4501 ctcggcggcg gacaggatgg aacgacgtgg catatttact gatccgttct caagtattga
     4561 tacagggttt cgcgactgat tccgaattca cgagcaagct tggtcttttg ctcgccagcc
     4621 tcgacacgtt ggcgcagttc ggcaatacgc tcagacgaca gggatttctt cctgccacgg
     4681 taagccccgc gttgcttggc gagcgcaata ccctcgcgct gacgctcgcg gatcagggcg
     4741 cgctcgaact cggcgaacgc gcccatcacc gagagcatca ggttcgccat cggagagtct
     4801 tcgccagtaa aactgaggtg ttccttgacg aattcgatat gcacgccgcg ttgtgtcagc
     4861 gtttgcacga tccggcgcaa atcatcgaga ttgcgcgcca ggcgatccat gctatgcacc
     4921 accacggtgt cgccggtgcg ggcgaagctt atcagcgctt ccagttgcgg acgcttgaca
     4981 tccttgccgg atgccttgtc gctaaaagcg cgatcaacct tgacgccttc cagttgccgt
     5041 tccgggttct ggtcgaaggt gctgaccctg atatacccaa tgcgctgtcc agtcatggaa
     5101 ttccctgcaa aatgtcaggg aagactctat gaccttcaac gagatatgtc aataaattca
     5161 aaattcaatc ctatcctgac gcaatttaca catggcatct gacatcaggt tagggtatgc
     5221 ctcaacctga cggcggcgaa tcacaagcgt ccggtttgac gctggtttcg gtcgcagtgc
     5281 tggcccgcgc ctggaagcgc tgatagcact ccagcccgca gaaatgctcc acgtactcgg
     5341 ccccttccgg cgtgaaggcg gcatcgagcg ggatttcctt gcagcacacg cagcaactgg
     5401 tgctcggttc attggcgttc atggtggtgt tcctccattg gttgacgaag ccgacgaagg
     5461 ccgccgccgg catcggcctg gcgaacagga aaccctgcac cgtgtcgcaa cccgcctgcc
     5521 gcaaccacgc aaggcagtcg ggtgtttcca caccctcggc taccacctcc attgtcgttt
     5581 tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg
     5641 atccatcagg caacgacggg ctgctgccgg ccatcagcgg acgcagggag gactttccgc
     5701 aaccggccgt tcgatgcggc accgatggcc ttcgcgcagg ggtagtgaat ccgccaggat
     5761 tgacttgcgc tgccctacct ctcactagtg aggggcggca gcgcatcaag cggtgagcgc
     5821 actccggcac cgccaacttt cagcacatgc gtgtaaatca tcgtcgtaga gacgtcggaa
     5881 tggccgagca gatcctgcac ggttcgaatg tcgtaaccgc tgcggagcaa ggccgtcgcg
     5941 aacgagtggc ggagggtgtg cggtgtggcg ggcttcgtga tgcctgcttg ttctacggca
     6001 cgtttgaagg cgcgctgaaa ggtctggtca tacatgtgat ggcgacgcac gacaccgctc
     6061 cgtggatcgg tcgaatgcgt gtgctgcgca aaaacccaga accacggcca ggaatgcccg
     6121 gcgcgcggat acttccgctc aagggcgtcg ggaagcgcaa cgccgctgcg gccctcggcc
     6181 tggtccttca gccaccatgc ccgtgcacgc gacagctgct cgcgcaggct gggtgccaag
     6241 ctctcgggta acatcaaggc ccgatccttg gagcccttgc cctcccgcac gatgatcgtg
     6301 ccgtgatcga aatccagatc cttgacccgc agttgcaaac cctcactgat ccgcatgccc
     6361 gttccataca gaagctgggc gaacaaacga tgctcgcctt ccagaaaacc gaggatgcga
     6421 accacttcat ccggggtcag caccaccggc aagcgccgcg acggccgagg tcttccgatc
     6481 tcctgaagcc agggcagatc cgtgcacagc accttgccgt agaagaacag caaggccgcc
     6541 aatgcctgac gatgcgtgga gaccgaaacc ttgcgctcgt tcgccagcca ggacagaaat
     6601 gcctcgactt cgctgctgcc caaggttgcc gggtgacgca caccgtggaa acggatgaag
     6661 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     6721 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     6781 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     6841 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     6901 cagcagggca gtcgccctaa aacaaagttg ggcgaacccg gagcctcatt aattgttagc
     6961 cgttaaaatt aagcccttta ccaaaccaat acttattatg aaaaacacaa tacatatcaa
     7021 cttcgctatt tttttaataa ttgcaaatat tatctacagc agcgccagtg catcaacaga
     7081 tatctctact gttgcatctc cattatttga aggaactgaa ggttgttttt tactttacga
     7141 tgcatccaca aacgctgaaa ttgctcaatt caataaagca aagtgtgcaa cgcaaatggc
     7201 accagattca actttcaaga tcgcattatc acttatggca tttgatgcgg aaataataga
     7261 tcagaaaacc atattcaaat gggataaaac ccccaaagga atggagatct ggaacagcaa
     7321 tcatacacca aagacgtgga tgcaattttc tgttgtttgg gtttcgcaag aaataaccca
     7381 aaaaattgga ttaaataaaa tcaagaatta tctcaaagat tttgattatg gaaatcaaga
     7441 cttctctgga gataaagaaa gaaacaacgg attaacagaa gcatggctcg aaagtagctt
     7501 aaaaatttca ccagaagaac aaattcaatt cctgcgtaaa attattaatc acaatctccc
     7561 agttaaaaac tcagccatag aaaacaccat agagaacatg tatctacaag atctggataa
     7621 tagtacaaaa ctgtatggga aaactggtgc aggattcaca gcaaatagaa ccttacaaaa
     7681 cggatggttt gaagggttta ttataagcaa atcaggacat aaatatgttt ttgtgtccgc
     7741 acttacagga aacttggggt cgaatttaac atcaagcata aaagccaaga aaaatgcgat
     7801 caccattcta aacacactaa atttataaaa aatctaatgg caaaatcgcc caacccttca
     7861 atcaagtcgg gacggccaaa agcaagcttt tggctcccct cgctggcgct cggcgcccct
     7921 tatttcaaac gttaaacatc atgagggaag tggtgatcgc cgaagtatcg actcaactat
     7981 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     8041 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     8101 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     8161 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     8221 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     8281 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     8341 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     8401 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     8461 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     8521 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     8581 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc taggcaggct tatcttggac
     8641 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgttcac tacgtgaaag
     8701 gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc cgacgccgct
     8761 tcgcggcgcg gcttaactca agcgttaga
//
LOCUS       DAZSIO010000589         7053 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.guided.183, whole genome shotgun sequence.
ACCESSION   DAZSIO010000589 DAZSIO010000000
VERSION     DAZSIO010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.guided.183"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            59..1102
                     /locus_tag="ACHX5T_004644"
     CDS             59..1102
                     /locus_tag="ACHX5T_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR8469474.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            1164..1778
                     /gene="prfH"
                     /locus_tag="ACHX5T_004645"
                     /pseudo
     CDS             1164..1778
                     /gene="prfH"
                     /locus_tag="ACHX5T_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="ACHX5T_004646"
     CDS             complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="ACHX5T_004646"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR8469475.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3554..4012
                     /gene="gpt"
                     /locus_tag="ACHX5T_004647"
     CDS             3554..4012
                     /gene="gpt"
                     /locus_tag="ACHX5T_004647"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR8469476.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4104..5348
                     /gene="frsA"
                     /locus_tag="ACHX5T_004648"
     CDS             4104..5348
                     /gene="frsA"
                     /locus_tag="ACHX5T_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR8469477.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5406..5807
                     /gene="crl"
                     /locus_tag="ACHX5T_004649"
     CDS             5406..5807
                     /gene="crl"
                     /locus_tag="ACHX5T_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR8469478.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(5846..6901)
                     /gene="phoE"
                     /locus_tag="ACHX5T_004650"
     CDS             complement(5846..6901)
                     /gene="phoE"
                     /locus_tag="ACHX5T_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR8469479.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         1716 a         1735 c         1817 g         1785 t
ORIGIN      
        1 aattcaagtc gcagcacatg caacttgaag tatgacgagt ctatttacgg aatggtatat
       61 gggcaattat attcgtccct tatccgatgc ggtatttacc atcgcatctg atgacctgtg
      121 gatcgagagt ttagcgatcc aacaattaca caccacggca aatttaccca acatgcagcg
      181 cgtagtgggg atgccagatt tacaccccgg acgcggctac ccgattggcg cagcgttctt
      241 ctccgcaggc cgtttttacc cagcactggt cggcaatgat atcggctgtg gcatggcgct
      301 atggcaaaca gatattctcg ctcgcaaata caacgccgat aagtttgaaa agcgattatc
      361 agctttggat gacgttgctg aagaaagctg gctggaggaa aacctgccgt cagcatttgc
      421 acagcatccg tggcgcagct cgcttggctc tatcggtggt ggcaatcatt tcgctgaact
      481 gcaacaagtt gatcaaatca tcaacgctga actgtttgca ctggcaggtc tggatgcgca
      541 gcatctgcaa ctgctggttc atagcggctc gagggggcta gggcagtcta ttttacagcg
      601 gcatattgcc tcgttttcgc atcatggttt gcctgaaggc agtgacgacg cgctggcgtt
      661 tgcgcgtttt aatcgccacc tgatcgcttt gcgcataatg caacaggtta aggccaccgg
      721 aagtccggtt ctggatgtgg cgcataactt tgttagcgcg tgtcgaatcg gtgatcaaca
      781 aggggtctta catcgtaaag gtgccacgcc tgatgactgt ggtctggtgg ttattcccgg
      841 ttcacgcggt gattactcca ggctggttca gcccgtcagg agtgaggaaa cattgcattc
      901 gctggcgcat ggggctgggc gtaaatgggg gcgcaccgag tgtaaagggc gtctggcagc
      961 gaaatacaca gcgacgcaac tctcgcgtac tgaacttggc agtcgggtaa tttgtcgcga
     1021 taaacaactc atcttcgaag aagcgccaca agcctataaa tcggctgaaa gtgtggtgca
     1081 atgtcagggc aggccggatt aattattcct gtcgcgcggc tgcgtccggt gctaacgctc
     1141 aaaaacagtg gagggaaaaa aggatgatct tgctataact ctcctcagcg caggggccgg
     1201 aagaatgttg tctcgcagtg aaaaaggcgc tggacaggct aattaaagaa gctgcccgac
     1261 aagacgtcgc ggtaacggtg ctggaaacag aaacaggccg ctactctgac acgctgcgtt
     1321 cggcgctgat ttctctggat ggcgacaacg catgggcgtt aagcgaaagc tggtgtggca
     1381 ctattcagtg gatttgcccg agcccgtatc gcccacatca cgggcgtaaa aactggtttc
     1441 tgggcattgg gcgttttacc gctgatgagc aggagcaatc cgatgcgatc cggtatgaga
     1501 cgctgcgttc gtcggggccg ggcggtcaac atgtcaataa aaccgactcg gcggtacgcg
     1561 ccacgcattt ggcatccggt attagcgtga aggttcagtc agagcgcagc cagcatgcta
     1621 acaaacggct ggcgcgattg ctgattgcct ggaagctgga tcagcagcaa taggaaaata
     1681 gcgcggtgct gaaatcacag cggcgaatgt tccatcacca gattgaacgt ggcaacccgc
     1741 gacgaacgtt tacagggatg gcttttatcg aaggataatg aaggatgaaa ctgccggaag
     1801 gcgattaaac gccatccggc agcaaatcaa ataattactt cgccggaatt tctttcagca
     1861 gttcagtcag cagtgtccag taatgaccta cgctttcgat gtgaacttgc tcatccggag
     1921 agtgtggacc ggtgatagtt ggcccgatag aaaccatgtc catttccgga tacggttttt
     1981 tgaacagacc acattccagg cccgcgtgga taatctggat gttcggcgtc ttgttgaaca
     2041 ggcgctgata ggtttcacgt accagatgca tcaccggaga attagcgtcc ggctgccagc
     2101 caggatatgc gcctttcgct tcggttttcg cgccagccag tttacccagc gaatccagca
     2161 tgctcaccac gtagtcttta ccgctgtcga ttagtgaacg gatcaggcag tgaatttcta
     2221 cgttattgtc agtcatggtc accacaccga cgttcaggga ggtttcgacc acaccttttg
     2281 ccacgtcgga attgcggatc acaccgttcg gggtggcgtt cagcagacga ataaaggtat
     2341 cgcgagattt cgcaatcagg gcagctttat cgttcgctac agagtccagc aacaaggcca
     2401 gatttttctc tttctctgcc agctcgtttt tcaggatctc ctgataggta ttcaccagag
     2461 atttcagtgc gtcgacttta tcagctgcga cagcaatggt cgcaaaggct tcacgcggga
     2521 tggcgttacg cagtgtgccg ccgttgaaat cgataaggcg caggtccagt tcttctgcat
     2581 gacccgccag gaagcgcacc agcagtttgt tggcattacc caggccaacg tggatttcac
     2641 cgccggagtg accgcctttc agacctttta aggttaactt gaaggtttca aaaccagctg
     2701 gaaccgcttc acgatctaaa tgcaggttgg aggtgaagtc gatacccccc gcacaaccca
     2761 tgtagatttc accttcttct tcggagtcgg tgttaatcag aatatcagcc tgcaaccagt
     2821 tgctctgtaa gccgaacgca ccgtccatac cggcttcttc ggtcatggtc agcagcactt
     2881 ccagcgggcc gtgaaccacg ttttcgtcag ccagaaccgc cagtgcagag gccataccaa
     2941 tgccgttatc cgcacccagc gtggtgccgc gcgctttaac ccattcgcca tcaatataag
     3001 gctggatagg atctttcgtg aagtcatgca cggtgtcgtt atttttctgc ggcaccatat
     3061 cgaggtgggc ctgtaagacg accggtttac gattttccat acctgcggta gcaggttgac
     3121 gaatcaggat attacctacc tgatcgcgtt cgacatggaa acctttctct tttgcccaac
     3181 caacaatgta ttcagcgagt tgctcttcat gataggacgg gtgaggaata gaacagattt
     3241 tggcaaaaat atcccacagc ggctgtggag ataattgaga cagttcagac acgttaagtc
     3301 tccttgtcga tcacccgcaa aacagtattg caggtcacag ggttagcaga aaatgttgtc
     3361 aacacaagac aggcttgcga gatatgtatt gagaatacca ctttatcccg cgtcagggag
     3421 aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct
     3481 ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc
     3541 tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc
     3601 acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag
     3661 ccgtggcggt ctggtaccgg gggcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     3721 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
     3781 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
     3841 tgctgttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     3901 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggatcga
     3961 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     4021 acgcctggca ttgccgggcg ttgttctttt taacttcagg caggttacaa tagtttccag
     4081 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     4141 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     4201 cgcctgtgca gtcggctctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     4261 gtctgatgtg gatctggcgc ggtattgacc cacgtgaaat cctcgacgtc caggcacgta
     4321 ttgtgatgag cgatgctgaa cgtaccgacg atgatttata cgataccgtg attggctacc
     4381 gtggtggcaa ctggatttat gagtgggcca cccaggcgat ggtgtggcaa caaaaagcct
     4441 gtacggaaga agatccgcaa ctcagtggtc gacactggct gcatgcggct acgttgtaca
     4501 acattgccgc ctatcctcat ctgaaaggag atgacctggc cgagcaagcg caggctttat
     4561 caaaccgcgc ctatgaagag gccgcccagc gtctgccggg cacgatgcgg cagatggagt
     4621 ttaccgtacc cggcggtgcg cccatcaccg gctttttgca tatgccgaaa ggcgatggcc
     4681 cgttcccgac agtattaatg tgtggtggtc tggatgcgat gcagacggac tattacagcc
     4741 tgtatgaacg ttattttgcg ccgcgcggca ttgcgatgct gactattgat atgccgtcgg
     4801 tgggcttttc ttcaaaatgg aagctcaccc aggactccag cctgttgcat cagcacgtct
     4861 taaaggcgct gcctaacgta ccgtgggtgg atcacactcg cgtcgcggcc tttggtttcc
     4921 gtttcggcgc taacgttgcc gtgcgtctgg cataccttga atcgccgcgt ctgaaagcgg
     4981 ttgcctgtct tggtccggta gttcataccc tgttgagtga ttttaagtgc cagcaacagg
     5041 tgccggaaat gtatcttgac gttctggcga gtcgtttggg gatgcatgat gcttccgacg
     5101 aagcgttacg cgtggagctg aatcgctatt cattaaaagt gcaaggattg ctggggcgtc
     5161 gctgcccaac gccaatgtta tcaggctact ggaagaacga tccgttcagc ccggaagagg
     5221 actcacgctt aatcacctca tcatctgctg acggtaaatt attagagatc ccatttaacc
     5281 cggtgtatcg gaattttgac aaaggtcttc aggaaatcac cggctggatc gaaaaacgct
     5341 tgtgttaaaa atttgctaaa ttttgccaat ttggtaaaac agttgcatca caacaggaga
     5401 tagcaatgac gttaccgagt ggacacccga agagcagatt gatcaaaaaa tttaccgcac
     5461 taggcccgta tattcgtgaa ggtaagtgcg aagataatcg attctttttc gattgtctgg
     5521 ctgtatgcgt caacgtgaaa ccggcaccgg aagtgcgtga attctggggc tggtggatgg
     5581 agctggaagc gcaggaatcc cgttttacct acagctacca gtttggtctg ttcgataaag
     5641 caggcgactg gaagagtgtt ccggtaaaag atactgaagt ggttgaacga ctggagcaca
     5701 ccctgcgtga gtttcacgag aagctgcgtg aactgctgac gacgctgaat ctgaagctgg
     5761 aaccggcgaa tgattttcgt gatgaaccgg tgaagttaac ggcgtgagtg aaatgtgccg
     5821 gatgcatcac atccggcaat attcattaaa actgatacgt catcccaacc gcgacagtat
     5881 catcattatt aatattcagc ttattatcgc tatccagttg gttgatttta taatcaacaa
     5941 acgctgacat atttttgttg aaataatacg tagcaccgac gtcgatataa ttgaccagat
     6001 cttcatcacc gatgccctca atatctttcc ctttcgataa gacataaccc agcgatggac
     6061 gcagaccaaa gtcaaactgg tattgagcga ccgcttcaaa gttctgtgtc ttattggcaa
     6121 agccgccagt tattggcgtc attttgcgtg tttcagaata gaaagttgcc agataaatat
     6181 tattggcatc gtatttcaga cctgttgccc atgcttctgc acgcttgcct gtgccacggc
     6241 tttgcaggtt ctgctcgttg gtgcgatctg agttggtata ggccccacta atggcgaaat
     6301 cgctgccgcc aaagtcatat gtcaatgacg tgccgaagcc atcgccgttt tgctttttaa
     6361 cgtcgcggtt ttcgtttttc ccttgatatt gcagggttaa gttcaggcca tcgataacgc
     6421 cgaagaagtc ggtgttccga tacgtcgcca gaccgctggc gcgtttggtc ataaagttgt
     6481 cggtctgcgc cgaggaatcg ccaccaaatt ccgggaacat atcggtccag gcttccacgt
     6541 catacaacgc gcccaggtta cgaccataat cgaaagaacc caaatcttta tatttcaacc
     6601 cggcaaaagc gagacgcgtt ttttgctgtg cagtatcact ctccgcttta tttccggcaa
     6661 actccgcttc ccaccgacca taaccagtca gttgatcgtt aatttgagtt tcacctttaa
     6721 aaccaaaacg gatataactc tggtcgccat ctttactggc gttatcactc atataatgca
     6781 tggctttaac tttgccatag acatccagtt tattaccgtc tttattatat atttctgcag
     6841 cctgtacaga tgcagatgcc acaatgccca tcaccactaa tgccagagtg ctctttttca
     6901 ttttcattcc tgattttaat taacgcgcga atattcagcg ggagagcccc gttgaaaaca
     6961 ggaaagtttt taacctgaga ttgttaaaga tatattacag attaatgata ttctgaaaat
     7021 gggtaatgct gccaacttac tgatttagtg tat
//
LOCUS       DAZSIO010000590         4950 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.guided.224, whole genome shotgun sequence.
ACCESSION   DAZSIO010000590 DAZSIO010000000
VERSION     DAZSIO010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.guided.224"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            354..2162
                     /gene="frdA"
                     /locus_tag="ACHX5T_004651"
     CDS             354..2162
                     /gene="frdA"
                     /locus_tag="ACHX5T_004651"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR8469480.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            2155..2889
                     /gene="frdB"
                     /locus_tag="ACHX5T_004652"
     CDS             2155..2889
                     /gene="frdB"
                     /locus_tag="ACHX5T_004652"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR8469481.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            2900..3295
                     /gene="frdC"
                     /locus_tag="ACHX5T_004653"
     CDS             2900..3295
                     /gene="frdC"
                     /locus_tag="ACHX5T_004653"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR8469482.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            3306..3665
                     /gene="frdD"
                     /locus_tag="ACHX5T_004654"
     CDS             3306..3665
                     /gene="frdD"
                     /locus_tag="ACHX5T_004654"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR8469483.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            3728..4860
                     /locus_tag="ACHX5T_004655"
                     /pseudo
     CDS             3728..4860
                     /locus_tag="ACHX5T_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
BASE COUNT         1225 a         1281 c         1319 g         1125 t
ORIGIN      
        1 ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga caagggcacg
       61 aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac agcggtgggc
      121 agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc ttcagtgata
      181 atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact tatccatcag
      241 actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg taactttcag
      301 gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat gtcgtgcaaa
      361 cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt gctgcaattg
      421 ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta tacccgatgc
      481 gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat catgacagct
      541 tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag caggatgtcg
      601 tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg tggggatgcc
      661 catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc atgaaaatcg
      721 agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg ctgttccaga
      781 cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg gatattctgg
      841 ttgatgatgg tcatgttcgc ggcctggtag caataaacat gatggaaggc acgttggtgc
      901 agatccgtgc taacgcggtc gttatggcta ccggcggtgc gggtcgcgtt tatcgttaca
      961 acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc cacggcgttc
     1021 cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt tccggtatcc
     1081 tgatgaccga aggctgccgt ggtgaaggcg gtattctggt caacaaaaat ggctaccgtt
     1141 atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa aacaaatata
     1201 tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg cgtaaaggca
     1261 acaccatctc cacgccgcgt ggtgatgtgg tttatctcga cctgcgtcac ctcggcgaga
     1321 aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac gttggcgtcg
     1381 atccggttaa agaaccgatt ccggtacgtc cgactgcaca ctacaccatg ggcggtatcg
     1441 aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt gaatgttcct
     1501 ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa ctggtggtct
     1561 tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt aatggcaacg
     1621 aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat ctagttaacc
     1681 aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca atggaagaag
     1741 gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag ctggcagagc
     1801 tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc aacaccgacc
     1861 tgctctacac cattgaactg agccacggtc tgaacgttgc tgaatgtatg gcgcactccg
     1921 caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt tgcaccgagc
     1981 gtgacgacgt caactttctc aaacacaccc tcgccttccg cgatgctgat ggcacgactc
     2041 gcctggagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc gtttacggtg
     2101 gcgaagcgga tgcagccgat aaggcggaag cagccaataa taaggagaag gcgaatggct
     2161 gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga taccgcaccg
     2221 catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga tgcgctgggc
     2281 tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg tatggcgatt
     2341 tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa aaccttcctg
     2401 cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat tgaacgcgat
     2461 ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc gtacatcatc
     2521 ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca gatggcaaag
     2581 tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg cccgcagttt
     2641 ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg ttataacgaa
     2701 gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca gaacggtgta
     2761 tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt cgatccggct
     2821 gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc gaccctgaaa
     2881 ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc caatgacgtc
     2941 cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag gcacggcggt
     3001 tccggctgtg tggttcagca ttgaactgat tttcgcgctg tttgccctga aaaatggccc
     3061 ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga tcattaacct
     3121 gatcactctg acggcagctc tgctgcacac caaaacctgg tttgaactgg caccgaaagc
     3181 ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca aaagtctctg
     3241 ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact ggtaaggagc
     3301 ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg ggcctcttcg
     3361 gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg gtgggtattc
     3421 tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt ctggcgttcg
     3481 cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg ctgtggtgtg
     3541 gtttacaccg tatgcaccac gcgatgcacg atctgaaaat ccacgtgcct gcgggcaaat
     3601 gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt gtcgttacaa
     3661 tctaacgcat cgccaatgta aatccggccc gcctatggcg ggccgttttg tatggaaacc
     3721 agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt actccacatt
     3781 tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc ttatagagca
     3841 acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt attactttac
     3901 ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt tgtttgagtt
     3961 aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg ctcgagggga
     4021 aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttaccgcta aacagtggaa
     4081 tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat tgcaggtgcc
     4141 ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc agcctgcatg
     4201 ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg gcgcactggc
     4261 tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct tccagccact
     4321 caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt acgcctgggg
     4381 atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg aagcttatgg
     4441 tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttac cccccttgat
     4501 atcaatgaga aaacgcttca acaagggata caactggcac aatctcgcta ctggcaaacc
     4561 ggcgatatgt atcagggcct gggctgggaa atgctggact ggccggtcaa tcctgacagc
     4621 atcattaacg gcagtgacaa taaaattgca ctggcagcac gcccggtaaa agcgattaag
     4681 cccccaactc ctgcagtacg cgcatcatgg gtacataaaa cgggggcgac cggcggattt
     4741 ggtagctatg tcgcgtttat tccagaaaaa gagctgggta tcgtgatgct ggctaacaaa
     4801 aactatccca atccagcgag agtcgccgcc gcctgtcaga ttctcaacgc tctacagtaa
     4861 aatttcatcg ggtccgaatt ttcggacctt ttctgcgctt ttccttgctg tcatctacac
     4921 ttagaaaaaa accagtaagg aaacatttat
//
LOCUS       DAZSIO010000591         3063 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.guided.285, whole genome shotgun sequence.
ACCESSION   DAZSIO010000591 DAZSIO010000000
VERSION     DAZSIO010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.guided.285"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            41..430
                     /locus_tag="ACHX5T_004656"
                     /pseudo
     CDS             41..430
                     /locus_tag="ACHX5T_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(408..1031)
                     /gene="tetR(B)"
                     /locus_tag="ACHX5T_004657"
     CDS             complement(408..1031)
                     /gene="tetR(B)"
                     /locus_tag="ACHX5T_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFR8469484.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1113..2318
                     /gene="tet(B)"
                     /locus_tag="ACHX5T_004658"
     CDS             1113..2318
                     /gene="tet(B)"
                     /locus_tag="ACHX5T_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFR8469485.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2431..3024)
                     /gene="tetC"
                     /locus_tag="ACHX5T_004659"
     CDS             complement(2431..3024)
                     /gene="tetC"
                     /locus_tag="ACHX5T_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HFR8469486.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          814 a          578 c          626 g         1045 t
ORIGIN      
        1 tctgaacata aaacactatc aataagttgg agtcattacc aaccatggcg tacatgccaa
       61 agtttccacg ccagtgcatt tacgaaaagc acgtacttgc tatgatcatt tagctggcga
      121 agttgccgtt aagatctatg attccctttg tcaacagcaa tggatcactg aaaatggttc
      181 aatgatcaca ttaagtggta ttcaatattt tcatgaaatg ggaattgacg ttccttccaa
      241 acattcacgt aaaatctgtt gtgcgtgttt agattggagt gaacgccgtt tccatttagg
      301 tgggtacgtt ggagccgcat tattttcgct ttatgaatct aaagggtggt taactcgaca
      361 tcttggttac cgtgaagtta ccatcacgga aaaaggttat gctgctttta agacccactt
      421 tcacatttaa gttgtttttc taatccgcat atgatcaatt caaggccgaa taagaaggct
      481 ggctctgcac cttggtgatc aaataattcg atagcttgtc gtaataatgg cggcatacta
      541 tcagtagtag gtgtttccct ttcttcttta gcgacttgat gctcttgatc ttccaatacg
      601 caacctaaag taaaatgccc cacagcgctg agtgcatata atgcattctc tagtgaaaaa
      661 ccttgttggc ataaaaaggc taattgattt tcgagagttt catactgttt ttctgtaggc
      721 cgtgtaccta aatgtacttt tgctccatcg cgatgactta gtaaagcaca tctaaaactt
      781 ttagcgttat tacgtaaaaa atcttgccag ctttcccctt ctaaagggca aaagtgagta
      841 tggtgcctat ctaacatctc aatggctaag gcgtcgagca aagcccgctt attttttaca
      901 tgccaataca atgtaggctg ctctacacct agcttctggg cgagtttacg ggttgttaaa
      961 ccttcgattc cgacctcatt aagcagctct aatgcgctgt taatcacttt acttttatct
     1021 aatctagaca tcattaattc ctaatttttg ttgacactct atcattgata gagttatttt
     1081 accactccct atcagtgata gagaaaagtg aaatgaatag ttcgacaaag atcgcattgg
     1141 taattacgtt actcgatgcc atggggattg gccttatcat gccagtcttg ccaacgttat
     1201 tacgtgaatt tattgcttcg gaagatatcg ctaaccactt tggcgtattg cttgcacttt
     1261 atgcgttaat gcaggttatc tttgctcctt ggcttggaaa aatgtctgac cgatttggtc
     1321 ggcgcccagt gctgttgttg tcattaatag gcgcatcgct ggattactta ttgctggctt
     1381 tttcaagtgc gctttggatg ctgtatttag gccgtttgct ttcagggatc acaggagcta
     1441 ctggggctgt cgcggcatcg gtcattgccg ataccacctc agcttctcaa cgcgtgaagt
     1501 ggttcggttg gttaggggca agttttgggc ttggtttaat agcggggcct attattggtg
     1561 gttttgcagg agagatttca ccgcatagtc ccttttttat cgctgcgttg ctaaatattg
     1621 tcgctttcct tgtggttatg ttttggttcc gtgaaaccaa aaatacacgt gataatacag
     1681 ataccgaagt aggggttgag acgcaatcga attcggtata catcacttta tttaaaacga
     1741 tgcccatttt gttgattatt tatttttcag cgcaattgat aggccaaatt cccgcaacgg
     1801 tgtgggtgct atttaccgaa aatcgttttg gatggaatag catgatggtt ggcttttcat
     1861 tagcgggtct tggtctttta cactcagtat tccaagcctt tgtggcagga agaatagcca
     1921 ctaaatgggg cgaaaaaacg gcagtactgc tcggatttat tgcagatagt agtgcatttg
     1981 cctttttagc gtttatatct gaaggttggt tagttttccc tgttttaatt ttattggctg
     2041 gtggtgggat cgctttacct gcattacagg gagtgatgtc tatccaaaca aagagtcatc
     2101 agcaaggtgc tttacaggga ttattggtga gccttaccaa tgcaaccggt gttattggcc
     2161 cattactgtt tgctgttatt tataatcatt cactaccaat ttgggatggc tggatttgga
     2221 ttattggttt agcgttttac tgtattatta tcctgctatc gatgaccttc atgttaaccc
     2281 ctcaagctca ggggagtaaa caggagacaa gtgcttagtt atttcgtcac caaatgatgt
     2341 tattccgcga aatataatga ccctcttgat aacccaagag ggcatttttt acgataaaga
     2401 agatttagct tcaaataaaa cctatctatt ttatttatct ttcaagctca ataaaaagcc
     2461 gcggtaaata gcaataaatt ggcctttttt atcggcaagc tcttttaggt ttttcgcatg
     2521 tattgcgata tgcataaacc agccattgag taagttttta agcacatcat catcataagc
     2581 tttaagttgg ttctcttgga tcaatttgct gacaatggcg tttaccttac cagtaatgta
     2641 ttcaaggcta attttttcaa gttcattcca accaatgata ggcatcactt cttggatagg
     2701 gataaggttt ttattattat caataatata atcaagataa tgttcaaata tactttctaa
     2761 ggcagaccaa ccatttgtta aatcagtttt tgttgtgatg taggcatcaa tcataattaa
     2821 ttgctgctta taacaggcac tgagtaattg ttttttattt ttaaagtgat gataaaaggc
     2881 acctttggtc accaacgctt ttcccgagat ctcatctatt gaaacagctt gatagccttt
     2941 ttcaacaaac aatattcgtg ctgagttaac cagtgattga taggtactct taaaattttc
     3001 ttgttgatga tttttatttt ccatgataga tttaaaataa cataccgtca gtatgtttat
     3061 ggt
//
LOCUS       DAZSIO010000592         2124 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.guided.331, whole genome shotgun sequence.
ACCESSION   DAZSIO010000592 DAZSIO010000000
VERSION     DAZSIO010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.guided.331"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            123..596
                     /gene="dfrA1"
                     /locus_tag="ACHX5T_004660"
     CDS             123..596
                     /gene="dfrA1"
                     /locus_tag="ACHX5T_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR8469487.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            691..1215
                     /gene="sat2"
                     /locus_tag="ACHX5T_004661"
     CDS             691..1215
                     /gene="sat2"
                     /locus_tag="ACHX5T_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR8469488.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            1273..2111
                     /gene="aadA1"
                     /locus_tag="ACHX5T_004662"
                     /pseudo
     CDS             1273..2111
                     /gene="aadA1"
                     /locus_tag="ACHX5T_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT          590 a          489 c          526 g          519 t
ORIGIN      
        1 gtgtcttgta tttttaaaga aagtctattt aatacaagtg attatattaa ttaacggtaa
       61 gcatcagcgg gtgacaaaac gagcatgctt actaataaaa tgttaacctc tgaggaagaa
      121 ttgtgaaact atcactaatg gtagctatat cgaagaatgg agttatcggg aatggccctg
      181 atattccatg gagtgccaaa ggtgaacagc tcctgtttaa agctattacc tataaccaat
      241 ggctgttggt tggacgcaag acttttgaat caatgggagc attacccaac cgaaagtatg
      301 cggtcgtaac acgttcaagt tttacatctg acaatgagaa cgtattgatc tttccatcaa
      361 ttaaagatgc tttaaccaac ctaaagaaaa taacggatca tgtcattgtt tcaggtggtg
      421 gggagatata caaaagcctg atcgatcaag tagatacact acatatatct acaatagaca
      481 tcgagccgga aggtgatgtt tactttcctg aaatccccag caattttagg ccagttttta
      541 cccaagactt cgcctctaac ataaattata gttaccaaat ctggcaaaag ggttaacaag
      601 tggcagcaac ggattcgcaa acctgtcacg ccttttgtac caaaagccgc gccaggtttg
      661 cgatccgctg tgccaggcgt taggcgtcat atgaagattt cggtgatccc tgagcaggtg
      721 gcggaaacat tggatgctga gaaccatttc attgttcgtg aagtgttcga tgtgcaccta
      781 tccgaccaag gctttgaact atctaccaga agtgtgagcc cctaccggaa ggattacatc
      841 tcggatgatg actctgatga agactctgct tgctatggcg cattcatcga ccaagagctt
      901 gtcgggaaga ttgaactcaa ctcaacatgg aacgatctag cctctatcga acacattgtt
      961 gtgtcgcaca cgcaccgagg caaaggagtc gcgcacagtc tcatcgaatt tgcgaaaaag
     1021 tgggcactaa gcagacagct ccttggcata cgattagaga cacaaacgaa caatgtacct
     1081 gcctgcaatt tgtacgcaaa atgtggcttt actctcggcg gcattgacct gttcacgtat
     1141 aaaactagac ctcaagtctc gaacgaaaca gcgatgtact ggtactggtt ctcgggagca
     1201 caggatgacg cctaacaatt cattcaagcc gacaccgctt cgcggcgcgg cttaattcag
     1261 gagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     1321 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     1381 gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta
     1441 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     1501 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     1561 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     1621 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     1681 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     1741 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     1801 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     1861 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     1921 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     1981 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg ttcactacgt gaaaggcgag
     2041 atcaccaagg tagtcggcaa ataatgtcta acaattcgtt caagccgacg ccgcttcgcg
     2101 gcgcggctta actcaagcgt taga
//
LOCUS       DAZSIO010000593         2121 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2307424
            SAMN43277783-rid23494893.guided.333, whole genome shotgun sequence.
ACCESSION   DAZSIO010000593 DAZSIO010000000
VERSION     DAZSIO010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277783
            Sequence Read Archive: SRR30464066
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396417.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396417.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:47:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 229 of 479
            Pseudo Genes (incomplete)         :: 152 of 479
            Pseudo Genes (internal stop)      :: 167 of 479
            Pseudo Genes (multiple problems)  :: 64 of 479
            Pseudo Genes (short protein)      :: 1 of 479
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277783-rid23494893.guided.333"
                     /strain="M2307424"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-09-22"
                     /collected_by="FSS"
     gene            123..596
                     /gene="dfrA1"
                     /locus_tag="ACHX5T_004663"
     CDS             123..596
                     /gene="dfrA1"
                     /locus_tag="ACHX5T_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR8469489.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            691..1215
                     /gene="sat2"
                     /locus_tag="ACHX5T_004664"
     CDS             691..1215
                     /gene="sat2"
                     /locus_tag="ACHX5T_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR8469490.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            1273..2108
                     /gene="aadA1"
                     /locus_tag="ACHX5T_004665"
                     /pseudo
     CDS             1273..2108
                     /gene="aadA1"
                     /locus_tag="ACHX5T_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT          587 a          489 c          526 g          519 t
ORIGIN      
        1 gtgtcttgta tttttaaaga aagtctattt aatacaagtg attatattaa ttaacggtaa
       61 gcatcagcgg gtgacaaaac gagcatgctt actaataaaa tgttaacctc tgaggaagaa
      121 ttgtgaaact atcactaatg gtagctatat cgaagaatgg agttatcggg aatggccctg
      181 atattccatg gagtgccaaa ggtgaacagc tcctgtttaa agctattacc tataaccaat
      241 ggctgttggt tggacgcaag acttttgaat caatgggagc attacccaac cgaaagtatg
      301 cggtcgtaac acgttcaagt tttacatctg acaatgagaa cgtattgatc tttccatcaa
      361 ttaaagatgc tttaaccaac ctaaagaaaa taacggatca tgtcattgtt tcaggtggtg
      421 gggagatata caaaagcctg atcgatcaag tagatacact acatatatct acaatagaca
      481 tcgagccgga aggtgatgtt tactttcctg aaatccccag caattttagg ccagttttta
      541 cccaagactt cgcctctaac ataaattata gttaccaaat ctggcaaaag ggttaacaag
      601 tggcagcaac ggattcgcaa acctgtcacg ccttttgtac caaaagccgc gccaggtttg
      661 cgatccgctg tgccaggcgt taggcgtcat atgaagattt cggtgatccc tgagcaggtg
      721 gcggaaacat tggatgctga gaaccatttc attgttcgtg aagtgttcga tgtgcaccta
      781 tccgaccaag gctttgaact atctaccaga agtgtgagcc cctaccggaa ggattacatc
      841 tcggatgatg actctgatga agactctgct tgctatggcg cattcatcga ccaagagctt
      901 gtcgggaaga ttgaactcaa ctcaacatgg aacgatctag cctctatcga acacattgtt
      961 gtgtcgcaca cgcaccgagg caaaggagtc gcgcacagtc tcatcgaatt tgcgaaaaag
     1021 tgggcactaa gcagacagct ccttggcata cgattagaga cacaaacgaa caatgtacct
     1081 gcctgcaatt tgtacgcaaa atgtggcttt actctcggcg gcattgacct gttcacgtat
     1141 aaaactagac ctcaagtctc gaacgaaaca gcgatgtact ggtactggtt ctcgggagca
     1201 caggatgacg cctaacaatt cattcaagcc gacaccgctt cgcggcgcgg cttaattcag
     1261 gagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     1321 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     1381 gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta
     1441 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     1501 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     1561 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     1621 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     1681 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     1741 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     1801 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     1861 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     1921 ccggcccagt atcagcccgt catacttgaa gctaggcagg cttatcttgg acaagaagat
     1981 cgcttggcct cgcgcgcaga tcagttggaa gaatttgttc actacgtgaa aggcgagatc
     2041 accaaggtag tcggcaaata atgtctaaca attcgttcaa gccgacgccg cttcgcggcg
     2101 cggcttaact caagcgttag a
//
